LOCUS       DAOTIQ010000001       118715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_87_length_118715_cov_7.954593, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000001 DAOTIQ010000000
VERSION     DAOTIQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 118715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 118715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118715
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87_length_118715_cov_7.954593"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="P5541_00005"
     CDS             complement(<1..420)
                     /locus_tag="P5541_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70388.1"
                     /translation="MKRNSSSTIIATFIVFTLFSAPPAHSSLLAPVYPGSVNIEHGQG
                     PIGNPYVPDTPMARQFYSKDDIDKVLDFYVSHFGEPHHTTGGKGIYHEWKLKEIPGLI
                     GRMYIATDNREFSTEKLPDQELLSNLVYEPSSGVLVRL"
     gene            1023..3452
                     /locus_tag="P5541_00010"
     CDS             1023..3452
                     /locus_tag="P5541_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRV70389.1"
                     /translation="MNRRSVLFVLCLLMLSIILVSSPSEASGSGYWKLIDVDVEHHET
                     RIENSDGSKLLGTISGGPGQFEATFLDQKRNWKMEYSVSGSWGEIPEILILREEVSVP
                     LELSVGSYSISHPSDVGPFAELVVYFSYSSDSVEEARPRNFAGQIEVSDKGPSHVTAT
                     AKLLPPGPRSGNSQYFVVEVTARNLSSMHYWSKTFKYVYAWQAGEPPAEADRAVEYAV
                     KAASDPRTVSPDGESTVEVSALLYEYDPGDSLSSRPVAGKTIYFTVNEQDGVIPGTLS
                     SASAVTDEYGNATVTFTAPEGSLLKDARLVVTSATVTARAEELDVEDMAYINFTPERG
                     KVFVQPGISGIVSSHGMVPPDRRFPALIAANFEDEDLQPLKDTEVTFSIREGDNHGML
                     RSPDGIEARKVVTRTDPEGWAEVQYFYASDEPPVDPVTETIEIRSRKMILPLTAEVTT
                     GFNLVFERMESAYEGKGTVNAGEEIPFRVNIKDAWNPGVDLEKVVGFWGVDERSGSEM
                     LFVRLEVENIGTVPDFLLDQLKLEKYPEALFSEDMQVRSFRDKGEMNMLWMSGNSLKG
                     YQGYPRIRPMTTGNHYYEARLSLVDGSGREVFRTNHPAARAYFNIETGLPADAMQIFF
                     LENPFRADTAEAKILRTALDVMGFGTVMSVVDAMYAINNGNVKELFSLLFSEAKGAIL
                     SKTGDISAAKKQMVDAYSSIALAEKIDFEIRKDRTGPIAAMEEGIFESLLDAFGLEKG
                     QLVVLRGRGEQKLSVKTEKGPKQIPSKEREYFTDAETGITSLRSGESAFFIIPRDMTV
                     EAGSESEFSRF"
     gene            3513..3926
                     /locus_tag="P5541_00015"
     CDS             3513..3926
                     /locus_tag="P5541_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRV70390.1"
                     /translation="MKIHTGYLWFDTKNRREIVPITRDVEKIMLESGISEGFVLVSAM
                     HITSGIIVNDDEQGLHQDIWAWLEELAPAKPDYRHHRTGEDNADAHLKRILVHHQAMI
                     PVTKGKLDLGPWERVFYLEADGQRRKRVVVKVMGE"
     gene            4065..5639
                     /locus_tag="P5541_00020"
     CDS             4065..5639
                     /locus_tag="P5541_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRV70391.1"
                     /translation="MSPYISIQDLSYTLPDGRVLFKDISESFGGEFSALAGRNGSGKT
                     ILAKIMAGLISPGSGRVIRSGTVCYLDQLVDPSEYSTVADLAGKEKILNSLHRITAGY
                     PEEGDLEIADGNWDMEERLLRELDISGLGYLLPDLPATRLSGGECTRISLIGAFLSGA
                     DFLILDEPTNHLDQPNRKALIEYLGTWKGGLLVISHDRAVLEVVERIFELSSGGLDSY
                     GGNLDVFLEARRLQRSAALDNLERVKTERKRAKAERSMLLERQQRRSSAGRRSAPDSG
                     IPRIAMNAIRSMAKKTEGKTRSAAAEKVEVLAAEEKKAFLEARIEDTVVLIPPRCAVH
                     AGKIVLRLDEVVLPFGSHGEPISLTAAGPERIAVTGPNGSGKSTLLRVLMGETRPLSG
                     SCRIMVDYAYLDQFTSVPLDISPIEHLCAADPGMEQGEAGTRLAQVGISREMLRVKNR
                     NLSGGERLKVALLAAIHRVPVPQLLILDEPTNHLDLDSVESVEKLLCAYTGALVVVSH
                     DRRLLENIAISRELEM"
     gene            5732..6361
                     /locus_tag="P5541_00025"
     CDS             5732..6361
                     /locus_tag="P5541_00025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV70392.1"
                     /translation="MSPDMNESVHGESASQYDEEVKAYSSYGHEVIFGMGFEFVREGE
                     KLLDIGIGTGLASINFSGIGLKVYGLDNSRKMLDACLSKMFTEELVLCDVTREAIPYE
                     DNFFDHVLCCGVLHFWGDMKALFSEVGRVMKSGGIFAFSIAPQETTSDYIHEPTAWGV
                     PIFRHAPGYIAELLETNGMELLKEQRLLIKGADKVHFDMLFSVLISRRI"
     gene            6496..7356
                     /locus_tag="P5541_00030"
     CDS             6496..7356
                     /locus_tag="P5541_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRV70393.1"
                     /translation="MKVSRKSPTASVREAYPVPFRSLAPFLLITFGLAWSILALYIFA
                     QEPMTAMFGQLTGNHPLFFLAVYSPAIAAFALVFRNGGFPGLGRFLGRALLWRCSRGW
                     YVFLLVGVPLIFIAGSAIRGNLFTEPFPFGSFQALILGLFLASIKGPVEEFGWRGLAL
                     PLLQRKFSPIWAGLILGAIWGLWHMPAFLLSGTQQSEWSFAAFFAGCLAISVIATSLF
                     NGSRGSILLSAFFHFTLMNPIFPDAQPYDTYMLIVAACLIVWRDRKEMFTREGSVTEV
                     IPGGVEAANP"
     gene            7484..8272
                     /locus_tag="P5541_00035"
     CDS             7484..8272
                     /locus_tag="P5541_00035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV70394.1"
                     /translation="MDEPSPRFWEIFFEVFENLPRQGPGSRSCAARALGLCRGLPEFP
                     AILDLGCGVGGQTLQIAELTSGSIIAVDSHAPSVERLRTAIAERGLSHRVSAVVGDMA
                     SPEQPPESFDLVWSEGALYNIGLENALSVCHVMLRPGGYLAFTDAVWRREDPLPEVKA
                     VFDMDYPTMGWLADDLAAIKDRGFELAGHFTLPDEAWWDDFYDPMEVRIAELRGKYAD
                     DADAPAILDKLAEEPRMHRLYSDFYAYEFFVARRPAAAPRPVTS"
     gene            complement(8331..10154)
                     /locus_tag="P5541_00040"
     CDS             complement(8331..10154)
                     /locus_tag="P5541_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70395.1"
                     /translation="MPVKGKKILAVFSTAVILAFLSPEILSAVTFESKPADGGKVSIT
                     KTQPEAASSKTEQTSGKPLLYSLELAKSHKERLAESLNLMHLTGLQAVRAINLLKEID
                     GKDLPDNQILARLKYADASLGAAENAANKVKNDIEIIRIASRYDPTLRHEIALFFQNY
                     FAIPAMAEVIFSSSLSDSASNSDIRSSGKSDVAFHPTVLDPIKSIERIEVADATADST
                     SFSENAPIATIRPHGGFFESCMQTIYSAAHTLGSVTSKMKKDIGEGISSLTGAVGNVH
                     TKIGNVVGQKNWANIMAGTKFVATTGGAVVALVVAAPVVTTAAGMTGLMLATGASFTG
                     AGLTLVNDLQTIENENPDPMVKNLDDAVAKINQGTAFIGLASGSDTFVNLLGVTGEEL
                     AGSTPGQEMSNEQLAKFLDQPEHKNALKNQGVLIEMLNDNLILVKPVVSSFATMTDDQ
                     IWALFPTSAQVGMDGVKPKLDKSKSDNLSATYTKGWGVGISGRHCVIHLTLYGSSEIS
                     KEVWNALRGTVKGETVKNVGDDAYIMDLGPDNSYRAVASFRNASVMMTDCIFNQTSDG
                     IKVTRLAVNWNYVKWIFKNMEALPAAPVPAGVETSIEKLRK"
     gene            complement(10243..11274)
                     /locus_tag="P5541_00045"
     CDS             complement(10243..11274)
                     /locus_tag="P5541_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70396.1"
                     /translation="MKIWFKRWLAYLFIPFGIVLLALPISTQRVSYQEREALFLGVTG
                     TIREINGEQTPLPVIKEEDLSGFPSLKTLVDEISQKMDRFGWEHTPILQRPEFPVEEW
                     EGFVSLNAPGGKFLLWSRIFEGRISLTGMQVYEEIPFLEEGRRITGLILLFAGLYSLF
                     SAYPKRGSGIYIGRKRDVIIWDTVITGFAVLFSLWFLDMAFALLFGVAPKWKEEISWG
                     MGTFMVVFANPFLALATSAMSAQVLQINSDGIILKGLFIKSEVKWPDMETIRVTESFS
                     PRKSAGVASSHRLIKFLVIRGRSGRISVMEPPSASTKHKIMAEIREKAPARFGNSIDM
                     AEKEWVALF"
     gene            11475..11897
                     /locus_tag="P5541_00050"
     CDS             11475..11897
                     /locus_tag="P5541_00050"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRV70397.1"
                     /translation="MKRVLFLCTHNSCRSQMAEGWGRRLKSETMEVWSAGTEPGKVNP
                     VAVRVMGESGVDISRHQSKHVEELIHIPFDFVITLCGDARDTCPIFPGEATRIIHRGF
                     DDPSRAEGTEEEVLAFFRRVRDEIRDYVEGMPDNIGEE"
     gene            12008..12427
                     /gene="speD"
                     /locus_tag="P5541_00055"
     CDS             12008..12427
                     /gene="speD"
                     /locus_tag="P5541_00055"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRV70398.1"
                     /translation="MDPRGIHYIVEASGCSEVIGEVDRMQEILVEAARKANTHVWAVS
                     FHRFPPNGVSGVVVISESHLSVHTWPEAGYLALDIYTCGAASLPEEAVKHVLEKVHAG
                     HTHISELTRGLDDDGDRIYYHSLITWEEELQKGKPKK"
     gene            12424..13602
                     /locus_tag="P5541_00060"
     CDS             12424..13602
                     /locus_tag="P5541_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70399.1"
                     /translation="MTAIPPQAVTSFPGAAISLRGVRKNYGGAEVVRGVDLDIPRSEI
                     LVLIGPSGCGKTTILRMINRLVEPSGGSILIDGEDTSKMDPVTLRRRMGYVIQGIGLF
                     PHMRISANVSVVPRLKGVPGGKAMMLAGKALDLVGLPKEEFGDKWPRQLSGGQQQRVG
                     VARALAGDPEIILMDEPFGALDPISRIALQDEFLDLQARLGKTIVFVTHDINEAMKMG
                     SRIAIMRDGAIAQEGEPLHLLSSPADDFVADFVGAGNPLSMFSFMKVRDVPVRSEGLP
                     VVDIAATIDDARRVAHDTSLKFCRDRFVYVVADGRLCGSARVDNPGKSDGHDPVLDIL
                     ETASSVRTTNTVQEALGLMVSSGIANVAVTGERGKFLGVLSFGDMVDIIQQQREKNSR
                     "
     gene            complement(13599..14423)
                     /gene="nudC"
                     /locus_tag="P5541_00065"
     CDS             complement(13599..14423)
                     /gene="nudC"
                     /locus_tag="P5541_00065"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRV70400.1"
                     /translation="MESYTFGNALEGCFVFRGDRILVKTGPRPSLPQIAEIRNMGCST
                     ISGKTAAGQRPGNCWLEVGECGPIPGDMEFVSLRDIWGILGEKAFFEAGRAFQLKEWH
                     RQNQYCGQCGSPMEDSKAETARMCPNCEMISYPPVSPAIIVAVEKGETLLLARSPRFP
                     KGRYSILAGFVEPGETLEETVERELMEEVSIKVKSIKYFGSQPWPFPHSLMVGFTAEW
                     ESGEIKIDGVEIEDADWYRIDSFPEIPPSISISRKLIEDFIKRKGQPAHPVAASRD"
     gene            14527..15699
                     /locus_tag="P5541_00070"
     CDS             14527..15699
                     /locus_tag="P5541_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRV70401.1"
                     /translation="MKDLKVHARNVDEQTMDQIRAITSHPAVDGPVSIMPDCHVGAGC
                     VIGFTGRFRDAVIPNVVGVDIGCGVMLSPMDGVDRGNARFGDLDSHIRKVVPLGPNHH
                     GGRSVFDRFGGRFPRRAGRMEELAGLADDFYGRILRNRTSAPALTQLGTLGSGNHFVE
                     VDEDGDGHLFLSVHTGSRNFGYQVAGYFQDMARSFLRGSGLGEGIPQGLEYLPMDRGG
                     RDYMKWLAVAQEFAEHNRFMILDSVLGFYGLKPDMASITDSVHNYISDRDGIVRKGAI
                     SARNGEKVVIPLSMSSGIVRGTGRGNPAYNCSAPHGAGRLFGRKEMKRRLSDGDISME
                     MFQRSMKGIFSTSVCSATIDESPMAYRNWEDIQEEVAETVDVSAILRPVYNLKAAG"
     gene            complement(15739..15814)
                     /locus_tag="P5541_00075"
     tRNA            complement(15739..15814)
                     /locus_tag="P5541_00075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15778..15780),aa:Glu,seq:ctc)
     gene            complement(15826..15900)
                     /locus_tag="P5541_00080"
     tRNA            complement(15826..15900)
                     /locus_tag="P5541_00080"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15865..15867),aa:Gln,seq:ctg)
     gene            16072..17337
                     /locus_tag="P5541_00085"
     CDS             16072..17337
                     /locus_tag="P5541_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRV70402.1"
                     /translation="MNVDSRSFRELLEKRPLNVQAAFGTEPLALVSGRDIAAAARKTG
                     SIVMAANVRNPLTIKGILKAAKEMDAVVMLELAKSEATYCGCTFDNVPGLAARYSAEL
                     GGGVVFGLHVDHYAIKSRDDLLRSIAHLRQLVENGWTSVAIDASHNPDWENLCFTRDV
                     AMHVPPYLGLEAEVGEIKGPGEITTVEEALFFVGGLNSWGVFPDFLAISNGSKHGTYD
                     SSLGEEEGIDLSRTKEIAESIAPFGAVIAQHGISGTPLDKVSSFVNFGINKGNVGTLW
                     QNIVFGLEMDPSTGNALVRDDAYVKNNSRGVPPELWERMVTWADDQGFSRKSGDYKKA
                     NKPFHNEIMALPEKIRESIVEDTADWARRFIKAFNAEGSAEALLEVVSKRPDHNAAPE
                     REIIRSRSLFTKESAPGDVGNRDGKDYSD"
     gene            17437..19152
                     /locus_tag="P5541_00090"
     CDS             17437..19152
                     /locus_tag="P5541_00090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRV70403.1"
                     /translation="MMVDTRSRERRGRKKKGNSELRFIPLGGLGQIGKNMFVLEYGED
                     IMVVDCGLMFPDEEMLGIDFVIPDASYLFENRDRIRGMVLTHGHEDHIGSLPLILPRL
                     DVPIYGTKLTLGMVDAKFRDSCPDYTPKSFEFSAGDKFSLGCFDITFIAVCHSIPDGV
                     GLAIETPVGTVVHTGDFKLDPTPVDGRITDYSTFSEFGSKGVLLMLSDSTNVERPGFT
                     QSERIIGRTLERLLRLHRNNRLVIAAFSTNLHRSQQILQVASRFNRKVALMGRSMIQN
                     VTLAIELGYIDVDKNILVPMEDVGKVPDNRLIVLTTGSQGEPFSGLVRMSKGEHRFVN
                     LGQGDVVAVFASPIPGNEKLVSRTINRLFECGCEVIYDRDQEVHVSGHAAREELKMML
                     SMVRPKYFVPMHGEYRHLVRHARLAQEMDIPARNTFVLQNGDLLSINGTKAQVKRGEI
                     TSGAILVDGMALGEMQDSLMRERQEISEEGLVVISITLDRDQRLIAPPLIDSVGFIHM
                     NDAGGLAKDLQDAVMESVAQLHEGKTQDIDKVSNRVRNRARSVLKRFSRTPPVIKIMI
                     TRAGS"
     gene            19207..20826
                     /locus_tag="P5541_00095"
     CDS             19207..20826
                     /locus_tag="P5541_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRV70404.1"
                     /translation="MSTGLFFNLLGGVGLFLFGIKLMGESLQDLAGDRMRRLISALTS
                     SPVKGILLGAIVTVLIQSSSATTVMTASFVHAGLMTLKQSIGVIMGASIGTTVTAQII
                     AFKIKEFALPIIGFGMIFAVFGRTKRHKYIGNGIVGFGLLFVGMQTMESAMYFLRDRQ
                     DIFLAFQHHPILGVLAGTLLTMLIQSSSATVGLTIVMASQGLLTLDSAIPILFGDNIG
                     TTITAVLASIGLNRSAKQSAFAHVFFKVIGVIVFLLFLEPFRHLVTLTSSDIARQLAN
                     AHTIFNLLNTLMFLPFINPFVRLVRTVLPDDGVLFMMGPRFLDKNLISASPIAAVEAV
                     RKELVRMGFLTLEMIRHVKKAFVDSDRKMIEQVNQTERCVNDLTREITAYSADLMQVS
                     LPPVLSSSLSLFVNGVGDIERVGDHAENLIELFEYKIEHNLPFSDLAMEEFNAMISMA
                     EEALLLAVEALETSDGDKVKKVFRLEDKIDAMERGLRKRHIERLNQGVCNPGSGVIFI
                     DILSNLERVGDHANNIALIVKDINLVRGKEE"
     gene            20828..21637
                     /locus_tag="P5541_00100"
     CDS             20828..21637
                     /locus_tag="P5541_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRV70405.1"
                     /translation="MLISQCALLGLLQGFTEFLPVSSSGHLVIAQSLFGWQDPAIAFD
                     VMLHLATMAATLVFFRKEIFYLALGWFGGLVSRAGRDHPGWRYGWSVLAGSVATVAIV
                     LVTRPVIHVFFGNTLFVGLALVFTGAVLWYGNGISVKDGEVSVPTGLMVGLAQGLAAF
                     PGLSRSGLTIIAGLKSGLGREEAFSFSFLLSLPAILGAAILELPDAGGISGVSGSLPQ
                     GWWIGVLLAFLSGLFALSLLRKVVVRGKWKVFAVYCLIAGAFVLAVNIFGR"
     gene            21643..21888
                     /locus_tag="P5541_00105"
     CDS             21643..21888
                     /locus_tag="P5541_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70406.1"
                     /translation="MARSGGSSRWWILILCVILGSFLGVYLQGFQATENLFRNVVNIG
                     FDIGDVDVLIARFGFRLFFRLNLGTFIGGVIGVLMIR"
     gene            21885..22487
                     /locus_tag="P5541_00110"
     CDS             21885..22487
                     /locus_tag="P5541_00110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRV70407.1"
                     /translation="MMIDQSFPQDVVLASASPRRAALLGDLGWSFRIVPPDIDEVVSG
                     GESPSGLVVRLADEKAECVCRKCPDALVISADTVVVLGDLILGKPENAQDAARMLSML
                     SGKRHTVMTGVALRHRCVSVSGFESTDVFFRELSEQEIERYVRSGESLDKAGAYAIQG
                     RGSLLVSSIEGCYFNVVGLPLFRLSRLLEEAGISLEQQWR"
     gene            22492..24399
                     /locus_tag="P5541_00115"
     CDS             22492..24399
                     /locus_tag="P5541_00115"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HRV70408.1"
                     /translation="MRVRQSFVLAAGMMVVAIIGALGLVPRISAERSGSVAALVVEMR
                     DVANMAREAGLPVEQAVERLEAEGLTALAVGELTGQELLSGMLPVEFDSARNLIHGDL
                     PEGVWPDSAALLFSEKDKLDDRIRFFVQTRFPGVIEVDKDEDLLLVLPFSLSETLEAG
                     ILPDYPLLEMASGTGMPLIYRPGSTPGISGDSVADSVEIVLDGFPNIRSVVPAGLFVA
                     GYPELGPLSRLLGEREISVAKVEFSRQIGVGFLERALFPNVLSLHSVRKEEIVSRRLT
                     RDDLVERFVRAARERSVQLLYLRPSDLLSGSRLDRFANECGRISGRLEKLGIGNGWPE
                     TLPLWRSGLFSAFACALALVLLSMRLVSRYLGAERDFGTKTLVALLGLSVVLAFLMLK
                     ISPVIKLSGALLAALAATEASLLALENFRKPVRGVLMSVGVAVASGLAIAGFFGTPWY
                     MLRMDAFSGVKLTLLLPPLLVLLHDLKRRVHPESLENVLSRPPHWGELALIGGILAAA
                     GLMVFRSGNVSFVPGWEIRFRELLEQILVARPRTKEIFAGYPALVLWFTVRRMDIWPG
                     YREIFRLGTTLAFSSVVNSFCHFHTHTTFIFLRVFNGLWTGLLVGLVLSAILVFLVLP
                     VWRRWREVLAG"
     gene            24396..25412
                     /locus_tag="P5541_00120"
     CDS             24396..25412
                     /locus_tag="P5541_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRV70409.1"
                     /translation="MKRPLDVTICGFYGFGNLGDELIAHGLVSLLIKNGIPSDRIAVL
                     SASGNIEGSYGTVLVPRWDPLKVFKTLGQSRTLLLGGGGLFQDSTSIRSCIYYWGIVR
                     MAVLAGCIPWAFGQSIGPLISGAASFFARDALSLCRVRCVRDEGSLDWLKANGLEGNI
                     APDTVLTLSDRMDINGGGRDHLLVNIRPWPGSLPATAASAARILGERTGLPLMGVALS
                     DDDLEEMKRLSGDGLFSPVAIRRVRTFDDVVETWRFGSEAFGMRLHFCVLSAMAGMPC
                     VAVPYDPKVSGFASRWGLPVWSGEGPLPEMVMCSGTKEKVTESSSMIEESFAKMLGTV
                     LGGE"
     gene            25418..26281
                     /locus_tag="P5541_00125"
     CDS             25418..26281
                     /locus_tag="P5541_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV70410.1"
                     /translation="MDISERRNLERKAVEARKDVVRMTGVARSGYLAASLSVVDILVF
                     LYWKEMNIRPAEPLWHGRDRFVPGKPHACPALYSVLANRGFFDRKELWNFRRLGSMLQ
                     GYPETPRTPGIDASGGSLGMSLGISNGMALALRLDGSEQRVFCLMGDVELQEGSVWES
                     VMQTANLGLDKVTVIVDMNSSGMHQGEDSVKEIEPVRNRFESFGWRVEETDGHDMDGL
                     ESAFAGTLAKNSTEGIPCAVLAHTIPGKGFSLAEKGCIGPLEVLDRNMMEQALREMES
                     SSAVFGRGEDH"
     gene            26278..27216
                     /locus_tag="P5541_00130"
     CDS             26278..27216
                     /locus_tag="P5541_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV70411.1"
                     /translation="MTSTLEGFAEGLVDFAEKNREVVLLHTDTAASCGVSIFEEKFPE
                     RSFNCGVSEQDMILTAAGLSLTGKIPFACSRAASMAPRAFDQIRASLSIPGLSAVLAG
                     TFSGLSAGEDGASQQMNQDIAIMRSLPQMTVLVPADASSAFSMVSLGALNQGPVYIRL
                     GSEKVEAVEAMAGSEMKIGGGRLLREGDGVTICACGIMVHEALKAADILFRQGINAEV
                     LDCFSLKPLPAPLILASLRRTGCCVTAEEHNEVGGLGEAVSALCAKKNPVPVRSVAIG
                     DKYGQSGKPPELQEYYGLTFREIVGEAAQVWSMRRR"
     gene            27220..27915
                     /locus_tag="P5541_00135"
     CDS             27220..27915
                     /locus_tag="P5541_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV70412.1"
                     /translation="MEIRFAGVTKQFTPDIVALKDVFLTIAKGEFVYLVGPTGSGKTT
                     LLRLVTRELIPTKGQVTVGDTNIRKIASYDLAYLRRDIGVVFQDFRLLPHLTVYENVA
                     FVQEAIGVPPSDVQKRTNDVLDRLGLWRRRSLFPEQISGGEQQRVAIGRAIANSPSIL
                     LADEPTGNLDIHKAEEITQLLLSINASGTTVIVATHNQYIVDAYRQRVVELRKGLVKR
                     DERKGRYEVDGDL"
     gene            27902..28789
                     /locus_tag="P5541_00140"
     CDS             27902..28789
                     /locus_tag="P5541_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRV70413.1"
                     /translation="MATFNYILRDTWRLLSRHWGLSVLTLLTSVAVFFLVGASVLFVL
                     NTRYLVAAVEGDLMVQAYVKNTPEALESVARKASSYDSVSSVNIITSDEALEKLKAKL
                     GKQAEAVTLLDENPLPPSVEVKVKRAAFASVIARELISMPEVEDVVYSGAIADRLERI
                     SSFVSKLSVIILAVSMASASLVLFNTIRISVYARKEEISTMLLVGATRNFVVFPYVLQ
                     GVVLGTLGALLSALLIWFSYGAAIEALERSLPFLRLISGTGLVLRLGLVLVCAGVTVG
                     WMCSWLAASRFIRLASRSH"
     gene            28812..29993
                     /locus_tag="P5541_00145"
     CDS             28812..29993
                     /locus_tag="P5541_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRV70414.1"
                     /translation="MKLNSRFLTLLILLSVFSLYSGVAVASEIDSKIRTEQNRLEAIE
                     KQIAYHQRQIADSRKKEQGLLDELSKINQNITLLKQKVAVLDLQREKTEIRIGELDSE
                     IEKTVSELEKAKRYLAGRFQALYKYGGVAEFNLLLSAASAHEAMNNAFLLGRIAREDE
                     RIIRETSESRKRLEAARLEMGEQKTLLDNQRKLLGEQQSSLKNSETQRKKSLDTLFRQ
                     KQLHEKAARELEESQKELQNKVRELLAQKRKMQETRAGVVTPVFPTGGKLAWPVRGDV
                     TSKFGSRVHPVFKTKMMHTGVDIRAGEGTPVKTAAPGEVLFAGWLRGYGQVIIIDHGR
                     DLTTVYAHLSQIRVEEGKIVKNGEIIGNVGSTGVTSGPHLHFEVRVNGDAKDPLGYLA
                     R"
     gene            30025..31206
                     /locus_tag="P5541_00150"
     CDS             30025..31206
                     /locus_tag="P5541_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRV70415.1"
                     /translation="MIRRVRDILFGAITGAILAGWVFSAGAGSGGDVFSASPFGFQDL
                     WLMKQARVIVETYHVEGLKDVDERVLLYGAIRGMVAALDDPYTRFVDPDELHEEEIEM
                     QGEYGGLGIYIGQRDGRTLVISPIEDTPADRVGLKPQDQIVKIGEEVILGWTQNEVVN
                     NLRGEPGTGVTIWIRREGVEDLLRFDIVREKISIQTVKSEMLPDKVGLLRIIQFNQKT
                     VPDFGTALKKLEEQGARGFVLDLRNNPGGLLNSCVDIADMFLDNGVIVSTRGRFDRAN
                     EVLFARPGMMTRKPVVVLVNEGSASASEILAGALKDQGRAQVVGKKTYGKGSVQTLFN
                     LSDDSGVFVTIARYFTPSGTMIDKVGLSPDIEVEGGPEKEREKDEQLNRAIKELLLIA
                     R"
     gene            31238..32002
                     /locus_tag="P5541_00155"
     CDS             31238..32002
                     /locus_tag="P5541_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRV70416.1"
                     /translation="MKRGFILLIVTMGVFSHMPLSNGWCDVSRPVIAVIVDDLGYSVE
                     MARDLARIPLPQTWAIIPYLAESRRTSEIALDKDIPFMLHLPMEAVGDDGKRHSLVWV
                     GMSEESIRQAVRNALWSLPGVSGLNNHRGSKASQDRGVMGAVLREIAAEGLFFVDSRT
                     SGDSVGFPVAVEMGVPATLNRSFMDHEDNEGFMWSQFRKAVGIARKTGGAVIICHARP
                     GTLKFLPRLYEKAHEDVKFVTVPEYLEQKRTGGWEE"
     gene            32007..33293
                     /locus_tag="P5541_00160"
     CDS             32007..33293
                     /locus_tag="P5541_00160"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRV70417.1"
                     /translation="MKGRVEAIIGAQWGDEGKGRVVDALASRVDVVARYQGGANAGHT
                     VIVEGEKYVFHLLPSGMLYSGKTCVIGNGVVVDPEQLLKELKELQDRGKDRARLVISG
                     AAHVVMPYHKTMDGMEERLRGSGKKIGTTQRGIGPCYMDKVNRCGIRVEDLLSEDVLR
                     EKLSFNMEIKNMYLTRIFDENPMAFGEVFEVARGWGEKLAPYVGDASLTISNAVESGS
                     NVLLEGAQGTLLDLDHGTYPFVTSSSPVAGGGSVGCGLGPSMIHRVTGVVKAYCTRVG
                     EGPFPTEDTDVDGETLRNRGGEYGATTGRPRRCGWLDLVALKYAVRVNGLHAIALTKL
                     DVLSGFDKIPVCTAYETGGDETIHFSGNTGFLDSVRPVFKYLDGWKKDLGKSRKMEDL
                     PAAARNYVSFIEEETGVPVVLVGVGPSREQSIVQGL"
     gene            complement(33369..33443)
                     /locus_tag="P5541_00165"
     tRNA            complement(33369..33443)
                     /locus_tag="P5541_00165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33409..33411),aa:Val,seq:cac)
     gene            33720..34259
                     /locus_tag="P5541_00170"
     CDS             33720..34259
                     /locus_tag="P5541_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70418.1"
                     /translation="MKDILNDPRMLEIFWSEYGFEEWSGLGYRGVSRRVTFRKSSLMG
                     EVARYYSDDYILRAPDAGAAARHILDNWQPVKDVMSHRILVLGDHGLEKMFQRSFFFG
                     FKGWVEVSGYRPGDPLPNRKFADLCTLANNAGVVLDQILEGINPVKERIIDPGKRGVF
                     AGKGEKGDPSGFIKKLFRR"
     gene            34266..35870
                     /locus_tag="P5541_00175"
     CDS             34266..35870
                     /locus_tag="P5541_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRV70419.1"
                     /translation="MLAMLFIFASVFFVGAYFTYGNFQAKLYGLSNDNKPPSEVYFDG
                     VDYVPAHPSVLLGHHFASIAGAGPIVGPITAAALFGWLPTYIWCLVGSVFFGGVHDMG
                     ALVSSMRHKGLSVGEVVDKWIGRRGKMLFLTFTWLALVLVVAVFLQLSANTFAADPAV
                     AFSGTLYIFLAVVFGVLIYKKGIPLGVTTLVMVPIVIGAVFYGSSAEWVQTSFNYDVN
                     TWRLILTAYVFLASVLPVWLLLQPRDYLASFMLYFSVLLGAIGMIFGTKFSTDLPAFK
                     GFYAGGTNYLWPMLFVIVACGAISGFHALVGSGTTSKQLKSEKDAQLVGYGSMLIEGL
                     VAVIAIGTVMVAGGIMEGGPTVTYGHGIGQFAGLIGIDIKVGTSLGLLALNSFLLTSL
                     DTATRLARYQLQEFTGMKLNKYVATAISVFGALFLLYYKTGGKAAWALIWPVFGASNQ
                     LVAALTLLALAVWVKNGLKKDNRFAMYPFFFMLATTMAALFFLIKTNLNNLLLSGISV
                     VLVVLAVMLVMEAKKALSGNGPKPQE"
     gene            complement(35990..36163)
                     /locus_tag="P5541_00180"
     CDS             complement(35990..36163)
                     /locus_tag="P5541_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypA/HybF"
                     /protein_id="HRV70420.1"
                     /translation="MRKYRCLHCGHEFVVEDTAPGLKCPMCYNRFVELIEGSRVKGKS
                     WGSKSFSVPKIGG"
     gene            complement(36258..36938)
                     /locus_tag="P5541_00185"
     CDS             complement(36258..36938)
                     /locus_tag="P5541_00185"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRV70421.1"
                     /translation="MTGMTLLAALWLGILASVSPCPLAANMAAFSFIQKDVTTSNRIM
                     LSGILYSAGRMVAFATAGLLVISGILAIPEASIFMQKHMNRIIGPILLIVGVFVLDLL
                     EIRSGKGISRSLTLTGMFRGSGGAFLMGILFAMAMCPVSAALFFGGLVPLAAEAGSKI
                     LLPALFGLGSALPVAILSFVFSLGTRAVSKSMGAVYTIQKMVQTVSGIVLLLVGVYFS
                     LTSTFPIF"
     gene            complement(36935..37420)
                     /locus_tag="P5541_00190"
     CDS             complement(36935..37420)
                     /locus_tag="P5541_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70422.1"
                     /translation="MNPKKALGTIIILFALLGLARAAYEIHRDMAVRADSLSIKAPQG
                     SVPKSEVEYTIYFFEGNTPCPVCEKIRAMTLNLKSSGNPSISRFSIKEINVDEPGNEI
                     YILDLGLFSTSVVLAKESDGLIYRWKNLEAVWDLSGDEDSFRKYLIEESDSFLEGADT
                     R"
     gene            37528..39705
                     /locus_tag="P5541_00195"
     CDS             37528..39705
                     /locus_tag="P5541_00195"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRV70423.1"
                     /translation="MKSDDLAQEKRIKTAVRITGMTCAACSNAVERALRKVEGVDFAA
                     VNLSTETAFIIHSPETNRLDLEKAVEKVGYGVTDEMPEALERKRYLDSRRNLVEALAV
                     TVPLAVLMIMHMAGYHMAWFPMMELVAGGFVVFRSGRNNIRGAWIALTHLHTNMDTLI
                     SIGSIAAWLTSLLHVTGVPVASFGSIGAMIVALHILGRFIESSLRDRAAKEIKNLLKL
                     RAREARVDVNGKEMTIPIEAVKSGMTVRVSPGGLIPVDGDVISGRSSVDESMITGESV
                     PVMKEKGASVTGGSMNLTGEMFVSVTRTGEDTFLSKMVSLVQEAQGSRIPIQALADAV
                     TRVFVPVVTILALISGFTWFVFFRELYPGLETLRHWFPWILETDSPASFGVFVFVATM
                     VIACPCALGLATPMALVAGTGMATRRGLLIRNAEAIQTTRDIGTVIMDKTGTLTEGDP
                     KVVFHDLSREVLAAVAAIERRSDHPLARAIGGAFEGTLPVPSDIVEIAGKGVRGLVEG
                     SVYEIGKPPLPEAYMEYLERGATVVEVNRDGKPSGFLVIEDPLREDSVKAVEEMKRMG
                     ITPVMLTGDNEVTARSVAVKTGISEIYAGVMPEGKLEIVRRYQSEGAKVLMIGDGIND
                     AAALKGADIGMAIGSGMDLAIDSADVVVMSGGISRAVECIRISGKTFRVIRQNLFWAF
                     LYNLVAIPVAMGGLLHPAIAEGAMAMSSISVVLNSLRIRKEEH"
     gene            39705..39905
                     /locus_tag="P5541_00200"
     CDS             39705..39905
                     /locus_tag="P5541_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRV70424.1"
                     /translation="MGERLVYGVPAMSCGHCVRRISEVLVSLGVEGFDVSLENKVIYT
                     DDPLDEKVIKALSDAGYEVFVR"
     gene            40047..41783
                     /locus_tag="P5541_00205"
     CDS             40047..41783
                     /locus_tag="P5541_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRV70425.1"
                     /translation="MPQFPHEGFSDLSIVLCGAAGQGVQTVEEILVDSLRRSGLNLFA
                     SREYMSRVRGGSNSTELRVSSERVNALVDRIDILLPLNGGIRPNIRKRISPGTLILGD
                     IEELKDEFGDSPGIFMNVPLMEKGRESGGAVYTGVVAVGLLSGLLDLPTGIIRSIIEE
                     KFSGKSAEIVRNNLSALEKGLEIASELRSSGDIVVEIPNPSAGAKEMVLNGSDAVSLG
                     AIAGGCDFIASYPMSPATGVLTFMARHARKFGVAVEQAEDEIAAINMATGASYGGARS
                     MVSTSGGGLSLMSEGVSLAGITEIPVVLHIGQRPGPATGMATRTEQGDLNLAVFCGHG
                     DIPRIVFAPGTLEQAFRYTREAFNLAAEFQIPVFVLTDQYFLNSFYNIPPFDLQDFRV
                     ERHLVEAVPGYRRYENTPDGVSPRSVPGFGMELVGTDSHEHDAWGHAQEDFELRKSMV
                     EKRLRKMKGILGKAEKPTLEGPEDFRDLVVSWGSTCHIVREALEKTNSGKVAHLHFGQ
                     VFPLHRDTVDILASAERVVAVEGNATGQFAGLIRKETGFLIEEKVLWYSGLQMSVETV
                     AQGLKERLGQGG"
     gene            41786..42646
                     /locus_tag="P5541_00210"
     CDS             41786..42646
                     /locus_tag="P5541_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV70426.1"
                     /translation="MDHRIYDLEGIDNQWCPGCGDFQIMEALKKALSELDIPPEKVVL
                     FSGIGQAAKMPHFMRGHFFNGLHGRSISNATGMKAANRDLTVLAIGGDGDMYGEGGNH
                     FTHTIRRNPDITNLVHNNRVYGLTKGQASPTSPRGFVTPVQVDGVFPEPFNPVAIAVA
                     QGASFVARAFSGNVEQTVSIIKQAILHRGYSLVDIFQPCVSFNRVNTFAWYRENTYEI
                     PAGHDPGDRKAALDRAMEEAPYPLGVIFRDGDRKTFEDSLAVYEEDDRPLFMREYGPE
                     RIKDYMASLI"
     gene            42693..43037
                     /locus_tag="P5541_00215"
     CDS             42693..43037
                     /locus_tag="P5541_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70427.1"
                     /translation="MEFKKTAAAIGILLLLATTAVGHPPSSMEIEYSKDDGVLSIGIV
                     HPVGDVSTHFIESVTVSVDGNTVADLVYLEQWDKGGEKIVVTIGRFGPGSKISVKAVC
                     NKIGTLEKTLDL"
     gene            complement(43098..43847)
                     /locus_tag="P5541_00220"
     CDS             complement(43098..43847)
                     /locus_tag="P5541_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70428.1"
                     /translation="MISRMKKTASLLSVMFAIAVFFPASSHAQYLKILSVPGHPVNLV
                     LEVSEGVITSALLRSPAGIQKILPIEGYVYSGENVVEPYADGDFRKDLLWSITLTRPG
                     DRSRGIYLWIGVTTNTPRAWVIISPLGQTYWDSIPVKVYAPRGIGLFVSPNLPAYKDL
                     PQFGGSRTLTFVYTIALTGEGPNFQVIPEVYRQLHKITAMVRDAEQIPERKEAYSLLL
                     SDYESLSRGGKPSTEVIQNFTWKRILNLDWK"
     gene            complement(43876..44358)
                     /gene="folK"
                     /locus_tag="P5541_00225"
     CDS             complement(43876..44358)
                     /gene="folK"
                     /locus_tag="P5541_00225"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRV70429.1"
                     /translation="MRAGLSLGSNLGDRLSCLRQALNLLREKGLRILALSDVYETPPW
                     GVVDQPAFLNACILVESDDEPGEMLEKLKSIEDEMGRIDRFRWGPREIDLDILFMDDI
                     AVNEKDLVIPHQEMHRRAFVLVPLMQIAPDWVHPLLGKSVKELAEQVEHKNIEKITSL
                     "
     gene            complement(44358..45572)
                     /gene="folP"
                     /locus_tag="P5541_00230"
     CDS             complement(44358..45572)
                     /gene="folP"
                     /locus_tag="P5541_00230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRV70430.1"
                     /translation="MTVYRFHLQNDHDLRKALSLTGADVRSMPYFDQKREILAFHLAD
                     IDFRAANALKQEMLSRDGDAIVHRGAIDGSVARCDVILMGTRKVFSSLLEKLGAMPYW
                     GLDVVREELEAATGAANIRKWEIPLPGRPPLDLGGRTAIMGIINVNDSSFYSGSRAST
                     TEACADMAEKMDMEGADIIDLGAESTRPGSTPLSGPEERDRLIPAIKAVRERVPEALI
                     SADTYRADTASEAIEAGADIINDISGGIFDPLMFPLLARTGNPVVIMHAMEVPDGMHK
                     KYAYRDLIGEITAFFFDRIRSAAEAGISPQSVILDPGIGFSKNSSQSLEVIAQIDSFS
                     TLGRPILVGHSRKSSIGRVLELDKPSERLEGTLAISAFCAMKEVPLVRVHDVGQNLRA
                     VKMIEALKGGKA"
     gene            complement(45620..46267)
                     /locus_tag="P5541_00235"
     CDS             complement(45620..46267)
                     /locus_tag="P5541_00235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV70431.1"
                     /translation="MSNGKRRTTIVLVRHGECEGNREGLFRGRTDFPLNGSGLEQARA
                     LAKVLSSQDISVVYSSPLARSIETARAIGEEGGVPVEIRQGFNNMALGKWENRKKKEI
                     EQEYPEEWRLWLSHPERLHISGAETLSDVQRRAFANLDNLVNIHEGETFTIVSHRAVL
                     KPLLAAAINIRDPYFWRIHIDTASFSVMTFEKERGYCLVSLNQTSHLEHIISEWV"
     gene            46461..47480
                     /gene="hrcA"
                     /locus_tag="P5541_00240"
     CDS             46461..47480
                     /gene="hrcA"
                     /locus_tag="P5541_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRV70432.1"
                     /translation="MLSERHLEVVLAVVYEYIKTGEPIGSRTISRKYLRDRSAATIRN
                     EMADLEEMEYLYQPHTSAGRIPTPRAYRVYVDAILRRQRSFPLEDDLWVRELRARRKD
                     AEEALVYATRLLGKITSYIGVAALGPVSETVLQRVEFVRMGHRTLLILLVLKGGLIHH
                     KTVTLPCDMTQEALDELSRRVSVVAVGRSWKDVRGVLQSYVEDALEKYAENCRSAISE
                     MDGIMMDQTFQFFTGGTHNVLGMPDFSDLGRLQAIMALLEEGESMSKLVNDCSAEQGL
                     CITIGDENPVFQMRDCSIVMASAKTGGRKAVVGLIGPVRMDYERSISVLEGILDTLSA
                     DVEVE"
     gene            47493..48101
                     /locus_tag="P5541_00245"
     CDS             47493..48101
                     /locus_tag="P5541_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRV70433.1"
                     /translation="MKMAEDGKFSEKEQEEALGPTGDEIEEVVGHDLQDGEENVDELR
                     ASRELAEKLMEENEKLKDAAARTRADFFNYRQRVERDRERLARSASEDAAEALIPVLD
                     NLERALSSPSGDFDSLFKGVEMVRNQFFSVLEGLGLSRIETGGQPFDPAFHEAVAIEE
                     IEDESLDGVITEELQPGYTIAGKVLRAARVRVARAIQRGTEQ"
     gene            48125..49951
                     /gene="dnaK"
                     /locus_tag="P5541_00250"
     CDS             48125..49951
                     /gene="dnaK"
                     /locus_tag="P5541_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV70434.1"
                     /translation="MSKVIGIDLGTTNSCVAVKEGDHVTVITNAEGARTTPSVVAFTK
                     EKERLVGQLAKRQAIVNPERTISSIKRHMGSDHKVEVDNTAHNPQQISAMILQKLKRD
                     AEEYLGEKVGQAVITVPAYFTDAQRQATKDAGRIAGLEVLRIINEPTAAALAYGTDKG
                     GDNKILVFDLGGGTFDVSVLDVGIEDGVIEVLATSGDNLLGGDDWDQKIMDLMVSEFK
                     KSEGIDLSKDKMALQRLREAAEKAKVELSTMSETSISLPFITADASGPKHFEMTLTRA
                     RFEEITRSLLDRVVQPTKRALNDSGLTREEISKVLLVGGATRMPMVQKKIVELLGKEP
                     TKGINPDECVAVGAAIQGAILTGEHKDIILVDVTPLSLGIETLGGVFTKIIDRNTAIP
                     VSKSQVFSTASDNQPQVEIHVLQGERSMAGDNVTLGKFFLDGIPPAPRGIPQIEVTFD
                     IDVNGILNVSAKDKGTSREQHVTIQSSRLAEDEIEKMTREAEENQESDKKKKELVDAR
                     NEADSLIYSTEKMISDLGEKVTEEEKTRISGEIEDVKKALGEENPEGIRQVAEKLTKS
                     LHEMSSRLYAQAGSEDASDGTEPEGSDGEETVDAEFEDNGKV"
     gene            49965..51083
                     /gene="dnaJ"
                     /locus_tag="P5541_00255"
     CDS             49965..51083
                     /gene="dnaJ"
                     /locus_tag="P5541_00255"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRV70435.1"
                     /translation="MPAGDERDLYAILGVSRNATQEELKKAYRKLVRKHHPDANPGDP
                     EAESKFKSINLAYEILGDPQKRAAYDQYGTIGDTPGGSPFGGGFEPFGDIFGDLFGSF
                     FGGGRRGPAGPSRGDDLEMSIKISLEDAFRGLGREVVIPRMDTCGRCGGSGAEPGTSV
                     KTCSHCGGSGQVEKQQRTPFGTFVSVGQCPHCGGSGKRIESRCKNCGGSGKVRSDHRV
                     EVKIPPGVDTGTRLRIAGEGREGSSGGPPGDLFISIKVEPHPLFERDRDDLHTRVNLK
                     YPQMVLGCSVEIPGIEGGEKLEVSPGTPVGKAVRIKNKGMPRLRGSGRGDLFAHVYVD
                     IPTELSEKERLLTEALAQEMDVGVEPAGFMEKIRNLFG"
     gene            51185..52054
                     /locus_tag="P5541_00260"
     CDS             51185..52054
                     /locus_tag="P5541_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRV70436.1"
                     /translation="MSSNEGFWWYITIEGDSESEEDLVSLGELSGSIGSEMQELPGKV
                     ALKAYYRNTRDLGSWMERVQEVISPWPGVRIADMGRIENRQWHTAWKEAFPPLPVGDN
                     FIVMAPWHRGNEPEGKIPVYIYPGSAFGTGYHESTQIALELLEKTVKPGLSVADIGTG
                     SGILSIASALLGAGQVLARDNDPAIINEVRENLRLNDISPEKVLVQTGDLLAGVSGKY
                     GIICANIVFDPLASMLPSVPSLLEEGGVAVFSGLTLKERDPFLVEVSSAGLSPVTELT
                     KGDWWGVAASRTP"
     gene            52029..52751
                     /locus_tag="P5541_00265"
     CDS             52029..52751
                     /locus_tag="P5541_00265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRV70437.1"
                     /translation="MSLPRVRLEHSVQESDSVWVLDGEQGRHLLTVRRCRDGEFFEGL
                     LPGRRILLRLETCGTLARGVKVRETPEEPMPEIWLLAGLLKGDSFDRLLSQSVEVGAS
                     VIVPLVCTRSVPILEPSKIARKMERWKKITLEASKQCCAPTPTRILDPVGISSLGSLP
                     VPSARFVAVAGASSKISEFSPPGTAAVATGPEGDWTEEEIHILQQHDFIPVNLGLRVM
                     RAHTAAVVAVAFLALLSGGQKL"
     gene            52748..54061
                     /locus_tag="P5541_00270"
     CDS             52748..54061
                     /locus_tag="P5541_00270"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRV70438.1"
                     /translation="MKDPLRGVKFRVIPMGCRTNLFECEALCCSFVESGAARSETKDF
                     DVAVLVGCSVTSEADRKCRQILRRLRREKKDALIIAAGCWAQKATIEEAAGLGVDCLV
                     GNRLKHRIPAIVREMLLSGRRTEQPRIEMVDVSNFSGWEDLRLLRPQTRTRAFVKIQD
                     GCDHGCSYCIIPALRGKGVERDPLDVLQEVERIALSGCREVVFTGINLGVYGRNSGMP
                     LGELVKRSGDIEGIARIRFGSLEPFAVTETLLGDLSATAQFCPHFHLPLQSGDPDILR
                     LMRRGYTPEDFLAHVEMARSFFGEDLHISTDIIVGFPGESEDAFDRTLSLLVSAGTGR
                     LHVFPFSSRDGTVAASIPGRLKGPEIRSRVERSIALGKDLLDSYSGKWIGKRVDLLVE
                     GRTGGMMEGFSSHYVRVECPGDSEPGMIRSVFVTDSLDGVLRAVV"
     gene            54137..54487
                     /locus_tag="P5541_00275"
     CDS             54137..54487
                     /locus_tag="P5541_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRV70439.1"
                     /translation="MGNGSERCIFCRIARGLEKADVVYSDEDVIVIRDIHPIAPVHLL
                     VIPRIHVESADKVADPSVWSSVMGAAVRVARDMGLGPDGYRLVVNCGAGAGQTVSHLH
                     VHLLAGRSFGWPPG"
     gene            54520..54696
                     /gene="rpsU"
                     /locus_tag="P5541_00280"
     CDS             54520..54696
                     /gene="rpsU"
                     /locus_tag="P5541_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRV70440.1"
                     /translation="MTQVVRRDNESLEDALKRFKREVSKGGVLREARRRSHYEKPSEA
                     KKRKREDASRKRRK"
     gene            54707..55165
                     /locus_tag="P5541_00285"
     CDS             54707..55165
                     /locus_tag="P5541_00285"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRV70441.1"
                     /translation="MTELEARIHQEIVSAMKRRDQSSLVPLRMLKAAVENARIEKGRG
                     EPFSEEDVFAVIRKLVKQRKESAGQFEAGGALERAGEELMEAELLSSFLPPSMSDEDI
                     IHEAGKTIAAVGAKGPSDLGLVMGPCMAALKGRAEGARVRKIVENILRSL"
     gene            55294..55764
                     /gene="nrdR"
                     /locus_tag="P5541_00290"
     CDS             55294..55764
                     /gene="nrdR"
                     /locus_tag="P5541_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRV70442.1"
                     /translation="MRCPKCGYMETRVLETRTASEGKVIRRRRECPECFNRFTTYERL
                     DERPVLWIIKKGGQREGFDRAKLLKGLAKACEKLPVPLEQIEEAASRIEETLREMGQG
                     EISSLQVGSLAMNELRNIHKVAYVRFASVYREFTDISSFVEEISRLHDERNGHS"
     gene            55761..57908
                     /gene="nrdD"
                     /locus_tag="P5541_00295"
     CDS             55761..57908
                     /gene="nrdD"
                     /locus_tag="P5541_00295"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRV70443.1"
                     /translation="MKDSDSRIMMEEKLSPGDFPGGRQMLIFSGKEESTDLALMVEAF
                     GQEDRSAWDVVRIRDALVVEAGVDPSVAGEIAAEVESDIMIHGRERVTTAIIREMVNV
                     KLFFRGLDAKLADHSRLGLPVFDLESMMLHPNRENSNTSHNPESINLSVAEMILKEYA
                     LGKIFSREVAEAHLGGDIHLHDLGMVNRPYCSGQSMAYVIKYGIDIPSITSVSSPARH
                     PDVLLAHILKMTSVLQNNFAGAIGWDAVNVFFAPFLEGLPYEKVKQLAQMLIFEFNQL
                     AGGRGGQVAFTDINLYYEIPDHFRDVPALGPGGVPTGRTYYDYRKESKDFLRALFEVY
                     LEGDSRGQPFFFPKPLLHITDRFFEEPGWEKFLELACLVASEKGSTYFVFDRGGVAKL
                     SECCRLSFELSEEDLAEAKTPWKMRYCALQNVTINLPRIAYRSGRDRETLFELLDRAM
                     ETAAEAHVQKRRFLQEILALGSKGPLSVLCVDHDGESYLRMSKSSHLIGILGLDEMIH
                     SFTGTRMHMSDEAMDLGLAVTRYMDLKCRQLSDRLGLKIVLEQTPAESTAYRLARLDL
                     KFFPDDARHFVKGDISSGEIYYTNSTHFAYDADLDPVSRVIMEGRFHPMINAGAISHV
                     WMGEHRPDPGALASFVRKAYESSENAQIAFSPEFTICNKCGKMERGLLDSCPACGSMD
                     VDGITRITGYFTRTSSWNAGKRAELRDRSRTAV"
     gene            57912..58583
                     /locus_tag="P5541_00300"
     CDS             57912..58583
                     /locus_tag="P5541_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRV70444.1"
                     /translation="MKPGVPIGGFLPVSFVDWAGHVASVLFLCGCNFRCPYCHNADLV
                     FATKGSIDADDVVKKILSRKDFLDGIVISGGEPTIHSGLPLFLSFLKGETGLKIKVDT
                     NGSNPGMLETIIAEGLVEGLSMDIKAPWNDYPRITGSGSDEVAKSLDLVRRSGIPHET
                     RTTFVPDIMSIEDLKEIRDQVGRGPGWVIQLFRPGETLDLSLREGTVPDRGLLESNFP
                     GVLVR"
     gene            58804..60000
                     /locus_tag="P5541_00305"
     CDS             58804..60000
                     /locus_tag="P5541_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV70445.1"
                     /translation="MKKGLLLGLIVVIAVFGFMMTRGEKAPEAPAPEQTQEAQEPVAE
                     ADVIRIGVYLPLTGQNAFGGQLELDGVKMAHEEKPEVLGRKVELFVVDNKSDKVESAN
                     AVKRLIDKENVVAVIGTYGSSLAMAGGEVAEKAGIPMVGTSCTNPLVTQGKKYIFRVC
                     FIDPLQGAGAATYAHKVLGARKAALLTDVANDYAVGLAGFFEKSFVKLGGEIVSKLNY
                     NSGDQDFTAQLTEIISKGPDILFIPAYFAEGAIIMKQAQELGVEFQIMGGDAMDNPQI
                     VEIGGDAVEGFVHTTFPYDPSMQDMNPVAQKFTDDWKAKRTDDPNVNAALGYDSYMIL
                     IDAIERAGSADPEAITAALAATKDFEGVTGLTTINETHDAEKPIGIVQIKDGKKQYIA
                     TIAPEL"
     gene            60116..61009
                     /locus_tag="P5541_00310"
     CDS             60116..61009
                     /locus_tag="P5541_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV70446.1"
                     /translation="MSLAMFVQHLFNALTLGSLYALIAIGYTMVYGILRLINFAHGEI
                     FMLGAYFVFWGVTLFHLPWIVSVIGAIAVTSFAGILVDRVAYKPLREAPRISALISSI
                     GVSFFLQNFAIVVFSAIPRQVQRPDWLVKVIIVGKIRILPLTLVVPVLSFVLMLGLLY
                     IVYKTKPGLGMRAISKDIETSRLMGVPVNRIIAITFGIGSALAAASGIMWALRYPQLQ
                     PVMGVIPGFKAFIAAVFGGIGSIQGAMIGGLILGFIEIMVVAFLPALAGFRDAFAFIL
                     LIIILLIMPTGLMGEKLEDKV"
     gene            61009..62055
                     /locus_tag="P5541_00315"
     CDS             61009..62055
                     /locus_tag="P5541_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV70447.1"
                     /translation="MDARMKRNYTLNGVAVMLLGIFLFWASGSLDGYKIQILNLIAVN
                     IILALSLNLIYGFTGLFSLGHAGFMAIGAYTCSLLILPAAHKQAMFLLQSPMWPFSVM
                     HAPFIVAIVIAGLVAAFFGVLIGLPVLRLGGDYLGIATLGFAEIIRVLANNMPRVTNG
                     ALGLKGIPSYSNLWWNYGWCLITLFVIMRLVRSNFGNTLKAIRDDEVAARAMGIDTFR
                     YRLTSFTIGAFFAGVGGALLASLLTTIDPKMFLFILTFNVLMIVVAGGLGSLTGTVLA
                     GVVITVLLEWLRIVENPFSVGSITVPAIPGMRMVVFSLALLFIILFRQQGIMGKREFS
                     WDWFFGLFGKRGDA"
     gene            62055..62843
                     /locus_tag="P5541_00320"
     CDS             62055..62843
                     /locus_tag="P5541_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70448.1"
                     /translation="MSERRIVLETKNLTMRFGGLTAVREFDIQVQKGRIVGLIGPNGA
                     GKTTCFNMITGFYKPTEGQIFFEGTEITGWPPHKVCGGGIARTFQNIRLFHTASVLQN
                     VMVGARVRQRSTWWHALLNLPLYWKEEREVRDKAMFLLEAVGLQKLADEQSGALPYGA
                     QRRLEIARALATDPSFLLLDEPAAGMNPHETQELMGFIRDIRKRFDLTILLIEHDMKV
                     VMGICEHMWVLDYGVTIAEGQPAEIQSNTRVIEAYLGEEFVRDA"
     gene            62836..63540
                     /locus_tag="P5541_00325"
     CDS             62836..63540
                     /locus_tag="P5541_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70449.1"
                     /translation="MLRISDLNVHYGGIHAVKGISLEVPRGKIVTLIGANGAGKSSTL
                     RAIAGLIKKSRGSVLFGDIEIGGWVPEEIVKAGITMAPEGRRILPNLTVYENLQLGAF
                     SRSDTKGISRDLDWVFQLFPRLKERTWQKGGTLSGGEQQMLAVGRALMGAPQMLMLDE
                     PSLGLAPLLVKEVFSIIETINSEGKTVLLVEQNAYAALKVASRAYVLEVGSIVLSGTG
                     DELLNNPSVRDAYLGG"
     gene            63631..64173
                     /gene="pgsA"
                     /locus_tag="P5541_00330"
     CDS             63631..64173
                     /gene="pgsA"
                     /locus_tag="P5541_00330"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRV70450.1"
                     /translation="MTVWNVPNVLSLSRVFLAPLVMVFLTLRGQFGNIFGLPFGDILA
                     GLVFTIAALTDTVDGYIARKHGMVTNLGKLIDPLADKILVVAALISLVQLQRLPAWIV
                     VVIISREFFVTGLRMVAVSEGVVIPASRGGKAKTVTQIIAILMVIFNVPGGLLAMWVA
                     MIATLWSGMDYLLKGRKVLS"
     gene            64304..65698
                     /locus_tag="P5541_00335"
     CDS             64304..65698
                     /locus_tag="P5541_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRV70451.1"
                     /translation="MARPIQPEPFRIKMVEPVTVPDSGQREEALKRGHFNMFGLTSRD
                     IYIDLLTDSGTGAMSKQQWAALMRGDEAYAGADSFFALKDSVREVLGFDHVIPTHQGR
                     AAENVAFGTLVKKGDIIPFNMPFDTTRAHVFNMGAEPVNCVIDEAFDPSSLHPFKGDV
                     DIAKLEEVIKKYGKDRIPLIMITVTNNSGGGQPVSLSSLRAVSEVAKREGIPVFLDAA
                     RMAENAYFIKEREKGFAEMSVSRILREMMDTADAITVSCKKDPLVNIGGMICCRTEEM
                     YQKLLPRVILFEGFATYGGLAGRDLEALAVGLREMTDEQYMAHRIAQVRYLGDILDDG
                     GVPFVKPAGGHAIFIDAAAFLPHIPQKCYPADVLGIEIYREAAIRGIGLGALAFATED
                     ETTGEIVYPRLELYRLAINRRTYTNSHMEYVGESIVNVYRRRDSIRYGLRVAYAPPVK
                     GLHHFLAHLEPFSL"
     gene            65832..68498
                     /gene="secA"
                     /locus_tag="P5541_00340"
     CDS             65832..68498
                     /gene="secA"
                     /locus_tag="P5541_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRV70452.1"
                     /translation="MLEGLKKKLGLDHNERALKRYASVVSRINGLESSMSDLSDGELS
                     ALGKTFRERCAAGESLDDMLPEVFAAVREVSRRTLGMRHFDVQLMGGMALHEGKIAEM
                     KTGEGKTLVASLAVVLNAFTGRGVHLVTVNDYLAKRDAEWMGPIYRFLGLSVGVIYSF
                     MDQAERIKAYSADVTYGTNSEFGFDYLRDNMAVHSAQLVQREHSFIILDEVDSILIDE
                     ARTPLIISGPSEDNVEVYVRADEVARRLRKGEDFELEEKERNVALTEAGISRCEKILE
                     IPELFTDYNRSELGHRIIQSLKAHHLFQKDIHYVNKDGEIVIVDEFTGRLMFGRRYSD
                     GLHQAIEAKEKVQVGRENQTLATITLQNYVRMYRKVAGMTGTAATEWEEFKDIYGLEV
                     LVIPTHKPMIREDRPDVIFRTQNEKFAAAADEIEETFGTGQPVLVGTTSIESSERLGK
                     MLKARKIDHRILNAKYHEMEAQIVAQAGRLGAVTVATNMAGRGTDIVLGGNPLFLARE
                     EAARQGFDENSQPEKFSRVLEEKQKECEKEHARVVELGGLKIIGTERHESRRIDNQLR
                     GRSGRQGDPGCSTFYLSLEDDLLRLFGSDKISGFMEKLGLEEGEYIEHGLLTRAIESA
                     QKRVEEFHFDMRRQLLMYDNVMNQQREAIYTERRRILSEPDLTSHGRQLIEDALDSIL
                     ESFFPEEGEPQPQAVALQLKGVFWPGIERHISGVEEPEDLESARENIMGDLHERFEEK
                     FRDLGDENSTGMIRFLLLSVLDSAWKEHLLSMDELRRGIGLRAIGQKDPLLEYQFESY
                     NLFQSMMERVRISFADYFFKVTVMTGETSGKTPASRGRESRENLLSLPGSAVPNTQTG
                     KPPARSVKKTGRNEPCPCGSGKKYKHCCGRNI"
     gene            68551..70371
                     /locus_tag="P5541_00345"
     CDS             68551..70371
                     /locus_tag="P5541_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70453.1"
                     /translation="MRPRTILAMMLLVLFMGLCGVANARDIPAELARSLREAPSLESL
                     GSGQGLVLLRDISYRLLADGRMERTTLWFIHEEKGLPDSWRKWEVVVPEGGEASVAEA
                     GLYDPGSGKIQYPLIPRETSRDGASLIEVRVPNSLDGNIFVLSYRQVFPTRMNIEDSV
                     LVDLDIPQWEQRITLKVPSGTLPEWSGEGIPDPQVEKGSSTDSYSWSIVNTLARENGT
                     LSAEPGRVLVLSLRKGLRYSLAEAMSVADSIGVSPPSQVVSMLSDQNRTRGGERVIDF
                     VNDPSLIIRGLPEILARPVELIPAEGPWTEWEAAFLLANWLKKADWGADILWEARTTL
                     RDDSPATITSLRKPVLALTPPGGKQFMFEIGQGVKPGQVPPRLWGRTLYSLDGNTVLR
                     RQIPAGGPADHRLSFNWKLGLSPEGFAEGDLVINVRGAWVDTISAGNLPDEKQALDML
                     MAFGWPSTPGMPGGEAVVEPAGSGFRITLPVRAQMGIPSGEGLLMRMPSALMPWQMII
                     AEKGVSGGIRFPFVYEQSIEIELPEGFEVMVLPVLRPVEAGSVKTGESLRVKKGRTLV
                     GEQKVVISSARMDDQTRQVFANSIRQGLGWSGITVPMRKR"
     gene            70389..72068
                     /gene="argS"
                     /locus_tag="P5541_00350"
     CDS             70389..72068
                     /gene="argS"
                     /locus_tag="P5541_00350"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRV70454.1"
                     /translation="MKRDITEVLREMLERSIGKMACERGIEFREGPGIEIERTRREGQ
                     GDWATNAAMQWARIFREKPLSIAEKIVSGLEGDPLIEKIEIAGPGFINFYLSDSWMSG
                     ILGHILEHGDDFGRVDTGGGRKVQVEFVSANPTGPLHVGHGRGAAVGDICARILSFAG
                     WDVQREYYINDAGLQMDLLGRSTQARYFEIGGSPEKAPFPEDGYKGDYIYDLARDVMN
                     SEGDRFLLESPDDSFPFFKDFAGKVILDGIKKDLSDFGVEFDVWFSERSLYDGVRVPA
                     MLETLKKNGFAFDSEGATWFKATAFSDDKDRVLIRSNGVPTYFASDIAYHKDKYDRGF
                     DMVIDVWGADHHGYIPRMKAAVQALGRKESDLQILLIQFVNLLRGGRQVSMSTRSGEF
                     VTLREVIDEVGVSATRYFFAMRRSDSHLDFDLDLAKSSSNENPVFYVQYAHARIHSIL
                     REAESRETIVPSFDEANISLLEEPEEIALIKRLSAFPEEVEKAADDLSPHRIVYYAYD
                     LAGDFHTFYNAHRVLGVEPDLEKARLLLSEATRIVLSNALNLLGISAPEQM"
     gene            72071..72376
                     /locus_tag="P5541_00355"
     CDS             72071..72376
                     /locus_tag="P5541_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HRV70455.1"
                     /translation="MIRLRWVALIAAGLCFLAIVGTAYVLELRKISRLSGIIDERMDR
                     LVTMTRDVQILKEKIIFYRTPEGVARLAREQFNLTYPDEWIFRIEIVSEDALPQEGH"
     gene            72453..72737
                     /gene="rpsF"
                     /locus_tag="P5541_00360"
     CDS             72453..72737
                     /gene="rpsF"
                     /locus_tag="P5541_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRV70456.1"
                     /translation="MRPYEMVTIFQADLEDHKTAVEDIEGVVRNLGGEIEKSSSWGKR
                     RFAYPIQKQTEGFYAVLNFSLDPSQVRELERLLKLKTQVIRHLVIALEEA"
     gene            72750..73238
                     /gene="ssb"
                     /locus_tag="P5541_00365"
     CDS             72750..73238
                     /gene="ssb"
                     /locus_tag="P5541_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRV70457.1"
                     /translation="MARGFNKTILMGNLARDPEVRYTTSKQAVAKMTVAVNRQWKNKD
                     GEVQDQADFIPVVVWGSQAENCERFLRKGSPVLVEGRIQVRSYEAGSGERRWVTEVVA
                     QGVTFLGTRKDSDGYSQETAQGSGSRSGSLREKGFGDDFVMDISEVNNDGEEGEEADI
                     PF"
     gene            73274..73525
                     /gene="rpsR"
                     /locus_tag="P5541_00370"
     CDS             73274..73525
                     /gene="rpsR"
                     /locus_tag="P5541_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRV70458.1"
                     /translation="MAFTPRNNSSKRTKRRKPKVCQFCVDKVGHIDYKEVDKLKKFLS
                     ERGKIVPRRVTGNCAKHQRQLTEAIKRVRYLALLPYSAE"
     gene            73597..74556
                     /locus_tag="P5541_00375"
     CDS             73597..74556
                     /locus_tag="P5541_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HRV70459.1"
                     /translation="MDRTRELVESSLLVALAAALFLAGNFLPLVGMAFSLVCPAPLVI
                     LGLRHSLGRSILGVIVATVIVAVFTGVVGALFFCLGFGSLGIALGFFARRYDRAVDII
                     LYGILVSLGSKLILMFLVVKVTGINPFSLDEAELLSVMDKIGSVYSSMGLPEDAVSSV
                     SEQMRLALGLVPVIFPSLLVMAATVDCFLSYLVSGVVLKKLGKERVPEVPGFSEWRFP
                     KSIFLALLASIAMHFAGSWYPSLSLFQRAGLNLRLLVTVLFFIQGLAVVWSFMRARGL
                     GRFLRITFTMLIILVSFLAQIALILGIIDMWFDLRTRFRGGKA"
     gene            74553..75008
                     /gene="rplI"
                     /locus_tag="P5541_00380"
     CDS             74553..75008
                     /gene="rplI"
                     /locus_tag="P5541_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRV70460.1"
                     /translation="MKVILLSDVAKLGRKGDLIEVSEGYGRNYLVPRKMAEEATREKL
                     EEWKQRQNAMEKKARKEEEEARTHRNLIQGKQVVLRATAGDKGKLFGSVTAAQIADGI
                     KEKFGIDVDRKDIRLPGQIKEVGSYPLSIKLYSGVEAEMTVLVEAEKVE"
     gene            75001..76350
                     /gene="dnaB"
                     /locus_tag="P5541_00385"
     CDS             75001..76350
                     /gene="dnaB"
                     /locus_tag="P5541_00385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRV70461.1"
                     /translation="MSDRLFERIPPHSIEAERAVLGDCLLDQDALTITLDYLLPDDFY
                     DGSHRKAFSVMREMAEKGKPVDPLTFLDELSRRGLVESLGGQAFIAGFVDSVPTTANV
                     EYHVRIVRDKSVHRRLIQVGAEIARLGYSEDRELEEVLDEAERSVFEISQRGSQVPYR
                     RVGDVLGPAFHEIEEKFHSPDSSVTGIPSGFSDFERLTGGFQPGSLNIIAARPSMGKT
                     ALALDIAMNASVERDIPVLMFSLEMGSEQLVQRMLGSRAKVNIHDLRTGMFHENAWGD
                     LAQAAGDLSRAPFYIDDSSVLSTLEMRARCRRFKSQVLKTGGGLIVVDYLQLMQFSRR
                     IESKQQEVAEISRSLKAIARELDVPVIALSQLSRAVESRNEKRPQLSDLRDSGAIEQD
                     ADLVVLLYRSGYYESAQEEDDATAEVIIAKHRNGPTGTVRLTFFKEHARFANQAWSN"
     gene            76370..77158
                     /locus_tag="P5541_00390"
     CDS             76370..77158
                     /locus_tag="P5541_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRV70462.1"
                     /translation="MELNGLLKVSPEIIDRDPDQPRRIFDEAALKGLARSITEVGLLQ
                     PLLVRGKGNRFCLIAGERRLIASRMAGLDSVPVVVVDPDPALRSVLALVENIHRKDLS
                     SLEQAKAIGGIIEKTGWTQSETGRRIGLSQPSIANKMRLLDLDERVQEMVLDGTLGER
                     HARALIPLSPQIQREIAERSVREGLTARAIEKIAARARKGTADDDVQKKASLLQKEIM
                     SEISEAVRRRREKGLDVRMKIRTVGSHKRRLEILVTIENEEALQ"
     gene            77155..78984
                     /locus_tag="P5541_00395"
     CDS             77155..78984
                     /locus_tag="P5541_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRV70463.1"
                     /translation="MTRGKAEEPVTGDMFSLNNGTVVTGLLAVALVLPNLLFSGTWWY
                     ESLHIIKWAAVFIPVGLLVFAAGYRIASGRGRDPVLDPFGVVWLFLVLLLLMQPLWAP
                     VRSIPTFAREWFFFAALWGVYVVSRGGLKKGSLSLFLWGASFNGAINVLFAEIQIRGL
                     QGIFPFIYPVSGLYIGNTGQQNMFGLWLAIAMFGSASLFYNTADKGQGKNSLPLAVIL
                     NLLLFSICSWGLWNTTSRSAVLSFLVGLVVMTFLAVRNSNRRAILLRLAALAAIMAVT
                     FSGTAYLGRGNAFIMKTRDMIENFQTVGKRDSIWAASGTMFLMHPVRGVGLGHFKWNY
                     LEAQREMLLRHPGMKWQYTYWAHNEILQWFCETGIVGGLILLAMGVWWLSAFFSHVRK
                     GKPLSREAMWACSFLFLIWFNALWTRPFHRIEDAVWMALAFALANREILPSRAEWTEI
                     RRGWVLRGLGVAMAAGALSGLFFLGSGMAGDRILRVAASEKSMPEQRLLLERASRRLM
                     VSDIAERRLALHYIRLAEETKMGQYLDEGLTRLHLYFLRQPHVRDMIRLIAWYSDVGE
                     AGLMESVARYLRPGSYTITGGKINLSSEDERRFFGQSDNQYTR"
     gene            complement(79056..81167)
                     /locus_tag="P5541_00400"
     CDS             complement(79056..81167)
                     /locus_tag="P5541_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRV70464.1"
                     /translation="MRESPAKTFGSNVFNDRAMRERLPKHVYQKLQEAISGGEKMDFH
                     VAEIVATAIKEWAVSCGATHYTHWFHPRTDLTAEKHMAFLTIDEHGIPLDSFGGADLI
                     QSEPDASSLPSGGMRSTFEARGYTAWDPTSPAFVVPAEHGGTLCIPSVFISNDGTPLD
                     MKTPLLRALSRIENISLRVLKLFGNRSVKWTRATMGAEQEFFLIDESCAVKRPDIQYC
                     GRTLLGAPTPKGQQMEDHYFGSIPSRVLAFMQDVDKRMCSLGMVLKTRHNEVAPCQFE
                     FAPQFTEANLAVDQNQILMEQMRQIASSHGFRLMLHEKPFALINGSGKHLNFSLMDSE
                     GRNLLKTSSSQRKNIQFLTLVSALLLGISRYGGLLRASVATPGNMRRLGGNEAPPSIM
                     SVFLGDVLSGIFERVEEGIPENMPSKAFIDLGLNRLPDVLKDNTDRNRTAPIAFTGNK
                     FEFRAPGSSQSPAGPMTMLLSMWAWGMEELANMIESRIGETDVADAALDAIRYAAMES
                     RNIRFEGNCYSPEWHDEAQKRGLPIAHSTPEALALFLVPEHRELLSKLGIFTEREIEG
                     YYETRLEQYVKTLDIEMNVMESMVRNGILPAISSHITSEGTALAHVPESARAGSSSHW
                     QEHIEELYRMKERLLDGVAKMASLRNSLSSLDLEQRASVLTEKAIPLMDGIRGMCDAS
                     EVIVSTGFWPYPTYCEMLMGK"
     gene            complement(81317..81973)
                     /locus_tag="P5541_00405"
     CDS             complement(81317..81973)
                     /locus_tag="P5541_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV70465.1"
                     /translation="MIGITNGENATASEQVYLELKKRIFARELLPGQRLPEASLAKSL
                     SVSRTPVREALRMLASEGLVTITPNSGARLANPSLEEMTEGYEVREELESISVRRAAR
                     FITPLQICMLNEAIEEEEVIFSSRDLEKYLEVNIRFHRIIAGSSKNRILADYVGNILS
                     RTYVYMVFFESFFDFDTNPSLDEHREIVRSLAMHDEKLSERLMRDHIHLSMKALRKPF
                     "
     gene            82108..82848
                     /locus_tag="P5541_00410"
     CDS             82108..82848
                     /locus_tag="P5541_00410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV70466.1"
                     /translation="MYPDLSGKRIVITGGGSGIGLATARRFAREGSRVCVIDRDSSSL
                     DRAREELAGEGLFLCADVSDSSSVDTAFGRIDESMGGLDVLVSNAGISVRKTFREITY
                     EQWSKVLEVNLGGMFLCAREAAARMKDGGVILMTASTNGMEGHPFYADYNSSKAGVIL
                     LAKTLALEFAPRIRVNSVSPGYVLTPMQRAEYTPEMLEKVNEKIPFRRHAAPAEVAAL
                     FAFLASSEASYITGANIPIDGGETAGLQ"
     gene            82862..83629
                     /locus_tag="P5541_00415"
     CDS             82862..83629
                     /locus_tag="P5541_00415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV70467.1"
                     /translation="MEHEELFRLDGRTAVITGGASGIGLGCCKFLSMMGASVVLADLD
                     EGKGRTAEGEIGGRSLFVKCDVTSDEDCRNMASAAVARFGRVDYLVNCAGVIRRKNVV
                     DLTEKEWDLTLDVTLKGAFLASKYLIPFMARIGGGSIVNIASGWGVKGGPRAVSYCAA
                     KGGIINMTRAMAIDHGHQNIRVNSVSPGDTDTPLLRDEGRQLGVDEDKWLAESAERPL
                     RRLGRPDDIAMAVYFLVSGLSPWITGANLVVDGGGIA"
     gene            83655..85046
                     /gene="gcvPA"
                     /locus_tag="P5541_00420"
     CDS             83655..85046
                     /gene="gcvPA"
                     /locus_tag="P5541_00420"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRV70468.1"
                     /translation="MEEKKRVYPYIPNSEPGVRETMLREIGVETIDDLLPDVPDSIRM
                     KGRMNLPDPLCSEPDLRRHVEGLLSENVTCGECLSFLGSGCYDHHVPAVVDEIIGRSE
                     FLTAYAGEPYEDHGRFQALFEYQSMMSGLMDFDVCNVPTYDGSQAAGTSLRMASRITG
                     RKKVLLAGNMNPDRMRIVKTYIDEALSPELISWSPDTGLVDLQDLGRKLGPDVAAVYI
                     ENPSYLGGIEHRGAEIGAMTHDAGAIFIVSPDPSSLGILQPPSRYGADIACGDIQSLG
                     IHMNFGGGVGGFICTSSDRRFVEEYPSRLFGIAPTSKGEWGFGDVAWERTSFANRETA
                     KEFVGTHAALWGIAAAVYLASMGPQGMADLGKVITRRSLYARKVLGDLPGVSTARPSA
                     VCFKEFVVDFAGTGKTVAEINGKLLEKRIFGGKDLSSDFPELGPCALYCVTEKITVDD
                     IDRLASALAEIIA"
     gene            85080..86645
                     /gene="gcvPB"
                     /locus_tag="P5541_00425"
     CDS             85080..86645
                     /gene="gcvPB"
                     /locus_tag="P5541_00425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRV70469.1"
                     /translation="MTENLRPFHHMRWNEPIIYQLSEPGQRGILVPEAEEKITAMAGD
                     GMSLIPSAIRRSDLPDLPEMAQPQVVRHFNRLSQENLGADSNIDIGQGTCTMKHSPKI
                     NETFARSPKITDLHPYQDVSTVQGILEIFYRMEEIFREISGLDFCSVQPGGGAHGALA
                     LASIVRAWHRDRGANETRDEVITTLFSHPADAAVPKVKGYKVIVLPPDEEGLPDLEAF
                     RSVVSNRTAAIFFTNPEDTGIFNARIREFTDLAHQHGALCCYDQANANGLLGIARARE
                     AGFDMSFFNLHKTFSSPHGCGGPATGLIMVDEKLACHLPVPVVKKDENRGLFYLDWNL
                     PKSCGKIKDFFGVAPVVVRAYAWIMSLGAEGLEEASRTAVLNNNYFMKKMLDIRGCSL
                     SYPNHPSRVEQARYSWGKMKEETGFGTNDVQRRIADFGTHYWGSHEPWIVPEPFTIEP
                     SESYSKSDMDEYLEIMARISRECYEEPEVIAHAPHNSTIHHIDHDYLDDPDKWALTWR
                     EYNKKYEGYFRRK"
     gene            86654..87784
                     /locus_tag="P5541_00430"
     CDS             86654..87784
                     /locus_tag="P5541_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HRV70470.1"
                     /translation="MAENIRRLGLIVNPVAGMGGSVGLKGTDGLDILERAIALGAKPG
                     ANRRAMEALERLCDYRKQLEIVTFPGEMGATVSNRAGFRHSVLRGTSRLERTSSEDTV
                     KAAGLLMSGGVDIILFAGGDGTARDIVSAVGTGIPVIGIPAGVKIHSPVFARNPSAAG
                     ELARLFLFGPPTGLRELEVMDIDEESFRQGRVSTSLFGYLMVPYEKSHLQGGKSGSPR
                     SEAVEVDGAAREAVSRMLPGVLYLVGPGSTTASLMGIMGCPFSLLGVDAVRDGKILGK
                     DLSESEILEMIEPGNTRIIITVIGGQGFIFGRGNQQIGPGVIRMVGKKNITVVAAPSK
                     LRSLPGRSIFVDTGDTQLDEDLCGYMRVITGPSEETLVSVRR"
     gene            complement(87835..88383)
                     /locus_tag="P5541_00435"
     CDS             complement(87835..88383)
                     /locus_tag="P5541_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV70471.1"
                     /translation="MKRILALLGSPRPGGNSETLTQAVLRGAGSKGNEISEIRLNGMT
                     LGGCIDCRKCWSRGNPCIIVDDMNGIHDRIRQADVIVFSTPLYWYTWSSQIKPVWDRF
                     LPFLHENAAWDLRGKEALLVATAGDDRTDAFEGLLFSFRTSCDLLGIQRTEPLLACGV
                     HEKGDILKGDWVKKAEERGRAL"
     gene            complement(88380..89282)
                     /locus_tag="P5541_00440"
     CDS             complement(88380..89282)
                     /locus_tag="P5541_00440"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRV70472.1"
                     /translation="MKLEGIYAPVPTPFDRSGNVDHKNLEFNFRKWMNLPLDGIVVSG
                     SNGECPLLSFRERVDLFGASAALAKGKIKVIAGVHCPSTRETAELAIEASRAGVDALL
                     LLPPHYYKGQNSTESLEKFFFEIADSSPAPLVLYNMPSNTGVNLGASLVAKIATHGNI
                     IGIKDSSADIVQIATICRDAPEGFGVFAGSGSYFLPSLAVGCSGGTMGVANLFAGPCR
                     DIFASFKKGNLDRARRIQLSMIGINQAVTARFGVPGLKSAMDHAGLYGGPVRPPLLDA
                     DETTKREIAGIFDKFRQFYEEEYA"
     gene            89394..90344
                     /locus_tag="P5541_00445"
     CDS             89394..90344
                     /locus_tag="P5541_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRV70473.1"
                     /translation="MKEFRIGTCSWTDRSLLSSGWYPDSARDAQSRLRHYSGFFDTVE
                     VDSTFYAIPDESSFYHWASRTSPGFLFNVKAFGLFTFHTLPVSSLPAQIRPKNASQGS
                     KIRIGDITRDQRVGLWNAFVSRVLVLHRMERLGYILFQFPPWVTFSEKNLRWFRRIGD
                     LAAPMRVALEVRHRSWVENENRERLLEVLREENMALAAVDEPKLDWTVPFDWSITATW
                     GTVLRFHGRNAPAWSKRNASVAERFNYLYDTGELEAFREASERLPDDTGRVFLMFNNC
                     FRDNAVRNALQMRKIMGFGAIVPPGQESLGLGEVAPDENE"
     gene            90378..91355
                     /locus_tag="P5541_00450"
     CDS             90378..91355
                     /locus_tag="P5541_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRV70474.1"
                     /translation="MIWMRIVFSPVTFQKLESVIGRMFAGHEVIISDLADLPEKIAGA
                     EVLVTGPLVVRDDLLERSPSLRLVHQWGVGVEKIDLDACSARGIRVCNVPSMGTGNAE
                     GVAEIALMHMLLLSRRFDRSRESLANRRLFSPQGVSLWKKRACVVGLGDVGKSIAGRL
                     SCMGMKITGVNRSHREDLERWGIDRLYKPGEVAYALPGCRFVILAMELSDETREIVDG
                     GFLRAMDNDAYLINVARAELVAREALETALRKGDIAGAGLDVFWNEPADPDDPLLNNP
                     LVTITPHIGGVTDEAIPGVASYVAENVTRLQRGVSLLNCLNCSAGEKEG"
     gene            91361..92032
                     /locus_tag="P5541_00455"
     CDS             91361..92032
                     /locus_tag="P5541_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRV70475.1"
                     /translation="MKKAVLFDFDMTLIDSSHGVTFCLNMVAERFGLEKVERPEVLKT
                     IGYPMEEAMEMLWGAFDPSWTNHYRDYLVPFEYERLAPFAGVRETLEKLRANDVVMAV
                     VSNRRRLLPALAGAGLEDFFSMAVGMEDVSAPKPDPEAINLVLDRLDVSRERAFFVGD
                     SEIDAIAARNAGVRFIGITTGGRSASVLLEEGAFAVLEDMSKILRVVCPGFCDVPLAQ
                     DRGVL"
     gene            92040..92963
                     /locus_tag="P5541_00460"
     CDS             92040..92963
                     /locus_tag="P5541_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70476.1"
                     /translation="MKQTKLVRTFYYFFAACIILGALLVYKAWWDRYFRLRPELATAE
                     PAVLSIDVPVDVALLWEESIIVSPSTGKISFPVGRGPLYCPKGETIAVISGSSGKKQI
                     RMPGPGYFMAALDGQEGKWSYGDLWPGSNRIPSIQKPVFIAEGVAVLQGEPIGKFVPQ
                     PRQLRCVAYIDSATVAENFSAMEYLEIKRDPADLPFRAEIRAARDLGPALKVYLTLPF
                     FPGEILLSRTMSFLHHTGQSSGVMIPESSVTLRGGQKVVFLVTGDRSRAVKVEGIPVS
                     GKRFLVESGLSPGDLVVVSGSEAEEGEIRIW"
     gene            92957..93655
                     /locus_tag="P5541_00465"
     CDS             92957..93655
                     /locus_tag="P5541_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRV70477.1"
                     /translation="MVSAISCNIQNIRERILKAALAAGRNGDDVSLVAVTKTRSVREM
                     IEAASCGVEALAENRVQEALSKHEEWPPELDISWHLVGHLQKNKARKAAAIFDYIHSV
                     DSIQLANTLEAILQPEGNIVRILVEVNVGFDPNKNGVLPGDLPFLVEEILSDCHSLSL
                     CGFMTVLPLGIDEKMTRTLFARLRQLREEMVSRFGIPFPELSMGMSGDFEYAVMEGST
                     MVRIGSAIFGERQR"
     gene            93691..94317
                     /locus_tag="P5541_00470"
     CDS             93691..94317
                     /locus_tag="P5541_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRV70478.1"
                     /translation="MSDLLTVIDIENINFRKSMRGYDPDEVEEFLEKVAGSLQIYSEK
                     IKNLEVQVSRFDDQMKEYVELKRSLQDALVLAQKSAEERVNSAESRAEVIIAEAEAQA
                     HKIVIGAKDRVDEHKREIERLRQIRYQFVAEFKGLLAKFGSMLNVLEAPPEDREQERA
                     PERSRERERYGEHSSKSDCEEGQETVAEPGGSGDVSGLETARYGEERS"
     gene            94314..96395
                     /gene="recG"
                     /locus_tag="P5541_00475"
     CDS             94314..96395
                     /gene="recG"
                     /locus_tag="P5541_00475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRV70479.1"
                     /translation="MTESLAWDSHVRFLKGVGPARASRLGRLGVDTVEDLLYFFPRRY
                     EDRRSIIDLSRLEVGSVSHAIASVVSIERRPTRKKNLSIVNALLSDGSSMAQAVWFNR
                     KGLERVLIPGTRASFYGRVESRGGVVQIVNPEFEILEDKDADTPGLFAGIVPVYPGTE
                     GLFHRWVRSLVFNAVETFAANLPDPLPPEIREKNRLVSIEEAVRSMHFPEGRESWSAA
                     RKRLAFDELFLLQTALALRKRSYEEETGGYPLSWDGPILQKFRKEILPFELTSEQEKV
                     LGEITRDSRRNAPMNRLLQGDVGSGKTVVALMFLLAAVDSGFQGAIMAPTEVLARQHH
                     RKISNWLSGTGVEVLYLSGSMTSSQKTEALAHVMSGRPGIAVGTHALIQKGVRFGRLG
                     AVVVDEQHRFGVLQRSALAEKGQCPHVLVMTATPIPRTLTLSVYGDLSFSTIEELPPG
                     RTPPVTKQISNAGDPRLIRFMEEEISGGGAIYWVCPVIEENGSLPLTPVIGRHRILSE
                     IFGQERVGILHGQVPSGERERIMDLFSTGKLSVLVSTTVVEVGLDVPEATVMIIEDAH
                     RFGLSQLHQLRGRVGRSSRRSYCFLVGNPKTPEGIARLKAMCSTADGFRIAEADLSLR
                     GPGELCGIRQHGVTDFKVADLSRDRVLLDVCREDAINLVSKDPLLDGYPVLRKEVFRK
                     LGRKLKLVETA"
     gene            96429..96842
                     /locus_tag="P5541_00480"
     CDS             96429..96842
                     /locus_tag="P5541_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRV70480.1"
                     /translation="MSYAVRLTSRSEYTCAQVRRKLLARGAPELQAKEICSELESCGL
                     VNDVRYCELFVSTHPEFGFSRLKMELSKRGVERAVIESTILMDEDDEISRAVLLAREW
                     ADFTEPRKIAARLSRRGFSARVITSAIERACDTSS"
     gene            96941..98470
                     /gene="rny"
                     /locus_tag="P5541_00485"
     CDS             96941..98470
                     /gene="rny"
                     /locus_tag="P5541_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRV70481.1"
                     /translation="MLPVMLAAAFAVGIPIGFLAYKFMSDKKLKGTREQSDQLLKDAF
                     QEADRRKREMLTEAREEIHRLRQDAEREIKDRRAELQRAERRLEQKEENLDAKMDAIS
                     QREEDFRKRQEDLSARLEEIQSIRQKQVERLEEIARMSREQAKDVLLQEVEQEAAHTI
                     GLRLKELEEKAKRESERKSREIVVSAIQRCAVDHTSEMSVSVVSLPSDEMKGRIIGRE
                     GRNIRAFETFTGVDLIVDDTPEAVTLSSFDPVRREVARLSLERLVLDGRIHPARIEEI
                     VEKAQKDVEEKILESGEEALLETGIKSMHPELVKIIGQLRYRSSYGQNALSHSLEVSH
                     LAGIMAAELGLDEQMARRAGLLHDIGKAVDHQVEGPHALIGRDIAKRFGEIPEVVNSI
                     AAHHEDVEMTSVYDVLVSSADAISASRPGARRESLESYIKRLEKLESLAKTFKGVTKA
                     YAIQAGREVRVMVAPNSLDDGAIHKLAYDMARKIEGEMKYPGQIKVIVIRETRSVEFA
                     K"
     gene            98482..99258
                     /locus_tag="P5541_00490"
     CDS             98482..99258
                     /locus_tag="P5541_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRV70482.1"
                     /translation="MRILFVGDIVGKPGRKVFSSVLPGARQSDGPFDFVVVNAENAAG
                     GFGLTAKIMQELFDIGADILTSGNHIWDNRDALPLLDEESRLLRPANYPPEARGRGMA
                     TFEKKGLKLAVLNLQGRIFMQALDCPFRRADEELTSLEAGIPVLVDVHAEATSEKRAL
                     ALYLDGRVSAVVGTHTHVQTADEEILEGGTAFISDVGMTGGHGGIIGMESESIISRFL
                     TGMPSKFVVCGKDSRMDAVVIDIDEKTGKSISIKRLSLDS"
     gene            complement(99368..100354)
                     /gene="hypE"
                     /locus_tag="P5541_00495"
     CDS             complement(99368..100354)
                     /gene="hypE"
                     /locus_tag="P5541_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRV70483.1"
                     /translation="METISIGHGAGGRLTEDLVSTILGNFPGRPVADREDCALLPGGL
                     AMTIDGYTVTPPDFAGGNIGSLCICGSANDLAVRGAKPLYIALAVIAEEGFPLSSLSR
                     YMESAGEICEMIGIELVTGDTKVVPKGQADGLFLTTCAVGKTRSSSKLGTANISPGDS
                     IIITSPPGMHGATIAAVRYGLNVHGLFSDCAALWPMIEEMLPIEGIRCMRDCTRGGLG
                     TVMCEWAESAGVGIEIDETLIPWDEASLSVCDLLGLDPLHMAGEGCAALVVSAEKTEE
                     VLAAARNNPLGKRASLIGSVTEAHPGYVGIHTLSGGTRLVDKPVGEELPRIC"
     gene            complement(100336..101448)
                     /gene="hypD"
                     /locus_tag="P5541_00500"
     CDS             complement(100336..101448)
                     /gene="hypD"
                     /locus_tag="P5541_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRV70484.1"
                     /translation="MPTNEMSETRDKVSLLTKALDILADRPLRVMEVCGTHTVSIFRQ
                     GLRSLFPPNLELVSGPGCPVCVTDQGEIDAAISLASLDGVITATYGDMLRVPGSGGSL
                     LDARSRGKDVRIITSAAQALDIARMTPEKLVVLVAVGFETTAPATASVVLDAQKNDIR
                     NFAVLNLHKAVPPVLEALASDSRIRLEALLLPGHVSVVIGLHPYQFLPAKYGIACSVA
                     GFGAEDIMLGLVEIARQVREDRPRVAQMYGQAARNYGNPVAQKLLEKVFCLRDTSWRG
                     LGEIPLSGYAFREDFSSLDALNRLGLKISRTPPPQGCSCGEVLMGLIGPNECPLFGRT
                     CTPSHPVGPCMVSGEGSCSAHYRFFTGGDDPWKRSA"
     gene            complement(101435..101662)
                     /locus_tag="P5541_00505"
     CDS             complement(101435..101662)
                     /locus_tag="P5541_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRV70485.1"
                     /translation="MCLAIPHRIRSLIDETRALASAGAVETEIRTDFVPGVEVGDVVL
                     VHAGFAIEHLLEENGREVEALWEEVRRLANE"
     gene            101798..102592
                     /locus_tag="P5541_00510"
     CDS             101798..102592
                     /locus_tag="P5541_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV70486.1"
                     /translation="MDRIRVGVLQLSVVREDAAKNSRYVGGEIRDAARGGCDVIVLPE
                     LWNAAFPLLGPHVLVDEKSGIVEEIAGAAFRTGTWIIAGSMAMRTPLGPRNRCLVFSP
                     EGNIFFRYDKIHLYPGLREPNILSPGSKLGFFDLDGIPCGVMICFDAEFPEVASALSR
                     KGARILFVPGAWKSEYIRLWRAILLSRAIENQVFVVGVNRCDRGKESSFGGHSMVIDP
                     YGDVLLHLDDSPRFQMVNLDLGKTISARREHKVIDSRRPGVYRRWN"
     gene            102878..104218
                     /locus_tag="P5541_00515"
     CDS             102878..104218
                     /locus_tag="P5541_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRV70487.1"
                     /translation="MSNGTEREQWGSKLGFVLAAAGSAVGLGNIWRFPYITGQNGGAA
                     FVLLYLALVFVIGFSVMLAEIAIGRNAQLNAVGSFRKIKGGIWPLVGWMGVIAGFIIL
                     SYYGVIAGWTMAYILKSFTGLMGAAAEGAAGDVFGGFITNTAQAVAWQALFMVITIYI
                     VYKGIGGGIEKYCKVLMPALFLLLLVLIVRSVTLPGAGEGVSFYLKPDFSKLTAGAMG
                     SALGQAFFSLSLGMGCMITYGSYLGKKEMLPGAAIQVCFLDSLVAFLAGLVIFPAVFA
                     FGLDAGAGPGLTFITLPNVFAQMPGGAVWSAMFFLLLFVAALTSAISLLEVVAAYFID
                     EMKWTRSKAAWVMGGVIFLLGIPSTLGISGVIPKLAGRDFLDSADFLASNVLLPLGGF
                     FIALFVGWIWTDGAKQEVENGGAAPFGLYVPWLWVCRVVAPLAIAYIFITGLKW"
     regulatory      104318..104427
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(104620..104946)
                     /locus_tag="P5541_00520"
     CDS             complement(104620..104946)
                     /locus_tag="P5541_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70488.1"
                     /translation="MITLSGSGLPYTIFFEAFTQKAGIKLGIIFKDCGGMTGRWPPAF
                     FGRRKKQYRFFEHAKHLGIRSGPFFRIRPTDQKGPVRLLKGLYVSVKGVGKKMPTGMD
                     PVSGQF"
     gene            105008..105325
                     /locus_tag="P5541_00525"
     CDS             105008..105325
                     /locus_tag="P5541_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRV70489.1"
                     /translation="MIEVTKDNFEEEVLKSDMPVLVDLWGPKCGPCLALMPQVEELSH
                     EFGDKVKFCKLNVAENRKLVISLRVMGVPTFLFYKGGEIVDRLSGAEAVPEAIRARVE
                     KMN"
     gene            105360..106646
                     /locus_tag="P5541_00530"
     CDS             105360..106646
                     /locus_tag="P5541_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HRV70490.1"
                     /translation="MELKIQKVLVKALSEGDKTSFNAGTLTINRQEIIDMLLKDQRFT
                     SVKVELARPGESIRIIPVKDVVEPRCKVEGPGEVFPGFIGDVDTVGEGTTLVLSGAAV
                     VTCGKIVGFQEGIIDMSGPGAEYTPFSHTFNIVLVVEPTEGLEKHDYESACRLAGLKT
                     AHYLASKVKGSASGEVETFAIPDLAKAMKSHEGLPKVAYLYMLQSQGLLHDTYVYGVD
                     AKKIIPTLIHPNEVMDGAIVSGNCVSACDKNSTYSHQNNPIIDHMYKRHGKDFNFVGC
                     IITNENTTLADKKRSSSYAVKLAKMLGVDGVIISEEGFGNPDTDLIMNCRKAEQAGIK
                     TVLVTDEYAGRDGASQSLADACPEADAVVTAANANETIVLPRMDKVIGLPDTSNIIAG
                     GFDGSLRADGSIEVEIQAITGATSELGFSRIGAYTI"
     gene            106673..107149
                     /gene="grdA"
                     /locus_tag="P5541_00535"
     CDS             106673..107149
                     /gene="grdA"
                     /locus_tag="P5541_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:106802..106804,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HRV70491.1"
                     /translation="MGKLAGKKVLLLGERDGVAAPAMEESLKNCGADFVFSVTECFVU
                     TAAGAMDLQNQQRVKDAADKYGAENLVVILGSSDAEGAEIYAETVTNGDPTFAGPLAG
                     APLGLAVYHVFEKDIRDEAEPSLWEEQISMMEMVLDTEALANAVKGVREQYGKYTL"
     gene            107182..108489
                     /gene="grdB"
                     /locus_tag="P5541_00540"
     CDS             107182..108489
                     /gene="grdB"
                     /locus_tag="P5541_00540"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:108226..108228,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HRV70492.1"
                     /translation="MAYKVVHYINQFFAGIGGEDMAGHKPEARKGFVGPGQALAAAFG
                     SEAEVVGTVICGDTYFADNLEKAEKEVMDLIKTFSPDAVVTGPAFNAGRYGTACGSVC
                     EAVRKNLGIPTVSGMYHENPGVDMFRKSTYIVSTPDSARGMKEAIPAMAAIALKLLKG
                     ETLGKPEDEGYLERGVRVNFFMDKTGAERAVDMFVNKLAGKPFVTEYPMPTFDRVAPN
                     PPITDMKKAKVALVTSGGIVPKGNPDHIEASSASKYGEYSLAGVMKIDATTHQTAHGG
                     YDPTYANDNPNRVLPLDVMRDLEKEGVFAKFHEKFYTTVGNGTSVGNARRFGAEIAQK
                     LKDDNVDAVIVTSTUGTCTRCGATIVKEIERAGFPVAHVCTIVPISLTVGANRIIPAV
                     AIPHPLGDPSKSPEEEKKIRRAIVEKALRALQTPVEDQTVFND"
     gene            108559..110088
                     /gene="grdC"
                     /locus_tag="P5541_00545"
     CDS             108559..110088
                     /gene="grdC"
                     /locus_tag="P5541_00545"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HRV70493.1"
                     /translation="MAHVAIRGYSYCLNHTPEIGLYYGNTPYMEKVSHGETEFIKKLA
                     GSIQSFEEACCYPPNQAFIGGMDLDELSARLAPMFENRLEKGSRFGRFGEIMPENEFI
                     GLMDICDVFDLIWLEESFASDVAKALSVHPLFSEKLMARLEKGHSAAEVEEEISKHGG
                     LPLWWKGKAVGCCRKGHDTDESLSAHVLLENIASKAGSVLALLHLLNNTGKVPGDIDY
                     VIECSEEAAGDMNQRGGGNFAKAVAEIAGCVNASGFDVRSFCAGPVNAVIAGATQVAS
                     GARPNVVVLAGGAVPKLFMNSRDHVKKGMPALEDCLGNFAVLLEPDDGTCPVMRLDAL
                     GKHSVGAGASPQAITSALVLEPLQKIGLSFADVDKFAAELQIPEITVPAGAGDVPAAN
                     FKMIAALGVMKGQLKKEEMASFVEQKGIPGFAHTQGHIPSGVPFIGHACEAIKEGKLS
                     RVMIIGKGSLFLARLTNLSDGASFLIEPGKGEKPGAMTREDLREMLLDALQDLSKGLT
                     G"
     gene            110096..111253
                     /gene="grdD"
                     /locus_tag="P5541_00550"
     CDS             110096..111253
                     /gene="grdD"
                     /locus_tag="P5541_00550"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HRV70494.1"
                     /translation="MTTGGETRKIVGEVLKEIVESARSGGPLVRVGLMGSGSELGPEE
                     LARGALIAQGSRPGLRVVMIGPRLEGWDMLDWIETADCEEDISRAMETALQKGAISAA
                     VALHYPFPLGVTTIGRVMTPARGKPMFVASTTGTSSTERIEGMVKNAIYGVAVAKASG
                     IAEPSVGILNIDGAQLAFRALGRLRDNGFPVTFGSSVRKDGGAILRGNDILAGAVDVC
                     VTDTLTGNVLMKMFSAFTTGGGYESTGWGYGPSVGKGWPVIVSIISRASGAPVIANAL
                     QYSAMVAEGSLPKLVEEHIALAEKAGLEEILSELRPTRQAEEKVPPPPKEPTEEELHG
                     IDVLSIEDAVVALWKNGIYAESAMGCTGPVVKVPGRSLEEAGEILRKEGFL"
     gene            111439..112815
                     /locus_tag="P5541_00555"
     CDS             111439..112815
                     /locus_tag="P5541_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRV70495.1"
                     /translation="MEAFMRLNGVVNGIVWGPPMLALLVGTGIYLSVILGFPQVRYFG
                     FMFKEVLGKIGKKAEGEGTISSFGALAVALASTIGSGNIAGAATALHLGGPGALFWMW
                     VTAIFGMTTKMTEVSLAVKFREKDAAGNWRGGTMYVMEKALGQKWLAWLFAFFTTFAA
                     FGIGNAIQANSTAQALELGFGVPSYVSGIVIAVLVALVIIGGLKRISDVTTYLVPFMA
                     VFYVIGGIAVIVVHADLIPQAISNAVYYAFNDPMAMPGAIAGWSIKAALTRGVARGVF
                     SNEAGLGSAPMVHATAVTDHPGRQGVYGLFEVFMDTIVICSITCIGILTTGTLTARPD
                     LTGAQLTLTAFQTVLGTPGVMILSAGLAMFAFSTILGWYWYGETGAVYIFGLKIAPIY
                     KVLWIAVCVVGAWGGGGQFLANIWDLSDTMNGLMAAPNLIALLWLSNEMRKIVKDFDE
                     KRKKGTIK"
     gene            112909..114024
                     /gene="alr"
                     /locus_tag="P5541_00560"
     CDS             112909..114024
                     /gene="alr"
                     /locus_tag="P5541_00560"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRV70496.1"
                     /translation="MALRPTRMEVNLANLAFNCRSISSFLDPGCNMIAVVKADGYGVG
                     AMAAAKTFRECGCQRFAVATPDEAVELRDGGITEPILVLGPSPRESAEELVAGDITAA
                     CTDVAFAEAMSRAASARGKTARLHLKIDTGMGRIGFLPSEAPSAVEKITVLPGLELEG
                     IFTHFACADERNAEYTSIQFSRFGETLDLLRPLNARFRIRHVCNSAGTVNFPEMHLDA
                     VRPGLILYGMWPSPFCKRSVELKPVFEVKTSVAVVRELSPCSGVGYGLKYMTRGTERV
                     AILPIGYADGFARPLSMKASVLVRGMRAPLIGSICMDQTMVDVTHIPGVVTGDEVVIV
                     GDQGKERITPEETAAHLGTINYEIPSLFTKRVPRVYV"
     gene            114145..116091
                     /locus_tag="P5541_00565"
     CDS             114145..116091
                     /locus_tag="P5541_00565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV70497.1"
                     /translation="MNDLKSVLRGFPSDRLNDRSVSMIESAARECRSWAVTMTTAAGS
                     GHPAGSLSSMEIYLMVYGAANITPENCDNPDRDHVVISHGHTSPGAYAALALYGFFDP
                     GEAMAHFRQAGSPFQGHVEREVPGIDWGSGNLGQGLAAGVGFALAARARGRVDNVYVL
                     MGDGEQVKGQVAEARRIASHHGLDRITVLIDLNEMQISGRTEDIMKADIRGLWEIDGW
                     HVIECDGHDPAALYASMLEAERTRLPSVIICRTLMGKGVSFMEGIPDYHGKAAAGDLY
                     RRAMEELEAPADMLDKALAKRTTGPVLKGRNIVTCPPLLDTGTPANCDPSKMTDNRSA
                     FGNALADVGIRNYGVEGRTPILVFDCDLAGSVKVDGFAGKCPDWFVETGIQEHATATV
                     AGAASTAGVVGLWADFGVFGLAEVYNQQRLNDINGTSLKLVLTHVGLDVGEDGKTHQS
                     IDYMGLLRNTFGWRLLVPADPNQADRMTRWALGEPGNICLAMGRSKIPIILDENGKPF
                     FGGDYVFRYGAVDVIRRGSDAAILAVGHMVSRALRAWEILKSRGVNVSVYHVGCPLHL
                     DRDALSEAAGTGKVVTFEDHHVGSGLGSLVASKFVETGCCCELRTLGVTEYGESGTSA
                     DVFTSMGLNHEDLVDTVLGMKGIK"
     gene            116105..116734
                     /locus_tag="P5541_00570"
     CDS             116105..116734
                     /locus_tag="P5541_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRV70498.1"
                     /translation="MELMNLGGPVMWVILFLSVAGGAMAIERLWFTMKANTDASAVEL
                     ALGGHIYHGRKEEASKLVREKESSLHRLLRVGVDHWETDPGALQVLLEQEVRREVFRW
                     ERGLGFLATLARVLPLLGLLGTVLGMIDVFQNLPGMSGSSMTVMAGGIWKALLTTVAG
                     LGAAVPLILCHAFLSNRVERAEEMLWRGVDFMVREKILRNGTNEKTGME"
     gene            116712..117593
                     /locus_tag="P5541_00575"
     CDS             116712..117593
                     /locus_tag="P5541_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRV70499.1"
                     /translation="MKKPVWNNNILVLGLFLCFLAVAPVRSSAAFSGRIVSLAPSVTE
                     CLFSLGSGDLVAGVTDHDVFPEEVKELPSVGGYFDPSLEKILSLSPDLVIGISTFHGR
                     LLERLESMGVKTLPLTVHRGLEGVGDSLHSIGEKIGKRAMAEDLWRDIMEKIDLVAED
                     VKRSFPGGPPSLLVVVWHDPLTVAGGVNFIDDILTRIGIPNAAGDINYTFPQLDPEGV
                     IIRDPDIILVPRTERGMSLSPDVLENALKGLPIRAVEEGRIVSVSADLLFHPGPRVAE
                     AAMLLLGSIMQFEGLVQ"
     gene            117590..117979
                     /locus_tag="P5541_00580"
     CDS             117590..117979
                     /locus_tag="P5541_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRV70500.1"
                     /translation="MNRMRRPRAHPDVELTPLIDVLFMLIIFFVLTAVFTESSIPVNL
                     PEAQGDPSRERTFTLTIDAEGGVYAEGKILSIDLAVKRAFEAWQEGRKIVIAADRGVE
                     YGVVVSLLDQLRGQGVDSASLLVEATE"
     gene            117982..118590
                     /locus_tag="P5541_00585"
     CDS             117982..118590
                     /locus_tag="P5541_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRV70501.1"
                     /translation="MARWIPFFVLSVMIHLVVILIPFNYGSTELVDPRVISVEIETFS
                     KPSGETGSPGSETAPLPKKVPVKTGAIEKQLQPEAAFSRSDPVVAGEPLDDEEPEPKI
                     VAPGDGVKPSGGSATPVVTEADTIRRVTPVYPLASRRRGEEGEVRLLVGVARDGSTAS
                     VEVVGTSGFSALDEAAVFAVRRWRFAPGSPEKLIVPVIFRLE"
BASE COUNT        27468 a        28959 c        34604 g        27684 t
ORIGIN      
        1 caacctgaca aggacgccgc ttgatggctc atacaccaga tttgacaata actcctgatc
       61 cggcagtttt tccgttgaaa actcccggtt atcggtagcg atgtacatgc gacctatcag
      121 cccgggaatc tccttgagtt tccactcatg gtagattccc ttgcccccgg ttgtgtggtg
      181 tggttctccg aaatgactaa cgtaaaaatc cagcactttg tcaatatcat ccttggaata
      241 gaattgcctg gccattgggg tatcaggaac ataggggttt ccgatcggcc cctggccatg
      301 ctcaatattg acagaccctg gatagacagg agccaaaaga cttgaatgtg ccgggggtgc
      361 tgaaaagagg gtaaaaacaa taaacgtagc aataatggtg gaagaactat tccttttcaa
      421 aaaaatcacc tctttcgggg tggttttttt agcttgatgg atctgttcaa aacctccaat
      481 ctgaaggatg ggcccctcaa tttacaaagc acaattagta tttgtttgac attgtatcat
      541 gatgccgtaa aaacaggtag ttttctttcc tgccgtttcc tttactgccg ggaatcgcgg
      601 gagattgagc ggatccctga actgttttga tcttccagaa cataaaagaa caggatctat
      661 ccgtccgttt tatggtttca aatccacgct tttgacaagt ttaataaaga taggcaagaa
      721 tgggaaaaca taataggggt caactatttg gacaatcttc agatcggatg atctcttcaa
      781 aaatatccaa tatcgtttcg atagccctcg acatcgacca cctcgggaaa ttggttctcc
      841 tacgaaacat tggaagatca aaaggagtat ttttcgtggc gaaaagacaa tttagcatat
      901 actggcaaga atatatcaag gaggtcaaat gaaaaactga atattcaggt ttccgacagg
      961 tctcgaacct gacccttgtc agacttcact gaacgataat gccagtcatt cgggaggttg
     1021 tcatgaacag gagatcagtc ttatttgtgc tgtgcctttt gatgttgtcc attatccttg
     1081 tctcctcccc atcggaggct tccggttcgg ggtactggaa gctgatcgac gttgatgtcg
     1141 aacatcatga aacaaggatc gaaaactccg acggcagcaa gcttttgggg accatttcgg
     1201 ggggccccgg tcagttcgaa gcaacctttc tggaccagaa gagaaactgg aaaatggagt
     1261 attccgtctc gggcagctgg ggggagatcc ccgagatcct gatacttcgg gaggaggtat
     1321 ctgtacccct tgagctttcg gtcggcagtt attccatatc ccatccttca gacgtcggcc
     1381 ctttcgccga attggttgtt tatttttcct atagtagtga ttctgttgag gaagcgcgcc
     1441 cccgtaattt tgcggggcaa atcgaagtat ccgacaaggg gccttcgcac gtgactgcca
     1501 ctgccaaact tctccctccc gggcctcgaa gcggcaacag ccagtacttt gttgtcgaag
     1561 taaccgcaag gaacctgtca tcaatgcatt actggagtaa aacattcaaa tatgtctacg
     1621 cctggcaggc gggagagccc ccggcagaag cggacagggc agtggaatat gccgtcaaag
     1681 cggcaagtga tcctcgaact gtaagcccag acggtgaatc gaccgtcgag gtaagtgccc
     1741 tgctttatga atacgaccca ggtgacagcc tttcttcccg tcctgtcgct ggcaagacca
     1801 tttatttcac tgtcaatgaa caggacgggg ttatccccgg gactctatcg tcggcttccg
     1861 cggttaccga cgaatatgga aatgcaacgg tgaccttcac ggcgcctgaa ggcagccttt
     1921 taaaagatgc aaggcttgtg gtcacatccg cgacggttac ggccagagcg gaagaattag
     1981 acgttgagga tatggcgtat attaacttca ccccagagcg cggcaaggtg ttcgtgcaac
     2041 cgggtatctc gggtatcgtc tcgtctcacg ggatggttcc ccccgacagg agatttcccg
     2101 cccttatcgc ggccaacttt gaagacgagg atcttcagcc attgaaagat actgaggtga
     2161 ccttttctat ccgggaaggg gacaaccacg ggatgctgcg gtctcctgac ggcatcgagg
     2221 ctcgaaaggt cgttaccagg accgacccgg aaggatgggc tgaagttcaa tatttctacg
     2281 cttccgatga acctccggtg gatccggtga ctgagacaat agagataaga tcccggaaaa
     2341 tgatccttcc ccttacagcg gaagtgacga cgggattcaa cctggttttt gaacgcatgg
     2401 aaagcgccta tgaaggcaaa ggcaccgtca atgccggtga agagatacct ttcagggtca
     2461 atatcaagga tgcctggaat cccggtgtgg acctggaaaa ggttgtcggc ttctggggag
     2521 ttgatgaaag atcgggttcg gaaatgcttt ttgtgagact ggaagtcgag aacatcggta
     2581 cagttcccga ttttcttctg gatcagctaa aacttgaaaa gtatcccgag gcgctttttt
     2641 cagaggacat gcaggtcagg tctttcaggg acaagggcga gatgaacatg ctgtggatgt
     2701 ccgggaactc cctgaaaggc taccagggct acccaaggat cagacccatg accaccggca
     2761 atcattatta cgaggcaagg ctgtccctgg tcgatggaag tggccgggaa gttttccgga
     2821 cgaaccatcc cgctgcccgg gcctacttca atatcgagac agggcttccg gccgacgcga
     2881 tgcagatatt cttcctggag aatcccttca gagccgatac ggcggaagcg aagatcctga
     2941 gaactgccct ggatgtcatg ggtttcggga cggtcatgtc cgtcgtggac gctatgtatg
     3001 cgatcaataa cggcaacgta aaggaactct tctccctcct tttctcggag gccaaggggg
     3061 cgatcctttc aaaaacaggt gatatttcgg ccgctaaaaa acagatggta gatgcatatt
     3121 catctatcgc ccttgcggag aagatcgact tcgagatacg gaaggaccgt acggggccga
     3181 tagcggcgat ggaagagggg atattcgagt cccttcttga cgctttcggc cttgagaagg
     3241 ggcagcttgt tgtcctgagg ggaaggggag agcagaaact gtccgttaag acagaaaaag
     3301 gcccgaagca gatcccttca aaagagagag agtactttac agatgcggaa accgggatca
     3361 cttctttgag gtcgggggag tcggctttct tcataatccc ccgcgatatg accgtcgagg
     3421 cggggagtga aagtgaattc tcaagattct gataaggtcc ggaaaaacgt tcggcacttg
     3481 aaaaaaatca cctcagtcaa tggaggcaag agatgaagat ccacaccggg tacctctggt
     3541 tcgacaccaa aaacaggcgg gagatcgtgc cgatcacccg ggatgtagag aaaatcatgc
     3601 tggagtccgg gatcagcgag ggctttgtcc tggtgagcgc catgcatatt accagcggta
     3661 tcatagtcaa cgatgacgaa caggggcttc accaggatat ttgggcatgg cttgaagagc
     3721 tggctcccgc aaaaccggat tatcggcacc atcgtacggg cgaagacaat gccgacgcac
     3781 atctcaaaag gatcctcgtc catcatcagg caatgatacc cgtcacgaag ggcaagctgg
     3841 accttgggcc ctgggagcgg gtcttctatc tggaggctga cggccagaga aggaagcgcg
     3901 ttgtcgtgaa ggtcatgggg gaataggttc agccaaccct taccagcgat atttaaaaag
     3961 ctcttcgaaa cagatcccga tgaagatctc tttgtgctgc ctggtatccg gggttaaact
     4021 tgccaatatg gtatcgaggg cagattccat tggaggaaca cgatttgagt ccgtacattt
     4081 cgatccagga tctctcctac acccttccag acggtagggt gttattcaag gacatttccg
     4141 aaagcttcgg cggggagttc tcggcccttg cagggaggaa cggctccgga aagaccattc
     4201 ttgcaaagat catggctggg ctgatctctc ccggctcggg cagggtgatc cgctcgggga
     4261 cggtttgcta cctggaccag cttgtcgacc cctcggagta ttccaccgtt gccgatctgg
     4321 ccggcaagga aaagatcctt aattctcttc atcggataac cgccgggtac cctgaggaag
     4381 gtgacctgga gatagctgac ggcaactggg atatggaaga acgccttttg cgggaactgg
     4441 atatctccgg tttgggatat cttttgcccg atcttcccgc cacccgcctc agcggaggtg
     4501 agtgcacccg catctccctt atcggggcct tcctttcagg ggctgatttc cttatcctcg
     4561 acgaacctac caaccacctt gaccagccaa accgtaaggc actcatagaa tacctaggga
     4621 cctggaaagg cggacttctg gtcataagcc acgaccgggc ggtccttgag gtggtcgaaa
     4681 ggatatttga actgtcgtcg ggagggttgg acagctacgg ggggaacctg gatgttttcc
     4741 tcgaggcccg gaggctccag cgctctgcgg ccctggataa tcttgaaagg gtcaaaaccg
     4801 aacgaaaaag ggcaaaagcg gaacggagca tgctgctcga aagacagcaa cgtcgatcat
     4861 cagcgggacg aaggagcgct cccgacagtg gaattcccag gatagccatg aatgccatta
     4921 gatcaatggc aaaaaagacc gagggaaaga cccgctcagc ggctgcggag aaggtggagg
     4981 tactggctgc cgaggaaaag aaagccttcc tggaggcccg gatagaggac acggtggtgc
     5041 ttatcccgcc ccgatgtgcg gtccatgccg gcaagatcgt gcttaggctg gacgaggtgg
     5101 tccttccctt tggttctcac ggggaaccaa tcagccttac cgctgcgggt ccggagagga
     5161 tcgccgtcac gggtcccaac gggagcggga agtcgacgct gctgcgggtt ctcatgggag
     5221 aaacccgacc cctttcaggt tcgtgccgga tcatggtgga ctatgcctac ctggaccagt
     5281 tcacgtcggt acctttggat atttctccca tagagcacct ttgcgcagcc gaccccggta
     5341 tggagcaggg ggaggcgggg accaggcttg cgcaggtcgg catatcccgg gaaatgctcc
     5401 gggtcaagaa caggaacctt agtggtggag agcgtctgaa ggtggcgctt cttgcggcga
     5461 tacacagagt tcccgttccc cagcttctga tcctcgatga accgaccaac catcttgacc
     5521 tggactccgt tgaatccgtg gaaaaacttc tttgcgccta caccggggcc cttgttgtcg
     5581 tctctcacga tcgtaggctt ctggagaata tcgcgatctc cagggagctg gagatgtaag
     5641 cccgaacagg tgttcagacc ttatatgtgt cagcccgtac gcaattgaca agatgcctga
     5701 attcttcggc tgaagggagc gaaaaacctt tatgagtcct gatatgaatg agtctgttca
     5761 cggtgaatcg gccagccaat atgatgagga ggtcaaggcc tatagcagct atgggcacga
     5821 agtgatcttc ggaatggggt tcgagttcgt ccgcgaggga gaaaaacttc ttgatatcgg
     5881 aataggcact ggtctggcct cgataaactt ttccgggatc gggttgaagg tttacggatt
     5941 ggacaactcg cggaagatgc tggatgcctg tctatcgaag atgtttacgg aagaactggt
     6001 gctttgtgat gtgacgcgtg aggcaatacc ctatgaagat aatttttttg accatgtcct
     6061 ttgttgcggg gtgctgcatt tttggggtga tatgaaggcc ctgttttctg aggtagggag
     6121 agttatgaaa agtggaggga tctttgcttt ttcaattgcg ccccaggaga ccacatcgga
     6181 ttatatccat gagccgacag cctggggcgt gccaatattc aggcatgctc ctggttacat
     6241 tgcggagttg ctggaaacga acgggatgga attactgaag gagcagagat tgctgatcaa
     6301 gggtgcggat aaagttcatt ttgatatgtt gttctccgtc ctgatcagca ggcgcatctg
     6361 atatgccggg agaagcggag cgaaggcgtt gacagatgga gggcaacgga tcctatgata
     6421 tatactgata tcttcaataa ttcggccctt ggggagcacc cggcccgttg acggaacaag
     6481 agaggaagga cagccatgaa ggtatcaagg aaatccccaa cagcctcagt gcgtgaagca
     6541 tacccggtgc cgttccgttc gcttgccccg ttcctcctta ttacctttgg attggcctgg
     6601 agcattctcg ccttgtacat atttgcccag gaacctatga cagccatgtt cggccagctt
     6661 accggcaatc atccgctctt cttcctggca gtctattcgc cagcgatcgc ggcgtttgct
     6721 cttgttttcc gcaatggcgg ttttccgggc ctggggcgct ttctgggaag agccttgctt
     6781 tggcgctgca gccggggctg gtacgttttt ctgctggtcg gggttccgct tatcttcatt
     6841 gccggctcag cgatcagggg taaccttttt accgagccct tcccgtttgg ttcatttcag
     6901 gccctgatcc tggggttgtt cctcgcctcc atcaagggac ccgtcgaaga attcggctgg
     6961 cgggggctgg cgcttcctct tctgcaaagg aagttctcgc cgatctgggc cggacttatc
     7021 cttggcgcaa tatgggggct gtggcatatg ccggcattcc tcctgagcgg aacccagcag
     7081 agcgagtggt cctttgcggc cttttttgcc gggtgcctgg caataagcgt catcgcgact
     7141 tcgctgttca atggttcccg cggcagcatc ctgctttctg cgtttttcca tttcaccctt
     7201 atgaacccga tctttccgga cgcgcagccc tatgacacat acatgcttat cgttgctgcc
     7261 tgccttatcg tctggcggga ccgcaaggag atgttcacaa gggaaggctc cgtcacagaa
     7321 gtcattcccg gcggggtaga agcggccaat ccctgaccgg gaacctgtag gggtctacct
     7381 gcactttttt gcaataagcc ctgctctgaa aaaactggcc tgtttgacag gttcaccaag
     7441 tttaaggtct gaacctgtaa gagagggagg cacagaaata atcatggacg aaccaagtcc
     7501 ccgcttctgg gagatcttct tcgaggtttt tgagaacctg ccccgccagg gtcccggcag
     7561 ccgttcctgc gcagccagag cccttggcct ttgccgtgga ctgccggaat tccccgcgat
     7621 cctcgatctg gggtgcggtg tcggcggaca gactctacag atcgccgaac tcacttcggg
     7681 ttcgatcatt gcggtcgaca gccatgcccc gagcgtcgaa cggctgcgga cagccatcgc
     7741 ggagcgcggt ctttcgcacc gcgtcagtgc tgtcgtcgga gatatggctt ctccggagca
     7801 gccgcctgaa agttttgatc tggtctggtc tgaaggcgcg ctctacaaca tcgggctcga
     7861 gaacgctctt tccgtctgcc atgttatgct tcgccccggc gggtatcttg cttttaccga
     7921 tgcggtctgg cgcagggagg acccgctccc ggaggttaag gcagtcttcg atatggatta
     7981 cccgaccatg ggatggctgg cagacgatct ggccgcgata aaggatcgcg gattcgaact
     8041 tgccggacac ttcaccctgc ccgatgaggc gtggtgggac gatttctacg atcctatgga
     8101 ggttcgcatt gcggagctgc gcggaaaata tgccgacgat gccgatgccc cggccattct
     8161 cgacaaactc gccgaggagc cccgaatgca tcgcctctat tccgacttct acgcctacga
     8221 gtttttcgtg gcgcgccgtc ccgctgcagc gccgcggccc gtaacatcct gaaggcgtca
     8281 tcggggtttg aatatctcct cgcaatttag cgtctgaaga aaggaacagg ttatttcctc
     8341 aacttttcta tcgacgtctc cacacccgcc gggacaggtg cagccggcag ggcttccatg
     8401 tttttgaaga tccatttcac gtagttccag ttgaccgcta accgagtgac cttgatgccg
     8461 tctgaggtct ggttgaaaat acaatcggtc atcataacag aagcatttcg gaaactggcc
     8521 acggccctgt aactgttgtc aggccccagg tccatgatgt aagcgtcgtc tccgacgttt
     8581 ttaaccgttt cacccttaac cgttcccctt aaagcattcc agacttcctt gcttatttcg
     8641 gaggatccgt aaagggtcag atgaatgaca cagtgtctcc ccgatattcc gactccccat
     8701 cccttggtgt atgtagcact gagattatcg gactttgact tgtccagttt cggcttgaca
     8761 ccatccattc ccacctgagc agaggtcggg aagagagccc atatctggtc atcggtcatg
     8821 gttgcgaaag aagacacaac tggtttgaca aggataaggt tgtcgttcaa catttctatc
     8881 aggaccccct gatttttcaa ggcatttttg tgttccggtt ggtcaagaaa cttcgccagc
     8941 tgctcattgg acatttcctg ccccggtgtg cttccggcca actcttcgcc cgtaaccccc
     9001 agaaggttca caaaagtatc gcttccgctt gccaggccta tgaaagctgt tccctggttt
     9061 attttggcta ccgcatcgtc aagattcttg accatgggat cagggttctc attttcaatg
     9121 gtctggaggt cgttaacgag ggtcaatcca gcgcctgtaa aagaggctcc tgtggcaagc
     9181 ataagtcccg tcattcccgc tgctgttgtc acaaccggtg cggctaccac aagggccact
     9241 accgctcccc ccgttgtggc aacaaatttt gttcctgcca ttatgtttgc ccagtttttc
     9301 tgcccgacga cgttgccaat ttttgtgtga acattgccaa cagccccggt aagggaactg
     9361 atgccttcac cgatgtcttt tttcatcttt gaggtcacgc tgccaagagt gtgggccgcg
     9421 ctgtatatcg tttgcataca ggattcaaaa aaccctccat ggggccttat tgttgcgatc
     9481 ggagcgtttt ctgagaagga ggtgctgtcg gcagtagcat cggcaacctc tatacgttct
     9541 atggacttga tcgggtccaa aacagttgga tggaaagcaa catcgctttt tccggaactt
     9601 ctaatgtcag aattacttgc ggagtcagat aaagatgacg agaatatcac ctcggccatc
     9661 gcgggaatag cgaagtaatt ctgaaagaag agcgcgatct catgacgaag tgtcgggtca
     9721 tatctggagg cgatgcggat tatttcgatg tcgttcttga ccttgtttgc ggcgttttcg
     9781 gcagcgccga gggatgcatc ggcatatttt agtcttgcaa gaatctggtt gtccggaagg
     9841 tctttgccgt caatttcttt taacaggttg atagctctga cagcctgcaa tcctgtcaga
     9901 tgcataagat taagtgattc ggcaagacgt tctttgtggg atttagcgag ctccagagag
     9961 taaagaaggg gtttcccgga tgtttgttct gtttttgacg acgctgcctc tggttgggtt
    10021 tttgtgatag aaaccttgcc gccatccgcc ggtttgcttt cgaaggtgac cgccgaaaga
    10081 atttccgggc tcaaaaaggc caatatcacc gccgtggaaa aaaccgccag aatctttttc
    10141 ccttttacgg gcatgtctct cgacctccct gatgatcctt gactctttca atattttaac
    10201 atttcaagca ggcaaatgat ccttgtctgg ttcatgaaga aatcaaaaaa gtgctaccca
    10261 ttccttttca gccatatcta tgctgtttcc aaacctggca ggggcttttt cccttatctc
    10321 tgccatgatc ttgtgtttcg tagatgcaga cgggggctcc attacggaaa ttctcccgga
    10381 cctgcctctt atcaccagaa actttatcag cctgtgcgaa gatgcaaccc cggcggattt
    10441 tcgaggcgag aaagactctg tgactcttat ggtttccata tctggccatt tgacctctga
    10501 tttgatgaaa agccccttta gtatgatgcc gtcggaattt atctgcagga cctgggccga
    10561 catagcgctg gtagcgaggg ccagaaaggg gtttgcgaaa acgaccatga atgtacccat
    10621 cccccaggat atttcctctt tccattttgg agcgaccccg aaaaggaggg caaaagccat
    10681 gtcgaggaac catagagaga agaggacagc gaaccctgtt atcaccgtgt cccagataat
    10741 aacgtctctt ttcctgccga tgtaaatgcc ggatcctctt ttcgggtatg cactgaaaag
    10801 cgagtatagt cccgcaaaaa gaagaatgag gccggttatc ctgcggcctt cttcgaggaa
    10861 ggggatctct tcgtaaacct gcattcctgt caaagatatc cggccttcga aaattctgct
    10921 ccagagcaga aatttccccc cgggagcgtt cagggaaacg aacccttccc actcttccac
    10981 ggggaattcg ggtcgctgaa gtataggagt gtgttcccag ccaaacctgt ccattttttg
    11041 cgagatttcg tcaacaaggg ttttgagaga agggaaaccg gagagatctt cttctttgat
    11101 cacgggaagt ggagtctgtt ctccgttgat ctcccgtatc gttcctgtta cgcccagaaa
    11161 aagggcctca cgttcctgat aagagaccct ctgggtgcta atcggcaggg caagcagtac
    11221 aatcccgaaa gggataaaca gataagccag ccatcttttg aaccatatct tcatgttgtt
    11281 gcctcctgtt gagcaaccct tatgaaaaga aaaacaattg tactgatgtc atgataacat
    11341 gataatgctt aaggatctct tcagggagaa cgggaaagat gtttttgact ttgaaaagct
    11401 tgacagatta aaattcacag gtcggggcag aacagtggag gagcccattg ccgggaaaag
    11461 tgaggtgttg agaggtgaaa agggtccttt ttctttgtac ccataattcc tgccggagcc
    11521 agatggcgga aggctggggc aggcgcctga agtcagagac catggaggtc tggtccgcgg
    11581 ggacggaacc tggcaaggtg aaccccgtag ccgtaagggt tatgggggag tctggagtgg
    11641 atatctccag gcatcagagc aagcatgttg aggaacttat tcatatccct tttgattttg
    11701 ttattactct ttgcggtgac gcgagggata catgtccgat attccccgga gaggctacga
    11761 ggatcattca caggggtttc gatgatcctt cccgcgcgga agggacagag gaggaggtcc
    11821 ttgccttttt ccggagagtc agggacgaga tacgtgatta tgtcgaggga atgccggata
    11881 atattggcga ggaatgatct tttgagttct tcacgaagtt ggccccaggg ggtatgatgt
    11941 cagccgttgt gcccctcggt gcggttgtct tccgaagggc tatcccagag caaagtgagg
    12001 taaggctatg gacccccggg gaatacatta cattgtagaa gcatctggtt gcagcgaggt
    12061 tatcggcgaa gtggacagga tgcaggagat tctcgtggaa gcggccagga aggccaacac
    12121 ccacgtctgg gctgtatctt tccacaggtt tccccccaat ggggtcagtg gagtggttgt
    12181 catcagcgag tcccatcttt ccgttcacac ctggccggag gcgggttacc tcgcccttga
    12241 tatctatacc tgtggggccg caagcctgcc cgaagaggcg gtcaagcacg ttctcgaaaa
    12301 agttcacgcg gggcatactc acatcagcga acttacaaga ggtctggatg acgacgggga
    12361 caggatctat taccactccc tgataacctg ggaggaagaa cttcagaagg gcaagcccaa
    12421 aaaatgaccg ccataccccc tcaggcggtt acctctttcc ccggggcagc catcagcctg
    12481 cgaggtgtac ggaaaaatta tggaggagcc gaggttgtaa ggggggttga tctcgatatt
    12541 ccccggagcg agatccttgt tctgataggc ccttcggggt gcggcaagac gaccatcctc
    12601 aggatgatca acaggcttgt cgaaccttcc ggagggtcga tccttattga cggtgaggat
    12661 acatcgaaga tggatccggt gaccctgcgg aggcgtatgg gatacgttat ccagggcatc
    12721 ggtctttttc ctcacatgcg gatctcggcg aatgtctcgg tggttcccag actcaaggga
    12781 gttccaggtg gcaaggccat gatgctggcc gggaaggccc tggatctggt cggccttcca
    12841 aaggaagagt tcggggataa atggccccgc cagctttccg gggggcagca gcagagagta
    12901 ggagtggcca gggcgctggc gggtgatccg gaaataatcc tgatggatga acctttcggg
    12961 gcgctggatc ccatttcgag gatagcactg caggatgagt tccttgatct tcaggcccgg
    13021 cttggaaaaa ccattgtttt cgttactcac gacatcaatg aggcgatgaa aatgggcagc
    13081 cgcatcgcga taatgaggga cggagccatc gcccaggagg gagagccact gcatcttctt
    13141 tcttctcccg cggatgattt cgtggcggat ttcgttgggg ccgggaaccc tctttcgatg
    13201 ttcagcttca tgaaggtgag agatgttccc gtgaggtccg agggtcttcc tgtagttgat
    13261 attgccgcta cgatagatga tgcccgaagg gtggcccatg atacgtcact gaaattctgt
    13321 cgtgaccggt tcgtctacgt tgtagcggac ggaaggcttt gtgggagcgc cagggtcgat
    13381 aacccgggaa agagcgatgg ccatgatccg gtcctggata tcctggagac ggcttcgagc
    13441 gttcgtacga caaacaccgt tcaggaggcc ctggggctta tggtctcctc cgggatagcc
    13501 aacgtggcgg tgacaggtga gcgtgggaaa ttcctcggcg tgctttcatt cggtgacatg
    13561 gtcgacatca tccagcagca gagagaaaaa aacagtcgct agtcccttga cgcagcaacc
    13621 gggtgggcgg gctgaccttt cctttttatg aagtcctcga taagcttcct cgaaatgctt
    13681 atgctcggag ggatctccgg gaatgaatcg atcctgtacc agtccgcgtc ttctatctcg
    13741 acgccgtcga tctttatctc ccccgactcc cattctgccg tgaagcccac catcagagag
    13801 tgcgggaagg gccatggctg gcttccgaag tatttgatgc tcttaacctt gatggagact
    13861 tcttccatga gttcccgctc cactgtttct tcaagggttt cccccggttc gacgaatccg
    13921 gcgaggatgc tgtagcgtcc tttcgggaag cgggggctcc tggccagaag cagtgtttcc
    13981 cccttctcga cggccacaat gatagccggc gaaacggggg gatagctgat catttcgcag
    14041 ttggggcaca ttctggcggt ctctgccttg ctgtcttcca tcggtgaacc gcactgtccg
    14101 cagtactggt tctggcgatg ccactcctta agctgaaagg ctcttccggc ttcaaagaaa
    14161 gctttttccc ccagtattcc ccatatatcc ctgagtgaaa cgaactccat atctccgggt
    14221 atggggccgc attctccaac ttccagccag caattgcccg gcctttgtcc tgcggcggtc
    14281 tttccggata ttgtcgaaca gcccatattc ctgatttccg caatttgcgg aagtgagggc
    14341 ctgggtcctg ttttcacgag gatccggtca cccctgaaca cgaagcatcc ttcaagagcg
    14401 tttccgaagg tgtaactttc cattagtggc gctcctttcc tggatcgttg ctgcctaact
    14461 gtaaaaatta ctctcaacga gattaagcaa caggtctctg tgtaaaatag cagaaggagg
    14521 gtaatgatga aagaccttaa agttcacgcc aggaatgttg atgagcagac catggatcag
    14581 ataagggcca ttacttcaca cccggcggta gatggtcccg tctcgataat gccggactgt
    14641 cacgttggtg cggggtgcgt gataggcttt acggggcgat tcagggatgc agtgatccca
    14701 aacgtcgtag gagttgatat cggctgtggt gtcatgctct cgccaatgga tggtgttgat
    14761 agagggaacg cgcgtttcgg ggaccttgac tcccatatca ggaaagtggt cccactgggg
    14821 cccaaccatc acggaggaag gtctgtcttt gatcgcttcg gcggacgttt tccccgtcga
    14881 gccgggagaa tggaggaact tgcgggtctc gcagatgatt tctacgggag gatcctccgg
    14941 aacagaacgt cggcccctgc actcacccaa ttgggaactc tcgggagcgg gaatcatttt
    15001 gtagaggttg acgaagacgg agatggccat ctgttcctgt ctgtccacac cgggagccgt
    15061 aacttcggct accaggttgc aggatatttc caggatatgg cgaggtcttt tctccgcggt
    15121 tcgggccttg gggagggtat tccgcagggt ctggagtatc tccccatgga cagagggggg
    15181 agggattaca tgaaatggct tgcagttgcc caggagtttg ccgaacataa ccgtttcatg
    15241 atactggact cggtccttgg gttctatgga ttgaagcccg acatggcttc catcaccgat
    15301 agtgtccata actacatcag cgacagggac gggatagtcc gaaagggagc tatttcggcc
    15361 cggaatgggg aaaaggtagt gatccccctc agcatgtcct cgggaatagt aaggggaaca
    15421 ggcagaggta accccgcgta taactgctcc gctccccatg gggccgggcg tcttttcggc
    15481 cgcaaggaga tgaaaagaag gctcagtgac ggggacatat ccatggagat gttccagagg
    15541 agcatgaaag gtatattttc aacgtcggtc tgttccgcga cgatagatga atcgccaatg
    15601 gcctacagga attgggagga tattcaagaa gaggttgcgg agacggtgga tgtatccgcc
    15661 atactgaggc ccgtctataa tctcaaggcc gcgggataga aaaaagggcg gacacattgg
    15721 cgtccgccct gaaaaaactg gtagccccaa cgggattcga acccgtgtta caggactgag
    15781 aaccctgggt cctagaccac tagacgatgg ggccacttgt aaatctggct gggggacagg
    15841 gactcgaacc ccgacttcta gatccagagt ctagcgtcct gccaattaga cgatccccca
    15901 aggcaattcg ctccccgagc gcgacggata tattatcacg ggtcaagtgg agtagtcaat
    15961 gacccgggat caggattctg gccggaatca ggacgaaaga aagttcaggg gagtaaaggg
    16021 ccattcttga gtgtacaatg aatacagaca attactggag gtgttcgcgg tatgaacgta
    16081 gacagcagat cgttccggga acttctggag aaaagacctt tgaacgtaca ggcggctttc
    16141 ggtaccgaac ctcttgccct tgtcagcggc agggacatag cggccgcagc aaggaagacg
    16201 gggtcgatag tcatggccgc caatgtcagg aaccccctca ccataaaggg aattctgaag
    16261 gccgcaaaag agatggacgc ggtggtgatg ctggagctgg ccaaatctga agcgacttat
    16321 tgcggctgca cctttgacaa tgtgccgggt cttgccgcac gctattcggc ggaactcggg
    16381 ggaggtgtcg ttttcggcct ccatgtggat cactatgcga tcaagtccag ggatgatctt
    16441 ctgcggtcca tcgctcacct gaggcaactg gtggagaatg gctggacatc ggtcgcgatc
    16501 gatgcctctc ataacccgga ctgggagaac ctttgcttta caagggatgt agcgatgcat
    16561 gtccccccct acctgggact tgaggcggaa gtgggagaga ttaagggccc cggcgagata
    16621 acgactgtcg aggaggcgct cttcttcgtg ggaggactca attcatgggg ggtgttcccg
    16681 gattttcttg ctatttcaaa cggcagcaag cacggaacct acgacagttc actcggggag
    16741 gaggagggta tcgatctttc caggacaaag gagatcgctg aatcgatcgc tcccttcggg
    16801 gctgtgatag cgcagcacgg aatatctggt acacctctcg acaaggtgtc cagcttcgta
    16861 aactttggca taaacaaggg caatgtgggg accctctggc agaatatcgt ctttggcctg
    16921 gagatggatc cgtctacggg taatgccctg gtccgggacg acgcctacgt caagaataac
    16981 agccgaggcg ttcctcccga actgtgggaa cggatggtca catgggccga tgaccagggt
    17041 ttctccagga aatccggtga ttacaagaag gccaacaaac cctttcacaa tgagatcatg
    17101 gctcttccgg aaaaaatcag ggagagcatt gtcgaggata cggccgattg ggccaggcgg
    17161 ttcatcaagg cctttaatgc cgaagggagc gccgaggccc tcctggaggt ggtatcgaaa
    17221 cgcccggatc ataacgccgc ccccgaaagg gaaataatca gatccaggag cctctttacg
    17281 aaggagagcg ctccgggaga tgttggaaac agagacggga aagactacag cgattgagcg
    17341 gaagatattt gcttacatca acaatactgt tcggcaggac ttttttccgt gggactaagc
    17401 cgccgggacg tggtaggata gtcaaccgga ggtgaaatga tggtggatac aaggtcgcgc
    17461 gaaaggcgcg gcaggaagaa aaaaggaaac agtgaactgc gcttcatccc cctcggaggg
    17521 cttggacaga taggtaagaa tatgttcgtc cttgaatacg gggaggatat tatggtcgta
    17581 gactgtggcc tcatgttccc cgatgaggaa atgcttggaa tagattttgt aatacccgat
    17641 gcatcatatc ttttcgagaa cagggaccgg atacggggga tggtcctgac ccacggacac
    17701 gaagatcata tcggcagcct tcctttgatc ctcccgaggc tggatgtgcc aatatatgga
    17761 acaaaactga cccttggcat ggttgatgcc aagttccgtg attcttgtcc cgactacact
    17821 ccgaagtcct tcgagttttc agccggtgat aaattcagcc tcggatgctt cgatattaca
    17881 ttcattgccg tgtgccattc cataccggat ggagttggac tggccattga gactccggtg
    17941 ggaacggttg tccatacggg agatttcaaa cttgatccga ccccggtgga cggaagaata
    18001 accgattatt cgaccttctc tgaattcggc agcaagggcg tactgctcat gctttcggat
    18061 tccacaaatg tggagcgacc cgggtttact cagtccgaaa gaataatcgg caggaccctg
    18121 gaaagattgc tgcggcttca caggaacaac cgtcttgtta ttgctgcctt ttccacaaac
    18181 cttcacaggt cccagcagat ccttcaggta gcttcacgtt tcaatagaaa ggtcgcgctg
    18241 atggggagaa gcatgatcca gaatgtgacc ctcgccattg agctgggcta catagatgtg
    18301 gacaaaaaca tactcgttcc gatggaggat gtcggcaagg ttcctgacaa ccggcttata
    18361 gttcttacta ccgggagcca gggtgagcct ttttcgggcc tggtgaggat gagcaaggga
    18421 gaacacaggt ttgtcaatct cggccagggg gacgtggtgg ctgtttttgc ctctcccatc
    18481 ccgggaaacg aaaaacttgt gagcaggacg atcaaccggc tttttgaatg cggatgcgag
    18541 gttatctatg atcgcgacca ggaagttcat gtttccggtc atgccgccag agaagagctc
    18601 aagatgatgc tgagcatggt aaggccaaaa tactttgtcc ccatgcacgg agaataccgc
    18661 cacctcgtaa gacacgccag gcttgcgcag gagatggata ttccggcaag gaacaccttc
    18721 gtccttcaga atggagacct tctttcgatc aatggaacaa aggcccaggt aaaaagagga
    18781 gaaatcactt cgggagcgat cctggtcgac ggcatggctc ttggcgagat gcaggacagt
    18841 ctcatgagag agaggcagga gatatccgaa gaaggcctgg tggttatctc gataaccctg
    18901 gacagggatc agaggcttat cgctccgccg ctgatcgata gtgtagggtt tattcatatg
    18961 aatgacgcag gggggcttgc gaaagacctc caggatgcag tgatggagtc cgtggcccag
    19021 ttgcatgaag gcaagacaca ggatatcgac aaggtctcga acagggtaag aaacagggca
    19081 aggagtgtac tgaaaagatt cagccgtacg ccgccggtta tcaagatcat gataacccgg
    19141 gccggttcct gaaggagccg gttttttcag ggaaagtgac gtttttgaat cgggggagat
    19201 gcagtcttgt caactgggct tttctttaac ttgctgggag gggtcggcct ctttctgttt
    19261 ggtatcaaac tgatggggga gtctcttcag gatcttgccg gggacagaat gcgccgcctg
    19321 atatctgccc tcaccagttc tccggtaaag ggaattttgc tgggcgcgat cgtgacggtt
    19381 ctgatccaga gcagtagcgc gacaacggtt atgacggcga gttttgtaca tgcggggttg
    19441 atgaccctga agcagtcgat aggcgttatc atgggagcat ccattggaac aacggtaacg
    19501 gcccagataa tcgccttcaa gataaaggag tttgccctgc ctatcatcgg gttcggcatg
    19561 atctttgccg tcttcgggag gaccaagaga cataaataca ttggcaatgg catagtgggt
    19621 ttcgggctcc ttttcgtggg gatgcagacc atggagagcg ctatgtactt cctccgcgac
    19681 aggcaggata ttttcctggc gttccagcat catccgatcc tgggagtcct ggcgggaacc
    19741 ctgctgacca tgttgatcca gtccagttcc gcgacggtgg gtcttaccat tgttatggca
    19801 tcccagggac ttcttacgct ggactcggcg ataccgatac ttttcggcga caatatcggc
    19861 acaacgataa cggccgttct cgcttccatt ggcctcaaca gatccgcaaa gcagtccgct
    19921 ttcgcgcacg tttttttcaa ggtaattgga gtgatcgtgt ttctgctgtt ccttgaacct
    19981 ttcagacatc tcgtaacgct gacctcctcc gacatcgcca ggcaactggc caacgcccac
    20041 acaatattta accttttgaa tacccttatg ttcctgccct tcatcaaccc ctttgtacgt
    20101 cttgtaagga ccgttcttcc cgatgacggg gtgctcttta tgatggggcc gaggtttctg
    20161 gacaagaacc ttatttcggc atctccgata gcggcagtgg aggcggtaag aaaggaactt
    20221 gtcaggatgg gctttctgac gctggagatg atccggcatg tcaagaaggc attcgtggat
    20281 agcgacagaa agatgataga acaggtaaac cagactgagc gctgcgtcaa tgacctgacc
    20341 agggagataa cggcctattc cgccgacctc atgcaggtca gccttccacc agtgctctcc
    20401 tcttcactgt ccctcttcgt aaacggtgtt ggtgatattg agagagtcgg agaccacgcg
    20461 gaaaacctta ttgaactttt cgaatacaag atcgagcata accttccctt ctcggatctc
    20521 gccatggagg agttcaacgc gatgatatcc atggccgagg aggccttgct ccttgcggtg
    20581 gaggctctgg agacatccga tggagataag gtaaaaaagg tcttcagact ggaggacaag
    20641 attgacgcca tggagcgggg ccttcgaaag aggcacatag aaagactcaa ccagggagtc
    20701 tgcaacccgg gttcgggggt tatcttcata gatatcctca gtaacctcga gagggtggga
    20761 gatcacgcca acaacattgc tctcatagtc aaggatatca acctggtaag aggcaaggag
    20821 gaataggatg ttgatctccc agtgcgcgct ccttggtctt ctccagggat ttacggagtt
    20881 tcttccagtg agcagttccg gacatctggt aattgcccag tccctgttcg gctggcagga
    20941 ccccgcgata gcctttgatg tgatgttgca tcttgctacc atggcggcta cacttgtttt
    21001 tttcagaaag gagatttttt atttagccct gggatggttc ggaggactgg tttccagagc
    21061 cggaagagat catcccggct ggaggtacgg gtggagtgtc ctggcaggtt cggtggctac
    21121 ggtggcgatt gttctggtga cgaggcccgt tatacatgtg tttttcggca ataccctgtt
    21181 tgtagggtta gcccttgtct ttacaggtgc ggttctatgg tacggaaacg gtatcagtgt
    21241 caaagatggc gaagtgtcag taccgacggg gctgatggtt ggacttgcac aggggctggc
    21301 ggcatttccg ggactttccc ggtccggcct gacgataatc gcgggcctga agtccggttt
    21361 gggacgcgaa gaagcctttt ctttctcttt cctcctttcg cttcccgcca ttttgggagc
    21421 ggcgatcctt gaactccccg atgccggagg gatatccgga gtttccggct cccttccgca
    21481 ggggtggtgg atcggggtgc tccttgcttt tctttcgggt cttttcgctc tttcacttct
    21541 ccgtaaagta gtggtcaggg ggaaatggaa ggtgttcgcg gtttattgtc ttatcgccgg
    21601 agcgtttgtc ctggctgtca atattttcgg gaggtgatga agatggccag gtccgggggg
    21661 tcgtcaaggt ggtggatcct tatcctctgc gtcattctgg gaagttttct cggggtttat
    21721 ctacagggtt tccaggcgac ggagaatctt ttccgcaatg tagtcaacat cggttttgat
    21781 atcggcgatg tcgatgtcct tattgcccgg tttggtttcc ggctgttttt caggctgaac
    21841 cttgggactt tcatcggtgg ggtcataggc gtgctgatga tcagatgatg atagaccaat
    21901 ccttcccgca agatgtagtt cttgcatccg caagtcctcg cagggctgcc ctcctcggcg
    21961 atctcgggtg gtctttccgt attgttccac ctgatatcga tgaggtcgtc agcggggggg
    22021 aatctccctc tggtctcgtg gtaaggcttg cagatgagaa agcggaatgt gtctgccgga
    22081 aatgtccaga tgcactcgtg atctcggcag atacggttgt agtccttggt gatttaatac
    22141 tcggcaagcc agagaatgcc caggatgcgg caaggatgct ctcaatgctt tccgggaaaa
    22201 ggcatacggt catgaccggg gttgcgctga ggcacagatg tgtctctgtt tccggatttg
    22261 agtctacgga tgtttttttt cgagagcttt cggagcagga gatagagaga tacgtaaggt
    22321 ccggtgagtc ccttgacaag gcgggagcct acgccataca gggacggggt tcgcttcttg
    22381 tcagttctat cgaagggtgt tatttcaacg tcgtgggcct gccccttttc aggctgagca
    22441 gactcctcga ggaggcaggg atatctcttg agcagcagtg gaggtgaggg gatgagagtc
    22501 aggcagtctt ttgttcttgc ggcagggatg atggttgtag caattatcgg tgccctggga
    22561 cttgtaccgc gtatatcggc agaaagatca ggaagcgtgg ctgctcttgt cgtcgaaatg
    22621 agagatgttg ccaatatggc cagggaggcg ggtcttcctg ttgagcaagc agtcgaaaga
    22681 ctcgaggctg aagggcttac ggccctggct gttggagaac ttacggggca ggaacttctg
    22741 tccgggatgc tcccggtgga gtttgattcg gcccgcaacc ttatccacgg tgatcttccc
    22801 gagggagttt ggccggattc tgcggccctt ctcttttcag aaaaagacaa acttgacgac
    22861 aggataaggt tttttgtcca gacccggttt ccgggagtta tagaagtaga taaagatgaa
    22921 gacctgttgc tggttcttcc cttttccctt tcagagaccc tcgaagccgg catcctgccc
    22981 gattatcctc tactcgaaat ggcctcaggg acagggatgc ctctaatata ccgtcccggt
    23041 tcgacgccag gtatctcggg tgattctgta gcagattcag ttgaaattgt tctcgacggg
    23101 ttcccgaata tcaggagcgt cgtcccggcg gggctctttg tcgcgggtta tccggaactt
    23161 ggacctctgt ccaggctgct tggagaaaga gagatctctg tggcaaaggt ggagttctca
    23221 cgccagatag gtgtcggttt ccttgagagg gccctgtttc caaatgtcct ctccctgcac
    23281 agcgtccgca aggaggagat agtaagccgc agactgacga gggacgatct tgtcgaacgc
    23341 tttgtcaggg ctgcccggga acgttcggtg cagttgcttt atctcaggcc ctctgatctt
    23401 ctttcgggat ccaggctgga ccgtttcgca aatgagtgtg gaaggatctc tggacgtctc
    23461 gaaaaactgg ggatcggcaa cggttggccc gaaacgcttc ctttgtggag gagcggtctt
    23521 ttctctgctt ttgcctgcgc cttggccctt gttcttcttt cgatgaggct tgtttcgaga
    23581 tacctgggcg cggaaaggga tttcgggacc aaaaccctgg tagcgctgtt ggggctgtct
    23641 gtggttctgg cttttttgat gttgaagata tctcctgtga tcaaactctc aggggccctt
    23701 ctcgcggctc tggccgccac cgaggcttcg ctcctcgcac ttgagaattt ccgaaaaccc
    23761 gtaaggggag tgctcatgtc cgtgggggtg gcagtagcat ccgggcttgc catagccggt
    23821 tttttcggca cgccatggta tatgcttcga atggatgctt tttccggggt gaaactgacc
    23881 cttcttcttc cgccgctttt ggtgctttta catgacctca agaggcgtgt tcacccggaa
    23941 tcacttgaaa acgtcctgtc ccgccccccg cactggggcg aactggcact cataggaggg
    24001 atccttgctg ctgccgggct tatggttttc cggagcggca atgtttcgtt cgttcccggg
    24061 tgggagatcc gcttcaggga acttctcgaa cagatccttg tagccaggcc aaggaccaag
    24121 gagatcttcg ccggatatcc tgctcttgtt ctctggttca cggtgagacg catggatatc
    24181 tggccagggt atcgtgagat attcaggctg ggcacgaccc ttgccttttc atcggtggtc
    24241 aacagtttct gccatttcca tacccacacg acctttatat tcctgagagt ttttaacggt
    24301 ctgtggacgg gtttgctcgt aggcctcgtg ctttcggcca ttctcgtgtt tctcgtcctc
    24361 ccggtctggc gcaggtggcg ggaggtcctc gcggggtgaa gcgccccctc gatgtgacta
    24421 tttgcggctt ctacggtttc gggaaccttg gggatgaact tattgcccat ggactggtaa
    24481 gtctcctgat aaagaacggc attccttccg accgaattgc cgtgctgagc gcatcgggca
    24541 atatcgaggg cagttatgga acagttttgg ttcccaggtg ggatccattg aaagttttta
    24601 aaaccctggg acagagcagg accctgcttc ttggcggggg cggactgttt caggattcca
    24661 ccagtatccg ttcctgcatt tattactggg ggatcgtgag aatggcggtt ctggcaggat
    24721 gcatcccgtg ggctttcgga cagtctatcg ggccgttaat aagcggcgct gcttcatttt
    24781 ttgcccggga tgctctttca ctatgcaggg tcaggtgtgt aagggacgag ggttccctgg
    24841 attggctgaa ggccaatggt ctggagggaa atatcgctcc ggatacggtg ctgacccttt
    24901 cggatcggat ggatatcaac ggtggcggca gggatcacct gcttgtcaac atcaggccct
    24961 ggcccggttc tcttccagcc acggcggcat ctgccgccag gatcctgggc gagaggaccg
    25021 ggttgccttt aatgggtgtg gctctttctg atgatgatct ggaagaaatg aaaagactct
    25081 ccggtgacgg gttgttctcg cccgttgcca taaggagggt caggacattt gacgatgttg
    25141 tggagacctg gagattcggt tccgaggctt ttgggatgag actgcatttc tgtgttctct
    25201 ccgcaatggc ggggatgccg tgcgtggccg ttccatacga tcctaaagtt tccgggtttg
    25261 cgtcccgttg ggggcttcct gtatggagtg gagagggccc ccttcctgaa atggttatgt
    25321 gttccggcac aaaggaaaag gtgacagaat cgagttcaat gatcgaagag tcctttgcga
    25381 aaatgctcgg gaccgttctc ggcggtgagt gagagagatg gatatttccg aaaggcggaa
    25441 cctggagaga aaggctgttg aggcaaggaa agatgttgta aggatgacgg gtgttgcgag
    25501 gtcgggatac ctggccgcgt ctctttcggt tgtagatatc ctggtctttc tttactggaa
    25561 agaaatgaat attcggcccg ctgaacctct ctggcatggg agggaccgct tcgttccggg
    25621 caaaccccat gcatgccctg ctctttactc ggtcctggcg aacaggggtt tctttgacag
    25681 gaaggaacta tggaatttca ggcggctggg ttcgatgctc cagggatatc cggaaactcc
    25741 gaggaccccg gggatagatg cctcgggagg ttcactgggc atgagtctcg gcatatccaa
    25801 cggcatggcc cttgctttgc gccttgacgg atcagaacaa agggtctttt gtctcatggg
    25861 cgacgtcgaa cttcaggaag gatccgtatg ggaatcggtc atgcagacag caaacctggg
    25921 cctggacaaa gttactgtaa tagttgacat gaatagttcc gggatgcacc agggagagga
    25981 ctctgttaaa gagatcgagc ctgtaagaaa caggttcgag tcttttggat ggcgtgttga
    26041 agaaacggac gggcacgata tggatggcct tgaatccgcc ttcgccggga ctcttgccaa
    26101 aaactcaacg gagggcattc cctgcgcggt gctggctcat acgatcccgg gaaagggttt
    26161 ttccctggcg gagaagggct gcatcggccc gctggaggtc cttgacagga acatgatgga
    26221 gcaggcgctg cgggagatgg aatcatcctc tgctgttttt ggaaggggtg aagatcattg
    26281 accagtactc ttgaaggttt tgcagagggg cttgttgact tcgcggaaaa gaatagagag
    26341 gtggtccttc ttcatacgga cacggccgcc tcatgcggtg tgtcgatctt tgaagagaag
    26401 tttccggaaa ggagtttcaa ctgcggagtt tccgaacagg acatgatcct gacggcagca
    26461 ggactatccc taaccgggaa gatacctttc gcctgttccc gtgccgcctc catggccccg
    26521 agggcctttg accagataag ggcgagcctg tcgatcccgg gtctttcagc ggttcttgcg
    26581 ggaacgtttt cgggcctctc agcgggagaa gatggagctt ctcaacagat gaaccaggat
    26641 atcgcgataa tgcgaagcct tcctcaaatg accgtcctgg ttccagctga tgcctcttcg
    26701 gctttcagta tggtttctct cggagctttg aatcagggcc ccgtgtatat ccgccttggc
    26761 tcggaaaagg tcgaagcggt agaggcgatg gctggttcgg agatgaagat cggaggggga
    26821 cgattgctcc gggaaggcga cggagtcact atatgtgcct gtggtatcat ggtgcacgaa
    26881 gccttaaagg cggcggatat ccttttcaga cagggtatca acgccgaggt cctggactgt
    26941 ttcagcctga agcccctgcc tgcccccctg atactggcct ccctcagaag aacggggtgc
    27001 tgtgtgacag ccgaggagca taatgaggtt ggaggtcttg gtgaagccgt ttcggcgctc
    27061 tgtgcaaaaa agaatcccgt tcctgtcagg tctgtagcca tcggggacaa gtacggacag
    27121 agcggcaagc cccccgaact gcaggagtat tatggcctta ctttcagaga gatcgtcgga
    27181 gaggccgcac aggtctggtc gatgcgcagg aggtagagaa tggagatccg cttcgccgga
    27241 gtcacaaaac agttcactcc cgacatcgta gccctcaagg atgtttttct taccattgcc
    27301 aagggtgagt tcgtttacct cgtaggcccg accggttccg gcaagacaac gcttctgcgg
    27361 ctggttacaa gggaactaat ccccaccaag gggcaggtaa ctgtgggtga caccaacatc
    27421 aggaagatcg cctcctacga tctggcctac cttagacggg atatcggagt ggtgttccag
    27481 gatttccggc ttttacctca cctgacggtt tatgagaacg ttgcttttgt gcaggaggcg
    27541 ataggggtgc ccccatcgga tgtgcagaag aggaccaatg atgttctcga cagactgggc
    27601 ctttggcgca gacgctcact tttcccggag cagatctcgg gaggcgagca acagcgtgtc
    27661 gccataggga gggccattgc caactcgccc tcgatcctgc tggccgacga gcccacgggc
    27721 aatctggata tccacaaggc agaggagata acgcaacttc tgctttcaat taatgcctca
    27781 gggacgactg tcattgttgc cacccataac cagtatattg tcgatgccta caggcagaga
    27841 gtggtcgagc tgcgcaaggg ccttgtcaaa agagacgaaa gaaagggaag gtacgaggtc
    27901 gatggcgacc tttaactaca tactgaggga cacatggaga ttgctttcca gacactgggg
    27961 tctgagcgta ttgacactgc tcacctctgt ggccgtgttt tttctggtgg gtgcgagcgt
    28021 tctttttgtt ctcaacacca ggtatcttgt ggccgctgtc gagggtgacc tgatggttca
    28081 ggcctatgtc aagaatactc ctgaggctct ggaatccgtg gcaaggaagg cctcatccta
    28141 cgattcggtg tcttccgtaa acataattac atctgacgaa gcgctggaaa agctgaaagc
    28201 gaagcttggc aagcaggccg aggcggttac cctcctggac gagaaccccc tgcctccaag
    28261 cgtggaggtc aaggttaaaa gagctgcctt tgcctcggtg atcgcaagag aactgatctc
    28321 catgccggag gtcgaggatg tggtctactc cggggcgata gcggacaggc tggagcgtat
    28381 atcatcgttt gtgtcgaaac tatcggtgat cattcttgct gtatcgatgg cttccgcctc
    28441 tcttgtgctt ttcaatacca taaggatcag tgtatatgcc aggaaggaag aaatctcgac
    28501 gatgctcctt gtgggggcca ccagaaattt tgttgtcttt ccctacgttt tacagggtgt
    28561 agtgcttggg acactcggag cccttttatc ggctcttctg atctggtttt catacggggc
    28621 ggctatagaa gcgcttgaaa gatctcttcc ctttcttcga cttatttccg gaacgggact
    28681 cgttcttcga ctcgggctgg tcctggtttg tgccggtgtc actgtgggat ggatgtgcag
    28741 ctggcttgcc gcctcgcgtt ttatcaggct ggcatcgagg tctcactaga ccccgatggg
    28801 gggtgctgat aatgaagttg aatagccgtt tcctgacgtt gctgatcctt ctgtcggttt
    28861 tttctctcta ttccggagtg gccgtggcat cagagatcga ttcgaagatc aggaccgagc
    28921 agaaccgcct ggaggcgatc gaaaagcaga tcgcttacca tcagcgacag atagcggatt
    28981 cccggaagaa agaacagggg ctccttgacg aactttcgaa gataaaccag aatatcaccc
    29041 ttctgaaaca gaaggtagcg gtgcttgatc tccagaggga aaagacggag atcaggatcg
    29101 gggagctcga ctcggagata gaaaagaccg tgtcggaact ggaaaaggcc aaaagatatc
    29161 ttgccggcag gttccaggcc ctgtacaagt atggtggtgt cgcggagttc aatctgcttt
    29221 tgtcggctgc cagcgctcac gaggccatga acaatgcctt tcttctcgga aggatagccc
    29281 gtgaagatga aaggataatc agggagactt cagagagccg aaaaagactc gaggctgcaa
    29341 ggctggagat gggagagcag aaaacactcc ttgataacca gaggaaatta cttggagaac
    29401 agcagtcgtc gctgaaaaat tccgagacac aacggaaaaa atccctcgat actcttttca
    29461 ggcaaaaaca actgcatgag aaagctgcca gggaacttga ggaatcccag aaagaactac
    29521 agaacaaggt gagggaactt cttgcccaga aaagaaaaat gcaggaaaca agggcaggtg
    29581 tggttacgcc ggtcttcccc acgggcggca agttggcctg gcctgtgagg ggagacgtaa
    29641 ccagcaagtt cggctccagg gttcacccgg tcttcaagac caagatgatg cacacaggag
    29701 tggatatcag ggctggagag ggaacacccg tcaagaccgc tgctccgggc gaggtgcttt
    29761 ttgccgggtg gctccgggga tacggacagg ttataatcat cgatcacgga agggatctaa
    29821 cgaccgtcta tgcccatctt tcgcagatca gggtggagga gggcaagatc gtcaagaatg
    29881 gagagatcat aggaaacgta gggagcactg gggttacttc gggaccccat ctccattttg
    29941 aggttcgcgt taacggggat gcgaaggatc cccttggcta cctggcaaga taactgaaag
    30001 ataacgaggg gcgggagttc ggcaatgatt cgacgggtgc gggatattct tttcggggcg
    30061 ataacgggag cgatccttgc aggctgggtt ttttcagctg gtgccgggag cggaggggat
    30121 gttttcagcg cgtccccctt tggtttccag gatctgtggt tgatgaagca ggcgcgtgtg
    30181 atcgtcgaga cttaccatgt cgaaggttta aaagatgtag atgagagagt actcctgtat
    30241 ggagcaataa gagggatggt cgctgccctt gacgatccat acacccgttt tgtcgatcct
    30301 gatgaactgc acgaggaaga gatagagatg cagggggaat acggggggct cgggatttat
    30361 ataggacagc gcgacgggag gacccttgtt ataagcccga tagaggatac tcccgccgac
    30421 agggttggcc tcaaacccca ggaccaaatc gtcaagatag gagaagaggt cattctgggt
    30481 tggacccaga acgaggtggt caacaacctt cgcggagagc cggggacggg tgtgaccata
    30541 tggattcgca gggaaggcgt ggaagatctt ctcaggttcg acatcgttcg agaaaagata
    30601 tccatacaga ccgtcaagtc ggagatgctt cctgacaaag tcggcctgct caggatcatc
    30661 cagttcaacc agaagacggt tcctgatttc gggacagccc ttaagaaact ggaagagcag
    30721 ggagccaggg gttttgtcct ggaccttcgc aataaccctg gaggacttct caacagttgt
    30781 gtcgatattg cggacatgtt cctggacaac ggggtgattg tgagcaccag gggacgtttc
    30841 gacagggcga atgaggtcct tttcgctcgt ccagggatga tgacccgcaa gccggtagtg
    30901 gttcttgtca acgagggaag cgccagtgct tccgagatac ttgccggggc acttaaggat
    30961 cagggaaggg cccaggttgt cggcaaaaaa acctacggaa agggttcggt ccagacactc
    31021 ttcaacctct ccgatgattc cggagttttc gtgaccatag ccaggtactt caccccctcc
    31081 gggacaatga tagataaggt gggcctttcg ccggatattg aggttgaggg aggtccggag
    31141 aaggaaaggg aaaaggacga acagcttaac agggcaataa aagaactgct gcttatagcc
    31201 cgctgatcgg ggaatgataa gaatgtcccc cgggagattg aaaagaggtt ttattctcct
    31261 gatcgtaact atgggggttt tctcacacat gcccctttcg aatgggtggt gcgatgtttc
    31321 ccggccagtg atcgccgtga tcgtcgatga tctcggttat tccgtcgaga tggcccggga
    31381 tcttgcgagg atcccattgc ctcagacctg ggcaataatc ccataccttg ccgagagcag
    31441 aaggacttcg gagatagccc tcgataaaga tataccgttc atgttgcacc ttccgatgga
    31501 ggctgttgga gatgacggca agaggcattc tttagtatgg gttggaatgt ccgaagagag
    31561 catcaggcag gctgtaagga atgccctgtg gtctttgcct ggggtttcag gtcttaataa
    31621 tcacagaggg tccaaagcat cgcaggatcg tggggtcatg ggggctgtcc ttcgggagat
    31681 agcggctgag ggcctttttt ttgtggacag cagaacgtcg ggagattcgg tgggttttcc
    31741 cgttgcggtt gagatggggg tgccagcgac ccttaacagg agctttatgg atcatgaaga
    31801 caacgaaggt ttcatgtgga gccagttccg caaggctgta gggatcgcca gaaagactgg
    31861 cggtgcagtg ataatatgcc atgcgaggcc ggggacactg aaatttcttc cgcgactgta
    31921 cgaaaaggcg cacgaggatg taaaatttgt gaccgtgccg gagtatctgg agcaaaaaag
    31981 aactggcggc tgggaggagt aaccggatga agggtcgggt agaagcaatc atcggagcgc
    32041 aatggggtga cgaagggaag ggcagggtgg tcgatgccct tgcttcccgt gtggatgttg
    32101 tcgcgagata ccagggcgga gccaacgctg gacatacagt gatcgtcgaa ggagaaaagt
    32161 atgtttttca tctccttccg tcggggatgc tctactcggg caagacatgc gtcataggca
    32221 atggtgttgt ggttgacccg gagcagctcc tgaaggagct gaaggaactc caggacaggg
    32281 gcaaggatcg tgcccgcctc gtgatcagcg gggctgccca tgtcgttatg ccctatcaca
    32341 agaccatgga tggcatggag gagcgcctga gaggttcggg caagaagata ggcacaacac
    32401 agaggggcat agggccctgc tatatggaca aggtcaacag gtgcggaata agggtggaag
    32461 atcttctcag cgaggatgtt ctccgcgaga aactttcatt caacatggaa atcaagaaca
    32521 tgtaccttac caggatcttt gacgagaacc cgatggcatt cggagaggtt tttgaggtgg
    32581 ccagaggctg gggcgaaaaa ctggctccat atgtcgggga tgcttcactt acgatctcca
    32641 atgctgttga gtctggcagc aatgttcttc tggagggggc gcagggcacg ctattggacc
    32701 tggatcacgg cacatacccg ttcgtgacaa gttccagtcc cgtcgcgggc ggaggaagtg
    32761 ttggctgcgg attagggcca tcgatgatcc atcgtgtcac cggggtggtc aaggcctatt
    32821 gcactcgggt gggcgaagga cctttcccga cggaggatac cgatgtggac ggcgagaccc
    32881 tccggaacag gggcggagag tatggggcca ctacaggaag accaaggagg tgcggctggc
    32941 tggatcttgt cgctctgaaa tatgctgtac gagtcaacgg gttacacgcc atagccctca
    33001 ccaagctcga tgtgctgtcc ggtttcgaca agattcccgt atgtacagcc tacgagaccg
    33061 gaggggatga aacaatccac ttcagcggta acacgggttt tctggattcc gtgcgtcctg
    33121 tcttcaagta tctggacgga tggaaaaagg atctcgggaa atccagaaaa atggaagatc
    33181 ttcccgctgc cgcacggaac tatgtcagtt tcatcgagga ggagaccggg gtgcccgtcg
    33241 tgctggtggg tgtcggccca agcagggagc agtcgatagt tcaggggctc tagagaagaa
    33301 aaaatatttg ctgattcatt ataaaacggg gaacggcaag atgccgttcc ccgtcggtct
    33361 tattaacatg gtgggcgaca cagggttcga acctgtgact tcttccgtgt gagggaagcg
    33421 ctcttccgct gagctagtcg cccgtggaac aggggacatt atatccggac agaaggggaa
    33481 aatgcaagag gagccggaca gaatcccggg agaaatgaag atacgaaaaa gcgggatttt
    33541 ttgtgaaaaa gatcattatc tgtccgaatg ccccacagaa gagatatagt gctaaactgg
    33601 attgaagaaa aggttgccac cgcgcgttac tcctttttta aaattgatac agtcaatact
    33661 attgaattat caggaaagta taacgtataa tgtatccgtt tggattggac ggtgaatttt
    33721 tgaaggacat acttaacgac cccaggatgc ttgagatctt ctggagcgag tacggtttcg
    33781 aggaatggtc cggtctggga tacagaggcg tttcccgcag ggtgactttc aggaagagtt
    33841 ccctcatggg ggaggtggca aggtactact ccgacgatta tatcctcagg gcccccgatg
    33901 ccggggctgc cgcaagacat attcttgata actggcaacc ggtgaaggat gtgatgtccc
    33961 accggattct tgtacttgga gatcacggtc tggaaaaaat gttccagagg agttttttct
    34021 ttggtttcaa gggatgggtg gaggtttcgg gatatcgccc cggcgacccc ttgcccaacc
    34081 gcaaatttgc ggatctctgt accctggcca acaatgcagg ggtggtcctt gaccagatac
    34141 ttgagggcat caatcctgta aaggaaagga tcatagatcc cggaaaaagg ggggtgttcg
    34201 cagggaaagg agaaaaggga gatccttcag gttttataaa aaaacttttt cggaggtgaa
    34261 ctaaaatgct tgcaatgctt tttatttttg cgtctgtctt ttttgtagga gcatatttta
    34321 cctacggcaa cttccaggca aagttgtatg gcctcagtaa cgacaacaaa ccgccgtcgg
    34381 aagtctattt tgatggtgtc gactatgtac cagcccatcc ttcggtcctt ctcggtcacc
    34441 acttcgcgtc catagccggg gcgggcccga tcgttggtcc catcacagcg gctgcgctct
    34501 tcggctggct gccgacctac atctggtgcc ttgtgggttc cgtctttttc ggtggtgtcc
    34561 atgacatggg ggctctcgta tcttccatgc gccacaaggg actttcggtg ggtgaagtcg
    34621 tcgacaagtg gataggccga aggggcaaga tgctctttct taccttcaca tggctggctc
    34681 ttgtccttgt ggtggccgtt ttcctccagc tctcggcaaa cacctttgcc gcggacccgg
    34741 cagtggcttt ctccggaaca ctctacatct tccttgcagt tgttttcgga gttcttatct
    34801 acaagaaagg tattcctctc ggggttacca cccttgtgat ggttcccatc gttattgggg
    34861 cggtattcta cgggagttcc gccgaatggg tccagacatc tttcaattac gatgtgaaca
    34921 catggaggct catactcaca gcatacgttt tccttgcctc ggtccttccg gtgtggctgc
    34981 tgcttcagcc ccgtgactat cttgcttctt tcatgctcta cttctcggtc ctgcttggcg
    35041 cgatcgggat gatcttcggc acaaaattct ctacagacct tcccgctttc aagggttttt
    35101 atgctggagg gaccaactac ctctggccaa tgctctttgt catagttgca tgtggggcta
    35161 tctccggttt ccacgccctg gtcggaagcg gcacaacttc caagcagctc aagagcgaga
    35221 aggatgccca gctcgttggc tacggctcca tgctcattga aggcctcgtc gctgttatag
    35281 ccatcggaac ggtaatggtc gccggtggca taatggaggg tggtcctacg gtcacctacg
    35341 gtcatggtat cggtcagttc gccggtctta tcggcatcga tatcaaagtc ggaacatctc
    35401 tcggtctgct ggcgctgaac tccttcctgc tcacttccct tgacacggcc accaggcttg
    35461 caaggtacca gttgcaggaa ttcaccggta tgaagttgaa caaatacgtc gcgacggcga
    35521 taagcgtctt cggcgcgctc ttcctcctct actacaagac cggtggcaag gccgcatggg
    35581 cgctgatctg gccggtattc ggtgcctcca accagctcgt ggccgctctt actctccttg
    35641 ctctggctgt ctgggtcaag aacggtctca agaaggacaa caggtttgcc atgtatccct
    35701 tcttcttcat gctggccacg accatggctg cgctcttctt cctgatcaag acgaacctca
    35761 acaacctgct gctatcggga atctccgttg ttcttgtggt cctggctgta atgctggtca
    35821 tggaagccaa gaaggccctt tcgggcaacg gcccgaagcc ccaggagtaa tcagtataat
    35881 cttctgttac aaaaccagat aaaagcatgg gggcggcaga tgccgccccc atgcttcatt
    35941 ttatcgagcc tcatattata tggaagtaac gttcgatctc ttccctgttc tagccaccaa
    36001 ttttgggaac actgaagctt ttgctccccc atgattttcc cttcacgcgg gagccctcga
    36061 tcagttcgac gaaacgattg taacacattg ggcacttgag ccccggcgcc gtgtcctcca
    36121 ctacgaattc atggccacaa tggagacacc tgtattttct cacttgccaa cacctccccg
    36181 cttcatcgtg aacattgtcg gtttacccgg tgacatcatt ataacttgca ctcagtggaa
    36241 gtggaagcag atttcgctca aaagatggga aaggtagatg tcagggagaa atatactccc
    36301 acaaggagaa gcacaatacc cgagacggtc tggaccattt tctggatcgt atatacggcc
    36361 cccatgcttt ttgatacggc ccgtgtccca agagaaaaaa cgaaggagag gatcgctaca
    36421 gggagagcgc tgccaaggcc aaagagggcg gggagcagga tctttgaacc ggcttccgcc
    36481 gccaggggga cgagaccgcc aaagaagagg gctgccgata cggggcacat ggccatggcg
    36541 aacagaatgc ccataaggaa tgctcctcct gatccccgga acattcccgt caaagtcagg
    36601 cttctggaaa tacccttgcc ggagcggatc tccagaaggt cgagaacgaa aaccccgact
    36661 atcagaagta tgggtccaat aattctgttc atgtgtttct gcatgaatat ggaagcttcc
    36721 ggtatagcca ggatcccgga tattacaagc aggcctgctg tggcgaaagc caccattctc
    36781 cccgccgagt aaagaatgcc cgacaacatt atcctgttcg atgtcgtgac gtctttctgg
    36841 atgaaggaaa aggccgccat gttcgcagca agggggcagg ggctcactga tgccaggatc
    36901 ccaagccaga gcgcagcaag aagtgtcatg ccggtcatcg tgtatccgct ccttccagaa
    36961 acgaatcaga ttcttcgatc aggtatttcc tgaaagaatc ttcgtctcca gagagatccc
    37021 atacggcctc aagattcttc cagcggtata tgagtccatc gctctctttc gccagtacca
    37081 ctgaggttga gaagaggccc aggtcgagga tgtagatttc gttgcctggc tcatccacgt
    37141 ttatctcctt tatcgagaat ctggagatag aagggtttcc ggaactttta aggttcaggg
    37201 tcattgcccg tatcttttcg cacacagggc agggagtgtt gccctcgaag aagtagatgg
    37261 tgtattcaac ctcgcttttg ggcaccgaac cttgaggagc cttgatggat aatgagtccg
    37321 ccctaacggc catgtcccgg tgtatttcgt aagcggccct cgcgaggccc agtagagcga
    37381 acaaaattat tattgttccc aaggcctttt tcgggttcat tttatcctcc tgataaccta
    37441 taatctttac agtcattaca tctttatggt aaatatacca tgaagggtat aaaagctcaa
    37501 tattattcct ggaacctgga ggatccgatg aaaagcgacg atcttgcaca ggaaaaaagg
    37561 atcaaaacgg cggtcaggat caccggaatg acctgcgcgg catgctctaa tgcggttgaa
    37621 agggcactca ggaaggttga aggcgtagat ttcgcggcgg tgaacctttc gaccgagacg
    37681 gccttcatta tccactcccc ggagacaaac cgacttgatc tcgaaaaagc tgtcgagaag
    37741 gttggatatg gggtgaccga tgagatgccg gaggcccttg aaagaaaaag atatctcgac
    37801 tcgagaagga accttgtgga ggctcttgct gtaacagtcc cgctggcagt gctgatgata
    37861 atgcacatgg ctggatatca catggcatgg ttcccgatga tggaacttgt tgccggcggg
    37921 ttcgttgttt ttcgcagcgg gaggaacaac ataaggggcg catggatagc tctgacccat
    37981 ttgcacacca acatggacac cctcatttcc ataggttcaa tagcagcctg gctgacttcc
    38041 ctgttgcacg ttacaggagt tcccgtggcc tcttttggca gcataggagc catgatagtg
    38101 gctctccaca tcctgggtcg ctttatcgaa tcctccctca gggacagggc ggcgaaggag
    38161 atcaagaacc tgctgaaact gagggccagg gaggccaggg tggatgtcaa tggcaaagaa
    38221 atgaccatcc cgatagaggc tgtaaaaagc ggaatgaccg taagggtcag cccgggaggt
    38281 ctcatccctg tggacgggga tgtgatttcc ggaagatcct ccgttgatga atccatgata
    38341 acaggggagt ctgttcccgt aatgaaagaa aagggggcgt ccgtaacagg gggttccatg
    38401 aaccttacgg gggaaatgtt cgtatctgta acaaggacgg gagaagacac attcctttcg
    38461 aagatggtat cgctggttca ggaggcccag ggttcgagga tacccattca ggctcttgcc
    38521 gatgccgtaa ccagagtttt cgtccccgta gtcacaattc ttgcccttat atcgggtttt
    38581 acctggttcg tgtttttcag ggaactctat ccgggactcg aaaccctgag acactggttc
    38641 ccatggatcc tcgagacaga tagtccggcc tctttcgggg ttttcgtctt tgtggcaacc
    38701 atggtgatcg catgcccctg tgccctgggt ctggctacgc ccatggcact tgtcgccggg
    38761 accggaatgg ctaccaggcg ggggctcctg ataaggaacg ctgaagcgat ccagacaacc
    38821 agggatatag ggacagtcat catggacaag acgggaactc tgacggaggg ggatccgaaa
    38881 gtcgtcttcc atgacctttc cagggaagtt cttgcggctg tggctgccat agagagacgc
    38941 tcggatcatc ctcttgccag ggcgataggc ggtgcttttg aagggaccct gcccgttcca
    39001 tcggatattg tagagattgc cgggaagggt gtccggggcc ttgtcgaggg atctgtgtat
    39061 gagataggta aaccgccctt gcccgaagcc tacatggaat atcttgaaag aggtgcgacg
    39121 gtggttgagg ttaacagaga tggaaagccc tcgggttttc tggtcataga ggatcccctg
    39181 agagaggatt ctgtaaaggc cgtggaagaa atgaagcgaa tggggatcac accggtaatg
    39241 ctcaccgggg acaatgaggt caccgcaagg tcagttgcgg tgaagactgg tatttccgaa
    39301 atatatgccg gtgtcatgcc ggaaggaaaa ctggagatag tgagacgata ccagtcggag
    39361 ggcgcgaagg ttttgatgat aggggacggg ataaatgacg cggcggcgct gaagggagcc
    39421 gatatcggta tggccatagg ttcagggatg gatctggcaa tagacagtgc cgacgttgta
    39481 gtaatgagtg gagggatatc gagggcagtg gagtgcatca ggatatcggg taagaccttc
    39541 agggtcatca ggcagaacct tttctgggca tttctgtaca acctggtcgc tatacccgtg
    39601 gcaatgggag ggctgctcca tccggccata gccgaagggg cgatggccat gagttcgata
    39661 tcggttgttc tcaactcgct gaggatcagg aaggaggagc attgatggga gaaaggctgg
    39721 tctatggagt tcccgccatg tcctgcggac attgtgtgag gaggatatcg gaggttctgg
    39781 tttcacttgg agtcgagggg tttgatgtct ccctcgaaaa caaggttatt tatacggatg
    39841 accctctcga cgaaaaagtc ataaaggctc tttctgatgc cgggtacgag gtatttgtga
    39901 gataggacaa gtgctgctgc cctgctgctt aaggggctga aatttccttg taaacattgc
    39961 tgatccgtgt tagtctcagg cagtgcttta aatcagttaa cagcgatagg gctatctctg
    40021 tgtattgtca gaaggagggt tgtttgatgc cgcagttccc gcatgaaggc tttagcgatc
    40081 tttccattgt gttgtgtggt gccgcggggc agggggttca gactgtcgag gagattcttg
    40141 tggattctct aagaaggtct ggcctgaatc tgttcgcgag cagggaatat atgtccaggg
    40201 taagaggtgg aagcaactct acggagttgc gggtctcgtc ggaaagggtg aacgcccttg
    40261 tggacaggat agatattctg cttccgctca acgggggtat caggcccaac atacggaaga
    40321 gaatatcgcc cgggaccctt attctcggtg atattgagga actgaaggac gaattcgggg
    40381 attctcccgg tattttcatg aatgtccctc ttatggagaa agggagggaa tccggcgggg
    40441 cggtttacac cggagtggtg gccgtaggcc ttctgtcagg tctgctggat ctgccgacgg
    40501 gaataataag atccattatt gaggagaaat tctctggaaa atctgccgag atagtgagaa
    40561 ataatctttc cgcccttgaa aaaggacttg agatcgcttc agaactaaga tcttcggggg
    40621 atatcgtggt ggagatcccc aatccctccg ctggcgctaa ggagatggtg ctcaacggat
    40681 ccgatgcggt ttccctcggg gctattgccg gagggtgcga ttttatcgct tcctacccca
    40741 tgtcccccgc tacgggtgtt ctgacattta tggccaggca tgccagaaaa tttggcgttg
    40801 ccgtggagca ggccgaggac gagatcgcgg cgataaacat ggccacaggt gcttcatacg
    40861 ggggagccag gtcaatggtg agtacttccg gcggtgggct ttccctgatg tcagagggag
    40921 taagcctggc gggaataacc gagatcccgg tggtgctcca tataggtcag cgtcccggcc
    40981 cggctacggg tatggccaca aggaccgaac agggagacct taaccttgcc gttttttgcg
    41041 gccacgggga tatccccaga atagtttttg cgccggggac cctggagcag gcattcagat
    41101 acacgagaga agctttcaac ctggcggcag agttccagat ccctgtattt gttctgacag
    41161 accagtattt cctgaactct ttctacaaca tccctccatt tgaccttcaa gacttcagag
    41221 ttgaaaggca tcttgtcgag gccgtcccgg gctacagaag atatgaaaac actcctgatg
    41281 gagtctcccc gaggagcgtt cctggttttg gaatggaact ggtcgggaca gacagccacg
    41341 aacacgatgc atggggtcat gctcaggagg attttgaact gagaaaaagt atggtggaga
    41401 aacggcttcg gaaaatgaag ggtatcttgg gaaaagccga gaaaccgacc ctggaggggc
    41461 ctgaagattt cagggatctt gtggtttcct ggggatccac ctgtcacata gttagggaag
    41521 cccttgaaaa gacaaatagt ggaaaggtcg ctcatctcca ttttggacag gttttccctc
    41581 tccacagaga tacggtggac attctggcga gtgccgaaag ggtcgtggct gtggagggta
    41641 acgcgacggg acaatttgcc gggctcatca gaaaggaaac agggtttttg atcgaagaaa
    41701 aggtgctctg gtacagcggg ctccagatgt ctgtcgagac ggtagctcaa ggcctgaaag
    41761 aacgcttggg acagggaggt tagagatgga tcacagaata tatgatctgg aagggatcga
    41821 caaccagtgg tgtcctggat gcggagactt tcagataatg gaggctttga aaaaagccct
    41881 ttccgagctt gatattcccc ccgagaaggt ggtcctgttc tctggcatag gacaggcggc
    41941 caagatgcct cattttatga gagggcactt ctttaatggc ctgcatggac ggtctatctc
    42001 caacgcgact ggaatgaagg ccgcgaaccg tgaccttacg gtccttgcga tcggagggga
    42061 cggggatatg tatggcgagg gtggaaacca ctttacccat acgatccgtc gaaatccgga
    42121 tatcacgaac ctcgtccaca acaacagggt ttatggtctg accaagggac aggcttcccc
    42181 cacaagcccc aggggttttg taactcctgt acaggtggac ggagtttttc cggagccctt
    42241 caatcccgtc gcgatcgctg ttgcccaggg cgcgtccttt gtggccaggg ctttttcggg
    42301 gaatgtggag cagacagtga gtattatcaa acaggcgata cttcacagag ggtattctct
    42361 ggtggatatt ttccagccct gcgtatcctt caaccgcgta aacacctttg cctggtacag
    42421 ggagaacact tacgagatcc cggcgggaca cgatccgggt gacaggaagg cagccctcga
    42481 cagggccatg gaggaggcgc cctatcctct cggggtaatt ttccgggacg gggaccgaaa
    42541 gactttcgaa gattctctgg cggtgtacga ggaggacgac aggccgcttt tcatgaggga
    42601 atacggcccc gaaaggataa aagattatat ggcctcattg atctgacatg atcggatcgg
    42661 aaggcgaaaa aacagcaagg gaggaaagga taatggagtt taaaaagacc gcagcggcaa
    42721 tagggatcct tctgttgctc gcgacgacag ctgtcggaca ccccccttca tctatggaga
    42781 tcgaatattc gaaggatgac ggagttcttt cgataggtat cgttcaccct gtcggcgatg
    42841 tgtcgacaca ctttatcgag agcgtaaccg tttcggtcga tggcaacacc gtggcggatc
    42901 tggtctatct ggagcaatgg gataaaggcg gggagaagat agttgttacg atcggcaggt
    42961 tcggcccggg gtcgaagata tccgtaaaag cggtatgcaa caagatcggt accctcgaaa
    43021 aaacattgga tctgtaggtt tatcggggga aaaaactggc ggggcaaaaa gccccgcctc
    43081 aaattatttg gcgtcgctca cttccagtcg aggttgagaa ttctcttcca tgtgaagttc
    43141 tggatgacct ccgttgaggg ctttcctccc cttgacagcg attcgtaatc cgaaagcagg
    43201 agggaatagg cctccttgcg ttcggggatc tgctccgcgt cccttaccat tgcggtgatc
    43261 ttgtgtaatt gtctatagac ctccgggatc acctggaagt tggggccttc acctgtgaga
    43321 gctattgtgt agacaaaagt cagagtcctg cttcccccga actgtggaag atccttgtat
    43381 gcggggaggt tcggagagac gaaaagccct atcccccttg gcgcgtatac cttgacgggt
    43441 atggaatccc aataggtctg tcccagaggg ctgattatta cccaggctct cggggtgttt
    43501 gtggtcacgc cgatccagag gtatattcct ctggatcgat ctccgggtct tgtcagtgtt
    43561 atggaccaca aaagatcttt tctaaagtca ccatcggcgt atggttcgac gacgttttcg
    43621 cctgaataga cgtatccctc gatcggaagg atcttctgta tacctgcggg tgatcttaaa
    43681 agggccgatg ttatgactcc ctcgcttacc tccagaacca ggttgacagg atggcccggt
    43741 accgagagta tcttgagata ttgcgcatgg gaagaagccg ggaaaaagac cgcgatcgca
    43801 aacatgacgg atagaaggga cgcggttttt ttcattcgag agatcaatgc tatcactcct
    43861 taagagatgg gtatatcaaa gactggttat cttctcaata tttttatgtt cgacctgttc
    43921 ggccagttcc ttcacgcttt tgccgagaag ggggtggacc cagtccggtg ctatctgcat
    43981 aagggggacc aggacaaagg ccctccggtg catctcctgg tgaggaataa cgaggtcctt
    44041 ttcgtttacc gcgatgtcgt ccatgaacag aatgtccaga tctatctccc ggggtcccca
    44101 ccggaacctg tcgatcctcc ccatttcgtc ttctatggat ttgagttttt ccaacatttc
    44161 cccgggttca tcatcggact cgacaaggat gcacgcgttg agaaaagccg gctggtcgac
    44221 cactccccat gggggtgttt cgtagacatc gctcaaggcc agtatcctga gccctttttc
    44281 ccgaaggagg ttcaaggcct ggcgcaaaca ggaaagcctg tctccgaggt tgctgccgag
    44341 agataatccc gctcgcatca ggcctttccc cctttcagtg cctctatcat ttttacggct
    44401 cgcagattct gtcccacatc atgcaccctc acaaggggga cttccttcat ggcgcagaag
    44461 gccgaaatgg caagggttcc ctcaaggcgc tcggaaggtt tgtccagttc cagtaccctt
    44521 ccgatagaac tcttccttga gtgtccgacg agaatcggtc ttccgagggt ggaaaaggaa
    44581 tcgatctgtg caattacctc aaggctctgg ctggaattct tggaaaaacc gatccccggg
    44641 tccaggatca cgctctgtgg cgagatcccc gcttctgcgg cggaccttat acggtcgaag
    44701 aaaaaagccg ttatttcccc gatcagatcc ctgtaggcat attttttgtg catcccgtca
    44761 gggacttcca tggcatgcat aataacgaca ggattcccgg tcctggccag gagagggaac
    44821 atcagtgggt cgaaaatgcc accggaaatg tcgttaataa tgtctgctcc cgcctcgata
    44881 gcctcggaag ccgtgtctgc ccggtaagtg tcggcggaga tcaacgcctc cggaaccctt
    44941 tcccgcacag cttttattgc cggtatcagc ctgtctcgtt cttcgggacc cgatagaggt
    45001 gtggatccgg gtcttgtcga ttcggccccc aggtctataa tgtccgctcc ttccatgtcc
    45061 attttctcgg ccatgtccgc gcatgcctcc gtggtggatg ccctgcttcc gctgtagaag
    45121 gagctgtcgt tgacgttaat tattcccatt atggccgttc tgccgcccag atcaagggga
    45181 ggtcgtcccg gaagcgggat ctcccacttc ctgatattgg cagctccggt ggcggcttca
    45241 agttcttctc tgacgacatc caggccccag tagggcatgg ctccaagttt ttcgagaagg
    45301 ctactgaaga ctttgcgggt tcccatcaat attacatcgc acctggctac agatccgtcg
    45361 atcgcccccc ggtggactat ggcgtcaccg tctctggaaa gcatttcctg cttaagggcg
    45421 ttggcggcac ggaaatcgat atctgcaagg tgaaaggcca gtatctctct tttctgatcg
    45481 aaatagggca tactgcggac atcggcccct gtaagggaga gggccttgcg caggtcatgg
    45541 tcgttctgga gatggaagcg gtatacggtc attattttcc tgctctcctg tttgaaggat
    45601 gagatctggc cgctggggct cagacccact cgctgataat gtgttcgaga tgagatgtct
    45661 ggttgagcga aacaagacag taccctcttt ctttttcgaa ggtcattacc gagaaggaag
    45721 cggtatcgat atggatcctc cagaaatatg gatccctgat gttgattgca gctgccagaa
    45781 ggggcttcag gacagccctg tgggaaacga ttgtgaaggt ttctccctcg tggatgttta
    45841 ccaggttgtc caggtttgcg aaagcacggc gctggacatc cgagagggtc tctgcacccg
    45901 aaatgtgcag tctctccggg tgagaaagcc acagcctcca ctcctcgggg tattcctgct
    45961 caatttcttt cttttttcgg ttttcccatt tgccgagggc catgttgtta aatccctgac
    46021 ggatctcaac cggtaccccg ccttcttcgc cgattgcgcg ggccgtctcg atgctcctcg
    46081 caagggggct cgaatacact acggaaatat cctgtgacga aagaaccttt gccagagctc
    46141 tcgcctgttc caaccccgat ccgttcagag ggaaatcggt tctacccctg aaaagaccct
    46201 ccctgttacc ttcgcactct ccgtgtctta caagtactat tgtggtcctt ctttttccgt
    46261 tggacaaaac gaggtccctc caaagtagca gaatactgaa aaccggggac tattttaggc
    46321 taaagagggg acgatttggg gaggttttca tctgaccagt gttgacaata ggcgatgcga
    46381 ttgctaatct acctttcggg tccaaagctg ttagcactcc agtattgaga gtgctaatga
    46441 catccaggga gatgtttacg gtgctgagtg aaagacatct tgaggttgtt cttgctgttg
    46501 tgtacgaata tatcaagaca ggagaaccca taggatcccg gaccatatcc aggaagtacc
    46561 tgagggaccg aagcgcggcc actatcagga atgaaatggc agatctggaa gagatggaat
    46621 atctctacca gccacatact tccgcgggtc gaataccgac acccagggcc tacagggttt
    46681 atgttgacgc tatcctcaga cgacagcgct cctttcccct ggaagatgac ctttgggtcc
    46741 gggaactgag ggcccgaaga aaggatgccg aggaggcttt ggtctatgcc accaggctcc
    46801 ttggaaagat aaccagttat atcggtgtgg ctgcccttgg ccctgtaagc gaaacagtgc
    46861 ttcagagggt cgaattcgtt cggatggggc accggacctt gctgatcctt cttgtgctca
    46921 agggtggtct gatccaccac aagaccgtaa ctctgccatg tgatatgaca caggaagccc
    46981 ttgacgaact ctccagaagg gtttcggtag ttgccgtagg tcgttcctgg aaggacgtaa
    47041 ggggtgttct ccagtcctat gttgaggatg ccctcgagaa gtacgccgag aactgcaggt
    47101 ctgccatttc tgaaatggac ggaataatga tggaccagac cttccagttt tttactggag
    47161 ggacccacaa tgtccttggt atgcccgatt tcagcgatct gggtcgcctc caggcaataa
    47221 tggccctcct ggaggagggc gagtctatga gcaaactggt caacgactgc tcggccgagc
    47281 agggattgtg cataaccata ggtgatgaaa atcccgtgtt tcagatgcgg gactgctcaa
    47341 tagtaatggc ctcggccaaa accgggggaa gaaaggctgt cgtggggctg atcggccccg
    47401 tgcgcatgga ttacgagaga tccatatcgg ttctggaggg aatactggat actctttccg
    47461 cggacgtaga agtcgaatag ggaggcattt caatgaaaat ggcagaagat ggcaagtttt
    47521 ccgaaaaaga acaggaggag gccctggggc ctacgggcga tgaaatagag gaagttgtcg
    47581 ggcacgacct tcaggatggg gaagagaatg tagatgagct cagagcctcc agggagttgg
    47641 cagagaagct catggaagag aacgaaaagc tcaaggatgc cgctgccagg acgagggcgg
    47701 atttcttcaa ttaccgccag cgggtggaac gggacaggga acgtctcgcc agatctgcct
    47761 ctgaagatgc cgccgaagcg ctgataccgg ttcttgacaa ccttgagaga gctctttcgt
    47821 ctccatcggg ggatttcgac tctcttttca aaggcgtgga gatggtgagg aaccagttct
    47881 tttcggttct ggaaggcctg ggcctttcca ggatagagac ggggggacaa ccttttgacc
    47941 ctgcctttca tgaggctgta gcgatagagg agatcgaaga cgaatccctc gatggggtta
    48001 taacagagga attacagccc ggttatacca tcgccgggaa agttttaagg gctgcaaggg
    48061 taagggttgc cagagcaatt cagcgtggta cagaacaata aacgacatac ggggtgatgt
    48121 ttgaatgagt aaggttatcg ggatcgatct tggaacaacg aacagctgtg tcgctgtaaa
    48181 ggaaggcgac catgttacgg tgataactaa cgccgaaggt gcaaggacaa ccccttcggt
    48241 agttgctttc actaaagaga aggagagact tgtcggtcaa ctggccaaac gtcaggccat
    48301 agtaaaccct gagaggacca tatcatcgat caagcgccac atgggttccg atcacaaggt
    48361 tgaagttgac aatacagccc ataacccgca gcagatatcg gcgatgatcc tccagaaact
    48421 caaaagggac gcggaggaat acctcggcga aaaggtcggg caggcggtca ttactgttcc
    48481 ggcctacttt accgatgccc agaggcaggc caccaaggac gcagggcgca ttgccggtct
    48541 ggaggttctt cgcataatca acgagcctac tgctgcagcc ctggcctacg gtactgataa
    48601 aggcggggat aacaagatcc ttgtttttga ccttggaggc ggaactttcg atgtttctgt
    48661 tctggatgtc gggatcgagg acggggttat cgaggttctc gcgacctccg gcgataatct
    48721 gcttggaggt gatgactggg atcagaagat catggatctc atggtttcgg agttcaaaaa
    48781 gagtgagggg atagaccttt cgaaggacaa gatggccctg cagaggcttc gcgaagctgc
    48841 cgagaaggcc aaggtagaac tttcgacaat gtcggagaca tccatatccc ttcctttcat
    48901 cactgccgat gccagtggac caaagcattt cgagatgacc cttacgcggg cccgtttcga
    48961 agagataacc aggtcccttc tggacagggt tgttcagccg accaaaaggg cgctgaacga
    49021 ttcgggcctg acccgcgagg agataagcaa ggtccttctt gtcgggggtg ccaccagaat
    49081 gcccatggtc cagaaaaaga tagtggaact actcggcaag gaacctacga aggggatcaa
    49141 ccccgatgag tgtgtcgccg ttggcgccgc gatccaggga gccatactta caggtgagca
    49201 caaggatatt atcctggtgg atgtcacgcc cctttcgctc gggatcgaga ccctgggagg
    49261 cgtttttaca aagataattg acagaaacac agccataccg gtctcaaaga gccaggtatt
    49321 ctcgacggcc tccgacaatc agccccaggt agagatccac gttcttcagg gagaacgatc
    49381 catggcggga gacaatgtaa ccctgggcaa gttcttcctt gacggtatcc cgcctgcacc
    49441 gagggggatc ccccagatag aggttacctt tgacatcgat gtaaacggca ttctcaatgt
    49501 cagcgcaaag gacaagggaa catcgaggga acagcatgtc acgatccagt cctcgagact
    49561 tgccgaggac gagatcgaga agatgacgag agaagccgag gagaaccagg agtcggacaa
    49621 gaagaaaaag gaacttgtcg atgcgcgcaa cgaggcggac tctcttatct acagcacgga
    49681 gaagatgatc tccgatctag gggaaaaagt gaccgaagaa gagaaaacca ggatcagcgg
    49741 agagatagaa gatgtcaaaa aggccctcgg ggaagagaac ccggagggta tccgccaggt
    49801 ggcggagaaa ttgacgaaga gccttcatga gatgtcttca cggctttacg cccaggccgg
    49861 ctctgaggat gcctccgatg gtaccgaacc agaaggcagc gacggcgaag agaccgtcga
    49921 cgcagagttc gaagacaacg gaaaggtctg aggagtgaaa cggtttgccc gcaggagatg
    49981 aaagggatct ttacgcgatc ctgggtgtat caaggaacgc gacacaggag gaactcaaga
    50041 aggcttacag gaaacttgtc cgaaagcatc atcctgatgc caacccgggt gatcccgagg
    50101 cggaatcgaa gttcaaatca ataaaccttg cttatgagat ccttggagat ccccagaaaa
    50161 gggccgccta cgatcagtat gggaccatag gggatacgcc gggaggatcc ccttttggtg
    50221 gaggattcga gccctttggg gatattttcg gggatctttt cgggagtttt tttggaggcg
    50281 gacgaagagg accagctggc ccttcaaggg gcgatgatct tgaaatgagt ataaaaatat
    50341 ccctcgagga cgctttcaga ggcctgggca gggaagttgt cataccccga atggatacct
    50401 gcggacgttg cggaggatcc ggggcggagc cgggtacctc cgtaaaaacc tgttcccatt
    50461 gtggaggttc cgggcaggtg gaaaaacagc agagaactcc ctttggtacc ttcgtcagcg
    50521 ttggacagtg cccgcattgc ggaggttctg gcaagaggat agaaagcaga tgcaagaact
    50581 gtgggggaag cggtaaggta aggtccgatc accgcgtcga agtgaagatc cctcccggag
    50641 ttgatacggg aaccaggctc cgcatcgccg gggaggggag ggagggctcc tccggcggcc
    50701 caccaggaga tcttttcata tcgatcaagg ttgagccgca cccgcttttt gaacgcgacc
    50761 gggacgatct ccatacaaga gtcaacctga agtaccccca gatggtactg ggatgctctg
    50821 tggaaattcc gggtatcgag ggtggagaaa aactcgaagt ttcccctgga acccccgtgg
    50881 ggaaggctgt caggataaag aacaaaggca tgccgaggct gagaggttct gggagagggg
    50941 atcttttcgc ccatgtttac gttgacattc caaccgaact atccgaaaag gagcgccttc
    51001 tgaccgaagc cctggcacag gagatggatg ttggagtaga gcctgcgggc tttatggaaa
    51061 aaatccggaa tcttttcggc tgatgccgca aatgcttttt ccgtggtaga ctttccatta
    51121 gacgggaggc gtcatagcct cccgtatttc atctgcaagt caaacttgcc gggggtaggc
    51181 gtctatgtcg tccaatgaag gtttctggtg gtacataact attgaagggg attcagagag
    51241 cgaggaagat cttgtatccc ttggcgaact ttcgggaagt ataggctcgg agatgcagga
    51301 attgccgggt aaggtcgccc tcaaggccta ttacaggaac accagggatc tgggatcctg
    51361 gatggagaga gtgcaggaag taatatcccc gtggcccggg gtccggatag ccgacatggg
    51421 gcgtatagag aacaggcagt ggcatacggc atggaaagag gcctttcccc ctcttcctgt
    51481 aggggataat ttcattgtga tggctccgtg gcaccgggga aatgaacccg aaggaaagat
    51541 ccctgtatat atatatcccg gaagcgcttt tggtaccgga taccacgaaa gcacgcagat
    51601 cgcgctggaa ctgctggaaa agaccgtaaa gcccggtctt tccgtagcag atatcggaac
    51661 cggatcaggg atcctgtcca tcgcttccgc ccttctgggg gcgggccagg ttctggccag
    51721 ggacaacgac ccggccataa tcaacgaagt aagggagaat cttcgactga acgatatttc
    51781 gcccgaaaag gtccttgtac agacgggaga tctgcttgcc ggtgtctcgg gcaagtacgg
    51841 tattatctgt gccaacattg tttttgaccc cctggcttcc atgcttccat ctgtcccttc
    51901 cctgctggaa gagggtggag tggcggtctt ttcgggtctt acgcttaagg aaagggatcc
    51961 tttcctggtg gaggtttcct cagccgggtt gtcaccggtt accgagttga caaagggaga
    52021 ctggtggggt gtcgctgcct cgcgtacgcc ttgagcattc tgttcaggaa agtgattccg
    52081 tctgggtcct ggacggggaa cagggaaggc atctgctgac agtaaggcgc tgcagggatg
    52141 gcgagttttt cgagggtctt ttgccgggca gaagaatact gttgcgcctg gagacatgtg
    52201 ggacattggc cagaggtgtc aaggtcaggg aaacgcccga ggaaccgatg ccggaaatct
    52261 ggcttctggc gggtctgctc aagggagatt ccttcgatcg tcttctttcc cagtctgtcg
    52321 aggtcggtgc ttcggttatc gttccccttg tctgcacacg atcggtaccg atcctggaac
    52381 cttcaaagat agcccgaaaa atggaacgct ggaaaaaaat aaccctcgag gcctcgaagc
    52441 aatgctgcgc gccgacaccg acgcggatcc tggatcctgt cggtatatct tccctcggtt
    52501 ctctccctgt tccatccgcg agattcgtcg ccgttgcggg ggcaagttca aaaatttcgg
    52561 aattcagccc gccgggaact gccgccgtcg ccaccggacc tgagggagac tggacagaag
    52621 aggagataca tattctgcag caacatgact ttatcccggt gaacctgggt cttcgggtca
    52681 tgagggctca cacggccgct gtggttgcag tcgcttttct tgccttgctc tctggaggtc
    52741 agaagttgtg aaggatccgc taaggggagt taaattcagg gttattccca tgggctgcag
    52801 gacgaacctt tttgagtgcg aggcgctttg ctgttccttc gttgagtctg gtgcggcaag
    52861 atccgaaaca aaagattttg acgttgccgt tctcgtagga tgttccgtta cctccgaggc
    52921 cgacagaaaa tgcaggcaga tattgaggcg tctcagaaga gagaaaaaag acgcgctcat
    52981 cattgctgct gggtgctggg cacagaaagc aacgatcgag gaggcggccg gtctgggtgt
    53041 tgactgcctt gtaggaaaca ggctcaagca caggatcccc gccattgtca gggagatgct
    53101 tctctccggc agaagaacgg aacagcccag gatcgagatg gtggatgttt caaatttctc
    53161 cgggtgggag gatcttcgcc ttctgaggcc acagaccagg acacgggctt tcgtcaagat
    53221 acaggatggt tgtgatcatg gctgttccta ctgcataatt cccgctctca gggggaaagg
    53281 ggtcgaaaga gatccccttg atgtactcca ggaagtggaa aggatagccc tgtcggggtg
    53341 cagggaagta gtttttaccg ggataaatct gggagtctac ggcaggaact ccggaatgcc
    53401 cctgggagaa cttgtcaaaa gaagtgggga tatcgaaggc atagccagga tacgttttgg
    53461 ttctctggag ccctttgccg tcaccgaaac tcttctcggg gatctttccg caacggcaca
    53521 gttctgtccc cactttcacc ttcctctgca gagcggtgac ccggatatac tcaggcttat
    53581 gcgcagaggc tacacgcccg aagatttcct ggcccatgtt gaaatggccc gctccttttt
    53641 cggtgaggac cttcacatca gtacggatat aattgtcggg ttcccgggcg aaagtgaaga
    53701 tgcctttgac aggaccctgt ccctgctcgt gtctgccggg acagggaggc ttcacgtgtt
    53761 tcctttctct tccagggatg gcaccgtcgc tgcttctata ccgggtaggc tgaagggtcc
    53821 ggaaatcagg tccagggtgg aaaggtccat agccctggga aaagatcttc tcgattcata
    53881 ttctggaaaa tggatcggga aaagggtaga tctgcttgtg gaaggaagaa ccgggggcat
    53941 gatggagggt ttttcctccc actatgtccg tgtggaatgc cccggagact ctgaaccggg
    54001 aatgatcagg agcgtttttg tcacggattc tctggatggg gttctcaggg ccgtcgtgtg
    54061 atataattcc ctaccaggaa ttgcggattg cgttcataca ggaaaccggc cgatatattc
    54121 agggcgaggt gcggtcatgg ggaacgggag cgaaaggtgt attttttgcc gtatcgccag
    54181 gggccttgag aaggcggatg tcgtatacag cgacgaagat gttatagtta tacgtgacat
    54241 tcacccgata gccccggtgc accttcttgt cataccgagg attcatgtcg aatccgccga
    54301 caaggtggcc gatccatcgg tctggtcttc ggtgatgggg gcggcggtga gagtggcccg
    54361 tgatatgggt ctgggccctg acgggtatcg tctcgtggtc aactgtggag ctggggccgg
    54421 gcaaacggtg tctcatcttc atgtgcatct gcttgccgga cgctctttcg gttggcctcc
    54481 aggttaaccc tgagtgagaa gagaaggggg ggaacgcaat tgacacaggt cgttcgacgc
    54541 gataacgagt ccctcgaaga tgccctcaag cgcttcaagc gcgaagtatc caaaggaggc
    54601 gtgttgcgcg aggcccgcag acgttcgcat tacgagaaac cgagcgaggc aaagaagagg
    54661 aagagggaag atgcttccag aaagcgcagg aagtaggtaa ggttcgatga ccgaactgga
    54721 ggcgaggata catcaggaga tagtttccgc catgaagagg cgggaccagt cctctctcgt
    54781 gcctttaagg atgctcaagg cggccgtcga aaatgcaagg atagaaaaag gccgtggaga
    54841 gcctttttct gaggaagatg tcttcgccgt aataagaaaa ctggtaaaac agcgtaagga
    54901 atctgccggt cagttcgagg ccggaggagc tcttgaaagg gccggagagg aactgatgga
    54961 agcggaattg ctctcatctt tcctgcctcc ctccatgagc gacgaagata ttatccatga
    55021 agcaggaaag accatagccg cagttggcgc aaaaggcccg tcggatctgg ggcttgtcat
    55081 gggtccctgt atggctgccc tgaagggcag ggcggaaggc gccagggtga gaaagatcgt
    55141 agagaatatt cttcgctctc tttaggcaaa tgacatcgcc tcttttttca gaccccgttt
    55201 acagcggggt cttttttttg tcttgaacag gggtacatac acaaaatata gtggtataat
    55261 ccaggcaatg aactaaatgt ggggagtggg gccatgcgtt gtccaaaatg cggatacatg
    55321 gagacccgtg ttctcgagac gaggacggca agcgaaggca aggtcataag gaggcgtcgt
    55381 gagtgtcccg agtgcttcaa caggttcacc acttacgagc gtctggatga aaggcccgtt
    55441 ctctggataa tcaagaaggg tggccagagg gagggttttg acagggcaaa gcttctcaag
    55501 gggctagcca aggcctgcga aaagctgcca gttccgctgg agcagatcga ggaagcggcc
    55561 tccaggatag aagagacgct gcgtgagatg ggtcagggtg aaatttccag tctccaggtc
    55621 ggatcactgg ctatgaatga actgagaaac attcacaagg tcgcctacgt gaggtttgct
    55681 tctgtataca gagagtttac ggatatctcc agttttgtcg aagaaatttc ccgacttcac
    55741 gacgaaagga atggtcattc atgaaagact ccgactcccg gatcatgatg gaagaaaagt
    55801 tgtcccccgg ggattttcca ggcggaagac agatgcttat tttttcgggg aaagaagaat
    55861 ccaccgatct ggctctcatg gtggaggctt ttggacagga ggaccgttca gcctgggatg
    55921 tcgtgcggat ccgtgatgcc cttgtagtgg aggcgggagt tgatccttcg gtggctggag
    55981 agatcgccgc agaggttgag agcgatatta tgatacatgg aagggaaagg gtcaccaccg
    56041 cgatcatcag ggagatggtc aacgtaaagc tctttttccg gggtctcgat gccaaacttg
    56101 ccgatcattc caggctgggt ctcccggtgt tcgacctcga gtcgatgatg ctgcatccca
    56161 acagggagaa cagtaatacc tcccacaacc ccgaatccat aaatctctcg gtagccgaga
    56221 tgatactcaa ggaatatgcc ctagggaaga ttttttcgcg tgaggtggcc gaggcgcatc
    56281 tcgggggaga catccacctt catgacctcg ggatggtaaa ccgcccctac tgttccgggc
    56341 agagtatggc ttacgtcata aaatacggta tcgatatccc ttcgataacg agtgtttcct
    56401 cgcccgcccg ccacccggac gttcttcttg cccatattct caagatgacc tcggtacttc
    56461 agaacaactt cgccggggca ataggctggg acgctgtgaa tgtctttttt gcccctttcc
    56521 ttgaaggtct tccatacgag aaagtgaagc agctcgccca gatgctcata ttcgagttca
    56581 accagctggc gggaggcagg ggaggacagg tggcgtttac ggacatcaat ctctactacg
    56641 agatccccga ccatttcagg gatgtgccag ccctgggtcc cgggggagtt ccgacgggca
    56701 ggacctacta cgattacagg aaggaatcaa aagatttcct ccgcgccctt ttcgaggtct
    56761 acctcgaggg ggacagccgg ggacagccct tcttttttcc caaaccgctc cttcacatca
    56821 ccgacaggtt tttcgaggag cctggctggg agaagtttct tgaacttgcc tgtcttgtcg
    56881 cgtcggaaaa ggggagtact tatttcgtat tcgacagggg cggggttgcc aagttgagcg
    56941 agtgctgcag gctgagtttc gaactttccg aggaggacct ggcggaagcg aaaacgccgt
    57001 ggaagatgcg ctactgtgct ctccagaacg ttaccataaa tctcccgagg atagcctaca
    57061 ggtcgggcag agacagggag accctttttg aactcctcga cagggccatg gagacggcgg
    57121 cggaggcaca tgtccagaag agaagatttc ttcaggagat actggcactg ggttcaaagg
    57181 ggcccctgtc cgttctttgt gtcgaccacg atggagagtc atacctcaga atgagcaaga
    57241 gcagtcacct cataggcata ctggggcttg atgaaatgat ccactccttt acgggtacga
    57301 ggatgcacat gagcgacgag gccatggacc tgggtcttgc tgtgaccaga tatatggatc
    57361 taaaatgccg ccaactttcc gataggcttg gactgaaaat agttcttgag cagacccctg
    57421 ccgaaagtac cgcctacagg ctggccaggc tcgacctgaa atttttcccc gatgatgcgc
    57481 gccattttgt caagggagat atttcaagtg gtgagatcta ttacaccaac agcactcact
    57541 ttgcctatga cgccgatctt gatccggtat cccgggtgat aatggagggc cgttttcacc
    57601 ctatgatcaa tgcgggagcg atatctcatg tctggatggg agaacacagg ccagacccgg
    57661 gagcgctcgc gtcttttgtg agaaaggcct acgaaagttc ggagaatgcc cagatcgctt
    57721 tcagcccgga gtttacgata tgcaacaaat gcggaaagat ggaaagggga ctgctggatt
    57781 catgtcccgc gtgcggaagc atggatgtgg acgggattac ccggatcacc ggatatttta
    57841 cgaggacgtc gtcctggaac gcgggcaaaa gggccgaact gagggatcgt tcaaggacgg
    57901 cagtctgaaa aatgaagcct ggagttccca tcggagggtt tttgccggta tcatttgtcg
    57961 actgggccgg acatgtcgct tcggtccttt tcctttgcgg gtgcaatttc agatgcccct
    58021 actgccataa cgcggatctt gtttttgcga cgaaaggatc catcgatgcc gatgatgttg
    58081 tcaaaaaaat cctttcaaga aaagattttc tcgatggcat cgtgatcagt ggaggggagc
    58141 caacaatcca ctcagggctt ccccttttcc tttctttcct caaaggggaa actggcctga
    58201 agatcaaggt tgatactaac gggagcaatc ccggaatgct tgagacgatc atagccgaag
    58261 gtctggtcga aggtctttcg atggacatca aggccccgtg gaatgactac ccacgaataa
    58321 cgggcagcgg ttccgatgag gtggcaaagt ccctggatct tgtccgccga tcgggtattc
    58381 cccatgagac caggaccacc ttcgttccgg acatcatgtc aattgaagat cttaaagaga
    58441 tcagggacca ggtgggacgg ggtcccgggt gggtgataca gctcttcagg cctggagaga
    58501 cccttgatct gtccctgcgt gaagggactg ttccggacag gggccttctg gagagtaatt
    58561 tcccgggagt attggtgcgt taaaggtcat gagagtataa tattacctgg agtaaaccct
    58621 gaaataacgg aggaacggag aaggcaatag tggctggttt aacctcttcc ggccggggcg
    58681 tcctttctcc agggaaaaga ggtgaacggg accttaagtg cagctatagt tactggtgcc
    58741 ttttggcggc ggtttggtgt agaatcatgg ttgcggtgta catctatctg gaggtgagca
    58801 taagtgaaga aaggtctatt gctagggctg atcgttgtaa ttgcggtttt tggtttcatg
    58861 atgaccaggg gagaaaaagc tcccgaggct ccggctcccg aacagactca ggaagcacag
    58921 gaaccggtag ccgaggccga tgtgatccgc atcggtgtct atctccctct gacgggacag
    58981 aacgcttttg gcggacagct tgagctcgat ggagtcaaga tggcccacga ggagaaaccc
    59041 gaggttctgg gcaggaaggt cgaactcttt gttgtcgaca acaagtcaga caaggttgag
    59101 tccgcaaacg ctgtcaagcg gcttatcgac aaagagaacg ttgtggctgt catagggacc
    59161 tacggctcct cacttgccat ggccggtgga gaggtggcgg agaaagccgg aattcccatg
    59221 gttgggacat cctgcaccaa tcctctcgtg acccagggca agaagtacat cttccgcgtg
    59281 tgtttcatcg atcctctcca gggcgcgggt gcggctacct atgcccacaa ggtgctgggg
    59341 gccaggaagg ctgctcttct gaccgatgtc gccaacgact atgccgtcgg actcgcggga
    59401 ttcttcgaga agtcctttgt caagcttggt ggagagatcg tctcgaaact gaactacaac
    59461 tcgggtgacc aggatttcac tgcccagctt acagagatca tcagcaaggg tccggacatt
    59521 ctcttcattc ccgcctactt cgccgagggt gccatcatca tgaagcaggc acaggaactt
    59581 ggtgtcgagt tccagatcat gggtggagac gccatggaca atccgcagat agtagagata
    59641 ggcggagacg cggttgaggg tttcgttcac accacgttcc cctatgatcc ctccatgcag
    59701 gacatgaacc ctgtagccca gaagttcacc gatgactgga aggcgaaacg gaccgacgat
    59761 cccaacgtca atgcggccct tggatatgat tcctacatga tccttatcga tgccatcgag
    59821 cgcgccggaa gcgccgatcc cgaggccatc accgctgccc ttgcggcaac caaagacttt
    59881 gagggtgtta ccggtctcac caccatcaac gagacccatg acgcggagaa accgatcggg
    59941 atagtccaga tcaaggacgg aaagaaacag tacatcgcga caatagcacc ggaactctag
    60001 cagcaggttc agtcattacg gcggggtctc ccaagagaga ttttaccggc cggattactg
    60061 cgggggaggg gatcttcctc ccccgccttt tacgtgataa caagggggag ctcatatgag
    60121 cttggcaatg tttgtccagc atcttttcaa tgccctcacc ctcggaagcc tttatgcatt
    60181 gatcgccatc ggttatacga tggtttacgg gattttgagg ctgatcaatt tcgctcacgg
    60241 tgagatcttc atgctggggg cgtattttgt gttctggggt gttacgctgt ttcaccttcc
    60301 ctggatagtt tcggtcatag gagccatagc ggttacttct ttcgccggga tacttgtcga
    60361 cagggtggcc tataaacctc tcagggaggc accaaggatc tctgcgctca taagctcgat
    60421 cggcgtatcg ttttttcttc agaactttgc catagtcgtc ttctcggcaa ttccccgtca
    60481 ggtccagaga ccggactggc tggtaaaggt aataatcgtt ggcaagataa gaatccttcc
    60541 actgacgctc gtcgttcctg tcctttcgtt cgtcctcatg ctcgggctgc tttacattgt
    60601 atataaaaca aagcccggcc ttggaatgag ggctatttcc aaggacatcg agacaagccg
    60661 gctcatggga gttcctgtaa acaggatcat agccataaca tttggtatag gttccgcgct
    60721 ggcggcggcc tcggggatca tgtgggccct gagatacccg cagcttcagc cggtaatggg
    60781 tgtcatcccg ggtttcaagg ctttcatcgc cgcggtgttt ggcgggatcg gctctattca
    60841 gggcgcgatg ataggaggcc tgatacttgg tttcatcgag ataatggttg ttgccttcct
    60901 cccggctctc gccggtttcc gggacgcctt tgctttcatt ttgctgatca taattcttct
    60961 cataatgcct acggggctta tgggtgagaa gctggaggac aaggtgtaat ggacgccaga
    61021 atgaagagaa actataccct taacggcgtt gcggtgatgc tgctgggcat cttcctcttt
    61081 tgggcaagcg gcagtctcga cggttacaag atacagatcc tcaacctcat tgccgtcaac
    61141 attatccttg ccctgagtct taaccttatc tatggattca ccggtctgtt ttcgctgggc
    61201 cacgcaggtt tcatggctat aggtgcctat acgtgctcac tacttatcct tcccgcggcc
    61261 cacaaacagg ctatgttcct gcttcagtca ccgatgtggc cattctcggt aatgcatgct
    61321 ccctttattg ttgcgatagt catcgccgga ctggtcgcgg cattttttgg tgtcctgata
    61381 ggtcttccgg ttctgaggct gggcggggac tatctgggga tagccacact tggttttgcc
    61441 gagataattc gtgttcttgc caataacatg cccagggtta ccaacggggc actggggctc
    61501 aagggaatcc cgtcctattc caatctctgg tggaactacg gatggtgcct cattaccctt
    61561 ttcgtgatca tgaggctggt gaggagcaac ttcgggaata cattgaaggc aataagggat
    61621 gacgaggtgg ccgcaagggc catgggtata gacacattca gataccgcct tacatccttc
    61681 actatcgggg cgttctttgc cggggtcggg ggggctcttc tcgcgagcct tctaacgact
    61741 atcgatccca agatgttcct cttcatactt actttcaatg ttctgatgat tgttgttgcc
    61801 ggaggacttg gatcgttaac gggaacagtg ctggcaggcg tcgtcattac ggtgcttctt
    61861 gaatggcttc ggatcgtcga gaaccccttt tctgttggct caataacggt tccagcaatt
    61921 ccggggatgc gaatggtggt cttttcgctg gctttgctct tcataatcct gttcagacaa
    61981 cagggcatca tgggcaagcg cgagttcagc tgggactggt tctttggtct tttcggcaag
    62041 aggggtgacg cctgatgagt gagcgcagga tcgttcttga aacaaaaaac ctcaccatgc
    62101 gtttcggtgg ccttacagcg gtcagggagt ttgacataca ggtacagaaa ggcaggatag
    62161 tggggcttat aggtcccaat ggagcgggta agacgacctg cttcaacatg atcaccggtt
    62221 tctacaagcc aaccgagggg cagatattct tcgaaggcac agagattaca ggctggccgc
    62281 cgcacaaggt ctgcggcgga ggcatagcgc gcaccttcca gaatataagg ctctttcata
    62341 ctgcctcggt cctccagaac gtcatggttg gcgcgagggt aaggcagagg tcgacatggt
    62401 ggcatgctct cctgaatctc cctttgtact ggaaagaaga aagagaagtc agggataagg
    62461 ccatgttcct tctcgaggcg gttggtcttc aaaaactggc agatgaacag tccggcgccc
    62521 ttccttatgg agcgcagagg aggctggaga tagccagagc cctggcgacc gatccttcct
    62581 tcctgctcct tgatgaacca gccgcaggca tgaaccctca cgagacgcag gagctgatgg
    62641 gattcatccg tgacatccgc aaaaggttcg acctcacgat actgctcata gaacatgaca
    62701 tgaaggttgt gatggggatc tgcgagcata tgtgggtgct tgactacggc gtcaccattg
    62761 cggagggcca gcccgcggag atacagtcca acaccagggt catcgaggcc taccttggag
    62821 aggagttcgt ccgcgatgct taggatcagc gaccttaacg ttcattacgg cggcattcat
    62881 gccgtcaagg gaatatcact ggaggttccc agggggaaga tcgtgaccct tattggggca
    62941 aacggggccg gcaaaagttc tacgctgagg gccatagcgg gattgatcaa aaagagccgg
    63001 ggttctgtcc ttttcggaga cattgagatc ggtggatggg taccggagga aatagtcaag
    63061 gccggtataa cgatggcgcc tgaagggcgc aggatccttc caaatcttac tgtttacgag
    63121 aatctgcagc tcggggcttt cagccgttcg gacacaaagg ggatatccag ggatctggac
    63181 tgggtgttcc agctttttcc aagactcaaa gaaagaacct ggcaaaaagg cgggaccctt
    63241 tcgggtggcg aacagcagat gctggcagtg gggagggccc ttatgggtgc accgcagatg
    63301 ctcatgcttg acgagccttc actggggctg gctccccttc ttgtgaagga agtcttttca
    63361 ataatcgaga ccataaactc ggaagggaag acagtccttc tggtcgaaca aaatgcttac
    63421 gctgcactga aggtggcctc cagagcatat gtcctggagg tcggatccat cgtcctgtcc
    63481 ggaaccggag atgaattgct gaacaatccc agtgtcaggg atgcctacct tggaggatga
    63541 aattaacctg attgcccgga gtttcggctc cgggcagttg ttttatggta tcttcagggt
    63601 cgcggcagga agccaggggg tgaacggcga ttgactgtgt ggaacgttcc gaacgtactt
    63661 agtctttcca gggtttttct ggcgcctctg gtcatggtgt ttctgacgtt gagagggcag
    63721 ttcggtaata ttttcggttt gcccttcggg gatatccttg cggggctggt tttcacgatt
    63781 gcggctctta cggataccgt tgacggatat attgcccgga aacatggcat ggtaacgaac
    63841 ctgggcaaac ttatcgatcc tcttgccgac aagatccttg ttgtggcggc attgatatct
    63901 cttgtacagt tgcagcgcct tcccgcatgg atagtcgtcg ttatcatttc cagagagttc
    63961 ttcgttacag gtttgaggat ggttgccgtt tcggagggag tggttatccc ggcatcccgg
    64021 ggaggaaaag ccaagactgt aacacagatc attgccatcc tgatggttat tttcaatgtc
    64081 ccgggagggt tgcttgcgat gtgggtcgcc atgatagcga ctctctggtc gggaatggac
    64141 tacctgctga aaggcagaaa agtactctcc tgattttcct gtttttcaag gcctcgggtg
    64201 ttctccccgg ggcggtgtta taattccccg aaagagccag aagagatgct ccgtcgagca
    64261 gcattcagcc gagctgaaga acagccatag ggaggttttt tgtatggcaa gaccgattca
    64321 gcccgaaccc ttcaggatca agatggtgga gcccgttacc gtaccggact cgggacagcg
    64381 agaggaagct ctgaaacgcg gacatttcaa catgttcgga ttgacatcca gagatatcta
    64441 tatcgatctc ctcacggatt ccggcaccgg agcaatgagc aagcagcaat gggctgccct
    64501 gatgaggggt gacgaggcct atgcgggcgc cgacagtttc tttgccctca aggattccgt
    64561 cagggaagtg cttggtttcg atcatgtgat ccccactcac caggggcgcg cggcggagaa
    64621 tgtggcattt ggtacactgg tcaaaaaagg tgacattatc cccttcaata tgcccttcga
    64681 taccacaagg gcccacgtct tcaacatggg agccgaaccg gtaaactgtg tgatcgacga
    64741 agccttcgac cccagctccc ttcacccctt caagggggat gtggacattg ccaaactcga
    64801 ggaagttatc aaaaaatacg gcaaggacag gataccccta atcatgatta cggtcaccaa
    64861 caactccggc ggtggacagc ccgtaagcct ctccagcctc agagctgtct cggaggtcgc
    64921 gaaacgggag ggaatcccag tattcctgga tgccgcgcgc atggccgaga atgcctattt
    64981 catcaaggaa cgagagaagg gtttcgctga gatgtcggtt tcccggatcc tccgggagat
    65041 gatggatacc gctgatgcca ttaccgtgtc ctgcaagaag gatcccctgg tcaatattgg
    65101 cggaatgatc tgctgcagga cggaggagat gtaccagaaa ctccttccca gagtgatcct
    65161 tttcgaaggt ttcgcgacct acggggggct ggccggaagg gatctcgaag ctcttgcggt
    65221 tggcctcagg gagatgaccg atgagcagta tatggcccac aggatagccc aggtcagata
    65281 tctcggcgat atcctggacg acggaggagt cccgttcgta aaacctgccg gaggacacgc
    65341 catattcatc gacgcggcgg ctttccttcc ccatattccc cagaaatgct atcccgccga
    65401 tgttctgggt attgagatat accgagaggc cgcaataagg gggatcggtc tgggggccct
    65461 tgctttcgcg actgaggacg agaccaccgg agagatagtc taccccaggc tggaactcta
    65521 cagactggcg atcaacagga ggacctacac aaacagccac atggagtatg ttggagaatc
    65581 aatagtaaac gtctacaggc gaagggattc catccgttac ggtcttcggg ttgcatacgc
    65641 tcctcctgtg aaagggcttc accatttcct tgctcatctg gaaccttttt cgctttaaaa
    65701 ttttttccca gggggcggcc tggccgcccc ctttttgttt cagcatcctt ttttgacctc
    65761 cccactggcg gggaatataa tgtcagtctg gaaggtttcc ccgctttccg aagccgatcg
    65821 aggtgaaatt aatgcttgaa ggattgaaaa agaaacttgg ccttgaccac aacgagaggg
    65881 cgctgaagcg ttacgcttca gtcgtgtccc gcatcaatgg tcttgaatcc tcaatgtccg
    65941 atctttcgga cggagaattg tccgcactgg gaaaaacttt ccgggagagg tgcgccgcgg
    66001 gagagtctct cgacgacatg ttgcccgagg tgtttgccgc cgtcagggag gtttctcgaa
    66061 ggactctggg aatgagacat ttcgacgtcc aacttatggg gggaatggct ctccatgagg
    66121 ggaagatagc ggagatgaag acaggtgagg gtaaaaccct tgtggcttct ctcgctgtcg
    66181 tgctcaatgc ctttacgggc agaggcgttc acctggtaac ggtgaacgat tacctggcca
    66241 aacgagatgc cgagtggatg gggcctatct acaggttcct tgggctttct gtgggagtca
    66301 tatactcttt catggatcag gccgagagga tcaaggcata cagcgcggat gtgacctacg
    66361 gcactaacag cgaattcggt ttcgactacc tgagagacaa catggcggtg cactcggcgc
    66421 aactggtaca gcgagaacat tctttcatta ttctcgacga ggtggactcc attcttatag
    66481 atgaggcgcg gactcccctg atcatttcag ggccatcgga ggacaacgta gaggtttacg
    66541 tcagggccga tgaagttgcc aggcgtctgc gcaagggtga agattttgaa cttgaagaaa
    66601 aggaaaggaa cgtagccctg accgaggcag gtatatccag gtgcgagaag atcctcgaga
    66661 taccggaact ttttaccgat tacaacaggt cggaactagg acacagaata atccagtccc
    66721 tgaaggcaca tcatcttttc cagaaggata tccactatgt caacaaggac ggggagatag
    66781 tgatcgtcga cgagtttaca ggccgtctca tgttcgggag acgttactcc gacggattgc
    66841 accaggcgat agaggccaag gaaaaggtgc aggtcggacg agagaaccag accctggcca
    66901 caataaccct tcagaactat gtcaggatgt accggaaggt cgcgggcatg accgggactg
    66961 ccgccacaga atgggaggaa ttcaaggata tctacggtct tgaggtattg gtaataccta
    67021 cccacaagcc catgatacgt gaggaccggc ccgatgttat cttcaggacc cagaacgaga
    67081 aatttgctgc cgcagcggac gagatagagg agaccttcgg gacgggacag cctgtgcttg
    67141 tcgggaccac atcgattgaa agttccgaga ggctgggcaa gatgctcaag gccaggaaga
    67201 tagaccacag gatcctcaac gcaaaatacc acgagatgga agcccagata gtcgcccagg
    67261 caggccgcct gggggctgtg acggtggcta ccaacatggc cgggcgagga accgatatcg
    67321 ttcttggcgg caaccctctt tttctggcaa gggaagaggc ggcaaggcag ggtttcgatg
    67381 aaaactccca gcccgaaaaa ttttcccgtg ttctggagga aaaacagaaa gagtgcgaaa
    67441 aagagcacgc cagggtcgta gagctcgggg gactcaagat aatcgggacc gaaaggcatg
    67501 aatcacgaag gatagacaac cagctgcggg gcagaagcgg acgccagggt gatccgggat
    67561 gcagcacctt ctatctctcc cttgaggatg atcttctccg tcttttcggg tctgacaaga
    67621 tcagcggttt tatggaaaaa ctgggtctgg aggaaggaga gtatatagaa cacggcctcc
    67681 tgaccagagc catcgaatct gcccagaaga gagttgagga gttccatttt gacatgagaa
    67741 ggcagctgct catgtacgac aacgtcatga accagcagag agaggcgatc tacaccgaaa
    67801 gaaggcggat cctctccgag ccggatctga cttcacatgg cagacaactt atagaggatg
    67861 ccctcgacag catactcgag agtttttttc cggaagaagg ggagcctcag ccccaggcag
    67921 tggcccttca gctgaagggt gttttctggc cggggatcga gaggcatatt tccggtgttg
    67981 aagaacccga agacctggag tcggcccggg aaaatatcat gggggatctc catgaaaggt
    68041 tcgaagaaaa attcagggac ctgggtgatg aaaactctac gggaatgatc cgtttcctcc
    68101 tcctcagcgt tctcgattcc gcctggaaag aacatctcct gtcgatggat gagctgcgaa
    68161 gggggatagg gctcagggcg atagggcaaa aggatccgct tcttgaatac cagtttgagt
    68221 catataatct cttccagagt atgatggaga gggtccgtat ctccttcgcc gactatttct
    68281 tcaaggtcac cgtgatgacg ggagaaactt ccggaaagac tccggcaagc cggggcaggg
    68341 agtcgcggga aaatttgctc tccctcccgg gaagcgcggt gccgaatacc cagaccggaa
    68401 agcctcccgc aaggagcgtt aagaaaacag gcaggaacga gccttgtccc tgcgggagcg
    68461 gcaaaaaata caagcattgc tgcggcagaa acatttgaaa gagatgatgg agagcagcct
    68521 attcctggag aaatgatggg agtgagtttt ttgagaccca gaacgatttt ggccatgatg
    68581 ttactggttc tatttatggg cctctgtggt gttgcgaatg cccgcgatat acccgcagag
    68641 ctggcgagat cgctgaggga agcaccttcc ctggaatctc tcggctctgg ccagggcctt
    68701 gtgctccttc gcgatatcag ctacagactg cttgcggacg gaagaatgga gagaaccacc
    68761 ctatggttca tccatgaaga gaaggggctg cccgacagct ggagaaaatg ggaggtcgtt
    68821 gtccccgaag gaggtgaggc ctccgtcgcc gaagctggtc tgtatgaccc cggatcaggg
    68881 aagatacagt atccccttat acccagggaa acatccagag acggggcttc ccttatcgag
    68941 gtaagggttc ccaacagcct ggacggaaat atttttgtcc tgtcctacag gcaggtcttc
    69001 cccacaagga tgaacatcga ggactctgtc ctagtggatc ttgatattcc ccagtgggaa
    69061 cagaggataa ccctgaaggt tccctctggc actcttcctg agtggagcgg agaaggtatt
    69121 ccggacccgc aggtggaaaa agggagttcg accgacagtt attcctggtc gatagtcaat
    69181 acactggcaa gggaaaacgg caccctttcc gccgaaccgg gaagggtcct cgtcctgagc
    69241 cttcggaagg ggcttcgtta ctccctggca gaggcgatgt ccgtggctga ttcaatcggg
    69301 gtgtcacctc cttcgcaggt ggtctccatg ttgtctgacc agaacaggac caggggggga
    69361 gaacgggtca ttgatttcgt caacgaccct tccctgatca tcagaggcct ccccgagatc
    69421 ctggcaaggc cggttgaact tattccggcc gaaggaccct ggacagaatg ggaggcagca
    69481 ttcctgctgg cgaactggct taagaaagcc gactgggggg ctgatatact ctgggaggcg
    69541 cgaacgaccc ttagggacga ttcgcccgct acgataacat ctttgagaaa gccggttctt
    69601 gcacttactc ctccgggggg aaaacagttc atgttcgaga tcggtcaggg ggttaaaccg
    69661 ggacaggtgc cgccccgtct ctggggaagg actctatatt ctcttgacgg gaacacagta
    69721 ctgagacgtc agataccggc aggagggccc gcagaccaca ggctttcctt taactggaag
    69781 ctgggtctct caccggaagg ctttgcagag ggagatctgg tcatcaatgt caggggagcc
    69841 tgggtcgaca ccatctccgc cgggaatctt cccgatgaga aacaggccct ggatatgctc
    69901 atggcttttg gatggccatc cacgcctgga atgcccggag gtgaagctgt tgtggaacct
    69961 gctggaagcg ggttcaggat cactctgccg gtcagggcac agatgggaat accctcaggt
    70021 gagggtttgc tgatgaggat gccttccgct ctgatgccat ggcagatgat cattgccgag
    70081 aagggggttt caggggggat aaggttcccc tttgtttatg aacaatcgat agaaatagaa
    70141 cttccggaag gctttgaagt catggttctt ccggtcctca ggcctgtgga agcaggctct
    70201 gtgaaaacag gagagagcct cagggtcaaa aaaggcagga ctctggtcgg agagcagaag
    70261 gtggttatat cgtcggcaag aatggatgat cagaccaggc aagtttttgc caactccatc
    70321 cggcagggtc tgggctggtc gggtatcacc gttccaatga ggaaaaggta gcgaaacggg
    70381 gtgagatcat gaaaagggat attactgaag tattgaggga aatgctcgaa agatccatag
    70441 gtaaaatggc ttgcgagagg ggtatcgagt tcagggaggg gcccggaata gagattgaga
    70501 ggacaaggcg cgagggccag ggcgactggg ccacgaacgc tgccatgcag tgggcgagga
    70561 tcttcaggga aaaacccctg tcaatagcgg agaagatcgt ttcgggtctg gagggtgatc
    70621 cccttatcga aaagatcgaa atagcggggc ctggttttat caatttctac ctttccgatt
    70681 cctggatgtc ggggatcctc ggccatattc tggaacatgg agatgacttc ggacgtgtgg
    70741 acacgggagg cggcaggaag gtgcaggttg agttcgtcag cgcaaacccg acaggccccc
    70801 ttcacgtcgg acacggcaga ggagcagctg taggcgatat atgcgcccgg atactatcct
    70861 tcgcgggatg ggatgttcag agggagtatt acatcaacga cgcaggtctt cagatggatc
    70921 ttcttggccg ttcaacccag gcaaggtatt tcgaaatagg gggttccccg gagaaggccc
    70981 cattcccgga agacggttac aagggagact atatctatga tcttgccaga gatgtgatga
    71041 attccgaggg ggacaggttt ctcctggagt ctccggatga ttcctttcct tttttcaagg
    71101 attttgctgg aaaagttatt cttgatggta taaagaagga tctttctgat tttggcgtcg
    71161 aattcgatgt gtggttttcc gagagatccc tctacgatgg agtcagggtt cccgcaatgc
    71221 tcgaaacgct gaaaaagaac gggtttgcct tcgattccga gggagcgacg tggttcaagg
    71281 cgactgcttt ttccgatgac aaggacaggg tgctgataag gagcaacggc gtcccgacct
    71341 acttcgcttc cgatatcgcg tatcacaagg acaagtatga cagagggttc gatatggtta
    71401 tcgatgtctg gggcgctgac catcacggct atatccccag gatgaaagca gcagtccagg
    71461 ccctcggaag gaaagagtcc gatctgcaaa tactcctgat ccagttcgtt aatcttctgc
    71521 gcgggggtcg ccaggtttcc atgtcgaccc gatcgggaga gtttgtaact ctcagggagg
    71581 tcattgacga ggttggggta agtgccacgc gttacttttt tgcgatgaga cgatcggaca
    71641 gtcatctgga ctttgatctt gatctggcaa agagttcttc taacgaaaat cctgtatttt
    71701 atgttcagta tgcccatgcg agaatacaca gcatcttaag ggaagccgag tcaagggaga
    71761 caattgtgcc atctttcgat gaagccaata tttctcttct tgaagaaccg gaagagatcg
    71821 ctcttatcaa gaggctttca gcgttccccg aagaagttga aaaagccgcg gatgacctgt
    71881 ctcctcacag gatcgtttac tacgcctatg atctcgcggg agatttccac actttctaca
    71941 acgctcatcg tgtcctgggg gttgagccag acctggagaa agccagactg cttctctccg
    72001 aggccacgcg cattgttctt tccaacgcgc tcaatcttct ggggataagt gctccggagc
    72061 agatgtagag atgatccggc ttcgctgggt cgcgcttatc gccgcggggt tgtgtttcct
    72121 ggcaatagtc ggaactgcgt atgtgcttga gctcagaaaa ataagcaggc tgagcggtat
    72181 catcgacgag cgaatggaca ggcttgtaac aatgacaaga gatgtccaga tacttaagga
    72241 aaagatcatt ttctacagga cacctgaagg agtggcgagg ctggccagag agcagttcaa
    72301 ccttacctat cccgatgagt ggatatttag aattgagata gtgtccgaag atgccttgcc
    72361 gcaggaaggg cattagcgta taataccctg ctgtatgtcc ctgtctctcc aggtgaggag
    72421 agaccatagg ccatggggag gaggtgaaat acgtgcgacc ttacgaaatg gttacaatct
    72481 tccaggctga cctggaggat cacaagaccg ctgttgagga tatcgaaggg gtcgtgcgca
    72541 acctgggagg agagatcgaa aagagcagtt cctgggggaa aaggcgtttt gcctatccca
    72601 tccagaaaca gaccgagggt ttttatgcgg ttctcaattt cagtctcgac ccctcgcagg
    72661 ttcgcgaact tgagcgtctg ctgaagctca aaacacaggt tatacggcac ctggttatcg
    72721 ctcttgaaga agcctaggga gtgattctga tggcaagagg attcaacaag acgatattga
    72781 tggggaacct tgcccgtgat cctgaggtca ggtatacgac ctcaaaacag gctgtggcaa
    72841 aaatgacggt ggcagtcaat cgccagtgga aaaacaagga cggcgaggtc caggaccagg
    72901 ccgactttat tcccgtagtt gtctggggat cccaggcgga gaattgcgaa agattccttc
    72961 gaaaggggag tcctgtcctt gtcgagggga ggatacaggt tcgcagttac gaagccggat
    73021 cgggcgagcg acgatgggtt accgaagttg tggcccaggg ggtgacattt ctcggaaccc
    73081 gaaaagatag cgatggttat tcccaggaga cggcccaggg atccgggagc aggtcaggga
    73141 gcctcaggga aaaaggtttc ggcgatgatt ttgttatgga catatccgaa gtcaataatg
    73201 atggagaaga aggggaggaa gctgatattc ccttctagca gggggcggtt caccgcccga
    73261 aaggaggatc gcgatggcct ttacgcctcg caacaattct tcaaagcgca caaagagaag
    73321 aaagcccaag gtgtgccagt tctgtgttga caaggttggt cacattgact acaaagaggt
    73381 tgataaactc aagaagtttc tcagtgaaag agggaaaatt gtcccgagaa gggtcactgg
    73441 aaactgtgcc aaacaccaga ggcagctcac ggaagcgata aagcgggtac gttatctggc
    73501 gctccttccc tatagcgccg agtagtctga ccgctaaaga ttttttcggg gggagaggcc
    73561 atggcttctc ccccctattc gttcaggagg tctgctatgg acagaaccag ggaactggta
    73621 gaatcctctc ttcttgtcgc tcttgcagcc gctcttttcc tcgctggcaa ctttcttccc
    73681 ctggtaggga tggccttttc gcttgtttgc ccggcaccgc tggtgattct gggactcaga
    73741 cacagccttg gcagatcgat cctgggggtg atcgtggcca cggtaattgt ggcggtattt
    73801 accggcgtcg taggagctct cttcttctgt cttggtttcg gttccctggg tatcgccctc
    73861 ggtttttttg caaggcgcta cgacagggct gtggatataa tactctacgg aattctcgta
    73921 tccctgggga gcaagctcat cctgatgttc ctggttgtga aggtgacggg aataaacccg
    73981 ttttcgctgg atgaggcaga gttgctttca gtaatggaca agatcggctc cgtatattct
    74041 tcgatggggt tgccggaaga cgcggtgtcc tcggtttccg aacagatgcg attggccctg
    74101 ggccttgtac ctgttatttt cccgtcactg cttgtcatgg ccgccacagt ggactgtttc
    74161 ctcagttatt tggtcagtgg agtcgtactg aaaaaactgg gaaaggagag agtccccgag
    74221 gttccgggat tttcggagtg gcgtttcccg aaaagcattt tccttgccct tctcgcatcg
    74281 atagcgatgc attttgcggg ttcctggtat ccctcacttt ccctgtttca gagggcggga
    74341 ctcaatctgc gtcttctcgt cacggttctt ttctttatcc agggtcttgc tgtagtgtgg
    74401 agtttcatga gagccagggg gctaggccgt ttcctgagaa taacatttac catgttgata
    74461 attcttgtat cttttcttgc gcagatagcc ctgatattgg gtatcattga catgtggttt
    74521 gatctgcgca caaggttccg ggggggaaaa gcatgaaggt cattcttttg agcgatgtgg
    74581 caaaacttgg caggaagggg gacctcatag aggtctccga gggttacggc aggaactacc
    74641 tcgtgcccag gaagatggcc gaagaggcca ccagggagaa acttgaggag tggaaacagc
    74701 gccagaacgc tatggaaaaa aaagccagga aggaagaaga agaggcgcga acccacagga
    74761 acttgatcca gggtaagcag gttgtcctca gggcgactgc cggagacaag ggcaagctct
    74821 tcggcagcgt gaccgctgcc cagatcgccg acggcataaa ggagaaattc gggatagatg
    74881 tggaccgcaa ggatatccgt cttcctgggc agatcaagga ggtgggctca tatcctctgt
    74941 cgataaagct ttattcaggg gttgaggccg aaatgaccgt tcttgtggag gccgaaaaag
    75001 ttgagtgacc gcctctttga gaggattcct ccccatagta tcgaagcaga aagggctgtc
    75061 ctgggtgatt gtctgcttga tcaggacgcg ctgaccatta cccttgatta ccttcttccc
    75121 gatgattttt acgatgggag ccacaggaaa gccttcagcg taatgagaga gatggcagag
    75181 aagggtaagc ccgtggatcc actgaccttt ctcgatgaac tttcaaggag gggtcttgtc
    75241 gagtctctgg ggggacaggc ctttattgcc gggtttgtag attcggttcc aacgacggcg
    75301 aacgtggagt accacgtcag gattgtaaga gacaaatctg tccatcgcag gcttatacag
    75361 gtcggggcgg agatagcaag gctgggatat tccgaggaca gggaacttga ggaagtcctc
    75421 gacgaggcgg agagatccgt ttttgaaata tcacagcgag gaagccaggt tccataccgg
    75481 agggttgggg atgttcttgg tccggcattc catgagatcg aggaaaaatt tcattcgccc
    75541 gacagttctg tgacgggaat accaagcgga ttcagtgatt ttgagaggct gacgggggga
    75601 ttccagccgg ggagcctgaa tatcattgct gccaggcctt caatgggtaa gactgccctt
    75661 gctcttgata ttgccatgaa cgcatccgtg gaaagggaca taccggtcct gatgttcagt
    75721 cttgagatgg gttcggaaca actcgtccag agaatgctgg gttccagagc gaaggtcaat
    75781 attcatgatc ttcggactgg aatgttccac gagaatgcct ggggtgacct ggcccaggcc
    75841 gcgggggatc tttccagggc tcccttttac atcgacgaca gttccgtgct atccaccctg
    75901 gagatgaggg cgagatgcag acggttcaaa tcgcaggtgt tgaaaacggg gggaggactg
    75961 atcgttgtgg attacctcca gttgatgcag ttctcccgca ggatagagag caaacagcag
    76021 gaagtggcgg aaatatcgag atccctcaag gcaatagccc gggaactgga tgttcctgta
    76081 attgcgcttt cccagctttc cagggcggtg gagtccagga acgagaagcg gccacagttg
    76141 tcggatctga gggacagcgg cgcgatcgag caggatgccg acctcgtggt gcttctttac
    76201 aggtcgggtt attacgaatc tgcccaggag gaggatgacg ccaccgccga ggtgatcatt
    76261 gccaagcaca ggaacggtcc gacgggaacc gtaaggctga ccttcttcaa ggagcacgcc
    76321 aggttcgcaa accaggcgtg gagtaactga ggaacgggcg gggtctgtca tggagctcaa
    76381 cggactgctc aaagtcagtc ccgaaataat tgacagggat cccgatcagc cgcggagaat
    76441 attcgacgag gctgctctga aagggctggc aaggtccata accgaagtgg gcctgcttca
    76501 accactcctg gtacgcggta aaggtaacag gttctgcctg atagctggag aaaggagact
    76561 tattgcctcc aggatggctg gactggattc cgttcctgtg gtcgtggtcg accccgatcc
    76621 cgctctgcgc tcggtcctcg cactggtgga aaacattcat cgcaaggacc tttcctcact
    76681 cgaacaggca aaggccattg gcgggataat agaaaaaacg ggttggaccc agtcggagac
    76741 aggcaggagg atcggacttt cgcagccctc gatagcgaac aaaatgcgcc ttctcgatct
    76801 ggatgaaagg gttcaggaaa tggttcttga tgggacattg ggagagcgtc atgcaagggc
    76861 actgatacca ctttcccctc agatacaaag ggagattgcc gaaagatccg tccgggaggg
    76921 tcttactgca agggccatcg agaagatcgc tgcccgtgcc cgtaaaggca ccgctgatga
    76981 cgacgtacag aaaaaagcat ccttgctcca gaaggagata atgtccgaga tctccgaggc
    77041 tgtacggaga aggcgggaaa aggggctcga tgtaaggatg aagatcagaa ccgtcgggag
    77101 ccacaaacgc agactcgaga tcctcgttac aattgaaaac gaagaggctc ttcagtgacc
    77161 aggggcaagg cagaagaacc ggtcaccggg gatatgttct ccttgaataa tggaaccgta
    77221 gttaccggcc tcctggctgt cgctcttgtg ttgccgaacc tgcttttttc cgggacctgg
    77281 tggtatgaat cgctccatat catcaaatgg gcggcggtct ttatccctgt tggtcttctt
    77341 gtttttgcgg ccggataccg tatcgcatca ggacggggga gggatccggt cctcgaccct
    77401 ttcggagtcg tgtggctttt cctggttctg ctccttctca tgcagcccct ttgggctcct
    77461 gtaagatcaa tccctacctt tgccagggag tggtttttct tcgcggccct gtggggggtc
    77521 tatgtggtgt caaggggcgg tctgaagaag ggcagcctct ccctgtttct atggggagcc
    77581 tccttcaatg gggcgataaa cgtacttttc gccgaaatcc aaataagggg actgcagggg
    77641 atcttcccct tcatataccc tgtgtctggc ctgtacatcg gaaacactgg ccagcagaac
    77701 atgtttggac tctggttggc cattgccatg ttcggctcgg cgagcctgtt ttacaatact
    77761 gccgataaag gccagggcaa gaactcgttg cctctggcgg tgatcctgaa tcttcttctc
    77821 ttctcgatct gttcctgggg gctgtggaat actaccagca gatctgccgt tctgtctttc
    77881 ctggtgggcc ttgtagttat gacattcctg gcagtacgaa acagcaacag gagggctatc
    77941 ctcctgaggc tggccgctct tgcggcgatc atggccgtga ctttctcggg aacggcctat
    78001 ttgggccgag ggaacgcctt tattatgaag acccgggaca tgatcgaaaa ttttcagaca
    78061 gtagggaaaa gggatagtat ctgggcagct tcggggacga tgttccttat gcatcccgta
    78121 agaggggtgg gtctcgggca tttcaagtgg aactaccttg aagcccagag agagatgctc
    78181 ctcagacatc cgggaatgaa gtggcagtat acatactggg cccacaacga gatactgcaa
    78241 tggttctgcg agacgggtat agtgggagga ctcatccttc tggcaatggg agtatggtgg
    78301 ctcagtgctt tcttttctca cgtacgtaaa gggaaacccc tgtcccggga ggccatgtgg
    78361 gcttgcagtt ttcttttcct gatctggttc aacgctcttt ggaccagacc ttttcacagg
    78421 atagaggatg ccgtatggat ggccctggct tttgcccttg ccaacagaga gatacttcca
    78481 tcaagggccg aatggacaga gataagaagg ggatgggtcc tgaggggtct tggagttgcc
    78541 atggcagcgg gggcgctttc cggtcttttc ttccttggca gcggtatggc cggggatcgc
    78601 attctcaggg ttgccgcctc cgaaaaatcc atgccagaac agaggctgct cctcgaaagg
    78661 gcttcccgtc gactgatggt aagtgacatt gccgagagaa gactggctct tcactacatc
    78721 agacttgccg aggaaactaa aatggggcaa tacctggacg aaggtctgac caggcttcac
    78781 ctgtattttc tccggcaacc tcatgttagg gatatgataa ggctgatagc gtggtactcg
    78841 gatgtcggcg aggctggcct catggaaagt gttgccaggt atttgcggcc gggaagttat
    78901 acgataacag gggggaaaat aaacctgagc tccgaggacg agcggagatt tttcggccag
    78961 tcagacaacc agtacacaag ataatcaggg gcggccgcac aaaaaacggc cgccctgaat
    79021 ttttttcaca tgagaaaaac ggttttcacg attttctact ttcccatcaa catctcgcag
    79081 taggtcgggt atggccagaa cccggtcgag acgatcacct ctgatgcatc acacatcccc
    79141 ctgatgccgt ccatgagggg gattgccttt tccgtcagca cggaggcccg ctgttccagg
    79201 tccagcgacg atagactgtt ccgtagagac gccatttttg caactccgtc aagcagcctt
    79261 tccttcatcc tgtagagttc ctcgatatgt tcctgccagt gtgaggagga acccgcccgc
    79321 gcgctttcag ggacatgggc caatgctgtg ccttccgatg tgatatgact ggaaattgcc
    79381 ggaagaatgc cgtttcggac catactctcc attacgttca tttctatatc gagggtcttg
    79441 acatactgct caaggcgggt ttcgtagtac ccttcgatct ctctttcggt aaagatgccg
    79501 agttttgaaa gaagttccct gtgttccggc acgaggaaaa gcgcaagggc ttccggcgtg
    79561 ctgtgagcta tcggcagtcc ccttttttgt gcctcatcgt gccactcggg tgaatagcaa
    79621 ttaccctcga aacgtatgtt cctgctttcc atcgcggcat aacggatcgc gtcgagggcg
    79681 gcatcggcaa catcggtctc ccctatcctg gattctatca tgttggcaag ttcttccatt
    79741 ccccatgccc acattgaaag aagcatggtc ataggtccgg ccggagactg ggatgatcct
    79801 ggggccctga actcgaattt gttacccgtg aaggctatgg gagcggttcg gttcctgtcg
    79861 gtgttatcct tgagaacatc gggaagacga ttcaggccaa ggtcgataaa ggccttggaa
    79921 ggcatgtttt ccggtattcc ttcctcgacc ctttcgaata ttcccgaaag cacatctccg
    79981 agaaagacac tcatgatgga aggcggggcc tcgttccctc cgaggcgcct catgtttccc
    80041 ggcgtggcga ccgaggccct cagaagccct ccatacctgg aaatacccag aagaagagct
    80101 gaaaccaaag tcaaaaactg gatatttttt ctctggcttg atgaggtttt gaggaggttt
    80161 cgaccctcgc tatccattag ggaaaagttg agatgcttgc cgcttccgtt gatcagggca
    80221 aaaggttttt cgtgaagcat gagcctgaag ccatggctgg aagctatctg ccgcatctgc
    80281 tccatgagga tctggttctg gtcgacggca aggttggcct cggtgaactg gggagcgaac
    80341 tcaaactggc acggtgctac ctcgttatgc cttgttttca ggaccatgcc cagcgagcac
    80401 atacgtttgt cgacatcttg catgaaagcc agtaccctgg acggaatcga accgaagtag
    80461 tggtcctcca tctgctgacc cttgggggta ggggctccca gtagagtcct gccgcagtac
    80521 tggatatcgg gtctttttac cgcacaggat tcgtcaataa gaaagaactc ctgctctgct
    80581 cccattgtag cgcgggtcca cttgacactc ctgtttccga aaagtttcag cacccgcaat
    80641 gatatgttct ctatccgcga aagagctctc agcagcggag ttttcatatc cagaggcgta
    80701 ccatcgttgc ttatgaagac agaaggaatg cacagggttc ccccatgttc cgccgggacg
    80761 acgaatgccg gacttgtggg atcccaggca gtatagcctc ttgcctcaaa tgtcgatctc
    80821 atacccccag agggaagtga tgatgcgtct ggctcgctct ggataaggtc ggccccccca
    80881 aatgaatcca ggggtatccc gtgttcgtcg atggtaagaa aggccatatg tttttccgcg
    80941 gtcaggtccg ttctcgggtg gaaccagtga gtatagtggg tagcaccaca ggaaactgcc
    81001 cactccttga tggctgtcgc gactatctcc gcaacgtgga aatccatctt ttctccgccc
    81061 gagatagcct cctgaagttt ctggtaaacg tgtttgggga gtcgttccct catggccctg
    81121 tcgttgaaaa cgttgctccc gaatgtcttt gccggagatt ccctcattga tatcacccct
    81181 tgttgatgat aagtgtatat atcatcaaaa gagaaatttt gcaaccaaaa acaactattt
    81241 ggtgcgtaat ttttgtgttg ttgttgtttt tggaagcaaa aaggtgtgta tacacgcaaa
    81301 aaacaggttt ggctcatcaa aaaggttttc tgagggcttt catggacagg tgaatatgat
    81361 ctctcataag ccgttcggag agtttttcgt cgtgcatggc aagtgatctt actatctcgc
    81421 gatgttcgtc caggctgggg tttgtatcaa aatcgaagaa tgattcgaaa aacaccatgt
    81481 aaacgtaggt ccttgacaga atgttgccga cataatcggc aaggatcctg ttctttgaag
    81541 accccgcgat tattctgtgg aagcggatat taacctcaag atatttttcg agatctcgtg
    81601 aggaaaagat aacctcctcc tcttctatcg cttcgttgag catgcatatc tgcaacgggg
    81661 taatgaaacg tgcggcgcgt ctcaccgaga tgctttcaag ttcctctctg acctcgtaac
    81721 cttcggtcat ctcctccaga gacggatttg ccagcctcgc tccgctgttg ggtgtaatcg
    81781 tgacaagtcc ttcggaggcg agcattctga gggcctccct cacaggggtg cgacttacgg
    81841 aaagactttt ggccagagag gcttcgggaa gcctctgtcc cggaagcagt tctctggcga
    81901 aaatcctttt ttttaattcc aggtagacct gttcagaagc tgtagcgttc tctccattag
    81961 taatacctat catcctggtt cccccgatgc agtttgcctg atattttacc tttaaaaaac
    82021 cggtgggcgg tatttcagat gttgactgaa atggatatgt gcgtttagta tacgtagtat
    82081 accacgtata ctaaaaggag gtatcccatg tatccggatc tttcgggaaa aaggattgtt
    82141 ataacaggag gagggagcgg gataggcctt gcaacggccc gacggttcgc cagagagggg
    82201 tccagggtct gtgttataga cagggacagt tcttctctgg atcgtgcaag agaagaactt
    82261 gccggtgagg gccttttctt gtgtgccgat gtttctgact catcatcggt cgatacggct
    82321 tttggccgga tcgacgaatc aatgggaggt cttgacgtac tggtttccaa cgcgggaata
    82381 agcgtcagga aaacattcag ggagataacc tatgagcaat ggtccaaagt tctggaagtc
    82441 aatctcggag gaatgttcct ctgcgccagg gaagccgcgg cgaggatgaa agacggtggg
    82501 gtgatcctga tgaccgcgtc caccaacgga atggaggggc atccctttta cgcagactat
    82561 aactcctcca aggctggcgt cattcttctt gccaagaccc tggcgctgga attcgccccg
    82621 cgcattcgcg ttaacagcgt cagccccggg tatgtcctga ccccgatgca gagggccgag
    82681 tacacaccgg aaatgcttga aaaggtcaac gaaaagatcc ctttcaggcg ccacgctgca
    82741 ccagcggagg tggcggctct attcgccttc ctggcctcca gtgaagcatc atacattaca
    82801 ggagcgaaca ttccgataga tggcggagag accgccggac ttcaataggg ggtttgtgaa
    82861 tatggagcac gaagaattat tcagactgga tggaagaacg gctgtcataa ctggaggagc
    82921 atcggggata ggtctcgggt gctgcaagtt tctatcaatg atgggtgcct cggtcgtcct
    82981 tgccgatctg gacgaaggta aagggcgcac ggcagagggc gagatcgggg gaagatctct
    83041 tttcgtcaag tgcgacgtaa ccagtgatga agactgtcgc aacatggcat ccgctgccgt
    83101 agcacgtttc ggaagggtgg attatctcgt aaactgcgcg ggtgttatca ggaggaagaa
    83161 cgttgtggac cttacagaga aggagtggga cctgaccctc gatgtgaccc ttaagggggc
    83221 tttccttgcc tcgaagtatc tcatcccctt tatggctcga atcggagggg ggagtattgt
    83281 gaatatagct tcgggatggg gcgtaaaggg aggtcccagg gcagtgtcgt actgtgcggc
    83341 aaaaggtggg attatcaaca tgaccagggc gatggctata gatcatggtc atcagaatat
    83401 cagggtcaat tccgtttccc cgggggatac cgatactcct cttctgagag atgaaggaag
    83461 gcagttgggt gtagatgagg ataaatggct ggccgaaagc gcggagcgcc ccctgagaag
    83521 gcttggccgt ccagatgata ttgccatggc tgtttatttt ctggtaagtg gcctttcccc
    83581 gtggataacg ggggcaaacc tggtcgtcga tggcggcggg atcgcctaga gatcgtgtac
    83641 aggaggtttt gaagatggaa gaaaaaaaga gagtttatcc atatatcccc aactccgaac
    83701 ccggggtcag ggagaccatg cttcgggaga taggggttga aacaatcgat gatcttcttc
    83761 cggatgtccc tgactcgatc cggatgaagg gaaggatgaa ccttcctgat cccctgtgtt
    83821 ccgaaccgga cctcaggcgt catgttgaag gtctcctctc agagaacgtg acctgcgggg
    83881 agtgtctcag ttttctgggc tctggctgct acgaccatca tgttccagcc gtagtcgacg
    83941 aaataatcgg acggtcggaa tttctgacag cttacgctgg cgaaccctac gaggaccacg
    84001 ggaggtttca ggcgcttttc gaataccaga gcatgatgtc cggactcatg gattttgatg
    84061 tgtgcaacgt gcctacctac gacggctccc aggccgcggg tacctctctg agaatggcgt
    84121 caaggatcac cggcaggaaa aaggttctcc tcgcggggaa catgaacccc gacaggatga
    84181 ggatagtgaa gacctatatc gatgaggccc tttctccaga gttgatctca tggtcgcccg
    84241 atacgggcct tgtcgacctg caggatctgg ggcgtaaact tgggcccgac gtcgcggcag
    84301 tatatattga gaatccctca tacctgggtg gtatagaaca caggggagcg gaaataggcg
    84361 ccatgaccca cgacgcgggc gcgatcttta tcgtctcacc cgacccctct tcgttgggaa
    84421 ttcttcagcc accttcgaga tacggagccg atattgcctg cggtgatatc cagtccctcg
    84481 ggatccatat gaacttcggg ggaggagtgg gtggatttat atgcaccagt tccgacagaa
    84541 ggtttgttga agaatatccc tcgcggcttt tcgggatcgc gcctaccagc aagggggaat
    84601 ggggctttgg agacgtagcc tgggagagga cttccttcgc gaacagggag actgcaaagg
    84661 aattcgttgg aacccatgcc gccctctggg gaattgccgc cgcggtatac cttgcgtcta
    84721 tggggcctca aggaatggcg gacctgggga aggtgataac caggcgatca ctttatgcaa
    84781 ggaaggtgct cggagatctt ccaggagtgt ctactgccag gccttccgct gtttgtttca
    84841 aggagtttgt tgtcgatttt gctggcaccg gcaagactgt ggccgagatc aacgggaaat
    84901 tgcttgaaaa aaggatattt gggggaaagg atctttcttc tgattttccg gagcttggcc
    84961 cttgtgctct gtactgcgtg accgagaaga taaccgtgga tgatattgac aggcttgcat
    85021 ctgccctggc tgagataatt gcctgacaga aaatgcctgc gagaaaggaa tgacggacaa
    85081 tgactgagaa tctcaggccc tttcaccata tgaggtggaa tgagcccata atctaccagt
    85141 tgagcgagcc gggacagagg gggatcctcg ttcctgaggc cgaggagaag atcaccgcga
    85201 tggctggtga cgggatgtcc ctgataccct ccgcaataag gcgaagcgat cttccggatc
    85261 ttccggaaat ggcacaaccc caggtggtaa gacatttcaa ccgcctttcc caggagaacc
    85321 tgggtgccga ctctaatatc gatattggcc agggaacctg cacaatgaag cacagcccga
    85381 agatcaacga aacttttgcc cgatccccaa agataacgga ccttcaccct tatcaggatg
    85441 tgtctacagt gcaggggatt ctcgagattt tctaccggat ggaggagatc ttcagggaga
    85501 tctccgggct tgatttctgc agtgtccagc cggggggtgg cgctcatggg gccctcgcgc
    85561 tggcttcgat agtcagggcg tggcatcgtg acaggggagc gaacgaaacc agggacgagg
    85621 ttatcacgac tctcttctct catccggccg atgctgccgt gccaaaggtg aagggttaca
    85681 aggtgatcgt ccttccccca gacgaggaag gcctgcctga cctggaggct ttcaggagcg
    85741 ttgtctccaa tagaactgcg gcgatctttt tcaccaaccc cgaggatacg ggcatcttca
    85801 acgccaggat cagggagttc acagatctgg cccatcaaca cggagccctt tgctgctatg
    85861 accaggcaaa tgcgaacggc ctccttggga tagcgagggc aagggaagcc ggttttgaca
    85921 tgtctttttt caaccttcac aagactttct cctctcctca tgggtgcggt ggccccgcaa
    85981 cgggcctgat aatggtagac gaaaaacttg cttgccatct gcctgtgccg gtggtcaaaa
    86041 aagacgagaa cagggggctc ttttacctgg actggaatct tccgaaaagt tgcgggaaaa
    86101 ttaaggattt cttcggagta gctcccgttg ttgtcagggc ctatgcctgg ataatgagtc
    86161 tgggtgcaga aggtcttgaa gaagcctcga ggaccgctgt gctgaacaat aattacttca
    86221 tgaaaaaaat gctcgacata agaggatgtt ccctgagtta cccgaatcat ccttcaaggg
    86281 tggaacaggc tcgctacagc tggggaaaaa tgaaggagga gacgggtttc ggaaccaacg
    86341 atgtgcagag gcggatcgct gatttcggta ctcactactg ggggagtcac gagccctgga
    86401 tagttccgga gcctttcacc atcgagccaa gcgaatccta ctcgaagagc gatatggatg
    86461 aatacctgga gataatggcc aggatttcca gggagtgcta cgaagagccc gaggtaattg
    86521 ctcatgctcc ccataacagt accatccacc atatagacca cgattatctt gatgaccctg
    86581 acaagtgggc tctgacgtgg agggaataca acaaaaagta cgaaggttat ttcaggcgga
    86641 aatgagtgag gccgtggcag aaaatatcag acgccttggc ctgatagtca atcccgttgc
    86701 agggatgggg ggatccgtcg ggctgaaggg gaccgatgga ttggatattc tcgaaagagc
    86761 cattgcgcta ggggccaaac ccggggcgaa ccggagagcc atggaagctc ttgaaaggct
    86821 ttgtgattac cggaagcaat tggagatagt gacatttccg ggcgagatgg gggccaccgt
    86881 atcaaatagg gcgggtttca ggcattcagt tttgaggggg acaagccgcc ttgaaaggac
    86941 gtcatcagaa gacacagtca aggcggcagg gcttttgatg tccggcggtg ttgatattat
    87001 tctgttcgcc gggggtgacg ggacggcgcg ggatatagta tcggcagtag gaacaggtat
    87061 accggtgata ggaataccgg ccggtgtcaa gatacactcc cctgtttttg caaggaatcc
    87121 ttcagcagcc ggagaactgg ccaggctttt cctgtttggt cccccgacag ggcttaggga
    87181 actggaggtt atggatatcg acgaagaatc cttcaggcag ggtcgtgtca gtacgtccct
    87241 gtttggatat ctcatggttc cctacgagaa gagccatctg caggggggga aatctggaag
    87301 cccaaggagc gaggcggtgg aagttgacgg cgcggcaagg gaggcagttt cgcggatgct
    87361 tcccggggtt ctgtacctgg taggtcccgg atcgaccact gcctccctta tggggatcat
    87421 gggctgtcca ttctccctcc ttggcgttga tgctgtcagg gatggaaaga ttctggggaa
    87481 ggatctttca gaaagcgaga tcctggaaat gatcgagccc ggcaatacac gtatcatcat
    87541 cacagtaata ggtggacagg gttttatctt cgggagaggg aatcagcaga tcggcccggg
    87601 ggtaataagg atggttggca agaaaaacat tacagttgtt gcggctcctt ccaagctccg
    87661 ttcccttccg ggaaggtcca ttttcgttga tacgggcgat acccagcttg acgaggatct
    87721 ttgcggctac atgagggtca ttaccggtcc ctcagaggag acccttgtga gcgtcagaag
    87781 ataacaaaaa agtcgcggcg ggcatgaacc aggcccgccg ctttcaaccg ggagctaaag
    87841 agcgcggccc ctttcttcgg cttttttcac ccagtctcct ttcaggatat cgcccttctc
    87901 gtggaccccg caggcaagca gtggttctgt ccgttgaatg cccagaagat cacatgaggt
    87961 tctgaaggag aaaagcaggc cttcgaaggc atcggtcctg tcgtcgccag ccgtggctac
    88021 gagcagcgcc tctttgcccc tcaggtccca ggcagcgttt tcatgaagaa agggaaggaa
    88081 tcggtcccag acaggtttta tctggctgct ccacgtatac cagtaaagag gagttgagaa
    88141 aacgatgacg tctgcctgcc tgatcctgtc gtgaataccg ttcatgtcgt cgacgatgat
    88201 gcagggattg cccctggacc agcattttct gcagtcgatg catcccccga gagtcattcc
    88261 gttaagtctg atctccgata tttcgttccc cttagacccg gctccacgaa ggaccgcttg
    88321 cgtgagggtc tcgctgtttc ctccaggcct ggggcttccc aagagtgcaa gaatgcgttt
    88381 catgcgtatt cctcctcata aaattgccga aatttgtcga atatcccggc tatttccctt
    88441 ttggtagttt catccgcatc gagaaggggc ggccttaccg gcccgccgta cagcccggcg
    88501 tggtccattg ccgacttgag gcccggaacc ccgaatctcg ccgtcacagc ctgattgatc
    88561 ccgatcattg acagttgtat ccttctggcc ctgtcgagat tgcccttttt gaaggaagcg
    88621 aaaatatccc tgcatgggcc ggcgaaaaga ttggcgactc ccatggtccc gccggaacat
    88681 cctactgcca gggatggaag aaaataactg cccgaacccg caaagacacc gaatccttcc
    88741 ggcgcgtccc tgcagatggt tgctatctgg acaatatcgg cggaactgtc cttgatacct
    88801 attatgttac cgtgagtcgc aatttttgcc acaagtgaag ctccgaggtt gaccccggta
    88861 tttgaaggca tgttgtagag gacaagagga gcgggtgaag aatccgcgat ttcaaaaaag
    88921 aatttctcga ggctttccgt agagttctgc cccttgtaat aatgtggggg aagcaggaga
    88981 agggcgtcta ctcccgcacg ggaagcctcg atggcaagtt ccgcggtttc cctggtggat
    89041 gggcagtgga cacctgctat taccttgatc ttccctttgg ccagtgctgc ggaagcaccg
    89101 aaaagatcga ctctttcgcg gaaagagaga agggggcatt ccccattgga tccgctcacc
    89161 acaatcccgt caagaggaag gttcatccat ttgcgaaagt taaactccag gtttttatgg
    89221 tctacgttgc cggatcggtc gaatggtgtc ggaacaggcg cgtagatgcc ttccagtttc
    89281 attcgggcac accccttcca gagtaaaatg atatatgggg acttcttctc cctacatcat
    89341 attatagatt agaatcagta tgggatgttt ctcgggtccc cggaggctct ggcatgaagg
    89401 aattccggat cggcacatgc tcctggacag acaggtctct tctttcgagc ggatggtatc
    89461 ctgactctgc aagggatgcc cagtcaaggc tgagacacta ctcgggattt ttcgataccg
    89521 tggaggtcga cagcacgttt tatgctattc ccgacgagag ctccttttat cattgggcct
    89581 ccaggacgtc cccgggtttc ctgttcaatg tgaaagcctt cggtcttttt acatttcata
    89641 ctcttccggt ttcgtccctg ccagcccaga tcagaccgaa gaatgcgtca cagggatcga
    89701 agatcaggat cggcgatatt accagagatc aaagggtcgg gctttggaat gcctttgttt
    89761 ccagggttct ggtccttcac cgtatggaac gtctcgggta tattcttttc cagttccccc
    89821 catgggttac cttttcggaa aagaatctcc gatggttcag aagaataggt gatcttgcgg
    89881 ctcccatgcg cgtggcactg gaggtcaggc acaggtcgtg ggtcgaaaat gaaaacaggg
    89941 aaaggcttct tgaggtcctg agggaggaga acatggccct ggcggccgtt gatgaaccga
    90001 agcttgactg gaccgtccct ttcgattggt ctatcacggc tacctggggt actgtcctga
    90061 gatttcacgg aagaaacgct cccgcctgga gcaaaaggaa tgcttccgta gcggaaaggt
    90121 tcaattacct ttacgatacc ggcgagcttg aggcattccg ggaggcttcg gagcgattgc
    90181 ctgacgatac gggcagggtc ttcctgatgt tcaacaactg ttttcgcgat aatgccgtca
    90241 gaaatgcgct ccagatgaga aagatcatgg ggttcggggc gattgttccc cccggacagg
    90301 aatcactggg actgggagaa gttgcaccgg acgaaaacga ataagagctg gctgaccatg
    90361 tttattttcg gggagggatg atctggatga gaatagtgtt ttcccccgtt accttccaga
    90421 aacttgagtc cgtcatcggc aggatgtttg cggggcacga ggtgataatt tccgaccttg
    90481 cagatctgcc ggaaaagatc gctggcgccg aggttcttgt aacggggccc cttgttgtga
    90541 gagatgatct tctggagagg tctccttcgt tgcgcctggt gcaccagtgg ggggtgggtg
    90601 tcgaaaaaat agacctcgat gcatgttccg caagggggat ccgggtatgc aatgttccct
    90661 ccatgggcac cgggaacgcg gaaggggttg ccgaaatagc cctgatgcat atgctgctcc
    90721 tttccagacg gttcgaccga tcacgggaga gcctggcgaa tcgaaggctt ttctcgcccc
    90781 agggagtatc tctttggaaa aaaagagcct gtgtggtcgg gctgggagat gtcgggaaaa
    90841 gtattgccgg gcggctttcc tgtatgggta tgaagatcac cggagtcaac aggagtcaca
    90901 gggaagacct ggaacgatgg gggatcgaca ggctttacaa acctggtgag gtggcttacg
    90961 ccttgccggg atgccgtttc gtgatactgg ccatggaact cagcgatgaa acgagggaga
    91021 tagttgacgg agggtttctg agagccatgg ataacgacgc atatctgatc aatgtcgcac
    91081 gggctgaact ggtcgcaagg gaggctctcg aaaccgctct tcggaagggg gacattgccg
    91141 gtgcgggcct ggatgttttc tggaacgagc ccgcagatcc cgatgaccct ctccttaata
    91201 accctcttgt aaccatcacg ccccacattg gaggagttac ggatgaggcg ataccgggag
    91261 tcgcctcgta cgtggcagag aacgttaccc ggcttcaaag aggggtatct ctgttgaact
    91321 gcctgaactg ctctgccgga gaaaaggaag gttgaacccg atgaaaaaag ccgttctttt
    91381 tgatttcgac atgaccctta tcgacagcag ccacggagtt actttctgtc tgaacatggt
    91441 agccgagagg ttcggccttg aaaaggtaga aagaccggag gtcctgaaga caatagggta
    91501 ccccatggaa gaagccatgg agatgctctg gggcgcattc gatccttcat ggacgaatca
    91561 ctacagggat tacctcgtgc ctttcgagta cgagagactt gctccctttg ccggagtaag
    91621 agaaacactg gaaaaactgc gagctaatga tgtcgtcatg gcggtagttt ccaaccgccg
    91681 caggcttttg cctgcactgg caggagcggg gcttgaggat tttttctcca tggctgtagg
    91741 catggaagat gtaagtgccc caaaacctga cccggaggca attaatctgg tgctggatag
    91801 acttgacgtg tcgcgagagc gcgcgttttt tgtaggagat tcagagatcg atgctatcgc
    91861 ggctcgtaac gccggtgtca ggtttatcgg aataacaacc ggaggaaggt cggcatcagt
    91921 tcttctggaa gagggggcct tcgctgtact ggaggatatg tccaaaatac tccgggtcgt
    91981 atgtccgggt ttttgtgatg tgcctcttgc ccaagaccgg ggagtgttat gattctggta
    92041 tgaagcaaac aaagttagtc aggacttttt actatttttt tgcggcatgc ataattcttg
    92101 gtgccctgct tgtctacaag gcatggtggg acaggtattt tcgcctgagg cccgaactgg
    92161 ccacagccga gcccgcggtt ctgtctattg atgttcctgt ggacgttgcc cttctgtggg
    92221 aggaatcgat aatagtctcc ccttccacgg gaaagatatc ctttcccgtc gggagggggc
    92281 ccttgtattg cccgaagggg gaaaccatag cggtaatatc cggttcttcc ggtaaaaaac
    92341 agatcagaat gcccgggccc ggttatttta tggcggccct tgacggacag gaggggaaat
    92401 ggtcatacgg ggatctgtgg ccgggcagca acaggatccc ttcgatccag aaaccggttt
    92461 tcatcgccga aggtgtggcg gtccttcagg gagaaccgat cggcaagttt gttccccagc
    92521 ctcgacagtt gagatgcgtc gcctatatcg acagcgcgac cgtggccgaa aacttttcgg
    92581 ccatggaata tctggagata aaaagagatc ccgccgactt gcccttcagg gcagagataa
    92641 gggctgcaag ggatcttggt ccggccctca aggtttacct gactttgcct tttttccccg
    92701 gggagatact tctttccagg acaatgtcat ttttgcatca cacgggccag agcagcggtg
    92761 tgatgatccc ggagagttcc gtaactctaa ggggtggtca aaaggttgtt ttcctggtta
    92821 cgggggacag gtcaagagcg gtaaaggtcg agggaatacc ggtgtcggga aagcgttttc
    92881 ttgttgagtc aggactctcg cccggcgatc ttgttgtcgt gtccggctca gaggccgagg
    92941 agggagagat cagaatatgg tgagcgctat ctcgtgcaat atccagaaca tccgcgagag
    93001 gatcctgaag gctgcattgg cagcaggccg aaacggggac gacgtcagcc tggtggctgt
    93061 cacaaaaacc cgttcggtcc gggagatgat cgaggcggca agttgcgggg tagaggccct
    93121 tgccgagaac cgtgtccagg aggcactttc aaaacatgag gaatggccgc ccgaactgga
    93181 tatttcgtgg catctggtgg gtcatctgca gaagaacaag gccaggaagg ctgcggctat
    93241 ctttgactat attcacagcg tggattcaat ccagttggcg aatactctgg aagccattct
    93301 ccaacctgag ggcaatattg tccggatcct cgtcgaggtt aatgttggtt ttgatccaaa
    93361 caagaacggt gtgcttcccg gggaccttcc tttcctggtc gaggaaatcc tttctgattg
    93421 tcattccctg agcctgtgtg gattcatgac cgtactgccc ctgggaattg atgaaaaaat
    93481 gacccgtact ctctttgccc ggctcagaca gctgcgcgaa gaaatggtat cccgcttcgg
    93541 aatccccttc ccggaactgt ccatggggat gagcggggac ttcgagtacg ctgtgatgga
    93601 aggcagtacc atggtaagga taggaagcgc gatatttgga gagaggcaaa ggtagcgaaa
    93661 aaggcatttt tgaaaggagg atttttgaca gtgtccgatc ttcttacggt tatcgatatc
    93721 gagaacatca acttcagaaa gtcaatgagg ggctacgatc cggacgaagt cgaggagttc
    93781 ctggaaaagg tagcgggaag cttgcagatt tattcagaga aaatcaagaa cctggaagtc
    93841 caggtatccc gtttcgatga ccagatgaag gagtatgtgg aacttaaacg ttccctccag
    93901 gacgcccttg ttctcgccca gaagagcgcg gaagagcggg tcaacagcgc cgaaagccgg
    93961 gcggaagtga taatagccga ggccgaggcg caggcccaca agatagtgat aggtgccaag
    94021 gacagggtcg atgagcacaa gagggagatc gagagactca gacagatccg ttaccagttc
    94081 gtggctgagt tcaagggact tttagccaaa tttggatcta tgctgaatgt ccttgaggct
    94141 cctcccgagg atcgtgaaca ggaacgtgcc ccggaaagat ccagggaaag agaaagatac
    94201 ggcgagcact ccagcaaaag cgattgtgag gaaggacagg aaacagtcgc cgagcccggc
    94261 ggttctggtg atgtttccgg tctggaaact gccaggtatg gagaggagcg ttcatgacgg
    94321 aatccctcgc atgggattct catgtaaggt tccttaaggg tgtcggaccc gccagggctt
    94381 cacgtctggg caggctgggg gtagatacgg ttgaggacct tctgtatttt ttccccaggc
    94441 gttacgagga caggcgaagc atcatcgatc tttcgcgcct tgaggtcgga tccgtctccc
    94501 acgctatagc gtcggtggta tccatagaga gaaggccgac cagaaaaaag aacctttcca
    94561 tagtgaatgc cctgctgagc gacgggagca gcatggccca ggcggtatgg ttcaacagga
    94621 aggggcttga aagggtcctg atccccggaa cgagggcctc cttttacggg agggtggagt
    94681 ccaggggcgg ggtggtccag atcgtgaacc ccgagttcga gatactggaa gataaggatg
    94741 cagatactcc gggccttttt gcgggaatag tacctgtgta ccccggaacc gaggggcttt
    94801 tccacagatg ggtcagatcg cttgttttta atgcagtgga gaccttcgcc gcaaacctcc
    94861 ccgatccttt acctcctgaa attcgcgaga agaacaggct tgtttccatc gaagaagccg
    94921 tccgttcgat gcacttcccc gaaggaaggg agtcatggtc cgcagccaga aagaggctgg
    94981 ccttcgacga actgttcctt ctccagacag ctctcgcgct caggaaaaga agctacgagg
    95041 aagagactgg cgggtatccc ctgtcctggg acgggccgat actccaaaaa ttccgaaaag
    95101 agatattgcc ctttgaactt acgtcggagc aggaaaaggt ccttggagag ataacccgcg
    95161 acagcaggcg caatgcgccc atgaacaggc ttcttcaggg ggatgtggga tccgggaaaa
    95221 ccgtggttgc tcttatgttc cttctggccg ctgtcgacag cggcttccag ggggcgataa
    95281 tggctcctac cgaagtactt gccagacagc atcaccggaa gatatcaaat tggctttctg
    95341 gtaccggtgt cgaggttttg tacctttcgg gttccatgac cagctcgcaa aagacggaag
    95401 cccttgccca tgtgatgtca ggcagaccgg gcatagcagt tgggacccat gcccttatcc
    95461 agaagggagt ccgtttcgga agactgggag ccgttgtcgt agatgaacag caccggttcg
    95521 gtgttctgca gaggagtgcc ctggcggaaa agggccagtg cccgcatgta ctggtaatga
    95581 cggcgactcc cataccaaga accctgacgt tgtccgtata tggagatctt tcattttcga
    95641 ccatagagga acttcctccc ggaaggaccc ctccggtgac aaagcagata tcaaacgccg
    95701 gggaccctcg cctgatccgt ttcatggaag aggagatatc agggggagga gccatctact
    95761 gggtatgccc ggtgatagag gagaacggtt ctctgccact aacgccggtc atcgggaggc
    95821 acaggatcct ttcggagata ttcggtcagg agagggtggg gatcctgcat ggacaggtac
    95881 cttctggtga aagagaaagg atcatggatc ttttttctac ggggaaactg tccgttctgg
    95941 tttcaactac cgtagtagag gttggtcttg atgtgcccga agctacggtg atgatcatcg
    96001 aagacgctca ccggttcggt ttatcccagt tacaccagct gcgggggcgg gtaggccgtt
    96061 cctccagacg cagttactgc ttcctggtgg gcaaccctaa aacaccggaa gggatagcaa
    96121 ggctgaaggc catgtgttcg actgcagatg gcttccggat agcagaagcg gatctgtctc
    96181 tcagaggccc gggtgaactt tgcggcatca gacagcatgg agtgaccgat ttcaaggttg
    96241 ctgacctctc cagggacagg gtccttctgg atgtctgcag ggaggatgcc atcaatctgg
    96301 tctccaaaga ccccctgctt gatggatacc cggtactccg gaaggaagtc ttcagaaaac
    96361 tgggtcgcaa gctcaaactg gtggaaacgg cgtgatctct catggatatg gaaaaagatg
    96421 acaaactgat gagttatgcc gttaggctga cttcccggtc ggaatatacg tgtgcccagg
    96481 taaggaggaa gctgcttgcc aggggagctc cagagttaca ggccaaagaa atatgctccg
    96541 aactggaaag ttgcggcctt gtgaatgatg tccgttattg cgagctcttc gtctccactc
    96601 atcccgaatt tggattttca agattgaaga tggagctgtc aaaaagggga gtggagaggg
    96661 ctgttattga atcgaccatc ttaatggacg aggatgatga gatctcgcgg gccgttctgc
    96721 ttgcaaggga gtgggcggat ttcaccgaac ccagaaaaat cgcggcacga ctttccagaa
    96781 gaggtttctc cgcaagggtt atcacttcag ccatagaacg ggcttgtgat acttcctcct
    96841 agtctgtaga atactttcgt atatcttttt cgaaaatagc gactgccgaa taaatcgaca
    96901 ggtcacgaaa cttgacaacg gaataaggag tggtgttcac atgttgcccg taatgctagc
    96961 agcagctttt gccgtcggca ttccgatcgg cttccttgcc tacaagttta tgagcgacaa
    97021 gaagctcaag ggaacgaggg agcaatcgga tcaacttcta aaggatgctt ttcaggaggc
    97081 cgacaggcga aaaagagaga tgctcacaga agcgcgtgag gaaattcatc gcctcaggca
    97141 ggacgcagaa cgtgagatca aggatcgccg cgcggaactt cagcgcgcgg agcggagact
    97201 cgaacagaaa gaggaaaacc tcgatgccaa gatggatgcc ataagccaga gggaggaaga
    97261 cttccgtaaa cgccaggagg atctttccgc gaggctggag gagatccaat ccatccggca
    97321 gaaacaggtc gaaaggctcg aggagatagc gaggatgtcc agggaacagg caaaggatgt
    97381 ccttctgcag gaagtggaac aggaagcggc ccacacgata ggtttaaggc tgaaagaact
    97441 tgaggaaaag gcaaaaaggg aatcggaaag aaaatccagg gagatcgtgg taagcgcgat
    97501 acagagatgt gccgtagacc atacttccga aatgtctgta agcgtggtct cgctgccctc
    97561 agatgaaatg aaggggcgca taatcggcag ggaaggcagg aacatcaggg ctttcgagac
    97621 attcacggga gtcgacctga tcgttgatga cacacctgag gctgtaacgc tgagcagttt
    97681 tgatccggtc cgcagggaag ttgcacgcct ttcacttgag cgccttgttc tggatggaag
    97741 gatacacccc gcaaggatcg aggagatcgt ggaaaaggct cagaaagatg tagaggagaa
    97801 gatactggaa tcaggcgaag aagcccttct tgagacgggc atcaagtcca tgcacccgga
    97861 actcgtgaaa ataattgggc aactccgtta cagatcaagt tatggacaga acgctctgtc
    97921 ccatagcctt gaggtttcgc atcttgccgg gataatggcc gctgaacttg gcctggacga
    97981 acagatggcc agaagagccg gtctccttca cgacataggc aaggctgtgg atcaccaggt
    98041 agagggtccc catgcactta ttggcaggga cattgccaaa cgtttcgggg agatcccgga
    98101 ggtcgtcaac tcgatcgcgg ctcaccatga agatgtggag atgacttctg tgtatgacgt
    98161 tcttgtctcg tctgccgacg ctatcagtgc ttccaggccg ggggcaaggc gcgagagcct
    98221 cgaatcctac atcaagaggc tggaaaaact tgaaagtctt gccaagacct tcaagggagt
    98281 gaccaaggcc tatgccatac aggcaggacg tgaggttcgg gtcatggttg cgccaaacag
    98341 cctggatgat ggcgctatcc ataaactggc ctacgatatg gcaaggaaga tcgagggaga
    98401 gatgaaatat ccaggtcaga tcaaggttat tgtgatcagg gaaacacgat cagtggaatt
    98461 tgccaaatag gaaaaaagtt catgagaata ctctttgtgg gtgatattgt cgggaaaccg
    98521 ggaagaaagg ttttttcatc ggttcttccc ggagccagac aaagcgatgg ccccttcgat
    98581 tttgtggtcg tcaatgcgga aaacgcggcc ggcgggtttg gacttacagc aaagataatg
    98641 caggaacttt tcgatatcgg ggcggacatc cttacaagcg ggaaccatat ctgggacaac
    98701 agggatgccc tgcctctcct tgacgaggag agccggcttt taagacccgc aaattaccct
    98761 ccagaggcaa ggggaagggg aatggccacg tttgagaaaa aaggcctgaa actggcagtt
    98821 ctcaacctgc agggccgtat cttcatgcag gccctggatt gccctttcag gagggccgac
    98881 gaggaactga cgtcccttga ggccgggatt cccgtacttg ttgatgttca tgcagaggcc
    98941 acgtcagaaa agagagccct ggctctctat ctggatggga gagtctctgc ggtcgtgggg
    99001 acccataccc atgtgcagac cgccgatgag gaaatactgg agggcgggac agcgttcatc
    99061 tcggatgtag ggatgaccgg tggtcacgga ggaataatcg gaatggagag cgaatccatc
    99121 atttcgaggt tcctgaccgg aatgccatcg aaattcgtag tatgcgggaa agattcaaga
    99181 atggatgcag tggtcataga tatcgacgaa aaaaccggga aaagtatctc tatcaaaagg
    99241 ttgtcactgg attcctgaaa agaaactttt tgaacggaac atctccccca aaaacgagcg
    99301 gcccccttca acggggtgcc gctctttttt tatcctgtca cccggagaat ctcccgaaag
    99361 ggtccatcta acagatccgg ggcagttcct cccccacagg tttatccacc agcctggtcc
    99421 cgccggataa agtgtggatc ccgacgtatc ctggatgcgc ttccgtgaca gatccaatca
    99481 gggatgctct tttccccagc gggttatttc tggcagctgc caggacttct tcggttttct
    99541 ccgcggagac gaccagcgct gcgcacccct ctccagccat gtgcagaggg tcaagaccca
    99601 ggagatcgca caccgacagg gatgcctcgt cccagggaat gagagtctcg tctatttcga
    99661 taccgacccc tgcggattcg gcccattcgc acatgaccgt ccccaggccc ccgcgagtac
    99721 agtccctcat gcacctgatc ccctctatgg ggagcatttc ctcgatcatt ggccaaaggg
    99781 cggcgcagtc cgagaacagc ccatggacgt tcaggccgta tcttacggcc gcgatggtgg
    99841 ctccatgcat cccagggggg gaggtaatga ttatcgaatc ccccggagat atgttcgccg
    99901 taccgagttt tgaagaggaa cgagtcttcc ctaccgcaca tgtggtaagg aacaggccgt
    99961 ccgcctggcc tttcggaacg accttggtgt cccctgtgac aagttctatg ccgatcattt
   100021 cacagatctc cccggcgctt tccatgtagc gggaaaggct tgaaagaggg aagccctcct
   100081 cggctatgac cgcgagagca atataaagag gttttgcccc tcttacggcc agatcgttgg
   100141 cgctgccgca tatgcagaga cttcctatgt tgccgcccgc aaaatcggga ggggttactg
   100201 tgtatccgtc aattgtcatc gcgaggcctc cgggcagaag ggcgcaatcc tctctgtcgg
   100261 cgacgggtcg gcctggaaaa ttcccgagaa tggttgaaac caggtcttcc gtcagacgcc
   100321 ctcccgcacc gtgtcctatg ctgatcgttt ccatgggtca tctcctccgg taaaaaacct
   100381 gtaatgggct gaacaactgc cctctcccga aaccatgcac ggtcccacgg gatgcgaggg
   100441 agtgcaggtc cttccgaaaa gggggcattc attcggtcca atcagcccca tcaggacctc
   100501 cccgcaggag cagccctgcg ggggtggagt cctggatatc ttcaatccaa gccgatttag
   100561 cgcgtcaagg ctggaaaaat cttccctgaa agcgtaaccg gaaagaggta tttcccccag
   100621 gcctctccat gatgtgtccc ggaggcagaa aactttctcg aggagtttct gcgcaacagg
   100681 gtttccataa tttcttgcag cctgtccgta catttgcgcc acacgaggcc tgtcttctct
   100741 tacctgcctg gctatctcca cgagtccaag catgatatcc tcagccccaa aacccgctac
   100801 cgagcaggct ataccgtatt ttgcgggaag gaactggtaa ggatgaagac caattaccac
   100861 gctgacatgc cccggcagca gcagggcctc gagcctgata cggctgtccg aggcaagtgc
   100921 ctcgaggaca gggggaacgg ccttgtgaag gttgagaacg gcgaagttcc gtatgtcatt
   100981 tttttgggca tccagcacca ccgaggccgt tgcaggagca gtggtctcga acccgaccgc
   101041 taccagaacc accagtttct ccggggtcat tctggcaata tcaagcgcct gggctgccga
   101101 ggttatgatc cttacgtctt ttcctcgaga tcttgcgtcg agaagggatc ctccgcttcc
   101161 tggtactcgc agcatatccc cgtaggtggc agttatgact ccatcaagtg aggcgagaga
   101221 tatggctgca tctatctctc cctggtctgt gacgcagaca gggcaccccg gtcccgagac
   101281 cagttcaagg ttgggtggga aaagagatct cagcccctgc cggaatatag agacagtgtg
   101341 ggtcccgcag acctccatta cccttaaggg acggtcggca agaatatcca gtgccttcgt
   101401 taggagtgag accttatccc tggtttcact catctcattc gttggcaagg cggcgcactt
   101461 cctcccagag tgcctccact tcgcggccat tctcctcgag gaggtgttca atagcaaaac
   101521 cggcatggac cagtaccaca tctcctacct ctacacccgg gacgaaatct gtccttatct
   101581 cggtctctac agcccccgcc gatgcaaggg ccctggtttc atcgatcagg ctcctgatcc
   101641 tgtgtggtat agcaagacac atggctacaa ctcccgaaaa acagtatgga caacggcaat
   101701 cattttatct cctacgtgtg tatgagcaag ccagcaatta aaaactcctt gccctggcat
   101761 cgggttgtgg ctaatcttgt catgggggta atggtcgatg gatcgtatca gggtcggagt
   101821 tctccaactc agtgttgtaa gagaagatgc cgcaaaaaac agcagatatg tcgggggaga
   101881 gatacgggat gcggcgaggg gcggatgcga tgtgatagtt ctgcccgaac tctggaacgc
   101941 ggcattccct ctccttggtc cgcatgttct ggtagacgaa aaatccggta ttgtagagga
   102001 gatagcgggc gcggcgttca ggacaggaac atggataatt gcgggaagta tggccatgag
   102061 aactcccctg gggccaagga acagatgcct ggttttttct cctgaaggta atatcttttt
   102121 cagatacgac aagattcacc tttatccagg cctgagggag cctaacattc tctcacccgg
   102181 atcaaaactc gggttttttg atctggatgg gatcccctgc ggagtaatga tctgttttga
   102241 tgccgagttc ccggaggtgg catctgcact atcgcgaaag ggtgccagga tacttttcgt
   102301 cccgggagcg tggaagagtg agtacatcag gttatggagg gcaatacttc tttcaagggc
   102361 tatagagaac caggtatttg tggttggagt gaacagatgc gacaggggga aggaaagctc
   102421 tttcgggggt catagtatgg tcatagatcc ttacggggat gtccttcttc accttgatga
   102481 ttctccaagg ttccagatgg tcaaccttga tctcggaaag acaatttcag cccgccggga
   102541 acacaaggtg atcgattcca gaaggcccgg agtatacagg cgatggaact gaattttttg
   102601 cccctttaac aaagtagggc ttttcagaat tgacttgcct gttgactctt tcacggcttg
   102661 taatataatg cccaaaattt gtatgctgtt tctgctggca gttatgagcg cctgactttt
   102721 cctggtagaa caattgccaa aaaaagggga cggccttata atgacaagaa aatgatgaag
   102781 aacacaaggg gggtgatccg gggattattt tagccagcaa agaaacggca acagttttcc
   102841 tgttcattct cagacccgta tcaagaggag ggatatcatg agcaacggaa cagaacgcga
   102901 acagtggggt tcgaaactcg gttttgtcct cgcggccgca gggtcggccg tagggctcgg
   102961 taacatctgg cgttttcctt acattactgg acagaatggc ggagcggcat ttgtgcttct
   103021 ttacctggcg ctggtcttcg tgatcggatt ctccgtaatg cttgcggaga tagctatcgg
   103081 tcgtaacgcc cagctcaacg ccgtaggctc cttcaggaag atcaagggcg ggatttggcc
   103141 actggtcgga tggatgggtg tcatcgccgg cttcatcatc ctgtcctact acggcgtcat
   103201 cgccggatgg acgatggcat acatcctcaa atccttcacg ggccttatgg gtgccgctgc
   103261 tgaaggtgca gccggtgacg tttttggagg cttcatcacc aacaccgccc aggccgtagc
   103321 ctggcaggcc ctgttcatgg tcatcaccat ctacattgtc tacaagggca tcggcggagg
   103381 tatcgagaag tactgcaaag tcctcatgcc tgcactcttc ctgctgctcc ttgtcctgat
   103441 cgtccgttcg gtcacgcttc cgggagctgg cgagggcgtc agtttctacc tcaagcccga
   103501 cttcagcaaa ctgacagccg gagccatggg cagtgccctg ggccaggcct tcttctcact
   103561 ctcccttggc atgggctgca tgatcaccta tggaagttac ctcggcaaga aggagatgct
   103621 tcccggcgcg gcgatccagg tctgcttcct cgattccctc gtggcgttcc ttgccgggct
   103681 tgtcattttc cccgcggtgt ttgcctttgg tcttgatgcc ggagcaggcc cgggcctcac
   103741 gttcatcact cttcccaacg tatttgccca gatgcctggt ggagcggtct ggtcagccat
   103801 gttcttcctt ctcctttttg ttgctgctct cacatcggca atatccctgc ttgaggttgt
   103861 tgccgcctac tttatcgacg agatgaagtg gacacgctcc aaggccgcgt gggtcatggg
   103921 cggggtcatc ttccttctgg gtattccttc gacgcttggt atctccggag tcatccccaa
   103981 actcgccgga agggatttcc ttgattcagc ggacttcctc gcatccaacg tgttgcttcc
   104041 cctcggcgga ttctttattg cccttttcgt gggctggatc tggaccgatg gagccaagca
   104101 ggaggtcgag aacggcggag ccgccccgtt tggactttac gtcccatggc tctgggtctg
   104161 ccgtgtcgtt gcgccccttg ccattgccta catctttatc acgggcctca agtggtaggg
   104221 taaatacagg accagaagcg ggggccatgg cccccgcttt ttttataagc gttgaccttt
   104281 gttcgctatc atgagaaaat aaccaaagag ttactggatg aaggctgtga gagatgctcc
   104341 ccgcaggtct gtcccagggc ggggggagcc gaaggggcaa gctcctaaag gggcgaaact
   104401 ctcaggcgaa aggatcgcag ctggacaagt ctctggacag gcgcgtagag gcgctggagg
   104461 ctccgggcaa gttcccgggt ctgctggaca gagaacccac ctatttgcaa aatagggggt
   104521 ttttcggtgt tccagaaaat cgagtgcctt acaaataacg ccgtactctg aaactgctcc
   104581 ccaaataccg ccttcgttga aggtgggcct ttgcgtgtgc taaaactggc cagagacagg
   104641 atccatgccg gttggcatct tcttacccac cccctttacg gaaacataca gccctttcag
   104701 cagccttaca ggtcccttct gatcggtcgg ccggatcctg aagaaaggtc cggacctgat
   104761 cccgagatgc tttgcgtgct cgaaaaagcg atactgcttt tttctcctcc cgaagaaggc
   104821 gggcggccac ctgcccgtca ttcctccgca atccttgaag attatgccga gcttgatgcc
   104881 tgccttttgc gtgaaagcct cgaaaaatat ggtatatgga agcccgcttc ccgatagagt
   104941 gatcattgct cctttttggg tggagagaag atgttcttga agcatgaacc gaagggaggt
   105001 gaaccgagtg atagaggtaa cgaaagataa ttttgaggag gaggttctca aaagcgacat
   105061 gccggtgctt gtcgatctct ggggaccaaa atgcggaccc tgtctggccc ttatgccgca
   105121 ggtcgaagaa ctgtcccacg agtttggcga caaggtcaag ttttgcaaac tcaacgtcgc
   105181 agaaaaccgc aaacttgtca tatctctcag ggtgatgggt gtgcccacct tcctgttcta
   105241 caaggggggc gagatagtcg acagattgag cggagcggaa gcagttcctg aagcgatcag
   105301 ggcaagggta gaaaagatga actgatcgcc ggccaaagtt cgaaaggggg gaaaccgaat
   105361 tggaactcaa gatccagaaa gtccttgtca aggccctttc ggaaggggac aagacctcct
   105421 tcaacgctgg aaccctgacg atcaacaggc aggagatcat cgatatgctg ctgaaggatc
   105481 agaggtttac gagcgtcaag gttgaacttg cgcgtccggg ggaaagcatc aggatcattc
   105541 cggtcaagga tgttgttgaa ccaagatgca aggtggaagg accgggtgaa gttttcccgg
   105601 gtttcatcgg cgatgtcgat accgtcggag aagggacgac ccttgtcctg agcggtgcag
   105661 ccgtggtgac atgtggaaag atagtagggt tccaggaggg tatcattgac atgtcgggac
   105721 ccggggccga gtataccccg ttctcgcata ccttcaatat cgtcctggtc gtcgagccca
   105781 cagaagggct tgagaaacat gattacgaat ccgcgtgcag acttgcgggt ctcaagacgg
   105841 cccattacct ggcttcaaag gtcaagggtt cggccagtgg cgaggtcgag accttcgcta
   105901 ttccggatct tgcaaaggca atgaagtccc atgaaggact gcccaaggtt gcgtatctct
   105961 acatgcttca gtcccagggg ctcctccatg atacctatgt ctatggtgtc gatgccaaga
   106021 agataatccc gaccttgatc catcccaacg aggtgatgga cggagcaata gtgtcgggca
   106081 actgcgtatc ggcctgcgac aagaacagta cctactccca ccagaacaac ccgatcatcg
   106141 atcacatgta caaaagacac gggaaggact tcaacttcgt cggatgcatc ataaccaacg
   106201 agaacacaac ccttgccgac aagaagcgca gctcttccta tgcggtcaaa cttgcgaaga
   106261 tgctgggggt tgacggcgta ataatcagcg aggaagggtt tggcaatccc gataccgacc
   106321 tgataatgaa ttgccgcaag gccgaacagg cgggcataaa gacggttctc gttactgatg
   106381 agtacgcggg aagggatggc gccagccagt cccttgcaga cgcgtgcccc gaggccgacg
   106441 ccgttgtgac cgcggctaac gcaaacgaaa cgatagtgct gcccaggatg gacaaggtaa
   106501 taggtcttcc ggacacatcc aacataatcg cgggcggttt cgacggctca ttgagggccg
   106561 atggctctat cgaggtagag atacaggcaa tcaccggagc tacaagcgaa cttggcttca
   106621 gtcgcatcgg tgcctatacc atctgatttt ttaaatgaag gaggtgcttg cagtgggcaa
   106681 acttgccgga aaaaaggtcc tccttctcgg cgaaagggac ggtgtagccg cccccgccat
   106741 ggaggagagc ctcaagaatt gcggcgcgga ttttgtattt tccgtaacgg agtgttttgt
   106801 ctgaaccgcc gcaggagcca tggatctgca gaaccagcag cgcgtcaaag acgcggctga
   106861 caagtacggg gcggagaatc tggtagtcat acttggatcc tccgatgccg aaggtgccga
   106921 aatttacgcg gaaacggtaa ccaatggcga tccgactttt gcaggtcccc tggcaggagc
   106981 cccgttgggg ctcgctgttt accatgtttt cgagaaggat attcgcgatg aagccgagcc
   107041 ttccctctgg gaagagcaga tcagcatgat ggaaatggta ctggatacag aagctctcgc
   107101 caacgccgtc aaaggcgtac gcgaacaata cgggaaatat accctctagt gggttacccc
   107161 gggaaagggg ggaacgaatt aatggcgtac aaggtagtcc actatatcaa ccagttcttc
   107221 gccgggatcg gcggggagga tatggcaggt cacaaaccgg aggcaagaaa gggttttgtg
   107281 ggtccgggac aggcccttgc cgcagctttt ggttccgagg cggaagtcgt cgggacggtc
   107341 atatgcggtg acacctattt tgcggacaac ctcgagaagg cggagaaaga ggtcatggac
   107401 ctcataaaaa ccttctctcc tgatgcggta gttacagggc ccgcgtttaa tgcgggaaga
   107461 tatggcaccg cctgcggatc cgtctgcgag gccgtaagga aaaacctggg aattcctacc
   107521 gtttcaggta tgtaccacga gaaccccgga gtggacatgt tccgcaaatc cacctacatc
   107581 gtcagtactc cggatagcgc cagggggatg aaggaagcca tacccgccat ggccgctatc
   107641 gctctcaaac ttctcaaggg cgaaaccctg ggcaagcccg aggatgaggg atatctcgaa
   107701 agaggcgtaa gggtcaactt tttcatggac aagaccggag ccgagagagc cgtcgacatg
   107761 ttcgtgaata aacttgccgg aaaacctttt gtaacagagt acccgatgcc tacttttgac
   107821 agggtggctc caaatcctcc gataacggat atgaaaaaag ccaaagtagc ccttgtcacg
   107881 tcgggcggaa tagtccccaa gggcaatccg gatcatatcg aggcatcgag cgcttcaaaa
   107941 tacggtgaat acagccttgc cggggtgatg aagatcgacg ccaccaccca ccagacggcc
   108001 cacgggggtt acgacccgac atatgccaac gacaacccga acagggtcct tcctctcgat
   108061 gtcatgcggg atctcgaaaa agagggagtc ttcgccaaat tccacgaaaa gttctacaca
   108121 accgtcggca atggcacctc agtcggaaac gcaaggcgtt ttggagctga aatcgcacag
   108181 aaactgaaag atgacaacgt tgatgccgta atagtcacat ccacctgagg cacctgcact
   108241 cgttgcggcg caacgatcgt aaaggaaata gaaagggcgg gttttcccgt cgcccatgtc
   108301 tgcaccatag tcccgatttc tctcacggta ggagccaacc ggataattcc ggccgtcgcg
   108361 atacctcatc ccctgggcga cccgtcaaag tccccggaag aggagaagaa gatcaggcgg
   108421 gctattgtcg agaaagctct gcgggctctt cagaccccgg tagaggacca gaccgttttc
   108481 aacgactagt tattcatgca gtggcccgga gatatatctt ccgggccact gcaacagaat
   108541 catagggggg atctgccaat ggctcacgta gcgatcaggg gttattccta ttgtctcaac
   108601 cacaccccgg agataggcct ctattatgga aatactccat acatggagaa ggtttcacac
   108661 ggagagacgg agttcataaa aaagctggcg ggtagcattc agtcctttga ggaagcgtgc
   108721 tgctatcccc cgaaccaggc tttcatcggt ggtatggatc tggacgaact atccgcgcgc
   108781 cttgccccga tgtttgagaa caggctcgaa aaaggatccc gtttcggcag attcggggag
   108841 atcatgccgg agaatgaatt catcggcctg atggatatct gtgatgtttt cgaccttatc
   108901 tggctggagg aatcctttgc ctcagatgtt gcaaaagccc tttctgtcca tccgcttttc
   108961 agcgaaaaac tcatggcaag gctggaaaag gggcattctg cagcggaggt ggaggaagaa
   109021 atatccaaac acgggggcct gcctctatgg tggaaaggaa aggcggttgg ctgctgcaga
   109081 aagggtcacg atacagacga atccctttcc gctcacgttc ttcttgaaaa tattgccagc
   109141 aaggctggat cggtgctggc gttgctgcat cttttgaata atacgggcaa ggttcccggt
   109201 gacattgatt atgttatcga atgctccgag gaagcggccg gggacatgaa ccagcgcggg
   109261 ggaggcaatt ttgccaaggc cgtggccgag atagctggat gcgtaaacgc ttctggtttt
   109321 gatgttcgca gtttctgcgc tggcccggtg aacgctgtca ttgccggcgc aacccaggtt
   109381 gccagcgggg ccaggcccaa cgtggttgtt cttgcagggg gagccgtgcc aaaacttttt
   109441 atgaatagcc gtgatcatgt gaaaaaagga atgccggcac tggaagattg cctgggcaac
   109501 ttcgccgtgc tgctggagcc cgatgacggt acgtgcccgg tgatgaggct tgatgccctt
   109561 gggaaacact ccgtcggtgc cggagcgtct ccgcaggcta taacgtctgc cctggttctc
   109621 gaaccgcttc agaagatagg gctctccttt gcggatgtgg acaagttcgc tgcagaactg
   109681 cagataccgg agataacagt tccggctgga gcaggggatg tcccggcggc gaacttcaag
   109741 atgatagccg cccttggagt gatgaagggg caactgaaaa aagaagaaat ggcttccttt
   109801 gtggagcaga aaggcatccc gggttttgcc catacccagg gacatattcc gtccggggtt
   109861 cccttcattg ggcatgcctg cgaagcgata aaagagggga aattatcaag ggtaatgatc
   109921 attggcaaag ggagcctctt ccttgcaagg ctgactaatc tttctgatgg agcctccttc
   109981 ctgatcgagc ccggcaaggg agagaaaccg ggagctatga ccagggaaga tctccgcgaa
   110041 atgcttcttg atgcgctcca ggatctttcg aaaggtctga cggggtgaac tcgccatgac
   110101 cacgggcgga gaaaccagaa agatcgtcgg ggaggtcctg aaggaaatag tagaatccgc
   110161 gaggagtggc ggcccactcg tacgtgtggg tctgatgggc tctgggagtg aacttggtcc
   110221 cgaagaactg gccagagggg ccctgatcgc gcagggatcc aggcctggtc tccgcgtggt
   110281 aatgatcgga cccagactcg aaggctggga tatgctcgat tggatagaga cagccgattg
   110341 cgaggaggat atctcccggg ccatggagac ggccctccaa aagggggcca tcagcgcggc
   110401 agtcgccctt cattaccctt ttccccttgg agtaaccact atcgggagag tgatgacccc
   110461 cgcaaggggg aaacccatgt tcgttgcctc cacgaccggg accagttcta cggaacgcat
   110521 cgagggtatg gtcaaaaacg cgatatacgg tgttgctgtc gcgaaggcct ccggaatcgc
   110581 agaaccttca gtggggatat tgaacataga tggagcgcaa ctggctttca gggctcttgg
   110641 aaggctgagg gacaacgggt ttccggtcac tttcgggagc agtgtccgaa aggacggtgg
   110701 ggccatcctc agggggaatg atatcctggc cggggctgtg gatgtctgtg ttaccgatac
   110761 cctgacgggg aacgttctga tgaagatgtt ttcggcattc accaccggag gcggctacga
   110821 atcgacaggt tgggggtatg gtccatcggt gggtaaaggc tggcccgtta tagtctcgat
   110881 aatctcaagg gcttccggtg ctccggttat cgccaatgcc ctgcagtatt cggccatggt
   110941 ggcagagggt tccctcccga aactggttga ggagcacatc gctctggccg aaaaggcggg
   111001 tcttgaggag atactgtcgg aacttagacc caccagacag gcagaagaaa aagttccgcc
   111061 tcctccaaaa gaacccacag aagaggaact tcacggtatc gatgtacttt cgatcgagga
   111121 tgcggtggtt gctctctgga aaaacggaat atatgccgaa tctgccatgg gctgcaccgg
   111181 tccggtagtc aaagtaccgg gaagatctct cgaagaagcc ggggagatcc ttcgaaaaga
   111241 aggtttccta tagagaagga gggaaaaggc aagggtcaat gaagtgcaat ttgtgctata
   111301 atcttatcgg tatatttaat aattgaccct ttgccattgt ctgggggtgg tctcaggtcg
   111361 agagagtcca gcaaccggtt ggtgaaacga gtcataccgc aacaagaatg aagagaaaag
   111421 aaggagggat ttgttgttat ggaagccttt atgaggctta acggtgtagt caacggaata
   111481 gtctggggtc ctccgatgct tgcactgcta gtcggaaccg gcatctatct ttctgtcatt
   111541 ctcggtttcc cccaggtcag atacttcggg ttcatgttca aggaagtcct cggtaagata
   111601 ggcaagaagg ctgaggggga agggaccatc tcctcattcg gagcccttgc agttgcgctt
   111661 gcttcgacaa taggttccgg aaacatcgcc ggagcggcca cggcccttca ccttggcggc
   111721 ccaggagccc tgttctggat gtgggtcact gctatcttcg gcatgaccac gaagatgacc
   111781 gaggtctcgc tggctgtcaa gttccgcgag aaggatgctg ccggaaactg gcgcggcggc
   111841 accatgtacg tcatggagaa ggctctcggg cagaaatggc ttgcctggct ctttgccttc
   111901 ttcacgactt tcgcggcctt cgggatcggt aatgccatcc aggccaactc caccgcccag
   111961 gcactggagc tcggtttcgg agttccttct tacgtatccg gtattgttat agccgtcctt
   112021 gttgctctcg tcatcatagg cgggctgaag aggatctctg atgttaccac ctatctggtt
   112081 cccttcatgg ccgtcttcta tgtcatcggc ggtattgcgg taatagtcgt tcatgccgac
   112141 cttatcccgc aggctataag caacgccgtc tactatgcct tcaatgaccc gatggcgatg
   112201 cccggagcaa ttgctggctg gtcgatcaag gcagccctca caagaggtgt ggcacgtggc
   112261 gtcttctcca acgaagccgg tctcggttct gccccgatgg tccatgcgac cgctgtcaca
   112321 gatcaccccg gacgccaggg tgtctacggc ctctttgagg tcttcatgga taccattgtg
   112381 atctgctcca tcacctgcat cggtatcctt accaccggta ccctcaccgc caggccggat
   112441 cttaccggag cccagctgac ccttacggcc ttccagaccg tcctgggcac tccgggagtt
   112501 atgatcctct cggcggggct tgccatgttc gccttctcga cgatacttgg ctggtactgg
   112561 tacggtgaga cgggtgcggt ctacatcttt ggtctcaaga ttgccccgat ctacaaggtg
   112621 ctctggattg ctgtctgtgt cgtcggcgcc tggggtggcg gtggccagtt cctcgccaac
   112681 atctgggacc tctccgacac gatgaatggt ctcatggcgg cgcctaacct cattgcgctt
   112741 ctctggctct ccaacgagat gcgcaagata gtcaaggatt tcgacgaaaa gagaaagaaa
   112801 ggaacgatca agtagtcccg tccctgcggg atctgatcac cattcttttt tactgggagg
   112861 gagcggagtc tttccgctcc ctccctcatt gaaaaggagg acgacaaatt ggcactgcgt
   112921 ccaacccgta tggaagtcaa ccttgcaaac cttgccttta actgcaggtc catatcatca
   112981 ttccttgatc ccggttgtaa tatgatcgcc gtagtcaagg cagatggata tggtgtcgga
   113041 gccatggccg ccgccaaaac gttcagggaa tgtggttgcc agcgattcgc cgtcgcaaca
   113101 cccgatgagg cagtagagtt aagggatgga ggcataacgg aaccgatact cgtcctgggc
   113161 ccctcaccga gggagtctgc tgaagagcta gtggcagggg atatcacggc cgcctgcacc
   113221 gatgtcgctt ttgccgaggc tatgagtcgc gcggcttctg cgaggggtaa aaccgcgagg
   113281 ctgcatttaa agatcgatac cggtatgggg cggataggtt ttctcccttc cgaggcccct
   113341 tcggcagtcg aaaaaattac cgttcttccc ggtctggagc tggaaggaat tttcacccac
   113401 tttgcgtgtg ccgatgaaag aaacgctgag tacaccagta tccagttctc ccgtttcgga
   113461 gagacccttg atcttctcag acccctcaac gcaagatttc ggatcaggca tgtctgcaat
   113521 agcgcgggga ccgtcaattt tcccgagatg catctcgacg ctgttcgtcc cgggcttatc
   113581 ctttacggga tgtggccatc tcctttctgc aaaaggagcg tggaacttaa accggtattc
   113641 gaggtcaaaa cttccgtagc cgtggttcgt gaactttctc cctgcagcgg tgtcggctat
   113701 ggcctgaaat atatgaccag ggggacggaa agggtggcta tcctccccat tggctacgca
   113761 gatggattcg ctcgccccct ctccatgaaa gcctcagttc ttgtcagggg aatgagggct
   113821 ccgctaattg gaagcatttg catggatcag accatggtcg atgtgactca tatcccggga
   113881 gtcgtaacag gggacgaagt cgtaatagtc ggtgatcagg gaaaggaacg catcactccg
   113941 gaggagaccg ccgctcacct cgggacaata aattacgaga ttcccagtct ctttaccaaa
   114001 agggtcccga gggtctacgt atagattttt ctgaaacatt gacggcggct cgtgccgccg
   114061 tttttttgtg tgcaggaagg ggttataaac gctaaaatgt aaagatcctg tcgccaagcc
   114121 gggatgtgaa tggaggagtt cagcatgaat gatcttaagt cggtcctgag ggggttcccc
   114181 tccgacaggt tgaacgacag gtctgtctcg atgattgaaa gcgcagcgag ggagtgccgc
   114241 tcgtgggctg tgacaatgac cacggcagca gggagtgggc atccggccgg gtccctttcg
   114301 agcatggaga tatatctcat ggtatacgga gcggcaaaca ttacacccga aaactgtgat
   114361 aatcccgaca gggaccatgt cgtcataagc cacggccata cctcccctgg agcttacgct
   114421 gctttagccc tttacggttt ttttgacccg ggcgaagcca tggcccattt tcgacaggca
   114481 gggagccctt ttcaggggca tgtggagcga gaggttcccg ggatagactg gggaagcggc
   114541 aacctcggcc aggggctcgc agccggggtt ggttttgctc tcgccgcaag ggccagggga
   114601 agggtagaca atgtctacgt gcttatgggg gacggtgaac aggtcaaggg gcaagttgca
   114661 gaagccagaa ggatagcctc ccatcatggc cttgacagga taaccgtcct gattgatctc
   114721 aacgagatgc agatatcagg tcgaaccgaa gatatcatga aggccgatat cagaggtctc
   114781 tgggagattg acggctggca tgtgatcgaa tgcgatggac acgaccccgc ggctttgtat
   114841 gcttcaatgc tggaggcaga aagaacccgg cttccgtccg tcattatttg ccggaccctt
   114901 atggggaaag gagtctcctt tatggagggt attccggatt accacggaaa ggccgctgcg
   114961 ggtgaccttt accggagggc catggaagaa cttgaggccc cggccgacat gctggataaa
   115021 gccctcgcga aaagaactac tggaccggtc ctgaagggaa ggaatattgt gacgtgtcct
   115081 cccttgctgg atacaggcac tcccgcgaat tgcgatccat cgaagatgac tgacaacaga
   115141 tccgccttcg gcaatgctct tgccgacgtg ggtatcagaa attacggtgt tgagggacga
   115201 accccgattc ttgtttttga ctgcgatctg gccggatcgg tcaaggtgga cggatttgcg
   115261 gggaaatgcc ccgactggtt tgtggaaacc gggatccagg agcatgccac ggctaccgtc
   115321 gcaggcgccg catccacggc gggggttgtt ggcctgtggg ccgattttgg tgtatttggc
   115381 cttgcggagg tttacaacca gcagaggctt aacgacataa acgggaccag tctgaaactg
   115441 gttctgaccc acgtgggact ggatgttggc gaggacggta aaacccacca gagtatcgac
   115501 tatatggggc tcctgcgtaa cacattcgga tggagactcc ttgtccccgc cgaccctaac
   115561 caggccgacc ggatgaccag gtgggctctg ggagagcctg gcaacatttg tcttgcgatg
   115621 ggtcgaagca agatccccat cattctggac gaaaatggca agcccttctt tggtggagat
   115681 tatgttttca ggtatggtgc tgtcgatgta ataagaagag gttccgatgc cgctattctg
   115741 gccgttggcc acatggtttc ccgcgccttg agagcctggg agatactgaa gagcagaggc
   115801 gtaaacgtgt cggtctacca tgtcggctgt ccccttcacc tcgaccggga tgctctttca
   115861 gaggcagctg gcaccggaaa ggtggtcact tttgaggatc accatgtggg ttccgggctg
   115921 gggtccctgg tggcctcgaa gtttgtggag acaggttgct gttgcgaact ccggaccctg
   115981 ggtgttaccg aatacgggga atccggcacc tcggcagatg tgttcacctc tatggggctg
   116041 aaccatgaag acctggtcga taccgttttg ggaatgaagg gcattaaata acggaggttt
   116101 tccgatggaa ctgatgaatc tgggcggacc cgtgatgtgg gtcattcttt tcctttctgt
   116161 tgccggggga gccatggcta ttgagaggct gtggttcacg atgaaggcaa ataccgatgc
   116221 ctctgctgtt gaactggccc tgggtgggca catttatcac ggcaggaagg aagaagcatc
   116281 gaaactggtc agggaaaagg aaagttccct tcaccggctg ttgcgggttg gtgtggatca
   116341 ctgggagacc gacccaggtg ctcttcaggt ccttctcgaa caggaagttc gaagggaggt
   116401 cttcaggtgg gagagaggtc tgggttttct ggcgaccctc gcaagggtgc ttccccttct
   116461 ggggctcctg gggactgtcc ttggaatgat agatgttttt cagaatcttc ccggaatgag
   116521 cggatccagt atgaccgtca tggccggtgg gatatggaag gcactgttga caaccgttgc
   116581 gggacttgga gcggccgttc cgcttatttt gtgtcatgcc tttctctcca accgggtaga
   116641 aagggcggag gagatgctct ggcgtggtgt tgatttcatg gtcagggaaa agatcctgag
   116701 gaacgggacc aatgaaaaaa ccggtatgga ataacaatat cctggttctc gggctatttt
   116761 tgtgttttct tgccgttgcc cctgtgagat cttccgcggc tttctccggg cgcatcgtat
   116821 ctcttgcccc ctctgtcacg gagtgtcttt tttctttggg gtccggggac ctggttgcag
   116881 gtgtcaccga tcatgacgtt tttcccgaag aggtaaagga acttccgtca gtgggagggt
   116941 atttcgaccc atcgctggag aagatcctct ctctctctcc tgacctggtc attggtattt
   117001 ccacttttca tgggcgactc cttgaaaggc ttgagtccat gggagtaaaa accctacccc
   117061 tgaccgtcca cagagggctg gagggggtag gtgattcgct acattccata ggagaaaaga
   117121 tcgggaaaag agccatggcg gaagatctct ggcgtgacat catggaaaag atcgatctcg
   117181 tcgcggaaga tgtcaagaga tcctttcccg ggggcccgcc atcgcttctg gtagtggtct
   117241 ggcacgaccc gctgacggtc gcgggagggg tcaactttat agacgacatc ctcacaagga
   117301 taggcattcc caatgctgcc ggggacataa actacacctt cccccagcta gacccggagg
   117361 gagttatcat ccgtgacccg gatatcattc ttgttccccg taccgaaagg ggcatgtctc
   117421 tttcccctga tgtcctggag aacgcactta agggacttcc cataagggcc gtggaggaag
   117481 gcaggatcgt atcggtttcg gcggatcttc ttttccaccc cggtccgagg gtagccgagg
   117541 cggcgatgct cctgctggga tcgataatgc agtttgaggg tttagtacaa tgaacaggat
   117601 gagaagaccg agggcgcatc ccgatgtaga actgaccccg ctgatcgatg tcctgttcat
   117661 gttgataatc ttctttgtcc ttacagcggt ctttaccgag tcgtccatcc ctgtgaatct
   117721 accagaggcc cagggggatc cttcgcggga aagaaccttc accctgacca ttgacgccga
   117781 aggcggcgtt tatgctgagg gtaagatcct gtccattgat cttgctgtga aaagagcgtt
   117841 tgaagcgtgg caggaaggca gaaaaattgt tatcgccgcc gaccgtggcg tggaatacgg
   117901 cgtggtggtg tccctgctgg accaacttag agggcaaggg gtggattcgg catcgctttt
   117961 ggtggaggcg acggaataac cgtggccagg tggatccctt ttttcgttct gagtgtcatg
   118021 atccatcttg tcgtaatact aataccgttc aattacggat caacagaact ggtggatccg
   118081 cgggtcatat ccgttgaaat cgagaccttt tcaaagcctt ccggtgaaac aggatctccc
   118141 gggagtgaaa ctgctcctct cccgaaaaag gttcctgtta agaccggagc tatcgaaaaa
   118201 caactgcaac cagaggccgc gttttcgagg tcggatcctg tcgtggccgg tgaaccatta
   118261 gatgatgaag aaccggaacc gaaaatcgtg gcccccgggg atggggtcaa gccttcggga
   118321 gggtccgcca cccctgttgt gaccgaggcc gacacgatca ggcgtgtaac gcctgtatac
   118381 cctcttgcga gcaggcgcag gggcgaggaa ggggaggtca ggcttcttgt cggggttgcc
   118441 agagacggaa gtacggcctc ggtggaggtt gtcggcacga gcgggttttc tgctctcgac
   118501 gaggccgcgg tcttcgcggt ccgaaggtgg cggttcgctc ccggatcgcc ggaaaaacta
   118561 atcgtacccg tcattttccg tcttgagtga caagaagagg tgtcgtgagc ctgtaaggcc
   118621 aactgggaac agggaaccct gacgggcccg gagttaagtc tttcccgaaa ggcaactcgc
   118681 ctgaaagcgt tcgtttctcc gcctgctacg gggcg
//
LOCUS       DAOTIQ010000002        78435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_261_length_78435_cov_7.640750, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000002 DAOTIQ010000000
VERSION     DAOTIQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 78435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78435
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_78435_cov_7.640750"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="P5541_00590"
     CDS             <1..1460
                     /locus_tag="P5541_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRV70502.1"
                     /translation="ETEYVESIKEDVAWLGFRWANLRFASDYFDEFYVWALELVRAGK
                     AYVDHQTAEEIRTNRGTLTEPGKDSPYRSRSVEENLELFGKMKDGKIPDGECVLRAKI
                     DMGHPNLNMRDPVMYRILHQSHHRTGDKWCIYPMYDFAHGYEDAIEGVTHSICTLEFE
                     DHRPLYDWFIENVSVPHRPHQYEFARLALTYTVMSKRKLLELVNKGYVSGWDDPRMPT
                     ISGVRRRGYTPASIRTFCKKIGVSKANSLVDVELLHHCIRDELNQTARRVMAVLKPVR
                     LVIENYPEGQFEEIEALDNPEASIATFRKLPFGRELFIEMDDFMEDPPKKFFRLSPGA
                     EVRLKHAYLVRCNGFDKDSDGNVTEIRCTYDPDSRGGEAPDGRRVKGTLHWVSAPHAV
                     DAEVRLYDHLFTLRDMDSMEEGRDYKDYLNPDSLQRLTGCKVEPSLAEAGPMERFQFM
                     RNGYFCTDPEHTRKHPVFNRTVSLKDSWAREKDRK"
     gene            1517..2932
                     /gene="tilS"
                     /locus_tag="P5541_00595"
     CDS             1517..2932
                     /gene="tilS"
                     /locus_tag="P5541_00595"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRV70503.1"
                     /translation="MKGKEQYSEPTGSGSGSVLKRFRFAGKNQEWWDSGKTVLAAVSG
                     GSDSMAMLWLLRYYWKGRVLVAHLEHGFRQETALRDACFVENICRDWGIDCRVEHRNV
                     PSLKKPGETLEEAGRRERYDFLLRVASEAGAEFVATGHTADDSTETVFFNLLRGTGIR
                     GLRGIPEVRGNIVRPVIHCFREELQSFLSERSVQWVADESNDDVSYFRNRIRHVIFPF
                     IHKEGNPRFSEHLLALSEEVSEAEKVRDKRSVSLAAWARRPFPLAIRVWDRKTLLRMC
                     KGDLQSLFAFEGRSLALSPLSRVKTARLLELVQGSENPWRFQWERDLEICGSGKIVAL
                     VKRALAEGASPPEKIFGFPEVAGSIRWGPWKLEWFPVSGEATPSDGDFSCIMPVSKGS
                     LLKVRSMKCLDKTCNRKARVPWWCTGTWPVVQSGGYLWSPIEGFIGKPEVTSAALLFR
                     FRVCETRDLRKGDHGHDLCAG"
     gene            2913..3446
                     /gene="hpt"
                     /locus_tag="P5541_00600"
     CDS             2913..3446
                     /gene="hpt"
                     /locus_tag="P5541_00600"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRV70504.1"
                     /translation="MTYVLGDVLLGRKDIDQKVREMALDICTAYGEKDLIVVGVLKGA
                     VVFLSDLIRCFPVSMATDIDFLAVSSYGDSTESSGVVRITKDLDINITGKNVLVVEDI
                     VDTGLTLSYLMNILREREPEMLDVCALLDKPSRRKTDIEITFKGFEIPDEFVVGYGLD
                     YSGRWRNLPEIYTLNEE"
     gene            3497..5380
                     /gene="ftsH"
                     /locus_tag="P5541_00605"
     CDS             3497..5380
                     /gene="ftsH"
                     /locus_tag="P5541_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRV70505.1"
                     /translation="MGKMTKNLGLYLVMIVLVVSLVNMFLSPAQGPEEFESISYSAFL
                     NYFREGKLEQVSIEGDTIKGTREGGVKFSTVAVGIGDLAPQLAEKGVQVTVLPPARSP
                     WWVTMMSSLFPTLLLIGAWIFILYHMQGGSNKVMNFAKNKAKLFLDNRPKVTFEDVAG
                     CDESKEELKEVVDYLRDPGRFSRLGAKIPKGVLLLGPPGAGKTLLARACSGEADVPFF
                     SISGSDFVEMFVGVGAARVRDMFEQARKNQPCIVFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFDVSSGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDKNGRAEI
                     LKVHLKEMKVEENVDLDVLAKRTPGFVGADIANLVNEAALLAARLDKKSIGMSEFEEA
                     IDRVLAGPERRTRLISEREKEIIAFHEVGHALVAKKVPGCDPVHKISIIPRGHKALGY
                     TLQLPEEDRFLMSKVELTNRICVLLGGRVAEQIHFDDITTGAQNDLERATQIARQMIT
                     EFGMSESLGPVTLGRKQHEVFLGRDIVEERNYSDEIAYSIDKEIRKIVDDCYERVRNI
                     LTDNHDAHVRIARTLLAEEVIEGDRLDSLLEEGIPVVPEDSEGETEVAVGSSAEQDGS
                     EPV"
     gene            5494..7533
                     /gene="uvrB"
                     /locus_tag="P5541_00610"
     CDS             5494..7533
                     /gene="uvrB"
                     /locus_tag="P5541_00610"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRV70506.1"
                     /translation="MEQDRHPFNLYSEWPPAGDQPEAIEKLVNGFNRGDRYQCLLGVT
                     GSGKTFAMANVIERLQRPALVLAHNKTLAAQLYSEFREFFPENAVSYFVSYYDYYQPE
                     AYVPVSDVYIEKDASINERIEKLRLATTKSLLERRDVIVVASVSCIYGLGRRKAYEDA
                     ILRFSVGERWERRDLMMKLVANHYERNDLVLEPGNFRVRGGILEVFPAYSDQAVRIIL
                     EEDLIERIEEIDPLSGKRILSKPNAAIFPAQHYITSPEAIAEALPEIQRELEAQVALF
                     RSQGKLIEAQRLEMRTRYDMEMLSQVGYCSGIENYSRILDGRNPGDTPGTLMDFFPED
                     FLFFVDESHMTIPQVQGMFNGDRARKETLVEHGFRLPSCLDNRPLKWDEFREFLCQAC
                     FVSATPGDWEMDVSTNVAELIARPTGVVDPEVEVVPATGQVDDLTERLRSVVSRGERA
                     LVTTLTKRSSEDLAEYLSEIGFGVKYIHSELDAFERTELIRDLRRGDISVLVGVNLLR
                     EGIDLPEVSFVGIIDADREGFLRSRRSLIQIIGRAARNTSGTVVLYADQMTGSIEAAM
                     DETNRRRRKQLAHNLKHGITPVSIKKKIMALLPEELLEDLGSDLVTKPGGEGFTRQEL
                     EEVMWKAVERLDFEKAAAIRDILHGEKGGEVSRAAMDRSKGRKTAQSQKHRRRHS"
     gene            7463..10303
                     /gene="uvrA"
                     /locus_tag="P5541_00615"
     CDS             7463..10303
                     /gene="uvrA"
                     /locus_tag="P5541_00615"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRV70507.1"
                     /translation="MPQWIEVRGARQHNLKNIDVDIPKNRFVVITGPSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGVQDKPDVDDISGLSPAISIEQKGVSHNPRSTVGTV
                     TEIYDYLRLLFGRAGIPVCPLCGKPVEKHSVDEILDILFQKNESKKVDILSPVVRGKK
                     GEFRNLFLQYQKEGYLRARVDGTVLWLEEEIPLDKNRKHDIEILVDRLTVMPEKRERI
                     AEAVENALSLSKGYVLLAFEGEETIISEKFACPGCDITLPEIEPRLFSFNSPFGACPD
                     CNGLGSHQYFSEELAVEPSVSPFEGALLPWKNKHYMLRKLKSLALHTGWKMDSPFRDL
                     EPEVREAILYGYSVKIPLTFREGRKEMSYMGRYEGLIPWLETRWKDTESESVRNEIAS
                     FRAEDVCQSCGGRRLRPEALSVKVDGLTIGEVVDMPVSELLERLMGARVHEFMIEPAK
                     RIIEEISKRLSFMVDVGVGYLTLSRRADTLSSGESQRIRLATQIGSKLSGVLYVLDEP
                     TIGLHPRDTTRLLGTLEAIRDLDNTVLVVEHDRDTMTRADYIIELGPAAGEFGGEVTA
                     RGKPKEIARTSSLSGPFLTGRESGLSIRKKHLKAPEGFINIRGAGENNLKNIDVKFPL
                     GRLVCISGVSGSGKSTLMNEVLYKGLRRKLDRNFRERAGKHTDIEGWEALKNVIMIDQ
                     APIGRTPRSNPATYTQLFTPIRELFASLPESRIRGYGPGRFSFNVKGGRCEACRGDGE
                     VRISMLFMPDVYVKCDVCNGKRYNRETLDVRYKGKNISEVLDMPVEEAGVFFGDIPRI
                     ARRLAVLKEAGLGYMRLGQPATTLSGGEAQRVKLAEELGKKFKGHTIYLLDEPTTGLH
                     YTDVRKLIVLLQKIVDHGNTVVLIEHNLDVLASADHLVDLGPEGGGGGGKVMDTGTPA
                     EISKKARGHTGRFLAEYLDQLRGREDNDRAAG"
     gene            10284..11069
                     /gene="rlmB"
                     /locus_tag="P5541_00620"
     CDS             10284..11069
                     /gene="rlmB"
                     /locus_tag="P5541_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRV70508.1"
                     /translation="MTERQDEKNTMDNDICFGRNSVRSAVASTPDLCSRVMISESLSG
                     KEKQEITSLCRDSGVAWQMVKPALLDNLVPAGRHQGFAVRLSAVPLLEVEDLESRKKL
                     FPGPDLLLLLDHVQDPQNLGAVARSAEVFGAGAIILPRRRSAMPTSTVMRTSAGAIAR
                     VPVAGVVNVSRTLETLKKWNYWIVGLHHESRQVLGSIPLPERTVLVVGGEDSGISRLA
                     ARSCDHLLRIPMVGSTGSLNAATAAAIGMYEWRRAVDKGVVHE"
     gene            11090..11164
                     /locus_tag="P5541_00625"
     tRNA            11090..11164
                     /locus_tag="P5541_00625"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11122..11124,aa:Val,seq:gac)
     gene            11260..11625
                     /gene="acpS"
                     /locus_tag="P5541_00630"
     CDS             11260..11625
                     /gene="acpS"
                     /locus_tag="P5541_00630"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRV70509.1"
                     /translation="MIRGIGIDMCSIPRLARALGNPRFLDRVFTVEERDYVARSGGNP
                     RHYASAFAAKEAFAKAGGWGIAVVGLRNVWLSRDKDGPSLMLAPHAMSLVESIKAATA
                     HVSVTHEGDFAVAVVILEG"
     gene            11622..13181
                     /locus_tag="P5541_00635"
     CDS             11622..13181
                     /locus_tag="P5541_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRV70510.1"
                     /translation="MTIIRYLFDPVDIREADRRAMSEIGVPGTILMENAGRGVADVMT
                     ARFPTARKFLFVCGPGNNGGDGLTAARHILVRGKEAVVILACAEGKLSVESLRNLDIL
                     KNIGCPVRYSSFLSTEEITASALENDIVVEALLGSGSSGAPRGEILRVIKAIPGSATV
                     VAVDVPAGVDPSDGRVEGFALRADLTVTMLAEKTGLEIMPGREHCGDIEIVDIGIPPA
                     SVLPEKSGVSIFLPSDAGRLLPEWSGTTHKGSRGFVLVVGGSSRYWGAPVLSALGALK
                     AGAGCVVVAAPEESAAFAGGYPEMIFTETICDGGFLSPATWDLVIEQWGGRIDSIVAG
                     PGMDRGKPAEELFTRIWKEWEGSLCVDGDALHFLGKGQGPFPLRGNTVITPHEGEAAS
                     LLSRERPSVSASRLEAVRTLFKNFGTCVLKGASSLVCGDSGVRVIPYNLPGLSVPGSG
                     DVLSGVAGAMLGMGIAAEDAAASSACLHAIAGSFLQEERGFNGILAKEIAMALPMAIK
                     FARSGAGLNSH"
     gene            13184..13693
                     /gene="tsaE"
                     /locus_tag="P5541_00640"
     CDS             13184..13693
                     /gene="tsaE"
                     /locus_tag="P5541_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRV70511.1"
                     /translation="MKGQIIVTGSAEETRMVGEQMSGVVVPGEYVLLYGPLGAGKTEF
                     VRGFAKGCGWEEVRSPSFTIVNEYYSEPPIIHVDFYRVDRASSEEFALEEYAAEGCVV
                     IVEWADLWESPDDLPAWEVRFSPCEEEQPGQDDRKRYIEIFRRGKELPSILQSRFQVS
                     DRRGKRMEK"
     gene            13690..14361
                     /gene="tsaB"
                     /locus_tag="P5541_00645"
     CDS             13690..14361
                     /gene="tsaB"
                     /locus_tag="P5541_00645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRV70512.1"
                     /translation="MIVLGIDCSTKGTNVGVARNGTIISEYNVSIGRRQSADLPWLVE
                     KALSDAGIDLRELDLIASSSGPGYFTGIRVGISYAAALAEGLEKKIVLPDSLHCLAAP
                     FLDGNRRVMSVIRSRKGFFYYSVLEGQYSERRLITPPSLESTAELMNICGIFNPSVLV
                     LDGDPVFREEMKIPDSVYAEWVSVRGGWVALIGESLSKNAIPPSEARGYYIREPDIGQ
                     RKFHE"
     gene            14523..14843
                     /locus_tag="P5541_00650"
     CDS             14523..14843
                     /locus_tag="P5541_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV70513.1"
                     /translation="MDLIEKHPILDFNHGEKVKFTFDGAEMEGFEGDPIAAALHANGV
                     RVYRETPIKKQTRGFFCAIGKCSSCFMVVDGVPNVRTCVTPLKSGMRVETQRGKGVIA
                     IDAE"
     gene            14861..15973
                     /locus_tag="P5541_00655"
     CDS             14861..15973
                     /locus_tag="P5541_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV70514.1"
                     /translation="MRQTEVLVIGGGPAGLSAALEAASLGCEVTLVDSDLALGGQLIK
                     QTHKFFGSKEERAGTRGFVISRMLLEELDTFKEKVKVFSNCTATGFYKEDGVVSCVVG
                     EEEYFRIKPKKIIMATGAQERLLPFPLNHLPGVYGAGAVQTLMNVYGVVPGKKVIMVG
                     AGNIGLIVSYQLMQAGVEIAGIVEAMDKVGGYWVHAAKVRRLGVPIYLRHTIREALGD
                     QVVSGAIIQEIDEKFQPTGKEIKVDADVICMAVGLTPTCELLWQAGCEMAFVPQLCGH
                     VAQRDLNMRTSHPDIWVAGDAAGIEEASAAMVEGRMAGVGVAKSLGHGDARDLSKRME
                     EYRRRIGDLRGGEVGQKICQGLQCCTVDEWGVCSID"
     gene            15966..16475
                     /locus_tag="P5541_00660"
     CDS             15966..16475
                     /locus_tag="P5541_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRV70515.1"
                     /translation="MTDKVKLFNGGVLTEPRKGAIEPPKELWEGKKNGLVIIECPQRI
                     PCNPCHTSCPTGAVKPFKDINDQPEIDYDKCTGCARCVAVCPGLACIVVDLTWGGSDV
                     ALMKLPYEMLPLPEEGQEVECLNRVGEEVAKGKVVKVTEPMNDRTKVVHVEIPGSLVM
                     EIRTIRVVK"
     gene            16490..16762
                     /locus_tag="P5541_00665"
     CDS             16490..16762
                     /locus_tag="P5541_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV70516.1"
                     /translation="MTNDIVICRCEEVTLGEIREWIAKGYETFDELKRVLRVGMGPCQ
                     GRGCREMILREIAQKTGKTVADIDPGTFRQPSKPVKLAVLAEGGDK"
     gene            16762..17913
                     /locus_tag="P5541_00670"
     CDS             16762..17913
                     /locus_tag="P5541_00670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV70517.1"
                     /translation="MTWKRTADVVIVGGGVQGCSIAYNLAKLGVRNIVVLEKGTICSG
                     STGRCGAGIRAQWGTEMNCRFGLASVEKFEQLHEELGMDCGLNQSGYLMVAYKESEFE
                     QLQKSMKLQNSLGINTRVVSYDEAREICPGLAAEDAVGFTFHNRDGHADPFLTTFAFM
                     EAAKRLGVVFHRFTEVTDIRTVAGKVAGVSTTAGDIDAPVVVNAAGGYSQFIATMAGV
                     EIPNFSERHEIIITEPVEAGVCPPMLMSFSGNYYIQQRPHGSIIGGCSPEGHPQDYEL
                     GASWDFIEMMSRTFVKLLPRTKGVRVVRQWSGLYNMTPDKQPILCEADNVKGFYLSVG
                     YSGHGFMFAPISGELLAQKITGKTPWISIDMLHYDRFEKGELLVEPAVV"
     gene            18148..18363
                     /locus_tag="P5541_00675"
     CDS             18148..18363
                     /locus_tag="P5541_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV70518.1"
                     /translation="MPKRFNVEVNSAWCKGCGLCMAVCPKKVFDFDGNLKSTPSRVQD
                     CIGCKQCENICPDLAITVEGSGEDAQK"
     gene            18350..19486
                     /locus_tag="P5541_00680"
     CDS             18350..19486
                     /locus_tag="P5541_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRV70519.1"
                     /translation="MPRNEFWQGNVSLAQGALAAGCRFFGGYPITPSSEIAETMAEEL
                     PALNGRFIQMEDEIAGIAATIGGSVAGYKAMTATSGPGFSLKQENLGLAYMAEIPIVV
                     VDVMRGGPSTGLPTSIAQQDVMQARWGSHGDHATIAYAPSSVQECYEIAIKAFNMAER
                     FRQPVLIMSDEVLGHMREKVVVPEEGSFPLVNRKRPSCDPENFIPYSPDPEDNLPPMA
                     SFGDGYRWHITGLTHNEWGFPTNCASEIAKKSARLMDKVSAYRDDIVEYETESVDDAE
                     IIVLAYGSVARTALSAVRQARKNGMKVGYFRPITLWPFPDREIEALAGKCASILVPEL
                     NFGQMILEVERAVHGKADVVQMSLVNGELFQPSEILEKIEEVAR"
     gene            19486..20307
                     /locus_tag="P5541_00685"
     CDS             19486..20307
                     /locus_tag="P5541_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV70520.1"
                     /translation="MPRPEVFDWLRKKFFPHMWCPGCGHGIIMHAILRALIDSGKKRE
                     ETVIASGIGCSSRMPGYLDCCTMHTTHGRSLAFATGIKLANPELTIIDVMGDGDCTAI
                     GGNHFIHACRRNIGITAVVMNNNIYGMTGGQASPTTPLGAKATTAPYGAIDPPFDVCK
                     LAQGAGATYVARATIAQPLLCEQYLTHGIANKGFSVIEIISSCHTQFGRKNNRRTPVD
                     NLNYLKNASVPLARSETMTPEELEGKIVTGEFVKRESPEYTDLYLDLISRVRGGK"
     gene            20307..20861
                     /locus_tag="P5541_00690"
     CDS             20307..20861
                     /locus_tag="P5541_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV70521.1"
                     /translation="MSDRYEIRFAGSGGQGVILAAVVVGEAAAIFEGLNAVQSQAYGP
                     EARGGKSKSEVVISRDGIDYPKATAPNLQIILNQTSCDEFVADTAKGGVVIADDTYVN
                     SVPNSDAEVYMLPIVKTAREKIGRELVTNMVALGVTARVLEAEGMMKPESLKKAILQR
                     VPKGTEEMNERAFNEGYELMKAKK"
     gene            20968..22389
                     /gene="rlmD"
                     /locus_tag="P5541_00695"
     CDS             20968..22389
                     /gene="rlmD"
                     /locus_tag="P5541_00695"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRV70522.1"
                     /translation="MKDSEGKIISANVETVNSRGDGVVRSGDDRFVFFVPGALPGERI
                     TGKVVIVKKSYGVLDLLEVKEPSPERIEPRCPWYGRCGGCALQHASYGFQLKMKDTVV
                     MDAFRKVASMVLPGFEGCVPSPLQWGYRNKASFPVQMSKGVARAGFYSQKSHDLVPVD
                     VCPVLEERLESWIGPIGSALKRSGIQAYDESEHNGLLRHIVARCAGRTSSSMVVPVVC
                     LAPGKDIPAELKNLADMFMEEFSGVGGVNINFNPLRGNTIHGRYSRLVAGDGSITDNI
                     CGFSVKYGPTSFFQINSLQAEFLFKAAVEELDLLGCERVLELYSGTGALTLNLARGRK
                     KVHAVEEWPESAGYLRENALINGVENIHVLEMSSERGVQFLGRAKFDVVVVDPPRKGC
                     SEHVLKGISAIDPEYVIYISCNPSTLARDCAFLMEKGYRLRNIRTFDMFPQTFHVETI
                     ATMVKERRKKRKPGPVAGKVRES"
     gene            22630..22704
                     /locus_tag="P5541_00700"
     tRNA            22630..22704
                     /locus_tag="P5541_00700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22662..22664,aa:Gly,seq:ccc)
     gene            22915..23247
                     /locus_tag="P5541_00705"
     CDS             22915..23247
                     /locus_tag="P5541_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HRV70523.1"
                     /translation="MAKNIADEITEAEAKGKQMVQDARTEGARIFAQAKSESEDMVKA
                     AKQNFHRSFREAVVKLEAEAEVEAEKIVEKGRAEAEKYARAHESNIDDAAAWVAEEVI
                     GRYGSNKV"
     gene            23228..25255
                     /locus_tag="P5541_00710"
     CDS             23228..25255
                     /locus_tag="P5541_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HRV70524.1"
                     /translation="MAVTKFKKIRILSHHEALDHVIAEIQKIGCCEIIDRDDGGNETG
                     SVVSTSDRSRDRGSLDLDSSLSDVRFILRFLEPLYKEEGGKLERILGPKPSVSFSDLS
                     LLKKSTDISMINSEARAIERQLTETRSELAQIANQSLLLDSIKGFTLPLWMISRGTGK
                     VMGVMGTIASSQASALESDFSNASQGEGHIFIAPVLEKQPETNAVLVFSRSRESAVME
                     LAVKNGFSRIECPQGLEMTPGEELERLEGLAAELKTRLEEAEKKAYSLAQSWVPIVRK
                     LNDFLNVSIARRDAAGSGESTTMVSILRFWVPADRTGDLEKALAPYSGLIDVLTEDPA
                     PEDHPPILLDNPLWARPFGPLTKLYGMPSYKGIDPTVLMAPFFFLFFGMCLGDGGYGL
                     IMGAFFLFALVRYKLSGDTKNFFVLLLLGSVSTIMVGAVTGSWLGDMVDAFGIFSFIR
                     PLKESVVLLRPMSDPITFLGISLALGVVQILFGILIAMWDALRKGDIIAALGDQGGWF
                     MLIVGILVWGAGSMGMGGEGLILTGKGLAALGALTLVATQGREKPGVIRKAVFGVLSL
                     YNVTSYLGDVLSYSRLLALGLATSAIAMIVNTLTNLVSGIPYVGWVLAALIFFGGHLF
                     SVVVNVLGAFIHSLRLQYVEFFSKFYSGGGRIFSPLKFETRYTNITDGTIE"
     gene            25324..25800
                     /locus_tag="P5541_00715"
     CDS             25324..25800
                     /locus_tag="P5541_00715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRV70525.1"
                     /translation="MDQLGIMLSVLGAALAAGFAGSGSAIGVGIAGEAGAGIMTEDPG
                     KFGLVLLLQALPGTQGIYGLLTAFFAILKVGLLGGSAVPVNVWQGLGVLFACLPIAIA
                     GYFSGIAQGKTSAACIQMIAKRPEETGKAVILPAMVETYAVLALLMSIILLNSIQF"
     gene            25828..26409
                     /locus_tag="P5541_00720"
     CDS             25828..26409
                     /locus_tag="P5541_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRV70526.1"
                     /translation="MSLEDIKARIEADAKAEAEGITGKFRDQADEVMKVAKAEVDKLE
                     KATQSKIKTEETEILRRRKIVAELDVRKIQLGTKRDLIDKAFQKALSILSKMPGEKYV
                     PFMTGLLDRSVSSGDETVYVGKEEKILDEKWISDFNSRKKTKLTLSSEWVPASGGFVA
                     RRGDIYVNCTWDVLLRRIREDLETEVVNRLFPN"
     gene            26425..27435
                     /locus_tag="P5541_00725"
     CDS             26425..27435
                     /locus_tag="P5541_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRV70527.1"
                     /translation="MTRSIDYGYSVSRLRAMENRLLDPGSFQRLLDSEDLSSAMRVLS
                     ETVYGKWMLEQQREEQFDQAIESELQYAYSETEKFVPDPGLYQLCRLQYDIHNIKVVL
                     KGIFVHKQGGKRRMDLLTGLGNINVDDLVVALESEDYRLMPFGFHRTVPECVMLWEQT
                     HDILQVETVLDRKLFTLQSDLASSMPFEGARLFVKHRIDSENIRNLARLKRMGYEAAQ
                     ASPFFHPGGFVSAEKIVSMIGEPLEGWERILSFADVSRALSGAQEHSGIDSLLVDLEK
                     SIDDFLSSVLEQFKYSPFAPENVLNFLWKKEIEAKNVRILLVGIGNGAERSVLRRLLR
                     HV"
     gene            27428..27757
                     /locus_tag="P5541_00730"
     CDS             27428..27757
                     /locus_tag="P5541_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRV70528.1"
                     /translation="MSSEVKKLKMAAVGDFESVLPFQAVGVTPFIPEGGLTELLMRLS
                     REKYAVVFLVDDLYAQNRESIEEINETQKISIIPVPGIKGTTGIGIKTIRDSVERAVG
                     MDIFAVK"
     gene            27803..29590
                     /locus_tag="P5541_00735"
     CDS             27803..29590
                     /locus_tag="P5541_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRV70529.1"
                     /translation="MKDKIVGSIAKISGPLVVADGMSGSGMYDVVRVGDLGLVGEIIE
                     LKGSTASIQVYEETSGLKPGEPVESTGAPLSVELGPGLIEQFYDGVQRPLALIEKAAE
                     SPYISRGINVSAIDRSRKWEFIPSVEPGTELCEGDFIGYVQETVLVKHMITVPHGVKG
                     KVKKIESGSFTVEEVIAVIDSGDKDVDITMLKKWPVRTPRPVSRRLAPNIPLTTGQRV
                     VDTFFPLSRGGAACCPGPFGSGKTVIQHQLAKWAEAEIVVYVGCGERGNEMTDVLLEF
                     PELEDPRSGHPLMKRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYSVALMADST
                     SRWAEALREISGRLEEMPGEEGYPAYLGTRLASFYERAGRAICLGSDSREGSITAIGA
                     VSPPGGDLSEPVSQNTLRVTKVFWGLDANLAYQRHFPAINWLLSYSLYSEKLGEYWDN
                     KYDGEWSGFRTEAMTLLEEEDRLREIVRLVGIDALSRQERMILETAKSLREDFLHQNA
                     FHEIDTFASMDKQYRMLRNIIGFHHLCMDAIEHGSTIQDLFSLPVREGIAKMRYVDEK
                     ELDKIDELLPEMKKQVEELSPEGGMGDVA"
     gene            29580..30980
                     /locus_tag="P5541_00740"
     CDS             29580..30980
                     /locus_tag="P5541_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRV70530.1"
                     /translation="MLPKEYRTISNLSGPLLVVENTENVRYDELVEIELVNGETRRGR
                     VLEITRDHALVQVFEGTSGIDLDSTKVRFMGKVLTLPVSKDILGRIFNGRGEPIDDGA
                     PVIPEKSLDVNGYPMNPYSRDYPSEFIQTGVSTIDGMNPMVRGQKLPIFSGSGMPHNR
                     IAAQVARQATVLGGHEDFAVVFAAMGITFEEASFFMEDFRKTGAIERTVMFVNLADDP
                     AIERITTPRLALTCAEYLAFERDYQVLVILTDLTNYCEALREISSARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKKGSITQFPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIYPPVDVMPSLSRLKDKGIGEGKTREDHADLMNQLFAAYARGKEAKELATILGE
                     GALSEEDKAFALFSDRFEDSYVRQGEYENRTVQETLELGWELLTLIPVRELKRVRDAW
                     VEKYLKPRLKESGEQR"
     gene            31007..31633
                     /locus_tag="P5541_00745"
     CDS             31007..31633
                     /locus_tag="P5541_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRV70531.1"
                     /translation="MAKKLSVNPNRMELSRLKIRQKVAKRGHKLLKDKQDALIKAFLE
                     KAKNLKELREKVEKELEECYRSFLLARAQTFPAMLEQALMITGSGLSISMSNKNIMSV
                     IVPVFEVQQGEATLNYGLGTTTGNLDVALEKFSRLLPEMVSMAAEEKALRLMAVEIER
                     TRRRVNALEHVLIPSFQETIRYITMKLDEQERANLSRLMRIKEIVRSH"
     gene            31725..32145
                     /gene="rnpB"
                     /locus_tag="P5541_00750"
     ncRNA           31725..32145
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5541_00750"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            32341..32766
                     /gene="mraZ"
                     /locus_tag="P5541_00755"
     CDS             32341..32766
                     /gene="mraZ"
                     /locus_tag="P5541_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRV70532.1"
                     /translation="MLVGTYEHRFDPKGRIVLPSKFRDELGENPVATVGIDQCVSIYP
                     ADKWQALLERFQKMSFSKGRTRDFLRVLLATAHEIQFDASGRVLVPVNLRQHARVEQD
                     ATVIGVGDHIEIWDSGSWNIYLEGVKKELPDIAEEVEGL"
     gene            32763..33689
                     /gene="rsmH"
                     /locus_tag="P5541_00760"
     CDS             32763..33689
                     /gene="rsmH"
                     /locus_tag="P5541_00760"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRV70533.1"
                     /translation="MSGHIPVMLPEVCKVLGSLEKIGFVLDATLGFGGYSEAILFKWP
                     NVAVLGVDQDPQAIESSKRRLAPFGERFRAVHGNFRDIGEILERSGMGVPDAILFDLG
                     VSSVQVDTPERGFSFQEAGPLDMRMDTADERMGMAADIVNNYGMKDLIRIFRDYGEER
                     FAAQIARGIIKYREKEGPVNNTDTLVGIIRNTLPAPVQRKMGRNPARKIFQALRIAVN
                     DELKALEEALEQCGRFSKDRVTLVVLSYHSLEDRIVKRKFLEWKGLSLGVLPFRKPLV
                     PGEEELEENRRSRSAKMRVFHFGLKDPGGKKR"
     gene            33686..34111
                     /locus_tag="P5541_00765"
     CDS             33686..34111
                     /locus_tag="P5541_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70534.1"
                     /translation="MNGQNPVLEYSGNGRIRGRGSICIMICVVTVALLMGLVGLRLYC
                     LNLEYRLAGIQNQIVAREEEQSRLEKDLASLLNPTRVYSYARSELGMRASANIGLVRI
                     AGTESRPSGPVSPGPETGKFRSALVRLVDAITGKASARQ"
     gene            34147..35793
                     /locus_tag="P5541_00770"
     CDS             34147..35793
                     /locus_tag="P5541_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRV70535.1"
                     /translation="MISLRKSPWFFMFLCVLAILFRLYFLQMEPDPRVRSQASSQYWA
                     RLKVSAQRGYIRDSNGISLALSTPAFSFFIDPTIWDPTNAPLLKDHISGTKLEKISGR
                     MEGRYYQVARKVTPDDAEKMIRMKLPGLFWVKETRRVYPQGRTLSHVLGYCDIDDEGL
                     AGLELFWNHALYAPPESRIIAKDAKGNFVDVARPGKPVAPEGQGSVGLTIDSRLQHIL
                     ERRLREGVSKFKAEWAAAVLLEPDTGRVLAMSSLPDFDPNDRSTFSNGESLRNNVISR
                     VFEPGSTFKPVILGNVIDNGQVSKNTSVTCTGKIKVADVVIHDIKAHGKIDMTRVIAD
                     SCNVGMVMSVEKSEPLQTWSFLRRCGFGSISGVEIPGEESGLMTPPSQWRGSIPATIS
                     IGHGIGVTPLQLAVAISAIANGGRIMKPFMVSEVLSREGNVLYAGKPTVMAEVFSSDT
                     AKWLRSAMRKAVENGTGKNADLPGAHIAGKTGTAQVPGSGGYIKGSYTPSFAGFWPSE
                     DPGFLLLVVIGNPGSGDFLGGKVAAPVFRNIVQDIEQISR"
     gene            35824..37359
                     /locus_tag="P5541_00775"
     CDS             35824..37359
                     /locus_tag="P5541_00775"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRV70536.1"
                     /translation="MNLRELTDMLASKGALRQVVQPLVSVTEEIGDPGDKNISEITHD
                     SRNVIPGALFACIRGGKQDGHLFANEARKRGASALLCENDTGEEIVHLIVDDARRRVG
                     EVASAVYGNPSSSMKMVAVTGTNGKSTTSFMLRSIMGASGEKAGIVGTIVYDDGSGIE
                     MEANRTTPEATDIQRLLLNMSQNGCNSCVMEASSHGLSQYRLSGCLFDGAVFTNLTPE
                     HLDFHGTMENYFASKVRLFQDHMKGSGWKVSVNNDDPYGRVIREMFKGNVLSFGLEKE
                     FSPDYLGEIEGMDLEKIEMTVYGPFNGSPIRLKLPVTGSFNARNALGAASLSFAMGYS
                     AENIIKGLENLPQVPGRLQRVLLPGGVTALIDYAHTPDALFNVLSAVREVCNGEIWSV
                     FGLGGDRFHENRPVMGDIASRIADHLVITMDNPRSEDPASIADQIVAGIPSKDDKKRG
                     DRVIILDRKEAVEYALDRAATGDAVLISGKGPEKNIVFSDRVIPYSDLEAVEEWLKRR
                     GSK"
     gene            37356..38771
                     /gene="murF"
                     /locus_tag="P5541_00780"
     CDS             37356..38771
                     /gene="murF"
                     /locus_tag="P5541_00780"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRV70537.1"
                     /translation="MNRFYQCDEWTLSKAAKLTGTRWSGEDVHLCRPFRVDTREIQAG
                     DVFIALPGENSDGHFFLDDAIEMGAGALVINPALTGPVMDRILDEKIPVLFADNTGRA
                     LKELARKRLDILKPECVMAITGTVGKTTTREMVRAVTESRGDVHCARRSFNTWIGCSL
                     TVLDAPPGTKVIILEMGTNHPGEISEMATLFRPDYGIITEIGEGHLEGLGDIEGVLSA
                     KMELAEKGELKVLSYNIDNNSLSRSVGSLPAKIKRISVGRSDSHYRIISSTFGLVKEK
                     PLLSVEVMTPKGLRKVSAELFGDHNALSMAFAMAAGDYLGIGPEIQVRALEQFNPMPG
                     RGVVRLLPGGGIGIDDTYNANPLSMTQALRTISSMTPSPGSKRMAVLGGMGELGDISG
                     MLHQNIASLFEGLDEVYLYGETWKTSMKGRFPYNTRHFNDIEEIATVLQERVFPGDVI
                     LFKGSRSFKVERVIEILEGTR"
     gene            38768..39739
                     /gene="mraY"
                     /locus_tag="P5541_00785"
     CDS             38768..39739
                     /gene="mraY"
                     /locus_tag="P5541_00785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRV70538.1"
                     /translation="MMGSVLFFAGVLVLGVILQEFWVKFLQKRNISQTMKEYGPQNHI
                     LSKKGTPTMGGVIFLFLGVVISFVFCLAGFWNTRLAMEIMFFPIAGGLVGLGDDLLKY
                     YRVSSEGLKSLQKLVLQFIVAVLWAVFFLSWRDLSLFPGYEIGGITAFLLIVFFLTGG
                     LNAVNITDGLDGLASGACAISFAAFILLAGAGNPAFAGALSGLAISTGFLFHNSFPAR
                     VFMGDCGSHFLGGLLVSVCMAGNFTLLLLPLGFIMGIEVITVIIQIVAIKGWGRKVFL
                     MSPVHHHFELAGWSEKRVVWTFWGAHIGGLLIMSVPVMIIADRFGGA"
     gene            39741..41147
                     /gene="murD"
                     /locus_tag="P5541_00790"
     CDS             39741..41147
                     /gene="murD"
                     /locus_tag="P5541_00790"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRV70539.1"
                     /translation="MKGSFETSVKARKKDFSVTVVGAGVSGKSLAILARNLGYRVFVT
                     EARDIDEETVGSFRNYGIFYEEGEHSPKMLECDLVVLSSGVSPKSEPVVMAKSRGIQI
                     MGEADFVSPYLKGKIIGITGSNGKTTTTMLTGHLLEKAGFRTGVAGNVGSPLAEHAGI
                     DYDVVVAELSSFQLYWTSDLRTDVSVVTNVDPDHIDWHGSLEEYHRCKRKILAMLRND
                     GKVICRGKDLEALYPEGFGKEGLYPLFWEEDGSLYDRGHILMLSEEAELASGEGRSVL
                     FRYKDLPLLGRHNHENAAMAVTAFSMFANDLSTVSASLENFKAPPHRCELVAVIKGVS
                     YIDDSKGTNVAATVTALKSLKGRKTVILGGKGKGEDYAPLASAVASEAAFAILMGDEK
                     DNIRAALDEVGYKFYQEAEDMNDAVEKASLSTREEGGMVLLSPACTSWDRYPNYKERG
                     MDFRRNVAKLVERNIDAG"
     gene            41137..42255
                     /locus_tag="P5541_00795"
     CDS             41137..42255
                     /locus_tag="P5541_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRV70540.1"
                     /translation="MQAKVDQIPARHRPLWGLAIQGTWVIPLVLSCIGILMISSVTAQ
                     LTVTQGRTPFYYGLRQIKWLGAGLVMMIACTVPTTEFWKKWSGVIWLGSLFLMVFTLI
                     PGIGREGGGSNRWVRFGPFSIQASEVMLLTFALHLTKKLREAGLDRKKGMARTIVLFV
                     LSSWPFAFQPDLGGALIMFVLSMGIYVQVFGWFLPIVAGISAMIGLFVPFVFLSRYRF
                     ERLMAFHDPWKDPLGSGFQTLQGFVAFANGGGFGVGIGHGLQKLQYLPAAHTDFILAV
                     IGEELGLAGTFTLVILFGVFFLIQFLSYLTLDDDFDATLVWAMTLAIFIPFMVNAGGV
                     LRILPLTGVPLPFVSYGGSSLVLGWCKVGILLRLLRNV"
     gene            42256..43332
                     /locus_tag="P5541_00800"
     CDS             42256..43332
                     /locus_tag="P5541_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRV70541.1"
                     /translation="MKVLLVAGGTGGHIWPAIAFALWLNEKNSGNDTRFACGSRSLER
                     EIFAHEGIEPLFLPIEGSPLWGDLRTRFSRWRGMWKSFWISAGLLREWEPDVCILFGG
                     YVSFPVIIASLVTGTPFVVHEQNARAGRVTRTASLAGSPVFSGWKECLPLGEGKFVRS
                     GIPVRSMTRMAPEIAWRKLGHYEEVPPSPRVLVLGGSLGSDPVSQIFAEASTMEPFSG
                     WSIIQVSAVKEPVKRGVNHWILPREWDVGLLYSLADVVVSRAGASTLAEIVEFGLPSV
                     IIPWRGAADDHQFENARLFKEQGHGSIWEINNGLLPNLGFMIDYELKRARPGQETCPE
                     SGNFESCEILWRYVQQYAEGRGRN"
     gene            43329..44774
                     /gene="murC"
                     /locus_tag="P5541_00805"
     CDS             43329..44774
                     /gene="murC"
                     /locus_tag="P5541_00805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRV70542.1"
                     /translation="MNDLTGRFEELKNVHLMGIGGAGMSGLALLLRQFGMNISGCDVS
                     HTHYIHKIATQGIKFSLGHDKNHLDVYKPEAVIYSSAIPSDTEELLEARRKGILTFKR
                     AEVLSWLFNMRKGIGIAGTHGKTTTASMTGLILENAGLDPTVAIGGELCDIGGNAKLG
                     SGAHMVAELDESDGSFELFRSHVAVVTNADWDHVDFYPNFNSVLKAYKRFLENRAEEG
                     TAIICGEDKGLSILLQNNIQGKYLTYGWGNGWDWGARDVRHTPGGGVSYTVCRKGSPV
                     CDIVLQVSGEHNVLNSLAACAAADSAGIPLELSAQALRSFRGAKRRLQHMGFINRMNV
                     DIYDDYGHHPREVSATLSTLKKMFPDRRLTAVFQPHRFTRTAAMYREFAEVLSLADGL
                     FLLPVFPADEIPIEGVSSTLIGDLVMQKGHGEYALCNDMDEVVNRICSSVKEGDVITT
                     IGAGDVSIVSEKIMQRLERKGVTLDAVAVKA"
     gene            44821..45681
                     /gene="murB"
                     /locus_tag="P5541_00810"
     CDS             44821..45681
                     /gene="murB"
                     /locus_tag="P5541_00810"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRV70543.1"
                     /translation="MTTLGVGGPAECLARPKTLGEFCLFLDFCRSEGTPLWIIGSGSN
                     ILVPDDGLPGITLQTFGLNGIFWEEQGDFVLLYAESGVLMASLLGLSIRKGWSGLEFS
                     AGIPGTLGGALSGNAGVGDLSIGGLVESVGVVDTNGKVETRQAGDCIFSYRYSSIGEE
                     GTPIIFCTLRLERSSPDHVADGTRKMLSRRKGQPKGARTAGCIFKNPPGQSAGMLLDE
                     AGCKGMSCGGATISRRHANFIENSGSATAGDVASLIVKCADRVRGRTGISLQREIRFL
                     GYIPHEKINY"
     gene            45848..46444
                     /locus_tag="P5541_00815"
     CDS             45848..46444
                     /locus_tag="P5541_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70544.1"
                     /translation="MIRSSKIEKSLMSVAPVSVDIEMTSPGFFKVDFLPLEPWFLVFW
                     SGGEWFLSREGRMWSVHHSLNNIISGQEAIEGPVLVWGEDLSDPVPAGMDFENSVVDS
                     SLPMGEILSWKDALVKTGFYPRVSSVTVRKREGRRIIEILERTGSGSIRILLADTTEN
                     WKTLFDAVDEILSQPGVSGKNLVLDTTYTGRILVRVIS"
     gene            46477..47838
                     /gene="ftsA"
                     /locus_tag="P5541_00820"
     CDS             46477..47838
                     /gene="ftsA"
                     /locus_tag="P5541_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRV70545.1"
                     /translation="MRPEPADPFLNSVLDIGTRKIAVAVFESDPATMEFHVNCIATAH
                     SMGIRKGDILDPDLVVESIRQAFEKAEKEVGLHIRDTVVSFGPTGTRTIVSEYKICLS
                     DETEPEKPVNASDMKEVVAKAIASECPRPEEFLMHAFPMEYSVDGGQPLKDPRGVKGT
                     ELSVSVLCIFVPEQSFLDALDCVRKSGVHVHGIIHKAVSSALGLLSNEEFRRGAVVVD
                     MGAGTTSITTIRDSRIRGVSLLPTGGDHVTGDVAEVLGIPSFAAEFLKREVSLEEEED
                     SLSDELEIEIDGKPFVTTVEEVLYIVSSRFEEMMVRFVKPGILSMSAGKGPMEVLFSG
                     GVTRSPGFIDMTENLTGFRCRIAEPVNSRSLPPGKGGPEFNSLIGSIRYISEREESPL
                     DYLEPSFEAYNEDPDDRSQIDRDTSAIFGSRVKPFRSGKRAKKNRLSGLADAVRKAFR
                     ELF"
     gene            47865..48995
                     /gene="ftsZ"
                     /locus_tag="P5541_00825"
     CDS             47865..48995
                     /gene="ftsZ"
                     /locus_tag="P5541_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRV70546.1"
                     /translation="MPELFEIEGSQRQIQEVIKVVGIGGGGGNALNHIVRSGVRGVEF
                     IAANTDIAHLSLSEADLKIVLGRELTRGLGAGADPEIGLRAAEESVEEIRTILEGTDM
                     VFLTAGMGGGTGTGATPVIAKIARESGALVVSVATKPFNFEGKKRISQALEGLENLRD
                     HVDALIVIPNDRLLEISPKGTTLSDAFKLADEVLRQAVQGVTDLILRPGLVNVDFADV
                     RTIMTQAGTAIMGIGEGKGEDRASKAAHLAINSPLMDGPITGARGVLFNVTGGPNVGI
                     HEIQEAAEVVTRAADSQATILWGHVLDPDMDEKIQITVIATGFSPEGMRKKARTFMDA
                     PIREDTLADFQSASGDDKTLACDPFEDLDRPVVKRKPVSRNE"
     gene            49019..50548
                     /locus_tag="P5541_00830"
     CDS             49019..50548
                     /locus_tag="P5541_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV70547.1"
                     /translation="MSSRRGKTGRAYGFAGARYGEVILDNVPSGGSPSWAMLYPAEYA
                     VGMANLGFQSICAELRTMGVGVERFFQEKGTPRSVESCRSINNFPFVSASIAYEPDLV
                     SIINIFSQWGISPNWDERESRGDPVFGVGGALSYINPLIFSGIADYVVLGDGEPVLEY
                     LVGECRKYMSHGDRHRLWEKLSEHPSIFVPPLHRDRILSGKSTGRKKSFVSDMDSVRG
                     KSLWVSGDSVFGKTILVELQRGCRRGCSYCTIPSSFGPVRIRSLEKVTGDIAGFKDSG
                     NLQVGLVTPEAGDYPHIRQLLEYLRSAGQAVSFASLRVDNMTEEMIEALVRSGRYSVT
                     VAPEAGKDWLRFSCGKNFSNDLIIERLSMARRVGVRQVKLYFMTGLPGEEMEDVDAIP
                     ELCREIHLQTGLKVRASIGLFVPKPMSRWGMEPICSPEAVKARFRRLRTLFSKDHSRG
                     YLASFQDENESFLEHSISWFGAGNGSFPGGLNRRAMRAIIKEYVTDKESVREQLENLG
                     F"
     gene            50580..51197
                     /locus_tag="P5541_00835"
     CDS             50580..51197
                     /locus_tag="P5541_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRV70548.1"
                     /translation="MSFRKTRKFKEKPAIFAFGHIMLPLVGILALGLLVLGVRLLFVN
                     PGGQISYPEPSRPQASQPDPEPEPEIVVADQQSEIVAVPVVEDTDSSPSVSLKQDNEV
                     AKASKPSQTPATKPTATNPLPSTDVRAGTWVVQIGAFTLKESAEALAKEARGKKYEAF
                     VFQAEVGGKTYYRVRVGAGSQRTDAETLARDLASKGYPTLVTRHE"
     gene            51243..52487
                     /locus_tag="P5541_00840"
     CDS             51243..52487
                     /locus_tag="P5541_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRV70549.1"
                     /translation="MSGFVTRTFSRGEAIEKIAGLMGFPWVFELSEPIPLEKAIGYPS
                     GEDLISETPNPPFNRSLRDGYAVCSEDLTGTSESSPVFLTLCGEVPMGKLPEFNISRG
                     CAALVHTGGAIPEGADAVVMIEDTSESGSAVEIRKSIQSGENVLIEGEEFLTGQVIVE
                     KGEIFNFRNIGSVASCGFAKVRLVDLKIAVISTGDEIVPVGIPELLPGMIRDSNSSVI
                     MAHLLRAGFRSRFMGIAGDDPGRLGDLFHEALAGNDVVLLSGGSSVSVRDHSVELLRT
                     LGEFEGDVPVRGLNISPGKPTIVSGNASKKKIAVCLPGHPHSCSVICATFLVPLLRSA
                     VRGKQEELFKKIRLPAASDIIGKSGVEEFIPARIDSNGEALPLWGKSGYVMALNSSDG
                     FIRLSEQEETVRCGKKVEVWLW"
     gene            52454..54391
                     /locus_tag="P5541_00845"
     CDS             52454..54391
                     /locus_tag="P5541_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV70550.1"
                     /translation="MRQESRGVALVVFPENISLEKAWSIIEERVRSLERGEMVEIADV
                     GAPYGRILASDLFARFNLPHYPASAVDGYAIKAVDTARASSTSPSRMEPGSYIWVNTG
                     GGVERKYDAVVMIEDTFMDGEFLMVTKMVSQGDNVRPEGEDVAMGQVIARKGEALNPL
                     NLPLLIASGYSSVPVQPLPRTIFLPTGDEIVEIARWINGGYGESDQAPETNSWMLRGL
                     FSEWGFPLDVGEILPDDPGIIGGAVEKALGDYDLVVIGAGTAKGRRDHSAGIISRFGE
                     PLFRGIRMKPGRPVIAGISGNRPLVALPGFPMSALVTAWTILYPMLCRLSGQAFHTNI
                     QKAIGCHKTFETSLLRHHSSQQGIREWMRVKCGEIDGTLYSWPLASGASSMFSISDAD
                     GVALLDESTLELPKGSRLNVWMRNDPDWKRRIVYQGSNDPAIEILVSHMRDHGGDMAI
                     RSVGSLGGLAAISRGEGHVASCHLLNPEDGTYNSAYINGFSGSGDWVRIRLFRREQGL
                     IVYRGNPLGIGSVKDLSITGAFFVNRQPGAGTRVLFDHLLKENDIVSGKIKGYNSIAV
                     THLEASARVGSGSADATLGIRAAADAFGNDFVPVTMEDFELIIPGRFMNHDGISILIE
                     TLDDANWRLEVENLGGYSWII"
     gene            54388..55356
                     /gene="moaA"
                     /locus_tag="P5541_00850"
     CDS             54388..55356
                     /gene="moaA"
                     /locus_tag="P5541_00850"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRV70551.1"
                     /translation="MKDKFGRRLNYIRISVTDRCNYRCVYCMPEEGLEWIPHDRIMTY
                     EEIVFLSSVLHGLGVEKIRFTGGEPLVRKGFFDFLKDLADKVPDIRTVLTTNGSFIGE
                     YIEEIRESGLSGINVSLDSLNPAVFGMITRKGPLECVLKGIRTLSQETDIPIKINTVL
                     MKGVNDEEIPELLDFAMENRATLRLIEFMPLDDSVWREDLFIPASSIFEKLPRPGSWI
                     RIDNSSDHSAGPAVYYKNEATGQKIGIIEAVTQHFCEKCNRLRISATGILRPCLFSED
                     GIDLRPSLISRDPALVRKNLMQGALDKPECYAASKTGKMHMSRIGG"
     gene            55358..55843
                     /gene="moaC"
                     /locus_tag="P5541_00855"
     CDS             55358..55843
                     /gene="moaC"
                     /locus_tag="P5541_00855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRV70552.1"
                     /translation="MGQFSHMDDYGHPVMVDVGGKPHTIREAVAEGLVRLPVKVMEAI
                     MTGAAVKGDVLRVAEMAGIMGCKRTPDLIPLCHPIKLDHAAVECSVDPEKRLARIICR
                     TRSCDVTGVEMEALTGVSVAALTLYDMCKGIDKGMVIENIRLLRKDGGKSGSYIAEDL
                     K"
     gene            55840..56649
                     /locus_tag="P5541_00860"
     CDS             55840..56649
                     /locus_tag="P5541_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRV70553.1"
                     /translation="MKSRVMGFSGSLPAQPVVFLHEDSERRPLVNGKALKITILEPGQ
                     KKSGDDFFVIRASGFGEVGPGDFVSFSEGVILRMDPAIHDGSMFVVRPGFISVSSTLE
                     VWSSIQAGVLTVSDKGSRGERIDTSGPALEAALLGIGVSTLMREIVPDDAEKISRILG
                     EWISSGKIPLVLVTGGTGLSPRDVTPEALLSLGGKIVPGLGEFMRWKTSFLNERSILS
                     RAFAVACEKTLVISLPGSEKGAIECFSSISSVLRHALEILSGKGTNCGSSH"
     gene            56680..57564
                     /gene="rapZ"
                     /locus_tag="P5541_00865"
     CDS             56680..57564
                     /gene="rapZ"
                     /locus_tag="P5541_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRV70554.1"
                     /translation="MKESLANRVSRCMIISGMSGGGKSTALHMLEDVGFYAIENIPPN
                     LLPQLIQVLGKHEEAVQNGVAAVVDVRGESLLDHLFSVIGYLKKKGLEVGVIFLDASD
                     STLVKRFEETRRRHPLSCDSTILEAIQMERVELSKIHEIADVVVDTSNMSLAELRREI
                     LGKIEISEGPLSVIFTSFGFKHGIPPDSDFVFDVRFLPNPFYEPSLRSGSGKDRKIQE
                     FIARSPDISAFIENLSSLLGCIFGLYMNTGKTQVHIAIGCTGGRHRSVAVAEWLGEKF
                     PENSLVRHRDIEKDTVLQ"
     gene            57561..58694
                     /locus_tag="P5541_00870"
     CDS             57561..58694
                     /locus_tag="P5541_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRV70555.1"
                     /translation="MNWWSAFTVGLLSGGIMSLLAARLFQEEEEDNSSPQGKNGTVMH
                     KAFRHKVSQGPEIVAIGGGTGLSTLLSGLKSYTRNITAIVTVTDEGGSSGRLRGEWGV
                     LPPGDIRNCMVALAENDSSLNRILNFRFDKGELEGHSLGNLILLAATEMTGDFRKAVE
                     ELNNLLAIRGKVIPVTTESVTLCGSTGDGRQLRGELNISSCGNRLDSIWLEPGDARAV
                     PETLKAISEADVVILGPGSLFTSIVPNLLFSDIAVALRRCSAPKVYIANLMTQPGETE
                     GMNIIDHVRWIIRISGITPDYLIASDSPIPYEMLDSYRASGAQPLVLEEEHKNALGLM
                     GTRVVVSDYLSISEGSVRHNSQKLSETLVRIAREERDTAKWRN"
     gene            58682..59596
                     /gene="whiA"
                     /locus_tag="P5541_00875"
     CDS             58682..59596
                     /gene="whiA"
                     /locus_tag="P5541_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRV70556.1"
                     /translation="MEKLKSVVWDEWGTVPNSGREGATMEIAGILSGLPSKTENKVTS
                     FFSSRLFVFRRIGRLWDSSEWSAFGEFSSGLVIPPDLKGRVSIRIKSPGSLDLMFPFK
                     KGNRSTPDSWNWLRGVFGSSGSLFLPRTGYYMVFRLPNSSEEDLGKDIATIMRYRDIP
                     SRTRSRYGFREVAVRDQSSIVGMLSNMRLFKTSLILEEKAMLRSLRDRANKIVNCDAS
                     NIRKTIETAERQIVTARQLIDSGKMARLPEPLRDLIEARIENPTATLKELGGQLARPV
                     SKSTVEYRWKKIEKIAASAGVKIQPTGG"
     gene            59593..61551
                     /locus_tag="P5541_00880"
     CDS             59593..61551
                     /locus_tag="P5541_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRV70557.1"
                     /translation="MIVMYLGKADVNTYEKGSSREFLTTNGWGGYGFSTVIGANTRRE
                     HGLLVVNKSGKEVYPDVLISKLDETVISRGKKYHLSTNRYTDVVFPDGYRYLQEYHAE
                     PLPGMLFVIHSVFLRKTVFMPRKRLSTIIKYELLSAPEKIELEIRPLAAHRGIDQVCS
                     HKDRDFSSSFSGNCVSIEGSDLQSRFYHFNGEWRPKPLWFENLIYEFDECEKPGTDNL
                     WSPGVLSVDMSEGDTIYIVLSSGVEKYDHGICSELEDITVRWIASNVSETPLMKKTPM
                     ARDLIQSASHCISEIEGGNPVIFTGYPSVREKARETFISIPGLLLSTGKIEFARNLLE
                     VWLDRAAGNGNVMPSGIDPHTGRLTSQSADSGLWFFYALGKYCDRSESTALAEKRWGD
                     ITALALKIIEGIPEIGLSLDKDGLLDLRSESIGSNWMDGSNNGTAIVERHGKLVEFNA
                     LWYNGLRTLEQLAELTGRADQARDFGKMADLCGTSFKKVFWNEKGYLYDWVDGDKKSD
                     SNRCNQILAASLPVSVMDPERSRSIVESCWNDLYTTFGLRTLDPHHEKYKGRCEGRLD
                     QREKARFMGMAWPWMLGQFITAFLRYNPDRKDIAWCFLRPFLSHLRKGCLGGVAEVFD
                     GSMPYRPHGNALYAPSVAELLRVISEDM"
     gene            61687..62700
                     /gene="gap"
                     /locus_tag="P5541_00885"
     CDS             61687..62700
                     /gene="gap"
                     /locus_tag="P5541_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRV70558.1"
                     /translation="MQKKRVAINGFGRIGRLALRALYEYENEGLFDIVATNSRTSAEQ
                     RAYLFKYDSVHRRFSGDLKISEGSLVINGKEIKVLAEPEPKNLPWKELGVDLVIESSG
                     KYTDARMAEGHLSAGAKKVLITAPAKNEDATIVMGVNEHIYDPSAHHIVSNASCTTNC
                     LAPVVKVLYENFGIEEGLMTTTHSYTNDQKTLDSSHKNFHRGRAAALSLIPTTTGAAA
                     AIGKVYPQVEGKMNGIAVRVPTPDVSLVDLVVRLSSPATKETINGAFKKASSGPLEKY
                     LGYEEDDLVSVDFTGDSRSSIFAARHTMALGTMAKILAWYDNEWGYACRVIDLANYML
                     GKE"
     gene            62713..63903
                     /locus_tag="P5541_00890"
     CDS             62713..63903
                     /locus_tag="P5541_00890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRV70559.1"
                     /translation="MALRTFEGVPLEGLKVLVRVDFNVPMKNGKIKDDSRIIAHLETI
                     TALKNSGACVVLISHLGRPNGKADPAFSLRPVAEALSIEYGIASGFVADCVGREVASS
                     VKAMRPRDVLLLENARFHPEEESNDPSFAKKLAEPFDCFVMDAFSASHRAHASTEGVT
                     AWLPSYAGRVMEKEVSVLSTVRENPTKPLVLVMGGAKVSDKIRIIKYMIDRTDSILVG
                     GAMAFPFLKALGKNIGSSFCEEGTEETALEILEMAEKAGARINLPVDVIVSDSIENSR
                     NSTCVSLSSIPNNMMGLDIGPKTAEQFSVVISGAKTVLWNGPMGVFETPPFGSGTKTL
                     GESVAKATSKGTLSVLGGGDTAAAAKALGFSDGISHISTGGGATLEFFEGRDLPGIKP
                     LETA"
     gene            63932..64723
                     /gene="tpiA"
                     /locus_tag="P5541_00895"
     CDS             63932..64723
                     /gene="tpiA"
                     /locus_tag="P5541_00895"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRV70560.1"
                     /translation="MKEQILLAGNWKMNKDIKGSLCFIEEFQEMAEKEDLFGKTGKKF
                     EMAIFPQTVNLYALNQKILDLKLPILLGAQNIHWERSGALTGENSGSSVLEAGAKMAL
                     VGHSERRHIFGETDEMTARKYVSCVECGLRPVLCVGETEEEKSRGFTEKVLSRQLRSV
                     FAPDRSSEEWGEIIIAYEPVWAIGTGKSATSEDAAGSCDFIRNLLKGICSEKESQNAM
                     ILYGGSVKSSNAAAFMENGSINGLLVGGASLDPFEFMGILRACGV"
     gene            complement(64795..65151)
                     /gene="rsfS"
                     /locus_tag="P5541_00900"
     CDS             complement(64795..65151)
                     /gene="rsfS"
                     /locus_tag="P5541_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRV70561.1"
                     /translation="MKASIKGDSVAPIVQILQDKQAEDITIIDLSRESTVADAYVLAT
                     GNSDVHMKALLGHVTDTLDSMNVGYRIEGENSPKWILLDAGDIIVNIFGREGRDFYRL
                     ESIWASARTIKANQTV"
     gene            complement(65164..66420)
                     /locus_tag="P5541_00905"
     CDS             complement(65164..66420)
                     /locus_tag="P5541_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRV70562.1"
                     /translation="MKRLTRKNMALIALACFVSLVAGVGLKAYFFFNPAPNTIREKIQ
                     HDEVTGRINMVFVGLDEVPGETANRSDSIAFVSIDIDEKIVRLLSLPRDTRVQIPGHG
                     WQKINHAYAYGGVELLKATLVNYLGVPIPYHVIVNFESFPKLVDMIGGVTLDIPKRLK
                     YSDRAGNLFIDIPAGVQHMDGKTALGYVRFRHDALGDIGRVERQQIFLKAAFARIKEP
                     GMVTRIPELIKYSMKIVKTNMSLSQSIQLASYLKDMDPSRTVFRTLPGKAAFISGISY
                     WLGDPGTLSEFFSEIPEENPAGEQTDTEDPALISELVIEIGLPVTVLNGSGTAGIGKE
                     AATRFQSVGIDVGHIGNARHYDYRHTNILYPPGENNQKAAEALGKLTGIQQNLVKKDT
                     SISRVTIIMGHDYMDVLKRFNSSSAR"
     gene            complement(66417..67031)
                     /gene="nadD"
                     /locus_tag="P5541_00910"
     CDS             complement(66417..67031)
                     /gene="nadD"
                     /locus_tag="P5541_00910"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRV70563.1"
                     /translation="MRKRKIGVMGGTFDPIHFGHLIAAEEAYFSLGLTEVVFVPTGDS
                     FHKAGTVISSAEHRYTMTMLATLDNPHFRISRVEIDRETPSHTVDTLREMRHWYPPKS
                     VEFYFITGLDAVLDLMNWKEPLEIPKLCKIVAVTRPGSDPGEIARLPEEIRENMINLE
                     IPLLAISSTEIRKRVSDGLNIRYLAPWLVEQYIYKNAMYFRNGW"
     gene            complement(67065..68393)
                     /gene="obgE"
                     /locus_tag="P5541_00915"
     CDS             complement(67065..68393)
                     /gene="obgE"
                     /locus_tag="P5541_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRV70564.1"
                     /translation="MRFIDVAEIWVESGKGGNGSLSFRREKFVPKGGPDGGDGGRGGN
                     VYLVADDRIQTLADFEYRRRFAAGDGSHGKGGNRHGASGEDVKVPVPCGSLVIDPGTG
                     TIFADLTVHGDTFLAARGGRGGRGNARFASSRRRIPRFSENGADGEKRNFLLELKMLA
                     DVGLVGLPNAGKSSLLSAISGARPDIAGYPFTTLSPNLGILAVESEKIVIADVPGLIE
                     GAHLDRGLGHAFLRHIERTRLIVYVMDLSEGASAPPLEQWNTVRNEFLEYNPELLSFP
                     SAAVGNKTDLPRDKDGYSALREELYKAEIPFFETSAVTGEGIEAFIGHIVSVIHKVPA
                     TKRESRFFEIESPFTEDLKKIKPVIKNPGNDGLFQVFHPEIERLIKRFNFDQDEALGR
                     FMALMQHYRIEDLLVKHGAREGDTIIIGDLEFTFIPESPPNQEKASSEEG"
     gene            complement(68475..68756)
                     /gene="rpmA"
                     /locus_tag="P5541_00920"
     CDS             complement(68475..68756)
                     /gene="rpmA"
                     /locus_tag="P5541_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRV70565.1"
                     /translation="MKIFDLQLFAHKKGQGSSTNGRDSIGKRLGIKRSDGQEVTAGSI
                     LVRQRGTKYHPGDNVGRGRDDTLFALKPGKVRFVTKAGRKFVAIENVIA"
     gene            complement(68743..69081)
                     /locus_tag="P5541_00925"
     CDS             complement(68743..69081)
                     /locus_tag="P5541_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRV70566.1"
                     /translation="MTEIRILRCGDIICGIEISGHSGYAGYGKDIVCAALSTLAQTLE
                     IGLTDVLDIDNVVSTKDPVSGYARIFWGNNDSSRINDLAKTIVAALVSISKSYPAYTK
                     IVEVYVNENF"
     gene            complement(69089..69403)
                     /gene="rplU"
                     /locus_tag="P5541_00930"
     CDS             complement(69089..69403)
                     /gene="rplU"
                     /locus_tag="P5541_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRV70567.1"
                     /translation="MYAVIETGGKQYRVSPGDRIRIEKIDVDEGGAIEFNKVLMVTSD
                     HKVSIGSPTVEGAVVKARVETHGRDKKIIVFKYKNKSKQSRKTQGHRQSFTSVLIDSI
                     EY"
     gene            complement(69566..71218)
                     /locus_tag="P5541_00935"
     CDS             complement(69566..71218)
                     /locus_tag="P5541_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV70568.1"
                     /translation="MLSELIVSNIGGIKDATLNFRGNFIAITGESGAGKSSLVRALEL
                     ASGKRAQSAQIRAGVDEAEVQAVLEMDGPVPSLPDDLQPQEGFMVIRRLVSSTGRARS
                     YLQERPVPLNIVNSALSNVIAIQSQFAQLGLLEPSRQVEMLDNSGGPELKEIRSRLSR
                     TVALALETERKLIEINRRRRETEAACQDGESFLARYRNLKLEESSFSEWETTMNDIST
                     SLRNQKQMKSIHDRLTGGSSGEGILSSLESICRESVIAAPSDEPLYGASENLLRAAQE
                     FEDVIKTIVCRSNEQELQNHFDDLETRLGMLRKLMRFAKTDNVNDLVEYAERTKAGLD
                     WLKESNQMLAKLQEESTSYKKEVSSLALSLREMRRKAALNLEEAVNSTLRNLAMEGLR
                     FGISLNPLEKVRQNGADEVSFFLTDDKHLYGPVAKTASGGELSRILLSLQIASSDEML
                     PDVLVFDEVEAGLGGRAAVLAGYALKELSKRCQVLLITHEASIAALADQHFKVEREGD
                     TSRVMEVDHDSRVLEISRMLAGNPDDPEASRLASSLLGARNN"
     gene            complement(71223..72098)
                     /locus_tag="P5541_00940"
     CDS             complement(71223..72098)
                     /locus_tag="P5541_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRV70569.1"
                     /translation="MTTLGLLVNTQKRPALDLAEKILRWGRKRNVNFALPSHEAHILG
                     VEEISDKDWKERVDAAVVLGGDGTFLRAARYVIGYDIPLYGINMGHLGFLSCGKAENA
                     EKDLEIIIQGKHNIQYRRLLHGVVSRGKRQKHEVYALNDLVVTKGPFARLIALNIAVS
                     GCPLSVLHADGIIISTPTGSTAYSLSAGGPIIPPHLSCMILTPICAHTLYSRPMVLSD
                     SDTISITPEGQHREIMLTQDGQLGYELLPGDRITVTLDPSRRVGIIALEGNDFFSLLQ
                     EKFQWGHRIAESDEE"
     gene            complement(72126..72950)
                     /locus_tag="P5541_00945"
     CDS             complement(72126..72950)
                     /locus_tag="P5541_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRV70570.1"
                     /translation="MPSPQKERLDLEMVKRGLTESRTRAQSMIMAGDVTVDGQKTTTA
                     GLRVSDDSDIRVKDGPVWASRGAHKLLKGLKVFGIDPLGRVCADIGASTGGFTDVLLS
                     LGAKTVYAVDVGYGQLAWKLRQDPRVVVMERTNARNLEAGDLKPPPDLVSVDVSFISL
                     RLVIPAVERVIDQGGECVCLVKPQFEAGRSAVGKKGVVRSFESHVAVLVSILDFLENN
                     SSLFYAGADYSPVLGPKGNIEFLIHLKSTSAGLLTSRGNEQIKDMVKEAHSKLLGP"
     gene            complement(72841..74799)
                     /gene="dxs"
                     /locus_tag="P5541_00950"
     CDS             complement(72841..74799)
                     /gene="dxs"
                     /locus_tag="P5541_00950"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRV70571.1"
                     /translation="MREILERIRDYRDIADLENNQLDKLCLEIRELIIETVSENGGHL
                     GSSLGAVELIVSLLRSFDPARDKIIFDVGHQAYAYKILTGRKNRFPSLRKWGGISGFP
                     DPKESVFDHFNAGHSSKSLSASLGFAKARDILGQDHHVVAVIGDGSLMNGMALEALNN
                     THEAGTRVIYVLNDNQMSISPRVGGMANHLAALSVSPFYHNLKSFLKYFCRKLPRGNR
                     LESFLEKIKQKIKGFLLTPNMFEEIGISYWGPFDGHNIKEMQRIFDLARSYEFPLLIH
                     ILTKKGKGFEPAENMPETYHGVSPKIQVSVNDRPKSLCWSSASASCIEDMAKKDPRIV
                     CMTAAMKEGSKLNRFSSIYPERFFDVGIAEGHLLTFAAGTASAGLLPVVSIYSTFLQR
                     AMDQLVHDICMQNLPVILAIDRSGLVGEDGETHHGILDVSWCRSIPNLTVMAPRDIPD
                     LRAMYEGAAKKMSPCAIRLPRGIAPEAEYRNRSHVTAPWGKGEKLVEGSGWALCAYGS
                     TLPLARITREKAISKGLLPPSVYDMRFLKPLDIDMIREMLSAHNYVIVLEDGYREGGI
                     GEKISAIATEGPFRSMVITMGVPDEFVPHGTVGEQWKHCGLDPEEVIGKYHAITSERT
                     AGPGNGQKRPHGESDSRAVNDNGWGCDR"
     gene            complement(74828..75733)
                     /locus_tag="P5541_00955"
     CDS             complement(74828..75733)
                     /locus_tag="P5541_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRV70572.1"
                     /translation="MEKNRFEKILSPYREQVDSALVELSGSRPENIPLRLWESMTYSL
                     LAGGKRIRPILCLLSAGACGVSKNDAMVMALACEMVHTASLIHDDLPAMDNDILRRGK
                     PTNHVIFGEPLSLIAGDALFLWAFEFALEGLAKRNKFQPETIMAAVSELLKAAGPKGI
                     CGGQVLDSDPESTLSTEEHPWRVAFSKTASLIRASVLSGVVLGETDEETRSAFSAYGE
                     HLGMAFQIVDDIIDVTGSKSELGKTPGKDSEQGKITFVAAFGMEKARQLAARETSLAS
                     RAISRVNGETDLLASLPGTLLDRSI"
     gene            complement(75740..75985)
                     /gene="xseB"
                     /locus_tag="P5541_00960"
     CDS             complement(75740..75985)
                     /gene="xseB"
                     /locus_tag="P5541_00960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRV70573.1"
                     /translation="MTFSDDIAELKDIVENLDAGDMPLEESLALFERGVALISSCKKF
                     LESARQRVTLLSENIADTESSPWNPLEDDREEEVEGS"
     gene            76116..76310
                     /gene="rpmB"
                     /locus_tag="P5541_00965"
     CDS             76116..76310
                     /gene="rpmB"
                     /locus_tag="P5541_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRV70574.1"
                     /translation="MAKVCDCCGRGQATGNNVSHSNRHTRRTWNINLQSVKVDLGCGE
                     SRKLKICARCLRSGKVKRAV"
     gene            complement(76324..77034)
                     /locus_tag="P5541_00970"
     CDS             complement(76324..77034)
                     /locus_tag="P5541_00970"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRV70575.1"
                     /translation="MGTTLELPQFKAVFSKGEGNFPLVLVAGGRQPEPWWFQDLCQSR
                     RVWAIDSGIDLCRKNFVLPDLFIGDRDSASAEGILWAESKKIESMTFPPEKNLTDLQI
                     ALREKGLRWPGTPAILTAGFGGRFDHLFSNIFSLIWGGEEWRSFTRCIADDKEALLLL
                     RGGEDVGVSGLETGTLISLLALSDRCLGVCLEGTRWPLEEAVILKNRPYAVSNYSVTG
                     NLRLGIDEGWIGLYVRKP"
     gene            complement(77039..>78435)
                     /locus_tag="P5541_00975"
     CDS             complement(77039..>78435)
                     /locus_tag="P5541_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRV70576.1"
                     /translation="DYFFDLDRDLLESRGESIIATLDEFGIGAELSETVVGPTVIQYR
                     IQLAPGIKVSRVSGLANDLAVSLALPSLRVEAPIPGKTCIGIETPNPERKPVNLRTVL
                     EHPSFLESDNDLAIPVGVGVDGRHLSIGLEKMPHLLVAGTTGSGKSVFVTCCIAALCS
                     RNSPEDLRLLLVDPKRVEMKSFEKLPHNIIPPVVESRQAVHALGWAIREMEKRYEMFA
                     QARVRNIKTYNTRVLPVDRMPYIVIVIDELADLMFTSPREVEDYICRLAQMARATGIH
                     LIIATQRPSVNVVTGLIKANIPARVAFTLPSQTDSRTIIDVAGAERLLGSGDMLFLTP
                     RLPKPLRLQSPWIDEPTIQRFMDYLSRLFGDPVFLDIDVQGDDGGENGRVHLDDDLLE
                     EAMNMILHSGIASASRLQRQLRIGFTRAARLIDTLEQIGIVGPQDGSKPREILVDEDE
                     ARNILFESMNGNRG"
BASE COUNT        19678 a        17995 c        21407 g        19355 t
ORIGIN      
        1 aggagacgga gtacgtcgaa tcgataaagg aagatgttgc ctggctgggg ttccggtggg
       61 caaacctcag atttgcttcc gactattttg atgaattcta tgtgtgggcc cttgaacttg
      121 ttcgcgccgg gaaggcctat gttgatcacc agacggcgga ggaaataaga acaaaccgcg
      181 ggaccctgac cgagccgggc aaggattcac catacaggag caggagcgtt gaagaaaacc
      241 tggaactctt cggaaaaatg aaagatggaa agatacctga cggggagtgt gtgcttaggg
      301 cgaagatcga tatgggtcac ccgaacctga acatgaggga tcccgtaatg tacaggatcc
      361 ttcaccagtc gcaccacagg accggggaca aatggtgtat ctaccctatg tacgattttg
      421 ctcacgggta cgaggatgcc atagagggag tgacccattc aatatgcacc ctggagtttg
      481 aggaccacag gcccttgtac gactggttca tcgagaacgt ttcagtcccg cacaggccgc
      541 accaatatga gtttgccaga ctggcgctca catatacggt aatgagcaag agaaaactgc
      601 tggaactggt gaacaagggt tatgtctccg gatgggatga cccgcgaatg cccacgatca
      661 gcggggtcag aagaaggggt tatacccccg catctatcag gacattctgc aaaaagatag
      721 gggtctccaa agccaacagc ctcgtggatg tggaactatt gcatcactgt atcagggatg
      781 aactgaacca gacggcccga agggtaatgg ctgttctgaa accggtaagg ctggttatag
      841 agaactaccc tgagggtcaa tttgaagaga tcgaggcgtt ggacaatccc gaagcgtcta
      901 tcgcgacttt ccggaagttg ccttttggca gggaactgtt catagaaatg gacgatttta
      961 tggaagatcc cccaaagaag ttctttcgac tttcaccggg tgccgaggtt cggctcaaac
     1021 atgcttacct ggttcgatgc aacggatttg acaaggacag tgacggtaat gtaacggaga
     1081 tacgctgcac ctacgatcct gattcccgtg ggggggaagc tcccgatgga cgcagggtca
     1141 aggggacact gcactgggtc agcgctcctc atgctgttga tgccgaggtc agactttatg
     1201 accatctctt taccctgagg gatatggact ccatggaaga gggtagagat tacaaagact
     1261 acctgaaccc ggattcgctg caaaggttga cgggctgcaa ggtcgagcct tcacttgccg
     1321 aagctggccc tatggagaga ttccagttta tgagaaacgg gtatttctgt accgacccgg
     1381 agcacaccag gaaacatcct gtttttaaca gaaccgtttc actgaaggac agctgggcaa
     1441 gggaaaaaga caggaaataa aacattttcg tcttaaaata ccggcggagg gtctccgccg
     1501 aaacgccgga ggggtcttga aaggaaaaga gcagtattcg gaaccgactg gttccggcag
     1561 cggatctgta ctgaaacgtt tcaggtttgc aggaaagaat caggagtggt gggactccgg
     1621 aaagactgtc ctggcggcgg tttcgggtgg aagcgattca atggcgatgc tttggctcct
     1681 gcggtattac tggaagggaa gagttctggt ggcccacctt gaacatggat tccggcagga
     1741 aacagctctc agggatgcct gcttcgtcga gaatatctgc cgggattggg gtattgattg
     1801 ccgggtcgag catcgtaacg ttccctctct caaaaaaccg ggagagaccc tggaagaggc
     1861 cggcagacgc gagcgttatg attttctcct tagggtcgcc agtgaagcag gggccgaatt
     1921 tgtcgcaacg ggtcatacag ccgacgattc aacagaaact gtttttttta acctcctgcg
     1981 tgggaccggg atcagggggc ttcggggtat acccgaggtt cggggaaata tagtacgacc
     2041 ggtcattcac tgtttcagag aggaactcca gtctttcctt tcggaaagat ccgttcaatg
     2101 ggtcgccgac gaatcaaacg atgatgtatc ctattttcgc aacaggataa gacatgtcat
     2161 tttccctttt attcataagg aggggaatcc ccgtttcagc gaacatcttc tggccctgag
     2221 cgaagaagtt tccgaagcgg agaaggtccg ggacaaaaga tcagtatcgc ttgcggcgtg
     2281 ggcacgaagg ccctttccgc tggcgatcag ggtctgggac agaaagaccc tgttaagaat
     2341 gtgcaagggg gatcttcagt cactttttgc ttttgaggga agatctctgg ccctttcccc
     2401 actttccagg gtcaagaccg ccagactgct ggaactggta caagggtcag aaaacccctg
     2461 gcgttttcaa tgggaaagag acctagagat ctgtggttcc gggaaaattg tcgctctcgt
     2521 taaaagggct ctggccgagg gggcctctcc ccctgagaaa atttttggtt ttcccgaggt
     2581 tgcgggttct atccgctggg gtccctggaa gcttgaatgg tttcccgtct cgggagaagc
     2641 aacaccttca gacggagact tttcgtgtat aatgccggtc tctaaaggga gtcttctcaa
     2701 ggtcaggagc atgaaatgtc ttgataaaac ttgcaacagg aaggccaggg tcccatggtg
     2761 gtgtaccggc acctggcctg tggttcagtc cggtgggtac ttgtggtcac cgattgaagg
     2821 tttcatcgga aaaccggaag taacttctgc ggccctcttg ttccgtttca gggtttgcga
     2881 gaccagggac ctcaggaaag gggatcatgg acatgactta tgtgctgggt gatgttcttt
     2941 taggcaggaa ggatatcgac cagaaagtca gggagatggc tctggacatc tgtaccgcct
     3001 acggggagaa ggatctgatc gtggtggggg tgctcaaggg ggccgttgta tttctttcgg
     3061 acctgataag gtgcttccct gtctcaatgg ctacggatat cgattttctt gctgtttcct
     3121 cctatgggga ttcaaccgaa tcaagcggtg tcgtcagaat aaccaaagac cttgacatca
     3181 acattacagg aaagaacgtt cttgttgtgg aggatattgt tgatacgggc cttaccctgt
     3241 cctatctcat gaatattctt cgcgaacggg agcccgagat gcttgacgta tgcgctcttc
     3301 tcgacaaacc atcccgaagg aagaccgata tcgagataac cttcaagggc ttcgaaatac
     3361 ctgacgaatt tgtggtaggc tatggcctgg attattcggg caggtggcgc aaccttcccg
     3421 aaatatacac cctcaacgag gagtagcgtg acagggctcg cagagacggg tcattagaac
     3481 gagaggtggg actgtattgg gaaaaatgac taaaaatctt gggctgtacc tggtgatgat
     3541 cgttcttgtg gtcagcctgg tcaacatgtt tctttctcct gcccagggtc cagaggagtt
     3601 tgaatcgata agctacagcg cctttctgaa ttatttcagg gaggggaaac tggagcaggt
     3661 ttcgatagag ggggatacca tcaagggcac ccgggaaggg ggagtcaagt tctcaaccgt
     3721 tgctgtcggg attggggatc tggctccgca actggccgag aaaggggtcc aggtaacggt
     3781 tctgcctccg gcccggtccc catggtgggt gaccatgatg tcatcgcttt tccccacgct
     3841 tctgctgata ggggcatgga tatttattct ttatcacatg cagggtggaa gcaacaaggt
     3901 gatgaatttt gccaaaaaca aggcaaagtt gttcctggac aacaggccca aggttacctt
     3961 cgaagatgtt gcaggatgcg atgagtcaaa agaagaactc aaggaagttg ttgattacct
     4021 gcgtgaccct ggtcgtttct cacgcctggg agccaaaata cccaagggtg ttcttcttct
     4081 gggacctccc ggagcgggca agactctttt ggccagggcg tgctccggcg aggcagacgt
     4141 tccctttttc agcatcagtg gttctgattt tgttgaaatg ttcgtcgggg tcggtgcggc
     4201 tcgtgtaagg gatatgtttg agcaggccag aaagaaccag ccctgtattg ttttcatcga
     4261 cgagatagac gccgtcggaa ggcatcgcgg agccgggctg ggtggaggtc atgatgaaag
     4321 agagcagacc ctgaaccagt tgctggtcga gatggacgga ttcgatgttt cttcaggcat
     4381 aatcctgatc gccgcaacca accgtccgga catccttgac ccggccttgc tgcgtcccgg
     4441 gcgatttgac cgccatattg tcgtagacag gcctgacaag aatggaaggg cagagatact
     4501 caaggtccac ctgaaggaaa tgaaggttga ggaaaacgtg gatcttgatg tgcttgccaa
     4561 aaggactcca ggctttgttg gagctgatat cgcgaatctt gtcaatgagg ctgccctcct
     4621 tgcggccaga cttgacaaga aaagcatagg aatgtctgag ttcgaggaag cgatagaccg
     4681 tgtccttgca ggtccagaga ggagaactcg cctgataagc gaaagagaga aggagataat
     4741 tgccttccac gaggtcgggc atgcgctggt tgccaagaag gttcccggtt gtgaccctgt
     4801 ccacaagata tctatcatac cgagggggca caaggctctg ggctataccc tccagcttcc
     4861 cgaagaggac aggttcctga tgtccaaggt ggaactgacc aacaggatat gtgtccttct
     4921 gggaggaaga gtggccgaac agatccattt tgacgatata acaactggag cacagaacga
     4981 tctcgagagg gccacccaga tagcccgcca gatgataaca gagtttggta tgagcgaaag
     5041 ccttggcccc gtcactctgg gacgcaagca gcacgaggtc tttctgggca gggatattgt
     5101 cgaggaaaga aattacagcg acgaaattgc ctattcgata gacaaggaga tccggaagat
     5161 cgttgatgac tgctacgaaa gggtcaggaa tatacttaca gacaatcacg atgcccatgt
     5221 acggatagcc cggacccttc ttgccgaaga ggtgatcgaa ggcgacaggc tggattcgct
     5281 cctggaggaa ggaattccgg tagtgccaga ggattctgaa ggcgagaccg aagtagccgt
     5341 cgggtcatcg gcagaacagg acggcagtga accggtatag aaagaacagg ccatgtgccg
     5401 ggcaggaagc cggtgtagaa atcaagtcga gcgttcggcc agtttcaggg ccgggcgctc
     5461 tttgtcttgg aacccgtggt gggggtcgtg agcatggaac aggatagaca tcctttcaac
     5521 ctttattcgg agtggcctcc ggcaggggat cagcctgagg ctatagaaaa actggtaaac
     5581 ggattcaaca ggggggaccg ctatcagtgc ctgcttggtg ttaccgggag tggaaagacc
     5641 ttcgccatgg cgaacgtcat agaacgcctc caaaggccag ctttggttct ggcccataac
     5701 aagacactgg ccgcgcagct gtacagcgaa ttccgggagt tctttcccga aaacgccgta
     5761 agttacttcg tgagttacta tgactactac cagccggagg catatgtgcc ggtttccgat
     5821 gtgtatatag agaaggatgc ctcgatcaat gaaaggatag agaaacttcg ccttgcgaca
     5881 acgaagtctt tgctggaaag aagggatgta atagttgtgg ccagcgtttc ctgcatatac
     5941 ggtcttggca ggagaaaggc ctatgaagat gcgatactcc gtttttctgt cggtgagaga
     6001 tgggaacgcc gtgatctgat gatgaaactt gtggccaacc attacgaaag gaacgatctg
     6061 gtgctggaac ccggcaattt cagggttcgc ggagggattc tggaggtatt tccggcatac
     6121 agcgatcagg ctgtcaggat aatcctggaa gaggacctga tcgagaggat cgaggagata
     6181 gatcctcttt cgggaaagag gatcctttca aaaccgaacg ctgcaatttt tcccgcgcag
     6241 cattatataa cctctccgga ggccatagcc gaagcgcttc cagagataca gagagaactt
     6301 gaagcacagg tggccctgtt caggtcccag ggaaaactta tcgaggccca gaggctggag
     6361 atgaggacca ggtatgacat ggagatgctt tcacaggtag gttactgttc cggcatagaa
     6421 aactattcca ggattctcga cggacggaat cccggagaca ctcccggcac gctgatggat
     6481 tttttccctg aggattttct gttttttgtc gacgaatcac acatgacgat cccgcaggtt
     6541 caggggatgt tcaacggcga cagggcaagg aaggaaactc ttgtcgagca tggtttccgg
     6601 cttccatcct gtcttgacaa ccgccccctg aagtgggacg aatttcggga atttctgtgc
     6661 caggcatgtt ttgtttcggc aacgccgggg gactgggaaa tggatgtttc caccaatgtg
     6721 gcagaactga tcgcaagacc cacaggagtt gtggatccgg aggtcgaggt tgttcccgcg
     6781 acgggacagg tcgatgatct gacggaaaga ctcagatcag tcgtgtccag gggagaaaga
     6841 gccctggtta caacccttac aaaaaggtcc tccgaggatc tggccgagta cctctcggag
     6901 atcggtttcg gtgtaaagta tattcactcc gaacttgacg ctttcgagag gacggaactt
     6961 atcagggacc tcagaagggg agacatttcg gttttggtag gcgtaaacct tctgagggag
     7021 gggatagacc tgcccgaggt ttcatttgtc gggatcattg acgccgacag agagggtttc
     7081 ctgcggtcca gaagatccct catacagatc ataggaaggg cagcccgtaa tacatccgga
     7141 actgttgttc tttatgccga ccaaatgacc ggaagcatag aggccgcaat ggatgagaca
     7201 aacaggcgca ggaggaaaca gctggcccac aacctgaaac acggcattac tccggtcagc
     7261 ataaaaaaga aaattatggc cctgcttccc gaggagttgc tggaagactt gggatcagat
     7321 ctggtcacaa aacccggggg agagggtttt acaaggcagg aactggagga agttatgtgg
     7381 aaggcagttg aacgtctcga ctttgaaaag gccgccgcga taagggatat ccttcacgga
     7441 gagaaaggag gggaggtcag tcgtgccgca atggatagaa gtaaggggcg caagacagca
     7501 caatctcaaa aacatagacg tcgacattcc taagaaccgt tttgtcgtca ttacgggacc
     7561 ttccggctcg gggaaatcct cccttgcgtt tgacacgata tatgccgaag gtcagcgtcg
     7621 ctacgtggag tccctttctt catacgcgcg tcagtttctg ggagtccagg acaaaccgga
     7681 cgtggatgac atctccggtc tttccccggc gatatcgatc gagcagaagg gagtctccca
     7741 caacccccgc tcaacagtgg ggaccgtgac cgaaatatac gattatctga ggcttctttt
     7801 cgggagggcc ggtatccctg tgtgccctct ctgcggcaag cctgttgaaa agcacagtgt
     7861 cgacgagata ctggatatcc ttttccagaa gaatgaaagc aaaaaggtgg atatcctttc
     7921 acctgtggtc aggggaaaga agggtgagtt caggaacctt tttctccagt accagaaaga
     7981 ggggtacctg agggcccggg ttgatggaac agtcctttgg cttgaagagg agatccccct
     8041 cgacaagaac agaaagcatg atatagagat cctggtcgac cgcctgacag taatgccgga
     8101 gaagcgcgag aggatcgccg aagctgtgga gaatgccctt tctctcagca aaggatacgt
     8161 gcttctggct ttcgagggag aggagactat catttcagag aaatttgcct gtcctggatg
     8221 cgatatcact ctgcccgaga tagaaccgag gcttttttca ttcaacagtc ctttcggggc
     8281 ctgcccggat tgcaatggac tgggcagcca ccagtatttt tcggaagaac tggcagtcga
     8341 accgtctgtc tctccctttg aaggtgcact tctgccctgg aagaacaaac actacatgct
     8401 tcgtaaactc aaatctcttg ctttacacac gggctggaaa atggacagcc cttttcgtga
     8461 cctcgaaccg gaggtaagag aggcaatact ttacggctat tcggtgaaaa tcccccttac
     8521 cttccgcgag ggcagaaaag agatgtcata tatgggcagg tacgaggggt tgatcccgtg
     8581 gcttgagaca cggtggaaag ataccgaatc ggaatcggta aggaacgaga tagccagctt
     8641 tagagccgaa gatgtttgcc agtcatgcgg ggggcggcgc ctccgtcctg aggcgctttc
     8701 tgtcaaggtg gacgggctga cgataggaga agttgttgac atgcccgttt cagagctcct
     8761 tgagcgcctt atgggtgcca gggtgcatga attcatgata gagcccgcaa aaaggataat
     8821 tgaggaaata tcgaagaggc tttcttttat ggttgacgtc ggagttggat accttaccct
     8881 ttccagaagg gctgataccc tcagttctgg tgaaagtcag aggataaggc tcgcaaccca
     8941 gatagggtcc aaactgagcg gtgttcttta cgtccttgat gaacctacta taggccttca
     9001 cccgagagat acaaccaggc ttctgggaac gctggaagcc atccgggatc tggataatac
     9061 tgtacttgtc gttgaacatg accgggatac catgacaagg gccgactaca ttattgaact
     9121 ggggcctgcc gccggagaat ttggagggga ggtaacagcc aggggcaagc cgaaagagat
     9181 agccagaaca tcctcccttt ctggcccatt tctgactgga agggaatccg gtctctcgat
     9241 aaggaaaaaa catctcaaag cccccgaagg cttcataaac attagaggcg cgggagaaaa
     9301 taacctgaaa aatatcgacg taaagtttcc cctgggcaga cttgtctgca tcagcggcgt
     9361 ttcgggatcc ggcaagagca ctctcatgaa cgaggtcctc tacaagggtc tgagaagaaa
     9421 acttgacagg aattttcgtg agagagcggg aaagcatacg gatatagagg ggtgggaggc
     9481 cctgaaaaac gtcatcatga tagaccaggc tccaatcgga agaaccccgc gctcgaatcc
     9541 ggccacatat acgcagcttt ttactcccat aagagagttg tttgcatcgc tgcctgaatc
     9601 ccggatccgg ggttacggcc ccggacgctt cagctttaac gtcaagggag ggcggtgcga
     9661 ggcctgccgt ggggatggtg aggtcaggat atccatgctc tttatgcctg atgtttacgt
     9721 caagtgtgac gtatgcaatg gcaaaagata caacagggag actcttgatg tccgttacaa
     9781 ggggaaaaac atatctgaag tacttgatat gccagttgaa gaagcgggag tctttttcgg
     9841 tgatattccg aggatagcaa gaaggcttgc tgtccttaag gaagccgggc tgggctatat
     9901 gaggcttgga cagcccgcca cgaccctcag tggcggagag gcacagaggg tcaaacttgc
     9961 cgaagaactt ggcaaaaagt tcaagggcca cacaatctat cttcttgatg aaccgaccac
    10021 gggccttcat tacacggatg taagaaaact aattgtcctt ctgcagaaga tcgttgacca
    10081 tggcaacacg gtggttttga tcgaacataa cctggatgtt ctggcttcgg ctgatcacct
    10141 ggttgacctg ggccccgagg gcggcggcgg cggcgggaaa gtcatggata cgggaacgcc
    10201 cgcagagata tccaaaaagg ccagaggaca tacgggacgg ttccttgcgg aatatctgga
    10261 tcagttaagg gggagggaag acaatgacag agcggcagga tgagaaaaat acgatggata
    10321 acgatatctg cttcggaagg aattcggtca ggtctgccgt tgcctccacc ccggacctgt
    10381 gttccagggt aatgatttcg gaatctcttt ctggaaaaga aaaacaggag atcacttctc
    10441 tttgccggga ttccggtgtt gcctggcaaa tggtaaaacc ggccttgctt gataaccttg
    10501 tgccagccgg aagacaccag ggatttgccg tcaggttgtc tgctgtgcca cttttggagg
    10561 ttgaggacct ggagtcccga aagaagttgt ttccggggcc cgatcttctt cttctgctgg
    10621 atcatgtcca ggatccgcag aatcttggag ccgttgccag aagcgctgaa gtttttggcg
    10681 ccggcgcgat tattctcccc aggagaagat ccgccatgcc gacgtctacg gtaatgagga
    10741 ccagcgcggg agccatagcc agggttccag tggcgggagt ggtcaatgtt tccaggacgc
    10801 tggagacgtt aaaaaaatgg aactactgga tagtgggact tcaccatgaa tcccgccagg
    10861 tactcgggag cattcctctt ccggaaagga cggtccttgt tgtgggcggc gaagatagcg
    10921 gaatatcccg acttgcggcc aggtcctgcg atcatcttct ccgtattccc atggttggca
    10981 gtacgggatc cctcaacgca gcgactgccg cagccatagg gatgtacgag tggaggagag
    11041 cggttgacaa gggagttgtg cacgagtaaa attcaacacg cactatcctg ggtggttagt
    11101 tcagcgggag aacgcttccc tgacacggaa gaggtcgcag gttcgatccc tgcaccaccc
    11161 accattgtaa tcatggggat gtccgtcaag ggcatccctt tttgttttgg catgtcctta
    11221 tggaatataa ttattatata caaactctgg aggtatgaga tgatcagggg gatagggatc
    11281 gatatgtgca gtatccctcg cctagcaagg gctctcggga acccccgttt cctggaccgt
    11341 gtcttcacag tcgaggaaag ggattatgta gcgcgaagtg gaggtaatcc gagacattat
    11401 gcttcggctt ttgccgcaaa ggaagccttt gccaaagccg gtgggtgggg aattgccgtg
    11461 gtgggactcc gcaacgtttg gctctcaaga gataaagacg gaccttccct tatgcttgct
    11521 ccccatgcca tgtctctggt cgaatccata aaagccgcaa cagcccatgt tagcgttact
    11581 catgaaggag atttcgccgt tgcggttgta attctggagg ggtgacaatc attcgttatc
    11641 tatttgatcc cgtcgatatc cgtgaagccg acaggagggc catgtccgaa attggtgttc
    11701 cgggcacgat tctaatggag aacgctggca ggggagttgc cgatgtaatg accgcgcgtt
    11761 tcccgacggc aagaaagttt ctttttgttt gcggtccggg taacaacgga ggggatggac
    11821 tgaccgctgc caggcacatt ctggtcaggg gaaaggaagc agtggtgatc cttgcgtgtg
    11881 ccgagggaaa gttgtctgtc gaatcgttgc ggaaccttga tatcctcaaa aatattggct
    11941 gcccggtgcg atactcttct ttcctttcaa cggaagagat aactgcttcc gctttggaaa
    12001 acgatattgt ggtggaagca ctccttggga gtggttcctc gggggctcca agaggcgaga
    12061 tcctgagggt aattaaagcg atccccggat cggctacggt agtagcggtt gatgttcctg
    12121 cgggagttga cccttctgat gggagggtag aaggattcgc gctaagggct gacctgacgg
    12181 ttacgatgct cgcggaaaaa accggactgg agataatgcc cggaagggaa cactgtggag
    12241 atatcgaaat tgttgatata ggcattcctc cggcttcagt cctgcccgaa aagagcggag
    12301 tttcgatctt cctcccgtcc gacgccggaa ggctgcttcc tgaatggagt gggacaaccc
    12361 acaaggggag cagaggattt gttcttgttg ttggaggaag ttccaggtat tggggggctc
    12421 ccgtcctgtc tgcccttggt gcgctgaagg caggcgccgg ttgcgttgtc gtggcggctc
    12481 cagaagaaag cgccgcattc gccgggggct atcctgagat gatctttacc gagacaatct
    12541 gcgatggagg atttctctca ccggccacat gggaccttgt gatcgaacaa tggggtggaa
    12601 ggatcgattc catagtggcc ggaccaggca tggaccgggg aaaacctgcc gaggaacttt
    12661 ttacaaggat ctggaaagaa tgggagggat ctctctgcgt ggacggagat gccctccact
    12721 ttcttggaaa gggccaaggc ccgttcccac tgaggggaaa taccgttata actccccacg
    12781 aaggagaggc agcatccctt ctttcgaggg aaagacccag tgtttccgcc tccaggctgg
    12841 aagccgtcag gacccttttt aaaaatttcg ggacctgtgt ccttaaagga gcatcttccc
    12901 tggtttgcgg tgattcaggg gtccgggtta tcccctataa cctgccgggg ttgtctgtcc
    12961 cgggttccgg ggatgtcctt tcgggtgtgg caggagccat gctgggtatg gggatcgcag
    13021 cagaggacgc ggccgcctcc tctgcttgcc tgcatgccat agcggggtct ttcctccagg
    13081 aagagagagg ctttaacggc atccttgcaa aagagatagc catggccctg cccatggcca
    13141 ttaagtttgc ccgctccggg gcagggctta acagccattg aaaatgaaag gtcagataat
    13201 tgttaccggc tctgccgagg agacgagaat ggttggcgaa caaatgtcag gggtggttgt
    13261 tcccggagaa tatgttcttt tgtatggtcc ccttggtgcg ggcaaaactg agttcgtaag
    13321 gggttttgcc aaggggtgtg gatgggaaga agtcaggagt ccgtccttta ccatagttaa
    13381 cgagtactat tcggaacccc cgattatcca tgtggatttt tacagggtcg acagggcttc
    13441 atcggaagag tttgcccttg aggaatatgc ggcggaaggt tgcgtcgtga tagtggagtg
    13501 ggcggacctg tgggaatctc ccgatgactt gccggcatgg gaggtcaggt tcagcccctg
    13561 tgaagaagag caaccgggtc aggacgatcg gaaaagatac atcgagattt ttcggcgcgg
    13621 aaaagaattg ccttctatcc ttcaaagcag gttccaggta tctgaccgaa gaggaaagag
    13681 gatggagaaa tgattgtcct tggaatagac tgttcgacaa aaggtaccaa cgtgggagtt
    13741 gcgaggaacg gaacaataat ttcagaatat aatgtgtcaa taggaaggcg gcagtcggcc
    13801 gatcttccgt ggcttgtcga aaaagccctt tccgatgcag gaattgacct gcgtgaactt
    13861 gacctgatcg cctcctcttc cgggccgggg tatttcaccg gcataagggt ggggatttcc
    13921 tatgccgccg ccctggctga aggtctggag aaaaaaatag tgttgcccga ttccctgcac
    13981 tgccttgcgg cgcctttcct tgacggaaac aggagggtta tgtctgttat ccgttcaaga
    14041 aagggattct tttactattc cgttcttgaa ggccaatact cagagcgtcg cctgataacg
    14101 cccccttcac ttgagtcaac cgcagaactg atgaatatct gcggaatttt caatccttcg
    14161 gttctggtcc ttgacggcga tccggttttc cgggaggaga tgaagattcc cgattctgtt
    14221 tatgccgagt gggtatctgt aagaggggga tgggtcgccc ttatcggaga atctctctca
    14281 aaaaacgcga tacctccatc agaagcaagg ggttactaca tcagagagcc tgatattgga
    14341 caaagaaaat tccatgaatg aaaaaaagtc actgttcaag ttattcaatg aagtgtatta
    14401 tatttttcgt cgaccttaca agtgaaaaag tgaacatgcc tgatcccggg gaagaaggtg
    14461 gaatatggta acaggttcga aacaggaaat atttttacga atcaaaggag ggatgcggga
    14521 ttatggatct gatcgagaaa cacccgatac tggatttcaa ccacggggaa aaagtcaagt
    14581 tcacctttga tggtgcggag atggaaggtt ttgaagggga cccgatagct gccgcgctcc
    14641 acgccaatgg agtcagggtt taccgcgaga ctcccatcaa gaaacagacc aggggttttt
    14701 tctgcgcaat aggaaagtgt tcttcgtgct ttatggttgt cgatggagtg ccgaatgtcc
    14761 gcacgtgcgt tacccctctg aagagtggca tgcgggtcga gacgcagcga ggcaagggcg
    14821 tcatcgctat tgacgccgag tagagaggaa ggtggattcg ttgcgtcaga ctgaagttct
    14881 tgtcataggc ggaggtcccg ccgggttgag cgccgctctc gaggcggcat cccttggttg
    14941 cgaggtcacc ctcgttgaca gtgacctggc tctgggcgga cagctcatca aacagaccca
    15001 taaatttttc ggatcgaaag aggaacgagc cgggacaaga ggttttgtca tatcccggat
    15061 gcttcttgag gaactggata ctttcaagga aaaggtaaag gtcttcagca attgcactgc
    15121 cacgggattc tacaaggaag acggagtggt ttcctgtgtt gtgggtgaag aggaatattt
    15181 caggatcaag cccaaaaaga tcatcatggc aaccggggca caggaacgtc tgctgccctt
    15241 cccgttgaat catcttcccg gagtttacgg tgccggtgct gtgcagaccc ttatgaatgt
    15301 ttatggcgtt gtaccaggaa aaaaagtcat catggtagga gcaggcaata tcggtcttat
    15361 tgttagttac cagctgatgc aggccggagt cgagatcgcc gggatcgtag aggcgatgga
    15421 taaggtcggt ggctactggg tacatgccgc aaaggtcaga aggctcggtg tcccgatcta
    15481 tctgcgtcat acaatcaggg aagcccttgg agaccaggtg gtttccggtg ccataattca
    15541 ggaaatagac gaaaaattcc agcccacggg caaggagatc aaggtagatg ccgacgtgat
    15601 ctgtatggcc gtaggcctta ccccgacctg cgaacttctg tggcaggcag ggtgtgaaat
    15661 ggcgttcgtc ccacagcttt gcggccatgt ggcccagcgc gacctcaaca tgcggaccag
    15721 ccatcccgac atctgggttg ccggtgacgc ggcgggaatc gaggaggcct ctgccgccat
    15781 ggtcgaaggc cggatggcag gtgtcggagt cgccaagtcc ctcgggcacg gtgacgcccg
    15841 ggatctgtcc aagaggatgg aagaatatcg gcgtcgcata ggggatctca ggggaggcga
    15901 ggtcgggcag aagatctgcc agggcctcca gtgttgcact gtcgacgagt ggggggtgtg
    15961 ctccattgac tgacaaggtt aagctcttca atggcggagt tctcaccgaa cctcgcaagg
    16021 gagcaataga accgccgaag gaactctggg aggggaaaaa gaacggcctt gtaataatcg
    16081 agtgcccgca gagaattccc tgcaatcctt gccatacaag ctgccccacc ggggctgtca
    16141 agcccttcaa ggatatcaat gaccagcccg agatcgatta tgacaaatgt accgggtgcg
    16201 ccaggtgtgt agccgtttgt cctgggctgg cctgtatcgt tgtggatcta acctggggag
    16261 gaagcgatgt cgctcttatg aaactgccct atgagatgct tccccttccc gaagagggac
    16321 aggaagtcga atgcctgaac cgcgtcggcg aagaagtagc caagggcaag gtcgtcaagg
    16381 taaccgaacc gatgaacgac aggaccaaag tcgttcatgt tgagattcct ggatccctcg
    16441 taatggaaat caggacgatc agggtggtga agtagccgtg agtgacaaaa tgaccaatga
    16501 tatagttatc tgccgttgcg aggaggtaac ccttggagag atcagggagt ggatagccaa
    16561 gggctacgag acatttgacg aactcaaaag ggtacttcgt gtcggaatgg gcccctgcca
    16621 gggtagaggc tgtcgcgaaa tgatcctgag agagatcgcc cagaaaacag gcaagaccgt
    16681 tgccgatatc gatccaggga cattccgtca accctcaaag ccggtcaagc ttgcagtttt
    16741 ggccgagggg ggagacaagt aatgacctgg aagagaaccg ccgatgtcgt tatagtcggt
    16801 ggcggcgtgc agggttgctc tatcgcctat aatctggcaa aactgggcgt cagaaatatt
    16861 gttgttctcg aaaaagggac tatctgttcc ggttcaactg gcaggtgcgg cgcggggata
    16921 cgagcccagt gggggaccga gatgaactgc cgtttcggtc tcgcttctgt tgagaagttc
    16981 gaacagctcc acgaagagct tgggatggac tgcggtctca accagagcgg gtatctcatg
    17041 gttgcctaca aggagagcga gttcgagcaa ttgcagaaaa gcatgaaact gcagaacagc
    17101 cttggcatta acaccagggt cgtaagttac gatgaagcca gggagatatg cccgggactt
    17161 gccgcggagg atgccgtcgg gtttacattc cataacaggg atggtcacgc cgatcctttc
    17221 ctgaccacat ttgctttcat ggaggccgcc aaaagactgg gggtcgtttt ccacagattc
    17281 acggaagtca ctgatatcag gaccgttgcc ggaaaggtcg ccggtgtctc cactaccgct
    17341 ggtgatatag atgctccggt agttgtgaac gctgcgggtg gatactctca attcatcgca
    17401 acgatggcgg gggtggaaat acccaatttc tccgagaggc acgagattat tattaccgaa
    17461 cctgtcgagg ctggtgtgtg tcccccgatg cttatgagtt tctcgggcaa ctactacatc
    17521 cagcagaggc cccatggctc tataatcggg ggatgcagcc ccgaaggaca tccccaggac
    17581 tatgaacttg gagcctcctg ggattttatc gagatgatgt ctcgtacctt tgttaaactc
    17641 cttccccgca caaaaggggt cagggttgtc aggcagtggt cgggactcta caacatgact
    17701 cccgacaagc agcctattct ttgcgaagcg gataatgtca aaggttttta cctttccgtt
    17761 ggttattctg gacacgggtt catgttcgcg ccgatatccg gggaacttct tgcccagaag
    17821 atcactggaa aaacaccatg gatcagtatc gacatgctcc actacgacag gttcgaaaag
    17881 ggagaacttc ttgtagaacc agcggtcgtt tgattttccc agaggacagg gagaaatagg
    17941 ggagggatag ccgaaaaggc ccctcccatt tttttgtccc aaaaatggtt aattatctca
    18001 attgaacgag aattatcctt tgtaatgcaa tattcaatat atagatacaa atatcacttg
    18061 tcccccgact cacaaggtgg cataatgact tgcagataaa tattttccgg aaaaagaaac
    18121 gatcgttttg aaaggaaggt gagttgtttg ccgaaaagat tcaacgttga agtgaactcc
    18181 gcttggtgta agggttgtgg cctgtgcatg gccgtatgcc ccaagaaggt tttcgatttc
    18241 gatggaaacc tcaaatcaac tccatccagg gtccaggatt gcataggctg caagcagtgc
    18301 gagaatatct gtccagacct tgctattaca gtggaaggga gtggagaaga tgcccagaaa
    18361 tgaattctgg cagggtaacg tctcccttgc ccagggagct ctggccgcgg ggtgcaggtt
    18421 ctttgggggc tacccgataa ctccttcttc ggaaattgcc gagactatgg cagaagagct
    18481 tccggcactg aacggcaggt ttatccagat ggaagacgag atagcgggta tagcagctac
    18541 cataggcggt tctgtagccg gatataaggc catgactgcc acgtcaggcc ccggtttttc
    18601 attgaagcag gaaaacctgg gtctcgccta tatggctgag atccccattg ttgtcgtaga
    18661 cgtaatgagg ggaggtccct cgacaggcct tccgacctct attgcccagc aggatgtgat
    18721 gcaggccaga tggggatccc acggagacca tgccacgata gcctatgccc cttcttctgt
    18781 acaggagtgt tacgagatag ccatcaaggc tttcaatatg gctgaacgtt tcagacagcc
    18841 cgttctgata atgagcgacg aggttctggg acatatgaga gaaaaggttg ttgtccctga
    18901 agagggttca ttccccctgg tcaatcgtaa acgtccttcc tgcgatcctg aaaatttcat
    18961 tccctacagt ccagatccgg aagacaacct gccccccatg gcatcctttg gggacgggta
    19021 tcgctggcat attacgggcc tgacccacaa cgagtgggga tttccgacca actgtgcttc
    19081 ggagatcgcc aaaaaatcgg caagactgat ggacaaggtc agtgcctaca gggacgacat
    19141 cgtcgaatat gaaacagaaa gtgtcgatga cgcggagatc atcgtccttg cctacggaag
    19201 cgttgcaagg actgcactga gcgccgtaag gcaggcaagg aagaacggaa tgaaagtggg
    19261 ctatttcaga cccataacgc tgtggccttt cccagatagg gagatcgagg ccctcgcggg
    19321 aaaatgcgcc tccatcctgg tcccggaact caactttgga caaatgatac tcgaggtcga
    19381 gcgtgcggtg cacggaaaag cggatgtagt ccagatgagc cttgtaaacg gtgagctgtt
    19441 ccagccctcc gagatcctgg aaaaaatcga ggaggtggcc cgataatgcc ccgtcctgaa
    19501 gtttttgact ggctaaggaa aaagttcttt ccccacatgt ggtgtccggg ctgtggtcat
    19561 ggcatcatca tgcacgcgat cctgcgcgcc ctgatcgatt cggggaaaaa gagagaagag
    19621 acagtaatag catccgggat aggttgttcc agccgtatgc ccggctacct tgattgttgc
    19681 acgatgcata ctacccacgg gcgttccctg gctttcgcga caggcatcaa gcttgccaat
    19741 cctgaactga caataatcga tgtcatgggc gacggtgact gtaccgccat aggcgggaac
    19801 catttcatac atgcatgccg gcgcaatatc gggattaccg cagtggtaat gaacaacaat
    19861 atctatggaa tgaccggggg gcaggcttcg ccgaccacac ctctcggggc caaggctacg
    19921 acagctccat atggtgccat agacccgccc ttcgatgtct gcaaactggc ccagggagcc
    19981 ggggccacct atgtcgccag agctaccata gcccagccgt tactttgcga gcagtatctg
    20041 acacacggta tcgccaacaa gggtttctcc gttattgaga tcatttccag ctgtcatacc
    20101 cagtttggaa gaaagaacaa caggagaact cccgttgaca acctgaatta cctcaagaac
    20161 gcatcggtcc ctctggccag gtccgaaacc atgacgcccg aagaactcga aggaaagatc
    20221 gttacaggtg aatttgtgaa aagagaatct cccgaatata ccgatcttta ccttgacctg
    20281 atctcccgcg taaggggggg taagtaatga gtgatcgtta cgagatccgt tttgccggtt
    20341 ccggtggcca gggtgttatc cttgcggcgg ttgtggtcgg tgaagcggcc gcaatttttg
    20401 agggcctgaa cgcggttcaa tcccaggcct acggaccaga ggcacggggc gggaagtcca
    20461 aatcagaggt cgttatttcc agggacggga tcgactatcc caaggccact gccccgaatc
    20521 tgcagataat cctgaaccag acttcctgcg acgagtttgt ggctgacacg gcaaaaggcg
    20581 gagtggttat cgcggacgac acttacgtga attccgttcc caattctgat gccgaggttt
    20641 acatgcttcc tattgtcaag accgcaagag aaaagattgg cagggaactt gtgaccaata
    20701 tggttgcact gggtgtaacc gcaagggtcc ttgaggctga aggcatgatg aaacctgaat
    20761 ctctcaagaa ggccattctt caacgggtac ctaagggcac agaagagatg aacgaacgtg
    20821 ccttcaatga agggtacgaa ttgatgaagg caaaaaaata aacagcggca taactgatat
    20881 aatttcaggg gcgggggggt tatgaccccc cgcccctttt gatttatgat gccatggcag
    20941 ggttacgaaa aggggtgggt ggtgtttttg aaggatagtg aagggaagat catcagcgcc
    21001 aacgttgaga cagtcaacag ccggggggac ggtgtcgtca ggtcaggcga tgaccgtttc
    21061 gttttttttg tgcccggtgc cctgccagga gaaaggatca cgggtaaggt cgtcatcgta
    21121 aagaaaagct acggagtgct ggatctttta gaggtgaagg aacccagccc cgaacggata
    21181 gagccccgat gtccgtggta cggaagatgc ggagggtgtg cccttcagca tgcttcatat
    21241 ggcttccagc tcaagatgaa ggataccgtt gtcatggatg cgttcagaaa ggtggcatca
    21301 atggttttgc ctggctttga aggatgtgtg ccgtcaccgc tccaatgggg ttatcgcaac
    21361 aaggcctctt ttcccgtgca gatgtccaaa ggtgtagcaa gagctggttt ctatagccag
    21421 aaaagtcacg atcttgtccc tgtggatgtc tgtcctgtcc tggaagagag gctggaaagc
    21481 tggataggtc ctatcgggtc tgccctgaaa aggagcggga tccaggcata cgatgaatcg
    21541 gaacataatg ggctgcttcg tcatattgta gctcgctgtg ccggaagaac ctcgtcttcg
    21601 atggtggttc ctgtggtatg ccttgcccct ggaaaagaca ttcctgccga attaaaaaac
    21661 cttgcagaca tgttcatgga ggaattctcg ggtgtcggcg gtgtcaacat taatttcaat
    21721 cccttgagag ggaacacaat ccacgggaga tactccaggc ttgttgctgg tgatggttcc
    21781 ataactgaca atatttgcgg tttttccgtg aaatacggcc ccacgtcttt tttccagatc
    21841 aattcacttc aggcggagtt ccttttcaag gccgcggttg aagaactgga tctgctggga
    21901 tgcgaaagag tcctggaact ctacagcggc actggtgcac ttacgcttaa ccttgccaga
    21961 ggcaggaaga aggtccatgc cgtggaggaa tggccagaat cagcaggtta cctcagggag
    22021 aacgccttga tcaacggggt cgaaaatatt catgttcttg agatgagttc cgagaggggc
    22081 gttcaatttc tggggagggc gaaattcgat gtcgttgtag tggatccacc aagaaagggt
    22141 tgctccgagc atgttctgaa aggaataagc gcgatagacc ccgaatacgt tatttatatt
    22201 tcctgcaacc cgtcgaccct tgcccgggat tgtgcatttc tcatggaaaa gggatacaga
    22261 cttcgaaata tcagaacttt tgatatgttc ccccagacat tccatgttga aaccatagct
    22321 acgatggtaa aagagaggag gaaaaaaaga aaaccaggtc ctgtggccgg aaaggtccga
    22381 gaatcgtgaa ttgtgaatcg ggtgactagt gtgaagcagg aaaaacaagg gcaaggcctt
    22441 ttgggttctc ggaaaaagat tattctgcgt tctgtggcgc aagaacctga gggattaaag
    22501 ggtaagctgg aggggcggaa aaacgagtcg gcaggttgaa tatgcctggt ggccttgagg
    22561 tgcagaaccc tgatgcggga ttggatgctt gtcaaagcgg ttgacccaat ctataatgtt
    22621 ccatcgaggg cggacatagc tcagtggtag agcatcggct tcccaagccg agggtcgcgg
    22681 gttcaaatcc cgttgtccgc tccattttta tcctcacaga cagtttgccc ccgggtatgt
    22741 gttcatgggc aattcgtgat tattctgatg aaaccgtgta tttcccgttt gtagaacaag
    22801 aaaaatatga ggcaatcttt aggttgacat gagaagggac gcccattaaa atcatgaagg
    22861 tttgttaaaa atttctcttt atacagtatt atgcatgcta ggggggtaat ttgcgtggcg
    22921 aagaacatcg cggatgaaat taccgaagcc gaggcaaagg gaaaacagat ggtccaggac
    22981 gcccgtaccg aaggggcccg gatatttgcc caggcgaaga gtgaatcgga ggatatggta
    23041 aaggcggcga agcagaattt tcacaggtca ttccgtgagg ctgtagtcaa acttgaggct
    23101 gaggcagaag tcgaagcgga aaagatcgta gaaaagggga gagctgaggc cgagaagtac
    23161 gccagggcac atgagagcaa cattgatgat gctgcagcat gggtcgccga agaggtgatc
    23221 ggtcgatatg gcagtaacaa agtttaaaaa aatcaggatc ctttcgcatc acgaagcact
    23281 ggatcatgtc atcgcagaga tccagaagat tggatgctgc gagataattg accgggatga
    23341 tggtgggaac gaaactggat ccgtagtttc aacttcagac aggtcccggg acaggggatc
    23401 tctggacctt gattcgagtc tttcagacgt aaggtttatt ttgcgttttc tcgagcctct
    23461 gtacaaggaa gagggcggga agctcgaaag aatccttgga cccaaacctt ctgtttcatt
    23521 ctctgatctt tccctgctga aaaagagtac cgatatttcg atgatcaatt cagaagcccg
    23581 cgcaattgag agacaactta cagagacccg ttcagaactt gcccagatag ccaaccagtc
    23641 acttcttctt gattcaataa aaggatttac tcttcccctc tggatgatat caagggggac
    23701 cgggaaggtc atgggagtga tgggaaccat cgcttcctcc caggcatcgg cccttgagtc
    23761 tgatttcagt aatgcctcac aaggggaagg gcatatcttt atcgctccgg tgttagaaaa
    23821 acagccggag acaaatgccg tgctggtttt ctcccgttcc cgtgagagtg ccgtaatgga
    23881 attggcagtg aagaacggtt tttccaggat tgagtgtccc cagggtctcg aaatgactcc
    23941 cggagaggaa ctggaaaggc tggaaggact tgcggccgaa ctcaaaacaa gacttgaaga
    24001 ggcagagaaa aaagcatata gcctggccca gagctgggtt ccgatcgtca gaaagctcaa
    24061 cgattttctc aatgtttcca tagcccgtcg tgatgcagcc ggatcaggcg aatctacaac
    24121 gatggtttcg atactccgtt tttgggttcc cgcggacaga acaggtgacc tcgaaaaggc
    24181 attggcgcca tattccggtt tgatcgatgt gctcaccgaa gatcccgcac cggaggatca
    24241 ccctccgata ctactggaca acccgctgtg ggcacggcca tttggtcccc ttacaaaact
    24301 ttatggcatg ccatcgtaca agggtattga tccaaccgtc ctgatggcgc cctttttctt
    24361 cctcttcttt ggtatgtgcc tcggcgatgg agggtatgga ctgataatgg gagcgttttt
    24421 cctttttgcc cttgtcaggt acaagctttc cggcgacacc aaaaactttt tcgttctgct
    24481 gttgctgggt agcgtttcta cgataatggt gggggccgtt accgggagtt ggcttgggga
    24541 catggtcgat gccttcggaa tattctcctt catacgtccc ctgaaagaaa gcgtggttct
    24601 cctgaggccc atgagtgacc cgatcacctt cctgggtatc tctctggccc ttggagtagt
    24661 ccagatcctt ttcgggatac tcatagcaat gtgggatgcc ctacgtaagg gagacattat
    24721 agcggctctt ggagaccagg gcggatggtt catgctcata gtggggatac tggtctgggg
    24781 agctggatcc atgggaatgg gcggagaggg cttgattctg acaggtaaag gtcttgccgc
    24841 attgggtgca cttacgctgg tggcgaccca gggacgtgaa aagcccggag tcattagaaa
    24901 agccgttttc ggggttctca gcctctacaa cgtgacttcc tatcttggag acgttttgag
    24961 ttacagcagg ctcctggctc tgggactcgc gacatcggcc attgcaatga tcgtgaatac
    25021 ccttacaaac ctggtctccg ggattcccta tgtcggctgg gtcctggcgg cgttgatctt
    25081 tttcgggggc catcttttca gcgttgtcgt caatgttctt ggagcgttca ttcattcatt
    25141 gcgtcttcag tacgttgaat ttttcagcaa gttctacagc ggtggaggcc ggatattctc
    25201 tccgttgaaa ttcgagacca ggtacacgaa cattactgac ggtaccatag agtaaaatta
    25261 ccggcttgat gccggaacga tacaatcatt ttccagatcg tctgagagga gtgtttctta
    25321 ataatggatc agctcgggat tatgctttcg gttcttggag cggctcttgc cgccggtttc
    25381 gcaggttcgg gttcggctat aggggtaggt atcgcaggag aagcaggcgc gggtatcatg
    25441 actgaggatc ctggcaagtt cggtcttgtt cttctgctcc aggctctgcc tggaacgcag
    25501 ggtatatacg gtcttcttac ggcatttttc gccattctga aggttggtct actcggtgga
    25561 tctgctgtac ctgtaaacgt ttggcagggt ttgggggttc ttttcgcttg tcttccgata
    25621 gccatagcgg gttatttttc tggaatagcc cagggaaaaa catctgccgc ttgtatccag
    25681 atgattgcca agaggcctga agagaccggc aaagctgtca ttcttcctgc gatggttgaa
    25741 acttacgccg ttcttgcgtt gctgatgagc atcattcttc ttaacagcat ccagttctag
    25801 gccatcgtca aagacggggt gagagtaatg tctctagagg atattaaggc caggatcgag
    25861 gcggatgcca aagccgaggc cgaaggaatt acaggaaaat tcagggatca ggctgatgag
    25921 gttatgaagg ttgccaaggc cgaggtggat aaacttgaaa aggccactca atccaaaatc
    25981 aaaaccgaag agaccgagat tctcaggaga agaaagattg tagctgaact ggatgttcga
    26041 aagatccagc ttggtaccaa gagggacctg atcgacaagg catttcaaaa ggctctttcg
    26101 atcctttcaa aaatgcccgg cgaaaaatat gttcctttta tgacgggcct tcttgatcgt
    26161 tccgtctcct ccggagacga gaccgtttat gtgggaaagg aagagaagat ccttgatgaa
    26221 aaatggatct cggatttcaa cagcagaaaa aagacgaaac tgaccctctc cagcgaatgg
    26281 gtccctgctt caggtggttt tgttgccaga agaggggata tttatgtcaa ctgcacctgg
    26341 gatgttcttc ttcgcaggat cagggaagat ctggagacag aggttgtcaa ccgtctattt
    26401 ccgaactgac cggggggctg aagcatgacc cgttcaatag attacggcta ttccgtatcc
    26461 aggctcaggg cgatggagaa ccgtcttctt gaccccggca gcttccagag gcttttagac
    26521 agtgaagatc tttcctcagc catgagggtt ctttcggaga ctgtctacgg gaaatggatg
    26581 cttgaacagc agcgtgagga acagttcgac caggccattg agtcggaact tcagtatgca
    26641 tattccgaga ccgaaaaatt tgttccggat cccgggcttt accagctttg caggcttcag
    26701 tacgatatcc acaatattaa ggttgtcctt aagggtatct ttgttcataa acagggcggg
    26761 aaaagaagga tggatcttct gaccgggctt ggaaatatca atgtggatga tcttgttgtg
    26821 gccctggaaa gcgaagacta ccggcttatg cctttcggtt tccaccggac cgttcccgag
    26881 tgtgtaatgt tatgggagca gacccacgat attttgcagg tcgagactgt ccttgacagg
    26941 aaacttttta ccctgcagtc cgatcttgct tcttcgatgc ccttcgaagg agcaagactt
    27001 ttcgtgaagc acagaattga ctcagaaaac ataaggaacc tggctcgcct gaaaaggatg
    27061 ggttacgagg ccgcccaggc atctccgttt tttcaccccg gtggttttgt ctctgctgaa
    27121 aaaatagtct caatgatcgg cgaacccctc gaaggctggg aaagaattct ttcttttgct
    27181 gatgtttcca gggccctttc aggagcacag gaacattctg gcattgattc gctactggta
    27241 gatcttgaaa aatcaatcga tgatttcctt tcttccgtcc ttgaacaatt taaatatagt
    27301 cccttcgccc cggagaacgt tcttaatttt ctctggaaaa aggagatcga ggcgaaaaat
    27361 gttcgtattc tccttgtcgg gataggcaat ggcgctgaaa gaagtgttct taggaggctt
    27421 ctcagacatg tctagcgaag ttaagaagct caagatggca gccgttggag attttgagag
    27481 tgtcctgccc ttccaggctg taggtgttac tcccttcatt cccgaagggg gcctcacgga
    27541 actccttatg aggctttccc gtgagaaata tgccgttgtt ttccttgtcg acgaccttta
    27601 cgcccaaaac agggagagca tcgaggagat caacgaaact caaaaaatca gcataatccc
    27661 tgttcccggg ataaaaggaa ccacggggat cggtattaag actatccgtg acagtgttga
    27721 gagagctgtc ggtatggata tctttgctgt caaatgaact tgtctgaaga tgtattagta
    27781 tgcggagggg atagtattgg tcatgaagga taaaattgtc ggatccattg cgaagatctc
    27841 cggaccgctt gttgtggctg atggaatgag cggatccggg atgtatgacg tcgtaagggt
    27901 aggagacctg gggctcgtgg gagaaatcat cgaactcaag ggatctaccg cttcaatcca
    27961 ggtttacgag gaaacctcgg gcctcaagcc cggagaaccc gtagagagta cgggtgcacc
    28021 acttagcgtg gaacttggcc caggactcat cgagcagttt tatgatggtg tgcaaagacc
    28081 acttgctctt atcgagaagg ctgcggagag tccctatatc tcaaggggta tcaacgtttc
    28141 agccatcgac cgttcccgga aatgggaatt tattccctca gtggaaccag ggacagagct
    28201 gtgcgaaggg gactttatcg ggtatgtgca ggagacggtt cttgtaaagc acatgattac
    28261 cgttcctcac ggagtcaagg gcaaggtcaa aaaaattgaa agcggcagtt tcactgttga
    28321 ggaagtaatc gcggttattg attcgggcga taaagacgtc gatattacca tgctgaaaaa
    28381 atggccagtc agaacccccc ggcctgtttc ccgaagactt gcgccgaaca ttccgcttac
    28441 cacagggcaa agggttgtgg acaccttctt ccccctgtca agaggtggag cagcatgttg
    28501 cccgggtcct ttcggatcgg gcaagacggt tatacagcac cagctggcaa agtgggccga
    28561 agccgagata gttgtttatg ttggttgtgg cgagcgtggc aacgagatga ccgacgttct
    28621 acttgaattc ccggaactgg aggatcctcg ttcaggacac cctctaatga agaggaccgt
    28681 tcttattgcc aatacatcca acatgcctgt tgcggcaagg gaagcgagca tctatacggg
    28741 aatcacgata gcagagtatt acagggacat gggttattca gtcgctctga tggctgattc
    28801 gaccagccgg tgggctgagg ctctccgtga gatatcggga agacttgaag aaatgcccgg
    28861 agaagaaggg tatcctgcat accttgggac caggcttgca agtttctatg aaagggcagg
    28921 gcgagcgata tgtctcggat ccgattcaag agaaggctcg ataaccgcca taggagccgt
    28981 gtcgcctcct ggaggagacc tttcagaacc ggtcagtcag aacactcttc gggttaccaa
    29041 ggtcttctgg ggacttgacg cgaaccttgc ctaccagagg cattttcctg caattaactg
    29101 gctgcttagt tattcccttt attcggagaa gctcggagaa tactgggaca acaagtatga
    29161 tggtgaatgg agcggatttc gcaccgaagc tatgaccctg ctggaagagg aagacaggct
    29221 cagggagatc gtgaggcttg tcgggattga cgctctttcc agacaggaaa ggatgatcct
    29281 ggagacagcc aagtctttaa gggaagattt tcttcaccag aatgccttcc acgagatcga
    29341 cacctttgcc tcaatggaca agcaatacag gatgctcaga aatattatcg gtttccacca
    29401 tctttgcatg gatgctatag aacatggttc aacgatacag gatctctttt cccttcccgt
    29461 aagggaaggg attgcgaaga tgagatatgt tgacgaaaaa gaacttgata agatcgatga
    29521 acttcttccc gaaatgaaga aacaggtgga agaactgtcg ccggaagggg gtatggggga
    29581 tgttgcctaa ggaatacagg accatatcaa atctttccgg tcctctgctt gttgttgaga
    29641 acacagagaa tgtccggtac gacgaacttg tcgagatcga actcgttaat ggagagacca
    29701 gaaggggcag agtccttgag atcacccgtg atcacgctct ggtccaggtt tttgaaggca
    29761 cctccggaat agatctcgat agcacaaagg tcaggtttat gggtaaggtt cttactcttc
    29821 cagtgagcaa ggatattctg gggcgtatat tcaatgggcg cggtgaacca atagatgacg
    29881 gggctccggt catcccggaa aaatcgctcg atgttaacgg ttacccgatg aacccatact
    29941 ctcgagatta tccctcggag tttatccaga caggtgtgtc gacaatcgat ggcatgaatc
    30001 ctatggtacg tggacagaaa cttcctatct tttccggaag cggaatgccc cacaacagga
    30061 tagcggccca ggttgcgaga caggccacag ttcttggagg gcacgaggat tttgccgttg
    30121 tttttgcggc catgggaatt acctttgagg aagcctcgtt ctttatggag gatttccgca
    30181 aaaccggggc tattgaaagg acggtcatgt tcgtcaatct tgccgatgac ccggctatcg
    30241 aaaggatcac gacgccaagg cttgctctta catgtgccga ataccttgct ttcgaaagag
    30301 attaccaggt tctggtaatt cttactgacc ttaccaacta ttgcgaagct ttgcgagaga
    30361 tctcgtcggc aagaaaagaa gttcccggaa gaaggggtta cccgggctac ctttacactg
    30421 accttgcaac catgtatgag agggccggaa gactgaaagg gaaaaagggt tctattaccc
    30481 agttcccgat actgacgatg cccgaagatg acaagacaca cccgatcccc gaccttactg
    30541 gatacattac cgagggtcag atcattctga gcaggaccct ccacaggaag ggtatctatc
    30601 ctccggttga cgtcatgccc tcactttcaa gactcaagga taagggtatc ggtgaaggaa
    30661 aaacaaggga agatcacgcc gaccttatga accagctatt tgctgcctac gccagaggaa
    30721 aagaagccaa ggaacttgca actatactgg gagaaggtgc cctttccgag gaggataagg
    30781 cctttgcgct cttctcggac cgctttgagg attcatatgt aagacaagga gaatacgaga
    30841 acagaaccgt tcaggagacc ctggaacttg gctgggagtt gcttacgctt attcctgtca
    30901 gggaactcaa gcgcgtaagg gatgcatggg tcgagaaata ccttaagccc aggctcaagg
    30961 aatccgggga acaacggtag acctggatgg gcaaggagag ctgaccatgg caaaaaaact
    31021 tagcgtaaac ccgaaccgga tggagctttc caggctcaag atcagacaaa aggtcgcaaa
    31081 gcgtggtcac aagcttctca aggacaaaca ggatgcactt atcaaggcat tcctcgaaaa
    31141 agccaaaaat ctcaaggaat tgagagaaaa ggtagagaag gaacttgagg agtgttacag
    31201 gagttttctc cttgcaaggg cccagacttt tccggctatg ctggagcagg cactcatgat
    31261 caccgggagc gggctctcga tctcaatgtc gaacaagaac attatgagtg ttatcgttcc
    31321 ggtctttgag gtccagcagg gagaggccac tctcaactat ggacttggga caaccacggg
    31381 aaaccttgat gtggctcttg agaaattttc caggcttctt cccgagatgg tctcaatggc
    31441 cgcggaggaa aaggcgttga ggcttatggc tgtcgagata gaacgtacca ggagacgtgt
    31501 caatgcgctg gagcatgtcc tgataccctc cttccaggag actatcagat acatcacaat
    31561 gaagctggat gaacaggaga gagccaatct gagcaggttg atgaggatca aggagatcgt
    31621 aaggtctcat tagaaacgtt ttgggaaaaa ttagtcaaag agcctcttcc tttcagggaa
    31681 gaggctcttt tttgctcttt gcatgataaa atacatatcc gcggaaagta ttccatgcgg
    31741 tcgctgtccc gaaagggata gaggaaagtc cgggctccac agggcagggc gctggagaaa
    31801 ttccagtggg cgtgagccca aggaaagtgc cacagaaaca taccgcccgg cactcattca
    31861 atggcctgga gcaaggtgca tgacggatgc ttcattgagt cccgagtttt cagcaaagat
    31921 ccaggctatt gaatgagtgc cgggtaaggg tgaaaaggtg gggtaagagc ccaccagttg
    31981 ccgggtgacc ggcatgctag gtaaaccccg cccggagcaa gaccgaatag gaggggatga
    32041 ggttgcccgc cgaccctcgg gtacggtcgc ttgagacttg tcgcaaggct tgtcccagat
    32101 aaatgaccgc ttcacacaga acccggctta aggaatgctt tccgcggaaa tgatcctatg
    32161 ggaaagaaaa ggggcgcatc gttgaacgat gcgccccttt tcttgtgcat tgtataaaag
    32221 atagtgctaa ggttttaaat ggaataagac taaagggtca atagtactat ataacccatt
    32281 ttaaaggtgt taaagtggag caaagtggca taaagtggta aaaaatggga ggagaacccc
    32341 atgttggtgg ggacttacga acaccggttc gatcctaaag gaaggatagt ccttccatca
    32401 aaatttcgag acgaacttgg cgagaatccg gttgctacag tcgggataga ccagtgtgta
    32461 tcgatatatc ccgctgacaa gtggcaggca ctccttgaaa ggttccagaa gatgtcgttc
    32521 tcaaagggga ggaccaggga ttttctcagg gtgcttctgg ctacggctca cgagatacag
    32581 ttcgatgctt cgggcagggt gcttgttcct gtcaacctga ggcagcacgc aagggtggaa
    32641 caggatgcga ctgttatcgg tgtcggtgat cacatagaga tatgggactc cggttcatgg
    32701 aacatttatc tggagggcgt gaaaaaggaa ttgccagata ttgcggaaga ggttgaagga
    32761 ttatgagcgg gcacattcct gtgatgcttc ccgaggtgtg caaggtcctt gggtcccttg
    32821 agaagatcgg ttttgttctt gacgcgactc tcggttttgg cggctattct gaagcgatcc
    32881 tgttcaagtg gcccaacgtt gcagttcttg gggtagatca ggatccccag gcgatcgaat
    32941 cgtcaaagag aagacttgct ccttttggtg aacgattcag ggctgttcac ggaaacttca
    33001 gagatatagg agaaatcctg gaaagatccg gaatgggtgt tcctgatgcc atactttttg
    33061 atctgggggt ttcgagtgtt caggtggata cccctgaaag ggggttctcc ttccaggagg
    33121 ccgggccgct ggatatgagg atggatacgg cggacgagag gatgggaatg gctgccgata
    33181 tagtaaacaa ctacggaatg aaggatctga taaggatttt ccgggattac ggcgaggagc
    33241 gttttgccgc ccagatagcg agagggatca tcaagtaccg ggaaaaggaa gggcccgtaa
    33301 ataacacaga tacccttgtt ggcatcataa gaaacactct tccggctccg gtacaaagaa
    33361 aaatgggtcg taacccggca agaaaaatat tccaggcctt gcgaatcgcg gtaaatgatg
    33421 aactgaaggc gctggaggag gcattggaac agtgcgggcg tttttcgaaa gacagggtca
    33481 ccttggtggt acttagttat cactctctgg aggacaggat cgtaaaaaga aaattcctgg
    33541 agtggaaggg tttgtccctt ggagtgcttc ctttcaggaa acctcttgtt cctggtgaag
    33601 aggaacttga ggagaaccgg agatccagaa gcgcaaaaat gagagttttc cattttggcc
    33661 taaaggatcc gggggggaag aagagatgaa cggacagaat cctgtacttg aatattccgg
    33721 aaacggacgg atacgaggca gaggctcgat ctgcattatg atatgcgtag taactgttgc
    33781 tcttctgatg gggctggtag ggctcaggtt atattgtttg aatcttgaat ataggttggc
    33841 agggatccag aaccagatag ttgcacgaga ggaggaacag tcgcgacttg agaaggatct
    33901 tgccagttta ctgaatccga caagagtgta tagttatgcg aggtcggaac tcggtatgag
    33961 ggcttctgca aatataggcc ttgtaaggat tgccggaaca gaaagcagac cctccgggcc
    34021 ggtttcgccg ggtcccgaga cgggaaagtt cagaagcgcc cttgtcaggc tggttgacgc
    34081 cataacgggc aaagcctctg cgcgacagta agatccacca tcactgaaag gaacgggcaa
    34141 taaataatga taagcctgag gaaatctccg tggtttttta tgtttctgtg cgttcttgcg
    34201 atcctcttca ggctctattt tctgcagatg gaaccagatc cgagagtgag gtcccaagcc
    34261 tccagccagt actgggccag gctaaaagtc agtgcccagc gtggatacat aagagacagc
    34321 aacggaatat cccttgcctt atcaaccccg gcattcagct tttttatcga tcctaccata
    34381 tgggacccca caaatgctcc gcttctcaag gatcatatct ccggcacaaa actcgaaaaa
    34441 atttccggga gaatggaagg aaggtattat caggttgccc gaaaagtcac gcccgatgat
    34501 gctgaaaaaa tgataaggat gaaacttccg ggacttttct gggtcaagga gacccgcagg
    34561 gtataccccc aggggcgcac cctttcccat gttcttgggt attgcgatat agacgacgag
    34621 ggacttgccg gcctggaact gttctggaac catgcccttt atgctccgcc tgaaagcagg
    34681 ataattgcca aggacgccaa gggaaatttt gtggatgttg caaggcccgg aaaacctgtt
    34741 gcgcccgaag gtcagggatc agtaggtctt acaattgatt ccaggcttca acatatatta
    34801 gaaagaagac tcagggaggg tgtttcaaaa ttcaaggccg agtgggccgc cgcggtgctc
    34861 ctggagccgg atacaggtcg tgtacttgcc atgtccagcc tgccggattt tgatccaaac
    34921 gacaggagca cattcagcaa tggagaatca ttgagaaata acgtgatctc aagggttttc
    34981 gagccgggat caaccttcaa accggtaatt ttgggtaatg tgatcgataa cggtcaggtc
    35041 tctaaaaata catccgttac atgcactggc aagatcaagg tcgctgatgt tgtgatccat
    35101 gatataaaag cccatggcaa gatcgacatg acccgggtta ttgcggattc ctgcaatgtc
    35161 ggcatggtaa tgagtgttga gaagtccgaa ccgcttcaga cctggtcctt cctgagaaga
    35221 tgcggtttcg gatccatatc gggggttgag attcctggtg aagaaagcgg tctgatgact
    35281 ccgccctccc agtggagagg ttcaataccg gctactatat ccatagggca cgggattgga
    35341 gtaactcccc tgcagcttgc tgttgctatt tccgctatcg ctaacggggg aaggataatg
    35401 aaacccttca tggtttctga ggtcctttcc agggaaggaa atgtcctcta cgcgggaaag
    35461 cccacggtta tggccgaggt tttttccagt gatactgcca aatggcttcg ttcagcaatg
    35521 agaaaggctg ttgaaaatgg tacaggaaaa aatgctgacc tccctggtgc ccatatcgcg
    35581 gggaaaaccg gaacggccca ggtgcctggt tccggaggat atatcaaggg gtcatacacc
    35641 ccttccttcg caggtttctg gccaagcgaa gatcctggtt ttttacttct tgttgtgata
    35701 ggtaatcccg gctcgggtga ttttctcggg ggaaaggtgg cggctcccgt tttcagaaat
    35761 attgttcagg acattgagca gatctcaagg taatacctct cttccaagaa atgggggaaa
    35821 aggatgaatt taagggaact gacagatatg ttggcgtcca aaggggctct caggcaggtt
    35881 gtgcaacccc ttgtttccgt tactgaagaa atcggggacc ccggcgacaa gaatatttcc
    35941 gagatcaccc atgactccag gaatgttatc ccaggggctc tttttgcctg tatcaggggt
    36001 ggaaagcaag atggtcacct ttttgctaat gaggccagaa aaagaggggc cagcgccctt
    36061 ctttgcgaaa atgatacagg agaggaaatt gtccacctga tagtggatga tgccaggaga
    36121 agagtcgggg aagttgcttc ggcagtttac ggaaatccgt cctcatcgat gaaaatggtt
    36181 gcggtaacgg gaaccaacgg gaaaagcact acttctttta tgcttcgttc catcatgggt
    36241 gcctcagggg aaaaggcagg gatagtggga acaattgtct atgatgatgg ttctggaata
    36301 gagatggagg ccaatagaac cacacctgaa gcaaccgata tccagcgatt gcttttaaat
    36361 atgtcgcaaa acggatgtaa ctcctgtgtg atggaggcat cctctcatgg tctttcacaa
    36421 tatagactgt ccggttgtct tttcgatggg gcggtcttta ccaaccttac tcccgagcac
    36481 ctggatttcc atggaacaat ggaaaattat tttgcttcaa aggtcaggct tttccaggat
    36541 cacatgaagg ggtcgggatg gaaagtttca gtaaacaatg atgatccata cggcagggtt
    36601 attcgggaga tgttcaaagg taatgtcctg tcttttggtc ttgaaaaaga attttcccct
    36661 gattatctgg gtgagatcga ggggatggat ctggaaaaga tcgaaatgac agtttatggt
    36721 cctttcaatg gttcccccat aagattgaaa ctgcctgtaa cgggatcctt taatgcccga
    36781 aatgctctcg gggcggcgtc gctttctttt gcgatgggtt attctgcgga aaatatcatc
    36841 aagggtctcg aaaaccttcc ccaggttccc gggagacttc agagagttct ccttcctggt
    36901 ggagttaccg cgctcattga ttatgcacac acaccggatg ccctttttaa cgttctttcg
    36961 gctgtcaggg aagtctgtaa tggcgagata tggtctgttt tcgggctagg tggagacaga
    37021 tttcatgaaa acaggcctgt aatgggtgat attgcttcaa ggatcgcaga tcaccttgtg
    37081 atcaccatgg acaacccaag gagcgaggat ccggcatcaa tagctgatca gatagttgcg
    37141 gggataccct caaaggatga caaaaaaaga ggcgaccggg ttataatcct tgatcggaaa
    37201 gaggcagtgg aatatgccct tgatcgggca gcaacaggag acgctgttct tatttccgga
    37261 aaaggtccgg aaaaaaacat cgtcttttcg gacagggtta ttccctacag tgatcttgag
    37321 gcggtagagg aatggttgaa aagaagaggt tcgaaatgaa caggttttat cagtgcgatg
    37381 aatggactct ctcgaaagcg gcgaaactga cagggacacg atggtcgggg gaagacgttc
    37441 atctttgccg gcctttcagg gtcgacacca gggaaatcca ggccggggac gtatttattg
    37501 cactgccggg ggagaacagc gatgggcact ttttcctgga tgacgccatc gaaatgggag
    37561 ccggagccct tgttatcaac cccgccctta caggtcctgt gatggacagg atactggatg
    37621 agaagatacc cgttttgttt gccgacaata cagggcgggc tctcaaggaa cttgcccgta
    37681 aaagacttga tatactgaaa cctgaatgtg ttatggcaat aacaggaacg gttgggaaga
    37741 caactacccg cgaaatggtg cgtgccgtaa cggaaagccg tggagatgtt cattgtgcca
    37801 gaaggagttt caatacctgg atcgggtgtt ctcttaccgt gctggacgcg cctccaggga
    37861 caaaggttat catattggaa atgggcacaa atcaccccgg cgagatatct gaaatggcga
    37921 cactttttcg gcccgattac gggatcatta ccgaaattgg agagggacac cttgaaggcc
    37981 tgggagatat tgaaggtgtc ctttcggcaa aaatggaact tgccgagaag ggagaactca
    38041 aggttctgtc ctataatatc gataacaact cattgtccag atccgttggc tcccttccgg
    38101 caaaaatcaa aagaatctcc gttggaagga gtgattccca ctacaggatc atttcctcca
    38161 cttttggcct tgtcaaagaa aaacccctgc tctctgtgga ggttatgact cccaaaggct
    38221 tgagaaaagt aagtgcagag cttttcggcg atcataatgc cctgtcaatg gcttttgcga
    38281 tggcagcggg agattatctc gggatcggac ctgaaattca ggtcagggcc ctcgaacagt
    38341 tcaatcccat gccgggcagg ggcgtcgttc gacttctacc gggtgggggt attggcatcg
    38401 acgataccta taacgcgaac cccctttcga tgacacaggc ccttcgcaca atttcatcta
    38461 tgactccttc ccctggatcg aaaagaatgg cagttctggg agggatgggg gaactcggag
    38521 acatatctgg aatgcttcac cagaatatag ccagcctttt tgaaggactt gacgaggttt
    38581 atctttacgg ggaaacctgg aagacctcga tgaagggacg ttttccttac aacacaaggc
    38641 atttcaatga catagaggag attgccacag ttctccagga aagggtgttt cccggggatg
    38701 ttatcctctt caagggctcc aggtctttca aggtagaaag agtgatcgag atcctggagg
    38761 gtacaagatg atgggaagtg tcctgttttt tgcaggtgtt cttgttttgg gtgtaatttt
    38821 gcaggaattt tgggtcaaat tcctgcaaaa aagaaatata tcacagacca tgaaggagta
    38881 tggtcctcag aatcatattc tgtccaaaaa aggaacacct actatgggag gcgtgatctt
    38941 tcttttcctg ggcgtggtta tctcttttgt gttttgcctg gcagggttct ggaacacaag
    39001 acttgctatg gaaataatgt tctttcccat tgcaggaggg ctagtagggc ttggggatga
    39061 tcttctcaag tattacaggg tttcgagcga ggggttaaaa agtcttcaaa aactggttct
    39121 gcagtttatc gtggcagttt tgtgggctgt gttttttttg tcatggagag atctctctct
    39181 gtttcctgga tatgagatcg ggggaataac agcctttctt ctgattgtct tcttccttac
    39241 aggggggctt aatgcggtta acataaccga tggcctggat ggtctcgcat ccggagcctg
    39301 tgccatatct tttgcagcct tcatccttct tgcgggggcg ggaaaccctg cttttgccgg
    39361 ggccctttcg gggcttgcaa tttcaacggg ttttcttttt cataattctt ttcctgcaag
    39421 ggtcttcatg ggtgattgcg gttcccattt tctggggggg ctgcttgtat cggtctgtat
    39481 ggcagggaat ttcaccctgc ttcttctccc tttaggtttc atcatgggta tcgaggtaat
    39541 tacggtaatt atccagatag tggccattaa aggttggggg aggaaggttt ttcttatgag
    39601 ccctgtccat catcatttcg aacttgcggg atggagtgag aaaagggttg tctggacctt
    39661 ctggggagct catattggag gtttgttgat aatgtcggtt ccggtcatga taattgcgga
    39721 caggttcggg ggagcatgaa atgaagggat cctttgaaac atctgtcaaa gctcgcaaaa
    39781 aggatttttc ggtcacagtg gtcggggcag gtgtcagcgg gaagtccctg gcaattctgg
    39841 ccagaaacct cgggtacagg gtatttgtaa ccgaggcgag agatatcgat gaagaaacgg
    39901 tcggatcatt caggaactac ggaatatttt atgaagaggg agaacattct ccaaaaatgc
    39961 tggagtgtga tcttgtggtc ctcagttcgg gtgtctcacc aaaaagcgag ccggtggtta
    40021 tggctaaaag ccggggtata caaataatgg gagaagcaga ttttgtatcc ccttacctga
    40081 agggaaagat catcgggata accggcagca acgggaagac cacgacgaca atgcttaccg
    40141 ggcatcttct tgaaaaggcc ggtttccgga caggggtggc aggaaacgtc ggaagtcctc
    40201 tcgccgaaca tgccgggatt gactatgatg tcgttgttgc ggaacttagc agttttcagc
    40261 tttactggac aagcgatctg cggaccgatg tttcggttgt tacaaatgtt gaccccgatc
    40321 atattgactg gcacgggtca cttgaagagt atcacaggtg caagagaaaa attctcgcga
    40381 tgttaaggaa tgacgggaaa gtgatttgtc gcggtaaaga tcttgaggct ctttacccgg
    40441 aagggttcgg taaagaagga ttgtaccccc ttttttggga agaagatgga tctctatatg
    40501 acaggggcca tatattaatg ttgtcagagg aagcggaatt ggcaagcgga gagggcaggt
    40561 ccgttctttt ccgctataaa gaccttcccc ttctggggag acataaccat gaaaatgccg
    40621 caatggcagt tacggctttc tctatgtttg ccaacgattt atccaccgtt tcggcctcac
    40681 ttgagaattt caaggcacct ccccacagat gtgagctggt cgcagtaata aagggggtat
    40741 cctatattga tgattccaag ggtactaacg ttgctgccac ggtgactgcc ctgaagtccc
    40801 tcaaggggag aaagacagta atattggggg ggaagggtaa aggagaagac tacgctcctc
    40861 ttgcttccgc tgtagccagt gaagcagcct ttgcgatcct gatgggagat gagaaggata
    40921 atattcgcgc ggctcttgac gaagtcgggt ataaattcta tcaggaggcc gaggacatga
    40981 atgatgcggt agaaaaagcc tctttatcga ccagggaaga aggaggcatg gttctccttt
    41041 caccggcctg taccagttgg gacagatatc ccaactataa agagagggga atggatttca
    41101 gaagaaacgt tgcaaaactt gtggaaagga acatcgatgc aggctaaagt ggatcaaatt
    41161 cctgccaggc atagaccact ctgggggctt gccattcagg gcacctgggt gatccccctt
    41221 gttctctcat gcatcgggat attgatgatc tcgtcggtaa ctgcccaatt gacagtaacc
    41281 cagggcagga cacccttcta ttatggttta agacagataa aatggcttgg tgcaggtctt
    41341 gtcatgatga tagcctgcac tgttcccacc acggagttct ggaaaaaatg gagtggagtc
    41401 atctggctgg gatctctttt tcttatggtt tttaccctca ttccaggtat tggcagggaa
    41461 ggtggcgggt ccaaccgctg ggtcaggttc ggtccttttt cgatacaggc atcggaagtt
    41521 atgcttctga cctttgccct tcatcttaca aaaaagttac gtgaggctgg attagacaga
    41581 aaaaaaggta tggcaaggac tatcgtcctc tttgtgttgt catcgtggcc ttttgcgttt
    41641 cagcctgatc ttggcggagc attgataatg tttgtccttt ctatgggtat ctatgttcag
    41701 gtttttggct ggtttcttcc gattgttgca ggaatttccg ctatgatagg gctttttgtt
    41761 ccctttgttt ttttgagcag atacaggttt gaaaggctta tggcttttca tgatccctgg
    41821 aaggaccctc ttgggagcgg ttttcagacc ctgcagggct ttgtggcttt tgcaaacggg
    41881 gggggatttg gtgttggtat agggcatggt ctgcaaaaac tccagtacct cccggccgcc
    41941 cataccgatt ttattcttgc tgttataggg gaagaacttg gacttgcagg aacatttact
    42001 ctggtgatcc tttttggagt tttttttctg atacagtttc tttcttattt gacactggat
    42061 gatgattttg acgcgaccct tgtctgggcg atgacccttg caatttttat tcctttcatg
    42121 gtgaatgccg gtggagtcct gagaattctg cctctcactg gagttcctct tccctttgtg
    42181 agttacggag ggagttccct tgttcttgga tggtgcaagg ttggtatcct gctccgcctt
    42241 ttgaggaacg tttagatgaa ggtgcttctg gtagcaggag gtactggagg gcatatatgg
    42301 ccggcgatag cgttcgcttt atggctcaat gaaaaaaatt cgggaaacga taccagattc
    42361 gcctgtggga gcagatccct tgagagggaa atattcgcgc atgagggcat agagcccctt
    42421 ttccttccga tagagggatc tcccctgtgg ggcgatcttc gaacaaggtt ttcgagatgg
    42481 agaggtatgt ggaaatcctt ctggatctcg gccggtcttt tgagggaatg ggaaccggat
    42541 gtctgtattc tcttcggcgg atatgtgtcg tttccagtaa taattgcctc tcttgttaca
    42601 gggacacctt ttgtggtaca tgaacaaaat gccagggcag ggagggtaac caggacagcc
    42661 tccctggcgg gttctcctgt tttctccgga tggaaggaat gcctccctct tggtgaaggc
    42721 aaatttgtcc gatcgggtat tccggttaga tctatgacaa ggatggcccc ggaaattgcc
    42781 tggcgaaaac tgggccatta cgaagaagtt cctccttcac ccagagtgct ggtgcttggt
    42841 ggttcccttg ggagtgatcc tgtatcgcaa atctttgcgg aagcatcaac aatggagccc
    42901 tttagcggct ggagcattat tcaggtgagt gctgtgaagg aacccgttaa gagaggggtg
    42961 aaccattgga ttcttcccag agaatgggat gttggccttc tttattccct ggccgatgtg
    43021 gttgtttcca gagcaggagc atccactctt gcggaaattg ttgaatttgg cctcccttct
    43081 gtcataattc cgtggagagg agctgccgac gatcaccaat ttgaaaatgc aagactcttt
    43141 aaagaacaag ggcatggttc catttgggaa ataaataacg gacttcttcc aaaccttggt
    43201 ttcatgatag actatgagtt gaaaagggct cggcccggtc aagaaacttg tcccgaatcc
    43261 ggcaattttg aatcttgtga aatactctgg agatatgtcc aacaatacgc agaagggaga
    43321 ggtcgcaatt gaacgacttg actggccgat tcgaagaact caaaaatgta catcttatgg
    43381 ggatcggtgg agcggggatg agcggtttgg cccttcttct ccggcagttt ggaatgaata
    43441 tcagcggatg cgatgtctcg catacgcatt acatacacaa gatagcgacc cagggtatta
    43501 agttttccct cggtcacgac aaaaaccacc ttgatgttta caagcctgaa gcggttattt
    43561 acagcagcgc gataccttct gatacggaag aacttcttga ggccaggaga aaggggatcc
    43621 tgacttttaa aagggcagaa gttcttagct ggctttttaa tatgagaaaa ggaataggaa
    43681 ttgccggaac ccacggaaag acaacaactg cctccatgac gggacttatc cttgaaaatg
    43741 caggtcttga ccccaccgtt gcgataggag gggaactttg cgatatcggc gggaacgcaa
    43801 aacttggttc cggggcccac atggtagcgg aacttgacga aagtgatgga tcatttgaac
    43861 ttttcagatc ccatgttgcc gttgttacta atgcggactg ggatcatgta gatttctacc
    43921 cgaatttcaa ttcggtcctt aaagcctaca agaggttcct cgaaaaccgc gcagaggaag
    43981 gtaccgcgat aatttgcggt gaggacaaag gattgtccat ccttctccag aacaatattc
    44041 agggcaagta tcttacctat gggtggggaa acggttggga ctggggagcc agggatgtca
    44101 ggcacactcc cggagggggt gtgtcgtaca cggtctgccg aaaaggttct cccgtctgtg
    44161 acattgttct ccaggtctcg ggagaacata acgtcctaaa ttctctggcg gcctgtgcgg
    44221 cagccgattc cgcgggtatt ccccttgaac tctcggcaca ggcattgcgc tctttcaggg
    44281 gagcaaagag gcgacttcag cacatgggtt ttatcaacag gatgaatgtc gatatttacg
    44341 atgattatgg acaccaccca agagaggtat ccgcaacatt gagcactctg aagaaaatgt
    44401 ttcccgacag aaggcttacc gctgtttttc agcctcaccg gttcacaagg acagctgcca
    44461 tgtacaggga atttgcggag gtactctcac ttgccgatgg cctgtttctc cttcccgtat
    44521 tccccgcaga cgaaatcccg atcgaaggag tttcctcaac gcttattgga gatcttgtca
    44581 tgcagaaggg tcacggcgaa tacgccctct gcaacgatat ggatgaagtt gtcaacagga
    44641 tatgctcctc cgtaaaagaa ggagatgtaa ttacaaccat cggagccgga gatgtttcca
    44701 ttgtcagcga aaaaatcatg cagcgccttg agaggaaagg cgtgaccctg gatgcggtgg
    44761 cggtcaaggc ttagcaagga aattcaggcg tttttttcct tcgatcaacc cctcagtgga
    44821 atgacgaccc ttggagttgg aggccccgcc gagtgtcttg ccagaccaaa gacactcggt
    44881 gagttctgcc tttttctcga tttttgccgc tcagagggaa ccccgttgtg gataataggt
    44941 agcgggagca atattcttgt tcctgatgac gggttgcccg gtataactct tcaaaccttc
    45001 ggactgaatg ggatcttttg ggaagagcaa ggggactttg ttcttcttta tgccgaatca
    45061 ggagttctca tggcatcgct tctgggcctt tccatcagga aggggtggtc cggcctggag
    45121 ttttccgcag gcataccggg aactttgggt ggagctcttt ctggcaatgc gggggtgggt
    45181 gatctgtcta tagggggact tgtagagtcc gtgggagttg tcgatacgaa tgggaaggtc
    45241 gagacaagac aggcagggga ttgtattttt tcctaccgtt attccagtat tggagaagaa
    45301 ggaacaccta taattttttg tacacttcgc cttgaaaggt ccagcccgga tcacgtggct
    45361 gacggaacaa ggaaaatgct gtcaagacga aagggacaac caaagggagc tcgtacagcc
    45421 gggtgcattt ttaaaaaccc cccggggcaa agcgccggta tgctactgga tgaggccggt
    45481 tgcaaaggga tgtcttgcgg aggtgccacg atatcccgaa ggcacgccaa ttttattgaa
    45541 aactccggtt cagcgactgc cggtgatgtt gcctccctta tcgtgaaatg tgccgataga
    45601 gtgcgtggtc gcacaggtat ttcgcttcag agggagatcc ggttcctggg ttatattccc
    45661 catgaaaaaa ttaattacta gaatcattct tgttttatcc tttattgccg ggatgttata
    45721 ctcttctgag agcttttatc aatttttcag gtttcgggga gtatcgtttt attcgctggc
    45781 tcttgtttcg gatgatcttt tctggaaacg gattgatcct atttcccaca ggttatggcc
    45841 atttttaatg atccggtcca gtaagatcga gaaatcgcta atgtccgttg cgccggtttc
    45901 agtggacatt gaaatgacat cgcccggttt tttcaaggtg gattttttgc cacttgagcc
    45961 ttggttcctt gttttctggt cgggtggaga atggtttctt tccagggaag gaaggatgtg
    46021 gtctgttcat cattccctta acaatataat ttcagggcag gaggcaatag aaggaccggt
    46081 gcttgtttgg ggagaggatc tttccgaccc tgtccctgca gggatggatt ttgaaaattc
    46141 cgttgttgat tcgagcttgc ccatgggaga aatcctttcc tggaaggacg cgttggtcaa
    46201 gaccggtttc tatccgaggg tttcctctgt cactgtcaga aaacgcgagg gaagacggat
    46261 aattgagatt cttgaaagga ccgggtcagg tagcataagg atactccttg ctgatacgac
    46321 tgaaaactgg aaaacactct tcgatgctgt tgacgagatt ctttcgcagc cgggagtaag
    46381 cgggaaaaac ctggtgctcg atacaactta taccggcaga attcttgtaa gggttatttc
    46441 ctgaaataaa ttttattaag gaacggggtg ccagacatga gacctgaacc agcggatcct
    46501 ttcctgaatt ctgttcttga tatcggtacc cgcaagatag ccgttgcggt gtttgagagt
    46561 gatccggcca caatggaatt tcatgttaac tgcattgcca cggcacattc aatggggatc
    46621 agaaagggtg atattctgga tcctgatctt gttgtcgaat ccataaggca ggccttcgaa
    46681 aaagctgaaa aggaagttgg cctccatatc agggatacgg tggtgtcctt tgggccgaca
    46741 ggtacaagga caattgtttc ggaatataaa atttgtctta gtgacgaaac ggaacctgaa
    46801 aagcctgtta acgcaagcga catgaaagag gtggtcgcca aagccattgc ttcagagtgc
    46861 cccagaccgg aagaatttct gatgcatgct ttcccgatgg agtattctgt cgacggggga
    46921 cagcccctga aggatccgcg gggagtaaaa ggaactgaac tttcagtttc ggttctttgt
    46981 atctttgttc ctgaacagtc tttcctggat gcgctggatt gtgtcaggaa atcaggtgta
    47041 catgttcatg gaataatcca taaagctgtt tcctctgccc tgggcctgct ttccaatgaa
    47101 gaattcagga gaggagcggt ggtagtggac atgggtgccg ggacaacaag tattaccact
    47161 atacgtgact cccggatcag aggcgtatcg ctattaccga cagggggaga ccatgtaacg
    47221 ggggatgttg ccgaggttct ggggataccc tctttcgcag cggagttcct gaagcgtgag
    47281 gtctcccttg aggaggagga agactctctt tccgacgaac ttgaaatcga aatcgacggt
    47341 aaaccttttg ttacaaccgt cgaagaggtt ctttatattg tttcttcaag atttgaggaa
    47401 atgatggtcc ggtttgttaa accgggtatc ctttccatgt cggcagggaa ggggccaatg
    47461 gaggtcctct tttcgggggg ggtgacccgt tcgcccggtt ttattgatat gaccgaaaat
    47521 cttactggtt ttcggtgcag gatagctgag ccggtcaatt ccagaagttt gcctcccggc
    47581 aagggggggc cggaatttaa ctccctcata ggctcaatcc gctatatttc cgagagagaa
    47641 gagtcgccgc tggattacct tgaaccatcc ttcgaggctt ataatgagga ccccgatgat
    47701 cggtcccaga tcgacagaga tacttccgca atatttggat caagagtaaa acccttcaga
    47761 agcgggaaaa gagccaaaaa aaacagatta tcaggccttg cggatgccgt aaggaaagct
    47821 ttcagggaac tgttctaaat tattcccgtc cggaggtatg accaatgccc gaactttttg
    47881 agattgaagg ttcccagaga cagattcaag aggttatcaa ggtcgtcggt ataggtgggg
    47941 gaggaggaaa cgccctgaat cacatcgtca gaagcggtgt aaggggtgtg gaatttattg
    48001 ccgccaatac tgatattgcc catctcagcc tctctgaggc agacctcaaa atagtgcttg
    48061 gcagggaact caccagaggc cttggcgccg gcgcggatcc cgagatcgga ctgagggcag
    48121 ctgaagaatc cgtcgaggaa attcgcacta ttctcgaagg gaccgatatg gttttcctca
    48181 cagctggaat gggaggtgga acgggtaccg gcgcaacgcc tgttattgca aagatagcga
    48241 gagagagcgg tgcccttgta gtatcggtag ccactaaacc ctttaatttt gaggggaaaa
    48301 aaaggatatc ccaggcgctt gaaggacttg aaaacctgag agaccatgtt gatgccctta
    48361 tagtgatacc caatgaccgg cttctggaaa tttctccgaa aggaacaact ctctcggacg
    48421 cttttaaact cgcggacgag gttcttcggc aggctgtaca gggtgtaacc gacctgatcc
    48481 ttcggccggg gctggtcaat gtcgatttcg ccgatgtcag gaccattatg acccaggccg
    48541 gaaccgctat catgggtatc ggggaaggca agggcgagga cagagcgtcc aaggcggccc
    48601 atcttgctat caacagtccc ttgatggatg gaccgataac cggcgccagg ggtgtgctat
    48661 tcaatgtaac cggtggccca aatgtgggaa ttcacgagat ccaggaagcg gcggaggtcg
    48721 ttaccagggc agccgactca caggcaacta tactttgggg tcatgtgctt gacccggata
    48781 tggatgaaaa gatccagatc acagtcatcg ctaccggttt ttcgccagag ggcatgagaa
    48841 aaaaggctcg cacctttatg gatgcaccga taagggaaga caccctggca gatttccaga
    48901 gtgcttcagg agatgacaaa acattggcct gtgatccctt tgaagatctg gacagacctg
    48961 tagtcaagag gaagccggta tcccgtaatg aataaaccgg acaggggaac ggcatttttt
    49021 gtccagtcgc cgaggtaaaa ccggtcgcgc ttacggcttc gccggggcca gatacggtga
    49081 agtgatactt gacaatgtgc cttccggagg ttcgccttcg tgggcaatgc tttatcccgc
    49141 ggaatatgct gtagggatgg ccaacctcgg ttttcagagt atctgcgctg aactcaggac
    49201 aatgggggtt ggagttgaga ggtttttcca ggaaaaagga acccctcgct ccgttgaaag
    49261 ttgcagaagt attaataatt ttccctttgt cagtgcctca attgcctatg aaccagacct
    49321 tgtttcaata attaatattt tttcacagtg gggtatatct cccaactggg atgagagaga
    49381 aagcagggga gatccggtat tcggggttgg gggagctctt tcctatataa atcctcttat
    49441 cttttccggg attgctgatt acgtggttct gggagacggg gaaccggtcc ttgaatacct
    49501 tgtaggagag tgccggaaat atatgtccca tggggacaga cacagactct gggaaaaact
    49561 gtcagaacat ccgtcaatat ttgttcctcc cctgcacagg gaccggattc tttccggaaa
    49621 gagtacagga aggaaaaaat cctttgtcag cgacatggat agcgtgcgtg ggaaaagcct
    49681 ctgggtgtcc ggtgattctg ttttcgggaa gacgatcctt gtcgagctgc agcgtggctg
    49741 cagaaggggt tgcagctact gtacaatacc ctcttccttt ggccctgtga ggataagatc
    49801 tctggaaaag gtgacagggg atatcgccgg tttcaaggat tcagggaacc tccaggtcgg
    49861 acttgtcact ccggaggctg gcgactaccc acatattagg caactgcttg aatatctcag
    49921 gtctgccgga caggcggttt cttttgcgtc actccgagtt gataatatga ccgaggaaat
    49981 gatcgaagcc ctggttcgaa gcgggcgtta ttcggtcact gtagcaccgg aggctggtaa
    50041 agattggctt cgtttcagtt gcgggaaaaa tttttcaaac gaccttatta ttgaaaggct
    50101 ttccatggca agaagggtcg gtgtcagaca ggtaaaactt tattttatga caggattgcc
    50161 aggagaagaa atggaggacg tggacgcgat cccggaactt tgcagggaga tccatttaca
    50221 gacaggactc aaggtcaggg cctccatcgg gcttttcgtc cccaaaccaa tgagcaggtg
    50281 ggggatggag ccgatctgtt cccctgaagc tgtaaaagca aggttccgaa ggctaaggac
    50341 ccttttttca aaagatcatt cccgaggata tttggcaagt tttcaagatg agaacgaatc
    50401 tttcctggaa cattccattt cctggttcgg tgccgggaac gggtcttttc cagggggatt
    50461 gaacagaaga gcaatgagag ccataataaa ggaatacgtt acagataaag aatccgtgcg
    50521 ggaacagctt gaaaatctag gtttttaggg aaagcatcct gccagtgagg tgataccagg
    50581 tgagcttccg gaagaccaga aagttcaagg agaaacctgc catatttgct tttggacata
    50641 ttatgctgcc cctggtcggg atactggctc ttgggcttct tgtgcttggc gtgagacttc
    50701 tttttgtaaa tcccggtggc cagatctcat atccggaacc ttcccggcct caagcgtcac
    50761 agccggatcc cgagccggaa cctgaaatcg tggttgcgga tcagcaatcg gagatagtgg
    50821 ctgttcccgt tgttgaggac actgatagca gcccttcagt gtcactcaag caggataacg
    50881 aggtcgcgaa ggcatcaaag cccagccaga ctccggcgac aaagccgacg gcaacaaatc
    50941 ctttgccttc aacagacgtg agagcaggca cgtgggtagt ccagatagga gcatttacat
    51001 tgaaagaatc agccgaagca ctcgctaaag aagcaagggg gaaaaaatac gaggcctttg
    51061 tctttcaggc agaggttggc ggcaagacat attacagggt tagagttgga gcaggttcac
    51121 agaggaccga tgccgagact ttagccagag accttgcgtc aaaaggatat cccactcttg
    51181 tcacaaggca tgagtaggtt ccatagcctt tttcctgtaa tcttctttca ggaggtccac
    51241 agatgtcggg ttttgtaacc cggacctttt cacgtggaga agccatcgaa aaaattgctg
    51301 ggctgatggg cttcccctgg gtttttgagc tttcagaacc catacctctc gaaaaagcca
    51361 tcggttaccc atcaggagaa gaccttattt ccgaaacccc aaatccgcct tttaacagaa
    51421 gtctccgtga cggatatgct gtttgctcag aggatctgac tggtacaagc gaatcctccc
    51481 ctgttttttt gacactctgc ggcgaagttc ccatggggaa actcccggaa ttcaatattt
    51541 ctcgggggtg tgctgctctt gtccacaccg gaggggcaat tcccgaaggg gccgatgccg
    51601 tagttatgat agaggatact tccgagagtg gctcagctgt agagattcgc aaaagtatcc
    51661 aatctggtga gaatgttctt attgaaggag aagaattcct caccggccag gttattgtgg
    51721 agaaaggtga aattttcaat ttcagaaata taggctcagt tgcgtcctgc gggtttgcca
    51781 aggtgaggct ggtggatctg aagatcgccg tgataagtac gggggacgaa atagtccccg
    51841 tcggaatccc ggaacttctt cccgggatga tccgtgattc caactcctcc gttatcatgg
    51901 cgcatctttt gcgggccggg ttcaggagcc gttttatggg gatagcaggg gatgatcccg
    51961 gcagactggg cgaccttttt catgaggccc tggccggaaa tgatgttgtt cttcttagcg
    52021 ggggatcctc ggtcagcgta agggatcatt ccgttgaact tttgcgaacc cttggtgagt
    52081 tcgagggaga tgttcctgta aggggactta atatttcccc agggaaacca accatagtat
    52141 ctgggaatgc ttcaaaaaaa aagatcgcgg tatgtcttcc cgggcacccc cactcctgct
    52201 ccgttatatg tgccactttt cttgtccccc tcctgagaag cgcggtaagg ggaaagcagg
    52261 aagaactttt caaaaagatc agacttcccg cggcttccga cataatcggg aaaagcgggg
    52321 tagaggagtt cattcccgcc cgcatcgaca gtaatggtga agccctgccc ctttggggca
    52381 aatcgggcta tgttatggcc ctgaattcct ccgacggttt tatcaggcta tcagaacagg
    52441 aggagacggt caggtgcggc aagaaagtcg aggtgtggct ttggtagtct ttcccgaaaa
    52501 tatttcgctg gaaaaggcat ggtcaataat tgaagaaaga gtcaggtcgc tggaaagagg
    52561 cgagatggtg gaaattgcgg atgttggtgc tccttacggg aggatccttg cgtccgacct
    52621 gtttgcaagg tttaatctcc cccattaccc ggcttcggcg gttgacggct acgccataaa
    52681 ggctgtcgat acggcaagag cctcgtctac ctctccgtcc cggatggaac ctggttcgta
    52741 catctgggtc aacaccggcg gaggggtcga gcgaaaatat gacgcggttg tgatgataga
    52801 ggataccttc atggacgggg agttcctaat ggttaccaaa atggtttccc agggggacaa
    52861 tgttcgacct gaaggggaag acgtcgccat ggggcaggtt attgcaagaa aaggggaggc
    52921 ccttaatccg ttgaatttac cgctcctgat agcctcgggt tattcttctg ttcccgttca
    52981 acctcttccc aggacaatct tcctgccaac cggagacgaa atagttgaaa tcgccagatg
    53041 gatcaatggc gggtacggag aatcggacca ggctccggaa acaaattcgt ggatgctgag
    53101 ggggcttttc tctgaatggg gtttcccgct ggatgtcggg gagatccttc ccgatgatcc
    53161 cggaataatt ggcggagccg ttgaaaaggc tctcggcgat tacgacctgg tagtaatagg
    53221 agcgggaact gccaaaggca gaagagatca cagcgccgga atcatatcca ggtttggaga
    53281 gccacttttc agaggtataa ggatgaaacc gggcagaccc gttatagcgg gtatctcagg
    53341 caaccgacca ctggtcgcgc ttccgggttt ccccatgtca gctcttgtta ctgcctggac
    53401 gatcctgtat ccgatgctat gcaggctctc agggcaagct ttccatacga atatccagaa
    53461 agcgatcggg tgtcataaaa catttgaaac aagccttctc aggcatcact catcccagca
    53521 gggtatcagg gaatggatga gggtcaaatg cggagaaatt gacggtaccc tgtattcctg
    53581 gccccttgca tccggggcaa gttccatgtt ttcgatctcc gacgccgacg gggttgcgct
    53641 tcttgatgag tcgacactgg aactcccaaa gggaagtcgg ctgaatgtct ggatgaggaa
    53701 cgaccctgac tggaaacgga ggatcgttta ccagggatcc aacgatccag ccatagagat
    53761 acttgtctcc cacatgagag atcatggagg agatatggca ataaggtcag tcggaagcct
    53821 tggaggcctt gcagcaatat caaggggaga gggccatgtg gcttcgtgcc atcttctaaa
    53881 ccccgaggat gggacgtaca acagtgcata tatcaacggt ttttccggtt ccggagattg
    53941 ggtaaggatc aggcttttcc gccgggaaca ggggctgata gtatacaggg gcaacccttt
    54001 gggaattggc tccgtaaaag atctatcaat cacgggagcg tttttcgtaa accggcagcc
    54061 cggggctgga acaagggtcc tttttgacca cctgcttaaa gaaaatgata tcgtttcggg
    54121 gaaaataaaa ggttacaata gtattgccgt tacccacctt gaggcctcgg caagggtggg
    54181 gagtggttca gcagacgcga ctctcgggat aagggcagcc gctgatgcct ttggaaatga
    54241 ttttgtgccg gtaaccatgg aagattttga gctcataata cccgggcgtt tcatgaacca
    54301 tgatggtata tctattttga ttgagaccct tgatgacgca aactggcgtt tggaagtaga
    54361 aaacctgggg ggatattcct ggattatttg aaggacaaat ttggcagaag gctaaattac
    54421 ataaggatct ctgtaaccga cagatgcaac tacaggtgtg tctactgcat gccagaagag
    54481 ggtctggaat ggatacccca tgaccggata atgacgtatg aggagatagt gtttctttcc
    54541 tctgtacttc atggtcttgg tgttgagaaa atccgcttta ctggaggaga acctctggtc
    54601 aggaagggtt tttttgattt tctcaaggac ctggctgata aagttccaga tatccgaacc
    54661 gttctcacta caaacggtag cttcattgga gaatatatcg aggagatcag ggaatcaggt
    54721 ctttcgggaa taaatgtgag ccttgattca ctgaatcccg ccgttttcgg gatgataacc
    54781 aggaaaggac cccttgagtg tgttctaaag gggataagaa ctctttccca ggaaaccgat
    54841 attccgatca agatcaacac tgtcctgatg aagggtgtca atgacgaaga aattccggaa
    54901 cttctggatt ttgccatgga aaacagggct accttgagac ttatcgaatt tatgccactg
    54961 gacgattcgg tatggaggga agatcttttt atcccggcct caagtatatt tgaaaagctt
    55021 cccaggccgg gatcctggat aaggatagac aattcatcag atcacagcgc tggacctgcc
    55081 gtatactata aaaacgaggc aacgggccag aagataggta ttatagaagc agtcactcaa
    55141 catttctgcg aaaaatgcaa caggctccgg atctcggcga caggtattct caggccttgt
    55201 ctcttttcgg aggatggtat agacctcaga ccttctctta tttcacgtga ccccgcactg
    55261 gtcaggaaga accttatgca gggagccctt gataaacctg aatgctatgc tgcttcgaag
    55321 acaggcaaga tgcacatgtc gaggataggt ggataaaatg ggacagtttt cacacatgga
    55381 cgactacgga catccggtga tggttgatgt gggaggtaaa ccccatacaa taagagaagc
    55441 agtggctgag ggcctggtaa gactgcccgt taaagtaatg gaagccatta tgacaggagc
    55501 cgctgtcaag ggagacgtcc tccgggtggc cgagatggcc gggatcatgg gatgcaagag
    55561 aaccccggat cttattcccc tttgtcaccc cataaaactt gaccatgctg ccgttgaatg
    55621 ttccgtagac cctgaaaaga gactggcacg gataatctgc aggacaagat catgcgatgt
    55681 tacgggagtg gagatggagg ctcttacagg tgtctctgtt gccgccctga ccctgtacga
    55741 tatgtgcaag ggaatagaca agggaatggt aatagaaaac ataagactcc tccgtaaaga
    55801 tgggggaaaa tccgggagtt atattgcgga ggatctaaaa tgaaaagcag agttatgggt
    55861 ttttccggat cgcttccggc gcaacccgtc gtttttttac acgaggatag cgagcgcaga
    55921 cctctggtta acggcaaagc gctaaaaatt accattcttg aaccaggcca gaagaagagt
    55981 ggagatgatt tttttgtcat cagggcttcc ggtttcggtg aagttgggcc tggggatttt
    56041 gtatcctttt cggagggtgt tatcctccgt atggatccgg caattcacga tggttcgatg
    56101 tttgttgtca gacccggctt catttcggtg tcttcaaccc tggaggtctg gtcatcgatc
    56161 caggcgggag tccttaccgt cagtgacaag ggtagccgtg gagaacggat agatacttct
    56221 ggtccggcac ttgaggcagc tcttttgggg atcggagtct ctactctcat gagggaaatt
    56281 gtgcccgatg atgcagaaaa gatctccagg atccttgggg agtggatatc ttccgggaaa
    56341 attccccttg ttcttgtaac gggagggaca ggactttccc ccagagacgt tactcccgag
    56401 gcgctcctgt cgctgggggg gaaaattgtc ccgggcctgg gtgagtttat gagatggaaa
    56461 acatcattcc tgaacgagag gtccattctt tccagagcct ttgcggttgc ctgtgaaaaa
    56521 accctggtta tatcactacc cggaagcgaa aagggagcaa ttgagtgttt cagctcaata
    56581 tcttctgtcc tcagacacgc actggagatc ctttccggga aaggaaccaa ttgcggtagt
    56641 tcccactgat aaaggttttt taaaggaggg atcagacaga tgaaagagtc cctcgcaaac
    56701 agagtctcaa gatgcatgat aatttcagga atgtccggcg gagggaaatc caccgccctc
    56761 cacatgttgg aggatgtggg tttttatgcg atagaaaata ttccccccaa cctgcttcca
    56821 caactgatcc aggtactggg caagcatgag gaagccgtcc agaatggtgt tgccgcggtc
    56881 gtagatgttc gtggtgaaag tcttcttgat cacctttttt cagtcatcgg atacctcaag
    56941 aaaaaaggtt tggaagtcgg cgttatcttt ctggatgcct cagattccac tttggtaaaa
    57001 cgtttcgaag agacaagaag aaggcatcct ctttcatgtg attcaaccat tctggaagcc
    57061 attcaaatgg aaagagttga actgtcaaag atccatgaga tcgcagatgt cgtagtggat
    57121 acatccaaca tgtcactggc cgaactcagg cgggagatac tcggaaagat cgagatatcg
    57181 gaggggccct tatcggtgat cttcacatcc tttggattca agcatggaat acccccagac
    57241 agcgattttg tctttgacgt cagatttttg cccaacccct tttacgaacc atcacttcga
    57301 tcgggatccg gaaaggacag gaaaattcag gaatttattg cacgttcgcc ggatatttct
    57361 gctttcatag aaaatctttc atctctgctt gggtgcattt ttggacttta catgaatact
    57421 ggcaaaaccc aggttcacat agccatagga tgtacgggag ggagacacag atcggtggcc
    57481 gtagcggaat ggttgggaga gaagttcccg gagaactctc tcgtaagaca tcgtgacatt
    57541 gaaaaggata cggtgcttca atgaactggt ggtccgcatt tactgttgga cttctttccg
    57601 ggggtattat gtccctcctg gcggcaagac tttttcagga ggaagaggaa gataactctt
    57661 ccccgcaggg aaaaaacggg acggttatgc ataaggcatt ccggcacaag gtttcccagg
    57721 gtccggagat cgttgccata ggtgggggaa caggtctttc taccctcctt tcggggctca
    57781 agtcatacac cagaaatatt acggcgatag tgacggtaac cgatgagggg gggagttcgg
    57841 gccgcctcag gggcgagtgg ggtgtgttgc cgccaggaga tataagaaac tgtatggtcg
    57901 ccctggccga gaacgacagc tccctgaacc ggatacttaa tttccggttt gacaaggggg
    57961 aacttgaagg tcacagcctt ggtaacctga tactgctggc ggcaacagag atgaccgggg
    58021 atttccgcaa ggctgtcgag gagctgaaca acctgcttgc tatcagaggg aaggttatcc
    58081 cagtaaccac agaatcggta acactttgtg gttcaacagg agatggcaga cagcttcgtg
    58141 gggaactgaa tatctcaagt tgcgggaaca ggttggactc catatggctt gaaccgggtg
    58201 atgcaagggc ggttccggag accctgaagg ccataagcga ggcagatgta gtgattctag
    58261 gtcccggaag cctttttaca agcattgtcc ccaaccttct cttctctgat attgccgttg
    58321 cactcagaag atgcagtgct cccaaggtct atattgccaa ccttatgacc cagccaggcg
    58381 agaccgaggg aatgaacata atcgatcacg taagatggat aatccgcata tcggggatca
    58441 cacccgatta cctaatagcc agtgactccc cgatcccata tgagatgctg gatagttacc
    58501 gggcatcggg agctcagccc ctcgttcttg aggaagaaca taagaacgct cttggactta
    58561 tgggtacgag ggttgttgtt tccgactatc tgagtatctc tgaaggaagt gtcaggcaca
    58621 acagccagaa attgtcagag acccttgtga ggatagccag ggaagagagg gatacggcaa
    58681 aatggagaaa ctgaagtccg tggtctggga cgaatggggt actgtcccga acagcgggcg
    58741 ggagggggca acaatggaga ttgccggaat cctttccggt ctcccctcga agacagagaa
    58801 taaagtaact tcttttttca gtagccgtct tttcgtcttc cgaaggatcg ggagactctg
    58861 ggattcatcg gaatggtccg cctttgggga attctcttcc ggactggtta tacctcccga
    58921 cctgaaggga agggtttcca taagaataaa atccccagga tcccttgatc ttatgttccc
    58981 tttcaagaaa gggaacagga gtactccaga ttcgtggaac tggctaagag gtgtctttgg
    59041 ttcgagcgga tccctttttt tgccaaggac gggctattac atggttttcc gccttccgaa
    59101 ttccagtgag gaagatcttg ggaaagatat tgcgactata atgaggtacc gggacatacc
    59161 ctccaggacc aggtcgcgct acggttttcg ggaagttgct gtcagggatc agtcgtctat
    59221 agtgggaatg ctttcaaaca tgcggctctt caaaacatcc cttatacttg aagagaaagc
    59281 catgctcagg tctctcaggg accgggcaaa caagatcgta aactgtgatg cctcgaacat
    59341 tagaaagacg atcgagacag cggaaagaca gatcgtaacg gcgaggcaac ttatagattc
    59401 cgggaagatg gccaggcttc ctgagcccct gagggatctc attgaagcca ggatcgagaa
    59461 ccctaccgct acattgaagg agcttggtgg acagctcgcc aggccggtca gcaagagcac
    59521 agtggaatac aggtggaaaa agatagagaa gatagcggca agcgcaggcg taaaaatcca
    59581 accaacaggg ggatgatcgt catgtacctg ggaaaggctg atgtaaacac gtacgaaaag
    59641 ggctccagcc gggaattttt gacaacgaac ggctggggcg gatacggatt ctccaccgtg
    59701 atcggggcca atacccgcag agaacacggg cttctggtgg taaacaagag tggaaaagaa
    59761 gtgtatcccg atgtactgat tagcaaactt gacgaaacag ttatcagcag ggggaaaaaa
    59821 taccatcttt ccaccaacag atacacggac gtagttttcc ctgacggata caggtacctg
    59881 caggaatatc atgccgaacc cctgccgggg atgcttttcg tgatacatag tgtctttttg
    59941 aggaagactg ttttcatgcc acgaaaaagg ctttccacga tcataaaata tgaactcctt
    60001 tcggcaccgg aaaagatcga gctcgagatc aggccccttg cggctcacag gggcatagac
    60061 caggtatgct cccacaaaga cagggatttt tcttcgtcct tttctggcaa ttgcgtttcc
    60121 atagaaggga gtgatcttca gagtcgcttc tatcacttca atggagaatg gagaccgaaa
    60181 cctctctggt tcgagaacct gatctacgaa tttgacgagt gcgagaaacc gggaacggac
    60241 aacctctggt caccgggagt tctttccgtg gatatgtccg agggagatac tatatatatt
    60301 gttctgagca gcggggttga gaaatatgac cacggtatct gctctgaact tgaagatatt
    60361 actgtccgct ggatagcatc gaatgtttca gagactccgc tgatgaaaaa aacccccatg
    60421 gcaagggacc ttattcagtc ggcgtcacac tgtatttcag agattgaggg gggtaacccc
    60481 gtcatattta ccggatatcc ttcagtccgt gaaaaagcca gagagacctt tatatccatt
    60541 cctggacttc ttttgtccac gggcaaaata gaatttgcca gaaatcttct tgaagtgtgg
    60601 cttgaccgtg ccgcaggaaa cgggaacgta atgccctctg ggatagaccc tcatacgggt
    60661 aggttaacca gtcaatctgc cgattcgggt ttatggtttt tttatgccct tggcaagtat
    60721 tgtgacagga gcgagtccac ggcattggcc gaaaaaagat ggggcgatat aaccgctctg
    60781 gcgctgaaga tcatcgaggg tatacccgag atcggcctct ctcttgataa agacggcttg
    60841 cttgatctgc gatcggagag tatcggtagc aactggatgg atggcagcaa taacggtacg
    60901 gccattgtgg agaggcatgg aaaactggta gagtttaacg ccctatggta caacggtcta
    60961 aggactcttg agcaattggc cgagttgacc ggcagggcag atcaggcccg agattttggc
    61021 aaaatggcag acctctgcgg aacgtctttc aagaaagttt tctggaacga aaagggttac
    61081 ctttatgact gggtagacgg ggacaaaaaa agcgattcca acaggtgcaa ccagatattg
    61141 gctgcctctc ttccggtatc cgtcatggat cctgaaagaa gccgctcgat agttgaatct
    61201 tgctggaacg acctttatac cacattcgga ctccggaccc tggatcctca ccacgagaaa
    61261 tacaagggta gatgtgaggg tcgccttgat cagagggaga aagccaggtt tatgggtatg
    61321 gcctggccgt ggatgcttgg ccagtttatc accgcctttc tgcgttataa tccggacagg
    61381 aaggacatcg catggtgttt cctgagaccc ttcctctcgc acctccggaa gggctgtctc
    61441 ggtggagtgg ctgaggtttt tgacggcagc atgccataca ggccgcatgg aaacgctctt
    61501 tatgctccaa gtgtcgcaga acttcttcgt gtgatatctg aggatatgtg agatccagaa
    61561 aaatatcagt gaactaacca gactgaaagg attttcaagg aagggcatgg ccaggctatg
    61621 cccttttaca tgggacaagc cgcagtctgc ggaaaaattc aagatttgat aatggagggg
    61681 tgcacaatgc aaaagaagcg tgtagcaatt aacggttttg gcagaatagg caggctggca
    61741 ctcagggctc tttacgaata tgagaacgag ggcctttttg atatagtcgc gactaacagc
    61801 cgtacatccg ctgagcagag ggcctacctg ttcaagtatg attccgttca ccgccgtttc
    61861 agtggtgacc tgaagatcag cgaaggatcc cttgtgatca acggaaaaga gatcaaagtc
    61921 cttgcagaac cagagccaaa aaatctccca tggaaagaac tgggggtaga tcttgtcatt
    61981 gaatcatcag gaaaatatac tgatgccaga atggcagaag gacacctcag tgctggcgca
    62041 aagaaagtcc tcataacggc tccggcaaaa aatgaagatg caacgatcgt catgggagtc
    62101 aacgaacata tttatgatcc ctccgctcac catatcgtat ccaacgcatc ctgcaccacg
    62161 aactgtcttg ctccggtggt aaaggttctt tatgaaaatt tcggtataga agaaggactg
    62221 atgacaacaa ctcattccta cacaaatgac cagaaaaccc ttgattcttc acacaagaat
    62281 tttcaccggg gccgagctgc ggcactttcc ctgataccta ccacaaccgg ggcagcggct
    62341 gcgataggaa aggtttatcc tcaggtagaa gggaaaatga acggtatcgc ggtaagggtc
    62401 ccaacaccgg atgtttctct tgtcgatctt gtagttcgtc tgtcgagtcc tgccacaaaa
    62461 gaaacgataa acggagcttt caaaaaagct tcctccgggc ctcttgaaaa atatctgggt
    62521 tacgaggagg acgatcttgt ttccgtcgac ttcactggag atagtcgttc ttcaatattt
    62581 gcggccaggc ataccatggc ccttggaacc atggcgaaga tcctggcctg gtacgataac
    62641 gaatggggtt atgcctgcag ggtcatagat cttgcaaatt acatgctggg taaggaataa
    62701 ggtgacattg cgatggctct ccgtaccttt gaaggagttc ctcttgaggg tctgaaagtc
    62761 cttgtcaggg ttgatttcaa cgtaccaatg aaaaacggaa agatcaagga tgattcaagg
    62821 ataatagctc acctcgagac aataacggcc ttgaaaaatt ccggagcctg tgtggtgctg
    62881 atctcccacc ttggaagacc aaacgggaaa gctgatcctg ctttttcctt aaggccagta
    62941 gccgaagcgc tttccattga gtatggtatc gcctcgggtt ttgtggcgga ctgtgttgga
    63001 agggaagtag cttcctccgt aaaggctatg aggccaaggg atgtattgct tcttgaaaat
    63061 gcaaggtttc atcctgagga agaatccaac gatccatctt ttgcaaaaaa actggcggag
    63121 ccctttgatt gtttcgtgat ggatgctttc agtgcctccc acagagctca tgcctcaacg
    63181 gaaggcgtaa cagcctggct cccatcctat gcagggagag taatggaaaa ggaagtatct
    63241 gtcctttcca ccgtaaggga aaacccgaca aaacccctgg ttctggtcat gggaggcgcc
    63301 aaggtttcag acaagatacg cattatcaaa tacatgatcg acaggactga ttcaatcctc
    63361 gtagggggag caatggcctt tccttttctg aaagcgctgg ggaagaatat cgggtcctct
    63421 ttctgcgaag aagggacaga ggagacggca cttgaaatcc ttgaaatggc cgaaaaggca
    63481 ggggcaagga tcaaccttcc agtggacgta atagtctctg attcaatcga aaattcccgg
    63541 aattcgactt gtgtgtccct ttcctccata ccaaataata tgatgggtct ggatattggt
    63601 ccgaaaacag ctgaacaatt ctcagtagtc atttcaggcg ccaaaacggt tctctggaat
    63661 ggtcccatgg gggtatttga gacaccccct ttcgggagcg gaacgaagac tcttggggaa
    63721 tcggtagcaa aggcgacttc aaagggaact ttgtctgttc ttggtggtgg tgacacggca
    63781 gctgcagcca aggccctcgg attttcagac ggaatcagtc atatttcaac tgggggaggg
    63841 gcaaccctgg aatttttcga gggaagggac cttcccggaa taaaacctct ggaaacagcc
    63901 tgaccgatct catggcgagg aggaaggacc catgaaagag cagattttat tggccggtaa
    63961 ctggaagatg aacaaggata ttaaaggatc cctctgcttt atcgaagaat tccaggaaat
    64021 ggcagaaaaa gaagaccttt ttgggaaaac tggtaaaaaa tttgaaatgg cgatttttcc
    64081 ccaaactgtg aatctgtatg cgttgaatca aaaaattctg gacctgaagt taccgattct
    64141 tcttggcgct cagaacatac attgggaaag atccggagcc ctgaccggag agaactctgg
    64201 ctcttcagtt ctggaggcgg gggcgaaaat ggccctggtg gggcatagtg aaagaaggca
    64261 tattttcgga gaaactgacg aaatgacagc aaggaagtac gtttcatgcg ttgaatgtgg
    64321 tctcaggcct gtattatgcg ttggtgaaac ggaagaagaa aaaagcaggg gattcaccga
    64381 aaaagtgctt tccaggcagt tgagaagtgt ttttgccccg gatagatcat ctgaagaatg
    64441 gggtgaaata atcattgctt acgaacctgt ctgggccata ggaaccggga aaagcgcaac
    64501 ctccgaggac gcggcaggct cgtgtgattt tatcaggaat ctgttaaagg gtatttgttc
    64561 cgaaaaggaa tcccaaaacg ccatgatact ttacggaggg agcgtcaaga gttccaacgc
    64621 tgcggccttt atggaaaatg gaagcataaa tggccttttg gtcggaggtg cctcacttga
    64681 tcccttcgag ttcatgggta tcctcagggc ctgtggtgtc taatgtaaca taagatatat
    64741 tataagacac ctaatcgaac ctttaaaccc ccttaaataa gggggttttt tatatcaaac
    64801 tgtctggttt gctttgattg ttctggcaga agcccatatg ctttcgagac ggtaaaaatc
    64861 tctgccttct cttccgaaaa tgtttactat aatgtctccc gcatccagga gaatccattt
    64921 gggactgttt tccccctcga tacgataacc gacattcatg ctgtcaaggg tgtccgtgac
    64981 atgacccagc aaggctttca tgtgaacatc cgaattaccc gttgcaagaa cataagcatc
    65041 agctactgta gattctctgg aaagatcgat tatggtgatg tcctcagcct gtttgtcctg
    65101 aagaatctgg actatcgggg cgaccgaatc ccctttaata gaagccttca tttaacccct
    65161 cccctatctt gccgaagatg aattgaatct ttttaataca tccatgtaat cgtgacccat
    65221 aataattgta acacggctta tagaggtgtc cttttttaca aggttttgtt gaatacctgt
    65281 caacttaccc agggcttcag ccgctttctg gttgttttct ccaggggggt aaaggatgtt
    65341 cgtatgcctg tagtcgtagt gtcttgcgtt ccctatgtga cccacatcaa ttcccactga
    65401 ctgaaaacgt gtagctgctt ccttgccgat accagcagtt cctgatccgt taaggactgt
    65461 gaccggtaaa cctatctcaa taacaagttc ggaaataagg gcaggatctt ctgtatcggt
    65521 ttgttctcct gcgggatttt cttccgggat ctccgaaaag aactcggaaa gggtccccgg
    65581 gtctccaagc cagtaactta ttccggaaat gaaggccgcc ttacctggaa gagttcgaaa
    65641 aactgttctt gaaggatcca tgtccttcag gtaggaggca agttgtatag attgggagag
    65701 agacatgttt gtctttacga tcttcattga gtattttata agttccggaa ttctggttac
    65761 cattcccggt tccttgatac gggcaaaggc cgccttcaga aagatctgct gtctctcaac
    65821 acgtccgata tcacccaggg catcatgtct gaaacgaaca tatccaagtg ctgtctttcc
    65881 gtccatatgc tgtactccgg caggaatatc gataaaaagg ttcccggccc tgtcggaata
    65941 cttgagacgt tttggtatat ccagggttac cccgcctatc atgtccacca gctttgggaa
    66001 gctttcgaaa ttgactatca catggtaggg aattggtacg ccaaggtagt ttacaagggt
    66061 agccttcaaa agttccactc caccataagc gtaggcgtgg tttatcttct gccatccgtg
    66121 tccgggtatc tgaacacgtg tgtccctggg cagagaaagc agccgtacaa ttttttcatc
    66181 gatgtctatt gatacaaatg cgattgaatc cgatctgttg gcagtctcgc ccggaacttc
    66241 gtcaagtcct acgaaaacca tgttaattct tcctgtaact tcatcgtgct ggattttctc
    66301 cctgatagta ttgggagccg gattaaagaa aaaatatgct ttcagtccca cacccgccac
    66361 aagagaaaca aaacacgcaa gggcgatcag tgccatgttt ttcctggtca atcttttcac
    66421 catccgtttc tgaaatacat agcgtttttg taaatatatt gctcaacaag ccatggggcc
    66481 aggtacctga tattaaggcc gtcggagacc ctttttcgga tctccgtgct ggatatcgcg
    66541 aggagaggta tttcgagatt gatcatgttt tctctgatct cctcggggag tctggctatt
    66601 tccccgggat ccgaaccagg tcttgttacc gcgactatct tgcaaagttt ggggatctcc
    66661 agaggttctt tccagttcat caggtccaga acagcatcaa gacctgtaat aaaataaaat
    66721 tcaacgctct ttgggggata ccagtggcgc atctccctaa gtgtatcaac tgtgtgactt
    66781 ggagtttccc tgtctatctc aaccctcgat atcctgaaat gaggattgtc gagggtcgcc
    66841 agcatcgtca tggtgtacct gtgttcggcc gaagatatga ccgttccggc cttatggaag
    66901 gaatcacccg tcgggacaaa aacaacttcc gtgagaccta gagagaaata ggcctcttca
    66961 gccgcgatga ggtgtccgaa atggatagga tcaaatgttc cccccattac tccaattttc
    67021 cttttacgca aaccctctga cgatcttacg ggcaatttca gtccctaccc ttcttcagaa
    67081 gaagcctttt cctgattggg gggagactcc ggaataaagg taaactccag atctccaata
    67141 ataattgtat ctccttctct cgctccgtgc ttaacaagga ggtcttctat cctgtagtgc
    67201 tgcatgagag ccatgaatct tcccagagcc tcgtcctgat cgaagttgaa tcgttttatg
    67261 agtctttcta tttcaggatg aaagacctgg aataatccat catttcccgg attttttata
    67321 acgggcttaa tttttttaag atcttctgta aaaggacttt cgatctcgaa gaaacggctt
    67381 tctcttttgg ttgccggtac cttgtgtatg acggatacaa tatgtccgat aaatgcttct
    67441 atcccttccc ctgtgaccgc gcttgtttca aagaatggaa tttccgcttt gtaaagttcc
    67501 tccctcaggg cagaatatcc atccttgtcg cggggaagat ctgtcttgtt tcccactgcg
    67561 gcagaaggaa aactcagaag ttccgggttg tattcgagga actcgttcct cacggtattc
    67621 cactgctcca gtgggggtgc cgaagcgcct tcagagagat ccataacgta aacaataagc
    67681 cttgtcctct caatatgacg tagaaatgca tgtccaagtc ccctgtcgag gtgagcgcct
    67741 tcaatgagtc cgggaacatc ggctataact attttttcac tttctacggc cagaatacca
    67801 aggtttggcg aaagagtcgt aaaagggtaa ccggcaatat ccggtctggc tccagagata
    67861 gccgatagga gcgatgattt tccggcgttt ggaagtccca caagcccgac gtctgcgagc
    67921 attttcagtt caaggaggaa atttcttttt tctccatccg cgccgttctc ggaaaaacgg
    67981 ggaattcttc ttctggaaga ggcaaatctt gcattccccc tcccacccct gcctccgcgg
    68041 gcagcaagga aagtgtcacc gtggacagtc agatccgcga aaatcgttcc tgttccgggg
    68101 tctataacaa gggaaccgca agggacagga accttgacgt cctctcccga ggctccgtgc
    68161 ctgtttcccc cttttccgtg agaaccatca ccggcagcaa accgccttcg gtattcgaaa
    68221 tcagcaaggg tctggatgcg atcatcggct acaaggtaaa cgttgccccc cctgcctccg
    68281 tcgccgccat caggccctcc cttcggaaca aacttctccc ttcggaaact cagagatccg
    68341 tttccgcctt ttccggattc aacccatatt tcagctacat caatgaatct caaggatcaa
    68401 caccccgcaa aaataagaaa aaagggccca agaaatactc aggccccgga aacacaaatt
    68461 catcaatata gttatcaggc gattacgttc tctatagcaa cgaatttcct gccggccttg
    68521 gtgacaaaac gaaccttgcc aggtttgagg gcgaaaaggg tgtcatccct gcccctgcca
    68581 acgttgtctc ccggatggta ctttgttcct ctttgcctga caagtatgct tccggcggtt
    68641 acttcctgtc cgtcacttcg tttaatgccc agtctctttc ctatgctgtc tcttccattt
    68701 gtgctgcttc cctgtccctt tttatgagcg aacagctgca ggtcaaaaat tttcatttac
    68761 atacacctcc acgattttcg tgtaagcagg atatgacttg ctaattgaca caagtgccgc
    68821 gactattgtt tttgcaagat cgtttattct gctactgtca ttgttgcccc agaatattct
    68881 ggcgtatcct gatactggat cttttgtgga aaccacattg tctatgtcaa gtacatcggt
    68941 aaggccaatt tcaagtgtct gtgccaacgt agaaagggcc gcgcagacaa tgtctttgcc
    69001 atagccggca taaccggaat ggccggagat ctctatcccg cagataatat caccgcaacg
    69061 caggatacgg atctcggtca tcgatctgtc aatactcgat cgagtcaatc aatacggaag
    69121 taaaggattg cctgtgcccc tgtgttttcc gtgactgctt tgatttgttt ttgtacttga
    69181 agacaataat ctttttatcc ctgccgtgag tctctacccg ggcttttact acggcacctt
    69241 caacggttgg tgagcctatg gataccttgt ggtcggatgt aaccatcagg accttgttga
    69301 attcgatggc cccaccctca tcgacgtcta tcttttcgat cctgatccgg tctcccggtg
    69361 ataccctgta ctgctttcct cctgtttcga tcacagcgta cataagtgct tttccccctc
    69421 ccgccggaac ctcgggtggc ccttaggctc tttaaacccg ttccgagcgt aattatgacc
    69481 catcgggtca attacgttat ttgccggtca cccccggccg aaaaagaatt ataacataaa
    69541 agagaacaaa taaaaccggt ttaagtcaat tattcctggc cccaagaagt gagctggcca
    69601 gtcttgaggc ctcaggatca tcgggatttc ccgccagcat acgagatatt tccagtaccc
    69661 tcgagtcatg gtcaacctcc ataaccctgc tggtatcccc ttccctttcg accttgaaat
    69721 gctggtccgc cagagctgca atggaggctt cgtgagtgat cagcaatacc tggcaccttt
    69781 tggacagttc cttcagggcg tagccggcaa gtaccgctgc cctccctcca aggccagcct
    69841 cgacctcgtc aaagaccaat acatcgggga gcatctcgtc tgatgaagcg atctgaagag
    69901 aaagaaggat cctgctcagt tctcctccag aagctgtttt ggcgacgggg ccataaaggt
    69961 gcttgtcatc ggtcaggaaa aatgaaactt cgtcggcacc gttctgacga accttctcca
    70021 gaggattaag agatattccg aaacggaggc cttccatagc gaggtttctg agggtcgagt
    70081 ttactgcttc ctcaaggttc agagcagcct tgcggcgcat ttccctgaga gacaaagcaa
    70141 gggaagatac ttcctttttg tatgaagtcg attcctcctg aagttttgcc agcatctggt
    70201 tgctctcttt aagccaatca agccctgctt tggtcctttc agcgtattcg accagatcat
    70261 ttacattatc ggttttggca aaacgcatca gttttctcaa catcccgaga cgtgtttcca
    70321 gatcatcgaa atggttctgc agctcctgtt catttgaacg acagacaatg gtttttatca
    70381 catcctcaaa ttcctgtgca gccctcaata aattttcgct tgcgccgtaa aggggttcat
    70441 cagagggggc agcgattact gattcgcggc agatgctctc taaagaggaa aggattccct
    70501 ctcccgaact gcctcctgtc agtctgtcgt gaatgctttt catttgtttc tggttcctca
    70561 gagaggttga aatatcgttc atagtggttt cccattcgga gaatgaactc tcttccagct
    70621 tgagattacg gtaccttgca aggaatgatt ctccgtcctg gcaggctgcc tcggtttctc
    70681 tcctgcgtct gttaatttcg atcagttttc tttctgtctc gagtgcaaga gcaactgtcc
    70741 tcgagagcct ggacctgatc tccttcagtt cgggcccccc gctgttgtcc agcatttcaa
    70801 cctggcgtga aggttcaaga agaccaagtt gggcaaactg gctctggatg gcaataacgt
    70861 tggagagagc cgaatttacg atatttaacg gtactggtct ttcctgaaga taacttctgg
    70921 ccctcccggt agatgagaca agcctcctga tcaccataaa cccctcctgg ggctgaaggt
    70981 catcgggcag ggaaggtacc gggccgtcca tctcaagaac agcctgaacc tctgcctcat
    71041 ctaccccggc acgtatctgg gcggattgcg ccctttttcc gcttgccagt tcaagggctc
    71101 tgacgaggct gctttttcca gccccgcttt ccccagtaat ggcaataaaa tttcccctga
    71161 aattaagggt tgcatccttt atgccgccaa tgtttgaaac gattagctca gaaagcatcc
    71221 cgtcactcct cgtcgctctc ggcgatcctg tggccccact ggaacttttc ctgaaggagc
    71281 gaaaaaaaat cgtttccctc aagggcaatg atcccgaccc ttctggaggg gtcgagagta
    71341 actgttattc ggtctcctgg gagaagttca taaccgagct ggccgtcctg agtcagcatg
    71401 atctccctgt gctgcccttc aggtgttatg gatatcgtgt ccgaatcact cagtaccatt
    71461 ggtctggaat aaagggtgtg ggcgcaaata ggcgtaagga tcatacatga tagatgtgga
    71521 ggtatgatag gaccccctgc cgacagagag tatgccgtag agcccgtcgg tgttgagatg
    71581 attatcccgt ccgcatgaag aacgctaagt ggacatccgc ttacggcgat attcagggca
    71641 ataagtctcg caaaagggcc ttttgtaacg acaagatcgt taagggcata aacttcgtgt
    71701 ttctgacgct tgcccctgct gactaccccg tgaagcagtc gtcggtactg aatattgtgt
    71761 ttgccctgga ttataatttc gagatctttc tccgcgttct cggcttttcc acaggaaaga
    71821 aatcccagat gacccatatt gatcccgtaa agggggatat cgtatccaat aacgtatctt
    71881 gcggccctca ggaaggttcc gtctcctcca agaacaactg ccgcgtcaac ccgttctttc
    71941 caatccttgt ctgaaatctc ctcaaccccc aatatatgtg cctcatggga agggagagca
    72001 aaattgacgt tcctttttcg cccccagcga agtatttttt cagcaagatc aagggctggc
    72061 cgtttctggg tatttacaag aagacccaat gttgtcatcg aataactcct ccggtcttca
    72121 tgaattcagg gccccaaaag ctttgaatgg gcctctttta ccatatcctt tatctgttca
    72181 tttcctcggg acgtaaggag acccgccgag gtgcttttaa gatgaatcag gaactctata
    72241 ttcccctttg ggcccaatac aggagaataa tcggcgcccg cgtagaaaag ggaactgttg
    72301 ttctccagga aatccagaat tgaaacaaga acagcgacgt gactctcgaa gcttctgact
    72361 actccttttt tccccacagc gcttcttccg gcctcgaact gcggttttac gagacagacg
    72421 cactctccac cctggtcgat aactctttcg acagcaggaa taacaagcct gagggatata
    72481 aaagaaacat caactgaaac aagatccggg ggtggtttca gatcgccggc ctctaggttt
    72541 ctggcgtttg ttctttccat caccactact ctcggatcct gcctgagttt ccacgcaagt
    72601 tgcccgtaac cgacatcaac agcataaacc gttttggccc caagggaaag aaggacatcg
    72661 gtaaaacccc ctgtagaggc accaatatct gcgcataccc ttcctagagg atcaataccg
    72721 aagactttca gtcccttcaa aagcttgtgg gctcccctgc tggcccagac aggtccatcc
    72781 ttgaccctta tatcagaatc atcactgacc cttaatcctg cagtggtggt tttttgtccg
    72841 tcaacggtca catccccagc cattatcatt gactgcgcgc gagtccgact ctccgtgagg
    72901 cctcttttga ccatttccag gtccagccgt tctttctgag gtgatggcat ggtatttccc
    72961 gatcacctcc tccgggtcaa gtccacaatg tttccactgc tccccgacag ttccgtgggg
    73021 tacaaattca tcaggaactc ccatagtaat aaccatggac cggaacgggc cttcagtggc
    73081 gatagcagat atcttttctc cgatcccgcc ctccctgtag ccgtcttcaa ggacaataac
    73141 gtaattgtgg gcagaaagca tttctcggat catatcaatg tcaaggggtt ttagaaacct
    73201 catatcgtaa accgatggag gcagtaatcc cttgctgata gccttttctc gggtgatacg
    73261 ggccagtggt aaagtggaac catatgcaca taaagcccaa cctgaacctt caaccagttt
    73321 ttctcccttc ccccatggag cggttacgtg actcctgttc ctgtattcag cttccggtgc
    73381 aataccccgg ggaagcctta tggcgcaagg cgacattttt ttggcggcgc cctcgtacat
    73441 ggccctcaga tccgggatat ccctgggagc catgaccgta aggtttggta tcgatctgca
    73501 ccaggaaaca tcaaggattc catgatgtgt ctctccgtct tccccgacga gcccggaacg
    73561 gtcaattgcc agaatgacag gcagattttg catgcagatg tcgtgtacga gctgatccat
    73621 ggccctttgc agaaaagttg aataaataga gacgacagga agcaagcctg ccgacgccgt
    73681 tcctgcggca aaggtcagga ggtgtccttc ggctatgcct acgtcaaaaa atctttccgg
    73741 ataaatactg gaaaacctgt tgagtttgct gccttctttc attgctgccg tcatacagac
    73801 aattcgaggg tctttttttg ccatatcctc tatgcaagaa gcggaagcgc tgctccagca
    73861 gagggatttt ggcctatcgt ttactgacac ttgtattttg ggagatactc cgtggtaagt
    73921 ctccggcata ttttccgccg gttcgaaacc cttgcccttt tttgtaagta tatggatgag
    73981 cagagggaat tcgtaactgc gtgcaaggtc gaatattcgc tgcatctctt ttatgttgtg
    74041 tccatcgaag gggccccagt aactgatgcc tatctcctcg aacatgttgg gcgtaaggag
    74101 gaatcccttt atcttctgtt tgatcttttc aaggaaggat tcgagtcggt tgcccctggg
    74161 aagttttctg cagaaatatt tcaggaaact cttcaggtta tggtaaaagg gactgacgct
    74221 gagagcggca aggtggttgg ccattcctcc caccctgggg ctgatcgaca tctggttatc
    74281 gttcagaaca tatataaccc tggttcctgc ctcatgagtg ttgttcagcg cctctagagc
    74341 catgccgttc attagcgatc catcccctat gaccgcaaca acatgatgat cctggcccag
    74401 aatgtccctg gccttggcaa aacccaggga ggcggaaagt gatttgctgc tgtgtccagc
    74461 attgaaatgg tcgaaaacgc tctctttcgg gtccggaaat cccgaaatgc ccccccattt
    74521 tcgcaatgaa ggaaaacggt tttttcttcc ggtaagaatt ttgtaggcat aagcctggtg
    74581 acctacgtcg aatataatct tgtcccgggc agggtcaaat gaacggagaa gagaaacgat
    74641 cagttcgaca gcaccaagtg acgaacccag atggccacca ttctcggaaa ccgtctcgat
    74701 aatcagttcc ctgatctcta aacagagttt atcaagttgg ttgttttcaa gatcagcaat
    74761 gtctctgtag tctctgattc tttcgagaat ttctctcata aaatggtcgc tcctgtcgtc
    74821 cgttttccta gatgctcctg tcaagaagag tccctggcag tgaagcaagc agatcagtct
    74881 ccccattgac ccgcgaaatg gccctggaag ccagggaggt ttcccgggct gccaattgcc
    74941 tggccttttc cataccaaaa gcagcaacga aggtgatctt tccctgttcg gagtccttgc
    75001 cgggagtttt gcccaattct gatttgctcc ctgtaacatc gatgatgtcg tcgactatct
    75061 gaaaagccat tcccaggtgc tctccatatg cggaaaaggc agaacgggtc tcttcgtcag
    75121 tctcaccgag gaccaccccg gaaagcaccg aagccctgat aagggatgcc gtcttcgaga
    75181 atgcgaccct ccatgggtgt tcttctgttg aaagagtgct ttctggatct gaatccagta
    75241 cctgaccccc acaaattcct tttggtcctg ctgctttcaa caattctgaa acagcggcca
    75301 taatggtttc tggctgaaac ttatttcttt ttgcaagacc ctcaagagca aattcaaaag
    75361 cccagaggaa aagtgcatct cccgctataa gagacagtgg ttctccaaaa ataacgtggt
    75421 tggttggttt gccccttcta agaatgtcgt tatccattgc aggaaggtcg tcatggatca
    75481 gggaggctgt gtggaccatc tcacatgcca gtgccataac catggcgtca ttttttgaaa
    75541 ccccgcaagc ccccgctgaa agaagacaaa gaatcggtct tatccttttg cctccggcaa
    75601 gcagggaata tgtcatggat tcccacaggc gcaaaggaat attttctggt ctgcttccgg
    75661 atagctcaac cagggccgaa tcgacctgtt cccgataggg tgaaagaatc ttttcgaatc
    75721 ggttcttttc cataaccgat caggatcctt ctacttcttc ttcccgatcg tcctcaaggg
    75781 gattccatgg agagctctct gtgtcggcga tattctctga aagaagtgtt accctttgcc
    75841 tggccgattc gaggaacttt ttacaggagg atatcagggc cactcccctt tcaaaaaggg
    75901 caagggattc ctcaaggggc atatctcccg cgtccaggtt ttcaacaata tcctttaatt
    75961 cagctatgtc atcagaaaaa gtcatcccta aaaatcaccc ctgaattttt ttgactgggc
    76021 caatgataca acgaaagaga aacttgcgcc ttatgaatca accgtgatac aattctcgtc
    76081 gtttgctgaa catatcggac cagggaggta ttgctatggc taaagtttgc gattgctgcg
    76141 ggagaggcca ggctacagga aacaacgtga gccactccaa ccggcataca agaaggacat
    76201 ggaacattaa tttacagtct gtcaaggtgg atctggggtg cggcgaatcg agaaaattga
    76261 agatatgtgc ccgttgcctt cgttccggaa aagttaaaag ggctgtttaa gtacagccct
    76321 tttttacggc ttacgcacgt ataaaccaat ccatccttca tctattccca gtcttaaatt
    76381 tcccgtaaca gaataattgc ttactgcata aggcctgttt ttaagaatta cagcttcctc
    76441 caggggccac cttgtgcctt caaggcaaac gccaaggcat ctatcagaca gggcaagcaa
    76501 agatattagt gttcctgttt caagaccgga aacaccaaca tcttcacccc ctctcagaag
    76561 gagaagtgcc tctttgtcat cggcaatgca tctggtgaag gatctccact cctccccacc
    76621 ccatataaga gagaatatat ttgaaaaaag atgatcgaat cttcctccaa agcctgctgt
    76681 cagtatggcg ggggttccag gccacctgag acctttttct ctcagggcaa tctgaagatc
    76741 tgtaaggttt ttttccggag ggaaggtcat ggattcaatc tttttggact cggcccataa
    76801 aattccttct gcactcgcgc tatctctgtc tccgataaaa aggtccggta atacaaagtt
    76861 ttttcggcaa agatctattc cgctgtcgat ggcccaaacc cttcggcttt ggcaaaggtc
    76921 ctggaaccac cagggttctg gttgtctgcc tcctgcgaca agaacgaggg ggaagttccc
    76981 ttctcccttt gaaaatacag ctttgaactg aggtaattcc agagttgtgc ccattccctc
    77041 aacctctgtt gccgttcatg ctctcaaaga gaatgtttcg tgcctcatcc tcatcgacaa
    77101 gtatctccct gggtttcgag ccatcctgag ggccgacgat accgatctgt tcaagtgtgt
    77161 cgatcagcct ggcagccctt gtaaaaccta tcctgagttg cctctggagc ctgcttgcgg
    77221 aggctatccc gctgtgaagg atcatgttca tcgcctcttc aagcagatca tcgtccaggt
    77281 gtaccctgcc gttttcgcct ccgtcatcgc cctgaacgtc gatatcgagg aaaaccggat
    77341 caccgaaaag cctggaaagg tagtccatga atctctgtat ggtgggttca tcgatccatg
    77401 gtgactgcaa tctcaggggt tttggaagtc tcggggtcaa aaagagcatg tcaccactcc
    77461 caagaaggcg ctcggccccc gctacatcaa ttattgttct ggagtctgtt tgtgagggca
    77521 gggtgaatgc gactcttgcc ggaatattgg ctttgataag tcctgtaacc acgttgaccg
    77581 atggtctctg ggtcgcaatt ataagatgaa tgccagtagc ccttgccatt tgtgcgagcc
    77641 tgcaaatata atcctctacc tcgcgtggag atgtgaacat gagatccgcg agttcatcaa
    77701 ttactattac tatgtaaggc atcctgtcaa cagggaggac cctggtgttg taggttttta
    77761 tgtttctcac cctggcttgc gcgaacattt cgtatctctt ttccatctcc cttattgccc
    77821 atccgagagc gtgaaccgcc tgtctggatt ccacaacagg aggaattata ttatgaggta
    77881 gtttttcaaa ggatttcatt tccacccttt tgggatcgac cagcaatagc cttagatcct
    77941 cgggactgtt gcgtgaacaa agagctgcaa tacaacaagt gacgaaaacg cttttacctg
    78001 atcctgtagt tccagccaca agcagatggg gcatcttttc cagaccgata gaaaggtgtc
    78061 ttccgtctac gccgacgccg accggaattg cgagatcgtt gtctgattca agaaaggagg
    78121 ggtgttcgag tacagtcctc aggttaacgg gttttctttc aggattggga gtctctattc
    78181 ctatgcatgt ttttccggga attggtgctt cgaccctgag gctcggaaga gccagtgaaa
    78241 ctgcaagatc gttggcaagg ccggaaaccc tgcttacctt gatccctggg gcaagctgga
    78301 tcctgtactg gataaccgta gggccaacga cggtttccga aagctctgcc ccgatcccga
    78361 attcgtccag ggtggcaata atgctttccc cccgactctc aagaagatct ctgtcaaggt
    78421 cgaaaaaata atcgt
//
LOCUS       DAOTIQ010000003        61151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_464_length_61151_cov_6.699800, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000003 DAOTIQ010000000
VERSION     DAOTIQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 61151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61151
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_61151_cov_6.699800"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="P5541_00980"
     CDS             complement(<1..612)
                     /locus_tag="P5541_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRV70577.1"
                     /translation="MIERYSTQEMDVIWSQENRFRTWLEVEIAACRAWMEEGVIPEES
                     FLNILERSEIDVDRILQIEDSVNHDMIAFVSSVAEKVGPDGRYIHLGLTSSDVIDTSS
                     SLLLARSLDLILGDLGEVAEIILEKAWKYRDLACVGRTHGVHAEPLSFGFKLLNWYEE
                     IQRDITRLSASRVSVSVGKISGAVGTMPIARPRSRRGSVPFSVL"
     gene            complement(609..1130)
                     /locus_tag="P5541_00985"
     CDS             complement(609..1130)
                     /locus_tag="P5541_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRV70578.1"
                     /translation="MQPAVKRVAGILHPDTGLLEWTISKLTSEWGEIQYQSETYPFGH
                     TDYYRNIAPVLYRTFVSFSGLAGAGGLAEWKIFSCGLEKQSGDSRKVNIDPGYVNGSR
                     LVLASTKDHAHRIYLRDGIFAEVTLRYMRKKWVSFDCTFPDFAEGVYDSFLDEARKDW
                     LRETGSLGGVWID"
     gene            complement(1106..2050)
                     /gene="ftsY"
                     /locus_tag="P5541_00990"
     CDS             complement(1106..2050)
                     /gene="ftsY"
                     /locus_tag="P5541_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRV70579.1"
                     /translation="MPDIRNDLEPVRRRWNLFRLFACGMPEDSFWEDLEETLLSGDTG
                     VPLTLSIVEDLRSRIASSGGMDGEKALALLRDILCERLEAIDGTGEPVHFGGSATAVL
                     LLGVNGSGKTTTAAKLAFGLKEQGRKVILAAADTYRAAASEQLRIWGERAGTRVIAHP
                     PGGDPGAVVFDSMKAAAASGADCVIIDTAGRLHTNSNLMEELAKIKRVIGQNSHGWIT
                     ESLLVVDAVTGQNGFRQAEVFGRAIPLTGVVMTKYDHTAKGGVILSVGRELGLPVRYV
                     GLGEGLGDLQLFNPRDFVDGLLSDSKGGENRCSLRLNG"
     gene            complement(2074..3327)
                     /gene="ricT"
                     /locus_tag="P5541_00995"
     CDS             complement(2074..3327)
                     /gene="ricT"
                     /locus_tag="P5541_00995"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRV70580.1"
                     /translation="MSSYLMIFGKPRYLGLLENGEVTFSPGNRVVVESARGEETAVAA
                     GPITHEQAESYRVDLASTGGEGVPRGGEPSFQDLNFIRFATEEDLEARKAQEEEEEQI
                     LLKGRELLKSHDLEMKLVDVEFILDRKKLFFYFTAEQRVDFRAFVRDLAREFRTRIEL
                     RQIGVRDESKVVKGMSPCGMPCCCSYWLNSFAPICIRMVKEQNLALNPTKISGICGRL
                     MCCMGFEHNNYRELWKDLPGPGTKIKAPAANYIVSGVDINREIVRLYCPGNGEILVPV
                     SEFKYFRETVMKGETWTMESVVEPGSVNVSEEIVRIQEPVERESEDPECPGKETPGQK
                     QGEKRNQGKPGEKTGRSQRRRGGPNRKKNAQPSAKIVEDKKPSGGKEPGAETEKPVQG
                     KTESRKKSRRRRPRKPNSPTSGSNT"
     gene            complement(3299..4069)
                     /locus_tag="P5541_01000"
     CDS             complement(3299..4069)
                     /locus_tag="P5541_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70581.1"
                     /translation="MPSGNEVLSESIEKFIEETSSASAASDASPAFAVVLPLAYHADC
                     VMACARKILCDEMTREEQRPAWSEKDHPDLIQSGKPEEAATIEFCRTLWKELSLRPVS
                     STRRLGVLFSGNRMSLPAVNSLLKITEEPPPSGRIMILLEENSMLPTLRSRIRVFNFI
                     PGSSGEVEPPDIPGNPGTFLKWVGRTRKRSPQEMLPELENWVLFLARQKDFGRASSLD
                     SICMLARKGKLTTPMIQDLVWAGLEGEVRFEQLFDDFW"
     gene            complement(4128..4727)
                     /gene="tmk"
                     /locus_tag="P5541_01005"
     CDS             complement(4128..4727)
                     /gene="tmk"
                     /locus_tag="P5541_01005"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRV70582.1"
                     /translation="MTFEGIDGSGKSSLSEAVEVAIASVIGGERVVRTHEPGGWNGGG
                     ALRSLILSGSSENTWTELLLFLADRCEHVHRVIGPSILSGKVILCERFTDSTLAYQSF
                     GRGFPRDIIETFSQRAGFPIPDITFWIDLPVETALKRICERGEKPDRFEKDPGLLERI
                     SCGYRQIHESEPKRVIRIDGTLSPGVISSEVTSVILKRL"
     gene            complement(4749..6023)
                     /locus_tag="P5541_01010"
     CDS             complement(4749..6023)
                     /locus_tag="P5541_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV70583.1"
                     /translation="MNREMRVGIVVFLAIAFLGGLVFVSGGVRFKQYGYSFGIVFPDA
                     MGLDAGAPVLVSGVESGRVNSIALMDKGVLVSVSVKGHVIIPSDSKFQIDTGGLLGEP
                     RVKITRGPSFTSISPDDVVPGTIPPAFDEILEDIRRSLGDVQNTFSNINSFLGKLSDA
                     ADDFQGFSNEARGQLKRVGDSIVRLTGNLDSVVEENRESLSLSLNTLSEVMGKLRAVV
                     SQFDEDGMSGRDVRETVVRIGEAARNVGELTRKIEDTFFKEGEAPGKNTISDIREIVG
                     KANRIISDIEDIHFDGTVSVHGVTQGPRDSDVMADVSLWTGSRSRKIGLLLGAEDIGS
                     DPGVSAAIGYSGEMFRFWAGSVRGYPGVGFMLFPLHGAGGFSIGGQWWDQQNGRWSID
                     GRYSIGDSWGVFYRYLDLDDGHRDSAGVFYRF"
     gene            complement(6020..6790)
                     /locus_tag="P5541_01015"
     CDS             complement(6020..6790)
                     /locus_tag="P5541_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV70584.1"
                     /translation="MNSLMNMKNVSVGFSGKMVIRDVSIRLMSGEINCLMGPSGCGKT
                     TTLRLIAGLLKPQEGEINLLGKTIDPLTEEKELIDIRRELGVVFQGGALFDSMSVGQN
                     IAFPLRYCRNIHDEEKISSMVLEMLHHVELGDVREKFPSELSGGMRKRVAIARSLVHS
                     PSLLLLDEPTTGLDPITARHIDQLVVDLCGRFGFSVLVVTHDLVSALGIADRLMLMEE
                     GKIAWGGAVSDWHSSDHSSVKRFAEGMISVRGMGGIAG"
     gene            complement(6795..7574)
                     /locus_tag="P5541_01020"
     CDS             complement(6795..7574)
                     /locus_tag="P5541_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70585.1"
                     /translation="MNLVSWVGRQTVDLFSTLGEFALFGYRVICGTFVGRWSREDLLE
                     QLERVGVSSVLVVSITSAFTGMVMAVQTLDQFVRFGATGYIGGVIALSMIREMSPVLT
                     GLVVSGRVGAAMAAEIGSMKVTEQIDALRTFGLDPSVFVGVPRVLAGLIMLPMLTLFS
                     FVIGIGGGFAYVTAHGVNPHLFKRSVEALLNPSDIYGGVLKAAVFGLLITISACGQGF
                     MAGQGARGVGSATTRAVVWGNMLILIFNYILSTLLFGGIKG"
     gene            complement(7589..9373)
                     /locus_tag="P5541_01025"
     CDS             complement(7589..9373)
                     /locus_tag="P5541_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HRV70586.1"
                     /translation="MRKTWLFLVVAMILLSFPGVVYSGEFVVPGGIMGVDSLREGMQG
                     TAWTVVKGGEVVSFPVKILSVLPEPTSPRNLILIRASGPVIEKTGGIAAGMSGSPVFV
                     GGKLIGAIGYGWNFSEHEMGLVTPIEDILSIWDWPDRVPGIVLPDLELKDPDNDDPDG
                     SEDETPADLADSGDKMDRELSAPLVGMGYSARSAKRIEEILGMPVVMIPGDIRGNDIP
                     VEFGAVARPGEAVSVLVAWGDISLAATGTLTAVSNDGRFVAFAHPFTSRGAVSFPLAR
                     AWIHEVIPSYRSPFKLGTPRSIIGIVTQDRPQAIGGRIGQFAPVMDISVRFANVDSGE
                     RTLKRFKTAYDPFFMTKVIPEMLNGLIDNVWGRVGEGSAKLTMKIEGGGLVEGWQRTN
                     MFFSPTDLGKTMLAEFSMLSDAISLNPFFEIMPIGIHLDIEVTSDPRVVFIEDVNLSK
                     KTVLPEGEFDVEITLRPYRKETVKKKITLKAPAGARGVCEVVVRGGGIEEPGQDSIAQ
                     GWRAIQSLDELLEEFSAMETNDEVVAEIRSFVPGGDPESMEAEEESGRKLLSQIKEEM
                     IKEGSFRSYRSNYYVDGLIRRIIRVVPE"
     gene            complement(9399..10503)
                     /gene="prfB"
                     /locus_tag="P5541_01030"
     CDS             complement(join(9399..10430,10432..10503))
                     /gene="prfB"
                     /locus_tag="P5541_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583336.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRV70587.1"
                     /translation="MAQIPVSNVMEGLRSLAYELKDSLDPEKAAADLEKLTEATSEQG
                     FWNRDDAQDVTREIARFQAKVDSWKNIETELGDLQTLAGLLSEEDDSELEKEFQARSS
                     ELEKALEEKRLLLLLNEEYDDSNAILSIHPGAGGLDSQDWAEMIFRMYLRWAEERGFQ
                     SRVLELFEDDEAGIKSATIHVSGDHAYGYLKSERGVHRLVRISPFDTAKRRHTSFASV
                     NVSPELPDDAEIEIASEDLRIDTYRSGGAGGQHVNMTDSAVRITHIPTGIVVSCQNER
                     SQHMNRHSAMQVLRGRLYERMIQEKQEQMNHIQGEKKEIGWGSQIRSYVMHPYTLVKD
                     HRTNLEKGNVQAVLDGDIDDFIQSYLRFRKQGK"
     gene            10597..11616
                     /locus_tag="P5541_01035"
     CDS             10597..11616
                     /locus_tag="P5541_01035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRV70588.1"
                     /translation="MRKSTPTKTAVLKKLYSSIGETVSGGILCRELGLSRQAVWKAVR
                     SLVEDGFTIESVPVKGYRFLGSSGNDLDPTWLEILLSDCPWGHPVLFWQEIDSTQSPA
                     KEMARQGAPEGVVVLADQQTSGRGRLGRHWISPPGGGIYFSVIIRPDIPPEQIQTLSL
                     ASAMAVQDAIQSESGIRCQLKWPNDILWNNSKLCGILSEVSSEPGRIHFAVTGIGINA
                     NMRGQDMGLGKGATSLASILGHDVDRGLIAASVISRFHDGVRRLESPEGRISLISDYA
                     ARCDTIGKKVRVLVDEREIIGEACGIGDTGNLEVRSEGKILTFSAADVTHLRNCGDHQ
                     EDSLE"
     gene            complement(11595..12374)
                     /locus_tag="P5541_01040"
     CDS             complement(11595..12374)
                     /locus_tag="P5541_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70589.1"
                     /translation="MLFFCRSAVGAGSAEIWVTNPWLSMIARFIGGAHVTVRPLVVWN
                     DSGVQEKTRKEPPVGTLVFAVDNEEIIRFLGQGGLERPKTFRLFEKGFPGGNRDENFL
                     DPAALPFIALRLLDILSASEPSNYSYYQRRLAEFQARLDSTVIVGRNMIGSKSLLDLS
                     WKYGRWLQASAGKVVRPPDAVKNGWASGKGIEVLETALEEAVRQKWLIVTDPWTPGFI
                     RERVGKMPGSIELPRPDIDQDMILFLYEIYLQIWDYSRESS"
     gene            complement(12507..13094)
                     /gene="pyrE"
                     /locus_tag="P5541_01045"
     CDS             complement(12507..13094)
                     /gene="pyrE"
                     /locus_tag="P5541_01045"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRV70590.1"
                     /translation="MNDAVAVETMRRMKESGAFLEGHFLLSSGMHSGFYLQCALFLRY
                     PEHAAWAGARIGERIDHLRPDVIISPSVGGLIIGHETARYLGVPFIFCEREKGIMKLR
                     RFPAPLGLSFFVVEDVVTTGRSTAEVRDAILQVSEGEFLGAACVVDRSSGNNAIGPSL
                     VSLMKSDFPVYTPEECPLCGEGLPLEEPGSRRLSR"
     gene            complement(13091..13810)
                     /gene="pyrF"
                     /locus_tag="P5541_01050"
     CDS             complement(13091..13810)
                     /gene="pyrF"
                     /locus_tag="P5541_01050"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRV70591.1"
                     /translation="MLKKQKNRRVILAADLLDVDAALAMIEPLARELAFVKIGPGLFA
                     QGGMLFVKKVMEIGFDVFLDLKLHDIPNTVAMAVRQFADNGIWALTVHAAGGKAMLES
                     AAKTRDASGNPMKLFGVTVLTSHDLPSWEEVNPGCAMEEALRSRARICAASGIDGIVC
                     SPSDLELLRPIAEGNLEMVVPGIRDQAGQDDQKRSAPLEQALEKGADYLVIGRPILKS
                     PSPAIALSEIVDRVERWEQTR"
     gene            complement(13804..14751)
                     /locus_tag="P5541_01055"
     CDS             complement(13804..14751)
                     /locus_tag="P5541_01055"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRV70592.1"
                     /translation="MGNKRLETVVGGVRMETPVIIASGVWPMDPGLWPTGSMDGVGAV
                     CSKGITLYPRKGNTGGRVFETPSGMLNSIGLQNPGVSAFIDEDLGPLSSAGFPVIVNV
                     AFGSIEELDDTVHILEKSVDKIGAIELNVSCPNVSSGGMSWGTLPSSLEEAVAGARAA
                     WNGPLWVKLTPQAWSIADASKVCENAGADAVVVGNTWLGMAVDNQNEKPFFDRIFAGL
                     SGPAIFPLSLRMVWEAAGSVAIPVIGCGGVSGPDEALSMMLAGASAIQAGTSLFVESR
                     LPSLICRGLIEHMDSHSLENAVALTGRARPEKGAATQKC"
     gene            complement(14741..15556)
                     /locus_tag="P5541_01060"
     CDS             complement(14741..15556)
                     /locus_tag="P5541_01060"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRV70593.1"
                     /translation="MRRESGLPVMVAISGQGKNGTKDLTGRISLSEEAGNGCRHVVVE
                     MEETIPETKTGQFFMVRVTGVKDPLLGRPLSFTFASGKKVGFLFRVVGRGTSLLASLP
                     PGARLDLRGPCGNGFPDPSGKQLVLVAGTLGIAPFLDLVDISPGQPEKTFVLGLPGKG
                     WELFADWCSSRIPGLVISSDDGSFGIRGTAIDAASSLLSPDCEIWGCGPNGMLRSLGQ
                     INPMGKTLVSLESRMACGIGGCMGCSVETRRGMVRTCVEGPVFDWKEVVWDGQ"
     gene            complement(15529..16821)
                     /locus_tag="P5541_01065"
     CDS             complement(15529..16821)
                     /locus_tag="P5541_01065"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRV70594.1"
                     /translation="MSILLKNAKLFDGRTFSAEITDLFVENGRVSAIGGGLDGSGAKE
                     VIDLSGKILSPGFIDLHCHLRDPGQEWREDLHSGSLAGAAGGFTTLVCMPNTDPPVDN
                     AALVRYLVDKGEGCKGARVLPAGCVTRERRGTTLAEMGKMALAGAVLFTDDGSPVSDG
                     KILRLALQYTSRFGVRIMEHPEDMTLTMGAQVNDGTCSSLSGLKGWPAAAEAADIYRG
                     ISLSADTGVPLHFTHVSAAESINAVRAGKSSGCKVTCDVTPHHLCLEETMVIDGGYDP
                     VFKVNPPLRSGSDRDALWEGIKDGTVDAIATDHAPYHTDEKDVPFQEAAFGIPSLECA
                     AAAVMDEWEKRGKPCSLEKILSLFTSGPAAILPERWKTLGSLTPGAPADLTVIDPGLE
                     RLVDASTWFSKSRLTPWDGKKLKGWPVMTICGGKVVFR"
     gene            complement(16818..17747)
                     /locus_tag="P5541_01070"
     CDS             complement(16818..17747)
                     /locus_tag="P5541_01070"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRV70595.1"
                     /translation="MNWKHRHVLDLDKWERDDIQHIMDQTRSMDELLDRPIKKVPALR
                     GKLVVNLFFEASTRTRVSFELAEKFLSADVVNWSASGSSTSKGETLRDTAWTLESMGA
                     DAVVIRHGAVGSADYLARKLKKSVVFNGGDGGHAHPTQALLDVYTAWKKFGALDGAKV
                     AIIGDVLHSRVARSGIIAFSKMGARVSLSGPATLMPKSVDTLGAVYEEDPVMAVNDAD
                     IIYLLRIQRERQDEGLFPSLDEYHQLFGADKSLLEHARPDAMVMHPGPINRGVEISSE
                     VADGKQSMILGQVRSGVAVRMALLYVCLGGAKQ"
     gene            complement(17744..18298)
                     /gene="pyrR"
                     /locus_tag="P5541_01075"
     CDS             complement(17744..18298)
                     /gene="pyrR"
                     /locus_tag="P5541_01075"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRV70596.1"
                     /translation="MLTEKATVMTAQDIERVLKRMSHEILEKNRGLENMIIVGIQRRG
                     VNLANRLRDILAHFESTRIPSGVLDITLYRDDLTLLNDQPVVHSTSIPEDVTGKKILL
                     VDDVLYTGRTIRAALDALMDLGRPSAVQLAVLVDRGHRELPIHPDYVGRTVPTSRSEV
                     IEVRVMELDNKDEVVICEREEEKE"
     gene            complement(18448..19161)
                     /gene="rnc"
                     /locus_tag="P5541_01080"
     CDS             complement(18448..19161)
                     /gene="rnc"
                     /locus_tag="P5541_01080"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRV70597.1"
                     /translation="MTRNEGPGSIPGIEKLENFIDYRFENRNLLVEALTHASFSNENG
                     MCPFNERLEFLGDAVLGLCVTAMIYKKFPDEDEGAMSGMKASIVCEDALYAWSVRAGI
                     PGLLRLGRGLSQSGGSRQPVICADAAEAVFGAVFLDGGYGAAEKVIGSYYVSSIEANV
                     GVPRNAKAVLQEMMEQNGLGKPEYIVAEKSGPPHAPVFRVNVFCSGGLIGEGEGRTIR
                     EAERKAAEKALSCNRLHIF"
     gene            complement(19177..20418)
                     /gene="fabF"
                     /locus_tag="P5541_01085"
     CDS             complement(19177..20418)
                     /gene="fabF"
                     /locus_tag="P5541_01085"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRV70598.1"
                     /translation="MRRVVITGMGVVSPIANGREDYWRFLEEGRNGVGPITLFDASDY
                     TVKIAAEIRDFDPGEWMDRKDARRADRVIHFSVAASEMAVRDASLDAGNLDPDRFGVY
                     IGSGEGGIATSYEGMKTLLEKGPSRVGPFCVPMMLSNMPAAYVAIVYGAKGPNICVVT
                     ACASATHTMGEAYHTIVRDDADIILAGGAEAAITPVAVAGFAAMRALSTRNEDPSHAS
                     RPFDAERDGFVMGEGAGVLVFEELEHALKRGANIYAEIKGFGSTCDAYHITAPDPDGS
                     GPAKAMLQAVRKAGWKPEDVDLINAHGTSTSLNDRMEAAAIRNAFGDLADSILINSTK
                     SMIGHTLGAAGALETVAAIQSICEGVVHPTANYVTPDPRCPIKVVPDGKALKKNVERL
                     LVNNFGFGGHNGVIAIEKFRK"
     gene            complement(20530..20781)
                     /gene="acpP"
                     /locus_tag="P5541_01090"
     CDS             complement(20530..20781)
                     /gene="acpP"
                     /locus_tag="P5541_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRV70599.1"
                     /translation="MKMEEITARLREIVMDRLDVEEDQIKPDASFVEDLGADSLDIVE
                     LIMGIEEEFDIEIPDEDAEKLTNVGEAMEYIKGKLGVEE"
     gene            complement(20821..21567)
                     /gene="fabG"
                     /locus_tag="P5541_01095"
     CDS             complement(20821..21567)
                     /gene="fabG"
                     /locus_tag="P5541_01095"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRV70600.1"
                     /translation="MNPESRVALVTGAGRGIGRSIALKLASKGHRIAINYRTSSAGSL
                     EVLEAIRSAGGEGMAFQADVSAEKDVAELFRQIEEKLGTVGILVNNAGITRDGLLMRM
                     KDSDWSDVIRTNLESVFLTTRHASRNMAKSRWGRIINISSVIGLIGNAGQANYAAAKA
                     GILGFTKSTARELGSRGVTANAIAPGFIETDMTSILDQKVKEQMMLQIPAGRSGSPED
                     VASLVAFLASEEASYINGQVIAVDGGMTMV"
     gene            complement(21569..22516)
                     /gene="fabD"
                     /locus_tag="P5541_01100"
     CDS             complement(21569..22516)
                     /gene="fabD"
                     /locus_tag="P5541_01100"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRV70601.1"
                     /translation="MKYSLVFPGQGAQEVGMGKDLVDCYSSARDVFERCDGALGFGLS
                     RLIFEGPDEELKKTEYAQPAILAMSIAVLRSLQKEEGLDLDPLYVAGHSLGEYTALVA
                     AGALSLEDGIRLVNLRGKLMQEAVPLGKGSMAAVIGLEREKVLAVCEEASVMGVCQAA
                     NFNAPGQIVISGETGAVDMAVVLCKERGAKRALPLKVSAPFHCSLMRPVADKLREAFG
                     NCSWKAPKWPLVANVSAEEENDVESVQEALFLQTFMPVLWSDSVEKMITAGTGAFLEA
                     GPGSVLSGLIKRISPWAECFSANTCENIPGAIDFLREGQ"
     gene            complement(22536..23486)
                     /gene="fabK"
                     /locus_tag="P5541_01105"
     CDS             complement(22536..23486)
                     /gene="fabK"
                     /locus_tag="P5541_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HRV70602.1"
                     /translation="MLSDNRIKRILGTEYPVIQGGMAWVADADLAAAVSNGGGLGIIA
                     AGSMPPDILEQQIIKVRSLTEKPFGVNIMLLSPTAEDALEVVAKNRVPLVTTGAGSPG
                     KVIERLKPLGIKVIPVIASAAHAKRVEKQGADAVVAEGMEAGGHIGEVTTMLLVPQVV
                     DSVSIPVVAAGGVADSRGVIAVFALGAEGVQVGTRFVCAEESNAHTKYKEAIIRARDR
                     STVVTGMSTGHPVRCIQNRLTREFEKIEKEGASVEEIEILGTGKLRAAVVDGDVDYGS
                     VMAGQSAGLVNRIQPARDIILDLFGGISEVLPVLQKNLFN"
     gene            complement(23496..24491)
                     /locus_tag="P5541_01110"
     CDS             complement(23496..24491)
                     /locus_tag="P5541_01110"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRV70603.1"
                     /translation="MALDFAVPVALLGTGMYVPEKIVTNEDLSKLVDTTDEWITERTG
                     IKERHIASEGEVTSDFAARAGMEALKAAGVSPEEIDMIIVGTNSPDSLFPGVGPLVQG
                     KIGAVRAGACDVQAGCTGCVYALAMASAGIGSGLWKKVLVIGAEALSRIVDWNDRNTC
                     VLFGDGAGAVVLGATGGKNSVLAVELHADGALGDYIEFPGGLAAKPATCATVEAGEHF
                     VRMKGNEVFKYVNRIIPPFVEDLCEQAGLDVSEIDSWIFHQANLRIIEGVLRRLHVPV
                     GKAVVNLQKYGNTSAASVFLALHEGISGGKINRGDKTMLVSFGAGMTYGAIILEI"
     gene            complement(24481..25494)
                     /gene="plsX"
                     /locus_tag="P5541_01115"
     CDS             complement(24481..25494)
                     /gene="plsX"
                     /locus_tag="P5541_01115"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRV70604.1"
                     /translation="MLLALDAMGGDHAPLETCAGAIMACEKFPELEIALVGKSSSIEP
                     LLESAGGDIRGRLHIVHTDEMITMDEPPAVAIRKKRHSSLRVAMQMVRSGEAGGCVSA
                     GSTGAIVAGGVLVVGRIQGIDRPGLGVPLPAVDRLSMLIDIGATVRCKPLNLYQFALM
                     GDVYMRKIAGVQNPSIGLLSNGQEEVKGDELILEARDLFRKSGLNFIGNVEGGGIPFG
                     VADVVVCEGLTGNILLKFIEGTGEAIYTLVKDEFNRRFMPKIGMAFMLPMLKDLWSRF
                     NYEETGGTPLLGVNGAVIKAHGRSRASAICGALRVARDFVSRNGVQAIAEELTEGGKN
                     IGA"
     gene            complement(25478..26092)
                     /gene="fapR"
                     /locus_tag="P5541_01120"
     CDS             complement(25478..26092)
                     /gene="fapR"
                     /locus_tag="P5541_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HRV70605.1"
                     /translation="MKRSAGKKERHGRIVKNLEANPLLTDEEIAGKLGVSVSTIRLDR
                     ALLGIPEVRERMRSMASQAGSRLRSLKRDEIIGELLELQPDEWALSTIQTTGDMAFRH
                     TGFVSDHYIYSMASCLAVAVIEADMAVTGSARVRYRHPAKVGERLVARAKVGTKKGNK
                     YVVSVRIKVGEREIFLGRFITVVGECDKASFENKEESDTGASGS"
     gene            complement(26410..26622)
                     /gene="rpmF"
                     /locus_tag="P5541_01125"
     CDS             complement(26410..26622)
                     /gene="rpmF"
                     /locus_tag="P5541_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRV70606.1"
                     /translation="MATPKSRTSHKRTSQRKSHWLGSLSAPGTTLCPQCGEVAQSHRV
                     CPECGYYRGRQIIVKKTKGEEEKKES"
     gene            complement(26591..27244)
                     /locus_tag="P5541_01130"
     CDS             complement(26591..27244)
                     /locus_tag="P5541_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRV70607.1"
                     /translation="MDEGSKGNRIFLEKPANWICGFDGSEALRTDAPVDVNCSLPVGG
                     DIEYWGQRYSIPGVITLEGKVYGDRSCLVADMALTVAVSVPCARCLEETPLDISRKFR
                     YFYKPLPGSEPKEGEAGEYTVLVETLDTCIDITDQIWESLIVSLPEKVVCSEDCSGLC
                     PFCGANRNREDCGCSEGPVDPRLEKLAGLRDAINETGENIPGKGGNQIGHTKKQDVS"
     gene            complement(27257..28453)
                     /locus_tag="P5541_01135"
     CDS             complement(27257..28453)
                     /locus_tag="P5541_01135"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRV70608.1"
                     /translation="MKVLVLNCGSSSLKYQLFDMKNEGVLAKGLVERIGIDGSRIKHE
                     KTGQDPHKADVPIPNHKVGIKIVLEALLDPDHGVLKSLDELKAVGHRVVHGGEKFARS
                     VVITQEVLDVIEECVPLAPLHNPANLMGIRAMKEVLPNVPQVAVFDTAFHQTMPDYAY
                     TYGLPYHYYEKQRVRRYGFHGTSHFYVAFRAAEMLGKPVEELKIVTCHLGNGSSISAV
                     KGGRSVDTSLGFGTVPGVLMGTRCGEIDPTVVLYLMEQEGIDPKSMSHILHKESGIQG
                     VSGISSDLRDVEEAASQKNPRAMLALNILCYGIRKHIGSYAAAMGGIDAIAFTAGIGE
                     NSVTVREMVCNGLEFLGAKIDLEKNRVRGKEAEISQEGSAVKVLVVPTNEELVIARDT
                     LELAIG"
     gene            28543..29796
                     /locus_tag="P5541_01140"
     CDS             28543..29796
                     /locus_tag="P5541_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV70609.1"
                     /translation="MAKKRIVGIIAEYNPFHNGHLYHMKRAMETAEAQATVIVLSSSF
                     VQRGEPAFTDKWSRTEMALRGGASLVIELPVAFSCHNAGVFGSAAVDLLFSTGVVTDL
                     SFGMENPDAPLEQIAKVLTSEPPKFRRSLKTLLAEGFSYAEARSAAADVVIPGARAVL
                     SSPNNTLAQEYVSRIFARGYDLRALPVKRLGEGYHSDILDPFASASRVRKAIRSQGKG
                     DPATEALPEWSQRILDREISRGRCLLSLDLYWRILSTVLIREDPETMAGHAEMKEGIE
                     NLLAAKARRCLSFEDFAGACTSKRYPKSRIQRHAAHVLLNYGHSLNRRAQREGPPYLR
                     VLGFDDRGAEILRSMRETSLLPVVFHPRGRPGTYQREIADLEYRASAIWENILSSPDP
                     GRESRTPPVRPDLDPCRRGTSPEVS"
     gene            complement(29744..30244)
                     /gene="coaD"
                     /locus_tag="P5541_01145"
     CDS             complement(29744..30244)
                     /gene="coaD"
                     /locus_tag="P5541_01145"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRV70610.1"
                     /translation="MIRAMYPGSFDPVTNGHIYIAERAAAIFQDLMVGVLNNRQKKYT
                     FSKEERETMTREALSHLPNVRVKQFEGLLVDFMREEQCRVIIRGLRALSDFEYEFQLA
                     LMNRQLAPEIETFFIVTEARYSYLSSRAVKEVFHFGGSINDMVPPGVFRRLRERFPSG
                     KDPGPA"
     gene            complement(30251..30748)
                     /locus_tag="P5541_01150"
     CDS             complement(30251..30748)
                     /locus_tag="P5541_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRV70611.1"
                     /translation="MKEARPTSGLVAQALFNILGPAEERSFLDLFAGSGKIGLEAFKR
                     GFSPVYFVELSRKNADEIETRAGGGEKRVLRMDVRRSFAYFGNKGLVFDVVFADPPYC
                     SGWAARMGSLKSRLSGIIKGGGFLVLEHSKREIPDPASWKEWIVSSRQYGETVLSIFQ
                     KKLKG"
     gene            complement(30745..31182)
                     /locus_tag="P5541_01155"
     CDS             complement(30745..31182)
                     /locus_tag="P5541_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRV70612.1"
                     /translation="MNGYPMTPIGFVESPIKKPVPPDEFRGVKSRIRVLHRFLPALAG
                     LEEELRLAIIFRFHLSWGYSMKVHPRGDLNRPIRGVFSTCSPRRPNGTGLSFVRLLGV
                     EGLVLAVIGLDAVDGTPVLDIKPLRYLEDPAGPDETKERGNIS"
     gene            complement(31179..32114)
                     /locus_tag="P5541_01160"
     CDS             complement(31179..32114)
                     /locus_tag="P5541_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRV70613.1"
                     /translation="MVPLGNIIINEDPRKKICQIANVDPPFRALEIGFGNGEFLEYLA
                     SSSPRGVVFGIEVSLQCTARAVKRIQRSCLKNTRIVLGDARFVLGECFPPEWLHAVYM
                     NFPCPWPKKRHAKRRVTYRGFSDVLAGSLAMGGAFEIFTDDPEYADEVAGELPLHPAL
                     SLESSILNPDREIRTKYERKWREQGKDLFLLRFRKKEPFLLPARKKEEKELHVSLDTP
                     CPDLSIMSRLTGVEGKSASSRWVFKESFIGPSGVILVEAITSDEGFRQTFFIRIVPRG
                     EGCLVKVDSFSRPFKTEAVSLAFSSLVEFLAGDKS"
     gene            32427..33002
                     /locus_tag="P5541_01165"
     CDS             32427..33002
                     /locus_tag="P5541_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV70614.1"
                     /translation="MSEVIEVRWHGRGGQGAKTGSAILAEVLFEAGKFTQSFPEYGAE
                     RQGAPMKAFNRVSEKPIRVRSGVLQPDIVVLIDPTLIDAVRPTDGGKDDTVYLINSPD
                     DPKVIRKKLGIPASAKLMTVDATKITLEEFGQNRPNTPMLGALSHCFPDVPVQAFIDH
                     FTHKMGAKLPEKVLTANQNAIRRGHEEVHQG"
     gene            32999..33295
                     /locus_tag="P5541_01170"
     CDS             32999..33295
                     /locus_tag="P5541_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV70615.1"
                     /translation="MSSLKGWKDLPPATTVPASTSEAVQTGQWRSMRPVWDEEKCIHC
                     LQCWIQCPDKSIKANADGKVTGIDYFYCKGCGICAAVCPKNAIEMRPEAEFIEQ"
     gene            33324..34490
                     /gene="porA"
                     /locus_tag="P5541_01175"
     CDS             33324..34490
                     /gene="porA"
                     /locus_tag="P5541_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRV70616.1"
                     /translation="MPRKDITSGNWAVAEAMRQINPDVVAAYPITPSTDIPMRFSEFV
                     ADGLVDTNLVTVESEHSAISACLAASVSGARVMTASSANGVALMHEIFPIAASYRTPI
                     VFGLVNRCLGAPINIHCDHGDSMAERDSGWIHIYCEDGQEVYDTMIMAVRIAEHKDVL
                     LPAFVCQDGFITSHCFEPVEFLDDEQVTKFIGEREPLLSLLDVDNPVAYGSLALTDYF
                     FEIKRQQVEAMKHVEKVYNDVAADFAKITGRNYPLVDKYKTEDADYIMVIMSSAAGAA
                     KETVDKLREQGHKVGCLRIRLFRPFPASEIVGALKGAKGIAVCDRAASLGAAAPLFTE
                     IKGALYDAGIHIPANTYVYGLGGRDFFPHNAEEAFMDLINNKMSPEERYLGLRE"
     gene            34504..35448
                     /locus_tag="P5541_01180"
     CDS             34504..35448
                     /locus_tag="P5541_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV70617.1"
                     /translation="MAVKLKDLITKERAMRQGHRMCPGCGAPTVVQQALLAVDDPIVI
                     TSATGCLEVSTTIYPYNSWGVPFLHSAFENAGANVSGIEAAYVALKKKGKIKKNIKFV
                     AFGGDGGTYDIGLQALSGAAERGHDFTYICYNNQGYMNTGAQRSSATPIGANTSTAPA
                     GKVIPGKIQKPKDLTAIMAAHHIPYVAQTTIFNPMEVIEKVKRAIETPGPAFVNILAP
                     CVLFWRLEPSMQKEICRLAAETRFWPIYEVINGEWKLSYKPKKYVPAEEFLKPQGRFR
                     HLLKGETGKAVIENFQKEIDEEWQYVLKRCGETWEPQA"
     gene            35525..35602
                     /locus_tag="P5541_01185"
     tRNA            35525..35602
                     /locus_tag="P5541_01185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35560..35562,aa:Pro,seq:ggg)
     gene            35675..36541
                     /gene="rsmA"
                     /locus_tag="P5541_01190"
     CDS             35675..36541
                     /gene="rsmA"
                     /locus_tag="P5541_01190"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRV70618.1"
                     /translation="MTERFHRANTRIGQNFLVDKNILKIILDHAGISSEDTILEVGAG
                     KGILTRGILEGNPRMLYSLEIDRSLEPFLKELSDSFSNFRIIWGDALREGFPPSFDPT
                     PNKMVANIPYHITTPLIWKVLESLAEKGLKYLLLMVQKEAAERICAPARCKERYPLGV
                     TIQSMGEARILRLVSPESFRPTPSVTSALLEINLLTREDLPNCQLWRRLLRSGFAQRR
                     KTLVNNLGRDFPELKTKIASIINSLGFPVTVRAEELEVEEWHDLLRIFRTVLATKKPE
                     TSVTHPARNRQP"
     gene            complement(36554..36829)
                     /locus_tag="P5541_01195"
     CDS             complement(36554..36829)
                     /locus_tag="P5541_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRV70619.1"
                     /translation="MTKADIINAVAKSAGISKKQAGEAVASTFETIVKSLSKGEKVQL
                     VGFGTFEVRSRAPRKGRNPQDPSKVINIPGKKVPAFRAGKALKDRVR"
     gene            complement(36957..38276)
                     /gene="der"
                     /locus_tag="P5541_01200"
     CDS             complement(36957..38276)
                     /gene="der"
                     /locus_tag="P5541_01200"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRV70620.1"
                     /translation="MAIVAIVGRPNVGKSSLFNRIVGRRASIVDDQPGVTRDRIYGEV
                     EQDGRRFLLVDTGGLFPDGGDDITENIRRQVRLAVDESDVLIMVIDTRDGVTPLDREI
                     ALFLRKSGKHVVLAANKVDDRVHESRVMEAHALGFGDVFGISAAHGRNTDELVEKVIS
                     LLPPGDPDSLRNPDEIAIAIVGRPNVGKSTLLNLLSGTERSMVSPVPGTTRDSIDSRV
                     LIEGRPLRLIDTAGLRKKSRISSDVEYYSLVRTHESIERCDVAVLVMEGTEPCTEQDK
                     RMAGLVLEKGKGLILAVNKWDLLTKTPELGDKVTEKLRDEMPFVGFAPVVFISGLTGR
                     GVHKLPETIFRVHANRSRMMDPARLNTMLRDTLSFERLPVGPRGMPLRMKSCSQVAAR
                     PPAFVFIVNDPAIVTKAFERHVAKKLRELDDFEGTPLRLFWRSKSKT"
     gene            complement(38295..40094)
                     /locus_tag="P5541_01205"
     CDS             complement(38295..40094)
                     /locus_tag="P5541_01205"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70621.1"
                     /translation="MFLEVVVPGPWWHTLTYEFDRVCSRGARLRVPVGRGVRVAFATG
                     SSSMERPFGEYRVLSALEAIDDRSPLGWELFNTSEKIGKHFLCGFGEALKAVAPASVI
                     SGKSFEGLPEMDGPRGDFNEERCDSPDFSARSDRYLEVIESSRGGVLALFPDRSAASA
                     FWKKLPEKHKKDSLLWLSAPGPASDSRWERVRRGNFRLVVGSPAALFAPLTSVSAVIV
                     EDEGNPSYLSRRFPFIHARSAAGSRAQSWGATLVTGGGVPSSRSFFRMPRECPREATG
                     RMVFVDMGMARKISVRGIQSPLLVSDSALKRTQAEVSGGRPVLWILDRKGYAGAVICS
                     ECGRMIVCPSCGLPVRWDDKEEALVCGFCGKKTALAEVCPFCGAISLQGMKPGLESLR
                     HIGENVLGDSCPVYIWHADIRQTVSARKDLVKGLASGGLVVGSRKAIELCDHLEIGLV
                     CWLDADMAVNTPFYDARSRAFRMVWESAWRGKGNSFRTVLIQSRVPGSGWQKGLAAGW
                     DHFWRAELMERKELGLPPWRYLVEIRGLKGMKAETRDLLATSGIECLDPDPLEDMLWA
                     RSGNLSRVRKALAPLFHISRSGRGFPRIEVWAD"
     gene            complement(40097..41323)
                     /gene="coaBC"
                     /locus_tag="P5541_01210"
     CDS             complement(40097..41323)
                     /gene="coaBC"
                     /locus_tag="P5541_01210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRV70622.1"
                     /translation="MPMDWKKNRKLLLGVTGGIAAYKAPETVREFRKHGWEVEVILSR
                     SAERFVSPLSISSLSSRRVWLEDDFLSAERGWKIPHISLAEWADVMIVAPATASFLHG
                     AAFGDGSTLLHASLLAARCPVLIFPAMNVNMWEHSAVRDNAKKCSELGYHLVVPESGE
                     LACGSEGKGRLPSIDVIREETFRAVCPSADLDGVSVLVTSGPTREAIDPVRFISNPSS
                     GKMGSAVARTAWYRGAEVVMISGPSSQPVPYGIRVIPVVSAIEMYDAVMLEAPAYDVI
                     VKAAAVGDFTPSRVMDRKIKREKEKNLTIEFTPNPDIAASLGASKRPGQYLVGFAAET
                     EDAVANASAKLVRKGLDLIVANSVSGDASAFGSEYNSVVMIGKEGIEKEISGTKEEVA
                     DAVWGIVLKKLEAAGL"
     gene            complement(41295..41561)
                     /locus_tag="P5541_01215"
     CDS             complement(41295..41561)
                     /locus_tag="P5541_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRV70623.1"
                     /translation="MTKKTDGMDLSRPITNKYLMACAISKRARQLSEKKGRMPFGDEG
                     SNPIEMAMREISEGKVSIFGMESASPAVAEKEAADNADGLEEKS"
     gene            complement(41591..42157)
                     /gene="gmk"
                     /locus_tag="P5541_01220"
     CDS             complement(41591..42157)
                     /gene="gmk"
                     /locus_tag="P5541_01220"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRV70624.1"
                     /translation="MARGRLFVVSGPSGVGKGTLRKALFNTLDNLEYSISCTTRSPRK
                     GEKDGLDYRFISPGSFRRLAEEGMFLEWAEVHGNLYGTLKEDVEASLSKGRDVVLEID
                     VQGAVKVKEAMPDSVLLFILPPSEKDLLERLSHRGTESVDEVQARIEGARHEIELSGM
                     YDHVIVNDFFSRALGELAGIVESYRRLR"
     gene            complement(42150..42416)
                     /locus_tag="P5541_01225"
     CDS             complement(42150..42416)
                     /locus_tag="P5541_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRV70625.1"
                     /translation="MGYNLLHIGFGNMVVSERVVAIIHPSSAPIKRLKDEARAQGKLV
                     DATEGRKTRAILVMDSDHVVLSAIQPETIGVRFEGRDRECETDR"
     gene            complement(42421..43311)
                     /locus_tag="P5541_01230"
     CDS             complement(42421..43311)
                     /locus_tag="P5541_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRV70626.1"
                     /translation="MKMLRSMTGFSRTSIDRPFGSISLEISSVNHRNQEIFVKSPREL
                     FRFEQTIQQEIRKASRRGKIQVRLEVCWGPAFRGAVIDRIVLDGYIRDTAKARAAFGL
                     PEDVQVEQLLSLPGVMVSSQETEELEKEIEEAILSLVAAGIREWVDMKKTEGSHLEKE
                     ILAHLEGLESRVIEIENRWKAAKAGALSSMKERIISFVESGGFDCDEGRLAQEIVILN
                     DKWDISEELARLASHMDKFRSMVDNKGVNGKTLDFLVQEMNREINTVASKVQDAEIRW
                     LSVEAKSELESVREQIQNVE"
     gene            complement(43265..44434)
                     /gene="hflX"
                     /locus_tag="P5541_01235"
     CDS             complement(43265..44434)
                     /gene="hflX"
                     /locus_tag="P5541_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRV70627.1"
                     /translation="MTDRGKGWPSTAILVAISGKDPEGLDASLKELSLLLENLGTGTA
                     GQMIQRRDKPDPDSFIGSGKAQEIALLARESGASLLVVDGRLSPAQAAHLSEITGLMI
                     WDRPLVIMKIFEQRAQTGEAKLQIELARCRYEIPLLKGLGMQMSRLGGGLGTRGPGET
                     EFERHRRKLERREREISRKIEIIRERRQAQRKRRVRAGLRTVALAGYTNSGKSSLLKC
                     LSGDKSLVSEDRLFSTLDPFVRRVRLPGGSFILMTDTVGFIRNLPHDLVTAFRATMEE
                     VSGSDLVLLVADASFGGFNDDISVVETLLQSIGAEEIPRMVVMNKIDLLEPEEASSRI
                     AEVGRTQDIVIPVSATRGDNIGTLLENIEKFFGYVTGKGLDEDAQKHDWFQQDQY"
     gene            complement(44424..45062)
                     /locus_tag="P5541_01240"
     CDS             complement(44424..45062)
                     /locus_tag="P5541_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV70628.1"
                     /translation="MKSVFYWLVAGFFRIMLLVYNRLQVTGLERALSVKPPIIIAANH
                     CSNLDPIVIGAAFPRRLRYLAKSELFSFPPFGFAIRSLGAVPAKRDDLQGAAGALKFL
                     LERINKGESVLLFPEGQRSPDGSLQPLEGGAGLLAAKTGVPVVPAYVHGTFEALPRES
                     SRYRPVRIRLVFGDPIYPAGIKGVPVKELRQKITEKLENSLRKLEGELSVDR"
     gene            complement(45064..45768)
                     /gene="cmk"
                     /locus_tag="P5541_01245"
     CDS             complement(45064..45768)
                     /gene="cmk"
                     /locus_tag="P5541_01245"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRV70629.1"
                     /translation="MISSDHADSTVITIDGPAGAGKSTIARLLAGRIGFQYLDTGAIY
                     RTIAYCLSRNSVPPVENDFLCSSLACCRIGLEKGKIILDGKDVSDQIRSQKIDRIVSS
                     YAALPMIRRFLIGIQREQAAMSDIVADGRDMGSVVFPEAALKIYLDADVSVRAERRML
                     ELSARGETPEIGEILENIRERDRKDREREVSPLMVPEGAHIIDTTGLDVDEVVTRIMA
                     LVSPVIGGSGRPERSG"
     gene            complement(45791..46540)
                     /locus_tag="P5541_01250"
     CDS             complement(45791..46540)
                     /locus_tag="P5541_01250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRV70630.1"
                     /translation="MGSMNDVDKGFFRLNRYLAMSGAGSRRNVENLVRSGRVSINGMA
                     VRDLSTRIRPGDNVCLDGLVVVPKPEVYLVMNKPVGIVCAVKDKYYPTVIDITPADAV
                     EAGVFPVGRLDKDSEGLLILTNDGAFAQKIIHPEQGIGKTYEVLLEKEADQAGIEKLR
                     MGTTIEGKRLIPLNVELVAGEPERRWLSITLSEGVKREVRVMAKAAGFRVMRLIRRKI
                     GHLELRKIRSGAIVRISGDELERMILKGGTV"
     gene            complement(46524..47114)
                     /gene="scpB"
                     /locus_tag="P5541_01255"
     CDS             complement(46524..47114)
                     /gene="scpB"
                     /locus_tag="P5541_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRV70631.1"
                     /translation="MSEYTAERPLSPVARSVEAVLFVATQPVCVRELASVAGVTDGQV
                     VRALDEISGRYRSGHGLCLLNIAGGWMMATSPEQEEVVALFRDVALTQKVRLSKAALE
                     SLAVIAYAQPVTRSEIEEIRGVRCERVLETLLKHGLIRVAGRKKGSGTPLLYRTTDSF
                     LSIFGLGAISDLPTLEEMQEMVLPEDEAGNSNGIDE"
     gene            complement(47095..47832)
                     /locus_tag="P5541_01260"
     CDS             complement(47095..47832)
                     /locus_tag="P5541_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRV70632.1"
                     /translation="MIFEVELEGFSGPLDLLCNLVESGEVQASRISVSELIRVYYDFL
                     LKNERAGINEMAAFFSLSARLLLGKLKSLLPALPGEEKPEIEDVPEGEDLFDALGRYM
                     PYRRASAHMIELMRARARCFTRNSHEEGPPWFDLGDLYSLSMVWWEIVSEKKERSEGQ
                     GRELENIEWDGIPVATPEEGQVEVRMDEIRELVSGKGFVFLSDILVRSSTRPLLVVTI
                     LALLEMARLGIIRISQEEMFGDVGIHC"
     gene            complement(47866..48858)
                     /gene="trpS"
                     /locus_tag="P5541_01265"
     CDS             complement(47866..48858)
                     /gene="trpS"
                     /locus_tag="P5541_01265"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRV70633.1"
                     /translation="MKKRVFSGMRPTGRLHLGHLAGALSNWVRLQEEYECYYCIVDWH
                     AIMSEYADTGSVRANCREVLLDWLGVGLDPERSTIFLQSHVPQHAELHLALSMIAPLG
                     WLERNPTYKEQIINMQNKDLSTYAFLGYPVLQAADILLYKGDLVPVGEDQSAHLELSR
                     EIARRFNHFYGDIFPEPRTLLTETPKVPGTDGRKMSKSYGNSINIADAPEDIWEKLRT
                     MMTDPARERRKDPGDPAKCPVWDIQKVFNPDAGEKERLADGCRSAGIGCLDCKKALYG
                     HLMDVLGPIQDRRRSFELKEDGVLEVLRSGAGRARDVASATMSQVIDAIGFVDR"
     gene            complement(48855..49667)
                     /locus_tag="P5541_01270"
     CDS             complement(48855..49667)
                     /locus_tag="P5541_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRV70634.1"
                     /translation="MTRTKDVIRAIEEFAPFAWSCEWDNPGLQAGSPEWEVKGVAVSL
                     DATEQALSEAIKQKCSCLVVHHPMVFDPLRSIQTQTVPGGKILKALDHRISVIAAHTN
                     WDVSPCGVNALLGEKLGLAGPVPLEDGPEGSWGIGLCGDLKAGIPEKDLGDFLCDSWK
                     LSWVRSYNLPKSPSRIALVGGSGGEMWPLALSKGADVFITADMKYHQLVDAVESGLGV
                     VVVDHGEMERLSIPVLRKMLEGFLPVKVIEVDKRGFDPGEITKSTGQRGERV"
     gene            complement(49727..49803)
                     /locus_tag="P5541_01275"
     tRNA            complement(49727..49803)
                     /locus_tag="P5541_01275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49767..49769),aa:Ile,seq:cat)
     gene            complement(49850..50032)
                     /locus_tag="P5541_01280"
     CDS             complement(49850..50032)
                     /locus_tag="P5541_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRV70635.1"
                     /translation="MIVKVNRDECIGCGVCAQICPEVFELDENAGKAKVINQSGAECA
                     REASDSCPVGCIAVEE"
     gene            complement(50242..51039)
                     /locus_tag="P5541_01285"
     CDS             complement(50242..51039)
                     /locus_tag="P5541_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRV70636.1"
                     /translation="MKSTRTERLIRMASRFLVAPSRQISLTGLSEQFEVSKTVISDDV
                     AIIDRSMKEEGIGGICVERGRSGGAFLRPKLSREYRKSFLGSIAETLSQDNRILPGML
                     IHYSDILFNPGFAGPLGYALASDFADREPDVVMTSEVKGIPLAVFTAHALGIPMAVCR
                     FRNRPSDGPAVAVHYPSGSGEVRTMFMGTRFLSAGKKVLIIDDFMRGGSTVAGMLLVA
                     REFKAEVVGTGVFIAASEPARKAVSEYSALLRLERGSDGIPEVKVQA"
     gene            complement(51045..51833)
                     /locus_tag="P5541_01290"
     CDS             complement(51045..51833)
                     /locus_tag="P5541_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70637.1"
                     /translation="MGTLFLRLPAAEKLTLSINDHNNVSEHILRVHGFSIRGKNILDK
                     VLKIARRKSSALPFFEIDLWKKIPPGSGIGSGSGNAAALARFLTDEGFVQFSEEEILS
                     LGADVPFLFGGERAGYRSGIGDIPSPLRGVEKISPQVLVVVPCWSQNTAEAYRLADDL
                     YRSSGWPMSDKDAREEALRIARILARKEKTGLLPNDFFPVISARHPEYHDFARIADSS
                     GSFAWGLGGSGSSFFCLFREGAGLMKAVELFKRHDVARHIFDLG"
     gene            complement(51981..52232)
                     /locus_tag="P5541_01295"
     CDS             complement(51981..52232)
                     /locus_tag="P5541_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRV70638.1"
                     /translation="MSPNLSTIRDRVNEYKGSRVRYKALKGRRKTEVRMGVIMETYPN
                     LFTMYVESQRSTVSFSYAELLTREVEVEILPGNKKLIQF"
     gene            complement(52422..54350)
                     /gene="selB"
                     /locus_tag="P5541_01300"
     CDS             complement(52422..54350)
                     /gene="selB"
                     /locus_tag="P5541_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRV70639.1"
                     /translation="MMTQNREVPVVLGTAGHIDHGKTTLVKALTGVDCDRLRDEKRRG
                     ITIELGFAPVTLPGGQVVSIVDVPGHEKFIRQMVAGAAGIDGVLFIIAADEGVRAQTR
                     EHLDILGLIGVRHGIVVVTKADMVDPDFLDLALEEIREEIEGTFLKGAEIIPVSAVSG
                     FNLDKLMESIGIMVGKISPRNSGGGFFMPVDRVFPVKGFGSVVTGTAYRGSVRKDDPL
                     ELLPLGLKTEIRSVQVHDVPVETGTGGQRIAMSLGGISLDEISRGDVLCTPGVFSATE
                     RFDVRLSILPSSPEAVEHWQRVRMHLGTADLLGRVSFFDREKLEPGDTAFAQIVAEEP
                     VVCTRDEAFVIRFYSPLRTIGGGTVVMPLSRRPRGHKEKISHLAFLKKISTAADTEER
                     FKELSSHMKLLPVREAISLLQEVPSEFARIAKEADRKGRSIYIETGEGLVISGEYARE
                     KLAETKVLLDGMHEKEPENPGMVADVLCRKLFPEIDIKYSKRILDRWVENGEVALDDQ
                     RVSMPGFTPRDASQLGASGRTILEYCSRREFQPPTLDEISEGTALSAAEIARSLEALR
                     AQGQITLAGGEFLVSRNVLEKLVEKLAGAGGDITVASVRDLTGSSRKYVLPMLEMLDS
                     MGITRRVQDKRILRKTKV"
     gene            complement(54331..55746)
                     /gene="selA"
                     /locus_tag="P5541_01305"
     CDS             complement(54331..55746)
                     /gene="selA"
                     /locus_tag="P5541_01305"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRV70640.1"
                     /translation="MQESIRDLLRDLPSMDELLQRVWVRPFQSKLGRDAVKSTFADVI
                     GETRSGIIKGKASSLNIAHIEAEVMRRLEKYSRNSLRRVINATGVVVHTNLGRSCLSR
                     DAVNNVAEISGRYSNLEYDLEEGSRGHRNSHVEWLLSVLTGADAAIIVNNNAGAVLLS
                     LAALARGREVVVSRGELVEIGGSFRIPDIMDFSGAKMVEVGTTNRTHMEDYRKAITPE
                     TGMILKVHPSNYRVVGFHTSPSREELAALAREKDLVFMEDLGSGVIADLSYCGLAGEP
                     TVRQCAESGVDLVTFSGDKILGGPQIGAVVGRKQIVEKLRKFPLLRALRVDKMTLAAF
                     ETTMRLYLEGRSGEIPTLRMLTISPADLLKKARSLASKIRRRSGAGVSVVETADAVGG
                     GAFPATDIPGYGVMVSLPGHISDGAAMKILRGKNVPVIPSARDGSLIFHVRTLLEGDD
                     RVISASVGEMLEGGTHDDAKP"
     gene            55893..56486
                     /gene="yedF"
                     /locus_tag="P5541_01310"
     CDS             55893..56486
                     /gene="yedF"
                     /locus_tag="P5541_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRV70641.1"
                     /translation="MVRIDARSKPCPKPVMMTREALEKEGLPLEVIVDSGTALQNVRR
                     FLESRKLSVSVVEEGGTATLICSREGSTSESPAVSNAREKPEDLVLVICSPFLGQPDK
                     SLGDVLMKSFLDTMAGMSSPPSAIALMNGGVMLTLPDNSASESLRAMEEKGCRVLVCG
                     TCTKHFGITGSISTGVISNMFEIAEVMTGQARTLVIG"
     gene            complement(56492..57598)
                     /gene="hemW"
                     /locus_tag="P5541_01315"
     CDS             complement(56492..57598)
                     /gene="hemW"
                     /locus_tag="P5541_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRV70642.1"
                     /translation="MSLYVHFPFCESKCPYCAFYSVRPRKGDMEEYFSSLSLEIDLIE
                     KEMQGFPPVKTLYIGGGTPTIASSGIWKNMVSILENRFRFFQETEVTVEANPGSMTRE
                     QVMIWKDWRVTRVSLGVQSLEDRYLETLGRIHDSRQARQAMERTNSAGLSLSVDMLFG
                     LPDQKMEVWIRDLGEIISLGVDHVSTYQLSLEDGTPWGDFPPTNIPDGYPFYRWAQYY
                     LEKHGFRQYEIASFARPGEWCRHNVAYWFRKDVIGLGPSAWGFIKGVRTGNIRDLRKY
                     SSMLRAGIRPVCVREELDPREQASEAAILALRTRWGIRFERFRKMYGERALEDLLSRL
                     DELPSRLMRRNPWSAVLTRDGMRVGNAVWERLLP"
     gene            complement(57612..59429)
                     /gene="lepA"
                     /locus_tag="P5541_01320"
     CDS             complement(57612..59429)
                     /gene="lepA"
                     /locus_tag="P5541_01320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRV70643.1"
                     /translation="MNVPMERIRNFCIIAHVDHGKSTLADRLLEATTTVLARDMKDQV
                     LDTLELERERGITIKLVPVQMKYRGRDGLDYILNLIDTPGHVDFSYEVSRSLAACEGA
                     LLVVDAAQGVEAQTVANAYLATDQGLEIVPVINKIDLPSALPEQAAHELEEVVGIDAS
                     GAILSSAKEGRGIEEILEAVINRIPAPGGEGEKPLQALIFDSKYNNFRGVISSIRVAN
                     GNIRTGDMITFMATKRSFQVEEIGVFTPAMSPVGELGPGEVGYLIANIKTVEEARVGD
                     TITSQKKPASSPLPGYRKVKPVVFCGFYPIERDDFPQLRDALEKLQMNDSAIFFEPET
                     STALGFGFRCGFLGLLHMEIARERLNREFGVDLVSTAPNVVYQIVDKKGEITEAHRPS
                     DFPEQQFIEEVREPFIKVTSFVPADYVGKVMQLCQDRRGIFGGMDYITPQRARIVYEL
                     PLAEFIIDFHDRLKSATRGFASLDYEHSGFRASDLVKVDLLIGGDPVDAFCFVCHSSF
                     AYNRGHEVVRKLKELIPRQMFEVSLQAAIGKKVIVRQNVKPLRKDVLAKCYGGDITRK
                     RKLLEKQKEGKKKMKQIGKISLPQEAFLAFLKTGDEGED"
     gene            complement(59524..60222)
                     /locus_tag="P5541_01325"
     CDS             complement(59524..60222)
                     /locus_tag="P5541_01325"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRV70644.1"
                     /translation="MRTSASEDRAPYLKERTNDGGSGLYDAAALVLAGGRGKRAGGTK
                     LFLSIDGTPIIEKVLSMARILFPQVILSCRGEDAPTFSRIFAPLFPGLALKIIADRSE
                     GLGPLEGISLGLASSDHPWIFVSGCDMPMVQESVVRFMASKRDPQKDAVIARLGGFVE
                     PLHAFYHKRCVPAVDDAISRGEHRVSSFLPGISAVFVEEEEISHIPGFRRSFMNINTP
                     EDMKRWQEKFFGRY"
     gene            complement(60206..61036)
                     /locus_tag="P5541_01330"
     CDS             complement(60206..61036)
                     /locus_tag="P5541_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRV70645.1"
                     /translation="MSSFKSPVELAKSACLAAKAKVAWSVPQMLVLGTMAGAYIAFGG
                     WLMTVVTHDLAGTMGVGFSKFLGGAIFSVGLMLVVIAGAELFTGNCMMPLSVMAGCTP
                     MSGVIRNWFWVYVANFLGSVLVAFLIYYSGLWKGAIGVHAFKIAAGKMNLPIGEAIFR
                     GILCNWLVVLAVWMSMAATDIIGKIWAIFFPIMAFVASGFEHSIANMYFMALGLLLKG
                     DPSVVSGAGLSGEVLSNVSVGGYLHNLVPVTIGNMIGGIFFVAVFYFLVFRTNLEDLG
                     "
BASE COUNT        14208 a        17808 c        14407 g        14728 t
ORIGIN      
        1 caagaccgag aagggaacag accctctcct cgatcgaggg cgggcaatgg gcatggttcc
       61 gaccgctccg gagatcttgc ccaccgagac agaaacgcgg gaagcactca ggcgggtaat
      121 atccctctgg atctcctcat accagttgag aagtttgaaa ccaaatgaca aaggttccgc
      181 atgtacaccg tgggtccttc ctacacaggc aaggtccctg tatttccagg ccttttcaag
      241 gatgatctcc gcgacctcgc caagatctcc aaggatcagg tcaagtgatc ttgcaagaag
      301 aagagacgag gacgtgtcga taacatcgct gcttgtcagc ccaaggtgaa tgtatcggcc
      361 atcaggcccg accttttctg caacggacga cacgaaagct atcatatcgt ggttgactga
      421 gtcctctatc tgcaggatcc tgtccacatc aatctcggat ctttccagga tgttcaggaa
      481 ggattcctca ggtataactc cctcctccat ccatgccctg catgctgcga tctcgacctc
      541 cagccatgtc ctgaaacggt tctcctggga ccatataaca tccatctcct gcgtgctgta
      601 tctttcgatc aatctatcca gacccccccg agcgaacccg tctcgcgaag ccagtctttt
      661 ctggcttcgt ccaggaaact atcgtacacc ccttcggcaa aatccggaaa ggtgcagtcg
      721 aaggaaaccc atttttttct catgtagcgg agagttacct ccgcaaatat cccgtcgcgg
      781 agatatatcc tgtgtgcgtg atcttttgtt gaagccagga ccagtctcga gccgttcacg
      841 tatccgggat cgatgttgac ctttctgctg tctccgctct gtttttccag gccacaggaa
      901 aaaatcttcc attcggccag cccaccggcc cccgccagtc cactgaaaga aacaaaggtc
      961 ctgtaaagca caggggctat gtttctgtag taatcggtat gaccaaaggg atatgtctcg
     1021 ctttggtact ggatctcccc ccactcgctg gtcaatttcg agatggtcca ttccagcaat
     1081 ccagtgtcag ggtgaagtat cccggctacc cgtttaaccg caggctgcaa cggttctcac
     1141 cgcctttgct atccgaaaga agtccatcaa caaaatcccg ggggttaaaa agttgcaggt
     1201 cccccagtcc ctctccgagt ccaacgtacc tcacgggaag ccccagttcc ctcccgacag
     1261 agaggatcac accgcctttg gcggtatggt catactttgt cattaccacc ccggtgagcg
     1321 ggatcgcccg tccaaaaacc tcggcctgcc tgaaaccgtt ctgtcctgta acggcatcca
     1381 ctaccagcag ggattctgta atccacccat gggagttctg gccaattacc cgctttatct
     1441 tggcaagttc ctccataagg ttggaatttg tgtggagtct gccagccgta tctattatga
     1501 cgcagtcggc accactggcg gcggcggctt tcatggaatc gaatactacc gctcccggat
     1561 ctccccctgg agggtgggct atgacgcgcg tacctgcacg ctcaccccat attctgagct
     1621 gttcggaggc cgcggccctg tatgtgtcgg ccgccgcgag aatgaccttt ctgccctgct
     1681 ccttcagacc gaaagccagt tttgccgctg tggtcgtctt tccgctcccg ttgactccaa
     1741 gaagaagcac cgcagtggca gaaccaccga aatgaacagg ctcaccggtg ccatcaatgg
     1801 cttccagacg ctcacaaaga atatccctca ggagagcaag ggccttttct ccgtccatac
     1861 ctcccgaaga agcaattcgg gaccgcaggt cctctacaat ggagagtgtc aggggaaccc
     1921 cggtgtctcc ggaaagaaga gtctcttcca gatcttccca gaaagagtcc tccggcattc
     1981 cgcaggcaaa aaggcgaaaa aggttccacc tgcgacggac aggctcaaga tcatttctta
     2041 tatcaggcaa tctaaccccc cctgctcctg cttttacgtg ttgcttcccg acgtcgggga
     2101 gttgggcttt ctgggccttc tgcgcctcga cttttttctc gattcggtct tgccctggac
     2161 aggtttctcc gtttccgccc cgggctcttt gccgcccgac ggttttttgt cttcgactat
     2221 tttcgctgaa ggctgagcgt tttttttgcg gttgggacct ccccggcgcc tttgtgatct
     2281 acctgttttt tcgcctggtt ttccctggtt ccttttttct ccctgttttt gtccgggagt
     2341 ttcttttccg gggcactcag ggtcttcaga ttctctctca acaggttcct ggattctaac
     2401 aatctcttcg gaaacattaa cagacccggg ttccacgaca ctctccatgg tccaggtctc
     2461 tcctttcatc accgtttcgc gaaaatattt aaactccgat acaggcacca ggatctctcc
     2521 gttgccgggg cagtaaagcc ggacgatctc cctgttaata tccacaccgg aaactatgta
     2581 gttggcggca ggtgccttga tcttggtccc cgggccagga aggtctttcc agagttctct
     2641 gtagttgttg tgttcaaaac ccatgcagca catgagcctt ccgcatatcc cggatatttt
     2701 ggtgggattg agggccaggt tctgttcctt gaccatccgg atgcagatgg gagcaaaact
     2761 gttaagccag taactgcagc agcaaggcat cccgcaggga gacataccct ttactacttt
     2821 ggattcgtca cgcaccccta tctgtctgag ttctatacgg gttctgaatt cacgggccaa
     2881 atccctgaca aaagccctga aatcaactct ttgttccgca gtaaagtaaa aaaagagttt
     2941 tttcctgtca aggataaact caacatcaac aagtttcatt tcgagatcgt gggatttgag
     3001 cagttcacgc cctttaagca ggatctgctc ttcttcctct tcctgggctt tccgtgcctc
     3061 aagatcctct tcggtagcaa aacggatgaa atttagatcc tgaaaggatg gctcgcctcc
     3121 cctcgggacc ccctctcctc cggtggatgc aaggtctacc cggtaggatt cggcctgctc
     3181 atgagtaatg ggaccagccg ctactgccgt ttcctctccc cttgcggatt ctacaacgac
     3241 cctgttacct gggctgaagg tcacttcgcc attttccaga agtcccaggt atctcggttt
     3301 accaaaaatc atcaaataac tgctcaaacc gcacctctcc ctcaagtccc gcccaaacca
     3361 gatcctgtat catcggggtc gtcaatttgc cctttcgggc aagcatgcat atgctgtcca
     3421 gactggaagc cctgccaaaa tctttttgtc gggcaagaaa cagcacccag ttctcaagct
     3481 ccgggagcat ctcctgcggg ctccttttcc tggtccttcc gacccattta aggaatgtac
     3541 ccgggtttcc cggaatatcg ggtggttcaa cctcgccgga actgcctggt atgaaattga
     3601 aaaccctgat cctgctcctc agggtgggca acatggagtt ttcttccaac aaaatcatta
     3661 tccggcccga tggcgggggc tcttctgtta tttttagcag gctgttcacc gccggaaggg
     3721 acatcctgtt acccgaaaaa agaacaccca gccttcttgt ggaacttacc ggccttaacg
     3781 agagttcttt ccacaaggtc ctgcaaaact caatggtagc ggcttcttcc ggtttcccgc
     3841 tttgtataag atccgggtgg tccttttcgg accatgccgg acgctgttct tcccgcgtca
     3901 tttcgtcgca aagtatcttt cttgcacagg ccattacgca gtcagcatga taagccaggg
     3961 gaagcacaac ggcgaatgcc ggggacgcgt cagaagccgc cgatgccgat gatgtttcct
     4021 ctataaactt ttcgatgctt tcactcagga cctcgtttcc agaaggcaag ggcttttctc
     4081 cgttcctttg agaatattta gttcctgtac actcgatccc tccggactca caggcgtttc
     4141 agtataaccg atgttacttc agaagatata actccgggcg aaagggtccc atctatcctt
     4201 atcacccttt tcggctcgct ttcgtggatt tgtctgtacc cgcaggaaat ccgttcgagg
     4261 agtccgggat ccttttcgaa cctgtcgggt ttttctcccc gttcacatat cctcttcaac
     4321 gccgtttcaa cggggagatc tatccagaag gtgatatccg gtatcgggaa accggccctt
     4381 tgagagaatg tctcgattat gtccctggga aaacctcttc caaaggactg gtaggccaaa
     4441 gttgaatcgg tgaatctctc gcaaagtatc accttgccgg acaggatcga gggccctatg
     4501 acccggtgaa cgtgttcgca ccggtcggcc agaaatagaa gcaactcggt ccaggtattt
     4561 tccgaggatc ctgataatat gagggacctg agggcccccc ctccattcca tcctcccggt
     4621 tcatgggtcc tgactaccct ttccccgcca ataacggacg ctatagccac ttccacagct
     4681 tcggaaagcg aacttttccc cgagccatcg ataccctcaa aggtcacaaa caagacaagg
     4741 cacctccatc agaacctgta aaataccccg gcagaatctc tgtggccgtc atcaagatcc
     4801 aggtacctgt aaaaaactcc ccaggaatca cctatggagt acctgccatc gatagaccat
     4861 cgtccgttct gctggtccca ccactgccct ccaatggaaa agccccccgc cccatggagg
     4921 gggaagagca taaatccgac tcccggatat cctcttaccg aacctgccca gaatctgaac
     4981 atctcaccgg aataaccgat agcggcggaa actcccggat ccgaaccaat gtcctcggca
     5041 cctagcaaaa gacctatttt cctggaacgg ctccccgtcc agagtgaaac atctgccatt
     5101 acatcgctgt ccctgggacc ctgggtaaca ccatggaccg aaacggttcc gtcgaagtga
     5161 atatcctcga tatcggatat tatcctgttg gccttgccga ctatctcccg aatatcgctg
     5221 atagtattct ttcccggagc ttccccttct ttgaagaaag tgtcctcgat ctttctcgtc
     5281 agctcaccaa catttcttgc agcctcgccg atgcggacga ctgtttcgcg aacatcccgt
     5341 ccagacattc catcctcatc gaactgcgaa actacggcac gaagttttcc cataacctcc
     5401 gaaagagtgt tgagcgaaag agacaggctc tccctgttct cctcgactac ggagtcgagg
     5461 ttgcccgtga gccgaacgat gctgtcgcca actcttttga gctgacccct ggcctcattg
     5521 gaaaatccct ggaaatcgtc ggcggcatcg gaaagttttc cgagaaatga attaatgttg
     5581 gaaaaggtat tctgcacatc ccccaaacta cgcctgatgt cctcaagtat ttcgtcgaaa
     5641 gccggcggta tggtaccggg aacaacatcg tcgggagata tgcttgtgaa ggatggcccc
     5701 ctggttatct tgacgcgggg ttccccgaga agaccacctg tatctatctg gaacttgctg
     5761 tctgaaggta ttatgacatg gccttttact gaaacactga caagaacgcc cttgtccatg
     5821 agggctatgc tgttgaccct gccggattcg acgcccgaaa ccagtaccgg ggctcccgca
     5881 tcaagcccca tcgcgtcggg aaaaactatc ccgaaggaat agccgtattg cttgaacctc
     5941 actccaccac tgacgaaaac cagtccccca agaaatgcta tggccaggaa gactacgata
     6001 cctaccctca tctcccggtt catcccgcga tcccccccat gccccggacg gaaatcattc
     6061 cctccgcaaa acgttttacg ctggaatggt cagatgaatg ccaatcagac acggcaccgc
     6121 cccatgctat cttaccttct tccattaaca taagcctgtc agcaattccc aatgctgaaa
     6181 caaggtcgtg agtcaccacc agaacgctga acccgaatct gccgcagaga tcgaccacaa
     6241 gttgatcgat atgtctggcc gttattggat ccagaccggt agtgggctca tcaaggagaa
     6301 ggagcgacgg gctgtgcaca agcgatctgg caattgcaac tcttttcctc atccccccgg
     6361 aaagttccga aggaaacttt tcccggacat ctccgagttc cacgtgatgc agcatttcaa
     6421 gcaccatgga ggatattttt tcctcgtcat ggatgttcct gcaatacctc aggggaaatg
     6481 caatgttctg acccacggac atggaatcga aaagagcccc cccttgaaaa acgaccccaa
     6541 gctctctcct gatgtcgatc agttctttct cttccgttag aggatctatg gtcttgccca
     6601 ggagattgat ctccccctcc tggggtttga gaagtccggc gataagcctc agggtagtag
     6661 tctttccaca gcccgatggc cccatcagac agttgatctc tccggacatg agcctgatgc
     6721 ttacgtcacg gataaccatt tttcccgaaa aaccgacaga tacgtttttc atgttcatca
     6781 gtgagttcat ggtctcaccc cttgataccg ccaaacagca gggtcgaaag aatatagttg
     6841 aagatcagga tcagcatgtt cccccacacc actgccctgg tcgtggcaga accgacaccc
     6901 cttgccccct ggccggccat aaaaccctgg ccgcaggccg agatagtaat cagcaggccg
     6961 aaaacggcag ccttgaggac ccccccgtat atgtctgagg ggttaaggag agcctctacg
     7021 gatcttttga agagatgggg gttgaccccg tgagcggtca cgtaggcaaa ccctcccccg
     7081 atacctatca cgaatgaaaa gagggtcagc atcggaagca ttatcagccc cgcaagaact
     7141 ctcggtaccc caacaaacac gctgggatcg agcccgaaag tcctgagcgc gtcgatctgt
     7201 tcggttacct tcatggaccc tatttcggca gccatcgccg cgccgaccct tccactcacg
     7261 accaggccgg taaggacagg ggacatttcg cgtatcatac tcagggctat aaccccgcct
     7321 atgtagcccg tggcaccaaa ccggacgaac tggtccaggg tctggacagc catgaccatt
     7381 cccgtaaagg cactcgtgat agaaactaca agaacggagc tgacaccgac cctctccagc
     7441 tgctcgagaa ggtcttccct gctccatctg ccgacaaaag tcccgcagat caccctgtag
     7501 ccgaagagcg caaattcacc gagggttgaa aaaagatcaa cagtctgcct accgacccaa
     7561 gagaccaggt tcactgaaga tttaccccct attccgggac aaccctgatg atccttctta
     7621 tcagaccgtc tacgtagtag ttagaccggt aactcctgaa ggatccttcc tttatcatct
     7681 cttcctttat ctggctgagc agtttgcgcc ccgattcctc ctctgcttcc atcgactccg
     7741 gatctcctcc ggggacaaaa ctccggatct cggcaacaac ctcatcgttg gtctccatag
     7801 ccgaaaattc ttcgagaagt tcatcaaggc tttgtatagc cctccagccc tgggcaattg
     7861 aatcctggcc gggttcctct atgccgccgc cccttaccac aacctcacag acgcccctgg
     7921 ctccagccgg tgccttgagg gttatcttct tttttacggt ttccttccgg taaggtctga
     7981 gggtgatctc gacatcgaac tcaccttcgg gcagaacagt ttttttagag aggttgacgt
     8041 cctcgatgaa caccaccctc gggtctgacg ttacctcaat atcgagatgg attcctatgg
     8101 gcattatctc gaagaagggg ttgagtgata tcgcgtcgga aagcattgaa aattctgcaa
     8161 gcatggtctt tccaaggtct gtaggagaaa agaacatatt cgtcctctgc cagccttcga
     8221 caaggccgcc cccttcaatc ttcatggtca gctttgcgct tccctctccc accctgcccc
     8281 agacattatc tataaggcca ttcagcatct ccgggataac cttggtcatg aaaaagggat
     8341 cataggctgt tttaaacctc ttcagggtcc gctctccgga atccacgttg gcaaacctta
     8401 ccgatatatc cataaccggc gcgaactgcc ctattcgccc accgatcgcc tggggcctgt
     8461 cctgggttac tatccctatt atcgatctgg gagtacccag tttgaacggc gatctgtagc
     8521 tgggtatgac ctcgtggatc cacgccctgg caagggggaa ggaaacggca ccccggctgg
     8581 tgaaagggtg ggcaaaggcc acgaaccggc cgtcatttga tactgccgta agagtccctg
     8641 tggccgcaag agagatatct ccccaggcta caagcacact caccgcctcc ccgggcctgg
     8701 caacggctcc aaactctacc gggatgtcgt taccgcggat atcccccggt atcatcacta
     8761 ccggcatacc aaggatctcc tcgatccttt ttgcgctcct tgcgctgtaa cccataccga
     8821 caagcggggc agaaagctcc cggtccattt tatcaccaga atctgctaaa tcggcaggtg
     8881 tttcatcttc ggaaccgtcg gggtcatcat tatcgggatc tttcagttcg aggtccggga
     8941 gcactatccc gggcaccctg tcgggccagt cccagatact gaggatatcc tctatgggtg
     9001 tgaccagccc catctcgtgc tcactgaagt tccatccgta tcctatggca cctatcagtt
     9061 ttcctccaac gaagacgggg ctgccgctca tcccggccgc tattcccccg gttttttcta
     9121 ttaccggacc ggaggccctt atcaggataa ggtttcgcgg cgaggtgggt tcgggaagaa
     9181 cgctgagtat ctttacgggg aatgacacaa cctccccacc cttgacaact gtccaggccg
     9241 ttccctgcat accctcgcga agtgaatcta cacccatgat gcctccaggc accacgaact
     9301 caccggaata gacaaccccc ggaaatgaaa gtaagatcat cgcaacaaca aggaaaagcc
     9361 atgtttttct caaaaacaga acccccaaat agacccagtc atttcccctg cttccggaat
     9421 ctgagatagg actggataaa atcatctatg tctccgtcca gaacggcctg gacgtttcct
     9481 ttttcgaggt ttgtcctgtg atccttgacc agggtatatg ggtgcatgac ataggatcgg
     9541 atctgactcc cccagccgat ctcctttttt tctccctgga tatggttcat ctgttcctgc
     9601 ttttcctgga tcatcctttc gtaaagacgc cccctcagga cctgcatggc gctgtgcctg
     9661 ttcatgtgtt gtgatcgttc gttctggcag cttacgacga tccccgtcgg tatatgggtg
     9721 atcctcacag ccgagtcggt catgtttaca tgctggccac ccgcaccgcc cgacctgtag
     9781 gtatctatcc tgaggtcttc gctggctatc tctatttccg catcatcggg aagttctggt
     9841 gacacattca cggaggcaaa actggtatgt cgcctctttg ctgtgtcgaa gggcgatatc
     9901 ctgaccagcc tgtggacgcc acgctcgctc ttcagataac cgtaagcatg atcccctgaa
     9961 acgtgtatgg tcgcggattt tatcccggcc tcgtcgtcct cgaaaagctc cagtactcgt
    10021 gactggaatc ctctttcctc ggcccacctc agatacatgc gaaagatcat ctccgcccag
    10081 tcctgagaat ccagcccccc ggcgccgggg tgaatactca gtatcgcgtt ggagtcgtcg
    10141 tattcttcgt taagcaacag gaggagacgt ttttcttcca gcgctttttc cagttccgat
    10201 gatcttgcct gaaactcttt ctcgagttcc gagtcatcct cctcacttag aagaccggcc
    10261 agtgtctgca ggtctcccag ttcggtctcg atattcttcc acgagtcgac tttcgcctgg
    10321 aaacgggcga tctcccttgt cacatcctgg gcgtcgtccc tgttccagaa gccctgttcc
    10381 gatgtcgcct cagtcagttt ctccaggtct gccgcagctt tttccgggtc aaagactgtc
    10441 cttcaactcg tatgcaagcg agcgaagtcc ctccataacg tttgatacag gtatctgtgc
    10501 catttgtatt cctcccggag tgctattatt agtatttgca ggcatttcat tataacatca
    10561 cgctcgccgc tattgggcgg gaaaaggaag gcatccatgc gcaaatctac gccaacaaag
    10621 accgctgtac tgaaaaaact atactcctcc attggagaaa ccgtctctgg cgggatactt
    10681 tgccgggaac tggggctttc cagacaagcc gtatggaaag cagtaagatc tctggtggag
    10741 gacggattca ccatagaatc agttccggtt aaagggtacc ggttcctcgg ctcttccgga
    10801 aacgatcttg accctacctg gctggagata cttttatcgg actgcccctg gggtcacccc
    10861 gtacttttct ggcaggagat cgattcgacc cagtctccgg caaaagaaat ggcccgccag
    10921 ggcgctccgg aaggagtagt agtcctggca gaccagcaga cttccggcag aggaaggctt
    10981 ggaagacact ggatatctcc ccctggaggg ggtatctatt tttcagtcat tatccgccca
    11041 gatattccgc ctgaacagat tcagaccctg agtcttgcct cggccatggc tgtacaggac
    11101 gccatccagt cagaatcggg tatcaggtgc cagctcaagt ggcccaacga tattctctgg
    11161 aataattcaa agctgtgcgg aatcctcagc gaggtctcaa gcgaaccggg aaggatccat
    11221 ttcgccgtta ccggcatcgg tatcaacgcc aacatgagag gacaggatat gggtctggga
    11281 aaaggggcca cctcgctggc atcgatcctt gggcacgacg tcgacagggg tctgatagct
    11341 gcctcggtga taagccgttt ccatgacggg gtccgcaggc tggagtcacc tgagggaagg
    11401 atctccctga tatcggatta cgctgccagg tgcgacacca ttgggaaaaa ggtcagggtc
    11461 ctcgtcgatg agagggagat aatcggggag gcatgcggca taggagacac tggcaatctc
    11521 gaagtccggt cagaggggaa gatcctcacg ttctcggcag ctgatgttac ccaccttaga
    11581 aactgtggag accatcagga ggactccctg gagtaatccc atatctgaag gtaaatttca
    11641 tagagaaaca ggatcatatc ctggtcgata tcaggtcgcg gaagttcaat ggacccgggc
    11701 attttcccga ctctttccct gataaatccc ggtgtccatg gatcggtgac gatcagccat
    11761 ttttgcctca cggcctcttc aagcgcggtc tccagaacct cgatcccttt tcccgaggcc
    11821 cagccattct tgacagcatc aggcggcctg acaaccttcc ccgctgaagc ctgaagccac
    11881 cggccgtatt tccaggaaag gtcaagcagt gacttgctcc ctatcatgtt ccttccgaca
    11941 attaccgtgc tgtccagcct ggcctggaac tcggccagcc tcctctgata gtagctatag
    12001 ttggaaggtt ccgaagcgga gagaatatca aggagcctca gcgcgataaa cggaagagca
    12061 gcagggtcaa gaaagttttc gtcccgattg cctccgggga accctttctc gaagaggcga
    12121 aaagtctttg gtctttccag acccccctgg cccagaaacc gtattatttc ctcattgtct
    12181 actgcaaaaa ccagggttcc cacgggaggt tcctttcttg ttttttcctg gacgccgctg
    12241 tcgttccata ctaccagggg acgaacggtc acatgggctc ctccgataaa acgtgcgatc
    12301 atggacaacc acgggtttgt cacccagatc tcagcggatc cggcccctac tgcggatctg
    12361 caaaaaaaca acataaaaaa aaacaccaaa acagaatatc ttctgaaagt tcttcgatcc
    12421 aaaccctttt acccccccgg aatatcctcc ggccatttcc ccgatatctt gaaggatagc
    12481 acaaaaggaa ctggcagcaa ttgcctttat ctggagagtc ttctgcttcc gggttcttcg
    12541 aggggaaggc cctcaccgca caggggacat tcttccggag tgtaaaccgg gaaatcactt
    12601 ttcataagag aaacaaggga gggcccgatg gcattattcc cggaactcct gtcaactaca
    12661 caggctgccc ccagaaactc tccttctgat acctggagga tggcatcgcg gacttccgcc
    12721 gtcgatcttc cggtagttac aacatcttct accacgaaaa aagaaaggcc caaaggggcg
    12781 ggaaaacgcc tgagcttcat aatgcctttt tccctttcgc aaaaaataaa gggcacccca
    12841 aggtatcttg ccgtctcgtg ccctatgatc agaccaccca cggaggggga tattatcaca
    12901 tccggcctga ggtgatctat tctttcccct attcgggcac cagcccaggc ggcgtgctcc
    12961 ggatatctca ggaaaagggc acactgaagg taaaacccgc tgtgcattcc cgatgaaagc
    13021 aggaaatgac cttccaggaa ggctccggat tccttcattc tcctcattgt ctcgacagca
    13081 acagcatcat tcatctggtc tgttcccacc tttccacgcg atccactatc tctgaaagag
    13141 caattgccgg agacggggat ttcagtatcg gtctgcctat caccagataa tccgctcctt
    13201 tttcgagagc ctgctccagg ggcgcggatc ttttctggtc atcctgccct gcctggtccc
    13261 ttatgccagg gacgaccatc tccaggtttc cttccgctat gggtcgcagg agttccagat
    13321 ctgagggaga acagacaatt ccatcaatac cggatgccgc acagatccgg gcccttgatc
    13381 tgagggcttc ttccatcgcg caaccggggt tgacctcttc ccaactggga aggtcgtggc
    13441 ttgtaaggac cgttacgcca aagagtttca tcgggttccc cgacgcgtca cgtgttttgg
    13501 cagcagattc cagcatcgct tttccgccgg cggcatgtac cgtaagggcc cagataccat
    13561 tatctgcaaa ttgcctgact gccatggcaa ctgtgttggg tatatcgtga agtttgagat
    13621 caagaaatac gtcaaagcca atttccatga ctttctttac gaagagcata ccaccctggg
    13681 cgaaaagtcc cggccctatt ttgacgaagg caagttcccg ggcaagaggt tcgatcatgg
    13741 caagggcggc gtcgacatca agaagatcag cagccagaat cacccgcctg ttcttttgtt
    13801 ttttcaacat ttttgagtcg cggctccttt ctctggcctg gcccgaccag tcagggcaac
    13861 tgcgttttca aggctatgtg aatccatgtg ttcgatcaga ccccggcaga taagggatgg
    13921 aagccgcgat tcaacgaaaa gagacgtccc ggcctgtatt gccgatgccc ccgcaagcat
    13981 catcgaaaga gcttcatctg gccctgaaac acccccgcat cctatgacag ggatcgcaac
    14041 gctccccgct gcctcccaga ccatccggag agacagcgga aatattgccg gccctgacag
    14101 accggcaaag atcctgtcga agaagggctt ttcgttctgg ttgtcgacag ccattccaag
    14161 ccaggtgttg cccactacca ccgcgtcggc tcccgcgttt tcacaaacct tcgacgcatc
    14221 tgcaatcgac caggcctgag gtgtgagctt cacccagagc ggtccgttcc aggcggccct
    14281 ggctccagcg acggcttctt ccagactcga gggaagagtc ccccaggaca tgcctccgct
    14341 cgaaacgttt ggacaggaaa cgttcagttc gatcgctcct attttgtcca ccgatttttc
    14401 gagaatatgc actgtatcgt ccagttcttc gatagaacca aaagcaacgt tgactatcac
    14461 cgggaatccg gcggaggaca aagggccaag gtcttcatct atgaaggcgc tgactccggg
    14521 gttctgaagg ccaatactgt taagcatccc cgacggggtt tcaaagaccc tgcccccggt
    14581 gttccctttc ctgggataaa gggtgatacc tttgctgcaa accgctccta caccatccat
    14641 ggatccagtc ggccaaagac ccggatccat aggccataca ccggaagcga tgatgaccgg
    14701 tgtctccata cggactccgc caaccactgt ttccagcctt ttattgccca tcccagacta
    14761 cctctttcca gtcgaatact ggtccctcca cacaggttcg gaccattcct ctccgcgtct
    14821 cgacggaaca ccccatgcaa cccccgatcc cgcaggccat cctgctctcg aggcttacaa
    14881 gggtcttgcc catggggttg atttggccca gactccggag catgccgttg ggcccgcaac
    14941 cccatatctc gcaatctgga ctcaagagag aagatgcagc gtcaattgcc gtacccctta
    15001 ttccaaaact tccgtcatcg gaagagataa ccaacccggg gatcctggag gaacaccagt
    15061 cggcaaataa ctcccaccct ttgccaggca gacccagaac gaaggttttt tcgggctgcc
    15121 caggggaaat atcgaccaga tcaagaaagg gggctattcc gagagtaccc gccacaagaa
    15181 caagttgttt ccctgacgga tctggaaaac cgttgccgca gggaccccga agatccagcc
    15241 tggccccggg gggaagagag gccagaagag aggttcccct cccgacaacc cggaagagaa
    15301 aaccaacctt tttaccggaa gcgaaggtga atgacagggg tctaccgaga agagggtctt
    15361 tcactcccgt caccctcacc ataaagaact gtccggtctt tgtctcgggg atagtttctt
    15421 ccatctcgac tacaacatgc ctgcacccgt tccccgcctc ttcggacaga gatatcctcc
    15481 cggtgagatc ctttgtgccg ttctttccct ggccagatat ggcaaccatc accggaagac
    15541 cactttcccg ccgcaaattg tcatgacagg ccaccccttg agttttttgc catcccaggg
    15601 tgttaacctg gatttgctga accaggtaga tgcatcaacc aatctttcga gtcccggatc
    15661 aatgaccgtc aggtcagccg gggctccagg agtcagggat cccagcgttt tccatctttc
    15721 tggaagaatg gcggccggtc ccgaggtgaa aagggaaagg atcttctcca gagaacaggg
    15781 ttttcccctt ttttcccatt cgtccataac agcggcagcc gcgcattcga gggacggtat
    15841 gccgaaggca gcctcctgaa atggaacgtc cttttcgtcg gtatggtaag gagcatgatc
    15901 ggtagctatc gcgtcaacgg tgccatcctt tatgccttcc caaagggcat ccctgtcgga
    15961 accgcttctc aggggaggat tcaccttgaa gacaggatcg taacctccat ctatgaccat
    16021 ggtttcctcg aggcagagat ggtggggtgt gacgtcgcag gtcaccttgc agcccgaaga
    16081 ttttcctgcc ctgactgcgt ttatagattc ggcggcgctg acatgagtaa agtggagagg
    16141 taccccagtg tcggccgaaa ggctaatacc cctgtagatg tccgccgctt ccgcagcggc
    16201 gggccaccct ttgaggccgg aaagagagga gcatgttccg tcatttacct gtgctcccat
    16261 ggtcagggtc atgtcctcgg gatgctccat aatccttacg ccaaaccttg aagtgtactg
    16321 aagagccagc ctgaggatct tgccgtcact gacgggactg ccatcgtcag taaatagcac
    16381 cgccccggcc agggccattt tccccatttc cgcaagggtt gtccctcttc tctctctagt
    16441 gacacaaccg gccgggagaa ccctggctcc tttgcaccct tctcccttgt cgaccaggta
    16501 acgtaccaga gccgcattat ccaccggagg atcggtgttt ggcatacata ccagggttgt
    16561 aaagccgccg gcagccccgg cgagtgaacc cgaatggaga tcctctctcc actcctggcc
    16621 gggatctcga agatgacagt gaaggtcgat gaaacccgga gaaaggatct tgccggagag
    16681 gtctattacc tcttttgccc ctgatccatc gagcccccct ccgatggcag acactctgcc
    16741 gttttcaacg aagaggtccg ttatttctgc cgaaaatgtt ctcccatcga aaagtttggc
    16801 gttttttaaa agaatactca ctgtttcgca cctccgagac aaacgtaaag aagggccatc
    16861 ctcacagcta caccgcttct tacctggcca agtatcatgc tctgctttcc gtcagcaacc
    16921 tcactggata tctcgacacc cctgttgatg gggccggggt gcatgaccat agcatcgggc
    16981 ctggcatgct ccagaagaga cttatccgcc ccgaataact gatgatattc atcaagagat
    17041 gggaaaagtc cttcgtcctg cctttccctc tgtatcctca gcaggtagat tatgtcggca
    17101 tcattgacag ccatgacggg atcctcttca tagaccgctc cgagggtgtc gacagattta
    17161 ggcatcaggg tcgcgggacc cgacaaagat accctggcgc ccatcttgct gaaagcgatt
    17221 atcccgctcc tggctaccct gctgtgaagc acatcaccga taatggcaac ctttgcgcca
    17281 tccaaggccc cgaacttttt ccatgcggta tacacgtcaa gcagagcctg tgtaggatga
    17341 gcatggccac catcaccacc attgaaaaca acgctttttt tgagttttcg cgcaaggtaa
    17401 tcggcagatc ccaccgctcc atgtcttatc actaccgcgt cggcacccat tgactcgagg
    17461 gtccatgcag tatcacgaag agtttctcct tttgaggtac tggaaccaga cgctgaccag
    17521 ttgacaacat cggcgctgag aaatttctcc gcaagttcaa aggagaccct tgtcctggtg
    17581 gatgcctcga aaaagaggtt gacaaccagt ttgcccctaa gagcgggaac tttttttatg
    17641 ggccggtcga gaagttcgtc catggagcgg gtctgatcca taatgtgctg aatatcatct
    17701 ctttcccact tatcaagatc cagtacgtga cggtgtttcc agttcattcc ttttcttcct
    17761 ctctctcgca tataaccact tcatctttgt tatccagttc cattacccgg acctctatga
    17821 cctcactcct ggaggtcggt acggtccttc caacgtagtc cggatgtatc gggagttccc
    17881 tgtggcccct gtcaaccaga actgccagct gtacagctga gggtctgccc aggtccataa
    17941 gggcatccag cgccgctctt atcgttctgc cggtatagag cacatcatcc accaggagga
    18001 tcttctttcc agtgacatcc tccggaatgg aggtgctgtg aactacaggc tgatcgttga
    18061 gaagcgtaag atcatcccgg tagagggtaa tgtcaagtac tcccgaaggg atccttgtgg
    18121 attcgaaatg ggcaagaata tctctcagcc tgttcgccag gttgacacct cttctttgta
    18181 taccaacgat tatcatgttc tccaagccgc ggtttttttc gagtatctcg tgggacatcc
    18241 ttttaaggac tctttcgata tcctgggctg tcatcactgt tgccttttcg gtgagcacac
    18301 ctttcacctc caaaaaaatg gggcccatag atcacctttc ggtgtccgga ccccttgatg
    18361 aattgcattg caccattccc gtaccgcctt ttgctcacaa ctttacacct ccgtaaaagg
    18421 ccttgcggaa gtgattctag caggttatca aaatatatgc aaccggttgc acgataaggc
    18481 cttttcagcg gcttttcttt cagcttccct tatggtacgt ccctctcctt ccccaataag
    18541 gcctcctgag caaaagacat tgaccctgaa gacaggagca tggggggggc cgcttttttc
    18601 agcaacaata tattcgggtt tgccaagacc attttgttcc atcatttcct ggaggacagc
    18661 cttggcattc cttggaacac caacgttcgc ctcaatggat gacacataat acgaccctat
    18721 gaccttttcc gcagccccgt agccaccatc gagaaagaca gcaccaaaga ccgcttcagc
    18781 agcatcggca catataacag gttgccggct gcctccgctt tgtgagagtc ctcttcccag
    18841 cctcagcagc cccggtatac cggccctgac ggaccaggcg taaagggcat cctcacagac
    18901 gatcgaggct ttcatccccg acatggcacc ttcgtcctcg tcgggaaact ttttgtatat
    18961 catggccgta acacaaagcc ccaacactgc atccccaaga aactccagcc tttcattgaa
    19021 gggacacatt ccgttctcgt ttgaaaagga ggcgtgtgtc agcgcctcca ccaaaagatt
    19081 ccggttttcg aaacggtaat ctatgaaatt ttcgagtttt tcgatcccgg gaatgctacc
    19141 cggaccttca ttgcgggtca aggacataaa tctcctctac ttccggaatt tttcgatggc
    19201 aatgaccccg ttgtgtcctc cgaagccaaa gttgttcaca agaagccttt cgacgttttt
    19261 tttcaaagct ttcccgtcag gaacgacctt gatcggacat ctcggatcgg gcgtaacgta
    19321 gttggcagtc ggatgaacaa caccttcaca tatggactgg atcgccgcaa ctgtttcaag
    19381 agcccccgca gcgcccaaag tgtgccctat catggatttg gtggaattta tcagtatgct
    19441 gtcagcaaga tccccaaaag cgttccttat cgccgctgct tccattcggt cgtttaggct
    19501 ggtggatgtc ccatgagcgt tgataaggtc gacatcctcc ggcttccacc cggccttgcg
    19561 aacagcctga agcatggcct tggcagggcc gctcccgtca ggatccggag ccgtgatgtg
    19621 gtaagcgtca caggttgacc cgaaaccctt tatttctgcg tagatatttg cgcctctttt
    19681 cagggcgtgc tcgagttctt caaaaacaag gaccccggcc ccctcaccca ttacaaaccc
    19741 gtccctttcg gcatcaaatg gtctggaggc atgtgacgga tcctcgtttc tggtagatag
    19801 agccctcatt gcggcgaatc ccgcaacagc caccggggtg atcgcagctt cggctccccc
    19861 tgcaaggatt atgtcggcat catccctgac tatggtgtga taggcctcac ccatagtgtg
    19921 agtcgcagat gcgcaggcag tcactacaca gatgtttggc ccttttgcac cgtatacgat
    19981 ggctacgtaa gccgccggca tgttgctgag catcatgggc acacagaaag gacccactct
    20041 tgatggacct ttttccagaa gagttttcat cccctcgtaa ctggtcgcga tccctccctc
    20101 accgctgccg atataaacgc caaatctatc gggatcgagg tttcctgcat caagagaggc
    20161 atccctgacg gccatttcgg acgcagcaac agaaaaatgg atcaccctgt cagccctcct
    20221 ggcatccttg cggtccatcc attcgccggg atcaaaatcc cggatctcgg cagctatctt
    20281 gaccgtataa tcggaagcat caaaaagcgt tataggtcct accccgttac gcccctcttc
    20341 aagaaacctc cagtaatctt ctcttccgtt tgcaatgggg gagacgaccc ccattccggt
    20401 tattacaacc cttctcaaac cacttcactc cttcggctcc cgaatggcac ttagccactg
    20461 cagggaacgg agtattcggg aaagcgcgga gagacgatct ccccgcgcca ttatcccgga
    20521 tagttcagat tactcttcga ctcccagttt ccccttgatg tattccatgg cttctccgac
    20581 attggtcagt ttttcggcgt cttcgtcggg gatctctata tcgaattcct cttctatccc
    20641 catgatcaac tcgacaatgt cgagagaatc cgcgccgaga tcctccacaa aggaggcatc
    20701 cggtttgatc tggtcctctt cgacatcaag cctgtccatt actatttccc tgagccttgc
    20761 agtgatttct tccattttca tctccttcac ctcctctccg aagaactctt ggcacccgta
    20821 tcagaccatg gtcattccgc catcaacggc aatgacctgc ccatttatgt aagatgcctc
    20881 ctcagaggcc aggaaagcta ccaaagaggc tacatcttca gggctacccg accgtcctgc
    20941 cgggatctga agcatcatct gctctttgac cttctggtcc aggatcgagg tcatatccgt
    21001 ttcaatgaat cctggggcta tggcgttggc cgtgacaccc cgcgagccga gttcccttgc
    21061 cgtgcttttg gtaaaaccaa ggatgccagc ctttgccgca gcgtagttgg cctgaccggc
    21121 gttccctatg agaccgatca ccgaactgat gttgattatc ctgccccatc ttgattttgc
    21181 catatttctt gatgcatggc gcgtcgtcag gaaaactgat tcgagattgg tccggataac
    21241 gtcactccag tcggaatctt tcatcctcat caggagtccg tcacgggtta tccctgcgtt
    21301 gttcacaaga atacccacgg tccccagttt ctcttcgatc tgcctgaaga gttccgctac
    21361 atctttctct gccgaaacat cagcctgaaa ggccattcct tctccaccgg cggaccttat
    21421 ggcttcgaga acctccagtg atccggctga agaggttcgg tagttgatag cgatcctgtg
    21481 accctttgat gcaagtttga gagctatcga ccttccgatc cccctgccag ctcccgtgac
    21541 gagagccacc ctgctttccg gattcatatt attgtccctc cctcaggaaa tcaatcgccc
    21601 ccggaatgtt ttcgcacgta ttcgcggaaa aacattccgc ccagggcgat atccttttta
    21661 tcagaccgga gagaaccgat ccagggcccg cttccaggaa ggcccctgtg ccagctgtta
    21721 tcattttttc cacagagtca gaccacagta ccggcataaa tgtctggaga aagagagcct
    21781 cctgaacgct ttccacgtca ttttcctctt cagcggaaac attagctaca aggggccatt
    21841 tcggggcttt ccaggagcag ttcccgaaag cttcccggag cttgtctgca acaggcctca
    21901 taagagaaca gtggaagggc gcgctcacct tgagtggtag agcccttttg gccccccttt
    21961 ccttgcatag tactaccgcc atgtctacgg ccccggtttc tcctgatatt acgatctgcc
    22021 ccggggcgtt aaagtttgcg gcctgacaaa cacccatgac agatgcctct tcgcacacgg
    22081 caaggacttt ttccctctca agacctatca ccgctgccat agagcctttc cctaaaggca
    22141 cagcttcctg catcagtttc cctctaaggt tgacgaggcg gatcccatcc tccagagaca
    22201 atgcacccgc agcaacaaga gcagtgtact cgcccaggct gtgtcctgca acgtacagtg
    22261 ggtccagatc cagaccttcc tctttttgca gggatcgcag aacggcaatg ctcatggcaa
    22321 gaatagccgg ttgagcatat tccgtctttt taagttcttc atcggggccc tcaaagatca
    22381 gacgactaag tccgaacccc agggctccat cacatctctc gaaaacatcc ctggccgaac
    22441 tgtaacaatc aacaagatct tttcccattc caacttcctg ggctccctgt ccgggaaaga
    22501 ccaaagagta cttcatcaaa atacccccaa agggatcaat taaaaaggtt tttttgcaaa
    22561 accgggagaa cttcgctgat tcccccgaaa agatcgagga ttatatcgcg agccggctgg
    22621 atcctgttca caagtccagc tgattgccct gccattaccg aaccgtaatc tacatcacca
    22681 tccacaacgg cagcccggag tttacctgta ccaagtattt cgatctcctc tacggaggct
    22741 ccttcttttt caatcttttc gaattcacgg gtgagacggt tctggatgca tcggacagga
    22801 tggccagtgg acattcctgt tacgacggta ctgcggtccc tggcccttat tatggcttcc
    22861 ttgtacttcg tgtgggcatt ggactcttca gcgcaaacaa atcttgtgcc cacctggaca
    22921 ccctcagcgc caagagcaaa gaccgcaata acccctcggg aatctgccac tcctcccgcc
    22981 gcaacaacag gtatggagac agagtcaacg acctgcggga ccagaagcat cgtagttact
    23041 tcccctatgt ggccgccggc ctccattcct tcggccacca ccgcatcggc accctgtttt
    23101 tctactcttt tggcgtgagc ggccgatgcg ataacgggaa ttaccttgat ccccagcggt
    23161 ttcagccttt caataacctt cccgggagag cccgcccccg ttgtcaccag agggaccctg
    23221 ttttttgcga caacttccag cgcatcctct gccgtaggag acaggagcat gatgttaacc
    23281 ccgaagggct tctctgtgag cgaacgaacc ttgattatct gctgttcaag aatatcggga
    23341 ggcatactcc ctgcggcaat tatccccaga cctccaccgt tgctgaccgc ggcagcgagg
    23401 tcagcatctg cgacccaggc cattccgccc tggatcaccg ggtattcagt gccaaggatc
    23461 ctttttatcc tgttgtctga aagcataggc tcacctcata tctccagaat aattgcgcca
    23521 tatgtcatac ccgccccgaa actgacgagc atggtcttgt ctcccctgtt tatcttccca
    23581 ccggatattc cctcgtgaag ggccagaaaa accgaagcgg cagaagtgtt gccatatttt
    23641 tgtaggttta caacagcttt tccgaccggg acatggagcc tccgcagaac cccctcgatt
    23701 atcctgaggt ttgcctggtg aaatatccag gaatcaattt cggaaacatc cagtcctgcc
    23761 tgttcgcaaa ggtcttcgac gaaggggggt attatcctat tgacgtactt gaagacctcg
    23821 ttacctttca ttcggacaaa gtgttctccc gcttcgacgg tggcacaagt tgcgggtttc
    23881 gcggctagtc cacccgggaa ctctatgtag tctcccagag cgccatcggc atggagttca
    23941 acggcaagaa ccgagttttt ccccccggta gcacccagca ccacagcacc ggcgccatct
    24001 ccgaacaaga cacaggtgtt cctgtcattc cagtccacga tcctggaaag ggcctcggca
    24061 ccaatgacga gaactttttt ccacaagccc gaccctattc cggctgaggc catggcaagg
    24121 gcgtaaacgc aaccggtgca gccggcctga acatcacagg caccggcccg aaccgcgcca
    24181 atcttgccct gtacgagggg ccccactccg gggaaaaggc tgtccgggga attggttccg
    24241 acaattatca tgtctatttc ttcgggagaa accccggctg ctttcagggc ttccatgcct
    24301 gccctggctg caaaatcgct ggttacctct ccttcggacg caatatgcct ctcttttatc
    24361 cccgtccgct ccgttatcca ctcatccgtg gtatctacca gttttgagag gtcttcgttg
    24421 gttacgattt tttcagggac atacattcca gtcccaagca gtgccactgg taccgcgaaa
    24481 tcaagcgcca atattcttac ctccctctgt cagctcttcg gctatagcct gcacgccgtt
    24541 tctcgacacg aaatctctcg caactctcaa cgctccgcaa atagcactcg ccctggacct
    24601 tccgtgagcc tttattaccg ccccgttgac gccaagaagg ggagtaccac ctgtttcctc
    24661 gtagttgaac cttgaccaga ggtctttcag catgggaagc atgaaggcca taccgatctt
    24721 gggcatgaag cgcctgttga actcatcctt gaccagagtg tatatggctt cacctgttcc
    24781 ttcaataaat ttgagaagta tgttgccggt aagaccttca cacacaacca catcagctac
    24841 cccgaagggt atgccccccc cttcgacatt gccaatgaag ttgagaccgc ttttcctgaa
    24901 gaggtcacgt gcttcaagga tcagttcgtc tcccttcacc tcttcctggc cgttcgagag
    24961 caagcctatg gaagggttct gcacaccggc tatctttctc atgtaaacgt cacccataag
    25021 agcgaactgg tagagattaa gcggcttgca ccgtacagtg gcaccaatat caataagcat
    25081 tgaaagcctg tcgacagcag gaaggggtac cccgagaccg gggcggtcta taccctgtat
    25141 ccttcctacg acaagaacac ctccagcaac gattgcaccg gtgcttcccg ccgaaacaca
    25201 gcctccagcc tcaccggaac gcaccatctg cattgcgacc ctgaggctcg aatgcctttt
    25261 tttcctgata gctacggcag gtggttcatc catggttatc atttcatcag tgtggactat
    25321 atgaagcctt ccgcggatgt cgcccccggc agactcgagc agtggctcga tggaggatga
    25381 ttttccgacc agcgcgatct ccagttcggg aaacttctcg caagccatta tcgcgcccgc
    25441 gcatgtctca aggggagcgt ggtcccctcc cattgcgtca agagccagaa gcacccgtat
    25501 cactctcctc tttattctcg aaagatgcct tgtcacactc tcccaccaca gttatgaacc
    25561 ttcctaaaaa aatttcccgt tcccctactt ttattctcac gcttacgacg tatttgtttc
    25621 ccttttttgt tcccaccttt gcacgggcta caagtctctc gccaactttg gccggatgcc
    25681 tgtaccttac cctggccgat cctgtcactg ccatgtccgc ctcgatcact gcaacagcaa
    25741 gacaactggc cattgaataa atatagtgat cagaaacaaa accggtatgc cgaaaagcca
    25801 tatcacccgt ggtctgaatc gttgagaggg cccattcgtc aggctgtagc tcaaggagtt
    25861 caccgatgat ctcatccctc ttgagagaac gaagcctgct tccagcctgg ctcgccatag
    25921 atcgcatcct ctcgcgtacc tccggtattc cgagaagagc cctgtcaagc cttatcgtgc
    25981 tgacactgac tcccagtttc ccggctatct cctcgtcggt gagaaggggg ttagcttcaa
    26041 ggtttttgac gatcctgccg tgccgttctt tttttccggc ggatcttttc atcgcgcctc
    26101 tcctttgtca tctggttcta gtatcaggtt atatgatcag gcagtataac ccagaagatt
    26161 cctccggtca atggacaaaa aggccggaca aaaaggccgg cggggatctg tttctaaccc
    26221 ggattactcc cctcgttggt cgtcagaacg agagacctcc gattcacaat tcacgacctt
    26281 ccagaaacca gtctcctgac gcaagtgacg caactaatga ctcgtaacgc gaagagaaag
    26341 actaaaaaac ggggggttgc tgccccccgt ttttttcgat tatttctttc tggagatcct
    26401 aaagggagat caggattctt tcttttcctc ttctcccttt gttttcttta cgattatctg
    26461 tcttcccctg tagtaaccgc attcggggca caccctgtgg gactgggcta cctctccgca
    26521 ctgtgggcac agggtggttc ctggggcaga cagcgaccca agccaatggg attttctttg
    26581 gctggttctt ttatgagacg tcctgctttt tggtgtggcc aatctggttc cctcctttcc
    26641 cgggaatgtt ctctcctgtt tcattaattg catccctgag tccggcaagt ttctcaagcc
    26701 tggggtccac cgggccctcg gagcatccgc aatcctccct gtttctgttg gcaccgcaaa
    26761 agggacacag gccagagcaa tcttcggaac ataccacctt ttcaggtagc gatacgatca
    26821 gagactccca gatctggtcc gtaatatcta tgcaggtgtc aagggtttcg acaaggacag
    26881 tgtattcacc ggcctctccc tcttttggtt cggatcccgg caatggctta taaaaatacc
    26941 tgaatttcct cgaaatatca agtggtgttt cttcgagaca tctcgcacac gggaccgaaa
    27001 cagccacagt gagtgccata tccgcgacaa ggcaggatct gtctccgtaa accttgccct
    27061 ccagggttat cactcccggt atggaatacc tctgccccca gtattctatg tcccctccaa
    27121 caggaagaga gcagttaaca tcgacgggag catcggtacg cagggcttca gatccatcga
    27181 aaccgcatat ccagttagcg ggtttttcga gaaaaatcct gttccccttg ctcccttcgt
    27241 ccatggagaa tccctcctaa ccgatcgcca gttccagagt gtctctcgct atgacgagtt
    27301 cttcgttcgt gggaacgacg agtactttta cagcggaacc ttcctgactt atttcggctt
    27361 ctttacctct gaccctgttt ttttcaagat caattttcgc accaaggaac tccagcccgt
    27421 tgcagaccat ttcccttacc gtgacactgt tctctcctat tcccgccgtg aaggctatcg
    27481 cgtcgatacc ccccattgcc gccgcgtagg acccgatatg cttccttatt ccatagcaca
    27541 gtatgttaag ggccagcatc gcccggggat tcttctgcga ggcagcttct tcgacgtccc
    27601 tcaggtcact gctgatacct gaaacgccct gaataccgct ctccttgtgc agaatgtggc
    27661 tcatagattt gggatcgata ccttcctgtt ccatgaggta aaggaccacc gtgggatcta
    27721 tctcgccgca cctggttccc atgagaactc cgggaacggt tccgaaacca aggctagtat
    27781 caacggatct cccgcccttt accgcagaga tcgagcttcc gttgccaagg tggcaggtaa
    27841 cgatctttag ttcctccaca ggcttgccaa gcatctccgc cgccctgaag gccacataga
    27901 agtgactggt tccgtggaaa ccgtaacgtc ttactctctg cttctcataa taatggtagg
    27961 gtaagccgta ggtatatgca taatccggca tggtctgatg gaaggcggta tcaaaaactg
    28021 ctacctgggg aacattcggc aggacctctt tcattgccct tatacccatc aggttggcgg
    28081 ggttgtgaag cggcgcgaga gggacgcact cctctattac gtcaagaact tcctgggtta
    28141 ttaccaccga tcgggcgaat ttttctcctc cgtggacaac tcggtgccca acggccttaa
    28201 gctcatccag gcttttcaga acgccgtgat ccggatcgag aagagcttcg agtactatct
    28261 ttatcccgac cttgtggttg ggtataggaa catccgcttt atgcgggtcc tgacccgttt
    28321 tttcgtgttt gatcctggag ccgtcgatcc cgatcctttc cacgagtcct ttcgcgagta
    28381 ccccttcgtt tttcatgtcg aacaactggt atttcagtga ggaactgcca cagttcaaaa
    28441 caagtacctt catccctgtt ccaccctcct taagtccttg accggaacct ttccagggat
    28501 ttatcgtaca atcagtacga cagggaagga aggttcgggc ccttggcgaa aaaacgtatt
    28561 gtagggatca ttgcggaata taatcccttt cacaatggac atttatacca catgaagcgg
    28621 gccatggaaa ccgcggaggc ccaagccacc gtcattgtgc tttcatccag ctttgtccag
    28681 agaggcgaac cggctttcac ggacaagtgg agccgcacag aaatggccct caggggggga
    28741 gcttctctcg taatagagct cccggtggct ttttcctgcc ataatgccgg ggttttcggt
    28801 tcggcagccg tcgatcttct gttttctacc ggagttgtca ctgatctgtc attcggcatg
    28861 gagaatccgg acgctcccct tgaacagatc gcgaaggtcc ttacctcaga gcccccaaaa
    28921 ttcaggagat ctctcaagac ccttctggct gagggttttt cctatgccga ggcaagatcg
    28981 gccgcagctg acgtggtcat accgggagcc cgagctgttc tttcttctcc taacaataca
    29041 ctggcccaag agtatgtttc aagaatattc gccaggggct acgacttgcg ggctcttcct
    29101 gtaaagcgtc tcggcgaagg gtaccacagc gatatcctcg acccttttgc aagcgccagc
    29161 cgggtgagaa aggcgatccg aagccaggga aaaggggatc cagcgacaga ggctcttccc
    29221 gaatggagcc agcgcattct ggaccgggag atctccaggg gacgatgcct gctttcgctg
    29281 gacctttact ggcgaatcct ttcaacggtc ctgatcaggg aggatcccga aaccatggct
    29341 ggccacgcgg agatgaagga aggaatagaa aacctgctgg cagcaaaggc ccgccgatgc
    29401 ctatcttttg aagatttcgc aggagcatgt accagcaaaa gatatccgaa aagcaggata
    29461 cagagacatg ccgcccacgt ccttttaaac tacggacaca gcctcaacag aagagcacaa
    29521 agagaaggac ctccatattt acgggttctg ggattcgatg acaggggggc ggaaattctt
    29581 cgctcaatga gagaaacatc tcttctgcct gttgtgttcc accccagggg gagaccaggg
    29641 acctatcagc gggagatagc tgaccttgaa tacagggctt ccgccatctg ggagaacatc
    29701 ctttcttcgc ctgaccccgg aagagaatcc agaactccgc cggtcaggcc ggacctggat
    29761 ccttgccgga ggggaacctc tcccgaagtc tcctgaagac accgggagga accatgtcat
    29821 tgattgatcc cccgaaatgg aagacttcct tgacggcacg gctggacagg taagagtatc
    29881 tcgcttctgt aacgataaag aaggtctcta tctcgggagc cagctggcgg ttcatcagcg
    29941 ccagctgaaa ttcgtactcg aaatcagaga gagcccttag tccccttatg atcaccctgc
    30001 attgttcctc cctcatgaaa tcaaccagga gtccctcgaa ttgctttacc ctgacgttgg
    30061 gaagatggct caaggcttcc ctggtcatgg tctctctctc ctctttggag aaggtgtatt
    30121 ttttttgcct gttgttcagt actccgacca tcaggtcctg gaatatagcg gcggcccttt
    30181 cggctatata tatatgaccg ttggtcaccg ggtcgaaaga accagggtac attgccctta
    30241 tcatcaatgt tcaccctttc agcttttttt ggaagatcga aagaaccgtc tcgccgtatt
    30301 gccttgacga cacgatccat tccttccagg aggcgggatc cgggatttct cttttggagt
    30361 gttcaagcac cagaaacccc ccgcccttga taataccaga gagacggctc ttcagggatc
    30421 ccatcctggc agcccaaccg gaacagtatg gaggatccgc aaagaccaca tcgaaaacca
    30481 gacccttgtt accgaaatag gcaaaggatc ttctcacgtc cattctgaga acccttttct
    30541 cgccacctcc ggcccgggtc tctatctcat cagcattctt tcgggacagc tccacaaagt
    30601 acaccgggga aaaccctctt ttgaaggcct caaggccgat ctttccgctc cccgcgaaca
    30661 gatccagaaa tgatctttct tcagccgggc ccaggatgtt gaaaagtgcc tgggccacaa
    30721 gtcccgaggt aggacgtgcc tctttcagga gatatttccc ctttcctttg tttcgtcagg
    30781 accggcagga tcttccagat aacgaagggg ctttatatca agcacggggg tcccgtcaac
    30841 agcgtcaagc cctataaccg caaggacaag accctcaacc cccagaagcc ttacgaagct
    30901 cagccctgtt ccgttgggtc tccgggggct gcaggtagag aaaacccccc tgatcggcct
    30961 gttcaggtca cccctcgggt ggactttcat gctatacccc caggaaaggt gaaacctgaa
    31021 aataattgcc agccgtagct cttcctccag acctgcgaga gcgggcagga atcgatgcag
    31081 caccctgatc ctgctcttaa cccccctgaa ttcgtcaggc ggaacaggtt tctttatcgg
    31141 agattccacg aacccaatgg gggtcatcgg ataaccgttc aacttttgtc acccgccagg
    31201 aattccacca gggatgagaa ggcaagagaa acagcctcgg ttttaaatgg ccgtgagaat
    31261 gaatcaacct tcacgagaca accttcgccc cgagggacta tccttatgaa aaaagtctgt
    31321 ctgaacccct catcggaggt tatagcctcg acaaggataa ccccggatgg gccaataaaa
    31381 ctttccttga acacccatcg gcttgaagcg ctcttcccct ctactccggt caaccggctc
    31441 atgatcgaca aatccgggca tggtgtatct agagatacgt gcaattcttt ctcctccttt
    31501 tttcgcgcag gaagaaggaa cggttccttc ttcctgaacc ggagcaggaa gagatctttc
    31561 ccctgctctc tccacttcct ttcgtatttg gtccttatct ctctatcggg attcaggatg
    31621 gatgattcaa gcgagagagc aggatgtaac ggaagttctc ctgctacctc gtcagcgtat
    31681 tcagggtcat ctgtaaatat ctcgaaagcg cctcccatgg caagagaccc ggccagaaca
    31741 tcggaaaaac ccctgtaggt aaccctcctt ttcgcgtgtc tttttttggg ccatgggcat
    31801 gggaaattca tgtaaacagc atgaagccac tcgggcggga aacattcccc aagcacgaag
    31861 cgggcatctc ccagaacgat ccttgtgttt ttcagacagg atctctggat ccttttaaca
    31921 gcccttgctg tgcactgaag ggagacctct ataccgaata caactccccg cggagatgag
    31981 gatgcgaggt actcgagaaa ttctccgttc ccaaacccta tctccagagc cctgaaggga
    32041 gggtcgacat tagcgatctg gcagattttt ttccggggat cttcgttaat aatgatattt
    32101 ccgaggggga ccaaagagac acctccaggt ttttacacaa agttgttaaa taaaacacga
    32161 tcttcctctt gacagcaagt gtgacctaca tacaatatac atggattatt ctgctgttct
    32221 gtcaaattat tcaaaacact ttttccctct ttttccttat ttcggaacga gttcattata
    32281 aggccaaagg gtttcgggaa ggatggcacc cggcaacagg aaaccggatt ttgtcagccg
    32341 tagggagtgt ggtatcataa aaaatagttt ttaagttttt cttaattaat gcactaacgc
    32401 atctttcact gaaggagggg taatttatgt cagaagtcat cgaagttcga tggcatggac
    32461 ggggaggaca gggagccaag acaggttccg ccatcctggc ggaggttctt ttcgaggccg
    32521 gaaagttcac ccagtctttc ccggaatacg gagcagagcg tcagggtgca ccaatgaagg
    32581 ccttcaacag ggtttcggaa aaaccgatac gcgtccgttc gggcgttctt cagcccgaca
    32641 tagtggttct tatcgatccc actctgatcg acgcggtcag gccaactgat ggcggaaaag
    32701 atgacactgt ttatcttatc aactcaccgg acgatccgaa ggttatcagg aaaaaactgg
    32761 gaatccccgc atcggcgaaa ctcatgaccg ttgacgctac aaagatcact ctcgaggaat
    32821 tcggccagaa caggcctaac accccaatgc ttggcgcact ctcccactgt ttccccgatg
    32881 tcccggtaca ggctttcatt gaccacttca cccacaagat gggcgcaaaa cttccggaaa
    32941 aggttctcac cgcaaaccag aacgctatca ggcgcggtca cgaggaggtg caccagggat
    33001 gagctcactt aaaggctgga aggaccttcc tcccgcaaca accgttcccg cttcaacatc
    33061 cgaggccgta cagaccggcc agtggagaag catgcgtccg gtttgggacg aagagaaatg
    33121 cattcactgt cttcagtgct ggatccagtg ccctgataaa tccatcaagg ccaacgccga
    33181 cggaaaggtc accgggatcg actattttta ctgcaaggga tgcggtatct gcgcggcggt
    33241 ttgccccaag aacgcaatag agatgcggcc tgaagccgaa tttattgagc agtagccctg
    33301 ctgaccgaaa ggatgtgaat cagatgccaa gaaaggatat cacttccgga aactgggcag
    33361 tggccgaggc catgaggcag atcaaccccg atgttgtcgc cgcataccct atcaccccct
    33421 cgaccgacat acccatgagg ttttcggagt ttgtcgctga cggtctcgtg gacaccaatc
    33481 tcgttacagt cgaaagcgaa cattcagcta tcagcgcctg cctcgcggct tcagtttcgg
    33541 gcgcgagggt catgacggca tccagtgcca acggtgtggc gctgatgcac gagatcttcc
    33601 cgatagcggc ctcctacagg accccgatag tcttcggact tgtaaacagg tgcctgggag
    33661 cccctatcaa catccactgc gaccacggcg acagcatggc cgaaagagat tcgggatgga
    33721 tacacatcta ttgcgaggac ggccaggaag tctacgacac tatgataatg gccgtcagga
    33781 tcgcggaaca caaggacgtc ctgcttcccg cttttgtttg ccaggacggt ttcattacaa
    33841 gccattgctt cgagcccgtg gagttcctcg atgacgaaca ggtcacgaag ttcatcggtg
    33901 agagagaacc ccttctttct cttcttgatg tcgataaccc cgttgcctac ggttctcttg
    33961 cgctgacgga ctatttcttc gagatcaagc gccagcaggt cgaggccatg aagcatgtcg
    34021 aaaaagtcta caacgacgtt gcggcagact tcgcgaagat caccggaaga aattatcccc
    34081 tggtcgacaa atacaagaca gaggatgccg attacatcat ggttatcatg tcctccgctg
    34141 ccggagccgc aaaagaaaca gtagacaaac tgagggaaca gggacacaag gtcgggtgcc
    34201 tgagaataag acttttccgc cccttccctg ccagtgagat agttggtgcc ctcaagggag
    34261 ccaagggtat cgcggtatgc gacagggcgg caagtcttgg cgcggctgct cctctcttta
    34321 ccgagatcaa gggagccctt tacgacgcgg gtattcatat cccggcaaac acctatgttt
    34381 acggacttgg cggaagggac ttcttccctc ataacgccga ggaggcgttc atggatctga
    34441 tcaacaataa aatgagcccc gaggaacgtt acctcggcct cagggaatag ggggtgctcc
    34501 aggatggctg tcaaactgaa agaccttatc accaaggaac gggccatgcg gcaggggcac
    34561 aggatgtgcc cgggatgcgg cgcgcctacc gttgtccagc aggcacttct tgccgtcgat
    34621 gatccgatag ttattacttc ggcaacgggt tgcctggaag tttcaacgac aatatatccc
    34681 tacaattctt ggggagtgcc tttccttcac tccgcattcg agaatgcggg tgctaatgtc
    34741 tccggcatag aggcggcgta cgtggccctc aagaaaaagg gcaagatcaa gaagaatatt
    34801 aagtttgtcg cttttggcgg tgacggcgga acctatgaca taggtctgca ggccctttct
    34861 ggagcggcag aacggggtca tgactttacc tacatctgct acaacaacca gggctacatg
    34921 aacacaggag cacagagaag cagtgctaca cccattggcg ccaatacaag cactgctccc
    34981 gctggcaagg tgatccccgg taaaatccag aaaccaaagg atctcaccgc gataatggcc
    35041 gcccatcata tcccctatgt ggcccagacc actatattca atccgatgga agtcatcgaa
    35101 aaggtcaaac gggctataga gaccccggga cccgctttcg tgaatatcct cgctccctgc
    35161 gtccttttct ggcgtcttga gccctcgatg caaaaggaaa tctgccgcct tgccgcggaa
    35221 acccgtttct ggccgatcta cgaggtaata aacggggaat ggaagttgag ctacaagccc
    35281 aaaaaatatg tcccggcaga ggaattcctc aagcctcagg gtcgcttcag gcatctcctg
    35341 aaaggcgaaa caggcaaggc cgtgatcgag aacttccaga aggaaattga cgaagaatgg
    35401 cagtatgtcc tgaaacgttg cggagaaacc tgggagcccc aggcctgagt cgcaccgggg
    35461 tcaaaattta accactttct ttcttgacct tcacggttat cagggataca atgttcgtcg
    35521 cttgcgggac gtagcgcagt ttggttagcg cacttgactg ggggtcaagg ggtcggaggt
    35581 tcaaatcctc tcgtcccgac caataatcca agcgggaggc cttccgggcc tcccgctttt
    35641 ttatcttccc cttcaacggg atggaggatg caagatgacg gaaagattcc acagggcaaa
    35701 tacaaggata ggacaaaatt ttctggtgga taaaaacatc ctgaaaataa tcctggatca
    35761 cgccggaata tcttccgaag acaccatcct tgaagtcggg gctggtaaag gcatccttac
    35821 cagaggcatc ctggagggaa atccccggat gctttactcc cttgaaatag acagatcact
    35881 ggaaccattt ctgaaagagc tgtcggattc tttcagcaat ttcaggatta tctggggaga
    35941 tgctctcagg gaagggtttc ccccctcttt tgatccaacc ccgaacaaga tggtagccaa
    36001 tattccctac cacataacga caccccttat ctggaaggta ctggaatcgt tggcagaaaa
    36061 gggtctcaaa taccttcttc tgatggtcca gaaagaggct gccgaaagga tctgcgcgcc
    36121 agcacgctgc aaggaacggt atcccctggg agtgacaatc cagtcgatgg gcgaggcaag
    36181 aatcctcaga cttgtttccc cagaatcttt ccgccccacc ccttcggtaa cttcggctct
    36241 ccttgagata aaccttctaa caagggaaga tctgcccaat tgccagctct ggagaagact
    36301 ccttcgctca ggtttcgcac aaaggaggaa aactctcgtc aataaccttg gaagggattt
    36361 tccggaactg aagacaaaga tcgcgtctat tatcaattct ttgggattcc ctgttactgt
    36421 gagggcagag gagcttgagg ttgaggaatg gcatgatctt ctccgaatat ttcgaacagt
    36481 cctggcaaca aaaaagccgg agacgtctgt gacgcatccg gcccgaaatc gacaaccata
    36541 aaataagttc tcttcagcgg accctgtcct tgagagcctt acctgcacgg aaagctggaa
    36601 ctttctttcc aggaatattg atgaccttgc ttggatcctg gggatttctg ccctttctgg
    36661 gagcccttga acgcacctcg aaggtaccga agcctaccag ttggaccttt tcgccctttg
    36721 agagagattt aacgatggtt tcgaaggtcg acgcgacagc ctctccagcc tgttttttgc
    36781 ttatgcccgc agactttgca actgcattga tgatatccgc cttggtcacc ttacgcacct
    36841 ccatccttct tgttttccgg ttcgcgaaaa aaatcaggaa catttgaacc cgtacttaat
    36901 ctataccatt tactgcctgt ccggtcaagt aaaaacccct atattgtcgc aaatttctag
    36961 gttttgctct tacttctcca gaacagtctc aggggagtcc cctcaaaatc gtcaagttct
    37021 ctaagttttt ttgctacatg cctttcaaag gcctttgtta ctatggctgg atcgttgacg
    37081 ataaagacga aggcgggagg ccttgcggca acctgagaac aacttttcat cctcagtggc
    37141 atcccccggg ggccgacggg caacctctca aaggagaggg tatccctgag catagtgttg
    37201 agcctggccg gatccatcat cctgctcctg ttggcatgga cgcgaaaaat ggtctccggg
    37261 agtttgtgca ctccccttcc cgtaagtcct gatataaaga caacgggggc gaaccccacg
    37321 aatggcatct cgtctctgag tttttccgtc accttgtctc caagttccgg agtcttggtc
    37381 agaaggtccc acttgttaac ggcaagtatc agtcccttgc ctttttccag cacaagtccg
    37441 gccatccttt tgtcctgctc tgtacagggc tctgtcccct ccattacaag gaccgcgaca
    37501 tcgcatcttt ctatgctttc atgggtccgt acaagggagt agtattccac gtcagagctt
    37561 atcctgcttt tttttcttag ccccgccgtg tctataagcc gaagtggcct gccctctatc
    37621 aagacccggg aatctattga atccctggtg gtacccggaa cggggctgac catggatctt
    37681 tcggtcccgg agagaagatt gaggagcgtc gatttcccta cgttaggtct tccaacaatt
    37741 gctatcgcta tctcgtcggg attcctgagc gaatcgggat cccctggagg aagtagagaa
    37801 atcacctttt caacaagttc atcggtgttt cttccgtggg ccgcactgat cccgaaaacg
    37861 tccccgaaac ccagcgcatg agcctccatt actcttgatt cgtgaaccct gtcatcaacc
    37921 ttgtttgccg caaggacaac atgctttccg ctttttctta gaaaaagggc gatctcccgg
    37981 tcaaggggcg taactccgtc cctggtgtca atcaccatta tcaaaacatc actctcatcg
    38041 acagccagtc ttacctgtct ccggatattt tcggtgatgt catcccctcc gtcagggaaa
    38101 aggccacccg tatcgaccag aaggaatctt cttccgtcct gttccacctc accataaatc
    38161 ctgtccctcg tcacgcccgg ctggtcatcg acgatagatg cccttcttcc tactatcctg
    38221 ttgaaaagcg acgattttcc gacgttaggt ctgcctacaa tagccacaat ggccatccct
    38281 ggatcattcc cttcctaatc tgcccaaacc tctatcctgg ggaatcccct gcctgatctg
    38341 gatatgtgaa aaagaggggc gagagctttc ctgacccttg acaggttccc gctccttgcc
    38401 catagcatat cctccaaagg gtccgggtca agacactcaa tccctgaagt ggcaaggaga
    38461 tccctcgtct cggctttcat tcctttgagg cccctgatct caaccaggta tctccaggga
    38521 ggcaggccga gttccttgcg ctccatgagc tcggctctcc agaagtggtc ccaacccgct
    38581 gccaggccct tctgccaccc tgaccctggc accctgctct ggataaggac cgttcgaaaa
    38641 ctgttgcctt ttcccctcca ggctgactcc cagaccatgc ggaaagcccg gctcctggca
    38701 tcgtaaaagg gggtgttaac ggccatatct gcatccagcc agcatacaag ccctatctcc
    38761 agatgatcac agagctctat cgccttccgg gatccaacga caaggccccc ggaagcaagg
    38821 ccttttacaa ggtcctttct tgctgaaacg gtctgcctga tatctgcatg ccaaatatat
    38881 acggggcaac tatcacccag aacattctcc ccaatatgcc tcagactctc caggccgggc
    38941 ttcatcccct ggagcgatat ggctccgcag aacgggcaaa cttcggccag ggctgtcttt
    39001 ttgccgcaga atccgcacac aagggcttcc tctttatcat cccacctgac ggggagtccg
    39061 catgaagggc aaactatcat tcttccgcac tcactgcaga taacggcccc ggcatatcct
    39121 ttcctatcca gtatccacag aaccggccgg ccccccgaga cttccgcctg ggtacgttta
    39181 agggcagaat cgcttaccag caggggagac tggatccccc ggacacttat ctttctggcc
    39241 atacccatat cgacaaacac cattcgcccc gttgcctctc gtgggcactc ccggggcatt
    39301 cggaaaaaac tcctcgacga agggaccccg ccaccggtaa ccagggtcgc tccccatgac
    39361 tgggctctgc tgccggccgc gctccgggcg tgaataaagg gaaacctgcg cgaaagatat
    39421 gaagggtttc cttcgtcctc gacaataacg gcggaaacag acgttagcgg agcaaatagg
    39481 gcggccggag atccgaccac cagccggaag tttccccttc ggaccctttc ccaccggctg
    39541 tcagaagccg gcccgggtgc agaaagccat aaaaggctgt cttttttgtg tttttcggga
    39601 agttttttcc agaaagcgga agcggcggat ctgtccggaa aaagggcaag aactccgccc
    39661 cgggaggatt ctatcacctc caggtaccgg tcagatctcg cagaaaaatc cggcgagtca
    39721 catcgctctt cgttgaaatc tccacgggga ccatccatct cgggaagacc ttcgaaagac
    39781 ttgccggaaa tgacagaagc aggcgcgacg gccttcaggg cctctccgaa accacagaga
    39841 aaatgcttgc ctatcttttc ggatgtattg aagagttccc accccagggg tgatcgatca
    39901 tctatcgcct ccagggcaga aagcaccctg tattcgccga aaggccgctc catggaagac
    39961 gatccagtgg caaaagcaac ccttacgccc cgtcccacag ggaccctcag tctggctcca
    40021 cggctgcaaa cacggtcaaa ttcatatgta agtgtatgcc accatggacc cggtacgaca
    40081 acttccagga acaaattcag agacccgccg cttcaagttt tttaagaacg atcccccata
    40141 cggcgtccgc aacttcttcc ttggtacccg atatctcttt ctctattcct tcttttccta
    40201 tcattaccac ggaattatac tctgagccaa aggcagaagc atcgccactg acactattag
    40261 ccactatcag gtcaagtccc tttcggacaa gtttggcaga cgcgttggca acagcgtctt
    40321 ctgtctcagc cgcaaacccg accaggtatt gtccaggcct cttcgaagca ccaagagatg
    40381 cagcgatatc agggtttggt gtgaactcaa tggtaaggtt cttttccttc tccctcttga
    40441 tctttctgtc cattaccctg gatggagtaa agtcacccac cgcggcggcc ttgacaataa
    40501 catcataggc cggagcttcc aacatcaccg catcgtacat ctcaatagcc gaaacaaccg
    40561 gtataaccct gatgccatat gggacaggct gggaagacgg gccagagatc atcaccacct
    40621 cagctcctct gtaccatgcc gtgcgggcaa cagcggaccc catctttcct gagctgggat
    40681 tgcttatgaa ccttacagga tctatggcct ccctggtagg tccggaggta accagaacgc
    40741 tcacaccatc aaggtcagcc gaaggacaga cagccctgaa ggtctcttcc cggataacgt
    40801 cgatggatgg aagccttccc ttcccctcgg aaccgcaggc cagctctccc gattcaggca
    40861 caacgagatg gtagccaagt tctgaacact ttttcgcatt atctctcacg gcagagtgtt
    40921 cccacatgtt tacattcatt gcgggaaata taagaactgg acaccgcgcc gccagaaggg
    40981 aggcatgaag aagtgtagaa ccatcaccga aagccgcgcc atgcagaaaa gacgcggtag
    41041 ctggagcgac gatcataacg tcagcccatt ccgcaagaga aatgtggggg attttccatc
    41101 ctctttcagc tgaaagaaaa tcatcctcca gccaaacccg cctggaggaa agagacgata
    41161 tcgagagagg ggaaacaaat cgctcagctg atctggacag gatgacctct acttcccagc
    41221 cgtgcttcct gaactccctc actgtttcag gagccttgta tgcggcaatt ccgccggtta
    41281 ccccaagaag aagtttacga tttttcttcc aatccatcgg cattatcggc cgcttctttc
    41341 tctgccacgg cgggagaggc cgattccatt ccgaagatgg acacctttcc ctcgctgatc
    41401 tccctcatcg ccatttcaat aggattggat ccttcgtccc cgaagggcat acgccccttt
    41461 ttctcactca gctgccgggc ccttttggag atagcgcagg ccataagata tttgtttgtt
    41521 atgggtctgg aaagatccat tccatctgtt tttttcgtca caatgcaaca cctctgccct
    41581 gatattttaa ttatcttaat ctcctgtaac tctccactat tccagcaagt tctcctagcg
    41641 ccctgctgaa aaaatcattg acaataacat gatcgtacat tccggaaagt tcgatctcgt
    41701 gtcttgcgcc ttcaatcctg gcttgaactt catcaacgct ttctgttccc ctatggctga
    41761 gacgttccag cagatccttc tcggaggggg ggagaataaa cagtaaaacc gaatcaggca
    41821 ttgcctcctt tacctttacg gctccctgga catcaatctc cagcacaacg tccctgccct
    41881 tcgaaagaga tgcctccaca tcctccttta gtgtcccgta gaggttaccg tgaacttccg
    41941 cccattccag gaacataccc tcttccgcga gtcgcctgaa agacccggga ctaataaatc
    42001 gatagtcaag accgtccttc tcccccttac gcggggacct cgtggtacag gatatggaat
    42061 attcaaggtt gtctaaagta ttgaaaagtg cctttctcag agtccccttc cccaccccgc
    42121 tgggaccaga gacaacgaaa aggcgccccc tagcgatccg tctcacactc cctgtccctg
    42181 ccctcgaacc ggacaccaat ggtttccggc tggatcgccg agagaactac gtgatcgctg
    42241 tccataacaa gaatggccct tgtctttcta ccctcggtgg catcaaccag cttgccctgg
    42301 gccctggctt cgtctttcag tcttttgatg ggagccgacg aagggtgtat gatcgcgact
    42361 accctttcgc tgacgaccat atttccaaaa cctatgtgca aaaggttata tcccatcttc
    42421 tcactccacg ttctgtatct gttcacgaac ggattcaagt tcgcttttag cttctaccga
    42481 aagccacctt atttctgcat cctgaacctt cgaggcgacg gtgtttatct cgcggttcat
    42541 ttcctgaacc agaaaatcaa gggtctttcc gttaacaccc ttattatcta ccatggaacg
    42601 gaatttatcc atgtggctcg ccagtctcgc cagttcttcc gatatgtccc acttgtcgtt
    42661 gagtatgaca atctcctggg ccagacgacc ttcatcgcag tcaaaacctc cggactcgac
    42721 aaaggatatt attctttcct tcatcgatga gagtgcaccg gcttttgcgg ccttccacct
    42781 gttctcgatc tcgatcaccc gactctccag cccttccagg tgggcaagga tctctttttc
    42841 gaggtggctc ccctcagttt ttttcatgtc gacccattcc ctgatacctg cagcaaccag
    42901 agacagaata gcctcttcga tctctttttc gagttcttcc gtctcctggg atgaaaccat
    42961 gacccccggt aacgagagta gttgttcaac ctggacatcc tccggaagac caaatgcagc
    43021 ccttgccttt gctgtgtctc ttatgtagcc gtcaagaaca attctgtcta ttacagcccc
    43081 cctgaaagca gggccccaac atacttccag cctgacctgg atctttcctc ttcttgaggc
    43141 ttttcttatc tcctgctgga tggtttgctc gaaacggaaa agttccctcg ggcttttgac
    43201 aaaaatttcc tggttcctgt ggttaacgct ggatatttcc agggaaatgg atccaaaagg
    43261 tctgtcaata ctggtcctgc tgaaaccagt catgcttctg agcatcttca tccagcccct
    43321 ttccggtaac atagccgaaa aatttttcga tattttcaag cagtgtacca atgttgtctc
    43381 cgcgtgtggc actgacggga atcacaatat cctgggtcct tcccacctcg gcgatcctcg
    43441 acgacgcttc ttcaggttca agaaggtcga tcttgttcat aacgaccatc ctgggtattt
    43501 cttccgcccc tatcgactgc agaagggtct ccacgactga tatatcatca ttgaaacccc
    43561 caaaggaagc gtcggctaca agaagaacca ggtcggaacc tgagacctcc tccatagtgg
    43621 ccctgaatgc ggttacaagg tcatgaggca gattcctgat aaaccctacc gtgtcggtca
    43681 tcagtatgaa agaaccgcca gggagcctga ccctccggac aaagggatcc aacgtggaaa
    43741 agagcctgtc ctcagacaca aggcttttat cgccggaaag gcacttcagg agagatgatt
    43801 ttccgctgtt cgtgtaaccc gccagggcca cggtacgaag cccggcgcgg accctccgtt
    43861 tcctctgggc ctgtcttctc tcccggataa tttctatttt tctgctgatc tccctctcac
    43921 gtctttcgag ttttctcctg tgtctctcaa actccgtttc tccaggcccc ctcgttccca
    43981 atccgccacc caatctggac atctgcatgc caagaccttt caggagaggt atctcgtacc
    44041 tgcagcgagc aagctctatc tggagtttcg cctcacctgt ctgtgcccgc tgctcaaata
    44101 ttttcattat taccaagggg cggtcccaga tcatcagtcc cgtgatctct gaaaggtgcg
    44161 ctgcctgggc aggagacagc cttccgtcaa ccaccagcag cgacgcaccg gactccctgg
    44221 caagaagggc gatctcctgg gctttgcccg acccgatgaa ggaatctgga tccggtttat
    44281 cccttctctg tatcatttgc cccgcggtac ctgtccccag gttttcaaga aggagtgaaa
    44341 gctctttcag actggcatcc agcccctcgg gatctttacc ggagatcgca acgaggatcg
    44401 ccgtactcgg ccaccccttg cctctatcgg tcaaccgaca gttccccttc cagttttctc
    44461 agggaatttt caagtttttc ggtaattttc tgtctcagct ctttgacggg gacacccttt
    44521 atccctgcag gatatatggg atccccaaag accaacctta tcctgacggg tcgataccgt
    44581 gaggactccc tgggaagagc ctcgaaagtt ccatgaacat atgccggcac caccggcacg
    44641 ccggtcttgg ccgcaaggag gccggctcct ccttccagtg gctgcaggct cccgtcaggg
    44701 gaacgctgcc cctcggggaa aagcagaaca ctctcccctt tgttgatcct ctccagcaga
    44761 aacttcaatg ctcctgcagc gccttgcagg tcatcccttt tcgcagggac cgctcccagg
    44821 gaccttatgg caaaaccaaa aggaggaaag gaaaagagtt cggatttggc aagatatctc
    44881 aacctcctcg gaaaggcagc acctataacg atggggtcaa ggttgctgca atggttggcc
    44941 gcaataatta tcggaggttt tacagatagc gccctctcca gtcctgtaac ctggagcctg
    45001 ttgtagacca gcagcattat tcgaaaaaaa cctgcaacaa gccagtaaaa aacagatttc
    45061 attttatccg ctcctctctg gccggccgga cccgccaatc acgggggata ccagggccat
    45121 gatccttgtt accacctcat ctacatcaag gcccgtcgta tctatgatgt gggcgccttc
    45181 gggaaccata aggggggaaa cttccctctc cctgtccttg cgatcccgtt cccggatatt
    45241 ctcaagtatc tccccgatct caggtgtttc tcccctggcg gaaagttcca gcatccttct
    45301 ttcggctctt accgagacat ccgcatcaag atatattttc agggctgcct ccgggaaaac
    45361 aacactcccc atatcccgcc cgtcggcaac aatgtctgac atggcagcct gctccctctg
    45421 aatgccaatc aggaatctcc ttatcatggg aagggccgca taagacgata ctattctgtc
    45481 tatcttctgg gatctgatct gatccgatac gtctttaccg tccaggatga tcttgccctt
    45541 ttcaagtcct atcctgcaac aagctagaga ggaacaaaga aaatcgttct cgacgggtgg
    45601 aactgaattt cgggaaaggc aataggcaat agtcctgtat atcgctccgg tgtccaagta
    45661 ctgaaaaccg atcctcccgg caaggagcct ggcaatggtg cttttcccgg ctccggcagg
    45721 tccgtctata gttatgaccg tactgtcggc atggtcacta cttatcattt ccagagatac
    45781 acctcccaat ctaaacggtc cctcctttga gtatcattct ttcgagttca tcaccgctga
    45841 tccttacgat cgctccggag cgaatctttc ttaactccag atgtccgatc tttctcctta
    45901 taagcctcat gaccctgaat cccgccgctt tggccataac ccgaacctct cttttgacac
    45961 cttcggacaa tgttatggag agccatcgtc tttctggttc tccggctacc agttcaacat
    46021 tcaggggtat aagcctcttg ccttcaatgg tcgtacccat gcgaagtttt tcgataccgg
    46081 cctgatcagc ctctttctcc aggagtacct cgtaggtctt cccgatccct tgttccggat
    46141 ggatgatctt ctgagcaaag gctccatcat ttgtgagaat gagtagacct tcactatcct
    46201 tgtcgagcct tccaaccgga aaaacaccag cctcaactgc atctgccgga gtaatatcta
    46261 tcaccgtcgg atagtatttg tccttcacag cacaaacgat cccgacaggt ttgttcatta
    46321 ccaggtaaac ttcaggtttc gggacaacaa caagaccatc caggcataca ttatcacccg
    46381 gccttatcct ggtagaaagg tctctcacag ccattccgtt gatcgagacc ctcccagacc
    46441 gcacaagatt ctcgacgttt cttctcgacc ctgcgccact cattgcaagg taacggttca
    46501 gtctaaaaaa acccttgtca acatcattca tcgatcccat ttgagttccc ggcctcgtcc
    46561 tcaggcagga ccatctcctg catctcctcc agagttggaa gatcggatat cgccccaagc
    46621 ccgaatattg aaaggaagga gtcagtagtc ctgtaaagaa gcggggtccc cgaccccttt
    46681 ttccttccgg cgacccgtat tagtccatgc ttcaaaaggg tctccagaac cctttcgcat
    46741 cttacccccc tgatctcctc tatctcggaa cgggtaacag gttgagcgta ggctattacc
    46801 gcaagcgact ccagagcagc tttgcttagc cggaccttct gcgtaagagc cacatcccga
    46861 aaaagggcta cgacctcctc ctgttcgggg gaggtcgcca tcatccagcc tcctgcaata
    46921 tttaacagac agagaccatg gccactgcgg tatctcccgg agatctcatc aagtgcacgg
    46981 accacctgtc cgtcggtaac acctgctacc gaggcaagct cacgaacaca aactggctgg
    47041 gtcgccacaa acaggactgc ctcgaccgac ctcgccaccg gagaaagggg acgttcagca
    47101 gtgtattccg acatctccaa acatctcctc ctgggaaatc cttataatgc ctagcctggc
    47161 catctcgaga agcgcaagga tggtaactac aagcagcggc cttgttgaag atcttacgag
    47221 gatgtcgcta agaaacacaa acccttttcc tgagaccagt tctcggatct cgtccatcct
    47281 tacctcaacc tgaccttctt cgggtgtcgc tacgggtatt ccatcccact cgatgttctc
    47341 gagctccctg ccctgacctt cagatctttc cttcttttct gatactattt cccaccagac
    47401 catggagagg gaataaagat ccccaaggtc aaaccaggga ggcccctcct cgtgactgtt
    47461 ccttgtaaaa cagcgggccc ttgccctcat aagttcgatc atgtgagctg atgcccttct
    47521 gtagggcatg tacctgccaa gagcatcaaa gagatcctca ccttctggaa catcttcgat
    47581 ctccggcttc tcctctccgg gaagcgcagg aagaagggac ttaagtttgc ccagcaacaa
    47641 cctggccgaa agagagaaga aggccgccat ttcgtttatg cctgccctct cgttttttag
    47701 caaaaaatca taatagacgc ggatcagttc actgacgctg atcctggagg cctgcacctc
    47761 ccccgattcg accagattac agaggagatc caggggtcct gaaaaacctt caagttctac
    47821 ttcaaatatc attaatactg cctcccggcc ggggagataa aatcactacc tgtcaacaaa
    47881 ccctatcgca tctatcacct gcgacattgt cgccgaggca acatctctgg cccttccggc
    47941 cccgctacga agaacctcaa gaacaccatc ctcttttaat tcgaatgatc ttcttctatc
    48001 ctgtatcggt ccgagcacat ccatgagatg cccgtaaagg gctttcttgc agtcgaggca
    48061 accaattccc gcgcttctgc agccatcggc aagcctttcc ttttctccgg cgtcggggtt
    48121 aaagaccttc tgtatgtccc ataccggaca ttttgcggga tcccccgggt ccttgcggcg
    48181 ttctcttgcc ggatcggtca tcatggtacg aagtttctcc cagatatctt cgggggcatc
    48241 ggcgatattg atggagttgc cgtaggattt actcattttc ctgccgtctg ttccaggaac
    48301 cttcggggtt tccgtaagca gtgtccgggg ctccgggaaa atatctccgt aaaaatgatt
    48361 gaaccttctt gctatctccc tcgagagttc gaggtgagcc gactggtcct ctccaaccgg
    48421 gaccagatca cccttgtaga gaagaatatc cgcagcctgc agcacaggat atccaaggaa
    48481 agcgtaggtg ctcaggtcct tgttttgcat gttgatgatc tgttccttgt atgtaggatt
    48541 tctttcgagc catcctaatg gagctatcat agaaagagca aggtgcagtt ccgcatgctg
    48601 cgggacatgc gactgaagaa atatggtgct cctttctgga tcgagcccga caccaagcca
    48661 gtcaagcagg acctcacgac agttcgccct gacggagccc gtatccgcat actccgacat
    48721 tatcgcgtgc cagtccacaa tacagtaata gcactcatac tcttcctgaa gccttaccca
    48781 gtttgagaga gccccggcca gatgaccgag gtgcagcctc cctgttggcc gcatcccgct
    48841 gaaaaccctt ttcttcatac cctttcacct cgttgtccag ttgatttcgt aatttctccc
    48901 gggtcgaaac cccttttatc aacttcgata accttcaccg gaagaaagcc ttcaagcatt
    48961 tttctcaaaa cggggatgga gagccgctcc atctctccat ggtccacaac tactacaccg
    49021 agaccgcttt ccacagcatc caccagttga tgatacttca tatcggcagt tataaaaacg
    49081 tcagcaccct tcgaaagggc aaggggccac atttcccccc ccgatcctcc gacgagggcg
    49141 atcctggacg gcgatttcgg gagattataa gacctcaccc atgagagttt ccaggagtca
    49201 caaagaaagt ctcccagatc tttctctgga atccccgcct tcaggtcccc gcaaagcccg
    49261 atcccccagg agccttcggg gccatcttcc agcgggactg gcccggcaag acccagtttt
    49321 tcacctagca aggcattgac cccacagggg ctgacatccc agttggtatg tgccgcaata
    49381 accgaaatgc gatgatccag agctttcagt attttgccgc cagggactgt ctgtgtctgg
    49441 atacttctca acggatcaaa aaccattgga tgatgcacaa ccaggcagga acatttctgt
    49501 ttaatggcct ccgacaaggc ctgctccgtt gcgtcaaggg agaccgctac acctttaact
    49561 tcccattcag gagaacctgc ctgcaaaccc ggattgtccc actcacagga ccaggcaaaa
    49621 ggagcaaatt cctctatggc tcttatcaca tcttttgttc tcgtcattat cgatcacctc
    49681 tgaataaaaa aaataaagcc gcaccgggcg gctttattca attctctggt gggcccacct
    49741 ggactcgaac caggaacctt ccggttatga gccggtggct ctgaccagtt gagctatggg
    49801 cccgaaaatg tttggtagat cctgtgtagc gggaatcaac ccggaaaaac tattcctcga
    49861 cggcaataca gcctaccggg caactatcgg aggcctcacg ggcacattca gccccactct
    49921 ggttaattac ctttgcctta cctgcattct cgtcaagttc aaagacttcg gggcaaatct
    49981 gcgcacaaac tccacagcct atgcactcgt cacgattgac tttcacgatc atggagacct
    50041 gccacctcct cccaagtgga gattctaacc cacttaagag gggacgtcaa acaggaaaaa
    50101 caggggcaac aggaaagggg ggcaagtgaa gccagaataa cgaaacaccc cggtcgggaa
    50161 tcagaaaata aaaacacctc aacggtaaaa cttgaaccgg taacggtgcc ggaaatcacg
    50221 cattttagaa tttgtccgac ttcaagcctg gaccttgact tcagggatac cgtcgctacc
    50281 cctctcgagc ctcaggagag cgctgtactc tgatacagct tttctggccg gttccgacgc
    50341 ggctatgaaa accccggtcc cgacaacctc ggctttgaac tcccgggcaa ccagcagcat
    50401 tcctgcgaca gtgcttccac ctctcataaa atcgtcgata ataagaactt ttttgccggc
    50461 cgacaaaaac cttgtcccca tgaacatggt tctgacctca ccgcttccag agggataatg
    50521 gacagcaaca gctggtccat cacttggccg gtttctgaaa cggcacactg ccatcggaat
    50581 gcccagggcg tgggccgtaa aaaccgccag ggggataccc tttacttccg atgtcattac
    50641 aacatctggc tccctgtccg caaaatcaga ggcaagagca taacccaaag gccctgcaaa
    50701 accggggttg aacagaatat ccgagtaatg gatcagcatt cccggaagga ttctgttgtc
    50761 ctgggaaaga gtctcggcta tcgatccaag aaaagacttt ctgtactctc gggaaagttt
    50821 cggcctgaga aaagcccctc cgcttcttcc cctttccaca cagatcccgc caataccttc
    50881 ttctttcatc gatcggtcta tgatcgccac atcgtcactt atgaccgtct tagagacctc
    50941 gaactgttct gaaagtcccg tgagagatat ctgcctggag ggagcgacca gaaaacggga
    51001 agccattctg ataagtcttt cagtacgggt gcttttcata tttttcaccc cagatcgaaa
    51061 atatgccttg caacatcgtg ccttttgaac agttcaaccg ctttcatcaa tcctgcacct
    51121 tccctgaaaa ggcagaaaaa tgaactaccg cttcctccaa gaccccaggc aaacgagcct
    51181 gaggaatccg cgatcctcgc aaaatcgtgg tattccggat gcctcgcact tataacaggg
    51241 aaaaaatcgt tcggaagaag cccggtcttt tcttttcttg caaggattcg ggctatgcgt
    51301 aaggcttctt cccgcgcatc cttatcgctc attggccagc cggaggatct gtagagatca
    51361 tcggcaaggc ggtacgcctc ggccgtattc tgagaccaac acggcacgac aacaagaacc
    51421 tgggggctaa ttttttcgac ccctcgcagc ggagatggaa tgtccccgat cccgcttctg
    51481 tagccggctc tttcccctcc aaaaagaaag ggtacatccg cacctaatga gagtatctct
    51541 tcttcggaaa attgtacaaa accctcatct gtcaggaacc ttgccagggc agccgcgttg
    51601 ccgctgccgc tcccgatacc actgcctgga gggatttttt tccaaagatc tatctcgaaa
    51661 aaaggcaatg cagaggattt tctcctggca attttcaaca ctttgtccag aatgtttttg
    51721 cctcttatcg agaaaccgtg aaccctgagg atatgttcac ttacattatt atggtcattg
    51781 atgctcaatg tcaacttttc agccgccggg agtcgcaaaa agagggtccc caagctatga
    51841 taacctccct gcaatgatcc cgtgatcctt agggtcaggt ttatctttgc aggagaaacc
    51901 aactcacgca gcaccggcac cacctcgcaa aaaagaaagc cccggcgctc gagcaccggg
    51961 gcgtttacgc ttgccttgtc ctagaactgg atcagcttct tgttcccagg aagtatctcg
    52021 acttcgacct cacgggtgag aagttcagca tagctgaagg atacggtact cctttgagac
    52081 tcgacataca tggtgaacag gttgggatat gtctccatga taacacccat tcgaacctct
    52141 gttttacggc ggcctttcaa agccttgtag cgcaccctgg agcccttgta ctcgttcacc
    52201 cgatcccgga tcgtggaaag gttaggactc atatgtgtca ctcctgtatg tagtttgaaa
    52261 ggattttacc acatatttcc gggatattca atagacgggc tgataaaatt aaacatagat
    52321 tacatctagc gatccggatt attcatccat caagtcgcca gacttacttt ccttataata
    52381 tttcgttttg ccattcactc gagtcagggg ttacgtggcc ttcaaacctt tgttttcctc
    52441 aggatccgct tatcctggac cctccttgtt attcccatgg aatcgagcat ctccagcatg
    52501 gggagaacgt acttgcggct gctgccggtc agatcccgaa cggaggctac cgtaatatcc
    52561 cctcctgccc cggcgagttt ttctaccagt ttttccagaa cgttcctcga caccagaaac
    52621 tcgcccccgg ctagcgttat ctgaccctgg gctctcaatg cctccaatga tctcgcgatc
    52681 tccgcggcag aaagggctgt cccttcggat atttcatcca gggtcggcgg ctggaattcc
    52741 cttcgagagc aatactccag gatcgttctc cccgaagcgc ccaactggga tgcatccctt
    52801 ggtgtgaagc ccggcatgct gactctctgg tcgtccagag ccacttcccc attttccacc
    52861 catctgtcca ggatccgttt ggaatatttg atatcgatct cgggaaacag ttttctgcag
    52921 agaacatcag caaccatacc aggattctcc ggttcctttt cgtgcattcc atcgagcagt
    52981 accttggttt ccgcaagctt ttcacgggcg tattcacccg aaataacaag cccttcgcct
    53041 gtttcaatat agatcgatcg ccctttacgg tccgcctctt tcgcaatcct ggcaaattcc
    53101 gagggcactt cctgaagcag gctgattgct tctctgacgg gaagcaactt catgtgagaa
    53161 gaaagctctt tgaatctttc ttccgtatct gctgcggttg aaattttctt taaaaaggca
    53221 agatgtgata ttttctcttt atgtcccctg ggccttcttg aaagaggcat aacaactgtc
    53281 cctcctccga tggtccttag tggactgtaa aaccttatta cgaaagcctc atccctggta
    53341 cataccaccg gctcctcggc tacgatctgg gcaaaagcgg tatctccggg ctcgagtttt
    53401 tccctgtcga aaaaagatac acgccccaga agatcggcgg tccccaggtg catcctgacc
    53461 ctctgccagt gttcaactgc ttcgggtgac gaaggaagta ttgaaagccg cacgtcgaac
    53521 ctttccgtgg cgctgaaaac acccggagtg cagagaacgt cccctctgct gatctcgtct
    53581 agtgatattc ccccgaggct catggctatc ctttgtccgc ctgtaccagt ctcaacggga
    53641 acatcatgga cctgaacgga tcttatctcc gtctttaacc cgaggggcaa aagttcaagg
    53701 ggatcatcct ttctcacact cccccgatac gccgtacccg ttacaactga accgaacccc
    53761 ttcacaggga aaaccctgtc aacaggcata aaaaaccccc cgccggaatt cctggggctg
    53821 atcttgccaa ccataattcc gattgactcc ataagcttgt ccagattaaa tcccgatacc
    53881 gccgatacag ggataatttc cgctcctttg aggaaagtcc cctctatttc ctccctgatc
    53941 tcttccagag caaggtcaag aaaatccggg tcgaccatgt cggctttcgt tacaacaact
    54001 atgccatgcc tgacgccgat cagtccaagg atatccagat gttcccgggt ctgtgccctt
    54061 accccctcgt cggcagcaat tatgaataga accccgtcga taccggccgc gcctgcaacc
    54121 atctgcctta tgaatttttc gtgccccggc acatcaacta tgcttacgac ctggcctcct
    54181 ggaagggtta ccggggcaaa acccagttcg atggtgattc cccgacgttt ttcgtccctg
    54241 agccgatcgc agtcaacccc ggtcaaggcc ttcaccaggg tggtcttccc gtgatctata
    54301 tgccccgctg ttcccaggac gacaggaacc tcacggtttt gcgtcatcat gggttccccc
    54361 ctcgagcatc tctcctacac tggcggatat gaccctgtca tcgccttcca gaagtgtcct
    54421 tacgtgaaat atcagggaac catcccttgc tgaggggata acgggaacat tcttgccacg
    54481 cagaattttc attgcagcac catcagaaat gtgtcccgga agggaaacca taacaccata
    54541 gccgggaata tccgttgccg ggaacgctcc tcctccgacc gcgtcggccg tctctacgac
    54601 agagacccct gctccgcttc tcctgcggat cttcgaagcc agtgacctgg cttttttaag
    54661 aagatcggca ggggaaattg tgagcattct cagggtgggt atttcccccg atctgccctc
    54721 aaggtaaagt ctcatcgtcg tttcgaaagc agcaagggtc atcttgtcca cccggagcgc
    54781 cctcagaagt gggaatttgc ggagtttctc cacgatctgc ttccgcccga ctacagcccc
    54841 gatctggggc cccccaagga tcttgtcgcc cgaaaaagta acaagatcca cgccggattc
    54901 tgcacactgc ctgacggttg gctctcctgc aagcccacag tacgaaaggt ccgctatcac
    54961 accactgccg agatcctcca tgaaaacaag gtctttctcc ctggccaaag cggcaagttc
    55021 ttccctggac ggggaggtgt gaaaccctac caccctgtag ttggaaggat ggaccttgag
    55081 gatcatcccc gtttccgggg taatggcttt tctgtaatcc tccatgtggg tgcggttcgt
    55141 ggtgccaacc tcaaccattt tggcccctga aaaatccata atatccggta tcctgaagga
    55201 acctccgatc tcgaccagtt ccccgcgaga aacgactacc tcccgccccc tggccagcgc
    55261 ggcaagggag agaagaacag ctccggcatt gttgttgact ataatcgccg catcggcacc
    55321 cgtcaggaca gagagcagcc attcaacatg cgaattgcga tgtcccctgc tcccctcctc
    55381 aaggtcatat tccagattgc tgtaccttcc tgatatttcg gcaacattat tcacggcatc
    55441 acggctcagg cacgacctgc cgagattggt gtgaaccaca actccggtcg cgtttattac
    55501 ccggcgcagg ctgtttctgg agtacttttc aagccgcctc ataacctcgg cttcaatatg
    55561 ggcaatattc agtgatgacg ccttcccctt gatgattccg cttcttgttt cccctatcac
    55621 gtcagcaaat gtcgacttga cggcgtccct gcccaatttc gattgaaatg gccttaccca
    55681 taccctctgg agaagttcat ccatcgaagg gagatcgcgg agaagatccc tgatcgattc
    55741 ctgcatcatt tcaccttccc gaaaaatggc ttgatatgca tatgttagtt tatcccaatg
    55801 aaggtaaaaa cccaacgggg catgactgtg agccgatgat ggtaatatga tccatatagt
    55861 gccgaatatt ccgtaaaggg aggttgatcc cgatggtaag gatagacgcg cgcagcaaac
    55921 cctgcccgaa accggtcatg atgaccaggg aagcccttga aaaagaaggg ctgccccttg
    55981 aggttatcgt ggattccggg acagcactgc agaacgtaag gcgattcctg gagagcagaa
    56041 aactctcagt ctctgttgtg gaagaaggag ggaccgcaac actgatatgc tccagagagg
    56101 gcagcacctc cgaaagcccg gcagtgagta acgccaggga gaaaccggag gacctcgttt
    56161 tggtgatctg ctcccctttt ttgggtcagc cggacaaatc cctgggtgac gtcctgatga
    56221 aatcatttct ggacaccatg gcgggaatga gcagcccccc ttcggcgata gcccttatga
    56281 atggcggagt tatgctgacc ctgcccgata attcggcttc agaaagtctg cgggcaatgg
    56341 aagagaaagg gtgcagggtc ctggtttgcg gtacatgcac aaagcatttc ggtataacag
    56401 gttcaatctc gacaggagtg atctcgaaca tgttcgagat cgcagaagtg atgaccgggc
    56461 aagcccgcac acttgtgatc ggatagcccc gtcagggcag aagacgctcc cacaccgcgt
    56521 tgcccactcg catcccgtca cgggtcagca ccgccgacca tggattcctt ctcataagcc
    56581 tggaggggag ctcgtcaagt ctggataaaa gatcctccag ggctctttcc ccatacattt
    56641 tccggaacct ttcgaaacgt atcccccacc tggttctgag tgcaagtata gcggcctccg
    56701 aggcctgctc cctcgggtcg agttcctcgc ggacacatac aggtctgatg ccggccctta
    56761 gcattgacga atatttccga aggtcacgta tattcccggt ccgcacccct ttgatgaagc
    56821 cccatgccga aggaccaagc ccgatcacat ctttcctgaa ccagtaggcc acgttgtgcc
    56881 tgcaccactc accgggtctg gcaaaacttg ctatctcata ctgcctgaaa ccatgttttt
    56941 cgagataata ctgggcccac ctgtagaagg ggtatccgtc ggggatattt gtcggcggga
    57001 aatcacccca tggagtccca tcctcaaggg acaactgata ggtcgatacg tgatccacac
    57061 caagggaaat gatctcacca aggtccctga tccatacctc catcttctgg tcgggtaatc
    57121 cgaaaagcat gtcaacggaa agcgataacc ctgcggaatt ggtcctttcc atggcctgtc
    57181 tggcctggcg ggagtcatgg atccggccca gtgtctcgag atacctgtct tcgaggctct
    57241 gaacgccaag gctcactctc gttactctcc agtctttcca gatcatgacc tgttcccggg
    57301 tcatcgaacc tggattcgcc tcgacggtaa cttccgtttc ctgaaaaaaa cggaaccggt
    57361 tctcgaggat cgagaccatg tttttccaga tccccgaaga ggcaatggtt ggtgttccgc
    57421 cacctatata aagggtcttc accgggggga acccctgcat ttctttctcg ataaggtcta
    57481 tctccaggct caatgaggag aaatactcct ccatatcacc tttcctgggt ctgacgctat
    57541 agaaagcgca atagggacat ttagactcgc agaagggaaa gtgcacatag agcgacaccg
    57601 tcgggacttc tttaatcctc accctcatca ccggttttga gaaatgcgag gaaagcttcc
    57661 tggggaagag atatttttcc gatctgtttc atcttcttct tcccctcttt ctgtttttcc
    57721 agaagttttc tcttcctcgt gatgtctcct ccgtaacatt tggcaagtac gtctttcctc
    57781 agaggtttta cgttctgcct gactataacc tttttgccga tcgctgcctg gagggacacc
    57841 tcgaacatct ggcgggggat cagttccttg agtttcctga ccacttcatg tccacggtta
    57901 taggcgaaac tcgagtggca cacaaaacag aaggcatcta caggatcccc accgataaga
    57961 aggtcaactt taaccagatc cgaggcgcga aaaccggaat gttcgtaatc aagtgaggca
    58021 aatcctctgg tggccgattt gagccggtcg tggaaatcga taatgaactc ggcaagagga
    58081 agttcgtaaa ctatcctcgc tctttgggga gtaatgtaat ccattccgcc gaatatgcct
    58141 cttctgtcct gacatagctg catcaccttc ccaacgtaat cggcaggaac aaaggacgta
    58201 acttttatga aaggttccct tacttcttcg ataaactgct gttccggaaa gtccgagggg
    58261 cgatgcgctt cagttatctc gcccttttta tctactatct ggtataccac gttgggagct
    58321 gtcgacacga gatcgacacc gaattcccga ttgagtcttt ccctggcgat ctccatgtga
    58381 agaagaccca ggaaaccgca ccggaatcca aatcccaggg ctgtggatgt ttcgggttca
    58441 aaaaagatcg ccgaatcgtt catctggagt ttttcgagtg catcccgcag ttggggaaaa
    58501 tcgtccctct ctatgggata gaaaccgcaa aaaacaacgg gcttgacctt gcgataacct
    58561 ggaagaggtg acgaggcagg ttttttctgg gatgtaatgg tatcaccaac cctggcttcc
    58621 tcaacggtct tgatgtttgc gataagataa cccacctcgc cgggccccag ttccccgacg
    58681 ggactcatgg ctggagtgaa aacaccgatc tcctcgacct gaaaacttct tttggtcgcc
    58741 ataaaggtga tcatatcacc ggtcctgatg tttccgttgg cgacccttat ggacgatatc
    58801 actccgcgga aattattgta tttcgagtca aagataagag cctgcagggg tttctcgccc
    58861 tcaccaccag gggcggggat cctgttgatc acagcctcaa gtatctcttc aatcccacgg
    58921 ccttccttgg cgcttgaaag gatcgcacca gaggcatcga tccccacgac ctcctccagt
    58981 tcatgggcag cctgttcagg caaggcggaa ggaaggtcta tcttgttgat caccgggact
    59041 atctcaagcc cctggtcagt tgcaaggtag gcattggcaa cggtctgagc ctcgactccc
    59101 tgggcggcat ccactaccag aagcgctcct tcgcaggccg cgagagacct cgatacctcg
    59161 tacgaaaagt ccacgtgacc cggcgtatct ataaggttga ggatgtaatc caggccatcc
    59221 ctgccccggt atttcatctg gaccggaaca agtttgatgg ttatgccccg ctccctttcg
    59281 agttcaaggg tatcaagcac ctgatccttc atgtctctgg ccagcacagt agtggtagcc
    59341 tccaggagcc tgtcggccag cgtggatttc ccatgatcga catgagcaat gatacaaaag
    59401 ttcctgatcc gttccatagg cacgttcatc gatatacacc tcatcctgag aaacgccaca
    59461 tattttaacc acgagaaggg gaaatgtcat tgatggtttc agtttcgggt gtcgcaaaaa
    59521 cggtcagtat cttccgaaga atttctcctg ccatcgcttc atgtcttcgg gagtgttaat
    59581 attcatgaag gatcgcctga aacctggtat gtgggagatt tcctcttctt caacaaaaac
    59641 agctgatatt ccaggcagga aagacgacac cctgtgttca cccctcgata tagcatcatc
    59701 caccgcaggg acacaccttt tgtgataaaa ggcatgaaga ggttccacaa acccccccag
    59761 ccttgcaata acagcgtcct tttgaggatc tctctttgag gccatgaaac gtactaccga
    59821 ttcctggacc ataggcatat cgcagccaga aacaaagatc caggggtgat ccgaagaggc
    59881 cagacccagg gatatccctt ccaggggacc aagcccctca ctccggtctg caatgatctt
    59941 gagcgcaagt ccggggaaaa ggggcgcaaa gatccttgaa aaggtcggag catcttcccc
    60001 ccggcaggag agaatgacct ggggaaagag aatccgggcc atggaaagaa ctttttcgat
    60061 tatcggagtt ccgtcaatgg agagaaaaag cttggtgcct cctgctcttt ttccgcgtcc
    60121 tcccgcaaga acaagggcgg cagcgtcata aaggccgctg ccgccgtcgt tagttctctc
    60181 tttgagatat ggtgctctgt cttcgctagc cgaggtcctc aaggttcgtc ctgaacacca
    60241 ggaagtagaa tacggccacg aagaaaatgc cgcctatcat gtttcctatg gtcacaggta
    60301 caaggttgtg caggtaacct ccgacggaaa cgttcgacag gacctcgccg gagagacctg
    60361 ccccgctcac aaccgaggga tctcccttca ggagaagtcc gagagccatg aaatacatgt
    60421 tggcaatgga gtgctcaaaa cctgaagcga caaaagccat gatcgggaag aagatggccc
    60481 atatcttgcc aataatatca gtggcagcca tgctcatcca tactgccagg acaacaagcc
    60541 agttacagag tatgcccctg aaaattgcct cccctatggg aaggttcatc ttgccagccg
    60601 cgatcttgaa ggcatggaca ccaatggcgc ccttccacag acccgaatag taaatgagga
    60661 aagccacaag cacactgcca agaaaattgg ccacgtagac ccagaaccag ttcctgataa
    60721 ctccgctcat gggggtgcag ccggccatga cgctcaacgg catcatgcag ttgccggtga
    60781 aaagctccgc gcctgctatg acaacgagca tcagtccgac gctgaatatc gcaccgccca
    60841 gaaatttgga aaagccgact cccattgttc ctgcaagatc gtgagtcaca acagtcatca
    60901 accagccgcc aaaggcgatg taggcgcctg ccatggtgcc cagtaccagc atctgaggaa
    60961 cgctccatgc aacttttgcc tttgccgcaa gacacgcgga tttcgcgagt tcaacgggag
    61021 acttgaaaga actcattaac cggattcctc ctcctgggga tttgccccta tggggccaag
    61081 gattcttcgg accagccttt taagccagcc gttagcgatt ctaacatgga acgttatttg
    61141 tgtcacgaat t
//
LOCUS       DAOTIQ010000004        55050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_585_length_55050_cov_7.366015, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000004 DAOTIQ010000000
VERSION     DAOTIQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 55050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55050
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_55050_cov_7.366015"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..354
                     /locus_tag="P5541_01335"
     CDS             151..354
                     /locus_tag="P5541_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRV70646.1"
                     /translation="MTTQGTVKWFNDAKGYGFITTDDGKDVFVHFSAIEGDGFKSLAE
                     GQAVSFEITEGSKGPQAANVQKI"
     gene            complement(477..1430)
                     /locus_tag="P5541_01340"
     CDS             complement(477..1430)
                     /locus_tag="P5541_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV70647.1"
                     /translation="MKKIFALALVMVMALAAGASAQTFVSLATGGTGGTYYPIGGGIA
                     DVVSKNLSDVQMTAETGNASQANLNLIGTNQIEMGLVQNDVAAFAYEGKYMFKEPYKN
                     VRAIASLYPETVQAIAMKKSGVASMRDLPGKRVSVGAPGSGVQVDVSLIFKVLGYKYS
                     DVNADFLDFNNTTQRMKDGQLDVGFVVAGFPTASIMDLATMHDITLIEFDDKLIEDLS
                     AQFPFFVKDIVPAGTYRGIDKDIQTVAVQAMWVCDGNLSDDVVYKITKAFWENIEDVH
                     KVHAKAKMITLDTALNGISVPVHPGAAKYYAEVGMTVPEIK"
     gene            1735..2298
                     /locus_tag="P5541_01345"
     CDS             1735..2298
                     /locus_tag="P5541_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HRV70648.1"
                     /translation="MRLKDFLGSEKKSGAEISRDRLEEAPPVAERFNPDEKDMKSKMN
                     LQVSITLSDDSQIGRSALLLASTTGPGEEKRLKDQIVDFGWKAVATEVGGLAGELPQK
                     ITRAVVGAALNGEIVRKTSSEMHGLMHAAFEAMNGFMSIGMLEASIGVKIGIVRNRTW
                     IAVAVVGDTAYHAVAHHERCGLGVMHI"
     gene            2415..2654
                     /gene="rpmE"
                     /locus_tag="P5541_01350"
     CDS             2415..2654
                     /gene="rpmE"
                     /locus_tag="P5541_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRV70649.1"
                     /translation="MKKDIHPEYGKCKVSCACGNSFETRSTVEEIKVAVCSSCHPVYT
                     GKRGTRVSEAGRVEKFQKKYAGTRFGAPAEEEQKG"
     gene            2676..3368
                     /gene="thyX"
                     /locus_tag="P5541_01355"
     CDS             2676..3368
                     /gene="thyX"
                     /locus_tag="P5541_01355"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRV70650.1"
                     /translation="MPISVLLVASTPEPDMTVAAAARLCYSDASAGDLLEKMRGEDIS
                     RFIGHLRGSGHFSPFEHAVFTFAIEGVSRVCTHQLVRHRMASYSQQSQRYVDMKEPEV
                     VIPPSIISDPTALDIFESSVRQSHTAYAAMVEVGIPREDARFVLPHGWGTKIVVTMNA
                     RELHHFFSMRLCRRAQWEIRDLAREMLRLAREKAPNLFAVAGPDCVTRGRCFESRPCG
                     QPFANVEEVLSE"
     gene            3365..4312
                     /locus_tag="P5541_01360"
     CDS             3365..4312
                     /locus_tag="P5541_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRV70651.1"
                     /translation="MKRLSTVFLRLFSFVLSPALLSREVPVGGQAVIEGVLMRGPYRW
                     ALAVRSPDGGIWNRCWGSRPWTSSRPWNFPVLRGVATMAEMLVTGFRAISLSAQVSLG
                     EEEKITPLELAATFAVAIIGVVGLFIILPVWISDLGVAWLGTGSAGAHILEGTARAAV
                     FIGYIALIGFWKDIARVFGYHGAEHKTINAWESEASLDPQTVSKYSRIHARCGTSFLL
                     VVVAVSVVVFAAAGHGSVLWRISSRIILLPLVIGISYEIIKWCAGNRSLGGLLMAPAL
                     WLQYLTTREPDEGQIEIAISALNLATGDGTCAESIGGGE"
     gene            4316..5395
                     /gene="prfA"
                     /locus_tag="P5541_01365"
     CDS             4316..5395
                     /gene="prfA"
                     /locus_tag="P5541_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRV70652.1"
                     /translation="MDKDLAKKLESLEKMMEEIESRMSDPSVASNQGERQKLGRKHAE
                     LSGVVEVYREFRRLSEDMAGARGLLHSGDHEMTELAREEINDLEPKLEELESQLRILL
                     LPRDPNDDKSVIVEIRAGTGGDEAALFAGDLSRMYGLFAENNGWKTELLTSSETGIGG
                     FREVVFRVDAHGAYSQLKFESGVHRVQRVPVTESSGRIHTSTATVAVLPEAEDVDVEI
                     RQEDLKIDTYRASGAGGQHVNMTDSAVRITHLPTGLVVTCQDERSQIKNRAKAMNFLR
                     AKLYDLELREQQAKTAAERKGQVGSGDRSERIRTYNFPQNRVTDHRIGLTLYRLESMM
                     EGDLQEMISALVMADQAEKMKAAEG"
     gene            5422..6255
                     /locus_tag="P5541_01370"
     CDS             5422..6255
                     /locus_tag="P5541_01370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HRV70653.1"
                     /translation="MAARELRSAGVDRPWYEADLLVGHLTGLDRALLHTRPHFPFPST
                     LVPGLLEALSRRKAREPLQYITGECDFMDMNLSVGPGCLVPRPETELLVLETRKFFSK
                     GVFMDWGTGSGCLASAVLSFCPESRCVAVEREGLALMWAWKNLHKLGLLDRCRLWHSK
                     DPWEIPVPSSSLEMIISNPPYIASEEIDSLMPEVSAYEPRPALDGGVDGLDPYRILIP
                     YSLEKLRPGGVLALETGDDRGAREIEIMAKGLMTLESRVEDIHGIARVLVFRKPCSAT
                     M"
     gene            6306..7487
                     /gene="trxB"
                     /locus_tag="P5541_01375"
     CDS             6306..7487
                     /gene="trxB"
                     /locus_tag="P5541_01375"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRV70654.1"
                     /translation="MEKRELVIIGAGPAGLTSAVYGKRSGLDVLVLEKGVPGGQINAT
                     DEIENWTGVIHASGAELADSFRKHAEHFKAEFRDAEVTGIELCDGCKLVKTGDGDIEA
                     EAIIIATGASFRKLGVPGEAEFTGRGVSYCAVCDGAFFEEQEIAVIGGGNTAVEEAVY
                     LTQFADKVTIIHRRDNFRADRVAIERAMTNPKIEVVWNSVVEEIAGEDMVEKVVLKNV
                     RTGVVSDLPVSGVFVFTGMDPNASFVKGVVESAGNGWIVTNERMETSVEGIFAAGDVR
                     EKFLRQVVTAAGDGATAAMAASSYVSEQLHIQSLLLEPEHVTAYIFSSIDEGQARLAA
                     DIEKISSASKVVQIDGYKNKRMVEKLGIARLPSLVEFKGGKITRSEFAQDAESVKKFL
                     S"
     gene            7554..8486
                     /locus_tag="P5541_01380"
     CDS             7554..8486
                     /locus_tag="P5541_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRV70655.1"
                     /translation="MIVTVTLNPAVDEEFIVPEFRPGKWFRTSNVNRSPGGKGINVSY
                     MLSQAGYQSVAMGFLAGFNGEYVRDALRRMRITTNFVHVQGDTRANVYIVDEVGHVET
                     GIAEGGPYVPDEALARFLANYERMLGRARIVVLGGSLPPGVPQDIYRDLVDRAKSRGI
                     PTLLDAAGPPLMTAIESGPTFARIDHRFMARVAGVSLTSLDNLIEVVSRVHDYGVEWA
                     VASYRSYGAVFFAPEGIFLAEYERRGLVSLFGADDALVAGFIMAREEKMDTEGTIRMA
                     TACAWENSLHVDKGLRSRSEIEQLIPQVKVVKLD"
     gene            8483..8983
                     /locus_tag="P5541_01385"
     CDS             8483..8983
                     /locus_tag="P5541_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRV70656.1"
                     /translation="MKGDVGDNRIIMFVCSGNTCRSPMAEGFMNRCLEDISGDLPFTA
                     CSSGTMASPGLPANPMALKAMQEHGIDISRHRSSPTGIWIPPEETLFFGMTRVHRREL
                     QSIFPGRSSRIFLLGEAAGLGLEQEEVPDPFGASLEHYREIALILRKMVLGLLDSINS
                     DPRFSA"
     gene            9103..10197
                     /locus_tag="P5541_01390"
     CDS             9103..10197
                     /locus_tag="P5541_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRV70657.1"
                     /translation="MREKIRKIARALLAAIILLLSVPFLSAASADDIEKEIKLGEKVS
                     KQIEEHWERVVDPVRVARLSMILGRQLPFTERDLPFEVRLIEEKRPNAFALPGGIIYF
                     TTGMLDFCRSDDEIAAVMGHELTHADKRHVMIQVARNQRLSIGALAVLVATRGQGVAP
                     ILANLAQVAIMNSYSRDLEREADQGGLEFLNRSGYEPSAAVTVMERLLEEEIKRPYVD
                     PGIYAGHPRTRERISELVKMMKTMGLPVRRKRSLHLLRTEITESDERMVLAIDGTPVW
                     WGVSSEGTEELLKKANEAILVHLQMETSAFDVMIAGVAPERILKIGLGTVARESEIQE
                     DMPAIEDFRENILDSLQKARAIHPVADYNR"
     gene            10224..11963
                     /gene="dnaX"
                     /locus_tag="P5541_01395"
     CDS             10224..11963
                     /gene="dnaX"
                     /locus_tag="P5541_01395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRV70658.1"
                     /translation="MHVTLYRRYRPGCFSEVVSQDSAVDLLRGEISRGRHGHAYLFSG
                     PRGCGKTSLARIVASALNCTRRRDDGEPCRECESCVSISSGNSLDVVEIDGASNRGID
                     EIRELKEHISLSPFSGKYKVYIIDEVHMLTEQAFNALLKTLEEPPETVVFVLATTEPH
                     KVPSTIRSRCQHIPFHRIPAGSIVVSLERVSMEEGVKLEGDALWEIARESDGSLRDAL
                     SLLEQAIGTGLTEISLKDIRGILGGGGRSELEKLAVQLRKGTAEALLLLEEMLERGLN
                     AERFLEGIFILFRDLLVASRWKDEGISALPLSEGEKAFLREESPFWKQEDLWKILEFC
                     TRNLPRARFGLKTEIAFGMFLGLFPEGDKTGKKPGKNANIPLQDTSPSFQIKDETDVM
                     STQSLIASGTGRAEADVTPKGSPEQWDELLECFREENILLYSACVVATGISKEEVYSI
                     IFPEENRFAFEMASSPRCLACLESIRINKFPHLKMDLLWGEKKVEVETVPDEKVDEKH
                     DAKAVNEEALPRKRKARGHGETSSEPQTETVSQNGSNGLGDIQSFFNAELLILREENQ
                     DLPEAQEGETEEQ"
     gene            11960..15373
                     /locus_tag="P5541_01400"
     CDS             11960..15373
                     /locus_tag="P5541_01400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRV70659.1"
                     /translation="MNNPFVHLHVHTEYSLLDGAIRCDDLARRASEWECPAVAITDHG
                     ALYGAVEFYVACRNAGINPVIGCELYVDPRGHTLKDRLGRNYHLVVWAENMEGYQNLV
                     RLVSIANTDGFYYKPRVDHDLLARHSKGLIASSACLAGEIPALVLEGDEKGATERAVF
                     YRDLFGEGNFFLELMHNSLREQAVANKALIRISQKTGIPLIATNDAHYPEAEDAGWHD
                     VLLCVQTNSTVNTPNRYRFGADDFYLRSPEEMWKLFGADVPDALTRTLEIAERCNVEF
                     SFGSYRLPVIEVKKGSTLEEELAIKSREGLQDRFRGRDIPPEYSERLEYELGVINQMG
                     FAGYFLIVSDIIQAAKDEGIPVGPGRGSAAGSVVAWSLKITEINPLQYGLLFERFLNP
                     ERISMPDIDTDISDRGRDRVLEIIVEKYGRTHVSQIITFDRMKSRAAIRDVGRALDMK
                     YPDVDRVAKLVPAGATSIGDALEQSADLKQSKQDDPAVSRLLEYASRMEGIARHCSQH
                     AAGVVITPEPITEIVPVRKIGEDQIVTQYAMEPIERLGLVKMDFLGLRTLSMIEEALE
                     NIAGSGKEVPDMYSLPMKDPGTFDMLCRAETLGVFQLESSGMRQLLRQLKPDCFEDLI
                     AVLALYRPGPLGSGMVEQYIDCKHGKRDISWLHEELKDILGETYGMILYQEQVMQCAA
                     RLAGYTLGQADLLRRAMGKKKADVMETQRKQFVAGCLERGVKPAQAEHIFDIIQEFAG
                     YGFNKSHSAAYAMITYQTAYLKAHFPSEFFASFLSSHIHSKLDVLAGHVRAVRDSGIK
                     VVPPDVNRSVSGFSVDGEQIIFGLDAVSKVGHPSVEQIVTVRRDGDFISLWDFVNRVD
                     TRIVGRAVIENLVRAGAFDSICTNRRQLLESIPVMLDIVQRRASENSMQVLLDLGEEK
                     ESVEGPAMPEIDDFDPHERMDMEKEATGLYISGHPFDHYEREVNQFVTCSIQELDFWK
                     GGECPVSVAGIVVGVQERYTKKGDRMGILEIEDSCGKIEVICFPRMWTSLACPPEKGC
                     LVIVNGTPRNREGLSIIAESIIPRESIQDQARSWIRLRIDMNGTGTGVNMREVLRELK
                     GCPGDAGVILDVALDGKRAILRSRDLKVRPDDGLARKIFEISGGGVEVV"
     gene            15419..15679
                     /gene="mtrB"
                     /locus_tag="P5541_01405"
     CDS             15419..15679
                     /gene="mtrB"
                     /locus_tag="P5541_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HRV70660.1"
                     /translation="MNGDECSDRSVLSDFIVVKAHENGVSVIGLTRGHDTKFAHSEKL
                     DAGEVWIAQFTEFTSAMKIRGKATILTAHGAVESGRPDNETG"
     gene            15666..17435
                     /gene="pyk"
                     /locus_tag="P5541_01410"
     CDS             15666..17435
                     /gene="pyk"
                     /locus_tag="P5541_01410"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRV70661.1"
                     /translation="MKRAKIVCTLGPACLEYGVLKKMIQAGMNVARLNFSHGDHESHG
                     AHLDNVRLAEKELRVSVGTMIDTRGPEIRTGRLEGHVPVTLVPGGVLILSAGWEGDGN
                     TEKVGINYPDLAREISPGDSLFVDDGTLHLVAESVSGTNVVCRVAVGGELGENKGVSV
                     PGVRTSLPVLSRRDIEDVRWALERKMDFIALSFVRDRADIMEARKVIEESGGSMRIIA
                     KIETREAVDNLEDVIEVADGMMVARGDLGVEMPLEDVPLVQKRIIDLCRYQGKPVIVA
                     TQMLDSMIRNVRATRAEASDVANAVLDGADALMLSGETANGAHPVLTVETMARIIART
                     EREESPWSRRLPEGKVEGIPDAVSKAAAMISDRMQARAVISLTRSGGTAQMVSKYRPG
                     APIIGATPEESTMRSLSMVWGVTPLLVPMGKDLETALDSATSIALKESLLDEGDLVVA
                     TAGFPIGSPGTTNSIQVMAVAKTLLRGLSLQKRDAWGVVVKAFSSSEAGEKMSDGAIL
                     VVRQTDRDYVPAMKKAAAVICEEGGLTSHAAIVSLELRIPCLVSAKGALSALDDGMTV
                     TVDGRRGVVYKGVVKLHPDENDQ"
     gene            17425..18039
                     /locus_tag="P5541_01415"
     CDS             17425..18039
                     /locus_tag="P5541_01415"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRV70662.1"
                     /translation="MTSNGLTDVERLFVSIPFEGHARSWKEIPGNPEKQSAVLVPVLA
                     EGECFSLMLVERSKLLRRHPGQISFPGGARDEDDHGPVDTALREFEEETGISGSKVEV
                     IGNLTEEYAYSSDFILYPVVGFLGRSFSAEDIRPDPVEVERIFTVPLAFFFGVPKMEE
                     FFRNGMTFGYPVYTFKGLRIWGATAWIIRRLVIAMGKDPGPGCL"
     gene            18039..18881
                     /locus_tag="P5541_01420"
     CDS             18039..18881
                     /locus_tag="P5541_01420"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRV70663.1"
                     /translation="MSLVGTHVSAAGGVWQAIERGEALGCESIQIFTRNQLQWRARPL
                     SESACDRFMRAWRSSRIKKVVAHAPYLFNLAAEGDTRKRSIESLAGEIQRCDQLGIDD
                     LVLHPGSHLGLGEEAGRKLVSDGLKQVIALTDSSRTRVLLETMSGQGNTLGSTLDELA
                     WIFDEVDCFPRLGICLDTCHLFAGGYELRGRVSYERLCSSVERLFGLDNIRCWHLNDS
                     KKGLGSRLDRHQHIGEGMIGSELFSCIVSDPRWNNTPCLLETPKDGPGDEGNLALLRK
                     MRGF"
     gene            18921..19994
                     /locus_tag="P5541_01425"
     CDS             18921..19994
                     /locus_tag="P5541_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRV70664.1"
                     /translation="MQYPEISVRTNLIAENAKKIAELCAPSGIKVWGVTKGVSAQPSV
                     AAAMIDGGCCGLADSRIENIRKLRLFGIREPMMLLRIPMPGEMDHIAEFADICLVSMP
                     ESVLMLEEACQRAGRDMGVIVMVDLGDLREGIWPDETAPMAEALRRCRKVRCKGAGSN
                     LGCLSGVLPTPVNLSLLVDVTRELGSFLGYPMEYVSGGATSSLSLVENGTMPSGVNQL
                     RVGEAILLGTDVTRDRVIPWLHRDAITFSAEVVEVRSKPSVPIGETGSDAFGNKPVFE
                     DRGTRRRVIVAAGRQDVRPEGLVPISPGITVIGASSDHLTLDAGDLDGEVSPGDVINF
                     RPDYGAVLAATTSPYVRVKISLS"
     gene            20021..20857
                     /gene="cdaA"
                     /locus_tag="P5541_01430"
     CDS             20021..20857
                     /gene="cdaA"
                     /locus_tag="P5541_01430"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRV70665.1"
                     /translation="MIGPIRWQDFIDIAVIAFIVYRLLLLLVDTRAMQLVKGLLVIGL
                     LFALARWVHLAALSWILGRLLGVFIIAIPIVFQPEIRRMLEELGRGNIWRRRQAEEKA
                     AQLTDEITRALLFLQNKNIGAIIVFQRSTGLRDIWRSAVQIGGDISQDLILSIFWPGN
                     PLHDGALILDREKVIAAACYLPLTENSSLSRWMGTRHRAALGVTEVSDAMSLIVSEER
                     GEIALAINGHLSRELKEAQIRRLLAHYFSEAPDSGSAIEGVKGRFAALFSGGTGKNDQ
                     VD"
     gene            20841..22055
                     /locus_tag="P5541_01435"
     CDS             20841..22055
                     /locus_tag="P5541_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRV70666.1"
                     /translation="MIKWIDRLLSSRLFLKGISLLVAIFVWYYITADRGTEIVRTVTV
                     PLEYLNVPSDMSVSSSVRDVDVQISGARESVFSLGASVASQVDLLGLEPGLHRRPVQT
                     LLPSGARLVEVSPQNVEIELTRMGSRLMPVMFEPPPDLPPGYRVEDVSVMPGEVLVKG
                     PEESISLLENVVVLPTAGQILEEKNLTLPISLSPGQEMDRDQFRVDPSQALLSFRLVK
                     GFPRKIVPVRLEMTGVPSRDYQIEAVAVDPPEVTIQGPLESVERIDELFLPPIDVTGI
                     SRDFSAVIRLGDPSDDVEIVGEKNVNIRIMLSGKVENRLYARIPVRVSGKSIYPGWRV
                     EPSEVSVYLEGSPSDLDAAETRGVPVEAFVEVTNLVSTRIRVPVQYEVRGKGLKLSRV
                     EPSTVMVYALEE"
     gene            22085..23491
                     /locus_tag="P5541_01440"
     CDS             22085..23491
                     /locus_tag="P5541_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRV70667.1"
                     /translation="MPESGEKSRCLFGTDGVRDVANRGIMTPEMALRLGRSYVLFLVE
                     RGISRPSIAVGRDTRRSGPMIECALLSGLASAGADVTSLEVMPTPGVSFAVTRLSCDG
                     GAVVSASHNPSEYNGIKFLGPDGCKLNDSEEAAIEDYLGDILFDEWRPTGPSVGTVSY
                     GRELSSDYSRWVLDTCCIDVDPDLDIVVDCSNGAASYIAGELFGKCFRRARVIENIPD
                     GSNINENCGVMHMNSLASIVTSGGNSLGMALDGDADRVLFVDARGRLIDGDIMLWVIG
                     RWLSKEKKIGKGVVATVMSNKALEERLGSEGIPVTRCPVGDRYVLDAMRRSGSLLGGE
                     QSGHMIAGDFVATGDGLCSGLLFLTACRVLGEDTSTLVDRFGRYPQILRNFHSADKDE
                     FMRRPPIEEAVERAVRDLGDMGRVLVRPSGTEPLVRVLVEARDEATLRSVSGPLFDTI
                     AGEENLDRQDGSLEVTPR"
     gene            23488..24399
                     /gene="galU"
                     /locus_tag="P5541_01445"
     CDS             23488..24399
                     /gene="galU"
                     /locus_tag="P5541_01445"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRV70668.1"
                     /translation="MKGNIRKCLFPVAGMGTRFLPATKEIPKEMLPLIDRPLIHYGVE
                     EAVSSGCSDVVFVTGRGKRAIEDYFDRSWELEHALVEKGKTEQAAMIRMISDMANFSY
                     VRQPEPLGLGHAVLCGESFCGNDYFGVILPDDVMVSTPGEDPVLGQLIKIHNRFGGSV
                     IALEEVPFEETYRYGIIEGETEEDRVYRVRDLVEKPEKGKAPGRLAVMGRYVLSPGIF
                     DHLRSVPRGAGGEIQLTDAIRALLKEETVRGVLYRGRRFDCGTMESWLRATVSMSLQS
                     GDLAEVILKEVEQFRPCENRLSGSERY"
     gene            24592..26424
                     /gene="glmS"
                     /locus_tag="P5541_01450"
     CDS             24592..26424
                     /gene="glmS"
                     /locus_tag="P5541_01450"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRV70669.1"
                     /translation="MCGIVGYVGKRNASDVLVDGLKRLEYRGYDSAGLALRTEEGILI
                     LKEVGKVADLEALLGESSPVSTQGIGHTRWATHGGVTQANAHPHRDGSGKFAIVHNGI
                     IENFMELKEELEMEGVTFLSETDTEAVVHMIARTYSDKGDMLATLVALQKKLRGSFAL
                     VVIQQDFPDRIYCVRRGSPLVVGSGEGESFCASDVPAFLPFTRKVQYLEEGDIAEISL
                     DGIKIWNEDGVLQNKPFLTVDWDVSMTEKSGFSHYMLKEIHEQGMVLRSTLKGRIQEG
                     RTSIEHELGWDPEMVARWNRIHVIACGTSFYAAQVAERVFEKWTDFDIKVDIASEYRY
                     RHIRCDSSTLAIFVSQSGETADTIAAQRKARAEGAHCLAITNVRGSTLAREVHSVLLL
                     KAGPEIGVAATKTFSGQMAALFLVALQLGRMRNSLCPEEEERLLNEFLQIPYKVEILL
                     EREQEIKELAEKYAASENFLFIGRGISFPVALEGALKLKEISYVHAEAYAAGEMKHGP
                     IALLEPAVPVLSVIPRDGLYEKTLSNIQEAKARRSPLIAVCTEGDQLISKHVDDCVYV
                     PWTDEEFSPFLTVVPLQFLAFHIALLKGREIDQPRNLAKSVTVE"
     gene            complement(26453..27511)
                     /locus_tag="P5541_01455"
     CDS             complement(26453..27511)
                     /locus_tag="P5541_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70670.1"
                     /translation="MKRFSLFLVLFVLLLQSPAFPAIDGPFTIALKCVPGGDITILRP
                     GEPPQTAGRVVALPDGSRWPSFTASAWGHPGTVTATAVNALHILLSVEKGRGRTMSIL
                     PAETIAPAAGPGSAIVTDIRAGRSIFGAWAPSSGSAVLIIPSGKTTPETARLPLEGDT
                     LLIRVPSSDNPYYLEIENRPGGRLTAWYDSGPKVEARVIRPLGGSGRFDGTLYQSAGR
                     IRANHPGVIDICTSPEGLIGGFQIMPLEHASSSEMQGAWKMTQWMIVASADAGAQTLK
                     GTVPLFSGGLVPGTATGEKLWDLWATYGRKPLVLVRIRGGNWGQMPKVSGREDHAFRD
                     ITHLRVYYPFTEEPQAGY"
     gene            complement(27508..27759)
                     /locus_tag="P5541_01460"
     CDS             complement(27508..27759)
                     /locus_tag="P5541_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70671.1"
                     /translation="MRWISLSAVISFFVMSAIFGVLAARSGEKRQVLLFWLFLALFIL
                     ASFLSLLSDAPLWVAVAPLVPAPIFIMILFSAGKWRNGS"
     gene            complement(27756..28220)
                     /locus_tag="P5541_01465"
     CDS             complement(27756..28220)
                     /locus_tag="P5541_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRV70672.1"
                     /translation="MWTIEEGTEKIRSKGLKITSQRVGILKVLKGRKDHPSAEQVFRE
                     MKKDHPTISFATIYSTAQILEDAGLLQILTIDGHRVFFDPNPEPHAHYRCRKCGHVFD
                     IPLPSGTLKKITEASGEIVESIQVYCYGTCSACISREREKDREPVGNSKNWQ"
     gene            28377..28451
                     /locus_tag="P5541_01470"
     tRNA            28377..28451
                     /locus_tag="P5541_01470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28410..28412,aa:Gln,seq:ttg)
     gene            28600..29994
                     /gene="glmU"
                     /locus_tag="P5541_01475"
     CDS             28600..29994
                     /gene="glmU"
                     /locus_tag="P5541_01475"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRV70673.1"
                     /translation="MARSGTGVLILAAGKGTRMKSDTPKVLLPLLEEPLLYYPLVSTD
                     SPDFSGRGVVVGHGSDKVSAYLEKSWPGSEVIEQKQQLGTGHAVAAARKWISRFDHVL
                     IIPGDVPLLGRETLSMLHSRHLEGDEDCSFLVFEPDDPSGYGRVIHTEKGFRIVEEKD
                     AMEHEKKCGRVNGGIYAFRTTVLLEHIDSLGKDNAQGEYYLTDVIHDLCQEGKKIRML
                     LCPDPEEIEGVNDPQQLDVMSRRLRKRILAKHMSCGVKCVDSESVWVGPGVRLGEDVM
                     LDPFVQLWGDTSLGKGSRIGSFSIVRNSIIGENVHLLGHVVLNDSEIQNDARIGPFSF
                     IREGSVLREGAYVGKFVEVKKSVIGKRSKVPHLSYVGDATLGEDVNIGAGTITCNYDG
                     VKKNPSVIGDGCFVGSDTMLVAPVTLGEGAYTAAGSVITRDVPPGSLAIGRARQKNIE
                     GWSARRKGPKEEGE"
     gene            29997..30965
                     /locus_tag="P5541_01480"
     CDS             29997..30965
                     /locus_tag="P5541_01480"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRV70674.1"
                     /translation="MASKLRELKIFSGTAHPQFTRSVCENLGVGVATASLFRFSDGEI
                     GVSVEESVRGADVYVVQPTCDPVNENLMELLVMIDVMRRASAYRVNVVTPYFGYARQD
                     RKTKAREPITAKLVANLIEKAGADRVIAADLHAGQIQGFFDIPVDHLTGIPLLAAYFR
                     DIFKNDLEKDKIVVISPDIGGVVRARRFAVHLNADLAIVDKRRSHEVANFSEVMEIIG
                     DVDGKIAILVDDIIDTAGTMVQAAEALISRGARKVYACATHGVLSGPAIDRIAGSPID
                     ELVLTNTVPVPEEKNIDKITTLSIAPLFAEAISRIHSDHSVSILFR"
     gene            31015..31671
                     /locus_tag="P5541_01485"
     CDS             31015..31671
                     /locus_tag="P5541_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRV70675.1"
                     /translation="MDTAERIKLEKRETSGKQACRKIRNEGKIPGVLYGPGIDGAVPF
                     QVSSARISPLANSGRWETARLEVDVPGGKKASCLMRDLQRDPLTGKILHVDLFQLKKG
                     HKIMVNVPVEIAGREKCIGVKQGGVFEQMIHEIQMEVLPKEIPDSIVVDISSLKIDGS
                     VRISDLEMPGSAVLDLSEETIVVAIGHARAEAEKTDEEEEEETEVQVVSKGKAKEEEA
                     "
     gene            31668..32285
                     /gene="pth"
                     /locus_tag="P5541_01490"
     CDS             31668..32285
                     /gene="pth"
                     /locus_tag="P5541_01490"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRV70676.1"
                     /translation="MSPIVILVAGLGNPGSRYAWTRHNAGWHVIDLLRERLSAPRWSS
                     TRESEIWGPCKVDDHKVFLLKPMTYMNLSGVAVKRSVRSLGIDLEDIIVVYDDIALPF
                     GRIRFRPGGSAGGHRGMISVIGHMGSAEIARIRIGIGGSDPDRELIDYVTDPFTREEL
                     SLLPSICDRASDSVLLWISRGPEETMRVTNAPPDHPRENRSGDRD"
     gene            32331..35462
                     /locus_tag="P5541_01495"
     CDS             32331..35462
                     /locus_tag="P5541_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRV70677.1"
                     /translation="MNGIDRKGSADSLSSISNTVTTGTNTVHIPAGTAAWCWAMRDLE
                     GPALVILPDRRVLAQFVADWEQLVPEKDIQALSEIPLSKELIINDAIRMERGCSTMKW
                     LASGDLMAATAPAVMAPLLLPSERIEIGEGIEAGRDVLLEWALASGYERSDLVWQEGQ
                     FVSRGSIVDLFDPSYRYPLRIEFFDDRVESIRIFDPATQRNISPVGVTKLLSLKRGTR
                     KTFQAMIPPSAKIIFVERRAISRSAEAYKWLWDGIAGNIGIDSLEEWEKVEYGFRSNR
                     SFHLTKAIEGSSYRLDISEIPSFRGKLDAAESFVRGWKNDGFHVTVLSSSDLMLEWAG
                     INGVVGKRGNISGGFCDRTEKKVFLSDSDLTGIVPVISRGKVTRIPPREWEERIEDQD
                     YVVHEDYGIARNLGIERISSGGMDRDCLVLQFAENRRLMMPLVQLFKISPYPLPVGED
                     RELDSLRGKAWKKNAAKARERAKEAARQIISLYAARESASKIPLPPDGEIMRNFEKGF
                     PFIETADQISAIKAVKKDMENSFPMERLLVGDVGFGKTEVAFRAAIKAAESGKQTLFL
                     VPTTLLASQHYESFISRITGLPVRSEVLSRFVPKKRQDEVKRDLESGLVDVVFGTHKL
                     LQGDLKVKNLGLIIIDEEHRFGVLHKERLKKIDPSVDVLSISATPIPRTLQMALGGLR
                     SISVISTPPHNRMQVMTYAGPWRDDLFRESVIREIARGGQVFYVHNRIESIEKVASGI
                     RRQFPGVVVDIAHGRMDERALEEAMSRFASRETDLLVCTTIIESGLDLPGANTLVVDN
                     AQDLGLAQMYQLRGRVGRREAQAYALFFYPADVSLPREAMERLEAIAGMDGDEGGFAL
                     ARRDMEIRGGGQIAGTRQHGHAEKVGYHSYFKMLEEEISLASDKKTVPVVKMDIRLPV
                     VLPSTYVPQSSVRIVIFRRLLKVSDIKETEELKNELRERFGPLPQSVEFMLASAVIRT
                     LGAYHGLQYVRCSLKETCAAVDVDHPSRLPRRAGWMQKANGTIVGPGGYRGMLELAGM
                     IEVSEEVKTEARQ"
     gene            35459..36301
                     /gene="mazG"
                     /locus_tag="P5541_01500"
     CDS             35459..36301
                     /gene="mazG"
                     /locus_tag="P5541_01500"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRV70678.1"
                     /translation="MNDHEKVSLRIKELLVIMEKLRSPGGCPWDREQTMHSLRPCILE
                     EAYELVEAIERDDDPSVSEECGDLLLQVVFISCLAAEENRFGLAEVIEGLNSKLVRRH
                     PHVFGEQKIDTSDAVLRNWERIKQKERKARNNDTSVFAGVPRNLPALARARRIQERAA
                     KKGFDWLPEETEPVISKIREEIEELSGAVASGNGDSIEEEMGDLLFAMVNLSRRLGYE
                     AEFSLQRANRKFMDRFRFIEESVESSDRNWSDYSLEELEDLWQKAKKNLNLDSSVDAT
                     HCRR"
     gene            36363..37781
                     /locus_tag="P5541_01505"
     CDS             36363..37781
                     /locus_tag="P5541_01505"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HRV70679.1"
                     /translation="MVEKTKNSPGRKIGITETALRDAHQSIMATRLRTEDMIPVAEEM
                     DDAGYHSLEVWGGATFDTCMRFLNENPWERLRTLRKCFKKTRLQMLLRGQNVVGYRHY
                     ADDVVREFVKRAVGNGLDIIRVFDALNDLRNMEIAADQVKKEGSSLQMCISYTISPEH
                     TLKNFADLALKMKGMGADSICIKDMAGLLGPMDAFKLVGAIKEKTSLPVQVHSHYTSG
                     MAAMAYYASIQAGADVVDCSISPLAMGTSQPATETMVAALSGGEFDTGIKLEKLIPVS
                     EHFRRVREKYSSLVMGLEGVDINVLIYQIPGGMYSNLVSQLNEQNSLDKLQDVLREVP
                     KVRKEMGYPPLVTPTSQLVGTQATLNVLTGERWKIVSKEVRNYFLGYYGTPPGKVDPD
                     TRKKVIGDDKPITCRPGEVLEPELPGVYKSMGAWITQPEDALSYALFPAVAKDFLVSK
                     FARENLRDVGLEPLVDDAGYPL"
     gene            37847..38851
                     /locus_tag="P5541_01510"
     CDS             37847..38851
                     /locus_tag="P5541_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRV70680.1"
                     /translation="MIEPEINRGPIASGVSQNAIEAPGIEVLARLLGPGDENLRLFEE
                     RYPVRIAVKDLSLVVQGPDASVVVMVEELLSQLLGVARRGHVLRPAEVTYAMNVFAEE
                     GVVDLLPMYEDVICMTYRGKPVRPRTSGQKRYVDAIRKNDVLFAIGPAGTGKTYLAVG
                     LAVAALKEGKASRVVLVRPAVEAGESLGFLPGDLREKVEPYLRPLYDAFYEMFSPERF
                     MKYVEKKVIEIAPLAYMRGRTLNDSFIILDEAQNTTPEQMKMFLTRLGFGSKAVVIGD
                     VTQIDLPSGKDSGLKVVGNILRDISGIGFISLDKTDVVRHEVVQRIVQAYEEYEQKKQ
                     "
     gene            38832..40877
                     /locus_tag="P5541_01515"
     CDS             38832..40877
                     /locus_tag="P5541_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRV70681.1"
                     /translation="MSRRSSEGPLVISLAKMIRKLAADPEYRFRWLASIVVVSAAVLM
                     LAASWWLSEGGGSFRQGAPAPRTYFAHSRMKIVDENATRALREQRIAEIVGVVVKDRA
                     HGEEAEGMLVLLERGEYGEALPDSLAVILEALGEDSRNRVISVSVMIAEELIGSPEMA
                     LDDDDPIWQKLSLSPLSVPEQNLAFQILDHILGSSFKEDPQATLKFKQQVASEVVPVE
                     RIIGVGETIAREGQLISPEMAIILRAQGYPEREIPWKTLLFVFLATTLWIFWMWWYTG
                     RRDFSFDRREWLFIIFILITGWLAMFVSSRFAGNVLGIIALAGWAYLGLPGSFSFHLL
                     IWGGVIGSVIASGSSSMDLMLSVFATVLTASLGYVLMTRIDSRWCLIRKLLLLGLGLS
                     FGVYVIMWGLYLPSGWKILVTYVFSAVVWVAVTIGALPLWEKFFDILTPIRLVDLTHP
                     SHPLLKRLQIEAPGTYHHSIMVGTLAEAAAQGLKMNALLVRAGASFHDVGKLKRPQFF
                     VENQQTGHNPHDELSPTLSALIIISHVREGLEIARQYGIPQKIRVFISEHHGTTCLGY
                     FFKKASITEPDLQMDQFCYPGNRPASRESAVVMLADSVEAAVRAAAGDIRNPKELEEV
                     VSNVIETKISESQLDLVPLTLQDITRIKIAFSETLKHMYHTRRVSPVSEETKTEEDA"
     gene            40874..41392
                     /gene="ybeY"
                     /locus_tag="P5541_01520"
     CDS             40874..41392
                     /gene="ybeY"
                     /locus_tag="P5541_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRV70682.1"
                     /translation="MKVHIDYEPGSFSKRLKPELVSGLRKVLDPVTRELVLSVSEENV
                     LPDEARFSIFLVDEEEIRALNLEYLGEDSPTDVLSFPVFDFEHDGVFHSDAPLVLLGD
                     IVICPPQVAANARVAGHSFARELSLVLIHGILHIFGMDHDTEEKREAMWKKQEQYWDK
                     IEEALYPLEWDR"
     gene            41416..42699
                     /locus_tag="P5541_01525"
     CDS             41416..42699
                     /locus_tag="P5541_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRV70683.1"
                     /translation="MSEEIAQSILILAFLLGLSALFSSSETAITSIGRGKLLAIQEKN
                     PQKKRGIEWLISDMHRALTVILIGNNLVNIAASAVATSLALSLFGPKGLLVAVAFMTV
                     MIVVFGEILPKSMAIIRPEKIVTATLPLLRFINVIFAPLIWATVVTVKFLGSLMKVDL
                     SSQHPFVTREEIEQMVNIGEASGVFEEDERRMIHGVISFEETRVYEIMVPRTDMVTVS
                     GEISVQESVSVFRECGHSRLPVYDRDPDHILGILYVKDLIGSLISGETAVPVSSLVRD
                     ALFVPETMRIADVFDVMKGKRVHIAIVVDEYGGTGGLVTLEDLIEEIVGEIQDEYDDE
                     SALIQKEDDGSYLVKGHMGLEDLSEMLHYPFESSDMDSVGGLILSLAGRFPDEGQRIP
                     YGHWEFQVLEVEDHRIKLVKITPMDDMYRICEVVE"
     gene            42696..43121
                     /locus_tag="P5541_01530"
     CDS             42696..43121
                     /locus_tag="P5541_01530"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRV70684.1"
                     /translation="MNQGYDWKAGGVTADMLLEEARRVQRFSYSPYSGFPVGAAVVAA
                     DGTIFCGCNVENGSYGLTICAERNAMTRMVSSGGKDPVAIAITGPPGVPCPPCGACRQ
                     FLAEFNRRLLIVLEAPETGHVILSLEDIFPMPFSFGDRG"
     gene            43124..44032
                     /gene="era"
                     /locus_tag="P5541_01535"
     CDS             43124..44032
                     /gene="era"
                     /locus_tag="P5541_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRV70685.1"
                     /translation="MSDAGSRSGFVSVIGRPNVGKSTLVNAIVGGKAAIVSDKPQTTR
                     NRIRAVYNDPRGQIVFVDTPGLHRPLHELGRFMERQASESLGSSDAVCYVIEASDTHI
                     GKADEKILKIIEMSSVPVVLVVNKTDYDSPARDKKGWKVFLERLSSADVVEVSALKET
                     NMDLLLEKIFSLLVPGPPIFDDDRMVDCTERFLASEIIREQVLMQTEQEVPHSVAVDV
                     EEFQSPEEYPERKNLLVRASIYVERSGQKAILIGKGGTKLKNIGRKARLELQQQFGHD
                     VYLDLWVKVRPGWRNSGNELRRMGYT"
     gene            44044..44757
                     /gene="recO"
                     /locus_tag="P5541_01540"
     CDS             44044..44757
                     /gene="recO"
                     /locus_tag="P5541_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRV70686.1"
                     /translation="MENKSGQGILKNRGVVLRRSDTPEGNRSVYVLLENVGPVWLLAP
                     GAARGKVRFGGSTDPLVWGTFHVYRGPNRWYLRDVDVRKDFWSLRSSSIRIRQAVHWV
                     GSILGNTLPGHPCNDLLPVFFWSLSLLENPSAREDLASWRFYWRWLRIWGMAPELERC
                     RSCGSLPRRAVFSGEGINCEKCGGQGIPLSEQERLALLEAAISGHRDFIERSGQPGIG
                     SRLVEKCTLVLERELAERT"
     gene            44785..45675
                     /gene="glyQ"
                     /locus_tag="P5541_01545"
     CDS             44785..45675
                     /gene="glyQ"
                     /locus_tag="P5541_01545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRV70687.1"
                     /translation="MNLQEIIMRLERYWAEQGCIIQQPYDIEVGAGTMNPATTLRVLG
                     PEPWRVAYVEPSRRPTDGRYGENPNRLQHYYQFQVIIQPAPEEIQEMYLESLKTLGID
                     PGDHDIRFVEDDWESPTIGAWGLGWEVWLDGMEITQFTYFQQVGGIDMDPVAAEITYG
                     IERIAMFVQKVDNVYDLEWVGKVKYGDVHHRGEVEHSTYNFEIADTEMLFRLFSMYET
                     EAKNILEKGLVFPSYDYVLKCSHAFNLLDARNAISVTERTGYISRVRALASGCCQAYA
                     KQRKDMGYPLMNSFDTENGR"
     gene            45680..47746
                     /gene="glyS"
                     /locus_tag="P5541_01550"
     CDS             45680..47746
                     /gene="glyS"
                     /locus_tag="P5541_01550"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRV70688.1"
                     /translation="MGKRDLLLEIGTEEIPSRFIPPSLSELSRIAGSEMKTERLSFDN
                     IQTFGTPRRMAFIVRDLAERQEDLIEEFKGPAWSSAFDGSGNPTKAARGFAKSRGVEV
                     DSLEKRTFGENDFAVAVIRQEGRPVIEILPGLLERITGGISFPKSMYWDRTFTRFARP
                     VRWLLALYGDTPINFGFGSVKSGKTTRGHRFMGKKIIEIENTAEYLEKLHDNFVIADP
                     EKRKEKMVSGIAGLEKEIGGKAVLDEDLMEENLFLVEYPVPFFGSFDQSFLDIPAEVL
                     ITSMKNHQRYFPVRDTSGALKPFFVGVSNNRATNMSVVREGNERVLRARLSDAAFFWK
                     EDQKVALSARVESLKKIIHHEKLGTVYEKVSLVRELCSFVSETLSLSREETELVDRAA
                     MLSRADTATEMVYEFPELRGVMGREYALKGGEDPRVALALYEQYLPAFSGDDVPKDKI
                     GGILGICDRIDTITGGFKAGLQPTGSQDPYGIRRASRTLNEILWGSRIDVDILQILAE
                     SARQRGLSEAETLTAREFILQRLHNQLREKGFSHELTTLAVSVAGSRPMQAMRMLDVF
                     SGIQDSEWFSGLVVSAVRVKNILQKAQERNMNLDSGLLVEKEEKELFEIVETLSPDVA
                     SAVAGSDWDGLARLLARLEPFITSFFDHVLVMDKDEKIRSNRIALLEKCNDLFRTAGD
                     LGVLKS"
     gene            47788..48609
                     /locus_tag="P5541_01555"
     CDS             47788..48609
                     /locus_tag="P5541_01555"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRV70689.1"
                     /translation="MNDPESKAPLPVFVVSDFTGETAEHVAKAAMSQFATDAVTLQRY
                     RYINNQERALEVLEEAGSINALVVCTLVDHDLRRWFMEKAHLQKTDTVDIMGPMLDML
                     EMRLEHPPLETPGLMRRMDEEYFRRVKAIEFAIKCDDGRCSEQLLSADVLILGVSRTS
                     KTPLCMYLAHRGFMVGNVPLIPETETPKEIFDVAPEKVFGLVVQPEKLVEIRRERLRL
                     LGLDSDTSTYAQWERVEEELKFAKDIMRKIGCRILNVTNRAIEETAQEILDYLRA"
     gene            48645..51305
                     /gene="ppdK"
                     /locus_tag="P5541_01560"
     CDS             48645..51305
                     /gene="ppdK"
                     /locus_tag="P5541_01560"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRV70690.1"
                     /translation="MTEAGKTEKRFVFNFSEGRADQKKLLGGKGANLAEMMRIGLPVP
                     PGFTISTEACLEYISKGEPFLDGLWEHVLSYINGLEKETGKTFGGEKSPLLVSVRSGA
                     AISMPGMMDTILNLGLNETTVKALAAESGDERFALDSYRRFIQMFSDVVLGIDGDLFE
                     NVIHSAKKDLGLSYDYEIPASSWKDIIGKFLRIVEQESRGPFPSEPMVQLRKSIEAVF
                     RSWDNPRAKTYRKINKIPDDLGTAVNIMTMVFGNLGNDCGTGVCFTRNPSNGEKKLYG
                     EFLINAQGEDVVAGTRTPQPIDFLGEKLPEASSELHRVSELLEKHYKDVQDIEFTVER
                     GKLFMLQTRNAKRTSRAAVQVAVDMHAEGLIDATTAVSRVTPDQVEQLLHKQIDPEAS
                     FDIAAKGLPASPGAATGIVIFDADEAEKVSNSGQPVILVRPETTPDDIHGLYAAQGVL
                     TSRGGMTSHAAVVARGLGKPCVSGCESAVIDVNRGEMQIGGKTVRKGETITIDGTRGH
                     VITGVVPLVQPSFTDDFRQLLDMADSISDLQVWANSDTPEDSERARSFGARGIGLCRT
                     EHMFMATERLPVMQEMILASDKDSRVEALEKLKIMQRDDFRGIFEAMEGLPVTVRLLD
                     PPLHEFLPKEEELKETIASLKGQSGKELEKAEAILKRVLSLKENNPMLGFRGCRLGLV
                     YPEIYEMQVCAIIEAACELSLAGKKVIPDIMVPLVGTKEEMKRLDRMVRDTAEQVIKN
                     KGLEKLHYLVGTMIEVPRAALVAGEIAEFAEFFSFGTNDLTQTTFGYSRDDAEGKFLT
                     RYVEDGVLKDNPFHVLDRDGVGRLMKIAAKEGREARPGIQIGICGEHGGNPESISFCQ
                     GAGLNYVSCSPFRVPVARLSAAHAKLGLLD"
     gene            51356..52402
                     /locus_tag="P5541_01565"
     CDS             51356..52402
                     /locus_tag="P5541_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRV70691.1"
                     /translation="MTAEIPRIRWEKLERTILAPFAAFSDSSGGRARTEIPCPIRTLY
                     QQDRDRIIHCKAFRRLKHKTQVLLLPEGDHYRTRLTHTLEVCQIARTISRALALNEDL
                     TDAIALGHDLGHTPFGHMGERALDKIAREKGLDGFAHASQSLRVVDHLEKEGKGLNLT
                     LEVRDGILKHSKGQVDVREGFTGGETTLTLEARVVRVSDSIAYINHDLDDAMRAGLVK
                     ESDPPADAIGVLGKTHGDRIGMMVKDTVSNSGPGGVTMSPEILEAVEKMRSFLYSNVY
                     ASERVLREEPKVEHVLRTLFDHYEKNPVGTIAPDGAPPCQMALDHVSGMTDRYALSLF
                     EKIAMPDPWPQIGR"
     gene            complement(52399..54762)
                     /locus_tag="P5541_01570"
     CDS             complement(52399..54762)
                     /locus_tag="P5541_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRV70692.1"
                     /translation="MKTGFSKFFGKNNRNKTDHPETVETGGKKPKGNNVRGALLAVLV
                     VFMAAIALASVVGAIVIRNAASSLPSLEEMSNYEPDLSTIVYDRNQKVITRLFLENRT
                     WVPLSQISRNMQRSAVAAEDSSFYSHGGLDFSGILRAFWVNVTNLRTKQGASTITQQL
                     ARNLFLSREQTLTRKIKEAIISLRLERIYSKDQILEMYLNMIYFGHGAWGIHSASQLY
                     FNKEPSELTLSETSLLAGLVKAPEYYTPIRHPDRAASRQTYVLSRMVQLGMITPEEAQ
                     DAQKIELDFHSLKKPALMFDEAPYFISHILFEHLLPTYGSDVVYRGGLRVHTTIDLDV
                     QHAAEKAIQGLKSEGAIVALDPESGEILAMVGGKDFEVSKFNRATQAFRQPGSSFKPF
                     VYTAALMRGLRPVDHALDAPVSYDNGIDDQGKRNIWEPGNYTREFRGEVTLLTALVHS
                     INTVAVRVAHITGVENILDVSRKAGFTSPHLPADLSLALGSASVTPLEMAAAYSIFAN
                     DGSKVTPFAIRRVVTGAGEILEEYGPQLTKTIPEEIAVSVRSVLMEVVRSGTGTRARI
                     PGYEVFGKTGTTNEYSDAWFAGGIPGLVTVVYAGNDDHKSLDRPGTGGVIAAPVWQAF
                     MSEAVKYLPVRESFTLPESANVQIVTVCKDTGFLVSGGCTASVIILPDGMAPSSICPL
                     HGGSWAMAASDPNVPRMLLSPRDEEVTGITASMARLYGVSISDTAYYGPMDEISSQQP
                     ATTYAPPVSKPGQADTQPEPYVKKERTPEEIEERYQDLLKQYGIEED"
BASE COUNT        13517 a        12931 c        16007 g        12595 t
ORIGIN      
        1 ggtgattcat ggctttcaat ccttgcggtt gagccatggc tggcgacata tcgccttgac
       61 aaaaaccggg agataacgta ttgttcacta acggcggcca gagccgtcga caatcacttt
      121 ctggtaacag aagaaatggg aggcaggatt ttgacaacac agggtacagt gaaatggttc
      181 aacgacgcta aggggtatgg tttcatcacc accgacgatg gcaaggatgt tttcgtgcat
      241 ttcagcgcta tcgagggcga cggcttcaag agccttgccg aaggccaggc agtatccttc
      301 gagatcaccg aaggatccaa gggccctcag gcagcaaacg tccagaagat ctagcagact
      361 gactacttcc aaacactgaa gaaaaaagcg gcgcctttga gggcgccgct tttattatct
      421 tcttttgccg gatctttagg gggagccgaa tggctccccc tctattgtcg attaccctac
      481 ttgatctcgg gaactgtcat gccgacctcg gcgtagtact ttgcagcgcc ggggtgaacg
      541 gggacggaaa taccgttgag ggcagtgtca agagtgatca tcttggcttt ggcatgaacc
      601 ttgtgaacgt cttcgatatt ctcccagaag gcctttgtga tcttgtagac gacgtcatca
      661 gagagattcc cgtcgcatac ccacatggcc tgtactgcta cagtctggat gtccttgtcg
      721 attcccctgt aggttcctgc ggggacaatg tccttcacga agaaggggaa ctgggcactg
      781 agatcctcga tgagcttgtc atcgaactcg atgagggtga tgtcatgcat tgtggccaga
      841 tccattatgg aagctgtcgg gaaacccgca acgacgaatc cgacatcaag ctggccgtct
      901 ttcatcctct gggtggtgtt gttgaaatca aggaaatcgg cgttgacatc agaatatttg
      961 taaccgagga ccttgaagat gagggatacg tcaacctgga ctcccgaacc tggtgctcca
     1021 acggagaccc tcttgcccgg gaggtcgcgc atggaggcaa cgccggattt cttcatggcg
     1081 attgcctgca cggtttccgg atagagagaa gcgatggcgc gcacgttctt gtagggttcc
     1141 ttgaacatgt acttgccctc gtaggcaaat gcggcgacat cgttctggac gaggcccatc
     1201 tcgatctggt tggtgccgat cagattgagg ttagcctgcg aagcgttgcc tgtctcagcg
     1261 gtcatctgga cgtcggaaag gttcttgctg accacatccg cgattccgcc gcctatcgga
     1321 tagtaggttc cgcctgtacc gcctgttgca agcgaaacga acgtctgtgc cgaggcacca
     1381 gccgcaagag ccattaccat aaccagggcg agcgcaaaaa tctttttcat gaaactaccg
     1441 cctcctcttc tgtactcgag atattaggaa tgatacgccg atatctggaa cacgaaaaac
     1501 tcgggcacca cctccctgta gccgatgctt gcaatgaatc cgttcataaa gatcaaacat
     1561 tattgtactg gcaaacaggt ctcaattcaa gttccggagt ctattaaaga ctataagggg
     1621 tttatattgt gtccttttgt cagtacaatc cttgctccga tagtatatac tataatcagg
     1681 ataaaacatt tggcaaaggg tatctgccgg aaaggcaagg gtgataatat agaaatgagg
     1741 ctgaaggact ttctcggaag cgaaaaaaag agcggggctg agatatcccg ggacaggttg
     1801 gaggaggccc cccctgtggc agagcgcttt aatccggatg aaaaggacat gaagagcaaa
     1861 atgaacctcc aggtcagcat tactctctcc gacgacagcc agatcggacg ttctgcgctt
     1921 cttcttgctt cgacgacggg tccaggggag gaaaaacgcc tcaaggacca gatagtggat
     1981 ttcggatgga aggcagtagc gaccgaggtc ggtggcctgg ccggtgaact gccccagaag
     2041 ataacccggg ccgtagtggg ggcggctctc aacggcgaaa tagtcagaaa gacctcaagt
     2101 gagatgcacg gacttatgca tgccgccttt gaggcgatga acggcttcat gtcgatagga
     2161 atgctggaag ccagcattgg cgtaaagatc ggtatcgtcc gtaacaggac ctggatagca
     2221 gttgctgtgg tcggagatac ggcctaccac gcagtcgctc accatgagcg atgtggcctt
     2281 ggagttatgc atatctgaat ccgccaggcg gtcacaggtg gacattgtgg gaggttcctt
     2341 tatttttctc cctccctgtg ctatatttct tccttgtgag attaaccaaa gaacccctat
     2401 ggaggtgccc tgaattgaaa aaggatatac atccggaata cggcaagtgt aaggtttcct
     2461 gtgcctgtgg gaactctttc gagacgagat ccaccgtcga ggagatcaag gtggctgtct
     2521 gttcctcctg tcacccggtt tacaccggca agagaggcac aagggttagc gaggcaggaa
     2581 gggtagaaaa gttccagaaa aaatatgccg ggactcgttt tggcgctccg gcggaggaag
     2641 agcagaaggg ttgattcctg ctgcccgata aaaacatgcc gattagcgtt ttgcttgtag
     2701 ccagcacccc tgaaccggac atgaccgttg ctgctgcagc caggctctgc tacagcgacg
     2761 cttcggccgg agatcttctg gaaaaaatga gaggcgagga tatttcccgg ttcatcggac
     2821 atctccgggg gtcggggcat ttctcgcctt ttgaacatgc cgttttcacc tttgcaatag
     2881 aaggcgtgag ccgggtttgt acccatcagc ttgtcagaca caggatggca agttactccc
     2941 agcagagcca gcgttacgtg gacatgaagg aacctgaagt cgtgatcccc ccctcgatta
     3001 tttccgatcc gactgccctc gatatcttcg agagcagtgt caggcagtcc cataccgcct
     3061 acgcggccat ggtagaggtc ggtattccca gagaagatgc ccggttcgtc cttcctcacg
     3121 ggtggggaac gaagatagtt gtgacgatga acgccaggga actgcatcac tttttctcaa
     3181 tgagactttg ccggagagca caatgggaga taagggacct ggccagagag atgctccggc
     3241 ttgcaaggga gaaggctccc aatctttttg ccgtggcggg tcccgattgc gtgaccaggg
     3301 gaagatgctt cgaaagccgg ccatgcgggc aacccttcgc aaacgttgaa gaggttcttt
     3361 ccgaatgaaa cgtctgtcca ccgttttttt gcgtcttttt tcgtttgtcc tttctcccgc
     3421 gctgctttcg agggaggtcc ctgtaggggg gcaggccgtg atcgaagggg tcctcatgag
     3481 ggggccctac agatgggctc tcgctgtaag gtcacccgat ggaggcatct ggaacaggtg
     3541 ctggggttcc cgtccgtgga ccagttcccg cccctggaat tttcccgttc tcagaggggt
     3601 agcaacaatg gcagagatgc tggtgacggg ttttcgcgcg atatcactct cggcccaggt
     3661 ctccctggga gaagaggaga agattacgcc cctggagctt gcggcgacct ttgcggtagc
     3721 aataatcggg gtggtcggtc tttttattat tctgccggtc tggatatccg accttggagt
     3781 ggcctggctt ggaacaggat ccgcaggtgc acacatcctg gagggaacag ccagagcagc
     3841 ggttttcata ggctacattg cccttattgg tttctggaag gacattgcga gagtttttgg
     3901 ctaccatggc gccgaacaca agaccataaa cgcctgggaa tctgaagcaa gccttgatcc
     3961 ccagacggtc tcaaagtatt cgaggatcca cgcaaggtgc ggcacctcgt ttctgctcgt
     4021 ggtagttgcc gtcagcgtgg ttgtttttgc cgctgccgga catggttcgg tcctgtggcg
     4081 catatcatcc aggataatcc ttttgcctct cgttatcggg atatcctacg agataatcaa
     4141 atggtgtgcc ggaaacagaa gcctgggcgg actgctcatg gccccggcgc tgtggctgca
     4201 gtacctcacg acaagagagc cagatgaggg gcagatcgaa atagccattt cagcgctgaa
     4261 ccttgctacc ggtgatggaa cctgtgctga aagtatcggc ggtggtgaat agaagatgga
     4321 taaagatctg gcaaaaaaac tggaaagtct tgaaaagatg atggaggaaa tcgaatcccg
     4381 catgtcggat ccgtcggttg cctccaacca gggagagaga cagaaacttg gcaggaagca
     4441 tgccgagttg tcaggtgttg tagaggttta ccgggaattt cgtcggctta gcgaagatat
     4501 ggccggagca aggggtcttc tccattcggg tgatcacgag atgacggaac ttgcccgtga
     4561 agagatcaat gatcttgagc cgaaacttga ggaactcgaa tcacagctca ggatactcct
     4621 cctgccaagg gatcctaacg acgacaagag cgtgattgtg gagatacgcg cggggactgg
     4681 cggggacgag gccgcgctct tcgcggggga cctttctcgc atgtacggtc tttttgccga
     4741 gaacaatgga tggaagacag aactcctgac cagcagcgag acaggaatag gcggattcag
     4801 ggaagtagtt ttcagggttg acgctcatgg agcttacagt cagctcaagt tcgagagcgg
     4861 tgttcacagg gtccagcggg tcccggtcac cgagtcaagc gggaggatcc atacatccac
     4921 agcgacggtg gcagtccttc cggaggccga ggatgtagat gtcgaaatca ggcaggagga
     4981 tctcaagata gatacatacc gcgcgagcgg agcaggtggc cagcatgtca acatgaccga
     5041 ttccgctgtc agaataacac accttcccac gggtctcgtt gtaacgtgtc aggatgaaag
     5101 atcccagatc aagaacaggg ccaaagcgat gaatttcctg agggcaaagc tttacgacct
     5161 ggaacttcgg gaacagcagg caaagacagc ggccgaaaga aagggacagg tcggcagtgg
     5221 agacaggtcc gagaggataa ggacttataa ttttccccag aacagggtga cagaccaccg
     5281 gataggtctc accctctaca ggcttgagtc gatgatggaa ggcgacttgc aggagatgat
     5341 atcagccctt gtcatggcgg accaggccga aaaaatgaaa gcggcggagg gttaagcctg
     5401 gatattgagg aggcccgctc tctggccgca agggaacttc ggtcagccgg tgtggacaga
     5461 ccctggtacg aggctgacct tctggtcggt cacctgacgg gccttgacag agccctactt
     5521 catacaaggc cacattttcc cttcccttca acccttgttc ccgggcttct tgaagccctt
     5581 tccaggagga aggccagaga accgctccag tatattacgg gcgagtgtga tttcatggac
     5641 atgaatctgt ccgtgggtcc tggttgtctt gtccccaggc cggagacgga actactggtc
     5701 cttgaaacaa ggaagttctt cagcaaaggg gttttcatgg actgggggac tggctcaggg
     5761 tgtcttgcct cggcggtgct ttccttctgc ccggaaagca ggtgcgtcgc ggttgaaagg
     5821 gaagggttgg cactgatgtg ggcctggaag aacctgcaca aactgggttt gctcgatcgt
     5881 tgccggttgt ggcattccaa agatccgtgg gaaatacctg tcccttcttc aagcctggag
     5941 atgatcattt ccaacccccc ctacatagct tcggaggaga ttgacagcct tatgccggaa
     6001 gtgtccgcgt acgaacctcg tccggctctc gatggggggg tggacgggtt ggatccttac
     6061 cgtatcctga ttccctactc tctggaaaaa ctccggcccg gaggggtgct tgcccttgag
     6121 accggggatg acaggggagc ccgggaaatt gagattatgg caaaaggtct tatgacgttg
     6181 gagtcgagag tcgaagacat tcacggtatc gcaagggttc ttgttttcag gaagccctgt
     6241 tctgctacaa tgtaggagcc tttcattcaa agaaaacatt caaggtcggg gagggaatcg
     6301 tttaaatgga aaaaagagaa ctggtgataa tcggggctgg ccctgcgggg cttacatccg
     6361 ctgtctatgg caagaggtcc ggtctggacg tgcttgttct ggagaagggc gttcctggcg
     6421 gacagataaa cgcaactgac gagatagaga actggacggg tgtcattcac gcgagcggcg
     6481 cggaactggc cgattctttc aggaaacacg ctgaacactt caaggccgag ttcagggatg
     6541 ccgaggtcac agggatagaa ctctgcgacg gatgcaagtt agtcaagacc ggtgatggtg
     6601 acatagaggc agaggcaata atcatcgcta ccggtgccag tttcaggaaa ctgggggtcc
     6661 cgggagaggc agagttcact ggcaggggtg tcagttactg cgcggtgtgc gacggagcct
     6721 tcttcgagga gcaggagata gctgtcatcg gtggtggaaa caccgccgta gaggaggcgg
     6781 tataccttac gcaatttgcc gataaagtaa cgataattca caggagggac aatttccgag
     6841 ccgatcgcgt cgccatcgag agagctatga ccaacccgaa gatagaggtc gtatggaatt
     6901 ctgtcgtgga ggagatagca ggcgaagaca tggtcgagaa ggtcgttctc aagaacgtca
     6961 ggaccggtgt ggtttccgac ctgccggtgt cgggtgtttt tgtctttacc ggaatggacc
     7021 ccaatgcttc tttcgtgaag ggtgttgtgg agtctgccgg caatggctgg atcgtcacca
     7081 acgagcggat ggaaaccagc gttgaaggga tcttcgcggc cggtgacgta agggagaaat
     7141 tcctgaggca ggttgtaact gcagcaggtg acggagccac ggcagctatg gccgcttcct
     7201 cctatgtctc ggagcagctt catatccaga gccttctcct ggagccggaa catgttaccg
     7261 catacatatt ttcaagtata gatgagggtc aggccagact tgcagccgac atcgaaaaaa
     7321 tctcttcagc ctcgaaggtt gttcagatcg acggatataa aaacaaaagg atggttgaaa
     7381 aattagggat cgctagactt ccctcacttg tggagttcaa agggggaaag ataacaaggt
     7441 cagagtttgc ccaggacgca gaaagcgtga aaaagtttct ttcctgaaaa attagcgcaa
     7501 gataaaccgg tctcttccat aaagattgta tcgcccgcgg ggaggtgcgt tttttgatcg
     7561 taacggttac gcttaatcct gctgtagacg aggagtttat cgttcctgag tttcgtcccg
     7621 ggaagtggtt caggaccagc aacgtgaaca ggtcgcctgg cggcaagggg atcaatgtgt
     7681 cctatatgct ttcccaggcc ggttaccagt cggttgcgat gggctttctc gcgggtttca
     7741 acggagaata tgtcagggat gctctcagga gaatgagaat aactaccaac tttgttcatg
     7801 tccaggggga cacccgggcc aacgtctata ttgtggatga ggttggacat gttgagacgg
     7861 ggatcgccga aggcggccca tatgtcccgg atgaggcgct ggcaaggttc ctggccaact
     7921 acgaaaggat gcttgggaga gcgcgaattg ttgtcctggg tggttctctt ccccctgggg
     7981 ttccacagga tatttacagg gatctcgtgg acagggccaa atccaggggt attcctacac
     8041 ttcttgatgc cgccggacct cccctgatga cagccataga gagtggcccg accttcgcca
     8101 ggatagacca caggttcatg gccagggtgg ccggggtctc tctgacatcg ctggataacc
     8161 tgatcgaagt ggtttccagg gtccatgatt acggggtcga atgggcagtc gcttcctaca
     8221 gatcctacgg cgccgtgttt tttgccccgg agggtatttt ccttgccgag tacgagagga
     8281 gaggactggt ttccctcttt ggtgccgacg atgctcttgt cgcgggcttt ataatggcaa
     8341 gggaagaaaa gatggacacc gaagggacca taagaatggc aacggcctgt gcctgggaga
     8401 actcgcttca tgtcgacaaa ggcctgagga gccgatcgga gatagaacaa ctcatccccc
     8461 aggtaaaggt tgttaaactg gattgaaggg cgacgtgggc gataacagga tcatcatgtt
     8521 cgtctgttcg ggcaatacat gccggagccc catggcggag ggcttcatga accgctgtct
     8581 tgaggatatt tccggagacc ttcccttcac ggcctgttcg tcgggaacga tggcatctcc
     8641 gggacttccc gcaaacccaa tggccttgaa ggctatgcag gaacatggca tagacatatc
     8701 gaggcacaga tcctccccca caggtatctg gatacctccg gaggagacgc tcttctttgg
     8761 tatgacccgc gtgcacagaa gggaactgca aagtatattt cctggacgct cttccagaat
     8821 atttctgttg ggtgaagctg ctggtttagg tctggaacag gaggaagttc ctgacccttt
     8881 cggtgccagc ctcgaacact accgggagat agccctcata ctgcgaaaaa tggttttggg
     8941 cctcctggat tctattaata gcgacccccg tttttctgcc tgaaaactcg caaccgggca
     9001 aacatgtccc ctctttctgt atgggcagct ttcccctaga atagttccat gaccctgtat
     9061 tatcaaaaga ggtgcagtag caaacctcaa aagggagaca tcatgagaga aaaaatccgc
     9121 aaaattgccc gggctctatt ggcagcgatc attctcctgt tgtctgttcc gtttttgtcc
     9181 gcagcatctg ccgacgatat cgaaaaagag ataaagctgg gcgaaaaagt ttcgaaacag
     9241 atagaagagc attgggagcg tgttgtcgac cccgtcaggg tcgccaggct ttccatgatc
     9301 cttgggaggc agctgccctt caccgaaaga gacttgccct ttgaggtgag gctcatcgag
     9361 gaaaagagac ccaacgcctt tgcgcttccg ggtggaataa tctatttcac gactggcatg
     9421 cttgatttct gcaggagcga cgatgagatc gccgcggtaa tgggtcatga attgacccat
     9481 gccgacaaga gacacgtaat gatccaggta gccaggaacc agaggctttc cataggtgcc
     9541 cttgctgtcc ttgtggctac acggggccag ggtgttgccc cgatccttgc caaccttgcc
     9601 caggtagcga taatgaactc ctacagcagg gatctggaga gagaagccga ccaggggggg
     9661 ctggagttcc ttaatagatc cggctatgag ccttcggctg ccgtaacggt catggaaagg
     9721 ctgcttgaag aggagatcaa gcgtccctat gtcgacccag ggatctacgc tgggcatccc
     9781 cggacgcgtg aaagaatatc ggaactggtc aaaatgatga agaccatggg tttgcctgtc
     9841 cggagaaaga ggtctcttca cctcctaagg acggagataa cagagtccga tgaaagaatg
     9901 gtccttgcga tcgacggaac gcctgtatgg tggggtgtgt cttcggaagg gacagaagaa
     9961 ttgctaaaaa aggccaatga ggcgatattg gtccatctgc agatggagac atcggccttt
    10021 gatgtcatga tcgctggtgt cgcccctgaa agaattctga aaattggtct cggaactgtt
    10081 gcaagggaga gtgagatcca ggaagatatg ccggctattg aagatttcag ggaaaacatc
    10141 ctggactccc tccaaaaggc ccgagccatt catcctgtgg ctgactataa ccggtaaatt
    10201 tccttttcgc gggaggcgat caaatgcacg taacccttta caggaggtac aggccgggtt
    10261 gtttttccga agtggtttcc caggattcgg ccgtcgatct tctcaggggg gagatatcca
    10321 ggggccgcca cgggcatgcc tatcttttct cgggtcccag ggggtgtgga aaaacatctc
    10381 ttgccagaat agtagccagc gcgctcaact gtacccgaag gagagatgac ggagagcctt
    10441 gccgcgaatg cgaaagctgt gtctccatat catcagggaa cagcctggat gtcgtcgaga
    10501 tagacggagc ctcaaacagg ggaatagacg agatccggga actgaaggaa cacatttcac
    10561 tttcaccctt ctcgggaaaa tacaaggtct acataataga cgaggttcac atgctcacgg
    10621 aacaggcctt caacgctctc ctcaagacgc tggaggagcc tcctgaaacg gtcgtatttg
    10681 ttcttgcgac cacggagcct cacaaggtcc cctcgaccat acggtcaaga tgtcagcata
    10741 taccgttcca caggatacca gcgggaagca tagttgtgag cctcgaaagg gtctcgatgg
    10801 aggagggtgt gaagctcgaa ggcgacgcct tgtgggagat cgccagggag tctgacggat
    10861 ctctcaggga tgctctatcc ctgctcgaac aggccattgg tactggtctg actgaaatat
    10921 ccctgaagga catccgcggg atacttgggg gcggaggaag aagcgaactt gaaaaactcg
    10981 ccgtacaact gagaaaaggc actgccgagg ctctgcttct cctggaggaa atgctggaga
    11041 ggggacttaa cgccgaaaga tttcttgagg gaatctttat ccttttccgg gatctcctgg
    11101 tagcatcaag gtggaaagat gagggtattt ctgcccttcc cctgagcgag ggtgaaaagg
    11161 cctttctcag agaggagtct cctttctgga agcaggaaga cctctggaag atactggaat
    11221 tctgcaccag aaatcttcca cgggcacgct ttggactaaa gacagagatc gccttcggca
    11281 tgttcctggg cctttttccg gaaggggaca aaaccgggaa aaaaccggga aaaaatgcaa
    11341 atataccctt gcaggatact tccccgtcgt ttcagataaa agatgaaaca gatgttatgt
    11401 cgactcagtc cttgatcgct tctggaaccg gaagagcaga agccgatgtc actccgaaag
    11461 gatctcccga acaatgggat gaactcctcg aatgttttcg agaggagaac attttgcttt
    11521 actctgcctg tgtggtggcc acgggcatca gtaaggaaga ggtttattcc ataatctttc
    11581 ccgaagaaaa cagatttgcc ttcgagatgg cctcttcgcc cagatgtctt gcatgcctcg
    11641 aatctatacg gataaacaaa ttcccccatt taaaaatgga tctgctgtgg ggtgaaaaga
    11701 aagtggaagt cgaaactgtc cctgatgaaa aagttgatga aaaacatgat gcaaaggccg
    11761 ttaatgaaga ggcccttccc cgaaagagaa aagccagggg ccatggcgag accagttcag
    11821 agcctcagac agagacggtc tcccagaatg gcagtaacgg gttgggagat attcagagct
    11881 ttttcaacgc tgaactcctg attctcagag aagaaaacca ggatctgccc gaagcgcagg
    11941 aaggagaaac agaagaacag tgaacaaccc tttcgttcat ctccatgtcc acacagaata
    12001 cagccttctt gatggagcca tcaggtgcga cgaccttgcc cgaagagcct cggagtggga
    12061 atgtccggct gtggctataa cggaccacgg ggcactttac ggggcggttg aattctacgt
    12121 ggcatgccgt aatgcgggaa ttaatcctgt tatcggatgc gaattatacg tggatccgag
    12181 agggcacacc cttaaggacc ggctggggcg gaactaccat ctggttgtct gggcagaaaa
    12241 catggaggga taccagaacc tggtcagact tgtttcgatc gccaacacag atggtttcta
    12301 ttacaagccg agggtggacc acgatcttct tgcaaggcac tcgaaaggtc tcatcgcatc
    12361 ttccgcctgc cttgccgggg agatacctgc ccttgtcctt gaaggcgacg aaaagggagc
    12421 cactgagagg gccgtcttct acagggacct tttcggggaa ggtaattttt tcctggaact
    12481 gatgcacaac tcgcttcggg agcaggctgt cgccaacaag gcgcttatca ggatctcaca
    12541 aaaaaccgga atacccctta ttgccaccaa tgatgcccac tatccggagg ccgaagacgc
    12601 cgggtggcat gacgtcctcc tgtgtgtcca gaccaactcg actgtcaata ctcccaaccg
    12661 ctaccgtttc ggggccgacg atttctacct caggtcaccc gaggagatgt ggaagttgtt
    12721 cggagccgat gtccccgatg ctcttacccg gactctggag attgccgaaa ggtgcaatgt
    12781 ggaattttct tttggatcct acaggctgcc agtgatagag gtaaaaaagg gttcaaccct
    12841 ggaagaggag ctggccataa agtcaaggga gggcctacag gatcgcttca gaggaagaga
    12901 tataccgccg gaatattccg aaagactcga atacgaactc ggggtcatca accagatggg
    12961 gttcgccggg tatttcctca tagtttccga tatcatacag gccgccaagg atgagggtat
    13021 ccctgtcgga cccggaagag gttcggctgc gggatctgtt gtggcgtggt ccctgaaaat
    13081 cacagagata aaccccctcc agtacgggtt gctgtttgaa cgtttcctga atcccgaaag
    13141 gataagcatg cccgatatcg atactgacat ttcagacagg ggcagggaca gggttcttga
    13201 gataatagtg gaaaaatatg gaaggaccca tgtatcccag ataattacct ttgaccgtat
    13261 gaaaagcagg gcggccatcc gtgatgtcgg gagagcgctg gatatgaaat atcccgatgt
    13321 ggaccgggta gccaagctgg ttccggccgg agcgacatcg ataggagatg ctctggagca
    13381 gtctgctgac ctgaaacagt cgaagcagga tgacccggcg gtctccaggc ttctcgaata
    13441 tgcctcgagg atggaaggta ttgccaggca ctgttcacag catgcggcag gggtcgtcat
    13501 aactcccgag ccgataaccg agatagtccc ggtcaggaag ataggcgaag accagatagt
    13561 gacccagtat gccatggaac ccatagagag gctgggtctt gtaaagatgg atttcctagg
    13621 gctccggaca ttgtccatga tagaggaggc ccttgagaac attgccggca gtggcaaaga
    13681 ggtacccgat atgtactcac ttccgatgaa agatcccggg accttcgata tgctctgtcg
    13741 cgccgaaact cttggcgttt ttcagctgga atcttcgggg atgagacaac tgctcagaca
    13801 gctcaagcct gactgcttcg aagacctgat agcggtcctg gccctttaca ggccgggtcc
    13861 actgggaagc ggcatggtcg agcagtacat agactgcaag catgggaaaa gggatatatc
    13921 ctggcttcat gaagaactga aggacattct tggggagact tacgggatga tcctttatca
    13981 ggagcaggta atgcagtgtg ctgcccgact ggcaggctat accctggggc aggcagacct
    14041 gttgcgacgt gccatgggaa agaaaaaggc cgatgtcatg gaaacccaga gaaaacagtt
    14101 cgtggcaggc tgtttggagc ggggagtaaa acccgcccag gccgagcata tcttcgatat
    14161 tattcaggag ttcgcgggtt acggtttcaa caagtctcac agtgccgcgt acgctatgat
    14221 aacctaccag acagcgtatc tgaaagccca tttccccagc gagtttttcg cttctttcct
    14281 gtcaagccac atccattcaa agctcgatgt gctcgccggg catgtaaggg ccgtgaggga
    14341 ttccgggatc aaagtggtac caccggacgt caacaggtct gtatctggtt tcagcgttga
    14401 cggagaacag ataatattcg ggctggacgc ggtatccaag gttggtcatc cttcggttga
    14461 acaaatagta accgtaaggc gtgacggcga ttttatatcc ctgtgggatt tcgttaacag
    14521 ggttgatacc aggatcgtgg gcagggcggt cattgagaat cttgtgaggg ccggggcgtt
    14581 cgattctata tgtacaaacc gcagacaatt gctggaatcc attccggtga tgcttgatat
    14641 tgtccagagg agagcatcgg agaacagcat gcaggtcctt cttgaccttg gtgaggagaa
    14701 agagtctgtg gagggcccgg ccatgccgga gatcgacgat tttgacccac acgagagaat
    14761 ggatatggag aaggaggcca cggggcttta tatttccggc caccccttcg atcactatga
    14821 gagggaagta aaccagtttg tcacctgttc gatccaggag cttgatttct ggaaaggagg
    14881 cgaatgcccc gtttccgtgg ccgggatagt agtgggagtc caggagaggt acacaaaaaa
    14941 gggagaccgt atggggatac tggagataga ggattcctgc gggaagatcg aagtaatctg
    15001 ttttcccagg atgtggactt ctttggcctg tccccctgaa aaaggttgcc tggtgatagt
    15061 aaatggaact ccccggaaca gggaaggtct ttccattatt gccgaaagta tcataccgag
    15121 ggagtcgata caggatcagg ccagatcatg gatcagactt aggatagaca tgaacggaac
    15181 cggaaccggg gtcaacatga gggaagtcct gagagaactc aagggttgcc ccggagacgc
    15241 aggtgttatc cttgatgttg cccttgatgg caaaagagcg atcctgaggt cccgggacct
    15301 taaggtcagg cccgatgatg gacttgcccg aaagattttc gagatatccg gcggaggggt
    15361 tgaagtggta taaatgaagt aggttttttc atcagatgct gtaccgggag ggttgaacat
    15421 gaacggggat gaatgctccg accgctcagt gctttcggat ttcatcgtag tcaaggctca
    15481 cgagaacgga gtttcggtga tcggactgac cagggggcac gataccaaat tcgctcattc
    15541 ggagaaactt gatgctggcg aggtgtggat agcccagttc acagagttta cgtcagccat
    15601 gaagataagg ggaaaggcta ccatcctgac tgctcacggg gccgtagaga gcggaaggcc
    15661 ggataatgaa acgggctaag atcgtctgta cccttggacc tgcctgcctt gaatacggtg
    15721 ttctcaagaa aatgattcag gcgggcatga atgttgcccg cctgaatttt agtcacggtg
    15781 atcacgaatc acacggcgct caccttgata atgtccgttt ggccgaaaag gaacttcgtg
    15841 tctccgtggg gacaatgatt gacaccaggg gtcccgaaat aaggacggga agacttgaag
    15901 ggcacgtccc ggtaacactc gtaccgggag gagtactgat actttcggcg ggatgggaag
    15961 gagacggcaa cacggaaaag gtcgggatca attacccaga ccttgcgcgg gagatatctc
    16021 cgggtgactc tctttttgtt gatgacggca cgctccacct tgtggccgag agtgtttcgg
    16081 gcactaacgt cgtttgccgg gtggcagtgg gaggagaact gggagagaac aaaggggttt
    16141 cagtaccggg cgtcagaacg tcccttcctg tgctttccag gagggacata gaggatgtcc
    16201 gttgggctct tgaacggaaa atggatttca tcgctctttc cttcgtcagg gacagggccg
    16261 atatcatgga agccaggaaa gtgatagaag aatcgggcgg ctccatgaga attattgcca
    16321 aaatcgagac cagggaggct gtggacaacc ttgaagatgt catcgaggtg gctgacggga
    16381 tgatggttgc aaggggagac cttggggtcg aaatgcccct cgaggatgtt cctcttgttc
    16441 agaaaaggat aattgacctg tgcaggtatc aaggaaaacc cgtaatagtc gctacccaga
    16501 tgcttgattc catgatacgc aatgtcaggg cgacaagggc cgaagcgagc gatgttgcca
    16561 acgccgttct ggatggggct gatgccctca tgctttccgg ggaaacagcg aatggagctc
    16621 acccggtcct gaccgtagag accatggcaa ggataatagc aaggaccgaa cgtgaggaat
    16681 ccccctggtc caggagactt ccggaaggca aggtcgaggg tattccggat gccgtcagca
    16741 aggccgcggc tatgatatcc gaccgtatgc aggcccgggc ggtgatatcc ctgaccagga
    16801 gcggaggaac ggctcagatg gtcagcaagt acagaccagg ggctccaatt atcggggcta
    16861 cgccggaaga aagcacaatg agatcgctat cgatggtatg gggggttacg ccactgcttg
    16921 tcccaatggg aaaagatctc gaaacagcac ttgattcggc tacatcgata gcacttaagg
    16981 agtcccttct ggatgaagga gatctggttg tcgcgacagc gggtttcccg ataggaagcc
    17041 ctggtacgac caactccatt caggttatgg ccgtggcaaa gacacttctc aggggactct
    17101 cgctccagaa gagagatgcc tggggcgtgg ttgtaaaggc tttttcttcc tccgaagccg
    17161 gagaaaaaat gtccgatgga gctatcctgg tagtccgaca gaccgacagg gattatgtcc
    17221 ctgccatgaa aaaggccgct gctgtaattt gcgaggaggg ggggctgacc agtcacgcgg
    17281 ccattgtatc cctcgaactg cgcataccct gccttgtcag tgccaaaggt gccctttctg
    17341 cccttgatga tggaatgacc gtgactgtgg acgggaggcg gggggttgta tacaagggcg
    17401 ttgtcaagct tcaccccgac gagaatgacc agtaacggtc tgactgatgt cgaaaggctc
    17461 tttgtttcca tccccttcga gggtcatgca aggagttgga aagaaatacc cggtaatccg
    17521 gaaaaacaga gcgcggtcct ggttcccgtt cttgccgaag gagaatgttt ttccctgatg
    17581 ctggtcgaac gatcaaagct tctccggagg catcccggtc agatctcttt cccggggggc
    17641 gcaagggatg aagatgatca tggtcctgtg gatacggctt tgagggagtt tgaagaggag
    17701 acgggaatat ctggcagcaa ggttgaggtt atcggtaacc tgactgaaga gtatgcctat
    17761 tccagtgatt ttatccttta ccccgtcgtg gggtttctgg gtcggagttt ttcggcggaa
    17821 gatatcaggc cggatccggt ggaggttgag agaatcttca cggtgccatt ggcctttttt
    17881 ttcggggtcc ccaaaatgga ggagttcttc cgtaatggaa tgactttcgg ttatcctgtt
    17941 tataccttca agggcctgcg tatctggggg gcgacggcct ggataataag gcgactggtt
    18001 attgccatgg gaaaagaccc tggcccgggt tgtctctgat gtctcttgtc ggaacccatg
    18061 tttctgcggc cggtggagtc tggcaggcca tagaaagagg tgaggccctg ggttgcgagt
    18121 ccatccagat cttcacgagg aatcagcttc aatggagagc ccggcccctt tccgagtctg
    18181 cctgcgacag gtttatgagg gcctggcgct caagcaggat aaagaaggtc gttgcccacg
    18241 cgccctatct tttcaatctt gctgcggaag gagacacaag gaaaagaagc atcgaatccc
    18301 ttgccgggga gatacagagg tgcgaccagc ttggaataga cgatcttgtg cttcatccgg
    18361 gatcccacct tggccttggg gaggaagcgg gaagaaaact tgtttccgat ggactgaagc
    18421 aggtgatagc cctgacggat tcatcgagaa ccagggttct ccttgagacc atgtccggtc
    18481 agggaaacac cctcggctcc acccttgatg aactggcctg gatctttgat gaggttgact
    18541 gctttccccg tctcgggata tgtcttgaca cgtgccatct ctttgccggg ggttacgaac
    18601 ttaggggcag ggtttcctac gaacgcctct gttcatcggt ggaaaggctt tttggtttag
    18661 ataacatcag gtgctggcac ctgaacgatt cgaagaaggg actcggaagc aggcttgaca
    18721 gacatcagca tatcggtgag gggatgatag ggagcgaact tttctcctgc atcgtctccg
    18781 accctagatg gaataacacg ccatgtcttc tggaaacacc caaggacggc cctggtgacg
    18841 agggcaatct tgcccttctg agaaaaatga gaggttttta aactgggtat atcccttcag
    18901 gaaagcgagg cgtacctatc gtgcaatatc ctgaaatttc cgttcgaacg aatttaatag
    18961 ccgaaaacgc gaaaaaaatc gcggaacttt gtgccccgtc ggggataaag gtatgggggg
    19021 tcacgaaggg agtaagtgcc caaccctccg tagcggcggc aatgatcgac gggggctgct
    19081 gtgggctggc tgacagcagg atcgaaaaca taaggaagct gaggctcttc ggaatcaggg
    19141 aacccatgat gctactgagg atccccatgc ctggagagat ggatcatata gcggagtttg
    19201 ccgatatctg cctggtctcc atgcccgaat cggtccttat gcttgaagag gcatgtcaaa
    19261 gggcaggcag ggatatggga gttatagtaa tggtcgacct gggtgatctt agagagggaa
    19321 tatggcccga cgagaccgca ccgatggctg aagcccttcg ccggtgccgg aaggtaagat
    19381 gcaaaggcgc gggaagcaac ctgggatgcc tctcgggagt ccttcctacc cccgtgaatc
    19441 tgtccctgct cgtagatgtg accagggaac tgggatcttt cctgggatat cccatggaat
    19501 acgtgtcggg cggtgcgaca tcgtctcttt cgctggtgga gaacggaacg atgccttcag
    19561 gggttaatca gctgagagtc ggagaagcca tattgcttgg caccgacgtg accagggaca
    19621 gggttattcc atggcttcac agggatgcga tcacattctc ggcggaggtt gtggaggtaa
    19681 gaagcaaacc atcggtccct atcggtgaaa cgggatccga tgcctttggc aacaagcctg
    19741 tcttcgagga cagggggacc aggagacggg tcattgtagc tgccggaagg caggacgtga
    19801 gacccgaggg acttgtgccg atctctccag gaattaccgt gatcggcgct tccagtgatc
    19861 acctgaccct tgatgccggt gatctcgatg gcgaggtatc tccgggtgat gtcattaact
    19921 tccgcccgga ttacggtgcc gttctcgcgg cgaccacatc tccttatgta agggttaaaa
    19981 tatctctatc gtgaaatagc gggaggttca attgacagga atgatcggac ccatccgttg
    20041 gcaggacttc attgacatcg ccgtaatagc ctttattgtc tacaggctgc ttctgttgct
    20101 tgtcgataca agggcaatgc agctggtgaa gggccttctg gtgataggtc ttctcttcgc
    20161 gctggcgaga tgggttcacc tggctgccct gtcgtggata cttggaagat tgctaggggt
    20221 cttcattatc gcgatcccca tagttttcca gcccgagata agaagaatgc tcgaggaact
    20281 tggccgcggg aatatatggc gtcgccggca ggccgaggaa aaggcggctc agcttacaga
    20341 cgagatcacc cgcgcgctgc tcttcctcca gaataaaaac ataggggcga taatcgtatt
    20401 tcagaggagc acggggctca gggatatctg gagatctgca gttcagatag gtggagatat
    20461 ttcccaggac cttattctct cgattttctg gccgggaaac cccctgcacg acggagctct
    20521 tatacttgac agggagaagg ttattgcagc ggcctgctat cttcctctga cggagaacag
    20581 cagcctctcg agatggatgg ggaccagaca ccgggcagcc ctgggcgtta ccgaggtctc
    20641 cgacgcgatg tccctgatag tttcagagga gaggggagag atcgctctgg caattaacgg
    20701 ccacctttcg agagaactga aagaggctca gatcaggagg cttctcgcgc actatttttc
    20761 agaggcgccc gattcgggtt cggcaataga gggagtaaag ggcagattcg ctgccctttt
    20821 ttcgggaggt accgggaaaa atgatcaagt ggattgacag gctgctttct tccaggcttt
    20881 ttctaaaggg gatatccctt cttgttgcca tatttgtctg gtactatatc acggccgaca
    20941 ggggtacaga gatagtcagg acggtaacgg tcccccttga atatctcaac gtcccttcgg
    21001 atatgtcggt ctcaagcagc gtaagagatg ttgatgtcca gatctcgggt gcccgtgaat
    21061 cggtcttctc ccttggcgcg tcagtcgctt cccaggttga ccttctgggt ctggaaccgg
    21121 gtcttcacag gagaccggtc cagacattgc tcccttcagg ggcccggttg gtggaagtgt
    21181 cacctcaaaa cgtcgagata gaactcacaa ggatggggtc tcggctgatg cccgtcatgt
    21241 ttgaaccgcc tccggacctt cccccgggat acagggtaga agatgtgtct gttatgcctg
    21301 gggaggtttt ggtaaaaggt ccggaagaat ccatttctct tcttgaaaat gtcgtggtcc
    21361 ttccgactgc cgggcagatc cttgaggaga agaacctgac tctgccaata tccctttccc
    21421 ccggccagga gatggacagg gaccagttca gggttgaccc gtcgcaggct ttgctttcgt
    21481 tccggctggt gaagggtttc cccaggaaga tcgttccagt caggcttgag atgaccgggg
    21541 ttccatcaag ggattaccag atagaggcgg tggccgtaga ccccccagag gtaaccattc
    21601 agggtccgct tgagtcagtg gaaaggatcg acgagttgtt tcttccgcct atagatgtaa
    21661 caggcatttc tcgtgatttt tcggcggtga tacgcctggg cgatccttcc gatgatgtcg
    21721 agatcgtcgg tgaaaaaaac gtcaacataa gaataatgct ctcaggcaag gtggagaatc
    21781 gcctttacgc caggatcccg gtgcgggtat ctggcaagag catctatccc gggtggagag
    21841 tggaaccttc cgaagtgagc gtctacctcg aaggatctcc ctcagatctt gacgcagccg
    21901 agaccagggg tgtccccgtc gaggcattcg tggaggttac caacctggtg tcgacaagga
    21961 taagggtccc cgtccagtac gaggtcaggg gcaagggact gaaactgtcc cgggtagaac
    22021 cttccaccgt gatggtatat gcccttgaag aatgaccccg gccccgggag aatggagtgg
    22081 tccaatgcca gagagcggag aaaaaagccg ttgccttttc ggaaccgacg gagtaagaga
    22141 tgttgccaac aggggtatta tgaccccgga gatggccctc aggcttggca gatcctatgt
    22201 tctttttctt gttgaaagag gaatttccag accttccata gctgtcggac gggacacccg
    22261 gagatcagga ccgatgatcg agtgcgccct tttgtcgggt cttgcgtcgg ccggagcaga
    22321 tgtaacatct ctggaagtga tgccgactcc gggtgtcagt ttcgccgtga ccaggctctc
    22381 ctgcgatggc ggcgcggtgg taagcgcttc tcacaacccg tcggaatata acgggatcaa
    22441 gttcctgggg cccgatggtt gcaagctgaa tgatagtgaa gaggccgcaa tagaagacta
    22501 ccttggcgac atcctttttg acgaatggag accgacgggt ccctcggtgg ggactgtctc
    22561 gtacggtaga gagctctcct cggattattc caggtgggtt ctggatacct gctgtattga
    22621 tgtggatccg gacctggata ttgttgtgga ttgctcgaac ggcgcggcgt catatatagc
    22681 aggggaactt ttcgggaaat gtttccgtag ggccagggtc atagaaaata tccctgatgg
    22741 atcaaacatt aacgaaaact gcggggtcat gcacatgaac agtctcgctt caattgtgac
    22801 ttccggggga aacagtcttg gcatggctct ggatggtgac gctgacaggg tgctttttgt
    22861 tgatgccaga gggcgcctga tcgacggtga tatcatgctc tgggtgatcg gaagatggct
    22921 atcaaaagaa aaaaagatcg gaaaaggggt agttgcgacg gtgatgagca acaaggccct
    22981 tgaggagagg ctcgggtctg aagggatacc cgtgacaagg tgccccgttg gcgaccgtta
    23041 cgttctcgat gcaatgagaa ggtcggggtc acttctcggt ggagaacagt ctggacatat
    23101 gatagccggg gatttcgttg ctaccggtga cgggttatgc tcggggctgc ttttcctgac
    23161 ggcatgtcgt gttctgggag aggacacatc taccctggtg gatcgttttg gaaggtaccc
    23221 acagatactc aggaattttc attctgccga caaagatgag ttcatgcgcc gccctccgat
    23281 cgaagaggcc gtggaaaggg ctgtcaggga ccttggagat atgggccggg tcctcgtgcg
    23341 cccctccggt acagaaccgc tggtaagggt tctcgtggag gcaagggatg aggcgactct
    23401 cagatctgtt tccggccccc tttttgatac catcgccgga gaggaaaatt tggatagaca
    23461 ggatggatct ctggaggtga ctccccgatg aaggggaata tcaggaaatg cctctttcct
    23521 gtagcaggta tggggaccag gttcctcccg gcgaccaaag agatacccaa ggagatgcta
    23581 cctcttatcg accggcccct aatccactac ggagtagagg aggcggtctc ttctggctgc
    23641 agtgacgttg ttttcgtcac cggtagggga aagagggcca tagaggacta tttcgacagg
    23701 tcctgggaac tggagcacgc gcttgttgaa aaagggaaaa ccgaacaggc agccatgatc
    23761 aggatgatca gtgatatggc taacttttcc tatgttcggc agccggaacc gctggggctt
    23821 ggacatgcgg tgctgtgcgg tgagtccttc tgcgggaatg attattttgg ggtcattctg
    23881 cccgatgatg tgatggtatc cacgccggga gaagatcccg tccttgggca actgataaaa
    23941 atccacaacc ggtttggtgg ttctgtgata gcactggaag aggtcccctt cgaagaaacg
    24001 tatcgctacg gaataattga aggggaaaca gaggaagacc gtgtctaccg ggtgagagac
    24061 cttgtagaaa agcctgaaaa gggaaaggct cccggaaggc ttgcagttat ggggagatat
    24121 gttctttctc ccggtatttt tgatcatctc agatccgttc ccaggggtgc tggaggagag
    24181 atacagttga cggacgccat aagggccctt ctgaaagagg aaacagtcag gggagtcctg
    24241 tacaggggtc gaaggttcga ctgcggaaca atggaaagct ggctcagggc cacggtgtca
    24301 atgtccctgc agagcggtga tcttgccgag gttatcctta aggaggtgga gcagtttcga
    24361 ccttgtgaaa accgtttatc cggttctgaa agatactgag cacagaaaag gttagcgcct
    24421 gaactggcgt ttgccagttg acgaggcccg gggagtatcg atttttcggc ggatgccccg
    24481 gaggcaggaa cggcaaggcc atcaagccgc cccacaaaac cggggagtga tctccgggac
    24541 aaaaggacgg cacccgttcg tcattgaagg aaaggcggtg cccatgctgt catgtgtggg
    24601 atagttggtt atgtgggcaa aagaaacgct tcggatgtgc ttgttgacgg ccttaaaagg
    24661 ctcgaataca gagggtatga ctcggctggg ctggcgctcc ggacagagga agggattctt
    24721 attctaaagg aggtcggcaa agtcgccgac cttgaggcgc ttctgggaga aagctcacca
    24781 gtttcgaccc aggggatagg tcataccaga tgggccaccc acggaggggt tacccaggcg
    24841 aatgcccacc ctcacaggga tggcagcgga aagtttgcca tagtccataa cggtattatt
    24901 gaaaatttca tggaactcaa ggaagagctt gaaatggaag gggtcacgtt tctgtcagag
    24961 actgataccg aggcagtagt tcacatgatc gcccgtacat attccgataa aggagatatg
    25021 ctggcgacac tggtcgctct ccagaaaaaa ctccggggtt ccttcgcgct tgtggttata
    25081 cagcaggact ttcccgacag gatctactgc gtaaggaggg gttcgccact ggtggtaggc
    25141 tccggggagg gtgaatcctt ctgtgcttcc gatgtgccgg cattccttcc ctttacaaga
    25201 aaggtccagt atcttgagga gggtgacatt gccgagatat ctctcgacgg aataaagatc
    25261 tggaatgagg atggcgtact tcagaacaag cctttcctta ctgtggactg ggatgtctcc
    25321 atgaccgaaa aatcggggtt ctctcactat atgctcaagg aaatacatga gcagggtatg
    25381 gttctgcgtt caaccctcaa gggaaggatc caggaaggca ggacttccat tgaacatgaa
    25441 cttggctggg acccggaaat ggtcgcgagg tggaacagga tccatgtcat cgcatgcggc
    25501 acttctttct atgccgccca ggtagcggaa agagtgttcg agaagtggac agatttcgat
    25561 atcaaggtag atatagcgtc ggaatacagg tacaggcata tcaggtgtga ttcttcgaca
    25621 ctagccatat ttgtgtccca gtcaggtgag acggcggata cgatagctgc ccagagaaaa
    25681 gccagagctg agggggctca ctgtctggca ataaccaacg tcaggggctc gacccttgcc
    25741 agggaggttc acagcgtgct cctcctgaaa gctggtcccg aaataggtgt tgcggcaaca
    25801 aagacttttt ccggccagat ggctgccctt tttcttgtgg cgctgcaatt gggcagaatg
    25861 aggaactctc tctgcccgga agaggaggag cggcttctga acgagtttct ccagatacca
    25921 tacaaggtgg agatactcct ggaaagggaa caggagataa aggaactcgc ggagaaatat
    25981 gccgcctcgg agaactttct gttcattgga aggggaatat ccttccctgt ggcattggag
    26041 ggggcgttga aactcaagga gatttcctat gttcatgccg aggcctatgc cgccggagag
    26101 atgaagcatg gccccatagc gttgctcgaa cctgcggttc cggtgctgtc cgtcataccg
    26161 agagacggtc tttacgaaaa gaccctctcg aacattcagg aagccaaggc cagaagatcg
    26221 cctctgatcg cggtctgcac tgaaggcgat caacttatat caaaacatgt cgacgactgc
    26281 gtttacgttc cctggactga cgaagagttt tcgccattcc tcacggtagt gcccctgcag
    26341 tttttggcat ttcatatcgc ccttctcaag gggagggaga tcgatcagcc gaggaacctt
    26401 gctaaaagcg ttacagtcga gtaagagtaa tttttcgagg gcggggtttc ttttaatacc
    26461 ccgcctgcgg ttcttcggta aagggatagt aaaccctgag atgggtgatg tctctgaaag
    26521 cgtggtcttc tctcccggag acttttggca tctgccccca gttacccccg cggatcctca
    26581 ccagcaccag aggttttctc ccgtatgttg cccaaaggtc ccacagcttc tcgccggtag
    26641 ccgttccagg tacaagtccg cccgaaaaaa gtgggaccgt ccccttgagg gtctgggctc
    26701 ccgcgtcggc ggatgcgact atcatccact gggtcatttt ccacgctccc tgcatctcac
    26761 tggaagatgc gtgctcaagg ggcattatct ggaaaccccc gatcaaccct tcgggtgagg
    26821 tgcagatgtc tattactccg ggatgattag ctcttatcct tcccgctgac tggtagaggg
    26881 tgccgtcgaa cctgccggaa cctccgaggg gtcttattac cctggcttcc acctttggtc
    26941 cgctgtcata ccatgcagtc agcctccctc cgggtctgtt ctcgatctcc agatagtacg
    27001 gattgtccga agagggtacc cggatcagaa gtgtatcccc ctcaaggggc aggcgggcgg
    27061 tctcaggagt ggttttgccg gagggtatga tcagaaccgc gcttcccgaa gaaggggccc
    27121 aggcgccgaa tattgaccta cctgccctga tgtctgtcac tatggccgac ccgggaccgg
    27181 cggcgggggc gatcgtttcg gcgggcagta tgctcattgt ccttccgcgg cctttttcaa
    27241 cagaaagtaa aatgtgaagg gcgtttacgg cggtcgcggt aacagttccg ggatgtcccc
    27301 aggcggaagc ggtgaaactg ggccaacgcg acccatcggg cagggcgacg acccttcctg
    27361 ctgtctgagg gggttctccc ggtctgagga tcgttatgtc gccccctgga acgcatttca
    27421 gggctattgt gaaaggtccg tcaattgcag ggaaggcggg ggactgcaag agcagcacaa
    27481 aaagcacaag aaacagtgag aaacgtttca agagccattc ctccattttc cggcactgaa
    27541 aagaatcatg atgaatattg gagcgggaac cagtggggcc acggcgaccc ataacggggc
    27601 atcactcaga agcgacagaa aagatgcaag aatgaaaagg gcaaggaaaa gccagaaaag
    27661 taacacctgt ctcttttcac cgctccgggc tgccaaaact ccgaagatgg cactcatcac
    27721 gaaaaaactg atgacagcgg aaagagatat ccatctcatt gccagttttt gctattcccg
    27781 acaggttccc tgtctttttc tctctcccgt gaaatgcagg cggaacaggt tccgtagcag
    27841 tagacctgga tgctctcgac aatttcgccc gaggcctcgg tgatcttttt cagtgttccc
    27901 gaaggaaggg ggatatcgaa gacgtgcccg cactttctgc atcggtagtg agcgtgaggt
    27961 tcggggttgg ggtcaaagaa aaccctgtgc ccgtctattg taagtatctg caaaagtcca
    28021 gcgtcctcaa ggatctgtgc cgtgctgtat attgtcgcga atgatatggt cgggtgatct
    28081 tttttcatct ccctgaagac ctgctcggcg gaggggtggt ctttccttcc cttcaggacc
    28141 ttcagtattc ctactcgctg agaagtaatc ttgagacctt tgctcctgat cttttctgtt
    28201 ccttcttcaa ttgtccacat ggtgaaaggg acctccccgt tgcggattcg gaatgatgta
    28261 aatattctgt aattataata acaaacagac caggagaaaa gagaagggtt tttgtgtctg
    28321 atatttgcct tgacatgtct ccgcagtgtg aggatataat tgctgggcct gtgtaatggg
    28381 gtgtagccaa gctggcaagg cgacggactt tggatccgtg atcgctggtt cgaatccagc
    28441 caccccagcc agaaccaaaa aacaggggga agctatttag gcttcccctt tattcttgag
    28501 tgtctttggc ccgttgggag ccagagacag gggacaggtg gccgttgaat ggctccgtga
    28561 tagaatcatg atggatgatt agccttgggg gggattgtga tggccagatc aggaactggt
    28621 gttcttatac tcgcagcagg caaggggact cgcatgaaga gcgatacacc caaggtcctt
    28681 ttgccccttc ttgaggaacc ccttctgtat tacccgctgg tttcaacgga ttcgccggat
    28741 ttctcgggca ggggcgtagt ggttgggcac ggaagcgaca aggtgtcggc ctatcttgaa
    28801 aaatcgtggc ctggttcaga ggtaatagaa cagaaacaac agcttggtac cggtcatgcc
    28861 gtggctgctg ccaggaaatg gataagcagg ttcgatcatg tactgataat tccgggtgat
    28921 gtccctcttt taggcaggga aactctttcc atgcttcatt ccaggcatct cgaaggagat
    28981 gaggattgtt cctttcttgt cttcgaaccc gacgatccat ccgggtacgg acgagtaatc
    29041 cacacagaaa agggttttcg catagtagaa gaaaaggatg caatggagca tgaaaaaaag
    29101 tgtggacggg tcaacggggg gatctatgcg ttccggacca cggtccttct ggagcatatc
    29161 gattccctcg gcaaagacaa tgcccagggt gaatactatc tcaccgatgt tattcatgac
    29221 ctgtgccagg aagggaaaaa gatccgcatg ttgttatgcc ccgacccgga agagatcgaa
    29281 ggggtcaacg atccgcagca actggatgtc atgtccagaa ggcttcgcaa aaggatcctt
    29341 gcgaagcaca tgagttgcgg ggttaaatgc gtagactcgg agtcggtatg ggtaggtccc
    29401 ggggtaagac tgggggagga cgtgatgctg gatccctttg ttcagttgtg gggtgacacc
    29461 tctctgggga agggttcccg tatcggaagt ttctctattg tcaggaactc gattataggg
    29521 gaaaatgtcc atttgttggg tcacgttgtc ctgaatgaca gtgagataca gaatgacgcc
    29581 cgcattgggc ccttttcatt tattcgggag ggttcggtac tgcgggaggg tgcttacgtc
    29641 ggcaagttcg tcgaggtaaa gaaaagcgtc ataggaaaaa ggtccaaggt cccacacctt
    29701 tcctacgtag gggacgcgac cctcggtgag gatgttaaca taggggcagg gaccattacc
    29761 tgcaactatg atggcgtaaa gaaaaacccc tcagtcatag gtgatgggtg tttcgtaggg
    29821 agtgacacta tgctggttgc tccggtaacc cttggtgagg gggcctatac agcggctggt
    29881 tcagttataa ccagggatgt tccgcccgga agcctcgcga tcggcagggc gaggcaaaaa
    29941 aatattgaag gatggtccgc aagacgcaag ggacccaaag aggaaggaga atgaaagtgg
    30001 cctcaaagtt gcgggaactg aagatttttt cagggacggc ccacccccag ttcacgaggt
    30061 ctgtatgcga gaatctggga gtaggagttg ctacggccag tcttttccgt ttctccgatg
    30121 gggaaattgg cgtttcggtg gaggagagcg taaggggggc cgatgtatat gtcgttcagc
    30181 caacctgtga tcccgtcaac gaaaacctga tggagcttct ggtcatgatc gatgtgatga
    30241 ggagagcgtc cgcgtacagg gtaaacgttg taacgcccta tttcgggtat gcccgacagg
    30301 accgaaaaac caaggcgagg gaaccaataa ccgcgaaact tgttgctaac ctcatcgaaa
    30361 aggctggagc ggacagggta attgcggccg atctccacgc aggccagata cagggattct
    30421 ttgacatccc ggtggatcat cttacgggga tcccgttgct tgcggcctat ttcagggata
    30481 ttttcaagaa cgatcttgaa aaagacaaga tagtggtaat atcccctgat atcggtggag
    30541 ttgtgagggc aagaaggttt gcggttcacc tcaacgccga ccttgccatt gttgacaaga
    30601 gacgatccca cgaagtggca aatttcagcg aggtcatgga aattatcggt gatgttgatg
    30661 gcaagatcgc cattcttgtg gatgacatca tcgatacggc aggaacgatg gttcaggccg
    30721 ccgaagccct gatatcacgg ggagccagaa aagtctacgc ctgcgccact cacggcgttc
    30781 tttcggggcc ggctatagac cgtatcgcgg gatcaccgat cgatgaactt gtccttacca
    30841 acacggtacc ggttccagaa gagaagaaca tagacaagat aacaaccctt tcgatagcac
    30901 cacttttcgc ggaggccata agcaggattc attcggatca ttccgtcagc attcttttca
    30961 gatagaccca ggtcggttca tcgaaaaagc cgacaaaatc aaggagggag cttcatggat
    31021 acagcagaaa gaatcaaact ggaaaagaga gagacttcgg gcaaacaggc ctgcagaaaa
    31081 ataaggaacg aggggaagat cccgggggtg ctttacggtc cgggaatcga tggtgccgtt
    31141 cccttccagg tctcctccgc cagaatttca ccccttgcca actcgggacg ctgggagaca
    31201 gcccgtcttg aagtggatgt gccgggtggc aagaaggcct cctgtctcat gagagatctt
    31261 cagagagatc ccctgacggg aaagatcctt catgttgatc tcttccagct gaagaaggga
    31321 cacaagatca tggtcaacgt ccccgtcgag atagcgggaa gggagaaatg tatcggagtc
    31381 aaacagggtg gcgtgttcga gcagatgatc cacgagatcc agatggaagt tctccccaag
    31441 gagatccctg attcgatcgt tgtagacatt tcatcgctca aaattgacgg gtcagttcgt
    31501 atatctgatc tcgaaatgcc gggaagcgct gtgctcgatc tttctgaaga aacaattgtg
    31561 gtagctatag ggcatgcaag ggccgaggcc gagaagactg atgaggaaga ggaagaagaa
    31621 actgaggttc aggtagtatc gaagggcaag gccaaagaag aggaggcttg agccctattg
    31681 tgattctggt cgcaggtctg ggcaacccgg gttccaggta cgcctggaca cgacacaatg
    31741 cggggtggca tgtgatcgac cttctcaggg agaggttgtc cgctcccaga tggtcctcta
    31801 cccgcgagag tgaaatttgg ggtccatgta aggtcgatga ccacaaggtc tttttgctga
    31861 agccaatgac ctatatgaac cttagtggcg tagctgtgaa gagatctgtc cgtagcctgg
    31921 ggatcgatct ggaagatatc attgttgttt atgacgacat tgctcttcca tttggacgaa
    31981 ttcgcttcag gcctgggggt tcggccgggg gccaccgggg aatgatctcg gtaatagggc
    32041 atatggggtc tgccgagata gcgaggatac gcatcgggat cggaggcagt gatcctgacc
    32101 gcgaactgat agattatgtt actgacccct tcacccggga ggaactctct cttttgccgt
    32161 caatttgtga cagagcctcg gattcggttc ttttgtggat atcaagggga cccgaggaga
    32221 ccatgagagt gaccaatgcc cctcccgacc atccacgaga gaacagatcc ggtgaccgag
    32281 actgatatgg cggggggaac ctcgccattc tttttatgga gaaaacagta atgaacggaa
    32341 tcgacagaaa aggatcggca gattctctga gtagcatttc caatacagtt accaccggaa
    32401 cgaatacggt tcacatcccc gccggcaccg ctgcgtggtg ctgggctatg agggatctgg
    32461 aagggccggc cctggtaatt cttcccgaca ggcgggtttt ggctcaattc gtcgctgact
    32521 gggaacaact tgttccggaa aaagacatcc aggctctatc cgagataccc ctttccaaag
    32581 aactgataat taacgatgct ataagaatgg agcggggatg cagcacaatg aaatggctcg
    32641 cgtctggaga tctaatggca gccaccgctc ccgctgtaat ggcgccgctt ctgcttccgt
    32701 cggagaggat agagatagga gagggcatcg aggcgggcag ggatgtactg ttggaatggg
    32761 cccttgcctc cgggtacgaa cgatcggacc ttgtttggca ggagggccag ttcgtcagca
    32821 ggggaagtat tgttgatctt tttgacccct cctacaggta tcctctgagg atcgagtttt
    32881 tcgacgacag ggtggaaagt ataaggatct ttgatcctgc gacccagaga aacatatcgc
    32941 ctgtgggtgt cacgaaactt ctttcgctga agcggggaac ccgtaaaacc tttcaggcaa
    33001 tgataccgcc ttctgccaag attattttcg tggaaaggcg ggcgatatca cggtctgccg
    33061 aggcgtacaa atggctgtgg gatggcattg cgggcaatat cggtattgat tcccttgaag
    33121 aatgggaaaa ggtcgaatac ggtttcagga gcaacagatc ctttcatttg acaaaagcaa
    33181 ttgagggctc ttcttatcgg ctggatattt cggaaatacc ctcgttcagg gggaaacttg
    33241 atgcggccga gtcattcgta agaggttgga agaatgacgg tttccatgtc acggtgttat
    33301 catccagcga ccttatgctc gaatgggccg ggataaatgg tgttgtgggg aagagaggaa
    33361 acatttccgg cggtttctgc gaccggaccg aaaaaaaggt ctttttgtcc gactccgacc
    33421 tcacaggtat cgtccccgtc atttccaggg gaaaggttac ccggatccct cccagagagt
    33481 gggaggaaag gatcgaggat caggactacg ttgtccacga agattatggc atcgccagga
    33541 acctgggtat cgaaaggatc tcctcaggcg gcatggacag ggactgcctt gttctccagt
    33601 tcgccgaaaa ccggcggctt atgatgcctc tggtgcagtt gttcaagatc tcaccctatc
    33661 cgcttcctgt cggagaagat cgtgaactgg acagcctgcg agggaaagcc tggaaaaaga
    33721 atgcggcgaa ggccagggaa agggcaaagg aagccgcccg tcagattatt tccctatacg
    33781 cagcgaggga gtcggcatcc aagatacccc ttcctcccga cggagaaata atgcggaatt
    33841 ttgaaaaggg ttttcctttt atcgagaccg cagaccagat atcggccata aaagccgtca
    33901 aaaaggatat ggagaacagt tttcccatgg agagactact ggtcggggat gtcgggttcg
    33961 ggaaaacaga ggtggcgttc agggctgcaa ttaaagcggc cgagtcgggc aaacagaccc
    34021 ttttccttgt tcccacgaca ttactcgcat cacagcatta cgaatcattc atatccagga
    34081 tcaccggcct tcctgttcgg agtgaagtgc tttcaaggtt tgtgccaaag aagaggcagg
    34141 atgaggtcaa aagagatttg gaatcgggcc ttgttgatgt tgttttcgga acacacaaac
    34201 tgcttcaggg ggaccttaag gtaaaaaatc tcgggctgat aataattgac gaagagcaca
    34261 ggttcggggt gcttcacaag gaaaggctca agaagatcga cccctcagtg gatgttttga
    34321 gtatttctgc aacacccata cccaggacat tgcagatggc cctgggtgga ttaagaagca
    34381 tttcggtcat atccacgccc cctcacaacc ggatgcaggt aatgacctat gctggaccct
    34441 ggagggacga tctgttcagg gaatccgtga tcagggagat cgccagggga ggccaggtgt
    34501 tctatgttca taaccgcatc gagagcattg agaaagtggc ctccggtatc aggagacagt
    34561 tccctggtgt ggtggtggac attgctcatg gcaggatgga cgagagggca ctggaagagg
    34621 ctatgtccag atttgcttca agggaaacgg atcttctggt atgcacgacg attatagaaa
    34681 gcggtcttga tctgcccggt gccaacaccc ttgttgtaga caatgcccag gatctcggcc
    34741 tggcccagat gtaccagttg cgaggtagag taggacgccg ggaagcgcag gcttacgcgc
    34801 ttttctttta cccggccgac gtcagccttc cccgtgaggc catggagagg cttgaggcta
    34861 ttgctggtat ggacggagat gaaggtggtt ttgccctcgc caggagagat atggaaataa
    34921 ggggtggcgg acagatagcc gggacgaggc agcacggaca cgcggaaaag gttggttacc
    34981 actcctactt caagatgctt gaggaggaga tatctctggc atcagacaaa aaaacggttc
    35041 cggtggtcaa aatggatatt cgtttacccg tggttctccc aagcacttat gtaccccagt
    35101 caagtgtgag aattgttatt ttcaggaggt tgctcaaggt ttcggatatt aaagagacgg
    35161 aagaactgaa aaacgaactg cgcgaaaggt tcggcccctt gcctcaatca gtggagttta
    35221 tgctggcttc cgctgtgatc aggacactgg gggcatatca tgggttacaa tacgttcgtt
    35281 gcagtctcaa agaaacatgt gccgcagttg atgtggacca tccatcccgc ctgccgcggc
    35341 gcgcggggtg gatgcaaaag gcaaatggga ccattgtcgg ccccggcgga tacaggggaa
    35401 tgctggagct tgcgggtatg atagaagtat cggaagaagt taaaacggag gcgcggcaat
    35461 gaatgatcac gaaaaggtca gcttgaggat aaaagaactg cttgtgataa tggagaagct
    35521 cagaagcccc gggggttgtc cctgggacag ggagcagact atgcactctc tccgcccctg
    35581 tatactagaa gaagcttacg aacttgtgga ggcaattgaa agagacgatg atccttccgt
    35641 ttcagaggaa tgtggagatc ttcttctgca ggtagtgttt atctcctgcc ttgcggcaga
    35701 ggaaaaccgt tttggcctgg cggaagtaat tgaaggttta aacagcaaac tggtcagacg
    35761 tcatcctcat gttttcgggg aacagaagat tgataccagc gacgctgtcc tgagaaactg
    35821 ggagcggata aaacaaaaag agagaaaggc caggaataac gacacgtcgg tttttgctgg
    35881 tgtacccagg aacctgccgg ctctggcaag agctcgcagg atccaggagc gtgcggctaa
    35941 gaagggtttt gactggctgc cggaagagac cgaaccggtc atcagcaaga tccgggagga
    36001 aattgaagaa ttgtccgggg cagtggcatc tggcaacggt gatagcatag aggaggaaat
    36061 gggagatctt ctctttgcca tggtgaacct ttcgaggcgc cttggttatg aagcggaatt
    36121 cagtctgcag agagcaaaca ggaaatttat ggacaggttt cgttttatcg aagagagtgt
    36181 ggagtccagt gatcggaact ggtctgatta ttccctcgaa gaacttgagg atctttggca
    36241 gaaggcaaag aaaaacctga accttgatag ttctgttgac gctactcatt gccggaggtg
    36301 aagaaatgag tgagaaaaga gaaaagggta gtgctgcagc agcaaaagaa gtaaggccgg
    36361 aaatggtgga aaagaccaaa aacagccctg gccgaaagat cggcattacg gaaaccgcgt
    36421 tgagggatgc ccaccaatcg ataatggcca cccgtcttcg aactgaggac atgatacccg
    36481 ttgccgaaga aatggatgat gcggggtatc attccctgga agtctggggt ggtgccacct
    36541 ttgatacatg catgagattt ctgaatgaaa acccctggga aaggcttcgc actttaagga
    36601 agtgtttcaa aaagaccagg cttcagatgc ttcttcgtgg tcagaatgtt gtcggctaca
    36661 gacactatgc cgatgacgtg gtgcgggaat tcgtcaagag ggctgttggc aatggactgg
    36721 atattatcag ggttttcgat gcgttgaacg accttaggaa tatggagata gcggcggatc
    36781 aggtcaaaaa ggaaggaagc agtcttcaga tgtgcatctc ctacaccata tctccagaac
    36841 atacgctcaa aaactttgcg gacctcgcgc taaaaatgaa aggtatgggg gcggattcga
    36901 tatgcataaa agatatggct ggattgctcg gtcctatgga tgcgttcaaa ctcgtagggg
    36961 cgataaagga aaaaacatct ctccccgttc aggttcactc ccattacacc agcggtatgg
    37021 cggccatggc ctattatgct tccatccagg cgggagcgga tgtggtagat tgctccatat
    37081 ctcctctggc aatggggaca agccagccag cgacagaaac catggtggcg gctctttcag
    37141 gcggggaatt cgatacaggg atcaaactcg agaaactgat acccgtctcg gagcatttca
    37201 ggagagtcag ggagaagtac agcagccttg tgatggggct tgagggagtg gatatcaacg
    37261 tcctgatcta tcagataccc gggggtatgt actccaacct tgtgagccag ctgaatgagc
    37321 aaaactcact cgacaaactc caggacgttc tcagggaagt accgaaggtc aggaaggaaa
    37381 tggggtatcc gccacttgtt acccctacaa gccagcttgt tggtacccag gccaccctca
    37441 acgttcttac cggtgaacga tggaagatag tttccaaaga ggtaaggaat tatttccttg
    37501 gttattatgg aactcctccg gggaaggtcg atccggatac ccgcaagaaa gtaattggcg
    37561 acgataaacc tatcacatgc aggcccggag aagttctgga gccggaactc cccggggtgt
    37621 acaaatccat gggggcctgg ataacccagc cggaagatgc cctcagctat gctcttttcc
    37681 ccgctgtggc aaaagatttc cttgtatcga agtttgccag ggagaacctg cgggatgtcg
    37741 ggttggagcc gcttgttgat gatgccggat accctctgta gaccgctttt gaacgacact
    37801 aagatcgccc ggttttttgc tgatatgcag ggagtgaatg atcgttatga tcgaacctga
    37861 gattaacagg gggccaattg caagtggcgt ttcgcaaaat gctatagagg ctcccggtat
    37921 cgaggttctt gcgagactcc tgggtccggg agacgagaac ctgaggcttt tcgaggagcg
    37981 ttacccggta aggatagccg tgaaggatct ttcgctggtt gtccaggggc ctgatgcctc
    38041 tgtggtagtc atggttgaag agctgctttc gcaacttctt ggagtggcac gcaggggaca
    38101 cgttcttaga cccgcagagg tcacctatgc catgaacgta tttgccgaag aaggggttgt
    38161 ggacctgctt cccatgtatg aagatgtgat ctgcatgacc tacaggggta aacctgtccg
    38221 gccgaggacg agtggccaga agcgatacgt agacgcgata aggaagaacg atgtgctttt
    38281 tgcgataggt cctgcaggga ctggcaagac atatctggcc gtcgggcttg ctgtagctgc
    38341 gctcaaggag ggaaaggcca gcagggttgt ccttgtccgt cccgcagttg aggctggaga
    38401 aagcctgggt tttttgcccg gagacctgag agaaaaagtt gagccctacc tgaggcctct
    38461 ctatgatgca ttctacgaga tgttctcccc ggagcgtttc atgaaatacg tcgaaaaaaa
    38521 agttatcgag atagctcccc ttgcctacat gaggggaagg accctgaacg acagttttat
    38581 catacttgat gaggctcaga acactacgcc ggagcagatg aaaatgttcc ttaccaggct
    38641 gggtttcggt tctaaagctg ttgtgatagg tgatgtcacc cagatagatc ttccttctgg
    38701 aaaggattcc ggactcaagg tagtaggcaa tattctcagg gacatttctg gaataggctt
    38761 tatatccctg gacaagaccg atgtcgtcag gcacgaggtt gtccagagaa tagtccaggc
    38821 ctacgaggaa tatgagcaga agaagcagtg aaggcccact cgttataagc ctcgcgaaga
    38881 tgatccgcaa actggcagcg gaccccgaat accgcttcag atggctagct tccattgtcg
    38941 ttgtatctgc ggctgtcctg atgttggcag cctcgtggtg gcttagcgag ggcggcggtt
    39001 cattccgtca gggagcgccg gcaccacgga cctattttgc ccactccaga atgaagatcg
    39061 ttgacgagaa cgccacaagg gctcttcggg aacagaggat agccgaaatt gtgggcgtcg
    39121 ttgtcaaaga cagggctcat ggagaagagg ccgaggggat gctcgttctt ctcgaaaggg
    39181 gcgaatacgg ggaggccctt ccagactccc ttgcagtgat actggaagcc cttggagaag
    39241 attcacgaaa tcgggttatt tcggtttccg tgatgatcgc tgaagagttg atcgggtcac
    39301 ctgaaatggc ccttgacgat gatgacccca tatggcagaa actcagttta tcgccccttt
    39361 cagttccaga acagaatctc gctttccaga ttcttgatca tatcctggga tcttctttca
    39421 aggaagatcc ccaggctacc cttaaattca agcaacaggt cgcatctgaa gttgttcccg
    39481 tcgagaggat cataggagtc ggagagacta tagcccgtga aggccagttg atctcgcctg
    39541 aaatggcaat tatcctgagg gcccagggat accccgagag agagatacca tggaagactc
    39601 ttttattcgt ctttctggcg acgactctat ggatattctg gatgtggtgg tacaccggga
    39661 gaagggattt ttcttttgac aggcgggaat ggctcttcat aatctttatc ctgataacag
    39721 gatggctggc aatgtttgtt tcctcacggt ttgcggggaa cgtcctcgga ataattgctc
    39781 ttgcaggttg ggcctacctg ggacttcccg gatcattttc gttccatctc cttatatggg
    39841 gcggcgttat tggttctgta atagcctccg ggagttcatc catggacctg atgctctccg
    39901 tttttgccac cgtattaact gccagccttg ggtatgtcct gatgaccagg atcgacagcc
    39961 gctggtgcct gataagaaag ctcttgcttc ttggtctggg gctttccttc ggtgtttacg
    40021 tgatcatgtg gggcctttac ctgccctccg gctggaagat acttgttacg tatgtttttt
    40081 ccgctgtcgt gtgggttgct gttactatcg gggccctccc gctatgggaa aaattctttg
    40141 atatcctcac tcctataagg cttgtagacc ttacccatcc gtctcaccct cttctcaaaa
    40201 gacttcagat agaggctcca ggaacctacc accattcaat aatggttggg acccttgcgg
    40261 aagcggcggc ccaggggttg aaaatgaacg ctcttcttgt aagggcaggg gcatctttcc
    40321 atgatgttgg gaaactcaag cgcccacagt tctttgtgga gaaccagcag acaggacata
    40381 atcctcacga tgaactttcg ccaaccctgt cggcactgat cataatttcc catgtaaggg
    40441 agggtctcga aatagccagg caatacggga tcccccagaa gataagggtt tttataagcg
    40501 aacatcatgg aaccacctgt ctgggatact ttttcaagaa ggcgtccata acagaaccgg
    40561 atctgcagat ggatcagttc tgttatcctg gaaaccgacc cgcaagcaga gagtcggcag
    40621 tggtgatgct ggcggattca gtggaagccg cggtcagggc ggccgcaggt gacatcagaa
    40681 atccgaagga actggaggag gtggtcagca atgtcattga aacaaagata tccgagtccc
    40741 agcttgacct tgtccccctg acccttcagg atattaccag aataaagata gctttctcgg
    40801 aaacccttaa acacatgtac cacaccagaa gggtttcccc tgtatcggaa gaaacgaaga
    40861 cggaggaaga tgcgtgaagg tccatatcga ttacgaaccg ggaagtttca gcaagcgctt
    40921 gaaaccggaa ctggtctcag gattgcgaaa ggttcttgac cctgtgacca gagaacttgt
    40981 cctttccgtt tcagaagaaa atgtccttcc ggatgaggcg aggttctcga tctttttggt
    41041 cgatgaagag gagataaggg cactgaatct tgaatatctg ggggaagact cgcccaccga
    41101 tgtgctttcg ttccctgttt tcgactttga acatgatgga gttttccatt cggatgcccc
    41161 cctggtactt ctgggcgata tagttatctg ccctccgcag gtagcggcga atgccagggt
    41221 agctgggcat tcattcgcga gggaactttc cctggtcctg atccatggta ttcttcatat
    41281 cttcggtatg gatcacgaca ccgaggaaaa aagagaagca atgtggaaaa aacaggagca
    41341 gtattgggat aaaattgagg aagccctgta tcccttggaa tgggacaggt aatatatgaa
    41401 agggttgata cgtaagtgag cgaagaaatt gcccaaagta tcctcatact ggctttcctg
    41461 ctgggtctgt ctgctctctt cagcagtagt gagacggcga taacgtccat cgggagggga
    41521 aaactccttg ccattcagga aaaaaatccc cagaaaaagc ggggtataga gtggctgatc
    41581 tcggatatgc atagagccct gacggttatt ctgataggca acaacctggt caacattgcc
    41641 gcaagcgccg tggcaacctc gctggccctt tccctttttg gacccaaggg cctcctcgtt
    41701 gcggtggctt tcatgaccgt aatgatagtg gtatttgggg agatcctgcc aaaaagcatg
    41761 gccataataa ggccagaaaa gattgtgaca gctacccttc ccctgctccg tttcataaac
    41821 gttatctttg ccccgctgat atgggccacc gtagtgacag tcaagttcct tggctccctc
    41881 atgaaggtgg acctttcctc acagcaccct tttgtaacca gggaagagat agaacagatg
    41941 gtgaacatag gggaggcctc cggggttttc gaggaagatg agcgcagaat gatccacgga
    42001 gttatctcat ttgaggagac gagggtatac gagatcatgg tgcctagaac cgatatggtt
    42061 acagtatcgg gtgagatcag tgtccaggaa tcggtatcgg tattccggga atgcggtcat
    42121 tccagacttc ctgtctatga cagggatccc gatcatatac ttggcattct ttacgtaaag
    42181 gatctcatcg ggtctcttat ttcgggagaa accgccgttc cggtatcttc tctggtaagg
    42241 gatgctcttt ttgtcccgga aacgatgcgc atcgccgatg tgttcgatgt gatgaagggc
    42301 aagagagttc atatcgctat tgttgttgac gagtacggtg gtactggcgg cctggtgacc
    42361 ctagaggatc tcattgagga gattgttgga gagatacagg atgaatacga tgatgaaagc
    42421 gcattgatcc agaaagagga cgatggaagc tacctcgtaa agggacatat gggtctcgag
    42481 gatctgagcg agatgttgca ctaccccttt gagtccagtg acatggatag tgttggcggc
    42541 ctgatccttt ccctggctgg gagattccct gacgagggac aaagaatccc ttacggtcac
    42601 tgggagttcc aggttcttga ggtggaggat caccggataa aactggtgaa aatcactcca
    42661 atggacgaca tgtaccgcat ctgtgaggtg gttgaatgaa ccagggatac gactggaagg
    42721 ctggcggagt aacagcggac atgctccttg aagaggccag gagagttcag aggttctcct
    42781 attctccgta ctccggtttt cccgtggggg ctgctgtggt agctgccgat ggcacgattt
    42841 tttgcggctg taatgttgag aatggaagtt acggcctgac aatttgcgcc gagaggaatg
    42901 ccatgaccag aatggtgtct tcgggtggca aggatcccgt cgcaatagca ataacgggtc
    42961 ctcctggagt cccgtgtcct ccctgtggag cttgccgtca gtttctagcc gagttcaaca
    43021 ggaggttgct gatcgtgctc gaagcaccgg aaactggaca cgtgatattg tcccttgagg
    43081 atattttccc catgcccttc tctttcggag acagaggctg aatatgtcgg atgccggatc
    43141 aaggtcgggg tttgtttccg taatcggcag gcccaacgtt ggaaaatcga ccctcgtcaa
    43201 tgccatagtc ggcggcaagg cggcgattgt atccgacaaa ccgcagacca ccagaaacag
    43261 gataagagct gtttacaacg acccaagggg acagatagtc tttgtagata cccccggcct
    43321 tcacaggccc ctgcacgaat tggggcgctt catggaacgc caggcatcgg agtctcttgg
    43381 ttcatccgac gcagtctgct atgttatcga ggcttcggat acccatatcg ggaaggctga
    43441 tgaaaaaatt ctgaagatca tcgaaatgtc gtctgtaccc gtcgttcttg tcgtaaacaa
    43501 gacagattat gactctccgg ccagggataa aaaaggctgg aaggtatttc tggaaaggtt
    43561 aagttccgcg gacgttgttg aagtttccgc actcaaagag accaatatgg atcttctttt
    43621 agagaagatt ttttctcttc ttgtgcccgg tccaccgatc tttgacgacg accggatggt
    43681 tgactgcacc gagagattcc tggcttccga gattatcagg gagcaggttc tgatgcagac
    43741 agaacaggag gttccacaca gtgtcgctgt agatgtggag gagtttcagt cacccgagga
    43801 gtatcccgag agaaagaacc tactggtaag ggccagcatc tacgttgaga gaagcggcca
    43861 gaaagcgatc ctgatcggaa agggcgggac caaactaaaa aacataggcc ggaaggcgag
    43921 gctggaacta cagcagcagt tcggtcatga tgtctatctt gatctctggg taaaggtcag
    43981 gccaggctgg agaaattccg ggaacgaact tagaaggatg ggatatacct agggagactg
    44041 gtcgtggaaa acaaatccgg gcagggtatt ctgaaaaacc gcggagtggt tctgcgaagg
    44101 agcgatacac ccgagggaaa cagaagtgtt tatgttctcc tggaaaacgt aggccccgtc
    44161 tggcttctgg ccccgggggc ggccaggggt aaagtccggt tcgggggatc aaccgatccc
    44221 cttgtctggg ggacatttca cgtctacagg ggtcctaacc gatggtatct tcgggatgtt
    44281 gacgtgagga aggacttctg gtctttgcgg tcttcatcca ttagaatacg gcaagctgtc
    44341 cattgggtgg gttcgatact cgggaacacc cttccaggac acccgtgcaa cgatcttctt
    44401 cctgtttttt tctggtccct gtcgttgctc gagaaccctt ctgcaagaga ggaccttgcc
    44461 agttggcgtt tctactggcg ctggctcagg atatggggaa tggctccgga actggaaaga
    44521 tgccgttcgt gcggatcact ccccaggaga gcggtctttt ctggcgaagg cattaattgc
    44581 gaaaaatgcg gaggacaggg tatacccctt tccgagcagg aacgcctggc tcttctcgaa
    44641 gcggcaatat caggtcacag ggattttatt gaaagatcgg ggcaaccagg gataggatcc
    44701 cgcctggtgg aaaagtgtac cctggttctg gagagagaac tggctgaaag aacgtgatcg
    44761 aaatgtcaaa ggaggaatat ccgggtgaat ctacaggaga taatcatgcg cctcgagcgt
    44821 tactgggcag agcaagggtg cattatccag cagccttatg atatcgaggt aggagcggga
    44881 accatgaacc ccgcgaccac tctaagggta cttggcccgg agccgtggag ggttgcctac
    44941 gtggaaccgt cgagacgtcc tacggatgga aggtacggag agaaccccaa caggcttcag
    45001 cattattacc agttccaggt tatcatccaa cccgcccccg aggagattca ggaaatgtat
    45061 ctggagagtc tcaagacact gggtatcgat cccggggacc atgatatccg ttttgttgag
    45121 gatgactggg agtctccaac cattggggca tggggacttg gatgggaagt atggctcgac
    45181 gggatggaga taacccagtt cacctatttc cagcaggttg gcggtattga catggacccc
    45241 gttgctgccg aaataaccta tgggatagag cgtatcgcca tgttcgttca gaaagtcgac
    45301 aatgtctacg atctggaatg ggtgggcaag gtaaaatacg gagatgtcca tcacagggga
    45361 gaggtggaac attccaccta caactttgag attgccgata cagaaatgct gttccggctc
    45421 ttctcaatgt acgagacgga agcaaaaaac atccttgaaa aaggtctggt atttccgtcc
    45481 tatgactacg ttctgaaatg ctcccatgcg ttcaacctgc tcgatgccag gaacgcaata
    45541 agcgtgaccg agagaacggg atatatttcc agagtccggg ccctggccag cggatgttgt
    45601 caggcttacg caaagcagcg caaggatatg ggttatccgc tgatgaatag tttcgataca
    45661 gagaacggga ggtaggagta tgggaaaaag ggatcttctt cttgaaatag ggaccgagga
    45721 aataccctcc agattcatac ctccgagcct ttcggaactt tcccggatcg caggatcgga
    45781 gatgaagacc gagcggctat ccttcgataa tatccagacc tttggaacgc caagaagaat
    45841 ggcgttcatt gtaagagacc ttgcagaaag acaggaagac ctcattgagg agttcaaggg
    45901 cccggcatgg tcctcggctt ttgatggcag cggaaacccc acaaaagcgg caaggggttt
    45961 tgctaaaagc agaggcgtgg aggtcgactc cctcgaaaaa aggaccttcg gtgaaaacga
    46021 ttttgccgtt gccgtgatac gtcaggaagg aagaccggtc attgaaatat tgccgggact
    46081 tcttgagcgg attacaggcg ggatatcatt tcccaaaagc atgtactggg acaggacctt
    46141 caccaggttt gcccggccgg taaggtggct tttggccctt tacggagata ccccgataaa
    46201 cttcggtttc gggtctgtca aatctggcaa aaccacccgg ggtcaccggt ttatggggaa
    46261 aaagatcata gagatagaga acacggcaga atacctggaa aaactccacg acaactttgt
    46321 tattgccgat ccggagaaac gaaaagaaaa gatggtttct gggatcgctg gcctggaaaa
    46381 ggagatcggc ggaaaagcag tgctggatga agacctcatg gaggagaacc ttttccttgt
    46441 ggagtatcct gtacctttct tcggatcctt cgatcaatcc ttcctcgaca ttccagccga
    46501 agtgcttata acgtcaatga agaaccacca gcggtatttt ccggtgaggg atacatcagg
    46561 cgcgctaaag ccattctttg tcggtgtcag caacaacagg gctaccaata tgtcagtggt
    46621 cagggagggc aacgaaaggg ttctcagggc caggctctcc gatgctgcgt tcttctggaa
    46681 ggaggaccag aaggttgcac tctccgcaag ggttgaatca ttgaaaaaga tcattcatca
    46741 cgagaaactg ggtactgttt acgaaaaggt ctcccttgtc cgtgaactct gttcctttgt
    46801 tagcgaaact ctctctctct ctcgagaaga gacggaactg gtcgacaggg cggcgatgct
    46861 ttccagggcg gacactgcca ccgaaatggt ctacgaattc cctgaactga gaggtgtaat
    46921 gggtagggag tacgcactta aaggcggaga ggacccaagg gtggccttgg ctctttacga
    46981 acagtatttg ccggcttttt ccggtgatga tgttccgaaa gacaaaatag ggggtattct
    47041 ggggatctgt gacaggatcg acacgatcac tggaggattc aaagccgggc tacagcctac
    47101 cggatctcag gatccctatg gtatccgcag ggcttcaagg accctcaatg aaatattgtg
    47161 gggcagcagg attgatgtag acattctcca gattcttgcc gaatccgcca ggcagagggg
    47221 cctttcagag gccgaaactc tcacagcaag ggaatttatc cttcagaggc ttcataacca
    47281 gttgagagag aaaggcttct ctcacgaact tacgaccctt gctgtgtcgg tggctggatc
    47341 cagaccaatg caggcaatga ggatgctgga tgttttctcc ggaattcagg attcggaatg
    47401 gttttcggga ctggtggttt ccgctgtcag ggttaaaaat attctccaga aggcgcagga
    47461 gcgaaacatg aacctggaca gcggactcct ggtcgaaaaa gaggaaaaag aacttttcga
    47521 gatcgtcgag actctctctc ctgatgtggc cagtgccgtt gctggatccg actgggatgg
    47581 tctggcaagg ctccttgctc gactcgaacc cttcataacc tctttctttg accatgttct
    47641 ggttatggac aaggatgaaa agatccgaag caacaggatc gccctgttgg aaaaatgcaa
    47701 tgaccttttc aggactgccg gagatctggg agttctgaaa tcctgagacc atgaactaca
    47761 attttcccgg gacaggagga ccttcctgtg aatgacccag aaagtaaagc gccgctccca
    47821 gtatttgtcg tctcggattt caccggtgag accgccgagc acgtggccaa ggctgcgatg
    47881 agccagtttg ctacggacgc tgtaacactt cagagataca ggtacatcaa taatcaggag
    47941 agagcactcg aggtgctcga agaagccggt tccataaacg ccctggttgt gtgtactctt
    48001 gttgaccatg acctgaggcg atggttcatg gaaaaggctc atctccagaa gacggatacc
    48061 gtcgatataa tggggccaat gctggacatg cttgagatgc gtctcgaaca tcctccgctt
    48121 gagaccccag gccttatgag aaggatggac gaagaatact tccgcagggt aaaagcaata
    48181 gagttcgcca ttaagtgtga tgacggaaga tgcagcgaac agctcctttc cgcggatgtg
    48241 ctcattttgg gtgtttccag gaccagcaag acgccccttt gtatgtacct tgctcacagg
    48301 ggtttcatgg ttggcaatgt gccgcttatc ccggagactg aaacgccgaa ggaaatattc
    48361 gatgtagctc ccgagaaggt gttcgggctg gtggtccagc cagagaagct tgtcgaaata
    48421 cgcagggaaa gacttcgcct tctgggtctg gattccgata cttccaccta tgcccagtgg
    48481 gaaagggtcg aagaggaact gaagttcgca aaggatatca tgaggaagat cggctgccga
    48541 atactgaatg taaccaacag ggcaatagag gaaaccgcac aggaaattct ggattatttg
    48601 agagcctgat ccatcgaaga aaaaacggag gaggtcgaaa gataatgaca gaagccggaa
    48661 aaacagaaaa gcgttttgtt ttcaacttca gcgaaggcag ggccgaccag aaaaaactgc
    48721 tggggggcaa gggtgccaat ctcgcggaaa tgatgcgaat cggtcttccg gtgcctccag
    48781 ggttcactat ttcaaccgaa gcgtgtctgg aatatatcag taaaggagag ccctttcttg
    48841 atggactctg ggagcatgtg ctttcttaca taaatggact ggagaaggag acaggcaaaa
    48901 ccttcggcgg agaaaagtca cctcttctgg tttcagtcag gtcgggcgct gccatttcca
    48961 tgccgggaat gatggacacg atccttaacc tggggcttaa cgagaccact gtcaaggctc
    49021 tcgccgccga atccggagac gagagattcg cccttgacag ttacagaagg ttcatccaga
    49081 tgttctctga tgtggtgctc ggaatagatg gtgacctttt cgagaacgtt atccacagtg
    49141 ccaagaagga tcttggactc tcctacgact acgagatacc agcctcttcc tggaaagata
    49201 ttatcggaaa attcctcagg atcgtggaac aggaatcaag gggacctttc ccctccgagc
    49261 ccatggtcca gttaaggaaa tccatcgaag ctgttttcag gagctgggat aacccaagag
    49321 caaaaaccta cagaaagatc aacaaaatac ccgatgatct cgggacagcc gtaaacataa
    49381 tgaccatggt attcggaaac cttgggaatg actgtggtac tggagtatgt ttcaccagga
    49441 acccttccaa cggagaaaag aaactctacg gggaattcct gataaatgcc cagggagagg
    49501 atgtagtcgc gggcaccagg acacctcagc ccatcgattt tctgggcgag aagttgcccg
    49561 aggcaagcag tgagcttcac agggtttccg aactcctcga aaaacattac aaagatgttc
    49621 aggacataga gttcactgtc gaaagaggaa aactttttat gcttcagacc cggaatgcca
    49681 agcgaacatc gagggcggcc gttcaggtgg ctgtcgatat gcatgccgaa gggctgatcg
    49741 atgccactac ggcagtatcc agggttactc ctgaccaggt ggaacaattg ctgcacaagc
    49801 agatagaccc cgaggccagc tttgatattg cggccaaggg ccttcccgca tcccctggtg
    49861 ccgccactgg catagtcatt ttcgatgccg atgaagccga gaaggtttcc aacagcgggc
    49921 agccggtgat acttgtcaga ccggagacaa cacctgatga cattcatggc ctttacgcgg
    49981 cccagggtgt ccttaccagc aggggaggga tgacaagcca cgccgccgtt gtggcaaggg
    50041 gccttgggaa gccatgtgtc tccggatgtg agtcggcagt tatcgacgtc aacagggggg
    50101 agatgcagat cggggggaag acggttcgaa aaggggagac aattacgatc gatggtacca
    50161 gggggcacgt cattacagga gtggttcccc tcgtccagcc gtccttcact gatgatttca
    50221 gacaactgct tgatatggcg gactccatct ccgatcttca ggtttgggca aactctgata
    50281 ctccagagga ttcggagaga gccagatcct tcggtgccag gggtataggc ctttgccgta
    50341 cagagcatat gtttatggcg acggaaagac ttcccgtcat gcaggagatg atccttgctt
    50401 ccgacaagga ttcccgggta gaggctcttg aaaaactcaa gatcatgcag agagacgatt
    50461 tccggggaat attcgaggcc atggaaggac ttcctgtcac cgtaaggctc cttgacccgc
    50521 ctcttcatga atttctcccc aaggaagaag aactcaagga aaccatcgct tccctgaagg
    50581 gtcagtcagg taaagaactg gaaaaggcag aggcaatatt gaaaagagtt ctctctctca
    50641 aggagaacaa tcctatgctg ggattcaggg gatgcaggct gggactcgtt tacccggaga
    50701 tctatgaaat gcaggtttgc gcgataattg aggctgcctg tgaactttca cttgcgggta
    50761 agaaagtaat ccctgacatt atggtccccc ttgtgggaac aaaagaagag atgaagaggc
    50821 tcgaccggat ggtaagagat acagccgaac aggtgataaa gaataagggg cttgaaaagc
    50881 ttcactacct tgttggaaca atgatcgaag taccgcgggc tgccctggtg gcaggggaga
    50941 tagcggagtt cgcggagttc ttcagtttcg gaaccaacga tcttacgcag actactttcg
    51001 gctactccag ggatgatgcc gaggggaaat ttctcacccg atatgtggag gacggagtcc
    51061 ttaaggacaa ccccttccat gtcctggaca gggacggtgt gggacgtctt atgaagatcg
    51121 ctgccaaaga gggcagggag gcccggccgg gtatccagat aggcatatgt ggggaacatg
    51181 gcggtaaccc ggaaagtatt tccttctgtc agggagcggg tctgaactac gtgagttgtt
    51241 ctcccttcag ggttccggtg gcgaggctgt ccgcggcaca tgccaaactt ggattgctgg
    51301 actagatagt tcgagggccg cccttttcgg ggcggccttt tttaaaggag aaattatgac
    51361 tgcagagata cctcgtataa gatgggaaaa attagaaagg actattcttg ctccctttgc
    51421 cgctttcagt gactccagtg gagggagggc caggacagag attccctgcc cgataaggac
    51481 cctgtatcag caggacaggg acaggattat tcattgcaag gcgttcagga ggttaaagca
    51541 caagacccag gttcttctcc ttcccgaggg ggatcactac agaaccaggc taactcatac
    51601 cctggaggtt tgccagatag ccaggaccat atccagggct cttgccctta atgaagacct
    51661 gacagatgct attgctcttg gacatgacct gggacacact ccctttggac acatgggtga
    51721 acgtgctctg gacaaaatcg cccgcgagaa aggcctggat gggtttgccc atgccagcca
    51781 gagtctcagg gtggtggacc acctcgaaaa agaggggaaa gggctgaacc tgaccttgga
    51841 ggtcagggac ggtattctga aacactcgaa agggcaggtc gacgtaagag agggtttcac
    51901 cggcggagaa acaactctta cactggaagc tcgtgtcgta agggtttcgg attccatagc
    51961 ttacatcaac catgatctgg acgatgccat gagagcaggc ctggtcaaag aatcagatcc
    52021 tcctgctgat gcgatagggg ttctgggcaa gactcatggc gacaggatag gaatgatggt
    52081 taaagatacc gtaagcaaca gcgggccagg aggagtaaca atgagtccgg aaattcttga
    52141 ggcagtcgaa aaaatgaggt cttttcttta ttccaacgtc tatgcctctg aaagggttct
    52201 tcgggaagaa cccaaggttg agcacgttct cagaactctt ttcgaccatt acgaaaaaaa
    52261 ccccgtggga acgatcgctc ccgacggggc tcctccctgt caaatggcgc tcgaccatgt
    52321 ttcgggcatg acggacagat atgcgctgtc tctctttgag aagatcgcga tgcccgatcc
    52381 gtggccccag atcgggcgct aatcttcctc gatcccgtac tgctttagca ggtcctggta
    52441 tctttcctct atctcttcgg gtgtccgctc cttcttcaca taaggctctg gctgtgtatc
    52501 cgcctgaccc ggttttgaaa ccgggggtgc ataggtagtg gccggctgtt gggatgatat
    52561 ttcatccatt ggtccgtaat aggcggtgtc tgaaatagaa acaccgtata gtctcgccat
    52621 ggaggcagtt atacctgtga cttcttcgtc cctgggcgaa agaagcatgc gaggaacatt
    52681 ggggtcactg gccgccatgg cccatgaccc gccatgaaga ggacatatgc tggatggggc
    52741 catcccgtcg ggaaggatga taacggaagc ggtgcaacca cctgaaacca ggaaaccagt
    52801 atccttgcat acggtgacga tctggacatt tgcagattcc ggtaacgtga aagactccct
    52861 cacgggtagg tatttcacgg cctcgctcat gaaagcctgc catactggag cagcaatgac
    52921 acctccggtt ccgggtctgt cgaggctttt gtggtcgtcg ttcccggcgt agactacagt
    52981 taccagacca ggtatgcccc cggcaaacca tgcgtcactg tattcgttgg tggtaccggt
    53041 tttcccgaag acttcataac ccgggatcct ggccctggtg ccagtgccgg agcgaacgac
    53101 ctccatcaat acggaacgga cagagacagc tatctcttcc gggatagttt tggtcaactg
    53161 aggaccgtat tcctccagaa tttctccggc tcctgtaacg accctccgaa tcgcgaaggg
    53221 ggtgaccttt gaaccgtcgt ttgcgaatat gctgtatgcc gcagccatct cgagaggggt
    53281 tacgcttgcg gaaccgaggg ccagagacag gtcggctggg agatgaggag atgtgaagcc
    53341 tgccttacgg gaaacatcga ggatgttttc aactcccgtg atgtgagcga cccttaccgc
    53401 gactgtattg atcgagtgaa caagggcagt cagcagcgta acctcacccc tgaactcccg
    53461 ggtatagttc cctggttccc agatgtttct ctttccctgg tcgtctatgc cgttgtcata
    53521 ggatacggga gcgtcaaggg cgtgatccac gggcctcagc cctctcatca gggcggcggt
    53581 gtagacaaaa ggcttgaagg aagaaccggg ctggcggaat gcctgtgtgg ccctgttgaa
    53641 cttgctaacc tcgaaatcct ttccccccac catggcaagt atctcaccgg attcagggtc
    53701 gagtgcgaca attgctcctt cgctcttaag tccctgtatt gccttctcgg cagcgtgctg
    53761 gacatccaga tcgatggtcg tgtgaaccct gaggccaccc cggtagacta catcgcttcc
    53821 ataggtcgga agaagatgtt cgaacaaaat gtgagagatg aaatagggag cctcgtcgaa
    53881 cataagggca ggttttttca gagaatggaa atccagttcg atcttctggg cgtcctgggc
    53941 ctcttctgga gtgatcattc ccaactgcac cattctgctc aggacatagg tctgtctgga
    54001 agcagcgcgg tccggatggc gtatgggagt gtaatactcc ggtgccttta caaggccggc
    54061 aaggagagat gtttccgata gagtcagttc cgagggctcc ttattgaaat acagctgtga
    54121 ggcggaatgg ataccccagg cgccatgccc gaaatagatc atgttgaggt acatctccag
    54181 gatctggtcc ttggaataga tcctctccag tcgaagagag ataatagcct ccttgatttt
    54241 tcgggtcagg gtctgttctc tggaaagaaa aagatttctt gcaagttgct gggtgattgt
    54301 gctcgctccc tgttttgtcc tcaggtttgt aacgttgacc cagaaggctc tcaatatacc
    54361 cgaaaaatca agtcccccat gggagtagaa agagctatcc tctgccgcaa cagcagatct
    54421 ctgcatgttc cttgagatct gggaaagggg gacccatgtt ctgttttcca gaaaaagacg
    54481 ggtaataacc ttctggttcc tgtcatagac tattgttgag agatcgggtt cgtagtttga
    54541 catctcctcg agagatggga gagaagaagc ggcgtttctt attacgatcg caccgacaac
    54601 cgaggccagt gctattgcag ccataaaaac cacaagaact gccagaagag cgccacgcac
    54661 attattcccc ttcggttttt tgccccctgt ttcaacggtt tcgggatggt ccgttttatt
    54721 cctgttgttt ttaccgaaaa attttgaaaa acctgttttc attgatattc ctctcttttc
    54781 aggaaagtca gggaaagttg ccgatatcaa gccatagaag aataacagaa aaaggggaaa
    54841 ttacgaatac caaaaataat aatttacaaa taaacccttg cgttcagcac ggatgcgtgg
    54901 tatatttttc gccgtcgtct gaacaagacg gcggcacctt gaaaaaagaa tacagcgcat
    54961 atggatagag cgggcgaaga aaaacgccag aaatattttg gagagtttga tcctggctca
    55021 ggacgaacgc tggcggcgtg cttaacacat
//
LOCUS       DAOTIQ010000005        48750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_744_length_48750_cov_7.374125, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000005 DAOTIQ010000000
VERSION     DAOTIQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 48750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48750
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_48750_cov_7.374125"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="P5541_01575"
     CDS             complement(<1..373)
                     /locus_tag="P5541_01575"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HRV70693.1"
                     /translation="MKGRARHAKVFLAMMQILDQKAKPEARLLDLSANSRMADEQYAL
                     TRGKYEKITRKYFREDGTLLPFTLKARERVALPRKIVEPFGQGVRYSKKELNTLLAKI
                     AEDYVLVRRCLVDHGFVSCRAD"
     gene            987..2678
                     /gene="ilvD"
                     /locus_tag="P5541_01580"
     CDS             987..2678
                     /gene="ilvD"
                     /locus_tag="P5541_01580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV70694.1"
                     /translation="MTWRSKRLGLFSGRGSASRRAIYKGCGYDDEDLSRPLIGVVNTA
                     NDAGLGHVHLDRLAARVKAGILQAGGTPFEFSTIATCGAVPIGTPHLRYELVIRDVIA
                     SSVEIMANVQLLDGLVLLASCDSIIPGVLIGAIRSGLPSIFLSGGPQLVTRFEGRQAV
                     MSELDQLVFGAQYGDEDVSAKLRYLEDNVCPGPGACALMGTANTMQILAEGIGMSLPG
                     SATVPAVYAEKERFATRTGRRIVDMVKEDLTPRKIISRENLLNGITLTMGIAGSTNAV
                     LHLLSLARDLDIDLDLDDFDRISREVPVISRVIPTGKATVVDLHHAGGVPAIMGELGE
                     YLHGGALTVSGLRAEDIFSKERSKDLSVLTTADKPVLANGGIAVVRGNLAPDGAICRT
                     STISEKALRFKGPARVFDSDEEAHSAVVEGKISKGDVVVIRREGPRGAPGMREMMMTT
                     DALVGLEMGQEVFVVTDGRFSGFTEGAAVGHLSPEADVGGPIAVVREGDPISIDIPAR
                     RVDLDIPAEELKRRLSEWIPPQPKVSRGILGLCAKTALQAHLGAMLEDRVSNPKA"
     gene            2775..3146
                     /locus_tag="P5541_01585"
     CDS             2775..3146
                     /locus_tag="P5541_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRV70695.1"
                     /translation="MKQEIRTDRSPDPIGPYSQGLKVGNRIYVSGQGPLNHETGKTPE
                     TIEEQTRQVLTNIRNILEAGGATMDDVVKTTVHLADLKDFQAFNSVYKEFFSPPFPVR
                     TTVGSQLLGILVEIDVIAEIQ"
     gene            3265..3969
                     /locus_tag="P5541_01590"
     CDS             3265..3969
                     /locus_tag="P5541_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV70696.1"
                     /translation="MERILLADDDTGLCELLTEFLESEGFKVESIHDGRKVASRVLAE
                     RFSLIILDVMLPGLSGMEILKEIRRQSAVPVLMLTARGDDVDRIVGLELGADDYLPKP
                     FNPRELVARIRAVLRRGRMDSSRSSQTGTYHVGDMTIDPAARYVSAGGKRIELTTVEF
                     DLLETLAHSAGTAISREVLVRSVLKRSFSPFDRSLDVHISNLRRKIGTFSDGSDRIKT
                     VRGLGYILALPTEESG"
     gene            3969..5384
                     /locus_tag="P5541_01595"
     CDS             3969..5384
                     /locus_tag="P5541_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV70697.1"
                     /translation="MRERKLFQRIFLWFLGAMLLMVLVSVLLTVFMTQQGIILTGQQE
                     ALSNALDRNGKGLLEVVETGNPARLRESIDRIRSETGLGIFIFDVDGVPVPVEGGPSK
                     DFLERLGRDAADLLGQGTTFQTRGEFSSVFRQLGSPSGKDYILVGVFRRSPYIARVLT
                     GNPKALLIHLMGLLATALVFCFLLARYLSDPLVRLSKTARSVSSGQLDTPVEDLVKDR
                     SDEIGELARSFEAMTKHLTTLLEAQRRLIRDISHELRTPLARLGVALELARKRSGTVA
                     RESLERIERESEVLNDMIGDLLSLSKVEAEMDHVTFEMVDLAAILENVVEDANFEARV
                     IKRRVELVYPDDLEPIRLNSELVRRAVENIVRNGLRYTPEGTAVSVKAERRSRGGRPG
                     VSLTVLDKGPGVPEDELQQIFKPFYRTETSRSRDTGGSGLGLAIAQKAALAHGGEINA
                     ELPSGGGLLVEMWLPLQSMDL"
     gene            5477..6394
                     /locus_tag="P5541_01600"
     CDS             5477..6394
                     /locus_tag="P5541_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRV70698.1"
                     /translation="MDDHRMTAPVAAALVSVYFFWGTTYLAMKFAIETLPPFIMLGLR
                     FTTAGAIMFVWEWIRLRPRISWGQWRGMAMVGALMLLGGTGGVAWSEQVVPSNIAAIV
                     VATVPLWIAIIRWVYLRHDRPARITIIGLVTGFGGIIMLVASTSGPSAAEGHITGLVV
                     LGFATFLWASGSLYSRVADQPDSPALAISMQMLTGGLMNLVVGIAWNGGSLVPMSEVS
                     IRSATALGYLVVFGSIVGFSSYIWLLKKTDPALASSYAYVNPVVAIILGWVLAGESLN
                     AGSLAATAVILLGVVMIVRGTVRPAIRSS"
     assembly_gap    6537..6636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6637..7744)
                     /locus_tag="P5541_01605"
     CDS             complement(<6637..7744)
                     /locus_tag="P5541_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70699.1"
                     /translation="MKKIVALLVLGSLFLGTGLSVAASAGEPDLDGVVYWSEDEVNSL
                     FSPDLMRTLNLTPGQRNSFQELRSRDWGYRQDHRYSRDLDALFSLGRMIRPGGSRVVI
                     ERGGSNNLDAFLAVLAFGILISQAQDNDRYYDPGYGYDYGYGSGQWIRIDDFLYLFFR
                     ILDMNQRRTFTVYFDRWYDDYYFRRDHRRDYERNYWEMMRDWDRRLRLSEKQRRDMER
                     YFRDLHNRQWERDRRYRDFEKDYLKRNWDRDSFRDIDKYRRDLLKRRQESLTPPPDSR
                     DRFRSILDDKQRRTFDSMISEREPRRWDRERTSPPKLQEIKSPPKVIQNKPAPPKFQD
                     KPAPPKIQNNPGPPKVQNKPAPPKFQNNPGPPKIQ"
     gene            complement(7914..8741)
                     /gene="proC"
                     /locus_tag="P5541_01610"
     CDS             complement(7914..8741)
                     /gene="proC"
                     /locus_tag="P5541_01610"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRV70700.1"
                     /translation="MEERSKTRICVIGAGIIGGAIAEALTPQYEVTATRRNCDKINGL
                     AGKGIKVTSDNRAAAEEADLVIISVKPVQVVPVLKEIAEAVRGKTVVSFAAAISTDIL
                     HMAAPEAHFVRAMTNIAVRVRKGYTLFSLQQDLSKERFPDLEKVLLNLGEIQQVEEQY
                     LDVLTAMSGSGPAYIFTVVESMIYGALREGLPRNLALKAAAYTAIGASCLLLETGKHP
                     SELRDMVLTPGGVTIDGIYELEESRIRTAFMKAIGAASEKGRTLSEEARAHALSDME"
     gene            9060..9269
                     /locus_tag="P5541_01615"
     CDS             9060..9269
                     /locus_tag="P5541_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRV70701.1"
                     /translation="MARWLIVAGGFLILLGLVLHFAPWLLTWVGRLPGDIRFETDRTR
                     VFVPITSMIIISVILTLVVNLLSRR"
     gene            complement(9307..9768)
                     /gene="alaE"
                     /locus_tag="P5541_01620"
     CDS             complement(9307..9768)
                     /gene="alaE"
                     /locus_tag="P5541_01620"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HRV70702.1"
                     /translation="MPKAAKIPEEGRNRIIMAVADIAAMIIFSTALCMMIEVFIAKLT
                     VSQSLQARMAAIPVNLVTGRPYGWFRDKLFAFLEIDQSRPLKMIFGDTLAFVIFQVPL
                     YVIVLLFAGATWRQIAVSSVFMTLIFSLAGRPYGIFLDWCRSLAFSINKRC"
     gene            10005..10961
                     /locus_tag="P5541_01625"
     CDS             10005..10961
                     /locus_tag="P5541_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70703.1"
                     /translation="MAKRLLFPLTVLLLLLVSANAVQAQDISGTWDVKVHGFNYFTDR
                     EPSRMAISETSTTMEIEQEGERVSITLGAFGGVHAATIFKGVVGNDRFVASWWYEGYP
                     YETKVLTGQYSPDSGRIKGKMIYPRAADKPGLVPGWLEVEFLANRKITLIPKPLEPVL
                     AGAIPLLLPEDCLSLSPSNVQVKNIGGRWKIVDGDHWILDFGNKKPEADKALNIIWSY
                     GLDKACYVGRPDPSMTYWTAGGQAPSGSLSGEDCIGFNPGTIEVKNVDGRWKIVDGSH
                     WVLDFASSREEAETALRIIKHYGFRYICFVGRPDPSMTYFRK"
     gene            complement(11036..12238)
                     /locus_tag="P5541_01630"
     CDS             complement(11036..12238)
                     /locus_tag="P5541_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV70704.1"
                     /translation="MLYRKVPKSGDELSILGFGCMRLPVKDGRIDEERATAQIHYAID
                     RGVNYIDTAWTYHGGESETFLGKALKDGYRERVKLATKLPSYLMRSREDMDRFLDAQL
                     EKLQTDHIDYYLMHTLTGPLWNCVDTMGARDFLDKARSDGRIVNAGFSFHGLPADFAP
                     IVDSYGWEFCLIQYNYLDEENQAGTKGLKYAASKGLGVFVMEPLRGGNLGLPEAPPPV
                     AAIWEEAPVKRTPVEWAFRWVWDHPEVVVVLSGMNEESHIEENLKIASEALPDSLTVE
                     EKSLVKRAADKYRDIMKVNCSGCGYCMPCPAGVNIPSCFEVYNKMHLFGNSEEGKNSY
                     VVKMSGITRRGESNYASQCVECGKCLKKCPQGLDIPLLLKDVVAELEGPDLEERALEM
                     ARRMNINS"
     gene            complement(12293..13060)
                     /locus_tag="P5541_01635"
     CDS             complement(12293..13060)
                     /locus_tag="P5541_01635"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRV70705.1"
                     /translation="MKYIKYQKDGQTGWGILEGNVISALTGAPWNTRELSGEYPLLEE
                     VCLLPPTEPANIFCVGLNYEDHAKESGSELPEEPHIFLKGTNSLLEHEGTILIPGWAG
                     RVDYEGELAVVIGKRCRNVSEAEAPGVIFGLTCLNDVTARDLQKKDAHLTRSKSFDTF
                     CPMGPWIVTGSETKGRKIETRLNDKVVQSSNTDMMGFPVPRIISYITRFSTLMPGDVI
                     ATGTPAGIGPMKAGDVVEVEIEGLGTLKNHCEADPRG"
     gene            complement(13097..14005)
                     /locus_tag="P5541_01640"
     CDS             complement(13097..14005)
                     /locus_tag="P5541_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV70706.1"
                     /translation="MKNDRVRGPGTGSLGQGTLFDNDHTAEESPERSISGVKVRDVNC
                     RSALNRTKIPGFDYCMNPYGGCTHGCVYCYASFMCRFSGHEEKWGEYLDVKANFPSIL
                     ARQLERSRTTPSGRVLLGTVTDAYQPAESRYRLTRSSLEILAGHPRMEVHVLTKSALV
                     RRDIPVLRKLKGCEVGFTITTLDEGIARIMEPGASSPLLRIETAEELMKEGIPVWVFV
                     APLLPGLTDRKDSVLELLAALGEKGIRHVGFDPLNPYPAVIERLRGTYRRHFPDQLVI
                     LEEFIKQPRNLRRETAKHFRRMTAGR"
     gene            complement(14037..14897)
                     /locus_tag="P5541_01645"
     CDS             complement(14037..14897)
                     /locus_tag="P5541_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRV70707.1"
                     /translation="MTLLSLSLLVIAAFLHAGWNLVAKRVAGGAAFTWLFSSFSVLLL
                     FPFALSATSGKGCFLGTELLLFASGSAVFETGYFLFLQRGYKAGDMSVIYPLARGTGP
                     LVATFLAIVVLGERPTPLNLAGTGLVVAGILWIAGFSPHLKDFRSRTAPLLGIATGVC
                     IAGYSLVDRMAVGERGFPPTLFFFAVIVIQVMFLTPLALRESEGILLQWTYYRRPAFT
                     IAFLATSAYLAVLYALSITPLSLIAPAREASIVIGTFLGATVLGEGYKKRRMTGSAII
                     LAGIALLAAG"
     gene            complement(15299..16234)
                     /locus_tag="P5541_01650"
     CDS             complement(15299..16234)
                     /locus_tag="P5541_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRV70708.1"
                     /translation="MRKVPALREGDTVAFISPSSQSSMDSIEKAAKITEDMGFKVKIT
                     RSCYESEGYHSGPDFVRADDLNRMFMDPDVSGIFCVRGGDGAIRLPQRVDLDAVYRNP
                     KVFLGYSDITILHLMLNRPRGFITFHGPMPASEFVRDSFPGYVRDSLLRAVTSTEPLG
                     EIVPYESAPPMEPLVPGTAEGETIGGNLALVCSLLGTPWEIDTRGKILLLEDTNEPVY
                     RIDRMLTQLRLAGKLDAAAGIVLGQFTNIQPRKPKDSFTLQEVLEAVVAPVGRPTLIN
                     GCFGHDVKKATIPLGVRAVLDGSRSTLTVIEAGVE"
     gene            complement(16259..17416)
                     /gene="truD"
                     /locus_tag="P5541_01655"
     CDS             complement(16259..17416)
                     /gene="truD"
                     /locus_tag="P5541_01655"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRV70709.1"
                     /translation="MKIKVNPEDFQVEEILARPLVQTPAAHRVYRLRKRNWNTLDALA
                     EIARSRDVELRDLHWCGRKDRSALTEQYFSTLSQVDMTFRSMNLSAEFAGFSDSRLGP
                     GSISGNRFRIVLRDLDAQESEGIDKRLEETKLFGFANYFDDQRFGDVSDGGVLAGEMI
                     VKRRYGEALKCIFTTFHPKAPERVKARKAEIASKWGNWKVLSPLCPEPRLRGMLEVLT
                     RGGSELQALGKLPREEMSQYLSSYQSFLWNLLLEGILKETVGNLFTIRCRPVDLLQYR
                     DLTREAFRRLAELVIPTPAAGMPEIPGGFGKVLSRVLYSRGVRLSKLRLPEIHQAFLS
                     SFGRRAISVPEEFKWEEAPDRLFPGRRIISLEFILPAGSYATMLIKSVNRG"
     gene            17526..18053
                     /gene="cobO"
                     /locus_tag="P5541_01660"
     CDS             17526..18053
                     /gene="cobO"
                     /locus_tag="P5541_01660"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV70710.1"
                     /translation="MLFDPGLVQVYTGNGKGKTTAALGLSLRVCGHGGKVAFIQFMKG
                     WDFYGEIAGLKLLQGVRHERTGTADYVNRGSESEVDRSEAAKGVSLALECLSSGSYDL
                     VVLDEINVAVDYGMVDPEEVAQAIIGRPRHVEVVLTGRAAPKAFLDLADLVTEMREVR
                     HPYSKGIRSRRGADF"
     gene            18221..19519
                     /gene="brnQ"
                     /locus_tag="P5541_01665"
     CDS             18221..19519
                     /gene="brnQ"
                     /locus_tag="P5541_01665"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HRV70711.1"
                     /translation="MSKEKQDLLSFGALAVISAALFSGHFGVGDVIFPPILGKGSGAS
                     WFTAAMGYGIINSLGVLVAYLAVARQQKTLLEMSSRTLGRAFGVVFTTICMLIIGPVF
                     ILPRVSSATHEMAVALFFPNFPLVGTLAVFFALNYWVAFNRAQVIDRLGKILSPALII
                     FMAVLIIKGIVSPIGPVPATGSGNPLADGVLNGYNTMNALGAALFGGWILKELHLRGI
                     TDKDAQTRNLSYIGPAVALGLLVTSTGITYLGATASTIFPDAGIGVYTVMIAEGVLGA
                     VGKAVFALLLAFACFTTSVGLTSTAGDVFQEMSGGKLQYKTIVAVSSLVGFGIGTVGL
                     GKIVGYTVPWLMLVYPAIIVLLVGSLFDFSKYKPALQGAIITAVILSIGDFLGGMGMA
                     DNPFSAMTAKLPLGGQGLGWLVPALVVGALAMLLPGRSKK"
     gene            19541..20419
                     /locus_tag="P5541_01670"
     CDS             19541..20419
                     /locus_tag="P5541_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV70712.1"
                     /translation="MQFRGELDSARIVTVLDDYAGYETSFLAQHGIAILLEASSGGVC
                     KRVLLDAGQSALPILHNLEIMDIDPGSIDAIVVSHCHYDHTEGLVEVLRAVGREGIPV
                     IAHPTMFRPNYIFKPFIRNIGVTGKNGKVAIEEAGGLLVLVDEPFEIMPGVIVTGEVP
                     RRRAFEKQGIGTYNIEGGKVVPDEIRDDLSLTVKVKGKGLAVVSGCSHAGIVNIIDWC
                     REIAAEPRVDLVIGGFHLIEASGERIRETAQALKQLNVGRIVTGHCTGLEALCEFSRI
                     LGDRFEQLHVGTTIQL"
     gene            20489..20905
                     /locus_tag="P5541_01675"
     CDS             20489..20905
                     /locus_tag="P5541_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70713.1"
                     /translation="MIKGCLAVFLSASLFFMTVSAGFACSEYDPLVGGLEREGVIEAL
                     QGELLESFGIEAEFVFNWFGIEDVWAWVESEPRSPDGLNRYEPFLALLKKEGENWAVT
                     EVPPLEDGSPPVDDAYFRSLMERYPGLPEGIFPWER"
     gene            complement(20911..22077)
                     /locus_tag="P5541_01680"
     CDS             complement(20911..22077)
                     /locus_tag="P5541_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV70714.1"
                     /translation="MKKRRLKILATSGIIILLAVTLFFLLRPEKMAAVRVVRGEISSG
                     VEEDGYLQPLEDRNFYATQTARITELPVEAGDEVSQGQLLIGMTNPDLEAILAETRAY
                     WRQAEKSEIGYAAAAASLRLLLADENRNMERYRKLYEAGALSMTEMEQVSLRVERYTK
                     DLAEAEARLGSASALSRGLRQQVLELEKKQAELTLKSPIDGYVLDLPVKKEQVVMAGE
                     LLVALGIEDEMEIRSDVLSDSLGGVKVGSRVRITAPILGSLTLEGHVKKIYPLAEEKV
                     SPLGVAQRRVPVIIAFPMSEVLKPGYEVRVFIETSRREDVLLLPVESVRTEDGIRKVF
                     QLQGGKIHATEVRIGITDRRMVEILEGLEEGDVVVRDASLDLSDGQRARAAEDI"
     gene            complement(22091..24469)
                     /locus_tag="P5541_01685"
     CDS             complement(22091..24469)
                     /locus_tag="P5541_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70715.1"
                     /translation="MPVLARKLLRTIGSTLGQFIASAVVIATGVMAFVTMTAMSDNLI
                     RSRDSFYRETDFADHFFHVVRAPEGVLSRVEAVPGVLRASVRIQKDVPVINNEDERTT
                     IRLLGYPLPDRQELNRVKVLSGRMAEKYPEGGAVEILVNRQFAEAHGFRQGDTIDIAA
                     EGRRKTLTITGTAAGPDFVYAVKDAASMLEDPSSFGVAVLPQNQLQQILGEKGSVNQV
                     LVRFIPGADRVGVAREIRNILEPYGSLADYEQKDQISEAILRGELNQLKAFARFLPTI
                     FLGIAVLMQFVFIGRMVQVQRGQIGIMKAVGYGNVSLLALYGGYALSAASAGAVAGTG
                     LGYFFAGLMTKLYMMFFNLPRLEEALHFRAAGLVFGTTLVVGTLAGVWAARGVLAVSP
                     AEAMRPATPRSFRPSMIENIPGLMEHLGLSWRMSLRSMFRNRFRSAVTILGLLLAVGM
                     IVISIFARDSMFDLMERHFEKEWRFDFLVRVVNPVKEKDLLSISNLPGVREAEPILEL
                     PVRIHYKGRYREDVLFAMHTGSGLGRVIARDGHELDFPEEGLFLDWYTGRILGAGTGD
                     EVEVETILGLGPPRKSMLRVAGISRKAFGSASSVSLDTANRLLNEQGIASGIVFNTQD
                     EFKESIEYALGDMINVISVTSREKEVRFLEKNMKYLYYSVGIMTFFSVLLGFAIVFNA
                     SSLSFSERRRELASLRVLGFSAREVSSLLWKENLLLLAVGAVLGLPFGRLMAEAYAIG
                     VSTDLFTFEAVVYPTTYAISVLGGGLFILVSWGLAARSVGRLQPVETLKAND"
     gene            complement(24469..25182)
                     /locus_tag="P5541_01690"
     CDS             complement(24469..25182)
                     /locus_tag="P5541_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70716.1"
                     /translation="MQEKGPTWRLDRVFKTYDMGEVKVEALRESSLSIPEGELVVILG
                     PSGSGKSTLLNLLGGMDVPSGGDVWFRDENLSAASDRQLTLYRRNVVGFVFQFFNLIP
                     DLTARENVDIAAELVDSPFPTEDVLDGVGMSDRIDHFPSQMSGGEQQRVAIARALVKN
                     PLLLLCDEPTGSLDASTGRLILAQLEEINRKFGTTVVIVTHNASIADMARRVVRMSSG
                     RIVEETYNEEPLPPERISW"
     gene            25300..26796
                     /gene="malQ"
                     /locus_tag="P5541_01695"
     CDS             25300..26796
                     /gene="malQ"
                     /locus_tag="P5541_01695"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRV70717.1"
                     /translation="MGRSCGVLLHISSLPSRWGIGDLGRGADLFLDFLCSSRQSLWQI
                     LPLSPTDPGQGNSPYSSFSAFGGNPLFISPELLVKDGLLELSDLDSHEPLSMKACDHA
                     GSARIKRSLLQRAYKRFASSEDSEYYLFCEKNGHWLDDLALFLSLKRHLGGVPWCEWP
                     APLRDREEKALAEAREQLADKIGYARFLQYVFERQWGRLKRVCREKGISVIGDVPVYV
                     SHDSADVWAHRELFDLDPTGRAKKVAGVPPDYFSESGQRWGNPVYRWDMLEKEGFWWW
                     IRRLSNALSLFDMVRIDHFRGLVKFWSIPSSERSAIDGEWVEASPDAFFLRVKENFPA
                     MPFIAEDLGYITPDVKDYIRRLGIPKTLVLQFAFGDDLEKSPYAPANHIENACVYTGT
                     HDNNTTRGWFESEATPLMRSQLNEIAGHKVTPETVSMDMISIAMASKASMTVYPMQDV
                     LGLGQEARLNTPGKPSGNWLWRLEERQLEDAPVEKLAEMTARFGREAR"
     gene            26865..28601
                     /gene="glgP"
                     /locus_tag="P5541_01700"
     CDS             26865..28601
                     /gene="glgP"
                     /locus_tag="P5541_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRV70718.1"
                     /translation="MPKMNYGNDIGHSLRFMMEQDPAFRTVAYFSMEVGLKASIPTYS
                     GGLGVLAGDILKSAADLGVPIVGVTLLYRKGYFRQSFEDCLQKALPVEWDPEKQLALL
                     PHEVTVMIEDRVVKVRAWCLELQGRTGFTVPIYFLDTDVGGNSPADRELTWYLYGGDE
                     RYRLCQEIILGSGGLRMLRDLGYSNIDDYHLNEGHAAFLALELIREMGYENYDRVREK
                     GIFTTHTPVSAGHDHFSWDLINRVMDRSMAARLRRMMPTEDVSMTEIALRYSRFINGV
                     SEKHTKVSRAMYGREDVDCITNGIHTLTWVSPDLAEVFTKYIPGWDNAPERLVKAVQI
                     PVEEIRSAHAPAKKRLLDYVEEKTGKKLDPERLTIGFARRVAQYKRADLVLRDTSRLV
                     KAAGGKVQFIFSGKAHPNDNPAREILRKLLCEAQNMVGIDIPVVFIEDYDMDKAALLV
                     QGVDLWLNNPVRPREASGTSGMKCALNGIPNFSVLDGWWVEGCVEGVTGWSIGPEPKE
                     SISDQYDDSVDLDDLLEKLEKVIIPTFYERPREWGEIMRGAIALNASYFNTHRVVREY
                     CEKAYGIQMRGL"
     assembly_gap    28626..28725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28726..28907)
                     /locus_tag="P5541_01705"
     CDS             complement(<28726..28907)
                     /locus_tag="P5541_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HRV70719.1"
                     /translation="MPIVRVDLWSGRSQEFKAELAKAITDVVVQKVGCPVEAVTVKIE
                     ESPVENWMIGGKQCEK"
     gene            29115..30392
                     /locus_tag="P5541_01710"
     CDS             29115..30392
                     /locus_tag="P5541_01710"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV70720.1"
                     /translation="MLNRDSQDLASEWILLNQEVMSLHEKGQYERAASVALRTLELAD
                     RFGDPDHPYLAASLNNLAGIYDAQGRYLEAEPLYQRSLEIRERVKGSGHADVAATLNN
                     LAELYRAQGRYFEAEPLYRRSIRILENVMGPDHPDVATCMNNLAGLCDARGNYEEAER
                     LFRKAFEIRSRSLGKGHPDVAVTLNNIAALYENQGWFTEARPLYRRALSILRKALGSD
                     HPDVATCINNLAGFYRTQGKYAKAEKLYKRSHDIRKKSLGANHPYVAASLTNIAELYF
                     AQGKHSQAESLFKRSIAILERALGPDHPDVATGINNLAGLYDTLGKHEMALPLCRRAL
                     AIRTRSLGRWHPHVAVSLNNLAGLYESRGMLQQADPLYGRSLQILEKTLGPAHPDVAV
                     CLDNMADLMRRMGRDEEASRMEQNARKIRGEEE"
     gene            30525..30914
                     /locus_tag="P5541_01715"
     CDS             30525..30914
                     /locus_tag="P5541_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70721.1"
                     /translation="MDQRSSEMKVVYELEYDEEKKIITGRITGELNRAVAAAMASRLA
                     ELSLRHGCFRLLNDLREADLIPSVLDIYMIPREVEKAGILKTFKRALLVRPPLDDFRF
                     LETVSFNEGHRLRVFRDPEEALQWLLE"
     gene            31017..31232
                     /locus_tag="P5541_01720"
     CDS             31017..31232
                     /locus_tag="P5541_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRV70722.1"
                     /translation="MSGVYKVIELVGCSETSWEDAACCAVETASKSLRDLRVAEVVMQ
                     DVTIKDNKIDQYRVKLKVSFKYHCEED"
     gene            complement(31297..31764)
                     /locus_tag="P5541_01725"
     CDS             complement(31297..31764)
                     /locus_tag="P5541_01725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRV70723.1"
                     /translation="MLSVRETVIGPVGIEEDEGSITRLFLPNKARRIKGPDGEGTPLL
                     AEAFGQLDLYLGGKLQRFSLPLRPEGTEFMKRVWRELLKVPYGRTTTYRELAIAAGSP
                     GGSRAAGMANAKNPIAIFIPCHRVIGSNGKLVGFGGGLELKSWLLDLEARNME"
     gene            complement(31813..32643)
                     /locus_tag="P5541_01730"
     CDS             complement(31813..32643)
                     /locus_tag="P5541_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV70724.1"
                     /translation="MEKDIIRDPLCIDETYPPDMHSLFIQERLLGLIYLADGEGPHKT
                     LLLLHGFPGNEKNLDIAHMIRRGGYNVLVFHYSGSWGSRGSYSFSQNYSDLQAVRSIM
                     ADAAFAREHRIDRDNIYLAGHSVGGFLTLLAARDGLDFKGFAPLAPYDLGKQARRIRN
                     EDKEAFRETLELFRGGMAPLSGATPESLMEEILDNHDRWDLLGAPSAYSERRMLLVMA
                     AEDRCAWPKIHQIPVAETLARAGGDKTVISLSCSHDFPDKRVSVAEAIFEWLEEKEER
                     "
     gene            complement(32684..33634)
                     /locus_tag="P5541_01735"
     CDS             complement(32684..33634)
                     /locus_tag="P5541_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HRV70725.1"
                     /translation="MTKVLDNVLEAIGNTKIVNCSRLVEHWGVSGTILAKLEHLNPGF
                     SKKDRVGLHMILEAEEKGWISPGDTVVELTSGNTGTGLALACGIKGYRFVAVMSEGNS
                     MERALMMRALGAEVVLVPQAPGSIPGQVSGEDLELVDQEARRIVERRGAFRADQFQMR
                     GNMIGERLSGEEIWRDTEGRVDAFVDMAGSGGSFTGISSVLKEHDPRIRCYLGEPAEA
                     AFFGNPGGSGRHRIQGCGYSRELPLLDRSLVDGFVSMNDNEAIEGARALARFEGIFGG
                     FSSGAHAAAARRLLEGPEKGKTVVFLVCDSGLKYLSTDLY"
     gene            complement(33740..34432)
                     /locus_tag="P5541_01740"
     CDS             complement(33740..34432)
                     /locus_tag="P5541_01740"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRV70726.1"
                     /translation="MKKLGLVGGIGPESTLDYYKGIIDGYRKRTGGDDYPVMVIDSLN
                     LTEMYNLASKKDWKPFTDILVESTTRLVAAGAELAAMAANTAHIVFDEVKKRSPLPLV
                     SIIEETCHYALSKKCKRVVIFGTAFTMSSGLYTEAFARYGMEAFVPTGKDQKTIHNII
                     FPHLQEGIVIPEEKKAVLEIARRMISDKRADALVLGCTELPLLIREGDLELPLLDTTQ
                     IHIQKIVELISA"
     gene            34606..34941
                     /locus_tag="P5541_01745"
     CDS             34606..34941
                     /locus_tag="P5541_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRV70727.1"
                     /translation="MITFEEFQKVDMRVGKVVEIEDFPRAKNPSYRVRIDFGEEIGIK
                     SSSLQAKKDYTMEEMLGRYVVCVVNFPPRNIAGFMSEVLVLGVPGQDESLVLLEPERA
                     PVPLGARVF"
     gene            34972..35367
                     /locus_tag="P5541_01750"
     CDS             34972..35367
                     /locus_tag="P5541_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70728.1"
                     /translation="MTQLIVRWAARVTGVGCLLLFLVFFIGEGDIRDIVYLQPREVVL
                     ILFVPVLFSAGVLVAWWREPQGGILILASVLWFNVTDIVSERKFTGEIEFAFLIIPGL
                     LFLLSWYLDGRRRETHGPGIPPGNGKPRS"
     gene            complement(35516..36694)
                     /locus_tag="P5541_01755"
     CDS             complement(35516..36694)
                     /locus_tag="P5541_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV70729.1"
                     /translation="MTNIKERAKELNGKIIEWRRHIHRNPETGFETPMTEAFIVERLR
                     EMGIEEILEGVGKHGVTALIKGERPGKVLGMRADIDALPVKEETGLSFASVNGRMHAC
                     GHDAHVAILLGTAKMLAENRKDLPGSVKLIFQPSEENSEGAPAMISDGVLENPSMDAI
                     IGLHTGNLWEGPSTGMVGYRYGVLMAATDWFTVTFEGKGGHGATPHLTVDPVAMACQA
                     YIAIQTIVSRETSPLNSAVITVGSINGGSAPNVIGPSCTISGTIRSLDAGTRAMLSER
                     LKTVCKGIAQSMRGRAEVQLITGAPPLNNDSEITDKLREAAASIVGEEAVTEIAEPTM
                     GGEDMAFFLEKVPGCFFFLPSMPGEGEVFPHHHPKFDLDESVLWIGSAVMAHFAMTWQ
                     "
     gene            complement(36987..38066)
                     /gene="rsgA"
                     /locus_tag="P5541_01760"
     CDS             complement(36987..38066)
                     /gene="rsgA"
                     /locus_tag="P5541_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRV70730.1"
                     /translation="MDLGNIGLSQELMAGSRLYRGLHIGRIVSQQRDLYRVVTEDGEV
                     RAAVSGRFLFKTGGPCEFPAVGDFVMLDRCDSTSGNAVIHKVLPRKSAFIRKAAGRNR
                     DSQVVAANIDTVFICMSLDNDFNLRRAERYLGIAWESGAIPVMVLTKEDLCDNLTVKL
                     SKLASVAPGVEVIVTSSLTDDGYAPLYQYLGRGRTIAFIGSSGVGKSTLINRLLGSEI
                     IRTGDTRNDGKGRHTTTRRELFVIPNGGVVIDTPGMRELGIERADLSKAFSDIDELAS
                     RCRFHDCTHTSEPGCAVLQAVREGLLPEDRLANFMKLEKESRYEDLNSRQIETEKITA
                     MFAGMGGMKNARKFAREKNEKRHGY"
     gene            38758..39918
                     /locus_tag="P5541_01765"
     CDS             38758..39918
                     /locus_tag="P5541_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV70731.1"
                     /translation="MLDEKEVVGFLSELIKVDSVNPPGNEKNAALLAQRKLEEYGIPS
                     EIMDVDSEEPNRANLYARMGDPSQGPALLYSGHFDVVPAGEGWVHDPFGAEIEGDLMY
                     GRGTSDMKAGDAAMIMAMCILKKADVPLSGTLAFLGTAGEETGLIGARDFAKKHGSER
                     IDALAISEASVGDIYIAQKGAFWLKFVSKGKRAHGGLPEKGVNALKNMIRFTERLQEE
                     FKFKVTKSPLLTPPTLNLTGMHAGELTNIIPDLCEAVVDIRTIPGNPHSEILAQIEGI
                     LSELKKEDETIDISIQVELDLTSISTDVDHPFVKKAVESYVGVFNKKPEIKGVTYGTD
                     AVPFREIDPDLPLIIYGPGDSSRNHKLDEYVEVSSVIDSTKFYIALALEYLK"
     gene            40031..40576
                     /locus_tag="P5541_01770"
     CDS             40031..40576
                     /locus_tag="P5541_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV70732.1"
                     /translation="MRLLVAFVNWVNRLNDWVGRIVGFLVYPIMFVLVFEVVMRYAFN
                     RPTIWAHETSCMLYGAHFLLGGAYALSKDAFVNVEVFYQSFRPRVRAIVDLLTWTMFY
                     AFVGTMLWKSIPWALASLRVGEYSESVWGPPLWPIKLTLPVAAGLMLLQGMTKTAKDL
                     YLALTGRELVPAVEEKAVAEN"
     gene            40586..41899
                     /locus_tag="P5541_01775"
     CDS             40586..41899
                     /locus_tag="P5541_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV70733.1"
                     /translation="MDIGIISILLFGSMFFLLAMGLPVAFVLGGLATVFTAVFWGPES
                     LFIIVARTFAMMQTTTLVAAPLFVLMATVLERSGVAEDLFEVMYRWIGSIKGGLAVGT
                     VLACTLIAAMSGIASTGVVMMGVMALPAMLKRGYDKRMATGCILAGGVLGPLIPPSIG
                     LVLYGTIAKVSIGELFAGGMGAGLLCSSLIILYILLRCHFQPDMGPAIPYEERSDWGA
                     KFASLKGVILPLLLITSVLGSIYSGMATPTEAAAVGALGAFVCSALHRRLDWKLIADV
                     ARTTIRVQGFIMWILFSAQAFAAVYMGLGAATLVTRLVETYEIGWWTMLISIQVVWFL
                     LGCVIDAWSILMITLPILMPLIPVYGFDPVWLGVLYAVNTQTGYLTPPFGAMLFMIKG
                     IAPKSITMKDIYNSITPFVITQLVCLALCIVFPSVVTWLPRLLFK"
     gene            42035..43096
                     /locus_tag="P5541_01780"
     CDS             42035..43096
                     /locus_tag="P5541_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV70734.1"
                     /translation="MKVRNLFLTGIALALLGFVTVGSSSPATAADEVFHWKWQAHQTA
                     GSLPCETIIPRFIKRVDEMSGGRLKISLHYAGELVEFMEVLPALQANMIQIANTGALF
                     WRGSVPMGWLTAGNLPPFITRSDAEFNELYHYRGLDALLKEAWGEHGVHFLGSHNVGD
                     TYFWSKKPINSLDELKGFKVRFFGAMSDTMQHFGASPVMLPHPEVYMAISTGTLDGAG
                     TCWWQYRDLKHYEVCPYFIGPPWQVPQAMELLVSQKAWEALPADLQAIVETAAIVLSK
                     EFADLTRLEEKEMFQKSFEEWGTTYIEWGEEDVDRITKEFSLPYLDKIAAEEGSKDPR
                     VVKGVEIIKQFMQDYGYID"
     gene            complement(43354..44538)
                     /locus_tag="P5541_01785"
     CDS             complement(43354..44538)
                     /locus_tag="P5541_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRV70735.1"
                     /translation="MEHGNRTEEPLIKSTTLTDRRLPELDAARGLIMMLMALDHTMEL
                     VCRKGFFEFWAMCVPDYGSLQAFFTRFITHTCAPGFALIMGAGMVFFATSRRSVGWEE
                     SRIIRHYWIRALVLVVIMLTLENFVWQQAFPSRPDSPVVSLEFGVLFSLAGSMALGSF
                     LLKAPDGALAGIGLTLALTTQWIITSRLGSPEPMSGVSLPLMFSFVPSGIRTPTLGIF
                     VLYPVLPWFVFTLFGMILARRIQADRPGTGKVLPLAGSALIAAFVLLRTTGGFGNTHP
                     VKGSGIMAFLALNKYPPSLAYMLLFSGVNLLIISSFSRAGHFWARKDNPLQVFGKCPF
                     FFYILHLFFFLAVGKLFPAPPGYWGGYACWATGLSALYWPCRAFVKLKARSSPDSIIR
                     LF"
     gene            complement(44772..45626)
                     /locus_tag="P5541_01790"
     CDS             complement(44772..45626)
                     /locus_tag="P5541_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRV70736.1"
                     /translation="MDLDKILVNDNFVSSGKSKDIYLIPDGKYKGKYAFVFTDRGTGY
                     IDKDGKPVFDPGYDNVVGEIPGKGAIACKFATYFFRLLARQGIPTHYIDTVADNVMIV
                     EPATPISMSEQGPEFPGSAPLLNLEWTWRNSAMGSFWRRYPFIRPCADLPQVVEAWTK
                     GKIDRLITFGSLTGAGVMTEEEVSHVKDFVRKIALVIYDEFAAHGLHVLDGKIELGRL
                     KDGDGRIVLIDEISPDVLRVCKGYQSGEKGCTRAPECISTRISGEDRKISAKYQLPAS
                     ELEKIFLK"
     gene            complement(45725..46696)
                     /locus_tag="P5541_01795"
     CDS             complement(45725..46696)
                     /locus_tag="P5541_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV70737.1"
                     /translation="MLTFGPVPSRRLGRSIGINSIPPKTCSYSCVYCQIGRTTVFTSN
                     RSSFFKPREIGKALEERMEKVQSCGESIDYVTFVPDGEPTLDENLSEARRIVKQFGFP
                     TAIITNASLMGKDEVREDLAGFDLVSLKVDSAIPEVWLHIDRPHPSLQLDAILEGSLV
                     FARNYKGKLLTETMLVRNLNDTEESLRTTAEHIGKLKPHCAYLSIPTRPPMEKWVEHP
                     TARSLLRAFSLFSVNIERTELLTGYEGNAFAYTGDPEADILDITAVHPMRRDAVEDLI
                     TRSGAEWGLINDLLRRGKIRKASWEGQDFYIRTPVNGKEEDPDREDF"
     gene            complement(46899..47870)
                     /gene="speB"
                     /locus_tag="P5541_01800"
     CDS             complement(46899..47870)
                     /gene="speB"
                     /locus_tag="P5541_01800"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRV70738.1"
                     /translation="MKPGNDFYCRQGGDNPMHAPRYCGIPTFMRAPFIEDPVEIDIAL
                     VGVPYDGAVEARSGTRQGPRQIRDMSSMMRSIHHVTRVNPYELCRVGDVGDVPFSRIF
                     DVEASHRDITSFYERLNNAGVWPLSAGGDHSITLPILRAIAMDRPLGLIHIDAHTDCC
                     DEEMNSKYSHGTPFRRACEENLIDPERMIQIGIRGAANSPECWEFGERNGIRIVFMEE
                     FARIGVERTVEEARRIVGNGPVYITFDVDGLDPAFTPGTGTPEIGGLTSLEAQGLIRG
                     FRGLNLVGADVVEVAPPFDPSGSTALVAATVMYEILCILAGSRITNS"
     gene            48067..48396
                     /locus_tag="P5541_01805"
     CDS             48067..48396
                     /locus_tag="P5541_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70739.1"
                     /translation="MRLKVLLITAAAILLMVLSVAPPSDAAFYNGQDIDGIVFDGAIY
                     LFDFDSGEPVKAVFSGYQVTIYWQNGEISKYLIDPLEIEIPEYIELFTDPEEPQPLVD
                     PILEIFL"
     gene            48448..>48750
                     /locus_tag="P5541_01810"
     CDS             48448..>48750
                     /locus_tag="P5541_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRV70740.1"
                     /translation="MKKINIGNNPYPLPMPVVIVGSSIEGRPNFMAVAWVTRVNARPA
                     LMSVAIGRHATASAIRDTGEFSINIPSVELVKETDLTGMVSGNDLDKSVLFDIFHGS"
BASE COUNT        10651 a        13157 c        13330 g        11412 t
ORIGIN      
        1 cgtcggcgcg acagctgacg aagccgtgat ccaccaggca cctcctcaca aggacataat
       61 cctcggcaat ctttgcaagg agggtgttca gttccttttt cgagtatctg actccctgtc
      121 caaagggctc cacgatcttt ctgggaagtg cgacccgctc cctggctttt agggtaaaag
      181 gcaacagggt accgtcttcc cggaagtact tccgtgttat cttttcgtat tttcccctgg
      241 taagggcata ctgttcatcg gccatcctcg agttggcgga aagatccaag agccttgcct
      301 ccggctttgc cttctgatcg aggatctgca tcattgccag aaagaccttt gcgtgcctgg
      361 cacgtccctt catgcggaaa cggtgattcc tgactgtgga gcggcttatc ccgagttccg
      421 aagcgatctt cttgtcgtta tcaccccgga agaacgcctc aagcagtttt gtctgggggg
      481 cggagacgcc cttctactgc ttcatgaggt tcagcaggta gttgaactga ggtccgtgtt
      541 cactttcgga atggatcctt gccgcggctt cggcctccaa ttagcggtct cccaactgga
      601 agccccccca ccgcaaaggt cttcacacac ttcaggcaaa ccttcgccat cgtcccagga
      661 tcctacaaag cgccttcagc cagctcttct atgtatgcta tttggaagat tcaaagggta
      721 gatttgttaa cgtccatctt ctttggttgt ttaataaaag gcatgtacag tcagaagata
      781 taatgcctga acatgttagc ggtaatgtca ttcccgccgg ctcgtgttgc aggatctttt
      841 ccagatcgtc ataacagggg ttccttatta ttataatttt gcctcagctc ctagaccggt
      901 ttatccggag agagtgcaag caatgcatgg gtcatggtat aagtttggca taggaccatc
      961 aaatgcatga aaggaacgga tcaaagatga cctggcgatc aaaaagactt ggacttttca
     1021 gcggcagggg gtcggcatcg cgccgcgcga tctacaaggg ttgcggatac gatgacgagg
     1081 acctctctcg cccgctgatc ggtgtggtga ataccgcaaa cgacgcaggg ttggggcacg
     1141 tgcatctcga caggctcgcg gcgcgtgtaa aggccgggat actccaggcc gggggcacac
     1201 cctttgaatt cagcaccata gccacctgcg gggcggtgcc cattgggact ccgcatcttc
     1261 gctacgaact ggtcataagg gacgttatag catcatcggt ggagataatg gctaacgtcc
     1321 agcttcttga cgggctggtc ctgctggcat catgcgacag cataattccc ggggtactca
     1381 taggtgctat ccgctcgggc ctcccttcaa tattcctttc cggggggccc cagctcgtga
     1441 cccgtttcga aggccgccag gccgtgatga gcgaactgga ccagcttgtt ttcggcgccc
     1501 agtacggcga tgaggatgtg tcagcaaagc tgcgttacct tgaggataac gtatgccctg
     1561 gcccgggtgc ctgtgccctc atggggacgg ccaacacaat gcagatcctg gcagagggca
     1621 tagggatgtc tcttccggga tcggcgacag ttccggccgt ttatgccgag aaggagcgat
     1681 ttgccaccag gacaggacgc aggatcgtcg atatggtcaa ggaggatctg accccccgga
     1741 agatcatctc cagggagaac ctcttaaacg gaataacact gactatgggt atagccggtt
     1801 ccacaaatgc cgttctgcac cttctcagcc tggcaaggga tctggatatc gatctggacc
     1861 tggacgattt cgaccggata tcccgggagg tccctgtgat atcaagggtc attccaacgg
     1921 ggaaggcaac cgttgtcgac cttcaccacg cggggggagt acccgcgata atgggtgaac
     1981 tgggagagta tctccatggc ggagccctca ctgtatccgg cctgagggcg gaggatattt
     2041 tcagtaaaga gagatcgaaa gacctttcgg ttctgaccac ggcagataag cctgtccttg
     2101 ctaacggcgg aatcgccgtc gtaagaggca accttgcccc cgacggagcc atctgccgga
     2161 cctcgactat atctgagaaa gctcttcgtt tcaaaggtcc agccagggtc tttgattccg
     2221 acgaggaagc ccattccgct gtggtggagg gaaagatctc caaaggggac gtggtggtta
     2281 tccggcgtga aggaccacgc ggggccccgg ggatgaggga gatgatgatg accaccgacg
     2341 cactcgtcgg tctggaaatg ggtcaggagg ttttcgtggt caccgatggg cgcttctccg
     2401 gttttaccga gggcgccgca gttgggcatc tttcaccaga agcagatgtg gggggaccca
     2461 tagccgtcgt ccgggaaggg gatcccatat ccatcgatat cccggcacga agagtggacc
     2521 tcgatattcc ggctgaagag ctgaaaaggc ggctttcgga gtggataccg ccgcaaccca
     2581 aggtgagccg gggaatactg ggtctctgcg caaagacagc acttcaggct cacctgggag
     2641 cgatgctgga ggacagggta agtaacccaa aggcgtaaac agtaaacagt aaacggctga
     2701 attcggcaaa acctttgttt tcctgcagat ctttccgttc accgttcacc atctttttta
     2761 gggaggtttt caagatgaag caagagatcc gaacagacag gtcgccagat ccgatcggtc
     2821 cctattccca gggccttaaa gtgggtaacc gcatctacgt gtcaggacag gggccgctga
     2881 accacgaaac cggcaaaacc ccggagacga tcgaggaaca gacccgtcag gtcctgacca
     2941 acatcaggaa catcctggag gccggcggtg cgaccatgga cgacgttgtg aaaaccaccg
     3001 ttcacctcgc cgacctgaag gatttccagg ccttcaacag tgtctacaag gagttctttt
     3061 ctcccccctt tccggtgagg accaccgtgg gaagccaact gctggggatc cttgtggaga
     3121 tagatgtgat cgctgagatc cagtgatcgg tgtccactgt aaaggaatgt taaggaccct
     3181 ttacaagaga aaacctttct tgacagcagt tgagggatca atgaggtagt ctgacaaaga
     3241 ccttcctgtc atggggggat tgccgtggag cgtatactgc tagccgatga tgataccggg
     3301 ctttgcgaat tgcttaccga gttccttgag tccgaaggat tcaaggtaga atccattcac
     3361 gatggacgca aggtggcatc cagggttctc gcagaacgtt tctcgctgat catccttgac
     3421 gtaatgctcc ccggtctttc gggaatggag atcctgaagg agatccgtcg tcaatccgct
     3481 gtgcctgtcc tgatgctgac agccaggggc gatgatgtgg accggatagt tggccttgaa
     3541 cttggagccg atgactacct ccccaaaccc tttaacccca gggaactggt ggcccggatc
     3601 agagccgtac tgaggcgggg ccggatggac agcagccgtt cttcccaaac aggcacctac
     3661 cacgtagggg acatgaccat agacccggcg gcgaggtacg tgagcgccgg cggtaaacgc
     3721 atcgagctta ccaccgtgga gtttgacctt cttgagaccc tggcccattc ggccggcact
     3781 gcaatctcga gagaggttct ggtcagaagt gtactgaaga ggtccttctc ccccttcgac
     3841 aggagccttg acgttcacat cagcaacctc cgacggaaga taggcacctt ctctgacggc
     3901 tctgaccgga tcaagaccgt cagggggctg ggctatatcc tcgccctgcc gaccgaggag
     3961 tctggatagt gagagagaga aagctgttcc agaggatatt cctgtggttt ttgggggcca
     4021 tgctcctgat ggtccttgtc agcgttcttc tgacggtctt catgacccag caggggataa
     4081 tacttacagg gcagcaggaa gccctttcga acgctctcga caggaatggt aaaggactgc
     4141 tcgaagtggt cgagacaggg aaccccgccc gcctaagaga gtccatagac aggatcagga
     4201 gcgaaactgg tcttggaata tttatcttcg acgtagatgg agtacctgta cccgtcgaag
     4261 gtggtccctc gaaagatttt ctggagaggc tgggcaggga tgccgcggat cttctcggtc
     4321 agggtaccac tttccagacc aggggtgagt tctcgtcggt cttcaggcaa cttggttctc
     4381 cttctggcaa ggactacatt cttgtggggg ttttccggag atcaccctac atcgcaaggg
     4441 tcctcaccgg gaaccccaag gccctgctga tccacctgat gggcctttta gccaccgctc
     4501 tagttttctg cttcctgctg gccaggtacc tttcggaccc cctggtgcgg ctgagcaaga
     4561 cagccaggtc cgtttcgagt ggccaactgg acacccctgt ggaggacctg gtcaaggacc
     4621 gatccgacga gataggcgag cttgcccgca gcttcgaggc catgacaaaa catcttacga
     4681 ccctgttgga agctcagcgg cgcctgatcc gggacatatc ccatgaactc cgcacccccc
     4741 tggctcgcct tggagttgca ctggagctcg ccaggaagag atcggggact gtagcaaggg
     4801 agtccctcga acggatagag cgagaatcag aggtcctgaa cgatatgatc ggcgacctgc
     4861 tctcactgtc aaaggtcgag gctgagatgg atcacgtgac cttcgagatg gtcgatcttg
     4921 cggctatcct ggaaaatgtt gtagaagacg ccaacttcga ggcgcgggtc attaagcgaa
     4981 gggtcgagct tgtctacccc gatgacctgg agccgataag actcaacagc gaactggtcc
     5041 gaagggccgt ggagaacata gtccgcaatg gcctcaggta cacccccgag ggcaccgctg
     5101 tttccgtaaa ggcagaacga cggagcaggg gggggcggcc cggtgtctct ttgacggttc
     5161 tggataaggg ccccggggta cctgaagacg aacttcaaca gatattcaaa cccttttaca
     5221 ggacagaaac atcccgatcc cgtgatacgg gaggttccgg cctcggtctt gccatagccc
     5281 agaaagctgc ccttgcccac ggtggagaga tcaatgccga gcttccgtcg ggaggcggtc
     5341 tcctggtgga aatgtggttg cctctccagt ccatggacct gtgaaaaaga aattcccttc
     5401 aataagcaga tctttccatc accgtctata attgactggt tttgacaaac aagacttgga
     5461 aaaggtgaga ttattcttgg acgatcatcg catgaccgcg cctgtagccg cggccctggt
     5521 gtcagtttat ttcttctggg ggaccacgta tctggccatg aaattcgcga tagagacctt
     5581 gccgcccttc attatgctgg gactcaggtt cactacggcg ggagcgatca tgttcgtttg
     5641 ggaatggata cgtctcagac cccggatctc ctggggacag tggaggggta tggccatggt
     5701 cggagccctg atgcttctgg gtgggaccgg gggagttgcc tggtcggaac aggtcgtgcc
     5761 ctcaaatatt gcagccatag ttgtggctac tgttccattg tggatagcca tcatccgctg
     5821 ggtctatctt cgacacgacc gcccggccag gatcaccatc atcggtctgg tgacaggttt
     5881 cggggggatc attatgcttg tggcttccac cagcggacct tccgcagcag aggggcacat
     5941 cacgggactt gtagtactcg gatttgcaac ttttctctgg gcttcaggtt cgctctattc
     6001 cagggtggcc gaccaaccgg actctccagc cctggccatt tccatgcaga tgcttaccgg
     6061 tgggttgatg aacctggtag tggggatagc ctggaacggt ggttccctgg taccgatgag
     6121 cgaagtgtcg ataaggtcgg cgactgccct cggatacctg gtcgtattcg gttccattgt
     6181 gggtttcagt tcatacatct ggcttctgaa gaagaccgac cctgcccttg cttcaagcta
     6241 tgcctacgtc aatcccgtcg tggcgataat cctgggctgg gttctggccg gcgaaagcct
     6301 caatgccggg agtcttgccg cgacggctgt cattctgctt ggagtggtaa tgatcgtaag
     6361 aggtacagtg agaccagcca tccgatcatc ctgagggcgt ttgaaagatc gatcaaaaaa
     6421 gcaaaagggc cgccttcccg ggcggccctt tctccttcct tgtaagtgaa ggctctactt
     6481 cccatccttg acaggcttct tgactattgg aggtcccggt tttttctgtc ctggagnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctg tatcttcggt ggtccaggat
     6661 tgttctggaa cttcggtggc gctggtttgt tctgtacctt cggcggtcca ggattgttct
     6721 gtatctttgg cggtgcgggt ttatcctgga actttggcgg cgctggtttg ttctggatca
     6781 cctttggagg tgacttgatc tcctgcagtt tcgggggaga tgtcctttca cggtcccatc
     6841 ggcggggttc cctttcactg atcattgaat cgaaggttct ccgctgctta tcgtcaagga
     6901 tcgacctgaa cctgtccctt gagtcaggtg ggggagtcag gctctcctgg cgacgcttga
     6961 gaaggtcacg cctgtacttg tcgatatccc ggaaggagtc ccggtcccag ttcctcttaa
     7021 ggtaatcctt ctcaaagtcc ctgtagcgtc tgtccctttc ccactgtcgg ttgtggagat
     7081 ccctgaaata acgctccata tcccgtcttt gcttttcgct caggcggagc cttctgtccc
     7141 agtccctcat catttcccag tagttccgtt cgtaatcccg cctgtgatcc cgcctgaagt
     7201 aatagtcgtc gtaccagcgg tcgaagtata ccgtgaaagt gcggcgctgg ttcatgtcca
     7261 ggatcctgaa gaacaggtag aggaaatcgt cgatccttat ccactggccc gaaccgtatc
     7321 cataatcata cccgtagccg gggtcgtaat acctgtcatt gtcctgggcc tggctgataa
     7381 gtattccgaa ggccaggacc gcaaggaagg catccaggtt gttgctacct ccccgctcta
     7441 ttacgaccct ggaacctccg ggccttatca tcctgccaag gctgaacagg gcatcaaggt
     7501 ccctggagta gcgatgatcc tgcctgtacc cccagtccct gcttctaagc tcctggaagg
     7561 agttcctctg accgggtgta aggttaaggg tcctcatcag gtcaggagag aagaggctgt
     7621 tcacctcgtc ctcggaccag tagactactc cgtccaggtc tggctcccct gcagaagctg
     7681 caacggagag acccgtgcca aggaacaacg accccagaac aagcaaggca acgatctttt
     7741 tcatggggtg tacctccgtt tcttaaaagg tgatccctct tgtctttcaa atcctgttcc
     7801 aatcattatt ctaggatcga ctccattaag gtgctctttt gaaatgtaaa gaattgtata
     7861 gagccccgat ccgtcatgat agatgggagc tcccttggtc aagggacagg atgttattcc
     7921 atatcgctca gggcgtgtgc cctggcttct tcagagaggg tccggccttt ttctgaggct
     7981 gcgccgatgg ccttcatgaa agcggtgcgg atccggctct cttccagttc atatatcccg
     8041 tcgatggtaa ctcctcccgg ggtcaggacc atgtccctca actcggaggg gtgcttccct
     8101 gtttccagga ggagacagga tgccccgata gcagtatatg ctgcggcctt tagtgccaaa
     8161 ttccgtggaa gtccctccct gagggctcca tatatcatgg actccaccac ggtgaagata
     8221 taggctggtc ccgaaccgga cattgccgta aggacatcca gatactgctc ctccacctgc
     8281 tggatctcac cgaggttcaa aagaaccttt tcgaggtctg gaaacctctc ctttgagaga
     8341 tcttgctgga gtgaaaacag ggtgtaaccc ttccttaccc tgacggcgat gttggtcata
     8401 gcccttacga agtgtgcttc gggcgctgcc atgtggagga tgtccgtcga tatggctgcg
     8461 gcgaaggaaa ctacggtctt tcccctgacc gcttcagcga tctccttgag gacaggcaca
     8521 acctgcaccg gttttacgga gatgataacc aggtcagctt cctcggcggc agcccggttg
     8581 tccgaggtga cctttatacc tttcccggca agtccgttga tcttgtcaca gttccggcga
     8641 gtggctgtta cctcgtattg tggggtaagt gcttctgcaa tggcccctcc tatgattcct
     8701 gcaccaatta cgcagatcct ggtcttcgat ctctcttcca cgatattccc tccttgaaag
     8761 aaatagaaaa aagggccgcc gacccggagg tcggcggccc tgaatgcctt atctgtactg
     8821 gatgcatcgc aatatcagga catgaccgaa ctttcccggg aaagtgtctt tcgggacctg
     8881 ggtggaacac acgggttcgg tcgggtcgtc cttttcaaag aagcctgcat gatctttctc
     8941 ctgaacagtc tttgaagcaa caattgacga agaggataga ccctgtcctg tctctcgtca
     9001 ataccaggct tgataaaaca gggttcgaga ggttaatctt ccttccggga ggggtcctga
     9061 tggcgaggtg gctaattgtt gccggtggtt tcctgatctt gctggggctg gtccttcact
     9121 ttgctccctg gcttttgaca tgggtcggga ggcttccggg agacataagg ttcgagacag
     9181 acaggacaag ggtctttgtg cccataactt cgatgatcat tatcagtgtg atcctgacgc
     9241 tggtggtcaa tttgttgtcc agaaggtgac cggtgaagat gtaaatcctg ttcgggccag
     9301 atgggcctaa catcttttat tgatactgaa cgccagagac ctgcaccagt cgaggaagat
     9361 gccgtatggg cgtcctgcca gggagaagat cagcgtcatg aacaccgacg atacagctat
     9421 ctgcctccag gttgcgccgg caaaaagcag cactattacg taaagaggca cctggaatat
     9481 gacaaaggcg agggtgtcac cgaagatcat cttcaggggc cgggattggt ctatctccag
     9541 gaaggcgaaa agcttgtccc ggaaccagcc gtaaggcctt ccagtgacca ggttgacggg
     9601 tatggcggcc atccgtgcct gcagggactg agaaaccgtc agctttgcaa tgaacacctc
     9661 gatcatcatg caaagggcag tggagaagat gatcatggcc gcgatgtcag ccaccgccat
     9721 gatgatcctg ttccgtccct cttccgggat ttttgctgct tttggcaaag gttctccccc
     9781 ttttgagaga tcgaaggttg ggccccctga atgctactgg atcttcggta ctttttaaaa
     9841 acggcatggg tgtgttcctg ttcagtaaat actaaatgaa actggtctga agaaccagct
     9901 tgcttgcaaa tacattggcc caaaggttat ttcatatact tgaacctggt gctatgataa
     9961 ccaggtaaac attctttgtt ataaaaagaa aggggttggg gaagttggca aaaaggttgc
    10021 ttttcccgtt gactgtcctt ctcttgcttc ttgtctcggc caatgctgtc caggctcagg
    10081 acatctccgg cacttgggat gtaaaggttc acggttttaa ctattttacc gacagggagc
    10141 cttcccggat ggcgatctct gagacctcca caaccatgga gatagaacag gaaggagaga
    10201 gagtctcaat aaccctgggt gctttcggtg gggtccacgc ggctaccata ttcaaaggtg
    10261 tagtgggtaa tgaccgattt gtggcgagct ggtggtacga gggttaccct tacgagacca
    10321 aggtccttac gggacagtac tcacctgaca gcggaaggat caaggggaaa atgatctacc
    10381 cgagagctgc cgacaagccg ggtctggtgc cgggctggct tgaggtcgaa ttcctggcga
    10441 acagaaagat caccctgatc ccgaaacccc tggaaccggt ccttgccggg gcaatccccc
    10501 ttcttctgcc ggaagactgt ctttccctga gtccgtccaa tgtccaggtc aagaatattg
    10561 gcggaaggtg gaagatcgtc gatggagacc actggatcct ggattttgga aacaagaaac
    10621 ctgaggccga caaggcgctg aatatcatct ggagctacgg actggacaag gcatgctacg
    10681 ttgggcgtcc ggatccctcg atgacctatt ggacagcagg aggccaggcc ccttcaggtt
    10741 ccctctccgg cgaggactgt ataggtttca accctgggac catcgaggtc aagaatgtcg
    10801 acggtcgctg gaagatagtg gacggctcgc actgggtact cgatttcgcc tccagccggg
    10861 aagaagctga gacagcactg cgtatcatta agcattacgg cttccggtat atttgttttg
    10921 tcggaaggcc cgatccttca atgacgtatt tccgaaagta aaaccccaac cggaaatgta
    10981 aaagaaaagc gacgggagga cccgtcgctt ttcttttttg gaagaccgaa atgactcagg
    11041 agttgatgtt cattctgcga gccatttcga gagctctctc ctccaggtca ggcccttcca
    11101 gttctgccac cacatccttc aacagaaggg gaatatccag accctggggg catttcttca
    11161 agcatttccc gcactctacg cactgggagg cgtagttgct ctctcctctt cgtgtgatcc
    11221 cgctcatctt caccacgtaa gagttcttcc cctcctcgga gtttccgaag agatgcatct
    11281 tgttgtagac ctcgaaacag gaggggatat tcacacccgc aggacagggc atgcagtatc
    11341 cgcatccgga acagttgacc ttcatgatat ccctgtattt gtcggcagcc cttttcacca
    11401 gacttttctc ttctacggtc agcgagtcgg gaagagcctc actggctatc ttcaggttct
    11461 cctctatatg ggattcctcg ttcatacccg acaagaccac aaccacctcg gggtggtccc
    11521 agacccaacg aaaggcccac tccacgggag tcctctttac cggggcttct tcccatatgg
    11581 cagccacggg tggaggtgct tcagggaggc ccaggtttcc cccacggagc ggctccatta
    11641 caaagacccc cagacccttt gaagcggcgt atttcaggcc ttttgttccg gcctggtttt
    11701 cctcgtccag gtagttgtac tggataaggc agaattccca accataggaa tccactatag
    11761 gggcaaaatc tgcaggaagg ccgtgaaagg agaaacctgc gttcactatc ctcccgtctg
    11821 acctcgcctt gtcaaggaag tcccttgcgc ccatggtatc cacgcaattc cacagggggc
    11881 cggtcagagt gtgcataagg tagtagtcta tgtggtctgt ctgaagtttt tccaactggg
    11941 cgtcaaggaa ccggtccata tcttcccggg atctcataag gtaggagggg agtttggtcg
    12001 ccagtttaac tctctccctg taaccgtcct tcagtgcctt cccgaggaag gtctcgctct
    12061 ctcctccatg ataggtccag gccgtgtcga tatagttgac gcccctgtcg atggcgtagt
    12121 gtatctgggc ggtggcacgt tcctcgtcga tacggccgtc cttgacgggg agccgcatgc
    12181 acccgaatcc cagaattgac agttcgtctc cgctctttgg cactttccgg taaagcattc
    12241 aaggatcacc tctcctgcat cactttccca ctggaagttg tcctgggatc gttcaacccc
    12301 tggggtcagc ctcgcagtga ttcttcaggg tccccaggcc ttcgatctcc acttccacaa
    12361 cgtcaccggc cttcattgga cctatccccg ccggggtccc agttgctatt acgtctcccg
    12421 gcatgagggt ggaaaagcgg gtgatgtagg agattatccg gggaacggga aaacccatca
    12481 tgtcagtgtt cgaggactgc accaccttgt cgttgagcct ggtctcgatc ttcctccctt
    12541 tcgtctcact gccggtaacg atccagggcc ccatagggca gaaggtgtcg aaactcttcg
    12601 agcgggtcaa gtgagcgtct ttcttctgca gatcccgggc ggtaacgtcg ttgaggcagg
    12661 tcaaaccgaa gatcacgcct ggggcttctg cttcggagac gttcctgcac ctcttgccga
    12721 tgaccacggc aagctccccc tcgtaatcca ctcttccagc ccagccaggg ataaggatcg
    12781 tcccttcgtg ttccaggagg gagttggtac ccttgaggaa gatatgaggt tcctcgggca
    12841 gttcgctccc cgattccttc gcgtggtcct cgtagttcaa gcctacgcag aaaatgtttg
    12901 ccggctcggt cggaggaaga aggcatactt cttcaagcag agggtactcc cccgacagct
    12961 ctcttgtgtt ccaaggagcc cctgtcaggg cggatatcac gttgccctcc agtatgcccc
    13021 agcctgtttg cccatccttc tggtacttga tgtatttcat ggacacttcc tccctttctc
    13081 ccggctgcca gatcgttcac cggcccgccg tcattcttcg gaaatgcttc gccgtctctc
    13141 gcctgaggtt tcggggttgc ttgatgaact cctccagtat aaccaattgg tctggaaagt
    13201 ggcgacggta tgtgcccctg agacgttcga tcactgcagg gtaggggttg aggggatcaa
    13261 aacccacatg cctgataccc ttttctccga gtgctgcaag gagttccaga acagagtcct
    13321 tcctgtcggt aagtcctggc aaaagagggg caacgaagac ccagacaggt ataccctctt
    13381 tcatgagctc ttccgcggtt tcaatgcgaa gcagcgggga agaggccccc ggctccatta
    13441 tccttgcgat cccttcatcc agtgtggtta tagtgaagcc aacctcgcag cctttcaact
    13501 ttctcaggac cggaatatcc cgtcggacca gggctgactt ggtcagcacg tggacctcca
    13561 tccggggatg tcccgcaagg atctcaaggc ttgagcgggt taaccggtat ctgctttctg
    13621 caggctgata agcgtctgta acagtcccca ggaggactct ccccgacggg gttgtccttg
    13681 aacgctccag ctgccgtgcc aggatcgacg ggaagttagc cttcacgtcc agatattctc
    13741 cccacttctc ctcgtgcccc gaaaaccggc acatgaatga ggcataacag tagacacagc
    13801 cgtgagtaca acctccgtaa gggttcatgc agtagtcgaa gcctggtatt ttggtcctgt
    13861 taagcgcaga cctgcagtta acatcccgga ctttcacacc ggaaatgctc ctctcagggc
    13921 tttcctccgc tgtatggtcg ttatcgaaga gggtaccctg tcccaatgat cccgtgcctg
    13981 gtcccctgac tcgatcgttc ttcattctct gttctcctca atcgttactc gcggtactac
    14041 cctgccgcca gtaacgctat ccctgccaga atgatcgcag atcctgtcat cctgcgtttc
    14101 ttgtacccct ccccgagtac tgttgcccca agaaatgtac cgatcacgat acttgcctct
    14161 ctggctggtg cgataaggct caggggggtg atagaaagag catacagcac ggccaggtag
    14221 gcagaggtgg ccaggaaggc tatggtaaaa gccggtcttc tgtaataagt ccactgtaac
    14281 aagatccctt cgctctctcg aagagcaaga ggcgtcagga acatgacctg aattactatg
    14341 acagcaaaga agaagagcgt cggcggaaag cctctttccc cgacagccat cctgtccact
    14401 agggaatacc cggcaatgca tactcccgtc gcaatgccaa gaagtggtgc agtccttgaa
    14461 cggaagtcct tcaggtgggg agaaaatccg gcgatccaga ggatgccagc tacgacaagg
    14521 cccgttcccg ccaggttaag aggggtcggc ctttcgccca ggacaacgat ggccagaaaa
    14581 gttgctacca ggggcccggt tccccttgca agggggtaga tcacagacat gtcgcctgct
    14641 ttgtatcctc tctggagaaa gagaaaatag ccggtttcga agacagcgct ccccgaggcg
    14701 aaaagaagca gttccgtgcc caagaaacac cccttccccg atgtggcgga gagagcgaag
    14761 gggaacagca gcaaaacaga gaaagacgag aagagccatg tgaatgctgc gccgccggcg
    14821 acccgcttgg cgaccaggtt ccatcccgca tgcagaaatg ccgcgataac gagaagggac
    14881 aaggataaca gggtcatgaa cacctcaggt tttgggtcgt tattcctaaa tcaaaagatc
    14941 aaacgcgtac tctggcgcta cccctcgaaa atcgactcgg gattatgcgc ttggggcaga
    15001 gcgaagccta ccctccaaac aagctgaagg acctggtccc aaggagtgtt aacgaggggc
    15061 acgccctttc gcttcgctct tggcacctcg tcccgacata gtcccgacaa ggtcccgagg
    15121 tgctttaacg agggaggtgc tttaaagagg ggatcgtcgg tccaatgagg gaccttgtaa
    15181 acggtaaaac agtcaacggt aaaccggaga gtcatacaga tcttttgttc cggacttgtc
    15241 ctaaacgttc accgttgacg cttttggtct ttgcattttc taacgtcttg cgaattacct
    15301 actccacccc tgcctcgatc acagtaagcg tcgatcttga gccgtccagc acagccctga
    15361 cgcccagggg tatcgtcgcc ttctttacgt cgtgcccgaa acacccgtta ataagggtcg
    15421 gcctccccac cggcgcaacg accgcctcca ggacctcctg cagggtgaag gagtccttcg
    15481 gctttctagg ctggatattg gtgaactggc ccagcacgat ccccgccgcc gcatccagtt
    15541 ttcccgccag cctcaactgg gtcagcatcc tgtcgatgcg gtagacgggc tcgttggtgt
    15601 cctccagcag aaggatcttc ccccttgtat cgatctccca gggtgtccca aggagtgagc
    15661 agacgagggc aaggttcccc ccgatcgttt ctccttcggc agttcccggg acgaggggct
    15721 ccataggggg cgcactttcg tagggtacga tctctcccag aggttcggtg ctggtaacag
    15781 ccctgagaag actgtccctt acatacccag ggaaggagtc gcgaacaaac tcgctggcgg
    15841 gcatcggacc gtgaaaggta atgaaccccc ggggtctgtt gagcatcagg tgaaggatcg
    15901 ttatgtcgct gtatccgaga aagaccttgg ggtttctgta gacagcatcg aggtccaccc
    15961 tctgagggag ccggatggcg ccatctcctc cgcgaacgca gaagatgccg ctgacgtcgg
    16021 ggtccatgaa catcctgttc aggtcatcgg cccttacgaa gtccggtccg gagtgataac
    16081 cctcggattc gtagcagctg cgggttatct tgaccttgaa ccccatgtcc tcagtgatct
    16141 tggcggcttt ctcgatcgaa tccatcgaag actggcttga aggggagatg aaagccactg
    16201 tatccccctc tctcagggct gggacttttc gcatgggaga cactccttcc agcttaaatt
    16261 aacctctatt tactgatttt atgagcatcg tagcatatga tcctgctggc aagatgaact
    16321 ccagggatat gatccgtctg ccggggaaga ggcggtcggg ggcttcttcc catttgaact
    16381 cctccggcac tgatattgcc ctgcgtccga aggacgagag gaaggcctgg tggatctctg
    16441 gcagacgaag tttggagagc ctcactccac ggctgtagag aaccctggag agcacctttc
    16501 cgaatcctcc ggggatctct ggcatccctg ccgccggtgt gggaatgacc agctctgcca
    16561 gtctcctgaa agcttcccgt gtaagatcac ggtactgcaa cagatccaca ggacggcacc
    16621 tgatcgtgaa gaggttcccc acggtctctt tgagaatacc ttcaagaaga aggttccaaa
    16681 ggaagctctg gtaggaggag aggtactggg acatctcctc ccggggaagt tttccgagcg
    16741 cctgcagttc gctcccaccg cgggtcaata cctcaagcat cccgcggagt cgaggttcag
    16801 ggcagagtgg ggacagaacc ttccagttcc cccactttga ggcgatctcg gcctttcttg
    16861 cctttaccct ttcaggagcc ttggggtgga aggttgtgaa gatgcacttc agagcctctc
    16921 cgtatcgccg cttgacgatc atctctcccg ccaaaacccc tccatcggat acgtccccga
    16981 agcgttgatc gtcgaagtag ttggcaaaac caaagagttt tgtctcctcc aggcgtttgt
    17041 cgataccttc agactcctga gcgtccaggt ccctgagcac tatacggaag cggttccctg
    17101 aaatggaccc tggtccaagt ctcgaatcgg agaagcccgc gaactctgcc gaaaggttca
    17161 tgcttcgaaa ggtcatgtcc acctgtgata acgtagaaaa atactgttct gtcagcgcag
    17221 acctgtcctt ccttccacac cagtgaaggt ccctgagttc cacatccctg gaacgggcta
    17281 tctccgccag tgcgtccaag gtgttccagt tgcgcttccg aagcctgtaa acccgatggg
    17341 cggcaggtgt ctggaccagc ggcctggcga gtatctcttc cacctggaaa tcttcggggt
    17401 tgacctttat cttcaattct ggcctccagc aggggcatct ttctgcaatt acaaggataa
    17461 tggaaaggtc tcccgatatc acctgccggt attataattg tgctcatcgg aagggaggat
    17521 ctcctatgct gttcgatccg ggacttgtcc aggtctacac tggtaacggc aaggggaaga
    17581 ccactgctgc tctcggtctt tcactcaggg tctgcggcca cggcgggaag gtggcgttca
    17641 tccagttcat gaaagggtgg gatttctacg gtgagatcgc tggtctcaaa cttcttcagg
    17701 gagtccgtca tgaacggacc gggactgccg actatgtcaa ccggggaagc gagagcgaag
    17761 tggaccgcag tgaggccgcg aagggggtat ctctggcatt ggagtgcctg tcatcgggtt
    17821 cctatgacct tgtagtgctg gatgagataa atgtggctgt cgactacggg atggtggacc
    17881 ctgaagaggt ggctcaagcc atcattggaa ggcctcgcca cgttgaggtt gtcctcacag
    17941 ggagagctgc ccccaaagcc tttctcgatt tggccgacct tgtgacggag atgagggagg
    18001 ttcgacatcc ctactcaaaa ggtatcagat ctcgccgagg cgccgatttt taacctggat
    18061 ttgttcgcca ggttttttca caaatgaacc ctttcaatgt ttctcttttc catcaggact
    18121 ctacccaaaa gatttaatat ttgctagaat tgctcaacgg cttttatctg gtattgataa
    18181 tcaaaatcaa aagccgaaat tttttaggag gtagagtgag gtgtccaagg agaaacagga
    18241 tctgttgtca ttcggagcac ttgcggtcat ttcggcagcc ctcttttcgg ggcattttgg
    18301 tgtgggtgat gttatctttc ccccgatcct tggcaaggga tccggtgcgt cctggttcac
    18361 tgcagcaatg ggctacggga tcatcaacag tctgggggtc cttgtggcct accttgcggt
    18421 agcacgacag caaaagacac ttctcgagat gagttcccga accctgggaa gggcttttgg
    18481 agttgttttc acaactatct gcatgctgat catcggtccc gtctttatcc ttccgcgcgt
    18541 ttcctcggcc acccacgaga tggccgtggc tcttttcttc cccaacttcc ccctggtggg
    18601 gaccctggcg gtcttctttg cacttaacta ctgggtggcc ttcaacaggg cccaggtcat
    18661 cgacaggctc ggaaagatcc tctcaccggc gttgataatt ttcatggcgg tactgataat
    18721 caagggaatc gtttctccca tcggcccagt accggcgaca ggctccggaa acccacttgc
    18781 cgacggtgtc cttaacggat acaacaccat gaatgccctc ggagcggccc tgttcggggg
    18841 ctggatcctc aaggagctcc atttgagagg gatcactgat aaagatgccc agacccgaaa
    18901 tctctcctac attggccccg cagttgcact gggacttttg gtcacgtcca cgggcatcac
    18961 ctaccttggc gccacggcca gcaccatttt ccccgatgcg ggaatagggg tctacaccgt
    19021 catgatcgca gagggagtac tgggggctgt aggaaaggcc gttttcgcgc tgcttcttgc
    19081 attcgcctgc ttcaccacct ccgtcggtct gacttccacg gcaggtgatg tcttccagga
    19141 gatgtccggg ggcaaactgc agtacaagac cattgttgct gtctcgagcc tcgtcggctt
    19201 tggcatcggg acggtagggc tcggcaagat cgtaggttac accgtaccct ggcttatgct
    19261 ggtctatccg gctattattg ttctgctggt gggaagcctt tttgacttca gcaagtacaa
    19321 gccggccctc cagggtgcca ttattacagc ggtcatcttg agcatcggtg attttctcgg
    19381 tggcatgggc atggctgaca atcctttcag tgccatgacg gcgaagcttc cccttggcgg
    19441 ccaggggttg ggttggcttg tgccggcgct tgtggtcggt gctttggcaa tgctcctgcc
    19501 aggaaggtca aaaaagtaac accctaggag gtctgataat atgcagttca ggggagagct
    19561 tgattcagcc cggatagtga ccgtccttga cgactacgcc gggtacgaga catcctttct
    19621 ggcccagcac ggtatagcca tactcctgga ggcttcttcc ggaggggtgt gcaaaagggt
    19681 acttctggac gcgggacagt cggcgctgcc gatactgcat aaccttgaga taatggacat
    19741 cgatcccggc tcgatcgatg ccattgtcgt ctcccactgt cattacgacc acactgaggg
    19801 ccttgttgag gtcctgaggg ccgtaggccg ggagggcatt cccgtcattg ctcacccgac
    19861 gatgttccgc cccaactaca tctttaaacc ctttatccgg aatattggcg tcaccggaaa
    19921 gaacggtaag gtcgccatcg aggaggccgg ggggcttctg gtcctcgtcg acgaaccctt
    19981 cgagataatg ccgggagtga ttgttacagg ggaggtcccc cggagaaggg cctttgagaa
    20041 gcagggaata gggacctaca acatcgaggg gggcaaggtc gttcccgacg agatacgaga
    20101 cgatctctcc ctgaccgtca aggtcaaggg gaaggggttg gccgttgtct ccggctgcag
    20161 ccacgccgga atagtcaaca taatcgactg gtgcagggag atcgccgccg agcccagagt
    20221 ggacctggtg ataggtggct tccaccttat cgaggcctct ggggagagga tcagggagac
    20281 tgcgcaggct ttgaagcagc tgaacgtcgg aaggatcgtg acgggccact gcacaggtct
    20341 ggaggccctc tgcgaattct ccaggatcct cggggatcgc ttcgaacagc tgcacgtggg
    20401 gaccacgatc cagctgtaga tccaggaaat tttccagggc ggcgccctta aggggtaccg
    20461 cccttcttcc ggaaaggaag agtctgagat gatcaaagga tgcctggctg tctttttgtc
    20521 tgcaagcctt tttttcatga ctgtttcagc ggggtttgcc tgcagtgagt acgaccccct
    20581 ggtggggggc ctggaacggg aaggggtcat tgaggccctt cagggggaac ttctggaaag
    20641 tttcggcata gaggccgaat ttgtcttcaa ctggttcggt atcgaggatg tttgggcgtg
    20701 ggtggagagc gaaccacggt ctccggacgg actgaacagg tacgagccct tccttgccct
    20761 cttgaagaag gagggcgaaa actgggccgt cactgaggtc ccccctctgg aggatggctc
    20821 ccctcccgtt gatgatgcat atttcaggag cctgatggag agatatccag gcctgccgga
    20881 agggatcttc ccctgggaga gatagagggc ctaaatgtct tcggcggctc tggccctctg
    20941 accatcgctg aggtcaaggc ttgcgtcccg aacgaccacg tcgccctctt ccagcccctc
    21001 gagtatctcc accatcctgc ggtcggtgat gccaatcctg acctcggttg catggatctt
    21061 cccgccctgg agttggaaga ccttcctgat cccgtcttcg gtacgaacag actccaccgg
    21121 cagcagcagc acgtcctccc tgcgagacgt ctctatgaag accctgacct cgtagccggg
    21181 tttcaaaacc tccgacatgg ggaaagcgat aatgaccggc acccttctct gggccacccc
    21241 gagaggggag accttctcct ccgccagggg atagatcttc ttgacatgac cctccagtgt
    21301 aaggctgccc agaatggggg ctgttatcct caccctgctc ccgaccttca ctcctccaag
    21361 ggagtcgctg agaacgtctg agcgtatctc catttcgtcc tcaatcccga gagcgaccag
    21421 aagctctcct gccatgacca cctgctcctt cttgaccggg aggtcgagga catagccgtc
    21481 gatcgggctt ttcagtgtca attcggcctg tttcttctcc agttccagga cctgttgtcg
    21541 aagtcctctg ctcagtgccg aggccgaccc cagcctggcc tcggcctcgg caaggtcctt
    21601 ggtgtatcgc tctactctca ggctgacctg ctccatttcg gtcattgaaa gggctcctgc
    21661 ctcgtagagt tttctgtagc gctccatgtt tcgattctca tccgccagaa gaaggcgaag
    21721 gctcgctgcc gcagctgcat agccgatctc gctcttctcg gcctgtctcc agtatgccct
    21781 cgtctcggcg aggatcgcct ccagatcagg gtttgtcatg cctatgagca actggccctg
    21841 cgagacctcg tctcccgcct ctacggggag ttcagttatc ctagcggtct gggttgcgta
    21901 gaagttcctg tcctccaaag gctgaaggta gccgtcctcc tcgaccccgc ttgatatctc
    21961 ccctctgact accctgaccg cagccatctt ctcgggcctg aggaggaaga agagtgtgac
    22021 agccagcagg ataatgatcc cggaagtcgc taagatcttc agtcttctct ttttcatggt
    22081 ccatacctcc tcagtcgtta gccttcagag tctccaccgg ctggagtctg ccgacgcttc
    22141 ttgccgcaag accccaggag acgaggatga acagccctcc gcccaggacg gagatggcgt
    22201 aggtagtggg gtagaccacc gcctcgaagg tgaagaggtc ggtgctgact ccgatcgcgt
    22261 aagcctcggc catgagcctg ccaaagggga gtcccagcac ggcccccacg gcaagaagga
    22321 gaaggttctc cttccatagc agcgatgaga cctctctcgc cgagaatccc agcactctca
    22381 ggcttgccag ttccctccgc cgttcggaga aggagaggga agacgcgttg aagactatgg
    22441 caaagcccag cagcacggag aagaaggtca ttatcccgac agaatagtaa aggtatttca
    22501 tgttcttttc gaggaaccgt acctctttct ccctgctggt cacggagatt acgttgatca
    22561 tgtctcccag tgcatattcg atgctttctt tgaactcgtc ctgggtgttg aagacaatgc
    22621 ccgaggcgat cccctgttcg ttcagcagac ggttggcagt atcaaggctt acgctggatg
    22681 cgctcccgaa ggccttgcgg ctgatgcccg ccaccctcag catgctcttt ctgggtgggc
    22741 ccagcccaag gatggtctca acctccactt cgtcacccgt ccccgcgccc agaattctcc
    22801 cggtgtacca gtccaaaaac aggccctcct cgggaaaatc cagttcatgc ccatcccggg
    22861 cgataaccct gccaaggccg gatcccgtgt gcatggcgaa gaggacatcc tccctgtacc
    22921 gccccttgta gtggatcctt accgggagct ctaatatggg ttcggcctcc cttacccccg
    22981 gaaggttcga tatggacagc agatccttct ctttcacggg gttgaccact cttaccagga
    23041 agtcgaatcg ccactccttt tcgaagtgtc tctccatgag gtcgaacatg gaatccctgg
    23101 caaaaattga gatgactatc atccctacgg caaggaggag ccccaggatc gtgacggccg
    23161 accggaatct gttcctgaac atggagcgca ggctcatacg ccagctcagt cccaggtgtt
    23221 ccattagtcc agggatattc tcgatcatac tcgggcggaa ggatcgtgga gtggccggtc
    23281 tcatggcctc tgcggggctt accgccagca ctcccctcgc ggcccagaca ccggccaggg
    23341 tccctacgac gagggttgtc ccgaaaacca gtcccgccgc cctgaagtgg agagcttcct
    23401 ccaggcgtgg caggttgaag aacatcatgt atagcttggt catcagacct gcaaagaaat
    23461 agccaagacc ggttccggcc accgcacctg cagaggcggc cgaaagggcg taccctccgt
    23521 aaagggccag aagtgaaacg ttgccgtacc ccactgcctt cattattcct atctggcccc
    23581 tctggacctg taccatcctc ccgataaaga cgaactgcat cagaaccgct atacccagga
    23641 atatcgtggg gaggaacctt gcgaaagcct taagctggtt gagctcccct ctgagaatag
    23701 cctcgctgat ctggtccttc tgttcgtagt cggccaggct gccatagggt tcaaggatgt
    23761 tcctgatctc ccttgctact ccaactctgt ccgctccggg gatgaaacgg accagtacct
    23821 ggttgacaga acctttctcg cccaatatct gctgtaactg gttctggggc aggactgcca
    23881 caccgaagga ggatgggtct tcaagcatgg acgcggcgtc cttcactgcg tagacgaaat
    23941 cgggacccgc cgccgttcct gttatggtaa gggtcttcct tctcccctcg gctgctatgt
    24001 ctatagtatc tccctgcctg aaaccatggg cctcggcgaa ctgcctgttc accaggatct
    24061 ccaccgcccc gccctcgggg tacttctcgg ccatcctgcc cgagaggact ttgaccctgt
    24121 taagttcctg gcggtcgggc agcggataac caaggagtcg gatcgtggtc ctttcatcct
    24181 cgttatttat taccggcacg tccttctgga tcctcacaga ggcccggagg acccctggga
    24241 ctgcctccac gcgcgaaagc accccctcgg gagccctgac gacgtggaag aagtgatctg
    24301 caaagtccgt ctcccggtag aaggaatccc tggaccttat caggttgtca gacatggcgg
    24361 tcattgtcac gaaagccatt actcccgtgg cgatgaccac tgccgatgcg atgaactgac
    24421 ccagggtcga gcctatggtt ctgagaagtt ttctggccag gaccggcatc accaggagat
    24481 cctctccggc ggaagtggtt cctcgttgta tgtttcctca acgatcctgc cgctgctcat
    24541 cctgacaacc cggcgggcca tatcagctat ggaagcgttg tgggtaacta tcaccacggt
    24601 ggtcccgaac ttcctgttta tctcttccag ctgtgcaagg atcagccttc ctgtggacgc
    24661 gtcgagagag cctgtcggct cgtcgcagag caatagcagc gggttcttca caagggccct
    24721 ggctatagcg acgcgctgct gttcgccgcc gctcatctgg gaggggaagt ggtcaatcct
    24781 gtcggacatg cctacaccgt caagcacgtc ttcggtgggg aaaggggagt caacgagttc
    24841 cgcggcgatg tcgacgttct ccctggctgt cagatcgggg atcaggttga agaactggaa
    24901 tacgaaaccc accacgttac gtcggtagag ggtaagctgc ctgtcactgg cggcggagag
    24961 gttctcatcc ctgaaccaga cgtccccgcc agaggggacg tccatcccgc caagcaggtt
    25021 gaggagtgtg ctcttgccgg atccgctggg gcccaggatg acaaccagtt ccccctcggg
    25081 gatcgagagc gatgattccc tgagagcctc cacttttact tcacccatgt cataggtctt
    25141 gaaaaccctg tcgagcctcc aggtcggccc cttttcctgc atgaaaaata ccccccggat
    25201 gctgtgaagt atacgttctg tcgtctaaga aggtcaaacc gggcgtcggt tttactgtaa
    25261 aatcagacaa tgatcgtcag acaaacttca atgggtagca tgggaagaag ctgcggtgtt
    25321 ctcctccaca tatcctccct gccctcccgg tggggcatag gtgatttggg cagaggggcc
    25381 gatctgttcc tggacttcct ctgctcttca cggcagtccc tgtggcagat actccccctt
    25441 tcccctacgg atcctggcca gggcaactcc ccctacagca gtttctctgc ctttgggggg
    25501 aatccgcttt tcatcagccc ggaactccta gtcaaggatg gattgctcga gctgtcagat
    25561 ctcgattcac atgaaccttt gtccatgaag gcctgcgatc atgccggctc tgcccggata
    25621 aagaggagcc tcttacagag ggcctataaa aggttcgcat cctctgagga cagcgaatac
    25681 tacctgttct gtgagaagaa cggtcactgg ctcgatgatc ttgccctttt cttatccctt
    25741 aaaagacacc ttggcggagt gccatggtgc gaatggcccg cacccctgag ggacagggag
    25801 gagaaggccc tcgccgaggc cagggagcaa ctggccgaca agatagggta tgcccgtttc
    25861 cttcagtatg tttttgaaag gcagtgggga cggcttaaaa gagtctgccg ggagaaaggc
    25921 atttctgtca taggggacgt cccggtctac gtcagccacg acagtgcaga cgtttgggcc
    25981 caccgcgaac tgttcgatct tgaccccacc ggcagagcaa aaaaagtagc aggggtccct
    26041 ccggactact tctcggaaag tggtcagcgg tggggcaacc cagtctaccg gtgggatatg
    26101 ctggaaaaag agggcttttg gtggtggatc cggaggcttt ccaacgccct ttccctgttt
    26161 gacatggtca ggatcgatca cttccggggc ctggtcaagt tctggtccat cccttcctct
    26221 gaaaggtctg ctatagatgg agaatgggta gaagcttcac cggatgcttt ttttctgagg
    26281 gtgaaggaga atttcccagc catgcccttt attgccgagg atctgggata cattacacct
    26341 gatgtcaagg attacattcg ccgattaggg atcccaaaga ctctggtcct gcaatttgcc
    26401 ttcggtgatg atctcgaaaa gagtccttac gctccggcga atcacattga gaacgcctgt
    26461 gtctataccg gcacccacga caacaatacg acccggggtt ggttcgagtc cgaagctaca
    26521 ccgttgatga gaagccaact gaatgagatc gccgggcaca aggtaactcc cgaaacggtc
    26581 tcgatggata tgatatccat agctatggct tcgaaggctt ccatgacggt atacccgatg
    26641 caggatgttc ttggtcttgg ccaggaggct cggctaaata ccccgggaaa gccttcgggg
    26701 aactggctgt ggaggctgga agagaggcag ctggaggacg ctcctgtaga aaaactggcc
    26761 gaaatgacgg cccgatttgg gagagaggcc cgttagggca ttttcttgcc agatgagata
    26821 ataaataaca ggggaaagag aaaaggcagg gaggtagtgt agtaatgccg aagatgaact
    26881 acggcaacga cattggacat tcgctccgct tcatgatgga acaggacccg gctttccgaa
    26941 cagtggcgta cttctcgatg gaggtgggcc tgaaggcatc cataccaacc tactccggtg
    27001 gcctgggtgt acttgccggg gacattctga agagtgctgc cgacctgggt gttcccattg
    27061 tcggagtaac actactttac cgaaagggat acttcaggca gtccttcgag gactgtctcc
    27121 agaaggcatt gccggtggag tgggatccgg aaaagcaact cgccctcctg ccccacgaag
    27181 tgaccgtaat gatagaggac cgcgtagtaa aggtcagggc atggtgtctt gaactccagg
    27241 gcaggacagg gttcaccgtc ccaatatatt tcctggatac cgatgtgggg ggcaactctc
    27301 ctgcagacag ggagctcaca tggtatctct acggggggga cgaaaggtac aggctctgcc
    27361 aggagatcat cctgggctct ggaggtctga ggatgctccg cgacctgggc tactccaaca
    27421 tcgacgacta tcacctcaac gaggggcatg ccgcgttcct cgcactggag ctaatccggg
    27481 agatgggtta cgagaactac gacagggtaa gagagaaagg tatattcacc acccataccc
    27541 cggtgtcagc ggggcacgac cacttcagct gggaccttat aaaccgtgtt atggacagat
    27601 ccatggctgc ccggctccgc aggatgatgc ccaccgagga tgtctccatg acggagatag
    27661 cactccggta cagccgtttt ataaacggtg tctcggaaaa acatactaag gtaagcaggg
    27721 ccatgtacgg aagagaagac gtggactgca ttaccaacgg catacacacc ctgacctggg
    27781 tgagcccgga tttggcagag gtcttcacga aatatatccc cggatgggac aacgcaccag
    27841 aacggcttgt caaggctgtg cagatacccg tcgaggagat acgctccgcc catgcgccgg
    27901 ccaagaaaag gttgttggac tacgtggaag agaagacagg aaagaaactt gatcctgaaa
    27961 ggctgaccat cgggtttgca agaagggtgg cccagtacaa gagagccgac ctggttcttc
    28021 gcgatacgtc caggctggtc aaggccgcag ggggtaaggt gcagttcatc ttctcaggca
    28081 aggctcaccc caatgacaac cccgccaggg agatcctcag gaagctcctc tgcgaagccc
    28141 agaacatggt cgggatcgac atcccggtgg tcttcattga ggactacgac atggacaagg
    28201 cagccctgct cgtacagggt gttgatctct ggctcaacaa ccccgtccgc cccagagaag
    28261 catcagggac cagcggaatg aagtgtgctc ttaacggcat tcccaacttc tccgtactcg
    28321 acggctggtg ggtcgagggg tgcgtagagg gagtcacagg ttggagcata ggccccgaac
    28381 ctaaagagag catcagcgac cagtacgatg attccgttga cctcgacgac ctccttgaga
    28441 agcttgagaa ggtgatcatt cccacattct acgaaaggcc ccgggagtgg ggcgagatca
    28501 tgcgcggggc cattgccctt aatgccagct acttcaacac ccaccgcgtg gtccgtgagt
    28561 actgtgaaaa ggcctacggc atacagatga gagggttata aaaagatgaa gtgtgaaggg
    28621 tgaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttctt ttcgcactgc
    28741 tttccgccga tcatccagtt ctcaacgggc gactcctcga tcttgacggt taccgcctca
    28801 acagggcatc cgaccttctg aaccactacg tcggtaatgg cctttgccag ttccgccttg
    28861 aattcctgac ttcttccgga ccagaggtct actctcacga taggcacgct ttcacccccc
    28921 ttctccgatc attttccgta tatctgtgct taaaaggggc cggtaatttg aaatgtatct
    28981 tgaattataa caattaaact attactatag tatctgaaaa aaccgttgga gccaaagatc
    29041 tggccagacg tcgggcatgg aacgcagaga ggtaatgtct atagggcgtt gccaagtaac
    29101 gggggaattt ttccttgctg aaccgggact ctcaggatct tgcttccgaa tggatccttc
    29161 ttaaccagga agtcatgtcc cttcatgaaa agggacagta tgaacgagcg gcatcagtag
    29221 ctctcaggac tcttgaactg gccgatcggt tcggggaccc tgaccacccc taccttgcgg
    29281 ccagcctgaa caacctcgcc gggatctacg acgcccaggg gcgctaccta gaggctgagc
    29341 ccctatacca gagatcactt gagatacggg agagggtcaa gggctcggga cacgctgatg
    29401 tggccgccac actgaacaat ctggctgaac tctatcgtgc ccaggggcgt tacttcgagg
    29461 ctgaacccct ctacagaagg tccataagga tcctggagaa tgtaatgggc ccggaccatc
    29521 ctgacgtggc cacctgcatg aacaatcttg ccgggctctg cgatgccagg ggtaattacg
    29581 aagaggccga gaggctcttc cgaaaagctt tcgagatcag gagcaggtcg ctgggcaaag
    29641 ggcaccccga cgtggccgta actctcaaca acattgccgc actttacgag aaccagggtt
    29701 ggttcaccga ggcaaggcct ttgtacaggc gagccctgtc catcctccgg aaagctcttg
    29761 gttccgacca ccccgacgtg gccacctgca taaacaacct cgctggtttt taccggaccc
    29821 aggggaagta tgccaaggca gagaagctct acaagcgatc ccacgacatc cgcaagaagt
    29881 ccctgggagc aaatcacccc tatgtggccg caagcctgac caacatcgcg gaactgtact
    29941 ttgcccaggg caagcactca caggctgaaa gcctcttcaa aaggtcgatc gccatacttg
    30001 aaagagccct tggtcccgat caccctgatg tggctacggg catcaacaac ctggcagggc
    30061 tttacgacac cctcgggaag catgaaatgg ctctccccct gtgccgaaga gctcttgcca
    30121 taaggaccag gtcgttgggg cgctggcacc cccacgtggc ggtaagtctc aacaacctgg
    30181 ccggcctcta tgaatccagg ggcatgttac aacaggcaga tccactttac gggcgctccc
    30241 tgcagatact ggaaaagacc ctcggcccgg cccatccgga tgtggcggtg tgcctcgaca
    30301 atatggccga ccttatgcgg agaatgggcc gcgacgagga agcctcccga atggagcaga
    30361 atgccaggaa gatcaggggg gaagaggagt agttcgagtt caagatcctc acagatcgac
    30421 aattccggaa gcgggaaagg gaggatccgg gacgttaggt tgggaaaatc ccggcctaca
    30481 gaaaaaacct tgatccacct gttccggatc ggcgatccgg ccagatggat cagagatcgt
    30541 cggagatgaa agtggtatac gaactggaat acgacgaaga gaaaaagatc attacagggc
    30601 gaataacagg agagcttaac agagctgtcg cagcagcaat ggcatcacga cttgctgaac
    30661 tgagtcttcg tcatggatgc ttccggttgc tcaacgacct cagggaagcg gatctgatcc
    30721 cgtcggtact ggacatatac atgatcccgc gagaggtcga aaaggccggg atcctgaaga
    30781 ccttcaaacg agccctgttg gtaagaccgc cactggatga tttcaggttc cttgagacag
    30841 tctccttcaa cgagggacac cggttgagag tattcaggga ccctgaagaa gccctccagt
    30901 ggctgttgga atagatcagg ggaagttgaa ttgcaatgct attgtcgcag cgatatacta
    30961 tcactatgaa ataataacca ggcatctcat tcgatccagt gaaagggggc gtggctgtga
    31021 gcggtgtcta taaggtcatc gaactcgtgg gatgcagtga gacctcctgg gaggatgctg
    31081 cgtgctgtgc cgtcgagacc gcctcgaaga gcctcaggga tctccgtgta gccgaggtgg
    31141 taatgcagga cgtcaccatc aaggacaaca agatcgacca gtacagggtc aagctgaagg
    31201 tgtccttcaa gtaccactgt gaggaagatt agcggaagca tcaagatcac aggggggagg
    31261 cattgccgcc ccccctttct gatttccggg aacgggtcac tccatgttcc tggcctccag
    31321 gtccaaaagc catgatttga gctccaggcc tccgccgaag ccgaccagtt tcccgttact
    31381 tccgatgacc ctgtgacagg ggatgaaaat ggcgatgggg ttcttggcgt tggccattcc
    31441 tgcagccctg gaccctccgg gacttccggc agcgatggcc agttccctgt aggtggtcgt
    31501 cctgccgtag gggaccttca acagttccct ccagaccctt ttcatgaatt cagttccttc
    31561 gggcctcagg ggcagcgaga accgttgcaa cttccccccg agatagaggt caagctggcc
    31621 gaaggcttca gccaaaagag gggttccctc tccatcgggt cccttgatcc ttctcgcctt
    31681 gttgggcagg aaaagccttg tgatggaacc ctcatcttcc tcgataccca ctggccctat
    31741 aactgtttcc cttacgctga gcatcagcct tcctccctgg gatcgaaact tgaaatagca
    31801 agcggtcagc gtttacctct cctctttttc ttcaagccat tcgaatatcg cctcagccac
    31861 ggatactctc ttgtcgggaa agtcgtggga gcaggagagg gatatcaccg tcttgtcgcc
    31921 tccggccctt gccagtgtct ccgccaccgg gatctggtgt atcttaggcc aggcacagcg
    31981 atcctcagcg gccatcacga ggagcatcct tctttccgag taggcggagg gggcgccgag
    32041 aagatcccat ctgtcgtggt tgtcgaggat ttcctccatg agagactcag gcgtcgctcc
    32101 gctgaggggt gccatccctc cccggaagag ctccagagtc tccctgaagg cctccttgtc
    32161 ttcatttctg atcctcctgg cctgtttccc gagatcgtat ggcgccaggg gagcgaagcc
    32221 cttgaagtcc agcccgtccc ttgctgcgag gagagtcaga aagccgccaa cactatgtcc
    32281 ggcaaggtag atgttgtccc tgtcgatcct gtgctccctt gcgaaggccg cgtcggccat
    32341 gatgctcctg acggcctgga ggtcactgta gttctggctg aaggagtagc ttcccctgct
    32401 tccccatgat ccggagtagt ggaagaccag cacgttgtaa ccacctctcc gtatcatgtg
    32461 ggcaatgtcg aggttcttct cgttgccggg gaagccgtga agaagcagca gagttttgtg
    32521 tgggccctcg ccgtcggcca ggtagataag tccaaggagc cgttcttgta tgaagaggct
    32581 gtgcatgtcg ggggggtatg tctcatcgat gcaaaggggg tctcggatta tatccttctc
    32641 catctctatc atctcctcta tgtgtcatca atgtcctttt cgttcagtag agatcggtgc
    32701 tcaggtattt gagccccgaa tcgcatacca aaaataccac tgtctttccc ttctctggcc
    32761 cctccagtag cctacgggcc gccgcggcgt gtgcacctga tgagaagccc ccgaagatcc
    32821 cttcaaaacg cgccagagcc cgggctccct cgatggcctc gttgtcgttc atggatacga
    32881 agccgtccac cagggatcgg tccagcaggg gcagctccct ggagtagccg catccctgta
    32941 tccggtgcct gccgcttcct cccgggttcc cgaaaaatgc ggcctcagcc ggttcgccga
    33001 ggtagcatct gattcggggg tcgtgctcct tcagtacgct cgagatcccc gtgaaggagc
    33061 cccccgatcc tgccatatca acgaaggcgt ccaccctgcc ttccgtgtcg cgccatatct
    33121 cctcaccgga gagccgttca cctatcatgt tgcccctcat ttggaactgg tcggcccgga
    33181 aggcgccgcg tcgctccact atgcgtcggg cctcttggtc caccagttca aggtcttctc
    33241 ccgagacctg ccccgggatc gaccccggtg cctgtgggac cagtaccacc tcagcgccca
    33301 gggcacgcat catcagcgct ctttccatgg agttgccctc ggacatgaca gccacgaacc
    33361 tgtacccctt gatcccgcag gccagggcaa ggcctgttcc tgtgttgccg ctggtgagct
    33421 caactaccgt gtcgcctgga gatatccacc ccttttcctc agcctccagg atcatatgga
    33481 ggcccacccg atcctttttg ctgaagccgg ggttaaggtg ttccagtttg gcaaggatcg
    33541 ttccggaaac cccccagtgc tccaccagcc ggctgcagtt tactatcttc gtgttgccta
    33601 ttgcctccag tacgttgtcg agcactttcg tcatggaaga tcctcctgta ttttccgagt
    33661 tcaagtttta aaagactcaa aaagcaatac tggttccgca aggacgttgt gaagatcctt
    33721 tgacaggcca gacgggctgt caggccgaga tcaactcgac gatcttttga atatggatct
    33781 gcgtggtgtc cagtaagggc aattcaaggt cgccttccct gatcaacagg ggcagttccg
    33841 tacaaccaag gacaagggca tcagcccttt tatcggagat catccgtctg gcaatttcga
    33901 gaactgcctt tttctcctcg gggattacta ttccctcctg caggtggggg aagataatgt
    33961 tgtggatcgt cttctggtct ttcccggtcg ggacaaaagc ttccatcccg tatcttgcga
    34021 aggcctctgt gtacaggccg ctgctcattg tgaaggcggt cccgaaaatt acgaccctct
    34081 tgcatttttt tgacagtgca taatggcatg tctcttctat gatactcact aggggaaggg
    34141 gtgacctctt tttgacttcg tcaaaaacaa tatgggcggt gttggcagcc atggcagcga
    34201 gttccgcacc ggcggccaca agccttgtgg tggattctac aaggatgtcc gtgaaaggct
    34261 tccagtcctt tttcgaagcc aggttgtaca tctcggtaag gttaaggctg tctatgacca
    34321 tcaccgggta atcgtccccc cctgttcttt tccggtaacc atctataatc cccttgtagt
    34381 aatccagtgt cgattcgggg ccgatacccc ctaccaggcc gagcttcttc attatcttca
    34441 cctcctgaag atactcctct ttatttttgg ggagcgagaa taaaagcacc aatggttaca
    34501 atacgaaaat agactcacct ggatattccc gcaagtagtc gtcttgtcgt ccctggtcaa
    34561 aaagggcgag aaagaaggaa agaaggaaag gaggaaacaa gtttggtgat cacatttgag
    34621 gaattccaaa aggttgatat gagggtcggg aaggttgtcg aaattgagga tttccccagg
    34681 gcgaagaatc cttcctacag ggtcaggatc gacttcggtg aggagatcgg cataaagagc
    34741 tccagccttc aggcaaaaaa ggactacacc atggaggaga tgctgggccg ttacgtggtc
    34801 tgcgtggtca acttcccgcc caggaacata gcgggattca tgtcggaggt gttggtcctg
    34861 ggcgtccccg gtcaggacga aagcctggta ttactggagc cagaaagggc cccagtgccc
    34921 ctcggcgcga gggtcttcta ggacaaaatc caaggggtgg acaagaacaa gatgacccaa
    34981 ttgatcgttc gatgggctgc aagagttacg ggtgtaggat gcctgctctt atttcttgtg
    35041 ttcttcatcg gtgaaggaga tataagagat atcgtctacc tccaaccccg ggaggtggtt
    35101 ttgatcctgt tcgtaccggt acttttctct gcaggggtcc tggtagcctg gtggagagag
    35161 ccgcaaggcg ggatccttat actggcgagc gttctttggt tcaacgttac agatatagtc
    35221 tcggaaagga agttcaccgg cgagatcgag ttcgccttcc tgattattcc gggtttgctg
    35281 ttcctgctct cgtggtacct ggatggcaga agacgcgaaa ctcatgggcc tggtattcct
    35341 cccggcaacg gaaaaccccg atcatagtat tcaaaaaaag tacattatca aagagaccga
    35401 ttttttgaac aaaagtattc ttgcatcagg atatctcctt tgggtaaaga tgcctgcttt
    35461 cccggtatta agagagagta cgcttatctc aggatcggcc ggtcccgggt ggtctctatt
    35521 gccaggtcat ggcgaagtgt gccatgactg cagagcctat ccatagaaca ctctcgtcga
    35581 ggtcgaactt cgggtggtgg tgagggaaga cctctccttc tcctggcatc gaagggagga
    35641 agaaaaagca tcccggtacc ttttccagaa agaaggccat atcctctccg cccatggtgg
    35701 gctcggcgat ctctgtgaca gcttcctctc ccacgatact tgcagccgcc tctcttagct
    35761 tgtcggtgat ctcgctgtcg ttgttcagag gcggcgcacc agtgatcagc tgcacttcgg
    35821 ccctgccgcg catgctctgg gcgataccct tacagacggt cttcagtcgt tccgagagca
    35881 tggctcttgt tcctgcatcc aggcttctga tagttccgct tatggtacaa ctgggcccta
    35941 tgacgttggg tgcagaacct ccgttgatgc tgcccacagt gatgaccgcc gagttcaggg
    36001 ggctggtctc ccggctgact atggtctgaa tggctatata ggcctgacag gccatggcga
    36061 caggatccac agtcaggtgc ggggtggccc cgtgcccacc tttcccctca aaggtgacgg
    36121 taaaccagtc cgtggccgcc atgagaacgc cgtatcggta gcctaccatc cccgtggagg
    36181 gcccttccca gagattgccc gtgtgaagac cgataatggc atccatggac gggttctcca
    36241 aaacaccgtc acttatcatc gccggggctc cctccgagtt ctcctccgaa ggctggaaga
    36301 tcagcttcac cgatccagga aggtccttac ggttttcagc aagcatcttt gccgttccaa
    36361 gaaggatcgc aacgtgggcg tcgtggccgc aggcatgcat ccgaccgttg accgaggcaa
    36421 aggacaggcc cgtctcctct ttaacaggga gtgcgtcaat gtcggctctc attcccagga
    36481 ccttccccgg cctttccccc ttgataaggg ccgtcacacc gtgcttccca acaccttcga
    36541 ggatctcctc gatccccatc tccctgagcc gctctacgat aaaggcttcc gtcatgggag
    36601 tttcaaaacc agtctccggg ttcctgtgga tgtgacgcct ccattcaata atcttgccgt
    36661 taagttcttt cgccctttcc tttatgttcg tcatttatga ccctccttct attccctttc
    36721 ggagaaccat ctgttccaga tctggtcctt tctgtcagga agtccaccgc aaatcccgca
    36781 accagcttca cccccgtggt cgggcaagcg tttgagtaaa taatatcctt gatggctttt
    36841 tatgtcccgt ctttgatcca gatcttgata ggaggcttag aaaatatttt gttgaataac
    36901 acagccttaa agaaaagaag agaatagaaa agggccccgg tatgacgggg gccctacggt
    36961 tcaggatcct caattacaca tatcaactag tacccgtgcc gtttctcgtt cttctccctg
    37021 gcgaacttgc gggcgttctt cattccgccc atgccagcga acattgctgt tatcttctcc
    37081 gtctctatct gccttgagtt caggtcctcg tacctcgact ccttctcaag cttcataaag
    37141 ttggcaagcc tatcctcggg aaggagtccc tccctgacgg cctgcagaac agcgcagccc
    37201 ggttcgcttg tatgggtgca gtcatggaac ctgcaccttg acgccagttc gtcgatgtcg
    37261 ctgaatgctt tcgataggtc ggccctctct atccccaact ccctcatacc gggagtgtcg
    37321 atcaccactc cgccgttcgg gatgacgaaa agttcccgcc tggtggtggt gtgcctcccc
    37381 ttcccatcgt tccgggtatc tccggtcctg attatctcac tgccgagaag cctgttgata
    37441 agggtcgatt taccaacccc tgaggagccg atgaatgcta tagtccttcc cctgcccaga
    37501 tactggtaca ggggggcata gccgtcatcg gtcaggctcg aagttacgat cacctccacc
    37561 cctggggcta cagaagcaag tttcgacagt tttaccgtta agttatcgca aaggtcctct
    37621 ttggtaagca ccatcacggg tatggccccg ctttcccagg caatcccgag ataacgttca
    37681 gcccggcgaa ggttaaagtc gttgtccaga gacatgcaga tgaagaccgt gtctatgttc
    37741 gctgccacca cctggctatc cctgtttctc cctgcagctt ttctgataaa tgcgctcttc
    37801 ctgggcagga ccttgtgtat cactgcattg ccgctggtgc tgtcgcatcg gtccagcatg
    37861 acgaagtcgc ccactgcagg gaattcacaa ggccccccgg tcttgaaaag gaatcttccc
    37921 gagaccgcag cccgaacctc gccatcttcg gtgacgaccc tgtaaaggtc cctttgctgg
    37981 gagacaatcc gtccgatgtg cagtcccctg tataatctgg atccggccat gagttcctgg
    38041 ctgagcccga tatttcccaa atccactttt ctcaaagtga tcacctccga aaatctgttc
    38101 tcggacagtt ttcgggaggc gtatacattc agatcttcct gtatgcacct ccccggatgg
    38161 tcacaatatc caccttgaga gtaccgagca taaggcacta acccctttcc tgatctcctt
    38221 tgttttgccg ccttgcaacg ggatcatgtg aaaccgcgtg gaccggtcaa ggattatttg
    38281 cggcaccggt cctggaggca ataatacatc accttatggt ccttgggaat ttgggcagat
    38341 caatgccaaa agccggaaga tcaaaacctt ccatgaactt ctttcaagca tccacttgca
    38401 acaatcagac tctcctctgc gaaaaggtcg gtagacgatc catacagctt tcattgcaca
    38461 ctattcgcct tgttaaccaa atcaccttgc aagcattgac agaggtgttt aaagagtggt
    38521 atattccggg acattataat atcgcacaca ggataaaatt aatgtaaata taataaaaaa
    38581 ggatgtttac tgttccgctg cgcgaggaac agggccatta tgagaaggga gggcaggagc
    38641 atataagcgt agcaacaggg aacgggtagc caactctggc tgatcgagat caatccctta
    38701 aggagggtcc acaagatggg tttgacacgt aatgaagaat atcagaaatg cctggaattg
    38761 cttgatgaaa aggaagtggt cggattcctc tcagagctta tcaaggtcga tagcgtgaat
    38821 cctcctggta atgagaagaa cgcagctttg ctggctcaaa gaaagttaga ggaatatggt
    38881 attccatccg aaatcatgga cgtagacagt gaagagccta acagggccaa tctatacgcc
    38941 agaatgggcg atccgtcaca aggcccggct cttctttaca gtggacactt cgatgttgtt
    39001 ccggccggag aaggatgggt gcatgatcct tttggagcag agatcgaggg tgatctcatg
    39061 tatggccgag gaacaagcga tatgaaagcg ggagacgccg ccatgatcat ggccatgtgt
    39121 attttaaaga aagcagacgt gcccctgagt gggacactgg cctttcttgg aacagccgga
    39181 gaagagacgg gtttgatagg cgcaagggat ttcgcaaaaa aacatggatc tgaaaggatc
    39241 gatgcacttg ctatcagtga agcgagcgtc ggggatatct acatcgctca gaaaggagct
    39301 ttttggctga agtttgtttc caaagggaaa agggctcacg gcggtttgcc agagaaaggc
    39361 gttaatgcct tgaagaacat gatacggttc accgaaaggc ttcaggaaga attcaaattc
    39421 aaggtgacca aaagcccttt gcttactcct cccacgttga atcttactgg aatgcatgca
    39481 ggagaactga ccaatattat tcccgacctg tgcgaggcgg ttgtagatat caggaccatt
    39541 ccggggaacc ctcattctga aattcttgca cagatcgagg gcatcctttc agaacttaaa
    39601 aaggaagacg agacgattga tatctcgatc caagtggagt tggatctgac ttcgataagc
    39661 acagatgtcg accatccttt cgtgaagaag gccgttgaat cctatgttgg cgtatttaac
    39721 aaaaaacccg aaattaaagg ggtaacttac ggcacggacg cggttccctt cagagagatc
    39781 gatcctgatc ttccgcttat tatttacggt cctggtgatt catcaagaaa tcataagctt
    39841 gatgagtatg ttgaggtatc gagcgttatt gactcgacca aattttacat cgctcttgct
    39901 ttggaatatc tgaaatagta atttttgcac ttgatcggga aaaggtgttg aaaggaaaca
    39961 cgattggttt aacctttctt taaaaaagta cgggtttccc cccatcagga tatccaaggg
    40021 agtgaaacga ttgaggctct tagtagcgtt tgtaaattgg gtcaacaggc taaacgattg
    40081 ggtgggcagg atcgttggct tccttgtcta tcctatcatg tttgttctgg tcttcgaggt
    40141 tgtgatgcgt tatgccttca acaggcctac catatgggct cacgagacct cctgcatgct
    40201 ttatggggct cattttcttt taggtggagc ttacgccctt agtaaggatg cctttgtcaa
    40261 tgtggaggtt ttttaccagt ctttccgacc cagggtaagg gccatagttg atctcctgac
    40321 ctggacgatg ttttacgcct tcgtcgggac gatgctctgg aagagcatcc cctgggcttt
    40381 agccagctta agggtcggag agtactccga aagcgtctgg gggcctcccc tctggccgat
    40441 caagcttacc ttgccggtgg ctgccggact tatgcttttg cagggaatga cgaagaccgc
    40501 caaagatctt tacctggcct tgacggggag agagcttgta ccagcggtcg aagaaaaagc
    40561 tgttgctgag aactgaggtg atcccatgga cataggcatt atctccattc ttctatttgg
    40621 cagcatgttc tttctcctgg ccatggggct tccagtcgct tttgtgcttg gaggcctggc
    40681 aacggttttt acagctgttt tttgggggcc ggaatccctt ttcataattg ttgccaggac
    40741 ctttgcgatg atgcagacca caacccttgt tgccgctccc ttgttcgttc tcatggcgac
    40801 ggtcctggaa cgttctggag ttgcagagga cctctttgag gttatgtacc ggtggatcgg
    40861 atccatcaag ggggggctgg cagttggaac tgttctggct tgcaccctta tcgccgcaat
    40921 gtcagggatc gcatccacag gtgtggtaat gatgggtgta atggcccttc cggcaatgct
    40981 caaacgtggt tacgacaaga gaatggctac agggtgcatc cttgcgggcg gtgtcctggg
    41041 tccattgatc ccacccagta taggattggt cctttatggg accattgcca aggtctccat
    41101 cggcgagctc ttcgccggag gtatgggagc gggtttgctc tgttcgtcac tgataattct
    41161 ttacatattg ctgaggtgcc atttccaacc tgatatgggt ccggcaattc cttacgaaga
    41221 gagatctgac tggggcgcca agttcgcttc tctgaaagga gtgatcctcc ctcttcttct
    41281 cataacctct gttctgggtt cgatctattc cggaatggca acccccaccg aagccgctgc
    41341 tgtgggcgcc ctgggagctt ttgtctgtag tgctctccat cggaggctgg attggaaact
    41401 gatagccgat gttgcgagga cgactataag ggttcagggt ttcattatgt ggatcctgtt
    41461 ctctgcgcaa gcctttgctg ccgtgtacat ggggctgggt gcagcgaccc ttgtgacaag
    41521 gcttgttgaa acgtacgaga taggttggtg gacgatgctg atctccatcc aggttgtctg
    41581 gttcctcctt ggctgtgtca ttgatgcctg gagcatcctg atgatcacct tgcctatcct
    41641 gatgcccctg atccctgttt acggttttga ccctgtctgg ctgggcgtac tctatgcggt
    41701 caacacacag accggttacc ttacaccgcc tttcggagcc atgctgttca tgataaaagg
    41761 catagctccc aagagtataa ccatgaagga tatctataat tccataactc cctttgttat
    41821 cacccagctg gtttgcctgg cattgtgcat agtgttccct tcagtggtta cctggctacc
    41881 caggcttctc ttcaaataaa ggagaggtgc cgttttatca ggcagttggt tagcgcaaaa
    41941 ggtcccgaac agggcaggag agaggtgatc caatctggcc gaaggagaga tcagaggttt
    42001 ccttgatgag ttttctttga agggggcgtt ttttgtgaag gttcgaaatt tattcttaac
    42061 tggtatagca cttgcgttac tggggtttgt cactgtgggc agttcttccc ccgcaacggc
    42121 ggccgacgag gtatttcatt ggaagtggca ggcccaccag acagccgggt ccctgccttg
    42181 tgagactatc atccctcgtt ttataaaaag agtggatgag atgtccggcg gtaggttgaa
    42241 gatctccttg cactacgccg gtgaactggt ggaattcatg gaagtgcttc ctgctcttca
    42301 ggctaatatg atccagatcg cgaataccgg tgccctgttc tggaggggct cagtcccgat
    42361 gggctggctc acggctggta atctccctcc tttcataaca cgttctgatg ctgagttcaa
    42421 cgaactctat cactacagag gattagacgc acttttgaag gaggcctggg gagagcatgg
    42481 cgttcacttt ctcgggagcc acaacgttgg ggatacttac ttctggagca agaaacctat
    42541 caactctctg gatgaactaa agggattcaa ggtaaggttc ttcggagcca tgtcggacac
    42601 catgcagcat ttcggggcct ctccggtcat gctgccgcac ccggaggtct acatggccat
    42661 ctctaccggt actcttgatg gagcgggaac gtgctggtgg cagtatcgcg acctgaagca
    42721 ctatgaggtt tgcccctatt ttataggccc accctggcag gtcccgcagg caatggagct
    42781 tctggtatcc cagaaggcgt gggaagcgtt gcctgccgac ctccaggcaa tagttgagac
    42841 ggcagctatt gttctctcca aggagtttgc tgatctgacc aggttggaag agaaggaaat
    42901 gttccagaaa tccttcgaag agtgggggac cacctacatt gagtggggtg aagaagacgt
    42961 tgacaggata accaaggagt tctccctgcc ttacctggac aagatcgctg ccgaggaggg
    43021 ctctaaggat ccgagggtgg tcaagggagt agagatcatc aaacagttca tgcaagacta
    43081 cggctatatt gattgatcta aaaccttaag gtatccaagg tggccccctt gccgtttgac
    43141 ttggtaaggg ggccaccttt tttatgacca ggaactgata ttgtccttta agcttatatg
    43201 attgttttgg tggcccactt caactatcac gaaaacatta tcttgttcgg atcccaaatc
    43261 cggtccgtgg ctggcttcgt tgaggcattg acgggcaaca ttgtgcattc aacagataca
    43321 aaagatctgt tttcaatcga tcctggaaag ggttcagaag agacggatga tcgaatcggg
    43381 actgcttctt gccttcagct tcacaaaggc acggcagggc caatagagtg cagaaagccc
    43441 ggtagcccag caggcgtacc cgccccaata acctggaggg gccgggaaca gtttcccgac
    43501 cgccaggaag aagaaaaggt gcaggatgta aaaaaagaac ggacatttgc caaagacctg
    43561 cagtggattg tcctttctgg cccagaaatg tccggcccgg gagaacgatg aaatgataag
    43621 gaggttcacg cctgagaaga gaagcatgta ggccaggctc ggcgggtact tgtttaatgc
    43681 aaggaaggcc atgatcccgc tgcccttgac ggggtgtgta ttgccgaacc cgcctgttgt
    43741 acgcaaaagt acgaaagccg cgatcagagc agagcccgcc agggggagga ctttccctgt
    43801 cccaggacga tctgcctgga tgcgacgagc aaggatcatg ccaaagaggg tgaacacgaa
    43861 ccaggggagt acggggtaaa gaacaaagat ccccagtgtc ggcgtcctta tccccgatgg
    43921 aacaaaggag aacatgaggg gaaggctgac tccgctcata ggttccggag accccaggcg
    43981 ggaagtgatg atccactggg tcgtgagagc aagggtcaac cctatgcctg cgagagcacc
    44041 gtcaggagcc ttgaggagaa aggagccaag agccatggaa cctgccaggg agaagagaac
    44101 gccgaactcg agagagacga cggggctgtc gggccgtgac gggaaggcct gttgccacac
    44161 gaagttctca agggtaagca tgatcactac aagtacgagt gcccggatcc agtagtggcg
    44221 aatgatcctg gactcttccc aaccgacgga gcgcctgctc gtcgcgaaga agaccatccc
    44281 tgcccccatt atgagcgcga acccgggcgc gcaggtgtga gtgataaagc gcgtgaagaa
    44341 cgcctggagc gatccgtaat cgggtacaca catggcccag aactcgaaga accccttgcg
    44401 acagactagt tccatggtgt ggtccagggc catcaacatc attatcaacc ctcgtgcggc
    44461 gtccagttcc ggaagcctcc gatccgtcaa ggtcgtcgac ttgataaggg gttcctctgt
    44521 tctgtttcca tgttccatgg cctctcctgt tattcgaaat tagtgtggtt tcaggtatcc
    44581 actatataaa agatcttgat ctcctttttg tcaaaaagaa gagaggaaaa tgtgtttccc
    44641 agagattttt ttgaaaaaca aaaaaggcag ggaagatcct tccctgcctt tggagaactg
    44701 gactgtaacg tgcacaattt tctacgaatg caaaagatgg gatattcaag tgtcgagatc
    44761 cttgaagttc ttcacttcaa gaatatcttc tcaagttcag atgcaggcag ctgatacttt
    44821 gcagagatct tacggtcctc gccggagatg cgggtggaga tacactccgg ggcccttgtg
    44881 cacccctttt cgccactctg gtagccctta cagacgcgca ggacgtcggg tgatatctcg
    44941 tcaatgagaa cgatcctgcc gtcaccgtcc ttgagacggc cgagctcgat cttgccgtca
    45001 aggacgtgca gaccgtgagc ggcgaattca tcgtagatca caagggctat cttcctgaca
    45061 aagtccttga catgggaaac ctcttcctcg gtcatgaccc cggcacctgt caatgatccg
    45121 aaggtgataa gcctgtcgat cttacccttt gtccaggcct cgacaacctg ggggaggtca
    45181 gcacagggcc tgatgaaggg gtatcgacgc cagaaacttc ccatggcgct gttgcgccag
    45241 gtccactcca ggttcagtag tggggccgaa cctgggaatt ccggcccctg ctctgacatg
    45301 cttatcggtg tggcaggctc cactatcatg acgttgtcgg ccacggtgtc gatgtaatgg
    45361 gttgggatac cctgtcttgc cagaagcctg aaaaagtagg tcgcgaattt gcaggctatg
    45421 gcccccttgc cggggatctc gcccaccacg ttgtcgtatc ctggatcgaa tacgggtttg
    45481 ccgtccttgt cgatataacc tgtgcccctg tcggtgaaga cgaaggcgta cttccccttg
    45541 tatttcccat cggggatgag gtagatgtcc ttggatttgc ctgaggatac aaaattgtcg
    45601 ttgacgagaa tcttgtccag atccatcgga tggcctccct ggttcaataa tgtggttttc
    45661 ccggagtttc tctgtcagtc atcttatcag cctgaaggac aattgaaaat ccggcaccga
    45721 tggatcaaaa gtcctcccgg tctggatcct cctctttccc gttgacgggt gtgcgaatat
    45781 agaaatcctg cccctcccat gatgcttttc tgatctttcc tctccggagc agatcgttga
    45841 tcaggcccca ttcagcacct gaccttgtga tcagatcttc cacggcatct cttctcatag
    45901 ggtgcacggc ggtaatgtcc aggatatcgg cttcggggtc ccccgtatag gcgaaggcat
    45961 tcccttcgta gcctgtgagg agttcggtgc gttcaatgtt caccgaaaaa aggctaaaag
    46021 ctctcaacag ggacctggcc gtagggtgtt cgacccattt ctccatggga ggtctggtgg
    46081 gtatcgacag atatgcacag tggggcttca atttcccaat atgctcagcg gtcgtacgga
    46141 ggctttcttc ggtatcgttg aggttgcgaa cgagcattgt ctcggtaagt agtttcccct
    46201 tatagttcct ggcgaacaca agcgacccct ccaggatggc atccagttgc agggaggggt
    46261 gagggcggtc aatgtgcagc cagacttcgg ggatagcaga gtcaaccttg agggagacca
    46321 ggtcaaaccc ggcgaggtct tcgcggactt catcctttcc catcagcgag gcgttggtaa
    46381 tgatcgccgt cggaaaaccg aattgtttta cgatccggcg ggcctcagac aggttctcgt
    46441 caagggtcgg ttctccgtcg gggacgaagg tcacgtaatc aatgctttcc ccacaagact
    46501 ggaccttttc cattcgttct tccagtgcct tgcctatctc ccggggcttg aaaaaggagc
    46561 tcctgttgga agtgaacacg gtggtgcggc ctatctggca atagacacag gaataactgc
    46621 aggttttggg aggaatggag ttaattccta tgctccttcc aagtcgcctc gacggtaccg
    46681 gaccaaaggt cagcattaca gcatcatccc ctttacctga gtctaaactc gggaatgatg
    46741 cggaaaaggg ccagaagtag agggaaaaaa gtataaactg gattcttcag cctgggctgt
    46801 tgaagaaaag gtttttcaaa aaaacgtgat gacagttctt gccggcacca cgaccaatac
    46861 ttgcacatcg ctttccgttt tctcccaggg gtttcattct atgaatttgt gatcctggat
    46921 ccggcaagga tgcagaggat ctcgtacatg accgttgctg ccacaagggc cgtgctcccc
    46981 gacgggtcaa agggtggtgc aacctcgaca acgtcagccc ccaccaggtt cagccccctg
    47041 aaccctcgga tcagtccctg ggcttccagg gaggtaagtc cacctatttc aggggtgccg
    47101 gtccctggag tgaacgcggg gtcgaggccg tccacgtcga aagtgatata gacaggcccg
    47161 ttgcccacga ttcttcttgc ctcttctacg gtcctttcga cgcctatcct ggcgaactcc
    47221 tccatgaata ctatcctgat gccgttcctt tcaccgaact cccagcattc gggggaattg
    47281 gccgcccccc ttatacctat ctggatcatc cgttcaggat cgataaggtt ttcttcgcag
    47341 gcccggcgga aaggggtccc atggctgtat ttcgagttca tctcctcgtc gcagcagtcg
    47401 gtgtgggcat cgatgtggat cagccccagg gggcgatcca ttgctatggc tcgaagaatg
    47461 ggaagggtta tcgaatggtc cccgccggcg ctgagaggcc agaccccggc gttgttcaga
    47521 cgttcgtaaa aggatgtaat gtccctgtgg cttgcctcaa catcgaatat cctgctgaag
    47581 ggcacgtcac ccacatctcc cacccggcat agttcataag gattcaccct tgtgacgtgg
    47641 tggatggaac gcatcatgct cgacatgtcc ctgatctggc ggggtccctg gcgcgttccc
    47701 gacctcgctt ccacagctcc gtcatagggt acgccgacca gcgcaatatc aatctccacc
    47761 gggtcttcaa tgaaaggagc cctcataaaa gtgggtatcc cgcaataccg gggagcgtgc
    47821 atgggattat ccccgccttg cctgcagtag aagtcgtttc cgggtttcaa gaatatcccc
    47881 tccagaaaac aagaatgttt aaagatctga gttacggata acggatacgg ctattttaac
    47941 ctcttgccag cctgggattc gcacactctt tcccattaac ccttccaata gcatcaaaat
    48001 taggagaggt gtaaaatagc gctgagtcag agtgatagca tcaaatagag aagaggtctt
    48061 agcaagatga ggttgaaagt gttgttgata actgcagctg ccatcctgct aatggtactc
    48121 agtgtcgctc ctccttctga tgccgctttc tataacggcc aggatatcga cgggatagtg
    48181 ttcgatggag cgatctacct ctttgacttt gactcaggcg aacccgtaaa ggctgtcttt
    48241 tcaggctacc aggtgacgat atactggcag aacggagaga tcagcaagta tctgatcgat
    48301 ccgctggaga tagaaatccc ggagtatatc gaacttttca ccgatcccga ggaacctcaa
    48361 cccctggtcg atcccattct cgagatattt ctttagagcc ggggcaattg tgcgacccgc
    48421 atatcaaacc gtgagggggt catcatcatg aaaaagatca acatcgggaa caatccctat
    48481 cctttgccga tgccggtggt catagtagga tccagcattg aggggcgtcc gaattttatg
    48541 gctgtagcct gggttaccag ggtgaacgcc agacctgccc tgatgagcgt cgccattggc
    48601 cgccacgcta cggcatctgc gatcagggat acaggggagt tcagcatcaa tatcccatcg
    48661 gtggaactgg taaaggagac agatcttact ggtatggtca gcgggaacga tcttgacaag
    48721 tcggtccttt tcgatatctt ccacggatcc
//
LOCUS       DAOTIQ010000006        42612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_938_length_42612_cov_7.261790, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000006 DAOTIQ010000000
VERSION     DAOTIQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 42612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42612
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_42612_cov_7.261790"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..885
                     /locus_tag="P5541_01815"
     CDS             169..885
                     /locus_tag="P5541_01815"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRV70741.1"
                     /translation="MKKGNLLYEGKAKKLFETDDPSILLVEYKDSLTAFNAQKKSSLP
                     GKGTLNNRISANLLAHVASEGVPNHFISLIDDTRQTVRKVRIIPLEVVIRNITTGSLC
                     KRLGIEEGIELVTPLLEFYLKDDALGDPIVTVDHIRAFGWATDPEIEKLREISRKVNS
                     ILKKRFLEAGIVLVDFKLEFGRTDEGEVILADEISPDTCRLWDFSSGEKMDKDRFRKD
                     LGNVLEAYAEVWRRLSGEVK"
     gene            885..1142
                     /gene="purS"
                     /locus_tag="P5541_01820"
     CDS             885..1142
                     /gene="purS"
                     /locus_tag="P5541_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRV70742.1"
                     /translation="MKFLAKILVYPKEGVLDTQGKAVARSLKRIGFESLKDARIGKFI
                     QVWIDTENSGEALSVAEAMCSELLVNDLIEDRAIEIEECEQ"
     gene            1139..1846
                     /gene="purQ"
                     /locus_tag="P5541_01825"
     CDS             1139..1846
                     /gene="purQ"
                     /locus_tag="P5541_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRV70743.1"
                     /translation="MKTAVIVFPGSNCDNDVVHAVSMVFGPVVSKVWHEDESLPEGTG
                     LVVLPGGFSYGDYLRCGAMAAKSPIIAAVKEHARKGLPILGICNGFQVLTETGLLTGA
                     LLANRSLSFICDFCHIRVERNDTPFTSRYATGQVLRIPIAHYEGLFYLPQTELEALES
                     EGRVVFRYCSPEGDTDERFSPNGSLNNIAGVINTEGNVLGMMPHPERASEDLLGSTDG
                     ADLFRSVSDWLEGGIQA"
     gene            1843..4002
                     /gene="purL"
                     /locus_tag="P5541_01830"
     CDS             1843..4002
                     /gene="purL"
                     /locus_tag="P5541_01830"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRV70744.1"
                     /translation="MSVNYLDVGLKEQEYLAIMDILGREPNMQELRILGVMWSEHCSY
                     KSTRPLLSMFPNSGGAVVQGPGENAGIVDIGGGWGAAFKVESHNHPSAVSPYQGAATG
                     VGGIIRDILAMGARPVASLDGLCLGDESLQQSRSLADGIVKGIAGYGNAVGVPTVGGK
                     TLYDGCYNGNPLVNAMCLGVVRLDSIVSSKTARPGHLAILVGSRTGRDGIAGAAFASS
                     GLADDTRASRPQVQIGDPFVEKLLIECCLEIHSKGLMVAMQDMGAAGITSSSSEIAAK
                     SGTGIVINSELVPLRENDMEPWEIALSESQERMLLVAEQENLPEISKIAAKWELECAA
                     IGKVTDDGQFVISDKGKIVVSLPAGMVGGSCPVISWTSREPSGRKATSQKGTTFSLSP
                     HMIEKHLVTLLSNPSLSDKSGIFHQYDYMVQTGTVVPPGSAASIIRVRENGKLLGLAM
                     EVDPWKCEIDPFTGSAEVFLKALRSLWVAGAEHLGMTNCLNFPSPEDPGNFWVISRCV
                     EGLAAVARDLVCPVVSGNVSLYNESGDAKILPSPLVGVTGTFRDIAKPLSESFSCENE
                     TVFLAGFSKGTLNGSHFARAMAVEQNHREFPYLPEKEKIFMHSAIETVRKGLATSGRT
                     VAGGGLLVSLAKMCVTSGIGISIDEELPLDDLAVFLFGETGPRAIYTVTENLEKDFAS
                     IWSGIPLIKLGQTGGKTFTIKGLLEIPVNEIARAWRR"
     gene            4008..5384
                     /gene="purF"
                     /locus_tag="P5541_01835"
     CDS             4008..5384
                     /gene="purF"
                     /locus_tag="P5541_01835"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRV70745.1"
                     /translation="MCGVFGAFCKNGDSVLEDVYLGLYALQHRGQESAGIAWNDKDGG
                     KVLSLRGMGLVHIALDQQQLSTIKARSAIGHVRYSTSGASDLSNAQPISANYSMGSLA
                     IAHNGNITNAAGIRNMLESRGAIFQSTTDTETIIHLMAHQSHKPPLDALVDALRKLRG
                     AYSLTVLLGNRLVAARDPWGFRPLVIGQRGDVIYVASESCALDIVGAKLVRDVEPGEI
                     VVIDKTGIKSLRIPLKASRGYSCAFEYVYFARPDSIIDGRSVYDVRKALGCRLAKRSP
                     CPGADIVTGMPDSGTIAAMGFAKCSGQNYEKAIVRNRYIGRTFIQPTQRVRDIGVRIK
                     LNPMKALIGGKELAVIDDSIVRGTTVQRVISMIRQDGAEKVHIRIASPPVRFPCYYGI
                     DTPTCEELAAARLDIETLRKEIGADSLDYIGTEDLVSAIGLPPSNICTACFTGEYLDG
                     GNNDGMDL"
     gene            5368..6360
                     /gene="purM"
                     /locus_tag="P5541_01840"
     CDS             5368..6360
                     /gene="purM"
                     /locus_tag="P5541_01840"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRV70746.1"
                     /translation="MEWTYEKAGVSLQRSDSWIEILREKSKRATNPNVRGGIGGFAGL
                     YDIGGGKILAACTDGVGTKLEVARMTGIFRGLGQDLVAMNVNDLVTCGASPLFFLDYI
                     ACGYLDPEVFGPIMDGIVEACASSGCALLGGETAEMPDVYSDGAFDLAGFAVGAVDGG
                     SLLDGSSIGRGDVLVGLASSGIHSNGFSLVRKCLLEGNDPLPHDLVIPGGERSLGEIL
                     MTPTRLYVKQAASVCRAGLTGGMAHITGGGLEENIRRILPQGVGLSIDFTSWERPAIF
                     ELIKSRGVSEIEMRRVFNLGIGFVFAVPEKNRVRIMDLLRDDFGEDPTVIGEIS"
     gene            6360..6959
                     /gene="purN"
                     /locus_tag="P5541_01845"
     CDS             6360..6959
                     /gene="purN"
                     /locus_tag="P5541_01845"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRV70747.1"
                     /translation="MTTNMAILISGRGTNMLAIADSCITGLLDARISFVASDEPSAKG
                     LEEASRLGIDTRILPYRESGKEKSEEMLQGLLEESGANLIILAGFMRILSPRFVSLWR
                     DRIINIHPSLLPSFPGTGSIRKAWEHGVKVTGVTVHMVDEIVDNGRILAQEPVRIMPG
                     MTLEELEDNIHRTEHILYSRTLRDLLAGKCFARDRKEGV"
     gene            6961..8487
                     /gene="purH"
                     /locus_tag="P5541_01850"
     CDS             6961..8487
                     /gene="purH"
                     /locus_tag="P5541_01850"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRV70748.1"
                     /translation="MPKRALVSVFDKKGISELAKGLESMGWEIVSSSGTATHLRNSGV
                     SVKEVEDFTGYPHMLGGRVKTLHPSVFGGILARRHFEPDLTDVEKFGIPLIDMVVCNL
                     YPFEETFRRGADLDDLLENIDIGGVTLIRAAAKNFRHVIPVTDPEDYPRILEEIGSEG
                     NVTLTFRESLALKAFMKTATYDSIIHEGLSRTCGSEQEKNPLRSVIPMARTMELRYGE
                     NPHQSASLYNNPLDDSPWTLLSGKPLSYNNILDMDCAMRGMALFQSDCACIIIKHTTP
                     CGLAIDETPEKAYEKALECDSVSAFGGIMAFTRTVDADTARKIAGQFAEVIVAPAFEK
                     DASSFLASEKPNLRTVVWKGTRNSALAIHGTWGGILVQEDSLPPIPRQDAGEWIGKPR
                     PDLWDDIIVAWKAAALGKSNSISLVRDGATVGLGRGFTSRVDAVTWACGAAGERSVGS
                     VMGSDAFFPFPDSIEAAAKAGVAAIIQPGGSVRDEEVAQAAVRLGLSMFTSGRRTFRH
                     "
     gene            8502..9773
                     /gene="purD"
                     /locus_tag="P5541_01855"
     CDS             8502..9773
                     /gene="purD"
                     /locus_tag="P5541_01855"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRV70749.1"
                     /translation="MKILVLGGGGREHAIVWALSRSSLAGEIHCFPGNAGISELATVH
                     VMDNFDTTSIMALTERILPDLVIVGPEAPLVAGVSDSLRDRGFPVFGPGAHGARLEGS
                     KAFAKSFMERHGIPTAPFDICENISEAERALEKRTPPFIVKADGLAAGKGVFILDSLV
                     EAREICSGLLEKRILGEAGRKIVIEDHLPGKELTILAATDGKEWRILPSSQDHKRAFD
                     GDRGPNTGGMGAFSPVAFAGAGFLEKIEGSIIEPTVRGLASDSIPYRGILYFGLMIDE
                     AGKARVLEYNVRMGDPETQVVLPSFGGDFAEMALASAKGDLSCVTVPPPSCSSVGVVI
                     ASEGYPGEYRKGFYISGLEKISKRDGVKVFHSGTSKDSKGRTVTSGGRVLTVVGTGDD
                     VFSAREKAYEALDDIEFEGAFFRRDIAINKE"
     gene            9780..10313
                     /gene="purE"
                     /locus_tag="P5541_01860"
     CDS             9780..10313
                     /gene="purE"
                     /locus_tag="P5541_01860"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRV70750.1"
                     /translation="MNKTLVGVIMGSRSDLGIARKAAKTLESLGIDFEVTVASAHRTP
                     EDAASYARNAGSRGIKVIIAIAGLSAALPGVIAAHTPLPVIGVPVNAGSLGGIDALLS
                     VAQMPPGVPVAAMGIDSAKNAALFAARIIALSNSDLQLKMLDETSKAGESVTASRSDL
                     EGLPPAPESAFINLEEK"
     gene            10310..11260
                     /locus_tag="P5541_01865"
     CDS             10310..11260
                     /locus_tag="P5541_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163304.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRV70751.1"
                     /translation="MTISGDRYSNWSSWLRERWGTRVYKIPLDTGAGCPNRDGIERGG
                     CIFCDSLGGGGGAFLRGESLEEQILRGFRRLEKKPGDPMAIIYLQSYSSTNVSADRFA
                     ALIEKTLKISHSLGKVAGLAVGARPDQVPDEILDILEEAGIRNSIDVWLELGIQTVDP
                     SGLEWLRRGHDLAASEDAAARSSGRRLFLCGHLISGIPHERPDQLAGSAIWMANRGFD
                     AVKFHPLHVLRGTALEKEFVCGNFSPQTAEEYVSEVITALRVLPKKMLIQRLCADASP
                     EKLRAPLWINDKSRVIAMIRDRLEAYGAFQGDNFTASSES"
     gene            complement(11238..11453)
                     /locus_tag="P5541_01870"
     CDS             complement(11238..11453)
                     /locus_tag="P5541_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70752.1"
                     /translation="MSPEKGEQNNEKQEKGRSPLTTLLFITVTLALAVKMAIRVPVLR
                     DFFSGYGKFIFLPVLMVLGYLTTQRKQ"
     gene            complement(11640..12152)
                     /gene="tpx"
                     /locus_tag="P5541_01875"
     CDS             complement(11640..12152)
                     /gene="tpx"
                     /locus_tag="P5541_01875"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRV70753.1"
                     /translation="MERNNSVTMKGNPVTLSGPGPKVGEKVPGFSVLDQDLKPVSLDD
                     FAGRIKVISVTPSLDTPVCDLQAHWFNEEASSLPSDVEVINISMDLPFAIRRFCVAGG
                     IDSVRVLSDHRDASFGKGWGILISELRLLARAVFVVDRDNILRYVEIVPEVTSPPDYD
                     SAMEALKSLI"
     gene            complement(12176..12676)
                     /locus_tag="P5541_01880"
     CDS             complement(12176..12676)
                     /locus_tag="P5541_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRV70754.1"
                     /translation="MSNEKTLKNLEAAFSGESQANRKYLAFAKKADAEGFHQVAMLFR
                     AAAEAETIHAHTHLATMGGIGSTEENLKAAVEGETYEFTSMYPSFIEDAAAEGQKRAE
                     RSFRLANEAEKVHAELFVKAAKEPSAGKEFDYYLCTVCGHIAEGSAPSECPLCGAAEK
                     AYRVVK"
     gene            complement(12758..13789)
                     /locus_tag="P5541_01885"
     CDS             complement(12758..13789)
                     /locus_tag="P5541_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRV70755.1"
                     /translation="MKALFIGFGNVGRTIAKILSTEKDLFPGMSRLAGLMPIGIITRS
                     RGSVVDPCGIDFSLALENFGREGSFSEGIPGFTRISGEEASSVLDYDVLVELSTLSIE
                     NEGEPATTHIRNALAGGRHAVSANKGPVSFSYRELSELAIKAGKKFLFETTVMDGAPV
                     LNLCRSCLEGCTIGSVDGIFNSTTNYILTRMEEGADFDDAVREAQVAGVAEADPRNDI
                     DGWDAAAKVSVLANVVLGGNITPLDVERKGISGVSRREVERASLSGRKLKLVCRAWKE
                     GGKVFGKVGVEEIPRDHPFSLVSGGGAVIRIHTDLMGPVLVTQEKPGLYDTAYGVLND
                     LLSLSVSSP"
     gene            complement(13789..15048)
                     /locus_tag="P5541_01890"
     CDS             complement(13789..15048)
                     /locus_tag="P5541_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HRV70756.1"
                     /translation="MKIDNRYLDIPQRRNEFPSLTREHNGLPIAYLDGPGGTQVPRSV
                     MDAVSCYYSTCNSNTHGFFLTTNESDRIIEDARDSMALFLGASGKKNISFGHNMTTLN
                     FSLSRAIGRTLVPGDEILVTQLDHEANRGPWLALREEGVVVREVRLLPGGILDYEDFS
                     DKINENTRLVAMGFSSNALGTVNNVEMVRELTHKSGSWLLVDAVHYAPHFPLDVNSLG
                     VDFLLCSAYKFYGPHIGVLYSREGLLDSLPVYTLRTQEQHAPYCIETGTLNHAAIAGV
                     GAAVDFVASFGSGSSRREKIVSAMNRIGEYEHLLAVEIFEGLRKIKGVEIIGAGFDEK
                     HRAPTVSFTMEGLDPIDVCRMLDSRAICAWDGHFYAIRPIEVLGLLEKGGVTRVGVSL
                     YNTVEEVERLLEAVSDISSGKLSVAGL"
     gene            complement(15154..16299)
                     /locus_tag="P5541_01895"
     CDS             complement(15154..16299)
                     /locus_tag="P5541_01895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRV70757.1"
                     /translation="MISPFSFELPTRIEFGAGVSDSVADALGELGKKRPLLVTDPGVI
                     SAGILDRVKDNLTKAGMDYLVFSSVEPNPKDINVMEGADVALEFGADSLLAVGGGSPI
                     DCAKGIGIIVTHGGNIRDYEGRNAVRKDTLPMVAIPTTSGTGSEVTFSSVITDSREKF
                     KFSIRHPRIAAKIALCDPGMTSSMPPMLTASTGMDALVHAIEGFTALPSEPVADACAL
                     HAIEIIAANLVRAVRQGDDIEARAAMLMGSVLAGISFSHSDVASVHCIAEALGGMYDA
                     PHGLCNAVALATVMEYNRPWCTERYARIASAMGCSFNTAQEGAEMAVEKVSELTRDTG
                     LPPFSTLGVRREDMETIALNSAKNGSNSSNPRPMGRDEYMELLEMMG"
     gene            complement(16363..17349)
                     /locus_tag="P5541_01900"
     CDS             complement(16363..17349)
                     /locus_tag="P5541_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRV70758.1"
                     /translation="MQKKRVLVTFPFDKEGDIAVAEAIDSKAVITRLPDRGDPERGTS
                     LRIADAVISFMMHNELGRDDFHLLKDGCLLQSLQAGVDALPFDDIPETVTICANTGAW
                     ADPMAEHILGMILSLGKYLRKCHERLEKGQFDRSIVSTWFRGKTCGFVGFGGISKAVA
                     RLVRPMGMKVIAVNTSGKTDEDVDFIGDMTSFPKVLKNSDVLVLAVPLTKKTVGLIGS
                     RELSLMKHDAIFINPARGAIIDQKALYEHMKANPDFRVGIDAWWVEPHTHGEFRLDHP
                     FFDLDNLLGSPHNTNLVDGIFPIAARLGADNVRAFLDGMPPRGVVDRRDYEF"
     gene            complement(17383..18567)
                     /locus_tag="P5541_01905"
     CDS             complement(17383..18567)
                     /locus_tag="P5541_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV70759.1"
                     /translation="MTERFPGIDAIHTGDRVTEGNTRPKVPPIYASSVFSFKELEDLD
                     PVFDGDKPGFVYARMGHPNASLLEETISALEGTESALVFSSGMAAITTAFLSVLKPGD
                     HVIADRVLYGGTFSFIDVFLRDWGIEVDLADTLDPDSVKRAIKSNTKVVYLETISNPM
                     MGVADIPALAKISSSAGALLFVDNTFATPILCRPAAMGADVVIHSLTKYLNGHSDVTG
                     GAVAADEGIAGKALRLRSMLGGSMSPFDAWLVQRGVRTLHLRMEAHSTNAIHLARALE
                     RHPRVLRVEYPGLERHSSYGTARKMLENGFGGMLSFEIEGGERAASLFVKNLRMVELV
                     PSLASISTTISHPAKTSHRSMNEAQRADAGITGGLIRLSVGIEPVEPVWQDISEALDS
                     LN"
     regulatory      18752..18869
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            18955..19782
                     /locus_tag="P5541_01910"
     CDS             18955..19782
                     /locus_tag="P5541_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV70760.1"
                     /translation="MNRILKVLAISLLVTIVALSAAFAENPVLRMATTTSTDNTGLLD
                     VLAPEFLKDTGIELQWVSVGTGKALELGKNRDVDVLLVHAPDVEVKFVEDGFGVNRRE
                     VMYNDFVIIGPAKDPAKIRGKMVNEALKAVSGGKFSFASRGDKSGTHVMETVLWKRAG
                     IEQPEKESWYIQTGQGMLNTITIAAERDAYTLTDRGTFIKYEDNHKGDPPLVIIVEGD
                     EQLFNQYSVIAVNPVGADYIRYDLALKFSDWIRSEKIQKMIGDFTLLGKPLFVPNAK"
     gene            19880..20575
                     /locus_tag="P5541_01915"
     CDS             19880..20575
                     /locus_tag="P5541_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70761.1"
                     /translation="MGYLGEGFLTAFRILFSGQEETFSAIITTLKVSTFSILITLAVG
                     VPLGFLLGYYSFPGKNACRMVVDTLLAIPTVVVGLLVYAFISRSGPLGSMGLLFTLKG
                     IAVGQFILGLPVVVSLTASSTEQIENSVRMTLMTLGASPGQLAMSSLWESRYSVIAAA
                     MTAYSRIISEVGVSMMIGGNIKWRTRTITTAIALETGKGQFAEGIALGIVLLFIAFAV
                     SAAVSLSRRRASS"
     gene            20572..21591
                     /locus_tag="P5541_01920"
     CDS             20572..21591
                     /locus_tag="P5541_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV70762.1"
                     /translation="MTHLYELQGITREYGNKKVLDVPELRIEKGRIIGITGPNGSGKS
                     TLMRILAFLEKPSSGDIFFKGKRVRDIDESLRRKATLLLQESRLLKRSVFDNVAYGLK
                     ARGKTEGLSSRVIDSLSIVGLDPSKFASRSWFELSGGESQRVALASRLILDPEVLLLD
                     EPTASIDAGSAAIIKSAILETRERLGTTVVTVSHDLDWAYGISDEVISMFSGRMHSLG
                     PENIIHGPWERAEEGTLLKKLDDGQVITASGFPGSSKTAVIDPEDIIISIDTPENISA
                     RNAIEGTVTRMNLENGSGRILMTIAAGGQVFYARITTLSLQKLEIIPGSAVSIIFKAT
                     AVKWI"
     gene            21660..22655
                     /locus_tag="P5541_01925"
     CDS             21660..22655
                     /locus_tag="P5541_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70763.1"
                     /translation="MTRILSRDHIADIIRMPDVMEAVRTALTERPDRQAPPFQSLISR
                     EERGRVYIVKSSSGFSMIPGTAGVSVTVSGQETSSSGKNIYNSSVILLFDLESGELKA
                     ILDGREITLLRAAASCAIGARLLANKDEKNMLLVGAGGLATAMLKGSVESLPGLKQVS
                     VTSRDNRRTESFINSQSPRYPHLRFVDLSFFDLEDHIGENHYLVTASSSGSPLIKKEW
                     VSPGTHITAAGANSPGRQELDPGIITSSSVFADDADQASRIGECRTAVETGLISPKNI
                     TAIGEVIEGKAAGRTESRQVTVYDMTGTSLEETLVAELALRRSTYRNTGYITGII"
     gene            complement(22662..23144)
                     /locus_tag="P5541_01930"
     CDS             complement(22662..23144)
                     /locus_tag="P5541_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70764.1"
                     /translation="MILFFFSISAAAITFTLFYSWCVQKPVNLVSSFFQGEARPEETS
                     LGEFEKLPPAFRPMIWYPLKGVLFLGETYIQAGWGAYCVLRAYRVISEAGTGTGWNYH
                     TVAFIACIGALGYIARREPRKDILTVIQSCVAMGSYIVFCLTPKALGTYYPWLVNYFK
                     "
     gene            complement(23245..23415)
                     /locus_tag="P5541_01935"
     CDS             complement(23245..23415)
                     /locus_tag="P5541_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70765.1"
                     /translation="MDIEMKVIRRKSADPGASGRFRSSYPDSLFDGNQAQINYRWREK
                     LLVTDLVRTLSD"
     gene            complement(23474..24649)
                     /locus_tag="P5541_01940"
     CDS             complement(23474..24649)
                     /locus_tag="P5541_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRV70766.1"
                     /translation="MENRHRIEEILELSGKKAKILISGRVFHPAGGGQPGDSGTLEGE
                     GFRADVVDCHKGDGGDILTIRIREGTLETGSIVSAFVDEERNLRLSRMHTGEHIFSRA
                     LEDLFEGLRVEKVWIGEKESTIYIKFTGEIGWDEIFHAEEKANSVIGEDLPITRDERS
                     KCDFEEFEGVRIKWDRLEEEKIAVISIRGYDSIACSGSHVSSTGLVGGIIATGFRGSP
                     PDWEIRFTVDRQEFLEKQSRMVRVLLRDVGCEEEKLPSVVQKLQEERRELSKKIDRVK
                     SFVEIPWERHRCKGFDVFVFSAENFPVEVATGSMKKKMANEPDAFVLFLSRADENNEV
                     SFVLAAGQNLGSDLRAIPRENPFLSARGGGSADWIQGVAASASISEWKDAISRKVDK"
     gene            24720..25007
                     /locus_tag="P5541_01945"
     CDS             24720..25007
                     /locus_tag="P5541_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRV70767.1"
                     /translation="MENRVCKRVLISGAVQGVGFRWRARQLALELGISGWIRNRPDGK
                     VEAVFQGPDIMVREIIDWARKGPKGAVVTELCQFDYECDPVPFDFVIKRNA"
     gene            complement(25024..26265)
                     /locus_tag="P5541_01950"
     CDS             complement(25024..26265)
                     /locus_tag="P5541_01950"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRV70768.1"
                     /translation="MEYKGKVVLAYSGGLDTSVAVKWLQDQGYDVVTFTADVGQNIDL
                     DQVKMKALAVGAVNAYVMDLRQEFVETMVWPALKSNAMYQGKYPLNSALSRPLIAKYL
                     AWVAEIEGAVAVAHGCTGKGQDQVRIEVCTNALNPDLKVIAPVRDWHFSRDAEIEYAR
                     VNNIPVPVTVESPYSIDGNLWGRSIECGPLEDPWTEPPSDAFTMTVDPWEAPDNHEYL
                     EISFLEGIPVALNGKEMDGVTLISELNAIAGGHGVGRIDMIEDRLVGFKSREVYECPA
                     TVVLLQAHQALESMTLSRDVIAAKADLSLKYAELTYFGYWYSPLRKGLDAFFDSIQEN
                     VTGTVRVRLYKGASVVVGLKAEKSLYVDSMATYSEGDAFDHEAAAGFIKIWGLPVKLW
                     RTVHPEAENIKPELKVVGEGK"
     gene            complement(26741..27520)
                     /gene="surE"
                     /locus_tag="P5541_01955"
     CDS             complement(26741..27520)
                     /gene="surE"
                     /locus_tag="P5541_01955"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRV70769.1"
                     /translation="MKILVSNDDGVFSPGIITLSRVLKEHGYSPSVVAPDRERSSVGH
                     SITLTRPLRLWEIEGGGYPEGMKVYACEGTPSDCVVLGLEAVAPESEMVISGINRGPN
                     LADDLTYSGTVSAAMEGHILGRPSIAVSLNSAQSDKVENYESAAESVATILEGFKNFP
                     FPEGILLNVNVPNLPAGKIRGFRITKKGIRIYRDKVNPLKDPKGRSYYWISGQPDDVM
                     DDGSDVKAVSEGYVSITPIHMDMTHYPSIEKLRRAGFEKDS"
     gene            complement(27540..28145)
                     /locus_tag="P5541_01960"
     CDS             complement(27540..28145)
                     /locus_tag="P5541_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV70770.1"
                     /translation="MSHFPALGDLLLSLPAVLWAITFHEFCHGYAAFRLGDPTAQRMG
                     RLTLNPLAHLDPIGALMLLVFRFGWAKPVPVEPRYFKHPRRDLIIVSLAGSAGNFLTA
                     VFSGLVVRLVPGPFMSNPALGRFILLMILINLGLGVFNLIPIPPLDGSKLLYVLLPPK
                     YMGWFFWLERYGFFVLMILLVLGVVQAIMSPIIRTLLYLII"
     gene            complement(28152..30797)
                     /locus_tag="P5541_01965"
     CDS             complement(28152..30797)
                     /locus_tag="P5541_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV70771.1"
                     /translation="MKIITTHIGADFDSLASMVAASKLYPGARPCFSGSAGRNVREFL
                     KRNPGRWNILTPSQVEMDKISTLIVVDARSPARIASFAGILERQDLEVHIYDHHAAVQ
                     DDITADFSLIEPVGATTTLLVEMLIDKGIEITPQEATLFAMGIYEDTGALTFGATTER
                     DFMAVIRLRSMGSDMTAIPAHIELSLDSSERRILDRLIESARERYIQGAKVVLSSMSH
                     PDYVEGLSLFVHRLRDYFDADVALAAVRMEKRVYVVARGKEEILDVAKFLSPLGGGGH
                     SQAASVTLTGEDPQEILGRLESRLEKEIQPLVTVEDVMTSPVMVITPDMSVEEAYRLM
                     IRYGHAALPVSVDGRLLGIITRKDLDKARIHGLGSALVEDYMTGDPVTILPDASVAEA
                     HRMMVLRNIGRIPVTSGEEILGIVTRTDILRALFPRSLPVETRSVSPEMPWMENVSGT
                     MSGILDTKVLSLLYKLGDRASSLGFQAYVVGGFVRDLLLARPNEDLDIVVEGDAVAFL
                     ESWVKDGCRVAIHQRFRTGTIVFPDGRKVDVATARREFYEYPVAQPRVSIDSLKHDLY
                     RRDYSVNAMAVSITRGSWGVLVDYFGGRNDTGNRSLRVLHNLSFVEDPTRVIRGIRLE
                     QRLDFTIEDNTLRLLENCIKGGLLSLLSGVRLRSEIELVFREEKALSVVRRLKYLGFW
                     RSLFPGLILDIGAARALRRLGAFRKRLMRDIPDLGEKEWLASLMAVLVDSPDGVETRV
                     LDRLSLSPGEREIVIRGIFELGSAEHRLGGRGEKKPSHIMMFLQEYSPVTAIFWAAAT
                     GRWRVRRRILLYLTRLQKVEPMLSGRDVLEMGYHEGPLVGVILSNLKMARLDGEVESR
                     EDEIEWIKENYSAGSFPALQDTERK"
     gene            complement(30766..31644)
                     /gene="coaE"
                     /locus_tag="P5541_01970"
     CDS             complement(30766..31644)
                     /gene="coaE"
                     /locus_tag="P5541_01970"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRV70772.1"
                     /translation="MLVVGLTGDVGAGKSTVSSIWASAGARVLSADAIVAKLWRTEEL
                     VLAAVDRWGESILDPSGYPDHAAISALVFDNDNEYRWICDTVHPLARREMYRWVKELD
                     GWVVAEIPLLFENGVPEWIDLTVYVEAPEICRISRNAARGWDAREIHRRQKRLMPTET
                     KRDKADFVLLNDGGLQDLSERAMILAKGFIDASSLTGFFVRSQDGEISPCLKDALSGY
                     SEVVGMEPCPDKKAINVWARPGYIEKIRDILGECCPEDVFTMETGDATLSVPKRILLR
                     VAGGETDEDYNYTHRG"
     gene            complement(31604..33580)
                     /locus_tag="P5541_01975"
     CDS             complement(31604..33580)
                     /locus_tag="P5541_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRV70773.1"
                     /translation="MTVFRKTALAGSLNDTQAEAVRECSGPLLVLAGAGSGKTRVLTH
                     KFAWLVSEEGLEPSSILAVTFTNKAAREMQERVCNLLGTGIRGMDIGTFHSYGLRFLI
                     RHSKDLERIANRKISSIFDRADTRSLVKSVMKDLDLDQKRFEPGWVLESISRAKTECD
                     FRSLSPRPLDFPVDRIFPKYQEGLAKQKAADFDDLILLPLHLLLNDGEILEEERERFR
                     WILVDEYQDVNRSQYTLLKLLSGKDPRIMVVGDPDQSIYGWRGADMNMILNFEKDFPK
                     AKVVVLEQNYRSTGNILGAANGIIRNNSKRKPKKLWTSRGGGEKIHVLLGRNEREEAI
                     FVASEIMRLRSQGYGFGDMAILYRINAMSRLYEEALLQNDIPYRIVRGTAFYERREVK
                     DAIAFMRLAVNPMDFAALERVANVPSRGLGKKSLERLMGHIAGKSVPAYEIWKGIASG
                     VSGLASKAAAGAAELATHMMRILTIQDDIVAVINYIMQDIGYRDELSKIDPEGWHDRH
                     ENVMELLSVAATGEGLETMLAEIALVTDQDLDEDDSEKVSLMSLHAAKGLEFPSVFLV
                     GLEEAVFPHYRCMDDPENLEEERRLCYVGMTRAEERLYMTAARSRVLFGNVLRNGFSR
                     FLWEIPDEFKDTEDRGEEGIPHACGRPDWRRWGR"
     gene            complement(33577..34233)
                     /gene="hypB"
                     /locus_tag="P5541_01980"
     CDS             complement(33577..34233)
                     /gene="hypB"
                     /locus_tag="P5541_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRV70774.1"
                     /translation="MPRKISILKSVMAADEKIAMRLRGELEKRGILMVNLIGSPGAGK
                     TTLLEATLKKGGFRSAVIEGDVATSRDAERIALTGTPVIQINTHGGCHLEANLIEKAL
                     LEMDLEGVEVLFIENVGNLVCPAEFDLGEDFKIAVCSVPEGPDKPLKYPHLISMAKAV
                     ILTKVDLAEYLEFDMKLFWNDVISLNPNASRFEVASTSGAGIDEWISFLSACMEAKSK
                     "
     gene            complement(34217..34573)
                     /gene="hypA"
                     /locus_tag="P5541_01985"
     CDS             complement(34217..34573)
                     /gene="hypA"
                     /locus_tag="P5541_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRV70775.1"
                     /translation="MHELSLVKALVDLLEQQARENRWGRITRVTIKIGSMRQVIPDVM
                     SFSFDIAARETLMEGAELEIIELPVELRCRSCCKEWEKDPFLCPECGSSDVEIRQGME
                     MEIESVEVEDQDAPKD"
     gene            complement(34574..36442)
                     /locus_tag="P5541_01990"
     CDS             complement(34574..36442)
                     /locus_tag="P5541_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV70776.1"
                     /translation="MTACSYDCPDACPLIVKNKQSGPLLIGPDPTFPLGFGFICDKGK
                     RWESVRTHPERIKSPLLKNGSRWDEISWDKAWSVWAARTERALSRNGPLSIFFSRGYG
                     SLYFSKNLLPNFFAALGGYSTTVGSLCGAAGGAGLRKSFGTTPVMLPETVMEKSNGVL
                     LWGRNAAVTNRHLLPLLSGIRNRGGGVVSIEVRETETTRISDKWWRIRPGSDGVLALG
                     LCATLYRSGLLPDRWKVGVQNLPAFEKLIGELDLRDVEEATSLKEKDVVEIAEWILKN
                     GPVAVYPGYGIQRYMSGSDTFNSLVALAVMIGGLSEPGCGVVFGKDEMALFPDSLTRQ
                     CAVQRKLPVSSWYSSGGLSPGIDVAVFCGSNPAKQSPGSNVFTETLSKIPYKVCCDLF
                     MTETARMCDLVLPSSLFLEEGPDWRGSWWHQYVFRSLKVCDSPGEVLTDLEIFDGLAR
                     KMGVSSDLEGQRLEMDSILRKDSRLRQVSEGVYYWDEPEHWGSGKYSVRLPEETPKSS
                     SFDGEFRLISVHSNEYINGQTICQENTTGLPIAVVSLDDLGGLGALEGSEGVIRGESG
                     SMRVVIKADSSIPKGICIMKQGIPGVNSLTIPLVSPGYGAPFHENRVSINMVRRCL"
     gene            complement(36580..37122)
                     /gene="raiA"
                     /locus_tag="P5541_01995"
     CDS             complement(36580..37122)
                     /gene="raiA"
                     /locus_tag="P5541_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRV70777.1"
                     /translation="MDIRFVTRNVELQDDLQEYIEKKLLKLEKFFDRIRDTQVEVSFN
                     RGMNVVEITADVNGVIMRGEDYAPDIRKAFDKCLKSIERQIKKHKSFLKDKARMKPVD
                     VSFEIEGFVREISEPEENLEKIVKTKKFPLRPMSPEEATMQMDLMGHSFFVFRSADSG
                     SVNVVYKRKDGGYGLLVPEE"
     gene            37319..38515
                     /locus_tag="P5541_02000"
     CDS             37319..38515
                     /locus_tag="P5541_02000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRV70778.1"
                     /translation="MKFSARASRMEPSATLEVVAKAKNLKNQGKPVISFGAGEPDFDS
                     PPAAREYAIKAINDGQTHYTQSSGLPELKEAVTDYYRKRFGLDYSPGQVVIGAGAKPL
                     LYETLAALIDPGDEVIVFSPAWVSYVEQIRLLDGKELIVDTTETGCVPNIAVTERMIT
                     PATRGILVNTPNNPTGAVYREDVLRDIASLAKKHDLWIIFDEIYERLVYGNTQFRNIL
                     EIAPEVYDRTILVNGVSKAFAMTGWRIGYALAPEWLTPKIASIQGHLTSNPCSIAQWA
                     ALGALTGSEEDTEKMRLAFSERRDLMIDMLRRMPHISFSKPEGAFYVFVDIRKTLGYS
                     FRGRTIVDDGDFCSILLDSEFLAAVPGSAFFAPGFIRLGYANSENDIREGMMRFHRFL
                     DQINKK"
     gene            complement(38564..39121)
                     /gene="frr"
                     /locus_tag="P5541_02005"
     CDS             complement(38564..39121)
                     /gene="frr"
                     /locus_tag="P5541_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRV70779.1"
                     /translation="MPELEIKRMKEKMEKAVEHLRKDLLGVRTGRAHPALVSEIKVDY
                     YGAQTPISQVGAISTPDARQIVITPWDKTAIKAVEKAIQASSLGINPNVDGENIRLVI
                     PELTGERRVELTKLVKKYAEDARIAVRNLRRDSNDTFRKMEKEGNISEDSMHDFLESI
                     QKTTGEFIKSVDEIMAAKEKEILED"
     gene            complement(39127..39849)
                     /gene="pyrH"
                     /locus_tag="P5541_02010"
     CDS             complement(39127..39849)
                     /gene="pyrH"
                     /locus_tag="P5541_02010"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRV70780.1"
                     /translation="MGSYRRVLLKLSGEVLAGDSPFGLDMGAIKKICREIASIAESGL
                     EIAMVVGGGNIIRGAQVTGAELGRAQGDYMGMLATVINSLALQNVLEHLGIPTRVQTA
                     IEMREIAEPFIRRKALRHLEKGRIVIFAAGTGSPFFSTDTAAALRSAEISADCLLKAT
                     KVDGIYDDDPAKNPEAKLLPRITYMEALQKQLKVMDAAAFSLCMENRIPILVANILKE
                     GTLRDILINGKQVGTVVHLEKE"
     gene            complement(39921..40514)
                     /gene="tsf"
                     /locus_tag="P5541_02015"
     CDS             complement(39921..40514)
                     /gene="tsf"
                     /locus_tag="P5541_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRV70781.1"
                     /translation="MSVDVDAVKELRYRSGAGVLDCRKALSECGNDLEKAVDFLREKG
                     LAKAAKKAGREASEGRIFSYIHTNGKIGTLVELDCETDFVARTDEFQELGKEIAMHIA
                     AAGPSYLTSEEVPQNDLDREKEIFRQQALDEGKPEHIVDKIAEGRIAKFYEENCLMEQ
                     KYIRDPEKKIKDLVVEEIAKLGENIVVKRFSRFSIGE"
     gene            complement(40514..41359)
                     /gene="rpsB"
                     /locus_tag="P5541_02020"
     CDS             complement(40514..41359)
                     /gene="rpsB"
                     /locus_tag="P5541_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRV70782.1"
                     /translation="MAVVSMKQLLECGVHFGHQTRRWNPKMKPYIFTERNGVYIVDLQ
                     KTVKGLEKAYDFLREVSKGGGSVLFVGTKKQAQETIREESVRCGQYFITHRWLGGLLT
                     NFPTIKKRVARMVELGSPSFLEGSTKWTKKEIAGFAKEREKLEKYLLGIKDMKDIPDA
                     LVVVDPKREDIAILEARKLGIPIVAIVDTNCDPDLIDFPIPGNDDAIRAIKLIVGLMA
                     SAVIEGHQGTDNVCADSADDTDAEDDAGQQNGVSEEAHEKFGEVETVIVDKELLERKG
                     WKEAQ"
     gene            41572..41892
                     /locus_tag="P5541_02025"
     CDS             41572..41892
                     /locus_tag="P5541_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRV70783.1"
                     /translation="MAKTSLIEQLEKMDPARKAMLNRLKRVEGQLRGIQRMIIEDKPC
                     QDILLQLSAARKAMQNACIEILKSYVKRCLAESRSPDMGELETLIGTLIDIAPVSPSS
                     EVES"
     gene            complement(41916..42395)
                     /locus_tag="P5541_02030"
     CDS             complement(41916..42395)
                     /locus_tag="P5541_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70784.1"
                     /translation="MSLDEKFSGHFLPSVKWIEGDILFLQPLYLVPVEVFTTGMLKRP
                     EYKTVVAVIEAVGGRVESLRKNMPAISEGNPEGRKLPVNLAPEKAVRIAECALVSEGR
                     EGWRALAGSSHVMARDRDMRSCWKIWIRSGESLIDSVTGKEFPAGEFLGMMLPRELD"
     gene            complement(42436..42535)
                     /gene="ffs"
                     /locus_tag="P5541_02035"
     ncRNA           complement(42436..42535)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5541_02035"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT        10378 a        11565 c        10497 g        10172 t
ORIGIN      
        1 cttttaagcc agccgttagc gattctaaca tggaacgtta tttgtgtcac gaattgaaca
       61 acccaaattg atcaagacga acagacaaat aaaagtcacg agttttcgtt gacatttatc
      121 atctaaaggc aaataatgta agcataaaaa tcaatggggg tatgactgtt gaaaaaggga
      181 aatctactat acgaaggcaa ggccaagaaa cttttcgaga ccgatgatcc ttcgatcctg
      241 cttgtcgagt acaaggacag cctgacggct ttcaacgccc agaaaaaatc cagccttcct
      301 ggcaagggaa ctcttaacaa caggataagc gcaaacctgc ttgcccacgt cgcttcagag
      361 ggagtcccta accattttat cagcctgata gacgacaccc gccagaccgt cagaaaggtc
      421 aggataattc ctctcgaggt ggtaataagg aacattacca ccggctctct atgcaaaagg
      481 ctggggatag aggaaggaat cgaactcgtc acaccccttc tggagttcta cctcaaggac
      541 gacgccctgg gagatcccat agtcaccgtg gaccacataa gagctttcgg ctgggcaact
      601 gacccggaga tcgaaaaatt gagggagatc agcaggaaag tcaactctat cctgaaaaaa
      661 agattccttg aagcgggaat tgttctcgtg gatttcaagc tggagttcgg gagaacagac
      721 gagggagaag taattctggc cgatgagatc tcgccagata catgtcgcct ctgggatttt
      781 tcaagcggag aaaaaatgga caaggaccgt ttccgcaagg atcttggaaa tgttctggag
      841 gcttacgccg aggtctggcg aagattgtcg ggggaggtca aataatgaag ttcctcgcaa
      901 agatactggt ttatccaaaa gagggagtcc tggacaccca gggtaaggcc gtggcgaggt
      961 cactgaagag gataggcttc gaatccctta aagacgcaag gataggaaaa tttatacagg
     1021 tatggataga taccgagaac tccggcgaag ccctttctgt cgccgaggcc atgtgctctg
     1081 aacttctggt aaacgatctc atagaggatc gcgcgattga gatcgaagaa tgcgaacaat
     1141 gaagaccgcc gttattgttt ttccgggaag caattgcgat aacgatgtgg tccacgcggt
     1201 ctccatggtg tttggccccg tagtttcaaa ggtttggcac gaggatgaat ccttgccgga
     1261 aggaaccggc ctggtggttc ttccaggagg tttttcctac ggagattatc ttcgctgcgg
     1321 cgcaatggcc gccaagtccc cgataatagc cgccgtaaag gaacatgccc gcaaaggcct
     1381 cccgatcctc ggtatatgca atggtttcca ggtccttacc gaaacggggc ttctgaccgg
     1441 agcccttctt gccaaccgtt cactctcctt tatatgcgac ttctgccata tcagggtcga
     1501 gagaaacgac actcctttca cctcacgtta cgcaacagga caggttctcc ggattcccat
     1561 agcacattac gagggattgt tttacctgcc ccagacagaa ctggaagccc tggaaagtga
     1621 gggcagggtg gttttccgtt attgcagccc tgaaggagat accgatgaac gattctcccc
     1681 caacggatcc ctgaacaata tagccggagt gatcaacaca gaagggaacg ttctcggaat
     1741 gatgccgcac ccggaaagag cctccgaaga tctgctggga tcaaccgacg gggcagatct
     1801 tttcagatcc gtctcagatt ggctcgaagg aggtatccag gcatgagcgt gaactatctc
     1861 gatgtcggtc ttaaagaaca ggaatacctc gctatcatgg acatccttgg acgggaaccc
     1921 aacatgcagg aactgcgcat tctgggcgta atgtggtctg agcattgcag ttacaaatct
     1981 accagaccac ttctttcgat gttccccaat tccggaggcg ccgtcgttca gggcccggga
     2041 gaaaacgccg ggatagtgga tattggaggg ggatggggag ccgctttcaa ggtcgagagc
     2101 cacaaccacc cctctgccgt ttccccttac cagggagccg ctacaggcgt aggaggaata
     2161 atcagggata tcctggccat gggggcaaga ccagtggctt cccttgacgg gctgtgcctg
     2221 ggagatgaat ccctgcagca gtctaggtct cttgcggacg gaattgtaaa agggatcgcc
     2281 ggttacggaa atgccgtagg tgtcccaacg gtaggcggca agactcttta cgatggatgc
     2341 tacaacggca acccactggt aaacgcgatg tgtctcggtg tggtcaggct ggattccata
     2401 gtcagttcaa agacggcgcg accagggcat ctggcgatac tggtgggctc caggaccgga
     2461 agagacggga tcgccggagc cgcctttgcc tcttcgggcc tggccgatga cacaagggcc
     2521 agcaggccac aggtccagat cggggatcct ttcgttgaaa aactccttat agagtgctgt
     2581 ctggagatcc attcgaaggg gcttatggtc gcgatgcagg atatgggcgc cgcgggcatc
     2641 acttcttctt cgagcgagat cgcggcaaaa agcggaacag gaatagtgat caactccgaa
     2701 ctggttcctt tgagggagaa tgatatggag ccatgggaga tagccctgtc cgaatcccag
     2761 gagagaatgc ttctggtcgc ggaacaggaa aatttgccag aaatcagcaa aattgccgca
     2821 aaatgggaac tggagtgcgc ggcgatcggc aaagtcaccg atgatggcca gttcgttatc
     2881 tcggacaagg gcaagatagt cgtaagcctt cccgctggaa tggtcggagg aagctgcccg
     2941 gtcatatcct ggacatccag agaaccctcg gggagaaaag caacttctca gaagggaacc
     3001 actttcagtc tgtccccaca catgatcgaa aagcaccttg ttactcttct ttcaaatcct
     3061 tcactgtccg acaaatcggg gattttccac caatacgatt atatggtcca gaccgggaca
     3121 gtcgttcctc ccggcagtgc ggcatccatc atccgtgtaa gggagaacgg gaaacttctt
     3181 ggactggcca tggaagtgga tccctggaaa tgcgaaatcg atcccttcac cggttcggca
     3241 gaggtcttcc tgaaggccct gcggtctctc tgggtcgccg gagccgaaca tctgggtatg
     3301 accaactgcc ttaactttcc ctcccctgag gacccaggca atttctgggt catctccaga
     3361 tgcgtcgaag gtctagcggc agttgccaga gacctggtgt gtccggtagt atccggaaac
     3421 gtaagccttt acaacgaaag cggcgacgca aagatactcc cctctccgct ggtcggggtc
     3481 accggaacat tccgggatat tgcaaaacct ctcagcgaat ccttttcctg tgagaacgag
     3541 acggtttttt tagccggctt cagcaaggga acgctcaacg gaagccattt cgcaagagca
     3601 atggccgtag aacagaacca cagggagttt ccctacctgc cggagaagga aaaaatcttc
     3661 atgcattctg ccatcgaaac ggtcagaaag ggccttgcga cttccgggag aacagtggcg
     3721 ggcggaggtc ttctggtttc ccttgccaaa atgtgcgtca cttctggcat cggaatatcc
     3781 atcgatgagg aattaccact cgacgatctc gcggttttcc ttttcgggga gacgggcccc
     3841 agggcgattt acactgtaac tgaaaacctg gaaaaagatt ttgcctccat atggtctggt
     3901 attcctctca ttaagttggg acagactggc ggcaagactt ttacgatcaa gggccttctc
     3961 gagatccccg tcaacgagat cgcccgggcc tggaggcgct gagagccatg tgcggtgttt
     4021 ttggagcatt ctgcaagaac ggggactccg tcctcgaaga cgtctacctc ggcctttacg
     4081 cccttcagca cagggggcag gagtctgccg gaatagcctg gaacgataag gatggaggga
     4141 aagtcctctc tttgaggggc atggggctcg ttcatatagc ccttgaccag cagcagctct
     4201 ccaccatcaa ggcgcgttca gccataggac acgtgaggta ttcaacatcg ggagcatcag
     4261 acctttccaa cgctcagcct ataagcgcaa actattctat gggctctttg gctatagctc
     4321 ataacggaaa cattaccaac gcggccggaa taaggaacat gctggagtcc agaggagcca
     4381 ttttccagtc gaccaccgac acggaaacaa taattcacct gatggcgcac cagtcccaca
     4441 aacctcccct tgacgccctt gtggacgcat tgagaaaact ccgcggagcc tattccctga
     4501 cggtcctcct ggggaacagg cttgtcgcgg ccagagaccc ctggggattc cggccccttg
     4561 tgataggaca aagaggagac gtgatctatg tcgcgtcgga aagttgtgcc cttgatatcg
     4621 tcggggctaa acttgtcaga gacgtcgaac cgggagaaat cgtcgttata gacaaaacag
     4681 gaataaaaag tctcaggatc cctctcaagg catcgagagg ctattcctgt gccttcgagt
     4741 acgtatattt cgctcgccct gacagtatca tcgacggaag atccgtctac gacgtaagaa
     4801 aggccctggg ctgcagattg gcaaagagat cgccctgccc gggcgcggat atagttaccg
     4861 gaatgcccga cagcgggact atagcggcta tgggattcgc caaatgctcc ggacagaatt
     4921 atgagaaggc cattgtcagg aaccgttaca tcgggaggac atttatccag ccaacgcaaa
     4981 gagtaaggga cataggtgtc cggataaaac ttaatcccat gaaggcactt ataggaggaa
     5041 aagaacttgc cgttatcgat gactcgatag tcaggggaac cacggttcaa agagtcatct
     5101 caatgatcag acaggacgga gccgaaaaag tccacatcag gatcgcttcg ccaccggtcc
     5161 gcttcccctg ctactacggg atagacacac ccacctgcga ggagctggcg gcggccagac
     5221 tggacatcga aacgctcaga aaagagatcg gagccgactc ccttgactac attggtacgg
     5281 aagacctggt ctcggccatc ggccttccgc catcaaatat ctgcaccgcg tgtttcaccg
     5341 gtgaatacct tgacggaggg aacaacgatg gaatggacct atgaaaaggc aggagtaagc
     5401 ctccagagaa gcgattcctg gatagaaatt ctccgggaaa agtccaaaag agccaccaac
     5461 cccaatgtca ggggtggtat aggcggcttc gcaggtcttt atgacatcgg ggggggtaag
     5521 atactcgctg catgcaccga tggagtcggg accaaacttg aggttgcccg gatgaccgga
     5581 atattcaggg gtcttggaca ggaccttgtt gccatgaacg ttaacgacct ggttacctgc
     5641 ggtgcttctc cacttttctt tctggactac atagcctgtg ggtacctgga ccctgaggtc
     5701 ttcggcccca tcatggacgg aatcgtcgag gcctgcgctt catcgggatg tgccctgctg
     5761 ggaggagaaa cggccgaaat gcccgacgtt tacagcgacg gagctttcga ccttgccggt
     5821 tttgccgtag gagctgttga tggggggtct cttctggatg gaagcagtat agggagaggc
     5881 gatgttcttg tcggtcttgc cagttccggt atccacagca acggtttcag cctggtaaga
     5941 aagtgtcttc ttgagggaaa tgacccgctg ccacacgatc tcgtgatacc gggaggcgaa
     6001 agatcactag gcgaaatcct tatgaccccg accagacttt acgtcaaaca ggctgcttcg
     6061 gtatgcaggg ctggactgac gggaggaatg gctcatataa cgggaggagg ccttgaggaa
     6121 aatatccgca gaatattgcc tcagggtgtt gggctttcga tagatttcac ctcgtgggaa
     6181 agaccggcca tcttcgaact gatcaaatcc aggggtgttt ccgagattga gatgaggcga
     6241 gtcttcaacc tcggaatagg ttttgtcttc gcagtacccg aaaagaacag agtaaggatc
     6301 atggatcttt taagggatga ctttggggaa gatcctacag ttattggaga aattagctga
     6361 tgaccaccaa catggcaatc ctcatatcgg gcaggggaac aaacatgctt gctatcgctg
     6421 atagttgcat cacagggcta ctggacgcgc ggatatcctt cgttgcctct gatgaaccgt
     6481 ctgccaaagg tctggaagaa gcatccagac ttggcattga tacccggatc cttccctaca
     6541 gggaatcggg gaaagaaaag agtgaagaga tgcttcaggg cctccttgag gaatccggag
     6601 ccaacctgat cattcttgcc ggtttcatgc gaattctttc acccaggttt gtgtccctct
     6661 ggagggacag gattattaat atccacccgt ccctcctccc ctcttttccg ggaacaggat
     6721 caataaggaa agcctgggag cacggggtaa aagtcacagg ggtcacggtg catatggtcg
     6781 atgaaattgt ggataatgga aggatactgg cccaggaacc ggtcaggata atgccgggaa
     6841 tgacactgga agaacttgag gacaatatcc acagaaccga gcacattctc tattccagga
     6901 cactgcgtga tcttcttgcg gggaagtgct tcgcaagaga cagaaaggag ggggtctgag
     6961 atgccgaaac gggcacttgt ttccgttttc gacaaaaagg gcatatcgga acttgcgaaa
     7021 ggccttgaga gcatgggttg ggagatagta tcaagttccg gcacagcgac acacctgaga
     7081 aattccggag tgtcggtaaa ggaagttgag gattttaccg gttaccccca catgctgggg
     7141 ggaagggtaa agacccttca tccttctgtt ttcgggggaa ttctggcacg tcgccatttt
     7201 gaacccgatc tgactgatgt agagaagttc ggcataccgc tgatagatat ggtcgtctgc
     7261 aacctttacc cttttgagga gacctttcgc cgtggggctg atcttgatga cctactggaa
     7321 aacatcgata ttggcggggt gaccctgata agagccgcgg caaaaaattt ccggcacgtc
     7381 atacccgtaa ccgatccgga ggattatccc aggattcttg aagagatagg atccgaggga
     7441 aatgtaaccc tcaccttccg tgagtcactg gcacttaagg cctttatgaa aaccgcaacc
     7501 tatgattcga tcattcacga gggcctgtcc aggacctgcg gctccgaaca ggaaaaaaac
     7561 cctctccgtt cggtcatacc catggccagg accatggagc tgagatacgg agaaaacccc
     7621 caccagagcg cctccctcta caacaacccc ctggatgatt caccatggac actcctttcg
     7681 ggaaaacctc tctcctacaa caacatcctg gacatggact gcgcgatgcg gggcatggct
     7741 cttttccagt ctgactgtgc ctgtatcatc atcaaacata caacgccctg cgggctggcc
     7801 attgatgaaa ccccggagaa agcttatgaa aaggccctcg aatgcgactc tgtatcggcc
     7861 ttcggtggca tcatggcctt caccagaacc gtagacgccg acaccgccag aaagatcgcg
     7921 ggccagttcg ccgaagtgat cgtggcacca gccttcgaaa aggacgcttc atccttcctg
     7981 gcatcggaaa aaccaaacct gaggacagta gtatggaaag ggaccaggaa ttcagctttg
     8041 gcgatccacg ggacctgggg ggggatactg gtccaggagg actctttgcc gccgatcccc
     8101 cgacaggatg cgggtgaatg gatcggcaag ccaagaccag acctgtggga tgatattatc
     8161 gtagcctgga aggctgctgc cctgggaaaa agcaattcta tctcgctggt tcgtgatggc
     8221 gcgacggtgg gcctcgggag gggcttcacg agtcgtgtcg acgctgttac ctgggcttgc
     8281 ggagcagcgg gtgaaagatc agtcggttcc gttatgggct ctgatgcgtt cttccctttc
     8341 cctgacagta tcgaggcagc cgcaaaagcg ggcgtagcgg ccataatcca gccggggggt
     8401 tccgtcaggg acgaagaagt ggcacaggcc gcggtaagac tcggcttgag catgttcaca
     8461 agcggacgga gaaccttccg tcactgatgg gggttgacgc catgaagatc ctcgtactgg
     8521 gtggaggggg aagggagcac gccatagtct gggcgctttc aagatcttct cttgctggag
     8581 agattcattg ctttcccgga aacgcgggta tttcggaact cgccactgtt catgtcatgg
     8641 ataattttga cacgaccagc attatggccc ttacggaaag gatccttccc gacctcgtta
     8701 tagtcggtcc tgaagcccct ctggtcgcgg gggtttccga ctcactcagg gacagaggct
     8761 tccctgtctt cggaccaggc gctcacggag ccaggcttga gggaagcaag gcctttgcca
     8821 agagtttcat ggaaaggcac gggataccaa ccgccccttt tgatatttgc gaaaatattt
     8881 ccgaagcgga acgcgccctg gagaaaagga cacctccatt tatcgtcaag gccgacgggc
     8941 tggcggcagg gaagggggtc tttattctgg attcccttgt tgaagccagg gagatatgct
     9001 cgggactgct tgaaaaaagg atccttgggg aagctggacg caaaattgtt atagaagacc
     9061 accttcccgg taaggaactg actatccttg cggcaaccga cggaaaagaa tggagaattc
     9121 ttccttcaag ccaggatcac aaaagagctt ttgacggcga ccgcggccct aacaccgggg
     9181 ggatgggagc cttttcgccg gtggctttcg ctggcgccgg tttcctggaa aagatcgaag
     9241 gatcgatcat agaacctaca gtcaggggcc tggcatcgga ttcgatcccg tacaggggaa
     9301 tcctttactt cgggcttatg atcgacgaag caggcaaagc aagggtactt gaatataacg
     9361 tcaggatggg agaccctgaa acacaggtgg tcctgccttc ctttggagga gatttcgccg
     9421 aaatggctct ggcgtccgcg aagggagacc tgtcctgtgt aaccgtgccc cctccttcct
     9481 gctcctctgt gggcgtcgtt atagcctcgg agggttaccc cggagaatac agaaaaggct
     9541 tttatatttc gggacttgag aagatttcca agagggatgg agtaaaagtt ttccattccg
     9601 ggacatcgaa ggactccaaa ggcaggacgg taacatccgg aggaagggtc cttaccgtcg
     9661 tagggaccgg agatgatgta ttctcggcaa gggaaaaggc ctacgaagcc ctcgatgata
     9721 ttgaatttga aggtgccttt ttccggagag atattgcgat caataaggag tgatctcttt
     9781 tgaacaaaac ccttgtagga gtgatcatgg gatcccgttc agatctcggc attgcacgaa
     9841 aggctgcaaa aaccctggaa tccctcggta ttgatttcga ggttacggtt gcctcggcgc
     9901 accgaacccc cgaagatgcc gcgtcctatg ccaggaacgc tggatccaga ggaataaaag
     9961 tgataatcgc tatagccggg ctttcagccg cgcttccagg tgtgatcgcg gctcacacac
    10021 cgcttccagt aataggagtc cctgttaacg ccggatccct cggcgggatc gacgcccttc
    10081 tttctgtcgc gcaaatgccg ccaggagttc ctgtcgcggc gatgggtata gattccgcaa
    10141 agaacgccgc ccttttcgcc gcacgtatta tcgccctttc gaacagtgac cttcaactca
    10201 aaatgctgga tgaaacatcg aaggctggag agtccgtcac ggcgtcaaga tccgacctcg
    10261 aaggactccc ccccgcaccc gaaagcgctt ttatcaattt ggaggaaaag tgacgatctc
    10321 gggagacaga tactcgaact ggtcctcatg gctcagggaa cgctggggaa ccagggtgta
    10381 caagatcccc cttgatactg gagccggatg ccccaaccgc gacgggatcg aaagaggtgg
    10441 atgcatcttc tgcgattcac tgggtggcgg gggtggtgcc ttcctgagag gtgaaagcct
    10501 ggaagagcag atactgcgag gtttcaggag gctcgagaaa aaacctggtg accccatggc
    10561 gataatctat ctgcagagtt actcttcgac aaacgtctcg gcagacagat ttgctgccct
    10621 gatagaaaaa acgctgaaaa tttcacatag cctgggaaaa gtagccggac tggcggtcgg
    10681 ggctcgtcca gatcaggttc ccgacgagat cctcgacatt ctcgaggaag caggaataag
    10741 aaattccata gatgtatggc tggaactcgg catccagacc gtggatccgt ccggacttga
    10801 gtggctgcgc cgcggacatg accttgccgc ttccgaggat gccgccgcaa ggtcatctgg
    10861 aaggcggttg ttcctgtgcg gtcaccttat ctcgggcatc cctcacgagc gcccggacca
    10921 gcttgcagga tccgctatct ggatggctaa caggggtttt gacgctgtaa agttccaccc
    10981 gctgcatgtt ctccggggaa cagccctgga aaaagaattt gtctgcggga atttctcccc
    11041 gcagacagcg gaggaatatg tctcggaagt cataaccgcc ctgagggtgc tgccaaaaaa
    11101 gatgctcata cagcgtctct gcgccgacgc ttctccggaa aagctacggg ctcccttgtg
    11161 gataaatgac aaaagcagag tgattgccat gatccgcgac cggcttgagg cgtacggcgc
    11221 tttccaggga gataatttta ctgcttcctc tgagtcgtaa gatacccgag gaccattagt
    11281 acagggagaa atataaattt accgtaaccg gaaaaaaagt ccctgagcac aggaacacgg
    11341 atagccatct taacggccag cgccagggtt acagtaataa aaaggagcgt agtaaggggg
    11401 gatctcccct tctcctgttt ttcgttgttc tgttcccctt tttcgggact cattccccaa
    11461 cctccgatca atgattttcc ccgacccgac tcattttctc accagcgttc atcaaggtca
    11521 ttgcctggca ataacttaca agggagccgg gagccggttt accctttcac agtctactat
    11581 acagcatggg gaaaaaataa aaaaaggggc tttcaaaagc ccccattgtt ccggtgatct
    11641 cagatcagag acttcagcgc ctccatggcc gaatcgtaat cgggaggaga agtgacctcg
    11701 ggaactatct ccacgtaacg aagaatatta tccctgtcta caacaaacac agcccttgcg
    11761 agaagtctca gttcacttat cagaattccc cagcccttcc cgaaggaggc atccctgtga
    11821 tcagaaagaa ccctgacaga gtctattcct ccggcaacgc agaatcttct gatcgcgaag
    11881 ggcagatcca tactgatatt gataacctcc acatcggatg gaagcgagga agcctcctcg
    11941 ttaaaccagt gagcctgcaa gtcgcacacg ggagtatcaa gagacggggt cacagatatc
    12001 accttgatcc tcccggcgaa atcatccagc gataccggtt tcagatcctg gtcaaggacg
    12061 gaaaacccgg ggaccttttc tcccaccttc ggcccgggtc cggaaagggt taccgggttt
    12121 cccttcatgg ttacggaatt atttctttcc actgccgagc caccaccctt tcaggttatt
    12181 tcactaccct gtatgccttc tccgccgctc cgcaaagagg acattcagag ggagctgaac
    12241 cttcggcaat atgtccgcag acagtacata aatagtaatc gaactctttc cccgcagatg
    12301 gttctttcgc ggcctttaca aacagttccg catgaacctt ttcggcctcg ttggcaagcc
    12361 tgaaggatct ctcggccctt ttctgcccct ctgctgcggc atcctcaata aatgacggat
    12421 acatgctggt gaattcgtat gtctctccct ctacagccgc cttcaagttt tcttcggtag
    12481 agccgatccc ccccatggtc gcgaggtgag tgtgggcgtg gatggtctcc gcttcggcag
    12541 ctgccctgaa cagcatggca acctgatgga agccttctgc atctgctttt ttcgcaaaag
    12601 ccagatattt cctgtttgcc tgtgattcac cggaaaaggc tgcttcaaga tttttcaatg
    12661 ttttttcgtt tgacattgcg gtatccaccc cttttactgg aatgttttca atttaccatg
    12721 gcccagcccc ctttgcaacc atgtattcac ctccggttca gggagaggag accgaaagag
    12781 aaagcaggtc gttcagaacc ccataggctg tatcataaag acccggcttc tcctgtgtaa
    12841 caaggacagg tcccatgaga tccgtatgta tccttatcac ggccccacct ccggaaacaa
    12901 gagaaaaggg gtgatcccga ggtatctctt ctaccccaac cttgccaaaa acttttcccc
    12961 cctctttcca tgcccgacag acaagtttaa gtttcctgcc agacagggag gctctttcga
    13021 cttcccggcg agagacacct gatatgccct tcctttcgac atccagagga gtaatattgc
    13081 ctccaagcac aacgttagca aggacagaaa ccttggcagc cgcatcccag ccatcaatat
    13141 cattgcgggg atcagcttca gcgacacccg caacctgggc ttcacgaaca gcatcgtcga
    13201 aatccgcgcc ctcttccatc ctcgtcagga tatagttcgt tgtagaattg aaaatcccgt
    13261 caacggatcc tatggtgcaa ccttcaagac aagatctgca aaggttcagg acaggggccc
    13321 cgtccatgac ggtagtctcg aagagaaact ttttccctgc ctttatcgcc aactctgaca
    13381 gttccctata ggaaaaagag acaggtccct tgttggccga gactgcatgg cgtccacctg
    13441 caagggcatt cctaatatgt gtagttgctg gctctccttc gttctcgatg gaaagagtgg
    13501 acaattccac aagaacatcg taatcgagga ccgaacttgc ttcctctccc gaaatacgtg
    13561 taaacccagg aataccttca gaaaaagaac cttccctccc gaaattttca agggccaaag
    13621 agaagtcaat accgcaagga tccacaacag accccctgct tctcgtgatt atcccgatcg
    13681 gcataaggcc agcaagtcgg gacattcccg ggaagagatc tttttcagtc gaaaggatct
    13741 tcgctatggt tcttccgacg ttaccgaaac ctatgaagag agccttcatc aaagccctgc
    13801 cacagacagt tttcccgagg atatgtcgct gacagcttcg agaaggcgct caacttcctc
    13861 gacggtgttg taaagtgata ctccgaccct tgttacccct cccttttcga gaagtcccag
    13921 cacttcgatc gggcggatag cgtaaaaatg tccgtcccac gcgcagatgg cacggctgtc
    13981 cagcatcctg catacatcga ttgggtcaag accctccatt gtaaaagaga ccgtgggggc
    14041 acggtgtttt tcatcaaaac ccgccccgat gatctctact cccttgatct ttctcaaccc
    14101 ctcgaatatt tctacagcaa gaaggtgctc gtattcaccg atcctgttca tggcactgac
    14161 tatcttctcc ctccgggaag acccgctgcc gaaagaggct acgaaatcaa cggcagcccc
    14221 tactcctgca atagcggcat gattaagggt ccccgtttcg atgcagtagg gggcgtgctg
    14281 ttcctgtgtc ctcagggtat acacggggag actgtcgaga aggccctccc tggaataaag
    14341 aactcctata tggggcccgt agaacttgta ggctgagcaa aggagaaaat ccacacccag
    14401 ggagttgaca tccagaggaa agtgaggagc atagtggact gcatcgacca ggagccagga
    14461 gccggatttg tgcgtaagtt ccctcaccat ctccacattg ttcaccgttc caagcgcgtt
    14521 ggaagaaaat cccatcgcaa caagccttgt attctcgttt atcttgtcgc tgaaatcttc
    14581 ataatcaagg atgcctcccg ggagaagccg tacctccctc accacgactc cctcttctcg
    14641 aagcgcaagc cacggtcctc tgttggcttc gtggtccagt tgggtcacaa gtatttcgtc
    14701 tcccggaaca agagtcctgc ctatggctcg gctcagtgaa aaattcagtg tagtcatgtt
    14761 gtgcccaaag gatatgttct tctttccgct ggccccaagg aagagggcca tggaatccct
    14821 ggcgtcctct attatcctgt cagattcatt cgttgtgaga aaaaaaccgt gcgtgttgga
    14881 gttgcaggtg ctgtagtagc aagagaccgc gtccataacc gaccggggga cctgagttcc
    14941 tccgggaccg tcaagatagg caatgggaag accgttgtgt tctctggtaa gtgacgggaa
    15001 ttcatttctc ctctgtggga tatcaagata cctgttgtca attttcatta aaaatactcc
    15061 ttccgtgatt tttcagggca caactcctga tacggtcttt tatttctgga ctctcactgc
    15121 gataagtgat cttcgatctg caaatttttg tctttacccc atcatttcga gcagttccat
    15181 gtactcgtcc ctgcccattg gcctcggatt actggaattg ctgccgtttt tagctgaatt
    15241 gagagctatt gtttccatat cttctcttcg aacacccagg gtactaaatg gcggaagacc
    15301 cgtatcacgg gtcagttccg agaccttttc tacagccatt tctgcccctt cttgcgcagt
    15361 attgaaagag caacccattg ccgaagctat cctcgcgtat ctttctgtgc accagggcct
    15421 gttatattcc atgacggttg ccagggctac agcgttgcaa agaccgtggg gagcatcgta
    15481 cattcctccc agggcctcgg caatacagtg aaccgaagca acatccgagt gagaaaaaga
    15541 gatcccggca agaacactac ccataagcat ggcagccctg gcttcgatat cgtcgccttg
    15601 tcggacagcc cggacaagat tggccgcaat gatctctatc gcatgcaggg cacacgcgtc
    15661 agcaacaggt tccgagggca gggccgtaaa accttctatc gcatgtacca aggcatccat
    15721 tccggtcgac gccgtaagca tgggcggcat ggatgacgtc attcctggat cacaaagggc
    15781 tatcttcgcg gcaattcggg ggtgtcttat gctgaacttg aatttctccc tgctgtcggt
    15841 tattacggaa ctgaaagtta cctcgcttcc ggtgccagat gtagtaggta ttgccaccat
    15901 gggcagagtg tctttacgaa cagcatttct gccttcgtaa tccctgatat ttcctccgtg
    15961 agttacaatt attcctatcc ccttggcaca gtctatcggg ctccctccac cgaccgccag
    16021 aagggagtcg gcaccaaatt caagggccac atccgcccct tccatgacgt tgatgtcctt
    16081 ggggttgggt tctacggagc tgaaaacaag gtagtccatt cctgccttgg tcaggttgtc
    16141 tttgactctg tcaagaatgc ctgccgaaat gacccccggg tcggtaacca acaatggcct
    16201 cttcttcccg agctcaccaa gggcgtccgc tactgaatct gatacgcctg ccccgaattc
    16261 tattctggtt ggaagttcaa aactgaacgg acttatcact tgaccacgcc tcccgaatat
    16321 ttcaggttga ttctgccaga tgccatttgc gggcaccagg gtctaaaact cgtagtctct
    16381 tctgtcaaca acgcctcggg gaggcattcc gtcaagaaaa gccctgacat tatcggctcc
    16441 cagccttgcc gcgatcggga agatcccgtc aaccagattc gtattgtgcg gagaacccag
    16501 taaattatcc agatcaaaga aggggtgatc cagcctgaat tcgccatgag tatggggctc
    16561 gacccaccag gcatctattc ccacccggaa atcggggttg gccttcatgt gttcgtagag
    16621 agccttctgg tcgatgatcg ctccacgcgc ggggttgata aaaatggcat cgtgcttcat
    16681 cagcgagagt tccctgctgc ctataagccc aactgttttt ttggttagcg ggacggccag
    16741 aaccaggaca tcggaattct tcaggacttt ggggaaagat gtcatgtccc ctatgaaatc
    16801 gacatcctca tcggttttcc cactggtgtt taccgcaata actttcatcc ccatcggccg
    16861 gacaagcctt gcaacagcct ttgatattcc gccgaaaccg acaaaaccac acgtttttcc
    16921 ccgaaaccag gtactgacta tgcttctgtc gaactggcct ttttcaagtc tctcgtgaca
    16981 ctttcgcaga tacttcccaa gggagaggat cattcccaga atatgttctg ccatgggatc
    17041 agcccaggct ccggtattgg cacatatcgt caccgtttcg ggaatatcgt caaaaggaag
    17101 ggcatccaca cctgcctgaa gggactgaag aaggcatcca tccttcagga gatgaaagtc
    17161 atcccggcca agttcgttat gcatcatgaa ggatataacc gcgtcggcaa ttctcagaga
    17221 agtacccctt tccggatcac cacggtcggg cagtctcgta atgacagcct tgctgtctat
    17281 cgcctccgct accgcaatgt ctccttcctt gtcgaacggg aaggttacca gcactctctt
    17341 tttctgcatg gagcaactct cccttctttg attactcaag gatcaattca gcgaatcaag
    17401 agcttccgaa atgtcctgcc aaaccggctc caccggctct atacccacgg aaagccttat
    17461 aagtcctccg gtgatgcccg cgtccgctcg ctgcgcctcg ttcattgacc gatgagaggt
    17521 ttttgcggga tgagaaatag tcgtggaaat ggaggccagg cttggaacaa gttcaaccat
    17581 cctgagattt ttgacgaaaa gggaagcggc tctctcaccg ccctcgatct cgaagctaag
    17641 catccctcca aaaccgtttt caagcatctt ccttgccgtg ccgtaactgg aatgcctctc
    17701 cagccccggg tactccaccc tcaggactct ggggtgcctt tcgagagccc tcgcaaggtg
    17761 aatggcattg gttgaatgcg cttccattct caggtgaagt gtcctgaccc cccgctgaac
    17821 aagccatgcg tcaaaagggc tcatgcttcc tcccagcatc gagcgaagtc tgagcgcctt
    17881 tccagcaatc ccttcatcag ctgccactgc cccacccgta acatcgctgt gtccattaag
    17941 atatttcgtg agactgtgga tcaccacgtc ggcacccatc gctgccggcc tgcacaggat
    18001 cggtgtagca aaagtgttgt ccacgaaaag caaggcaccc gcagaggagg aaatttttgc
    18061 cagagcgggg atatcggcaa cacccatcat ggggttggat atagtctcaa ggtaaacgac
    18121 cttagtgtta gactttatcg ctctttttac cgaatcaggg tccaatgtgt ccgcaagatc
    18181 gacctctatc ccccagtctc tcaggaaaac atcgataaag gaaaaggtac ctccataaag
    18241 aaccctgtca gcaattacgt gatcacccgg tttgagaacg ctcaggaaag ctgtcgttat
    18301 cgctgccatc cccgaagaaa atacaagggc agattctgtc ccttcgaggg cagatattgt
    18361 ctcctcgaga agagaggcgt tagggtgccc catccgtgcg tatacgaacc ccggtttatc
    18421 cccatcaaaa acagggtcca ggtcttcaag ttctttgaag gagaatacgg agctggcgta
    18481 aattggagga accttgggtc ttgtatttcc ttccgtgacc ctgtctccag tatgaattgc
    18541 gtcgattccc ggaaaccttt cagtcatctt tcaaccctcc gtcgaaaaga ttggttatta
    18601 ttcgcctgta aagatattat aaagcctgga atggcaattt tcggtagcgg tttaactttg
    18661 cgtcctttcc ggaaatgtct ctatatttcc tgcccggcga tacgacaatt acgggcactt
    18721 tactgtttgc tcctgtttta gtaaactgca ttatgacaaa agcctccgag cccttccgcc
    18781 taaaggagcc cccacggcgg cgggcctgcg ggtgttcccg gaaacaggaa cacctcccgt
    18841 gtttggaaag gagggaacgg acagggttat cgacggccgt ctccctttat caacgggaga
    18901 cggtttattg ttaccgggca caagcccggt attcttctga agggattgat tcagatgaat
    18961 cgtatcctta aggtactggc aatatctctt cttgtaacga tagtagccct ttcagctgct
    19021 ttcgcggaaa acccggtgct tcgaatggcc acaacaacaa gcacagacaa cacaggcctc
    19081 ctcgatgttc tcgcccccga attccttaaa gataccggca ttgagctcca gtgggtttcg
    19141 gtgggtactg gaaaggccct tgagctcgga aagaaccgtg acgtggacgt tcttttggtt
    19201 catgcccccg atgttgaagt aaagttcgta gaggatggtt tcggggtgaa caggcgcgag
    19261 gtcatgtaca acgacttcgt tattatcggc ccggccaagg accccgcaaa gatcaggggg
    19321 aaaatggtaa acgaggcact taaagccgtt tcgggaggaa aattctcttt cgcaagcagg
    19381 ggcgacaaat ctggcacgca cgtgatggag accgttctct ggaaaagagc ggggatcgag
    19441 caaccagaaa aggagagctg gtacatccag accggccagg gaatgctgaa caccataacc
    19501 atagctgcag agagagacgc ttacaccctc acggacaggg gcaccttcat caaatacgag
    19561 gacaaccaca aaggagatcc gcctctggta atcatagtcg agggagacga acaacttttc
    19621 aatcagtata gcgtgatcgc agtaaacccc gtaggagcgg attatattcg atacgacctt
    19681 gctctaaagt tttctgactg gatccgctcg gaaaaaatcc agaaaatgat cggtgatttc
    19741 actctcctgg gcaaacccct cttcgtgccc aacgcaaaat aaactgccac ccctgagagt
    19801 ctcctcgttg cgggatagtt atttccccaa tggggaggct tttttattcc ctcatgtaca
    19861 atattcttgg aggttttgta tgggctatct gggtgagggt tttttgacag ctttccggat
    19921 ccttttttct ggtcaggagg agaccttcag tgccattatc acaacgctga aagtctcaac
    19981 cttttccata ttgataactc ttgccgtggg agttcccctg ggcttcctgc tgggatatta
    20041 ttccttcccg ggcaaaaacg cctgtcgaat ggtcgtcgac actctactgg ctattccgac
    20101 agttgttgtt ggacttcttg tctatgcttt tatttcgaga agcggccctc tgggcagcat
    20161 gggccttctt tttacactca aggggatcgc cgtcgggcaa ttcattctcg ggcttccggt
    20221 agtggtatca ctgacggctt cgtctactga acagatcgaa aactcggtca ggatgaccct
    20281 tatgacactt ggagccagcc ctgggcagct tgcgatgagt tctctgtggg aaagccggta
    20341 ttcggttatc gcagcggcca tgactgccta cagcaggatc atttccgagg ttggcgtttc
    20401 catgatgatc ggcgggaaca tcaaatggcg cacaaggaca ataactaccg cgatagccct
    20461 tgaaacagga aaggggcaat tcgcggaggg tatcgcactg ggtatagtcc ttctgtttat
    20521 tgcattcgcg gtaagcgcgg ctgtctcgtt atccagaagg agggcttcga gttgacgcat
    20581 ctttatgaac tccagggaat aacccgggaa tacggcaaca agaaggtcct tgacgtaccg
    20641 gaactgcgaa ttgagaaggg gcgtataatc gggataacgg gccccaacgg aagcggcaag
    20701 agcacgctaa tgaggatcct tgcctttctg gaaaagccat cttccggaga tattttcttc
    20761 aagggcaaaa gggtacggga tattgacgaa tcattaagga gaaaggccac actgcttctt
    20821 caggaatcca gactcctcaa gagatccgtc ttcgacaatg tcgcctacgg cctgaaggca
    20881 agaggaaaga cagaaggact ttcttcgaga gtcatcgact cgctttcaat tgtgggactg
    20941 gatccatcga agttcgcttc ccgctcctgg ttcgaacttt ccggaggaga atcccagaga
    21001 gtcgctctcg cctcaaggct tatcctcgat cccgaagtgc ttctcctgga cgaacccaca
    21061 gcaagcatcg acgcaggaag cgcagcaatt ataaagagcg ccattctcga gacaagggaa
    21121 aggctcggga caacagtggt aacggtaagt catgacctgg actgggcata cggcatttcg
    21181 gacgaggtca tatcgatgtt ttccggaaga atgcactccc tgggcccgga gaatatcatc
    21241 catggcccct gggaaagagc agaagaggga actttactta agaaactgga cgatggacag
    21301 gttattaccg cctccggttt cccgggttct tcaaaaaccg cggtaattga tccggaagac
    21361 attatcatat ccattgatac tccggaaaac atttccgcaa ggaatgccat agaaggaacg
    21421 gtgaccagga tgaacctgga gaacggatcc ggaaggatcc tcatgacaat cgcagctgga
    21481 gggcaggttt tttacgcaag aataacgacg ttatcacttc agaaactcga gattatacct
    21541 gggtcggcag tatcgatcat tttcaaggcc accgcagtaa aatggatatg atcattccat
    21601 gaaaggagct caaaaccttt gctccggatc atcgacgata aggagggttg gcattgaaca
    21661 tgacaaggat cctgtcaagg gaccacatag ccgatatcat ccgcatgcca gacgtgatgg
    21721 aggccgtgag aacagccctt acagaaagac ccgatagaca ggcacctcca tttcaaagcc
    21781 tcatttcccg ggaagaaagg ggacgtgtct atatcgtaaa atcctcttcg ggattctcga
    21841 tgatccccgg gacagccgga gtctccgtaa cggtttcggg gcaggagaca tcatcttcgg
    21901 ggaaaaatat atataattct tcggttattt tgcttttcga tcttgaatca ggggaactga
    21961 aggcaattct tgatggcagg gagatcaccc ttttgcgagc cgcagcgtca tgcgcaatag
    22021 gagcaaggct tcttgccaac aaggatgaaa aaaacatgct ccttgtagga gcgggcggcc
    22081 tcgcaacagc aatgctaaaa gggtccgtcg aatccctgcc cgggcttaag caggtttcag
    22141 tcacaagccg ggataacagg agaacagaaa gtttcatcaa ttcgcaatcc ccccgctacc
    22201 cccatctgcg tttcgttgat ctttcattct ttgatcttga agaccacatc ggtgaaaacc
    22261 actatcttgt aacagcatct tcctcggggt caccgctaat aaagaaagaa tgggtctctc
    22321 ccggaactca cattacggcg gctggcgcaa actccccggg caggcaggaa ctggatcccg
    22381 ggataataac ctcttcgagt gtattcgccg atgatgccga tcaggcatca aggatcgggg
    22441 aatgcaggac cgcggtagag accggcctca tatctccgaa gaatattaca gccataggag
    22501 aggtaataga aggcaaggca gccggaagaa ccgaatctcg tcaggtcaca gtctacgata
    22561 tgacaggaac ctcccttgag gagactctgg tcgcggaatt ggccctgaga agatcaacct
    22621 acagaaacac cggctacatc accgggataa tatgatattg ttcatttgaa atagtttacg
    22681 agccagggat agtaggtacc aagagctttc ggggtaaggc aaaaaacaat ataactcccc
    22741 atcgcgacac atgactggat caccgtaagg atatccttcc tgggctctct tctggcgata
    22801 taaccgaggg caccgataca ggcaataaag gcaacagtgt ggtaattcca tccggtcccg
    22861 gtccccgctt cactgatcac cctgtaggct ctaagaacgc agtatgcgcc ccaccccgcc
    22921 tggatgtagg tctcaccgag aaagagaact cccttcagcg ggtaccatat catgggcctg
    22981 aaggccgggg gaagtttttc gaactcgcca agggaagttt cttcgggtct cgcctcgccc
    23041 tggaaaaaac tgctcaccaa attaacgggt ttctggacac accatgagta aaaaagtgta
    23101 aaagttatcg cagcagcaga aattgaaaaa aagaaaagta tcatcggggc cctcctttcg
    23161 gtcaacccat tggatcgggt ctcgacaata ctataacaaa ccatcaaaaa agggagcccc
    23221 gaggctccct ggatatttgg ccttctagtc ggaaagggtt ctcacaagat ccgtcaccag
    23281 gagcttttct ctccagcgat aattaatctg tgcctggttt ccgtcaaaga gactgtctgg
    23341 ataactgctc cggaaacgcc ctgatgcccc tggatccgct gactttcttc tgataacctt
    23401 catctcgatg tccactggag gttcctcctt ttctgacatt tcttccacga catcgataat
    23461 gacataagga taactatttg tcaacctttc tggatatggc atccttccat tccgagattg
    23521 aagctgaggc agccacaccc tggatccagt ccgccgatcc cccgcccctt gcggacagga
    23581 aggggttctc cctcggaata gcccttaggt cagatcccag attttgccct gcagcaagaa
    23641 caaaggacac ctcgttgttc tcgtctgccc tcgagagaaa caatacaaag gcatccggtt
    23701 cattggccat cttttttttc atggaaccag ttgccacttc gacgggaaaa ttctcggcgg
    23761 agaaaacaaa aacatcaaaa cccttgcatc gatggcgttc ccagggaatc tccacgaagg
    23821 acttcactct gtcaattttt ttagacagtt cacgcctttc ttcctggagt ttttggacca
    23881 cggagggaag tttttcctct tcgcatccca catccctgag caagaccctc accatgcgac
    23941 tctgtttttc cagaaattct tgcctgtcca cggtgaaacg gatctcccag tcaggggggg
    24001 aacccctgaa acctgttgct attatgcctc ccaccagacc ggtcgaggac acatggctgc
    24061 ccgaacaggc tatagaatcg tatcccctta tagagattac ggctattttt tcttcttcca
    24121 gccgatccca cttgatcctg accccttcaa attcttcaaa gtcacatttt gagcgttcat
    24181 cccgggttat cgggagatct tcgccgataa ccgagttcgc tttctcttcg gcatggaaaa
    24241 tctcatccca tcctatctcg ccggtgaact tgatatatat ggtgctctcc ttctccccta
    24301 tccagacctt ttccaccctc agcccctcaa aaagatcctc cagggcccgc gaaaaaatat
    24361 gctcgcccgt gtgcattctc gacagcctga ggttcctttc ttcgtcaacg aacgcggaaa
    24421 caatggagcc tgtctccaga gtcccttccc ttattctgat ggtcaggata tctcccccgt
    24481 cgcccttgtg gcagtcgaca acatcggccc tgaatccttc cccctccagt gttccactat
    24541 ccccgggctg ccctcctcct gcgggatgga acactctccc ggagatcagg atctttgcct
    24601 ttttccccga caactccaga atttcctcga ttctgtgcct gttttccatc tctacctccc
    24661 gagtttccat tatgtccatt cacgatgata cactattgaa aagatgccag ggggttacca
    24721 tggaaaacag ggtttgcaaa agagttttga tatcgggagc cgttcaggga gtaggattca
    24781 ggtggcgggc aagacagttg gctctcgaac tgggaataag tggatggata aggaaccgcc
    24841 ccgacggaaa agtcgaggcg gtcttccagg ggccggatat tatggtccgt gaaatcatcg
    24901 attgggccag aaaagggccg aaaggagcag tggtaacgga actctgtcag ttcgactacg
    24961 aatgcgaccc cgttcctttt gactttgtga taaaaagaaa cgcatgaaaa accggcatga
    25021 aaattattta ccctctccga cgactttcag ttccggtttg atgttctcgg cctccgggtg
    25081 gacggtcctc cagagtttta caggaagacc ccatattttg ataaagcccg ccgcagcctc
    25141 gtgatcaaag gcatcccctt cagagtatgt tgccatggag tcaacataga gtgatttttc
    25201 ggccttgagt ccaaccacaa ctgatgcgcc tttgtaaaga cgcaccctta ccgtcccggt
    25261 gacattttcc tgaatagaat cgaaaaaggc atctaatccc ttcctgagag gcgagtacca
    25321 gtaaccgaag taggtgagtt ccgcatattt tagcgacaga tctgcctttg cggctattac
    25381 gtccctggaa agggtcatgc tctcaagcgc ctggtgggcc tgaagaagta ccaccgtcgc
    25441 cggacattcg tatacttccc tgctcttgaa accgacaagc ctgtcctcta tcatgtctat
    25501 cctgcctacc ccatgacccc ctgctatagc gttaagttca gatatgaggg taacgccatc
    25561 catctctttt ccgttcaacg ccacagggat accctcaagg aaggatatct ccagatactc
    25621 gtggttgtcg ggagcttccc agggatccac ggtcattgtg aaggcatcgc tcgggggctc
    25681 agtccaggga tcctcaaggg gaccacattc aatagagcgc ccccagagat taccgtcaat
    25741 ggaatagggg ctctcaacag ttaccggtac cgggatattg ttgaccctgg catactctat
    25801 ctccgcatcc ctcgaaaaat gccagtccct gaccggagcg atgaccttga gatccgggtt
    25861 cagcgcgtta gtgcagacct cgatcctgac ctgatcctgt cccttgcctg tgcagccatg
    25921 ggcaacggcc acggctcctt ctatctcggc gacccatgca agatattttg cgataagcgg
    25981 cctggaaagt gccgaattga gcggatattt accctggtac atcgcatttg acttcagggc
    26041 aggccatacc atcgtttcca caaactcctg acgcaggtcc ataacgtagg cattgactgc
    26101 tcctaccgca agggctttca ttttgacctg gtcaagatcg atattctgcc cgacatctgc
    26161 agtgaatgtc accacgtcat aaccctgatc ctgaagccat ttgaccgcca ctgatgtgtc
    26221 aagtccaccg ctgtaagcca gaactacctt tcccttgtac tccatttcca aaatccctcc
    26281 cctggataaa taaaaaaagg ctcgcccctt ctttcgaagg ggcgagcctg agcccgcggt
    26341 accaccctaa ttgtcatgcc tcccggccga cctctcacgg ctattaaccg tttcttccct
    26401 acttggcctg tgaaagcctt tcttcaggaa tcggctcggg ggctctattc agtcggtctc
    26461 gacgaggaac gctttcagcc tgttgctgca tcccctctct gtcgacgagt acgcgaccta
    26521 ctcttccccg tcatcaccgc gtatcccgcg atacctgacg cagaaaaacc aggtcttatt
    26581 cgtctgactc gtcttaaagt ggttgcaacc atgaccgcac caacctcgct gaattatcgt
    26641 tcatcaatgg cggaaattat acacaggggt tatagcaatg tcaacacttt cgctgaaaac
    26701 ctgtgccgaa accaaaccgt tcgggtgttc tgccggccct tcagctatct ttttcaaaac
    26761 ccgccctccg gagtttttct atggaaggat aatgggtcat atccatgtgt atcggtgtta
    26821 tagagacata accctccgat accgctttta cgtcactgcc atcgtccatt acatcatcgg
    26881 gctgaccgga gatccagtag taggatcgcc ccttgggatc cttaagaggg ttgaccttgt
    26941 ccctgtagat ccggataccc ttttttgtga ttctgaaccc cctgatcttt cccgccggga
    27001 gatttgggac attgacgtta aggagtatcc cctctgggaa ggggaaattt ttgaagcctt
    27061 ccaggattgt tgcgaccgat tcggcggcag attcgtaatt ctcgaccttg tcggactggg
    27121 ccgagttaag tgaaaccgct atggagggcc tccccaggat atgtccttcc atggctgccg
    27181 aaactgttcc ggaataagtc agatcatcag caaggttggg tcccctgttg atgcccgata
    27241 tgaccatttc cgactctggc gcaacggctt cgagacccag tacaacacag tcggaagggg
    27301 ttccctcaca cgcgtaaacc ttcatgcctt ccggatagcc tccgccctcg atctcccaga
    27361 gtctcagggg ccttgtcaag gtaatggagt gacctacgct gctcctttcc ctgtctggag
    27421 ctactacgct cggagagtaa ccatgctctt tcaacaccct ggaaagagtt attattcctg
    27481 gagaaaaaac gccgtcatcg ttcgagacca gtattttcat gattcgggac ccctcaggtt
    27541 caaattatca gataaaggag agtcctgatt atcggactca ttatggcctg gactactccc
    27601 agcaccagca ggatcatcag gacaaagaaa ccgtatcttt caagccagaa gaaccacccc
    27661 atatatttcg gaggaagaag gacataaagc agttttgacc catccagggg gggtatcggg
    27721 atcaggttga aaacccccag gccaaggttt atcaatatca tgaggagaat aaacctcccg
    27781 agggcggggt tggacatgaa gggcccggga acaagtctta cgacaaggcc gctgaaaacg
    27841 gccgtcagga agttccctgc cgagccggca agagagacga ttatcaggtc acgcctcggg
    27901 tgcttgaaat acctcggctc gacaggaacc ggtttggccc agccaaaacg gaaaacaagc
    27961 aacatcagtg cccctatagg atccagatgg gccaggggat tgagtgtaag tcttcccatg
    28021 cgttgggctg tcgggtctcc aagcctgaag gcagcgtaac cgtgacagaa ttcatggaaa
    28081 gtaatcgccc acaggactgc gggcaagctc agcagaagat ctccaagagc cgggaaatgg
    28141 gacactactt ttcacttcct ttcagtatct tgcagggcag gaaatgatcc tgccgaatag
    28201 ttttccttga tccattcgat ctcatcctcg cgactttcaa cttctccgtc aagccttgcc
    28261 attttcagat tcgaaaggat aacacctaca aggggacctt catgataccc catttccaga
    28321 acatcgcgac ccgaaagcat cggttcaact ttttgaagcc gggtaaggta tagaagtatt
    28381 ctccttctta ctctccatct cccggtggct gcggcccaga atattgctgt tacagggctg
    28441 tattcctgga gaaacatcat aatgtgggag ggtttttttt ctcccctgcc ccccaggcga
    28501 tgctcggccg atcccagttc gaatattccc cttataacga tctccctctc tccgggagaa
    28561 agactcagac gatcaagcac cctggtctcc acaccatccg gtgaatcaac caggaccgcc
    28621 ataagggaag caagccattc cttttccccc aggtctggta tatccctcat tagtcttttc
    28681 cggaaagcac caagccttct tagagccctg gcagcaccga tatcaaggat caatcccgga
    28741 aacagagatc tccagaaccc cagatatttg agcctgcgga ccactgaaag ggccttctct
    28801 tccctgaaga caagttctat ctcgctcctt aacctgactc ccgaaagaag agagagcagc
    28861 ccccctttta tgcagttttc aagaagcctg agggtgttgt cctctatcgt gaaatcaaga
    28921 cgctgttcca gtcttatacc tctgatgacg cgggtaggat cctcgacgaa gctcagattg
    28981 tgaagaaccc tcaggcttct gtttcctgta tcatttctgc cgccaaagta gtcaaccaga
    29041 actccccagc tgcccctggt tatagagacc gccattgcgt tgaccgaata gtccctccgg
    29101 tacagatcgt gtttaaggga atcgatggat acccgcggct gtgccaccgg gtattcgtag
    29161 aactctctcc ttgcggtcgc tacgtcgacc tttcgtccgt caggaaaaac tatggtgcca
    29221 gtcctgaacc tctggtggat agcgaccctg cacccgtcct ttacccatga ttcgagaaag
    29281 gccaccgcat ctccttcaac gacgatatca agatcttcat tcggtctggc caaaagaaga
    29341 tcgcggacaa aaccgcccac cacgtaagcc tggaaaccca gacttgaagc cctgtcaccc
    29401 agtttgtaaa gcagggacaa gactttcgtg tcaaggatac cggacatcgt gccggaaaca
    29461 ttttccatcc agggcatctc gggagaaacg gaccttgttt caactggaag tgatctggga
    29521 aaaagagcac ggagaatgtc cgtcctggtg actattccca gaatttcttc gccgcttgtt
    29581 acggggatcc ttccaatgtt tcgcaaaacc atcatcctgt gagcctcggc aacggaagca
    29641 tcggggagta tcgttacagg atctcctgtc atataatcct ctaccagggc cgaacccaac
    29701 ccgtggatcc tcgccttgtc gaggtctttt cgtgtgatga tgccaagaag tctgccatca
    29761 acagacaccg gaagggcagc gtgaccatac ctgatcataa gcctgtaggc ttcctcgact
    29821 gacatatccg gcgttattac cataaccggg cttgtcatga catcctcaac tgttacaagg
    29881 ggctggatct ccttttccag gcgactctca agtctgccaa ggatctcctg cggatcctca
    29941 cccgtgagag taacagaggc agcctgggaa tgtccgcctc ctccgagagg ggaaaggaat
    30001 tttgccacat caagtatctc ttcctttccg cgcgcaacga cataaaccct cttttccata
    30061 cgaacggcag ccagggctac atcggcatca aagtaatccc tcagcctgtg cacgaaaagc
    30121 gataatcctt ctacgtaatc cgggtgcgac atcgaggaaa gaaccacttt ggctccctga
    30181 atatatctct ccctggcact ctcaataagg cggtcaagga tcctgcgctc ggaactatca
    30241 agggaaagct ctatgtgggc agggatggcg gtcatgtccg aacccatact cctgagccga
    30301 atgacggcca tgaaatccct ttctgtggtc gcaccaaagg tgagagcgcc agtgtcctca
    30361 taaatgccca tggcaaaaag ggttgcctcc tggggggtta tctctattcc cttgtcaatg
    30421 agcatttcga caagaagcgt tgttgtagct ccaacgggtt cgatcaggga gaaatcagca
    30481 gttatatcgt cctgaactgc ggcatgatgg tcgtatatat ggacctcgag atcttgtctt
    30541 tccaggatcc cggcaaacga ggctatcctg gcgggagacc ttgcatccac tactatcagc
    30601 gtggaaatct tgtccatttc gacctggctg ggtgtaagaa tattccatct tccagggttt
    30661 cttttaagga attccctcac gttccggccc gccgaacccg agaaacatgg ccttgccccg
    30721 gggtaaagtt ttgatgcggc aaccattgag gcaagggagt cgaaatcagc ccctatgtgt
    30781 gtagttataa tcttcatcag tctctccccc ggcgaccctc aacaggatcc tttttggcac
    30841 acttaacgtc gcgtcgccag tttccattgt aaatacgtct tcaggacaac actcacccag
    30901 gatatccctg atcttttcga tataaccagg tctggcccag acattaattg cttttttgtc
    30961 cgggcaaggc tccattccga ccacttcgga ataacccgaa agagcatctt tcagacacgg
    31021 ggatatttcc ccgtcctgtg acctgacgaa gaatccggtt aacgacgacg cgtcaatgaa
    31081 acccttcgca aggatcatgg ctctttctga aaggtcctga agacctccgt cgttaagaag
    31141 cacaaaatca gccttgtctc tttttgtttc agtcggcata agccttttct ggcgccggtg
    31201 gatctcccgg gcatcccagc ccctggccgc gttccttgag atccggcaga tctcgggggc
    31261 ttcaacgtaa acggtgaggt caatccactc gggaaccccg ttctcgaaca gaagaggtat
    31321 ctcagccaca acccatccat ccagttcctt tacccacctg tacatttcac gccttgccaa
    31381 cggatgcaca gtatcgcata tccaacggta ttcgttgtca ttgtcaaaaa caagagccga
    31441 aatggcggca tgatcgggat atccggatgg atcaagaatg gactcgcccc atctgtctac
    31501 agccgccaaa acaagctctt ccgttctcca gagtttggca actatggcat ctgcactcag
    31561 gactctggcc cctgcagagg cccagatcga ggaaacagtc gacttaccgg ccccaacgtc
    31621 tccagtcagg cctaccacaa gcatggggga tcccctcctc acccctgtct tcggtatcct
    31681 tgaattcatc gggtatctcc cagagaaacc tcgaaaaacc gttcctgaga acgtttccga
    31741 aaaggacccg gcttcttgca gctgtcatat agagcctttc ttcggccctg gtcattccca
    31801 catagcatag cctccgttct tcctcgaggt tttcaggatc atccatacat ctgtaatggg
    31861 gaaaaacggc ttcctcgagc cccacaagga acacggaagg gaactccaga ccctttgccg
    31921 catggagact cataaggctg actttttccg aatcatcttc atcaagatcc tggtccgtca
    31981 ccagcgctat ctccgccagc atcgtttcga gcccttcgcc ggttgcggcg accgaaagaa
    32041 gttccattac gttctcatga cggtcgtgcc atccctccgg atctatcttt gaaagttcgt
    32101 ccctgtaacc gatgtcctgc attatgtaat tgatgacagc aactatatca tcctggatcg
    32161 tcaatatcct catcatgtgc gtggccagtt cggccgcacc ggcggcagcc ttggaagcga
    32221 gacctgaaac ccctgacgct attcccttcc atatctcata agccggaaca gattttcctg
    32281 ctatgtggcc catcaggcgt tcaagactct tcttccccag tcctcttgaa ggtacgttag
    32341 ccactctctc gagagccgcg aaatccatcg ggttcaccgc cagcctcatg aaggctatag
    32401 cgtcctttac ttcccgtctc tcgtaaaaag ctgtccccct tactatcctg tagggaatgt
    32461 cgttctgcag cagcgcttcc tcataaaggc gactcatggc gtttattctg tagaggatag
    32521 ccatatctcc aaaaccatat ccctggctcc gaagtctcat tatctcggac gctacgaaaa
    32581 tcgcttcttc tctctcgttc ctcccgagga gtacgtggat cttttctcct cctccccggg
    32641 aagtccagag tttcttcggt tttctttttg agttgttgcg gattattcca ttagcagctc
    32701 caagaatatt gccagtcgat ctgtaattct gctccaggac gaccactttc gcctttggaa
    32761 aatctttctc gaagttaagg atcatattca tgtcggcccc acgccacccg tagatagact
    32821 gatcggggtc accaacaacc attatcctgg gatctttccc cgaaagaagt ttcagaagcg
    32881 tgtactggga ccggttgacg tcctgatatt cgtcaaccag tatccagcga aacctttctc
    32941 tctcctcctc aaggatctct ccgtcattta gcagcaggtg caggggaaga aggatcaggt
    33001 catcgaaatc ggccgctttc tgcttcgcca gcccctcctg gtatttggga aatatcctgt
    33061 ctacaggaaa atccaggggt cttggggaaa ggctcctgaa atcgcattct gtctttgccc
    33121 tcgatatgct ttcaagtacc cagccaggct caaatcgctt ctggtccaga tcaaggtcct
    33181 tcatgacaga tttaacaagc gatctggtgt cggcacgatc aaaaatggaa gatatttttc
    33241 tgttggcgat cctctcgagg tctttggaat ggcggatcag aaaacgaaga ccataggaat
    33301 ggaaggtccc gatatccatt ccacggatgc ctgttccgag aagattgcaa accctttcct
    33361 gcatctcccg agcggccttg ttggtgaagg tgactgccag gatggaagag ggttccagtc
    33421 cctcttccga gacaagccac gcaaatttgt gtgtaagaac ccttgtcttt ccactgcctg
    33481 cgccggccag caccagaaga ggtcccgagc actcccttac agcttcggcc tgggtgtcgt
    33541 tgagggatcc tgcaagagct gttttccgga ataccgtcat ttgcttttgg cctccataca
    33601 tgcagagaga aaagatatcc attcgtcaat tcctgccccg ctggtagatg ctacctcgaa
    33661 ccttgacgca ttcgggttga gagaaattac gtcattccag aaaagcttca tgtcaaactc
    33721 cagatattcg gcaaggtcga ccttggtcag aatgacggct tttgccatgg atatcaggtg
    33781 tggatatttg agcggcttgt caggaccttc cggaaccgaa catacagcta tcttgaaatc
    33841 ttcccccaga tcaaattcgg cgggacatac aaggttgccg acattttcga taaaaagcac
    33901 ctctacccct tcaagatcca tttccagaag tgctttttct ataaggttcg cctccaggtg
    33961 gcagcctcca tgagtattta tctggatcac aggggtacct gtcagggcta tcctttccgc
    34021 atcccttgac gtcgccacat ccccttcgat cacagcagac ctgaatcccc cttttttcag
    34081 ggtcgcttcg aggagagttg tcttcccggc tcccggggag ccgatcaggt tgaccatcaa
    34141 tatcccgcgc ttttcgagtt cacctcgcaa ccgcatggca attttttcgt cagccgccat
    34201 gacggatttt agaatgctaa tctttcgggg catcctgatc ttccacctcc acagattcta
    34261 tttccatctc catcccctgc ctgatctcta catcactgct cccgcactcc gggcagagga
    34321 agggatcctt ctcccattct ttgcagcacg acctgcacct gagttcaacg ggaagttcaa
    34381 taatctcaag ttccgcacct tccatcaggg tttcccttgc cgcaatatcg aatgaaaatg
    34441 acatgacgtc aggaattacc tgcctcatgc ttccgatctt gatggtaacc ctggttatgc
    34501 gaccccacct gttttcgcga gcctgttgct ccagaagatc gacgagcgct ttgactagtg
    34561 agagttcatg catctacaga catctcctta ccatgttgat cgaaacccgg ttttcatgaa
    34621 aaggggctcc ataccccggg ctgaccagtg ggatcgtaag ggaatttact cccggaatac
    34681 cctgtttcat gatacagatc cctttcggga tcgaactgtc cgctttgatt acaaccctca
    34741 tggaaccgct ttcaccccgg ataacccctt cggaaccttc cagggcacca agtcctccga
    34801 gatcatccag tgatacgacc gctatcggga gaccagtggt attctcctga catatagtct
    34861 gtccattgat atattcattc gaatgcactg aaatcaacct gaattctcca tcaaaggaag
    34921 aagatttcgg tgtttcctcc ggaagcctga ccgagtattt cccactcccc cagtgctcgg
    34981 gctcatccca gtaatatacc ccttctgaaa cctgcctgag cctggagtct ttcctcagga
    35041 tagaatccat ttccaggcgc tgaccttcaa gatcggagga aacgcccatc ttcctggcca
    35101 acccgtcgaa gatctccagg tctgtcagca cttccccggg agaatcacat actttcaagc
    35161 tcctgaacac atactgatgc caccaggacc ctctccagtc aggaccttct tccaaaaaaa
    35221 gagatgacgg aagaaccaga tcacacatcc tggccgtttc cgtcatgaaa aggtcacaac
    35281 ataccttgta tggtattttt gacagggttt cagtaaaaac atttgagccg ggggactgct
    35341 ttgcagggtt ggatccacag aataccgcaa cgtctatccc cggactgaga cctcctgagg
    35401 agtaccatga agaaacgggg agttttctct ggacggcgca ctgccgggta aggctatccg
    35461 ggaacagggc catttcgtct tttccaaaga ccacgccaca tccaggttcg cttagccctc
    35521 cgatcattac cgcaagcgca accagtgaat tgaaagtatc cgagccgctc atgtatctct
    35581 gaataccgta cccgggatag accgcgacag gaccgttttt caggatccat tctgctattt
    35641 caacaacgtc cttttctttg agtgaggttg cctcttccac atcgcggaga tcgagttctc
    35701 cgatcagttt ttcaaaggca ggaaggttct ggactccgac cttccaccgg tccggaagca
    35761 aaccagaccg gtaaagggtc gcacaaagac cgagagccag aactccatcg cttccagggc
    35821 gtatgcgcca ccatttgtcg gaaatcctgg tagtctccgt ttctctcacc tcaatagaca
    35881 ctacccctcc tcctctgttg cggatccctg acaaaagagg aagcaaatgc ctgttggtaa
    35941 ccgccgcatt gcgcccccaa aggaggactc cattgctttt ttccatgacc gtttccggaa
    36001 gcattaccgg ggtggtacca aaactttttc tgagccccgc cccaccggcc gcaccgcaga
    36061 gcgaaccaac cgtagtggaa taccctccaa gggcagcaaa aaaattggga aggaggttct
    36121 tggagaagta tagagaccca tagcccctcg aaaaaaatat cgagagtgga ccattcctgg
    36181 acaaggctct ttctgtcctg gctgcccaga cagaccaggc tttgtcccat gatatttcgt
    36241 cccaccggga accattctta agcagcgggg actttatcct ttcgggatgt gtccttaccg
    36301 attcccagcg cttccccttg tcgcagataa aaccaaaacc gaggggaaaa gtcgggtcag
    36361 gacctatcag aagggggccg gactgtttat tttttacgat caggggacag gcatcgggac
    36421 aatcgtatga acatgctgtc acaaatcggt tttccaggga tgatcacctc ttgataaact
    36481 cgagtaaagg ggaaccaaaa ttaaaaaaac agggtggggc cgcattgaag accccacccg
    36541 cctgccttgt ctcaccagaa gaagcagggg aaacagaaat tattcttcgg ggaccaggag
    36601 cccgtatccc ccgtcttttc ttttgtaaac gacattgacg cttccactat cggcgctccg
    36661 aaaaacaaaa aaggaatgcc ccataagatc catttgcatc gttgcctcct ccggagacat
    36721 cggtctgagg gggaattttt tggtcttgac gatcttttcc agattttctt cgggctccga
    36781 tatctccctg acaaacccct caatctcaaa ggaaacatcc acgggtttca tccttgcctt
    36841 gtccttgaga aaggatttgt gttttttgat ctggcgctca atgctcttga ggcatttgtc
    36901 aaaggccttc ctgatgtctg gcgcgtagtc ctctcccctc atgataaccc cgttgacatc
    36961 tgccgttatt tccacaacat tcattccccg gttgaagctt acctcaacct gggtgtccct
    37021 gatgcgatca aaaaactttt cgagcttgag gagcttcttc tcgatatatt cctgaagatc
    37081 gtcctgcagt tcaacatttc tggttacaaa gcggatatcc atggcccatc ctccaatctt
    37141 cagtgtcgtc cggaaaaact ctctctgaag ctattttaac acctcatgat cagtcgttca
    37201 atgactctac ctgtttactc ttgcgaatca aaaaatcatc ctcttcccgg ctgtttgccc
    37261 tataatttct tttggttgaa aatacctcat ctctcgggag aaaggggatc cacagaaaat
    37321 gaaattttcg gcaagagcat cccgtatgga accatctgca actctcgaag ttgtagcaaa
    37381 agccaaaaat ctcaagaatc agggcaaacc ggtgatctct ttcggtgcgg gggaaccgga
    37441 tttcgactcg ccccccgcgg cccgggaata tgctatcaag gccatcaatg acggacaaac
    37501 ccattacaca caatcaagcg gtctcccgga actgaaagaa gctgtaacag attattaccg
    37561 aaaacgattt ggcctcgatt acagccctgg ccaggtcgtt ataggagccg gggcaaaacc
    37621 ccttctttac gagacactcg ccgccctgat cgatccggga gatgaagtga ttgtcttctc
    37681 cccggcctgg gtcagttacg tcgagcagat aaggctcctg gacgggaaag aattgatcgt
    37741 agacactaca gaaacaggct gcgtgccaaa tatcgccgta accgaaagga tgataacgcc
    37801 agcaacaagg ggcatacttg tcaacacccc caacaatcct accggagcag tatatagaga
    37861 ggatgtcctg cgcgatattg catccctggc gaaaaaacac gatctttgga taatatttga
    37921 cgagatatac gaacggctgg tttacgggaa cacccagttc aggaacattt tggaaattgc
    37981 cccggaggtt tacgacagga ccatcctcgt gaatggtgtt agcaaggctt tcgcgatgac
    38041 gggatggagg ataggttacg ccctggcccc tgaatggcta actccgaaga tcgcatcgat
    38101 ccagggacat ctcacctcga acccatgctc catagcccag tgggcggcac tcggagccct
    38161 gacaggatcc gaagaagaca ccgaaaaaat gcggttagct ttctctgaaa gacgggatct
    38221 tatgatagac atgctgcgca ggatgcccca catctctttc agtaaacccg aaggagcttt
    38281 ctacgtcttc gttgatatca gaaagactct gggatattca ttcaggggga gaactatcgt
    38341 tgacgatggc gatttttgca gtatccttct ggatagcgaa tttctggcag cggtcccggg
    38401 aagcgcattt tttgctccag ggttcataag gctggggtac gcaaattcag agaacgatat
    38461 tcgggaaggc atgatgagat ttcacaggtt cctcgatcag ataaacaaaa aataaaatgc
    38521 ggggcatccg atttccggat gccccgggaa tttcctttca agatcagtcc tcgagtatct
    38581 ccttttcctt ggcggccatt atctcgtcaa cactcttgat gaattcgcct gttgttttct
    38641 ggatggactc gagaaagtca tgcatggagt cctcgcttat gttgccttcc ttttccattt
    38701 tcctgaaggt atcgttcgaa tcacgcctca ggttccttac tgcaatcctt gcgtcctcgg
    38761 catacttttt gacaagtttg gtaagttcta cccttcgttc ccccgtcagt tcagggataa
    38821 caagccttat attctctcca tccacgttag ggtttatccc cagtgaggat gcctggatag
    38881 ccttctcgac agcctttatt gccgtcttgt cccagggagt tattactatt tgccgggcat
    38941 ctggagtaga tatagcacct acctgggaga tgggagtctg ggccccgtag tagtcgacct
    39001 tgatctcgct gacaagcgcc gggtgagctc ttccggtgcg aacaccgaga aggtcttttc
    39061 tgagatgttc gacagccttt tccatcttct ctttcattct tttgatctcg agttcaggca
    39121 ttatcatcac tccttttcaa gatggacaac agttccaacc tgtttgccgt tgatcaggat
    39181 gtcccggagc gtgccttcct tcaggatatt ggcaacaagg atggggatcc ggttttccat
    39241 acacagagag aatgccgccg catccattac tttcaactgc ttctgcagcg cctccatata
    39301 agtaattctg ggaagtagtt tcgcttccgg gttttttgcc gggtcatcat catatatacc
    39361 atcgaccttt gtggccttca atagacaatc tgcagatatc tcggcagaac ggagtgccgc
    39421 cgccgtgtcg gtcgagaaaa atggagaccc cgtcccggca gcaaatatga cgatccttcc
    39481 cttttcaagg tgtctcagtg cctttcgacg tatgaagggc tcggcgatct ctctcatctc
    39541 tatcgccgtc tgaaccctgg tagggattcc aaggtgctcc agaacattct gcaaggcaag
    39601 agagttaata accgtcgcga gcatccccat ataatcaccc tgagccctgc ccagttcagc
    39661 accggtaacc tgggcacccc tgatgatatt ccctccgcca acgaccatgg ctatctccag
    39721 accgctctcc gcaatggaag ctatctccct gcatatcttt ttgatcgcgc ccatgtccag
    39781 cccgaagggt gaatccccgg ccagaacctc acccgatagt ttgagcagga cccttctata
    39841 ggagcccatt tggtaaacca cctccacctg ctttcctcag tccaaaaaaa gagggcggga
    39901 aatttccccg cccttttatc ctattccccg atcgagaagc gggaaaatct tttcacaaca
    39961 atattttctc ccaatttggc tatttcctca acaaccagat ccttgatctt cttttccggg
    40021 tccctgatat atttctgttc catcaggcag ttctcttcgt aaaacttggc gatccttcct
    40081 tctgcgatct tgtcgacgat gtgttccggc ttcccttcat caagggcctg ctgacggaat
    40141 atttcctttt ccctgtcaag atcattctgg gggacctctt cggaggtcag gtaactcgga
    40201 cccgcagcag cgatatgcat ggctatttcc ttgccaagtt cctgaaattc gtcagtcctg
    40261 gcgacaaaat ccgtctcgca gtcaagttca acgagagtgc cgatctttcc gttagtgtgg
    40321 atgtagctga aaatacgtcc ttcggatgcc tctcttccgg ccttcttggc ggccttggca
    40381 agaccctttt ctctcaggaa atcaacggct ttttcaagat cgttcccgca ctcggaaagg
    40441 gctttcctgc aatcaagaac ccctgctcca gagcggtatc tcagttcctt tacggcatca
    40501 acatctacag acattactgg gcctccttcc agccttttct ttcgagaagt tccttgtcaa
    40561 cgatgacagt ctcaacctca ccgaattttt catgggcctc ttcggatact ccgttttgct
    40621 ggcccgcatc gtcttcggca tcagtgtcat cagcactatc agcacatacg ttgtcggttc
    40681 cctggtgtcc ttcgatcacc gcagaggcca taagtcctac aataagtttg atagccctta
    40741 tggcatcatc gttacccggg atcgggaaat ctatcaggtc ggggtcacag tttgtatcta
    40801 caatggccac aatgggtatg ccgagtttcc tggcctccag aatggcaatg tcttctctct
    40861 tgggatcaac tacgacgaga gcatcgggaa tgtccttcat gtcctttatc ccgagaaggt
    40921 atttctcgag tttctccctc tccttggcaa accctgctat ctccttcttg gtccacttgg
    40981 tggaaccttc aaggaatgac ggtgatccaa gttcgaccat tctcgccacc cttttcttga
    41041 tggtcgggaa gttggtcagg agaccgccga gccagcgatg agtaataaag tactgtccac
    41101 aacggacaga ctcttccctg atggtctcct gggcctgttt cttggtgccg acgaaaagaa
    41161 cgctccctcc acccttcgaa acctccctga gaaagtcata ggccttctcc aggcccttga
    41221 ccgtcttctg gagatcgaca atgtagaccc cgttcctctc cgtaaatatg tagggcttca
    41281 tcttggggtt ccagcgcctg gtctgatggc caaagtgcac tccgcactcc agcagctgtt
    41341 tcatgctcac taccgccaaa ttcttcgcct cccgttttct ggttttccct ccacgacctt
    41401 cacccggtcc cgggatccgc acaagggcgg acaccacccg ttcccgtcgg atcgtgtgcg
    41461 tgataagggc caagggggag tatatcacag cttgagattg accggcaagg ttttgtggaa
    41521 taggatatac ccggtatacc tacttttgct tcaagtcctg gagggaagat catggcgaaa
    41581 acatcgttga tcgaacaatt ggaaaaaatg gatcccgcta gaaaggcaat gctgaacaga
    41641 ctgaaaaggg ttgaagggca gctcagggga atacagcgaa tgataatcga ggataaaccc
    41701 tgccaggata tcctcctcca gctttcagcg gcaagaaagg caatgcagaa tgcctgcatt
    41761 gagatcctga aaagctacgt caagcgttgt ctggctgaat ctcgttctcc tgacatgggc
    41821 gaactggaaa cccttatcgg aacccttatc gacatagccc ccgtatcacc ttccagcgaa
    41881 gtcgaatcct gaacccgatg ctttgacctg ccccgtcaat cgagttccct ggggagcatc
    41941 atccccagga actctccggc gggaaactct ttaccggtta cagaatcaat caggctctca
    42001 cctgaacgta tccatatctt ccagcaggaa cgcatatccc tgtctctggc cataacatgg
    42061 gaagagcccg caagggccct ccacccctcc ctgccctcgg aaacaagagc gcactccgcg
    42121 atacgtaccg ccttttccgg agccaggttt acgggtagtt tcctgccttc agggttgcct
    42181 tcggatatgg caggcatatt tttccttaag gactcaactc ttccacctac cgcctcaata
    42241 acggctacaa ctgtcttata ctccggcctt ttcaacatac ccgtggtgaa aacttctact
    42301 ggaacaaggt acagaggctg aagaaaaaga atatctccct cgatccattt aaccgagggc
    42361 agaaaatgac cgctgaactt ttcatccaga ctcatccagg gacctcccaa aaaaataaaa
    42421 aagcgacctc atgccgggga cccagcttgc ccgcggcacc cgaaagaacc atataccgct
    42481 gctcccttcc gggcctgacg gggttttatg gtctttcgtc gcacgggact gggtccccgg
    42541 caaaaaatcg ccaaaaatgg cggagaagga gggattcgaa cccacgggac gttgccgccc
    42601 agacgctctc ca
//
LOCUS       DAOTIQ010000007        40205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1053_length_40205_cov_7.129340, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000007 DAOTIQ010000000
VERSION     DAOTIQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 40205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40205
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1053_length_40205_cov_7.129340"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="P5541_02040"
     CDS             <1..340
                     /locus_tag="P5541_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRV70785.1"
                     /translation="VSGNDLDKSVLFDIFHGSLRQAPMIRQCPVTMECRVVETLELPV
                     DILFIGEVAGVWSEERFLTGGSPDIEKVKPFCLTMPDNRYWSIGGTVGRAWSDGEGLK
                     GKLPKVGGGS"
     gene            342..1091
                     /locus_tag="P5541_02045"
     CDS             342..1091
                     /locus_tag="P5541_02045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV70786.1"
                     /translation="MNWLSYTSLAWTERILAPPGDLAEEAQIYIEAVKRNSGGKARTM
                     LHLGCGAGGHDFHFKGHFEITGVDLCGEQLALARDLNPGVRYIQGDMRNIRLDRKFDA
                     VVIPDSIMYMSTVEDLDKALLTSATHLVPDGVFLAVVHPKELFRNNNFVYSGSDGDTQ
                     VTVFENDHVVSDSTYEAAIVFLIRRETELQIYNEVHTLGLFSNSEWMRAFEGSGLVLN
                     EEIALNDLYDHFLAEEGEYRLKAFVCEKRRQ"
     gene            1278..1682
                     /locus_tag="P5541_02050"
     CDS             1278..1682
                     /locus_tag="P5541_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRV70787.1"
                     /translation="MPKLEKVEGIGGSYAVKLQNAGVATTDALLKMGSTPKGRKELAE
                     RSGISEKMILKWVNQVDLFRIKGVGEEYGDLLEASGVDTVPELAQRNPENLHLQMNKV
                     NEAKKLVRQLPSVKQVAGWVEQAKKLPRVINY"
     gene            1908..3698
                     /locus_tag="P5541_02055"
     CDS             1908..3698
                     /locus_tag="P5541_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRV70788.1"
                     /translation="MDSSILLVLAVLAVTVFFLVVDVVRIDVVAIVCMLALGWTGVLS
                     PKEVFSGFSSNAVMAMMGVMILGQGIAGTGIMDLFSAAVIRKAGTDRKKIIGLMSLSV
                     GLLSGFVQNIGAAALFLPGILNISRREKIPPSALIMPIGFAAILGGTLTMIGSGPLIL
                     INDLIRDFGLEPYTFFSVTPVGVLLLIAGTSYFLVFGKYILPGQDSGEVPLSVQDRII
                     EAFHLPHHIGHYRIPGGSTLASTTTEESGVWDRYRVNVLGISEGREAEYAPWRETIFQ
                     VGQELALLGKEEDVAAFASDHGLIPVDEPGRFKGLNDPVRAGFAEVIIPPRSEMLGKS
                     IRQFSLRKRYAVEPVMLFSKGEEIRGDLSDHQILPGDILIVHGLWEKISALKNETDFV
                     VATPFVAETKVRTRTWAALACFLGAITLVLTGAPISLAFFTGAAAMVLVGVLSIQEAY
                     NAIEWKVVFLLGGLIPLGTAMQKTGAAAFMAEKLMALVQGGHPLLMVLTVSLLSTVFS
                     LLMSNVGAIMVLAPLVMNMGFMVGMDPRPLALLAAVCAANSFLLPTHQVNAFLMSSGG
                     YRNADYLKAGGGMTLIFLLVAVAAFQIFYF"
     gene            3778..5229
                     /locus_tag="P5541_02060"
     CDS             3778..5229
                     /locus_tag="P5541_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV70789.1"
                     /translation="MLLKHFFTGKIAHSSYILAGKNTCAVIDPQRDVDIYIREARSMG
                     VDITHILQTHLHADFISGHMDLARATGASIYVAKSARCTFDHISLSEGDTVEIEDMAL
                     QVLETPGHTPEHLSYVVVDRSRSDSPAGVFVGDTLFVGDVGRPDLFPDMAQELAGELY
                     HSLHNKLMELPDYVEVYPAHGAGSLCGRAMGAKWTSTIGYERRFNPALQIGDRKEFIR
                     SLTEDMPPAPDHFSRCSDINRHGPALVSDLPVMEELDPKQFLEKMNDPGTVLLDARSY
                     FAYAGQHLKGTWHLDLNGNFPTFAGWVMPLENDILLVADDYKSAQMANTWARRVGVDR
                     IVGYLDGGMANWTAGGLPSSHIEVISAGDLHEMIDENASFILLDVRAPLEFEDSHIEG
                     AVNIQVSDLRTRYGEIDREKPVVVMCSSGNRSSLGASILKQHGFERVMNLAGGMSGYS
                     AAGYVRECRACMNPHGSRFFTTFTEVKKHWDRS"
     gene            5240..5755
                     /locus_tag="P5541_02065"
                     /pseudo
     CDS             5240..5755
                     /locus_tag="P5541_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
     gene            5780..6346
                     /locus_tag="P5541_02070"
     CDS             5780..6346
                     /locus_tag="P5541_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRV70790.1"
                     /translation="MTSGSVNKPKKDIPQLLWGLAFGIAFGFLLQKGGVTKYDIIAGQ
                     LLLKDFTVVKVMLSAILTGMVGIYFMKSRGWVELYPKSGSLGMNVIGGLIFGAGFAIL
                     GYCPGTIAGAIGNGYLDALVGGLAGIITGAGIFAALYPRLNDGILKWGYYGKVTLPQV
                     FKVSDRAVVIPVVVIIVLTLFLVEFAGL"
     gene            6399..7079
                     /locus_tag="P5541_02075"
     CDS             6399..7079
                     /locus_tag="P5541_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRV70791.1"
                     /translation="MNTLVSLLAGFLLAVLLYVSFKIIRRPGSSRGRETTIHSSIQQL
                     KSIGQLSVFKVLTKEIVTEVDHSWGEIGKKYFSWILSNRKMAMIFEFVIDFRYDLRSP
                     DFQIIEESEGSYLLRMPSCVYETSIRDIQFYDEQKCKLLPLLLPDLLNTLLGDGFSEE
                     DKNRLKDAAKRAAESQAESMIAGLCPDVEQSAKQTLQSLAKAFGAENVRFEFPHAGEK
                     GATRDERE"
     gene            complement(7087..7704)
                     /locus_tag="P5541_02080"
     CDS             complement(7087..7704)
                     /locus_tag="P5541_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRV70792.1"
                     /translation="MPKRLSRAFDFIALCLMASTAWFFAGCAFAVEVVGSSTQAKGRV
                     LVAFSTSKGSTGEVAETIASEIRSAGWEAEAVNLNDVHLPEEFNHVILGGPVYYGKIR
                     EVKAFVEKYEEALRDRLTGAFAVGMAFAVDDEEQIAAGRKALDDSTAPLRPAHLGYFA
                     GRIDPGKLSLLEKAAIKMVKSPVGDFRDWEETRAWAREVVKSLDQ"
     gene            complement(7736..8545)
                     /locus_tag="P5541_02085"
     CDS             complement(7736..8545)
                     /locus_tag="P5541_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRV70793.1"
                     /translation="MELTSSVVLDWITLYALKLVAALLILVVGRIVAKGIRSLIRKAL
                     SKWNVDVTLVKFIATLSYFSIMAFVIVAALAQIGVQTASFVAIVGAAAFAVGLALQGS
                     LANFASGVLIILFKPFKAGDYVEGGGVAGTVEEVSIFTTVLRSPDNRVIIVPNGKIMA
                     DNIVNYSAREIRRVDLVAGVSYSDDIDKVRKVLEIILDEDERVLKDPAPTIGVLELAD
                     SSVDFAVRPWVRSSDYWGVFFDLQEKIKKRFDAEGISIPFPQQDVHLHQAE"
     gene            complement(8657..8797)
                     /locus_tag="P5541_02090"
     CDS             complement(8657..8797)
                     /locus_tag="P5541_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70794.1"
                     /translation="MKFLKDKFQIPATRMIPAGLGMLAPVASNRTEGGRANNRRVELL
                     EF"
     gene            complement(8794..9039)
                     /locus_tag="P5541_02095"
     CDS             complement(8794..9039)
                     /locus_tag="P5541_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRV70795.1"
                     /translation="MTVTSEEMAKGIEESGRIALYGIFFDTDKTDIKPESVPTLEEIA
                     KLMKALPELRVLVVGHTDNTGDREYNMGFPAGELNRL"
     gene            9185..9622
                     /locus_tag="P5541_02100"
     CDS             9185..9622
                     /locus_tag="P5541_02100"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV70796.1"
                     /translation="MKVIEAGVFVQASSEKVWRVLSDLGKYPEWNPFILMISGELEEG
                     ARLSVKIGLPEGRPMTFSPVVRVAKPGVELRWLGHLWFPGLFDGEHSFRIDEAGKGHV
                     HLMQSEHFRGILVPFLPASFFERTRQGFEEMNRALKERVESSL"
     gene            complement(9619..11001)
                     /locus_tag="P5541_02105"
     CDS             complement(9619..11001)
                     /locus_tag="P5541_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRV70797.1"
                     /translation="MNPEGWVSLLPVTVLLVAVIVTRRALESLLIAIAVGFMLKDGLS
                     FFPSMLDTVYSVMQDPTIGFVILLCSVMGAFVAVLDHSGGTKGFGDWVSSRVSTRRGV
                     LLGTWLMSLFIFIDDFLISLTLGICMTPSADRYKVPREMTAYVVNSSAAPVASLVPIS
                     AWAVFIAALLEKYLALPAGEGLALFVRTIPFNFYAWFAFFMVPLVVTGIVPVIGPMRQ
                     AEERVRRTGELAPPGSELISMRKPHDMSEEGPKAMARNLVLPVLVLIAGTMWFGVNLF
                     KGGVIALSFCCAFYRIQGIMPVARFTETCLRGIGNMLFPMVFTCLAFTFNYVNQELGL
                     IPFIIGKATPLLSPALFPLVIFVILGFVAFLVGISWSLYLIAFPLVIPLAQALGADLV
                     LAVAATVAAGTLGQHCCFYSDGTILVSASTGCDNIRHALTQLPFAMIAAGLACVGFLV
                     AGFLSTQGFH"
     gene            11145..12614
                     /locus_tag="P5541_02110"
     CDS             11145..12614
                     /locus_tag="P5541_02110"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRV70798.1"
                     /translation="MGTDARSRLTVLHVTPEMTPVSKVGGLADVAGSLPGALQRLGVD
                     ARVFTPSWPGVAERLRDLGARAISGPDRICVPFGGRKVCSDFSVVDLAGTSLCLLEDD
                     ELFSNPSIYPEKLDPLSAMPFAFLSLAAVEYSLSNEWKPQILHLHDWPSSLVPAALKW
                     HRVYGVAREGPRTVLTIHNIAHQGILPLSALDEWGFGPEALTLDGVEFYGSANLLKGG
                     ITASDAVVTVSPNYAREILSPEFGEGLEGVLEYRKSKITGILNGIDTASWDPWRDKAI
                     PANFRTGDMKGKQTCRAVLLSECGWVGFSGPLIVSVGRLVRQKGLELLLHALDEILSM
                     GARLVLIGTGERELEERFSREAIRRQDGLHFRRAFDEGFARLAYAGGDIFIMPSLFEP
                     CGLSQMISMRYGTVPVARGVGGLVDTVSDADAGPRGTGFLFDDFTPEALMCAVKRALT
                     HFSEPRSWSQLRKRCMEKDFSWDLSARSYMTLYSSLSDQ"
     gene            12642..13922
                     /gene="glgC"
                     /locus_tag="P5541_02115"
     CDS             12642..13922
                     /gene="glgC"
                     /locus_tag="P5541_02115"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRV70799.1"
                     /translation="MIYGKHGRVLAIILAGGKGERLMPLTKYRAKPAVPFAAKYRIVD
                     FALSNLVNSGLFSIYVLVQFKSQSLNEHIERGWQFGGALRDRDHFVTLAPAQMWRGEH
                     WYQGTCDAVFQNLHLVTLYDADHVCIFAADHIYKMDVEQMLDYHMARKANVTVAANVV
                     PRSDAWQFGCIHTDEAGRIIDFVEKPKTPPEIPGKPGFTYVSMGNYIFERETLEEAVI
                     DDAQNEASSHDFGRDVLPTLHKKCNVFAYDFSTNVLPGKDRPYWKDVGTIKAYWEAHM
                     DLCKHDSDMTLFNPKWPIRTVSFSDPPTYSYPVGGQMCSIIGSLRAEGSRVLGAIVHS
                     SVLSRNTLINSGAIIEDSILGQGVEIGENTRLKKVIVDAHNRIPPNSVIGYDLEEDRK
                     KYHVDEESGVVVIGMPAMQLRKNIEIPFIPPLER"
     gene            14106..14507
                     /locus_tag="P5541_02120"
     CDS             14106..14507
                     /locus_tag="P5541_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV70800.1"
                     /translation="MIQISEASSLAFHGMGLLARSGGRLSVHEMASLTGSSEAHLSKV
                     FQRLSRAGFVSSVRGPGGGFVLSRPAEEITLLDIYTAIEGEPSMDTCFLGSGMCPFPR
                     CIFGEVIPDIMKRLMDYLSGTTLADVVRDDS"
     gene            14873..16084
                     /gene="trpB"
                     /locus_tag="P5541_02125"
     CDS             14873..16084
                     /gene="trpB"
                     /locus_tag="P5541_02125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRV70801.1"
                     /translation="MRSMRKEDEARKGYFGTFGGSYVPEQLQPRLDELDAAFVSAMED
                     DLFAKEFTRLLKEYVGRPSALTYCANISREYGGGRLFLKREDLNHTGAHKINNTIGQA
                     LLARRMGKKELIAETGAGMHGTATATVAALMGMKCTVYMGEVDVHRQAPNVSRMKALG
                     AEVVSVTHGQKTLKEAVDAALEAYVLNPETFYLLGSAVGPHPFPTIVRQFQSVIGKEA
                     REQFLEREGKLPDHVLACVGGGSNAIGIFSGFLDDRGVGIMGVEPAGRGLETNEHAAT
                     LVAGKPGVIHGFRTYVLTDNDGEPAPVYSISAGLDYPGVGPEHAFLMESGRASYVSVT
                     DDEAVDAFRILCRLEGIIPALESSHAVAQALKILPELSGDKTVLVNLSGRGDKDLDSV
                     LPLLGLQGEVS"
     gene            16325..17311
                     /locus_tag="P5541_02130"
     CDS             16325..17311
                     /locus_tag="P5541_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV70802.1"
                     /translation="MKNDRARQAERVSWVGLGANAFLALLKLSAGFAGNSAAMIADGV
                     NSLSDLGTDIVAVVSFRITGRPIDRSHGYGHGKFETLATAIIGSALLAVAVGIFFGGG
                     ERILTVLRGGTLPRPGLVALAAAAVSILIKETLYIFTRRAALSLSSDALMAKAWDHRS
                     DALASMGAMVGIGGAIFLGPKARVLDPLAALVVAAIILRIAFPITVKSLSELLEGSLS
                     RENEEEIMQAISEVPGVEGAHHLRTRKVGSDVAVEVHIIVARDLGITEAHNIATEVES
                     AIRALHGEGTFVSVHPEPESGDLQGQVPFDDGHNGMEDGPGHHERVPDDLHE"
     gene            complement(17335..18138)
                     /gene="arsM"
                     /locus_tag="P5541_02135"
     CDS             complement(17335..18138)
                     /gene="arsM"
                     /locus_tag="P5541_02135"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRV70803.1"
                     /translation="MKDIRETVRKKYAEAVRKNRSCCGSGSSCCASGSDPITEGIYQG
                     TSKDRIPDAVLGQSLGCGSPVEMAGLKPGEAVLDLGSGAGLDAFLAGGEVGPSGKVFG
                     LDMTDEMILAAESNRVHWGLDNVEFLKGFIEEIPLPEGSVDAVVSNCVINLSPDKDAV
                     FREIFRVLKPGGRISVSDVVFLRPVPEEIRSSIDAWAGCIAGALGMEEFAGKLKAAGF
                     ENVRVEPTRVYEFTPEQLQDMFPDMQGVADIDAKDVAAGAWISAVKPLF"
     gene            complement(18135..18584)
                     /locus_tag="P5541_02140"
     CDS             complement(18135..18584)
                     /locus_tag="P5541_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV70804.1"
                     /translation="MARNLSLLDRDRASCCGVTLAQCHAIVEIGRAEVATPSFLATTL
                     RLDRSTVTRLTDSLVEQGFVVRSQDKVDRRSVTLSLTEKGLRFFEGTENSMNSFFLSL
                     IERIPENHRNVFLSGLGSVVEALERGECVCFDLERLLKGRTEGGCAF"
     gene            18803..20065
                     /locus_tag="P5541_02145"
     CDS             18803..20065
                     /locus_tag="P5541_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70805.1"
                     /translation="MVSEMFRIRKETLDEINCFLLDPGNPDVQAIHRVVAKYGTPTEI
                     NAKAKEARCLSNLMGRLERMGSPYLENLKWLVAERDRGAFIPVSQYRKKILGDEATGK
                     TFRESTSVTLEISALQYFPFLIAEAEQAIDGKEIMPGRYIRVRKMKEQESDQGDILAV
                     AAAMQIVGASYVETLDTKGTDGSNIHLGGPETITGYFGGIGQPNDHALAWVDELLHYY
                     TNYGINEVLNINPGTVLLGYLLYRLGVDVRFKISVFMGNDNPYAVLWTLMMAKLFARE
                     DGSTPLIGFNLSNSVDAEALMACADIRRRLGLEDKVRIEHHITETYRSIVRQPYNRRE
                     DLLKVARVYPNISAKHEGGDPEVEQTIAHPSDILDYFRDKEEVIASGDMDNLKNNYLK
                     KHDATNRTAEELTRAGLSFLAASRLHAR"
     gene            20123..20743
                     /locus_tag="P5541_02150"
     CDS             20123..20743
                     /locus_tag="P5541_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRV70806.1"
                     /translation="MTESLKGINYSVRVSARARRVTLRIAPPEGLVVVIPRNFSKKRI
                     PSLIDEKRQWIEKTLSKVAPQAVTASGTLPPKEYFFPSLKKSLGLEGPNAVSRLRSIA
                     DSHLLPRARVLSLKTGLRPASFTFRNQKRIWGSCNSKRKISLNQKLLFLEPPLVDYII
                     LHELCHLEYRDHSSSFWTFLSSFDPHFRDHRREIRSAQWTIPVWAY"
     gene            20799..21566
                     /locus_tag="P5541_02155"
     CDS             20799..21566
                     /locus_tag="P5541_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV70807.1"
                     /translation="MAEKVFRRVIQIDEDKCDGCGQCAEACHEGAIQIIEGKAKLVSD
                     SYCDGLGDCIGECPQEAISFIEREAEPYDEEAVKENMGSKKEQETLPCGCPGTMARSL
                     EGILETHCDASSKPQLRSEEAVSSELANWPVQLRLVPENAPYLKGADILLAADCSAAA
                     SPDFHGRFVRGRILLMGCPKLDDADYYREKLARIIRVNDSPSITVVRMEVPCCGGLAQ
                     LAEAAIDEARVDVELRTVILDSRGKLVEDETVKFRFQ"
     gene            complement(21662..23248)
                     /locus_tag="P5541_02160"
     CDS             complement(21662..23248)
                     /locus_tag="P5541_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV70808.1"
                     /translation="MAEQYGSFYPWQVKAWERIRNRDAVLAAPTGSGKTWVAYLWSGL
                     MDLDGHIWPPDTRRIIFTAPIKALSNERYLELRRMGLDVGIETGDFKKNENAPVICCT
                     QEIYTLKYAGSPGLRVVIDEFHYIFDDPDRARTYIEGIVSTNPDSPILVMSATFGGAR
                     SVGDYLGEITGRPFTVYEGQERVTKLVFTPKKPATLERLHDALVFCFSQKGAVDLAYK
                     IARRRPRKGKAHRQRLYELAEILDVRDVHMPLLRGVGVYHGGMLPKEKLLVESAFRER
                     ILDVVCGTNALALGVNLPAEYVIFAQLVRYHDREPISRNEFLQMAGRAGRKGLFETGY
                     VTWLNRSPQESKGFDTGKVFRQLTAAPPERASVTLRPDYGRLLKGRRTISDEAEYIAD
                     FSLPHGDERIVEGELRRGLKFIERSIREMVTPDRRERFREILADIWYGEMEIGENLEM
                     ACLFLENGKADALTAAQVINLFEKSYLKSLLKVKRFSNHLPPGYEITGMRYLDRTVEE
                     IDGTIYGFEEKIQEIEDSSL"
     gene            23407..24036
                     /locus_tag="P5541_02165"
     CDS             23407..24036
                     /locus_tag="P5541_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV70809.1"
                     /translation="MRDKEETRKKILEAVGEILSEEGFGGIGVNNVSRRAGVDKVLIY
                     RYFGDLPGLLREFASGGDYWPSMEDLVEKAEGDNRPPGASELAVALLKGYIRELRDST
                     SAQELLLGELVSRNELSEASARKRERQGADLIEALVRSAPEAERVDVAALGALLSAGL
                     TFLMLRARTSEAYLGIDISSEEGWGRIERAMEELVRGVMGDDKLEGFQY"
     gene            24091..25284
                     /locus_tag="P5541_02170"
     CDS             24091..25284
                     /locus_tag="P5541_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HRV70810.1"
                     /translation="MKRPVLILISAIIMTALGCGTALGAGFALYDWSARGNALGGAMT
                     GRADDPSAIAFNPAGITQIPDSSMMAGLAFIMPGGTLTEIGGDSVGMIDHTFIPPHFY
                     YTRQLSDRLWFGVGLMTRFGLGTDFPPDWYGRYNSYYANIQSVSINPNLAWKVNDRLS
                     LSVGVEALWFEYGARRFIGTDFRLLGDDIGYGWNVGLHYKLDEATRIGLHYRSQVKLT
                     ISGTADIQGVGSTDAWGDLTLPEMFLFGLSRQVTPKLNVEVGAIYTGWSSYDRLAIHF
                     ENDFGMLLNPSTSVKNWKNVWRYQLGLEYRYSPEWTWRFGYTYDQEPSPDGTVDFQAP
                     MSDRQLFSIGFGYTKNGRTWDLAYTYLLADDRTIAGRPGDGIPNSRTSNMDAHIIVIS
                     YSMRF"
     gene            25590..26537
                     /locus_tag="P5541_02175"
     CDS             25590..26537
                     /locus_tag="P5541_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70811.1"
                     /translation="MELDFIKGHMGGNTILLFNGRQVRRESHVLETALTVLDRKRLYA
                     DQAAFLYPSGKANTIRALVVDITNRNFIPACGGFTQVLGKALVDTDLVKWFKVKTAPQ
                     EFEVTLELESGPSTVTVHKTKGGKFRKAESDMTSFARMLAGDGVMPVDLDGIQAFKSG
                     YYLVQEAPKLKEHYPEADFEEMNTAAVKAISEAQAFFHQKGYSSTLHFSLFDRSPAHK
                     GHLRAVFPHGVLTGNIEPTCGTGSTALALALLLTGEGKDLGLVKGGRIKVRLETGGGP
                     ALGGPDITTVVVEKKGEEVTRAFFSHSLVEITAKGTVFV"
     gene            26698..27720
                     /locus_tag="P5541_02180"
     CDS             26698..27720
                     /locus_tag="P5541_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV70812.1"
                     /translation="MTSDWTRSQQSVSGFERAMALAGAATLKEALPICLEALIGSSEG
                     SLDCGWVYLVPEKAEIFRLRCSIGVSDPFVEANASWAPDSVHGRAIFRDTPWYGSFSE
                     TLNGQDPLLASEGLHAFAILPLFRGSSVAGCFCLASHDSDTVPQHVKRDLEALSRWFD
                     KTIARVQVSESRIRESWELLSLFDAMAEMVFAFDLDGEIVWINRTAREELGLDNNGRI
                     HLLDLHPDDTRPEAARIFRRMLSREQQTSNVPYAKRDGSVVHATTLGVPGRWKGEDVL
                     FGISRIHQTGGRTSVEAAAEVSSLERDIARMAMVLDERLSRIEEALMISPESSASRLW
                     TGSIKE"
     gene            27717..28577
                     /gene="dapF"
                     /locus_tag="P5541_02185"
     CDS             27717..28577
                     /gene="dapF"
                     /locus_tag="P5541_02185"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRV70813.1"
                     /translation="MNTSGEGILFTKMHGNGNDFLIIDNRLGEIPGHELPDLALRLCR
                     RRTSIGGDGLMAVERSDRGDIRMRLFNCDGSEGEMCGNGARCLARYAFEKGMAPARMT
                     IDTLAGPVVAEVEGVFATLDMGVLETASALRGQVLILSGERLVYSQLNVGVPHTVIFQ
                     DPGDERAEMDLARIARAFQSDRERFPQGTNVNFMKIRTTGEIDLRTYERGVNDFTMSC
                     GTGSTAGAVAAWLEGKVSTPVEVRNPGGVNTVLIDEEGPGRVRLFLKGQTTAVAEGVV
                     SGECLQIREP"
     gene            complement(28881..30005)
                     /locus_tag="P5541_02190"
     CDS             complement(28881..30005)
                     /locus_tag="P5541_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV70814.1"
                     /translation="MKIEPFGVEQWMNRWETLAVNNIAETCVDSLLLYELLEMSGRKE
                     EVMVDLLGTRMTYGDIKGSERLRGLIGELYEDRDPERVLIMNGGSAANFLALFTIVGP
                     GERVISVWPTYQQLTSIPKAFGAKVDILQLRPENFWLPDTDELEKMAESGVDLICLNN
                     PNNPTGSLMDRETLERIAVIARKHGAWVLCDEAYRGLVHEPGVSVSSMADIYEKGVST
                     GSMSKVFSLAGLRSGWMTGPREFIEEAFSRRDYTTISCGPLVDALATVALENRDKITE
                     RNLKIIRESVGLLGEWIDSEPLFHWVKPTAGTTAFVGYDLDIPSDEFCVDLLGTTGTF
                     LLPGSCFGIDKRLRFGYAYGTDALKRGLESLSGWVRERES"
     gene            30204..30956
                     /locus_tag="P5541_02195"
     CDS             30204..30956
                     /locus_tag="P5541_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV70815.1"
                     /translation="MSSLEKSLMILKRLGDSPYELNLSELASHVGMGKSGAFKLINVM
                     KKHNFVIQDPSSKKYHLGPIMLRLGNVYSRFKGIEQIAKPLLFRISRSFQETTYLTIW
                     EGDRAFPAYKNSVKDGIYEYNDFIGMSLPLNSGASAMVLCAYQDPGHIREILGSMKLE
                     KRTSKTMTDLDQLMDEYRIIRERGYALEDETFSLGILSIAVPVFDKDGRVWSCLALAA
                     PKAHATDGNVDEWIQALKEGAEELSYNLQFRH"
     gene            30995..32047
                     /gene="dctP"
                     /locus_tag="P5541_02200"
     CDS             30995..32047
                     /gene="dctP"
                     /locus_tag="P5541_02200"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV70816.1"
                     /translation="MGGQTRKKGEIAMKRNIAFAVLAVFVLGCFIVTGAATPSLAKDK
                     LTLKVAGISAPEYRGTKSLYTIKDIVEKGTEGRIILDIFPANQLGDYTQVFEEIRRGT
                     IEMGLIFLPSQFDVMLEVGSLPFLAETGEGMQEQLSPGSYVYSIIEDSLDKLGVRLLR
                     IYGDGFVGVGLARKPKDPVKAGTPKDAMIRVAPLAVYKETAEDLGFRTASIPYADTYS
                     AIQTGVCDGWIGGSSQINYLSFRDVIKYYIPYNCLFDQTGYIIGKDIWNKISPEDQKV
                     ILDAVNEQADKSFADSNAEDVEYQEKLAEAGVEIIPISGSEMAELAEYIRNTTWPKLE
                     STYGKETLDKIKESLQ"
     gene            32108..32629
                     /locus_tag="P5541_02205"
     CDS             32108..32629
                     /locus_tag="P5541_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV70817.1"
                     /translation="MNLIGSIKRSLFWKSILGLQRILLVLTSLFVVLIMCAEVVLRYV
                     FKSDLFGIEEIVVIAAFWLYFMGSSYGVFEKSHVKADIIPQMLRPRARALLSVLVNFT
                     VSALCILFTVWAVGMVRYSITWMPRTTGLRIPIYISQSSVLLGYALMSMYSIVYFIED
                     LFVFVSMGREREA"
     gene            32723..34027
                     /locus_tag="P5541_02210"
     CDS             32723..34027
                     /locus_tag="P5541_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV70818.1"
                     /translation="MAITVALLLIIITLLLGLPVPFCFMLSTTFMVVTHGYDPSFLLP
                     YGFSQMSSIVLLAIPLFIMVGGMMDRGGIGTSLVNFVEIFVGRIKGGLGVVAVVSCAV
                     FGSISGSCAATLSCIGSIMFPRLREKGYPMGHSAALVSCAAPLGLLIPPSSQMILYAW
                     VGQQSVLACFLATVGPGIVLMILLSLLNLIMLRKDPNIEVVPVMSRGEKLSMFRDRGK
                     RAAPALFMPFIVLGGIYGGIMTPTEAAAMAVLYSIPVGFWVYRGLNRGNFIEVIIETA
                     TTTGVVMLMMYCIMMLSRIYVQERLPDMIINTLTAVSTNRYVILFMINIFMIIIGMIM
                     DDVSAMLLCTPILLPVVTALGVHPIHFAAIIGTNLGMGNVTPPTAPTLYFGGRMAKTP
                     VSQMMKPALVFITFAWLPTLLAVTYLPELALWLPRLVLGGRI"
     gene            34039..35247
                     /locus_tag="P5541_02215"
     CDS             34039..35247
                     /locus_tag="P5541_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRV70819.1"
                     /translation="MGKMDYLQEKPIAVLGGGATARGHAACAALAGREVRLYELPDFF
                     EGLGCIKENREIRLSGIQESLYGFKREGLAKIDVVTSDMEEAVKGAGIIVVSFPAVGY
                     KAFLEKLIPRLEDGMVVHFTTANFGSLIMRKMMRESGCSKRIVIGEWSSQPYGIRIKS
                     AGGQQMPEVSVNYWAISLRGSALPMIDQEKFFESKQYLPSLDSVVHPVRGDTVADIGF
                     SNVNPILHCPGTILGVGTMENWGVIYGGDKHDFSIYSHAYCPSISKVQLALYKEECAI
                     AEAMGVGIQQFSEESFFSRSNILGSEHMGGKFKVPFDEQYKLALGTGPFSIYNRYITE
                     DIPVGCHIFRELGKKFGVKVPVIESMITLASVMTGVDYWSEGVTLNDLGIEHMDREAL
                     NAYLREGTYL"
     gene            35331..36545
                     /locus_tag="P5541_02220"
     CDS             35331..36545
                     /locus_tag="P5541_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV70820.1"
                     /translation="MEWQGLFNGSATNRLVDSAIASIMKIASEENAMSLAAGEPFGAL
                     YPAEELRDCLMISFSKELPVWGYSNSRTGIAPLRQWIADWMARDGLLPQWANSDSLFI
                     TNGSQEGLYLLCECLLEPGDTVIVESPSYPEAFGIFRKFGAKCLSVPLSEEGPDIGVL
                     EGILKENRVKFFYTIPTYQNPTGFSTPDSKKQSVLDLALRHGFVIIEDDPYRNLSFEG
                     RPEGTYIRAAGDKKNVAYLGSFSKIVAPGLRCGWVVAPQPVIEKMLKIRIMGTLCLPE
                     ILHYGLWEFVNSIDLTEYLEGLSRTYRQHRDALVSSLMRHAGPHGLELAVPKGGFFLW
                     GRVPWIEDMKSFALYAIREEKIAVIPGKEFFLGGHVEEDTVRLSFSKVTPSMAEECAR
                     RLGTALKRFRDL"
     gene            36589..38244
                     /locus_tag="P5541_02225"
     CDS             36589..38244
                     /locus_tag="P5541_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV70821.1"
                     /translation="MGDHSKRELYGLLLNLVGIPSVSPSAESENRIARFIHDSFQDLS
                     YFQENRGDLRLLPLEGDPLGRHLVFAIVRARKRTADTVILMGHMDVVGTEACGFLAPL
                     AFSPEEYTERIASVAIPRDARKDLESGEWLFGRGVADMKSGVAAGMDLLMEAAENPGK
                     YDANVAVLFVPDEENNSGGMLSGSSCLARFQKEESLRYLACIDLEPTFATGDDARPTI
                     YLGSIGKINPFFFCTGKETHVGEYYEGFCAAPVISRINLMLDGNPEYADSLFGKSYPP
                     FGCMRQMDLRREYSATIMTKAFAFYSYLTSTRLPGQILSEMRGIAMEALEASIEKYRE
                     NTSSFAAMGGTTSKPRDWEPTVVSFDELSDMAEKVLGDGYARFMDETVGSAPEGSDER
                     VRAALLVESMVELCGIPGPMAVIGFLPPWYPHRSNLGETRGEKVVADAASATAGEMER
                     RFGLPLDIRPFFEGVSDLSYCGFQGDSSEMEVFARNMPGWGRPYRIPVEALAELDIPI
                     LNLGPVGKDAHKNTERIHLPFMLEVFPNILRFVVERIIEGYRS"
     gene            38361..39260
                     /locus_tag="P5541_02230"
     CDS             38361..39260
                     /locus_tag="P5541_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRV70822.1"
                     /translation="MTLWLKRILGRVPELRPSPGVLKEWGMIHLGCLLVAAGITWFLV
                     PNNIAAGGVSGLAMVLNRFLPGVPVGVLMMGMNVVLFVIGFIFIGFGFGAKTIYSSLF
                     LSGAVWLLERFYPVSGPLTEDIMVQLVFGTILGATGMAIVFNQNASTGGTDITAKILN
                     KYLNIDIGKGVLLCDFFIVLAAGWVFGIRTGMYAMLGVILNGFLVDSVIVGLRINKYV
                     VVISRQSEAIRRFIVEELESGATIYEGRGGWSGEPKEVITTIVNRRKFIRLRNFIKTV
                     DPRAFISVNNIHEVLGEGFERLA"
     gene            39309..40064
                     /locus_tag="P5541_02235"
     CDS             39309..40064
                     /locus_tag="P5541_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRV70823.1"
                     /translation="MRLEDRPVRLVLYNTRYGTGTGWAYHAPFPFAGTLKTTRERSSS
                     IARFIASLEPDIVGLVETDGGSFRHGGRSQPEYLAETLGHSWVFSSKYCIPRISGCVP
                     VFRHQGNAVVTSLPIRETSVRRLRRGIKNAVIETGFDDFDLKLVHLSLGSGARRQQIL
                     ELAAMARERKRPLLIAGDFNAFGGGPEIEPLIEAGLMNADTLGIRTYPSGRPRLGLDL
                     VLHSPEIDVRRIEVPDVRLSDHLPVVCDFALSL"
BASE COUNT         9023 a        10729 c        11444 g         9009 t
ORIGIN      
        1 ggtcagcggg aacgatcttg acaagtcggt ccttttcgat atcttccacg gatcccttag
       61 gcaagccccc atgataaggc agtgtccggt cacaatggag tgcagggttg tcgagacact
      121 ggaacttccg gtggacatcc tctttatcgg ggaggtcgct ggagtgtggt ccgaagagag
      181 gttcctcacc ggagggagcc ctgatataga gaaagtaaaa cctttctgcc tgacaatgcc
      241 tgataaccgt tactggtcca ttggtggaac tgtcggaagg gcatggagtg acggtgaggg
      301 actgaaggga aagttgccga aggtcggagg cggctcctga attgaactgg ctttcctaca
      361 caagcctggc atggaccgag aggatcctgg caccccctgg agatctggcc gaagaggcac
      421 agatctatat cgaagccgta aagaggaatt ccggcgggaa agccaggacg atgctgcacc
      481 tcggctgcgg tgccggaggg catgactttc atttcaaggg gcattttgag atcacagggg
      541 tggacctttg cggagaacag cttgcactgg cgagggatct gaaccccggg gttcgctata
      601 ttcagggcga tatgcgaaat atcaggctgg atcgaaagtt cgacgccgtc gtgatccccg
      661 actcgataat gtacatgtcc accgtggagg acctggacaa ggcgcttttg acttccgcaa
      721 cgcatcttgt acccgacgga gtctttctcg cagtggtcca cccgaaagag cttttccgta
      781 acaacaattt tgtctactcg gggtcggatg gggatacaca ggttactgtt tttgagaacg
      841 atcacgtggt ctcggacagc acctacgagg ccgcaattgt cttcctcatc cgccgcgaaa
      901 cagagctgca gatctataac gaagtccaca ctctgggact tttcagcaac tcagagtgga
      961 tgcgagcctt tgaaggatcg gggctggtcc tgaatgaaga gatcgccctg aatgatctgt
     1021 acgatcattt cctggcggaa gaaggtgagt acaggcttaa ggcttttgtc tgcgagaagc
     1081 gaaggcagtg atccggacag cgggacctga tcctgttgcg tggatctttc cagtaattct
     1141 ggaaagatcc agtatcctgt gtccattctt ttttccccgt attattggac aactctgatc
     1201 caattgagat agtatcaata agaaaaatga attgttttca gaaatttggg aaatatcttt
     1261 aaaggaggga gcaggaaatg ccgaagctgg aaaaggtaga aggcataggt ggaagctatg
     1321 cggttaagct ccagaatgcc ggtgttgcca cgaccgatgc gcttctgaaa atgggatcta
     1381 ccccgaaagg acgcaaggag ttagctgaaa gatcagggat cagcgagaag atgatactga
     1441 agtgggtcaa ccaggtggat ctttttcgta tcaagggcgt aggcgaggag tatggagatc
     1501 ttctggaggc atcaggagtt gacaccgttc ctgaactggc ccagagaaac cctgagaatc
     1561 tgcatttgca gatgaacaag gttaacgagg ccaaaaagct tgtaaggcag cttccctcgg
     1621 tgaaacaggt ggccgggtgg gtcgagcagg ccaaaaagct ccccagggtt atcaactact
     1681 gatagattta ccttaatgac atattcaaga gcccccttga agggggctct tgcctttcag
     1741 gtagaactgg ccagccaatt ttattgcatc ttttatttaa agtgatagag atgcagtatg
     1801 aagtataatt tgaaaggaaa atattttaac gaacaagggt tctctggagg gtatccgatg
     1861 caagacatca aaaccagctg gatacatgcc tgaaatggtc gggaagaatg gatagttcga
     1921 tacttctggt tcttgccgtg cttgccgtca cagtgttttt tcttgtcgtc gatgttgtca
     1981 ggatcgacgt cgtggctatt gtgtgcatgc ttgcccttgg atggacaggg gtcctttctc
     2041 cgaaggaggt cttcagcggg ttttccagca atgctgtaat ggccatgatg ggggtcatga
     2101 tcctgggcca gggtatagcg ggaacgggca tcatggacct gttctctgcg gcagtgataa
     2161 gaaaggcggg aacagacagg aaaaaaataa ttggactgat gtccctttct gtgggccttc
     2221 tctcgggatt tgttcagaac ataggggcgg cggctctttt cctgccgggg atactcaata
     2281 tctcccgccg ggaaaagatc ccgccatcag ccctgataat gcccataggt tttgcggcta
     2341 tcctgggcgg taccctgacc atgataggtt cgggtcccct tatactcatc aacgacctga
     2401 tccgcgactt cggtcttgaa ccctacactt tcttcagtgt cacccccgtg ggggtgctcc
     2461 ttctgatagc gggcacaagc tattttctgg tcttcgggaa atatattctt ccaggccagg
     2521 attcagggga ggtaccccta tccgtacagg acaggatcat cgaggcattc catctccctc
     2581 atcatatcgg gcactaccgc attcccggag gaagcaccct tgccagtacc accacggagg
     2641 agtcgggtgt gtgggacagg tacagagtca atgttctggg tatatccgaa ggcagggaag
     2701 ccgagtatgc cccatggcgg gagaccattt tccaggtcgg acaggaactg gccctccttg
     2761 gaaaagaaga agacgtggcg gcctttgcct ctgatcacgg actgataccc gtagatgaac
     2821 ccggcaggtt caaaggcctc aatgatccag taagggcagg ttttgccgag gtgatcatcc
     2881 ctccgcggtc ggagatgttg ggaaagtcta tccgccagtt ctccctcagg aagcgctatg
     2941 ccgttgagcc cgtaatgctc ttcagcaagg gggaggagat aaggggggat ctttcagacc
     3001 accagatcct tcccggtgac atcctcatag ttcacggcct ttgggagaag atctccgccc
     3061 ttaagaacga aacggatttt gtcgtcgcaa ctcccttcgt tgcagagacg aaggtccgca
     3121 cccggacatg ggctgcgctt gcctgtttcc tgggggccat aaccctggtg ctgacggggg
     3181 ccccgatatc gctggctttt ttcaccggtg ccgcggccat ggttcttgtc ggggttcttt
     3241 cgatccagga agcgtacaat gccatcgagt ggaaggttgt ttttctcctg ggcgggctca
     3301 ttcccctcgg tacggcaatg cagaaaacgg gcgcggccgc attcatggcg gaaaaactga
     3361 tggctcttgt gcaggggggg catccgttgc tgatggtcct tacagtatcc ctgctctcga
     3421 ccgttttctc tctgctgatg tcaaacgtgg gggcaatcat ggtcctggct ccgctggtca
     3481 tgaacatggg ttttatggtg gggatggatc cccgccccct tgcccttctg gcggcggttt
     3541 gcgcggccaa ttcattcctt ctcccgacac accaggtcaa cgccttcctg atgtcgtccg
     3601 gggggtatcg taacgcagat tacctgaaag cagggggagg aatgacgctg atctttttgc
     3661 ttgtagcagt tgcagcgttc cagatcttct acttctaggg cgggaaaacg ggaaacgaaa
     3721 acatttcagc aaccggaatg atttccggcc tgcaatttct cgagaggagg aatgatcatg
     3781 cttctgaaac atttctttac aggaaagata gctcacagct cgtacatact ggcggggaag
     3841 aacacctgcg ccgtgatcga cccgcagagg gatgtggata tctatatcag ggaagcccgg
     3901 tccatggggg tggatattac ccatatcctg cagacccatc ttcacgccga cttcatctct
     3961 ggtcacatgg atctggccag ggcgacagga gcctctattt acgtggcaaa gtctgccagg
     4021 tgcacctttg accacatttc cctgtccgag ggggacaccg tagagataga ggatatggca
     4081 ctgcaggtgc tggagacacc cggacacacc ccggaacatc tttcctacgt agtggttgac
     4141 aggtcccgct cagatagtcc tgcgggagtg ttcgtggggg acaccctctt cgttggagac
     4201 gtaggcaggc ctgacctttt ccccgacatg gcccaggaac tggcgggcga gctttaccac
     4261 agcctccaca ataaattgat ggaattgccc gattacgttg aggtataccc cgcccacggt
     4321 gcaggatccc tctgtggcag ggcaatgggc gccaaatgga caagcaccat cggctatgaa
     4381 cgaagattca accctgccct gcagattggg gacaggaaag agttcatcag gtcccttacg
     4441 gaggacatgc ctcccgctcc ggaccatttc agccggtgca gcgatatcaa tcgtcacggg
     4501 cctgccctgg tctcagacct tccggttatg gaggaactgg acccgaaaca gttcctggag
     4561 aagatgaacg atcccggcac ggtcctgcta gatgccagga gttattttgc ttatgccggc
     4621 cagcatctca agggaacatg gcatctggac ctcaacggca acttccccac tttcgcaggt
     4681 tgggtaatgc cactggaaaa tgatatcctg ctcgtcgccg acgattacaa aagtgcccag
     4741 atggccaata catgggcccg aagggtcggc gtcgacagga tagtagggta tctcgacggt
     4801 ggaatggcca actggaccgc tggggggctt ccctccagtc atatagaggt catttcggcc
     4861 ggggatctgc atgaaatgat cgacgaaaat gcaagcttca ttcttctgga cgtccgggct
     4921 ccccttgaat ttgaggacag ccatattgag ggtgctgtga acatccaggt ctcagacctc
     4981 agaacacgtt acggtgagat cgaccgggaa aaaccagtgg tggtaatgtg cagttcggga
     5041 aaccgctcca gcctgggagc cagcattctg aaacagcacg gttttgaacg cgtcatgaac
     5101 cttgcggggg gtatgtcagg gtacagcgca gccgggtacg tcagggaatg ccgggcctgt
     5161 atgaaccccc acggctcaag gttcttcacg acattcaccg aagtgaagaa acactgggac
     5221 agatcctgag caaaggagga cgggagctgt gaattacatg acggaagtcc gctggtcccc
     5281 ttatgttgca ggggtgggga taggtatact gagctggctc agttttctga tctcccgtaa
     5341 accgattgcc tgttccacca cctttgccaa ggccagcggg atggtggaga aactggtccg
     5401 cggggatatg gtagacagaa aaccctatta ccagaaggag aaacttgtcg ttgactggca
     5461 atggatgctt gtgactggga tagttattgg atccctcgtt tccgcgcttc tttcagggga
     5521 cttccactgg cagtgggttc ctgacctttg ggcttcaaga ttcggaacgg gtcctgtgct
     5581 gagacttctc gctgccgtgc cgggagggat atgcctggga ttcggggcca ggtgggccga
     5641 cggctgcacc agcggccacg gcatcagcgg gacgatgcag ctggccctgt cgagctggat
     5701 ctcgaccgcg tgctttttca tcggaggtat cgtcgtggcc ggggcgatct attgatcgta
     5761 cacacggggg gatgagatca tgacttcagg gtcggtgaac aaacctaaaa aggatatccc
     5821 gcagctcctc tggggtctgg ctttcggtat cgcgttcggt ttcctgctcc agaagggcgg
     5881 ggtgacaaaa tacgatatta tcgcagggca gcttctgcta aaggatttta ccgtggtaaa
     5941 ggtgatgctt tcagccatac tgacaggtat ggtggggatc tatttcatga aaagccgcgg
     6001 atgggtggaa ttatacccaa aatcgggctc cctgggtatg aacgtgatcg gcggtctgat
     6061 cttcggggct gggttcgcca tcctgggtta ctgccctggt accatcgcag gagccatagg
     6121 caacggctac ctggacgccc tggtgggggg gcttgcgggc ataattacag gggcggggat
     6181 attcgcggcc ctgtacccga ggctgaatga tggcatcctg aagtggggat actacggaaa
     6241 agtaacgctg ccgcaggtct tcaaggtcag cgaccgtgcg gtggttatcc cggtagtggt
     6301 tatcatcgtt ctgacccttt tcctggtgga gtttgcgggc ctatgaagtg ttcgtgtctc
     6361 tgctatcatg gtaagggtcc cattcaggag gtgacaattt gaatactctg gtttccttgc
     6421 ttgcaggttt cctgcttgcc gtgcttcttt atgtctcctt caaaataatc cgtcgtcccg
     6481 gtagttccag aggccgtgag accacgatcc actctagtat ccagcagctg aagtccatag
     6541 ggcaactttc tgtcttcaag gtgctcacca aggagatagt gaccgaggtg gatcacagct
     6601 ggggtgagat tgggaagaaa tacttttcct ggatactgtc gaacaggaag atggcaatga
     6661 tcttcgagtt tgttatcgat ttccgatacg acctgaggag ccccgacttc cagataatcg
     6721 aggagtcgga gggaagttac cttctccgga tgccttcctg tgtctatgag accagcatca
     6781 gggatatcca gttctacgac gagcagaagt gcaagctgct tccccttctt cttcctgacc
     6841 tcctcaatac gctgctgggc gacggcttca gtgaagagga caagaaccgg ctgaaggatg
     6901 cggcgaagag ggctgcagag tcccaggcag agtcaatgat agcaggtctc tgccccgacg
     6961 tggaacagtc ggccaaacag acgctccagt ccctggcaaa ggctttcggt gccgagaacg
     7021 tccggttcga gttcccccac gccggggaga aaggtgccac aagggacgaa agagagtgac
     7081 ggctgcctac tgatccaggc ttttcaccac ttcccttgcc caggcccttg tttcttccca
     7141 gtcgcggaag tcccctacgg ggcttttcac cattttgatc gctgccttct caagaagaga
     7201 aagcttgccc gggtcgatcc tgcccgcaaa gtaccccaga tgggccggcc tcaggggagc
     7261 tgttgaatca tccagggctt tccttcctgc tgcgatctgc tcctcatcgt caactgcgaa
     7321 agccatgccg acggcaaaag cccctgtaag acggtcccgg agggcctctt catacttctc
     7381 cacgaaggct ttgacctccc ggatctttcc atagtataca ggaccaccaa ggatcacgtg
     7441 gttgaactcc tccgggaggt gaacatcatt caggttcacc gcttcagcct cccagccggc
     7501 ggaccttatc tcagaagcta tcgtctcggc cacctcaccg gtcgaaccct tgctggtcga
     7561 gaatgccacc aggaccctgc ctttcgcctg ggtagaagat ccgactacct cgaccgcaaa
     7621 agcgcagcca gcgaagaacc aggccgtaga cgccataagg caaagggcga tgaagtcgaa
     7681 agctctagac agtcttttcg gcaatgcttg tccttctttc tccgtctgca attgatcact
     7741 cagcctggtg cagatggacg tcctgctgag ggaagggtat tgagatcccc tctgcgtcga
     7801 aacgcttctt gatcttctcc tggaggtcga agaagacgcc ccagtagtcc gaactcctga
     7861 cccaggggcg aacggcaaag tcaacgctgc tgtcagccag ttccagcact ccgatggtcg
     7921 gggcagggtc ctttaggaca cgttcatcct catccagtat gatctccagc accttcctga
     7981 ccttgtcgat atcgtcagaa tagctcactc ctgccacaag atcgacacga cggatctctc
     8041 tggcggaata gttgacgata ttgtcggcca tgatcttccc gttgggcaca atgatcaccc
     8101 tgttgtcggg cgatctcagg acggtggtga agatgctgac ctcctcaacg gtccctgcta
     8161 caccaccgcc ctcaacatag tcccctgctt tgaagggttt gaagaggatt ataagtaccc
     8221 ctgacgcaaa gtttgccagc gaaccctgaa gggccagacc tacagcgaaa gcggcagctc
     8281 ccactatggc tacgaaggat gcagtctgga cgcctatctg cgcaagggcg gcaacgataa
     8341 cgaaggccat tatggagaag tatgagagag tggcaatgaa cttcaccagc gttacgtcca
     8401 cattccattt tgaaagggcc ttcctgatca atgaacgtat ccccttggcg acaatgcggc
     8461 ccacaacaag tatcagtagc gcggctacca gtttcagcgc gtacagagtg atccagtcga
     8521 gcacgaccga tgatgttagt tccatgagag tacctccgtt cccttgattt ggatcaatga
     8581 ctatacctta cttgaaaata tcacctatgt ccctgtgagg gttatcgaat tcaaaaactg
     8641 cagggcggag aaggtgctag aactccaaca gttctaccct cctgttattg gctcttcctc
     8701 cctctgtcct gttggaagcg acgggcgcca gcattcccag acctgcgggg atcatcctcg
     8761 ttgcagggat ctggaacttg tccttgagga acttcacaac cgattcagct ctcctgctgg
     8821 aaagcccatg ttgtactccc tgtcgccggt gttgtcggtg tgccccacca ccaggaccct
     8881 cagctccggc aatgccttca tgagtttggc gatctcctcc agagtcggca ctgactctgg
     8941 ttttatgtct gtcttgtcgg tatcgaagaa tatcccgtag agtgctatcc ttcctgattc
     9001 ctcgatccct ttcgccatct cctctgaggt gactgtcacc atcctggtct ccattataat
     9061 aggatggcga tcagcgaacg gggggcaaca ttcttaggaa agtttttcgt tcctgacctg
     9121 atccagtcac cggggtctcc atttgtagct accggatgtt cgaaccactc ctggaggtga
     9181 tgtcttgaaa gtaattgaag caggggtctt cgtacaggcc agctccgaaa aggtttggag
     9241 agtactctca gatctgggga agtaccccga gtggaatccg ttcattctga tgatatccgg
     9301 tgagctggaa gagggggcca ggctttcggt gaagatcgga ctgccggagg ggagacccat
     9361 gaccttttct cccgtggtca gggtcgcaaa acctggagtg gaactgcgat ggcttggtca
     9421 tctgtggttc ccgggtcttt ttgacggaga acactccttc aggatagatg aggccgggaa
     9481 ggggcatgtc catctgatgc agagcgagca tttccgcggg atcctggtcc cattccttcc
     9541 cgcttctttc tttgaaagga ccaggcaagg cttcgaggag atgaaccgtg ccctgaagga
     9601 gagggtcgag agttccctct aatgaaagcc ctgtgtcgac aggaagcctg cgaccaggaa
     9661 tccgacacag gcgaggcctg ctgctatcat cgcgaaaggt agctgggtca gggcgtggcg
     9721 gatgttgtca caccccgttg aggctgacac aaggatcgtg ccgtcagagt agaaacagca
     9781 gtgttggccc agggtccctg cggcaacggt cgctgccacg gccaggacca gatccgctcc
     9841 cagagcctgt gcgagaggga tcaccagcgg gaatgcgatt aggtacagac tccagcttat
     9901 tcccacaagg aatgcgacga aaccaaggat aacgaagatg accaagggga agagggctgg
     9961 agagagaaga ggtgtagcct tcccgatgat gaaagggatc agccccagtt cctggttaac
    10021 gtaattgaag gtgaaggcca gacatgtgaa aaccattggg aacagcatgt tgccgatccc
    10081 cctcaggcag gtctccgtga aacgggcaac gggcatgatg ccctggatac ggtaaaaggc
    10141 gcaacagaag gatagtgcaa tgacccctcc cttgaaaagg ttgacaccga accacatggt
    10201 ccccgctatc agcacgagga ccggcagtac cagattgcgg gccatcgcct tcggcccttc
    10261 ttcactcata tcatgtggtt ttctcatcga gataagctcc gaacctggag gagccagttc
    10321 ccccgttctc cttacccttt cttcggcctg tctcatgggc ccgatgacgg gaacaatacc
    10381 tgtcaccacc aggggcacca tgaaaaaggc gaaccatgca tagaaattga aggggatcgt
    10441 ccttacgaac agggccagac cttcgccggc gggaagggcc aggtactttt caagcagcgc
    10501 ggcgatgaac accgcccagg cagaaattgg caccagcgaa gccacaggag cggcggagga
    10561 attgaccacg taggcggtca tctctcttgg gaccttgtag cggtctgcag aaggggtcat
    10621 gcagatcccg agggtcagac tgataaggaa atcatcgatg aatatgaaga ggctcatgag
    10681 ccaggtcccc aggagaaccc cccgtcgggt ggagaccctg gaagagaccc agtcgccgaa
    10741 acctttggtg ccccctgagt ggtcgaggac agcgacaaag gcccccatca ctgaacagag
    10801 gagtatcacg aagccgatgg tagggtcctg cattacacta tagacggtgt ccagcatgga
    10861 cgggaagaag gatagaccgt cctttagcat gaaccccaca gctatggcga tcaacaggga
    10921 ttccagggcg cgtctcgtaa cgatcacggc cacaagcaga acagtgactg gcagcaggga
    10981 tacccaacct tcaggattca tatcttcggc ccccaatacc gggaagaaag aattcttggt
    11041 caaacctgga cgatcataca ccctgccagg aagtggcgaa gcccctttaa tcatagtaaa
    11101 atagcattag ttcaaagaaa cacgaatatc gggaggagac cccaatggga acagatgctc
    11161 gctcccgtct gacagtcctt cacgttaccc ctgagatgac acccgtctcc aaggtgggtg
    11221 gccttgccga tgtggcggga tcccttccgg gcgcactcca aaggcttgga gtggacgccc
    11281 gtgtgttcac accatcgtgg ccgggggtgg ctgaaagact gagggatctt ggagccaggg
    11341 cgatatcagg acctgaccgg atatgtgtac cctttggtgg ccgaaaagtc tgcagcgatt
    11401 tcagtgtcgt cgatctggcg gggacatccc tctgcctgct ggaagatgat gaattgttct
    11461 ccaacccctc gatctacccg gaaaaactcg atcctctatc ggcaatgccc tttgccttcc
    11521 tctctcttgc cgcagtggag tactccctat cgaatgagtg gaagccccag attctgcacc
    11581 ttcatgactg gccatcctcc cttgtccccg cagcccttaa atggcacagg gtttacggcg
    11641 tcgcccgtga gggaccccgg accgtcctga ctatccataa catagcccac cagggcatcc
    11701 tccccctttc tgccctggat gagtggggtt ttggccctga ggcactcact ctggacgggg
    11761 tagagtttta cggaagcgcc aatctgctga agggtggcat aaccgcttct gatgctgtgg
    11821 tgacggtaag ccctaattac gccagggaga tcctttcgcc tgagttcggc gaggggctgg
    11881 aaggggtcct ggaatacagg aaaagcaaga taacagggat actcaacggt atcgacacgg
    11941 cgtcctggga tccctggaga gacaaggcga tacctgccaa tttccgcaca ggggacatga
    12001 agggcaagca aacctgtcgc gccgttctgt tatctgagtg cggatgggtg ggcttcagcg
    12061 gcccccttat cgtatcggta ggaagacttg tcaggcagaa gggcctggaa ctgctccttc
    12121 atgcccttga tgagatcctg tccatgggcg ccaggctggt gctgataggc accggagaga
    12181 gagaactgga ggagaggttc tccagggaag ccatccggag acaggatggg ctccatttcc
    12241 gtcgggcctt tgacgaaggt tttgctcgtc tggcttacgc cggcggtgac atcttcataa
    12301 tgccgtcact cttcgaaccc tgcggactct cccagatgat atccatgaga tacggcaccg
    12361 ttcctgtcgc cagaggtgtc ggaggtcttg tcgacacagt ctcggatgca gatgcggggc
    12421 cccgggggac gggctttctt ttcgacgatt tcactccaga agccttgatg tgtgctgtga
    12481 agagggcact aactcatttt tcagagcccc ggagctggtc ccagcttcgg aaaaggtgca
    12541 tggagaaaga cttctcctgg gacctttcgg cgagatccta catgacgctg tacagttcac
    12601 tgtcagatca atagcctttt tcaagactgg aggagctaga catgatatac gggaagcacg
    12661 gcagggttct tgccattatt ctcgccgggg gcaaagggga gcggttgatg ccactcacca
    12721 agtatcgggc caaaccggct gtcccctttg ctgccaagta ccggatcgtc gattttgcgc
    12781 tctccaacct ggtgaacagt ggtctctttt ctatctatgt cctcgtccag ttcaagagcc
    12841 agtccctgaa cgagcatata gaaaggggtt ggcagttcgg tggagccttg agggacaggg
    12901 atcacttcgt gaccctggcc ccggcccaga tgtggagagg tgagcactgg taccagggga
    12961 cgtgtgacgc tgttttccag aaccttcacc tggttactct ctatgatgcc gaccatgtct
    13021 gcattttcgc ggccgaccat atctacaaga tggacgtgga gcagatgctg gactaccaca
    13081 tggccagaaa ggccaacgtc accgttgccg ccaatgttgt cccccgttcc gatgcctggc
    13141 agttcggctg catccacact gacgaggctg ggaggatcat cgatttcgtc gagaaaccga
    13201 agaccccccc cgagatacca ggaaaaccgg gatttaccta tgtttccatg ggcaactaca
    13261 tattcgagcg ggaaaccttg gaggaagcag tcatcgacga tgcccagaac gaggccagca
    13321 gtcacgactt cgggagggat gtattgccga cgctgcacaa aaagtgcaat gtctttgcct
    13381 atgacttctc caccaacgtc ctgccgggca aggacaggcc ctactggaag gacgtgggca
    13441 ccataaaggc ttactgggaa gcccacatgg acctttgcaa gcacgactcg gacatgaccc
    13501 tattcaaccc aaagtggccc ataaggaccg tttcattttc tgatcccccg acctactcct
    13561 accctgtggg ggggcagatg tgttccataa taggctccct ccgggccgag gggagcaggg
    13621 tgctcggcgc catcgtacat agctcggtgc tctcccggaa cacattgatc aattcggggg
    13681 ccattatcga ggacagcata ctggggcaag gggtcgagat cggagagaat acaagactga
    13741 agaaggttat cgtggacgcc cacaacagga tccctcccaa cagcgttatt ggctacgatc
    13801 tggaagagga caggaagaag taccacgtcg acgaggagtc cggagtggtt gttataggaa
    13861 tgcctgccat gcagcttagg aagaatatag agatcccttt catcccaccc ctcgaaaggt
    13921 aggtctttaa ggaggtactg gaatcagggg gaataaatca gtataatggt acccgatttc
    13981 tccaggttgt cccggaaatt actgaaaggc ggcatcagga ccgatggtga ccgacaggta
    14041 ttctgtcggg atgagatagg ctcctgaagg taatagttga tattgaggtg aggcacttga
    14101 ggaacatgat ccagatatcc gaggcatcat cgctggcctt ccacggaatg ggtctcctgg
    14161 cccggagcgg gggacgcctg agcgttcacg agatggcctc tctgacgggg tcatcggagg
    14221 cccacctctc gaaggtgttt cagagactct cccgcgccgg gttcgtaagc tccgtacgtg
    14281 gcccgggtgg agggttcgta ctctccagac ccgccgaaga gatcaccctg ttggacatct
    14341 atacagccat tgagggtgaa ccttccatgg acacctgctt cctggggagc ggcatgtgcc
    14401 cctttcccag gtgcatcttc ggtgaagtga tcccagatat catgaaacga ttaatggact
    14461 atttgtctgg taccaccctt gccgacgtcg tcagggacga ctcctgactg aggaaggctc
    14521 cccttttaga tctgcatggg tctcctctgg tcaaggttct ttttgtcgct ggtctacaag
    14581 aatcgtcttc ttcctattgc atttggcttt tttatccgct atatttccct gcaaagggag
    14641 tgtccgcaaa aaagatgaga agagtcgagg ttagcagagg ttccatgaag tccgcaaact
    14701 ttttctggtg gtggcgccct taggcgccgc agaccgttgc gtgatcgcac gcggggctgc
    14761 ggtataaaac cgcggccccg cgttttttgt atcgacgagg ggcgtatccg aaaggatgag
    14821 cgcccctttt tttgtggaca ccccgaatct ttgagccgag gaggaagagg aaatgaggag
    14881 catgaggaag gaagacgagg caagaaaggg ttatttcggg actttcgggg ggagctacgt
    14941 ccccgaacag ctgcagccca ggttggacga acttgatgct gcattcgtgt cggcaatgga
    15001 ggatgatctt ttcgccaagg aattcacgag actgcttaag gagtatgttg gaaggccatc
    15061 cgcactgaca tactgtgcca atatttcgag ggaatacggc gggggaaggc ttttcctcaa
    15121 gcgtgaggac ctgaaccaca caggggcgca caagatcaac aacaccatag gtcaggcgct
    15181 ccttgccaga cggatgggca aaaaggaact gatagccgag accggggcag gcatgcacgg
    15241 gacagccact gcgacagtag cagctctgat ggggatgaaa tgtaccgtct atatgggtga
    15301 ggtcgacgta cacagacagg ctcccaatgt atcccgaatg aaggcccttg gggcggaagt
    15361 ggtctcagtc acccatggcc agaaaacact caaggaggcc gtcgacgcag cccttgaagc
    15421 ctacgtgttg aacccggaga ccttctacct gctgggttcc gccgtaggtc cccacccctt
    15481 cccgacgatc gtacgtcagt ttcagagcgt tataggcaag gaagcaaggg agcagttcct
    15541 cgaaagggag ggtaaactcc ccgaccacgt tctggcctgt gtggggggag gcagcaacgc
    15601 catagggatc ttctccggct tcctcgatga ccggggggtg ggcatcatgg gtgtcgaacc
    15661 ggcgggcaga gggcttgaga cgaacgaaca cgccgcaacc cttgtagcag gcaaacccgg
    15721 cgtcatacat ggtttcagga cttatgtcct taccgataat gacggggagc cggccccggt
    15781 ctattcaata tcggcagggc ttgactatcc cggcgtgggt cccgagcacg catttctcat
    15841 ggagagcgga agagcttcct acgtctctgt aaccgatgat gaggcagtgg atgcattcag
    15901 gatcctatgc aggctggagg gtattatccc tgcccttgaa agctcccatg cggtggccca
    15961 ggctctgaag atcctccccg aactttccgg ggacaagacg gtgcttgtca acctgtcggg
    16021 aagaggggac aaggatcttg attctgttct gccgttgttg ggtttgcagg gtgaagtaag
    16081 ttaaatgaat cctgttcctg tcgggaggtt ttcccggaaa cgatccgcct ttaccggttt
    16141 cgagaagttt tggctggatc gaattttcca gggaaagccc ccggcagggc aggaaacaat
    16201 tttgcagatc cggatctgtc tttgtgcagg tcactctgaa gttgtttgct ccactggttc
    16261 aacaccgttg gagtaaaatc atcatgataa tatccagatg cagcgggagg acaagcagct
    16321 tccaatgaaa aacgacaggg caaggcaagc tgagagggtg tcatgggttg gtctgggggc
    16381 taacgctttt ctcgcgctct tgaagctatc ggctggcttt gcgggcaaca gcgcagccat
    16441 gatagccgac ggagtgaact ccctctctga ccttggcacc gatatcgtcg ctgtcgtaag
    16501 tttcaggatc acagggcgcc caatagaccg atcccacggt tacgggcacg ggaagttcga
    16561 gaccctcgcc acggcgataa taggaagcgc ccttctggcc gtcgcagtgg gcattttctt
    16621 cggtggcgga gagaggatcc tgaccgtcct gaggggcgga accctgcccc gcccgggttt
    16681 ggtggccctc gccgctgcag ctgtctctat cctcatcaag gagactctct atatcttcac
    16741 caggcgggcc gccctttccc tctccagcga tgcgcttatg gccaaggcat gggatcaccg
    16801 atcggatgcc ctcgcatcga tgggtgccat ggtcggtata ggaggtgcca tttttctggg
    16861 tccaaaagca agggtccttg acccccttgc cgccctggtc gttgcggcga tcattttgag
    16921 gatagccttc cccatcacag taaagagcct tagcgaactg cttgaagggt ccctctccag
    16981 ggaaaacgag gaagagatca tgcaggcaat atcggaagtc ccgggggtcg agggtgcaca
    17041 ccatctccgg accaggaagg tcgggtctga cgtggctgtg gaggttcaca tcatagtggc
    17101 gagggacctc ggcatcaccg aggcccacaa catagccacg gaggtcgaat ccgccatcag
    17161 ggcgctccac ggagaaggga cctttgtatc cgtgcacccc gagcctgagt cgggagacct
    17221 ccagggtcag gttcctttcg acgacggtca taatggcatg gaggatggcc cgggtcacca
    17281 cgagagggtt cccgatgatc tccacgaatg atgcctctca gaccgacctc cacttcagaa
    17341 aaggggtttt acagccgaga tccaggctcc tgcagcaacg tcttttgcgt caatgtcagc
    17401 taccccctgc atatcgggga acatgtcctg gagctgctcc ggagtgaatt cgtaaaccct
    17461 tgtaggctct accctgacat tttcgaaccc cgctgccttc agttttcctg caaattcttc
    17521 cattcccagc gccccggcga tgcatccagc ccatgcgtct atgctgctcc tgatctcttc
    17581 aggcaccggc ctcaggaata ccacatcaga aacggagatc ctgccgcccg gcttcagcac
    17641 cctgaagatc tcccggaaaa cagcatcctt gtcgggggac aaatttatca cgcaattaga
    17701 gactaccgca tccacgcttc cctccggaag ggggatctcc tcgatgaagc ccttcaggaa
    17761 ctccacgttg tcaaggcccc agtggaccct gttcgattca gccgcaagga tcatctcgtc
    17821 ggtcatatcc agcccgaaga cctttccgga aggacctacc tccccccccg ccaggaatgc
    17881 gtcgaggcct gccccgctcc ccaggtccag caccgcttcc cccggcttga gcccggccat
    17941 ctcaacgggg ctcccgcaac ccagggactg accaaggacg gcatcgggta tccggtcctt
    18001 tgaagttccc tgatagatgc cttcagtaat ggggtccgat cctgaagcgc agcaagatga
    18061 ccccgaaccg cagcaagatc tgttttttct tacggcttcg gcgtacttct ttcttacagt
    18121 ctcgcgtatg tccttcaaaa tgcgcatccc ccttccgttc gccccttcag gagcctttca
    18181 agatcaaaac agacacactc acctctctcc agggcctcga caacggatcc cagacccgag
    18241 aggaagacat tgcgatgatt ctccgggatc ctttcgatca atgaaaggaa gaaactgttc
    18301 attgaattct ccgtcccttc aaaaaaacgg agccctttct ccgttaacga aagtgttacg
    18361 gaccttcgat ctaccttgtc ctgtgacctg acaacaaatc cctgctccac aaggctgtcc
    18421 gtcaatcttg ttactgtgct cctgtcaagt ctcagggttg ttgccagaaa gcttggggtc
    18481 gctacctccg ctctccctat ctcgacaata gcatggcact gggcaagggt tacaccacag
    18541 catgatgccc tgtccctgtc aagaagggaa aggttccttg ccattgttcg gatcatctcc
    18601 cctaacgcct tcgcctgttc ctctttctcc agggtatcca cctcctgaac gcaaaagatt
    18661 gtaatatgca aaagttgaat aaatcaataa aaatataatt aaatcgtttt cttcttccct
    18721 tcatgcatgg tatgattttt aaagatcaag gttttgtttt ctggaaagaa cattccaaag
    18781 tcatgaaccg gaggggatag ttgtggtcag tgaaatgttc cgtatcagga aggagacact
    18841 ggacgagatc aactgtttcc tgctcgatcc aggcaatccc gacgtacagg ccattcatag
    18901 ggtagttgcc aagtacggta caccgacgga gatcaatgcg aaggctaagg aggcaaggtg
    18961 cctttcgaac ctcatgggac ggctggagcg aatgggatct ccttacctgg agaacctgaa
    19021 gtggcttgtg gcggagaggg acaggggtgc cttcattcct gtctcgcaat accggaagaa
    19081 gatccttggc gacgaagcca cgggaaagac cttcagagag tcgacttcag tgaccctcga
    19141 gatcagtgcc ctgcagtact tccccttcct gatcgcagag gcggaacagg ccatcgacgg
    19201 caaggagatc atgccaggga gatacataag ggtccgcaag atgaaggagc aggagagcga
    19261 ccagggcgat atccttgccg tggcggcggc catgcagatc gtcggagcca gctacgtgga
    19321 gaccctcgac accaagggga ccgacggctc caatattcat ctaggtggcc ccgagaccat
    19381 cacgggatac ttcggaggca ttggtcagcc caacgaccat gccctggcat gggtcgacga
    19441 gctccttcac tactacacga actacgggat caacgaggtt ctgaacatca accccgggac
    19501 ggtactcctg ggttacctgc tctacaggct gggggtggat gtccgcttca agatatccgt
    19561 attcatgggc aacgacaacc cctacgcggt cctctggacg ctgatgatgg ccaaattgtt
    19621 cgccagggag gacgggtcga cgccgttgat cgggttcaac ctctccaact cggtggacgc
    19681 agaggctctt atggcctgtg ccgacatccg cagaagactt ggcctggagg acaaggtcag
    19741 gatagagcac cacattaccg agacctacag gagcatcgtc cgtcagccct acaacaggcg
    19801 cgaggatctt ctgaaagtcg cccgggtcta cccgaacatt tccgcaaagc acgagggagg
    19861 cgacccggag gtagaacaga ccatcgccca cccctctgac atactggact acttccgcga
    19921 caaggaagag gtcatcgcct ccggcgatat ggataacctg aagaacaact acctcaaaaa
    19981 gcacgatgct acgaacagaa ccgcagaaga attgacgagg gcggggctct cgtttctagc
    20041 cgcttctcgc ctccatgcac gctgatcatg atcctggttg cccgggaaga tcttcccggg
    20101 caacttttct atcggatgga ccatgactga aagcctcaaa gggataaatt attctgtaag
    20161 ggtaagcgcc agggcacgcc gtgtgacttt acggattgct ccccctgaag gacttgttgt
    20221 ggtaataccc cgtaacttca gcaagaagag gataccctcc ctgatcgatg agaagcgcca
    20281 atggatcgaa aagacccttt caaaggtggc gccccaggca gtgactgcaa gcgggacttt
    20341 gcctccgaaa gagtactttt tcccctccct gaagaagtct ttgggcctcg agggcccgaa
    20401 cgccgtttcc cgccttcgct ctatagctga ctcccatctg ctccctcggg caagggtttt
    20461 gtcgcttaag acaggactta ggcccgcttc cttcactttc aggaaccaga aaaggatatg
    20521 ggggagctgc aattcgaaga gaaaaatcag cctgaaccag aaactactct ttcttgaacc
    20581 tcccctggtg gactacatca tcctccacga gctctgccac cttgagtaca gagaccactc
    20641 gtcctcattc tggaccttcc tttcatcctt cgatccacac ttcagggatc accggagaga
    20701 gataaggtcc gcacagtgga cgatccccgt ctgggcctat tgacacagtt tcttattttg
    20761 gtaccataat acctaaaata aactgtgagg tgttcactat ggcggagaag gttttcagaa
    20821 gggtcatcca gatcgacgaa gacaaatgcg atggctgcgg ccagtgtgca gaagcctgtc
    20881 acgaaggggc catccagatc atagaaggca aggcaaagct tgttagcgac tcctactgcg
    20941 acgggttggg agactgcata ggggagtgcc cccaggaagc catatccttc attgagcgtg
    21001 aggcagagcc ctacgatgag gaggccgtca aggagaacat gggttcgaag aaagaacagg
    21061 agactctacc ctgcggatgc cctggcacca tggctagatc cctggaaggg atccttgaaa
    21121 ctcattgcga tgcttcaagc aagcctcagc tccgatcgga agaggctgtc tcctcggaac
    21181 tggctaactg gcccgtccag ctgagacttg tacctgagaa cgccccttat ctgaagggtg
    21241 cggatattct gttggcagcg gactgctcgg cagctgcttc tccggatttt catggccgtt
    21301 tcgtcagagg ccgcatcctt ctcatggggt gtcccaaact tgatgacgca gactactaca
    21361 gggaaaaact tgcgaggatc atcagggtca acgattcgcc ctcgataaca gtcgtcagga
    21421 tggaggtccc ctgctgcgga ggtcttgcac agcttgctga ggcggccatt gacgaggcaa
    21481 gggtggatgt tgaattgaga accgtgattc ttgattcccg tgggaaactg gttgaagacg
    21541 agacagtcaa gttccggttc cagtaattag gggtattgta aacccccata gacttgaggc
    21601 cagcaggcct acgggaagct gcaaggaaat tacccggtgt tcgccttact ggctgcaggc
    21661 cttacagcga actgtcctct atctcctgga tcttctcctc gaagccgtag atcgttccgt
    21721 cgatttcttc cacggtacgg tccaggtagc gcatccccgt gatctcgtac cccgggggga
    21781 gatggttgga gaagcgcttg accttgagta gcgacttgag gtagctcttc tcgaagagat
    21841 ttatcacctg ggcggccgta agggcatcgg cctttccgtt ctccagaaag agacaggcca
    21901 tctcgaggtt ctcgccgatc tccatctctc cataccagat gtcggccagg atctccctga
    21961 atcgttccct ccggtcgggg gtcaccatct cgcggataga tctctctatg aactttaggc
    22021 cccttctgag ctcaccctcc acgatacgtt catccccgtg gggcagtgag aagtcggcta
    22081 tgtactcagc ctcgtcactg atcgttctcc gtcccttcag cagtcttcca taatccggtc
    22141 tgagtgttac cgaggctctt tccggagggg ccgctgtcag ctgccggaag accttcccgg
    22201 tatcaaagcc cttggattcc tggggggaac ggttgagcca ggtcacgtaa cctgtttcga
    22261 agaggccttt acgaccggcc cttcccgcca tctgcagaaa ctcgttcctg gatatgggct
    22321 ccctgtcgtg gtacctgacg agctgggcaa agatgacgta ctctgcaggg aggttcaccc
    22381 ccagggcaag tgcgttcgta ccgcagacca cgtccaggat cctttccctg aaggcggact
    22441 ccaccaggag cttctccttc ggcaacatcc ctccgtggta gactccaacg cccctcagaa
    22501 gaggcatgtg gacatcccgt acatcaagga tctcggccag ttcatagagc cgctgcctgt
    22561 gggcctttcc cttccggggg cgtctgcggg ctatcttgta ggccaggtcc acagctcctt
    22621 tctgggagaa gcagaagacc agggcgtcat gaagtctttc aagggtggcg ggcttcttcg
    22681 gtgtgaatac cagttttgtc acacgttctt gcccctcgta gaccgtgaat ggtcttccgg
    22741 ttatctctcc gaggtaatcg ccgacagagc gggcgccccc gaaagtggcc gacatgacca
    22801 gtataggcga gtcggggttt gtggagacaa ttccctcgat ataggtccgg gcacggtcgg
    22861 ggtcgtcgaa gatgtagtgg aactcgtcga tcacgaccct caatcccggc gagccggcgt
    22921 acttgagggt gtagatctcc tgggtgcagc agatgaccgg tgcgttctcg ttcttcttga
    22981 agtcgcccgt ctctatgccc acatcaagcc ccatccgcct gagttcaagg tagcgctcgt
    23041 tgctcagggc ctttatgggg gcggtgaaga tgatccgcct cgtgtccggc ggccagatat
    23101 gaccatcaag gtccattagg cccgaccaca ggtaggccac ccaggtcttg cccgacccgg
    23161 ttggcgcggc caggacggcg tccctgttcc tgatcctttc ccaggccttt acctgccagg
    23221 ggtagaaact tccgtattgt tccgccatgt ccgcctcatt tcgtgaagtg ttgtcgcaag
    23281 tgtcccggcg ctacttatat tactatgaac gtccctttta cgaattcaat attgcctctt
    23341 ccttgacaag aggagaaatc cgataatatc atcggtttat gtcaccggtt ggtgacagga
    23401 ggtgccatgc gcgataaaga ggagaccagg aaaaagatcc ttgaagcggt gggcgagatc
    23461 ctttcagaag aggggttcgg aggcatcggg gtcaacaacg tatcccggag agccggcgtc
    23521 gacaaggtcc tgatctaccg ctacttcgga gatcttccag ggcttctgag ggagttcgct
    23581 tcagggggcg attactggcc ctcgatggag gatctggtcg aaaaggcgga aggggataac
    23641 cggcctcccg gagcttccga actcgccgtg gcccttctca agggctacat tcgggagcta
    23701 agggattcga catcagccca ggaactgctc cttggggaac tggtatcaag gaacgaactc
    23761 tccgaggcat cggcacggaa gagagagagg cagggagcgg accttataga ggccctggtg
    23821 aggtccgccc ctgaagcgga gagggtggat gttgccgccc tgggtgccct tctctcggcg
    23881 ggtctcacct ttctgatgct cagggcacgg acatcggagg cctacctggg gatagacatc
    23941 tcatccgaag aggggtgggg gcgcatcgaa agggctatgg aggagcttgt cagaggagta
    24001 atgggtgacg acaagctgga aggcttccaa tattagggct gttcccgtga ccatgcgaca
    24061 cctgacagga taaaaaagga ggagtgcttc ttgaaaagac ctgttcttat tcttatttcg
    24121 gcgatcatca tgacagccct tgggtgcgga acagccctgg gggccgggtt tgctctttac
    24181 gactggagtg ccagagggaa cgccctcggg ggcgccatga ccggtcgggc cgacgacccc
    24241 tccgccatag cctttaaccc ggcgggcatc acccagatcc cggactcgtc catgatggcg
    24301 gggctggctt tcatcatgcc gggagggacc ctgacggaga taggcggtga tagtgttggc
    24361 atgatcgacc acaccttcat ccctcctcac ttctactaca cgcgacagtt gagtgaccgc
    24421 ctctggttcg gcgtgggcct catgacgcgc ttcggtctcg gcaccgactt cccgcccgat
    24481 tggtacgggc gctacaacag ctactacgcc aacatccagt ccgtatccat caaccccaac
    24541 ctggcctgga aggtgaacga ccggttgtcc ctttccgtcg gggtcgaggc gctgtggttc
    24601 gagtacgggg cgagaagatt tattggaaca gacttcaggc tccttggaga cgacataggc
    24661 tacggctgga acgtgggact ccattacaaa ctggacgagg cgacccggat agggcttcac
    24721 tacaggagcc aggtaaaatt gaccatctcg ggaaccgccg acatacaggg tgtcggctct
    24781 acggatgcct ggggagatct aaccctgccc gagatgttcc tgttcggcct cagcaggcag
    24841 gtaactccta aacttaacgt cgaggtggga gccatataca cggggtggag ctcctatgac
    24901 aggttggcga tacatttcga gaacgatttc gggatgctcc tcaacccatc cacttcagtg
    24961 aagaactgga agaacgtctg gaggtaccag ctggggcttg agtacaggta cagccccgag
    25021 tggacctgga ggttcggcta cacttacgac caggagcctt cgccggacgg cacagtcgac
    25081 tttcaggctc ccatgagcga ccgacagctt ttctccatag ggttcggcta cacgaagaat
    25141 ggccgaacct gggatcttgc ctacacctat ttgctggccg acgacaggac catagctggc
    25201 cggcccggag acggaatacc caattcgcgc accagcaaca tggatgccca catcatcgtg
    25261 atcagctaca gcatgaggtt ttagaggcgt tacgttcaag gggaaaaagc gtaaaccgta
    25321 aacggtcgac ggtgaacgga aggatcaaag tcccgagggg attaaactcg ggaggttctt
    25381 taacgagggg taccccctgg cactcctcct gggagacctc ttaaacgaaa gcagtgaagc
    25441 agaaagagaa accaatgctc aaaggccgga ggatcgatct tcggcctttt ttgccgttca
    25501 ctgttcactg tttaccgttc acgcctttaa ggttttgcct tttcctgtaa aattacggaa
    25561 caggagatga ccgaaaggag ccgaaaacga tggagctcga tttcatcaag ggtcacatgg
    25621 gaggcaacac catcctcctc ttcaacggaa ggcaggtcag aagggaatct catgtgcttg
    25681 agacggccct caccgttctc gaccggaaga gactttacgc cgaccaggcc gcctttctct
    25741 acccctcggg aaaggccaac accatccggg ctctcgtggt ggatattacc aaccgtaact
    25801 tcatccctgc atgcggaggt ttcactcagg tcctgggaaa ggccctggtg gatacggacc
    25861 tggtaaagtg gttcaaggtg aagaccgctc cacaggaatt cgaggttacc cttgaactgg
    25921 agtcgggtcc ttccacggtg acggtccata agacaaaagg cggcaagttc agaaaggccg
    25981 aaagcgacat gacctccttt gcccggatgt tggccggcga cggggtcatg cctgtggacc
    26041 tcgacgggat ccaggccttc aagtccggct actaccttgt ccaggaagct ccgaaattga
    26101 aggaacacta ccccgaggcc gacttcgaag agatgaacac cgccgccgtg aaggccatat
    26161 ccgaggcgca ggccttcttc caccagaagg gttactcctc cacccttcat ttttcactct
    26221 ttgacagatc gcccgcccac aaggggcatc tgagggcggt cttcccccac ggggttctca
    26281 ctggtaacat cgagcccacc tgcgggacgg gcagtacagc ccttgccctg gcccttctgt
    26341 tgaccggcga gggaaaagat cttggcctgg ttaagggcgg aaggatcaag gtcagactgg
    26401 agaccggtgg aggccctgct cttggcggcc ccgacataac caccgtggtg gtggagaaaa
    26461 aaggtgaaga ggtaaccagg gcctttttct cccacagcct tgtagagata acggcaaaag
    26521 ggactgtatt cgtgtaattc aggccgtatt ttcctgaact tgcatccccc gggcctttgg
    26581 tgtttattat ctttcagtga ctagcctcgg agtattgaag ggtcaggatc ggatccttgc
    26641 ggcttggacc gtggccgctt ttttgttcat tggtatcgcg aagggaagag acttcaaatg
    26701 actagcgact ggaccagatc tcaacaaagt gttagcggtt ttgaacgggc catggcactg
    26761 gccggtgctg ccaccctgaa agaagctctg cctatctgcc tcgaggcgtt gataggatct
    26821 tcggaaggct ctcttgactg tggatgggtc tacctggtcc ccgagaaggc agaaatattc
    26881 aggctccgtt gctcaattgg ggtcagtgat cctttcgtgg aggccaacgc ttcctgggct
    26941 cccgattctg tacacgggag ggcgatcttc agggatactc catggtacgg gtccttttcc
    27001 gagaccctga acggccagga tccgctcctt gcctccgagg gtctccacgc tttcgcaatt
    27061 ctccccctct tccggggctc ctcagtagca ggttgtttct gtctcgcctc tcacgacagc
    27121 gacacagttc cacagcacgt aaaacgtgac cttgaggctc tctcccggtg gtttgacaag
    27181 accatagcca gggtccaggt cagcgagagc aggatcaggg agtcctggga gctgctatcc
    27241 cttttcgacg ccatggccga gatggtcttt gccttcgacc ttgacggaga gatagtctgg
    27301 attaacagaa ccgctcggga agagctcggt cttgataaca atgggaggat ccacctcctt
    27361 gaccttcatc ccgatgatac gaggcctgaa gcggccagga tctttcgcag gatgctctcc
    27421 cgggagcagc agacctccaa tgtgccctat gcaaaaagag acggaagtgt ggttcatgca
    27481 acgacccttg gcgttcccgg gagatggaag ggagaggacg ttctcttcgg cataagcagg
    27541 atccaccaga cgggggggcg taccagcgtt gaagctgccg cagaagtttc gagcctggag
    27601 agggatatcg ccaggatggc gatggtcctt gacgaaaggc tttcaaggat cgaggaggcg
    27661 cttatgatct caccggagag ttctgcaagc aggctgtgga cagggagcat aaaggaatga
    27721 acacgtcggg agaggggatc ctcttcacca agatgcacgg aaacggcaac gactttctga
    27781 tcatcgacaa caggttgggg gagatacctg gccatgaact cccggatctt gcgcttcgcc
    27841 tctgccgaag gaggacgtcc atcggtgggg acgggctaat ggccgttgaa aggagtgata
    27901 ggggcgatat ccgtatgcgc ctcttcaact gcgacggatc cgaaggggag atgtgcggca
    27961 acggcgccag gtgcctggcc cgttacgcct tcgagaaggg tatggctcca gccaggatga
    28021 ccatcgacac cctggcaggg cccgtagtgg cagaggtgga gggggtcttt gcaaccctcg
    28081 acatgggggt cctcgaaaca gcatcggcac tcaggggaca ggttctgata ctctccggcg
    28141 agaggttagt ctacagtcag cttaatgtcg gggttcccca tacggtgatc ttccaggacc
    28201 cgggggatga aagggctgaa atggatcttg ccaggatcgc cagggccttc cagtccgaca
    28261 gggagcgttt cccccaggga acgaacgtga acttcatgaa gatcaggacc accggggaga
    28321 tcgacctccg gacctacgaa cggggcgtca acgatttcac catgtcctgt ggtaccggct
    28381 caaccgcagg cgcagtcgct gcatggctgg agggcaaggt gtcaaccccg gtggaggtca
    28441 gaaaccccgg aggggtaaat actgtcctca tcgacgaaga gggtcccggc agggttcgtc
    28501 tcttcctcaa ggggcagacc acggccgtcg ccgagggggt cgtttccggg gagtgccttc
    28561 agatccgtga accgtgaatc gtgaaccgtg aaccgaaaaa aaagatcttg taccacatac
    28621 taagtaccct aagcgcacct ccagcgtagt ttagggctac agacgaagta ccccgaacga
    28681 agtgatgagg taggaacctt tttatgtaat cccgagtagt gcaacgacga ggaatctgaa
    28741 caaggggctg ttggatcagt tctccgccga taggcggacg tcccgcctcg tccgacagca
    28801 ggcggattcg ggatgacgat atctccagaa cttgcgacac ggtttacgat tcacgattaa
    28861 agggttttcc gagttacgtc tcacgattca cgctccctca cccaccccga tagcgactca
    28921 agcccccgtt tcagcgcatc ggttccatag gcgtatccga aacgcagcct cttgtcgatc
    28981 ccgaaacagc tacccgggag aagaaatgtc cccgtggtac caagcaggtc cacgcagaac
    29041 tcgtcggatg gtatatccag gtcgtagccc acaaaggcag ttgtaccagc cgttggctta
    29101 acccagtgga agagaggttc tgagtcgatc cattccccaa gcaggccgac gctctccctg
    29161 atgatcttaa ggttcctctc cgtgatcttg tcccggttct caagggcaac ggttgccagg
    29221 gcgtccacaa ggggtccgca actgatagtg gtgtagtccc tcctagaaaa ggcctcttcg
    29281 atgaactctc tgggtccggt catccatccg ctccttagac cggccagaga gaagaccttg
    29341 gacatgctgc ccgtgcttac gcctttttcg tagatgtcag ccattgacga gaccgaaaca
    29401 ccgggttcgt ggaccaggcc gcggtaggct tcgtcgcaga gtacccaggc cccgtgttta
    29461 cgtgctatca ctgctatcct ctccagcgtc tccctgtcca ttagggaccc tgtagggttg
    29521 ttggggttgt tcaggcagat caggtctacg ccgctttcag ccatcttctc tagttcatcg
    29581 gtatctggaa gccagaaatt ttcgggccgc agttgaagga tatccacttt tgccccgaaa
    29641 gcctttggga tgctggtgag ctgctggtag gtgggccaga cgctgatcac cctttctccg
    29701 ggacccacga tggtaaagag cgccaggaag ttggctgccg acccgccgtt cattatcaaa
    29761 actctctccg gatccctgtc ctcgtagagc tcccctatca ggccccggag cctctcgctc
    29821 ccctttatgt ccccgtaggt catccgggtg cccaggagat ctaccatgac ctcctccttg
    29881 cggcccgaca tctcaagcag ttcgtagagg agcagcgaat ccacgcaagt ctctgcgatg
    29941 ttgttgacgg caagtgtctc ccagcgattc atccactgct ctacgccaaa gggttctatc
    30001 ttcatcggtc gtcctcctgt aaggtcttat cacctatctt attctaacct gccaggaggg
    30061 ttcaaaacag aggagaatcc aattcactgc tttatctatg tgattgttct tggggttgat
    30121 ttgacagttc catgtctggc tggtataatc acttatgcaa aatttggaaa ccttttttcc
    30181 tattaggaaa aaggaggagg gaaatgagca gtcttgaaaa gtctctcatg atccttaaac
    30241 gccttggcga ttcgccatac gagctcaacc tttcagaact tgcctctcac gtcggaatgg
    30301 gtaaaagtgg tgccttcaag ctgataaatg tcatgaagaa acacaacttc gtaattcagg
    30361 atccttcctc gaagaaatat cacctgggcc ccataatgtt acgtctggga aacgtataca
    30421 gccgcttcaa gggaatagaa cagatcgcga agccccttct cttccgcatt tccaggtcat
    30481 tccaggagac gacctacttg accatctggg aaggcgacag ggcctttccg gcctacaaga
    30541 acagcgtaaa agacggtatt tacgagtaca acgatttcat tggcatgagt cttcccctga
    30601 actccggtgc ctcagccatg gtcctgtgcg cataccagga ccccgggcat atacgcgaga
    30661 tccttggatc catgaagctg gagaaaagaa cttcgaaaac aatgaccgat cttgaccagc
    30721 tgatggacga atacaggatc atacgcgaaa ggggttacgc acttgaggat gaaaccttca
    30781 gcctgggcat cctgtctatt gccgttcctg tttttgacaa ggatgggagg gtatggtcat
    30841 gccttgccct tgccgcacca aaggcccatg ccaccgatgg gaatgttgat gaatggatac
    30901 aagccttgaa ggaaggggct gaagagctgt cgtacaatct gcagttcaga cactagtaaa
    30961 gggaggcggt tcactgagag gagactattg ctgaatggga ggacaaacca gaaaaaaagg
    31021 agagatagcc atgaaaagaa atattgcttt tgcagttctt gccgtcttcg tccttggatg
    31081 cttcatagta accggggcgg ctaccccaag tctcgccaaa gacaagttga ccctcaaggt
    31141 cgcaggcatt tccgccccgg aataccgtgg aaccaaatca ctatacacta ttaaggatat
    31201 tgtcgaaaag gggaccgagg ggaggatcat tcttgatatc ttccctgcta accagctggg
    31261 agattacaca caggtctttg aggagatccg gcgtgggacc attgagatgg ggctcatatt
    31321 cttgccgagc caattcgacg tcatgcttga agtaggttcc cttcctttcc tggccgagac
    31381 gggagagggg atgcaggaac agctatcccc cggctcctat gtctactcga tcattgagga
    31441 ttctctcgac aaacttgggg tcaggcttct gaggatctac ggtgatggtt ttgtgggtgt
    31501 agggcttgcc aggaaaccaa aagatccggt taaagcaggc acgccaaagg atgccatgat
    31561 cagggtcgct ccgcttgcgg tctacaagga gacagcggag gatctcggat tcaggaccgc
    31621 aagcatcccc tatgccgaca cctacagtgc aatccagaca ggggtctgcg acggttggat
    31681 aggaggatcg tcacagataa actatctcag cttccgtgac gttataaagt attacattcc
    31741 ctacaactgc ctcttcgacc agacaggcta cataatcggg aaggatattt ggaacaagat
    31801 ctctcccgag gaccagaagg tcatccttga cgccgttaat gaacaggccg acaagagctt
    31861 tgccgacagt aacgctgagg acgtcgagta ccaggagaaa ctcgctgaag ccggtgtgga
    31921 gataatcccc atctccggaa gtgaaatggc cgaacttgcg gaatacattc gcaataccac
    31981 ctggcccaag ctggagagca cctacggcaa ggaaaccctg gacaagatca aggaatcgct
    32041 tcaatagacc atccctttcg ccgggcaggt tcacaacctg cccggcgttt ctctggaggg
    32101 gtcaaatgtg aatctcattg gatcgatcaa aaggtcgctt ttctggaagt cgatcttggg
    32161 tctgcaacgt attctcttgg tactaaccag ccttttcgtt gtgctgataa tgtgtgccga
    32221 ggtggtcctg agatatgtgt tcaaatcaga tcttttcggg atcgaggaga tagtggtaat
    32281 agcagctttc tggctctatt tcatgggcag ttcctacggg gtcttcgaga agagccatgt
    32341 caaggccgat atcatccccc agatgctgag acccagggca agagctttgc tcagcgtact
    32401 ggttaatttc accgtgtcgg ccctctgcat tctgttcaca gtatgggctg tagggatggt
    32461 ccggtacagc attacctgga tgccaagaac aaccgggttg aggataccca tctacatttc
    32521 ccaatcttca gttttgttgg ggtatgccct tatgtccatg tattcgatcg tctacttcat
    32581 tgaagatctc tttgtattcg tttccatggg ccgggagagg gaggcctgat atgagatctg
    32641 tcatctgttc ccgttccgcc aagcacgatc gtgacaatga tcctgcagac cgcgttagga
    32701 caggaaatag gagggaatgg gcatggcgat aaccgttgcc cttttgctga ttattattac
    32761 ccttcttctc gggctgcccg ttcccttctg cttcatgctc tccaccacct tcatggtggt
    32821 aacccacggt tacgacccat ctttcctctt gccatacggg ttcagccaga tgagctccat
    32881 cgtccttctg gctatcccgt tgttcataat ggtgggcgga atgatggaca gggggggaat
    32941 cgggacttcg ctggtgaatt ttgtggaaat ttttgtggga cgcatcaagg gcggtctggg
    33001 ggttgttgct gtcgtttcct gtgccgtgtt cggttcgatc tccggaagct gtgcggcgac
    33061 cctttcctgc attgggtcga tcatgttccc gaggctcagg gaaaagggct accccatggg
    33121 gcacagtgca gcccttgtct catgcgctgc cccgctgggg cttttaattc cacccagctc
    33181 ccagatgatc ctctacgcct gggtcggaca gcagtcagtg ctagcctgtt tcctcgccac
    33241 ggtggggccc ggtattgttc tgatgatcct gttgagcctt ctgaatctga tcatgcttcg
    33301 gaaagacccg aacatagagg tcgttcctgt catgtccagg ggagagaagc tttccatgtt
    33361 cagagatcgg ggcaaaagag ccgcgcccgc cctcttcatg ccctttattg tgctcggtgg
    33421 tatatacggc gggataatga ccccgacaga agcggcagcc atggcagtcc tctactcgat
    33481 acctgttgga ttctgggttt acagaggtct taaccgcggc aacttcatcg aggtcatcat
    33541 tgagacggcc actacgacgg gggtcgtcat gctgatgatg tactgcataa tgatgttgag
    33601 caggatctac gttcaggaga gactccccga tatgatcatc aataccctca cagccgtctc
    33661 tacgaacagg tacgtcattc tgttcatgat caacatcttt atgataatca tcggtatgat
    33721 catggacgat gtgagtgcca tgctactttg cacccctatt ctgcttccgg tcgtcaccgc
    33781 actgggagtt cacccgatcc atttcgcggc tatcatcggt accaatctgg ggatgggtaa
    33841 tgtaacccct cccaccgctc cgacactgta ttttggtggc cgtatggcga aaaccccggt
    33901 ctcgcagatg atgaaacctg ccctggtatt tatcaccttt gcgtggctgc cgacactgct
    33961 cgctgtaacc tacttgcccg aactcgctct ctggcttccg cgactggttc tagggggccg
    34021 tatataggag gttaaactat ggggaaaatg gattatcttc aagaaaaacc gatcgctgtc
    34081 ctcggaggag gcgctactgc ccgtggccat gcggcatgcg cggcccttgc cgggcgtgag
    34141 gtcaggcttt atgagctccc ggattttttc gagggactgg ggtgcataaa ggaaaaccgt
    34201 gagatccggc tcagcgggat ccaggaaagc ctgtacgggt tcaaacggga aggcctggca
    34261 aagatcgacg ttgtgacatc cgatatggaa gaggctgtaa aaggggcagg gataattgtc
    34321 gtcagtttcc cggcagtcgg ctacaaggcc ttcctggaaa aactgatccc ccgcctggag
    34381 gacggaatgg tggtacactt caccaccgcg aatttcgggt cgcttataat gcgaaaaatg
    34441 atgagggagt ccgggtgcag taaaaggatc gtaatcggcg agtggagcag ccagccctac
    34501 gggataagga tcaagagcgc cgggggacag cagatgcctg aagtgagcgt aaactactgg
    34561 gccatttccc tgagaggttc tgcccttccc atgatagatc aggagaagtt cttcgagtca
    34621 aagcaatatc ttccttctct tgattcggtt gttcatcccg tcaggggtga caccgtcgcg
    34681 gacataggct tcagcaacgt gaacccgatc ctccactgcc cagggactat cctcggcgtc
    34741 gggaccatgg agaactgggg tgttatttac ggaggggaca agcacgattt ttccatatac
    34801 tctcatgcat actgcccctc tatctcaaag gtgcagctgg ccctgtacaa ggaggagtgc
    34861 gccatcgcgg aagcaatggg agtgggtatc cagcaattct cagaagagtc ctttttctca
    34921 aggtccaaca ttctcggttc ggaacacatg gggggaaaat tcaaggtccc cttcgatgaa
    34981 cagtacaaac tggctctggg aaccggccct ttttccattt acaatcgcta catcactgag
    35041 gacattcctg ttggctgtca catattccgg gagttgggga aaaagttcgg agtgaaggtc
    35101 ccggtcatag aatcgatgat aacccttgcg tcggtaatga ccggagtcga ttactggtcg
    35161 gaaggggtta ccctcaatga cctgggaata gagcatatgg acagggaggc acttaacgcc
    35221 taccttcgtg aaggaacata tctttaactt cgaggggctt cttcgaagat gaaggagccc
    35281 ctcggaattt tccgtggtct gatctttcaa gttaggagag taagaacgag atggaatggc
    35341 aaggactgtt caatggatct gctacgaacc ggcttgtgga ttccgcgatc gcttcgatca
    35401 tgaagatcgc ctcggaagag aacgccatgt ccctggctgc cggcgaacct ttcggggctc
    35461 tctaccccgc ggaggaattg agagactgtt taatgatatc tttttcaaag gagctgcctg
    35521 tctggggata cagcaactca cggacaggga tcgccccctt gcgccagtgg atcgcagact
    35581 ggatggccag ggacggtctt cttccacaat gggcaaatag cgacagtctt ttcattacca
    35641 acgggtcgca ggaagggttg tacctgttat gcgaatgtct cctggagccg ggggataccg
    35701 tgatagtaga atcgccatcc tatccggaag cgtttgggat tttcaggaaa ttcggggcaa
    35761 aatgcctgag cgtacccctt tcagaggaag gtcccgatat aggggttctg gaagggattt
    35821 taaaggagaa ccgggtcaag ttcttttaca ccatccctac ctaccagaat ccaactggtt
    35881 tttcgacccc tgacagcaag aaacagagcg tactggacct tgctctcagg cacgggttcg
    35941 tgataattga ggacgaccct taccgcaacc tttccttcga aggtcgaccc gaagggacct
    36001 atatcagggc cgcaggagat aaaaagaacg tagcctatct cggtagcttt tccaagatcg
    36061 ttgcacccgg ccttcgatgt gggtgggtgg tcgctcccca accggtaata gagaaaatgc
    36121 tcaagatcag gatcatggga acgctctgtc tcccggaaat tttgcattat ggcctctggg
    36181 agttcgtaaa tagcatcgat ctgacagaat acctcgaagg tctttcaagg acctatcggc
    36241 aacaccggga tgccctggtc tcctccctga tgagacatgc cgggccccat ggacttgagt
    36301 tggccgtacc gaagggagga ttcttcctct ggggtagggt gccctggatc gaagacatga
    36361 agagtttcgc gctgtatgct atcagagaag agaagatagc ggtgatccct ggaaaggagt
    36421 ttttcctggg aggacatgtt gaggaggata ctgtcaggct ctctttctcg aaggtcaccc
    36481 cttccatggc tgaagagtgc gctcgtcgcc tcggcacggc tttgaagaga ttccgggatc
    36541 tttgatactt gcctgaatgt cctttcccga acgacaggag gatgctgtat gggtgaccat
    36601 tcaaaaagag agttgtatgg cctgctttta aacctggtcg ggattccgag cgtctctccc
    36661 tccgcagaga gcgagaacag gatcgcccgt ttcatccacg acagttttca ggatctgagc
    36721 tattttcagg agaaccgggg cgatctacgt cttctgccgc tggagggtga cccccttgga
    36781 agacacctgg tcttcgccat tgtaagggcc cggaagagaa cggccgatac ggtgatcctt
    36841 atgggacaca tggacgtggt gggaaccgag gcgtgcggct ttctggcgcc cttggccttc
    36901 tcccccgagg agtacacaga acggatagcc tcggtggcga tcccccggga tgcccgcaag
    36961 gacctcgagt ccggcgaatg gctcttcggg aggggggtgg ctgacatgaa gagcggtgtc
    37021 gccgccggga tggacctgct catggaagca gccgagaacc ccggtaaata tgacgccaac
    37081 gtggctgttc tcttcgttcc cgacgaggag aacaactctg gcggtatgct ttcgggttca
    37141 tcctgcctgg cccgtttcca gaaagaggag agtctccggt acctggcctg catcgacctg
    37201 gagcccacct tcgccaccgg ggacgatgcc aggccgacca tatatctggg gagcataggc
    37261 aagataaacc cctttttctt ctgcactggc aaggaaaccc acgtggggga gtattacgag
    37321 gggttctgcg ccgcgccggt catttcgagg atcaacctga tgctcgacgg gaaccccgag
    37381 tacgcagact ccctcttcgg gaagtcatac ccacccttcg gctgcatgag gcagatggac
    37441 ctccggaggg agtactcggc gactatcatg accaaagctt tcgccttcta cagctacctg
    37501 acgtcaacca ggcttccggg tcagatcctg tcggaaatga gggggatcgc catggaggcc
    37561 cttgaggcct cgatcgaaaa gtaccgggag aacaccagtt cctttgcggc gatgggtggc
    37621 accacctcga aaccccgcga ctgggagccg acggtggtct ccttcgacga gctttcggac
    37681 atggcggaga aggtcctggg cgacgggtat gctcgcttca tggatgagac ggtcggttcc
    37741 gcccctgagg gttccgacga gagggtccgt gccgcactcc ttgtcgagtc catggtggaa
    37801 ctctgcggga taccgggacc gatggcggtc ataggttttc tccctccatg gtacccccac
    37861 aggtccaacc tgggagagac ccggggcgag aaggtggttg ccgatgccgc ttccgcgaca
    37921 gccggggaga tggagaggag gttcggcctc ccccttgata tcaggccttt ctttgaggga
    37981 gtctcggacc tcagctactg cggcttccag ggtgacagca gtgagatgga ggtcttcgcc
    38041 aggaacatgc cagggtgggg tagaccttac aggatccctg tcgaagcact ggctgaactg
    38101 gacattccga tactgaacct gggacctgtg ggcaaggacg cacacaagaa caccgaacgc
    38161 attcaccttc catttatgtt ggaggttttc ccgaatatcc tgcgtttcgt ggtggaaagg
    38221 atcatcgaag ggtaccgctc ctgatcgtca ttgccggata aggaatgcgg gggtcccctg
    38281 aaggggaccc ccgtcctgtg ttacaatacc tgttcccctc aagagggtga gatccagaaa
    38341 aggaaagggt gatgcaaacc atgacccttt ggctgaaaag gatacttgga cgtgtcccgg
    38401 aactccgtcc gtctcccggg gtcctgaaag agtgggggat gatccacctc ggctgcttgc
    38461 ttgttgcggc ggggatcacc tggttcctag tgccgaacaa tattgccgcc ggaggcgtca
    38521 gcggccttgc catggtcctt aaccgcttcc tccccggggt cccggtgggg gtactcatga
    38581 tggggatgaa cgtggtcctc ttcgtcatag ggttcatatt catcggattt ggattcgggg
    38641 cgaaaacgat ctattcgagc cttttccttt caggggctgt ctggttgctg gagaggttct
    38701 acccggtgtc tggaccactg acggaggaca tcatggtcca gcttgtcttc ggaaccatcc
    38761 tgggggccac gggaatggcc attgtcttca accagaacgc ctcgacgggg ggcacggaca
    38821 tcaccgccaa gatcctaaac aagtacctga acatcgatat cggcaagggt gttctcttgt
    38881 gcgacttctt catagtgctg gctgccggtt gggtcttcgg gatccggaca ggtatgtatg
    38941 cgatgctggg ggtcattctc aacggattcc tggtggacag cgtgatcgta ggactcagga
    39001 tcaacaagta cgtggtggtg ataagccgtc agagcgaggc gataaggcgg ttcatagtcg
    39061 aggagctcga gagcggtgcc accatctacg aggggcgggg aggatggtca ggggaaccga
    39121 aggaggtcat taccaccata gtgaacagaa gaaagttcat caggctccgc aatttcatca
    39181 aaaccgtcga tcccagggcc ttcatcagcg tcaacaacat ccacgaggtt ctgggtgaag
    39241 gttttgaaag gctggcctga agggtccatc gataacaata ttcaggttac aatctccgaa
    39301 ggggggtgat gcggctggaa gaccgtcccg taagacttgt tctctacaat acccgatacg
    39361 gcactggaac ggggtgggcc taccatgccc ctttcccctt tgccggaacg ttgaagacca
    39421 ccagggagcg ctcctcctcg atcgcaaggt tcattgcctc cctcgagcct gacatcgtcg
    39481 gactggttga gaccgatgga ggatccttca ggcacggagg aagaagtcag ccggagtacc
    39541 tggcggagac cctgggacac agctgggttt tctcatccaa gtactgcatt cccaggatct
    39601 ccggatgcgt ccccgttttc cgccaccagg gaaatgcggt ggtgacgtcc cttcctatcc
    39661 gggagacctc ggtccgcaga ctgaggaggg gcatcaagaa cgcagtgata gagaccggtt
    39721 tcgacgactt cgacctgaag ctggtccacc tgtcgctggg ttccggcgct aggcggcaac
    39781 agatcctcga gctcgcggca atggccaggg agcgtaaaag acctcttctg atagcggggg
    39841 atttcaacgc cttcggaggc ggtcccgaga tcgagcccct tatcgaagct ggtcttatga
    39901 atgccgatac ccttggcata cggacctatc ccagcggcag gcccaggctg ggcctggatc
    39961 tggtactgca ttcgccggag atagatgtca ggaggatcga ggtgccggat gtgcgtctct
    40021 cggaccacct tccggtggtc tgtgatttcg ccctgtccct gtagtgcttt ccggaggtga
    40081 ccggagatgg acctgagcct gcttctgata ctgatccacg acctctcatc ggtatccata
    40141 agactggcga ccattgcgct gataccacgg tggcactcgc cctcggtggc catggcgtgg
    40201 ctgct
//
LOCUS       DAOTIQ010000008        38888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1114_length_38888_cov_7.452450, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000008 DAOTIQ010000000
VERSION     DAOTIQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 38888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38888
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_38888_cov_7.452450"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            654..750
                     /locus_tag="P5541_02240"
     tRNA            654..750
                     /locus_tag="P5541_02240"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Sec,seq:tca)
     gene            798..1619
                     /locus_tag="P5541_02245"
     CDS             798..1619
                     /locus_tag="P5541_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HRV70824.1"
                     /translation="MQKKRFSLKGYSYPLTPGGKSSVVGDFPWHYGTEYLNILFRTDP
                     DVIASYLPHPLKPGPEPDLAYVAFSRWWSVWEDQRDMACINPERTQYMEAAIWTGCSF
                     EETVGQICLHIWVDNDFSMARGWFMGFPKKLGEVYMTDYHPLNPAMPKMGIGARLKGI
                     ASAHGERLFEGSMDITGKCSPGELPRPMGLPLLHVRHFPSIVKDAPPSVLELVSLGAE
                     DYRYGENVWKGKGSLKFYPSEIEEHFPLAPREVLGAWHYTSGYTFPGGEVLFSWL"
     gene            1742..2824
                     /locus_tag="P5541_02250"
     CDS             1742..2824
                     /locus_tag="P5541_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV70825.1"
                     /translation="MRKILLAIVCISLVMGASASFAMEPVAAKDLKAGFVYVGPVGDG
                     GWTYMHDIGRLEMEKAYPGMESFFVESVPEGPDSVRVMETFIRNGANLVFATSFGYMD
                     FVQDVAAKNPDVVFMHCSGYKTAPNVGNYFGRMYQARYLSGLVAGKMSKSNVIGFVAA
                     NPIPEVIRGINAFTLGARKTNPDVKVKVVWLFSWFDPAKEKEATLALIDAGADVIAMH
                     ADSGAAPQTAEEAGVYVVGYNNDMKSYAPTKHLTAPIWNWGMVYKKIAGEVIDGTWES
                     HDIWWGLKEGLVGLAPLSPEIPDDIVKLVEAEKAKMVEGKWDVFSGPVKNQKGEEMVK
                     KGETMSDGDMLGMNWFVLGVEGDIPQ"
     gene            2959..4515
                     /locus_tag="P5541_02255"
     CDS             2959..4515
                     /locus_tag="P5541_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70826.1"
                     /translation="MRDIVKSFVGVKANDGIDLDLQRGEVHTLLGENGAGKSTLMNIL
                     CGLYTPEKGRILIRGEEMTFRSPRDAIDAGIGMVHQHFMLVPAQSVWENVALGLRDLP
                     FVPDKNEVIGRIESIREKYGIHVDPLAPVWQLSIGEQQRVALIKMLYRDAEILILDEP
                     TAVLTPQESRALFSTVRQMVSEGHGIIFISHKLDEVLEVSDKITILRKGKKVATVPAS
                     RASKESLAEMMVGRKVVFQIGKTLQNPGRVVLETRDLFVRGDRGRMKVSGFSINVRKR
                     EILGLAGISGNGQEELCEALAGMRKTEGGGILVDGTSMEERETRDFQRRGIGYIPADR
                     VGTGLVTGMNLRENIALRKYWRKEFTRFRFFVHWENIAGRTGQLVERFGVVNPGLGYP
                     ARMLSGGNLQKLMLARELSDDPKVVIAVQPTWGLDVSATQFVRETLLEQRDRGAAILL
                     VSDDLDEILSMSDRIAVIHDGKLMGLVEHPDTVSEEQMGLMMAGSPLEEFEVPPDSER
                     ESTGKAVTLQ"
     gene            4515..5588
                     /locus_tag="P5541_02260"
     CDS             4515..5588
                     /locus_tag="P5541_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70827.1"
                     /translation="MRYRIVVRRRYYTSLAFETLLPVLSVMVALFLAGLALAFFGVSP
                     VRAYIAMFRGALGSWYGIGEVVVKSIPLVIAGVAVALAFRMKIWNIGAEGQIYAGALA
                     ATAAVRYLQTDSRFAMLAVMLMAAFLAGGCWGGVAGYLKARWNVNEIITTLMLNYVAI
                     HLVEYYIYGPWRDPSSLGFPMTAPFPASARLPLFFDSRVHLGIFAAIILVLVYRLVIR
                     WTRWGFEIRAIGDNPKAASYAGILSSRNIILLLFVSGAVAGMAGMIEISGLQGRLQHG
                     FSPGYGYTAIIVAWLARLNPWGIMIVSFFMGVLLVGGDTLQVVMRLPVASVQVIQGCI
                     LFAVLASEFARNYKIQVTRKERE"
     gene            5590..6519
                     /locus_tag="P5541_02265"
     CDS             5590..6519
                     /locus_tag="P5541_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70828.1"
                     /translation="MDILIPILAAAVRSGTPILYATLGEILTERSGVLNLGVEGLMLI
                     GAFSGFMAAHASGNPWAGVAVAFLAGACVSLVHAFLCVTVGANQVVSGLAVTMFGTGA
                     SSLLGRKMIGETIAGFGKLQIPFLSGIPVVGPVLFNHDPLVYVSYGLVIFLVWYIFHT
                     RPGLNLRAVGESPKVVDAMGLSAARLRYFYTVLGGGLAAVGGAYMSLAYTKMWGEGMT
                     AGRGWIAVALVIFAIWHPARAVLGAYLFGGVGAMQLRIQAAGTTFPAPLLMMLPYLLT
                     IAVLLLISIRKGKGILLGAPAALGVPYSREERG"
     gene            complement(6548..7051)
                     /gene="gpt"
                     /locus_tag="P5541_02270"
     CDS             complement(6548..7051)
                     /gene="gpt"
                     /locus_tag="P5541_02270"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRV70829.1"
                     /translation="MSGTDQKYRKTYPVSWKQLHNDCRALAWRLLEIRKDWDKIIAVA
                     RGGLVPAAIIARELDIHNIDTICVSSYTVRSQGESKVIKKTFGESEKCLIIDDLVDTG
                     KTARIVRNMLPMSHFATVYAKPDGRPLTDTFITEVSQETWILFPWDSEPQYQYIEPIA
                     DISRRGA"
     gene            complement(7074..8492)
                     /gene="cysS"
                     /locus_tag="P5541_02275"
     CDS             complement(7074..8492)
                     /gene="cysS"
                     /locus_tag="P5541_02275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRV70830.1"
                     /translation="MTLRLFNDLTRKKEIFEPLESGKVRFYSCGPTVYDYFHIGNARP
                     FIVFDSLRRYLESCGYEVLYAQNFTDIDDKMIARAAQLEMTVPELASRFIAAYYEDAD
                     ALGIKRPTFSPMATEFIPEIIALVEKLVEKGNAYVVDGDVYFEVQTFPEYGKLARQSL
                     DDLLSGARVDVDPRKRHPLDFALWKAQKKGEPSWTSPWGEGRPGWHIECSAMSMKLLG
                     ETIDIHAGGSDLVFPHHENEIAQAEAASDKPFVRYWIHNGYILLDQEKMSKSLGNFLT
                     AREARERFSPDAIRLFMLGAHYRSPISFSDESLKQAESGISRIKNSVTDLLSAPVTDK
                     ATSNINGENSLVESVSSFNSSLDDDFNTAGAIGTLFEVIRTINLAVAHGQAVSPVFFN
                     NAISFLEHCDKVLGIIGLEYLKKGSPEPGDPEIDEMVAAREIARKNRDFAAADKIRQE
                     LSARGIAIEDTPQGARWKRITK"
     gene            8666..10141
                     /locus_tag="P5541_02280"
     CDS             8666..10141
                     /locus_tag="P5541_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRV70831.1"
                     /translation="MAAVDLSRKISELPLRPGVYTFLDEKSEVIYIGKAKSLRKRVQS
                     YFRHSGFASPRLRKLVESVSDVSVIRTESEAEALIVEARLIKQYQPFFNVDLKMNERY
                     PYVKVTSEPYPRIVITRTRDKDGSVYIGPFVSVRELRQMFRMIERYFPLRNCKSDSSR
                     GSWRDRPCVRYELGKCPAPCVGTGNPSEYRERVADVVLLLKGQSAELVGRLRDRMDKA
                     SENLDFETAARLRDTIRSLWKMSRQHVSLALSEPAEREVWKGLLGLQKVLSLPSVPWR
                     IEGYDISHFAGGESYGVCVVFEQGTPNPDLYRKFRIKTVEGVDDFRAVAETVGRRFSR
                     LKSESGSLPQLVIIDGGEQQLKFVTERLKELGMEDIPCVALAKREELLFLPDRTVPLA
                     LPNGSMELTILQRVRNESHRFAITASRNAGRKRLSRSALEDIPGIGKIRAARLLSQVG
                     SMERIASLDPEDLSRVEGIGEKRARMILMHLKGVVETNDNG"
     gene            10128..11231
                     /gene="ribD"
                     /locus_tag="P5541_02285"
     CDS             10128..11231
                     /gene="ribD"
                     /locus_tag="P5541_02285"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRV70832.1"
                     /translation="MTMADRQIDEFYMRMALSLAIRGTGGTSPNPLVGSVIVDESGIC
                     GWGYHSAPGYPHAEAEALSMAGKKAAGATLYVNLEPCSHFGKTPPCAPRIIEAGIKRV
                     VAGIGDPDPRVSGRGFKMLSSAGIDVREGVLEKECRKINRGFLSRVERNRPWVTLKAA
                     VTLDGTMALGSGESKWITNEQSRARAHLLRGCNDAVLVGRGTVECDDPLLTVRKVSGR
                     DPVAVVLDPGLSMSSGSNLAREGTIVFGLAGAGLGKRRSLERQGCRVFLVESDEKNRI
                     PIIPMLEVLGSKGINNLLVEGGPLVAGSFLEKRCFDSISFFVSPRIAGEGRKIGEGFI
                     ISGFDVSAEVSITSAVDVEGDLWIEGEMRCSPD"
     gene            11216..11869
                     /locus_tag="P5541_02290"
     CDS             11216..11869
                     /locus_tag="P5541_02290"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRV70833.1"
                     /translation="MFTGLIECTGVIASSRQRGDVTEIDIRDVPFATSLEKGDSVAVS
                     GACLTVTRISGTGFSVEMMPETRVNTIPGLTSQGYRVNLERSLSSSGRFEGHIVTGHV
                     DTVARVSGITELGRTRILSIDAGKKYMKYVPRKGSIAIQGVSLTVIDSSDGIIRTGLI
                     PETLSRTTLGDLEPGSAVNLEFDILCKYIEGLLSGNGTEPEKGSRINLDYLGEIGWA"
     gene            11892..13136
                     /locus_tag="P5541_02295"
     CDS             11892..13136
                     /locus_tag="P5541_02295"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRV70834.1"
                     /translation="MEGNTGEKFFCTAEEAIEAIGRGDMVIVVDDESRENEGDLVMAA
                     EMVNRESINFMASMARGLICVPVDAEVAGRLGLEPMVQDNTDRHSTAFTVSVDAREGT
                     TTGISAAERAITARLIADPAATADKFRRPGHMFPLIARNGGVLKRAGHTEAAVDLARM
                     AGKTPAGVICEIMNEDGSMARLPDLVDYASKHNLMIISIEDLIRYRNRKDRLVEKVAV
                     VHLPTEYGDFMAHAYRFTLDEQKDRVHLALVKGNLARDEATLVRVHSECLTGDALGSL
                     RCDCGPQLHSALRMIEEEGSGVLLYMRQEGRGIGLLEKMHAYELQEKGLDTVEANEAL
                     GFKPDLRDYGIGAQILADLGLSRIRLITNNPRKIIGLEGYGLEVSERVPVVISPNCYN
                     EKYLQTKENKMGHMLHLRDIIG"
     gene            13169..13663
                     /gene="ribE"
                     /locus_tag="P5541_02300"
     CDS             13169..13663
                     /gene="ribE"
                     /locus_tag="P5541_02300"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRV70835.1"
                     /translation="MKIIQGKLVGKGQRFIIAVSRFNELISSRLLEGAKDFLLRHDVS
                     PGDIDVVWVPGSWELPLVAREFALTGKYDAVIALGAIIRGDTPHFDYVSAEVSKGLAM
                     VGMEHRVPVSFGVLTCDNLEQALLRAGSKSGNKGSDAAAAAVEMADLMKQIRSVKYGE
                     EKDA"
     gene            13656..14942
                     /gene="serS"
                     /locus_tag="P5541_02305"
     CDS             13656..14942
                     /gene="serS"
                     /locus_tag="P5541_02305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRV70836.1"
                     /translation="MLDIKLFRENPEDIKSLLSSRNGKFPVDEIVSLDSERREILTKT
                     DDLKAERNRGSREVAEIKRSGGDAASIMEKMKSLGDRIRENDAKILELDSRIQSMLLE
                     IPNLPHSSVPAGEDESANEEIHSFGEIPVFEFDPRPHWEIGEELGIMDFERGVRLAES
                     RFTVLKGLGARLERALINFMLDLHTNDHGFIEIEPPFLVNSDTMRGTGQLPKFAEDLY
                     SCSCDDLWLIPTAEVPLTNLHRDEIIPEDELPVYYTAYTPCFRREAGSHGRDVRGMLR
                     QHQFDKVELVKLCTPENSFDELELLLRAAEKVLEKLEIPYRTVVLSSGDMGFSASKTY
                     DIEVWLPSQNKYREISSCSNCTDFQARRMNTRYRSRNGGKVQFVHTLNGSGIAVGRCL
                     IAILENFQQKDGSVLIPEALVPYMGGVRSLENPREQ"
     gene            15015..16751
                     /locus_tag="P5541_02310"
     CDS             15015..16751
                     /locus_tag="P5541_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRV70837.1"
                     /translation="MPVDVREIQATLFLALSLFVFFFLLQRISKRSLERDQYQFTKDV
                     GLLAAWAICGIWASDPSVRLVVALGLVSAVMGVGQRVAPRVPWAAGFFAVGAVLASAG
                     MRISFIGIPGGDYLFLNDYSSLLVTTIWVGMFPLLLQEMDQIPGLAGFLSAITWVIML
                     IVTSLSRQHISGAFYMTLMGVVILAVFWSRHGHSYRRLGEPLAALWGTLIAGTSIIGV
                     SKGVTFSTLMLIPLGLFAIPIAEASFNIVSRAFSSRPQGTLFLYRRLVNRGLDHTPAV
                     RMVGVLCAVLGAVVASVQLRDEKTALAAFAASATVMAGSLLVMYGGSRGRNVPVARTG
                     IWGVPVDSISVDYALGKVRSWVNLDHGGRYIVTLDALSALRAREDKKFRDLVRGADLV
                     LPDGKGLIAALSFLGTPVAQRIPGVEFVEKMCRLASTEGWPVFFLGGAEGVAAKAALI
                     LERKYPGLVVGGISHGYVESGDMPSVVDNIKSSGSRFIFVGMGVPCQEKWMSEYGKTI
                     DRSVSIGVGGSFDVISGNLKRAPSAVQNIGFEWLYRVIQEPWRWKRVKRLPEFVIKVF
                     LTRIGFSSLKGD"
     gene            16762..17223
                     /locus_tag="P5541_02315"
     CDS             16762..17223
                     /locus_tag="P5541_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV70838.1"
                     /translation="MNAEHLMHRDLSAVSEDDLILDAVHLLYSQRLSGVPVIRDDWVL
                     TGYLSEKDILKGATPTYLEILAQSSFLDDSEGDLIQRLRQIGDLPVRDLMNRDPVFVR
                     PSESLMTVVDLMLRKGLKRLPVVDGRKLVGVIDRGAFCEFMMEGQNFNDSR"
     gene            17210..17584
                     /locus_tag="P5541_02320"
     CDS             17210..17584
                     /locus_tag="P5541_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70839.1"
                     /translation="MIQDNLEAKNPDESGACLDEFRRELEVRLEIEKTKMKKEQQEAL
                     ARIEQISLNSIKQAEAEWENYETLLESARSELYRRSRDEMSRLFSDSCSLSGEEDFVT
                     RVIAKILPFDDPEKNNNGGSGN"
     gene            17581..19488
                     /locus_tag="P5541_02325"
     CDS             17581..19488
                     /locus_tag="P5541_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRV70840.1"
                     /translation="MIRDMVSMTLRGLLADRSMVIETLHDEGVLHFQEETLEVETGES
                     AAGLKILRAKALGLLEALEWREWADITSLNLQEKRKALSSPDSTIIDGIGESLDSFSS
                     RLNELKTVRSELEGRMNSIRGTLKTLEHFRSFIKDNIEKGNSISIWWTQRGNVAEMVS
                     MVQKRIHNRDPLGFSDSVTYHTAVAAGGEAVAAIGVHPDNIEDVRGVMTENDCLAWQF
                     PSEFEGFRYDEAIRQMEIVQKDIPRRIQETEKNLQNTKNTWGPRIGALYVILDEKVQE
                     IFVEHGSETSGEIFKLEGWVPLDEKDRLTTSLREKFDGRILLSWKEPSPGEWANVPTS
                     LKNPRWFKPFEIFLALMPVIGYRGLDPTIVIGVFFPFFSGCMIGDAGYGAVILFLAAW
                     LRRMKTRPVAVDIGSILLFVGSWSIAWGIAYGEFFGDLGHRLFHMEPLWLERSQVVIP
                     VMVFTVSLGFAHVLLGFVLGVVQGIKTGQKHLWVERLGNILILAALLSAMIVVKGWLP
                     GKMFSLTVTFLLLGLALLLAGGGMGGLVESLGSIGNILSYVRIAAIGLSSAILAMVAT
                     SFVDSMGVSFVGVFLALTIHLLNFVLAVAGSGLHSARLQYVEFLGKFYTGGGTRYKPF
                     SRRKPELWKKR"
     gene            19473..19691
                     /locus_tag="P5541_02330"
     CDS             19473..19691
                     /locus_tag="P5541_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRV70841.1"
                     /translation="MEKALIAFAAALAVGIPAIATAYAQARIGSVGAGTIAEKPETGG
                     IIIILEAIPETMVILGFVVAVMLILQFA"
     gene            19812..20375
                     /locus_tag="P5541_02335"
     CDS             19812..20375
                     /locus_tag="P5541_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRV70842.1"
                     /translation="MRLESGRFISERIFLVRRETEKRVHHIRERQRKQYETTLEGEKL
                     RVLLDVRMSALQEIADLFEAISLKIEKELLLLRENHSGYPKILENLFYEALERLGESA
                     VVIVAPGDRALIPQDPLVSSVEEQESLLDCGGCVVVDAQTRTIVIDNSVRTRWNSLKR
                     EFMREFSGKYADVLQGFDRFSRKLRIS"
     gene            20326..21378
                     /locus_tag="P5541_02340"
     CDS             20326..21378
                     /locus_tag="P5541_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRV70843.1"
                     /translation="MFFRDSTDFPENYGYLNARLRAHFSDFLGEDDYRKLAGKSLQGI
                     ELFLLETRYGKSFRKGLVEQPLSSHGRIERALADTSAEVMNHTRNMAMGEPEDLMRVL
                     LARADLHNCRLLLRFFFTGAISGGAPLWHGYGTLPVSFFERLWKSRSITHVIDKCLAF
                     PHPMSRSLGKACSELQKGSPLPVSERVLLVEIVDFFRQIIEKHRTKNGDVVREFLGRT
                     IDIWNINIWSRAISGTMKIEKAKGLYISGGRFLAADKLENSSGPEELFRSTPWESMLS
                     GSRGRSVGFVVSRATRIFWNWQMSLRRVDPLGIEVAVAFIACQLVEWHNLNALVVGID
                     MGLSVESIMDKMILQG"
     gene            21388..21663
                     /locus_tag="P5541_02345"
     CDS             21388..21663
                     /locus_tag="P5541_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRV70844.1"
                     /translation="MKESEGRGYVLGRQLFIELWSLLGMESMVCETVSEFPACFERLQ
                     SESIAFVLVESDWFENIPEIYKRKIRTGESPVWVEMPSLKSSVKSWE"
     gene            21668..23440
                     /locus_tag="P5541_02350"
     CDS             21668..23440
                     /locus_tag="P5541_02350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRV70845.1"
                     /translation="MSTLGSIKSLAGTVIRASLSSPVTNFEVARVGKERLLGEVIRID
                     GNEADIQVYEEIGGVHVGEPVEFTGELLGIDLGPGLLGSVLDGIGRPLEFSEEGGAPP
                     VFLKRGYYVNSLDQCKKWEFNPAVAEGDTVSPGDIIGHVEEGKGFLHAVMVPPFTRPS
                     RVGMVLPPGSLVTVRDSVCELENGETIGLSQRWHVRRPRPVLEKLPFTEPLVTGQRVL
                     DTLFPVALGGAAVVPGGFGTGKTVTQQSLAKWCNARIVIYIGCGERGNEMTEVMEEFP
                     QITDPHSGRPLMERMVLVANTSNMPVAAREASIYLGMTIAEYYRDMGLSVALMADSTS
                     RWAEALREISGRLGEMPGEDGYPAYLGSRLAQYYERAGRASLLGTPGREGSITIFNAV
                     SPGGGDFSEPVTQSSLRLSGAFWALDKRLAEARHFPSINWNRSYSLYESGLRTVFEKR
                     LGKQWSVGASFLKALLDREAELLSLVQIVGRDALSEEDKWQIEMGEIVRVGFLQQNAF
                     DPLDAFCSTEKQMGFTKTLMDLNALVSERLGKGVFHYQIQELGLVRKVLSLRKVGEKE
                     FPAASSKWLDEVERSLEKVESIAG"
     gene            23437..24819
                     /locus_tag="P5541_02355"
     CDS             23437..24819
                     /locus_tag="P5541_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRV70846.1"
                     /translation="MRYYREGTRLVEGISGPLIFVGNARNAGLGEVVTIETTRGNRTG
                     QVLQVDGDVCAIQVLEGTLGLNPSDTTVWFERDVVKVRLSEALKGQILDGSGVPRSGV
                     PIPYFDKEEPITGSPLNPAMRSSPNAFIETGISSIDVLNTLVRGQKLPIFSGSGLPAN
                     EIAAQIVRQARVPGNDRSFLVVFAAIGITEREARFFRDSFEKTGALFNGVFLVNLASD
                     SAIQRLLTPRTALTIAEYFAFEKEYDVLVVMTDMLHYSEALREVSSAREEAPGRRGFP
                     GYLYSDLAGLYERAGCIKGKNGSITQIPIVTMPGDDMTHPVVDLSGYITEGQIVLDRG
                     LQARGVYPPVNVLSSLSRLMNKGIGEGKTLIGHRQVADQLFAAYARSRETARLRLIVG
                     DEGLTNTETRYLSFGNVFEKTFLDQGNQARKFGESLDSALKCLAELPDDELYRIPDDM
                     LEEVRGYRKE"
     gene            24816..25502
                     /locus_tag="P5541_02360"
     CDS             24816..25502
                     /locus_tag="P5541_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRV70847.1"
                     /translation="MSFETPVSRSELVELNRKIETMRFGCSLLERKKEALLRAIESDR
                     STFNLMRSEVEEMLSFVSYAYALVQIFEGESVMTLLSWGIEPREIRIVHHSLMGCRYC
                     EFFPRDDSKGTALAGLLMDPALASLHVDELLETLSKIEPVIWKYINLKAKLDALEMEF
                     EKTRRKVNNLEQEMIPAMESERSRIRLSLDERERQETFAVKKLLGRKRRSKVPGKEAP
                     VAKAVRRTKD"
     gene            25499..25978
                     /locus_tag="P5541_02365"
     CDS             25499..25978
                     /locus_tag="P5541_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRV70848.1"
                     /translation="MKILVLSVGKPKNPLFIKQVEDYRQRVSVRLPCKWDVVRESSGR
                     KPQNIILLEEGNALLKKVGERDYFVLLDESGRTMTSVRFSEWLFRRISETPGKTVMAV
                     GGAFGVSAEVRERANETMSLSPMTLTHEMSLLFLFEQIYRAVTIEKGGNYHHGNVVR"
     gene            26009..26317
                     /locus_tag="P5541_02370"
     CDS             26009..26317
                     /locus_tag="P5541_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRV70849.1"
                     /translation="MNMEEMLKQAQKLQSEIGKVQEELSREKVEGVSGGGMVKVTVTG
                     QGEILSINIEPEVIDPSEKEILEDLVLAAIHDGVRKSREMAQGRLGRFGAGLGIPGLG
                     "
     gene            26329..26958
                     /gene="recR"
                     /locus_tag="P5541_02375"
     CDS             26329..26958
                     /gene="recR"
                     /locus_tag="P5541_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRV70850.1"
                     /translation="MAVPGPVEKLMKLLAKFPGVGDKTARRFALFLLQQPRSYTDELV
                     SSITLMRDRTFSCSICGNLTTEDPCPICSDPFRDRKTLCVLENVEDMLSLEQAGIFSG
                     LYFILGGKVSPFDGEDIDQERFSHLSRYVEEGRFEEVIIATNPSIEGDLTFYAVLESL
                     GSASARITRLAFGLPLGGSIGYADRVTLHASIESRVVVSLSRDNEKGKE"
     gene            26963..28129
                     /locus_tag="P5541_02380"
     CDS             26963..28129
                     /locus_tag="P5541_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRV70851.1"
                     /translation="MAEGIVKLMLLLARIVCVLLGGVAGYQIGRFSLLQGWWPWTLFP
                     YILAPYFIGIFFFALLGYLVTPVFVRTLGFIGLSFEKNLESLSWNDISVALAGLIAGL
                     LVANLVALPFADLPGIGTYVAILLNVALGYLGVTIFLKRREEIHGMWSSIGGLKQKFK
                     IKGKKSRDGNGEQTHEYTIDSSEERSAIKVLDTSVIIDGRILEIVSTGFLEGPFILPR
                     FILNELQSIADSSDPTRRTKGRRGLDIMNEIQHLSDISVQIIETTLKDLDAGSVDDGL
                     VALGRELGAKVVTTDYNLNKLAQIQGISVLNVNDLANALKPTLLPGEAITVDILREGK
                     EPQQGVGYLDDGTMIVVEDGENYMGKRVEVVVTSMLQTSAGRMVFGRIRKEVRH"
     gene            28126..29274
                     /gene="ispF"
                     /locus_tag="P5541_02385"
     CDS             28126..29274
                     /gene="ispF"
                     /locus_tag="P5541_02385"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRV70852.1"
                     /translation="MKEWTCIIAAAGAGSRMGGVPKQLEILGDVPMWKWSLKTALSLA
                     EEGISEIIVVVPRDAVPLFDPGPVPVGVTCKVVEGGETRRDSVLSGLVSASREMVLVH
                     DAARPFASVDLFKRVMTGTCKNGATIPLVPVSDALKSVEGCRVKWLHERSAVRRTQTP
                     QAFPAVEAREVLSLAGRNVSDEAEAWLLEGKDLSWVEGELMNFKITYPDDMDMAQRIA
                     GSFFLKKTGIGYDIHPLAPGRPFILGGVHVPFPLGISGHSDGDLLCHALSDAILGASG
                     MPDIGTLFPAAEEKYKNASSISLLHEVLQRVHKEGISVESADAVITTQVPRLSEWIEV
                     ISRNVGKVLIEAGCDDFLLRAKSGEFTGPVGNGEAIACWAVASIRRKV"
     gene            29439..30914
                     /gene="guaB"
                     /locus_tag="P5541_02390"
     CDS             29439..30914
                     /gene="guaB"
                     /locus_tag="P5541_02390"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRV70853.1"
                     /translation="MENMKNRIIGDGFTFDDMLLVPSYSEVHPSQVEPGSFLTPGISL
                     NIPFCSAAMDTVTESRLAIALAREGGIGFIHRNMSAERQIAEVDKVKRSESGVIVDPF
                     FLHPEDLVKDAVDLMAHYHISGVPIVDEKRRLVGIITNRDLRFVTNYEQPISRVMTQE
                     HLVTAPVGTDLEGATRILMKHKIEKLPIVDDKGILKGLITIKDIQKARDFPKASKDSR
                     GRLRVGAALGVGEGAMERAAGLFEAGIDVLVVDTAHGHSRSVIEMVASIRKKFGNISL
                     VGGNIATGEAARALIDAGADAVKVGVGPGSICTTRVIAGIGVPQMSAVMDVAEVAHSM
                     GKKVIADGGIRYSGDVVKALAAGADSVMIGSLFAGTDESPGEVVIYRGRSFKSYRGMG
                     SLEAMKGGESRDRYFQEGQTDDKLVPEGIEGLVAYKGPMSAIVYQLVGGLRSGMGYVG
                     AKDIPELHENSRFVRITSAGVKESHPHDVVITKEAPNYRME"
     gene            31053..31580
                     /gene="rimP"
                     /locus_tag="P5541_02395"
     CDS             31053..31580
                     /gene="rimP"
                     /locus_tag="P5541_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRV70854.1"
                     /translation="MEKGSESKQLKDLHENFRDIVEGQGYEYVGSELVSEDGRTVLRV
                     LIDSLGGINVKDCEIVSRIIGKWLDDSDPGLPERYMLQVSSPGVERPLLRPGDFLRFK
                     GKKVRIRLKEAEKDKKTLVGVIENVAGEMIIIDDEESGNLEIHFENISKANLVFEMPG
                     KSKPGKKDRKRRGQQ"
     gene            31580..32737
                     /gene="nusA"
                     /locus_tag="P5541_02400"
     CDS             31580..32737
                     /gene="nusA"
                     /locus_tag="P5541_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRV70855.1"
                     /translation="MQLGRDFLRALKQITQERNLPLEVISSGLEAAMISAYRKYRGGD
                     HIVEVFVALESGEVSICEVRQVVEEVSNTDTELSLDMAREMGFEDVVPGDIIRVEVEP
                     EKFGRIAAQTARQVIIQRLKDAERQVIFEEFSDKMGDLVNGVIFKSENDQVLVRLNER
                     TEAILPREERIVGESYPLGSRFKFFLLEVRQTTRGPRIVVSRSHPGLLRRLLELEIPE
                     IREGTIEIREIVRESGARAKVAVSTLDTNVDPVGACIGSAGSRIKSISKELGGERIDV
                     VVWSSDPLIFIRNALSPARVVRVEPVLEQEKSVQVFVRPDHLSLAIGKAGQNVRLAAR
                     LTRWKIDIKVLEPEKLPTLQDLFEDLEIPEEDEKCKEEKMEQGNERDPEPA"
     gene            32683..32994
                     /locus_tag="P5541_02405"
     CDS             32683..32994
                     /locus_tag="P5541_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRV70856.1"
                     /translation="MQGRENGTGKRKRPRTCVGCGEEFGRKNLVRIVRTPSGDVAVDD
                     GGRSPGRGVYLCRKTDCVLKAKKRNALARSLRVSVPEEIWDIILELVEREESDQSGYQ
                     S"
     gene            32978..33340
                     /locus_tag="P5541_02410"
     CDS             32978..33340
                     /locus_tag="P5541_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70857.1"
                     /translation="MVIKAEILAEIRNLLGLTMKAGVLVRGQDSVRTELKKGASLLVL
                     LASDHSKNVASMVEGYRRRGQCEVLVFEGLERARMEKELVTGSTQILGLPRDHGLTIK
                     IRKLFVKGAVADEQDKNI"
     gene            33318..35273
                     /gene="infB"
                     /locus_tag="P5541_02415"
     CDS             33318..35273
                     /gene="infB"
                     /locus_tag="P5541_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRV70858.1"
                     /translation="MSKIRIYEMARMLEKSNKEMMAILGELGVKVNNHMSSIDSETAQ
                     MVEDAIKGAKASVEEKTGSPVSEIKVPAGVLISDVASIMGKKPAELVKVLVSSGYMIP
                     ADSPVNSDILEVLSIEFGMIITQDSGEEESKTAKTAKKAPLRGDHLVDRAPIITVMGH
                     VDHGKTTLLDFIRKTRITETEAGGITQHIGASAVVHNDRRIVFLDTPGHEAFTAMRAR
                     GAQATDIVILVVAADDGVKPQTLEALNHAKASGVPIVVAVNKIDKPGSRPDRVRQQLS
                     DHGLAPEEYGGDTVFVDVSAVTGHGIDQLLEMVLIVADMLEIKADPSVSPEGVVVEAK
                     LDKGKGPVATVLVQQGTLRKGDIIQTEDSWGKIRAMIDNTGKMVEEAGPSTAVEVLGY
                     SSVPQPGDIFHVVESEKEARQVFDEKQVSRREADTGPSRKITLEEMYDRMQTEEAPAL
                     SLVLKCDVQGSLEALKASLAKQGTSEVGINFIHAGVGRISESDVMLASASNAIIVGFN
                     VRPDPNAKKVAEDEGVQIRLYRIIYDVIEDVRAALEGMLAPTIREKALGQAEIRAVFK
                     IPRSGKVAGCYVTEGMMKRNAKVRLVRDGVVYWEGTLTTLKRFKEDAREVAMGYECGM
                     SFANQQDFEVDDIVEAFELVEEKRTLD"
     gene            35270..35602
                     /locus_tag="P5541_02420"
     CDS             35270..35602
                     /locus_tag="P5541_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRV70859.1"
                     /translation="MIPGDLFLFSGILFIQIKIPAAATLKDRRQITRSIQERAKARFN
                     ISVADLGPDGSCQDAFLVFETVSSSPDATEERILSVERMIRSMENKGDFYIVRGHREV
                     ESRAWFQD"
     gene            35583..35963
                     /gene="rbfA"
                     /locus_tag="P5541_02425"
     CDS             35583..35963
                     /gene="rbfA"
                     /locus_tag="P5541_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRV70860.1"
                     /translation="MPGFRIDRINRELLREISELIRTRIKDDTLAEAVLFDVDCSRDI
                     AHAKVFFRTIDPDRKDAVQKSLEKASGTLRGMLGRRMKLRKIPELRFVYDTNEDEALR
                     IEAILDGLDILVGDDERNTEESKD"
     gene            35960..36949
                     /locus_tag="P5541_02430"
     CDS             35960..36949
                     /locus_tag="P5541_02430"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRV70861.1"
                     /translation="MKTVNEIIRILLEADTWVIISHEKPDGDTLGSCSAFYQRGLSLG
                     KRCMWRGPDRIPVVYDFLPGSDKYDVPVPGFLREIRPGSVVIVLDTANQSRSIEGISG
                     LSKDIPLVNIDHHGDNKLFGTINWIDAKASSTGEMVFELFAIARWHPTPGEAESLLAA
                     IATDTGFFRFSCTSSKTLSAASSLVASGADISRIYGKIHENRSIHGLRLWGLGLKKAR
                     TLHSGRMGLTWLDRNDFKTTKADREECENLVNSLLTVSGVVLAVLLQEEEGYCRASIR
                     TREPVDARKFAEIWGGGGHLRASGCKIDGTLEEVLEEVGKKAGFLDEDGVSCS"
     gene            36924..37850
                     /gene="truB"
                     /locus_tag="P5541_02435"
     CDS             36924..37850
                     /gene="truB"
                     /locus_tag="P5541_02435"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRV70862.1"
                     /translation="MKTGFLVVDKNIGARSTSCVGTVKRILGKETRIGHGGTLDSTAC
                     GVLVLLAGKATRASRFVMDLPKLYLVTARLGIRTSTDDMSGEIISITDRVDVGDGQID
                     RAICSMMGLREQVPPSVSAVRVRGSRAHSIARKGLVPDISPRTVMISSIRRTGPVSEK
                     SEFSMEIRCHKGTYIRSIVRDLGEIFGCGAAVSFLQRVEVGPFKLGSAVPYEVLSISG
                     REIVEENLLPLSSICDWFTTYRMKGGNPASVLSGCPVPLDHLERTSWGKYQSRGIVVI
                     EGRGFVSFGSISVSGGVPAFSPSTTLESGDIY"
     gene            37852..38781
                     /gene="ribF"
                     /locus_tag="P5541_02440"
     CDS             37852..38781
                     /gene="ribF"
                     /locus_tag="P5541_02440"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRV70863.1"
                     /translation="MIGVLGSFDGFHLGHRKLFEIAGSLASELNDSWCVVTFFPHPQS
                     ILTKSPFSVLFSEPEKDILGFCLGVPEINRIPFTRECAELDPPSFLELLERNFFLRGL
                     VVGEDFHFGRERSGDASLLAELADSRGWITSIVESFIIDDRKVSSSSIRKRIEMGEIH
                     EAARELGYPFFLTGTVIHGEGRGRTIGFPTINIQLPFGKIRPARGVYSGCASIRGKSF
                     PAAVNIGSNPTFPGVREIRCEAHIPGYKGDLYGEDVSLFLFRRIRMEKTFRSPTELVE
                     QMGFDIRECFVDWEHMEPRMRIFMECCSSGIIS"
BASE COUNT         9599 a         8683 c        11079 g         9527 t
ORIGIN      
        1 ttcccgaaag gcaactcgcc tgaaagcgtt cgtttctccg cctgctacgg ggcgatcgca
       61 cagtgccggg aaaaggctcc ggcaggtgtg ctttccctgc ccctccgcga cggctgcgaa
      121 actcaccggc tcctgagcct tcggtaaagc ctccctccgg ccccggactt gttcttccag
      181 cctccggtct ccagattctt cgcctttccg agtcacgagt tgcgagtcac gagtcacggc
      241 acctgaacca gatccttcgc tgcgctcagg atgacataag gatcaagggc gtgaacggtg
      301 aacggtgtgc aaaggcaagg gcgtgagacg tgagacgtta gacggaaaaa gcgaggcaag
      361 agctttgtca ttctgaggaa tgaaatgacg aagcggaacc cgccagcagg cggacgttcc
      421 gcgccctttg cccaccggcg ggaatctggg tcagaaccag atccttcgct tcgctcagga
      481 tgacaggttt ttgatgtcat tctgaacccg aagggtgaag aatctggttt tgtgttttga
      541 acttcgcctg ctgcggggcg aggagcaagt tgccgttaaa aaggctccgg caacatcggg
      601 gctgccgatc gatggacaac atgagtgcaa ggaattagaa tatcatggca agcggaggcg
      661 agtgtgctct ggtgagtgcc ccgggcttca aacccggtgg gggtcggtac ggccggcccc
      721 ggtgggttcg atccccacac gtctccgcca tttttgtgca ccgatattcc cggcagatca
      781 ataaggaggt ctttctcttg caaaaaaaac gattcagtct taagggatat tcctatccac
      841 tcacaccggg gggaaagtcc tctgttgtag gagattttcc ctggcattat ggaactgaat
      901 acctaaatat ccttttccgg accgatccgg atgttatagc ttcgtacctg cctcatccgc
      961 tgaaaccagg gcctgaacct gatcttgcct atgtggcctt cagcagatgg tggtcagtat
     1021 gggaagacca gagggacatg gcctgcatca atccggaaag gacccagtac atggaagccg
     1081 ccatatggac gggttgttcc tttgaggaaa cagtgggcca gatatgcctt catatctggg
     1141 ttgataatga tttcagtatg gcaaggggct ggtttatggg attccccaag aagctgggtg
     1201 aagtttacat gacagattat caccctctta atcccgccat gcccaaaatg ggtatcggag
     1261 ccagattaaa aggaatagcc tccgcgcacg gagaaaggct ttttgaagga tccatggata
     1321 taacaggcaa atgttctccc ggggaactac cgcgtcccat gggtcttccc ctgcttcatg
     1381 taaggcattt cccttccatc gttaaagatg ctcccccctc ggtccttgaa ctggtctctt
     1441 tgggagcgga ggattaccgt tacggtgaaa atgtctggaa gggaaaggga agcctgaaat
     1501 tttatccctc cgagatagaa gagcattttc ctctggctcc gcgggaagtt cttggagcct
     1561 ggcattatac gtctggatac actttcccgg gcggtgaagt tcttttcagt tggctctgaa
     1621 aatagatatt caaaatggtt cccctcgtgt ataatgtggg ttgaaatata aggccgcttt
     1681 tcgataaatg ttagtgttat cgtggcggta tcttcaaggg atcagggggg ttactgagga
     1741 tatgaggaaa atactgcttg ctattgtctg catctcgctt gttatgggtg cttccgcttc
     1801 gtttgccatg gagcccgtag cagccaagga tctcaaggcc gggtttgttt acgtgggacc
     1861 tgtcggtgac ggaggatgga cctacatgca tgatatcggc cgtctggaaa tggaaaaggc
     1921 ctatcccgga atggaatcgt tctttgtcga gtctgtcccg gaaggccctg attctgtcag
     1981 ggttatggag accttcatca ggaacggtgc caatctcgtt tttgctacct ccttcggcta
     2041 tatggatttt gtccaggatg tggccgcgaa aaaccccgat gtggttttca tgcactgctc
     2101 cggttacaag actgctccca acgtcggaaa ctactttgga aggatgtacc aggcccgtta
     2161 tctctccggt cttgtggcgg gcaagatgtc caagagtaat gtaattggat ttgtcgctgc
     2221 caatcctatc cccgaagtta ttcgcgggat caatgctttt acccttggtg cccggaagac
     2281 caacccggac gtaaaggtca aggttgtctg gctcttctca tggtttgacc cggcgaagga
     2341 gaaggaagct acccttgccc ttattgatgc gggagccgat gttatagcca tgcacgcgga
     2401 ctcgggcgca gctcctcaga cagcggagga ggccggagtg tacgtagtgg gttacaacaa
     2461 cgatatgaaa agctatgctc ccacaaaaca cctgaccgct ccgatatgga actggggaat
     2521 ggtctacaag aagatagctg gagaagttat cgatggaaca tgggagtccc atgatatctg
     2581 gtggggcctc aaggaaggcc ttgtcggact tgcgccttta tctccggaaa ttcccgatga
     2641 tattgtcaag cttgtagagg ccgagaaagc gaaaatggtc gagggcaagt gggacgtttt
     2701 ctccggtcca gtcaagaacc agaagggcga agagatggtg aagaagggtg agaccatgtc
     2761 tgatggcgac atgctcggaa tgaactggtt cgtccttgga gtcgagggtg atattcccca
     2821 ataggaaatg gacttttgag ggggcgcttt tgcgcccccc ttttttttgt gatggtttag
     2881 aatcttgatt ggttcgtgaa taattagtgt cgggaggaag aatcggtgac acctactgca
     2941 gatgtcaacc tagcgtccat gcgggacata gtcaaatctt ttgtcggcgt caaggcgaac
     3001 gacgggatcg accttgatct tcaaagggga gaggttcaca ctcttttggg ggagaacgga
     3061 gccggcaaaa gcacactgat gaacattctc tgcggtcttt atacccccga gaagggtcgc
     3121 atccttatcc ggggggagga gatgacattc agatctcccc gggacgcgat agatgccggt
     3181 atcggtatgg tgcatcaaca cttcatgctt gttcccgccc agagcgtgtg ggaaaacgtc
     3241 gctctgggtc tcagggatct cccctttgtc cccgacaaaa acgaggttat cggcaggata
     3301 gaatctatcc gtgaaaaata cggcatccat gtagaccctc ttgccccggt atggcaacta
     3361 tccatagggg aacagcagag ggtggccctg atcaagatgc tgtatcgcga cgcggagatc
     3421 ctcatccttg acgaaccgac tgcggttttg actccccagg aatcaagggc tcttttttct
     3481 acggtcagac agatggtttc cgaaggtcac ggaattattt ttatttccca taaactggac
     3541 gaggtccttg aagtttccga caaaataact attcttcgaa aagggaaaaa agttgctact
     3601 gtacccgcct ccagggcttc aaaggaatct cttgcggaga tgatggtcgg gagaaaggtg
     3661 gtttttcaga tcggcaagac ccttcagaac cccggaagag tggttttgga aaccagggat
     3721 ctcttcgtca ggggtgaccg ggggcgaatg aaggtcagcg ggttcagtat taacgtaaga
     3781 aaaagggaga tcctgggtct tgccgggata tcgggtaacg gtcaggaaga actttgtgag
     3841 gccctagcgg gaatgcgaaa aaccgaaggc ggagggatcc ttgtcgatgg aacctccatg
     3901 gaggaaaggg aaacaaggga tttccagaga aggggcatag gatatatccc cgctgacaga
     3961 gtggggacgg ggctggttac aggcatgaat cttcgggaga atatcgccct taggaagtac
     4021 tggagaaaag aatttacccg gtttcggttc tttgtccatt gggaaaatat cgcaggcagg
     4081 accggtcaac ttgtggagag gttcggtgtg gtaaatcccg gactcggtta cccggccagg
     4141 atgctctcgg gcgggaacct tcagaaactg atgctggcca gggaattatc cgatgatcca
     4201 aaagttgtca tagcggtgca gccgacctgg ggacttgatg tcagtgccac ccaattcgtc
     4261 agggagacgc ttctggagca gagggacagg ggggcggcca ttcttctcgt ttcagatgac
     4321 cttgacgaga tactctcgat gagcgaccgg atagccgtta ttcatgacgg aaaactaatg
     4381 ggccttgtcg agcatcccga tacagtatcg gaggaacaga tggggctaat gatggccggg
     4441 tctccactgg aggaattcga agtaccccct gactccgaaa gggaatccac tggaaaggcg
     4501 gtaaccctcc aataatgaga tacaggatcg tcgtcaggag aagatattat acgagtcttg
     4561 ctttcgaaac cctgcttccc gtcctgtccg taatggttgc tcttttcctg gctgggttgg
     4621 ctctggcatt tttcggggtc tcccccgtca gggcatatat cgccatgttc cggggtgcac
     4681 ttggaagctg gtatgggatc ggtgaggttg tagtcaagag tattcccctg gttattgccg
     4741 gagttgctgt tgccctggct ttccggatga aaatctggaa tattggcgca gagggccaga
     4801 tttacgccgg ggctctcgcg gccacggctg cggtcagata cctgcagacc gacagccggt
     4861 ttgccatgct tgcagtcatg ctaatggctg cctttctggc aggaggttgc tggggtggag
     4921 ttgcaggtta tctgaaagca cgatggaatg tgaacgagat aataaccacc ctgatgttga
     4981 actatgttgc aatacacctg gtcgaatatt acatttacgg gccgtggaga gatccatcga
     5041 gcctcggttt tccgatgacc gctccttttc ccgcttccgc aaggctcccg cttttctttg
     5101 acagccgggt acatcttggg atctttgcgg ctatcatact tgttctggtt tacaggcttg
     5161 tcataagatg gacccgatgg ggcttcgaaa taagggccat aggagacaac cccaaagccg
     5221 cttcctatgc gggtatactt tccagtcgga acattattct tctccttttt gtctccggcg
     5281 ctgtcgccgg aatggccggc atgatcgaga tctccggact ccagggcagg ctgcagcacg
     5341 ggttctctcc cggttacggc tacacagcaa tcattgttgc gtggcttgcc aggcttaacc
     5401 cctgggggat catgatcgta tccttcttta tgggagtcct cctggtggga ggggacacac
     5461 tgcaggtggt aatgagactt cctgtcgcga gtgttcaggt cattcagggc tgtattcttt
     5521 ttgccgttct tgcgtcggag ttcgccagaa actacaagat acaggtgaca aggaaggagc
     5581 gtgagtaggg tggatattct tatccctatt cttgcggcgg cagtaagaag cggaactccc
     5641 atactttacg ctactcttgg agagattctt accgaaaggt cgggggtcct caacctggga
     5701 gtcgagggac ttatgcttat aggtgccttt tccggcttta tggcggccca cgccagcgga
     5761 aatccctggg caggagtagc cgtggcattt ctggcggggg cttgcgtatc ccttgtgcat
     5821 gcttttttat gcgtcaccgt aggggccaac caggtcgtta gcggtcttgc cgtgacgatg
     5881 ttcggaacag gggccagttc cctgttggga aggaaaatga taggcgagac catcgcgggg
     5941 ttcgggaagt tgcagatccc ttttctttcg ggcatacctg ttgtggggcc agtattattc
     6001 aatcatgatc ctctggtcta cgtttcttac ggactggtga tatttctggt ctggtacata
     6061 tttcacaccc gcccggggct taacctcagg gctgtgggag aatcccccaa agttgtggat
     6121 gccatgggtc tcagcgctgc aaggctcagg tacttttaca ctgttttggg agggggtctg
     6181 gcagcagtag gaggagctta catgtctctg gcctatacaa aaatgtgggg agaggggatg
     6241 accgcaggaa gaggatggat agctgtagcg ctggtcatct ttgcgatatg gcaccctgcc
     6301 agggccgttc ttggagccta cctgttcggt ggggtcgggg ccatgcaact ccggatacag
     6361 gcggcaggga cgacatttcc cgctccgctt ctcatgatgt tgccatattt gctcaccata
     6421 gcagtccttc ttttgatctc cataagaaag ggaaaaggta ttcttctcgg tgctccagcc
     6481 gctctggggg ttccatattc cagggaggag agaggatagg aacctccaat ggataaatcc
     6541 ccctgctcta ggctccgcgt ctggatatat ccgctattgg ttcgatgtac tggtattgag
     6601 gttccgaatc ccaggggaaa aggatccagg tttcctggct gacttccgtg ataaaagtgt
     6661 cagtcagggg tctcccgtcc ggctttgcat aaaccgtcgc aaagtgactc atgggaagca
     6721 tgttccttac tattctggct gttttgcctg tgtccacgag gtcgtctatg atcaggcact
     6781 tttcactctc tccgaaagtt ttcttgatca ctttcgattc tccctgggaa cgaactgtgt
     6841 agcttgatac gcatattgtg tcaatattgt gaatgtcgag ttcccgcgca attattgccg
     6901 cgggaacaag gcccccgcgg gcaacggcga tgatcttatc ccaatctttt ctgatctcca
     6961 gaaggcgcca ggccaaggct ctgcagtcat tgtgaagctg cttccatgaa acggggtagg
     7021 ttttccggta tttttgatct gttccgctca tgttcttcct cccggagggt ttttcatttt
     7081 gtgatccttt tccatcttgc tccctgggga gtatcctcaa ttgcgatacc cctggcggag
     7141 agttcttgcc tgattttgtc tgctgccgcg aaatccctgt tttttcgagc tatttctctg
     7201 gctgcgacca tttcatctat ttccgggtcg cccggttcgg gggatccttt tttcaggtac
     7261 tcaaggccta taatacccag aaccttgtcg caatgttcca gaaaagaaat ggcattgttg
     7321 aaaaaaaccg gggatacagc ctggccatga gcgactgcca gattgatcgt cctgatcact
     7381 tcaaaaagag ttcctatcgc tccggccgtg ttgaaatcgt cgtcaagtga tgaattgaaa
     7441 ctggaaacgg actctacgag ggaattttct ccgttaatgt tcgaggttgc tttatccgtt
     7501 acgggtgcgg aaagaagatc ggtaacgctg ttttttattc tgctgatgcc gctctcggcc
     7561 tgcttcaggc tctcatcgga aaaacttatc ggggaacggt agtgagctcc aagcatgaat
     7621 agccttatgg cgtcgggtga aaacctttcc cgcgcttccc tggctgtgag aaaatttccc
     7681 agagacttgg acattttttc ctggtccaga aggatataac cgttgtggat ccagtatctg
     7741 acgaagggtt tgtccgatgc cgcctcggcc tgggcgatct cgttctcgtg atgggggaat
     7801 acaaggtcgc ttccaccagc gtggatgtca atggtttcac cgagaagttt catggacatc
     7861 gcgctgcatt cgatatgcca tcccgggcgt ccctcgcccc aggggcttgt ccaggagggt
     7921 tccccctttt tttgggcttt ccacagggca aaatcgagtg gatgtctttt tctggggtct
     7981 acatcaacac gtgctcccga cagaaggtcg tcgagggact gccgtgccag ctttccgtat
     8041 tcgggaaaag tttggacctc aaaataaacg tctccgtcaa cgacgtaggc gtttcctttt
     8101 tcgacaagtt tttcgacgag agcgattatc tccggaatga attccgtggc cattggagag
     8161 aaggtcgggc gttttattcc cagtgcgtcg gcatcttcat aataggccgc tatgaatctt
     8221 gaagccagtt cgggcactgt catttccaac tgggcagctc tggctatcat cttgtcgtca
     8281 atgtctgtga agttctgggc gtaaagaacc tcgtacccgc aggactcgag gtaacgtctg
     8341 agagaatcaa agactatgaa tggccgggca ttgcctatgt gaaagtaatc atagacagtc
     8401 ggtccgcagg aatagaaccg gacttttccg gattccagtg gttcgaatat ttcctttttt
     8461 cttgtaaggt cgttgaaaag cctcaatgtc atcactgcac ctccagggaa gcggctgttc
     8521 tcttactgag gtatcctata ccaagaggtt gcggagatac aacctttgga acgcatttaa
     8581 ccgctgtccg ggaactttca ataattgacg cgaaggaata gactatttaa gtcagggaag
     8641 gaacaaaccg cgaggagaaa agaaaatggc tgctgtggat ctttcacgca aaatatccga
     8701 actgcccctt cggccgggag tttacacttt tctggacgag aaatcagagg ttatatacat
     8761 aggaaaggca aaatctctca gaaaaagggt tcagtcctat ttcaggcaca gcggatttgc
     8821 ttctccaagg ctcagaaagc ttgttgaatc tgtctctgac gtgtcggtaa taaggaccga
     8881 gagcgaggcc gaagcgctta tcgttgaagc acgcctgata aaacagtatc agcccttttt
     8941 taatgtggac ctgaaaatga acgagagata cccctatgtt aaagttacct ccgagcccta
     9001 cccgagaata gtgattacta gaaccaggga taaggacggg tccgtatata ttggtccttt
     9061 cgtcagcgtc agggaactca ggcagatgtt ccggatgatc gagagatatt tccctctcag
     9121 gaactgtaaa agtgattcga gtcgaggaag ctggagagac aggccttgcg ttaggtacga
     9181 acttggaaag tgccccgccc cgtgtgttgg tacaggaaat ccttcggaat atcgtgagag
     9241 ggttgccgat gttgttcttc ttctcaaggg gcagtcggcg gaacttgtcg gaaggctcag
     9301 ggataggatg gacaaggcat ctgaaaatct ggatttcgag accgcagcca gattgaggga
     9361 taccatacgg tcattgtgga aaatgtcaag gcagcacgtt tcgctggctc tttccgagcc
     9421 cgccgaaagg gaggtctgga agggactcct tggtctgcag aaggttcttt ccctgccatc
     9481 ggttccctgg aggatcgaag gatatgatat ttcacacttt gctggaggag agagttacgg
     9541 ggtatgtgta gttttcgaac agggaacgcc gaacccggat ctatacagga agttccggat
     9601 aaaaaccgtg gaaggagtgg acgatttccg cgctgtggca gaaactgtcg gaagacgttt
     9661 ctccaggctc aaaagcgagt cggggtctct tccgcaactt gtaattatcg atgggggaga
     9721 gcaacagcta aaattcgtga ctgaaagact gaaggaactc ggaatggaag atatcccttg
     9781 cgttgccctg gcgaaaagag aggaactcct ttttctcccc gatagaactg ttcccctggc
     9841 gcttcccaat ggcagtatgg aattgaccat tcttcaaaga gtccggaacg agtcccaccg
     9901 tttcgcgatc acggccagca ggaatgccgg gagaaaaaga ctttcgcggt ccgctctcga
     9961 ggatattccc ggcatcggaa agatacgggc cgcccggctt ctgtcccagg tcggaagcat
    10021 ggaaaggatc gcttcccttg atcccgagga tctttccagg gtcgagggga tcggcgaaaa
    10081 aagggcacga atgatcctca tgcatctgaa gggagtcgtc gaaacaaatg acaatggctg
    10141 atcgtcaaat agacgaattc tatatgagaa tggccttgag tctcgcaatc aggggcacag
    10201 gagggacaag ccccaatcct cttgtaggca gtgtgattgt tgatgagtca gggatatgcg
    10261 ggtgggggta ccattctgcc cccggttacc ctcacgctga ggctgaagcc ctctcgatgg
    10321 ccgggaaaaa agcggctgga gccacccttt atgtcaacct ggaaccctgc tctcattttg
    10381 gtaaaacacc tccctgtgct ccccggatca tagaggcggg gataaaaagg gtcgttgccg
    10441 gtataggaga cccagatcca agagtttctg gcaggggttt taagatgctg tcctccgccg
    10501 gaatagatgt cagagaaggc gtgctggaaa aagaatgccg aaagatcaac agaggttttc
    10561 tgtccagggt cgaaagaaat cgtccctggg taacgctcaa ggcagctgtt acccttgatg
    10621 gcacaatggc tttgggatca ggggaaagca aatggatcac caatgaacaa tcaagggcca
    10681 gggcacacct cttgaggggg tgcaatgatg ccgttcttgt cgggagaggg acagtggagt
    10741 gtgatgatcc tctccttaca gtaagaaagg tttctggcag ggatcctgtt gccgttgtgc
    10801 tggatccggg cctcagtatg tcttcggggt caaatcttgc cagggaggga accatcgtgt
    10861 tcggtctcgc gggggctggc ctgggaaaac gcagaagcct tgagaggcaa gggtgtcgag
    10921 tgtttctggt tgaaagcgac gaaaagaaca ggatacccat tatcccgatg cttgaggttc
    10981 tgggcagcaa agggatcaac aacctcctgg tggagggagg tccgctggtt gccggatcat
    11041 tcctggaaaa aagatgtttt gattccatct ctttctttgt ttctccgcga attgccggag
    11101 aggggcgaaa gatcggagaa gggttcataa tatcggggtt cgacgtctcg gctgaggtca
    11161 gtataacctc ggcagtcgat gtggaaggag atctctggat cgagggggag atgagatgtt
    11221 caccggattg atagaatgca ccggagtgat agcttcctcc aggcagcggg gggatgttac
    11281 ggaaatcgac atcagggacg ttccctttgc cacctctctt gaaaagggtg attccgttgc
    11341 cgtgtccgga gcctgcctta ctgtcacaag gatcagcggg accgggtttt ctgttgagat
    11401 gatgcccgag acccgcgtaa atacaatacc agggctgacg agccagggat acagggtcaa
    11461 tctcgaaaga tccctaagtt cttcaggaag gttcgaaggg catattgtca ccggacacgt
    11521 ggatactgtc gccagggttt cggggatcac ggaactggga agaacccgga tcctttcgat
    11581 cgatgcagga aaaaagtaca tgaaatatgt cccgaggaag ggatcaatag cgatccaggg
    11641 agtaagcctt accgtgatcg attccagtga tggtatcatc cggacaggtc ttattccgga
    11701 aaccctttcc agaactaccc ttggtgatct tgagccgggt tcagccgtta atctcgagtt
    11761 tgatatactg tgcaaataca ttgaggggct tctttcagga aatggtaccg aacccgaaaa
    11821 gggaagccgg ataaacctgg attacctcgg tgaaataggc tgggcatagg aacaggcagg
    11881 aggtaaaaaa attggagggc aatacaggag aaaagttttt ctgtacagcc gaagaggcta
    11941 tcgaggctat cggcaggggt gatatggtaa tagtggtcga cgatgaatcc cgcgagaacg
    12001 agggtgacct cgtaatggcg gcagaaatgg tcaacaggga gagcatcaat tttatggcct
    12061 ccatggcaag gggtctgatc tgtgtacccg tagatgctga agtagcaggc aggcttggcc
    12121 tggagccaat ggtccaggat aataccgata ggcactcaac ggcctttaca gtttcggttg
    12181 atgccaggga aggcactaca acgggcatct ccgctgctga aagggctatc acagcgaggc
    12241 ttattgccga tcctgcggct acggcggaca agttcagaag acctgggcat atgtttcctc
    12301 tcatcgcccg taacggggga gttctcaaga gggcaggcca caccgaggca gcagttgacc
    12361 ttgccaggat ggcaggaaaa actccggcag gtgtaatatg cgagatcatg aacgaggatg
    12421 gttcaatggc aagacttcca gaccttgtgg attacgcaag caagcataat ctcatgatca
    12481 tttccattga ggacctcatt cgttatcgca acagaaagga caggcttgtc gaaaaagtcg
    12541 ccgttgttca tcttccgacg gaatatggcg attttatggc ccatgcatac cggtttactc
    12601 ttgatgaaca gaaggacagg gtacatcttg ctcttgtcaa gggaaacctt gccagagacg
    12661 aagcgactct tgtcagggtc cattccgagt gtcttaccgg tgatgccctt ggttccctga
    12721 ggtgcgattg cggacctcag cttcattctg ccctgcggat gatagaagag gaaggaagcg
    12781 gggttcttct ttacatgaga caggagggtc gcgggatagg cctgctggag aagatgcatg
    12841 cctacgaact gcaggaaaag gggctcgata ccgttgaggc gaacgaggct ctcggtttca
    12901 agcccgatct tcgtgattac ggaataggcg cacagatact tgctgacctc gggttgtcaa
    12961 ggataaggct gattaccaac aatccgagaa aaataatcgg acttgaagga tatggtcttg
    13021 aggtttcaga gagagtcccg gtagtaatat caccgaactg ttacaacgaa aaataccttc
    13081 agaccaagga aaacaaaatg gggcacatgc tccatctcag ggacatcatt gggtaggtaa
    13141 aaatcatttt tgggggaagg gatagaagat gaagatcatt caggggaaac tggtcgggaa
    13201 aggacagcgt tttattattg ccgtatccag gttcaacgaa ttaatctcct ccaggcttct
    13261 tgagggggcg aaagactttc ttttgaggca tgatgtctct cccggggaca ttgatgtggt
    13321 ttgggtacca ggatcctggg aacttcccct tgtagccagg gaatttgccc tgaccgggaa
    13381 atatgatgcc gtgattgccc tgggggctat catacgcggg gatacccctc actttgatta
    13441 tgtgtcggcg gaggtctcaa aggggctggc catggtcggg atggagcaca gggttccggt
    13501 ctcgttcggg gttctgacat gtgacaacct cgaacaggct ttgctgcgtg ccggcagcaa
    13561 gtccggaaac aaggggtccg acgctgctgc tgccgctgtt gagatggctg atcttatgaa
    13621 gcagatacgg tcagtgaaat atggagaaga aaaagatgct tgatataaaa cttttcaggg
    13681 aaaaccccga agacattaaa agtttactca gttccagaaa cggcaagttt cccgtggacg
    13741 agatcgtcag cctcgactcc gaaagaagag aaatactgac aaaaacagac gacctgaagg
    13801 cagagagaaa caggggttca agggaagttg cagagatcaa aagaagcggt ggtgatgccg
    13861 cttccatcat ggaaaaaatg aaatcactgg gggacaggat cagggagaac gacgcaaaga
    13921 tccttgaact ggattcccgt atccagtcca tgctactgga aatacccaac cttccccact
    13981 catcagtccc cgcaggggag gatgaatcgg ccaacgagga gatccactcg ttcggcgaga
    14041 taccggtgtt tgaatttgat ccacgtcctc actgggagat aggagaggaa cttgggataa
    14101 tggatttcga gaggggcgtg cgtctcgccg aaagccggtt cacggttctc aagggccttg
    14161 gggcaaggct tgaaagggcc ctgatcaatt ttatgctcga cctgcacacc aatgatcacg
    14221 gctttattga gatcgaacct ccctttctcg tcaacagcga taccatgagg ggcacgggcc
    14281 agttacctaa gtttgccgag gatctttatt cctgttcctg cgacgatcta tggctgatac
    14341 ccacagccga ggttccactc accaaccttc accgggacga gataattccc gaggatgaac
    14401 tccctgttta ctacacggcc tatactccat gtttcaggag agaggcgggg agccacgggc
    14461 gagacgtcag aggcatgctc cgccagcacc agttcgacaa ggttgaactg gtaaagttat
    14521 gtacaccgga aaacagtttt gatgaactgg aacttctgct cagggccgcc gaaaaggtcc
    14581 ttgagaaact ggagatacca tacaggacgg tggtcctttc cagcggagac atggggttct
    14641 ctgccagcaa aacctacgat atcgaagtgt ggctgccgtc acagaacaaa tacagggaaa
    14701 taagttcatg cagcaactgt accgatttcc aggcccggag aatgaataca aggtatcgct
    14761 ccagaaacgg aggaaaggtc cagtttgtcc acactctcaa cggatccggg atcgctgtgg
    14821 gccgttgtct gatcgcgata ctcgagaact tccagcaaaa ggacggatca gtgttgatcc
    14881 cggaggcgct tgtgccctat atgggaggag tccgttcctt ggaaaatcct cgagaacaat
    14941 gattatttac ccgttttcat gataaaatca ccttattgca gatcgaattt ttctccatgc
    15001 ggggagggcc gttgatgcct gtggatgtac gggagatcca ggctacgctg tttcttgcac
    15061 tttccctttt cgtatttttt ttccttttgc agaggatctc caagagatca cttgagaggg
    15121 atcaatacca gttcacaaaa gatgtgggcc tccttgccgc atgggcgatc tgcggtatct
    15181 gggcatcgga tccttccgtt cgccttgttg tggcgctggg gctcgtttcg gcagtgatgg
    15241 gagtgggaca gagagtagct ccccgtgtgc cctgggcagc tggatttttc gctgtcggcg
    15301 ctgtccttgc ttcggcgggt atgagaatat cattcatagg tatcccgggc ggtgattatc
    15361 ttttccttaa cgattattct tcgctgcttg tcacaacgat ctgggtgggg atgtttcctc
    15421 tcttgctaca ggaaatggac cagatccctg ggcttgctgg ttttctttct gcgatcacct
    15481 gggtgatcat gcttatcgta acaagccttt ccaggcaaca tatatctggc gcgttctaca
    15541 tgacccttat gggagtcgtt atactggccg ttttctggag tcggcacgga cactcctaca
    15601 gacgcctcgg agaacctctt gccgctctct ggggtaccct tatagccgga acctccatta
    15661 ttggcgtaag caagggggtt accttttcaa cactgatgct tattcccctt ggcctctttg
    15721 ccatccctat agcggaagct tccttcaaca ttgtcagcag ggctttcagc tcccgtcccc
    15781 agggcactct tttcctttac agaagacttg tcaacagggg tcttgatcat actcccgcag
    15841 tcaggatggt aggggttctt tgcgctgtgt tgggagcggt ggtggcatcg gtacagttga
    15901 gggacgagaa aactgccctt gctgcctttg cggcatcagc tacagtaatg gcgggctccc
    15961 ttcttgtgat gtacggggga tctcgcgggc ggaacgttcc tgtagccaga acaggaatct
    16021 ggggagtccc cgtggacagc atttcagtag attatgccct gggtaaggtc cggtcatggg
    16081 taaacctgga tcatgggggg agatacattg tcacccttga tgctctgtct gctctcaggg
    16141 caagagagga taaaaaattc cgggatcttg taaggggtgc cgatcttgtc ctgcctgatg
    16201 ggaaagggtt gattgcagcc ctgtcttttc ttggaacccc ggtggcacag cgtatccctg
    16261 gtgtcgagtt tgtcgaaaaa atgtgtcgtc ttgcctcgac tgaaggctgg cccgtttttt
    16321 tcctcggcgg ggcggaaggg gtagcagcta aggcggccct tattctggaa agaaaatatc
    16381 cgggccttgt cgtaggagga atcagccacg gatatgtcga atccggagat atgccatctg
    16441 tcgttgataa tatcaaaagt tcaggctcaa ggttcatttt tgtcggtatg ggtgttccct
    16501 gtcaggagaa atggatgtcg gaatatggca agactattga caggagtgta agtatcggcg
    16561 taggaggcag tttcgacgtt atttcgggta acctcaagag agcaccttcc gctgtccaga
    16621 atatcggttt tgaatggctt tacagggtga ttcaggaacc gtggcgctgg aagcgagtga
    16681 aacgccttcc ggagtttgtt atcaaagttt ttctcacaag gatcggtttt tcctctctga
    16741 agggagatta ggcttttgac agtgaacgcg gaacatttga tgcacaggga tctttcggca
    16801 gtttccgagg atgatctgat tcttgatgcg gtgcacctgc tttacagcca gaggctaagc
    16861 ggtgttccgg tcatcaggga tgactgggtc ctcaccggct atctttctga aaaagacatt
    16921 cttaaaggcg ccacgccgac atatcttgag attttggccc agagttcctt tcttgacgat
    16981 tccgaaggtg accttatcca gagactcagg cagatcggag accttccagt aagggacctg
    17041 atgaacagag atcccgtctt tgtgaggcct tccgagagcc tgatgaccgt tgttgacctc
    17101 atgctgcgaa aaggtctcaa aagactccca gtggttgatg gcaggaaact tgttggtgtt
    17161 atcgaccgtg gagccttttg tgagttcatg atggagggac agaacttcaa tgattcaaga
    17221 taaccttgaa gcgaagaatc ccgatgagag tggagcttgc cttgatgagt tccggcgcga
    17281 gcttgaagtc agactcgaga tcgaaaaaac gaagatgaaa aaggagcagc aggaagccct
    17341 tgcccgaata gaacagattt ccctgaactc cataaaacag gccgaggccg aatgggaaaa
    17401 ttacgagaca cttctggaat ccgcaaggag cgagctttac aggagatcca gggatgagat
    17461 gtcccggctt ttttctgatt cttgctctct ttccggcgag gaagattttg taacccgggt
    17521 aattgcaaaa atactcccct ttgatgaccc ggaaaaaaac aacaatggtg gttcaggaaa
    17581 ttgatcaggg acatggtttc catgaccctg cgggggcttt tagccgaccg ttcaatggtg
    17641 atcgagactc ttcacgatga aggtgtactt catttccagg aagaaacact ggaggtggag
    17701 accggagaaa gcgctgcagg actcaagatt ctcagggcca aagctctggg ccttctcgaa
    17761 gcacttgagt ggcgagagtg ggcggatatt acctccctta acttgcagga gaaaagaaaa
    17821 gccctgtcgt ccccggacag tacaataatt gacggcatcg gggaaagtct cgattcattc
    17881 agctccaggc tgaacgaact gaaaacagtt cgttctgaac ttgagggaag gatgaattcc
    17941 attcgcggta cgcttaagac acttgagcat tttcggtcct tcatcaaaga caatatcgaa
    18001 aagggaaaca gtatatcgat atggtggaca cagcgtggca atgtggctga aatggtttca
    18061 atggtacaga aaaggataca taaccgggat ccccttggat tcagtgacag tgtgacctac
    18121 cacacagctg tagcggctgg gggcgaagct gttgccgcaa taggcgttca ccccgataac
    18181 atcgaggatg tcaggggggt aatgacggaa aacgattgcc ttgcctggca gtttccctcc
    18241 gaatttgagg gttttcgtta cgacgaggct atcagacaga tggagatagt tcagaaggat
    18301 attccccgga ggatccagga gacagaaaag aaccttcaaa acaccaaaaa tacatggggt
    18361 ccaaggattg gcgcgctgta cgttattctc gatgaaaaag ttcaggagat cttcgtagag
    18421 catggaagcg agacaagcgg ggagattttc aagctggagg gttgggttcc cctggacgaa
    18481 aaagatagat tgaccacgtc cctgcgggaa aaatttgacg gaaggatcct gctttcctgg
    18541 aaggaaccct cacccgggga atgggcaaat gtaccgacct ccctgaaaaa tccccggtgg
    18601 ttcaagcctt tcgagatttt tctggctctc atgcccgtca tagggtacag ggggcttgat
    18661 cccacgatag ttatcggggt ttttttccct tttttcagcg ggtgcatgat tggagacgcc
    18721 ggatacggtg ctgtgatcct ttttcttgcg gcatggcttc gcagaatgaa gacaaggccc
    18781 gttgcggtgg atatcggatc catccttctt ttcgtcggtt catggtctat tgcctgggga
    18841 attgcctatg gagagttttt cggagacctt ggacacaggc ttttccacat ggaacccctc
    18901 tggctggaaa ggtcccaggt ggtgatcccc gtaatggttt ttacagtcag tctgggtttc
    18961 gcccatgtgt tgcttggctt tgtgctgggt gttgttcagg gtatcaagac agggcagaaa
    19021 catctttggg tagaaagact gggaaatatt ttaatccttg cggctctttt aagcgccatg
    19081 attgtggtca aggggtggct tcccggaaag atgttctctc tcactgttac ctttctgctt
    19141 ttgggcctgg cccttcttct cgcgggtgga ggtatgggtg gactggtaga gtctcttggg
    19201 tcaataggta atattctcag ttatgttcgt attgccgcta taggtctttc atcggcaatt
    19261 ctggcaatgg tcgccaccag tttcgtggat tccatgggtg tctcctttgt cggtgttttt
    19321 cttgccctga cgatccacct tctgaatttt gttcttgccg tagcgggatc gggacttcat
    19381 tcggccaggc tgcaatacgt ggaatttctt ggcaaatttt atactggcgg aggaacaaga
    19441 tataaacctt tctcaaggag gaaacccgaa ctatggaaaa agcgctgatc gcctttgcgg
    19501 cggctcttgc ggtaggtatt cccgctatcg ctacggctta cgcacaggca aggataggaa
    19561 gtgttggggc aggaaccatt gcggagaaac ccgaaaccgg gggtatcatc attatcctgg
    19621 aagccattcc cgaaactatg gttattctcg ggttcgttgt tgctgtaatg cttattctgc
    19681 agtttgcctg atagggaaat atggaactga atcaggaagc tggagcagtt ttaaaaggtc
    19741 cctgtaagga agatgaaaca gaaaaattca ggcaggctct tgaaagtgaa tatagcaaga
    19801 ggttatctga attgaggctg gaatccggga gatttatctc cgagaggatc tttcttgttc
    19861 gcagggaaac agaaaaacgg gtacaccata ttcgagaacg ccagaggaaa caatacgaga
    19921 ccactctgga gggggagaaa ctccgtgttc ttctggatgt gcgtatgtcc gctcttcagg
    19981 agatagcgga ccttttcgaa gcaatttctc tgaagataga aaaggagctt ttgctattga
    20041 gggaaaacca ttccggctat ccgaaaattc tggaaaacct gttttacgaa gccctggaaa
    20101 ggttggggga atcagccgtg gttatagttg ctcccggtga cagggccttg atcccccagg
    20161 atccgctagt tagttcggta gaagagcaag agtccttgct cgattgcgga ggttgtgtcg
    20221 tcgttgatgc tcagacaagg acgatagtta tagacaacag cgtaagaacg cgatggaatt
    20281 cgctaaaaag ggaattcatg cgcgaatttt cagggaagta tgcagatgtt cttcagggat
    20341 tcgacagatt ttcccgaaaa ctacggatat cttaacgccc gtctgagggc tcatttttcc
    20401 gatttcctcg gagaggacga ttatcggaaa cttgccggga agtcacttca gggtatagag
    20461 cttttccttc ttgagaccag gtacgggaaa agtttcagaa aaggacttgt tgaacagccc
    20521 ctttcctccc acggcaggat tgagagggcc ctggccgata catcagccga agtgatgaat
    20581 cataccagaa atatggcaat gggagaaccg gaggacctta tgcgcgtcct tctggcccgg
    20641 gctgatcttc ataattgccg gctgcttctt cgtttctttt ttacgggtgc gatttccgga
    20701 ggcgcacccc tttggcacgg ttacgggacg ctcccggtgt ccttcttcga gaggctgtgg
    20761 aaaagcagat ccataacgca tgtgatcgat aagtgccttg cctttcctca cccaatgtca
    20821 aggtcgctgg gaaaagcctg ctcggaactt cagaagggct ccccacttcc cgttagcgaa
    20881 cgagtccttt tagtggagat cgttgatttt ttccgccaga taattgaaaa acacaggacc
    20941 aagaacggag atgttgtccg ggaattcctc ggaaggacaa ttgatatctg gaatattaac
    21001 atctggtcga gagctatctc gggtactatg aaaatcgaaa aagcaaaagg gctttatatt
    21061 tccggagggc gattccttgc cgctgacaaa cttgagaatt cttctggacc ggaagaactt
    21121 ttcaggtcta ccccctggga atcgatgctt tcaggttcac gtggacgtag tgtcggtttt
    21181 gttgtgagca gggcgaccag gattttctgg aactggcaga tgtccctcag aagggtcgat
    21241 ccgctgggga ttgaagtggc cgtggccttc attgcgtgtc agcttgtcga gtggcataac
    21301 cttaatgctc ttgtagtggg gatagatatg ggcctttctg tggagtctat catggataaa
    21361 atgatcctcc aggggtgacg taaaagaatg aaggagagcg aaggaagagg atacgtcctc
    21421 ggaaggcaac tttttatcga gctctggtcc ctcctgggta tggaatcgat ggtatgtgag
    21481 acagtgtcag aattccccgc gtgttttgaa aggctccagt cggagagcat agcctttgtt
    21541 cttgtcgagt cagactggtt tgagaacatt ccagagatct acaaaagaaa gatcaggaca
    21601 ggggaaagcc cggtctgggt cgagatgcct tctttaaaaa gcagcgtaaa aagttgggag
    21661 tgaggcaatg tcaacccttg gatccattaa aagccttgcc ggaacagtaa taagggcaag
    21721 cctttcttcc ccggtgacca attttgaggt ggctcgggtc ggtaaagaga ggctcttggg
    21781 agaggtcata aggatagatg gtaacgaggc cgacatacag gtttacgagg agatcggtgg
    21841 tgtccatgtt ggagaacccg ttgagtttac gggtgaactt ctgggcatag accttggacc
    21901 tggcctactt ggatcggtac ttgacggaat tggtcgcccc cttgaatttt ccgaagaagg
    21961 tggagctcca cccgtctttc tgaaaagagg ctactatgta aacagtcttg accagtgtaa
    22021 gaaatgggaa ttcaatcctg ccgtcgccga aggagacact gtttctccgg gggatatcat
    22081 tggtcatgtt gaggagggga agggttttct tcatgccgtg atggtcccgc cgttcacgag
    22141 gccttccagg gtcggtatgg tgcttccacc cggttcattg gttaccgtca gggacagtgt
    22201 atgcgaactg gaaaatggtg aaaccatcgg actctctcag cgatggcatg tcagaaggcc
    22261 aaggcctgtt ctggaaaaac ttccctttac ggaacctctt gtgacaggtc agagagtact
    22321 ggataccctt ttcccggtcg ctctgggagg agccgcggtt gttccgggag gttttggaac
    22381 aggaaagacg gttacacagc aatctctggc caagtggtgt aatgcccgga tcgtaatata
    22441 catagggtgt ggggaaagag ggaacgagat gaccgaagtc atggaggaat ttccccagat
    22501 caccgaccct cactccggca gaccgctcat ggaacgaatg gttcttgttg ccaatacctc
    22561 aaatatgccc gtggccgcaa gagaggcaag catatatctt ggaatgacta tcgcggaata
    22621 ctatcgggac atggggctta gcgttgctct tatggcggat tccacctcac ggtgggcaga
    22681 ggccctgcga gagatctcgg gtcggcttgg agagatgccc ggagaagacg gatatccggc
    22741 atacctgggt tccaggcttg cccagtatta cgagagggct ggcagggcaa gtcttttggg
    22801 gactccgggc agggaaggct ccataacgat attcaacgca gtatctccgg gaggagggga
    22861 cttttcggag cctgtaaccc agtcaagcct tcgtctttcc ggggctttct gggcactgga
    22921 caagagactg gccgaagcga ggcatttccc ctccataaac tggaacagga gctattccct
    22981 gtatgaatcg ggactacgta ctgttttcga gaaaaggctc ggtaagcagt ggagtgttgg
    23041 ggcttccttt ctcaaggccc ttctggacag ggaagccgaa cttttgtctc ttgtacagat
    23101 agtcggaaga gatgccctgt cagaggagga caagtggcag atcgagatgg gtgagatagt
    23161 gagggtaggg ttcctgcagc agaacgcctt cgatcccctg gatgctttct gttctacgga
    23221 gaaacagatg ggtttcacaa aaacgttaat ggaccttaat gcacttgtct ccgagagact
    23281 gggcaagggg gtcttccatt accagattca ggaactgggg ttggtcagga aggttctttc
    23341 cctgagaaaa gtgggagaaa aagaattccc ggctgcttct tccaaatggc tggacgaggt
    23401 agaaagatcc cttgagaaag ttgagtccat agctggatga gatactacag ggagggaaca
    23461 aggcttgtag agggtatttc cggtcctctg atatttgttg gtaacgcaag gaacgctgga
    23521 cttggtgaag tcgttacaat agagaccacc aggggcaata ggactggaca ggttctccag
    23581 gtagatggtg acgtatgcgc aatacaggtg ctcgagggaa cattgggcct gaatccgtcg
    23641 gatacaaccg tctggttcga gagggacgtt gtaaaagtca ggctcagcga ggcgctaaag
    23701 gggcagatac tcgacgggag cggggtcccc cgtagcgggg ttcctattcc ctattttgac
    23761 aaagaagaac ccatcaccgg atctccgctt aatccggcga tgagatcgag tccgaatgct
    23821 tttattgaga cggggatctc ctcgatagat gttctcaaca cgctggtcag ggggcaaaag
    23881 ctgccgatat tttctggttc cgggcttcct gccaacgaga tcgcggcgca gatagtcagg
    23941 caggccaggg ttccgggcaa tgaccgttct tttcttgtag tatttgcagc tatcgggata
    24001 actgagagag aggcccgttt tttcagggac tcattcgaga agactggtgc tcttttcaac
    24061 ggtgtatttt tagtaaacct tgccagtgat tctgcgatac agaggttgct gacacccaga
    24121 accgctctta cgatagcaga atattttgcc tttgaaaaag aatatgacgt cctcgttgta
    24181 atgaccgaca tgcttcatta cagcgaagcg ctcagggagg tcagttctgc ccgtgaggag
    24241 gccccgggaa gaaggggatt cccggggtat ctgtattctg atcttgccgg tctttacgag
    24301 cgcgcgggtt gtatcaaggg aaaaaatgga agtataaccc agataccgat cgtaaccatg
    24361 cccggtgacg atatgaccca ccccgtggtc gatctctcag gttatataac cgaggggcag
    24421 atcgttctgg acagggggct tcaggcgaga ggggtatatc cgcctgtaaa tgttctctcc
    24481 agcctgagca ggctcatgaa caagggcata ggcgagggaa aaaccctgat aggtcacagg
    24541 caggtcgcgg accagctttt tgcagcctac gcccggtcga gggagaccgc aagattgaga
    24601 ctgatcgtgg gcgacgaagg gctgacaaat acagaaaccc ggtatttgag tttcggcaat
    24661 gttttcgaga agacttttct tgaccaggga aaccaggcgc gcaaatttgg agagtccctg
    24721 gattcggcgt tgaagtgcct tgctgaactg ccagatgatg aactctacag gattcctgat
    24781 gatatgctgg aagaggtgag ggggtacagg aaagagtgag tttcgagacc ccggtgtcca
    24841 gaagcgaact cgttgaactg aacaggaaaa ttgaaaccat gcggtttgga tgttcgctac
    24901 tggagaggaa gaaggaagcc ctccttcgag ctatagaatc tgacaggtcg accttcaact
    24961 tgatgaggag cgaagtcgag gagatgctgt catttgtctc ctatgcctat gcccttgttc
    25021 agatatttga aggagaatcg gtcatgaccc tcctttcatg gggtatcgaa ccaagagaaa
    25081 taaggatcgt ccatcattct ttgatgggat gcaggtattg tgagtttttc ccccgcgatg
    25141 acagcaaagg gacagctctt gccggactcc tcatggatcc ggcccttgcg tcccttcacg
    25201 tagatgaact gcttgagacg ctttcaaaga tcgaaccggt catctggaaa tatatcaacc
    25261 tgaaggccaa acttgatgca cttgagatgg aattcgagaa gaccagaagg aaagtgaaca
    25321 atctggaaca ggagatgatc ccggccatgg agtcggagcg gtctcgcata aggctttctc
    25381 tggacgagag ggagaggcag gagaccttcg cggtgaaaaa actccttgga agaaaacgga
    25441 gatcgaaggt tccaggaaaa gaagcccctg tcgcaaaggc agttcggcgg accaaagatt
    25501 gaaaatactt gtcctttcag tgggtaaacc caaaaacccc ctttttatca aacaggtcga
    25561 ggattaccgg cagagggttt ctgtgcgtct tccatgtaag tgggatgttg tcagggagag
    25621 ttccggcagg aaaccccaaa atattatttt attggaagag gggaacgccc tgttgaaaaa
    25681 agttggagaa agggattatt ttgttctcct ggatgaatct ggcaggacca tgacaagcgt
    25741 caggttttcg gaatggcttt tccgcaggat ttccgaaaca ccaggaaaga ctgtaatggc
    25801 tgtagggggt gccttcggcg tgtcagcgga agtcagggaa agagctaacg agactatgtc
    25861 actttccccc atgactctca ctcatgaaat gtcccttctt ttcctctttg aacagatcta
    25921 tcgtgcagtt acaattgaaa aaggcgggaa ttaccaccac ggaaatgttg tccgttaaaa
    25981 tgaaaatgac cgaaaggtgc tgaagtgtat gaatatggaa gaaatgctca aacaggctca
    26041 gaagctgcag tcggagatag gaaaggttca ggaagaacta tcccgggaaa aggtggaagg
    26101 agtttccggt ggagggatgg taaaggtaac cgtaaccggt cagggtgaga tcctttcaat
    26161 aaacattgaa cccgaggtca ttgatccctc tgaaaaggag atacttgaag atcttgtcct
    26221 ggcagcgata cacgacggtg tcaggaagag cagagaaatg gcccagggaa ggctcggcag
    26281 gtttggagcg gggctgggca ttccaggtct gggttagggg agaccgtatt ggctgtcccg
    26341 ggtccagttg aaaaactcat gaaactcctg gctaaattcc cgggcgtggg ggacaagacg
    26401 gccaggcgtt ttgcgttgtt tcttcttcag cagccccgct catacacgga cgaacttgtt
    26461 tcgtcaatta cactgatgcg tgacagaacc ttttcatgtt caatatgcgg gaaccttacg
    26521 acagaagacc cgtgcccgat ttgttctgac cctttcaggg accgcaagac cctttgtgtc
    26581 cttgaaaatg ttgaggatat gctctccctt gagcaggccg ggattttctc gggtctttac
    26641 ttcatacttg gaggcaaggt gtctcctttt gatggtgagg acatcgacca ggaaagattc
    26701 tcccatcttt caaggtatgt ggaagaaggc agattcgagg aagtgatcat tgctacgaac
    26761 ccgagcatag agggagactt gactttttat gctgttcttg aatctctcgg gtcagcaagc
    26821 gcaagaataa ccagactggc cttcgggctc ccacttggag ggagtatcgg ttacgctgac
    26881 agggttacac tccacgcgtc tattgagtcc agggttgttg tatcactttc cagagataat
    26941 gaaaaaggaa aggagtgaga aaatggcaga aggaattgtg aaactgatgc ttttactcgc
    27001 aagaattgtt tgtgttcttt tgggtggtgt cgcgggatat cagatcggca ggttttcctt
    27061 gcttcaggga tggtggccct ggaccctgtt tccctacatt ttggctccct actttatagg
    27121 gatattcttt tttgcccttc tgggttatct tgttacacct gtttttgtcc gtacccttgg
    27181 ctttattgga ttatcttttg aaaagaatct ggagtcattg agctggaatg atatttctgt
    27241 tgcccttgcg ggtcttattg caggtctgct ggtggctaac cttgtcgcac tcccctttgc
    27301 ggatcttccc ggaataggta cctatgtcgc cattcttctt aacgttgccc ttggttatct
    27361 cggtgtaacg atctttctca agaggagaga agagatccac gggatgtgga gttccatagg
    27421 gggacttaaa cagaagttca agatcaaggg gaaaaaatcc agagacggca acggagagca
    27481 gactcatgag tacaccattg attcttctga agaacgaagt gccatcaagg ttctggacac
    27541 gagtgtgata atcgatgggc ggatactcga gatcgtatcg acgggttttc tggaaggacc
    27601 ttttattctc ccaagattta ttctgaacga actccagtcc attgctgatt cttctgaccc
    27661 gaccaggaga accaaggggc gaagagggct ggacatcatg aacgagatac agcatctttc
    27721 cgatatctca gttcagataa ttgaaactac tctgaaagat cttgatgccg ggtctgttga
    27781 cgatgggttg gtcgctcttg gtcgcgagct gggggcaaaa gtggttacta cggattacaa
    27841 ccttaacaag ttagcacaga tacagggaat tagtgttctt aacgtcaatg accttgcaaa
    27901 tgcactcaag cccacccttc ttccgggtga agcgataact gtcgatattc tccgggaagg
    27961 aaaggagccg caacagggtg taggttatct tgacgacggt actatgatag ttgtagagga
    28021 tggagaaaac tacatgggta aaagggtcga ggttgttgtt acatcgatgc ttcagacttc
    28081 tgctggaagg atggtattcg gaaggatcag gaaagaggtt cggcattgaa agaatggaca
    28141 tgtattatcg ccgcagctgg tgccggcagc cgaatggggg gtgttcccaa gcagcttgag
    28201 atcctgggag atgtcccgat gtggaagtgg tctttaaaga cggccctttc ccttgccgaa
    28261 gaaggtatca gcgagatcat tgttgtggtt ccccgcgatg ccgtgcctct ctttgacccc
    28321 ggacccgtac cggttggtgt gacctgcaaa gtggtggagg gcggcgaaac acgaagagat
    28381 tctgtactta gtggccttgt gtcagccagt cgtgaaatgg tgcttgttca tgacgcggcc
    28441 agaccttttg cttcagtgga tctctttaaa agagtcatga ccgggacttg taaaaacgga
    28501 gcgacgatac cccttgtccc cgtctctgat gccctcaaat ctgttgaagg ctgccgggta
    28561 aaatggctgc atgaaaggtc cgctgtgaga cgaacccaga ctccacaggc tttcccggcc
    28621 gtggaagcga gggaggtcct ctccctcgcc ggaaggaatg tttctgatga agcggaggcg
    28681 tggctccttg aagggaaaga tctttcctgg gtggagggtg agctcatgaa ttttaaaata
    28741 acctatcccg atgatatgga tatggcacag aggatcgcgg gatcattttt ccttaaaaaa
    28801 accggaattg gttatgacat tcatccactg gctcccggaa ggcccttcat actggggggc
    28861 gttcatgttc catttcccct ggggatttcg ggacattccg acggagatct tctctgtcac
    28921 gctctgtccg atgccattct cggggcttcc ggaatgcccg atatcggaac cctgttcccc
    28981 gccgcggaag aaaaatataa aaatgcaagt agcatctcgc ttcttcacga agtgttgcaa
    29041 agggttcata aagaagggat atctgtagaa tcagcagatg cggtgataac gacccaggtc
    29101 ccgcggcttt cggaatggat cgaggttatt tccaggaacg tgggtaaggt ccttattgaa
    29161 gcgggctgcg atgatttttt actgagagca aaatccggag agtttacggg ccccgttgga
    29221 aatggtgagg caatagcctg ctgggctgtt gcctctatca ggagaaaggt ttgatcgcaa
    29281 agatccgtgt ttaaaattca cttccctgtt catctgcctt tttaatctgc ccgttgcgtt
    29341 gttggccact attgtataga ctcactccca acatcctgtg tcgattccat gatcatggca
    29401 tttccatagg gtggatctaa aacaggggag gtcaattaat ggaaaacatg aaaaacagga
    29461 tcattggaga cggcttcacc ttcgacgaca tgctcctggt tccttcatac agcgaggttc
    29521 acccttcgca ggtagaaccc gggtcttttc tgacacccgg tatcagcctg aatataccct
    29581 tctgcagtgc cgcaatggac accgtaaccg aaagcaggct tgcaatagct cttgcaagag
    29641 agggaggaat aggttttatt caccggaaca tgtccgcaga aaggcagata gccgaggtcg
    29701 acaaggtcaa aaggtctgaa tcgggcgtta ttgtggatcc attcttcctg catcccgagg
    29761 atcttgttaa agatgcagtt gaccttatgg cccattacca tatatccgga gtacccatcg
    29821 tagacgaaaa acggagactg gttggaataa ttaccaacag ggatcttcgg tttgttacaa
    29881 actacgaaca gcctataagc cgtgtcatga cacaggaaca cctcgttacc gctcccgtgg
    29941 gaaccgatct tgagggtgct accagaattc tgatgaaaca caagatcgaa aaactgccca
    30001 ttgtagacga caagggaatt ctgaagggtc tcatcactat caaggatatc cagaaagcaa
    30061 gggatttccc caaggcctca aaggattccc gcggtcggct tcgtgttggg gcggccctcg
    30121 gagtgggcga aggcgccatg gaaagggcag ccggtctctt tgaggctggg atagatgtac
    30181 tggttgtgga tactgcccat gggcattccc ggtcggtcat tgaaatggtg gcttcaataa
    30241 ggaaaaagtt cggtaatatt tctcttgtag ggggcaacat agctaccggg gaggctgcaa
    30301 gagccctgat agacgcgggt gctgacgctg tcaaggttgg tgtcggaccc ggatcgatct
    30361 gtactacaag ggttatagcg ggaatagggg taccccaaat gtctgcagtc atggatgtcg
    30421 cagaagttgc tcactccatg ggtaaaaagg taattgccga tggaggtatc cgttactcgg
    30481 gcgacgtcgt aaaggctttg gctgcgggag ccgactcggt tatgataggc tctctttttg
    30541 ccggaacaga cgaaagtcct ggagaagtgg tgatatacag ggggcgttca ttcaagagtt
    30601 acaggggaat gggctctctc gaagccatga aaggcggaga gagcagggac agatacttcc
    30661 aggaagggca gaccgacgac aaacttgttc cggaaggtat cgaggggctg gtggcctaca
    30721 aggggccaat gagcgcaata gtctatcagc ttgtcggggg attgagatcg ggtatggggt
    30781 atgtgggagc caaagatatt cccgaacttc acgagaactc ccggtttgta aggataacct
    30841 ccgcgggggt caaggagagc catcctcacg atgtagttat cacgaaagaa gcacctaact
    30901 acagaatgga gtaagccttg ttgatattta ctttccgtgc gtggtatact cggatgcgtt
    30961 gaaccgcctt taaatcgatt tgaccggggc gaagagcagg aaaatcctgc tcttcgtttt
    31021 ttatctggat aacggcaggt ggtgtccggt aaatggaaaa ggggagcgaa agtaaacaat
    31081 tgaaggatct tcacgaaaat ttcagggata tcgttgaggg ccaggggtat gaatacgtcg
    31141 gctctgaact ggtatcggag gacgggagaa ctgtcctccg ggttttgatt gacagccttg
    31201 gaggtataaa cgtcaaggac tgcgaaatcg tttcccgaat tatagggaaa tggcttgatg
    31261 attcagatcc gggacttcct gaaaggtata tgcttcaggt cagttctcca ggtgtcgaaa
    31321 gaccactcct gaggcccggg gattttttga gattcaaggg caaaaaagtc aggataaggc
    31381 tcaaagaggc agaaaaggat aaaaagacat tggtcggagt tattgaaaac gttgccggcg
    31441 agatgatcat tattgatgat gaagaaagtg gcaacctgga gatccatttc gaaaatattt
    31501 caaaagcaaa cctggttttc gaaatgccgg gaaagagcaa accggggaaa aaggacagga
    31561 aaagaagggg gcagcaataa tgcagcttgg aagggatttt ctccgagcct tgaaacagat
    31621 aacacaggaa agaaatcttc cactggaagt gatctcatcc gggctcgagg cagcaatgat
    31681 ctccgcgtac aggaaatacc ggggcggaga ccatatcgtt gaagttttcg tcgcactgga
    31741 aagtggagag gtctccattt gtgaggtccg gcaggtcgta gaagaggttt caaataccga
    31801 cacggaactt tctctggata tggcaagaga aatgggtttc gaggatgttg tgccaggaga
    31861 tatcatcagg gttgaggtag aacccgaaaa gtttggccgg attgctgccc agactgccag
    31921 gcaagtaatt attcagcggc ttaaggatgc cgagaggcag gttatcttcg aggagttctc
    31981 tgacaagatg ggagatcttg tcaacggagt aatattcaag tcagagaacg accaggtcct
    32041 ggtgagactc aacgagagaa cagaggcgat ccttcccagg gaggaaagga tcgttggaga
    32101 atcctatcct ctgggcagca ggttcaagtt tttccttttg gaggtcaggc agacgacacg
    32161 gggaccgagg atcgttgtat cgagaagtca ccccgggctc ctcaggaggc tacttgaact
    32221 tgaaataccc gagatacggg agggtacaat agagatcaga gagatagtga gggagtccgg
    32281 ggcaagagcc aaggttgccg tttcaaccct ggatacaaat gttgatcccg ttggagcctg
    32341 tatagggagc gccggttcaa ggatcaagtc catcagcaag gaactgggag gagaaaggat
    32401 cgacgtagtc gtatggagca gcgatcccct gattttcatc aggaacgcac tttctccggc
    32461 gagggttgtc cgggtagagc ctgtgcttga acaggagaaa tccgtacagg tttttgtaag
    32521 gcccgatcac ctttccctgg cgataggaaa agccggccag aacgttcgtc ttgcggcaag
    32581 acttacaaga tggaaaatcg atatcaaggt tctggaacca gagaagcttc cgacccttca
    32641 ggatcttttt gaggaccttg aaattccgga agaggatgaa aaatgcaagg aagagaaaat
    32701 ggaacaggga aacgaaagag acccagaacc tgcgtaggtt gcggagaaga gtttgggaga
    32761 aaaaatcttg tcaggatagt aagaacgccc tcgggggacg tagccgttga tgatggcgga
    32821 aggtctccgg ggaggggcgt atacctttgc agaaaaactg attgtgtcct gaaggcaaaa
    32881 aaaagaaatg cccttgcccg ttcactaagg gtttcggttc cggaagagat atgggatatc
    32941 attcttgaac ttgttgaaag agaggaatct gatcagagtg gttatcaaag ctgagattct
    33001 ggcagagata agaaaccttc tggggttgac aatgaaagca ggggttctgg tcagaggaca
    33061 agactcggta aggacggaac tgaaaaaagg ggcgtcactc cttgttcttc tggccagtga
    33121 tcacagcaag aacgtggctt caatggtcga aggttacagg agaaggggcc aatgcgaggt
    33181 tctggtattt gaaggcctcg aaagagcaag gatggaaaag gaacttgtta ccggatcaac
    33241 ccagatactt ggccttccca gagatcatgg gctgaccata aagatcagga aactttttgt
    33301 gaaaggagct gttgctgatg agcaagataa gaatatatga aatggccagg atgcttgaaa
    33361 agtccaacaa ggaaatgatg gcaatattag gggaacttgg ggtgaaggta aacaatcaca
    33421 tgagttccat tgattcggaa acggcgcaaa tggttgagga tgccatcaaa ggagcaaagg
    33481 cgtccgtgga agaaaaaacg ggttcccccg tttcagaaat taaagttccg gcaggggtgc
    33541 tgatctccga tgtagcgtcc ataatgggaa aaaaaccggc cgaactggtg aaagttcttg
    33601 tttcctccgg atatatgata cccgccgatt ctcctgtaaa cagcgatatt cttgaggtcc
    33661 tttcgataga atttggaatg atcattaccc aggattccgg cgaagaagag agtaaaaccg
    33721 caaaaacagc aaaaaaggca cccctcagag gagaccatct tgttgatcgg gcacccataa
    33781 ttaccgttat gggtcatgtt gaccatggta aaacaacact acttgacttt atcagaaaaa
    33841 cgaggatcac cgaaacagaa gcggggggga taacgcagca cataggggcc tctgccgttg
    33901 tccataacga tcggcgcata gtttttcttg atacgcctgg tcatgaggct ttcactgcaa
    33961 tgagagcaag gggagcccag gcgacagaca ttgtcatact ggttgtagcc gccgacgacg
    34021 gggtcaaacc gcagacactg gaagccctta atcacgcgaa ggcctctgga gtccccatag
    34081 ttgtagccgt gaacaagatc gacaaaccgg gttccagacc cgacagggtc agacagcagc
    34141 tttcagatca cgggcttgcg ccggaagagt atggtggcga tactgttttt gttgatgttt
    34201 cggctgtcac ggggcacggc atagaccaat tgcttgaaat ggttcttata gtcgccgata
    34261 tgctggaaat aaaggccgat ccctctgtct ctccggaggg tgtcgttgtc gaggcaaaac
    34321 tcgacaaggg caagggcccc gtggcgacgg tgctggttca gcaggggact ttgcggaagg
    34381 gcgatattat tcaaacggag gactcctggg gcaagatcag ggcgatgata gacaataccg
    34441 gcaagatggt ggaagaagca gggccaagca ctgctgtgga ggttctcggt tattcgtccg
    34501 ttccccagcc cggtgatatc ttccacgtcg ttgaatcaga aaaggaagcc aggcaggtct
    34561 tcgacgagaa gcaggtttcc agaagggaag cagataccgg gccctcaaga aagatcaccc
    34621 tggaggaaat gtacgacagg atgcagaccg aggaagcacc ggctctctct ctggtgttga
    34681 agtgcgatgt tcagggctcg ctggaggccc tcaaggcttc acttgcaaaa cagggaacga
    34741 gcgaggtcgg tattaatttc attcatgcgg gtgtcgggag aatatctgaa tctgacgtta
    34801 tgctggcctc ggcatctaac gcgataattg tcgggttcaa cgtccggcct gatcccaacg
    34861 caaaaaaggt agcggaagac gagggagtgc agataaggct ttacaggata atctacgatg
    34921 ttatcgagga cgtaagggct gcactcgaag gaatgcttgc tccaaccata agggaaaaag
    34981 cccttggaca ggcagaaatc agggccgttt tcaagatacc cagatcgggc aaggtcgctg
    35041 gttgttatgt tactgaagga atgatgaaaa ggaacgcgaa ggtcaggctc gttcgggacg
    35101 gcgttgtcta ctgggaggga acccttacga cactcaagcg tttcaaggaa gatgccagag
    35161 aagtggcaat gggttatgaa tgcggaatga gtttcgccaa ccagcaggat tttgaggtgg
    35221 acgacatagt tgaggcattt gaacttgtcg aggaaaagag gactcttgat tgatccctgg
    35281 ggatctgttt ctgttttcag gaatactgtt tattcagata aagatcccgg cagcggcgac
    35341 actaaaggac agaagacaga ttaccaggtc catacaggaa agagccaaag ccaggttcaa
    35401 tatctcggta gcagatcttg gtcctgatgg ttcgtgccag gatgcctttc tggtgtttga
    35461 gacagtttcg tcatcccctg atgcaactga ggaaaggatc ctttcagttg agaggatgat
    35521 ccgctctatg gagaataaag gggattttta tattgtgagg ggccaccggg aggtcgagag
    35581 ccgtgcctgg tttcaggatt gacaggataa acagggaact tttaagggag atctccgaat
    35641 tgatacgcac ccggatcaag gatgacactc ttgcagaagc agtcctcttt gatgtggatt
    35701 gcagtcgtga tattgctcat gcaaaagtct ttttcaggac tatagaccca gaccgaaaag
    35761 atgccgttca gaagtccctt gagaaggcct cggggaccct gaggggaatg cttggcagaa
    35821 gaatgaagct tcgcaagatc ccggaactga gatttgttta tgatacgaac gaggatgagg
    35881 cgctgaggat cgaagcaatt cttgacggac tcgacatttt ggtcggtgat gatgagcgta
    35941 atacggagga aagcaaggat tgaagactgt caatgaaatt attcgtattt tacttgaggc
    36001 agatacctgg gtgatcatct cgcacgaaaa accggatgga gatacattgg gttcttgcag
    36061 tgccttttac cagcgaggcc tctcacttgg caaacgatgc atgtggcgag gacccgacag
    36121 gatccccgtg gtgtacgatt tcctgccagg ttcggataaa tacgatgtcc cggttcccgg
    36181 attcctcaga gagatcaggc ccggttccgt cgttattgtt cttgacacag ccaaccagtc
    36241 aagaagtatt gaagggattt ccggtctttc aaaggatatt ccccttgtca atatcgatca
    36301 ccacggcgat aacaaacttt tcgggaccat aaactggatc gatgcaaagg catcctccac
    36361 gggggagatg gttttcgaac tttttgctat tgccagatgg catccgacac ccggggaggc
    36421 tgagtccctt cttgcagcaa tagccactga taccggattc ttccgttttt cctgtacctc
    36481 atcaaagacc ctctccgcgg cgtccagtct tgtcgcttcc ggtgctgata tttcccgcat
    36541 ttatggaaag atccacgaga accgaagtat ccatggactt cgtctctggg gcctggggct
    36601 caagaaagcc cgcacgctac attcgggacg catgggtctg acctggctgg atagaaacga
    36661 ttttaaaacc acaaaagccg atagagaaga gtgcgagaac cttgtcaatt cccttttgac
    36721 ggtttcggga gtggttcttg cggtgttgct gcaggaggag gaggggtatt gcagggcaag
    36781 tatcagaaca agggaacctg ttgacgcgag aaaatttgcg gaaatctggg ggggcggagg
    36841 tcacctcagg gcttcaggtt gcaagataga cggaaccctg gaggaagtat tggaagaagt
    36901 cgggaagaaa gcaggttttc ttgatgaaga cggggtttct tgtagttgac aagaatatcg
    36961 gcgcaaggag cacatcctgc gttgggaccg tcaaaaggat ccttggaaaa gaaaccagga
    37021 ttggtcatgg agggaccctg gattcaacgg cctgcggtgt gctggtattg cttgcaggca
    37081 aggcaacgag ggcttcaaga ttcgtcatgg atcttccgaa actgtatctt gttacggccc
    37141 ggcttggaat aaggacctca acggatgaca tgtccggaga gatcatctcc ataaccgaca
    37201 gggtcgatgt tggggatgga cagatcgaca gggctatttg ctcgatgatg ggactccggg
    37261 aacaggtccc cccatcggtt tccgccgtca gagtcagggg ttcaagggct cactccatag
    37321 ccaggaaagg gctcgtcccg gatatttctc ccagaaccgt aatgatcagt tccataagga
    37381 gaaccggccc tgtctccgaa aagtcggagt tttccatgga gatcaggtgt cacaagggga
    37441 catacattcg aagcattgta agggaccttg gtgaaatttt cggttgtggg gcagctgtgt
    37501 cttttctgca gagagttgaa gttggcccct ttaaacttgg aagtgcggtc ccctacgagg
    37561 tcctatccat atctggacgg gaaatagttg aagagaacct gcttcccctg tcttctatct
    37621 gcgattggtt taccacctac cgcatgaagg gtggtaaccc tgcctctgta ctttcaggct
    37681 gtccggttcc tctggaccac cttgaaagga catcatgggg aaaatatcaa tcccgaggca
    37741 ttgttgtcat cgagggtagg ggttttgtct ctttcggatc aatatctgtt tccggtggag
    37801 taccggcctt ttccccttcc acgaccctgg agagcgggga tatttattga tatgataggt
    37861 gtccttggtt ctttcgacgg gtttcatctt ggccacagga aactttttga aatagcaggg
    37921 agcctggctt cagagctcaa tgattcctgg tgtgtggtta cctttttccc tcatcctcaa
    37981 tccattttga cgaagagccc tttctctgta cttttttcag agccggaaaa ggatattctg
    38041 ggtttttgcc ttggagttcc ggaaatcaac agaatacctt tcacccgtga atgcgcggaa
    38101 cttgaccccc catcctttct ggaactactc gaaagaaatt tttttctcag ggggcttgta
    38161 gttggagagg atttccattt tggccgggaa agatcgggag acgcctcctt gcttgccgaa
    38221 ctggcggatt ccaggggctg gataacatcg atagtcgaat ccttcattat tgacgaccgc
    38281 aaggtttcaa gcagttccat aagaaaaagg atagagatgg gggaaatcca tgaggccgcc
    38341 agggaacttg gttatccttt ttttctgaca ggcacggtaa tacacggaga aggacgtgga
    38401 agaacgatcg gttttccaac tattaacatt cagttgccct tcggcaagat aagacctgca
    38461 agaggagtgt attccgggtg tgcgtccatc aggggaaaat cttttcccgc agcagtaaat
    38521 atcggcagta atcccacatt tcccggagtc agggagatca ggtgcgaggc tcacattccc
    38581 ggttataagg gagaccttta cggagaggat gtatccctgt tccttttcag aaggataaga
    38641 atggagaaga cctttcgttc ccctacggaa cttgtcgaac agatgggatt tgacataagg
    38701 gaatgtttcg ttgactggga gcacatggaa ccccggatga gaatatttat ggaatgttgt
    38761 tcttccggca ttatttcctg aaacggccat tgaagtaccg cctttcccgt ggtaatatat
    38821 ccaacgttct gttgcgggcc tgtagctcag aggatagagc aacggcctcc taagccgtag
    38881 gtcaggcg
//
LOCUS       DAOTIQ010000009        34903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1356_length_34903_cov_8.035267, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000009 DAOTIQ010000000
VERSION     DAOTIQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_34903_cov_8.035267"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1056
                     /locus_tag="P5541_02445"
     CDS             445..1056
                     /locus_tag="P5541_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRV70864.1"
                     /translation="MGAKISEEELKAVIAFHGHWCPGLATGIRISEVALEEFGKSSDE
                     EIVAVAESDNCAVDAVQFLTGCSVGKGNLILKNIGKIAFSFYRRSDGKAIRIVSKSNV
                     ARGTNDEYRGLQDKRNQGTITEGEIARLQELRREHCANILESDLDELFVTGRPIDPVP
                     PFAPMKKSLECSECKEMVMETKARFSDGRTLCPHCFERLVPRP"
     gene            1162..2322
                     /locus_tag="P5541_02450"
     CDS             1162..2322
                     /locus_tag="P5541_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRV70865.1"
                     /translation="MKGAFGARLVVFALLSILAGWGLSIVSESFLASRWNFEDSWVVE
                     MLETGEPVQKDLEGSTDFEGWNTETREIKVLFLSGNFRGTEAVASVEHLKDSRLVMRP
                     GLTYILSYNEFDDGSRIFFVSDVFRAPAFAGLFAGAVVFFCLVTGITGIRAILGLFLS
                     FLVLVKFLLPGLLKGYPPQVLGIAAVAGVALVTVVTVLRRRRCWLPAFSGALGGCVSA
                     ILFGWFSVGFLQLSGLSTESASILSSTIPGLNIRGILLASIMIGASGAVLDVAVSVTS
                     AMAEIKDHDPGITSGGLFRSGINVGREVLGSMVNTLVFAYFGNSLILSLLILEAEPVP
                     IALLNDAVVVEEIVRALAGTAGLLLTVPLTALFGAFASPVKTQPVDNAVSRA"
     regulatory      2426..2546
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2603..3397
                     /locus_tag="P5541_02455"
     CDS             2603..3397
                     /locus_tag="P5541_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70866.1"
                     /translation="MNRSFRERWFMVLMGSFLVIYIIWPLAGMFIRADWRIVASTAKD
                     AQVVGALWRSIWTAAASTAIIALFGTPLAYFLARNNFRGKSVLEAIIDVPIMVPHTVA
                     GIAILMAFSPKAPAGALLVRAGLSPVNSHLGIILACTFVSIPFFVDSARDAFYGVSPR
                     LENVSRTLGATFPHVFFRITLPLAKKGIISGLIMSWARGVSEFGAIVIVAYHPMIAPT
                     LIYDRFATFGLKYSTPIAVQLILVCLAMFVILRLLVAGKRVEEQGS"
     gene            3394..4431
                     /locus_tag="P5541_02460"
     CDS             3394..4431
                     /locus_tag="P5541_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70867.1"
                     /translation="MIRLEDISIDLGDFRLDSFSLSVGEGEFFMLVGPSGAGKTVLLE
                     AIAGLHDPSSGRVLVSGRDVTRLAPERRNVALVYQDYALFPHLTVKENISWGLRFAKD
                     PDLGHVDGLVSLMRLESLLHRFPGTLSGGEQQRVALARALAVKPSVILLDEPVSALDP
                     RFREDLQDYLSRINRDGMTLVMVTHDFNEVLSLGHRVAVILEGTLQQVGEVKSVFREP
                     LNRQVAEFVGMKNLFRAEIGDRKALIEGGATLVLNGQAARGGYLVGIRPEDVIIGRDI
                     PDKGGNCFWGKVVSIVSRGMAFEVILQVGEMKLAGRMLSSSLVREEIHVGGDCRVGFS
                     PESIHVFSKSS"
     gene            4454..5413
                     /gene="wtpA"
                     /locus_tag="P5541_02465"
     CDS             4454..5413
                     /gene="wtpA"
                     /locus_tag="P5541_02465"
                     /inference="COORDINATES: protein motif:HMM:NF003196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HRV70868.1"
                     /translation="MKKFGKCFMFLLVLSLVAVLSATAVFAEGKVVVFHAGSLSAPMA
                     EIESLYEAAHPDVDVQREAGGSAALARKITDLGGACDVFLSADYMVIEKLLRPVTADW
                     NVLFASNAIVLMYGPKSKYREEINGKNWTEVMMRPDVRWGHSEPDADPCGYRSLMVLQ
                     LAENHYADKDLYQRAMKHPERAVRPKAIDLVAMVESGAMDYAFEYKSVAVQHELNYVE
                     LPDEINLKNPDFSETYATASVELAGKEPGQKITVKGEPIVYGLVIPKTAPNNTGALDL
                     VKFILKTGGGLDVFQKMGQDIVGPQAYGETVNVPAGISELLVK"
     gene            5628..5798
                     /locus_tag="P5541_02470"
     CDS             5628..5798
                     /locus_tag="P5541_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70869.1"
                     /translation="MQIIRDHPLHGRDILIDVDSPLPLEEIDKNSGIKYDAEVSRICL
                     KIFREKGYSLAD"
     gene            5833..7155
                     /locus_tag="P5541_02475"
     CDS             5833..7155
                     /locus_tag="P5541_02475"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV70870.1"
                     /translation="MRRSEIQDADILKESFSRDGLKGLFLPGRILFFSAGFVLLWVVS
                     RYNYLLFHVLAEGFSIVVACLIFVLATRTYRFSGNSLLLFLGNAYLAVAIVDLFHTLA
                     FKGMGVFPSNDPNTATQLWIAARYLESFSLLLATWLGNRLPRKIQFWGFLGIASLLVF
                     VVMRTSLFPDCFIAGVGLTNFKIVSEYVISAILVTAMIHLWQIRGNVTPVIFWSLMLS
                     MGTTVLSEMAFTLYTDVYGVTNGLGHILKIISFIFIYNGVVIRGLDEPYGEIFRKLKE
                     TSLRDPLTGIYNRLGFMESAERFFALARREKFGIAMLMMDIDNFKAVNDKFGHAEGDR
                     VLKDFAGILLDCSRKSDIPCRLGGDEFVILMKTNPGGAVIMRNRVEASFLEYQKKESR
                     LGIGLSIGVAFASPGESLFEVDTMMRAADSEMYGYKRSRSQRSAPGGV"
     gene            7163..8047
                     /gene="otsB"
                     /locus_tag="P5541_02480"
     CDS             7163..8047
                     /gene="otsB"
                     /locus_tag="P5541_02480"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRV70871.1"
                     /translation="MNKEKNTRKHLKDIPSVLSRLDEIESAVRGRNLFAALDFDGTLA
                     PLDNDPGAVEMTAEMKKRLKNLALRCPVAIVTGRDLEDIRDRVGLEELFYSGSHGFQI
                     AGPPGSGLSFEAGGEFLPALDKAERLLPGIIEGVPGAFIQRKRFSISIHYRMVRRELV
                     RGLVEKTGRAVKGIGGLVARHDKKSVEVLPRMNWNKGSALLWILDATGMTEANSFAVS
                     VGDDITDEDMHVAVKGRGAGIIVTGTEPPLSTFADYRLGSVAEAGRFIEILERIAGNV
                     TRGQESPAGGTQASPPIT"
     gene            complement(8044..8631)
                     /locus_tag="P5541_02485"
     CDS             complement(8044..8631)
                     /locus_tag="P5541_02485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV70872.1"
                     /translation="MTDHLSGEKFEQRDFEKPGLLFRFCLAAIHPFFTGPIIYAPFVE
                     KLGLEGNERVLEFGCGNGVLMTYLAKSLGKGGSATGVDTSSYMTDRARKRLAPFSNAE
                     ILRGDIRTLELRPGSFDLVIFIHVLHDIAPEKRQGIMDALSRLLVPGGRLCLMEPVSP
                     FSRHAGGGDPVIGRECRSQCHRDSTYGPEGEGLLS"
     gene            complement(8698..9126)
                     /locus_tag="P5541_02490"
     CDS             complement(8698..9126)
                     /locus_tag="P5541_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRV70873.1"
                     /translation="MAKIVAVCTSGKRRDPKEPLQEAVFSEGSGIEGDSHFGMVERQV
                     SLLRAEDIRKAEKEAGFEFPPGSLAENLVIEGLPEELVPGQTLSIGNRVLLSITEKGK
                     RPGEPHSYNYRGWCLLPTEGYFLSVEKGGTVRPGDEVILK"
     gene            9215..9517
                     /locus_tag="P5541_02495"
     CDS             9215..9517
                     /locus_tag="P5541_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRV70874.1"
                     /translation="MRKKAILALAVLMTLIFVVPDSISADTSGVARFSYDFFGLRFDV
                     EFVDDAAVLYLPSGPLKLPQVISGSGARYSDGETTFWIKGDDAYMEINGSVAEEAD"
     gene            9536..9889
                     /locus_tag="P5541_02500"
     CDS             9536..9889
                     /locus_tag="P5541_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HRV70875.1"
                     /translation="MDLSFFSVLADIVSVAGICVILWGSGIALWRFFRVEKNMFSGKN
                     LQMKRESLRHSFGSYLLLGLEFLIAADIINTVMKPGLHEVAILGAIVVIRTVIGYTLD
                     HELSSEADSRHKQGE"
     gene            complement(9893..10849)
                     /locus_tag="P5541_02505"
     CDS             complement(9893..10849)
                     /locus_tag="P5541_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HRV70876.1"
                     /translation="MIRAKKGFTLVELLVVMVVVGILAGSLMLLFGSASNRSTATRMV
                     SDLMTLKRASVIFFYDTGEWPDEDDHEKLNSYMIRDVLAEGWGDGTRTYAIQRPDDSG
                     AVYAAARVGDEYGGSPAIRKLLEKEANKFELVNTNGTPFRSTDDTVMVLIRDTASGSD
                     PDDPGTDPGDDPGDDPGGGEEPETPDYSEWDNGAVYLGGQMVIYDGRVFQARYWTQGQ
                     QPGLLESPWQEITDEWTFFNIYDGGDIILVDGKQYQAWYWTQNETPGIVGNPWQELTD
                     QWVPSNRYYAGDTIFHNGLQYRAKWYSVNSTPGSSSSWELVP"
     gene            11052..11555
                     /locus_tag="P5541_02510"
     CDS             11052..11555
                     /locus_tag="P5541_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV70877.1"
                     /translation="MIRLETDKGDILVELDMEKAPKTSENFINYVRKGHYDGTIFHRV
                     IDGFMIQGGGFDEKMNQKPTDAPIENEATNGLKNEAYTIAMARTQDPHSATAQFFINV
                     KDNIFLNHTAPNTRGWGYAVFGKVTEGHDVVDAIKRVRTGNCGGYQDVPEEPVKIIRA
                     SIEGEDA"
     gene            11552..12094
                     /locus_tag="P5541_02515"
     CDS             11552..12094
                     /locus_tag="P5541_02515"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRV70878.1"
                     /translation="MNREVRAVVDPLLEAAGFTDCLWVSPSSMIVSQWVRMKCMFGCP
                     HYGKNASCPPNVPTVSECRDLLSEYSMAVLVHLVCDIPSQGKKEWCRTMQKELLEAEK
                     QIFLAGFERTFLLSFDNCNLCDECVPSPSACLQPCLRRPTMEAFAIDVYSSVKGHGYE
                     VSVRRSPEDPEDRFALILVK"
     gene            12104..14281
                     /locus_tag="P5541_02520"
     CDS             12104..14281
                     /locus_tag="P5541_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRV70879.1"
                     /translation="MDLRYASAIASELGIKEEQARQTGLLIDEGCTVPFIARYRKEAT
                     GSLDEVAVMAIRDRFSQMEELDKRRIAILKSLGERDLLTEELEKAVNSAPNMTALEDI
                     YLPFRPRRRTRATVAMEKGLEELAVLLMKAASGNLDPFDPLEEAGRFVDLDKGVSDTA
                     EALSGARDIVAERVSEDRRSRVVLRSIFARNGRLSSRVIRGKEDKGAKYRDYFDLEEP
                     ARSAPSHRILAMFRGEKEGFLGVSARPPAELAIRELEKIFIKAANGAAEEVRAAITDG
                     YERLAAPSMETELKNALKLKADREAIRVFSGNAREVLMSPPLGSSPVMAVDPGIRTGC
                     KVVVIDAQGRLLMDDVIYPFSGKKPFEEACRKVVDIMERFPVDAVAVGNGTAGRETEA
                     FLKGLHLPGDPPVILVNESGASIYSASKAARDEFPDSDVTVRGAVSIGRRLQDPLSEL
                     VKIDPKSIGVGQYQHDVDQKMLVRSLEDVVVSCVNSVGVDLNTASPQLLSFVSGLGPG
                     LAGNIISWRNTNGSFSSRSQLKDVPRLGPRAYEQAAGFLRVRGGEHPLDASAVHPDHY
                     GIVGKMAADLGCSVDDLLADETLRDRIEPDRYICPEAGLPTLRDIMAELSRPGRDPRK
                     SFETVNFSPDVKKIEDLVPGMELDGVVTNVTAFGAFVDIGVHQDGLVHVSRMADRFVK
                     NPSDVVKPGQGVKVSVIEVDIPRKRISLSMRKDKKDSERPGSD"
     gene            14344..15627
                     /locus_tag="P5541_02525"
     CDS             14344..15627
                     /locus_tag="P5541_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRV70880.1"
                     /translation="MRPRVFLVLIVSVALLFIKGQARADVFQVQVLSLEECVEIALSG
                     HPDLAAARSDLEASRARVGQARTQDNPSLAYSTSYTERGSSLQGSSGGLWSSSLTLSQ
                     LVTDWGKTHSSVKRSLSEQEISSLSLKQALLDVEYDVRRAYFLLLKAEKNLEVAKETM
                     SINELQLEKAEAFFEAGRVSRYDVTAAQVSRSNANMALIQARTSRDNAMSDLRKAMGF
                     TGAEFGVSDPEGLSEMTENKNLPLLEQALQAAYEKRPDLLSWKNRVESARLSVTLAKL
                     DNAPKLSLSGSFGWGSSSFFGDEDWRGALTLSFSLYDGGLQKEKTREALSSMQAVEAR
                     CESFRQTVATEIESALSRARDSESEIEAAGEGLRLAGENLEIATGRYQVGVGSPIEVS
                     DAARNYIDAKVAWHSAIYDRMIALAALEKAIGGSR"
     gene            15627..16814
                     /locus_tag="P5541_02530"
     CDS             15627..16814
                     /locus_tag="P5541_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV70881.1"
                     /translation="MKKKSVIIAFLVLAAAGGALYLRFGGKDTPVVQTTPVIRGDIVR
                     SVSATGRIQAVNTVNIGAQVSGTIRVIHVDYNSVVKKGQLLAEIDPSLLEASVRQAQA
                     SVRSAKASLAEARAVLDDAAKNLRRSEDLFSRGYISKTDFESAQSTHTAALARVETTG
                     ASLAQAEADLMYKSINLGHTRIVSPIEGVVIDRAVDQGQTVNASQSAPTLFSIAEDLS
                     RMQVEASIDEADIGLISRGQTAKFTVDAFPDVDFEGTVREIRLSPSTSENVVTYTVVM
                     GVENHELKLMPGMTANVTVIVESRNDVLKVASSSLRFRPPNAEQQPRKQGGQNGQGRG
                     AAIWSFEEGKLERHSVKPGISDGMYTEISGDIEEGMEAVTAIDYGSSSSGGGAAFGIA
                     PRR"
     gene            16792..17484
                     /locus_tag="P5541_02535"
     CDS             16792..17484
                     /locus_tag="P5541_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV70882.1"
                     /translation="MELLRADNLSKTYLMGDVNVQALSGVSFSVTRGEFISIMGASGS
                     GKSTLMNILGCLDTPTDGKYILEGKDVSGLSRDELADIRKHRIGFVFQGFNLLPRMSA
                     LENVKMPLLYNEIPAHERDPRAMEALEMVGLGDRANHQPQQLSGGQQQRVAIARALVN
                     GSSIILADEPTGNLDSEASRELMDLFSRLNSKLGRTIVQVTHEQDMAAYTGRIISFRD
                     GRIVSDSGVERK"
     gene            17481..18707
                     /locus_tag="P5541_02540"
     CDS             17481..18707
                     /locus_tag="P5541_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70883.1"
                     /translation="MIALSETTKIAFRALLVNKTRSALTMLGIVIGVMAVIMMFAIGA
                     GANRQIAERFSSMGSNMIIVRPGASQGPGGVSGISSRNLTIEDAYAIGSECPAVAYAA
                     PVSSGNAQVVYGNQNWSAPVTGTTPDYLFVRDWGLDSGHSFTEQDVRSAAKLAIIGKT
                     VAENLFGNEDPVGKTIRVLRVPVVVVGVLGEMGESAFGRDEDNIILVPITTAQRRLFS
                     TSRIGSVSSIMVKATGSGMLEAAQKQIEMLLEIRHRIRPGGEQDFSVRNMSQMIENAK
                     SATRIMTLLLTAVASVSLLVGGIGIMNIMLVSVTERTREIGIRMALGARTVDIRLQFL
                     VEAAALSMLGGFVGILLGITGSQVISRGFGWAVEISPFSVVLAFGFSALVGIFFGYFP
                     AVKASMLNPIEALRYE"
     gene            complement(18781..18927)
                     /locus_tag="P5541_02545"
     CDS             complement(18781..18927)
                     /locus_tag="P5541_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70884.1"
                     /translation="MKDNPVRLVFMGGVPHWRLPEGNFLKAETYDGKIVETRIRLKSA
                     HNTW"
     gene            19143..19934
                     /locus_tag="P5541_02550"
     CDS             19143..19934
                     /locus_tag="P5541_02550"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRV70885.1"
                     /translation="MLKEPVIIYVFSGSGNTWTVARKMADELLECGVATELRPLRREG
                     PGDISRAGTLGIAFPVAMFTAYPFVWEFVGNLPDGKGMDVFMVDTLAGFSGGVVGPMR
                     DAVSAKGFRPIGAKEVRMPSNYARTDPGGEKDNENRTRGLNKAASYARDLFDGKTSWR
                     RVPIVPDLVRAVGKNPDGSAWRSIRRKFPLEVNSEKCTRCGLCLKLCPVGNITMEEIP
                     RFGKNCFFCQRCISFCPEEAISVSGKNYVPYRGMDKKTLLEFLGI"
     gene            20120..20404
                     /locus_tag="P5541_02555"
     CDS             20120..20404
                     /locus_tag="P5541_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRV70886.1"
                     /translation="MSEKWKDELTEQLAKALLTLKTSEEVYDFLEDVATIGEIRALAQ
                     RLEVAKRLNEGETYPVIAQQTGASTATISRVKKCLDYGADGYKNVLDKMK"
     gene            20484..21371
                     /locus_tag="P5541_02560"
     CDS             20484..21371
                     /locus_tag="P5541_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70887.1"
                     /translation="MASLFRFFAFALLVILSLVAWAFFDYSRNRRKAASYFRERLGMA
                     KDLFTMTRFVNMARVVIPETERFIAVFFRKGEHLEIRDFAPDRVIDIPPDSVLLSDSR
                     RNLTWIFVERSKTIFSLKVTDFLPGTLCHVRKGTGRVRFGDEDIPASNRDWFLMDRER
                     GVSFSLPNGKEKDKEEGFFFYDGFAPTEGFLLDEDGGVLLVDEKRGTFGFREVAGEAL
                     TLYRPDDIVSAAPSVEGPDFVDITVRDGKRPRFTFEFDDEGEARYWMEWFVRVRDEMS
                     GKGTRTGTRFVRISPVKIS"
     gene            complement(21368..22186)
                     /locus_tag="P5541_02565"
     CDS             complement(21368..22186)
                     /locus_tag="P5541_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRV70888.1"
                     /translation="MIPETDKQQNLAVLIDADNTSPSIIDNLFAEIAKYGISRVKRIY
                     GDWTSPYLKGWKEVLLSYAIHPIQQFHYTVGKNATDSAMIIDAMDLLYSSRFDGFCLV
                     SSDSDFTRLATRIREAGITVFGFGKKGTPNAFVSACDKFIYTEILGKDEEEEEQEHTI
                     GEGSTNKKPQSPRLLRGDSKLTNLLRNSVDAVADEEGWAALGNVGQHISNQSPSFDSR
                     NYGFRKLSSLVKATGLFEVEARAVDNESGNKAIFIRHKERKGNTPKKGSTRQAP"
     gene            complement(22223..22975)
                     /locus_tag="P5541_02570"
     CDS             complement(22223..22975)
                     /locus_tag="P5541_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70889.1"
                     /translation="MTSHPRRESIILPVLLATGYTAMLLAEPFWEKILRMLFPSRAQV
                     VYERSSMITLAWEHLVLVLVSSLLATILGVIIGILITRKSGRDFIPVVDDITSIGQTF
                     PPVAVLALAVPSLGFGFQPALFALFIYGLMPVLRNTVTGLESVSPDILDAAAGMGMKP
                     VQSLLKVELPLAVPVIMGGIRTSAVISVGTATIGATIGAGGLGAPIISGLISQNPAIV
                     LQGAISAAFFAVVLDSILKKIESMLSGPRDAL"
     gene            complement(22972..24162)
                     /locus_tag="P5541_02575"
     CDS             complement(22972..24162)
                     /locus_tag="P5541_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV70890.1"
                     /translation="MGLFSRGTEISPRGFDRVITAGSLLVMAPLFLNPFVVVRETRIS
                     PPHGVGAVEAMGFFPVAVILSLAVAAFFLAGKGPARRRNIASATLSRILFLAILWQAG
                     ASATRISVPAMPFARVSIGSGAWLALLGSFMIMSPCRRAAREEKLFVFFCWGFYAALA
                     FLFFSGGMDNISFLKEYHNRSGRFIQELARHIALSSSAVSAGLLTGFPLGLVALRNKR
                     LGSAIISLANTLQTIPSLALFGLLIAPLAWVARSFPSLASIGVGGIGWAPAFLALTIY
                     SLLPIIKNTVSGFSIIDPSIVDAGSGMGMSKKQLFLRVELPLAMPVVLAGVRISGVQA
                     IGNTAVAALIGAGGMGQFIFQGLGEAAPDLILLGAIPTVMLAITADWGFRKLAEILGP
                     GETR"
     gene            complement(24166..25122)
                     /locus_tag="P5541_02580"
     CDS             complement(24166..25122)
                     /locus_tag="P5541_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV70891.1"
                     /translation="MKDKGRSSGRVLAIAALLAVLLAVALPLSAPQASSAQEKIIVGS
                     KIDTEGGLLGQMIVLLLRENGFSVNDRTSLGSTNVVRQAIIAGEIDIYPEYTGNGYYF
                     FDDSDTEIWKNPEKAFERVSTLDLEKNGIVWLQPAPANNTWAIAVRKDLSEKEGLSSL
                     EDLATFINAGGKIRLACSEEFATRPDALPAFESAYGFKLGREDLLILSGGNTAQTEQA
                     ASLGRDGVNAAMAYGTDGGIAVMDLVVLEDSMGVQPVYEPAPIIRKEMIEKNPVIADI
                     LNPVFSTLDLVTLQKLNGRIAVEGQDPARVAEDHLRSLGLLK"
     gene            25334..26620
                     /locus_tag="P5541_02585"
     CDS             25334..26620
                     /locus_tag="P5541_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HRV70892.1"
                     /translation="MTFSALASTAVFAATIIAIAMGKMQRSVASLLGASLLMAFHVIP
                     AEKAVTYIDFNTIGLLMGMMIIVGVLARTGVFQYVAVKTVKFTGGNVRLTFVAVIAVT
                     AFLSAFLDNVTTVLLISPVVISLVDLIDVNPVPFLLGEAFASNIGGAATLVGDPPNII
                     IGSFAGFSFMDFILNLTPASVLVLVIVCGWLLFHFRKEFTVSETSAERIAQVDESRLI
                     RDPLLMRRASGAMLLVLAGFAVHHLIHLPASVVALLGASLLLAVVPVSGDEVVHRDIE
                     WPTIIFFASLFIIVGALRETGVITAVSNIMAAMLQGHPFLALLAVLWFSGLSCCFINN
                     VAFSATFVFVARDLALSLGMAASPLYWALALGACLGGNGSFLGSAANVIVADMAEKGG
                     SRISFQTFMGAGLKVVLISLSVASVYIMIRWVLLGG"
     gene            26690..27145
                     /locus_tag="P5541_02590"
     CDS             26690..27145
                     /locus_tag="P5541_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV70893.1"
                     /translation="MLVEEIMDRDLTAVSPDTTVAEVVEILSAQRISGVPVVDDEGRV
                     IGFLSEKDIVKAALPGYFDLLQDSSFLPDYGQFTRRLRMVSLDSVDKYMRKDVIVFDE
                     KDTDLHVAMVLITRNLKRAPVVRDGLFAGVISRADLLDHILRSRKEPSD"
     gene            27142..27411
                     /locus_tag="P5541_02595"
     CDS             27142..27411
                     /locus_tag="P5541_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70894.1"
                     /translation="MTRAGRIELRFHGPWLRVAGKPTMTYEIDGPVLLEELLSALEKI
                     FGSGFSSHGEGLVCLLEDSGPRALKMKTPVLPGSTLVFVGTVESG"
     gene            27435..29276
                     /locus_tag="P5541_02600"
     CDS             27435..29276
                     /locus_tag="P5541_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRV70895.1"
                     /translation="MNGYHGRIAVVDLAAKRSRVMRFDEDFARKYIGGSGMGTYLLLT
                     FCTPAVKPLSPANPLIFMTGPFMGTGIPTSGRHQVLTKSPLTGIFLESDAGGSFGFHL
                     KRSGFDGLVIVGKASDITVLILRDGRVEFEDGNDFRGLDTMETDAKLRGIYGKNMGIS
                     CIGPAGEKQVPIAGIFHDGTDARTSGRGGAGAVMGSKNLKAIVAGGSEMAPLKNRKKL
                     GELISIKNSTLKEKGSGLTLYGTSGGMGLCEELGDLPIRNWKQGSWKEEVKAITGQKM
                     AETILSGNFGCVACPIRCGRVVKIDGREVGGPEYETMAMLGSNCLVDDLDAIARANET
                     CNRLGIDTISAGSAVAFAMECFENRIISEEQAGMPLEWGSGDALNFLVGKMGRKEGIG
                     ALLGKGTRAAARELGGGSDRFAIHCKGLEFPAHDPRCFKSLAAGYATSTRGACHLNGY
                     SYAWERSASFPEFGYNEKQERGTDEGKGIMNARFQNLSSLADSLKICKFPLLLGVSLS
                     DISEWMQCVTGWDMDQKELERAGERSYNMKKLFNVGHGLSRKDDILPERIIKEPRGSG
                     GSAEALPDLELQLDEYYSYRKWDENGLPTVEKIEELGLREFIHWLVG"
     gene            29407..29667
                     /locus_tag="P5541_02605"
     CDS             29407..29667
                     /locus_tag="P5541_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRV70896.1"
                     /translation="MEVLKVSANSQPKSVAGAIAAVVRESGAVEVQAVGAGAVNQTVK
                     SIAIARGYVSPNGIDLVCIPSFAKIDIDGEERTAIKFQLESR"
     gene            29973..>30166
                     /locus_tag="P5541_02610"
     CDS             29973..>30166
                     /locus_tag="P5541_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRV70897.1"
                     /translation="MSSISAPRGVRDILPDESWKWEHVLDVTARTAKDFGYREIHLPV
                     FEHTELFSRGVGETTDVVEK"
     assembly_gap    30167..30266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30267..30572
                     /locus_tag="P5541_02615"
     CDS             <30267..30572
                     /locus_tag="P5541_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV70898.1"
                     /translation="RQPLLDAFVVCSEEGIRDEAVKLLYELRSAGIAADIDYSGRSFK
                     AQFKFAGGSGASFACVMGPEEVSSGSVTVKDLESGEQRRVARGDVASAIMASGHKNG"
     gene            30598..32421
                     /gene="aspS"
                     /locus_tag="P5541_02620"
     CDS             30598..32421
                     /gene="aspS"
                     /locus_tag="P5541_02620"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRV70899.1"
                     /translation="MKQRFFDSTWKRSCYCGDIPLPCEEGPEVVINGWLRRRRDLGGI
                     IFLEIWDHTGVVQVVFNPELGPLYERAKSLRNEFVLAIRGRVRKRPEGTENPSMPTGD
                     REVVARDFLLLSPSRQLPFEVSEADRVDENLRLRTRYLDIRRPVMQKNLRTRSKVASF
                     TRNFLSGEGFVEVETPMLTKSTPEGARDYLVPARVMPGRFFALPQSPQIFKQILMVGG
                     IDRYYQIVKCFRDEDLRADRQPEFTQIDLEMSFITEEEIISLMEKFMKGLFSDVSGID
                     LETPFPRITWRDAISRFGSDKPDLRIDSEILDLDNVFAASSFEPFIKTLEQGGSVRGL
                     HIPGGASLSRKEISDLETQAKSFGASGLAPFSMKDGVLKGPLVKFLSEDLQKTLVENV
                     GLNEGDALVVAGGAWLETCEALGHLRLELADRFYRSDESKWAFLWVTEFPLLEWDEEG
                     SRWSAVHHPFTSPNLEDLEKLSDSPGNVRSRAYDLVLNGTEVGGGSIRIHDPEVQQKM
                     FEVMAFTPEKARERFGFLLDALASGTPPHGGIAIGFDRLVMLMCGVHSIRDVIAFPKT
                     QKAQCLMSGAPSDVDPVQLSDLHIALDIRGEEEECGSGGAE"
     gene            32397..32681
                     /locus_tag="P5541_02625"
     CDS             32397..32681
                     /locus_tag="P5541_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70900.1"
                     /translation="MRVRGRGIDIDGERMPWYYDLEESAGELKGQLKVEGWDPSAAMN
                     SWYERSKGKKIGIMIPGLGRAILSPTGIFIHESGHHNETEVIVRGSLKTA"
     gene            32745..33425
                     /locus_tag="P5541_02630"
     CDS             32745..33425
                     /locus_tag="P5541_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRV70901.1"
                     /translation="MARIRFLGHSAFHIEGEGLNGLVDPWLSGNGMSPFSPDDFPEVN
                     FIFVTHGHGDHLGDAPAIAEKTGAMVVGTVELCAALPEGVKTHPMQIGGAFRFPFGKV
                     RMTPAWHGSGIMADGVMVYGGVAGGFLIEVDGKRIYHAGDTGLTMEMTLLAADSVDVA
                     LLPIGGNYTMDIEDAVRATEMIRPRIVIPMHFDSFPVIKASPERFSSLASGHAEVRVL
                     GAGDSLDI"
     gene            complement(33428..34297)
                     /locus_tag="P5541_02635"
     CDS             complement(33428..34297)
                     /locus_tag="P5541_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRV70902.1"
                     /translation="METAQLQELLREKMQGMPNKARRVVEYLLKNMREAAFRSIGDVA
                     DDLGVSKAQLVRVSRMLGFEGYSQLKDALQGAMLGQVNPAAMLARVLNNGQDLPSTIH
                     RLEHANLDDTWSQIRPEQVSSFCEMIQGADSVYCVGWGISSMVSESLFIRLRTMGIRA
                     ILMTRSGMTLQEQARSIGEKDLVVVCELPSFVIEVTEAVEIAKKQGARIATITDSPAA
                     PICRHSDLPFFVSAASPMFGSSIIGPLFLVHILTSVLAVNMGEATRKAMDEQAEFLHD
                     ERIFHPVFGLKYS"
BASE COUNT         8036 a         8452 c        10021 g         8294 t
ORIGIN      
        1 caggaagcag gaaacaggaa acaggaaaca ggaagcagga aacaggaaac aggaagcagg
       61 aagggcaaga gccttaaatt caactgcgta aacggtgaac ggtgaacggt gaacggtaaa
      121 cggtgtgggg cagaagaccg aggagactgg aagcaggaaa caggaaacag gaagcaggaa
      181 gggcaagagc cttaaattca actgcgtaaa cggtgaacgg tgaacggtga acggtaaacg
      241 gtgtggggca gaagaccgag gagactggaa gcaggcctct ggagacaggg aagtcaagat
      301 cttgtcattc tgaggaatga gatgacgaag aatctggttc tgacccggat tcttcatccc
      361 gctggtcctc agaatgacaa acaaattcaa ctgcgtaaac ggtcaacggt gaacgggagg
      421 aaagtatcca agggaggtaa tattgtggga gcaaagatat ctgaagagga acttaaggcc
      481 gtcatcgctt tccacggcca ctggtgtccg gggcttgcca cggggataag gatctccgag
      541 gtcgcactgg aagaattcgg aaaatcctcc gatgaggaga tagtcgccgt agcagaatcg
      601 gacaactgtg ccgtcgacgc ggtccagttt cttacggggt gttccgtagg caagggaaat
      661 ctgatcctga agaatatcgg caagatagcc ttcagcttct atcggcgaag cgacggcaag
      721 gcaataagga tcgtaagtaa atccaacgtt gcccggggaa ccaatgatga atacaggggt
      781 ctccaggaca agagaaatca ggggacaata acagaaggtg agatagcaag gctccaggaa
      841 ctaaggcggg agcactgtgc caatattctg gaatccgatc ttgacgaatt gttcgtgaca
      901 ggaaggccca ttgatcccgt gccgcccttt gcccccatga aaaagagcct tgaatgttcc
      961 gagtgtaaag agatggtgat ggaaaccaaa gcccggttct ccgacggacg caccctttgt
     1021 ccccactgtt tcgaaagact tgttccaagg ccctgaaaat ttcctggcga tctttgtcaa
     1081 agggctgttg attgcccctg accccgtgct ctgttagagt cgtgtggttc ggatcgttat
     1141 tttgtacggg gagtggtgaa cttgaagggg gctttcgggg ccagacttgt cgtttttgcc
     1201 ttgctgtcga tactggccgg ttggggactg tccattgttt cggagagttt cctggcatca
     1261 aggtggaatt tcgaggacag ttgggtagtg gagatgctgg agaccggaga acctgttcag
     1321 aaagacctgg aggggtcgac tgactttgaa gggtggaata ccgaaaccag agagataaag
     1381 gtccttttcc ttagcggtaa tttcaggggt accgaggccg tggccagcgt ggagcatctt
     1441 aaagacagca ggcttgtgat gcgcccaggt ctgacatata tcctctccta caacgagttt
     1501 gatgacggca gcaggatatt ctttgtcagc gatgttttcc gcgcccccgc ctttgcgggt
     1561 ctttttgccg gtgcggtcgt ctttttctgt cttgtgacgg gaattaccgg aatccgcgcg
     1621 atcctcgggc ttttcctttc gtttctggtc cttgtcaaat ttctccttcc aggtttgctc
     1681 aagggctatc ccccgcaggt actcggcatc gccgctgtgg cgggagtggc tcttgtgact
     1741 gtcgtgacgg ttctcagaag gaggcgttgc tggcttcccg cctttagcgg agccctgggt
     1801 ggctgtgtat ctgctattct tttcggctgg ttttcagtgg gttttcttca actctcggga
     1861 ttgtcgaccg agagcgcgtc aatcctctca agcactatac cggggctgaa tatccgcggt
     1921 atactccttg cctcgataat gattggcgcg tcgggggctg ttctcgacgt ggctgtttcg
     1981 gtgacctctg ccatggccga gataaaggat cacgatcccg ggataacctc gggcggtctt
     2041 ttcagatcgg ggataaatgt cgggcgggag gtgctgggca gcatggtcaa tacccttgtt
     2101 tttgcctatt tcggcaattc cctgatcctc tcgcttctta tcctggaggc cgagcctgtc
     2161 cccattgccc tccttaacga tgccgtggta gttgaagaaa ttgtaagagc ccttgcggga
     2221 actgcaggac ttttactgac ggtacctctc acggcattgt tcggggcttt cgcttctccc
     2281 gtaaagactc agcctgtgga taacgcggta agtcgagcat gatcccggcc tgacgatatt
     2341 ctcccgtggt cctgacattc gtgaccaggg cgtctgggtc tgttgtaacc agttactcta
     2401 tgtgatagca tttccgtgcc agaaattata aagcatcctc cgagccgaat cccctcaggg
     2461 agttttctca ccggggaccg gccgacgggt tctcctggaa acgggagggc ctcccgtgtt
     2521 tggaaaggag gagcggtcgg gtgttgcaat gggccgtctc ctgtatccgg agacggccca
     2581 ttttttgtct ggaggcttgt aaatgaatcg gtctttcagg gagcgctggt tcatggttct
     2641 gatgggttct tttcttgtca tatacataat atggcccctt gccggcatgt tcatcagggc
     2701 cgactggaga attgtggctt ctaccgccaa ggacgcccag gtggtgggag ccctttggcg
     2761 cagcatttgg accgctgccg cttcgaccgc gatcattgcc ctcttcggga cccctcttgc
     2821 gtacttcctg gctcgcaata acttccgggg caagtcggtt ctcgaggcca taatcgatgt
     2881 tccgataatg gtccctcaca ccgttgcggg cattgccata ctgatggcat tctctcccaa
     2941 ggctcctgcc ggggctctgc tggtaagggc gggcttatcc ccggtcaaca gtcatctggg
     3001 gatcatcctg gcctgtacgt tcgtttccat acctttcttt gttgattcgg cccgggatgc
     3061 attctacgga gtctctccca ggcttgaaaa tgtttcccgt accctggggg caaccttccc
     3121 tcatgtcttt ttccgcataa cactgcccct tgcgaaaaag gggatcatct ctggtctcat
     3181 catgagctgg gcaaggggcg tcagcgagtt cggcgccatt gttattgtcg cctatcaccc
     3241 gatgatcgct cccaccctca tctacgaccg gttcgccaca ttcggtctaa aatattcgac
     3301 cccgatagca gtgcagctta tccttgtgtg ccttgcgatg ttcgtcattc ttcgtctcct
     3361 ggtagccggg aagagagtgg aggaacaggg ctcttgataa ggctggaaga tatttccatc
     3421 gacctcggag atttccgcct tgattccttt tctctttcag tcggtgaagg agagtttttc
     3481 atgcttgtcg ggccgagcgg tgccggcaaa accgttctcc tggaagcgat agcggggctg
     3541 catgaccctt cttccgggcg ggtcctggtt agcggaaggg acgtgaccag gcttgctcca
     3601 gagagacgga atgtagccct tgtttaccag gattacgctc tttttcctca tctgacagtt
     3661 aaagagaaca tctcctgggg gctcaggttt gccaaagatc cggatctcgg gcacgtcgac
     3721 gggctggtaa gcctcatgag gcttgaatcc cttctgcaca ggtttccggg gacgctgtcc
     3781 ggtggagagc aacaaagggt agcacttgcg agagcccttg cggtgaagcc ttccgttatt
     3841 cttctcgatg aacctgtctc ggcactggac cctcgtttcc gggaggacct gcaggattat
     3901 ctctcaagga tcaacaggga tggaatgact ttggtaatgg taacccacga tttcaacgaa
     3961 gtgctttcgc tcggtcatcg ggtcgcggtc attctcgaag ggacactcca gcaggtggga
     4021 gaggtgaaga gtgttttcag ggagccgctc aacaggcagg tggcggagtt cgtggggatg
     4081 aaaaaccttt tccgggctga gatcggggac agaaaagcac ttattgaagg aggtgcaacc
     4141 cttgtgctga atggccaggc agccagaggc ggatacctgg tcgggatcag gccggaggac
     4201 gtaattattg gccgcgatat ccccgataag ggggggaatt gcttttgggg caaggtcgtt
     4261 tcgatagttt caaggggaat ggcttttgaa gtgatccttc aggtcgggga gatgaaactt
     4321 gcggggagga tgctttcttc ttcgctggtc agggaggaga tacatgttgg aggggattgc
     4381 agggtgggat tcagccctga atcaatacat gttttcagta aaagcagtta aaaaatctgg
     4441 aggtgtcaag atcatgaaga agtttggcaa gtgtttcatg ttcctgctgg ttctgtcgct
     4501 agttgcggtc ctttccgcaa cggctgtttt tgcggagggt aaggtcgtcg tctttcacgc
     4561 gggaagcctt tctgccccca tggcggagat agagtctctc tacgaggcgg ctcacccgga
     4621 tgtggatgtc cagagagaag ccggcggaag cgcggctctg gccaggaaga tcacagatct
     4681 tggaggggca tgcgacgtgt tcctttcggc agactacatg gtaatcgaaa aactgctccg
     4741 cccggtaacg gccgactgga acgttctttt tgccagcaac gccatagttc tcatgtatgg
     4801 accaaagtca aagtatcgtg aagagattaa cggcaaaaac tggacggagg taatgatgcg
     4861 ccccgatgta aggtgggggc actcggagcc cgatgccgat ccctgcggtt accggagcct
     4921 gatggtgctt cagcttgcag agaatcatta tgctgacaag gatctttacc aaagggccat
     4981 gaagcacccg gaaagggcgg taagacccaa ggccatagat ctggtggcaa tggtcgagag
     5041 tggagccatg gattacgcct tcgagtacaa gtcggtggct gtccagcatg aattgaacta
     5101 tgttgaactt cccgacgaga tcaatctcaa gaacccggat ttcagcgaaa cctacgcaac
     5161 tgcttctgta gaactggcgg gcaaggagcc gggacagaaa attaccgtca agggagagcc
     5221 catagtttac gggcttgtca tacccaaaac ggccccgaac aacaccggag ctctggatct
     5281 tgtcaaattc atactgaaga cgggaggggg tctcgatgtt ttccagaaga tggggcagga
     5341 tattgtcggc ccccaggcct atggcgaaac cgttaatgtt ccggccggga tatcggagct
     5401 tcttgtgaag taaaaaaaca ggaggaaaaa ggttgtcaag ggggcatggc cggaatttca
     5461 ggggccatgc ccccttgttt cagggataat ccagggggtt tttcgttttt gggttttgac
     5521 catggccaca cagagggtat cataatgtcg ggacaggtcc atgacatcgg caatatcact
     5581 gttcctgcgg agatactcag caagcccgca gactgtcgga actggagatg cagatcataa
     5641 gggatcatcc cctgcatgga agggatatcc ttatagacgt ggattccccc ctgccccttg
     5701 aagagattga taagaacagc ggtatcaagt acgatgcgga agtctcccga atatgcctga
     5761 agatcttcag ggagaagggg tattcgctgg cggactgatg ttcacttttt cagataaagg
     5821 ggcatgctct ctttgagacg atccgaaatt caagacgcag atattcttaa ggaaagtttt
     5881 tccagggatg gcttaaaggg gctgttcctt ccaggcagga tactcttctt ttccgcgggt
     5941 tttgtgctgc tttgggtggt ctctcgatac aactaccttc tttttcatgt tctggcggaa
     6001 ggtttttcca tagtagtagc atgcctgata tttgttctgg ctacaaggac ctacagattt
     6061 tcaggcaact cgcttcttct gtttctgggg aacgcctatc ttgcagtcgc gatagtggac
     6121 ctgttccaca cccttgcctt caagggaatg ggtgttttcc cctccaacga cccgaatact
     6181 gcgacccagc tctggatagc cgcccgatac cttgagtcat tctcgcttct tcttgccacc
     6241 tggctgggga acaggctgcc ccggaagatc cagttctggg gttttctggg tattgcatct
     6301 cttctggttt tcgttgtaat gagaacctca cttttcccgg actgtttcat cgccggagtg
     6361 ggcctcacaa atttcaagat agtctccgaa tacgttatct cggcgatcct cgtgacggcg
     6421 atgatccacc tctggcagat caggggtaat gttacgccgg tcattttctg gagcctgatg
     6481 ctgtcaatgg gcacgaccgt tctttccgaa atggccttta cgctttatac cgatgtttat
     6541 ggtgtgacga acgggctggg acatatcctc aagataatat ccttcatctt catttacaac
     6601 ggagtggtaa taagaggact cgacgaaccc tacggtgaaa tattcaggaa gctcaaggag
     6661 acatccctca gggatccgtt gacggggata tacaatcgtc ttggcttcat ggaatcagcg
     6721 gaaagatttt ttgcccttgc cagaagggaa aaattcggga ttgcgatgtt gatgatggat
     6781 attgacaact tcaaggccgt caacgacaaa ttcggtcacg ccgaagggga ccgggttctc
     6841 aaagattttg caggaatcct tttggactgc agcagaaaat ccgatatacc ctgcagactt
     6901 ggcggggatg aattcgtgat actcatgaag acgaatcccg ggggtgcagt catcatgagg
     6961 aacagggtcg aggcaagttt tctcgaatac cagaagaagg agtctcgact gggtataggg
     7021 ctcagcatcg gcgtggcctt tgcttctcca ggcgagtctc tctttgaagt cgataccatg
     7081 atgcgggccg ctgatagtga aatgtacggt tacaaacgca gcagatccca gaggtctgct
     7141 ccggggggag tctgagtgga gtttgaataa ggaaaagaac accaggaaac acctgaaaga
     7201 tattccgtct gtcctctccc gtctggacga gatcgagtcg gctgttcgag gaaggaatct
     7261 tttcgcggcc ctggattttg acgggaccct tgctcctctt gataacgatc caggagccgt
     7321 cgagatgacc gcggagatga aaaagaggct aaaaaacctt gccctcaggt gccctgtcgc
     7381 tattgtgacc ggcagggatc tcgaagatat aagggatagg gtgggactcg aagagctttt
     7441 ctactccgga agccatggtt tccagatagc cggccctcct ggaagcggtc tctccttcga
     7501 ggccggggga gagttcctgc ccgcgctgga taaagcagag aggcttctgc cagggatcat
     7561 cgaaggtgta ccgggtgcat tcatccagag aaagaggttc tccatatcga tacactacag
     7621 gatggtcagg cgggagcttg tccgggggct tgtggaaaaa acaggacgtg cagtaaaggg
     7681 tatcggcggg ctcgttgcga ggcatgacaa gaaatctgtc gaggtacttc cccgcatgaa
     7741 ctggaacaag gggagcgccc ttctctggat ccttgatgcc acagggatga cggaggcaaa
     7801 ttcctttgcc gtatccgttg gtgacgatat caccgacgag gacatgcacg tggcagtgaa
     7861 aggccgcggg gccgggatca tagttacggg aaccgagccg cccctttcta cctttgcgga
     7921 ctaccggctt ggcagcgttg ccgaagccgg caggttcatc gagatactcg agaggatcgc
     7981 cgggaacgtt accaggggcc aggaatctcc tgcaggaggt acgcaagcct ctcctccgat
     8041 cacttaagac aggagaccct caccctccgg cccataggtg ctatctcggt gacactgaga
     8101 ccggcactct cggccaatga ccggatctcc tccaccggca tgccgtgaga agggggaaac
     8161 cggctccatc aggcacaatc ttccaccggg aacaaggagg cgcgaaaggg catccattat
     8221 gccttgccgt ttctcgggag ctatgtcgtg aagaacatga atgaaaatga ccaggtcgaa
     8281 gcttccgggc cttagttcaa gagtacggat gtctccccgc agtatctcgg cattggagaa
     8341 aggagcaagc cttttgcggg ctctgtcggt catgtatgac gatgtgtcga ctccggtcgc
     8401 agagccgccc ttgccaagcg atttggccag gtaggtcatc aggacgccgt tgccgcatcc
     8461 gaactccagg actcgttcgt tcccttcgag tcccagtttc tccacaaagg gggcgtaaat
     8521 tatcggcccc gtgaaaaagg gatgaattgc tgccaggcag aatctgaaaa gcagtcccgg
     8581 tttttcaaaa tcccgctgtt cgaatttttc tccggataaa tggtctgtca aaaaaacacc
     8641 cccgagtgtc ttttggttta caatgatatt agcaatagtt catgggaaat ggcaacgcta
     8701 tttcaggatc acctcgtcac ctggtcttac tgttccgcct ttttcgacgg aaaggaagta
     8761 tccctccgtc ggcagaagac accatccccg atagttgtat gaatgaggtt ccccaggtct
     8821 tttccccttt tctgtgatgg agagcaggac cctgtttcct atcgacagtg tctgtcccgg
     8881 aaccagttcc tccgggagcc cttctattac gaggttttcg gccagagacc cgggaggaaa
     8941 ttcaaagccc gcctcttttt ccgccttgcg gatatcttct gccctcagga gacttacctg
     9001 tctttcgacc attccgaaat gggaatcacc ctctatacca ctgccctcgg aaaaaacagc
     9061 ttcctgcagg ggttccttgg gatctcttcg ttttccgctt gtacaaactg cgacgatttt
     9121 tgccaatgtg ttcagcctcc cttacctgat tactccatat tggactatta tagaaaataa
     9181 tacgggtcgt gtgttctgaa aggaggaata tgtcatgagg aaaaaggcta tcctcgcgtt
     9241 agcagttttg atgacactta tatttgtggt tccggattcc atttctgccg atacatccgg
     9301 ggttgcacgg ttttcctatg attttttcgg gctaaggttc gatgtagagt ttgtggatga
     9361 tgcggccgtg ctttaccttc cctcgggacc gttgaaactt ccccaggtga tttcgggttc
     9421 gggtgcgcgt tattccgacg gggaaacaac cttctggatc aagggtgacg atgcgtatat
     9481 ggagattaat ggttcagtgg ccgaagaagc ggattgaccc cgggaggtgg agggtttgga
     9541 tctttccttt ttttccgtgc ttgctgatat tgtaagtgtt gcaggtatat gcgtaatcct
     9601 ctggggatcg gggatcgcgc tatggcggtt tttcagggtc gagaagaaca tgttttcggg
     9661 aaagaacctc cagatgaaaa gggaaagtct gaggcacagt ttcgggagtt accttcttct
     9721 cggcctcgag ttcctcattg cggcggacat aatcaatact gtaatgaagc cgggtctgca
     9781 cgaggttgcc atcctggggg ccatagtggt tatcaggacc gtcatcggct acaccctcga
     9841 ccacgaactc tcatccgagg ccgacagtcg ccacaaacaa ggggagtgaa ggtcagggga
     9901 caagttccca ggaggaggaa gatcctggag tggaattgac gctgtaccat ttggccctgt
     9961 actgaaggcc gttatggaat atcgtgtccc cggcatagta cctgttggag ggaacccact
    10021 gatcggtaag ttcctgccag ggattaccca ctatacccgg ggtctcgttc tgggtccagt
    10081 accaggcctg gtactgtttg ccgtcgacca gaatgatgtc tcccccatcg tatatgttga
    10141 aaaatgtcca ttcatcggtg atctcttgcc aggggctttc gagaagaccg ggctgttgtc
    10201 cctgtgtcca gtatctggcc tggaataccc ttccgtcgta aataaccatt tgacctccaa
    10261 ggtagactgc accgttgtcc cattccgagt aatctggggt ttccggttct tcgcctccgc
    10321 ctggatcgtc accgggatcg tcccctgggt ctgttcccgg gtcatcgggg tctgagccag
    10381 atgccgtatc ccttatcagg accattacgg tatcatcggt gcttctgaaa ggggttccat
    10441 ttgtattgac cagttcgaat ttatttgctt ccttttcaag caatttgcgt atcgccgggg
    10501 agccaccgta ttcgtcgcct actctggccg ccgcgtacac cgcgccggag tcatcgggcc
    10561 tctgtatggc ataggtcctt gtcccgtccc cccaaccttc ggccagaaca tcccttatca
    10621 tgtaggagtt gagtttttcg tggtcgtcct catcgggcca ttcgcccgta tcgtagaaga
    10681 aaatcaccga ggcccgttta agggtcatga gatcggacac catacgggtg gcagtcgaac
    10741 ggttgcttgc gctgccgaac aacagcatca ggctgcccgc aaggatcccc acaacaacca
    10801 ttacaacaag gagttccacc agggtaaaac ctttttttgc gcggatcatt gtttaacatc
    10861 ccctacaaaa tgatgctgtc agtttaacag tagaatgtta ccccagaata aattttttgt
    10921 cactctgaat cgctggagtc ctcttcggtg aaggtcccgg ctcttatgcg gcctctgata
    10981 taatgaatcc cggccacctt tatggtgtcg gcggataatt atcgaatttc ttttgatcgg
    11041 gagggtttgc aatgatcaga ctggagacgg acaagggtga catactggtg gaactcgata
    11101 tggaaaaggc accgaagacc tcggaaaatt ttattaatta cgtcaggaag gggcactacg
    11161 acgggactat ctttcacagg gtgattgacg gcttcatgat ccagggaggt ggcttcgacg
    11221 agaaaatgaa ccagaagccg acggatgcac ctatcgagaa cgaggccaca aacggtctca
    11281 agaacgaggc ctacaccata gccatggccc ggacccagga tccgcactcg gcgacagccc
    11341 agtttttcat caatgtcaag gacaatatat ttctgaatca cacggccccc aacaccaggg
    11401 gttggggtta tgctgtcttc ggcaaggtca ccgaagggca tgatgtcgtg gatgccataa
    11461 agagggtcag gaccgggaac tgcggaggat atcaggatgt ccccgaggaa ccggtaaaga
    11521 taatcagggc atctatcgag ggggaggacg catgaaccgg gaggttcgcg ctgtagtgga
    11581 ccctttgctg gaggctgccg gtttcaccga ctgtctctgg gtgtctccat catcaatgat
    11641 cgtttcccag tgggtcagga tgaagtgtat gttcggatgt ccccattacg ggaaaaatgc
    11701 ctcctgccct ccgaatgttc ctaccgtatc tgagtgcagg gatctccttt cggaatattc
    11761 catggctgtc cttgttcacc ttgtctgtga tattccttcc cagggaaaga aagagtggtg
    11821 tcgaaccatg cagaaagaac ttctcgaggc agagaagcag atattccttg ccggcttcga
    11881 gagaaccttc cttctttcat ttgacaactg caacctttgc gatgagtgtg tgccttcccc
    11941 ctccgcatgt cttcaaccct gcttgcggcg gccgacgatg gaggcttttg ccatagatgt
    12001 gtactccagc gtgaaaggcc acggttacga agtgtcggtg agaagatctc ccgaagaccc
    12061 cgaagaccgg ttcgcgctga tcctggtcaa ataggagagg aaaatggatc ttcgctacgc
    12121 ttccgctata gcttctgaac ttggaataaa agaggaacag gccagacaga ccggattgct
    12181 gatagacgag ggttgtacag ttcccttcat tgcccggtac cgcaaggagg ccacaggctc
    12241 tctcgatgaa gttgccgtaa tggcaataag agatcgtttc tcgcagatgg aggaactgga
    12301 caaacgcagg atcgcgatcc tgaaatccct gggtgagcgt gacctgctga cggaggaact
    12361 ggaaaaggcg gtaaattccg caccgaacat gaccgccctt gaggatatct acctgccctt
    12421 ccgtcccagg cgccggacaa gggccaccgt ggcaatggag aagggcctcg aggaacttgc
    12481 ggtcctgctg atgaaggcgg cttcggggaa ccttgatccc ttcgatcccc tcgaagaggc
    12541 ggggcgcttt gtcgatttgg acaagggagt ttccgatacg gcggaggctc tctcgggggc
    12601 cagagatatc gttgccgaaa gggtcagtga ggaccgaagg agcagggtgg tgctgagatc
    12661 catatttgca aggaacggaa ggctttcctc cagggttatc cgtggaaaag aggataaggg
    12721 agcgaaatat cgggattact tcgatctgga ggagcctgcc cggtcggcgc catcacacag
    12781 aatacttgcc atgttccggg gagaaaagga ggggttcctc ggtgtatccg ccaggccgcc
    12841 ggcggaactc gcgatcaggg aactggaaaa gatcttcata aaggccgcca atggggcagc
    12901 cgaagaggtt cgtgccgcga taaccgatgg atatgaaagg ctcgcggctc cctccatgga
    12961 gacggaactc aagaatgctc tcaagttgaa ggcggacagg gaagccataa gggtcttttc
    13021 cggcaacgcc cgcgaagtgc ttatgtcccc gccccttggt tcttctcccg taatggccgt
    13081 ggaccctggc atccgcaccg gatgcaaagt ggttgttatc gacgcccagg ggaggctttt
    13141 gatggatgat gttatctacc ccttctcggg gaagaagcct ttcgaagagg cgtgtaggaa
    13201 agttgtcgat atcatggagc gttttcccgt ggatgctgta gccgtaggca atggtacggc
    13261 gggaagggag accgaggcgt tcctgaaggg gcttcatctt cccggggatc ctcccgtgat
    13321 actcgtcaat gagagcggtg cttccatata ctcggcttca aaagcggcaa gggatgagtt
    13381 cccagacagt gatgttacgg tcaggggggc cgtctcgata gggagaagac tccaggaccc
    13441 actttcggag ctcgtcaaga tcgaccccaa gtccataggg gtcgggcagt accagcatga
    13501 tgttgaccag aagatgctgg tgaggtccct ggaggatgtg gtggtatcct gtgtcaactc
    13561 agtgggagtg gacctgaaca cggcaagccc ccagttgctc tctttcgttt ccggtctggg
    13621 gccgggactt gcgggtaaca taatttcatg gagaaacaca aacggctcct tcagctcgag
    13681 atctcaactg aaagatgtac caagactggg gcccagggct tatgagcagg ccgccggatt
    13741 cctgagggta aggggaggag aacatcccct ggacgcaagc gccgtccatc cggaccacta
    13801 cgggattgtc ggaaagatgg ctgcagacct gggctgctcc gtggacgatc tgctcgcgga
    13861 cgaaactctg agggacagaa tagaaccgga tcgttatatc tgccccgagg ctggtctccc
    13921 tactttgagg gacattatgg ccgaattgtc caggccaggc agggacccca ggaagagttt
    13981 cgagacagtg aatttttccc cggacgtaaa gaagatcgag gacctcgtac ccggtatgga
    14041 actggatgga gtggtcacca atgttaccgc tttcggggct ttcgtggaca tcggtgtcca
    14101 ccaggatggt cttgtgcatg tcagcaggat ggctgaccgg ttcgtaaaaa atccctctga
    14161 tgtggtgaaa ccaggccagg gggtcaaagt atccgttatc gaagtggaca ttccccgtaa
    14221 acgcatatct ctttcgatga ggaaagacaa gaaagattcc gaacgacctg ggtctgactg
    14281 ataccgagaa ttgctgatac gctcggcctt gttctctgag atactcagta acgggtgatg
    14341 cgtatgaggc cgagggtctt tttagtttta attgtttctg tggctcttct tttcatcaaa
    14401 gggcaggcga gggctgacgt ttttcaggtt caggtactga gcctggaaga gtgtgtcgag
    14461 atcgccctct ccgggcaccc ggatctggcg gctgcaagat ccgacctgga ggcttctcgc
    14521 gcaagggtcg ggcaggccag gacacaggat aacccgtcgc tggcctattc tacttcctac
    14581 accgaaagag gttcttctct ccagggcagt tccggcggtt tgtggtcaag cagtttgaca
    14641 ctttcgcaac tcgtcaccga ctggggcaag actcactctt ctgtcaaacg gtccctctcg
    14701 gaacaggaga tatcgtctct ttctctgaaa caggcccttc ttgacgtcga atatgatgtc
    14761 agaagggctt attttctgct tctgaaagcg gaaaagaacc ttgaggtggc aaaggaaacg
    14821 atgtctatca atgaactcca gcttgaaaag gccgaggctt ttttcgaggc ggggcgggtt
    14881 tccaggtacg acgtgaccgc tgcccaggta tccagaagca acgctaacat ggcgctgatc
    14941 caggccagga cctctcggga caacgccatg tcggatcttc gaaaagccat gggttttaca
    15001 ggggccgagt tcggtgtgtc agatccggaa ggcctgtccg agatgacaga gaataagaac
    15061 ctcccacttc tggaacaggc tctgcaggca gcctacgaaa aaagacccga ccttctatcc
    15121 tggaaaaaca gggtggaatc ggcgagattg tcagtgacgc tcgcaaagct ggacaatgct
    15181 ccaaaactct ctctttccgg cagtttcggc tggggttcat cttccttttt cggcgatgag
    15241 gactggaggg gggctttgac cctttcgttc tctctttatg atggagggct tcagaaagag
    15301 aagacccgtg aggccctgtc ttccatgcag gcggtggaag caaggtgtga atctttcagg
    15361 cagactgtgg ctacggagat cgaaagcgct ctctcgaggg cccgggattc ggaaagcgag
    15421 atcgaagctg ccggggaggg cctgcgactg gctggggaaa accttgagat agctacagga
    15481 agatatcagg tcggtgtcgg gtctcccatt gaggtatccg atgctgcaag aaattatatt
    15541 gatgcgaaag tggcctggca ttcagcgatc tacgacagga tgatcgctct tgccgcgctg
    15601 gaaaaagcga ttgggggcag caggtgatga aaaagaaatc tgtgataatt gcctttctgg
    15661 tccttgcggc ggcaggaggc gcgctgtatt tgcgttttgg cgggaaagat acccctgttg
    15721 tccagaccac cccggtgatc cggggggata ttgtaaggtc agtctccgcg accggtcgga
    15781 tacaggcagt caacaccgtc aacatcgggg cccaggtgtc aggcacgatc agggtgatcc
    15841 acgtggatta caattcggtg gtaaagaagg ggcaactact tgcggagata gatccctccc
    15901 ttctggaagc aagcgtaaga caggcccagg caagcgtcag gtccgcgaag gcatcccttg
    15961 cagaggcaag agcggttctc gatgatgcgg caaaaaacct gaggagaagc gaagatctct
    16021 tttccagggg ttatatctcc aaaaccgatt ttgaatcggc gcagagcacc cacacggcgg
    16081 cactggccag ggtcgagacc acaggggcaa gcctggccca ggccgaggcg gaccttatgt
    16141 acaaaagcat caatctgggt cacacgagga tcgtctctcc aatagaaggt gtcgttatag
    16201 acagggctgt tgaccagggt cagacggtca atgcaagcca gtcagctccg accctttttt
    16261 caattgcgga agatctttca agaatgcagg ttgaggcttc catagacgag gctgatatcg
    16321 gcctgatatc acgtggccag acagcaaagt tcaccgttga cgcttttccg gacgtggatt
    16381 tcgagggaac cgtcagggaa attcggctct caccctccac aagcgagaac gtggtcacct
    16441 acactgtcgt gatgggtgtg gaaaaccacg aactgaaact aatgcccgga atgactgcaa
    16501 atgtcaccgt cattgtcgaa tccaggaacg atgtcctcaa ggtggccagt tcatctcttc
    16561 gtttccgccc gccaaatgcg gaacagcagc cacggaaaca gggggggcag aacggccagg
    16621 ggcgcggtgc ggcgatatgg tcgttcgagg aaggaaaact ggaacgccat tcagtaaagc
    16681 cgggtatatc cgacggcatg tatacagaaa tatcaggaga tattgaagaa ggaatggagg
    16741 ctgtgaccgc tatagattac ggttcttctt cgagtggagg gggtgccgcc tttggaattg
    16801 ctccgcgcag ataatctttc aaagacatac cttatgggcg atgttaacgt tcaggccctg
    16861 agcggagtca gtttctccgt aacgcgcggg gagttcatct cgatcatggg tgcttccgga
    16921 tcggggaaat ctaccctgat gaatattctc ggttgccttg acacgccaac ggacggtaaa
    16981 tacatactgg agggaaagga tgtcagcgga ctttcccgtg atgaactggc ggatatccgc
    17041 aaacacagga tcgggttcgt ttttcagggt ttcaatctcc tgccgaggat gtcggccctc
    17101 gagaatgtta aaatgcccct gctttacaat gaaatcccgg cccatgaaag ggatcccagg
    17161 gccatggagg cgctggaaat ggtcggcctc ggtgacaggg cgaatcacca gccacagcaa
    17221 ctttccgggg ggcagcagca gagagttgcc attgcccgcg cgcttgtgaa cgggtcttcc
    17281 attattcttg cggatgagcc tacaggaaat ctcgacagcg aagcaagcag agaactaatg
    17341 gatctttttt cgagactgaa ctcaaaactc ggaaggacca ttgtccaggt aacccatgag
    17401 caggatatgg ccgcgtacac aggaaggatc atctctttca gggacggcag gatagtcagc
    17461 gatagcgggg tcgagagaaa atgatagccc tgtccgagac aacaaaaatt gctttcaggg
    17521 cgctcctggt caacaagacg aggtccgcgt tgaccatgct cggaatagtc ataggggtaa
    17581 tggccgttat catgatgttt gctattggcg ccggagcaaa caggcagata gccgaaagat
    17641 tttcgagcat gggaagcaac atgataattg tacgtcccgg ggccagccag ggtccggggg
    17701 gtgtttcggg aatatcttcg aggaacctca ctatcgagga tgcctatgcc atcggttccg
    17761 aatgcccggc ggtggcctat gctgctcctg tttccagtgg aaacgcgcag gtcgtttacg
    17821 ggaaccagaa ctggtcggcg cctgtaactg gcactacacc ggactatctg ttcgtcaggg
    17881 actggggcct tgattccggg cattccttca cggagcagga tgtccgaagc gcggccaaac
    17941 ttgcgatcat tggcaagact gtagcggaga atctcttcgg gaacgaggac cctgtcggga
    18001 agaccataag ggtactcagg gtgccggtgg tagtcgtcgg ggtccttgga gagatgggcg
    18061 agagtgcctt tggacgggat gaggacaaca taatactcgt tcccataaca actgcccaga
    18121 gaagactttt cagtacatca agaatcggtt cggtttcaag cataatggtc aaggcaacgg
    18181 ggagcgggat gctggaggct gcccagaagc agatcgagat gctcctcgag ataaggcaca
    18241 gaatacggcc cgggggggag caggatttca gcgtaaggaa catgtctcag atgatagaga
    18301 acgcaaagag cgccacaagg ataatgaccc ttctccttac ggctgtagct tcagtctctc
    18361 tgctggtggg aggtatcggg attatgaaca taatgctggt ctctgttacc gaaaggacgc
    18421 gggagatcgg aataaggatg gcacttggcg caagaacagt tgatatacgc cttcagttcc
    18481 ttgtagaggc agcggccctt tccatgctgg gcggttttgt cggtatcctg ctggggataa
    18541 caggttcgca ggtcatatcg agaggctttg gatgggccgt tgagatatcc cccttttcgg
    18601 tggtcctggc tttcgggttt tccgccctgg tgggtatttt tttcgggtat ttcccggctg
    18661 tgaaagcctc catgctcaac ccgatagagg cgctaagata cgaatagggg agcggagaaa
    18721 tcccccgccc ccctatttat tcccgaagaa tcggattctc tagaacttca ggcccgtcac
    18781 ctaccaggtg ttgtgggcgc ttttgagtct gatccgcgtt tctacgatct ttccatcgta
    18841 tgtctcggcc ttcaggaaat tgccttctgg aaggcgccaa tgcggcaccc cacccatgaa
    18901 gacaagccga acaggattgt ccttcataac ttgaaacctc gtttcctttc aatattgtgg
    18961 atacatgata ccattcaatt tcaatgatgt caaaaaggaa gcactacggg aacaggcaaa
    19021 ctgaaagaaa acagtatggg gctgcaacct tcaacctgct gatcacagat ggctgattca
    19081 ttctgatgga aagatgagtt atcatatcag ggagcttatt acagaggtga ggtcaaaaaa
    19141 ttatgcttaa agagcccgta attatctatg tcttttcagg atccggtaac acctggactg
    19201 tcgcaagaaa gatggcagat gaacttctcg aatgtggggt cgcaacggaa ctgcgcccgt
    19261 tgcgacggga aggcccgggg gacatttcca gggccgggac tttggggatc gctttcccgg
    19321 tggccatgtt cacggcttat ccgtttgtgt gggagtttgt cggcaatctt cccgacggaa
    19381 aaggaatgga tgtgttcatg gtggataccc ttgccggttt cagtggaggg gtcgttggcc
    19441 cgatgcggga tgccgtgtcc gcgaagggtt tccggcccat cggggcaaaa gaagtacgga
    19501 tgccttcaaa ttacgccaga acggacccgg gaggggagaa agataacgag aacagaacca
    19561 gggggcttaa caaggcggcc agctatgcca gggatctttt cgacggcaag acctcctgga
    19621 ggagagttcc cattgttccg gaccttgtaa gggctgtagg caaaaaccct gacggttccg
    19681 cttggagatc gatacgtcga aaattcccgc tggaagtgaa tagcgaaaaa tgcacccgtt
    19741 gtggtctttg cctcaagttg tgtcccgtcg gcaacataac catggaggag attccccgtt
    19801 tcggcaaaaa ctgttttttc tgccagagat gcatttcttt ctgtccagag gaggctatat
    19861 ccgtttccgg caagaactat gttccctaca ggggaatgga taaaaaaacc cttctggagt
    19921 ttttgggaat atagggaacg ggatatcaga tatctgcttt cttccgatga tgcgtacagc
    19981 cttggagcgt gatataattt cgcctgttga atcatcattt tgtaatgtct ctgcgatgct
    20041 gtctccatag ggtcgtcctt cgtgcgccct gtcaaggagt ggctttgata catagggaaa
    20101 tgaaaggagg cgactctgaa tgtccgaaaa atggaaagat gaactgaccg aacagctggc
    20161 aaaggcctta ctgactctga aaaccagcga ggaggtctac gacttcctgg aggatgtcgc
    20221 aacaataggc gagatccgtg ctcttgccca gcggctggaa gtggcaaagc gccttaacga
    20281 gggtgagact tacccggtta tcgcacagca gaccggcgcg agtaccgcta ccataagccg
    20341 cgtcaagaag tgcctggatt acggtgccga cggttacaag aacgttctgg acaagatgaa
    20401 ataggcggtc tcccgtgcat gaagagatcg ggcattccgc ccatatgcgg gatgcccgat
    20461 tttcataaag aggtggtggt cccatggcaa gtcttttccg tttctttgct tttgctcttc
    20521 ttgtgatcct ttctcttgtc gcctgggctt tttttgatta ttcaaggaac aggaggaagg
    20581 cggcctctta cttcagggaa cgtctaggaa tggcaaaaga tctgtttaca atgaccaggt
    20641 tcgtaaatat ggcacgtgtt gtaattcccg agaccgaacg ctttattgcg gtttttttca
    20701 ggaagggaga gcatcttgag atcagggatt ttgcccccga ccgggtgatc gacatccctc
    20761 cggattccgt tctcctctcg gatagcagaa ggaacctgac gtggatattc gtcgaaagaa
    20821 gtaagaccat cttttccctc aaggtcacgg attttttacc cgggaccctc tgccatgtaa
    20881 ggaaggggac gggcagggta aggttcgggg acgaggacat tccggccagc aacagggact
    20941 ggttccttat ggacagggag agaggcgttt ccttctcgct tcccaatggc aaggaaaaag
    21001 acaaagaaga gggttttttc ttctacgacg ggtttgctcc taccgaaggt ttccttctgg
    21061 atgaagatgg gggagttctt ctggttgacg aaaaaagggg tacctttggt ttcagggagg
    21121 ttgccgggga agcattgact ttgtacaggc ccgatgacat agtttctgcc gctccgtccg
    21181 tggagggccc ggattttgtt gatataaccg tcagggacgg taaaaggcca aggttcacct
    21241 tcgagtttga cgatgagggg gaggcccgtt actggatgga atggttcgtt cgggtcaggg
    21301 atgagatgtc tggaaaggga acaaggacag ggaccaggtt tgtgaggata tccccagtca
    21361 aaatatccta gggggcctgt ctggttgaac cctttttggg agtatttccc tttctctctt
    21421 tgtggcggat gaatattgcc ttgttgcccg actcgttgtc aacggctctg gcttccacct
    21481 cgaagaggcc tgtggccttt accagggaac tcagtttcct gaatccgtaa tttcgggaat
    21541 cgaacgaggg tgactggttt gaaatgtgtt ggcccacgtt tccgagagct gcccatccct
    21601 cctcgtccgc gactgcatct acgctgttgc gaagcaggtt cgtcagtttg ctgtcacccc
    21661 tgagcagtct tgggctttgg ggttttttgt ttgttgatcc ttccccgatg gtatgctcct
    21721 gttcctcttc ttcctcatcc ttgccgagga tctccgtata tatgaacttg tcgcaagcgc
    21781 ttacaaaagc gttgggagtt ccttttttcc cgaatccgaa aaccgttatt cccgcttctc
    21841 ttatcctggt agcaagacgt gtgaagtcgc tgtcacttga aacaaggcag aaaccatcaa
    21901 aacggctcga ataaagcaga tccatagcat cgattatcat ggcgctgtcc gtggcgttct
    21961 ttccgacagt atagtgaaac tgctggatgg gatgtatcgc gtaggacagc agcacttcct
    22021 tccacccctt gagatatgga gatgtccagt cgccgtaaat cctttttacc ctggaaattc
    22081 cgtattttgc gatctctgcg aaaaggttgt cgattatgga aggagaggtg ttgtctgcgt
    22141 ctataaggac cgccaggttt tgctgcttgt cagtttccgg gatcattcta tttccccctg
    22201 tatgtttttc atactaaagt cattatagcg catcgcgggg cccggaaagc atggattcga
    22261 tctttttgag gatgctgtcc agcactaccg caaaaaatgc ggccgatatt gctccctgga
    22321 ggacgattgc ggggttctgg gagatcagtc ccgaaataat tggtgccccc agccctcccg
    22381 ctcctattgt tgcgcctatc gtcgctgttc ctaccgatat aaccgccgat gttcttatgc
    22441 cccccatgat taccgggaca gcaagaggta attccacctt gaggagtgac tggaccggct
    22501 tcatccccat ccccgcggcc gcgtcaagta tgtcggggct tacggattcg agcccggtaa
    22561 ctgtgttgcg gagcaccggc atcagtccgt agataaacag ggcgaacaga gccggctgga
    22621 agccgaaccc cagggaaggg accgccaggg ccaggactgc cactggaggg aatgtctgtc
    22681 ctatcgatgt tatgtcatcg accaccggaa tgaagtccct gccgcttttc cttgtgatca
    22741 ggatgccgat gattactccg aggatggtgg ccagcaggga ggagacaaga accagtacca
    22801 ggtgttccca cgccagggtg atcatgcttg aacgctcgta aacaacctgg gcccgggatg
    22861 ggaaaagcat gcgaaggatc ttctcccaga atggttccgc caggagcatg gccgtgtatc
    22921 cggtggccag aagaacagga agaataatcg attcacggcg ggggtgggag gtcatcgtgt
    22981 ttctccaggc ccaaggatct cggcaagttt cctgaatccc cagtcggcgg tgatggcaag
    23041 catgaccgtt ggtatggcac ccagaagaat aaggtccggt gcggcttccc cgaggccctg
    23101 gaatatgaac tgacccatac caccggctcc gataagggcc gctacagcag tattccctat
    23161 agcctgaacc ccggatatcc tcaccccggc gagcacgacg ggcatggcca atggaagctc
    23221 aaccctgaga aaaagctgtt ttttgctcat tcccatcccg gaccctgcat cgacgatgct
    23281 cgggtcaata atggaaaagc ctgaaacggt attttttatt atagggagca gggaatagat
    23341 cgtcagggca aggaatgctg gagcccagcc tattccgcct acgccaattg aagcgaggga
    23401 agggaaggat ctcgcgaccc acgccagggg cgcaatgagc agcccgaaga gagccaggct
    23461 cgggatcgtc tgaagtgtgt ttgctaacga aattatcgcg ctccctagtc ttttgtttct
    23521 caatgcgacc agtcccaatg gaaaccctgt aagcagaccg gccgaaacgg ctgaagatga
    23581 aagggcaatg tgcctcgcca gttcctgtat gaaacgcccg ctgcgattgt ggtactcctt
    23641 caggaaggaa atgttgtcca ttccccctga aaaaaacagg aaggccaggg ccgcataaaa
    23701 cccccagcaa aagaaaacga agagcttctc ctcgcgagcc gccctgcggc agggggacat
    23761 gatcatgaag gatccaagga gggccagcca tgctcccgaa ccgatggaga ctctcgcgaa
    23821 tggcatagct ggcaccgaga tcctcgtcgc ggaagctccg gcttgccaga ggatggcaag
    23881 aaaaaggata cgggaaaggg tcgccgaagc gatgtttctt cttcttgccg gaccctttcc
    23941 cgcaagaaaa aatgcggcca cggcaaggga caggatcaca gccacgggga aaaaccccat
    24001 ggcctcaaca gcacccaccc catgaggcgg ggagatgcgt gtctccctga caacgacaaa
    24061 ggggttcagg aacagggggg ccataacaag cagggaacca gccgtaatga ccctgtcgaa
    24121 tccccgggga gagatttctg ttcccctgga aaagaggccc attccctatt tcaggagccc
    24181 cagggacctt agatggtcct cggcgacccg ggctggatcc tgtccttcga cggctatcct
    24241 gccgttaagt ttctggagcg tcaccaggtc cagggtgctg aacacagggt tgaggatatc
    24301 cgctatcact gggttcttct ctatcatttc ctttctgatt atgggggcgg gttcgtaaac
    24361 aggctggact cccattgaat cctccagaac aaccagatcc atgaccgcta tgccgccgtc
    24421 agtaccgtat gccatcgccg cgttgacgcc gtctcttccc agcgatgccg cctgttcggt
    24481 ctgtgctgtg ttgccgccgg atagtatcag aaggtcttcc ctgcccagtt tgaagccgta
    24541 cgccgattcg aaggcaggaa gtgcatctgg cctggtggca aactcctcgg aacacgcaag
    24601 cctgatcttt cccccggcgt tgatgaaggt cgcaagatct tcgagactgg aaaggccctc
    24661 cttttcagag aggtctttcc gtacggcaat ggcccaggtg ttgtttgccg gtgcgggctg
    24721 aagccatact atcccgttct tttcaaggtc cagggtagag actctctcga aagccttttc
    24781 ggggtttttc catatctctg tgtctgaatc gtcgaaaaaa tagtaaccat ttcccgtgta
    24841 ttccggatag atgtcgatct ccccggcaat tatcgcctgt ctcaccacgt ttgttgagcc
    24901 aagggatgtc ctgtcgttta cactgaagcc gttttcgcgg agcagcagta ctatcatctg
    24961 gcccagaagg ccgccctcgg tgtcgatctt tgatccaaca atgatctttt cctgtgcgct
    25021 tgatgcttgt ggtgccgata atggaagcgc cacggcaagg agaaccgcca aaagtgccgc
    25081 gatcgcaaga accctgcccg aagatcttcc tttgtccttc atggtgatac ctccctgatg
    25141 aatcgattca acacattaca gacaatacta gttactttac gacatcttgt caaaaagggg
    25201 gtgtttacag tgcaggttct ggaacctata cttgcggaac gaatagaatc ggcaccagcg
    25261 ggttactgaa gaattcgggg caagaccagt atgccttctg cgacaagatt gttttttgaa
    25321 aggatcttta agtatgacct tttcggcact ggcttcaacg gctgttttcg cggcgacgat
    25381 cattgctatt gccatgggca agatgcaaag aagcgtagcc tctcttctgg gagccagtct
    25441 tctcatggcg tttcacgtta tcccggcgga aaaggcggta acgtatatcg atttcaacac
    25501 cataggcctt ttgatgggca tgatgataat agtcggggta cttgcccgca cgggtgtgtt
    25561 ccagtatgtg gcggtcaaaa ccgttaaatt taccggaggc aatgtccggc tcacatttgt
    25621 ggctgttatc gctgtaacgg cattcttgtc tgcctttctg gacaacgtta cgacggtgct
    25681 tctgataagc cccgtggtca tatccctcgt cgaccttatt gatgtcaacc ctgttccctt
    25741 cctgctcggg gaagcctttg cctccaatat tgggggagcg gctacgctgg tgggcgaccc
    25801 gccgaacatt atcataggtt ctttcgccgg tttctctttc atggacttta ttctgaatct
    25861 gactcccgcg tctgttcttg ttctggtgat agtctgcgga tggcttctgt tccatttccg
    25921 caaggagttt accgtttcgg agacctcggc agaaaggata gcccaggtcg atgagagcag
    25981 gctgatacgt gaccccctgc ttatgaggag ggcgtcgggg gccatgcttc tggtcctggc
    26041 gggtttcgcg gtccaccacc tgatacatct ccctgcgtca gtggtggctc ttcttggcgc
    26101 atctcttctt ctcgcggtag tcccggtcag tggcgacgaa gtcgtccacc gtgatattga
    26161 atggcctacc attatttttt tcgcatccct ttttataatt gtaggagcgc tcagggagac
    26221 tggagtaata acggccgtat cgaacatcat ggctgccatg ctgcagggac atccttttct
    26281 ggctcttctt gcggtactct ggttctcggg gctctcatgc tgtttcataa acaatgtggc
    26341 tttttcggcg acctttgttt ttgtcgcaag agatctggcc ttgtccctgg gtatggcggc
    26401 ctcgcccctc tactgggccc ttgccctggg cgcttgcctg gggggaaatg gttcattcct
    26461 gggttcggcc gcgaacgtta tagtcgcgga catggccgaa aagggaggaa gcagaatatc
    26521 cttccagacc tttatgggtg cgggtttaaa ggttgttctg atatcacttt cggtagcaag
    26581 tgtctacata atgatcaggt gggttttgct gggtggctga aatagttcct ttacctgggg
    26641 aaacacagat aggatacttt atcaaagaaa gaacgctgga ggtgtcggga tgcttgttga
    26701 agagataatg gaccgcgacc tgacggctgt ttctcccgat acgactgttg cagaggtcgt
    26761 ggagatactt tctgcccaga gaatatccgg agttcccgtc gttgatgacg agggccgcgt
    26821 cataggtttc ctgagcgaaa aggatattgt caaggcagcg ttgcccgggt actttgatct
    26881 gcttcaggat tcatcttttc ttcccgacta cggacagttc accagacgtc tcagaatggt
    26941 tagtcttgac agtgtggata aatacatgag gaaggatgtc atcgtcttcg acgaaaagga
    27001 taccgatctt catgtggcga tggtcctgat caccagaaat ctcaaaaggg ctccggtcgt
    27061 cagggatggc ctttttgcgg gagtgatcag cagggctgat ctgcttgatc acattctgcg
    27121 ttccaggaag gagccgtcag attgacaagg gctggtcgaa ttgaattgag gtttcatggg
    27181 ccgtggctga gggtcgcggg aaagcccaca atgacgtacg agatcgatgg gcctgttttg
    27241 ctcgaggaac ttctttcagc gttggaaaaa atatttggaa gcgggttctc atcccatgga
    27301 gaaggcctgg tgtgtctgct cgaggattcc gggccgagag ccctgaaaat gaagaccccc
    27361 gttttacccg gttccactct cgtttttgtg gggaccgtcg aaagcgggta agatcaggga
    27421 cgggaggtat tgtgatgaat ggataccatg gcaggatcgc ggttgtagac cttgcggcga
    27481 aaaggtcgag ggtaatgcgt tttgacgaag atttcgcgcg aaaatacatc ggaggatcag
    27541 gtatggggac ctacctgctt ctgaccttct gtaccccggc cgtgaaaccc ctttctccgg
    27601 cgaaccctct gatatttatg acaggacctt tcatgggaac ggggatacct acctcgggcc
    27661 ggcaccaggt gctgacaaaa tctcccctaa cgggaatctt tctggaatcc gacgcgggag
    27721 gcagttttgg ttttcacctt aaaagaagtg gtttcgatgg tctggtcatt gtcgggaaag
    27781 cctcggatat tactgttctg atcctgaggg acggacgggt cgaattcgaa gacggcaatg
    27841 attttagggg tctggacaca atggaaaccg acgcgaaact cagggggatc tatgggaaaa
    27901 acatgggcat atcatgcata ggccccgccg gggagaaaca ggtccctatc gcgggaatat
    27961 tccatgatgg aaccgacgca agaacttcag gcaggggtgg agccggagcg gtaatgggca
    28021 gcaagaacct taaggccata gttgccgggg gtagtgaaat ggcacccctg aaaaaccgga
    28081 aaaaacttgg tgaacttata tccatcaaga attccaccct caaggaaaag ggctcgggcc
    28141 ttactctcta cggaacatcg ggggggatgg ggctttgcga ggaactgggc gaccttccga
    28201 tcaggaactg gaaacagggg tcatggaagg aggaagtcaa ggctattacc ggacagaaaa
    28261 tggccgaaac catcctttca ggcaatttcg gctgtgtcgc ttgtcccatt cgctgcggcc
    28321 gggtcgtaaa gatagatggc cgtgaagtgg ggggccccga gtatgagacc atggccatgc
    28381 ttggaagcaa ctgccttgtc gatgatctgg acgcaatagc gagggccaac gaaacatgca
    28441 accggctggg tattgacacg atctccgccg ggtcggcggt ggcctttgcc atggagtgtt
    28501 ttgagaaccg cattatcagc gaagagcagg cgggaatgcc tcttgaatgg ggaagcggag
    28561 atgccctgaa cttcctcgta ggtaaaatgg gcagaaaaga gggaataggt gcacttctgg
    28621 ggaaggggac aagggctgcc gccagggaac tcggaggcgg ttccgatcgc tttgccatcc
    28681 actgcaaggg tctcgaattt ccggctcatg acccgaggtg tttcaagagc ctggccgccg
    28741 gttacgcaac ttcgaccagg ggtgcctgcc acctgaacgg ttattcatat gcctgggaga
    28801 gatccgcttc gttccccgag ttcggataca acgaaaaaca ggaaagagga actgacgaag
    28861 gtaaggggat catgaatgcc cgtttccaga acctttccag ccttgccgac tcgctgaaga
    28921 tttgcaagtt tccgttactt ctgggtgttt ctctttcgga tatttcggaa tggatgcagt
    28981 gtgtgaccgg ttgggatatg gaccagaaag aacttgaaag ggcgggagaa aggtcttaca
    29041 atatgaaaaa actattcaac gtaggccatg gtctttcgag gaaagacgac attcttcctg
    29101 aaagaattat taaagagccc aggggaagcg gaggttccgc ggaggctctt cccgacctgg
    29161 aactccagct ggatgagtat tactcatata gaaaatggga tgagaacggt ctgccgaccg
    29221 tggaaaaaat cgaggaactg ggccttcgtg agttcattca ctggcttgtt ggctgaacag
    29281 gtgtatgata gttgttgaca tcgtgaaatg aaggtataat tacagatgaa aaattgctac
    29341 atggtgaaca gatgcctcgg gtgatcacga acagcatcac ggatatcaca gggagggcga
    29401 tataccatgg aggtacttaa agtctctgca aactctcagc caaaatcagt ggcaggggcg
    29461 atcgcggcgg ttgtcaggga gagcggtgcc gtagaggttc aggcggtggg agccggtgcc
    29521 gtgaaccaga cggtcaaatc catagccata gccagaggtt atgtttctcc caatggtatt
    29581 gatctggtct gtatcccgtc ctttgccaag atcgatattg acggggaaga aagaaccgct
    29641 atcaaattcc agctagagag tcgctaaata gcctttttct ttaatctgta attttttctt
    29701 tacgggccgc ggataccgcg gcccgtaatt ttttgttttc ctggatcgta accagaatat
    29761 cgaaggaacc tggaaccgag agatcgtaat tccatcatcg gcaattaaca gactggggta
    29821 atttggcgtc cgacagctgt ggattcggac aacttgtcac gagtcacaag attggagtta
    29881 cggggcagtc tctgcttccc cgtttcaaag actgcttcct ggtgctacaa tcagacgatt
    29941 ccaatccggc aaggcgtaag ggaggaagtt tcatgtccag cataagtgct ccgcgaggcg
    30001 taagggatat tctccccgat gaatcgtgga aatgggagca tgtacttgat gttacagcaa
    30061 gaacagccaa agatttcggt tacagggaga ttcatctgcc tgtattcgag cataccgaac
    30121 tcttctccag aggtgttgga gaaacgaccg atgtcgtcga gaaagannnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30241 nnnnnnnnnn nnnnnnnnnn nnnnnnaggc agcctcttct ggatgccttc gtggtgtgct
    30301 ctgaagaggg gatccgggac gaagccgtca agcttctcta cgaactgagg agtgctggta
    30361 ttgctgccga tattgactat tcgggcagga gtttcaaggc ccagttcaag tttgcgggtg
    30421 gcagcggcgc ctctttcgcc tgtgtaatgg ggcctgagga ggtatcttcc ggatctgtta
    30481 ctgtaaagga ccttgaaagc ggtgaacaaa ggcgggtagc ccggggagat gttgcttcgg
    30541 cgataatggc atctggacat aaaaatggct gatgacagga aggaaagata agacagaatg
    30601 aaacagcgtt tctttgacag cacgtggaaa agaagttgtt actgcgggga tattccgctt
    30661 ccctgcgaag aagggccgga agtagtaata aacggatggc tcagacgccg aagggatctt
    30721 ggcggcataa tattcctgga gatctgggac cataccggag tagtacaggt ggtattcaac
    30781 cccgagcttg ggcctcttta cgaaagggcc aaaagcctca ggaatgaatt tgtgctggcg
    30841 ataaggggca gggtgagaaa gcgtcccgaa ggcactgaga atccttccat gccaacagga
    30901 gacagagagg tagtggccag ggatttcctt cttctttctc cgtccaggca gcttcccttt
    30961 gaggtttccg aggcggacag ggtcgacgag aacctcaggc ttaggacgcg ctaccttgat
    31021 attcgccgcc cggtaatgca gaaaaacctc aggaccagga gcaaggtggc ctcctttacg
    31081 agaaactttc tttcagggga gggttttgtg gaggtagaga cacctatgct taccaaatcg
    31141 acgcctgagg gagcaagaga ttacctggtt cccgcaaggg tgatgccggg aagattcttt
    31201 gccctgcctc agtctcccca gatattcaag cagatactca tggtaggcgg gatagaccgt
    31261 tactaccaga tagtaaagtg tttcagggac gaagatctca gggctgacag gcagccggag
    31321 tttacccaga ttgatctgga aatgagtttc attaccgaag aggagatcat ctccctgatg
    31381 gaaaagttca tgaaaggtct tttttccgat gttagcggga tcgacctcga aacacctttc
    31441 ccgaggataa cctggaggga tgccatcagc agattcggga gcgacaagcc ggatctcaga
    31501 atagattcag agatactgga tctggataat gtgtttgcgg cctcatcctt tgaaccattc
    31561 ataaaaaccc ttgaacaggg cggatctgta aggggattgc atattccggg aggggcctcg
    31621 ttgtcccgca aggagatttc cgatcttgaa acacaggcaa aatcatttgg agcgagtggt
    31681 ctggctccct tctcaatgaa ggatggagtt ctcaaaggtc ccctggtcaa atttctttcc
    31741 gaagatcttc aaaagaccct ggttgaaaac gttggactta atgaaggaga tgctcttgtc
    31801 gtagcggggg gggcatggct ggagacctgt gaggccctgg gtcatctcag gctggaactg
    31861 gcggacaggt tctacagatc ggatgaatca aaatgggcct tcctgtgggt cacggagttt
    31921 ccccttctcg agtgggatga agagggatca aggtggtcgg cggtccatca cccgttcacg
    31981 tctccgaacc tggaggatct ggagaaactc tcggacagcc cgggaaacgt caggtcccgt
    32041 gcctatgacc tggtccttaa cggtacagaa gtgggtgggg gttccataag gatccatgat
    32101 cccgaggttc agcagaagat gttcgaggtc atggccttca ctcccgaaaa ggcccgggaa
    32161 cggttcggtt ttctcctgga tgctcttgct tcgggaactc cgccccacgg tggtattgct
    32221 attggtttcg acaggcttgt aatgcttatg tgcggtgtcc actcgataag ggatgtcata
    32281 gctttcccca agacccagaa ggctcagtgt ctgatgtcgg gagcaccatc tgacgtggat
    32341 cccgttcagc tatcggatct tcacattgct ctcgatatac gtggggagga agaagaatgc
    32401 gggtcagggg gcgcggaata gatatcgatg gcgagagaat gccgtggtat tacgatctgg
    32461 aagagtccgc cggagaattg aagggccaac tgaaggtaga gggctgggac ccttcagccg
    32521 ccatgaattc ctggtacgaa agatcaaagg gcaagaagat cgggatcatg attccggggc
    32581 ttggaagggc tatcctgagt cccacgggga tattcattca tgaatcaggc catcacaatg
    32641 agacggaagt tatagtaagg ggctcgctga aaactgcctg aggcggctgg cgggcccttt
    32701 gcctgttata atccgggtat catggtagca ggggaggaga ggctatggca aggataaggt
    32761 ttctcggaca ctcggccttt catatcgagg gtgagggtct caacggactg gttgatccat
    32821 ggctttccgg caacgggatg tctccctttt cgccggatga ttttccggaa gtcaacttta
    32881 tcttcgttac ccacgggcac ggcgaccatc tcggggatgc tcccgccata gcggagaaga
    32941 ccggtgctat ggttgtcggg accgtcgaac tctgtgcggc tcttccagag ggggttaaaa
    33001 ctcacccgat gcagatcggc ggtgccttta ggttcccttt tggaaaagtc aggatgaccc
    33061 ctgcatggca cggttcgggg atcatggccg acggcgtgat ggtctacggt ggagttgccg
    33121 gaggctttct gatcgaggtt gatggaaaga gaatatacca tgccggagat acggggctga
    33181 cgatggagat gacactcctt gccgctgatt cagtcgacgt ggctcttctt ccgatcggag
    33241 gaaactacac catggacatc gaggatgccg tcagggcaac ggagatgatc cggccaagga
    33301 tcgtcattcc catgcatttt gactctttcc cagtgatcaa ggcctctccg gagagattct
    33361 cgtcccttgc gtcgggtcat gccgaggtcc gggtcctggg tgctggcgac agtctggata
    33421 tctgatatca ggaatatttg agcccgaata ccgggtggaa tattctttcg tcgtgcagaa
    33481 attccgcctg ctcatccatg gccttcctgg ttgcctctcc catattcacc gccagtacgg
    33541 aagtgagtat gtggacgagg aacagaggtc ctataatgct gctcccgaac atcgggctgg
    33601 cggcactgac aaagaagggc aggtccgagt gcctgcatat aggcgccgcc gggctgtccg
    33661 ttattgttgc gatccgggcc ccttgtttct ttgctatctc cactgcttct gttacctcta
    33721 tgacaaaact gggcagttca caaacgacga cgaggtcctt ttccccgata gatcttgcct
    33781 gttcctgcaa tgtcatcccg gatcttgtca tcagtatggc cctgataccc atggttctca
    33841 gccttatgaa aagggactcg gaaaccatgg aagatattcc ccatcccacg caatagacag
    33901 aatcagctcc ctgtatcatt tcacagaaag atgagacctg ctcgggcctg atctgggacc
    33961 aggtatcgtc aaggtttgcg tgctccagcc tgtgtattgt cgaagggaga tcctgcccgt
    34021 tgttaagaac cctggcgagc attgcggcag ggtttacctg tcccagcatc gccccctgca
    34081 gcgcgtcctt gagctgggag taaccctcga accccaacat tctggaaacc ctcacgagtt
    34141 gagccttgga tactcccagg tcatcggcta cgtcacctat agacctgaaa gctgcttctc
    34201 gcatgttctt gaggagatac tccacgacgc gacgtgcctt gtttggcatt ccctgcatct
    34261 tttccctgag aagttcctgc agttgggctg tttccaagga taacaccctt tcattgacaa
    34321 tattgttgga cccgataaat aaaccttaag tcgcaaaaaa ttgactcaag tctactttgg
    34381 cagggtcgtt aagtcaatgt ggtttaaacg agacgtcagg aaacaggagg caggaaagca
    34441 aaaggcgtaa acggtgaacg gtcaacggtg aacgggaagg gcctgaaggg caagggcgtg
    34501 agacgttaga cgttagacga ataaccaaat tcaactgcgt aaacggtgaa cggtcaacgg
    34561 gaagggccgg aagggcaagg gcgtgagacg tgagacgtga gacgtaagac gaaaaggcag
    34621 aggcaaagtg attgtcattc tgaggaccaa cgggaccaag cggaacgtcc gcctaccggc
    34681 gggaatctgg ttcaggcccc gtgaatcgtg agtcgtgagt cgggcttgtc attctgagga
    34741 acgacgggac gaagaatctg ggttaggtcc cgtgagtcgg ggatcgtgaa tcggatcaaa
    34801 caccagaacc agattcttct ccgccagtag gcggatcaga atgacaaatt cttgcgtccc
    34861 cggtcctcac aaagaaaggt tttctctgtg tggcttcacc atc
//
LOCUS       DAOTIQ010000010        33263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1466_length_33263_cov_7.294086, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000010 DAOTIQ010000000
VERSION     DAOTIQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_33263_cov_7.294086"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..551
                     /locus_tag="P5541_02640"
     CDS             171..551
                     /locus_tag="P5541_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70903.1"
                     /translation="MIVRMFWRGCRFRRKNYQDTPVDSFWVEATGEEAEKQIFPFLEP
                     EDKRESRLLAEGRENISALLGAAGHNYCFLVSRDISSIDPFPVFYPSHQLWNGYSPFE
                     IMPEKEISRLEDLKGIDLPLGKAQ"
     gene            870..1499
                     /locus_tag="P5541_02645"
     CDS             870..1499
                     /locus_tag="P5541_02645"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRV70904.1"
                     /translation="MPKKTLLLFLFDDVEVLDFAGPFEVFSVTNELNGESLLNIRTVS
                     VSGLAVSARNGLSVVPDLALDNAGSADILVVPGGYGVRALLDDEMVTGWIRGIAEKAE
                     LVLSVCTGSMLLARSGLLDGLKATTHHSVKEEFAAMYPDIPLSGERFVDNGRVITSAG
                     ISAGIDMSLYVVERLYGAEIAARTAAYMEYQRMCQNQDMIRDLLGDGAS"
     gene            1748..1903
                     /locus_tag="P5541_02650"
     CDS             1748..1903
                     /locus_tag="P5541_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70905.1"
                     /translation="MKLAVLIIAFFAVIAYRFAGESDATELKSRPLPLIDINLPVGFE
                     TATFALG"
     gene            1919..2494
                     /locus_tag="P5541_02655"
                     /pseudo
     CDS             1919..2494
                     /locus_tag="P5541_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            2670..3617
                     /locus_tag="P5541_02660"
     CDS             2670..3617
                     /locus_tag="P5541_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV70906.1"
                     /translation="MKVRVRTDFIGVLLSGVIWGTLSPAGKRLGMLGTDMLYVGFLRA
                     LVMTVGVGLFLRVCCPGKFRVRPGQLARIFLVAGPCIVGIYAGFFFALQYLSIPVTIV
                     IFFTHPLLTTAGSLFITRETPDRYQVAGALLTLAGVSVGVLMSGASLSGSLNPTGLAW
                     VLFASISMSLYSLFGRLSAQTGFVPQTTLFFYIQVSGLMWLAAVKSVVSGWEGSFVLS
                     GQQILLVSYVALIGSLVGYSLYFYSLRTVQASTASIVSSIEIVTAFALSSILLGQPPL
                     VNEIAGAALIVLAIVLVGRSTGIPEPERSIGIYPGKDFL"
     gene            complement(3593..4738)
                     /locus_tag="P5541_02665"
     CDS             complement(3593..4738)
                     /locus_tag="P5541_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV70907.1"
                     /translation="MTNQRGLITRFISGNFGIMAFGDIYFLLALYLQDVGVTDPGTLG
                     WILGIYFAASTISRPLTGMIIEKLPFRRVMLAASVLCFASGTGVALAGKSVALILFFR
                     AITGFSSSLFIVGLTTYQALTVPEEVRGSSFTLASAGTIAPLVAILPVAEWMLRNGHP
                     SLYIWSPLLPALLCLGVAYSLPPLEDFELRKGNWGTYSEIFKIPASRTLLASVILFAM
                     TDAVILSLAGLALEKGVLASSFISSQAMTGLLVRVFGFRIMDRIPRSRVAAFTFAVTA
                     GSSIAITFVSTNSGMILWGIVYGVAMGYGFPMHLSLIGDAVPERLRPKATSLVWFFMA
                     GSYFASPVMTGFLARWLNFAWAFRIICGVILLSVPFMYRRYRKSFPG"
     gene            5120..5980
                     /gene="proC"
                     /locus_tag="P5541_02670"
     CDS             5120..5980
                     /gene="proC"
                     /locus_tag="P5541_02670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRV70908.1"
                     /translation="MEKAGKICIIGAGTIGGSIARALAAGEGSCVIATRRRVDRIREL
                     EDQGVTVTSDNRWAASESDMVILAVKPNQVIHVLQDLDGCLEGKIVISFAAAIPTGLI
                     QKVAPGSLVIRAMTNTAVQIRQGYTVYACGEGVKGFALNKAEDIFRCLGAFQKVDEQY
                     LDVLTAMSGSGPAYIYTVIESMIYGALRVGLPRDLALQAAAHTAIGASQMLLVSGKHP
                     AELRDMVVTPGGVTIEGIYELEESRIRTAFMKAVCAASEKASSIASEARNSAEEDIRL
                     GRLEFDPVSA"
     gene            complement(5972..7252)
                     /locus_tag="P5541_02675"
     CDS             complement(5972..7252)
                     /locus_tag="P5541_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRV70909.1"
                     /translation="MPDLSPMLYSLKGKRILGVNPPVRDFAFFDLWAKPMGLLYLLGR
                     LRDLGNNVSLIDCVHEAGGKQKLFGRRVPGKFEIPKPAPYAHIPRRYWHFGLGREELV
                     SRLKGMPLPDLVLVTSSMTYWYPALFWAIAIIRATIPSAPVFLGGSYPVLCPEHARRS
                     GADFLQTETMPLPAALPAMDLYERINYGITMTSTGCFGRCSYCASRLLWPHHSKREFR
                     DVFAEVSFQAGLGATDIAFYDDALLEGKNLHFIPLCSSLKTMFPDLRFHTPNGLNVSE
                     IDMECAETMYRSGFSTIRLSLEGTDPENFRASRGKTTPEQYAGAVENLRSVGFAPDQI
                     ETYVLVGLPGQGFEEVAKTIGFVQSLGARAKIAQLSPIPGTDLFGEIEKRNPSVAEEP
                     LLQNNSIFCQYISKTLLPEELQKIKNLANPSRSG"
     gene            complement(7256..7882)
                     /locus_tag="P5541_02680"
     CDS             complement(7256..7882)
                     /locus_tag="P5541_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV70910.1"
                     /translation="MTFFLGALDDDPGILYTLEAMASSQGWNMKTTTNPSEALDWVKN
                     DSVDMLLLDLHMPVMSGLEVLREARKISSDAVLLVLTVEESPDVARDLHIAGADDFIS
                     KPVRLADFASRIALHAELGRYRREGRREQPRKGLSEEITRKVLDTVKKSNIPVDAKTV
                     AVKVGISYPTAHRYLEFLTSRGSLARSSLNVDGKPGRPRSLYQAISGE"
     gene            complement(7879..9267)
                     /locus_tag="P5541_02685"
     CDS             complement(7879..9267)
                     /locus_tag="P5541_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV70911.1"
                     /translation="MKRTLSPFSIKLLSSQLNWGSLLLVIWIVSQFYEKPIYHVFRLL
                     ELAVRFDDSGYLIAATTVLVVVNAARALLLYEGWFLIGNGISETSGKPMVDRVIPLVG
                     IPLCYQAVALFDFPSIPHFGMPAILGLASVLIVQYLTRSVTRWLNRAIALVLLLVSFQ
                     WLDVIPALTKYGFGWGELSMAIKNIAELPDKGLVLDIVGIIFFLCLFLGSLLTIELLV
                     SYERQLDQFRKFRKQERELSSLREQQLESRAVREIQHLVHDLKRPLTTIIGLADVMIE
                     KGGTRTSQHASVILKSSRSMDQMISEILDAGSGRRISLCDLVNYSLSQTSPMDWSKHI
                     EVDISPDCGKTSLHVNVIRFSRALANLLDNAAKATEGTDSPKILLSASATPLKATISV
                     EDNGPGFRRPPRPHRSGWGSTGLGLVFVEEVVKDHGGKISFGKSALGGAWVKIFLPMA
                     NGLEDVKKGGRK"
     gene            complement(9269..10318)
                     /gene="pgsW"
                     /locus_tag="P5541_02690"
     CDS             complement(9269..10318)
                     /gene="pgsW"
                     /locus_tag="P5541_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HRV70912.1"
                     /translation="MNHIKSPFLKTLWGSDDAVARKQISLLVAWAFFVSLLVWFPGGS
                     LSPVETRLVHTVVSAQGSIRQTRESLGTLAPSEIDPSGSGLIGVEWSPITTTMGSLES
                     KQLSVDPAWAPAFRRWYLDAGLSSGEKVTILSSGSFPGLALSAIAAAESLDLDITLIA
                     SLGSSTWGANTISMPLSAILRIIRENGFVRTTPSAYTLGGGGESGGGMAPEGITALLQ
                     SASRDNIQILVADSIEEMILKKGEIAFVPGTKLVVQIGGSQADIGTNPAVLDLPPGLL
                     FPYTEKNPGNGLIASALRLGIPVLHIIDIPQLAKEVGIPGDLSNPRKRSPARIIAAVA
                     TGAILLWKHQRWRLE"
     gene            complement(10315..10731)
                     /locus_tag="P5541_02695"
     CDS             complement(10315..10731)
                     /locus_tag="P5541_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HRV70913.1"
                     /translation="MNQDLAIIALGIALGMVFFQRTGFSAGGIISPGILALNMSSFYP
                     LVWTVVSSLFVFFLLEICVRFPGLYGRQRVATALLLAALLRLAAGDFLPFDPVWLGWV
                     IPGLVAADVQRQGILPTFSGLIAVSGTTFLIGGFLL"
     gene            complement(10728..11702)
                     /locus_tag="P5541_02700"
     CDS             complement(10728..11702)
                     /locus_tag="P5541_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV70914.1"
                     /translation="MKKEPPEYRLLVTGTRGKSTASRYLASAFGSIGLTCSTRITGTI
                     PTVIDHTGSRKIIRSCPGHISEMRWWLENIPEGTSAIVAENSAVSTEMQSLPAKWLKP
                     SLIIWTTLLPDHAEHWGPGVSGARRSLLEGVPEGSDVILGPQASRDKPLLSLLAAKRC
                     SIITIMENEKNFVEQYKAIAVKALDILGLPGSSASFGNIAEDRHAFRTVITPGGGLIA
                     WAFSSNDPDTAKNLFYSLGWKPEETVMLFNHRSDRPGRLISHFSFLNSLPWKAVWVTG
                     DRTFLPPGIRFKQIHTAQKMDSLASLSGRVFGCGNVRGLPFPEEVEKS"
     gene            complement(11783..13498)
                     /gene="ggt"
                     /locus_tag="P5541_02705"
     CDS             complement(11783..13498)
                     /gene="ggt"
                     /locus_tag="P5541_02705"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRV70915.1"
                     /translation="MRRIAVLFLAVSLLLSAGAALAAEVNEVMAKNGMVSSAHELASK
                     AGVEIMQEGGNAVDAAVATMLALNVVEPNASGLGGGGFMVIRNAKTGEVVFIDYRETA
                     PASATKDMYASQASKDESWSKVGGKAVGVPGQLMGIFAALEKYGTMSFAEVAAPAIRL
                     AEEGFEVHPMQTGIIADNFEKISKNNNVDDLAFFEDGLPIEAGKTLRQPGLAKAFKLI
                     SEHGVKAMTEGPIGESVVAAVNRTGGNMTMNDLKNYRVKISEPAHGTYRGYHIYSTPP
                     ASSGGTHIVQLLNIMENFPVASMKHNSPQYLHTMAEAMKLVFADRSKYMADTAFVNVP
                     LRGLTNKDYAKKLAEKISMDKVMVEVEADDPWPFNGDGKTSYLGGTDDQHISTSHFSV
                     VDKDGNIVASTNTVNYFFGSGVIATEFGLVLNNEMDDFSSDPASVNAPEPGKRPLSSM
                     SPSIVLDPAGQPFMTIGAAGAWRIITAVTQIVMNVVDFGMNMDEAIEQPRIFSYSLGG
                     KSGPLRIEDSMDPTTVDMLRFRGHNVEVQPRGGYYGTAQGILFVDGMMNGGADSRRLG
                     VPVGF"
     gene            complement(13575..14708)
                     /locus_tag="P5541_02710"
     CDS             complement(13575..14708)
                     /locus_tag="P5541_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRV70916.1"
                     /translation="MFEIRGSKSSAIFLLAAALTLSAWTGRNFLEMRKPDLVIPAEGF
                     SGHMLSEWFPGITGTAVDTPVFIQDSGIPGANVLIMGGTHPNEPAASMTAVLFLERAA
                     VTRGRLFVIPFANMSAFGHNSPQEASPQRIHFTLPDGSVRSFRYGSRATNPVHQWPNP
                     DIYIHEASGQQLSGSEMANLNRAHPGVEDGPLTQRLAKGLLELILKEKIDLAFDLHEA
                     SPEYPVVNAIVAHERSMELAAMTTMELEFEGISMRLEPSPKNLRGLTHREWGDFTETM
                     PILMETGNPSQGRLRGRTDEHLVLTGEDKFYAKASELGRLFIPYENDQPLDYRVARHT
                     ESIMVFFEMMTLLDTELVLAVENIPSFEEIMEKGMGEFLSPLN"
     gene            complement(14721..14867)
                     /locus_tag="P5541_02715"
     CDS             complement(14721..14867)
                     /locus_tag="P5541_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70917.1"
                     /translation="MMTLVYRGIALFILALVLRCMFREKDFWKQVTATMVIVPLVLRI
                     LLIK"
     gene            complement(14880..16166)
                     /locus_tag="P5541_02720"
     CDS             complement(14880..16166)
                     /locus_tag="P5541_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV70918.1"
                     /translation="MSWFWPEGFYTVAMIATFALGAFALRLPIAVAMGMAAVAGSLLG
                     GEGIPLRHLVEGMFGYIDTILIIATAMIFMKTIEAIGLLESVAAWMIRKFRTMPALLS
                     IGIMALIMVPGMITGSSTAAVLTTGALVAPVLIKLGVPVLQTAATIALGAIYGMIAPP
                     INLPAMIIGGGIDMPYVGFGIPLLICTVPLAVFTALWLVFPWLRKKEHDPTLEEELER
                     MERTRLTLRLFSPIIVLVLLLGGERLFPQVFPALGMPLSFLLASAVGLVSGAKWNITE
                     TLTKAVNDALPVMGILMGVGMFIQIMTLIGVRGFVVVSALALPAWLLYLGIATSMPLF
                     GAVSAFGSASVLGVPFLLALLGRNEILVGSALSLIAGLGDLMPPTALAGIFAAQVVGE
                     ENYFRVLRHCILPAVLTAAWGIAIILGAQYIAGFLY"
     gene            complement(16177..16731)
                     /locus_tag="P5541_02725"
     CDS             complement(16177..16731)
                     /locus_tag="P5541_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRV70919.1"
                     /translation="MKSVKRSTVLLVAILAILFLSTGAQAAEVALTSVGQSPDAMMVM
                     VVLKKLQIETDFDALMKPGDLKDHKVLVAVVGGSSKGLGAAGINSEQEKERSISLLEK
                     AKDAGVKILVMHVGGEGRRGTLSDLFIEAVVPFADRITIVKGGNHDGLFDTLRSEEVP
                     LVEVETIRAIDTPLNRDLSDWGVL"
     gene            16941..17513
                     /locus_tag="P5541_02730"
     CDS             16941..17513
                     /locus_tag="P5541_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRV70920.1"
                     /translation="MGKKCFALLLSVVFIVATGGAVLAAEFKSDMKIEGAGEEVTGKV
                     FVKGEKMRQEMSTPMGTSVTITDQDAGVMYVLMPEQKMYMEMPLDPDMMMLQTSSLEE
                     SLEGRAEIKKTGTETVAGYKCDIYEIKYNDPEMGESRAWVSQKLQYPVKVISKTSEGT
                     VTLTYFNIVEGGIDPEIFKVPGGYQKLSGY"
     gene            complement(17557..18735)
                     /locus_tag="P5541_02735"
     CDS             complement(17557..18735)
                     /locus_tag="P5541_02735"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HRV70921.1"
                     /translation="MLRRFAQEICDTTHEIIGHHVLVTDQNAVVIGSSDPTRIESVHA
                     PSITVMKRRIETYTDPVEAARMAGIRPGVTLPVSLGGEVVGSIAIAGDHREVARYGHL
                     VQKQAELFLREQVLHESAGARERAIRELVADIASFDPESDDHAVLVTRGKELGYDLEI
                     PRVCLVMEPCKSSPGWEGTRGSEMREASIRKEIEDILRSAFPEPSSFFSGIGYNRWAV
                     FSSAGTRQEENWFDLTAERFEKCTNNFAEMGLAFPAGAGSIALSIKELHYSYCEAWKA
                     LSIGMKKKEQGSIFRIGNYLVEDLLTTINVRKGKRFSEKILGDLKKMSDTEEMTKTFE
                     EWCNDPCCPGSVAKKLNIHRNTLNYRLEKIKRATGLDPRKFSEAFRLHIALALDMLHD
                     "
     gene            18902..20188
                     /locus_tag="P5541_02740"
     CDS             18902..20188
                     /locus_tag="P5541_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HRV70922.1"
                     /translation="MRLELHRVSVRNVLWGDTTKVEDGTLFVNKEEMLSVVRPDERIA
                     KASLELARPGESVRIIPVKDVIEPRCKLSGSGEVFPGFVGGMETVGEGATMVLDGAAV
                     VTCGPIVAFQEGLIDMSGPGSAYSPFSETCNVVFIAEPVEGLDQHSYEAALRESGLKL
                     AWYLADCCRSAKADLVKAYEMEGPVRESALYPHLPKIAYFCMSITQGLLHDTFLYGAD
                     MKHIVPTLIHPNEKMDGAMISGNCVSACDKNTTWHHLNDPIVEELYSRHGKDLVFLGV
                     IPTLEDTRLAGKERTSWFNANLARMLGADGVVISEEGYGNPDTDLCMNAAKCEKLGIR
                     TVIISDEAAGTDGKSQGLADATPEMTAFVSAGNVNEMVELPAMERVIGFPESIANLSG
                     GAEESLRKDGSMFVELQAIIGSTNELGFSRIGARWV"
     gene            20203..21516
                     /locus_tag="P5541_02745"
     CDS             20203..21516
                     /locus_tag="P5541_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21250..21252,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HRV70923.1"
                     /translation="MMTKRVVCYINQFFAGLGGEEQAHVPPSSKAGTMGPAMQIDNLL
                     GEDAEVVGTVICGDSYYGENMEKARVECLELVSAFGPDLLIAGPAFNAGRYGVACGDI
                     CKEVSERLEIPSITGMFPENPGVELYRSGTYIVPVADSARGMKNALEAIATLAGKMIR
                     GEKLGAAEDEGYISRAIRKNFFHEVSGARRAVDMLLKKLDGVSFNTEYEMPVFKRVTP
                     APPVMDMSKATIALVSSGGIVPRGNPDHIRVSSAESYGVYDISKLDDLTPEVFESIHG
                     GYDRVWANEDPDTVLPLDIMREMEKEGVFAKLYDHVFTTTGTGTAVSHAERFGREIGE
                     RLKKDGVDAVILTSTUGTCTRCGASMVREIENAAGIPVVQIATIVPIMLTVGANRIIP
                     GVAIPHPTGDPGRGPEGDRAVRRGLLERALRAIQTPVSEQTVFKS"
     gene            21645..22010
                     /locus_tag="P5541_02750"
     CDS             21645..22010
                     /locus_tag="P5541_02750"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HRV70924.1"
                     /translation="MLILTNNPRVAEKFPSSLFMEGSPHDVVSRAGDLLKGGYVLLSS
                     PLPPNGRLMKNPYRSIALAESGEKYRGGRDFVLVSEAAKRLAILEFLPKDGNRGEDLA
                     FMDLHLLEVAIREERNGQG"
     gene            22062..23546
                     /locus_tag="P5541_02755"
     CDS             22062..23546
                     /locus_tag="P5541_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV70925.1"
                     /translation="MAVSDRLDEIISVRLEKDQKNPAVWWKGSWIDGSGFMSLVLQTE
                     ESLRKSGFTRGSRIAVFMANSPLLWSIAVAAWRLGGTIAPLNARSGIKASVQITDHID
                     PVGAILGEGMESLAEALAAEGIPSVIAAPEGPLPVFEGRESVPDDDSIAVIFATSGTT
                     GSPKAVQVSHLNLIDNSFKVHRNIEGFESGRVLLNVLPNFHSLGFTICGVLPLCWGLP
                     MVIVSSFIPVRDTIEALRTAGVSIMIAVPTMLPFLLGAVSKEGISFPHLKYILTGGGK
                     LDPTLESRIRKEMGVICFEGYGLTECSPVVSCNPSEKTRKLGTVGPPLPGYEVQVRDP
                     EGSVLENGEDGVLWVKGPSVTSGYFRAPEMTAERFCDGWLDTGDIVNLDADGYITIMD
                     RATDLIIVGGFNVYPQEVETVLNSHPLVKMSAAIGVKSALSGEVVKAFVVLEDNTQVT
                     QQELVQFAKEHLSHYKVPRRIDIVQELPLSSVGKVLRRQLRDNE"
     gene            23784..24896
                     /locus_tag="P5541_02760"
     CDS             23784..24896
                     /locus_tag="P5541_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRV70926.1"
                     /translation="MRFIARVLLLIVFQVLAFSFVSFPVAAQPMVESAPEIRLTLLEI
                     RDEVLKGMEEVLRDHSGLLFTNTGERSFLSETAGLEMILHLYRGDRSSFDRQFRLVES
                     RFMGPLGLMAWKIAPENGSAELWNASVDDLRVAKALVLADKKWESADYRKAAMGLGAS
                     LLKYNTEGNLLVDGCSWKKPGLTGKSRVDQKYTTSTLAYADITAMRLLSGLDPEWVPV
                     LQRMSGVLIEGMAGRDCPRLLFESSTGNYEDTGQDDEIIVRMMAMLYLAESGFVNMAN
                     LDSTAFAAVSGRLLDNYGNENISVISLAALYLFHGGRKTEALQTLGRLDNFRNDVPEL
                     SGLLGYEESEGVYSAWAFDNLLALLAIETVLQNGSQ"
     gene            24918..25778
                     /locus_tag="P5541_02765"
     CDS             24918..25778
                     /locus_tag="P5541_02765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV70927.1"
                     /translation="MSERTLKRSIFIKLTAAFCGFMVLFTWQFMNFSVPFEDYVMSIV
                     TAGCLFVGLALGLRGSLIVALLVLFGYGTIKIGLVLWLQQPVLIGAPQLFWLLAVPAA
                     AFVSGSLHESVDGVIERLGTLNTEIEDLVAVDEMTGLDNAKRFAFRVVEEISRCKRYG
                     GEFSIMLVKIAFLDELEDVHGLRVQKLVVQKVAETLKGMKRLEDFIARIGRDEFSFVL
                     PNTPKDGTTVLKERVREKLPYLDISPAGEEFRRIRLTIRIGIATFPSEGEEYIPLLNV
                     ARRDYEYDHG"
     gene            25765..27339
                     /locus_tag="P5541_02770"
     CDS             25765..27339
                     /locus_tag="P5541_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HRV70928.1"
                     /translation="MTTARWSGWIISLISLFIFSVICGSCPAEARQPGRGRVLLVHDS
                     GDLLPSSTADPVLMMSILLSRFAEEVTVGGGGDYSREILSGFDGVVIVSTVEGISELK
                     LPETDVPVVWVGTGIPRNLPIQVNVGDEFFFNFDHVRYKDRLLFAGKQHQSVFLEAGD
                     GVEVLAQATDLQRSVPLAVHLPGMSLWIFSGIPFWEGGEMVFADILYDVFGAQWKKSS
                     IYVRLDGIDPFTDAGQVERVADWLRERMIPFAVSYSPANWEGGTGNLVTIERNRPLVD
                     LLKRLSEYDIPLVMRGFAGNHRRVPYENAAEFWDYENDAPLPEAGKILQERIADGISV
                     SARLGIRPSGFMAPGYKLPPELLDPVGDRFRIMAGRVQVSEVTSEASYVPPFPARVRN
                     MILLPENLGYVSRYRPQEDISLILSKSLDMTVVRGALGSFAYDPVLGVDMLDLIVSEM
                     RKQGYGIDFVFDIPEPVPILEGAEAIEDSSGFERSKRMGELAGYVLLISGVLAGLFLA
                     TIYIRRSLGRRRDLFS"
     gene            27336..28859
                     /locus_tag="P5541_02775"
     CDS             27336..28859
                     /locus_tag="P5541_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HRV70929.1"
                     /translation="MKALAAIFIISLSLLTFCGSSIAAPTALVLHDGLTETFAVSLVN
                     LLGHFREYEARTSLLDSTEPGEIVSAEAVFVLMAYDSPPPGPRVREALEKRTGLTVFA
                     GEHRWMGSLPSVLICDRVSYRGSSFWIEGYAVRKKEKPFGETVVSMSQSGQDVPFIEK
                     NGNRWMIHGIPMFEVPAWIMADVLHDILGISHAPKRTAMLRLEDINPSYSGERLKKLQ
                     DCIEYLYSEGVPFSMAVFPVFINARGEPRALLITDNEPLMKLLRRAEKMGGTVIMHGT
                     SHQYHQVSGEGSEFWDVETDGPIPDEKAYFHERMRYGLWLFKEAGLHPKLFEAPHYNM
                     PLSLQKELTGYFGTIAGSLMITDESYTISQDFPYIIYRSWAGLTVLPEQLGYIATDAE
                     SMSIVTILERLDILTAIMRDPFACFFYHPYVAGDLYLRKLVPQIRDRGYVFADVSQFG
                     GDPLVVVTSADHIWPLAYSKVPLSRYLPLIVGLIGAAILVIIYFRQTKKKKREMFRG"
     gene            28856..30121
                     /locus_tag="P5541_02780"
     CDS             28856..30121
                     /locus_tag="P5541_02780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV70930.1"
                     /translation="MIGPIDYVFIACLVVIWSVLLYHVVLSYAGYRYSLSAQKEKERL
                     DETPIGELPSVTVLVPAHNEEAVIEKTLLALAAMDYPTGKVEIICINDGSRDRTGKIA
                     RKISESSKGKIRVLDVPKERSGKGKSEALNFGLEHASGEIIAVYDADNTPERSALLYL
                     VRNLVEGKDALGAVIGKFRTRNQDRNLLTRFINLETIFFQWTTQAGRWNLYRLATIPG
                     TNFVVWTELLKSLKGWDPKALTEDTELSIRIYLAGKFIKMIPYAVTWEEEPERWPVWF
                     RQRTRWAMGNIYVLKKYFLSLLFRGKFRLYWDILYMLLIYFLFLTSVTTSLVIFLLGL
                     TGIGNIVLEGPFNILWFLAAVLFVTEMGISLTAEPGEDRATNLTIALVMYFTYSQLWL
                     IVMFNALIKSFLSLFRKGGHFWVKTERAG"
     gene            30121..32628
                     /locus_tag="P5541_02785"
     CDS             30121..32628
                     /locus_tag="P5541_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HRV70931.1"
                     /translation="MDLRLSGKICVVRVLACLFAGLFVTGVLSTPGECTVNSVRTGIQ
                     PGGARIVVDLDRASEYSVSRSGSELRITINTGIARQLSGNFPANRAASSYRALPSGNE
                     TLVTVVLKSPEVSVRHFKLVKPDRIVIDISPESKTAVSDTPTPKIAPAQAPAPAPAVP
                     ATGAQGLVPAPGSFGKGPQTFNSPLTQVDISVGSVFSSQSFFVDISPTWKILDGSYIH
                     LVLSHSQTTNPRISNVVVNVNNDPVYTIVLDRNNIWKGDIKIPVSPEYFGEGTNTITL
                     NSYMRSIEEQCQDIDNPGNWMRIHRESFVHVRYIPGSDLRLMEFTSPFFETNLNHKEN
                     SAIVIPDGFEPGEAEAAYEMLLSWARSSRFKDFRPKVIPISELTTEIASTFHIIYLGR
                     KEKFPKSVHDAFGTPASIKGSSYLSSFLNSQGKGRLLITAETSQGVSFGVRALMLPEL
                     REQMNVRNLSLALDTPLPQRKARSELDAELPFSQIFTGDVVFRGTYSHERFLSFRVPP
                     QWDLKGTPRIVLRYRHSPVLNERKSALTVDFEDVPRKSAPLGVTTSEEGRLDVPVPRD
                     IKGGDFLNFNLRAYLDIDTFDCGHNFTEAAWLVVDKSSYLHLPHTLKRISPLLEHLPH
                     TISDGNVNVYLGPGTDGNALTSLGNMLIYWQQNLLGDLFLSVKNLSAFSWDGAVGNSI
                     VLASVPDIRGKGIPISVGYDPAKKSIISGPKVPVVPEFGERAVLFQLLSQKDSSLALV
                     MGWARSVPGGDIFNDAIFRNRLRGDICLVAPDGRVVPFYLETPEPEPAGKISREWYME
                     IVDKFRGDRTTLGIFALGTSLVLFVLFVSLVRSMKKR"
     gene            32685..33257
                     /gene="tadA"
                     /locus_tag="P5541_02790"
     CDS             32685..33257
                     /gene="tadA"
                     /locus_tag="P5541_02790"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRV70932.1"
                     /translation="MIQSPLDVDGSDPMDRETLHHELFMRFAIEEALAAFEVGEVPVG
                     AVLVVDGEIAGRGHNMRESLTDPAAHAEMTALREAASGSGRWIIDGGTLYSTLEPCPM
                     CAAALVQSRVEAIVFGARDFRWGACGTLYDIPGDSRLNHRCKVTGGILAEECAKMLRE
                     FFRARRKGNRARCGEMAEWSKAGDSKSSRR"
BASE COUNT         7909 a         8173 c         9071 g         8110 t
ORIGIN      
        1 atacctcact ggatatcgaa accctgctca gactcatacc atgttggaca agagtctgtc
       61 ttgacgtatc tgaaattcta taataatctc tatagacgta tttattaaat taggtcaatg
      121 tattgatttt ttaggttttt ttccgggtta aaaaactgga ggagggttcc atgatcgttc
      181 gaatgttctg gcgaggctgc agatttcgga ggaaaaatta tcaggacact cctgttgatt
      241 ccttttgggt tgaggcaaca ggtgaagaag cagagaaaca aatattccct ttccttgaac
      301 ccgaagacaa aagagaatcc aggttgcttg ccgagggacg ggagaatatc tcagcacttc
      361 tcggggcagc gggccacaat tattgttttc ttgtctcacg ggacataagt tcaatagacc
      421 cctttccggt tttttacccc tcacaccagt tatggaacgg atattctccc tttgagatta
      481 tgcccgagaa ggaaataagc cgccttgaag atctcaaggg gatcgatctt ccgctcggca
      541 aggctcaata gggtaccggt tttttctgat aaaaagaata attcactctt taactaaaac
      601 gtgtttcctt ttccggggac tgtgcagatg aacctgtagt gttccgtgtt cgatggacgt
      661 aaacccacaa gccttgtgat cacccgccgg taaagcgtgc tttagaattg gagcgttttt
      721 tatcgaaaag accaatctgt ggttcctccc tcaattaatt aatcccatta aaaaagccaa
      781 tagcggttat cttcacgggt gttttcatgt ctgctaatat actgcagtga cttgaatgac
      841 tcttgaaaat ttgaagcgag ggaaatgtca tgccgaaaaa aactctttta ttgtttctgt
      901 tcgacgatgt cgaggtgctc gattttgccg ggcccttcga ggttttttcc gttaccaatg
      961 aactgaacgg ggaaagtctt ctgaacataa gaacagtatc cgtttccggt ctggcagtct
     1021 cagcaagaaa cggcctctcc gtggtccctg atcttgccct tgataatgcc ggttccgctg
     1081 atatcctggt tgttcccggc ggttatggag taagggcctt gctggatgat gaaatggtca
     1141 ctggatggat caggggtatt gccgaaaagg ccgaactggt cctgtccgtc tgtactggat
     1201 cgatgctgct cgcaaggtcc ggacttctgg atggcttgaa ggcaacaacg catcattcgg
     1261 tgaaggaaga gtttgcggcc atgtacccgg atataccttt gtcaggggag cgttttgtgg
     1321 ataatggcag ggtgattaca tcggccggaa tcagtgctgg tatcgatatg agtctttacg
     1381 tcgtagagag gctgtacggg gcggaaattg ccgccaggac tgcggcctac atggagtatc
     1441 aacggatgtg ccagaaccag gatatgattc gggacttgct aggggatggg gcatcctgag
     1501 aaggttgtcg tccgggaccc tgtctcctgc tcatgggtag tgaaagaatc catcctttcc
     1561 aggatggata ttgaagattc tggttgctat tacgaagatc tttccagacg ttgggggaac
     1621 tttacaatcc catacataag ggactgaaac tgcattcttc tcaccttctc aaaccctttc
     1681 attgttgacg tggggactaa aagagcttat tctaaataaa aacgttttaa aaaaaggggt
     1741 gagcggtttg aagctggcgg tgctgatcat cgcctttttt gccgttatag cctacaggtt
     1801 tgcgggagaa tctgatgcga cagagttgaa atctcgtcct cttcctctga tagacatcaa
     1861 tctgcccgtt ggatttgaga cagccacatt tgcccttggc tgattctggg gaccggattc
     1921 ccggttcggg agcgttaatg gcgtgataag gacaagggtt ggttattcag gggggagcac
     1981 cccggatcct acctaccaca agatagggga ccattccgaa agcatccaga tcgattttaa
     2041 cccagaggtt atatctttcg aagaactggc cgccattttc tgggaaagcc acaacccgtt
     2101 ctcaaagtct ttcagcatgc aatatgcctc gatcctgttc tttcacactc cggagcagga
     2161 gaagatagcg aaagacatgg ccggacgcct tcagacggaa agaggttcca gggtgagcac
     2221 tgaaatactt ccgttcagtg agtttttcct ggccgaggat taccaccaga agtatgtgct
     2281 caagggaaac aggtcggtgt acggggattt cagtgaaatc tatccagacg ataaggatgt
     2341 tgtcaattct actgctgcgg caaggctcaa cggttatctt ggcggcaatg ggtcttccga
     2401 aagatttctt gaggaaatat ctcttctcgg gctttccccg gaagcccggg acaggcttac
     2461 ggaggccttc tcttcaatgg ttccgcttaa gtgatggagt ttgtttctcg ttacatatcc
     2521 cccatcttat tggaaggcat ggattaaggg gcgtgtcttt aagggtggag ggcttttttc
     2581 gggatctaag gccagatttt ctgttctttt caggatgata caatatggaa caaattcatg
     2641 ccctgttccg gaagagaggt agttggcctt tgaaagttcg ggtaagaaca gattttatcg
     2701 gggtccttct atcgggagta atctggggaa cccttagccc tgcggggaag aggcttggga
     2761 tgctggggac ggatatgctt tacgtgggtt tcctcagggc ccttgttatg accgtcgggg
     2821 tagggctttt cctgagagtt tgttgcccgg gcaaattccg ggtcaggccc gggcaactcg
     2881 ccaggatatt cctggttgcc gggccgtgca ttgtcggtat atacgcgggg ttcttttttg
     2941 cgctacagta cctttctata ccagtcacca tagtaatctt ctttacccac cctcttttga
     3001 ccacagcagg ttccctcttt ataacaaggg agaccccgga caggtaccag gtagccggtg
     3061 cgctgctgac ccttgcaggt gtgtctgtgg gagttctcat gtccggggcc tccctgagtg
     3121 ggtctctaaa cccgacgggg cttgcgtggg ttctcttcgc ctcgataagt atgtcgcttt
     3181 attccctttt cggaagactt tcggcccaga cgggatttgt accccagacg accctgtttt
     3241 tctacatcca ggtatcgggt ctgatgtggc ttgccgctgt caagagcgtg gtttcaggct
     3301 gggaaggttc cttcgttctt tccggacagc agattttgct tgtatcctac gtggctctga
     3361 tagggagcct tgtaggatac agcctgtatt tctatagcct caggaccgtc caggcctcta
     3421 ccgcaagcat agtctcatcg atagagatag tcacagcctt tgctctttcc tcgatactgc
     3481 tggggcaacc gccactggtg aacgaaattg ccggtgccgc tctgatagtt ttggccatag
     3541 ttcttgtcgg gcgaagtacc gggatccctg aaccggaaag atccataggg atctatccag
     3601 ggaaggattt tctgtagcgc ctgtacatga agggaactga aagcaggatc actccgcaga
     3661 tgatcctgaa cgcccacgca aagttcagcc atcgagccag gaaaccggtc attacggggg
     3721 aagcgaagta actgccggcc atgaaaaacc aaacaagcga agtggctttt gggcggagcc
     3781 tttcaggtac cgcatcaccg atgagagaaa ggtgcatggg aaatccgtat cccatggcga
     3841 ccccgtaaac gatcccccag agtatcatcc ccgagttggt actgacgaaa gttatggcga
     3901 tcgaactccc ggccgttacc gcaaaggtaa atgccgccac cctgcttctg ggtattctgt
     3961 ccatgatacg gaaaccaaag accctgacca gaagccctgt catcgcctgt gatgatatga
     4021 aggatgaggc caggacacct ttttcgaggg caagacccgc cagtgaaagg attacagcgt
     4081 ctgtcatggc gaaaaggatc accgatgcga gaagagttct cgaggcggga attttaaata
     4141 tttcggagta tgttccccag tttcctttcc tgagttcaaa gtcctctaag ggtggaaggg
     4201 agtaggcgac tcccaggcac aggagcgcag gcaggagtgg tgaccagata tacagtgacg
     4261 gatgcccgtt tctgagcatc cattcagcaa caggaagaat agctacgaga ggggcgatcg
     4321 tgcctgcgct cgccagggta aaagaagaac cacggacctc ttctgggact gttagagcct
     4381 gataggttgt tagccctacg atgaaaaggc tcgaagagaa tccagtaatt gcccggaaga
     4441 agaggatcag cgcaactgat tttccagcca gtgctacacc cgtcccgctg gcaaagcaga
     4501 gaacagaggc tgcaagcatt acccggcgga aggggagttt ttcgattatc atgccggtaa
     4561 ggggacggga aattgtggag gctgcgaaat atatcccgag gatccagccc agtgtccccg
     4621 ggtcggtgac accaacgtcc tggaggtata gagctagcag gaaatatatg tccccgaaag
     4681 ccattatccc gaaatttccg ctaatgaatc ttgtgataag gcctctctgg ttggtcattc
     4741 acagggctcc tttgtatgca ttgacgtaag tttatcatga ttttgagaag ctctcccatt
     4801 tttctgcact tttgtctgca cttttatccc attgacttta actgtaaaaa aggtgtaagg
     4861 ttcctcaaac cgctaaaatg agtgggagac aaaaaggtat gaagaaatat gaagcatctt
     4921 taagtgattt tgttcctggc gcaaacccgt gccctgcaga ctcaaatcaa tgcagagccg
     4981 gttggtcgtg tcattttgcc aggtaacagt ttttgaaacc tgagagagca aagggccgcc
     5041 ggttaaaccg gcggcccttt tttgtgtgcg cccggcaggg cgcaccggtt tcttaatcca
     5101 gacggggggc tttattgtga tggagaaagc aggaaaaatc tgtattatcg gtgctggtac
     5161 cataggaggt tccatagcca gggccctggc agcgggtgaa ggaagttgtg ttattgcgac
     5221 aaggcgcagg gtggacagga tcagggaact tgaggatcag ggagttacag taaccagtga
     5281 taacagatgg gcggcctcgg agtccgatat ggttattctt gccgtaaagc cgaaccaggt
     5341 gattcacgtg ctccaggacc ttgacggctg tcttgaaggc aagatcgtta tatcttttgc
     5401 tgcggccatt ccaacagggt tgattcagaa agttgcgcca gggagccttg taataagagc
     5461 catgacaaat acagctgttc agatcaggca gggctacacg gtatacgcgt gtggagaggg
     5521 agtgaagggt tttgccctta acaaggccga ggatattttc cgttgtctgg gtgcctttca
     5581 gaaagtggat gaacagtatc tggatgtcct cacagcgatg tccggatccg ggcccgcata
     5641 tatttatact gtaattgagt caatgatata tggtgctctt cgcgttggcc tcccaagaga
     5701 ccttgccctt caggcagcgg cgcataccgc aataggcgct tcgcagatgc ttctggtctc
     5761 cggcaaacat cctgcagaac tcagggatat ggtggttact ccaggcggag tgactataga
     5821 gggtatctac gaactggaag aaagtcgaat aaggaccgca ttcatgaagg ctgtttgtgc
     5881 tgcctccgaa aaagcctcat ccatagcctc agaagcgaga aattcggcag aggaggatat
     5941 acgcctcgga aggcttgaat ttgaccctgt ctcagcctga gcgggaggga tttgcgagat
     6001 ttttgatctt ctggagttct tccggtaaga gggttttgga gatgtattga cagaagatcg
     6061 agttgttctg cagaaggggt tcttccgcga cagatgggtt tcttttttca atttctccga
     6121 acagatccgt tccgggtatg ggggataact gggctatctt tgcccttgct ccaagtgatt
     6181 ggacaaatcc gatagttttt gctacttctt cgaatccttg cccaggaagc ccgaccagga
     6241 cgtaggtctc tatctggtca ggcgcaaaac cgacactcct gaggttttcg acggccccgg
     6301 cgtattgttc cggggttgtt tttccccggg atgcacggaa attttcggga tcggtacctt
     6361 ccaggctgag ccggatcgtt gaaaaaccgg atctgtacat ggtttccgcg cattccatgt
     6421 ctatctcact cacgttgaga ccgttggggg tatggaagcg taagtcagga aacatggttt
     6481 taagggatga acagagggga ataaagtgta aattcttccc ttcgaggaga gcatcgtcat
     6541 agaaggctat atctgtcgcg cccagtccgg cctgaaaaga cacctcggcg aaaacatccc
     6601 tgaattctct tttggagtga tgcggccaca gcagtctgga tgcgcagtag gaacatctgc
     6661 caaagcagcc ggttgatgtc attgtaatac cgtagttgat cctttcgtaa aggtccatgg
     6721 cgggaagcgc cgctggcaga ggcattgttt cagtctgcag aaaatcagcc ccggaccttc
     6781 gggcatgttc aggacagaga acaggatagg aacctcctaa aaaaaccggg gccgaaggaa
     6841 tggttgcgcg aatgatggcg atggcccaga aaagagcagg ataccagtag gtcatggagg
     6901 aagtaacaag aacaaggtca ggaaggggca tgcccttaag tctggaaaca agttcttccc
     6961 tgccgagccc gaagtgccag tatcttctgg ggatatgggc gtagggggcc ggtttcggga
     7021 tctcgaattt tcctggtacc cttctcccga aaagtttttg tttccctccc gcttcgtgga
     7081 cgcaatcgat caggctgacg ttattgccca ggtccctgag cctgccgaga agatagagaa
     7141 gacccattgg ttttgcccaa aggtcgaaga acgcgaaatc ccggacggga gggttgacac
     7201 cgagtatcct tttccccttg agagaatata gcatgggaga cagatcaggc attgctcatt
     7261 ctcctgagat tgcctggtaa agggagcgtg gtctgcctgg tttcccgtcg acgttcagac
     7321 tgctccttgc cagacttccc cttgaagtta gaaattccag gtatcgatgt gcggttggat
     7381 atgatatccc gactttcacg gccacagtct ttgcgtcgac agggatattg ctttttttta
     7441 cagtatcgag aacctttctg gttatctctt ccgacagacc ttttcttggt tgttccctcc
     7501 gcccctcccg tcggtatctt ccaagttcgg catggagagc tattcttgag gcgaaatctg
     7561 ccaggcgcac tggttttgag ataaagtcgt ccgctcctgc aatgtgcagg tctcttgcaa
     7621 cgtcggggga ttcctccacg gtaagaacaa gaagaacagc gtcggaagat atctttcttg
     7681 cctcccggag gacttcaagg ccgctcatga ccggcatgtg aagatccaga agaagcatgt
     7741 cgacagaatc gttcttaacc cagtccaggg cttcagaggg attggtcgtg gttttcatgt
     7801 tccagccctg tgaggaagcc attgcttcaa gtgtgtaaag aatccctgga tcgtcgtcga
     7861 gtgctccaag aaaaaaagtc acttcctgcc ccctttttta acgtcctcta aaccattggc
     7921 catgggcagg aatattttta cccatgcccc cccgagagcg cttttgccga aagaaatctt
     7981 tccaccgtgg tctttcacaa cttcttccac aaaaacaagt cccaatcccg ttgatcccca
     8041 cccggacctg tgaggtcttg ggggacgcct gaaacctggt ccgttatcct ccaccgaaat
     8101 cgttgctttt aatggtgttg ctgaagcgct caggagaatt ttgggagaat ctgttccttc
     8161 ggtagccttc gcggcgttgt cgagaagatt tgccagggcc ctcgaaaatc gtatcacgtt
     8221 cacatgaaga ctggtcttgc cgcagtcagg ggagatatca acttcgatgt gtttgctcca
     8281 gtccatgggg cttgtctggc tcagggaata attgacaaga tcacaaagag atatcctgcg
     8341 gcccgaaccc gcgtcgagta tctccgagat catctggtcc atcgacctcg aggattttag
     8401 tattaccgaa gcatgctggc tggttctggt tccgcccttt tctatcatga cgtcggccag
     8461 acctattatc gttgtaagtg gtcgcttcag atcatgaacg agatgctgta tctccctgac
     8521 cgccctggac tcaagctgct gttcccgcag gctcgacagt tccctctcct gtttcctgaa
     8581 tttcctgaac tggtccagtt gcctttcgta actgaccagc agttcgatgg tcagcaacga
     8641 tccaaggaaa aggcaaagaa aaaagatgat ccccacgata tcgagaacca ggcccttgtc
     8701 gggtagttcc gcgatgttct tgattgccat ggaaagttct ccccatccaa aaccgtattt
     8761 cgtcagggcg ggaataacgt ccagccactg gaacgatacc agaagcagta ccagggctat
     8821 cgcccgattg agccacctgg ttacgctcct tgtcaggtac tggacaatga gtacacttgc
     8881 aaggccaaga atggccggca tgccgaagtg cgggatggaa ggaaaatcga agagggccac
     8941 agcctggtaa cagagcggta tccccaccag tgggataacc cggtctacca ttggcttgcc
     9001 ggatgtttcc gatataccgt tgcctatcag gaaccagccc tcgtacagca ggagtgcccg
     9061 ggccgcattg accacaacca ggaccgttgt ggctgcaata aggtagccgc tgtcgtcaaa
     9121 ccttacggcc agttcgagca gtctgaacac gtggtagatc ggtttctcgt aaaactggga
     9181 gacaatccag ataaccagga gcaatgatcc ccagttgagt tgtgaagaga gaagtttgat
     9241 tgaaaagggg ctcaatgtgc gtttcatttt attcgagtct ccatcgttgg tgcttccaca
     9301 acagaatggc gccggtcgct acagcagcaa ttatccgggc gggtgatctt ttccggggat
     9361 ttgacaagtc tccgggtatg cccacttctt ttgccaactg tggaatgtca atgatgtgca
     9421 agacaggaat gccaagcctc agtgcagaag cgatcaaacc gttcccgggg ttcttttccg
     9481 tgtagggaaa gagcaaacca ggaggaaggt ccagaactgc cgggttggtg ccgatgtcgg
     9541 cctggcttcc tcctatctgg acaacaagtt ttgttcccgg tacaaaagca atttccccct
     9601 ttttaaggat catttcctct atgctgtccg ccacaagtat ttgaatatta tcccgggaag
     9661 cggactggag gagagccgtg ataccttcgg gggccattcc gccgccgctc tcaccccctc
     9721 ccccgagagt gtaggcggag ggggtggttc tgacaaaccc gttttctctg attatacgaa
     9781 gtattgcgga aaggggcatc gaaatggtat tggcccccca ggttgaagag ccgagtgatg
     9841 ctatcagagt aatatcaaga tcgagagatt ccgctgccgc tattgcggac agggcaagac
     9901 ctgggaatga gcccgaactg agtatcgtaa ccttctcacc ggaactgagt cccgcatcca
     9961 ggtaccatct tcggaaggcg ggagcccatg ccggatcgac agacaactgt ttggattcaa
    10021 ggcttcccat ggtagttgtt atagggctcc actctacacc gataagacct gagcccgaag
    10081 gatctatctc tgagggagcc aaagtaccga gagattctct tgtctgccgg atcgatccct
    10141 gtgctgaaac tacagtatgg acaagcctgg tttcaacagg tgaaagagat cccccgggaa
    10201 accagaccaa cagtgagaca aaaaaagccc aggcaaccaa aagagaaatc tgttttctgg
    10261 caaccgcatc gtccgagccc caaagggtct taaggaaagg ggacttaatg tggttcacaa
    10321 cagaaatccc ccaatgagaa aggttgttcc ggaaacagca attaacccgg aaaatgtcgg
    10381 gagtattccc tgtctctgaa catcggcagc gaccagtccg ggaattaccc acccaagcca
    10441 caccgggtca aaaggcagga aatcccctgc ggccagcctg aggagtgccg caagcaaaag
    10501 agctgtcgcc actctttgtc tgccgtatag accgggaaag cggacgcaga tctcaagcag
    10561 aaagaaaaca aacagggaag aaacaacagt ccagaccaaa ggataaaaag aagacatatt
    10621 cagcgcaagg atcccaggtg aaatgatccc gcctgccgaa aacccggttc tctgaaaaaa
    10681 caccattcca agcgcgattc ccagggcgat gatagcaaga tcctgattca tgatttttcg
    10741 acctcttcgg gaaagggcaa tccccggaca tttccgcatc caaaaactct tccggagagc
    10801 gaggctaaag aatccatttt ctgagcggta tggatctgtt tgaacctgat gcccggaggc
    10861 agaaatgtcc ggtcacccgt cacccacacg gccttccatg ggaggctatt cagaaaactg
    10921 aaatgggata tcaatcttcc ggggcggtct gatcggtgat taaagagcat tactgtctct
    10981 tccggtttcc atccgagaga ataaaacaga ttcttcgccg tatccgggtc gttcgaagag
    11041 aacgcccacg cgatcaagcc tcccccgggc gtgataacgg tcctgaatgc atggcgatct
    11101 tcagcgatgt tcccgaagga agcacttgat ccaggcaggc ccaggatatc cagtgcttta
    11161 acggcaatgg ccttgtactg ttcgacgaag tttttttcgt tttccataat ggtaatgatc
    11221 gaacacctct ttgcagccaa aagagataaa aggggcttgt cccgggaggc ctggggaccc
    11281 aggataacat ctgatccttc agggactcct tcaagaagag atctcctcgc tccgctcaca
    11341 ccggggcccc agtgttctgc gtgatcagga agaagagttg tccagattat aagggaaggt
    11401 tttagccatt ttgccggcag ggactgcatt tctgttgaaa cagcgctgtt ctcggcgact
    11461 atggctgagg ttccctcagg gatattttca agccaccagc gcatttccga gatgtgaccc
    11521 gggcaagacc ttatgatctt cctggatccg gtgtggtcaa taacagtcgg gatggttcca
    11581 gtgatccggg ttgaacatgt caggcctatc gacccgaatg ccgaagcgag atatctggag
    11641 gccgtgcttt ttcctctggt tccggtaacg agcagcctgt attcgggtgg ttcctttttc
    11701 aaaatgcacc tcctgcaaaa aaggggcggc tgtaagccgc cccacaagat tactacaata
    11761 ccgggtcctt ttgggaagaa tcctaaaaac ctacaggcac gcccaggcgt cggctgtcag
    11821 ctccgccgtt catcattccg tcgacgaaca gtattccctg tgcggttccg tagtaaccgc
    11881 ccctcggctg gacctccaca ttgtgtcccc tgaacctgag catgtccact gtagtcgggt
    11941 ccatggagtc ctcgatgcgc aggggaccgc ttttgccgcc cagggagtag gagaagatcc
    12001 tgggctgttc tattgcctcg tccatattca ttccgaaatc cacgacgttc atgactatct
    12061 gggtcactgc ggttattatc ctccaggcac cggcagcacc gatggtcatg aatggctgtc
    12121 ctgccgggtc aagaacgata gaaggggaca tggaggagag gggacgtttg ccgggctcag
    12181 gtgcatttac gcttgcaggg tcgctcgaaa aatcatccat ctcgttgttg agcaccaggc
    12241 cgaactcggt ggctattaca ccggatccga agaagtaatt gacggtgttg gtggatgcca
    12301 ctatattgcc atccttgtcg accactgaga agtgactggt ggatatatgc tgatcgtctg
    12361 tgcctccaag ataggatgtt ttgccatctc cattaaaggg ccacggatca tcagcctcga
    12421 cttccaccat taccttgtcc atggaaatct tttcggccag ttttttagcg taatccttgt
    12481 ttgtcagacc tctcaggggt acgttaacga atgctgtatc ggccatatat ttgctcctgt
    12541 cggcgaacac cagtttcatc gcttcggcca ttgtgtgaag gtactgggga gaattgtgtt
    12601 tcatcgaagc cacagggaaa ttctccatga tgttgagtaa ttgaacgatg tgcgtccctc
    12661 cactcgaagc cgggggagtg gagtaaatat ggtagcccct gtatgttccg tgggctggtt
    12721 ctgaaatttt gaccctgtag tttttcaggt cgttcatggt catgttgccg cctgtcctgt
    12781 tgactgccgc cacaacagat tcaccgatag ggccttctgt catggctttg actccgtgtt
    12841 cggaaatcag tttgaaggcc ttcgccaggc ccggctgcct cagggtcttc cctgcttcta
    12901 tagggaggcc gtcctcaaag aaagccaggt cgtcgacatt gttgtttttg gaaattttct
    12961 caaagttgtc agctattatc cctgtctgca tagggtggac ctcgaagccc tcttcggcca
    13021 gcctgatagc aggagcggca acttcagcga aagacatagt cccgtatttt tccagggccg
    13081 cgaatattcc catcagttga cccggaactc ctacggcctt cccgccgacc ttggaccagc
    13141 tttcatcctt ggatgcctgt gaagcataca tgtcttttgt tgcagaagcg ggggccgttt
    13201 cgcggtagtc aataaagact acctcacccg ttttggcatt gcggataacc atgaaccctc
    13261 cgcctcccag gccggaagcg ttgggttcca ccacgttgag cgcaagcatg gtagccactg
    13321 ccgcatcgac ggcgtttccg ccctcctgca tgatctccac ccctgccttt gatgcaagtt
    13381 cgtgggccga agagaccatg ccgttctttg ccataacctc gtttacctca gctgccaggg
    13441 ccgccccggc cgagagcaga agtgacaccg cgagaaaaag aaccgctatc cttcgcatac
    13501 ctgttcctcc ttccataata tggaatacgt tcattctcta ttgcgaaccg ccccgctggg
    13561 agcggaggat cccactaatt gaggggagag agaaattctc ccattccctt ttccattatt
    13621 tcttcgaaag acgggatgtt ttccactgcc agaacaagtt cagtgtcaag aagagtcatc
    13681 atctcgaaga agaccattat ggactccgta tggcgggcaa ccctgtagtc caggggttgg
    13741 tcattctcgt aggggatgaa aagcctgccc agttctgacg ctttcgcata gaacttgtcc
    13801 tctccagtca atacgagatg ttcgtctgtt cttcctctta acctgccctg actcgggttt
    13861 cccgtctcca taaggatggg catggtctca gtaaagtcac cccattcacg gtgagtaagc
    13921 ccccgaaggt tttttggtga tggttcaagt ctcatggata taccttcaaa ctccagttcc
    13981 attgtcgtca tggcagcgag ttccatgctc ctctcgtgag cgacgatcgc gttgacaacg
    14041 ggatattccg gagaagcttc gtgaagatcg aaagcaagat cgatcttttc ttttaaaatt
    14101 agctccagaa ggccttttgc aagcctctgg gtcagaggtc catcctcgac accggggtgt
    14161 gcccggttga ggtttgccat ttcgcttccg gataactgtt ggcccgaagc ctcgtgaatg
    14221 tagatatcag ggttgggcca ctggtggaca gggttggtgg ctctcgatcc gtaacggaaa
    14281 gacctgacgc ttccatcggg aagagtgaag tgtatcctct ggggagacgc ttcctgtggc
    14341 gagttgtggc cgaaggcaga catgttggcg aaggggatga caaaaaggcg tccccgtgtc
    14401 accgctgccc tttccagaaa gagtactgcc gtcattgatg ccgcgggttc gttggggtgg
    14461 gtccccccca ttatcagaac gtttgctccc ggaataccgc tgtcctggat gaatacagga
    14521 gtgtctacgg ctgtacccgt tatcccgggg aaccattccg aaagcatatg gcccgagaac
    14581 ccttcggcgg ggatcaccag gtccggtttt ctcatttcca gaaaatttct tccggtccag
    14641 gctgacaggg taagggctgc cgcgaggagg aaaatggctg aagatttgct tccccttatc
    14701 tcgaacaaag gcgccacctc ctacttgatg agaaggatcc gcaggacgag aggaacaata
    14761 accatcgtcg ctgtgacctg cttccagaaa tccttctcac ggaacatgca tctcaggact
    14821 aaagcaagga tgaaaagggc gatccccctg taaacaagcg tcatcatgcc tgatgcctcc
    14881 tagtacagga accccgcaat gtactgtgcc ccgaggatta tcgctattcc ccaggcggcc
    14941 gtaagaacgg ccggcagaat acagtggcgg aggacccgga aatagttttc ctcgccgacg
    15001 acctgtgcgg cgaaaattcc tgcaagagcc gtgggaggca taaggtctcc gaggccggca
    15061 ataaggctta gggctgagcc gaccaggatc tcgtttctcc caaggagagc caaaagaaat
    15121 ggcactccca ggaccgatgc cgatccaaag gccgaaaccg ctccgaagag aggcatcgaa
    15181 gttgctatgc caagatagag cagccacgcc gggagagcca gggcagatac gaccacgaag
    15241 ccccttactc caatgagggt catgatctgt atgaacatcc ctacccccat caggataccc
    15301 atcaccggca gcgcatcgtt aacagcttta gtcagggtct ccgttatgtt ccattttgct
    15361 ccggatacaa gtccgaccgc agaggccagc aaaaaggaga gtggcatccc gagagcgggg
    15421 aagacctgag ggaagagacg ttcaccgcca agcaggagca caagtacgat aattggagaa
    15481 aaaagcctca gggtcagcct tgttctctcc attctttcga gttcttcttc gagggttgga
    15541 tcatgttcct ttttcctgag ccaggggaac actagccaga gagcagtgaa aacggccagc
    15601 ggtactgtgc atatcagaag gggtatacca aaccctacgt aaggcatatc gatcccccct
    15661 ccgattatca tggcgggaag atttattggc ggagcgatca tcccgtagat cgctccgagg
    15721 gctatggtcg cggcggtctg aaggactgga accccaagtt tgatcaaaac aggagcgaca
    15781 agagctcccg tagtgaggac cgcagcggta gaagaacctg ttatcattcc tggcaccatt
    15841 atcagggcca ttattccgat ggagagaagt gccggcatgg tgcggaattt cctgatcatc
    15901 caggccgcta ccgattccag aagtcctatg gcttcgatgg tcttcatgaa gatcatcgcg
    15961 gtggcgatta tgagaatggt atcaatgtaa ccgaacatgc cttcaacgag atgcctcaga
    16021 gggatgcctt ctcccccaag cagagagccc gcaaccgcag ccatacccat cgctacggct
    16081 atgggtagcc ttagggcaaa ggccccaagg gcgaaagtgg ctatcatggc gacggtgtaa
    16141 aatccttcag gccagaacca gctcatggcg ggccttctag agtactcccc agtccgacag
    16201 gtccctgttc agaggggtgt ctattgccct gatggtctcg acttctacca gcgggacctc
    16261 ctcagatctg agggtgtcga aaagaccgtc atggttgccc cctttcacga ttgtgatcct
    16321 gtccgcgaag ggcaccactg cctcaataaa cagatccgag agggtccctc ttcttccctc
    16381 accgcctaca tgcatcacca ggatcttcac accggcatct tttgccttct caaggagaga
    16441 gatggacctt tccttttcct gctcggagtt gattcccgcg gcccccagtc cttttgaact
    16501 tcctcctacc acggctacaa ggaccttgtg gtctttaagg tccccgggtt tcatcagggc
    16561 gtcgaaatcg gtctcgatct gcaatttctt gagtactacc ataaccatca ttgcgtcggg
    16621 gctctgcccg acagatgtca gagccacttc agcggcctgg gccccggtcg aaaggaagag
    16681 tatcgcaagg atcgcaacaa gaagaacggt ggatcttttc acggatttca agtcatacac
    16741 ctccattgtc aggccctgga tattgcaggc cgcaagttct tttaatacat ataaagggcc
    16801 gcagggcaaa aagtccataa tatttctcat atttatcatg cgcacggata aagaagcaca
    16861 ctgatgcgtc gatcggggcg atggtgtaaa ctcaacgcaa tgtttcagtt tttgtaaaga
    16921 aaatgagagg aggttcttcg atgggtaaga aatgttttgc cttgctgctg tcggtggttt
    16981 tcattgtggc aacgggagga gcagttcttg cggccgagtt caagagcgac atgaagatcg
    17041 aaggtgccgg ggaggaagtg actggcaagg tttttgtcaa aggtgagaag atgcgccagg
    17101 agatgagtac ccccatgggc acaagcgtca ccataaccga tcaggacgcg ggagtcatgt
    17161 atgttctcat gccggaacag aaaatgtaca tggagatgcc tctggatcca gacatgatga
    17221 tgctccagac ctcgagcctg gaagagtctc ttgaaggcag ggcggagatc aaaaagaccg
    17281 ggacagagac tgtggcgggc tacaagtgcg atatttacga gatcaaatac aatgatccag
    17341 agatggggga atccagggcc tgggtctcac agaaactgca gtaccctgtc aaggtcatat
    17401 cgaaaacatc cgagggaacg gttaccctta catacttcaa tattgtcgaa ggcggaattg
    17461 atccggaaat tttcaaagtt cccgggggtt accagaaact ttccggttac tgatggattt
    17521 ccgtcagccg gacttccatg gctccggctg aaacactcaa tcgtggagca tatcaagagc
    17581 aagggcgatg tgaaggcgga aagcctctga aaactttcgg gggtcgaggc ccgtcgccct
    17641 ttttatcttt tcaagtctgt agttaagggt gttgcggtgg atattcagtt ttttggctac
    17701 actccccggg cagcacgggt cattgcacca ttcctcgaag gttttcgtca tttcttcggt
    17761 atctgacatt tttttgagat cgccgaggat cttttctgaa aatctcttcc ccttccggac
    17821 gttgatggtg gtcagaagat cttctaccag gtaatttcct atccggaaaa ttgatccctg
    17881 ttctttcttt ttcattccta tggaaagggc cttccatgcc tcgcagtacg aataatgtag
    17941 ttctttgatc gacagtgcaa tgcttccagc tcccgcaggg aaggccagac ccatttcggc
    18001 gaagttgttt gtgcattttt cgaacctctc tgcagtaaga tcgaaccagt tttcttcttg
    18061 tctggtccct gcggatgaga aaacggccca tcggttgtac ccaatacctg aaaaaaaact
    18121 ggagggttcg ggaaaggccg acctcagaat atcctcgatt tctttgcgga tcgaagcctc
    18181 tctcatttca cttcctcttg tgccttccca cccgggagag gacttacagg gttccatgac
    18241 aaggcatacc ctcgggattt cgaggtcata accgagctct tttccccggg taaccagtac
    18301 agcgtgatca tcactttcag gatcaaagga agcgatgtcc gcgacaagct cacggatagc
    18361 tctctccctt gctccggcgg attcatgaag gacctgttct ctcaggaaaa gctctgcctg
    18421 cttctggacc aggtgcccat agcgggcgac ctccctatgg tctcctgcta tggcaattga
    18481 ccccacaact tcacccccaa gggagacggg aagggtcacc ccgggacgga tcccggccat
    18541 acgtgcggcc tcaacagggt cggtgtaagt ctctatgcgt cttttcatta cggtgatcga
    18601 gggtgcgtgc acgctttcga tgcgtgtggg gtcgctgctt ccgatgacca cggcattctg
    18661 gtcggtaacc agaacatggt ggccaattat ttcatgggtg gtatcgcata tttcctgagc
    18721 aaatcgcctc agcattttat ccgtctcctt cggtcacgtg gttgtggata ttttcccata
    18781 aagaggtgtt tgtgcaaatg cactaaacgc ccctatgcaa aagcggacaa cttccttaaa
    18841 gcgcccaata tgttgagtca aagggtgaac atataatacg cggagaaggg gggcgaagat
    18901 cttgcgtctg gaactgcata gggtatcagt caggaatgtc ttgtgggggg acaccacgaa
    18961 agttgaggat ggaacacttt tcgtcaacaa ggaggagatg ctttccgttg ttcgacctga
    19021 tgagagaatt gccaaagcca gccttgaact ggcaagacct ggcgagagtg taaggataat
    19081 cccggtaaag gatgtaatag agccgcgatg caaactcagt ggatccggag aggtttttcc
    19141 cggctttgta gggggaatgg agacggtagg agaaggggcc accatggttc ttgacggcgc
    19201 cgccgtggta acctgcggcc ccatagttgc cttccaggaa ggtctgatcg acatgtcggg
    19261 tccgggctcg gcctattctc ccttttcgga gacctgcaac gtggtcttca tcgccgaacc
    19321 ggtggaagga ctggaccagc actcctacga ggcagccctc agggaatccg gactgaaact
    19381 ggcctggtac cttgcggatt gctgccgctc tgcaaaagcg gacctggtca aggcctacga
    19441 gatggaagga ccggttaggg aatccgctct ttatccacac cttccgaaga tcgcctattt
    19501 ctgtatgtcc attacgcagg gacttcttca cgacaccttt ctttacggtg ctgacatgaa
    19561 gcacattgtt ccgaccctca ttcatcccaa cgagaaaatg gatggggcca tgatctccgg
    19621 caactgtgtt tccgcctgcg acaagaacac tacctggcac cacctgaatg atcccatagt
    19681 ggaggaactc tattcaaggc acggaaagga tcttgtattt ctcggggtta tacctaccct
    19741 ggaggacacc cggctcgccg ggaaggaaag gacgtcgtgg ttcaacgcca acctggcccg
    19801 gatgcttggg gccgacgggg ttgtaatctc tgaagagggt tacggtaacc ccgatacgga
    19861 tctgtgcatg aatgctgcca aatgcgagaa actgggcata aggaccgtga taatatccga
    19921 cgaagccgcc gggaccgatg gcaagagcca gggtcttgcc gacgccactc cagagatgac
    19981 ggcttttgtg tctgccggca atgttaacga aatggtggag cttccggcga tggaaagagt
    20041 aataggattc ccggagtcaa tagccaacct ttcgggtggt gcagaagaga gtcttcggaa
    20101 agatggctcc atgttcgttg agcttcaggc tattatcgga tcaacaaacg aactcgggtt
    20161 tagcaggatc ggggcccggt gggtctagaa cagggggcgg ttatgatgac aaagagagtc
    20221 gtctgttaca taaaccagtt tttcgcgggc ctcggggggg aggagcaggc tcacgttccg
    20281 ccctcatcga aggctggaac tatgggacct gcaatgcaga tagacaatct cctgggagaa
    20341 gatgccgaag tggtagggac agtaatatgt ggtgacagct attacggaga gaacatggaa
    20401 aaagccaggg tcgagtgcct ggaactggtc tcggccttcg gacccgacct tctgatcgca
    20461 ggaccggcat tcaacgcggg gcggtacggg gtagcatgcg gggatatatg caaggaggtc
    20521 tctgagaggc tggaaatacc ttcaataaca ggaatgttcc ccgaaaaccc cggagtggaa
    20581 ctctatagat cgggaactta tattgtgcct gtagcagata gcgccagggg tatgaaaaac
    20641 gcccttgaag cgatagcgac cctggccggt aaaatgatca ggggtgaaaa actgggagct
    20701 gccgaagacg aaggatatat ttccagagcc attaggaaga attttttcca tgaggtcagc
    20761 ggggccagaa gggctgtgga tatgctcctg aaaaaactgg atggcgtcag tttcaatacc
    20821 gagtacgaga tgccggtttt taaacgcgta actccggcac ccccggtcat ggatatgtcg
    20881 aaggcgacca ttgcccttgt cagctcgggg ggcatcgttc ccagggggaa ccccgatcac
    20941 ataagggttt catctgccga atcctatggt gtttacgaca tttcaaaact ggacgatctg
    21001 acgccggaag tttttgagtc catacatggg ggttatgaca gagtctgggc gaacgaagac
    21061 ccagatacgg ttctgcccct tgacattatg agagagatgg aaaaagaagg tgtattcgcc
    21121 aaactatacg atcatgtatt tactacgaca ggtactggca ccgccgtttc ccatgcggag
    21181 cgtttcggta gggagatcgg tgaacgcctg aaaaaagacg gtgttgacgc ggtcattttg
    21241 acctctacct gaggaacttg tacgcgatgc ggcgcatcga tggtaaggga gatcgagaac
    21301 gcggcgggaa tacctgttgt ccagatcgcg actattgttc ccataatgct cactgtaggg
    21361 gccaacagga taattccggg ggtggccatt cctcacccta caggtgatcc gggccgtgga
    21421 cctgaggggg acagggctgt aaggaggggg cttctggaga gagcgttaag agcgatccag
    21481 actccggtat ccgagcagac ggtcttcaaa tcctgataat ttccggtatt ccctggaaaa
    21541 gagggggagg ttttcctccc cctctttttt tatggcaatc tgttcccgat gggcgttata
    21601 cttgaatcat tccagagagc atcgaaaggg gagacagatc agaattgctg atccttacca
    21661 acaacccgcg cgtggcagaa aaattcccgt catccctgtt catggaaggc tccccgcacg
    21721 atgtggtctc cagggccgga gatctgctca agggtggcta cgttcttttg agttctcccc
    21781 ttcccccgaa cgggagactg atgaagaacc cctaccgttc aattgctctc gcggagagcg
    21841 gagagaagta ccgtggcgga cgtgacttcg ttcttgtttc cgaggcggcg aagcgtctgg
    21901 cgatcctgga atttttgccg aaagacggaa acagggggga agatctggcc ttcatggatc
    21961 ttcatctact tgaagtcgcc atcagggaag agcggaatgg ccaggggtaa ctttcgagga
    22021 aagacaaccc ggataatttt tttgaaagag aggggaagag aatggcagtg tccgacaggc
    22081 ttgatgaaat aatatccgtg agactggaaa aagatcagaa gaatcccgcg gtgtggtgga
    22141 aaggttcctg gatcgacggt tcgggtttca tgtcccttgt ccttcagacc gaagaatccc
    22201 tgaggaaaag tggttttacc cgtggctcca ggattgccgt atttatggcg aactcgcccc
    22261 tgctctggag tattgctgta gcagcctgga ggctcggcgg aactatagct cctcttaatg
    22321 ccagaagtgg tataaaggca agcgtccaga taacagacca catagatccg gtgggggcga
    22381 tcctcggtga agggatggaa tctctagccg aagctctggc ggcagagggg ataccttctg
    22441 ttatcgctgc tccggaaggt ccactccccg tatttgaagg cagggaatca gtgccggatg
    22501 atgactcgat agccgtaatt tttgctacct ctggtacgac gggttccccc aaggccgtgc
    22561 aggtaagcca cttgaatctt atcgataact ctttcaaggt ccacaggaac atagagggat
    22621 tcgagagcgg gagagtgctt ctcaacgtcc ttcccaattt tcactccctg ggatttacta
    22681 tatgcggagt tctgcctctc tgctgggggc tacccatggt tattgtatcc tcgttcattc
    22741 cggtccggga taccattgaa gcactgagga cagccggagt cagcataatg attgctgtac
    22801 ccacgatgct tccattcctt ctcggagcgg tgtccaaaga aggaatatct ttcccgcatc
    22861 ttaaatatat tttgacaggc ggaggaaaac tcgatccgac actggaaagc aggatccgca
    22921 aggaaatggg agtcatctgc ttcgaaggat acgggcttac ggaatgctct ccagtcgtat
    22981 cgtgcaatcc ctcggagaag acaaggaaac tgggtaccgt aggccccccc ttgccaggtt
    23041 acgaagtcca ggtaagggac cctgaagggt ctgttcttga aaacggagaa gatggagtcc
    23101 tgtgggtaaa gggtccttca gtaacatccg gctatttcag ggctcccgaa atgacagccg
    23161 agcgtttttg cgacgggtgg ctggacacgg gagatatcgt caatcttgat gccgatggtt
    23221 atatcaccat aatggacaga gccacggacc tgatcatagt cggaggattc aacgtatacc
    23281 cccaggaagt cgagacggta ctcaactctc atcccctggt gaagatgagc gcggcaatag
    23341 gtgtcaagtc cgccctgagc ggagaggtgg tcaaggcttt cgttgttctt gaagataaca
    23401 ctcaggtcac acagcaggaa cttgtccagt tcgctaagga acacctctcc cactacaagg
    23461 ttcccagaag aatagacatc gttcaggaac ttcctctgtc gagtgtggga aaggtcctga
    23521 gaaggcaact gagggacaat gagtaacagg taatcggcat tgcccggttg aaattatgga
    23581 aaacagaccg aagtccagtc cggagggttg gttatttttc gtaaaatggg gccaaagtct
    23641 catgcaatcg attgcatcaa gattgaactt taacacaaaa tgtagtgtat aattgccatg
    23701 gggtactata tgtgcatcgg gagttgtccc gacagaattc tggaagggag ggaccggtca
    23761 ggcagaggcc ggacacctgg aggatgaggt tcatcgcgag agtgttgctt ctgatcgtat
    23821 tccaggttct cgctttctcg ttcgtgtctt tcccggtggc tgcccagccg atggtggaat
    23881 ctgccccgga gatcaggctg acgttactcg agatcaggga tgaggtcctt aaaggaatgg
    23941 aggaggttct aagggaccat tcgggacttc ttttcaccaa caccggtgaa aggagttttc
    24001 tttccgagac agcaggtctt gaaatgatac ttcatcttta tcgtggggac aggtcctctt
    24061 ttgaccgcca gttccggctt gtagaatccc gttttatggg gcctctgggg cttatggcct
    24121 ggaagatcgc tcctgagaac ggttccgcag aactctggaa cgcgtcggtg gatgatctta
    24181 gagttgcaaa agctcttgtt ctcgcagaca aaaaatggga atcagccgat taccggaagg
    24241 cagccatggg cctgggagca tcccttctta aatacaacac cgaaggaaac cttcttgtcg
    24301 atggatgttc gtggaaaaaa cccggattga caggaaaaag ccgggttgac cagaaataca
    24361 ccaccagcac ccttgcctat gccgacataa ccgccatgag acttctttcc ggacttgatc
    24421 cagagtgggt tccggttctg caaagaatgt ccggggttct catagaagga atggctggca
    24481 gggactgccc caggttgctt ttcgagtctt ccaccggaaa ctacgaggat actggccagg
    24541 atgacgagat aatagtccgg atgatggcaa tgctctatct ggctgaatca gggtttgtca
    24601 atatggccaa cctcgatagc accgcttttg cggctgtttc cgggagattg cttgacaatt
    24661 acgggaatga gaacatttcg gttatctccc ttgcggctct ttatctgttc catggaggca
    24721 gaaagaccga ggctcttcag accctcggga gactggataa ttttcgcaat gacgttcctg
    24781 aactttcggg gcttctgggt tacgaagaga gcgaaggtgt ctactcggcc tgggcttttg
    24841 acaaccttct cgctctcctg gctatagaaa cggttttgca gaacggtagt caatgatcga
    24901 aaaggggtgt gataccggtg tcagaaagaa cccttaaaag aagcatcttt ataaaactta
    24961 ccgcggcttt ctgcggattc atggttcttt tcacctggca gttcatgaat ttttctgtgc
    25021 ccttcgaaga ttatgtcatg tccatagtaa ctgcgggatg ccttttcgtt ggtctggccc
    25081 tgggtctgcg aggaagtctg atcgtagcgc ttcttgtcct tttcggctat gggactatca
    25141 agatcggtct tgttctctgg cttcagcagc ctgttcttat aggagctcct cagcttttct
    25201 ggcttctggc cgttcctgcc gcggctttcg tcagtggatc tctccacgaa tcggtcgacg
    25261 gggtcataga aaggctggga actctcaaca ccgagataga ggacctggtt gcggtggatg
    25321 agatgactgg cctggacaat gccaagaggt tcgccttcag ggtcgtcgag gagatctcga
    25381 ggtgcaagag gtacggggga gagttttcta tcatgctggt gaagatagcc tttctcgacg
    25441 aactggaaga tgttcatggc ctcagggtcc agaaactggt ggtccagaag gtggccgaga
    25501 ctctcaaggg aatgaaaaga ctggaagact tcatcgcgag gattggcagg gacgagtttt
    25561 catttgtcct tccaaacacc cccaaagatg gcactacggt gctcaaggaa agagtacggg
    25621 aaaaacttcc ctatctggat atttcccccg cgggtgaaga gttcagaagg atcaggctga
    25681 ccataagaat aggtatagca acgttccctt ctgaaggaga ggagtatatc cccctgctga
    25741 acgtggcgag gagggattac gagtatgacc acggctaggt ggtcggggtg gataatttct
    25801 ctgatatctc tttttatttt ctccgttatc tgcggatctt gcccggctga agccagacaa
    25861 ccaggaagag ggcgggtttt gcttgtccat gattcgggag acctgctgcc ctccagtacc
    25921 gcggatcctg tccttatgat gagcattctc ctgtcccgtt ttgccgagga agtaacagtc
    25981 ggcggcggag gggattattc ccgggaaata ttgtcaggtt tcgacggggt tgtaatagta
    26041 tcaactgtag aggggatctc ggaactcaag ttgcccgaga ccgatgtccc ggttgtctgg
    26101 gtcggtacag gaattcccag gaatcttccg atccaggtca atgtcgggga tgagtttttt
    26161 ttcaacttcg accatgtcag gtacaaggac aggctcctct ttgcgggcaa gcagcatcag
    26221 tcggtttttc tggaagcggg ggatggtgtg gaagtgcttg cgcaggctac ggatctgcag
    26281 agaagtgtcc cccttgcggt tcatcttccg ggtatgtctc tctggatctt ctccggtatc
    26341 cccttctggg aaggagggga aatggtgttc gctgacatcc tctatgatgt cttcggagcc
    26401 caatggaaga aaagttcgat ttatgtgagg cttgacggaa ttgacccctt cactgatgcc
    26461 ggacaggtgg aaagagtagc ggactggctc agagaaagga tgataccctt tgccgtttcc
    26521 tattctccgg ccaactggga gggtggaacg ggcaatcttg tcaccattga aaggaaccgc
    26581 cctcttgtgg atcttctgaa gcgcctttcg gaatatgaca ttccgctggt gatgagaggc
    26641 tttgcaggaa accaccgccg ggttccatac gagaacgctg ctgaattctg ggattacgag
    26701 aacgatgctc ccctgccaga ggcagggaag atccttcagg aacggattgc agacggtatt
    26761 tcggtttccg cccgactggg gataaggcct tcggggttca tggcgccggg atataaactc
    26821 ccgcccgaac tcctcgatcc ggtcggcgac agattcagga tcatggcagg aagggtccag
    26881 gtcagcgaag tgacttcaga ggcatcctac gtcccgcctt ttccggcccg ggtaaggaac
    26941 atgatcttgc tgccggagaa cctgggctat gtatcacgat acagacccca ggaggatatt
    27001 tcgctgatac tttcgaaaag cctcgacatg accgtggtga ggggggcact gggatctttt
    27061 gcctatgatc ccgttcttgg ggtggatatg ctggatctca tcgtgtcgga gatgcgaaaa
    27121 cagggttacg ggatagactt tgtttttgat attccggaac cggtccccat actcgaaggg
    27181 gcggaagcca tcgaagactc aagcgggttt gaaagatcga aaagaatggg agaactggcc
    27241 ggatacgtgc ttctgatttc cggagtgctg gctggcctct tcctggcgac aatatacata
    27301 aggagatcac tgggacgaag gagggatctg ttcagttgaa ggcccttgct gccattttca
    27361 ttatttcttt atctctcctg accttctgcg gttcctctat tgccgctccg acggcactgg
    27421 tgttgcatga tggcctgaca gagacctttg ccgtatccct tgtcaatctg ctgggacatt
    27481 tcagggaata cgaagcaaga acttcgctcc tggacagcac agagcctggc gagattgttt
    27541 ctgccgaggc tgtgtttgtc cttatggcct atgattcacc ccctcccgga ccgcgggtaa
    27601 gggaagcact tgaaaaaagg acgggcctga cggtttttgc cggggagcat cgatggatgg
    27661 gatctcttcc ctctgttttg atatgcgaca gggtcagtta caggggcagt tccttctgga
    27721 tagaagggta tgcagtcaga aaaaaggaaa aaccctttgg cgagactgtg gtttcaatgt
    27781 cccagtctgg tcaggacgtt cctttcattg agaaaaacgg taatcggtgg atgatacatg
    27841 gtatcccgat gtttgaggtc ccggcctgga taatggccga tgttctccat gatatcctgg
    27901 gaatatccca tgctccgaaa aggacggcaa tgctgcggct tgaagatata aacccgtcat
    27961 attctggcga aagactgaaa aaactccagg actgtataga gtacctctac agcgagggtg
    28021 ttcccttctc aatggcggtt ttccctgtct tcataaacgc aagaggggaa ccccgagctc
    28081 ttttgatcac cgacaacgaa ccattgatga aacttctgcg aagggcggag aaaatgggcg
    28141 gaacagtgat catgcacgga acctcccatc agtaccacca ggtctccggg gaagggtccg
    28201 agttctggga cgttgagact gacggtccca taccggacga aaaggcctat tttcacgaga
    28261 ggatgaggta tgggttatgg ctctttaaag aggccgggct tcatcccaaa cttttcgagg
    28321 ctccccacta caacatgccc ctgagcctcc agaaggaact gacaggttac ttcgggacca
    28381 ttgccggctc ccttatgatc accgatgaaa gctacacgat ttctcaggat ttcccctata
    28441 ttatctacag atcctgggcg ggtctcacgg ttcttccgga gcaattgggg tacatcgcta
    28501 cagacgcgga atccatgtcg atcgtcacca ttctggagcg gcttgatatc cttactgcga
    28561 tcatgcggga tccattcgcc tgtttctttt accatcccta tgttgccgga gacctgtatc
    28621 tgagaaaact agttcctcag ataagggaca ggggttatgt tttcgcagac gtttcgcagt
    28681 tcgggggcga tccccttgtt gtagttacaa gtgcagatca catctggcct cttgcttact
    28741 cgaaggtccc attatcgaga tatctgcctc tgatagtcgg gcttattggt gccgctattc
    28801 tggtaataat ctatttcagg cagacaaaaa agaaaaaaag ggagatgttc cggggatgat
    28861 cgggcccatc gattatgttt tcatagcatg tctggtcgtt atatggagcg tgcttcttta
    28921 ccacgtggtc ctttcctatg ccggataccg gtactccctt tcggcccaga aagagaaaga
    28981 acgtcttgac gagaccccta tcggagaact tccttcggtg actgttcttg ttccggccca
    29041 taatgaggaa gcggtaatag aaaaaaccct gcttgctctc gcggctatgg attatccgac
    29101 gggcaaggtg gaaataatct gcattaacga cggttccagg gacaggacag gaaagatagc
    29161 aagaaaaata tccgaatcat caaagggaaa gatccgggta ctcgatgttc cgaaagaaag
    29221 atcgggaaag ggcaaatcag aggccctcaa cttcgggctc gaacacgcgt cgggggagat
    29281 catagccgta tatgatgccg ataacacccc cgaaagaagc gcccttcttt acctcgttcg
    29341 aaaccttgtc gagggaaagg atgctcttgg ggctgtgata gggaaattcc ggacccgcaa
    29401 ccaggacagg aatcttctga ccaggtttat aaatcttgag acgatctttt tccagtggac
    29461 gactcaggct ggaaggtgga acctctaccg tctggcgaca attccgggga ccaattttgt
    29521 cgtctggacc gaactgctga agtcgctgaa gggctgggat cccaaggcgc tgacggagga
    29581 caccgaactt tctatcagga tctaccttgc aggaaaattc atcaagatga ttccctatgc
    29641 ggttacctgg gaggaggagc cagaaaggtg gccggtatgg tttagacaga ggacaaggtg
    29701 ggccatgggg aacatctacg ttctgaaaaa gtactttctg tcgcttttgt tcaggggtaa
    29761 attcaggctt tactgggaca tactgtacat gcttcttatc tattttcttt tcctgaccag
    29821 tgtgacaaca tctcttgtaa tattcctgct tggacttaca ggcataggaa acatagtgct
    29881 tgaaggcccc ttcaacattc tctggtttct ggccgcggtt cttttcgtta cggagatggg
    29941 gatatcccta accgcggaac ccggcgagga cagggctaca aatctgacga ttgcactggt
    30001 aatgtacttc acgtacagcc agctctggct gatcgtgatg ttcaacgcgc tgataaagag
    30061 tttcttgtcg ctttttcgaa agggaggaca cttctgggta aagacggaga gggcggggtg
    30121 atggatttga ggctttctgg aaagatctgt gttgtaaggg ttcttgcctg tttatttgcc
    30181 ggattgtttg ttaccggagt tctgtctact cctggtgaat gtactgtaaa ctctgtcagg
    30241 accgggattc agcccggcgg agccaggatc gtggtcgatc ttgacagggc ttcggaatat
    30301 tcggtctcaa ggagcggtag tgagctccgg atcaccatca atacggggat agcgaggcaa
    30361 ttgtcgggca acttcccggc aaaccgtgca gcatcttcct acagggcctt accttcaggg
    30421 aacgaaaccc ttgttacggt agtgctcaag tcacccgagg tttcggtcag gcattttaaa
    30481 ctagtgaagc cagacaggat agttatcgac atatctccgg aaagtaaaac ggctgtgtcg
    30541 gatactccca ccccaaaaat agccccagcg caggcccccg cccccgcgcc ggctgtgcca
    30601 gctaccggag cgcaaggact tgtgccggcc cctggttctt tcgggaaagg accacagacc
    30661 ttcaactctc cccttactca ggtcgacata tctgtcggga gcgtcttttc cagccagtcc
    30721 tttttcgttg atatatcccc gacatggaag atccttgatg gaagttacat ccatctcgtg
    30781 ctcagccact cccagaccac caatccccga atatccaacg ttgtggtcaa cgtcaacaac
    30841 gatccggttt atacaattgt tctggaccga aacaatatct ggaagggaga cataaagatc
    30901 cccgtttccc ctgagtattt cggggagggg accaacacaa ttaccctcaa cagctacatg
    30961 cgatctatcg aggaacagtg tcaggacata gacaatccgg ggaactggat gaggatccac
    31021 agggaaagct ttgtacatgt caggtatatc cccggaagtg atctcaggct gatggagttt
    31081 acgtcgcctt tttttgaaac aaacctcaac cacaaggaaa acagcgcaat agtgatccct
    31141 gacggttttg aacctggcga agcggaggcg gcttacgaga tgctcctgtc ctgggcaagg
    31201 agttccagat tcaaggattt ccggccaaag gtcataccga taagcgaact gacaacggag
    31261 attgcttcca ccttccacat aatctacctt ggaagaaagg aaaaattccc gaaatcggtt
    31321 catgacgcat ttgggacacc agcgtctatc aaggggtcca gttatctatc ctctttcctt
    31381 aacagccagg gaaagggcag gctgctgata actgccgaga cctctcaggg tgtcagtttc
    31441 ggggtcaggg cccttatgct tccagaactc agggagcaga tgaacgtcag gaatctttcg
    31501 cttgctcttg atacgcccct gccccaaaga aaggccagga gtgaacttga cgcggaattg
    31561 cctttttccc agatattcac tggagatgtt gtcttcaggg ggacctacag tcacgagaga
    31621 ttcctctctt tcagggttcc gcctcagtgg gacctgaaag ggacccctcg gatagtactt
    31681 agatacaggc actccccggt tctgaacgaa cgcaaatccg ccctgaccgt tgatttcgag
    31741 gatgtaccta ggaagtcggc tcctcttggt gttaccacat ccgaggaagg tcgtctcgat
    31801 gttccggttc cgagggacat caagggtggg gactttctca atttcaatct cagggcatac
    31861 ctggacatag atacctttga ctgcggccat aatttcacag aagcggcctg gcttgtggtg
    31921 gacaaatcat catatctcca ccttccccac acccttaaaa ggatctcacc ccttcttgag
    31981 catcttcccc ataccatatc cgacgggaat gtgaacgtat atcttggccc aggtactgac
    32041 ggcaatgccc tgacatccct tggcaacatg ctcatatact ggcagcagaa cctccttggg
    32101 gacctgtttc tctcggtgaa gaacctgagt gccttctcat gggatggagc cgtgggcaat
    32161 tcgatagttc tggcctctgt accggatatc aggggaaagg gcatccccat atcggtcggg
    32221 tatgaccctg cgaagaaaag tataatttcc ggccccaagg taccggttgt tcctgaattc
    32281 ggggaaaggg cggtcctttt ccagcttctc tcccagaagg attccagcct tgccctggtg
    32341 atgggctggg cgagatccgt tccaggtgga gacatcttca acgacgcgat cttcaggaac
    32401 agactccgtg gcgatatatg tcttgttgct cccgatggca gggtcgttcc cttctacctg
    32461 gagactccgg aacccgaacc ggcagggaaa atttccaggg agtggtacat ggagatcgtt
    32521 gacaagttca ggggcgacag gacaacactc ggcatattcg cacttggaac atccctggtg
    32581 ctctttgtcc tcttcgtgtc tcttgtcagg agcatgaaaa aacgctgacc cggttcgaag
    32641 aaaaactttt ttcagggatc tcccctttcg ggaggtccct ttttttgata caatccccgc
    32701 tggatgttga cgggagtgat cccatggata gagagaccct tcaccacgaa ctgttcatgc
    32761 gctttgccat cgaagaagct cttgcagcat tcgaggttgg ggaggttccc gtcggagccg
    32821 tcctggtggt tgatggagaa atcgcgggac ggggacacaa catgagggaa agccttacgg
    32881 atccggccgc tcacgcagaa atgactgctc tcagggaagc cgcttcaggg tccggaagat
    32941 ggatcatcga tggcggcact ctctattcga cccttgagcc gtgtccaatg tgtgctgcgg
    33001 ccctggtgca gtcaagggta gaagccatag ttttcggagc cagggacttc aggtggggag
    33061 cctgcggcac cctgtacgat attccagggg attcaaggtt gaaccatagg tgcaaggtta
    33121 ccggtggcat actcgccgaa gaatgtgcta aaatgctccg ggagttcttc cgggccaggc
    33181 gaaaaggtaa ccgggcaagg tgtggagaga tggccgagtg gtcgaaggcg ggcgactcga
    33241 aatcgtctag gcggtgatga gcc
//
LOCUS       DAOTIQ010000011        32483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1533_length_32483_cov_6.971198, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000011 DAOTIQ010000000
VERSION     DAOTIQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 32483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32483
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1533_length_32483_cov_6.971198"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="P5541_02795"
     CDS             <1..583
                     /locus_tag="P5541_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV70933.1"
                     /translation="PLGILGMIIGMSMRRMGIVTEPASALSHFIATGFHPLVGGVLWG
                     GILITVIGTAAGLTLGVAVNIVSDILAPRFPGLCGGNSSATATRCAVLALILAAGLAG
                     IAGQGSMILKWSYLSMGLRGSGIFFPLVASVLFPGRLSPGMAAISGGAGLLATIMWPL
                     SGLPLEPLFAGLSVSAAVVFLGIRYGRREHPLP"
     gene            complement(596..1096)
                     /locus_tag="P5541_02800"
     CDS             complement(596..1096)
                     /locus_tag="P5541_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRV70934.1"
                     /translation="MAGKTVAWDLVDIEIPEGCNVILGQSHFIKTVEDLFEVLATSST
                     VLEFGLAFCEASGPCLVRSEGNSSDLVEAARDNALALGAGHSFVILMRNGFPISVLNG
                     IKQVQEVCRIFAATANPLQVLVFGSEQGRGIAGVIDGNSPTGVESEADVKDRKDLLRN
                     IIGYKR"
     gene            complement(1129..1962)
                     /locus_tag="P5541_02805"
     CDS             complement(1129..1962)
                     /locus_tag="P5541_02805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV70935.1"
                     /translation="MKKLLVADIDGTLAHDDHVPEVVADACHALVARGWDMVLATGRI
                     LGSAKKHIIRTGASSITIVYDGARVMDAATGREIWGQKLDPRVVKDVLEMLWDIPWGI
                     QVFRDENVLCKKGDDLAAEYFSKIGVPVDEGLLFPEPIEGVYRVILYGDPGEVESAGQ
                     RVGASRLAGECRAVMAGNGFLDILPSGVSKGAALQKMISLMQESERPSIVAAAGDHLN
                     DLEMLRFADIAITMDDARASLREVAHIVLPPASAGGFAHILEPLEEILSESVSEGSGR
                     K"
     gene            2117..3274
                     /locus_tag="P5541_02810"
     CDS             2117..3274
                     /locus_tag="P5541_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV70936.1"
                     /translation="MRKILVLALLAVFAVSGFAAPALSADVIKVGYLAALTGDWAAYG
                     QTELNAAKLAVAEINAKGGVLGKQIELFPYDFRTRPEDAVNAFRRMAENDKVVAVVGA
                     NGSGINIATAPLANKYGIPQIGTVSTNLLVTVNEKGELNPFMFRTCFTDPYQGQAIAV
                     FTAKELKKKTAAVLYDVGSDYSQGLREFFIQYFEEYGGKVVADQGFRAGTDVDFRAQL
                     TTIRDSKAEVLVLPNMGKEMALIMKQARELGMDDIVFVGGDGYGEFMWEIAGDAMENS
                     YWINHVAPEDPAMADFFANYKKTYNDECKEFVNGILAYDSIYWLADAIERAGKVDSTA
                     IRDALRNTVNVQLHHAVMSIDPETHNPKNKTAVVLVAKDGRAQFFTKVTPE"
     gene            3370..4251
                     /locus_tag="P5541_02815"
     CDS             3370..4251
                     /locus_tag="P5541_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV70937.1"
                     /translation="METLLQQVINGLSLGSVYALIAVGYSLVYSILLFSNFAHGGFLV
                     IGGYICYYTLAAVGHNIWIASLLAMVGAGVAAVMVERLAYKPIRERTNVTLYLLIASM
                     GMSIVIENIFVVTVGGRFRALPPVIPMNPVSVFGIATTSAFDILSLVMAVLFLTGLQI
                     FLSRTKWGLAIRAASYDLRTAGLMGVNVNKLISIVFFVAGLLAAVGGIFLSVRYTLYP
                     QLGLITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDIVVFGLLILT
                     LLWRPTGLFGKHVSEKV"
     gene            4264..5145
                     /locus_tag="P5541_02820"
     CDS             4264..5145
                     /locus_tag="P5541_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV70938.1"
                     /translation="MSGYTEGIIILICINSIAAMGISLLTGFTGIFSLGHAGYMAIGA
                     YTAAILSVRHDVPWIVAVVAGGMLAMLIAWLIGIPTLRLMGDYYAIASIGLGEAIRLV
                     LENWQSFTRGARGFPGIDNFTTLPVAAGFFIGMLVLMWFLISGNYGRAFKACRDDYIA
                     ASLLGFKTAHYRVLSLAISGFYCGIAGALMAGFLTFLQPIMFDLNKSTELTAVVVFGG
                     LGSLSGSIIGTTVIGIVTEVFRPISQYRMLIYGAIMVIIMVMRPEGIMGQHELGPRLL
                     RSLFSRKPAGKENATNG"
     gene            5138..>5511
                     /locus_tag="P5541_02825"
     CDS             5138..>5511
                     /locus_tag="P5541_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV70939.1"
                     /translation="MADIILDIRELNKHFGGVHAVQNISFSLEQGELAGLIGPNGAGK
                     TTVFNLITGVYSPDSGAIIFKGEDIASFKTYDVVGRGIARTFQNLRLFQRSTVLENVM
                     TACQQHHRYNFFEAVTHLGNWK"
     assembly_gap    5512..5611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5612..5983
                     /locus_tag="P5541_02830"
     CDS             <5612..5983
                     /locus_tag="P5541_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV70940.1"
                     /translation="EWVFTLFPRLRERKEQHAGTLSGGEQQMLSIGRALMSNPRLLLL
                     DEPSLGLAPIIIRDIFRELKRINEEGVTILLVEQNARQALLLSHRGYVMQTGRILMEG
                     SASDLLNNPEVEAAYLGTAKK"
     gene            complement(6053..6490)
                     /locus_tag="P5541_02835"
     CDS             complement(6053..6490)
                     /locus_tag="P5541_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRV70941.1"
                     /translation="MSRSARIIPVLILGVLLVTALAGLAAAEEKIGVIDPQKVLFQHP
                     KFEQAQRQIKSVVEKKQAEAKDAMDKTTDNNEKQRIFETKRQEAAREEQKIMEPIFKD
                     IDLAIRTVAKAKNVTVVLDKTQVFFGGADLTEDVIQELKKKHA"
     gene            6601..6819
                     /locus_tag="P5541_02840"
     CDS             6601..6819
                     /locus_tag="P5541_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRV70942.1"
                     /translation="MNNCVATFDTTHMAILFEKTCRSAGLDVKIIPVPRHLSASCGLA
                     CRFECEQSEKVRSIASGNSIEVMEYHEI"
     gene            complement(6864..7580)
                     /locus_tag="P5541_02845"
     CDS             complement(6864..7580)
                     /locus_tag="P5541_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70943.1"
                     /translation="MGKNGQGRDPFQGGEKIVLSEDLIAGVTKLLLHLLWPVSCPVCG
                     KVATPACGTCLDGLAQYPLREMCMECMGVFPCPNHRGSVPVRVCNAFRGLPRDLVHMV
                     KYGSRRELGRLMGRSMGRTLVAVDNAFLVPVPLHGGSPRRYNQSLELARGIADIWNAE
                     FGERLEWGITRPTQVGMASGERKKMPRDAILWRGNIPRGKNICIVDDVVTTGATLRSC
                     IEAVRSSGNDVTAALAWAAV"
     gene            complement(7529..8728)
                     /gene="metK"
                     /locus_tag="P5541_02850"
     CDS             complement(7529..8728)
                     /gene="metK"
                     /locus_tag="P5541_02850"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRV70944.1"
                     /translation="MERKYLVSSESVTEGHPDKLADQISDGVLDAILKDDPMGRVACE
                     TIVTTGLVVVAGEITTNCYVDIPRLARNIVKDIGYTRAKYGFDGDTCAVLTAIDEQSS
                     DISCGVDQSLELREGEMSEEKINSIGAGDQGMMIGYACDETEEFLPLPIALAHRLSRR
                     LSQVRKNKTLPYLRPDGKTQVTIEYLDGKAIRVDTVVVSSQHHPAVESGQIRDDIIRE
                     VINPVIPEYLMETAPRILVNPTGRFVMGGPMADTGLTGRKIIVDTYGGVVPHGGGAFS
                     GKDPTKVDRSAAYMSRYAAKNVVAAGLARECKIQVAYAIGVAHPVSLMVETNGTGVIP
                     DCELTALVRENFDFRPAAIIRDLDLRRPQYRLLAAYGHMGRIDLDPLPAWERTDRAEI
                     LSRAAKK"
     gene            complement(8805..9233)
                     /locus_tag="P5541_02855"
     CDS             complement(8805..9233)
                     /locus_tag="P5541_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV70945.1"
                     /translation="MPKETERYLFYDSKNAVTANDLQELYRFTRWGRSRSIEQIDRML
                     QGTSMCFSVRYSRELVAFCRVLTDFVFRASLWDIMVHPDHQGKGLGSSLLDYALDHPG
                     LRDLPLIITYTSELAPFLSRLGFRHEEGAMMLLRKPIEYS"
     gene            complement(9236..9472)
                     /locus_tag="P5541_02860"
     CDS             complement(9236..9472)
                     /locus_tag="P5541_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRV70946.1"
                     /translation="MLIVACQICGEFRIIPGTPDSDGIARARWTCRKCGTGQVVQLPV
                     SVDARGGDLGKILGGMGFSVPVDAVNPGPGDREV"
     gene            complement(9510..10694)
                     /locus_tag="P5541_02865"
     CDS             complement(9510..10694)
                     /locus_tag="P5541_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HRV70947.1"
                     /translation="MDLVMEMNWMLIIILLTVSSIVAYIGDIVGMRFGKKRISIFGLR
                     PRATSSLITVVSGILITILTLAVLSATSQTVRTAIFSMKFVQKQITELTSQLQGSRTE
                     LVDLETRLLDNQKDLVSKQLQLANVEGDLDRSETRLREIEAELERAKAEQQATLSSLE
                     ELQNERSSLEKEVDTLKTESQRLREGLEYIRGGRIVVFAGEMIAQAVILPSSAGTDRS
                     PEAIMENLLREARSSISIRYGVDAEEVRIWIGPESTRKIRDCCIESDARMILRLIASE
                     NTVQGEMIAAEATIHESKKIYERGHVLARETGLPGSLSPEEAEARLYAILTAVNTRAK
                     SDGVLPDPLRGTVGNLGASDFFEAVDALSGKDKNSTVVIRADSDVYSEGPVQVAIQVI
                     DE"
     gene            complement(10815..12179)
                     /gene="lat"
                     /locus_tag="P5541_02870"
     CDS             complement(10815..12179)
                     /gene="lat"
                     /locus_tag="P5541_02870"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HRV70948.1"
                     /translation="MAKVDRTVAAPEAFEEIRKWLLRDGFDIVIDMEKSKGSHMINKI
                     NGEDWLDFYTFFASAPFGMNHPKLDNPEFKETIFKAAINKVASSDIYTEPMAKFVKTF
                     GDIAVPEGFKHVFFIDYGTLAVENTFKVAMDWKVQKLLEKGKISKGDAIKGRRGTKVI
                     HFNEAFHGRSGYTLTVTNTQDPNKYQFFAQYDWPRVINPKIYWPLEENHGVVTWLERT
                     AVKQIKQAIWDNPDDICAIIIETIQGEGGDNHFRTEFFQQLREICDQEEILLIFDEVQ
                     CGMGITGKMWAFEHHAPVKPDMFAFGKKAQICGLVAGPRVDEVENNCFKVSSRINSTW
                     GGTLVDMIRATRYLEIYEEDNVLDYVANTAGPALLDGLRKLQAEFPTLIKNVRGKGLM
                     CAYDFDSGEMRNKALKSFWAKKMLVLPCGDVSIRFRPALNVPLEDLQHALDISKEVFR
                     ECAK"
     gene            complement(12325..12765)
                     /locus_tag="P5541_02875"
     CDS             complement(12325..12765)
                     /locus_tag="P5541_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRV70949.1"
                     /translation="MTGEGLEYKPWWSKGLYFSCLGCGRCCRGDPGAIWVSDEDITAA
                     ARYLEKNEGDFRRSFLTGRWKRPSLREKPNGECVMYSSLTARCMIYPVRPPQCSLFPF
                     WPSLLRSRSDWDRASADCPGMNNGKFFSAEEIESLLLKSPYPDL"
     gene            complement(12806..13846)
                     /locus_tag="P5541_02880"
     CDS             complement(12806..13846)
                     /locus_tag="P5541_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRV70950.1"
                     /translation="MWGTDIGIDLGTANVIIFMKGKGVVLREPSVVAVDQNTDRIVAV
                     GNEAKNMLGRTPGNVVAIRPLRDGVIADYTMTEAMLRFFLKKVITGFARLTRRRVMIC
                     VPSGATDVERRAVLEAAVEIGAKEAFLIEEPMAAAIGANLDISEPRGNMVVDIGGGTT
                     DIAVLSYGGIVVTESLRVGGDRFDENILRFVRKEFNLAIGEQTAENIKMTIGSSCPGQ
                     AEEVMVIKGRDLVHGLPRQIEINDRIIAKSIEETIGMIIEGIRHVLEQTPPELSADII
                     DRGITLTGGGALLKGLPELITRRTLIPAWVADSPLECVALGTGKALEDIDKLRNSGSL
                     IGTSRRRDKRVW"
     gene            complement(13969..14730)
                     /gene="truA"
                     /locus_tag="P5541_02885"
     CDS             complement(13969..14730)
                     /gene="truA"
                     /locus_tag="P5541_02885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRV70951.1"
                     /translation="MKYAAKLSYDGSQFCGWQKQKDIAGATIQDTFEKALAILDGGNV
                     PVTVAGRTDKGVHAAGQVASFSMSRYWEPGKLRNALDANLPESVTVLRVLEVPDNFSA
                     RHSALWREYIYFVWKEHGCPPHIKPYVWKNTSFWDYGRIREACSAIKGEHDFGAFCRL
                     GDRPENSVRTVTKVDFSARGPLCRVRVRGKSFLTNMVRIILGSVDLVGTGKRDISWLE
                     SLLQGKSREEAGPTAPAKGLFLWKVGYGDTSLDRS"
     gene            complement(14727..15542)
                     /locus_tag="P5541_02890"
     CDS             complement(14727..15542)
                     /locus_tag="P5541_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRV70952.1"
                     /translation="MKFLENMTLGQYVPVDSAVHSLDPRCKIVSVLFCLTGVFLAGLP
                     MIFVGWIAFFAVLTILAKIPPRLVASTIRPVWILVVFTTVIHLFFTRGEPLLSWGIIT
                     ITREGAAMAFKMGMRLLLLVMFAGMLTITTSPTELADGLESLFSPFKRFGFPAHELAM
                     MMTIALRFIPTLLNETDRIMKAQTARGADLDRGGPLKRLRAFIPVLVPLFVIVFQRAD
                     DLATAMEARNYSGGEGRTRMNPLDWDGMDSMALSVAVLFSVAAFLAERGQYLR"
     gene            complement(15539..16381)
                     /locus_tag="P5541_02895"
     CDS             complement(15539..16381)
                     /locus_tag="P5541_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRV70953.1"
                     /translation="MSIEVRDLVHIYHPGTPIETRALDRVTFEAPSGAWVAIVGHTGS
                     GKSTLAQHLNGLLRSSEGSVTVDGITIDHGDSSIRSIRQKVGLVFQYPEQQLFEETVF
                     REVSFGPANWGFSEDETRRNVKRSLSIVGIGPELFESSPFRLSGGQKRRVAIASVLAS
                     EPDYLVLDEPTAGLDLGGKRDLVNLLSDLRSLGKGIVHITHDLDLALRLADIVLVLDK
                     GKSVLWGSPAVILEELAERDISGLVVPPVVRFASRLREVGLDVPLTWEPERLARAICA
                     GVKK"
     gene            complement(16372..17199)
                     /locus_tag="P5541_02900"
     CDS             complement(16372..17199)
                     /locus_tag="P5541_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRV70954.1"
                     /translation="MTGDCLASFTGVSYTYPGSENQAISSIDLEIKRGEWIAVLGSNG
                     SGKSTLAKHLNALLVPSQGDCFVNGLNTRNDEGRKEARRSVSMVFQNPENQIIAAVVE
                     EDVAFGPENMGLPSEEIRKRVRWALKISGLVDKARKPVYALSGGQKQRLAVAGAIAQK
                     PPCLVMDEATTMLDPRGRDDLMKILSALHSGGMTLVSITHRLEEILECDRCIVLSGGR
                     NVWQGTPRELFRQGKRLQTWGLEVPGIIREWEMLVEGGALKADTPTEIEAMVEELCP"
     gene            complement(17196..17555)
                     /gene="rplQ"
                     /locus_tag="P5541_02905"
     CDS             complement(17196..17555)
                     /gene="rplQ"
                     /locus_tag="P5541_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRV70955.1"
                     /translation="MRHRVDRRKLGRYGSHRMAMLSNLTASLFLEEQVETTLTRAKEL
                     RRVAERLITKAKKGDLHNRRLVASRMGNVKALQKLFGDIAGRFGERQGGYTRIIKTGY
                     RHGDAAPMAIIELVERS"
     gene            complement(17552..18541)
                     /locus_tag="P5541_02910"
     CDS             complement(17552..18541)
                     /locus_tag="P5541_02910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRV70956.1"
                     /translation="MEQIRPDIRVEESTPLSGKIVLGPLDKGYGVTLGNALRRVLLSS
                     IRGAAVTAVRIEGILHEFSTIPGMREDVIELLLNLKNLAIISHSGEPRVLRLEATGPG
                     AVTPQGIIPDSDIEFVDPEMYICTLEDGATLEMDLFVESGTGYVTSERERPDDIPLDA
                     IVVDAVFSPVKRVKYEIQDARVGQRTDYERLVLEIWTNGSVSPEEAATEASGILQHFF
                     AMLVQGLGSDEQGGWAGQIQQPDPEPVTDDGALKRPIKDLELSIRSENCLLRGGIQTI
                     GDLIGRNKAELLKIRNLGKISLREIEEKLDPFGLSIKGEEPEDEDDLKEDEAR"
     gene            complement(18564..19196)
                     /gene="rpsD"
                     /locus_tag="P5541_02915"
     CDS             complement(18564..19196)
                     /gene="rpsD"
                     /locus_tag="P5541_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRV70957.1"
                     /translation="MSRYTGAVCRLCRAEGTKLFLKGDRCYTEKCAINKRNSKPGQHG
                     QSRRRGKESEYGIRLREKQKLRRFYGINETQFRRFYASAVKMAGQTGHNFLQILERRL
                     DSVVYRLGFGVSRSQARQFVRHGHLLVNGQKVDIPSYLVKAGDVISVDSGSRDSAVLK
                     ENLEVAASRSVPGWLEFNAEKQEGHIVSLPSREQIEVPVKEQLVVEFYAR"
     gene            complement(19227..19619)
                     /gene="rpsK"
                     /locus_tag="P5541_02920"
     CDS             complement(19227..19619)
                     /gene="rpsK"
                     /locus_tag="P5541_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRV70958.1"
                     /translation="MAKRIQRRGKRKEKKNISYGVAHIFSTFNNTIISVTDRQGNLLS
                     WASGGNVGFKGARKSTPFAAQMAAQQVAKQVQDHGMLEIDVIVRGPGPGRESAIRSLQ
                     AAGLQVNAIKDETPVPHNGCRPPKRRRV"
     gene            complement(19667..20050)
                     /gene="rpsM"
                     /locus_tag="P5541_02925"
     CDS             complement(19667..20050)
                     /gene="rpsM"
                     /locus_tag="P5541_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRV70959.1"
                     /translation="MARIAGVDIPREKRIVIALTYIFGIGPTTAKKILKATSIDPDTR
                     VKDLTEEEAQKLRAEIEESCKVEGDLRREITMNIKRLIEIGCYRGMRHKFGLPVRGQK
                     TKTNARTRKGPRRAVAGKKKPAAKK"
     gene            complement(20057..20182)
                     /gene="rpmJ"
                     /locus_tag="P5541_02930"
     CDS             complement(20057..20182)
                     /gene="rpmJ"
                     /locus_tag="P5541_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRV70960.1"
                     /translation="MKVKPSVKKVCEHCRIIKRNGIVRIICSKNPRHKQRQGARR"
     gene            complement(20252..20470)
                     /gene="infA"
                     /locus_tag="P5541_02935"
     CDS             complement(20252..20470)
                     /gene="infA"
                     /locus_tag="P5541_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRV70961.1"
                     /translation="MPKDDMIEVKGKVLEPLPNAMFRIELENGHKVLGHVSGKMRMHF
                     IRILPGDRVLVQISPYDLTRARIVYRYK"
     gene            complement(20604..21374)
                     /gene="map"
                     /locus_tag="P5541_02940"
     CDS             complement(20604..21374)
                     /gene="map"
                     /locus_tag="P5541_02940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRV70962.1"
                     /translation="MISLKNETDLVHLRKAGKIVADVLMMIGDIVRPGLDTLTLDERA
                     EEYILSSGGKPAFKGYRVPGIPRPFPGTLCVSVNEEIVHGIPARERRLEEGDIVSVDV
                     GVSIEGYYGDAAYTYPVGDISPERADLVDVTRESLQRAIQAAREGATLGDIGFSVESF
                     VIPRGYGIVRDYAGHGIGRNLHEPPQVPNYGSPGRGVTLKSGMTLAIEPMIMSGSERV
                     RTLEDKWTVVTADGSDAAHFEKTIVVTGGDPEVITPWQ"
     gene            complement(21374..22021)
                     /locus_tag="P5541_02945"
     CDS             complement(21374..22021)
                     /locus_tag="P5541_02945"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRV70963.1"
                     /translation="MKLIFLGSPGAGKGTQAAVLRERQDIAHISTGDILRENVKNSTP
                     LGNKAREYMDSGKLVPDDLIIDMMEDRLARPDCAKGFILDGFPRTVPQAEALDHLLGR
                     MGLELDGVILFEVPEELVVQRLTGRRICGQCGEIYNIRFKDTKVPGVCDRCGGVLQQR
                     DDDREEVVHRRLEVYEEQTAPLIDYYETKGNLVRVDASQDGDRVVKDIEKAVGKR"
     gene            complement(22037..23332)
                     /gene="secY"
                     /locus_tag="P5541_02950"
     CDS             complement(22037..23332)
                     /gene="secY"
                     /locus_tag="P5541_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRV70964.1"
                     /translation="MLDAFRDAFRLPDLKRKILFTLGVLFLFRLGTHIPTPGVDAEAM
                     SQLFEQGGILGFLDLFAGGALRRFSVFALGVAPYINASIVMQLLVVVFPALEKLQKEG
                     EQGRKKIVQYTRIGTVFFALVQAVGMTFWLGNLGIFTGGALGFIAAIVTVTAGSLVVM
                     WLGEEISDHGIGNGISLLIFAGIVARLPEAIVRTWSLMTMGEMSVLVITLAVVLMIIV
                     IAGCVLLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQAGVIPIIFASSVLLFPS
                     TIVRFFSGDISGVIQRLLAPDSFVYMALYVLLIIFFSYFYTAVVFDPADTANNMKKYG
                     GFVLGIRPGKPTADYIEKIMSRITLGGAVFLAGVALLPTLMTRMMGITTFYFGGTAVL
                     IVVGVALDTVHQIEAQLLMRHYEGILKRSKGKTSGLLKF"
     gene            complement(23333..23779)
                     /gene="rplO"
                     /locus_tag="P5541_02955"
     CDS             complement(23333..23779)
                     /gene="rplO"
                     /locus_tag="P5541_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRV70965.1"
                     /translation="MELHELRPAPGSKRKPKRLGQGAGSGTGKTAGKGHKGQKARSGG
                     GVRPGFEGGQMPLSRRTPKRGFSNARFKKSFQVVNIERIEDAFEAGSEVGIPEMLLAG
                     LISNDHLPVKVLGKGEISRAITVKANAFSKQAAARIEAAGGKIEVV"
     gene            complement(23792..23977)
                     /gene="rpmD"
                     /locus_tag="P5541_02960"
     CDS             complement(23792..23977)
                     /gene="rpmD"
                     /locus_tag="P5541_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRV70966.1"
                     /translation="MAKVSIKLVKSTIGRSPRQARTVVALGLRKMGHTVTHEDTPQIR
                     GMIDTVSHLVVWENVAE"
     gene            complement(23991..24503)
                     /gene="rpsE"
                     /locus_tag="P5541_02965"
     CDS             complement(23991..24503)
                     /gene="rpsE"
                     /locus_tag="P5541_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRV70967.1"
                     /translation="MRGNNGGPKGQEMAERVVSINRVSKVVKGGKRFKFSVLVVVGDE
                     AHNVGIGIGKAREISEAIRKGIDKAKKNQVELKKVGRSIPHDIIGHFGAAEVLLKPAA
                     PGTGVIAGAVVRAIMELGGVSDVLTKVIGRTSNPVNVAYATMQALKGLRTPEEVMRLR
                     GKDIRQAQQV"
     gene            complement(24518..24883)
                     /gene="rplR"
                     /locus_tag="P5541_02970"
     CDS             complement(24518..24883)
                     /gene="rplR"
                     /locus_tag="P5541_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRV70968.1"
                     /translation="MKKRTRNDMRLIRHRRLRRTLSGTGERPRLSVFRSLNHMYAQLI
                     DDTRGHTIAAASTLEKDIRSNSNGDGDFEAAKSVGKLIAQRAIEKGITQVLFDRGGHM
                     YHGNVKALADAAREAGLKL"
     gene            complement(24904..25440)
                     /gene="rplF"
                     /locus_tag="P5541_02975"
     CDS             complement(24904..25440)
                     /gene="rplF"
                     /locus_tag="P5541_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRV70969.1"
                     /translation="MSRIGRKLIGMPAGVTLAVEESKVKVKGPKGELSVDLVAGIDVA
                     VEDGSVKVTRQNDEKKTRAFHGMVRALVNNMITGVSQGFERRLEIVGVGWRAQMQGKT
                     LVMNLGFSHPVEFEPPSGIEISTDGPTKITVTGIDKQAVGHTAAIIRGFRPPEPYKGK
                     GIRYAGESVSRKAGKAGA"
     gene            complement(25453..25857)
                     /gene="rpsH"
                     /locus_tag="P5541_02980"
     CDS             complement(25453..25857)
                     /gene="rpsH"
                     /locus_tag="P5541_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRV70970.1"
                     /translation="MYVNDPVGDMLTRIRNANMVYHETVDMPLSKVKLAIAKILKEEG
                     YVRNFKVVNDPKKPYATLRLFLTYGPQRERVIQGLRRISKPGRRIYVQKDELPSVLKG
                     LGTAIISTSKGMMPDSEARTHGLGGEVVCYVW"
     gene            complement(25887..26072)
                     /locus_tag="P5541_02985"
     CDS             complement(25887..26072)
                     /locus_tag="P5541_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRV70971.1"
                     /translation="MARKAMRIKAAREPKFKVRAYTRCPICGRVHAYMRQFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(26090..26632)
                     /gene="rplE"
                     /locus_tag="P5541_02990"
     CDS             complement(26090..26632)
                     /gene="rplE"
                     /locus_tag="P5541_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRV70972.1"
                     /translation="MMPRLADMFKNEITGRLQKQFGYSNIMQVPRVQKVVVNIGVGEA
                     KVDYKYMEASISELRTITGQQPMMKRAKKSVAGFKLREGMPVACSVTLRGSRMWEFLD
                     RLISVALPRIKDFQGLSAKGFDGRGNYNLGLREQLIFPEIEYDKVSRLRGMNISIVST
                     ARTDEEAMSLLQALGMPFNR"
     gene            complement(26648..26974)
                     /gene="rplX"
                     /locus_tag="P5541_02995"
     CDS             complement(26648..26974)
                     /gene="rplX"
                     /locus_tag="P5541_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRV70973.1"
                     /translation="MSKMRIRKGDRVHVISGKDKGKEGKVLRHFPVREMVLVEGVNIV
                     TKHVKPSAKNPQSGIVKQEAPIYSSKVKLVCTACGAATRVGRAFLENGRKVRVCKKCG
                     EIIDKV"
     gene            complement(26988..27356)
                     /gene="rplN"
                     /locus_tag="P5541_03000"
     CDS             complement(26988..27356)
                     /gene="rplN"
                     /locus_tag="P5541_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRV70974.1"
                     /translation="MIQLRTKMKVADNSGAKKIMCIQVLGGSKKRYASVGDIVVASVK
                     EAIPNSNAGKGDVVKAVIVRTKKEIRRRDGSYVRFDDNAAVLIDNNGDPRGTRIFGPV
                     ARELREKRFMRIVSLAPEVV"
     gene            complement(27360..27650)
                     /gene="rpsQ"
                     /locus_tag="P5541_03005"
     CDS             complement(27360..27650)
                     /gene="rpsQ"
                     /locus_tag="P5541_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRV70975.1"
                     /translation="MDEKVISRKTRVGTVVSDRMSKTVVIKVDRMAKHPLYGKPVLRS
                     KKFMAHDEEESCRIGDRIRIEETRPLSRCKRWKVVEIIERAPVLGGSQEKGA"
     gene            complement(27663..27869)
                     /gene="rpmC"
                     /locus_tag="P5541_03010"
     CDS             complement(27663..27869)
                     /gene="rpmC"
                     /locus_tag="P5541_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRV70976.1"
                     /translation="MNSRELRELSLEELSDKHKMFKEELFNLRFQHAIGQLSNTGRIG
                     DVKRSIARVLTVMREKEIGPGRPA"
     gene            complement(27872..28294)
                     /gene="rplP"
                     /locus_tag="P5541_03015"
     CDS             complement(27872..28294)
                     /gene="rplP"
                     /locus_tag="P5541_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRV70977.1"
                     /translation="MLMPKRVKYRKPFRRPLSGMAKGGKTVAFGEYGLQALECAWITA
                     RQIEAARVAISRKMKKGGKTWIRIFPDHPFTKKPLETRMGKGKGNPEFWVAPVKRGRI
                     MFEISGVSREIAEQAFLTASHKLPIKVRLVSRDGMGGE"
     gene            complement(28301..28972)
                     /gene="rpsC"
                     /locus_tag="P5541_03020"
     CDS             complement(28301..28972)
                     /gene="rpsC"
                     /locus_tag="P5541_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRV70978.1"
                     /translation="MGQKVHPVGYRLGIVRDWESRWYAGGRDYAAKLHEDLRLRKWIM
                     KRWANAGVAKVEIERIGKVIRFTIWTARPGVVIGKGGTEIQKVREELQELTGGKVMIN
                     VQEIKNPDKEAQVVAEGVASALERRVSFRRAMKQSIFRATKAGAEGIKIQCSGRLGGA
                     EIARTEWYLEGRLPLSTLRADIDYGFAEARTIYGIIGIKVWIYKGQAVRPAPAGIQQD
                     TAERR"
     gene            complement(28986..29318)
                     /gene="rplV"
                     /locus_tag="P5541_03025"
     CDS             complement(28986..29318)
                     /gene="rplV"
                     /locus_tag="P5541_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRV70979.1"
                     /translation="MEARAVARQVRVSPTKSRQVLNLIRGKKAGKALDELRFTPKDAA
                     RVVEKVLKSAIANAEHNYGMDMDRLVIVRAYADQGPSMKRWQPVSMGRVHGYKHRTSH
                     ITVVVAER"
     gene            complement(29331..29612)
                     /gene="rpsS"
                     /locus_tag="P5541_03030"
     CDS             complement(29331..29612)
                     /gene="rpsS"
                     /locus_tag="P5541_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRV70980.1"
                     /translation="MARSTKKGPFIEQKLLRKVEAMNEEGKKKVIKTWSRRSTIVPDM
                     IGHTIAVHNGRTHIPIFISENMVGHKLGEFAPTRKFGGHAGQERHTRVR"
     gene            complement(29631..30458)
                     /gene="rplB"
                     /locus_tag="P5541_03035"
     CDS             complement(29631..30458)
                     /gene="rplB"
                     /locus_tag="P5541_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRV70981.1"
                     /translation="MGIKKYKPYTPGRRTMTTSSFEEITKKEPEKSLVESLKKTAGRN
                     NLGRVTMRHRGGGAKRLYRIIDFKRDKLEVPGKVAAIEYDPNRSARIALIHYADGEKR
                     YILAPVGLGVGDTIVAGPDSDIRPGNALKLRDIPVGTFIHNIELEPGRGGVMVRSAGT
                     SAQLMAKEGKKAFIRLPSGELRLALLECMATVGQIGNEDHENLTSGKAGKTRWLGRRP
                     HVRGMVMNPVDHPLGGGEGRSKSHKHPVSPWGTPAKGYRTRKRKPSDKNIVRRRFDK"
     gene            complement(30476..30772)
                     /gene="rplW"
                     /locus_tag="P5541_03040"
     CDS             complement(30476..30772)
                     /gene="rplW"
                     /locus_tag="P5541_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRV70982.1"
                     /translation="MKEMPQDIIIRPVITEKSSRMMEQNKYTFEVHPRANKIEVRDAV
                     VELFKVKVVKVSTIHMRPKPKRMGAFLGKSRSWKKAVVTVAAGERIEFFEGANA"
     gene            complement(30769..31395)
                     /gene="rplD"
                     /locus_tag="P5541_03045"
     CDS             complement(30769..31395)
                     /gene="rplD"
                     /locus_tag="P5541_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRV70983.1"
                     /translation="MPTVKVMNFQGEVIGEENLSEAVFAAPVHVPAMHQVVVAQQANA
                     RQGTHSTKTRADVRGGGKKPWKQKHTGRARHGSRRSPIWVGGGITHGPHPRDYHQKVN
                     RKVRRLAIRSALSLKAGEGSLILLDRIDMEKPSTKMMVSFLNVAEASKKPLVMVSESN
                     VAVSRSAGNIPGLMLLHVDSINVYDILNHRHLILTLDAARKIEEVYGA"
     gene            complement(31411..32049)
                     /gene="rplC"
                     /locus_tag="P5541_03050"
     CDS             complement(31411..32049)
                     /gene="rplC"
                     /locus_tag="P5541_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRV70984.1"
                     /translation="MSMGILGRKLGMTQIYDEKGHSVPVTVIEAGPCTVVAVRTMEKN
                     GYTSVSLGFGKVKAEKLPRPYRGVFEKKGLEPTRWIREFRVDDTGEYEPGKVVSVDLF
                     QEGDTVDVTGKSKGKGFAGVIKRHHFQGGPGSHGASKFHRQPGSAGASSYPGHIFKGK
                     TMPGRMGGERVTVKNLSIAGVDIENNLLLVKGAIPGPRNGLVMIRKQDGAAR"
     gene            complement(32089..32394)
                     /gene="rpsJ"
                     /locus_tag="P5541_03055"
     CDS             complement(32089..32394)
                     /gene="rpsJ"
                     /locus_tag="P5541_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRV70985.1"
                     /translation="MAKKIRIKLKAFDHRALDSSAFQIAETAQRTGAKVSGPIPLPTE
                     INKFTVLKSPHKDKDAREQFEMRTHKRLIDIIDPTQKTMESLMQLNLPSGVDIQIKL"
     gene            complement(32416..>32483)
                     /locus_tag="P5541_03060"
     CDS             complement(32416..>32483)
                     /locus_tag="P5541_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70986.1"
                     /translation="FAVREGGRTVGAGVVTEILDN"
BASE COUNT         6821 a         9598 c         7721 g         8243 t
ORIGIN      
        1 tcctctgggc atcctgggca tgataatcgg aatgtccatg agaagaatgg ggatcgtgac
       61 cgaaccggcc tcggcccttt cccattttat agctacaggg tttcatcccc ttgtcggggg
      121 ggtactctgg ggcgggatcc tgataaccgt gatcgggaca gcggctggac tcactttagg
      181 ggtggccgtg aacatcgtct cggatatcct ggccccacgt ttcccaggcc tctgcggggg
      241 aaattcgtca gccacggcaa ccagatgtgc cgtgctagca ctgatccttg ccgcgggtct
      301 tgctggaatt gcaggacagg gctcgatgat actgaagtgg agttacctta gcatggggct
      361 gagaggatcg ggaatatttt tccctcttgt tgcctcggtt cttttcccgg ggaggctgtc
      421 tccgggaatg gcagcgatct ctggcggagc cgggcttctt gccaccatca tgtggcccct
      481 gtccggtctg cccctggagc cactttttgc agggctttcg gtttcggcgg ctgtcgtctt
      541 tctcgggata cgttacggca ggagagaaca tcccctgccg taacagagtc aggacctacc
      601 tcttgtagcc aataatattt ctcagcaggt ctttcctgtc ttttacatcg gcctcgctct
      661 ccactcctgt cggagagttg ccgtcgatga caccggcgat cccccgtccc tgttcgctgc
      721 caaaaacgag aacctgcaga gggtttgccg tcgcggcaaa tatcctgcat acctcctgga
      781 cctgcttgat cccgttgaga acgcttatgg ggaaaccgtt cctcatgagg atgacaaacg
      841 agtgtccggc acccagcgca agggcattat ccctcgccgc ctcgacaagg tctgatgaat
      901 taccctccga cctgaccagg catggcccgg aggcctcgca aaaggcaagg ccaaattcaa
      961 gtaccgtcga tgatgtcgca agaacctcga aaagatcctc gacggtcttg atgaaatggc
     1021 tctgtcccag gattacattg catccctcgg gaatttcaat atccaccagg tcccatgcta
     1081 ctgtttttcc ggccattgtc ccaacctcct ccatgaataa tgaagttttt acttccttcc
     1141 ggatccttcg ctcacggatt cggacaggat ctcttccagt ggctccagaa tatgcgcaaa
     1201 accacctgcc gaagccggcg gaagaactat atgggcgacc tccctcaggg acgctctggc
     1261 atcatccatc gttatcgcta tgtcggcaaa cctcagcatc tccagatcat tcaggtgatc
     1321 ccccgccgcg gccacgatcg aaggccgttc gctttcctgc atgaggctga tcatcttttg
     1381 aagggcagcc cccttcgaga cccctgaagg aaggatgtca agaaaaccgt ttccggccat
     1441 gacggcacga cattcccccg cgagccgcga ggccccgacc ctttgtcccg ctgactcgac
     1501 ctcacccggg tccccataca ggataacacg gtaaacacct tctatcggtt cggggaacag
     1561 aagcccttca tccactggca cgccgatctt cgagaagtat tccgccgcca ggtcgtcccc
     1621 ttttttacac aggacattct catcacggaa tacctgtatt ccccatggaa tgtcccacag
     1681 catttccaga acatccttca ctacgcgggg atccagtttc tgtccccaga tctcgcggcc
     1741 cgtggccgcg tccatgaccc tggcgccgtc gtaaacaatg gtaatcgacg aggcaccggt
     1801 ccttataata tgtttttttg cgcttccgag gatcctcccc gtggcaagta ccatgtccca
     1861 tccccttgcc acaagcgcgt gacatgcatc ggcaacaact tccggaacat ggtcatcgtg
     1921 agcgagcgtt ccatcgatat cggcgacgag caattttttc atgctatacc ccccgggagc
     1981 aggtctgcct gcccttttcc tgttcattta atactaccat gggactggtg ctgaacataa
     2041 cctttttcct cttgattgtt atccaattgt ggtaacatat tcatatttcc ataactattt
     2101 tcaggaggtg taggttttga ggaagatcct tgttctggca ttgcttgcag tttttgcggt
     2161 ttcaggtttt gccgcgccag ctctttccgc ggatgtgatc aaggtgggct atctcgccgc
     2221 ccttacgggc gactgggctg cctacggaca gaccgagctc aatgcggcca aacttgccgt
     2281 cgccgagatc aacgccaagg gtggagtact tgggaaacag atcgagcttt tcccctatga
     2341 cttccgcacc aggcccgagg acgccgtcaa cgccttcagg cgcatggccg agaacgacaa
     2401 ggtcgtggcc gtagtgggag ccaacggaag cggcatcaac atcgccaccg caccactcgc
     2461 caacaagtat ggcattcccc agataggaac ggtctctacc aaccttctcg tcactgtaaa
     2521 tgaaaaggga gaactgaacc ccttcatgtt ccgcacctgc ttcaccgacc cgtaccaggg
     2581 tcaggccatt gccgttttca cagcgaagga gctcaagaaa aagacagccg ccgtccttta
     2641 tgacgtcggc agcgactact cccaggggct tcgcgaattc ttcatccagt acttcgaaga
     2701 atacggcggc aaggtcgttg ccgaccaggg cttcagggcc ggcacagacg tagacttccg
     2761 cgcacagttg accacaattc gcgacagcaa ggctgaggtc ctcgtcctcc ccaacatggg
     2821 caaggaaatg gccctcatca tgaagcaggc acgcgaactc ggcatggatg atatcgtatt
     2881 cgtcggaggc gacggttacg gcgagttcat gtgggagatc gcaggagacg ccatggagaa
     2941 ctcctactgg atcaaccatg tcgctcccga agacccagca atggccgact tcttcgccaa
     3001 ctacaagaag acctacaacg acgagtgcaa ggagttcgtc aacgggatcc tggcctacga
     3061 ttctatctat tggctggccg acgccatcga gagggccggc aaggtcgaca gcaccgccat
     3121 cagggatgct ctcaggaaca ccgtcaatgt acagctccat cacgccgtta tgtcgatcga
     3181 ccccgaaact cacaacccga agaacaagac cgccgtcgta cttgtcgcca aggacggacg
     3241 ggcccagttc ttcacgaagg tcacacctga atagaaactc ccctgtttca ggcaacgatt
     3301 ctgacagggc ggacgccgta acaggcgtcc gccctgtagt tgcagcatat taaggatggt
     3361 gaacccgttt tggaaactct acttcagcag gtgatcaacg ggctttctct gggttcggtt
     3421 tatgctctta tcgcagtcgg atattccctg gtttattcga tacttctctt ttcgaacttt
     3481 gcgcacggcg gatttcttgt cataggtgga tacatctgct actacactct ggcagccgtg
     3541 ggccataaca tctggatagc aagtcttctg gccatggtcg gcgcaggcgt tgcagcggta
     3601 atggtcgagc gccttgccta caaaccgatc cgtgagagaa ccaacgtcac tctatacctg
     3661 ctcatagctt ccatgggaat gagcatcgtt atcgagaata ttttcgttgt gaccgtagga
     3721 ggccgcttca gggcacttcc accggttatt ccgatgaacc cggtaagtgt cttcggcatt
     3781 gctaccacaa gtgccttcga tatcctgtcg cttgttatgg cagtcctttt ccttacggga
     3841 ctccagatct tcctttccag gaccaaatgg ggacttgcca taagggcagc ctcctacgat
     3901 ctgcggactg ccgggcttat gggcgtaaat gtcaacaaac ttatctccat cgtatttttc
     3961 gttgccgggt tgcttgccgc ggtcgggggc atctttctct cggtccggta caccctttac
     4021 ccccagttgg gcctgataac aataaaggct ttcgtggcag cagtcattgg gggccttggg
     4081 agccttccgg gagccgtggt cggcagcctt atcctcgggc ttgccgagat gctgacggcc
     4141 gggttcatat cgagtcagat gagagatatt gttgttttcg gtctgcttat tttaacgctt
     4201 ctgtggagac caaccggcct ctttggcaag cacgtctccg aaaaggttta ggggaggtgc
     4261 aatatgtcag gatatactga aggcatcatc attctcatct gcatcaattc catagcggca
     4321 atgggcataa gcctccttac cggattcacg ggaatattca gcctcggtca cgctggatat
     4381 atggcgatag gagcctatac ggcagcgatc ctctcagttc gccatgatgt gccgtggata
     4441 gtggccgtgg ttgccggagg aatgctggcg atgctgatcg cgtggctcat cggtattccg
     4501 acactaaggc tgatgggaga ctattacgca atcgcctcca taggtcttgg agaggctata
     4561 cgtcttgttc ttgagaactg gcagtccttt acgcgagggg ccagaggatt ccccggcatt
     4621 gataatttca caacgcttcc cgtcgctgca gggtttttta ttggaatgct cgttcttatg
     4681 tggtttttga taagcgggaa ctatgggcgc gctttcaagg cctgcaggga cgactacata
     4741 gcagcatccc ttcttggttt caagacggcc cattaccggg tcttgagcct ggcaatatcc
     4801 ggattctact gcgggatagc cggagcgctc atggccgggt tcctcacctt tcttcagccc
     4861 atcatgtttg accttaacaa atcgacagaa ctcacggccg tcgtcgtatt cggaggcctg
     4921 ggatctctca gcggctcaat aatcggcacg accgtcattg gaatagtaac tgaggttttc
     4981 cgccccatat cccagtacag gatgctcatt tacggcgcca taatggtcat cataatggtc
     5041 atgcgacccg agggaattat gggacagcac gaactcggcc ccaggctttt gcgctccctc
     5101 ttctccagaa aacctgcggg gaaggagaat gctaccaatg gctgacatta ttctcgacat
     5161 cagggaactg aataagcact tcggcggagt acatgctgta cagaacatat ccttctctct
     5221 ggaacaggga gaacttgccg ggctgatagg ccccaacggt gccggaaaga caactgtctt
     5281 caacctgata accggagttt attctcccga ctcaggggcg attattttta agggagagga
     5341 tatcgcgtcc ttcaagacat acgacgtggt cgggagaggt atcgccagga cattccagaa
     5401 ccttcgcctc ttccagagat ctaccgtcct tgaaaacgtc atgacggcat gccagcagca
     5461 tcacaggtac aacttcttcg aggctgtgac tcacctggga aattggaaaa annnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagtgggtt ttcactcttt ttccacgcct
     5641 gagagaacga aaggagcagc acgcgggaac cctttccgga ggagagcagc agatgctttc
     5701 cattggaagg gcacttatgt ccaaccccag gctgcttctt cttgatgaac catctctcgg
     5761 tcttgcaccg ataattatcc gcgacatttt ccgtgaactg aaaaggatca acgaagaggg
     5821 agttaccatc cttctggtcg agcagaacgc gcgccaggca cttttgcttt cccacagggg
     5881 atatgtaatg cagacaggga ggatcctcat ggagggttct gccagtgacc ttctgaataa
     5941 cccggaggtc gaggccgcgt atctgggaac agccaagaag tagcgtttca ggcaattttg
     6001 cggatgaaaa agcccgggcc tcaaggcccg ggctttttca gtacaatact tgctaggcgt
     6061 gcttcttttt gagttcctga ataacgtcct ccgtaaggtc ggcacctccg aagaacacct
     6121 gtgtcttgtc aaggaccacg gtcacgttct ttgccttggc taccgtcctg atagcgaggt
     6181 ctatgtcctt gaatatgggc tccattatct tctgctcttc gcgagcagcc tcctggcgtt
     6241 tcgtctcgaa aatacgctgt ttctcgttgt tgtcagtggt cttgtccatt gcatccttgg
     6301 cttcggcctg tttcttttcg accacgctct tgatctgtct ctgcgcctgc tcgaatttgg
     6361 gatgctggaa cagaactttc tggggatcga taacaccgat cttttcctcg gcggccgcca
     6421 ggcctgccag ggccgtaacg agaagaacac ccagtatcag aacgggaatg atccttgctg
     6481 aacgcgacat aaaagaacct cctttgattt cagtcaatca ggtaaatatt ttatccttac
     6541 cagcgggccc cgtctaggta taattacata tataccgctg aacccattgg aggaatcgat
     6601 ttgaacaact gcgttgcaac ctttgatact acccacatgg ccatcctttt cgaaaagacc
     6661 tgccgaagcg cgggactgga tgtaaagata atccccgtcc ccagacatct ctcggcgagt
     6721 tgcggcctgg catgccggtt cgagtgtgaa cagtccgaaa aggttcgttc catagcctcc
     6781 ggtaattcga tcgaagtcat ggaataccac gaaatatgaa agatcctgaa aaaactccgc
     6841 cacggtgacc tttgccgggg cccttacact gcggcccagg ccagggccgc agtgacatca
     6901 tttccggatg acctcacggc ctcaatgcaa ctcctgagtg tggctccggt ggtaacaaca
     6961 tcatcaacaa tacatatgtt ttttccccgc gggatattac ctctccataa aatggcatcc
     7021 ctgggcattt ttttcctttc ccccgacgcc atacctacct gggtgggtct ggtgatcccc
     7081 cactccagac gttctccaaa ctcggcattc cagatatctg cgatccctct ggcaagttcc
     7141 agggactggt tatacctcct gggacttccc ccgtgcaagg gaacgggaac caaaaaggcg
     7201 ttatcgacag caaccagggt tcttcccatg cttcttccca tgagcctgcc gagttccctg
     7261 cgagagccgt actttaccat gtgaacaagg tccctcggga ggcccctgaa ggcgttacag
     7321 acccttacag ggacactccc tctgtggttt gggcaaggaa agactcccat gcactccata
     7381 cacatctccc tcagcgggta ctgcgcaagc ccgtccaggc aggtcccgca ggcgggcgtt
     7441 gcgactttcc cgcaaacagg acaggacacc ggccacagta gatgcagtag cagtttcgtg
     7501 accccggcaa ttaagtcttc agacaggact attttttcgc cgccctggaa aggatctctg
     7561 ccctgtccgt tctttcccat gcgggaagcg gatccaggtc gatccttccc atgtggccat
     7621 aggcagcaag aagcctgtac tggggcctgc gaaggtccag atccctgatg atcgctgccg
     7681 gcctgaaatc gaaattctcc cttacgagag ctgtgagttc gcagtcgggg atgacccccg
     7741 tgccgttggt ctcaaccata agggataccg gatgagccac acctattgcg taggctacct
     7801 gaatcttgca ttcgcgcgca aggcccgcgg cgacaacgtt cttggccgca tagcggctca
     7861 tgtatgctgc cgaacggtcg acctttgtgg gatcctttcc cgagaaggct cctccaccgt
     7921 gagggacaac tcctccgtag gtgtctacaa ttatctttct tccggttagc ccggtgtcgg
     7981 ccatgggtcc ccccattacg aatctccccg tcggattgac gaggatcctg ggagcagttt
     8041 ccatcaggta ctcaggaata acagggttta ttacctcgcg gataatatca tccctgatct
     8101 gtcccgactc aacggcgggg tgatgctgtg aagagaccac cactgtgtcc actcttatcg
     8161 ccttgccgtc aagatattcg atcgttactt gcgtctttcc atcggggcgc agataaggca
     8221 gagtcttgtt cttgcgaacc tggcttagtc ttctggaaag cctgtgagca agagcgatgg
     8281 ggagagggag aaattcttct gtctcgtcgc aggcataccc gatcatcatc ccctggtcac
     8341 cggctccgat actgttgatt ttttcctccg acatctcgcc ttcacgcagt tccagcgact
     8401 ggtccacccc gcagctgata tcggaggact gttcgtctat ggccgttaat acagcgcatg
     8461 tgtcaccgtc aaaaccgtac ttggccctgg tgtaaccaat atccttgact atgttccttg
     8521 ccagtcgggg aatatccaca tagcagttgg tggttatctc tccggctact accacaaggc
     8581 cggtagttac aatggtctcg caggcaaccc ggcccatggg gtcatccttg agtatcgcgt
     8641 ccagaacacc gtcggatatc tggtcggcca gtttgtccgg atgtccctct gtcacagact
     8701 cggatgatac aagatacttt ctttccaatt accttccacc tccctggttt tttgggcaga
     8761 tcttccgatt cctgcccgta aacggatagt tcctcatgat cgtttcatga gtattctatc
     8821 ggctttcgaa gaagcatcat tgctccttct tcgtgcctga accccagtct cgaaagaaaa
     8881 ggggccagtt cgctcgtgta ggtaattatc agcggcagat ccctcaggcc agggtgatcg
     8941 agagcataat caagaagcga agagccaaga ccctttccct ggtgatccgg atgaaccatt
     9001 atatcccaaa gggacgccct gaaaacaaaa tccgtaagga cccggcagaa agcgaccagt
     9061 tctctgctgt atctcacaga gaagcacatg gaagtcccct gaagcatgcg gtctatctgc
     9121 tctatcgaac ggcttctccc ccacctcgtg aaccggtaca gttcctggag gtcgtttgcc
     9181 gtaacggcat tcttgctgtc gtagaaaagg tacctttccg tctctttcgg cattgtcaga
     9241 cttccctatc ccccggtccc gggttaacag catctacagg aacggaaaaa cccattcctc
     9301 caagtatctt ccccaggtcc cctccccttg cgtcaacgct gacagggagc tgtacaacct
     9361 gacccgtgcc gcatttgcgg caggtccacc tggctcttgc gatgccatcg ctgtcgggag
     9421 tccccggaat aatcctgaac tctccgcaaa tctggcaggc tacgataagc attctgtttt
     9481 tcgcctttcc gggtcacctg ggagaaattt cattcgtcga tcacctggat cgcgacttga
     9541 acgggaccct ctgaataaac atctgaatct gcccttatca ccaccgtgga atttttgtct
     9601 tttccagaaa gggcatctac ggcctcgaaa aaatcagagg caccaaggtt accgacggtc
     9661 cctctcagtg gatccggaag gactccatcg ctttttgcgc gggtgttgac tgcagtgagt
     9721 atcgcataaa gacgggcttc ggcctcttcg ggagagagcg accccggcag tcccgtctcc
     9781 ctggccagca cgtgacctct ttcatagatt tttttgcttt cgtggatagt cgcctcagcg
     9841 gctatcatct ctccctggac agtgttctcc gatgcgataa gcctcaggat catcctggca
     9901 tcggattcga tgcagcaatc cctgatcttc ctggttgatt ccggaccgat ccatatcctt
     9961 acctcttccg catcgacacc gtaacgtatg gatatggagc tccgcgcctc cctgaggaga
    10021 ttttccatta tcgcctcggg gcttctgtct gttcctgcag aagagggaag tatcacagcc
    10081 tgggctatca tctcacccgc aaataccact atccttccgc cccggatgta ttccagccct
    10141 tccctcagcc tctgggattc cgttttgagg gtgtcaactt ctttctccag ggacgatctt
    10201 tcattttgca gttcctccag gctggaaagg gtcgcctgtt gttcggcctt cgccctttcc
    10261 agttccgcct ctatctctct caaccgggtc tcgctgcgat ccaggtcacc ttcgacattg
    10321 gccaactgga gctgcttgga tacaaggtcc ttctggttgt caaggagtct agtctcaaga
    10381 tcaacgagtt ccgtcctgct cccctgaagt tgcgatgtaa gttccgttat ctgtttttgg
    10441 acgaatttca tgctgaagat agctgtccgt actgtctggg acgttgctga aagaacagcg
    10501 agtgtcagga tagttatcag tatccctgat actaccgtta tcagtgagga tgtggccctc
    10561 ggcctcagac caaagatgct gatccgcttc ttgccaaagc gcatgccaac aatgtcccct
    10621 atataggcga ctatggagct cactgtaaga aggataataa tcagcatcca gttcatttcc
    10681 atgaccagat ccatcaatcg accggcaccc cctgccatgt aacgttacca cattacacgc
    10741 ctctttgaac agcaagggcc ggggaaattc cccggccctg atattatccc gttttgtccc
    10801 tccgtgtggg aagattactt tgcgcactcc ctgaaaactt ctttggaaat gtccagggca
    10861 tgctgcaggt cttccagggg aacgttgagt gccggacgga accggatcga gacatctcca
    10921 cagggaagaa ccagcatctt tttggcccag aaggatttga gcgccttgtt gcgcatctct
    10981 ccactgtcaa agtcgtaggc acacatcaga cccttgcccc tgacgttctt gataagcgtc
    11041 gggaactccg cctggagttt cctgagtccg tccagaagcg ctggcccggc cgtattcgcc
    11101 acgtagtcca gcacattgtc ctcttcgtag atctcaaggt atcttgtcgc ccttatcatg
    11161 tcgaccaggg tcccgcccca tgtcgagttg atacggctgg aaaccttgaa acagttgttt
    11221 tcaacttcat caaccctggg gccagccacc agaccgcaga tctgggcctt tttcccaaag
    11281 gcgaacatgt caggttttac gggtgcgtgg tgttcgaagg cccacatctt gcccgttata
    11341 cccattccac actggacctc gtcgaaaata agaaggatct cttcctgatc acagatctcc
    11401 ctcaactgct ggaagaactc ggtccggaag tggttgtctc cgccttcacc ctggatggtc
    11461 tctatgataa tggcacagat atcgtccggg ttgtcccaga tagcctgctt gatctgcttg
    11521 acagctgtcc tttccagcca ggtcacaaca ccgtggttct cttcgagggg ccagtatatc
    11581 ttgggattga tgaccctggg ccagtcgtat tgcgcgaaga actggtactt gttgggatcc
    11641 tgggtgtttg ttacggtgag ggtataaccg ctgcggccat ggaaagcctc gttgaaatga
    11701 ataacctttg ttcccctgcg tcccttgatc gcatctccct tgctgatctt gcccttttcg
    11761 agaagtttct ggactttcca gtccattgcg accttgaagg tattttcgac tgcaagtgtc
    11821 ccgtagtcaa taaagaagac gtgtttgaat ccctcaggaa cggcaatatc accaaatgtt
    11881 tttacgaatt ttgccatagg ttcggtatag atgtccgaac ttgcgacctt gttgatggcg
    11941 gccttgaata ttgtctcctt gaattcgggg ttgtccagtt tggggtggtt cattccgaac
    12001 ggggcagaag caaagaatgt gtaaaaatcc agccagtcct caccgttgat cttgttgatc
    12061 atgtgagagc cctttgattt ctccatatcg atgacgatat cgaatccgtc gcggagaagc
    12121 catttcctga tctcctcaaa agcttccggt gcagcaacag tcctgtctac ctttgccatt
    12181 cgagctacct ccctgtgatt tattcttcgg gatgccaggc atcccgtgat tcctgaaaat
    12241 aaatttaccc gcgaccacca tactcatcct gactcgtccg ggctatctcc ggagggctca
    12301 aaccgggcaa gcggaacgtg gatcctaaag gtccgggtag ggacttttca gcaaaagaga
    12361 ctctatctcc tccgcgctga aaaattttcc gttattcatt ccagggcaat cagccgatgc
    12421 cctgtcccag tccgacctcg atcttaaaag gctcggccag aacgggaaca gagagcactg
    12481 tggtggccgg acaggataga tcatgcacct tgcggtcagg gaagaataca tgacgcattc
    12541 cccattcggc ttttccctga ggctcggcct tttccatctt cccgtaagga aagacctcct
    12601 gaaatcaccc tcgttcttct caaggtacct cgcagctgct gtaatatctt cgtcggaaac
    12661 ccatatcgca ccgggatcac ccctgcagca gcgaccacac cccaggcacg aaaaatataa
    12721 accctttgac caccatggtt tatattctaa accctcaccg gtcatttctt caaccttaaa
    12781 ttaccataac agaagttttt gcccgttacc atactctctt gtcccggcgt cttgaagtcc
    12841 ctatcagtga accactgttc ctgagtttat caatatcttc cagtgctttc cctgtcccaa
    12901 gagccacgca ctcgagagga gaatctgcaa cccaggccgg gataagggtt cttctcgtta
    12961 taagttcggg aagtcccttc agaagggctc cccctccggt aagggttatt cccctgtcga
    13021 ttatatctgc ggatagttcc gggggagtct gctcaaggac atgtcttatc ccctcgatta
    13081 tcattccaat ggtttcctcg atggatttcg cgattatccg gtcattgatc tctatctgcc
    13141 tcggcaggcc atgaacaaga tcccgcccct tgatgaccat tacttcttct gcctgcccag
    13201 gacaactgct tcctatcgtc attttgatgt tctccgctgt ctgttcccct atagcaaggt
    13261 tgaactcctt tcggacaaac ctgaggatgt tctcgtcaaa cctgtcccct ccaacccgga
    13321 gggattcggt taccactatt cctccgtagg aaagaacggc tatatccgtt gtcccgcctc
    13381 cgatatcaac aaccatgttg ccccttggtt cggaaatatc cagattggca ccgatcgccg
    13441 cggccatggg ctcctcgatc aggaaagcct ccttggctcc gatctcgaca gccgcttcca
    13501 gcacggctct tctttccaca tcagtcgcgc ccgatgggac acatatcatt actctccggc
    13561 gggtgagacg ggcaaagccg gtaattactt tcttcaggaa aaatctgagc atggcctctg
    13621 tcattgtgta atcggcaata acaccatccc tgagagggcg gatcgccaca acgttgccag
    13681 gggttcgtcc cagcatgttc ttggcctcgt tgcccaccgc cacgatcctg tctgtattct
    13741 ggtcaaccgc cacgacagag ggttctctca gaacgacacc ttttcccttc atgaaaatta
    13801 ttacgttagc cgttccgaga tctatcccga tatccgtgcc ccacatgcaa aattccctcc
    13861 ttcgatctat aacccgacga gaggcggcgg gagttttaag ccttttccat gatggaagcc
    13921 gccctcaagg cggcacccat agactatatc ctattcaggg ctggaccctc aagagcgatc
    13981 cagagaggtg tcgccataac cgactttcca aaggaagaga ccttttgccg gagcagtggg
    14041 tcctgcctcc tctctgctct ttccctggag aagagattca agccaggaaa tatctctttt
    14101 tcctgttcca accagatcga cagaacccag tatgatcctc accatattgg tcaaaaagct
    14161 ttttcctctt acccttacgc ggcaaagagg gcctctggcc gaaaaatcaa ctttggtgac
    14221 ggtcctcaca ctgttctccg gacggtcacc cagcctgcag aaagcaccga aatcatgttc
    14281 tcccttgata gcggaacagg cttcccttat tctcccataa tcccagaaag atgtattttt
    14341 ccatacatag ggttttatgt gaggtgggca cccgtgttcc ttccatacaa aatagatata
    14401 ttcccgccac aacgcgctgt ggcgagcgct gaaattatcc ggaacttcca gaacacgcaa
    14461 caccgtgacg ctttcgggaa ggtttgcgtc cagagcattc ctgagttttc caggctccca
    14521 atatctggac atggaaaagg atgctacctg cccggcagcg tgaactccct tgtctgtccg
    14581 ccctgccaca gtaacaggaa catttcctcc gtccaggatc gccagggctt tttcaaaagt
    14641 atcctgaatc gtcgctcccg ctatatcctt ctgcttctgc caaccgcaga actgcgaccc
    14701 gtcataggag agcttcgcgg catatttcaa cgaagatact gtcccctctc tgcaagaaat
    14761 gcggccacgg agaaaagaac cgctacagac agagccatgg aatccatacc gtcccagtca
    14821 aggggattca ttcttgtccg accttctcca cccgagtagt ttcttgcctc cattgcagtt
    14881 gcaagatcat cggctctctg aaaaacaatc acgaaaaggg ggacaagaac cgggataaag
    14941 gccctgagcc ttttaagtgg ccctcccctg tcaaggtcgg caccacgagc agtctgggcc
    15001 ttcatgatcc tgtccgtctc gttaagaagg gtcggtatga aacggagggc gatggtcatc
    15061 atcatggcca gttcgtgggc cgggaaaccg aatcttttaa agggtgaaaa gaggctttca
    15121 agcccgtcag ccagttctgt cgggctcgtt gtgatagtga gcattccggc gaacatgacc
    15181 agaagcagca gtctcatgcc cattttaaat gccattgcgg ctccttcacg ggttatcgtt
    15241 atgattcccc acgaaagaag aggttctccc ctggtaaaaa aaaggtgtat cacagtagta
    15301 aatactacca ggatccatac cggccttatc gttgatgcca ccaggcgggg cggaattttc
    15361 gcaaggatcg tcagtacagc aaaaaaagcg atccatccga cgaaaatcat cggaagaccg
    15421 gcgagaaaaa cccccgtaag gcaaaaaagg acagaaacga tcttgcacct tgggtcaagt
    15481 gaatgaacag cagaatcgac gggaacatat tgaccaaggg tcatgttttc gagaaatttc
    15541 atttttttac ccccgcgcaa atagcccggg caagcctttc cggctcccat gtcaggggaa
    15601 catccaggcc gacctctctg agccttgaag caaacctgac tacgggaggg accaccagtc
    15661 cgctgatatc tctctcggca agttcctcca gaatgaccgc cggagaaccc cacagaacgc
    15721 ttttcccctt gtccagaaca agcacgatat ccgcaagcct gagggccagg tcaaggtcgt
    15781 gggtgatatg tactataccc tttcccagcg acctgagatc tgaaagaaga ttgacaagat
    15841 ctctctttcc gccaaggtcg agtcccgctg tgggttcgtc aagaaccagg tagtccggct
    15901 ccgatgccaa taccgaggca atggccacac gccttttctg tccccccgag agcctgaagg
    15961 ggctgctttc gaagagttcc ggccctatgc cgacaatgga aagtgatctt tttacgttcc
    16021 gtcttgtctc atcctctgaa aaaccccagt tggccgggcc aaatgaaact tccctgaaaa
    16081 cggtctcctc gaaaagttgc tgctcggggt actggaagac cagacccact ttctgccgga
    16141 tactgcgaat agatgaatct ccatggtcaa tggttatccc gtctacagtc actgatccct
    16201 ctgaagacct caaaagaccg ttcaggtgct gggccagagt tgattttccg cttccggtat
    16261 gtccaacgat cgcgacccac gcccctgatg gcgcctcgaa ggtgaccctg tccagggccc
    16321 ttgtctcgat cggtgttcct gggtgataga tatgaacgag atcccttacc tctatggaca
    16381 taattcctcc accatggcct ctatttccgt cggagtatcc gctttcagcg ctcctccttc
    16441 gacaagcatt tcccactccc tgatgattcc cggaacttcc aggccccagg tctgaagccg
    16501 cttaccctgt ctgaatagtt cccttggggt accctgccag acattacggc cgccggagag
    16561 aactatgcat ctgtcgcact caaggatctc ttccagcctg tgggttatcg aaacaagggt
    16621 catccctccg gagtgaaggg cggaaaggat cttcatcagg tcatccctcc cccggggatc
    16681 cagcatagta gtcgcctcat ccattaccag gcaggggggt ttctgtgcga tggcaccggc
    16741 aacggcaagc ctttgtttct gtccgccgga aagggcatag acaggcttcc gggccttgtc
    16801 caccagtccg gatatcttca gggcccaacg gaccctcttc ctgatctcct ctgagggaag
    16861 acccatgttt tcggggccaa aagccacatc ctcttcgact acagccgcaa ttatctggtt
    16921 ctccggattc tggaatacca tggagaccga tcttctggct tcttttcttc cttcatcgtt
    16981 tcttgtgttc aggccattaa caaaacaatc gccctgcgat ggcaccagca gggcgtttaa
    17041 atgtttggcc agcgtcgatt tgcctgaacc gttgctcccc agaaccgcta tccactctcc
    17101 gcgctttatt tcaaggtcga tactggatat cgcctggttt tccgaacctg gataggtgta
    17161 agaaacaccg gtgaaactag ccagacaatc gcctgtcatg acctctctac tagttcgata
    17221 atagccatcg gggccgcatc gccatgacgg taaccggtct tgattatcct ggtgtacccg
    17281 ccctggcgct ccccgaacct tcccgcaata tctccaaaga gcttctgcaa agccttgacg
    17341 ttccccatgc gggacgcgac cagtctcctg ttgtgcagat cacctttctt ggccttggtt
    17401 ataagtctct cggctacacg cctgagttcc ttggccctgg taagggtagt ttctacctgc
    17461 tcctccagaa aaaggctcgc agtaaggttg gaaagcatag ccatcctgtg gctcccgtat
    17521 cggccaagtt tccttctgtc aacacgatgc ctcatcgggc ttcatcctcc ttcaaatcgt
    17581 cctcatcctc tggttcctct cccttgatgc tcagcccgaa gggatccagt ttctcctcga
    17641 tctccctaag cgagatcttt ccgaggttcc ttatcttcaa aagttcagcc ttgtttcttc
    17701 cgataaggtc tcctatagtc tgaattccac ccctgagaag gcagttttcg ctcctgatcg
    17761 aaagttccag atccttgatc gggcgtttca aagccccgtc atcggtcaca ggttctggat
    17821 caggctgctg gatctgccct gcccagcctc cctgttcatc actaccgaga ccctgtacaa
    17881 gcatggcaaa aaagtgctgc aggatccctg aggcctctgt ggcagcctct tccggtgaaa
    17941 cagacccgtt agtccatatc tcgagaacca gcctttcgta gtcggttctc tgccctaccc
    18001 gagcatcctg gatctcgtat ttgacccttt ttaccggaga aaagaccgcg tctaccacaa
    18061 tagcgtcaag gggaatatca tcgggtctct ctctttcgga ggtcacgtac cctgtcccgg
    18121 attcgacaaa aagatccatc tccagggtgg ctccgtcttc aagggtacat atatacatct
    18181 cgggatccac gaattcaata tcactgtcag gtattatccc ttgcggcgta acggcacccg
    18241 ggccggtggc ctcgagtctt aaaacccttg gttctccgga atgggatatt attgcaagat
    18301 ttttaaggtt gagaaggagc tcaatgacat cctctctcat ccctggtatt gtgctgaatt
    18361 cgtggagaat cccctcaatc cttacggctg tcacggcggc accccttatg gaagacagga
    18421 gaacccttct cagcgcattc ccaagagtaa ctccgtaacc cttatcaagg ggacccagaa
    18481 cgatctttcc gctaaggggg gtgctttcct cgactctgat atcaggtctt atctgttcca
    18541 atttatttcc ccccagacgt accctatcgg gcatagaact cgactacgag ctgttccttg
    18601 acaggaactt ctatctgttc gcgcgacgga agggagacaa tatgtccttc ctgcttttcg
    18661 gcgttgaact caagccagcc gggcacgctc ctggaagcgg caacttccag gttctccttg
    18721 agaacggcgc tatccctgct tcccgagtcc acggatataa catccccggc ctttacaagg
    18781 taactgggga tatcaacctt ctgtccgtta acaagaaggt gtccgtgtct tacgaactga
    18841 cgtgcctggc tcctgctgac accaaaacca agcctatata cgacgctgtc gaggcgcctt
    18901 tccagtatct ggagaaagtt atgaccagtc tggccagcca tcttgaccgc agaagcataa
    18961 aatcttctga actgggtctc attgatgccg tagaaacgtc tcagtttctg tttctcccga
    19021 agccggatac catattcgct ctccttgccc cttctccggc tctgcccatg ctgcccgggc
    19081 ttcgaatttc ttttgttgat agcgcatttt tccgtgtagc acctgtcacc cttgaggaac
    19141 agtttcgtac cctcagccct gcagagccgg cacacggcac ctgtatatct actcattatc
    19201 tggataaacc tccttattcg gcccggttag actcttctgc gcttgggggg cctgcatccg
    19261 ttgtggggga ccggcgtttc atccttgatc gcatttacct gaaggcccgc ggcctgcaaa
    19321 gagcgtattg ccgattctct cccgggacca ggtcccctga cgatcacgtc tatctccagc
    19381 atcccgtggt cctgtacctg cttcgcgacc tgctgagccg ccatctgggc agcaaaaggg
    19441 gtcgacttcc ttgctccctt gaaacctaca ttgccgccag aagcccagga aagcaagttg
    19501 ccctgcctgt cggtgacact gataatggtg ttgttgaaag tcgagaaaat gtgggccaca
    19561 ccataactga tgtttttctt ttccttcctc ttgcctcttc gctggattct ctttgccacg
    19621 cgattattcc ctccctcgcc ttatgccaga gccctttaca gggccattat ttcttggctg
    19681 cgggcttttt cttgcccgcg accgcgcgac gaggaccttt ccttgtcctc gcattggttt
    19741 ttgtcttctg tcctctcacg gggagaccaa acttgtgcct cattcccctg taacacccga
    19801 tctcgatgag tcttttgata ttcatcgtga tctcgcgcct gagatctcct tcgaccttgc
    19861 agctttcttc gatctcggcc ctcagtttct gggcctcctc ctcggtcagg tccttgacgc
    19921 gagtatccgg gtcgatcgag gtggccttga gtatcttttt cgccgtcgtg ggaccgatcc
    19981 cgaaaatata tgtcaaggcg atcacaatgc gcttttcgcg cggaatatcc actcctgcga
    20041 tacgtgccat gttatgctac ctcctcgctc cctgtctctg tttgtgcctt gggttcttgc
    20101 tgcaaattat ccttacgata ccgttgcgct tgattattct gcagtgttcg cagacttttt
    20161 ttaccgatgg cttgactttc atctgaatcc acgctcctca aagaaaatct tttcgagcat
    20221 aacccctgct tcgacttttt taaaacccgt atcatttata tctatacacg atcctggcac
    20281 gtgtcaagtc atagggtgat atctggacaa gcaccctgtc cccgggcaaa atcctaataa
    20341 aatgcatcct catcttgcca gatacatgac ccagaacctt gtgtccattt tccagttcta
    20401 tcctgaacat cgcgttcggc aatggctcca atacttttcc cttgacctcg atcatgtcat
    20461 ctttcggcat tcggctcctt caccctccct gtcgcaattc ggagtatcca cccctgcttt
    20521 ttcgatcgca cagtcgctcc tgcctgtagg atcccggcca tgatccgcga tccgccgccg
    20581 gcaggtttcc ggcggaacgc ctcttactgc cagggcgtta ttacctccgg atctccaccg
    20641 gtaacgacta tggtcttctc gaaatgggca gcgtcggagc catcagccgt aactacagtc
    20701 catttatcct ccagagtacg gaccctctca ctgccactca ttatcatcgg ttcaatggca
    20761 agggtcatcc cgctcttgag ggtaacaccc cttcccgggg acccgtaatt ggggacctgg
    20821 ggtggttcat gaaggtttct tccgatgccg tgtcctgcat aatcccgaac tattccatat
    20881 cccctgggta ttacaaagga ctcaaccgag aagcctatat cccccagggt ggcaccttca
    20941 cgggcagcct ggatcgccct ctggagggat tcccttgtaa catcgaccag atcagccctc
    21001 tcgggggata tatcacctac aggataggtg taggccgcat cgccgtaata accctcaatg
    21061 ctcacgccaa catcaacgct tacgatatct ccctcttcaa gtcgcctctc cctggcaggg
    21121 ataccatgga cgatctcctc gttgacggaa acgcaaagcg tcccggggaa gggccttggt
    21181 atccccggga ccctgtatcc cttgaaagcc ggtttgcctc ctgatgaaag gatatactcc
    21241 tcggctctct cgtcgagagt caacgtatcc agaccgggcc tgacaatatc accgatcatc
    21301 atcaggacat ctgccactat ctttccagcc ttgcgtagat gaaccaggtc cgtctcgttc
    21361 tttaaagaga tcattacctc ttaccgactg ccttttctat gtctttgact acacgatccc
    21421 cgtcctgact cgcgtcaaca cgaaccagat tgcctttggt ctcgtaataa tctatcaggg
    21481 gcgccgtctg ctcttcatag acttcgagcc ttcgatgaac gacttcttcc ctgtcgtcat
    21541 cccgctgctg gagcacgcct ccgcacctgt cgcaaacgcc gggcactttg gtgtccttga
    21601 atcttatgtt atagatctcg ccacactggc cgcatattct cctgccggta agacgctgta
    21661 caacaagttc ttcgggaacc tcgaagagga tcactccgtc aagttccagc cccatccggc
    21721 cgaggagatg gtcaagagcc tccgcctggg gaacggttct tggaaaaccg tcaaggatga
    21781 acccctttgc gcaatcaggt cgagccaacc tgtcttccat catgtcgatg ataagatcat
    21841 cgggaacaag ttttccggag tccatatact ccctggcctt gttccccagg ggagtggagt
    21901 tttttacgtt ttcccgcaag atgtccccgg tggaaatatg cgctatatcc tgcctttccc
    21961 tgagaacagc agcctgcgtt ccttttcccg ctccgggtga cccaagaaat atcagtttca
    22021 tcacaaattg tcccttctag aacttgagca accccgaggt tttgcctttg gatctcttga
    22081 ggatcccctc gtagtgtctc atcagcaatt gcgcctctat ctggtggacc gtatcaaggg
    22141 ccacccctac cacgatcagg accgcggtgc ctccgaaata aaaggttgta atacccatca
    22201 tacgggtcat caatgtcgga agcagcgcca ccccagccag aaagacagcc ccgccaaggg
    22261 ttatcctgga catgatcttc tcgatgtaat cagcagtagg ttttcctggc ctgattccca
    22321 gaacgaatcc accatatttc ttcatgttat ttgctgtatc ggcagggtca aagaccacag
    22381 cggtataaaa atacgagaag aagatgatca gaagcacata gagagccatg tagacaaaac
    22441 tgtcaggcgc aagcaggcgc tgtataactc ccgatatatc accagaaaag aacctgacga
    22501 tagtggacgg gaaaagcagt accgatgacg cgaaaatgat cgggataacg ccggcctggt
    22561 taacttttaa ggggataaag gtgctctgac ctccatagac cttgttaccc acaactcttt
    22621 ttgcgtactg caccggcaat ctgcgctgac cctcctgtag aagcacgcag ccagctataa
    22681 cgattatcat cagcacaacc gcaagagtga ttaccagaac gctcatctct cccatggtca
    22741 taagagacca ggtcctgacg atcgcttccg gaagcctcgc gactataccc gcgaagatca
    22801 aaagtgatat tccgttgcca atgccatgat cgctgatctc ttcgcccagc cacattacca
    22861 ccagcgaacc ggcagttacg gtcacaatag cggctatgaa ccccagggcc ccaccggtaa
    22921 aaatacccag gttgccaagc cagaaagtca ttcctacagc ctggacaagg gcaaagaaca
    22981 cagtgcctat acgggtgtac tggactatct ttttcctccc ctgctcccct tccttctgaa
    23041 gtttctcaag ggcagggaat acaaccacca ggagctgcat cactatgctg gcgttgatgt
    23101 agggagcaac accaagggcg aatacgctga accttctcag cgcgcctccg gcgaagagat
    23161 caagaaaccc gagtatcccg ccctgctcga agagctgcga catagcctcg gcatccactc
    23221 cgggagtcgg gatatgagtc ccgagacgga aaaggaaaag tactcccaga gtgaaaagaa
    23281 tcttgcgctt gagatccggc agtctgaaag catcccgaaa ggcgtcaagc acctagacca
    23341 cctctatctt cccgcccgcg gcttcgatcc tggctgcagc ctgtttgctg aaggcgtttg
    23401 cctttacggt aatcgccctg gaaatttctc ccttgccaag aaccttgacg ggcaggtggt
    23461 cgttgctgat cagccccgcc agaagcattt cgggtatccc gacctcgctc ccggcctcga
    23521 aagcatcttc gatccgttca atattgacca cctggaagct ttttttgaaa cgcgcgttgc
    23581 tgaagccgcg ttttggggtc cttctggaaa ggggcatctg ccccccctca aaaccggggc
    23641 gtactccacc gccgctgcgg gccttctgtc ccttgtgacc cttgcctgca gtttttcctg
    23701 ttccgcttcc cgcgccctgt cccagccttt tgggctttct ctttgacccg ggagcaggcc
    23761 tcagttcatg taattccatg agcctctccc cctattccgc aacattttcc cagacgacga
    23821 gatgagagac ggtgtcaatc atccctctta tctggggagt gtcttcatgc gtcactgtgt
    23881 gccccatttt cctgaggcca agtgccacga ccgtcctggc ctgccttgga gagcggccta
    23941 tggtgctttt tacaagcttg atactgacct tggccatgac cctggccccc ctagacctgc
    24001 tgagcctgac ggatatcctt gccccggaga cgcattacct cttcgggggt acggagaccc
    24061 ttcagtgcct gcatcgtagc ataagctacg ttaacagggt tggatgttct tccgatgacc
    24121 tttgtgagaa catcgcttac ccctcccagc tccatgatcg cgcggactac agcgccggca
    24181 atgacgcccg taccgggagc agccggtttt agaagaacct cggcagctcc gaaatgcccg
    24241 attatgtcat ggggaatgct tctgccgacc ttcttgagtt cgacctggtt tttctttgcc
    24301 ttgtcgatac ccttccggat agcttcggag atctcccgtg ccttcccgat gccgatcccg
    24361 acattgtgcg cttcgtcgcc tactacaacc aggacgctga acttgaacct tttcccgccc
    24421 ttgacgactt tgctgaccct gtttatcgat acaactcttt cagccatttc ctgccctttt
    24481 gggccgccgt tattgcccct catatcctgc accctcctta cagcttcagg ccggcttcgc
    24541 gggccgcatc ggcaagtgcc tttacgttgc cgtgatacat atgaccgccg cggtcgaaaa
    24601 ggacctgtgt tatgcccttc tcgatggccc tctgggcgat cagtttgccg acgctctttg
    24661 ccgcttcaaa atccccgtca ccattggagt tcgacctgat atccttctcc agggtcgacg
    24721 cggcggctat ggtgtgcccc ctggtatcat cgatcaactg ggcatacata tgattcaggc
    24781 tcctgaacac cgaaaggcga gggcgctcgc cggtaccgga aagggtccgg cgaagacggc
    24841 gatggcgaat cagtcgcata tcgttacgtg tccgtttttt catcctgccc atcacctcac
    24901 tgactaggct ccggccttgc cggcttttct cgaaacactt tcgccagcat accttatacc
    24961 cttgccctta tatggctcgg gtggcctgaa acccctgatt atcgccgctg tgtgacccac
    25021 ggcctgttta tcaattcctg taaccgtaat ttttgtcggt ccgtccgttg atatctcgat
    25081 cccgctgggg ggttcaaatt cgaccgggtg ggagaaaccc aggttcatga ccagagtctt
    25141 tccctgcatc tgggcacgcc atcccacacc tacgatctcc agacgccttt caaaaccctg
    25201 gctgacaccg gtgatcatgt tgttcaccaa cgccctgacc atcccgtgaa aggccctggt
    25261 ctttttttcg tcgttctgac gggttacctt taccgaacca tcctctacag ccacgtcaat
    25321 acccgcgacc agatctaccg aaagttcgcc tttcggacct ttgaccttga ccttgctttc
    25381 ctccactgcc agggtgaccc cggccggcat gccgatcagt tttctgccaa ttctagacaa
    25441 ccaggacacc ccctaccata cataacagac gacttcgcca ccgagaccgt gggtccgggc
    25501 ttcggaatcg ggcatcatcc cttttgatgt agagatgatc gcggttccaa gtcccttcaa
    25561 tacactcgga agttcgtcct tctgaacata gatccttcgc cccggtttgc tgatcctcct
    25621 gagtccctgt atgactcttt ccctctgcgg cccgtaggtc aggaacagac ggagcgtagc
    25681 ataaggtttc ttgggatcat tgacgacctt gaaattcctg acgtacccct cttctttgag
    25741 tatcttcgca atagcaagtt tcactttgct aaggggcata tcaacagttt catggtagac
    25801 catgttagcg ttccggatgc gcgtgagcat gtcccctaca gggtcattca catacatcgc
    25861 aatagcctcc ttgatcgcat cccgtgctac cagctggact tgacgacacc gggaatttta
    25921 ccttcccggg ccagcttccg gaagcaacag cgacacatat taaattgcct catgtaggcg
    25981 tgcactcgac cgcagatagg acacctggtg taggctctca ccttgaactt gggttccctg
    26041 gcggccttta ttctcatagc ttttcgggcc attcgctaca tctcctccct taccggttga
    26101 aaggcattcc cagagcctga agcaggctca tcgcctcttc atccgtcctg gcggtagata
    26161 ctatggaaat attcataccc ctgagccggc tgaccttgtc gtattcgatc tcggggaata
    26221 ttaactgttc cctgaggccg aggttgtaat tcccacgacc atcgaatcct ttcgcggaca
    26281 gtccctggaa atccttgatc ctcggcaggg cgaccgaaat aagcctgtcg agaaattccc
    26341 acattctgga acctctcagt gtcacggaac acgctaccgg cattccctct cgaagcttga
    26401 aacccgcaac ggatttcttc gcccgcttca tcatgggctg ctgacccgtt atggtccgaa
    26461 gttcagatat cgaggcctcc atatacttgt agtcaacctt ggcctctcca actccgatat
    26521 tgacaacgac cttctgcacc ctcggaacct gcataatgtt ggaatatcca aactgcttct
    26581 gcaggcgtcc ggttatctcg tttttgaaca tatctgccag acgcggcata atcccgtcgc
    26641 ctccccgtta caccttatct attatttcgc cgcacttttt gcagacgcga accttgcgac
    26701 cgttctcaag aaaagcacgc ccaaccctgg tcgccgcacc acacgcagtg catacgagct
    26761 tgaccttgct ggagtaaatg ggagcctcct gcttgactat tccgctctga ggattctttg
    26821 ctgagggctt tacgtgtttg gtgacaatat tgacaccctc gacaagaacc atttccctga
    26881 ctggaaaatg cctcagcacc ttgccttcct ttcccttgtc cttgccagag atgacgtgca
    26941 ccctgtcacc cttgcgaata cgcattttgc tcattgcccg agtcctccta cactacttcc
    27001 ggtgccaggg atacgatcct catgaaccgt ttctccctga gctcacgtgc cacggggcca
    27061 aaaatacggg ttcccctcgg gtctccgttg ttgtcgataa gaacggccgc gttgtcatca
    27121 aacctgacgt aggatccgtc acgcctccga atttcctttt tggtccttac tatgactgcc
    27181 tttacaacat cgccctttcc agcgttactg ttgggtatgg cttccttgac ggaagcgacc
    27241 actatgtctc cgacgctggc gtatcttttt ttgcttcccc cgagcacctg gatgcacatt
    27301 attttttttg cgccggaatt atcggcgacc ttcatctttg tccggagctg gatcattcgt
    27361 tatgccccct tctcctggct accgccaaga accggcgcac gttcgatgat ctccacgacc
    27421 ttccagcgtt tgcacctgct gagaggccgt gtctcctcta tacggatcct gtccccgata
    27481 cggcagctct cttcctcgtc atgagccatg aatttcttgc tcctcagaac gggtttgccg
    27541 tagagagggt gcttggccat ccgatcgacc tttatgacca ccgtctttga catgcggtca
    27601 ctaacgactg tgccgactct tgtctttcgg gaaattacct tttcatccat tgcctttccc
    27661 tcctacgccg ggcgcccggg cccgatctcc ttttcgcgca tcaccgtcag gaccctggct
    27721 atggatctct tgacgtcacc tatacggccg gtattgctca actgaccgat ggcatgctgg
    27781 aaacggagat tgaaaagttc ttccttgaac attttgtgct tgtcggaaag ttcttctaag
    27841 gacaattcgc gcagttccct gctattcatc cttattcacc acccattcca tcacgggaga
    27901 caaggcggac cttgatcgga agcttgtggg aagccgtcag aaaagcctgc tctgctatct
    27961 cccttgacac tcctgatatt tcgaacatga tcctgcccct tttgacggga gcgacccaga
    28021 attccggatt tccctttccc tttcccatac gcgtttcaag gggttttttg gtaaaggggt
    28081 gatcagggaa tatcctgatc catgtttttc cgcccttttt catcttacgg gaaatcgcca
    28141 cacgagccgc ctctatctgt ctcgctgtta tccaggcgca ttccagggcc tggagaccat
    28201 actcaccgaa ggcaaccgtc tttccacctt tagccatccc gctcagggga cggcggaaag
    28261 gtttgcgata cttgaccctt tttggcatca gcatcttcgc ttacctcctc tcggccgtat
    28321 cctgctggat tccggctgga gcaggccgca ctgcctgacc cttgtatatc cagaccttga
    28381 tgccgattat tccgtagatg gtccttgctt cggcaaaacc gtagtcgata tcagctctca
    28441 gtgtcgagag gggaaggcgc ccctcaagat accattcggt cctggctatt tcagccccgc
    28501 cgagccttcc gctgcactgg atcttgatcc cctcggctcc agcctttgta gcccggaata
    28561 ttgactgttt cattgccctc cggaaactga cccttctttc aagagccgat gccactccct
    28621 cggcgacaac ctgtgcttcc ttgtcgggat tctttatctc ctggacattt atcatgacct
    28681 taccgccggt caattcctgg agttcttccc tgaccttctg gatttcggtc ccacccttgc
    28741 ctattacgac accaggccgg gcggtccata tagtaaaacg gataaccttg ccgatgcgtt
    28801 ctatctctac cttcgccact ccggcgttgg cccacctttt cattatccac ttccggagac
    28861 gcagatcttc atgcagtttg gcggcataat ctcttccgcc cgcataccat ctggactccc
    28921 agtcgcgaac tataccaagt ctatacccaa cggggtgtac tttctggccc aacggtcaac
    28981 cctccctagc gctcggcaac cacaacggtt atatgactgg tgcggtgctt gtagccgtgc
    29041 acccttccca tcgaaaccgg ctgccaccgc ttcattgatg gaccctggtc cgcatatgcc
    29101 ctgacaatga ccaggcggtc catgtccatt ccgtaattat gctccgcgtt tgcaatagcg
    29161 cttttcagga ccttttctac gaccctggcc gcatctttgg gggtaaaacg cagctcatcc
    29221 agagccttcc ccgctttttt gccgcggatc agattgagga cctgtctcga tttggtaggt
    29281 gacacgcgca cctgccttgc aactgccctg gcttccataa acagcccctc ctaacggacg
    29341 cgggtgtggc gttcctgtcc ggcgtgaccg ccgaacttgc gcgtgggcgc gaactcccca
    29401 agcttgtgcc ccaccatgtt ctcgctgata aagatcggaa tatgtgtcct gccgttatga
    29461 acggctatag tatgcccgat catgtccgga acaatagttg aacgcctgga ccacgtcttt
    29521 atgacctttt tcttgccctc ttcgttcatt gcttccactt ttctcagaag tttctgttcg
    29581 ataaagggtc ccttcttagt ggaacgagcc attaccgtcc ctccctgcca ctacttgtca
    29641 aaacggcgcc gtactatgtt cttgtccgac ggcttgcgtt ttctggttct gtaccccttt
    29701 gcgggtgtgc cccaggggga tacagggtgc ttgtgcgact tgcttcgccc ttcgccacca
    29761 ccgagtgggt ggtcaacagg gttcatgacc attccgcgga cgtggggacg acgccccagc
    29821 caacgagtct tgccagcctt gcccgatgtg aggttctcgt ggtcctcgtt tcctatctgg
    29881 ccaactgtag ccatgcattc gagcagggca agtctgagtt ctccgctggg gagccttatg
    29941 aaagcctttt tgccttcctt ggccatcaac tgtgccgacg ttcccgccga gcggaccatc
    30001 accccacccc tgccgggctc aagttcaata ttatggatga aggtacccac cggtatatcc
    30061 ctcagtttga gagcattgcc cggcctgatg tcggaatctg gacccgccac tatggtatcg
    30121 ccaacaccaa gtcccaccgg ggcaaggatg tatctcttct ccccgtcggc ataatgtatc
    30181 agcgcgattc gggcagagcg gttgggatcg tattcgattg ccgcaacctt gccgggaact
    30241 tcaagtttgt ccctcttgaa atctatgatc ctgtaaagcc tcttggcacc accgccgcga
    30301 tggcgcatgg taaccctgcc gagattattc cttcccgcgg tcttcttcag gctttcgaca
    30361 agggacttct ccggttcctt ttttgtgatc tcctcaaaag aggaggtcgt catggttctt
    30421 cgtccaggtg tgtatggttt atattttttg atacccatat ccgtatcctc cccgactacg
    30481 cgttagcgcc ttcaaagaac tcaatgcgct cgccggccgc gaccgttacg acagccttct
    30541 tccaggatct cgacttgccg aggaaggcgc ccattctttt gggctttggc ctcatgtgga
    30601 tggtgctgac ctttacaacc ttcaccttga agagttcgac aaccgcatcc ctgacctcta
    30661 tcttgttggc ccgggggtga acctcgaaag tatacttgtt ctgctccatc atgcggctgc
    30721 tcttctctgt aataacgggc cttatgatga tatcctgcgg catttccttc atgccccgta
    30781 cacctcctcg atctttcggg cagcatccag ggtaagaatg aggtgcctgt gattcaggat
    30841 gtcgtagacg ttaatgctat cgacatggag aagcatcaga cccggaatgt ttcccgcgga
    30901 cctggagacg gcaacgttgc tctcactcac cataaccaga ggtttcttgg acgcctcggc
    30961 cacgttgaga aaggacacca tcatcttcgt gctcggtttt tccatatcga tcctgtccag
    31021 aagtataagg cttccctcgc cggctttaag cgaaagggca cttctgatcg cgagccttct
    31081 gaccttgcga ttgactttct ggtggtaatc ccttgggtgc ggcccatgag tgataccgcc
    31141 gccgacccat atgggggatc tcctgctgcc gtgccgcgcc ctgcctgtgt gtttctgttt
    31201 ccagggtttc ttgccaccgc cccgtacgtc agcgcgagtc ttggtggaat gcgttccctg
    31261 tctcgcattg gcctgctgtg ccacaaccac ctgatgcatg gccggcacat ggaccggggc
    31321 cgcgaatacc gcttcggaaa ggttttcttc gcctatgacc tctccctgaa aattcattac
    31381 cttcacagtc ggcatggtat ccctgcctcc ttaccgtgct gccccgtcct gcttgcggat
    31441 cattacgagt ccgttcctcg gacccggaat ggcacctttg acaagcagaa ggttgttttc
    31501 gatatcaacg ccagcaatgg aaaggttttt taccgtaact ctttctccac ccatcctgcc
    31561 aggcattgtt ttgcctttaa aaatatgtcc aggataactg ctggctcctg ctgacccggg
    31621 ctggcggtga aacttggacg cgccatggct cccggggcca ccctggaaat gatgccgttt
    31681 gatgactcca gcaaaacctt ttcccttgct cttgccggta acatcaacgg tatctccctc
    31741 ctggaaaagg tctacagaaa caaccttgcc aggttcgtac tcccctgtgt cgtcaacgcg
    31801 gaactcgcgg atccatcttg ttggctcgag tccctttttt tcgaatacac ccctgtaagg
    31861 cctgggaagt ttctcggctt tgacctttcc gaaaccaagg ctgaccgagg tgtacccgtt
    31921 cttctccatt gtgcgaaccg caaccaccgt acagggacct gcctctataa cggtgaccgg
    31981 gacagaatgc cccttttcgt cgtagatctg ggtcatgccc agcttgcgac caagaattcc
    32041 catactcatt tgtcgcatct ctctcctttt cagctcagtg ctcccccact acagcttgat
    32101 ctgtatgtcg actccggagg ggagattcag ctgcataagc gactccatgg tcttttgcgt
    32161 agggtcgatg atatcaatga gcctcttatg cgtcctcatt tcaaactgtt ccctggcatc
    32221 cttgtccttg tgaggggact tgaggaccgt aaacttgttg atctccgtcg ggagggggat
    32281 aggcccggaa accttggccc ccgtgcgctg tgccgtctcc gctatctgaa acgcggagga
    32341 gtccagtgcc ctgtgatcga acgccttgag cttgatccgg atcttctttg ccactgcgac
    32401 ttcctcccta tgatcctagt tatccagaat ttcggtcacg acgccggcgc caacggtcct
    32461 gccgccctcg cgaaccgcga agc
//
LOCUS       DAOTIQ010000012        32287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1552_length_32287_cov_8.149169, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000012 DAOTIQ010000000
VERSION     DAOTIQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 32287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32287
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_32287_cov_8.149169"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1037)
                     /gene="fliG"
                     /locus_tag="P5541_03065"
     CDS             complement(30..1037)
                     /gene="fliG"
                     /locus_tag="P5541_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRV70987.1"
                     /translation="MAEMHEIKGRRKAAILLVLLGPEKASKVYKHLDEQMIEILTLEI
                     AAVRRIDPGQKELVLTEAQEIIMAREFLDRGGVSYARELLEQSMGEAKAEDLLRKLTS
                     NLQAKPFDFLRASDPIQILSFLQGEHPQMITMILSFLPPEKAAAILSGLPNQDQADVA
                     LRIAKMDRITPDVLRETEKELEKKFSAVVGGDYSIAGGIDSVVQIINRAGRKTEKNIL
                     EILDETDPELAEEIRKKLFTFEDIVKVDDRSLQKALRDIDNKELAMALKGVSDDMREK
                     FYRNMSSNAVEIIKEEMEYMGPVRLRDVEESQQKIVNMIRALEEAGEIVLGGSGDDIV
                     I"
     gene            complement(1040..2590)
                     /gene="fliF"
                     /locus_tag="P5541_03070"
     CDS             complement(1040..2590)
                     /gene="fliF"
                     /locus_tag="P5541_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRV70988.1"
                     /translation="MKNYKEILSGIRIFWASLSRARRFTAAGVTFLLLAFLGGVVFFA
                     GIKNLVPLYSGLELSDQAAIVEFLKENNVEYRLDPGASSILVPEERVYDLRLMLASEG
                     MPKGGTVGFEIFDKVKMGMTDFQQQVAYIRAVEGELARTIRQLDVVETARVSVVLHKP
                     RLFLKDEMPASASVLLKLKRGRDITREQVRAVMNLTASSVEGLEPSNVSVVDTSGRLL
                     SDVVEDSFFMLPGSTGGVSSMQRELEKMQERDLETKIQNMLSAILGPGNSIVRIRIEL
                     DFTRRNQTRQEYLPVPGGKGGILRSAHKTEETYTGASASTQAVGTAVNIPGYAVQSSG
                     GGSGEYGKTDDISNYEISSIQQQEEETPGSVKRITASVVINGEEGSLPSDQLLASLSS
                     AIGIDEGRGDRLTLSFIEFVPEASAAEVPADVAGQQGRGFAFPLWFLPVVIGCAVLVF
                     ASRAFIKRKRGVTEDKREKELSEEEMPDLFRTKKGGIQILEEQLGVYAENNPEDVAGI
                     IRQWLGES"
     gene            complement(2612..2956)
                     /gene="fliE"
                     /locus_tag="P5541_03075"
     CDS             complement(2612..2956)
                     /gene="fliE"
                     /locus_tag="P5541_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRV70989.1"
                     /translation="MRSSMINLSLIGNARMAYGSSVKPVENRFVNENKPVNGEKKVSG
                     FDDALRTGLEKVNDVQIRAEEAMKAMVTGDVEDISEVAAAVSEADMALRLAVQIRDKL
                     LDAYNQLVRISV"
     gene            complement(3000..3806)
                     /locus_tag="P5541_03080"
     CDS             complement(3000..3806)
                     /locus_tag="P5541_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HRV70990.1"
                     /translation="MEEHVSTSAQVRGQEESIWENYRLTLPRSPERKGIQEELVRKYQ
                     YLVRFIVRRMNVTPPRELDYEDLISYGKMGLLDAIDRFDPSLGFAFPTYAASRIRGAI
                     LDELRRFDWISRSGREKIHALDRALEEEMRDNGSVDHDRVRERLGLSPEQYQELTEIS
                     NRSFVGCLDETVALDDSEVGKADILCDPGPDQEELVIRKEEIEELHQALEMLTERERR
                     LVQLYYFNYRNFKEISREFGLSESRISQLHKRVLAKLQQNLVSARMEIAP"
     gene            complement(3962..5038)
                     /gene="flhB"
                     /locus_tag="P5541_03085"
     CDS             complement(3962..5038)
                     /gene="flhB"
                     /locus_tag="P5541_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRV70991.1"
                     /translation="MDLQIFSQEKTEEATPKKERKEREEGRVAHSRDLTSSVMLVTGT
                     LFLFAFSGLAWRIYRSFLTDTIGLLPSIGQGKGDWVNRLPGSGVLCFFRIWLPLSLVV
                     LLSALLVVSLQVGLKISPKPLIPKLDRFSLVKGLKKMFSVRSFVEAFKAVLKATILFA
                     VLFFGLKNEFIILYEASRLDVSVGIVFIFTLLFRLALRLALALFVLGLFDYIYQRWEH
                     SRSIRMSKQEIRDEFKQTEGDPLVRQRIRRKQQELGQHRMMSRVPEADVVVANPTHLA
                     IALKYERKKMGAPVVLAKGAGFVALKIREIAETNGIPVVEEKSLARGLYPRINIGEEI
                     PEEFYVAVAEVLAYVYSLEKKKPL"
     gene            complement(5148..>6858)
                     /locus_tag="P5541_03090"
     CDS             complement(5148..>6858)
                     /locus_tag="P5541_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRV70992.1"
                     /translation="VSVEPDISHLGLSSFLGLETHAVSTQAEISSVIGDTTASPLDMV
                     FSSGTKRLNLRIADDPALTLETFAARIRAAAGDWLEVVVESDQPEVSPTDSTSLNSET
                     GTLRLLLKAKDGGPLAIFDREGSYARSLGLDSALTTSDLSTVTFPGTAPPDVPLLIGV
                     EAGGEIYRVKLYKRDIALPDGTIDSQALAREIIGQTGDELIAAEAVQSGSSLVFYSAT
                     GEPVRFIDLPYSDPSMAGATSGFAMAAGLHAGVTGEPVDPTLAAGSESSFTICTGGRS
                     ITVSVGSSETIEDVASRVGELAGGWLDVSLASDGSGNMRLALAAKDGSPLNIYDVTGS
                     AAEDFGFNTDVRARAGTWTGGGTLRVEIDGYSLEIDLKEETTLEGVANHVNMRFAGGD
                     LKARVVNNGGTEELVFFSPRGNSFRIQAPAEMVLPAGDEAPSRSAGGPGGPMAQTVTV
                     RRGADVEEADIFAILDDLIFAVRQGDSEGLSNSFLPRLDQGIDDVLKARAYCGAIQRR
                     YDISGSRMRTNNIAMTDLHSSIMDVDLAEASIEFQTSQMIYQATLATISRVVQPTLVD
                     YLS"
     assembly_gap    6859..6958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6959..7857)
                     /gene="flgL"
                     /locus_tag="P5541_03095"
     CDS             complement(<6959..7857)
                     /gene="flgL"
                     /locus_tag="P5541_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRV70993.1"
                     /translation="MQRISQSMMSSVFLQDMHGSLNRLMQIQKRIASQKQYSKPSDNP
                     SEVARGMAVSTSLARNEQFQRNLDDAVSWLSNTDTALNQITDIVSAIREKVIYACDGA
                     LSRPDLDAIAKDIEALRDELVQTANFDVEGRYLLSGFDTASPAFSRNSDGTVTYQGND
                     GRIRFQIEKGETGLVSLNGRDVFPLGYTRHSVTSIELPMDFRWTGSSENLRISVGDRT
                     VQVPVPQRWIDNNRDSLSDNSDHDGFRSPGEPLNGYSLIEIAELLNDSAGAGSLVNAS
                     VETDSLSGIQRLVIRSLTGEPMQ"
     gene            complement(7914..9845)
                     /locus_tag="P5541_03100"
     CDS             complement(7914..9845)
                     /locus_tag="P5541_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HRV70994.1"
                     /translation="MLRSLMSGVSGVKGHQTLLDVVGDNISNANTAGFKKSSVNFQEL
                     MSQTEKSATAPGAEKGGSNPNQIGLGMSIGSVIVDHSQGNINYTGAKGDIAIEGDGFF
                     VVEQGSNKVYTRAGAFILDGNGDMVQSGTGSRLQGFTMSDDPLNPGNKIVGTSLNDIN
                     IPVGQKIPAKATTTAGFRCNLDSRVGTILPMGLSGNDVVFQGTIGNVQYDSITFSKGT
                     GLNDFIRATFTNAAGSQTTVNLPIAGINPTFGLPVLTPTTADLSGPASNDTISFDDTT
                     GQVSVVSSGGTVLWTGDLGAALDYHVVNISDGSGGTLQYLAHFDDIASGGRSLVLTGL
                     NDNATTPAMERVQLDLDADDDGTFIVPAGTTVTLNTGGVTPQTLDVDSTTLGMGIALS
                     QGGTSVKNFDLQTASLHSTKFNIFDGQGNSYTLETSWEKVDNNVWRWRAWLPDTPGVS
                     LGNNTGLLEFHPSGLVDYSSGPSTVTINFASLGAPNSQILLDLTGQAMGVSPTDSVTQ
                     FGSAFTTKEYYQDGYRMGVLQDYSVGSDGIIRGIYDNGQTQSLYTLAVAVFSNPSGLE
                     KMGNGAYRDTANSGIPQILKPMEGGAGSLAGKSLEASNVDLTSEFVNLIKAQRGFQAS
                     ARVITTSDQILEELMNLKR"
     gene            complement(9931..10347)
                     /locus_tag="P5541_03105"
     CDS             complement(9931..10347)
                     /locus_tag="P5541_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRV70995.1"
                     /translation="MSTSSVELTSGSLPGTGAAAEKAAGNSLLGKDAFLKILVTQMSN
                     QDPLEPLDSAQFIAQMAQFAEVEQTANLNDKLANLGEHIRFSAASLVGSVVTFADDDG
                     ADREGVVQSVLFEKNSISVKVDSGEEVSINRISRIG"
     gene            complement(10460..11914)
                     /locus_tag="P5541_03110"
     CDS             complement(10460..11914)
                     /locus_tag="P5541_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRV70996.1"
                     /translation="MTESRRPVCIHGHFYQPPRENPWSGEIDLQQSAAPYHDWNERVT
                     AECYEPNASARILGPDGGVLKRIDNYSLISFNFGPTLLSWMERERPELYTKIIESDNK
                     SRKRFSGHGGAIAQCYSHMIMPLASARDKKTQVLWGVSDFKHRFGRKPEGMWLPETAV
                     DTETLEIMADEGILFTILTPHQALEVRGHGGKWRDVSGGGIDTSVPYVCYLPSGREIA
                     IFFYDGAIAHEIAFGDLLRDGAVLASRLAGTGNAVGKSPGLSHAAVDGETFGHHHKFG
                     EMALAYCLESIEAGMEAQLTVYGEHLDRFPPQNEVRIAENTSWSCVHGVERWRSDCGC
                     TAGGHPEWNQKWRAPLREALDGLRKVLDPLFEEKGSGFFPDPWEARDGYMDRILSGES
                     KALPEEAHDLLEMQRFSMLMFTSCGWFFDDISRIETLQILRYAARAIEIAEKISGASI
                     EAPFIDVLEKVPGNTAEIKNGRIAYEKSKGANKG"
     gene            complement(12040..13974)
                     /gene="glgB"
                     /locus_tag="P5541_03115"
     CDS             complement(12040..13974)
                     /gene="glgB"
                     /locus_tag="P5541_03115"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRV70997.1"
                     /translation="MRYDTSLLTEQDIYLFREGRHCRLYERLGAHFTDLPEGEGTHFA
                     VWAPGAGEVSVIGDFNGWDRGSSPLKPRWDSSGIWEGFVPGAVHGARYKYFIKSSDGR
                     WSGEKADPLGRFMEPPPGTASVIWDDTHDWKDGQWMENRSGSSALNRPISVYEIHPGS
                     WRRGEDGEFLSWRQMAPLLVDYISGTGFTHVELLPVMEHPFYGSWGYQTTGYFAPTAR
                     YGTPSDLKYLVDVLHQAGIGVILDWVPSHFPDDPHGLARFDGSCLYEHSDPRQGFHPD
                     WKSCIFNYGRNEVRSFLLSSAAFWADRYHADGIRVDAVASMLYLDYSRKEGEWVPNIH
                     GGRENLEAMQFLRDLNVSLYRDFPGIQTIAEESTAWPMVTRPVHTGGLGFGLKWNMGW
                     MHDTLRYFSLDPVFRKFEHNLLTFSAWYAFSENFMLPLSHDEVVHGKGSLFARMPGDD
                     WQKFAGLRLLLGYMYAHPGKKLIFMGGEFAASREWDHESSLDWHLAGMGPNAGVTLWI
                     SDLNRIYRREPALHEKDCFHEGFSWLDARDRDSSIVSFLRFGGEEAVVAVCNFTPVPR
                     HGYVLGVPSGGRWEEMLNSDAECYGGSGQGNMGGVAATGEPSHGFPFSVALTIPPLGI
                     ILMKQEKGENGERRSLNGKR"
     gene            complement(14006..14389)
                     /locus_tag="P5541_03120"
     CDS             complement(14006..14389)
                     /locus_tag="P5541_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV70998.1"
                     /translation="MTARRNILTAVLVVAIAFSGTAFASVGEELVGNYRGTFIGDDYG
                     SFLIDVGPDGSITGTVHSNVSFLDMDVKGTCAPNGECEFVTEGEEAVPFYFMGRIDFM
                     NRFIGKWSYSDHSGRGSFYGMIQKE"
     gene            complement(14459..14986)
                     /locus_tag="P5541_03125"
     CDS             complement(14459..14986)
                     /locus_tag="P5541_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRV70999.1"
                     /translation="MNEERISEGIEEYLGRLKSRGFRLTNQRRVIVETLLRHLGTHLN
                     ARELLDLAQVEDPSIGIATIYRTIELLNRLGMLNMVNLEEGFLRFEVPDDRMHFHIFC
                     RSCGRTVHLSDEDGKMEEVLRWAKEEGFDLLPQTFEMAGLCMDCREGGILETEEFPPG
                     MGRRRCRRRFGGLKA"
     gene            complement(15008..16528)
                     /locus_tag="P5541_03130"
     CDS             complement(15008..16528)
                     /locus_tag="P5541_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRV71000.1"
                     /translation="MNKGRSFLAVFTVAAVLAAGGAASGAENSLTAGKVDVYPRGALL
                     SIEMEAEPLMQIELPLTLDPGNMTVTAGEGVTVQGYDIVEAARPGWVPPALASLADEL
                     DKARSRVDMLRARASALEQARKHLGEAVPENLKGSELEAFVDTAVKKRESVELKAAET
                     EILLEKAMKEHDLLRSEYESQFPGDPDRVLLLSLETEGKGKINLEAWTREAYWRPSYR
                     MGLNSSTGEVTVKLAADVSQKSGITWNGEILLHTVRPKGGINIPEMRPLVVDFLEISP
                     APKAMRGAMEEMAASVMMDEAVPQEPLMEEGLTDVTLKARGIVNGFGKKEMLELGKFS
                     EKSEVSLVAIPEFSSEAWTVSTVKSLGRSILPGEARLSVDGKETGVTNIPRRSMGQEL
                     QIPFGTTPLVTSRREEMLPEKGSSWIIRGKHQRGYVITVSNGLREKVTLKVMDRIPVP
                     ARDNIKLQDLVVSPEPAEKIEKGLVTWNVELEKGQSSEISVKYTVTYPADKELMFR"
     gene            16648..17739
                     /gene="ychF"
                     /locus_tag="P5541_03135"
     CDS             16648..17739
                     /gene="ychF"
                     /locus_tag="P5541_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRV71001.1"
                     /translation="MLNCGITGLPLSGKSTIFNVITRAGAEVKPYAGGKTDPNRAVVS
                     VPDDRFNRLVEIYQPKKITPAPVEFVDLAGLSRDASKGAGLGNSFLSFVAESEALIQV
                     IRCFDNPEVPHPEETVNPVRDWNIVETELILRDLSVIENRLGKLQEKKKPSPEEQAEK
                     ALLERCRDHLFEERPLREMGFSPDEARTVRGFTFLTLKPELVVLNMDETQASGTMPWM
                     SEMEKLAADRGLSLVRLFGRMEMEIADLEVSEQAEFMSELGISEPGRDRLIREAYSLL
                     GLMYFFTVGKEEVKAWTIREGSTAVEAAGAIHSDLARGFIRAQVVHYDDFEANSFSNA
                     QCREKGLLRLEGKDYTVRDGDIIEIRFNV"
     gene            17761..18768
                     /locus_tag="P5541_03140"
     CDS             17761..18768
                     /locus_tag="P5541_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRV71002.1"
                     /translation="MSILFEPLEIRGIRLKNRIISAPMASNTCTKTGLPTADTLDYYM
                     PLAESGVGLAIVEHHAVHPDGRARERQLMMDRDEIIPYQKDLSGLFSSRDIPAMVQLN
                     HAGSQIMDEELLVNGWAPKGPSPIRHPRSKVFIMPQGLTTEDIASLPGLFADSAKRAV
                     KSGYIGVEIHACHGYLLGQFLSPMTNLRTDAYGGEIRNRARLLFEIHEAVRDAVSDET
                     VVAVRLGLADTLPGKEPSGQTIEDSRWVAGEFSSRGLDLLDLSGNMCGYEGQGEAWFA
                     PYCRTVKDAAGSIPVVCTGGIRTADTAVRLLERGDCDITGIGRALKANPGLVHDWEKD
                     Q"
     gene            18765..19667
                     /locus_tag="P5541_03145"
     CDS             18765..19667
                     /locus_tag="P5541_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRV71003.1"
                     /translation="MTFLGIDLGGQFIKGALVDNGLIKAREKVPTPEGRDPDGVSIAI
                     SGILDRLDPDRDAESVGVGFPGMLDQDREKIFQAPNFPLLKNCHFRKYLQEKTTRRIF
                     LENDANCATLGEWRSGAAMGLTDFVMLTLGTGIGGGVVSCGRLVRGAFGKAGEMGHLP
                     VADSSIYCGCGSKGHAEAVFGADALGRSFREAGIRGDIPELWLRRREPGIARIWEEAI
                     DVLARTIAAAIHILDPQAVILGGGLARARDLADTVTPVILGYTAPPFRKTLDIRLSAL
                     GDDAAITGAAILGENAAAERPSTH"
     gene            complement(19699..21648)
                     /locus_tag="P5541_03150"
     CDS             complement(19699..21648)
                     /locus_tag="P5541_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRV71004.1"
                     /translation="MSILRRFSAVLLVVLAIAVPASGYERIISFNSSIEVQKDGSLSV
                     REDITVQIEHNRINRGIFRDFPTRYKDPSGKNVEVGFSVKTVLLDGGPVPWKTEKMSN
                     GVRVRIGDPNSYAPLGEHTYTVVYLTTEQAGFFEEHDELYWNVTGTGWEFAIERATAL
                     LTLPEGADVTKIAWYTGPQGSRAQNASGRMAGNTAFFETTRPLGPGEGLTIVAAFPKG
                     FVFPSEASLKRQSRALFIEKYGGIVPPLVVLLLFAYYMIVWIRYGKDIRPGHVIPLFY
                     PPEGVTPAMSSYVYHQKMKDDTFTATLIDLAVRGYMRIEEHDVPKGLFRKSKNRFTLH
                     RTEKDTGELKMVEKVFLRSLFPSGKPLPVDKESHSELLSAKRDIRDHISGRRKEFFLR
                     NWGWVATGLLATVLLIGGSGVLMGVATGDIGFVLVSMVIFTGWTIGVSVFVYNAFRSL
                     REGFKRRKFGQIIRGIFFSVFAIPFLGGELVVVGLLSEAATLPFALSFMAALVINVLF
                     FFLMKNYTPDGRKLMDYLEGFRMYMMTAEKERIRALAGVDMPEDTPEHFESLLPYAIA
                     LGVEKEWASRFEDVLKAAEYNPGWYAGTYPWYHMTASSFMTDLSGGLGSAIASSAVAP
                     GSGSGFSGGGGFSGGGGGGGGGGGW"
     gene            complement(21678..22232)
                     /locus_tag="P5541_03155"
     CDS             complement(21678..22232)
                     /locus_tag="P5541_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRV71005.1"
                     /translation="MTALWIVLGIVAVLGLFVMRTYNTMIRSRNLVDEGWSGIDVQLK
                     RRTDLVGNLVETVKGYATHERELFEKVTAARSAAVSAGSVTERAQAENALTGTLRSLF
                     AVAENYPELKANTNFLDLQQNLGTLEGEIQMARRYYNGAARGYNSLIQVFPNNLIAGP
                     FGFTKRDYFEAAEEDRAVPKVSFQ"
     gene            22382..22705
                     /locus_tag="P5541_03160"
     CDS             22382..22705
                     /locus_tag="P5541_03160"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRV71006.1"
                     /translation="MPGWDGRGPWGGTGPGTGWGLGPCGAGAMRGRGFRRAGRFGGGF
                     GYGRGFGPGWGAPWGAAPYGGYPAPWQNVDPADEKAALEDELGALEAQMKEVRDRLDR
                     IEKES"
     gene            22817..23176
                     /locus_tag="P5541_03165"
     CDS             22817..23176
                     /locus_tag="P5541_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRV71007.1"
                     /translation="MKIAVAFENGNVCPHFGHAPFYRIFDVSGESITGSTDHQSPGHA
                     PGVLPKWLSELKADVIIAGGMGPRAEELFVSFGIKPVTGVSGPAEEAVRSFIDGTLTQ
                     GKSSCGHVTGEAGDHHH"
     gene            23176..24030
                     /locus_tag="P5541_03170"
     CDS             23176..24030
                     /locus_tag="P5541_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV71008.1"
                     /translation="MPVKPFTIAVASGKGGTGKSCVAASLMVATPGLLGADADVEEPN
                     LSLLLGAVSISSSMVETVLPVVAERICNRCGKCIDACRFGALNMFGDSFPTPNDLCHG
                     CGVCSMVCPVNAISEKGRPIGEIRMNVKDDLKIYEGRLMVGMPNPIPVIHATIEAAMA
                     ERKNLVIDCPPGTSCPMVAAVRSADVVILVTEPTPFGAADLDLALQVVSNLEKPALVV
                     INRADLGEADIDSLAGKYNVPVAARLPFSRSVAESYARGNPPASIDPEWKKAMIGIRE
                     ISERMFRN"
     gene            24027..24887
                     /locus_tag="P5541_03175"
     CDS             24027..24887
                     /locus_tag="P5541_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV71009.1"
                     /translation="MKPKEITVVSGKGGTGKTSLTATLASFSPQKTVLCEADVDAPDL
                     EILLNPTLEEEHPFMGMQTATVDGDRCIGCGRCVDVCRFGAIGMTSQKAVIDKTFCEG
                     CSACALVCPQKCISMDDTRQGTWFRGQTDFGDMVHARLNPGGENSGMLVQLVRREAMK
                     TSERTGAGIILTDGPPGIACPAISAVTGADIALVVTEPTMSGKHDMLRIAGLCGRLGT
                     KVAVILNKADLSGEGAKKIEEACLENDLPLLTSIPFLIDVVHAVSQARIPSKELEDYV
                     KPVWDFLMEM"
     gene            complement(24853..25722)
                     /locus_tag="P5541_03180"
     CDS             complement(24853..25722)
                     /locus_tag="P5541_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRV71010.1"
                     /translation="MSDSKCEGCQSQGSCNQDPATCSMPVPTRGGFKHIIAVGSGKGG
                     VGKSSVSALLAIGLARLGNRVALLDADITGPSIPSMMGVSGMPKGTPEGIEPLASPSL
                     GIRIISMNLFMEDTSRPVVWRGPLIGNVISQFWNDTNWGDSEMLVIDLPPGTSDAPLT
                     VMQTIALDGIVMVTTPQDLSTKIVEKMMHMTRMMNVPLVGVVENMSHALCPHCGEKWE
                     IFGPSHTGEICSRWDIPVLASLPIDRNMSLLSDAGRLEDYAGGNQPVEMAEKIMSALG
                     SLVTSPSESPRRV"
     gene            complement(25740..26078)
                     /locus_tag="P5541_03185"
     CDS             complement(25740..26078)
                     /locus_tag="P5541_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRV71011.1"
                     /translation="MKIAIASEGPTMDSMVAERFGRCPFFLILDQDGNLEESMSNDAT
                     QAAHGAGGMAVQSIAAKGAKTLIAPRLGPNATMALKASGIRAFEASGCTCLEALEKFR
                     KGSLKEIPLG"
     gene            26535..26786
                     /locus_tag="P5541_03190"
     CDS             26535..26786
                     /locus_tag="P5541_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRV71012.1"
                     /translation="MPTTLCSMKNGQTAKIVMLPPGMSGRRLEALGLRPGKAVTKVSG
                     MPFSGPVTLILDGRQIAIGHGICCKVTVELFSAGGNQGS"
     gene            26783..28588
                     /locus_tag="P5541_03195"
     CDS             26783..28588
                     /locus_tag="P5541_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRV71013.1"
                     /translation="MKFCSHCPQGGCLRPCSNPDQEMSGLKPRKVLLVGNPNVGKSAL
                     FSRLTGVHAVSSNYPGTTVGFTEGNLFIKGERFRLIDVPGAYTIDPTNEAEEVARRIL
                     EEEADIVVITIDATSLERNLNLALQVLELGHPSVVALNMFDEARHEGVNINVEALEKE
                     LGVPVIPTVAVSGQGISDLVSRLEEARVAKNSVVSGKDRWARIGEIVSSVQTIGHRHH
                     SLRDRIEDFSVHPGWGAVLGVLAILGSFLAVRALGEGLIDFVLDPIFENLFMPLFAWI
                     GSLLGGEGSFLFRTLIGMTIDGEIDPEMSFGLLTTGLYVPLVMVLPYVVAFYGVLSFL
                     EDVGYLPRFAVLFDALMHRIGLHGFAIVPTLLGLGCNVPGIMATRILDSRRERFIAAT
                     LISVAIPCAGLQAMIIGALGDLGGRYVLAVYGTLFLSWVILGSILHRFLPGFSPELVI
                     EIPPYRLPSFSGIGFKLWLRVKGFLIEAIPLVLVGVFVANLLSTSTVMHTVSAFLSPL
                     FRGVLGLPPETAGPILLGFLRKDIAVGMLIPMRLDPGQMVVAVVTLAMTFPCIATFIV
                     LWRELGLRDMLLSIGIMILAALVAGGLLNALFALA"
     gene            complement(28597..29367)
                     /locus_tag="P5541_03200"
     CDS             complement(28597..29367)
                     /locus_tag="P5541_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71014.1"
                     /translation="MKTLLSLRGLGVTLGSREVLQSIDLDLNAGEIIALLGPNGSGKS
                     TLLAAAGGIGRKDRGSVFLLGDDLAGLPRREVAKRVAVVPQQTGFFMPFTVMETVLMG
                     RFPASGRFARLSPADADMARSALEKVDLSSFEEMLVTDLSGGEAQRVTIARALAQDTS
                     VLLLDEPVSALDPRHAMGVFRLLGELREKGKGIIVALHDINYALRFADRIFFLKDGVL
                     FEPRSPGLVDGDLLARIFDTGWRLETLPDGERAAFPLA"
     gene            complement(29364..30332)
                     /locus_tag="P5541_03205"
     CDS             complement(29364..30332)
                     /locus_tag="P5541_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV71015.1"
                     /translation="MNRSSFRILFSVLVFFLFASLVWVVSTGEVPITIREIVRVILGR
                     IPGTDPRSVIILSIRLPRALTACAVGAVLAGSGVVFQGVLLNPLAEPYTLGVATGAAL
                     GASIAITLGMPFVSPLAFVGGVAALGLVWILGHGGRESASPARLILAGVIVGSILGAC
                     ITLVKALSGDQVGAIVLWLMGSFSLSSWGDVGWSFLSALLVFVLGLFWARDLDVISSG
                     ANPRSLGVDDRKTVCLLLFGCSLATAVAVSISGIIGFVGLVVPHLCRLLIGPSHARLV
                     PLSVTGGAVLLLLADSAARTLGELPVGVITSLIGGPIFCFLLWRQR"
     gene            complement(30340..31212)
                     /locus_tag="P5541_03210"
     CDS             complement(30340..31212)
                     /locus_tag="P5541_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71016.1"
                     /translation="MFLLLTGDTSAGDNPSCRLASHEEADFIWWGFPVPEKVRDLPGD
                     FPVLPSMLTRAARLVSDFPMIIVRAGSFTPPRSPYMETGAVPGGKPGERAAVKDSERL
                     WDCGFSIGRLLSRMGEDLVIAESVPGGHETASALIVSLGLDDPQGSQEEIPGFSRGCF
                     SGRGFEAVTAVGDPFQIIAAGILAGAKDRAEIVLAGDIRMLAVAALSRDLGYRGRFRL
                     VTTGFSAPGLMPLADRIGVDVTGMEAGPVQDGAGAGGAVWYAEKLGISQEKVLLRAET
                     LRKEISLLSAAAGE"
     gene            complement(31197..31796)
                     /locus_tag="P5541_03215"
     CDS             complement(31197..31796)
                     /locus_tag="P5541_03215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRV71017.1"
                     /translation="MAVAFFDGASRGNPGESSAGACIIGEDGEILWEECRALGVGTNN
                     EAEYQALIILLEEIKKRGLRGTIVRGDSRLVISQVKGDWKVREERLKGFAGRARTLVD
                     ETGAVLEWIPRERNSRADALSNRALDGEKVPAASSFPEERLERVSGSIFIAHGSSDYA
                     VDVLHSSCSCPSFRARGRCKHLDAASRVTEREKKNCSSS"
     gene            complement(31833..32213)
                     /locus_tag="P5541_03220"
     CDS             complement(31833..32213)
                     /locus_tag="P5541_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71018.1"
                     /translation="MKRNSFALCAALFFCLLVAMPVFALGGSRVLDLRNTTGRMVWVT
                     KSDYQQFNPTQVQWSSLASGQIGIWNVLEGGWLHVGYASSSDQGKIERIFSFQLPYHP
                     DSRWIWKIIPQGAGLGLEADWGRL"
BASE COUNT         7171 a         9289 c         8366 g         7361 t
ORIGIN      
        1 cattccggag aattttcgcc ctcggctcct cagatgacaa tgtcatcgcc acttccgccg
       61 agcactatct ccccggcctc ctcaagagcc cttatcatgt tcactatctt ctgctgagat
      121 tcctcaacat ccctgagcct gaccggaccc atgtattcca tctcttcctt gattatctcg
      181 actgcattgg aggacatgtt cctgtagaat ttttctctca tgtcatcgct gacacccttg
      241 agcgccatgg cgagttcctt gttatcgata tctcgcaggg ctttctggag ggagcggtca
      301 tcgaccttga cgatatcctc gaaggtaaag agtttctttc tgatctcctc cgcaagttcc
      361 ggatcggtct cgtcgaggat ctcgagaatg ttcttttctg tcttcctccc ggccctgttg
      421 attatctgga caacagaatc gattccgccc gcaatggagt agtcccctcc cacaacggca
      481 ctgaactttt tctccagttc cttttccgtt tccctgagga catcgggggt tatcctgtcc
      541 attttcgcga tcctcaaagc cacgtccgcc tggtcctggt tgggaagacc gctgaggatc
      601 gccgccgctt tttccggggg aaggaaggaa aggatcatgg ttatcatctg ggggtgttca
      661 ccctggagga acgacagtat ctgtatagga tcagaagccc tcagaaaatc gaagggtttg
      721 gcctgaaggt tggaagtcag ttttcgaagc agatcctctg ccttggcctc ccccattgat
      781 tgctcgagca gttctctggc ataggagacc cctcccctgt caaggaactc ccttgccatt
      841 attatttcct gggcttccgt gagaaccagc tccttctgac ccggatcgat cctcctgacc
      901 gccgcaatct ccagggtaag tatctctatc atttgttcgt ccaggtgttt gtagactttt
      961 gatgcctttt cggggcctag aagaacaagc agtatggcgg cctttcttct gcctttgatc
     1021 tcatgcattt ccgccatctc tatgactccc ccaaccattg tctgattatc ccggccacat
     1081 cctcggggtt attttcagcg taaacgccca gttgttcctc aagaatctgt atgccaccct
     1141 tctttgtcct gaaaaggtca ggcatttctt cttcggacag ttctttttct ctcttgtctt
     1201 ctgttactcc acgctttctt tttatgaagg ctctcgaggc aaataccagg accgcgcaac
     1261 cgataaccac aggcagaaac cacaggggaa aggcaaaacc acgcccctgc tggccagcga
     1321 catcagcggg gacctccgca gccgaggctt ccggaacaaa ctcaatgaac gagagggtca
     1381 acctgtcccc cctgccttca tcaataccta tcgcagacga aagggaagca agaagctggt
     1441 cggaaggcaa cgaaccctcc tccccgttga taacaaccga ggcagttatc cgtttgacag
     1501 atccgggggt ctcctcctcc tgctgttgaa tggaagaaat ttcgtagttg gagatgtcgt
     1561 cggtcttgcc gtattcccct gaccctccac cactgctctg gactgcgtaa ccgggaatgt
     1621 tgacggcagt accaacagcc tgggttgacg ccgaagcacc tgtataggtc tcctcggtct
     1681 tgtgggcact tctcaggatg ccccccttcc ccccgggcac tggtagatat tcctggcgag
     1741 tctggttgcg gcgggtaaaa tcaagttcga tcctgatcct cacaattgaa ttacccgggc
     1801 caagaatggc agacagcatg ttctggatct ttgtctcaag atccctttcc tgcatttttt
     1861 ccagttctct ctgcatcgag gaaacccccc ccgttgagcc ggggagcata aaaaaggagt
     1921 cttccaccac gtccgagaga agcctccccg atgtgtcgac aacggagacg ttcgacggtt
     1981 ccagaccctc gacgctcgag gctgtcagat tcattaccgc ccttacctgt tcacgggtga
     2041 tgtctctgcc ccttttcagc tttagcagga ccgaggcaga tgccggcatc tcatccttca
     2101 gaaacaatct gggcttgtga agcacaacgc tgaccctggc tgtctcgaca acatcaagct
     2161 gtcttattgt tctcgcaagt tcaccctcta cggcccggat ataggctacc tgctgctgga
     2221 aatccgtcat gcccatcttg accttgtcga agatctcgaa acccacggtc ccacccttgg
     2281 gcatcccctc cgaggccagc atgagcctca ggtcataaac tctctcttcc ggaacaagta
     2341 tggaggaagc cccggggtcg agcctgtatt cgacattatt ttccttgaga aattccacta
     2401 tggcggcctg atctgaaagt tccagacccg aatacagagg aacaagattt ttaattcctg
     2461 caaaaaacac gaccccgccg aggaaggcca gaagaagaaa ggtaacaccc gcagcggtga
     2521 aacgcctggc cctgctcagg gaagcccaaa aaattcggat accggaaagg atttccttat
     2581 agttcttcat caggccggga atacctccgc actagactga gatcctaact aactggttat
     2641 aggcgtcgag gagcttgtct ctgatctgca cggccagcct gagggccata tccgcctccg
     2701 aaaccgcagc ggcaacttcg gagatatcct ctacatctcc ggtgaccatg gccttcatcg
     2761 cttcctctgc ccttatctga acatcattga ccttctcgag accagtcctg agggcatcat
     2821 caaaaccgga aacttttttt tcaccgttca caggtttatt ttcgttaacg aatctgttct
     2881 ccacgggctt taccgaactc ccgtatgcca ttcgggcgtt tccaatcaac gagaggttta
     2941 tcatggacga gcgcaaatcg atgaccctcc tttttgccct ggtatttctc cgcacacctt
     3001 catggggcga tctccatacg tgccgatacc aaattttgct gcagttttgc gagaaccctc
     3061 ttgtggagct gcgatatccg ggattcagaa agaccaaact cccttgatat ctccttgaag
     3121 ttacggtagt tgaaataata aagctgcacc agccgccttt cacgctcggt gagcatttcg
     3181 agggcctggt gcagctcttc aatttcctct tttcttatta caagttcttc ctggtcggga
     3241 cccgggtcgc atagtatgtc cgcttttcct acttctgagt catcaagggc gaccgtttca
     3301 tccaggcatc cgacaaaact cctgtttgaa atctctgtaa gctcctgata ctgctctgga
     3361 gaaagaccca gccgttcacg aaccctgtcg tgatcgactg aaccgttatc ccgcatctct
     3421 tcttccaggg ccctgtccag ggcatgaatc ttctccctgc cggaacgtga gatccagtca
     3481 aaacgtcgca gttcgtcgag aatggccccc ctgatacggg aagcagcgta agtagggaac
     3541 gcaaaaccga gagaggggtc aaacctgtcg atggcatcca gaagacccat cttgccataa
     3601 ctgatcaggt cctcgtaatc gagctccctc ggaggggtta cattcatcct ccgaacaatg
     3661 aacctaacca ggtactggta cttcctgacc agttcctcct ggatgccctt tctttcgggg
     3721 cttctgggca gggtcagacg atagttttcc cagatacttt cttcctgccc ccggacttgc
     3781 gccgaagtgg aaacatgttc ttccatcggc cacctctcct cagtaaataa tattataaat
     3841 aacataataa ctaacataac tacggtgttg atattataac attatttgcg tttgcgcaag
     3901 gggttttata tcggatgttc caattccaca aattagaggg gtcccggtga tgtcgggttt
     3961 ttcacagggg ttttttcttt tccagggagt agacgtaagc cagaacttcc gctaccgcaa
     4021 cgtagaattc ctccggtatc tcctccccga tattaattct ggggtacaac cccctggcca
     4081 ggctcttctc ctcgaccaca ggaataccgt tcgtctctgc tatctcccgg atcttaaggg
     4141 ctacgaagcc ggcccccttg gccagaacaa cgggagcacc catttttttt ctttcgtact
     4201 tgagggctat ggccaggtgg gtcgggttgg caaccacgac gtccgcctcg ggcaccctgc
     4261 tcatcattct gtgctgcccc agttcttgct gcttccttct tattctctgc cttaccaggg
     4321 gatcaccctc agtctgtttg aactcgtccc tgatctcctg tttgctcata cggatcgatc
     4381 ttgaatgctc ccagcgctgg tagatgtagt cgaagagccc cagaacaaat agagcaagag
     4441 cgagtctcag cgcgagacgg aagagaaggg taaagatgaa aacgattccg acggaaacat
     4501 ccagccggga tgcttcataa aggataatga actcgttttt cagtccaaag aagagcacgg
     4561 caaaaaggat ggtagccttg aggaccgcct tgaaggcttc cacaaaggac cggaccgaaa
     4621 acattttctt gagtcccttg acgagactga acctgtccag ttttgggatg agcggttttg
     4681 gggagatttt caggccaacc tgcagggaaa ccaccagtaa ggcagataaa agaaccacca
     4741 gggaaagggg gagccagatc ctgaaaaaac agagcacccc gctcccggga agccggttga
     4801 cccagtcccc ttttccctgt ccgatcgacg gcaacagtcc tatggtatcg gtcaggaagg
     4861 acctgtagat tctccacgcc agacccgaga aggcaaaaag aaagagggtc ccagtgacaa
     4921 gcattaccga agatgtaagg tccctgctat gggcaaccct tccctcttct ctctcttttc
     4981 tttccttttt cggagtggct tcctcggtct tctcctgaga gaagatctgc agatccatta
     5041 acctcacggg cgtccccttg tcgagggata ccacatcgca gccaggttta attatgttta
     5101 taagcatgtt acttattata atatatttac caaaatacga aagagcctca agatagatag
     5161 tccacaaggg tgggctggac aacccgcgaa attgtcgcaa gggtcgcctg gtaaatcatc
     5221 tgcgaagtct ggaactctat ggaagcctcg gcaagatcca cgtccatgat gctgctgtga
     5281 agatcggtca tggctatgtt gttggttctc atcctgctgc cggaaatgtc atatcttctc
     5341 tggatagccc cgcaataggc ccttgccttg agaacgtcgt cgataccctg gtcgagccgg
     5401 ggaagaaaac tgttcgaaag gccctctgag tcaccctgcc tgacggcaaa gatgagatcg
     5461 tcaagtatcg cgaatatgtc tgcctcttca acatcggcgc ctctccgcac tgtaactgtc
     5521 tgtgccatgg ggccacccgg tcctcccgcg ctcctggacg gcgcttcatc gccagcagga
     5581 aggaccattt ctgccggggc ctggatcctg aaagagtttc ctcgtggaga aaagaagacc
     5641 agttcttccg tcccgccgtt gttcacgaca cgggccttca gatctccccc cgcgaaacgc
     5701 atgttcacat ggtttgccac accttcaagg gtcgtctctt ctttcaggtc tatctcgaga
     5761 gagtaaccgt cgatctccac cctcagtgtc ccaccgcccg tccatgtgcc cgcccgggcc
     5821 ctgacatcgg tgttgaaacc gaagtcctct gccgcacttc ccgtgacatc gtatatgttc
     5881 agcggagatc cgtctttggc cgccagcgcg agtcgcatgt tcccggaccc gtcggaggcc
     5941 agcgacacat caagccatcc gccggcaagt tccccgaccc gggaagccac atcctctatg
     6001 gtttccgaag accctacaga gacggtgatc gaacgccctc ccgtgcagat cgtgaaggaa
     6061 ctttcggaac cggcagcaag tgtcggatca acaggttcac ccgtgactcc cgcatgcaga
     6121 ccggccgcca tggcaaaacc agatgtggcc cctgccatgg aaggatcact gtaggggaga
     6181 tctatgaacc tgacgggttc ccctgttgcc gaatagaaaa caaggctgct gcctgactgg
     6241 actgcctctg ccgcgatcag ttcatccccg gtctgtccga taatctcccg ggccagggcc
     6301 tgggaatcta tggtcccgtc gggaagggca atatctcttt tgtaaagttt gacccggtaa
     6361 atttctcccc ccgcctcgac accaattaaa agaggcacat ccgggggcgc cgtgccggga
     6421 aaggtcaccg tggagaggtc ggacgtggtc aatgcgctgt ccagccccag agaccttgcg
     6481 tacgacccct ccctgtcgaa aatcgcaagg ggtcctccgt ccttggcctt gagcagaagt
     6541 ctcagtgttc ctgtctccga gttcagggag gtgctgtctg tagggctaac ctcgggctgg
     6601 tcggattcca caacaacttc aagccagtcc cccgccgccg ccctgatccg ggccgcgaag
     6661 gtctcgaggg taagcgcggg gtcatccgcg atccggagat tcagtctttt ggtcccggaa
     6721 gagaacacca tgtccagcgg actggctgtg gtatcaccga tcacggaaga aatctccgcc
     6781 tgggtgctga cagcatgggt ctcgagccct agaaaagatg aaagaccgag atggctgata
     6841 tccggttcga cggaaacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     6961 ctgcatcggc tctcccgtga ggctcctgat gacaagccgc tggatacccg aaagggaatc
     7021 ggtttccacc gacgcgttga caaggctccc ggcccccgcg gaatcattca gcagttctgc
     7081 aatttcgatg aggctgtacc cgttcagggg ttccccgggt gatctgaatc catcatgatc
     7141 cgaattgtca ctgagggaat cccggttgtt gtcgatccac cgctggggta cgggaacctg
     7201 aaccgttctg tctccaacgc ttattcgaag gttttcggaa cttcccgtcc acctgaagtc
     7261 catgggaagt tcgatgcttg ttaccgaatg cctggtgtat cccaggggga agacgtccct
     7321 gccatttaga gaaacgaggc cagtctcccc tttttctatc tggaaccgta tccttccgtc
     7381 gttaccctgg taggtcacgg ttccgtcact gttccgggaa aaggcgggtg acgccgtatc
     7441 gaaacccgaa agaaggtaac gcccctcgac atcaaaatta gccgtctgga ccagttcgtc
     7501 cctgagagcc tcaatatcct ttgctatggc atcaaggtcg ggtctggaaa gggccccgtc
     7561 gcaggcgtag atgacctttt cccttattgc cgacacaata tcggttatct ggttaagtgc
     7621 cgtatccgtg ttggaaagcc aggagactgc atcgtccagg ttcctctgga actgctcgtt
     7681 cctcgcaagg gaggtactga cagccatacc ccgggccact tccgacgggt tgtcggaagg
     7741 cttgctgtac tgtttctggc tggctatcct tttctgtatc tgcatgagcc tgttaaggct
     7801 gccatgcatg tcctggagaa acacactgga catcatcgac tgggagatcc tctgcaccaa
     7861 aaccacccct tggggtcaga ccttgatttt aaccttttgc actaaaatat taactatctc
     7921 ttcaggttca tgagttcttc aaggatctgg tcactggtag ttattaccct cgccgaagcc
     7981 tggaaacccc tctgggcctt gatgaggttc acgaactccg aggtgaggtc cacgttggat
     8041 gcctcgaggc tcttgcccgc aaggcttccc gcaccaccct ccatgggctt tagtatctgc
     8101 ggtatacccg agttggccgt gtccctgtag gcaccgttcc ccattttttc cagcccgctg
     8161 gggtttgaga agaccgccac ggcaagagtg tagagactct gggtctgacc gttgtcgtag
     8221 atcccccgga tgatcccgtc ggaacccacg gaatagtcct gaagaacccc cattctgtag
     8281 ccatcctggt agtactcctt ggtcgtgaag gcagagccga actgggttac cgaatctgtg
     8341 gggctcaccc ccatagcctg ccccgtcagg tccagcagta tctgggagtt gggtgctccg
     8401 agggaagcaa aatttatggt tacagtgctg gggccgctgg aataatctac aagccccgag
     8461 gggtggaact ccaggagacc ggtgttgttg cccagagaaa caccgggcgt atcggggagc
     8521 catgccctcc agcgccagac gttgttgtcg actttttccc agctcgtctc gagagtgtag
     8581 gagttcccct ggccatcgaa gatgttgaac ttggtgctgt ggaggctggc agtctgaaga
     8641 tcgaagttct tgaccgatgt cccaccctgg gaaagggcga tacccatacc aagtgttgtt
     8701 gaatcgacat ccagcgtctg gggagttacc ccccccgtgt tgagcgttac cgtcgttccc
     8761 gcgggaacaa tgaaagtccc gtcgtcatct gcatccaggt ccagttgcac cctttccatt
     8821 gcaggggtgg tagcattgtc gttaagtccc gtaagaacaa ggcttctgcc tcccgaagcg
     8881 atgtcgtcga agtgtgccag gtactgcaat gtgccacctg agccgtcgct gatgttaacg
     8941 acatggtagt ccagggcggc accgagatca cccgtccaga gaaccgtgcc cccactggag
     9001 actacactta cctgtcccgt agtatcgtca aaactgatag tgtcattcga agccggcccg
     9061 gaaaggtctg ccgtcgtcgg ggtcaatacc ggaagaccaa aggtggggtt gattccagct
     9121 atgggcaggt tgaccgttgt ctgagatccg gcggcattgg tgaaggtcgc cctgataaaa
     9181 tcgttcagac ctgtgccttt tgagaaagtg atgctgtcgt actggacatt cccgatggtc
     9241 ccctggaaaa cgacatcgtt gccgcttagc cccatgggta gaatggttcc cacccgggaa
     9301 tcgaggttgc aacggaaacc ggcggtggta gtggcctttg ccggtatctt ttgtcccacg
     9361 gggatattga tatcattgag gcttgttccg actatcttgt tgccagggtt cagaggatca
     9421 tcgctcatgg tgaatccctg gagccttgag cccgtgccac tctggaccat gtcaccgttc
     9481 ccgtcaagaa tgaaggcccc ggcacgagtg tagaccttgt tggaaccctg ttccaccacg
     9541 aagaagccgt cgccctctat ggctatgtct cccttggcac ccgtgtagtt gatgttgccc
     9601 tggctatgat cgacaatgac gctgccgatg gacattccga gaccgatctg gttgggattt
     9661 gatccgccct tttccgctcc aggagctgtg gccgactttt cggtctggct catcagttcc
     9721 tgaaaattca cgctggattt cttgaaaccg gccgtgttgg cgttggaaat attgtctcca
     9781 accacgtcca gaagggtctg gtgcccttta actccgctta ctcccgacat aagggatctc
     9841 aacatcgccc tttacccccc gttttcccat tcccaaacga tccctgtccc agtcttcccg
     9901 ccttccctgg cgggaacatg atgcatcaca ctatcctatc cgggagatcc tgtttataga
     9961 tacttcctcc ccgctgtcga cctttacgct gatactgttc ttttcgaaca acaccgactg
    10021 tacgacaccc tccctgtccg ctccgtcatc atccgcaaag gtgacaacag acccgaccag
    10081 cgaagccgcg gaaaaacgga tgtgttcccc aaggttcgca agtttatcgt taagattggc
    10141 cgtctgttca acttccgcaa attgggccat ctgcgcaatg aattgcgccg agtcaagagg
    10201 ttcaagcggg tcctgattgg acatttgcgt taccaggatt ttcagaaagg catccttgcc
    10261 caggaggctg ttgcccgcgg ctttttcagc cgccgcacca gtacctggaa gggaaccgga
    10321 agtcagttcg accgatgaag tactcataaa agccatctcc tttcgtgctt gcttctcctt
    10381 tcatttatcg gcaaccgtag ggggataaaa cagggaaggt gaaaaacagt gatcgcgata
    10441 atggttaaac tgcgggaact taccctttat ttgcgccttt cgatttttcg taggcaatac
    10501 gaccgttctt tatttcggcc gtgttgccgg ggaccttttc tagtacatca atgaaggggg
    10561 cttctattga cgctcccgat attttttcgg cgatctcgat cgcgcgagcc gcatatcgca
    10621 gtatctgcag ggtctcgatc cgggatatgt cgtcgaagaa ccatccgcaa ctggtgaaca
    10681 tgagcatgga aaagcgctgc atctcaagaa gatcgtgggc ctcctccgga agggctttgc
    10741 tctcacccga gaggatccga tccatgtagc catccctggc ctcccatggg tccgggaaaa
    10801 atcccgagcc cttctcttcg aaaagaggat ccaggacttt tctgagcccg tccagggctt
    10861 ccctcagagg ggctctccat ttctgattcc attccgggtg gcctcccgcg gtacatccgc
    10921 agtcggatct ccatctttcc acgccatgga cacagctcca tgaagtattc tcggctatcc
    10981 tgacctcatt ttgcggagga aacctatcga ggtgttcgcc gtatacagtg agttgagctt
    11041 ccatgccggc ctcgatggat tcgagacaat aggcaagggc catttccccg aatttgtgat
    11101 ggtggccaaa ggtctcgcca tctacagcag cgtgggaaag gcccggggac tttccgacag
    11161 cgtttcctgt ccctgcaagc cgtgaagcca gcaccgcacc atcacgaagc aggtccccga
    11221 aagcgatttc atgggctatg gcaccgtcgt aaaagaatat ggctatttcc cttccggaag
    11281 gaagatagca gacataggga accgatgtgt cgatcccgcc gcctgaaacg tcccgccatt
    11341 ttccaccatg accgcgaacc tccagagcct ggtggggcgt cagtatcgtg aagagtattc
    11401 cttcatctgc cattatctcc agggtttctg tgtctacggc ggtctccgga agccacattc
    11461 cctcaggctt tcttccgaaa cggtgtttaa aatcggacac tccccataac acctgggttt
    11521 ttttgtccct ggcactggca agaggcatta tcatgtggct gtagcactgg gctattgctc
    11581 cgccatgtcc ggagaaacgc tttcggcttt tgttgtccga ttcgattatt ttcgtgtaga
    11641 gttcgggccg ctccctctcc atccacgata gaagggtggg gccgaaattg aaactgatca
    11701 gggaatagtt gtcgatcctt ttgagcaccc ctccatcggg gccaagtatc cgggccgacg
    11761 cgttgggttc gtagcactcc gcggtcaccc tttcgttcca gtcatggtat ggagcggcgc
    11821 tttgctgaag gtctatctcc ccgctccagg ggttctctct tggaggctgg tagaaatgac
    11881 cgtggatgca gacgggtctt ctggattctg tcatgtctga aaaacctcca ggggaaatat
    11941 taagtcgtga atcgtaagtc gtgaatcgtg aatcgggagt tcaaaaacag atccttcggt
    12001 cgctgcgctc ctcaggatga caagcccctg ccctcccgtt taccgtttac cgtttaacga
    12061 tctccgttca ccgttctcgc ctttctcctg cttcatcagt attattccga gcgggggaat
    12121 cgtaagggcc acactgaagg ggaaaccgtg agatggctca ccagtagcgg ccacaccacc
    12181 catgttgccc tggccgctcc caccgtagca ctccgcatca ctgttcagca tctcttccca
    12241 ccgccccccg gacggaaccc caagcacata gccatgcctg ggaacaggag tgaaattaca
    12301 gactgccact acagcctcct cgccaccgaa tcgcaggaag gatacgatgc tcgaatccct
    12361 gtccctggca tccagccagc tgaacccctc gtgaaagcag tctttctcgt gaagggcggg
    12421 ctctcttctg tagattctgt tgaggtcact gatccacagc gttaccccgg cattcggtcc
    12481 catgccggca agatgccagt caagggatga ttcgtggtcc cactcacgac tggccgcaaa
    12541 ctccccgccc atgaatatca gcttctttcc ggggtgggca tacatgtacc cgaggagaag
    12601 tctcaatccg gcgaatttct gccagtcgtc tccaggcatc ctggcaaaca gcgatccctt
    12661 tccgtggacc acttcgtcgt gggaaagggg aagcatgaag ttctccgaga aggcgtacca
    12721 ggcagagaaa gtaagcaggt tgtgctcgaa tttcctgaaa accggatcca gcgaaaaata
    12781 cctgagggtg tcatgcatcc accccatgtt ccatttgagt ccgaagccca gccccccggt
    12841 gtggaccggc ctggtcacca tgggccatgc ggtggattct tcggctatgg tctggatccc
    12901 tggaaaatcc ctgtacagcg agacgttgag atctctcagg aactgcatgg cctcgagatt
    12961 ctccctgcca ccatggatgt tcggaaccca ttcgccctct tttctcgagt agtccagata
    13021 aagcatggag gccacggcat ctaccctgat cccatcggca tgatacctgt cggcccagaa
    13081 ggccgcgctg ctgagaagaa atgaccttac ctcattgcgg ccgtagttga agatgcagct
    13141 tttccagtcc gggtgaaaac cctgccgcgg gtcactatgt tcgtaaagac aggacccgtc
    13201 gaaacgcgcc agcccgtgag ggtcatcggg gaaatgagat gggacccagt ccaggatcac
    13261 cccgatcccc gcctggtgga gcacgtcgac aaggtactta agatcggagg gcgtgccata
    13321 tctcgctgtc ggcgcaaagt accccgttgt ctggtacccc caggatccgt agaaggggtg
    13381 ctccatgaca ggaaggagtt ctacgtgggt gaagccggtc ccggaaatgt agtcgaccag
    13441 caagggtgcc atctgccgcc aggagagaaa ctcaccgtct tctccacgtc tccatgaacc
    13501 tggatggatc tcgtagacag agatgggtct gttcagggca ctggatcccg aacggttttc
    13561 catccattgc ccgtccttcc agtcatgggt atcatcccag atgaccgagg ccgtccccgg
    13621 cggaggttcc atgaatcggc ctaagggatc tgccttttca ccgctccatc tgccgtctga
    13681 agacttgatg aaatacttgt acctcgctcc atggaccgcc ccgggaacga aaccttccca
    13741 tattcccgag gaatcccacc ttggtttcag cggagatgaa cctctgtccc agccgttgaa
    13801 atctccgatg acggatactt ctcccgcgcc gggagcccag acggcaaaat gggtaccttc
    13861 cccctccggg agatcagtaa aatgggcgcc aagcctctcg tagagccggc agtggcgccc
    13921 ttccctgaaa agatatatgt cctgctccgt cagaagcgaa gtgtcatagc gcattatgct
    13981 ttcccgtttt ctttcaggcc atggttcact ccttctggat catcccgtaa aaggaacccc
    14041 ggccggagtg gtcggaatag gaccacttgc cgatgaaacg gttcatgaag tctatccttc
    14101 ccataaagta aaagggaacg gcttcttcac cctctgtaac gaactcgcat tctccgtttg
    14161 gagcacaggt tccctttacg tccatatcca ggaaggagac gttggaatgg acagtaccgg
    14221 tgatgctccc gtcgggcccg acatcgatca ggaacgatcc gtaatcatcg ccgatgaagg
    14281 tacccctgta gttccctaca agttcttctc caaccgaagc gaaagccgta ccggaaaatg
    14341 ctatcgccac cacgagaacg gcggtcagga tatttcttct tgctgtcatt ctcctccact
    14401 ccctttcttt tttgacatct acccggagac agaatgattc tgtcatcctt gagcctgact
    14461 atgcttttaa accaccgaaa cgccttctgc acctacgcct tcccatacca ggcggaaact
    14521 cttctgtctc gaggatgccc ccctcgcggc aatccatgca tagcccggcc atttcgaagg
    14581 tctggggcag aagatcgaat ccttcttctt ttgcccagcg gaggacctcc tccatctttc
    14641 catcctcgtc ggaaaggtga accgtcctcc cgcaggatct gcagaagata tgaaaatgca
    14701 tcctgtcatc gggaacctcg aagcgcagaa acccttcttc aaggttgacc atgttcagca
    14761 tccccagcct gttgagaagc tctatagtcc tgtatattgt ggcaatacct atcgaaggat
    14821 cctcgacctg ggcgagatca agcagttccc gcgcgttcag gtgtgtcccg aggtgccgca
    14881 gaagggtctc cacaatgacg cgtctctggt tcgtaagcct gaagccccgg gacttgagcc
    14941 tgccaagata ctcttctatt ccttcgctta tccgttcctc attcatgaca tctctcccgt
    15001 tgccggatca tctgaacatc agttccttgt cggcgggata ggtcacagtg tatttgacgc
    15061 ttatctcgct gctctgacct ttttccagtt ccacattcca ggtcaccaga cctttttcta
    15121 tcttctcggc cggctcaggc gaaaccacaa gatcctgtag tttaatgttg tccctggcgg
    15181 gaacgggaat tcggtccata accttgaggg tcaccttctc cctgaggcca ttgcttaccg
    15241 taatgacata tcctcgctgg tgttttcccc ttatgatcca gctgctgccc ttctcgggaa
    15301 gcatctcttc ccttctggag gtcaccagag gtgtggtccc gaaaggtatc tgtagttcct
    15361 gacccatgct ccgccttggt atgttggtaa cccctgtttc cttaccgtca accgaaagcc
    15421 ttgcctcacc gggaagaatg gaccttccaa gggattttac ggtggaaacc gtccatgcct
    15481 cgctggaaaa ttcgggtatg gcaacaaggg agacctcgct tttttcggag aatttgccca
    15541 attccagcat ctccttcttg ccgaaaccgt ttacgattcc cctggccttc aatgttacgt
    15601 ctgtcaggcc ctcctccatc aggggttcct gtggaactgc ctcatccatc atcaccgatg
    15661 ccgccatctc ctccatagct ccccgcatgg ctttaggtgc cggggagatt tccagaaaat
    15721 cgacgaccag ggggcgcatc tcgggtatgt tgataccacc cttgggtctt acggtgtgaa
    15781 gaaggatctc cccattccag gttataccgc tcttctggct cacgtcggcc gcaagcttca
    15841 ccgtcacctc gcccgtcgag ctgttgagtc ccattctgta ggaaggtctc cagtaggcct
    15901 ccctcgtcca ggcttcaaga tttatcttgc ccttgccctc cgtctccagc gagagaagaa
    15961 gcaccctgtc gggatctccc ggaaactggc tttcgtattc ggagcggagc aggtcatgct
    16021 ctttcatggc cttctccaga aggatctcgg tctcggccgc cttgagttca acggattccc
    16081 tttttttgac cgccgtgtcg acgaaagctt ccagttccga tcccttgaga ttctcgggaa
    16141 cggcctcgcc aagatgtttc cttgcctgct ccagggccga ggccctggcc cttagcatgt
    16201 cgacccttga cctagccttg tcaagttcat cggccagaga ggcaagggca ggcgggaccc
    16261 accccggcct tgccgcctct acgatatcgt acccctggac cgtgactccc tcaccagcgg
    16321 ttactgtcat gtttcctgga tccagggtca gggggagttc tatctgcata aggggttcgg
    16381 cttccatttc gatggagagt agagcacccc tgggatagac atccaccttc cccgccgtca
    16441 aactgttttc ggcaccggaa gcagcgcccc ccgcggcgag aacagccgca acggtaaaga
    16501 ctgccagaaa cgatctccct ttgttcatga ttctccctcc cgatctggat ctttttgagg
    16561 tttacaaacc gcgcgtgaag ttccagactc tattatcata cacttacagg cccctgatga
    16621 taaactggca tataagaggt gaaaggaatg ttgaactgtg gcattacagg attgcccctt
    16681 agtggaaaga gtaccatctt caacgtcata acacgggccg gagcggaggt caagccctac
    16741 gctggcggaa agaccgaccc gaacagggct gtcgtttccg ttccggatga caggttcaat
    16801 agactggtcg agatctacca gcctaaaaag ataactccgg caccggtcga attcgtggat
    16861 ctggcgggat tatcccgcga cgccagcaag ggagccggtc tcggcaactc gttcctctcc
    16921 ttcgtggcgg aatcggaggc ccttattcag gtcatccgct gtttcgacaa ccccgaagtg
    16981 ccccatcccg aggaaactgt aaacccagtc cgcgactgga acatcgtcga gaccgaactg
    17041 atcctgcgcg acctgtcagt tatcgagaac aggcttggca aacttcagga aaagaagaaa
    17101 ccgtcccccg aagagcaggc cgagaaagcc ctcctcgaaa ggtgccggga tcaccttttc
    17161 gaagagagac cccttcgaga gatgggcttt tcccccgacg aggcccgaac tgtccggggg
    17221 ttcacattcc tgaccctgaa accggagctg gtcgtcctca acatggacga gacccaggct
    17281 tccggtacca tgccctggat gtctgaaatg gaaaaactgg ctgcagaccg ggggctcagc
    17341 ctcgtgaggc tatttggacg tatggagatg gagatagccg accttgaagt ttctgagcag
    17401 gcagagttta tgtccgaact gggcatcagc gaacccggga gagaccgtct cataagggag
    17461 gcatactccc ttctgggcct tatgtacttt ttcaccgttg gcaaggaaga ggtgaaggcc
    17521 tggactatcc gggaagggtc gacggcggtc gaggcagcgg gggccatcca ttccgacctg
    17581 gcaaggggtt tcataagggc tcaggttgtt cattatgacg attttgaggc caactccttc
    17641 tccaacgccc agtgcaggga gaagggactc cttcgccttg agggcaagga ttacaccgtc
    17701 cgcgacggcg atatcataga gataaggttt aacgtctgac agaaagggga tccgggaatc
    17761 atgtcaattc tttttgaacc tctcgaaatt cggggtatac ggttgaagaa cagaataata
    17821 tccgccccaa tggcatcaaa tacttgcacg aagacgggcc ttccgacagc tgacaccctc
    17881 gactattaca tgcccctggc tgaaagtgga gtcggccttg ccatcgtcga gcatcatgcc
    17941 gtacaccccg acggcagagc cagggaaaga cagctgatga tggaccgcga cgagatcatc
    18001 ccttaccaga aggacctttc cgggcttttt tcttccaggg atatccccgc gatggtacag
    18061 ttgaaccatg cggggtcaca gatcatggac gaggagctgc tggtcaacgg ctgggcaccc
    18121 aaaggccctt cgccgataag acaccccagg tcaaaggtct tcatcatgcc ccagggcctg
    18181 accacggaag acatcgcttc cctgccaggc cttttcgccg attccgcaaa gagggccgta
    18241 aaatccggat acattggtgt agagatccat gcatgccacg gataccttct gggccagttc
    18301 ctcagtccta tgaccaacct ccggaccgac gcatatggcg gagagataag gaaccgggcc
    18361 cgacttctct tcgagatcca cgaagcggtc cgcgacgccg tgagtgacga gacagtggtc
    18421 gcggtcagac tgggcctggc cgacactcta cccggaaagg agccctccgg tcagacaatt
    18481 gaggacagtc gatgggtggc aggcgagttc tcctcgagag gccttgacct tctggacctc
    18541 tcggggaaca tgtgcggata cgagggacag ggggaagcct ggttcgctcc gtactgccgt
    18601 accgtcaagg atgccgcggg aagtataccg gtggtctgta caggaggaat caggaccgcg
    18661 gacacggccg tcaggcttct cgaacgtgga gattgcgata tcaccggaat cggaagggcc
    18721 ctgaaagcca accctggtct ggttcacgac tgggaaaaag atcaatgacc tttctgggta
    18781 ttgacctggg cggacagttt ataaagggag ccctggttga taacgggttg atcaaagcac
    18841 gtgaaaaagt acctacacct gaagggaggg atcccgacgg ggtatccatc gcaatatcag
    18901 ggatccttga ccggctcgac ccggacagag acgctgagtc tgtaggagtg ggctttccgg
    18961 gtatgcttga ccaggaccgc gaaaaaatct ttcaggcccc aaattttccg ctccttaaaa
    19021 actgccactt cagaaaatat ttgcaggaga agacaaccag gagaattttt ctggaaaacg
    19081 acgccaattg cgcgaccctc ggggaatgga gatcaggagc cgctatgggc ctgaccgatt
    19141 ttgtcatgct gacacttggg accgggatcg gcggaggagt tgtttcctgc gggaggcttg
    19201 ttcgaggagc attcggaaaa gcaggagaga tgggacacct tcctgtagca gacagttcca
    19261 tttattgcgg atgcggctca aagggacacg cagaggctgt ttttggcgcc gacgccctgg
    19321 gaagatcatt cagggaagcg ggaataaggg gcgatattcc cgaactatgg ctccgcagga
    19381 gagagccggg tatcgccaga atatgggaag aagctatcga cgtcctggcc aggacaatag
    19441 ccgccgctat acatatactg gatcctcagg ctgtgatcct tggcggagga ctcgcccgcg
    19501 ccagggatct tgcggatacc gtaacacctg tgatcctggg ctacacagcc ccacccttca
    19561 ggaaaaccct cgacatacgc ctttcggccc ttggagacga tgcggcaatt acaggagcag
    19621 cgatccttgg agaaaacgcg gcggcagaac gaccctccac acactgaggt tacgcctgcc
    19681 gcccaattac ttgcctttct accagcctcc gccgccgcct ccgccgcctc cgcctccgga
    19741 gaagccgccg cctccggaga aaccggaacc tgatccggga gcaactgccg atgatgctat
    19801 cgcgcttccc agaccgccgc tgaggtcggt catgaaggac gaggctgtca tgtggtacca
    19861 ggggtaggta ccggcatacc agccaggatt gtattccgcg gccttgagaa catcctcgaa
    19921 ccgcgaggcc cactccttct ccacacccag ggcaatggca tagggaagaa gactctcgaa
    19981 atgctccgga gtatcctcgg gcatgtccac acccgccaga gctctgatcc gctccttctc
    20041 ggcggtcatc atgtacatac ggaaaccctc aaggtagtcc ataagtttgc gcccatcggg
    20101 agtgtagttc ttcatgagga agaagaaaag cacgttgatc accagggcgg ccataaagga
    20161 gagggcaaac gggagcgtcg cggcctcgga gagaagtccc actactacaa gttccccccc
    20221 aaggaatggg atggcaaaga ccgagaagaa gatgcctctg atgatctgac cgaattttct
    20281 tcttttgaac ccctccctta gggaacggaa ggcgttgtag acaaatacgg agacacctat
    20341 ggtccatccc gtaaatatga ccatggaaac aaggacaaaa ccgatgtccc ctgtcgcgac
    20401 acccattaaa accccgctac cacctatcag aagaacagta gccagcaggc ctgtagccac
    20461 ccaaccccag ttgcggagga aaaactcctt tcttcgcccc gaaatatgat cccttatatc
    20521 tcttttggcg gagaggagtt cgctgtggct ctccttgtct acaggaagag gctttcccga
    20581 agggaatagc gacctgagga aaaccttctc caccattttc agttctcccg tatccttttc
    20641 cgtacggtgc agggtgaagc ggtttttgct tttccggaaa agacccttag ggacatcatg
    20701 ctcctctata cgcatgtagc cccttacagc aagatcaata agtgttgccg taaaggtatc
    20761 atccttcatt ttctgatggt aaacataaga ggacatcgcc ggggtcactc cctccggggg
    20821 atagaagagg ggaataacgt gaccgggcct gatgtccttt ccgtaacgga tccatacgat
    20881 catgtagtag gcaaaaagaa ggagaacaac cagtggcggc acaataccgc cgtatttttc
    20941 gatgaacaat gccctggatt gacgtttcag agacgcctcg gaagggaaga caaaaccttt
    21001 cgggaaagcg gcaactatag tgagaccctc accggggccc agaggcctcg tcgtctcaaa
    21061 aaaggcggta tttccggcca tgcgtccgga ggcattctgt gcccttgacc cctgggggcc
    21121 cgtgtaccag gctatctttg taacatcagc accctcaggc agggtcagaa gggccgttgc
    21181 cctctcgatg gcgaactccc atcctgttcc ggtaacattc cagtagagtt cgtcatgttc
    21241 ctcgaagaag cctgcctgct cggtcgtgag gtagaccacc gtataggtgt gttccccgag
    21301 gggagcgtat gaattggggt ccccgatacg gaccctcact ccgttgctca ttttttcggt
    21361 cttccacgga acgggccctc catcgagaag gacggtcttt acggagaagc cgacctccac
    21421 gttctttccc gaaggatctt tgtagcgggt ggggaaatcc ctgaatattc ccctgtttat
    21481 ccggttgtgt tctatctgta cggtaatgtc ctcacggacg ctgaggcttc catctttttg
    21541 gacctctatg gaactgttga aggatattat cctctcataa ccgctggcag gcaccgcaat
    21601 ggcaagaacc accagcaata cagcagaaaa tcgtctcaga atactcatgg gtgcgcctcc
    21661 gtccgtccct ttgttcctta ctggaacgag accttcggga cagccctgtc ctcctccgct
    21721 gcctcgaaat agtcgcgctt tgtaaaaccg aaaggcccgg cgatcaggtt gttggggaat
    21781 acctggatca gggagttgta gcctctggcg gcgccattgt agtatctccg ggccatctgg
    21841 atctcaccct caagagtccc cagattctgc tgcaaatcaa ggaagtttgt gtttgccttg
    21901 agttcggggt agttctcggc tactgcgaaa agggatctca gtgttcccgt cagggcgttc
    21961 tccgcctggg cgcgttcagt caccgacccc gcgcttaccg cggcagagcg ggccgcagtc
    22021 actttctcga agagttctcg ttcgtgggtc gcataacctt ttactgtctc cacaaggttt
    22081 ccgacgaggt ccgttcttcg cttcaactgc acatcgatac cactccagcc ttcatcaaca
    22141 aggttgcggg aacggatcat tgtgttgtaa gtcctcatca cgaacagacc taataccgcg
    22201 acaataccaa gaacaatcca aagggccgtc attttatctt cacctccggc gaatttttta
    22261 ctgattttga tactataccc caaaacagcc aagaccggac ctgggtcaaa taacgggctt
    22321 gacttctata tgaatggtga tatgctattg ataaccgata tcaatagagg aggcgatcaa
    22381 catgcccggt tgggatggga gaggcccatg gggcggaaca ggtcccggaa ccggctgggg
    22441 acttggtccg tgtggagcag gggcaatgcg cggccgtggt ttccgaaggg ctggccggtt
    22501 cgggggcggt tttggatacg gtagaggttt cggccccgga tggggtgccc cctggggagc
    22561 ggccccatac gggggatatc cggcaccctg gcagaatgtg gaccccgccg acgaaaaggc
    22621 agcactcgag gatgaactcg gggcccttga ggcgcaaatg aaggaagtca gggaccgtct
    22681 cgacaggatc gagaaagaat cctgatccgc tactgccaac tcttttctcc aggacccggt
    22741 tctgatgaac cgggtccccc tttttgggga tttatgtcat cattgagatc atcagttttt
    22801 caggaggagt gatttgatga agatcgctgt agcttttgaa aacgggaacg tatgccccca
    22861 tttcgggcac gcgccctttt atcgtatctt cgatgtgtcc ggggaatcaa taacaggaag
    22921 cacggatcat cagagtcccg gccacgctcc cggagttctt ccaaagtggc tgtcggaact
    22981 caaagccgat gtgattattg cgggtgggat gggtccccgt gctgaggagc tgtttgtttc
    23041 cttcggcata aagcccgtca caggcgtttc gggacccgcg gaagaggccg tcagatcctt
    23101 catcgacgga acccttaccc agggaaagag ctcctgcgga cacgttacag gagaggccgg
    23161 ggaccatcac cactaatgcc agtaaaacct ttcacgatag ctgtagcgag cggcaagggc
    23221 ggaacgggga agagttgcgt ggccgcgtcc ctgatggtcg ccactccggg acttctcggg
    23281 gcagacgcgg atgtcgaaga accgaatctt tcgcttctgc ttggggcagt ctccatatcc
    23341 tcatcaatgg tggagacggt cctccccgta gttgccgaga gaatttgcaa ccgttgcggc
    23401 aagtgcattg acgcctgtcg tttcggcgcg cttaacatgt tcggtgactc tttccccacg
    23461 ccaaatgacc tgtgtcacgg gtgcggtgtc tgttccatgg tctgcccggt taatgccatt
    23521 tcggagaaag gcagacccat aggcgaaatc cgcatgaacg tcaaagatga tctcaaaatc
    23581 tacgaggggc gcctcatggt agggatgcca aacccgatcc ccgtgatcca tgccaccatc
    23641 gaggccgcca tggcggagag aaaaaacctt gttatcgact gccccccagg gacctcctgt
    23701 cccatggtcg cggctgtaag gagcgccgac gtggtgatcc ttgtgacaga accgaccccc
    23761 tttggagcag cagacctgga ccttgccctt caggtagtca gcaaccttga aaaacccgcc
    23821 cttgtggtca taaacagggc tgacctgggc gaagcggaca tagactcact ggccgggaaa
    23881 tacaatgtcc ccgtggcagc ccgacttcct ttctcccgtt ccgtggcgga atcctacgcc
    23941 agggggaacc ccccggcctc gatcgatcca gagtggaaaa aagcaatgat cggcatacga
    24001 gaaatatctg aaaggatgtt caggaattga agccgaaaga aatcactgta gtcagcggaa
    24061 aaggcggaac cggtaaaacc tcgctaacgg cgaccctggc atctttttct cctcagaaga
    24121 ccgtcctgtg cgaggctgac gtagacgctc ccgatctcga aatcctcctg aatccgaccc
    24181 ttgaagagga acaccctttc atgggcatgc agacagcaac cgtcgacggg gacagatgca
    24241 tcggctgcgg cagatgtgtc gatgtctgcc gattcggggc catcgggatg acatcccaaa
    24301 aggctgtgat cgacaagact ttttgtgagg gctgctcagc ctgcgccctg gtatgccctc
    24361 agaaatgcat cagcatggat gacacaagac agggaacgtg gttcagggga cagaccgact
    24421 tcggagacat ggtgcatgcg aggctcaatc cgggagggga gaattcgggc atgcttgttc
    24481 agcttgtccg ccgggaggcc atgaaaacat ccgagagaac cggtgcaggc attattctca
    24541 ccgacgggcc tccgggaatt gcatgtccgg ccatctccgc ggtcaccgga gccgacatag
    24601 cactggtggt aacagagcct acgatgtcgg gcaaacacga catgctcagg atcgccggcc
    24661 tctgtgggcg cctcggcacg aaagtggcgg tcattctcaa caaggcggac ctcagcggtg
    24721 agggagcaaa aaaaatagaa gaggcctgcc tggagaacga cctgcctctt ctgacatcca
    24781 ttccttttct tatcgacgtg gtccatgccg tatcgcaggc acgaatcccc tcgaaggaac
    24841 tcgaagatta tgtcaaaccc gtctgggact ttctgatgga gatgtaacca gggatcccag
    24901 agcggacatt attttttctg ccatctcaac cggctggttc cccccggcgt aatcctccag
    24961 cctccccgca tcgctcagca gagacatgtt tctgtctatg gggaggctgg caagtactgg
    25021 aatatcccac cttgaacaaa tctcaccggt gtggcttgga ccgaagatct cccacttctc
    25081 cccacagtgc ggacacaggg cgtgactcat gttctcgacg acacccacaa gcgggacatt
    25141 catcattctg gtcatatgca tcatcttctc gactattttc gttgaaagat cctgtggcgt
    25201 cgtgaccatg actattccgt caagggctat ggtctgcatg accgtcaaag gcgcgtcaga
    25261 agttccggga ggaaggtcta tgacaagcat ttccgagtcg ccccagttgg tatcgttcca
    25321 gaactggctt atgacgttgc cgatcagcgg gcctctccac acaaccggcc tggacgtatc
    25381 ctccataaag agattcatcg aaattatcct tatccccaat gaaggtgatg ccaagggctc
    25441 gatcccttcg ggcgtgcctt tgggcattcc ggaaactccc atcatggaag gtatcgatgg
    25501 gccggtaatg tcggcatcaa gcagggcaac cctgttcccg agacgggcaa gcccgatggc
    25561 gaggagggcc gatacgctgc tcttgcctac tccccccttg ccacttccaa cggcaattat
    25621 gtgtttgaaa ccgcccctgg tggggacagg catggaacat gtcgcaggat cctggttgca
    25681 ggagccctga ctctggcacc cttcacattt gctatcgctc aatacaattc cacctcttcc
    25741 taaccgagag gtatctcctt gagagaacct ttcctgaatt tttccagtgc ctccaaacaa
    25801 gtgcacccac tggcctcaaa ggcacgaatt cccgaagcct tgagagccat tgtagcattg
    25861 ggaccaagcc ttggggcaat aagagtcttt gcgcccttcg cggctatgct ctgcacagcc
    25921 attccccctg ctccgtgggc agcctgggtg gcatcatttg acatagattc ctccaggttg
    25981 ccgtcctgat ccaaaatcag aaaaaaggga caccttccga aacgctccgc aaccatactg
    26041 tccatggtcg gaccttctga cgctatggct atcttcatct agaacctcca ttgtgataat
    26101 tgttatcaac agcagaaaat actatagtcc tccgacagga gcgagtcaat ccactctgtt
    26161 caccgtgaaa tgggaaaaac cagaaccaaa ggcgtaaacg gtgaacggga ggggcggaaa
    26221 ggcgaggaga ctggagacgg gaggcaggag acaggaaagc aaaaccaact gcgtaaacgg
    26281 tcaacggtca acggtgaacg gaaaaggcag aagggcctgt cattctgagg accaacggga
    26341 ggaagaacgt ctaacgtctt acggcttttt tgcccttcct gtttcctgtc tcctgtttcc
    26401 tgtctcctgt ttcctgtttc ctgtttcctg tctcctgatg cctcctgact ccctgatctt
    26461 ttattgatat tcactcttga caaaaagttt gggcatgcta acatttccga agacaaaaaa
    26521 ccaggagggt tcttatgcca accacgcttt gctccatgaa aaatggacag acagcaaaaa
    26581 tagtaatgct accgccgggc atgagcggcc gcaggcttga ggctttggga ttaaggcccg
    26641 ggaaagctgt caccaaggta tccggcatgc ccttcagcgg tccggtgaca ttaattcttg
    26701 acggccggca gatagctatt ggccacggaa tatgctgcaa ggtcaccgtg gagctttttt
    26761 cagcgggggg taaccaggga tcatgaagtt ctgttcccat tgcccccagg gaggatgtct
    26821 tcgcccatgc agcaacccgg accaggaaat gtccggactg aaaccacgca aggttcttct
    26881 ggttggcaat cccaatgtag gcaagagcgc actcttttcc aggttgaccg gagttcacgc
    26941 tgtttcatca aattaccccg gcacgaccgt aggtttcact gaggggaacc ttttcatcaa
    27001 gggggagaga ttccgtctta tcgatgttcc gggggcctac acgatagacc cgaccaacga
    27061 ggccgaggaa gtggcccggc ggattcttga agaggaagcc gacatcgtgg tgataaccat
    27121 cgacgccacg tcactggaaa gaaatctcaa cctggccctg caggtacttg agcttggaca
    27181 tccctccgtt gttgccctga acatgttcga cgaggcacgc cacgagggag tcaatataaa
    27241 tgtcgaggcc cttgagaagg aactcggcgt tccggttatc cccaccgttg ctgtcagcgg
    27301 ccagggaatc agtgatcttg tttcgcgtct cgaggaagcc agagtcgcaa aaaacagcgt
    27361 agtttcggga aaagaccgct gggccaggat aggcgagata gtgagttcgg ttcagactat
    27421 cgggcatcgc caccattcac tcagggatcg tatagaggat ttttcggttc acccgggctg
    27481 gggagcagtt ctcggagtcc tggcgatact tggcagtttc ctggccgtaa gagccctcgg
    27541 tgaagggctt atagattttg tcctggatcc gatctttgaa aatctgttca tgcccctttt
    27601 cgcatggatc ggctcactcc tcgggggcga ggggtctttt cttttcagga cacttattgg
    27661 tatgacaata gacggagaga tagatccgga aatgagtttc ggactgctta ccacgggcct
    27721 ttatgtccct ctcgtaatgg tcctgcccta tgtcgtggcc ttctacggag tcctgagttt
    27781 ccttgaggat gtgggctacc ttccaagatt cgctgttctt ttcgacgcac ttatgcacag
    27841 gataggtctt cacgggttcg ccatagtccc gacacttctc gggctcggat gcaacgtgcc
    27901 gggcataatg gcaaccagga tactggattc gcggagggaa cgtttcatcg ccgctaccct
    27961 gatatcggta gcaataccct gcgccgggct ccaggccatg ataatcgggg cccttggcga
    28021 tcttggcggc cgctacgtcc tggctgtcta tgggaccctt ttcctttcat gggttattct
    28081 tggaagcatt cttcacagat tccttcccgg tttcagcccc gaactggtca tcgagatccc
    28141 cccttacaga cttccttcct tttcgggaat agggttcaaa ctgtggctca gggtaaaggg
    28201 tttccttatc gaagcaattc cactggtcct ggttggggtt ttcgttgcca atctactgtc
    28261 gacatcaacc gtcatgcaca cagtgtcagc cttcttgtcc cctctcttca ggggagttct
    28321 gggccttccg ccggagaccg cggggccgat tcttctgggc tttctccgca aggatatcgc
    28381 tgttggaatg ctgatcccga tgaggctcga cccggggcag atggttgtcg ccgtcgtgac
    28441 ccttgcaatg acttttccat gcattgctac cttcattgtt ctgtggcgcg agctgggtct
    28501 gcgcgacatg cttctttcga tcggcattat gatcctcgcg gccctggttg ccggaggcct
    28561 gctgaacgct cttttcgctc tggcctagaa cgagtttcat gccaggggga aagctgcccg
    28621 ttctccatcc gggagggttt ccagcctcca gccggtatcg aatatcctcg caaggagatc
    28681 tccgtcaacg agccctggag accggggctc gaagagcacg ccgtctttga ggaaaaatat
    28741 cctgtcggca aaacgcagag cgtagttgat gtcgtgaagt gcgacaataa tgccttttcc
    28801 cttttccctt agttccccca gcagccggaa aacacccatc gcgtgccggg gatccagagc
    28861 gctgacgggt tcgtccagaa gcaggaccga agtatcctgg gccagggccc ttgctatggt
    28921 cactctctgt gcctctcctc ccgacaggtc ggtgaccagc atctcctcaa aagatgaaag
    28981 atcaactttt tccagcgccg accttgccat atcggcatcc gctggagaaa gcctcgcaaa
    29041 cctgcccgaa gccgggaacc tgcccatcag aactgtctcc attactgtga aaggcatgaa
    29101 aaaacctgtc tgctgaggca ctacggcgac ccttttcgcc acctcgcgcc gaggaagccc
    29161 cgccagatca tcccccagca gaaatacaga gccgcgatcc ttgcggccaa tgccccccgc
    29221 cgcagccagg agagtgctct tcccgcttcc gttgggaccc aggagggcta tgatctcccc
    29281 agcgttaaga tcaagatcga tgctctgcag gacctcccgt gaaccaaggg ttacacccag
    29341 tccgcgcagg gaaagcaggg ttttcatctt tgcctccaga gaagaaaaca gaatatcggc
    29401 ccgcctatca gggatgttat gacacctacc ggcagttcgc cgagagttcg ggccgcagaa
    29461 tcagccagca aaaggagaac tgcacccccc gtaacgctca ggggaacaag tcttgcgtgg
    29521 gacggcccta tcaggagcct gcagaggtga ggcacgacca ggcctacgaa tccgattatg
    29581 ccgcttatgg atacagccac agccgtcgcc agagaacagc cgaaaagcaa gagacagacc
    29641 gtcttgcggt catccactcc gagggatctg gggtttgccc cggaagaaat tacatccaga
    29701 tcccttgccc agaaaagacc aagaacaaat acaagaaggg cggacaaaaa actccacccc
    29761 acatcacccc aggatgaaag agagaaactc cccataagcc aaagcactat cgctcctacc
    29821 tggtctccgg aaagagcctt caccagagtg atacaggctc cgagtatgct gccaactatc
    29881 acaccggcaa ggataagtct tgcgggggaa gccgattccc tgcctccatg ccccaggatc
    29941 cagaccagac ccagggcggc cacaccaccg acgaaagcca gaggggatac gaaaggcatt
    30001 ccgagggtga tggcgatcga ggccccgaga gctgcccctg tagcaacacc aagggtgtaa
    30061 ggttccgcaa gaggattcag cagaactccc tggaaaacta ctccgctccc cgcgagcacc
    30121 gctccaacgg cacaggctgt cagtgcccgt ggaagcctta tcgaaaggat gatcaccgac
    30181 ctgggatctg ttccggggat cctccccaga ataacccgga cgatctcccg gatcgttatc
    30241 ggcacctccc ctgtggatac gacccagaca agggaagcga aaagaaaaaa aacaaggacg
    30301 gaaaaaagga tccggaacga actcctgttc atgagcgggt cattccccgg ctgccgcgga
    30361 tagaagtgat atctccttac ggagcgtttc cgccctcagg aggaccttct cctgggaaat
    30421 accaagtttt tcggcatacc agaccgcccc gccggctccc gcaccgtcct ggaccgggcc
    30481 tgcctccatg cccgtcacat ccacaccgat cctgtcggct aggggcatga gacccggggc
    30541 agaaaaaccc gtagtgacga gtctgaatct tcccctgtaa ccaaggtctc tcgaaagggc
    30601 agcaacagcc agcatccgga tgtctccggc aagcactatc tcggccctgt ctttggctcc
    30661 cgccaatatt ccagccgcaa ttatctggaa agggtccccc accgctgtca cggcttcaaa
    30721 cccccggccg gaaaagcagc cccggctaaa accgggaatc tcttcttgtg atccctgagg
    30781 atcatccaga ccgagagaaa caatgagggc tgaggcggtc tcatgcccac cgggaacaga
    30841 ctcggctatg acaagatcct cccccatccg cgacaggagt cttccaatgc tgaagccgca
    30901 gtcccatagc ctttcggagt ctttgacagc ggccctttct cccggctttc ccccggggac
    30961 agccccggtc tccatgtatg gagaccgggg aggagtaaag gaacccgcac gaactattat
    31021 catgggaaaa tcggatacaa gcctggcggc ccttgtgagc atcgaaggca aaaccggaaa
    31081 atctcccgga aggtccctga ctttttccgg caccggaaaa ccccaccaga taaaatccgc
    31141 ctcttcgtgg gaggcaaggc gacacgaagg attgtctcca gccgaggtgt cacctgtcag
    31201 gaggaggaac aatttttctt ctccctttct gtcaccctgg aagcggcatc gagatgttta
    31261 caccgtcccc ttgcccggaa agatgggcaa ctgcatgaag agtgcagcac atccaccgcg
    31321 taatcagaac ttccgtgggc aataaagatc gatcctgaca cacgctcaag cctttcttca
    31381 ggaaaagatg aagcggcggg aaccttctca ccgtcaagag cccgatttga aagagcatcg
    31441 gccctgctgt tccgttctct cgggatccat tccagaacag ccccggtctc atcgaccagc
    31501 gttctcgccc tgcccgcgaa acctttcagc ctttcttccc ggaccttcca gtctcctttg
    31561 acctgggaga tcaccagcct gctgtcaccg cgcacgatcg tgcccctgag acctctcttt
    31621 tttatctcct ccagaaggat tatcagggcc tggtattcgg cctcgttatt cgtccccacg
    31681 ccaagagccc gacactcttc ccagaggatc tcaccgtctt ctccaataat gcaggctccg
    31741 gcagatgatt cgcccgggtt cccgcgggaa gctccgtcaa aaaaagctac agccatggtc
    31801 aacaattctc ccctttaatg atcaccggtt cactacagtc tgccccaatc ggcctccaga
    31861 cccaggcccg ccccctgggg aattatcttc catatccatc ggctgtcagg gtgataaggg
    31921 agctggaagg agaagattct ctctatcttt ccctggtcac tgctgctggc gtagccgacg
    31981 tgaagccaac ccccttcaag aacgttccag ataccgatct gcccggaggc aagagagctc
    32041 cactgcacct gggttgggtt gaactgctgg tagtccgatt ttgtcaccca taccattctg
    32101 ccagtggtgt tacggagatc gagcacgcga cttcctccaa gggcaaaaac cggcatggca
    32161 accaacaaac agaagaaaag agcggcgcac agggcaaaag aattcctctt catcagatca
    32221 gctccctttt cataaaatgc tcccctccag cagggcagac tttccaaagg agattatacc
    32281 ctctgcc
//
LOCUS       DAOTIQ010000013        29968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1734_length_29968_cov_7.636078, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000013 DAOTIQ010000000
VERSION     DAOTIQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 29968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29968
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_29968_cov_7.636078"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..848)
                     /locus_tag="P5541_03225"
     CDS             complement(90..848)
                     /locus_tag="P5541_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71019.1"
                     /translation="MILQVKGISFRYNSRPVLEEVSFGLRNNELLAILGPNGVGKTTL
                     LKCLNGILRPSDGAIMVEERELLDMKQMEIARDLAYVAQQGQGGRLTAFDAILLGRRP
                     HIAWKVGKEDICKVDAIIRALGLEHLSLRHLDEMSGGELQKVSIARALVQEPRILLLD
                     EPTSSLDLKNQQEILATIRHIVTGHGMAAIMTMHDINSALRFADKVIFMKGGEIYAAT
                     DVEGITPEMVCEVYGIPVAIEIFRGFPVVVPVGS"
     gene            complement(845..1915)
                     /locus_tag="P5541_03230"
     CDS             complement(845..1915)
                     /locus_tag="P5541_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV71020.1"
                     /translation="MHLEEGTVPAGYAKYIQAKVLLLLLGLVLLLALAVFSISTGSVS
                     YDPVEVLRTLFMDHVSRQLDVVVFRIRLPQVIAAVIAGAGLSVAGVVMQSVLGNPLGS
                     PFTLGISQAAAFGAAFSVMVLGSGFIQSSASDAITVVRPGMTTLSAFAAAMIATGVVI
                     LIARLRRGSPEVMILAGVAIGSLCTAATMFLQYFADDVQLAAMVFWTFGDLSRAGWRE
                     IGVISSVTGLCFFYFVVNSWNYNAIDAGDETAASLGVNVRMVRFFGMSLASLVTAVII
                     SFVGVIGFVGLVCPHMVRRLLGDDNRFLIPGSCIMGSMLLLAADTAARVMMAPNVLPV
                     AILTSFVGAPMFLYLLVRGYRR"
     gene            complement(1921..3063)
                     /locus_tag="P5541_03235"
     CDS             complement(1921..3063)
                     /locus_tag="P5541_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRV71021.1"
                     /translation="MSQRVSWTRWIFLVMAVLFVVIVSSVSCVSAEVTVKDAWERDVV
                     IPEKVTKVICSGPGCLRYLVYLQAQDLAVAVDDMEKSRNMFESRPYYIAHPELKDRPL
                     FGEFRGHDNPELIVALDPAPQVIFKTFGNMGHDPVELQKKTGIPVIVLDHGQLGVGKK
                     KMDETLLMMGRVIGKEKRAKEVIAFFDEAIEDLRKRTADIPEDKRPTCYIGGVAYKGP
                     HGITSTEPAYPPFLFVNAVNIAADPSKAKSDQLAQTTFSVESLVSMDPDKIFVDLSTL
                     QGGDEVNGLYQIRDQAAFQVLSAVENGEIYGVLPYNWYSQNHGSILADAYFIGKVLYP
                     DRFEDVDPVKKADEIYTFLVGKPVYEEMDRSFGSTVFKQIELSKVE"
     gene            complement(3148..3915)
                     /locus_tag="P5541_03240"
     CDS             complement(3148..3915)
                     /locus_tag="P5541_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRV71022.1"
                     /translation="MIGAIAGDIIGSAYERYPVKTTDFELFLPSSRFTDDTVLTVAVA
                     DSLLGRGSYDDCLRRYAANYPDAGFSRATLGRLLSPPGGSTDSLGNGAAMRVSPVGRA
                     FGDEETIIREAEKISLPTHGHPLGVIWAQAVALGVFWARKGMGNAEMRKRVEGFTGVS
                     LDRTIEEIRPGYRFDATAPGSVPESFIAFLEASSYEEAIRLAVSLGGDADTQACIAGA
                     LAEARFGGVPEGIESRARERLPSQFIEVLDEFETRYH"
     gene            3984..4364
                     /gene="queD"
                     /locus_tag="P5541_03245"
     CDS             3984..4364
                     /gene="queD"
                     /locus_tag="P5541_03245"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRV71023.1"
                     /translation="MFLKKTFTFDAAHRLDHYHGKCEALHGHTYRLQVTLQGKPDSED
                     MVMDFVQLKDLVKERVISRLDHAFLNDIITQPTAENIALWIWQQIDCEIQKEDCSLFS
                     VEVWETATSSVLVHREDIIPRKGA"
     gene            4369..5109
                     /locus_tag="P5541_03250"
     CDS             4369..5109
                     /locus_tag="P5541_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRV71024.1"
                     /translation="MKVIPVLDIMDGSVVQGYRGERSGYRPISSSITTSPDPVENALS
                     LLAITGGSDIYAADLDAIQRKGENTEQLRNVVRETEASIWLDSGTGSLRTASAVLVSL
                     PGSKVVIGSETLDSEPDLRDITTHIPWDRRIFSLDIQSGEILTGKSSPLRGLGIPEAL
                     SFLEDSGWSEVIILSLDSVGSGDGAALDLIAGMVQKFPDLSIYAAGGCRSPKDLENLA
                     ACGAEGTLVATALHRQWIKAADITEFCS"
     gene            5106..5846
                     /locus_tag="P5541_03255"
     CDS             5106..5846
                     /locus_tag="P5541_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HRV71025.1"
                     /translation="MNPLFPENTSLLATVLSAEETLVAVAGGAGVIDVKNPSEGALGA
                     PAPSVIAAVRGATPDHIHVSAALGDMPFLPGTAALAAVGAASAGASIVKVGLYGPSNP
                     EEAFLLLEVTAGALRNSFPETVLVAGAYADASRFGGIDPLVVPECAARAGAAGCLLDT
                     FDKSPGHTLPDILPLDAIEFFIRSCRENGLFSALAGSLGTEETALLLPLRPDYLGYRG
                     ALCENGRTGRLSGEKVRQISLAITEGSR"
     gene            complement(5827..6438)
                     /locus_tag="P5541_03260"
     CDS             complement(5827..6438)
                     /locus_tag="P5541_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase"
                     /protein_id="HRV71026.1"
                     /translation="MGPGADIVVKFGGSLLSRSNLRDICCRLDSSLREVNAIIAPGGG
                     IIADEIRRLDSLHYLGPESSHWMAVLAMDQNGLMLADLMPRGIPVRSGEEAAAAWEKG
                     LLPVFLPGEKMREEDPLPKNWDVTSDSIACWVAVSLGAKKILFLKDSIPGCSMTAKSL
                     SSHGWLDRAFPDQFGFFHGEAWIGNGRFPEDAVNGPGIIEILR"
     gene            complement(6450..7484)
                     /locus_tag="P5541_03265"
     CDS             complement(6450..7484)
                     /locus_tag="P5541_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRV71027.1"
                     /translation="MTWFSDRTVIGWDIGGVQTKCAALFPDGSPAGKGSRSFEVWKGP
                     NGLDEVLKNLGSRFPGAERAVMAVTMTAELCDCFSTKKEGVLFVLESFGRSFPSSEVW
                     CISGRGEWKTLEEAFRTPGDFAATNWRAAAAWLARQFGSGLWVDTGSTTTDIIPLIGG
                     VPAALGNTDTERLTQGELVYTGVVRSNPDTLADRVPVRGAMCRVADERFSSMGDCYIV
                     LGDMDGSAYSAPTCDGRPADVQHAVSRLARLACADSDLISAHEARMMAVYLREKQIGA
                     ITEAMLQVCSRFSESLPVIPTGAGKFLVSEAARRIGLPEKGLSGVIPDGYETHLPAYA
                     AAAGFLSMSK"
     gene            complement(7468..8574)
                     /locus_tag="P5541_03270"
     CDS             complement(7468..8574)
                     /locus_tag="P5541_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRV71028.1"
                     /translation="MSSLKSREPGSLVIFEYVSGGGPVSGSASAGLAGEGLLMAGALF
                     RAFSELDGISASLLLRKDISFPMVEKNGRVIPVNPGEGVDRFISECREREFAVLIAPE
                     TDGLSGVLAGMAAGTGVRLLGPDPEAVALSSNKALLMKALETNGVPVPRWCVAREVRA
                     VQDAARETGFPVAAKPLQGTSCEGSAVFCESTEIDLFFAAPGNERAVLLVQPLLRGDA
                     MSLSLVVSRSGRARLLAVNTQDVVIPSEAGSPGGWRKFIYRGGIAGVEDAFFSRRGKW
                     GCPVLEDLAQRVVSSIPGMMGFVGVDIVMTDEGPVVIEVNPRLTTPLAVAGAKAPWNP
                     GKILLDACMSDSLPFDLKVPRVSFRKDDPLDLVF"
     gene            complement(8577..9458)
                     /locus_tag="P5541_03275"
     CDS             complement(8577..9458)
                     /locus_tag="P5541_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HRV71029.1"
                     /translation="MSRAEHVLWAAQIACQLEITALKPGNVTWDRPFSDVAAEQFLAS
                     SAAMGRSFSDLGRKRTGELILQAVMDTREVAGCNTNLGIALLLFPLAKAAIGGPTGEL
                     RERLAMELRLLTEKDTRDVYQAIRIASPGGLGGSDRYDVKEVPPSKPFLEAMAEARER
                     DSVAREYVTDFEITFNTGLPAFDDSAKAGLSLRDAVTQTFLTILSRFPDTLIARKRGN
                     EDASAVSVEASRVLGSGAAGSEKWRSSVLEFDSSLRDLANSFNPGTTADLTVAVLFCE
                     VLKALEEGTLPEMLRKW"
     gene            complement(9455..10363)
                     /locus_tag="P5541_03280"
     CDS             complement(9455..10363)
                     /locus_tag="P5541_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HRV71030.1"
                     /translation="MGTLRAAILGRGDGWHVRSLHDSLARRGIEAPCLPSTRIVSRCG
                     PGSSPFIETGGVDLESLDMLFVRSLPGGSLEEVIYRVDALHRLESLGVRVINSASVIE
                     RTVDKLFTLCLLERAGLPVPATVTCERFDQAMEAFEELGGDVVVKPLFGSEGRGMVRV
                     SDPDMAYRVFSALEQQHYIFYLQEFLPHANRDYRLFVVAGNVIGAMQRTGRNWKSNVA
                     QGAQVAPAEPDIVMESLALDAAKVVDAEYLGVDILRTVDGRNMLLELNGIPGWRGFQK
                     ATGIDAGNVLVDHILGETSRKAVRTG"
     gene            complement(10351..11298)
                     /gene="mch"
                     /locus_tag="P5541_03285"
     CDS             complement(10351..11298)
                     /gene="mch"
                     /locus_tag="P5541_03285"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HRV71031.1"
                     /translation="MKPLNEKAAELVRQMAAKADELDVRVVQTVGGAMVIDSGVRSPG
                     SLEAGRLYAEICMSGLGKVSFVHYHEDGLSMPGLCVAVIRPSLACMASQYAGWQVSSG
                     SYFAMVSGPGRILRGREELIREIGYSEKSDVAVLTLESEKLPPEEVVESLSKECGVSP
                     SSLLMVVARTNSITASTQVVARVVETGMHKMHQCGFDIGTVKSGWGTAPLAPVGRNSL
                     EAMGWTNDSVLYGGDVWYAVETDDALIEAVIEKLPSSASSDYGAPFAELFRKYEGDFY
                     KIDPLLFSPARVTINNISTGRVFRAGSPSTQVLRKEWGL"
     gene            complement(11285..12118)
                     /locus_tag="P5541_03290"
     CDS             complement(11285..12118)
                     /locus_tag="P5541_03290"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HRV71032.1"
                     /translation="MAMNENSSVFLECFIKETIPVEAPCLTPGSVLGRSEKEIASLPV
                     LVGNRNYSLGDIFRIRRSGDDSLVVSGNLGHVKRIGEKMDKGILKVRGFPGMHLGEEM
                     SGGQLLVDGDIPDWCLCGMSGGLVHVTGSAGNRTAAALPGESRGMKGGVVVIMGNTGS
                     RTGDAMRRGLIAVGGSAGEFAGSRMVAGTMVVFGRLARRAGGGMKRGTIIALGGADGI
                     LPSFVRACRYRPSFVPLYMGYLAKLGLPVDINTVAGDFVRWTGDCTSLGKGEILEHEA
                     A"
     gene            complement(12133..13014)
                     /gene="fhcD"
                     /locus_tag="P5541_03295"
     CDS             complement(12133..13014)
                     /gene="fhcD"
                     /locus_tag="P5541_03295"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HRV71033.1"
                     /translation="MRINGVEIVDTFAEAFGMWGARFCVTAENRRWLDAAARSVAGFA
                     TSVIGCGCEAGIERCLEPSETPDGRPGVHVLLFTPSKKNLGKQLVSRIGQAVMTCPTT
                     ACFDALEGSEKVPVGAGLRYFGDTFQASKMLEGKRYWRIPVMEGEFLVSDSFGIQKAV
                     GGGNFLIIGRDAPSVLRAAEAAVDAVEDLPGVILPFPGGVVRSGSQVGSRYSFLPAST
                     NVAYCPTLRGLVESALPEDANSVLEIVIDGLDDDSVAEAMRTGIRAACIEGILQITAG
                     NYGGSLGDHLFRLHEIF"
     gene            complement(13030..13998)
                     /locus_tag="P5541_03300"
     CDS             complement(13030..13998)
                     /locus_tag="P5541_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HRV71034.1"
                     /translation="MRRCTVSSHSRLHLGFLDLHGGLGRMFGSIGVGLESPCMNLEAV
                     ESPVLEAGGDDSDRTRSFAEKFYQTTGIPEETRSRLLLRSGLPPHAGLGSGTQLALTV
                     GKALSILHDMPLTTREIASMMGRGRRSGAGIALFDDGGLVIDGGKKTEGKDELPPLLY
                     RRRIPENWFFVVAIPGSGTGMSGEREVEAFRDLPGPSPEVSGAICRETLMRLLPALEE
                     EDLGAFGEAVTSIQRMVGDCFAPAQSGRFASPLSERIIRSMKELGAAGVGQSSWGPAV
                     YGIVDGIEVAEGLAAEVARIFKDQEDLEVTVSAAARKGAVWNVEPI"
     gene            complement(13995..14546)
                     /locus_tag="P5541_03305"
     CDS             complement(13995..14546)
                     /locus_tag="P5541_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HRV71035.1"
                     /translation="MIIEVILSTRNGNGEDNFAPMGMERIDETTVLIRPFRDSGTWAN
                     LNEKGFAVASITDSILPFVHAVARDNMPGHFPAYMVPCGVIDDPCGWLELELKRISIE
                     GDRGHCLFEVAYEESRRPFTGFNRSRCAILEALVAATRIHVRGKEPFLAEYRRAVELT
                     ERTGGEEERLALRLVGELTGGIG"
     gene            complement(14543..15871)
                     /locus_tag="P5541_03310"
     CDS             complement(14543..15871)
                     /locus_tag="P5541_03310"
                     /inference="COORDINATES: protein motif:HMM:NF040121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HRV71036.1"
                     /translation="MAHYLFLTGQLAAPSLRSILEGMEPPFEYDVHVMPITVAALAEC
                     GWMSRQLPEVAGYDAVYIPGLCQGSISMLQEAAGDTPVKRGPDHLKDLPGFFNLEGED
                     VSLEGHDITILAEIVDAHLLHDGELVRSASRYREDGADVIDLGGPVSGEFPGVAGKVR
                     LLRKEGFRVSVDTFDGKSLREAAKAGAVLLLSVNSSNIESVLDLGCRVVVIPDFRDRS
                     LNSLERNIEILESSGISYLADPVLDPFPFGLIESLERYIIFRRRYPEIPMLMGIGNQT
                     ELMEADSSGVTAVMAAICTELSINSVLTTSVVSWATGAVAEFDRARRLMFWARDSRVL
                     PKHAKAGLVALRDLPHQRFLPEELQEMKTAVRDKNYRIFLSTTGIVAFNSDSLATGST
                     AREIWEQINIDDAAHAFYIGRELEKAETALKLGKRYVQDQPLDWGYISRP"
     gene            complement(15850..16386)
                     /locus_tag="P5541_03315"
     CDS             complement(15850..16386)
                     /locus_tag="P5541_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRV71037.1"
                     /translation="MKDGRSPGTAWCLTGAGHFLKGSVELALRLGNVTFFLSRAGEEV
                     TTMYGLMDIILKSGVRVVYDRTASSPSCGEMALGRFDRLVIAPATANTVAKCVNGIAD
                     TLPANLFAQAGKALVPSFFLPTDLESGVVSADPSGREFPVRSRKVDLENAERLSSFPL
                     VRVAATLEQLEKWLTTSS"
     gene            complement(16383..17849)
                     /gene="mptA"
                     /locus_tag="P5541_03320"
     CDS             complement(16383..17849)
                     /gene="mptA"
                     /locus_tag="P5541_03320"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HRV71038.1"
                     /translation="MPIEERSLHTVYLLLGANLGDRQSNILQALQYLQGRAFIEEVSS
                     FYETEPEGMNGQPLFLNLACRLRTDMEPHKMLRFVKSIELRMGRADASRNLPRPIDID
                     ILLYDSISLRTDELTIPHSRLAERAFALVPLAEISPEIVPPGFSESIGDLSGKADLSG
                     IRKVEKSLRFKLERDVQQSSPSFPLVLSRVGVTNLKRIIRMTDHGRESLFYADMDLFA
                     YLDQKQAGVHMSRFSDVLERLTEEISLEPSSTIEGMAERLARQIAATQRTERAEVFIR
                     AKYPLKKLTPLSGRSVEEIYTFIGIASCSGEKVKTVTGVEVEGMTVCPCAQDMVRSHS
                     KKLLVEDGFSDSEAERILGLIPLASHNQRGKGTLLIGGNWPVRAEHLVHVLEASMSSE
                     TYELLKRSDEFFIVNKAHQNPMFTEDVVREVFRNLIDIYPDLPDETFVMVRQENLESI
                     HQHNAFAERSGTMGAIREELKNDKPSIERITLQEWLQS"
     gene            complement(17840..19519)
                     /locus_tag="P5541_03325"
     CDS             complement(17840..19519)
                     /locus_tag="P5541_03325"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HRV71039.1"
                     /translation="MSAKTVGIIGGRLYDPANGIEGEVRDVWIRDGSVVAPDEADRNG
                     AMIIDAAGMNVMPGGVDLHSHIAGAKVNHGRKMCPEEHAAHKRSRLEGGRSGAGFTVP
                     TPYMTGCMYAEMGYTTVMEAACAPLVARHSHEELEDMPFLDRGMLITMGNNEFLFDCI
                     AKGEMELARDFIAWTLKAARGYGIKVVNPGGVENWKSSRSNVGSWDQDVYGFGITPGK
                     ILETLTWAADSLGLPHGVHLHGLNLGTTESAEHTLRSVREASGRLHLTHLQFMSYGKD
                     KKGRFANRTQELADAVNANSNVTFDVGQIVFGPATTMTSDGPFQYNLGRMTGHKWFGG
                     DEENETGGGIVPIRYSRKNKVNALQWVTGLELFLLAKDPWRVFLTTDHPNAGPFFCYP
                     QVINLLMDRSYRMEVLETLPESVRKRTILGEIKREYSLGEIVTITRAAPARVLGLVKK
                     GHLGPGADGDVAIFHPADDGCRQPKCRSANGCKRKMDFPRWVIKGGEIIVKNGKAAGE
                     IHGKTLHVAPPFDASVEKVIREHFGKYYTVSFDNYPVQDCYLPSKEEVPCR"
     gene            complement(19516..20784)
                     /locus_tag="P5541_03330"
     CDS             complement(19516..20784)
                     /locus_tag="P5541_03330"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HRV71040.1"
                     /translation="MKNMDSVVCTFCGCLCDDIKVSSKDDGTIEIKGACANGKGHMEE
                     YDPSPVSPAFRGEPVSWDKAIGEAASILKNARSPLFYGLSSTASEAQRKCVALGDRIG
                     AVIDTTSSVCHGPTGIAMQTFGEPTCSLGEAMNRADFLLFWGCNPMIAHSRHFTRYSL
                     MPKGRFVPEGRKGRRAVLVDVRETPSAKAVDRFLRVKQGCDYEVLSALRALVQGKSME
                     AAEVGGISREELVSLAEEMKGCRYGVAFFGMGLTMTSGKDLNVGQYFNLVRDLNAHTR
                     FSVIAMRGHGNVAGADAVLTWQTGYPFAVSLAGGVPVYGPGEFTAVDMLARHEADAAL
                     IVASDPKAHFPAEAVAWLDAIPHIVVDPAFPLTARGATVYLPGAQYGVDAEGTYYRMD
                     GVPVRTRAFRERHRPTDEEIFDRLLEEVSR"
     gene            complement(20781..21131)
                     /locus_tag="P5541_03335"
     CDS             complement(20781..21131)
                     /locus_tag="P5541_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRV71041.1"
                     /translation="MAEKENSRNCVLITGRTQAQTKGMHLGKTGDFYANAVSVAVMHP
                     EMFKTMGVEVGCTLRLRSDSGEISVRSESDPGMPADMVFVPMGPVANRLVGPDTGGCG
                     MPSFKGIRVEVTPE"
     gene            complement(21124..22365)
                     /locus_tag="P5541_03340"
     CDS             complement(21124..22365)
                     /locus_tag="P5541_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRV71042.1"
                     /translation="MRPRFTRPSRSSTKIDRNLLIVAFSTRGLADLACGSGFRNITAV
                     DAFGDRDHPSSVENISTRRHFGLPFTDEAILKACEGRARGGIFVYGGGMENRPGLVEA
                     IARGRLIAGNSVHVLGKARDPSILGSLCREESIPFPEVLGPGEEGRAADEPDRSANEA
                     RWLRKRVLSGGGSGVSEYRGGRIESGEILQRRIPGRPVSVSFIAHRDGALITGFSEQL
                     TGTSFLGGKGYRWCGNITPLPARQEDATRFYEKASYYAEIISDRLGLRGFSGIDMILG
                     PDGSVAVIEVNPRYSGSMELFRPAGDKGELFSLHLAACRGERLRGKRPVFCGEGPFRA
                     KGIVYAVRDCAAPDTLAWSEAGRRDVPWTGDVFSPGDPVCTVFSSAPEGEECIGRLAE
                     EAGKVYSELVALCEGGNRENG"
     gene            complement(22319..23182)
                     /locus_tag="P5541_03345"
     CDS             complement(22319..23182)
                     /locus_tag="P5541_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HRV71043.1"
                     /translation="MKKILLQLDTDPQPSVFDAVTAYDGGADIILQHGSITPETAVPL
                     VHGVMFTRGGDSMKNSAVFVGGSDVAAGEAVLDAVKGAFFGPLRNSVMLDSNGCNTTA
                     AAAVAKLAGAVDLKGKKAVVLAGTGPVGLRAALFFAGEGAMVTLTSRKMDKAKAACAQ
                     LKERFGVSAEPAESGDEASVRKALEGAVVVLSTGAAGKMLLPESVWKGHATLKALGDL
                     NAVPPSGIENIKPHWDGKDIDGQLVFGPIAIGGLKMKVQRSCVARLFESNELVLDAAE
                     VYQAVKEFNKD"
     gene            complement(23219..23737)
                     /locus_tag="P5541_03350"
     CDS             complement(23219..23737)
                     /locus_tag="P5541_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HRV71044.1"
                     /translation="MLLVVNADNVTGEGLPYLIEGLMERGASSVHAIPAITKKGRSEF
                     VFLIDAPRSCLEELGAFLALELDTLGMRVFEPEHFPFTPVKHSVVRVGSRDRDDHYAE
                     VRIKILAGTMEDVVSCKAEYDDLEAALRKFNPGSAISFKSFKAAVELACMSGEPVNVC
                     GLVFSLRDGSSR"
     gene            complement(23814..25607)
                     /locus_tag="P5541_03355"
     CDS             complement(23814..25607)
                     /locus_tag="P5541_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71045.1"
                     /translation="MHKELYSAIAAANTFVENETSDRLEAITKGHGMLNLAVYSAANV
                     MVKEILQGRSTKLSDANMDNLPVDMIVEAGIKAAKDAGADSANAALIVAVFLNLAGTA
                     SRAGVPAGNRKLGSLARMKAGASRAGVQAIPTSKLTNKISGFPAVKAIYDAIEKGEIC
                     RFSGAVVPPFVAGGAIYGHSVLGEDMIYHDLAMKGTKVGVEAMFKAYQGVGINPNPWL
                     SAALAAAAILEIINPDGMIGQDYGEFFVQGTGYLVGKGAVQAAGLPEKLHMRGTGKEF
                     DTAALIGDLGMILKDVGAPTVVGMMTFNEMMACFQEGPMIGAGFGAGPVNPPLAHLVS
                     DCVVASMKLIESDGDVEAAADMIHDMKLNEWLDPEISAASGNMVAHKAEQVMRGPVTA
                     AVIKGTQGIRNNLQFRLAKFTWDGLQAGKSLEDICMEIDNQRKQKVEQRTGMMLGGMF
                     GKKITLEFTKLKGGARRNHPFAETYWGFDADIDAVVTVDGKEFKIEGLSHKVAPDVVL
                     NKKSEWTIPVTVASVAAQELMYVGCCTVNVVIPAAVAAVSGKMSAKDAGKCAEKGACI
                     TNAIPGAKESARSVANLAIRLMKDMEDVTEL"
     gene            complement(25867..26724)
                     /locus_tag="P5541_03360"
     CDS             complement(25867..26724)
                     /locus_tag="P5541_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HRV71046.1"
                     /translation="MKKVLFLNCFSGISGDMMLGLLLDLLGKDDIQPFLKGLALESYR
                     VDVSRSKKGGITGVDVKVRCDEKHSHRGLKEVLEIISKSDISPAAAKNAETAFRLLAE
                     AEGFVHGMPPESVHFHEVGAVDSIVDIVGSCVLMDVLAPAEVVSSPVNVGSGTVKCAH
                     GELPVPAPATMKLLEGIPVYSSGQAMERTTPTGAVLLKVFVDSFGTLPSGVVEKTGYG
                     IGDHESDLVNVLQGVLLEKKHFGAAHDHHDHDHHDHEHAHEHAHAHTHEHAHTHEPEI
                     KETCGTLKR"
     gene            complement(26721..27500)
                     /gene="larB"
                     /locus_tag="P5541_03365"
     CDS             complement(26721..27500)
                     /gene="larB"
                     /locus_tag="P5541_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRV71047.1"
                     /translation="MDERELRLLADKLRREEISVDSFVRSLKGLPFRDLGEVKLDTHR
                     ALRNGFPEIIYCPGKSPDQLKRIAGALSEHPGKALFSRMSAEQHEILLPILPDLEYLQ
                     GPRMGRLWPDRGESARGLMLVVTGGSCDVPVAEEAAVTAEIMGCNVRRLFDVGVAGLH
                     RLLAHLDMLLDADAIVAVAGMDGALPGVVAGIASCPVVAVPTSVGYGAALNGIGPLLT
                     MLNSCALGVSVVNIDNGIGAGYAAALMIRKKNSGDPEGGSR"
     gene            27766..28581
                     /locus_tag="P5541_03370"
     CDS             27766..28581
                     /locus_tag="P5541_03370"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRV71048.1"
                     /translation="MDKPGVVIRFFQEYPGTLVLLSGGVDSAVLALLACRGTGGTTRA
                     LSFRTPLVKKSETAMALEVASFLGIECHVHDVDVISDPRVRANCRDRCFGCRKILHAE
                     AKKFALSGGFSGIADGVQADEIHEPRPGLIAAEEDGIIHPLADAGLTKKEIREIAREA
                     GLPNSERPAAPCLATRFPPDIPLSAGWAEKIEAGEEILAAEGFSNSRIRWFPPGAASI
                     EIDEKDIPSFWEKRNRICASIRDTGFPVVSLDLEGLQRGKMERFLEVKKNACR"
     gene            28568..29674
                     /locus_tag="P5541_03375"
     CDS             28568..29674
                     /locus_tag="P5541_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HRV71049.1"
                     /translation="MLVVDPQGGATPLSLAGALSALPVPEKILENILSEKMTLQGGTS
                     VRLQRIAKPSASGMRVFMDLVVEGDQEEVKRTTAVETLSILEKTSCASGDALGKTASV
                     LDEMIQLSGEDISAGGEIEWSIPYTALASLAFFRFIEETAVPLVSLPVGICTNQAGPG
                     EGIPSSSLSSVISAAAKYSIPIFGLRPGDHGTTVAAMLMLAKTARFTDHLPSMVPEGQ
                     FQGIGPDGTTVRVILSREKKREDVWLVEAALDDATGEEMGRALSTIQEASLEAHILQG
                     IGKKGRPLFILRSLARSEQLDEVLDRFFRDTPTIGVRYWPVGRTRMHREIREGQLVVD
                     GLSLPTRIKISRLDDVIKGKAESDDIAKHLSGKR"
     gene            complement(29730..>29968)
                     /locus_tag="P5541_03380"
     CDS             complement(29730..>29968)
                     /locus_tag="P5541_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV71050.1"
                     /translation="GSGYPRGLKDGEILLQARILAVADVVEAMASHRPYRPTLGLEAA
                     LDEITLNKGKLYDPEAVDACLRLFREKHYRLVGV"
BASE COUNT         6549 a         9219 c         7675 g         6525 t
ORIGIN      
        1 ttcctgtttc ctgtttcctg cttcctgttt cctgtttcct gtttcctgct tcctgtttcc
       61 tgtttcctgt ttccttgtcc ccttgttcct taactcccaa ccggcaccac caccgggaag
      121 ccccgaaata tctcgatcgc caccgggatc ccgtaaactt cgcacaccat ctccggggta
      181 attccctcaa catcggtggc agcataaatc tctccgccct tcatgaagat caccttgtcg
      241 gcgaaccgga gggcagagtt tatgtcgtgc attgtcatga tcgcagccat gccgtggccc
      301 gtgacaatat gcctgatagt cgcaagtatc tcctgctggt tcttcaggtc caggctgctc
      361 gtaggctcgt cgaggagaag gatcctcggt tcctgtacca gggccctggc tatgctgacc
      421 ttctgcagtt cccccccgct catctcatcg agatgcctca gtgaaaggtg ctcaagccca
      481 agcgccctta taatggcatc gaccttgcag atatcttcct tcccgacctt ccatgcaata
      541 tggggtctgc gcccgaggag gatcgcgtca aaggcggtca gcctgccccc ctgcccctgc
      601 tgagccacgt aggcaagatc ccgggcgatc tccatctgct tcatatcaag gagttcccgc
      661 tcctcaacca ttatcgctcc gtccgagggg cgcaggatcc cgttgagaca cttgagaagg
      721 gtcgtctttc cgaccccgtt gggcccgagg atggccagca gttcgttgtt tctcagtccg
      781 aaagagactt cttccagcac gggcctgctg ttgtagcgaa aagaaatgcc ctttacctga
      841 aggatcatct ccggtacccc ctgaccagga ggtaaaggaa catgggggcc ccgacaaagg
      901 aggtcagtat cgccacaggc aggacattgg gagccatcat cacccttgcg gcggtgtctg
      961 ccgcaaggag aagcatggac cccataatgc aggacccggg aataaggaac cggttgtcat
     1021 caccgagcag acgtctcacc atgtggggac agacaagacc aacgaaccct atgaccccca
     1081 caaaggaaat tatcaccgcc gtcaccagcg aggccaggct catgccgaaa aacctgacca
     1141 tccgcacatt caccccgaga ctcgccgcgg tctcgtctcc tgcgtctata gcattgtaat
     1201 tccaggaatt gacgacaaaa taaaaaaaac agagacccgt gacggaagaa atgaccccga
     1261 tctctctcca ccccgccctt gaaagatccc cgaaagtcca gaagaccatg gcagccaact
     1321 ggacatcatc ggcaaagtac tgcaaaaaca tcgttgccgc cgtgcaaaga gagcctatcg
     1381 cgaccccggc cagaatcatg acctcagggg atccccttcg gagacgggcg atcaggataa
     1441 cgaccccggt tgctatcatt gcagcggcaa aggctgaaag ggtggtcata cccggccgca
     1501 ccactgttat ggcgtccgat gccgaactct ggataaatcc ggacccgagg accatcaccg
     1561 aaaaagccgc accaaaggca gcggcctggg atatgccaag ggtaaaagga gaaccaagag
     1621 gattgcccag gaccgactgc atcaccacac ccgccaccga aagtccggca ccggctatga
     1681 cggcagcaat cacctggggc agccttatcc tgaacactac aacgtccagt tgtcttgaga
     1741 catgatccat aaaaagggtc ctgagaacct ctaccggatc ataactgaca gatcctgtcg
     1801 atattgaaaa gactgcaagc gccaggagaa ggacaagccc cagcaggagc agcagaacct
     1861 ttgcctggat atatttcgcg tatcccgccg ggaccgttcc ttcttccaga tgcatcgacc
     1921 ctattccacc ttcgaaagtt ctatttgctt gaagaccgtg ctcccgaatg atctgtccat
     1981 ctcttcataa acaggcttgc cgacaaggaa ggtgtagatc tcgtcggcct ttttcaccgg
     2041 atcgacatcc tcgaacctgt cggggtaaag aaccttgccg atgaaatatg cgtctgcaag
     2101 gatcgaccca tggttctggg agtaccagtt atagggcaga accccgtata tctcaccatt
     2161 ttctaccgca gacaggacct ggaaagcagc ctggtcccgg atctggtaga gaccattgac
     2221 ttcatcccct ccctgaaggg ttgaaagatc gacaaaaatt ttgtccgggt ccatcgaaac
     2281 aaggctctcg acggagaagg tggtctgcgc cagctggtca gacttcgcct tcgaagggtc
     2341 cgccgcgatg tttacggcat taacgaaaag gaacggagga tatgcgggtt ccgtggaagt
     2401 tatgccgtgg ggtcccttgt aggccacacc gccgatgtaa caggtcggcc ttttatcctc
     2461 cggaatgtcg gctgtccttt tccggagatc ctctatggct tcgtcgaaaa aggctatgac
     2521 ctcttttgcc cttttctctt tgccgataac acgccccatc atcaagagag tttcatccat
     2581 cttcttcttt ccgacaccaa gctgcccatg gtcgagaact atcacaggta tgccggtttt
     2641 tttctggagt tccacggggt cgtgacccat attcccgaag gtcttgaaga tgacctgagg
     2701 tgccgggtcg agggccacta tcagttccgg gttgtcgtgc ccgcgaaatt ccccgaaaag
     2761 gggtctgtcc ttcagttcgg gatgggctat gtagtatggc ctggactcga acatgttccg
     2821 cgacttctcc atgtcatcaa cagccactgc aaggtcctgg gcctggaggt agacaaggta
     2881 gcgaagacat cccggcccgg agcatatcac tttcgtgacc ttttccggga tcacgacatc
     2941 cctctcccag gcatctttta ccgtaacctc tgccgatacg caactaaccg aagaaacaat
     3001 aacaacaaac aagaccgcca tgacgagaaa aatccacctg gtccaggaaa ccctttggct
     3061 cattcttatc acccctgttt ttgattcttt agtattacga ttattttatt ttgtaatatt
     3121 aaatggcgca agggccccgg gtcccaatta atggtatcgg gtctcgaact cgtccagaac
     3181 ctcgataaat tgggacggaa gcctctctct tgcccgggat tcgataccct ctggaacccc
     3241 gccaaaacgt gcttccgcca gagcccctgc tatgcaggcc tgggtgtcgg catcccctcc
     3301 gagcgaaacc gccagcctta tcgcctcttc gtaagaggac gcttcgagaa aggcaatgaa
     3361 cgattccggt acgctcccgg gagcagtggc gtcgaaacgg taacccggac ggatctcctc
     3421 aatggtcctg tcgagagata cgccagtgaa tccctcgact ctttttcgca tctcggcgtt
     3481 acccatgccc tttctcgccc agaagacccc cagggctacc gcctgtgccc agataacccc
     3541 caggggatga ccgtgggtag gcaatgagat cttttcagct tccctgatta tcgtctcttc
     3601 gtccccaaag gccctcccta cagggctgac cctcatggcc gccccgttgc caaggctatc
     3661 ggtacttcca ccgggaggcg aaagtaatcg tccaagcgta gcccggctga aacccgcatc
     3721 gggataattt gcggcgtaac gacgcagaca gtcatcgtaa cttcctctcc cgagaaggct
     3781 gtccgctact gcaacggtca gaaccgtatc atcggtaaaa cggctggaag ggagaaaaag
     3841 ttcaaaatct gtggttttca ccggatacct ttcgtaggcc gagcctataa tgtctccagc
     3901 tatggctcct atcatccttc tcaccccttc acatcgtata taatgagatc cggatcacct
     3961 caaaatcaaa ggagcgtaaa cagatgtttc tcaaaaaaac cttcactttt gatgcggccc
     4021 atcgtctgga ccactatcac gggaaatgcg aggcccttca cggccacacc taccgcctcc
     4081 aggtcaccct gcagggaaaa ccggacagcg aggacatggt tatggatttt gtccagctga
     4141 aggacctggt aaaagaacgg gtcatatcaa ggctggatca cgctttcctg aacgacatca
     4201 tcacccagcc cacagccgag aatattgccc tctggatctg gcagcagatc gactgcgaga
     4261 ttcaaaagga agactgctca ctcttttccg tcgaggtctg ggaaacggcc acttcttccg
     4321 ttcttgtcca ccgcgaggac ataatcccgc ggaagggagc gtaatatctt gaaagtcatt
     4381 cctgttcttg acatcatgga cggatccgtc gtccagggct atcgcgggga gcgctccggc
     4441 taccgtccca tatcaagttc catcacgacc tcccctgacc cggtcgaaaa tgccctatcc
     4501 ctgttggcga tcaccggcgg gtcggacata tacgccgccg acctcgacgc aatacagcgc
     4561 aagggagaga acacggaaca gctcaggaat gttgttcgcg agaccgaagc ctcgatatgg
     4621 cttgattcag ggaccggatc actcagaacg gcttcggccg tcctggtctc tcttcccgga
     4681 tcaaaagtgg tgataggttc ggagaccctc gattcggaac ccgatctcag ggatataacc
     4741 acccacattc cctgggaccg gcgcattttc agcctggaca tccagtcagg ggagatactc
     4801 acggggaaat catcccccct caggggactt gggatcccgg aagctctctc ctttctcgaa
     4861 gattcgggat ggagcgaggt aatcatcctc agccttgaca gtgtcggcag cggagatggg
     4921 gctgccctgg acctgatcgc cggaatggtc cagaagttcc ctgacctgtc aatatacgcg
     4981 gccggaggtt gccgctctcc caaggatctt gagaacctgg cggcctgcgg ggcagaaggc
     5041 acacttgtcg ctaccgctct tcaccgccag tggatcaagg cagcggacat tacggagttt
     5101 tgttcatgaa cccgttattc cccgaaaata cttctcttct tgccaccgtc ctgtccgccg
     5161 aggaaacact ggtggcggtc gccggcggag ccggggtcat cgacgtcaag aacccctcgg
     5221 aaggagcttt gggagcaccc gctccatcgg taatagcagc cgtgaggggg gctacccctg
     5281 atcacattca cgtaagcgct gccctcgggg acatgccctt tctgcccgga acagccgctc
     5341 ttgccgccgt cggcgccgcg tcagccggag cctcgattgt caaggtcggt ctttacggac
     5401 cgtcaaatcc cgaagaagcc tttttgctcc ttgaagttac agccggggcc ctgagaaaca
     5461 gtttcccgga aacggtcctg gtcgcaggag cctatgccga cgcttcccgc ttcgggggga
     5521 tcgatccgct ggttgtgccg gagtgcgccg caagagccgg agccgccggg tgtcttctgg
     5581 acacttttga caagtctccg ggacataccc tgccggacat cctcccgctg gacgcgatcg
     5641 aattcttcat caggtcctgc cgcgaaaatg gtcttttttc tgctctcgcg ggatctctgg
     5701 gcacagaaga aactgctctt cttctgcccc tgcggccaga ctaccttgga tacaggggag
     5761 ccctttgcga aaacggcaga accggcagac tgtctggtga aaaggtcagg caaatctcac
     5821 tggccatcac cgaaggatct cgataatacc cggaccgtta acggcatctt cgggaaagcg
     5881 accgttacct atccaggctt ccccgtgaaa aaacccgaac tggtccggaa aggcacgatc
     5941 cagccaccca tggctcgaaa ggcttttggc ggtcatggaa cacccgggaa tgctgtcctt
     6001 cagaaacagg atctttttcg ccccgaggct gacggctacc caacaggcta tgctgtcgga
     6061 agtcacatcc cagtttttcg ggagggggtc ctcctcgcgc attttttccc cgggcaggaa
     6121 taccgggagg agccccttct cccatgccgc agcggcttcc tcgcctgacc tcaccgggat
     6181 acccctgggc atcaggtccg caagcataag cccgttctgg tccattgcaa gcaccgccat
     6241 ccagtgtgac gattcggggc ccaggtaatg gagcgaatca agccttcgga tctcgtcggc
     6301 aataatcccg cccccgggag ctatgatcgc atttacttcc cgcaaagaac tgtccagccg
     6361 gcaacagata tcccgcaggt tgcttctgga taacaggctc ccgccgaact ttacgacaat
     6421 atctgctccg ggccccatcc ctcgagacat catttcgaca ttgaaagaaa cccggcggca
     6481 gcggcgtatg ccggaaggtg agtctcgtaa ccgtccggga tgacccccga aagacctttc
     6541 tcgggcagac cgatcctcct tgcggcctcc gaaaccagga attttccagc tcccgtggga
     6601 ataacgggca aagactcgct gaacctggaa caaacctgaa gcatggcttc tgtaatagcc
     6661 cctatctgct tctctctcag atagaccgcc atcatccttg cctcatgagc acttatgaga
     6721 tcagagtctg cgcaagccag cctggccaga cgggaaacgg cgtgttggac atcggccggt
     6781 cggccgtcgc aagtcggtgc ggaatatgcg gacccatcca tgtcgccaag aacaatgtaa
     6841 cagtccccca tggatgaaaa cctttcatcg gcaaccctgc acatggctcc cctgaccggg
     6901 acgcgatccg caagggtgtc ggggttggat ctgacgaccc ctgtatagac aagttctccc
     6961 tgggtgagtc tctccgtgtc ggtgtttcca agggcggcag gcacccctcc gatgagggga
     7021 ataatgtccg tggtagtgct tcccgtgtcg acccacaatc cggaaccgaa ctggcgggcc
     7081 agccaggcgg cggcggcccg ccagtttgtc gccgcgaaat ctccgggggt acggaaggcc
     7141 tcctcgagag tcttccactc ccctcgaccc gaaatacacc acacctcgct tgatggaaag
     7201 gaccgcccaa aggattccag tacaaaaagg actccttctt tttttgtgct gaaacagtca
     7261 cagagttccg cagtcatggt cacggccatc acggcccttt cggcaccagg gaaacgggat
     7321 cccaggtttt tcaacacttc gtcaagcccg ttcgggccct tccagacctc gaaggaacgg
     7381 cttcccttcc ctgcgggaga accatcagga aaaagggcgg cacacttggt ctggaccccc
     7441 ccgatgtccc agccgataac ggttctgtca gaaaaccagg tcaaggggat catcctttct
     7501 gaaagagact cgcgggacct tcagatcaaa aggaagggag tcggacatac aggcatcaag
     7561 gagtatcttt ccagggttcc acggggcctt tgccccggca acagcaagag gtgtggttag
     7621 cctggggttg acctcaatta caacgggccc ctcgtcggtc ataacaatat ccaccccgac
     7681 aaaacccatc attcccggaa ttgacgacac caccctctgg gcaagatcct caaggacagg
     7741 acatccccac ttcccgcgcc ttgagaaaaa ggcatcctca accccggcaa tgccacccct
     7801 gtagatgaat ttccgccacc caccgggcga tcccgcttcc gatggaataa ccacatcctg
     7861 ggtgttgacc gccaaaagcc ttgctcttcc acttctgctc accaccaggg agaggctcat
     7921 ggcgtccccc cggagaagtg gctggacaag caaaacagcc ctttcgttac ccggggctgc
     7981 aaagaaaaga tcaatttccg tgctttcaca aaaaacggcg cttccttcgc aactggttcc
     8041 ctgcaatggt tttgccgcaa cggggaaacc tgtctctctg gccgcgtcct gaaccgccct
     8101 gacttccctg gcaacacacc accggggaac aggaacgcca ttagtctcca gcgctttcat
     8161 aagaagggcc ttgtttgaag acagggcgac ggcttccgga tccggcccaa gcaaccggac
     8221 cccggtcccg gcagccattc ccgcaagaac cccggaaagc ccatccgttt cgggggctat
     8281 caggacggca aattccctct cgcggcattc actgatgaac cggtccacac cttcaccggg
     8341 gttgaccggt atgacccttc cgtttttctc gaccatcgga aaagagatgt cttttctcaa
     8401 aagaaggctg gcggatatgc catccagctc cgaaaaggcc ctgaaaagcg ccccggccat
     8461 taacagcccc tctccggcaa gtccggcact tgcagaccct gacacagggc ccccgcccga
     8521 aacatactcg aaaatcacaa ggctgcccgg ttccctggac ttcaatgatg acatgattac
     8581 catttcctga gcatttccgg cagggtcccc tcttccaggg ctttcaggac ctcgcagaaa
     8641 agaacggcca cggtcaggtc ggccgtggta ccgggattga aggaattcgc cagatccctc
     8701 aaactggaat caaactccag tacggaacta cgccattttt cgctcccggc cgcgccggat
     8761 ccgaggaccc gagaggcctc gaccgatacc gcggaagcat cttcatttcc ccttttccgg
     8821 gcaataaggg tatcgggaaa acgggaaaga atggtcagga aagtctgggt cacagcatcc
     8881 ctgagagaaa gccccgcttt cgcggaatcg tcaaaggctg gcaggcccgt gttgaaggtg
     8941 atttcgaagt cggtaacata ttctctggca acagaatccc tttctctggc ctcggccata
     9001 gcctccagga aaggtttcga tggcggaacc tctttgacat catatcggtc ggaaccaccc
     9061 agaccaccgg gggacgctat acggatcgcc tggtaaacgt cccgggtgtc tttttccgta
     9121 agcaaacgga gttccatggc cagcctctct ctcagttcac cggtgggtcc tccgatagcg
     9181 gctttggcaa ggggaaaaag aagcagggct atccccaggt tcgtattgca cccggcgact
     9241 tcccttgtat ccattactgc ctgcaggatc aactctccgg tccttttccg gccaagatcg
     9301 gaaaaagacc ggcccatggc ggcgctgctg gccagaaact gctctgccgc aacatccgag
     9361 aaaggcctgt cccaggtcac gttcccgggc ttcagggcag ttatttcgag ctgacacgct
     9421 atctgtgccg cccacagaac atgctcggca cggctcaacc tgtcctcact gcctttcggg
     9481 atgtctctcc cagaatatga tccaccagca cattccccgc gtctatccct gtcgctttct
     9541 ggaaccctct ccacccggga atgccgttga gttccagaag catgttcctg ccgtcgacgg
     9601 ttctcagtat atcgacacca aggtattctg cgtccaccac tttggccgca tcaagcgcca
     9661 gcgattccat cacgatgtca ggctccgccg gggccacctg ggccccctgg gcaacgttcg
     9721 atttccagtt ccttcccgtg cgctgcatgg caccaataac attgccggct accacaaaca
     9781 gcctgtagtc cctgttcgca tgggggagga attcctggag atagaaaata taatgctgct
     9841 gctccagcgc cgaaaaaacc ctgtaggcca tgtccggatc ggagactctg accattccgc
     9901 gcccctccga accgaacagg ggcttcacta caacatcccc gcccagttcc tcgaaggcct
     9961 ccatggcctg atcgaacctc tcgcaggtca ccgttgccgg cacaggcaac cccgccctct
    10021 cgagaagaca gagggtaaaa agtttatcga ctgtcctctc tatgaccgag gcggaattaa
    10081 ttactcttac accaagggat tcgagcctgt ggagcgcgtc aacccggtag atgacctcct
    10141 cgagagaacc accagggagg gacctcacga aaagcatatc cagagattca agatccacac
    10201 ccccggtctc gataaaaggc gaacttccag gtccacacct ggaaacgatc cgggtcgatg
    10261 gcaggcaggg cgcttctatt ccccttctcg cgagagagtc atgaagcgac cggacatgcc
    10321 agccgtcacc tctcccgagg atggctgccc ttaaagtccc cattctttcc tgagcacctg
    10381 agtcgagggg gatcccgccc tgaaaaccct tcccgtggat atgttgttga tagtgaccct
    10441 ggcggggctg aaaagaagcg ggtcgatctt gtagaaatcc ccctcgtact ttctgaagag
    10501 ttcggcaaag ggcgcgccgt aatcactgga ggccgatgat ggcaactttt ctataacagc
    10561 ctcgatgagg gcatcatcgg tctcaaccgc gtaccataca tcgcccccgt agagcaccga
    10621 atcgttggtc caccccatcg cttcgaggga gtttctgccc accggtgcca gcggggccgt
    10681 gccccatccg cttttcacgg tgccgatatc aaaaccgcac tggtgcatct tgtgcatacc
    10741 cgtctcgacc acccttgcca caacctgtgt cgaagccgtt atcgaattgg ttcgtgccac
    10801 gaccatcagc agagatgatg gggagacacc gcattccttc gagagagact ccacgacttc
    10861 ctccggcggc aatttttcgc tctcaagagt cagaaccgcg acatcgcttt tctcgctgta
    10921 gccgatctct cttatgagct cttccctgcc tctgagtatc cgtcccgggc cagacaccat
    10981 ggcaaaataa cttcccgagg agacctgcca cccggcgtac tgggaagcca tacaggccag
    11041 tgaaggcctt ataacagcaa cacacagtcc gggcattgac aacccgtcct catggtagtg
    11101 aacgaaggag acctttccga gtccgctcat gcatatctcg gcgtaaagcc tgcccgcctc
    11161 cagggatccc ggagacctga ctccggagtc tatgaccatg gcgcccccga ctgtctggac
    11221 aaccctgaca tccagttcat cggccttcgc tgccatctgg cgaacaagtt cggcggcttt
    11281 ttcgttaagc ggcttcatgt tcgagtattt cccctttccc gagggacgtg caatcacctg
    11341 tccagcgcac aaaatcccct gccaccgtat ttatgtccac gggaagtccc agtttcgcaa
    11401 ggtaacccat gtaaagcggc acaaaagaag gcctgtatcg acacgccctg acgaacgacg
    11461 gcaggatccc gtctgctccg ccaagggcaa ttatcgtgcc tctcttcatt cccccgcctg
    11521 ctcttctggc caggcggccg aagaccacca tagtcccggc aaccatcctc gaaccagcga
    11581 attcacccgc actgccgccg acggcaatga ggcctctcct catggcatct cctgtcctgc
    11641 tcccggtatt tcccattatc acgactactc cgcccttcat tcccctgctt tcaccaggaa
    11701 gtgccgcggc agtcctgttg ccggcgctgc cggtgacatg caccagccct ccgctcatcc
    11761 cgcacagaca ccagtcgggg atatcgccat ctaccagcag ttggccgccg gacatctctt
    11821 cacccagatg catccccgga aaacctctga ctttcaggat acccttatcc atcttttccc
    11881 ctatcctctt gacatggcca aggttccctg aaaccacgag agagtcgtcc ccggaacgcc
    11941 tgatccggaa aatgtccccc agagaataat ttctgttccc aaccagcacc ggaagagacg
    12001 ctatttcctt ttcggatctc cccaggacgg acccgggcgt aagacacggg gcttccacag
    12061 ggatggtctc cttgataaaa cattccagga ataccgaact attttcgttc atagccatca
    12121 ccagccgacc tttcaaaaaa tttcatgaag cctgaaaagg tggtccccaa gggacccgcc
    12181 atagtttccg gccgttattt gaaggatccc ttcgatacag gccgccctta ttccagttct
    12241 catggcctcc gcgacggaat cgtcgtccag accatcgata actatctcca gcaccgagtt
    12301 ggcatcttcg ggcaaagcgc tttcaacaag tcccctgaga gtcgggcagt aggccacgtt
    12361 cgtggaggcc ggaagaaaag agtaccgtga cccgacctgg cttccgctcc tgaccacccc
    12421 tccggggaag ggaagtatca ctcccgggag atcctccacc gcatccaccg cggcttctgc
    12481 cgcacggagc accgaagggg catccctgcc tatgatcaaa aagttccctc cgcccaccgc
    12541 tttctgaata ccaaaagaat cagataccag gaactctccc tccatgacgg gtattctcca
    12601 gtacctcttt ccttcgagca ttttgcttgc ctggaaggtg tccccgaaat accgcagacc
    12661 cgcgcccacg ggcaccttct cggatccttc gagcgcatcg aagcaagccg tcgtcgggca
    12721 tgtcatgaca gcctgtccta tcctgctgac tagttgtttg cccaggttct tttttgacgg
    12781 agtgaaaaga agaacatgaa caccgggtct tccgtcaggg gtttcggaag gctcaagaca
    12841 tctctctatc cctgcctcgc atccacaccc gatgaccgag gttgcaaaac ccgcaacgct
    12901 ccgtgccgcg gcatcaagcc agcgtctgtt ctccgccgtg acacagaacc tggcccccca
    12961 catgccgaag gcctccgcaa aagtatcgac aatttcgacg ccgtttatcc tcaaggatta
    13021 ttcccccatt catattggtt caacattcca gaccgctcct ttccttgctg cggctgaaac
    13081 agtcacttca agatcttcct gatctttgaa aatccttgcc acctccgccg ccagaccttc
    13141 ggccacttca atgccatcca cgatcccgta gacggcaggc ccccaggaac tctgacccac
    13201 accggcagct cccagttcct tcattgaccg gatgatcctt tccgacaggg gtgaagcaaa
    13261 gcggcccgac tgggccgggg caaaacagtc acccaccatt ctctggatcg aggtaaccgc
    13321 ttccccgaag gcccccaggt cctcttcctc cagcgcggga aggagcctca tcagcgtctc
    13381 cctgcatatg gctcccgaaa cttcggggga tggtcccgga aggtccctga aagcctcgac
    13441 ctccctctcc ccgctcatgc ctgtgcccga accgggaatg gctacaacga aaaaccagtt
    13501 ttcggggatc cttcttcggt aaagaagggg gggcagttca tcctttccct cagttttctt
    13561 ccccccgtct atgaccagac ccccgtcgtc gaagagggcg atcccggcgc ctgacctgcg
    13621 tcccctgccc atcatggacg cgatctccct ggttgttaaa ggcatgtcgt gaagaatgct
    13681 cagtgccttg cctactgtca aagccagttg cgtacccgat cccaggcctg catgaggagg
    13741 aagcccggat cgaagcagga gacgtgagcg ggtctcttcg ggaattcctg tcgtctggta
    13801 gaacttctca gcaaaagaac gggttctatc cgaatcgtct cccccggctt caagaacggg
    13861 ggactccacc gcctcgagat tcatgcaggg gctttccagc cccactccaa tgctgccaaa
    13921 cattctccca agtcctccat ggagatcaag aaaacccaga tgcagcctcg agtgggaact
    13981 aacagtgcag cgtctcaccc aatcccccca gtcagttctc cgacaagacg gagagcaagc
    14041 ctttcttcct caccgcccgt cctttctgtc agttcaaccg ccctccggta ctcggcaaga
    14101 aatggttcct ttcctctgac atgtatcctg gttgcggcca cgagtgcttc aagtatcgcg
    14161 cagcgggatc tgttgaagcc ggtaaagggc ctgcgagact cctcgtacgc gacctcgaag
    14221 agacaatgcc ccctgtctcc ctcaatggat attcttttga gttccagttc cagccacccg
    14281 caaggatcat caataactcc gcacggaacc atgtatgccg gaaaatgacc cggcatatta
    14341 tccctggcaa cagcatggac gaagggaagg atcgaatcgg tgatactcgc gacagcaaaa
    14401 cccttttcgt taaggttcgc ccaggttccc gaatccctga aaggtcgaat cagcacagtt
    14461 gtttcgtcga tcctttccat ccccatggga gcgaaattgt cttctccgtt gccgtttcta
    14521 gtcgaaagaa tgacctcaat aatcatggtc tggagatata cccccaatcc agaggctggt
    14581 cctgcacgta gcgtttaccc agtttcaggg ccgtctcggc cttttccagc tcacggccga
    14641 tataaaaggc gtgggccgca tcgtcgatat ttatctgttc ccatatctct ctggccgtac
    14701 ttcccgtagc aagactgtcg ctgttgaatg ccactattcc ggtggtcgag aggaatattc
    14761 tgtagttttt gtcccgaaca gcggttttca tttcctggag ttcctccggc aaaaaccgct
    14821 ggtgcggaag gtctctcagg gcaaccagcc ccgctttggc atgttttgga aggaccctgg
    14881 aatcgcgggc ccagaacatc aatctcctgg cccggtcaaa ttctgccacc gcgcccgttg
    14941 cccacgaaac taccgaggtc gtcagaacag aattgatcga cagttcggtg catatcgctg
    15001 ccatcactgc agtgaccccc gacgagtccg cctccatcag ttcggtctgg ttgccgatcc
    15061 ccatcagcat cggaatctcg ggataccgcc tgcggaagat gatgtacctc tccagagatt
    15121 cgatcagccc gaaagggaac gggtccagaa ccgggtccgc aaggtacgag attcccgaac
    15181 tttcgaggat ctcgatgttc ctctcaaggg aattcaggct tctgtcccga aagtccggta
    15241 ttacaaccac cctgcatccg agatccagta cagactcgat attgctgctg ttgacgctca
    15301 aaagaagtac ggcaccggct ttggcagctt ctctgaggct tttcccgtcg aaggtgtcca
    15361 cactgaccct gaacccctct tttcggagaa ggcggacctt ccctgccact cctggaaact
    15421 ccccggagac cggaccgcca aggtcgataa catcggcccc gtcttcgcgg taacgggagg
    15481 cactcctgac gagttcgcca tcatggagca gatgagcgtc aactatctcc gccaggatcg
    15541 taatatcatg gccttcgaga gaaacatctt caccttcaag gttaaaaaaa cccggcagat
    15601 ccttcaggtg atcgggcccc ctttttacgg gagtatctcc cgcagcctcc tgcagcatcg
    15661 agatggaccc ctggcaaagc ccgggaatgt agactgcatc gtaacccgct acttctggaa
    15721 gttgccggga catccacccg cattccgcaa gagcggcaac agtgatcggc atgacatgta
    15781 catcgtattc gaagggtggc tccataccct ccaggatgga tctgagggaa ggagccgcaa
    15841 gctgtcccgt caggaagagg tagtgagcca tttttccagc tgctccaatg tcgccgccac
    15901 cctgacaagt gggaaggatg atagcctttc cgcgttttca agatccactt ttcttgaacg
    15961 gacggggaat tctctgcccg aagggtcagc cgagaccacc ccggactcaa ggtcggtggg
    16021 aagaaagaac gacgggacaa gggccttacc cgcctgggca aaaagattcg caggaagagt
    16081 gtcggctatg ccgtttacac actttgccac ggtgttggcc gtcgccggcg cgatcaccag
    16141 tcggtcgaat cgcccaaggg ccatctctcc gcaagaggga gagctggcgg tcctgtcgta
    16201 aacgacccgg accccggatt ttagaataat gtccataaga ccatacatgg ttgtgacctc
    16261 ttcaccggca cgggacaaaa agaacgttac gttcccaagc cgaagagcca actctacaga
    16321 acccttgaga aagtgccctg ctcctgtcag gcaccatgcc gtcccgggag atctaccatc
    16381 cttcaactct gcagccactc ctggagcgtg atcctctcta tggagggttt atcgtttttc
    16441 agttcctccc ggatcgctcc catcgttccg gacctctcgg caaaggcatt atgctggtga
    16501 atgctctcca gattctcctg cctgaccatc acgaaagttt cgtccgggag atcgggatat
    16561 atatctatca ggttcctgaa tacttccctt acaacgtcct cggtgaacat cgggttctga
    16621 tgagccttgt tgacgatgaa gaattcgtcg gatcttttga gcaattcata tgtctctgaa
    16681 ctcatcgaag cttccaggac atggacgaga tgttccgccc tgaccggcca gttcccgccg
    16741 atcagcaggg ttccttttcc cctctggttg tgggaggcca gaggtatcag gccgaggatc
    16801 cgttcggctt cgctgtcgct gaagccatcc tctacgagga gtttttttga atgactcctc
    16861 accatgtcct gggcacaggg acagaccgtc ataccctcaa cttctactcc ggtaacggtc
    16921 ttgactttct cgccgctgca ggaagctatg ccgatgaagg tataaatttc ctccacgctt
    16981 ctccctgaca ggggagtcag tttcttcagg ggatatttcg cccttatgaa gacctcggcc
    17041 ctctcggtcc tctgggtcgc ggctatctgt cgcgcaagtc tctcggccat accctcgata
    17101 gtcgaggatg gttcaaggct gatctcctcg gtgagccttt ccagaacatc gctgaaacgc
    17161 gacatgtgaa ctccggcctg cttctggtcc aggtaggcaa aaagatccat gtccgcgtag
    17221 aaaagggatt cccgaccgtg atcggtcatc ctgatgatcc ttttgaggtt cgtgacacca
    17281 acacggctta gcacaagggg aaaactggga ctgctctgct ggacatctct ttccagtttg
    17341 aaccgaaggc tcttctccac cttcctgatc ccggacagat ccgctttgcc tgaaaggtcc
    17401 cctatgcttt cagagaaccc gggaggaaca atttccggag atatctcggc aaggggaaca
    17461 agggcaaaag ccctttccgc tagcctcgaa tggggtatcg tcagttcgtc cgtcctgaga
    17521 gaaattgaat cgtagagaag aatgtcgata tctatgggcc tgggcaagtt gcgggaagcg
    17581 tccgccctac ccatcctcag ttctatggat ttcacgaacc tcagcatctt atggggctcc
    17641 atgtccgtgc gaagcctaca ggccaggttc agaaacaggg gctgcccatt catcccctcc
    17701 ggctctgtct cgtaaaagga agaaacctct tcgatgaagg cccttccctg gagatactgc
    17761 aaggcctgaa gaatgttgct ctggcgatct cccaggttgg caccgagaag caggtaaacc
    17821 gtatgaagtg acctttcctc tatcggcatg gtacctcctc tttactcggc aggtaacaat
    17881 cctgcaccgg gtagttgtcg aaagagacgg tgtaatactt cccgaaatgt tctcttataa
    17941 ccttctcgac ggaggcgtcg aaggggggag cgacgtgcag ggtcttgccg tggatctctc
    18001 ctgccgcttt cccgtttttg actatgatct caccgccctt gataacccat ctcgggaaat
    18061 ccatctttcg cttgcaaccg ttggcggatc tgcatttggg ctgtctgcag ccatcgtccg
    18121 cgggatggaa tatggctaca tccccatcgg cgccaggtcc caggtgccct ttcttcacaa
    18181 gccccaggac ccgagctgga gcggcacgag taatggtgac gatctccccc agggaatatt
    18241 ccctctttat ctctcccagg atggtacgct tcctgaccga ctcgggcagc gtttcgagca
    18301 cctccatacg gtatgacctg tccatgagca ggtttatgac ctgaggatag cagaagaagg
    18361 gtcccgcgtt gggatgatcc gttgtcagga aaaccctcca gggatctttc gccagcagaa
    18421 aaagctccag gccggtgacc cactgaaggg cgtttacctt gtttttgcgg ctgtaccgta
    18481 tggggactat ccctccgcca gtctcgttct cttcgtcgcc gccaaaccac ttgtggccag
    18541 tcattcttcc aagattgtac tgaaagggac catcggaggt cattgttgtt gccggtccga
    18601 aaactatctg gcccacatca aaggtaacgt tgctgttcgc gtttaccgca tcggccagtt
    18661 cctgcgtcct gttcgcgaat ctcccctttt tgtccttacc ataactcata aactggagat
    18721 gggtcaggtg gagccttccg gaagcttccc tgacagatct cagggtatgt tccgcgctct
    18781 cggtcgtacc cagattcaac ccgtgaagat gcaccccgtg aggaagacca aggctgtcgg
    18841 ccgcccaggt gagggtctcg aggatcttcc ccggtgttat tccaaaaccg taaacatcct
    18901 gatcccagct gccaacattg gatctggaag atttccagtt ctctaccccg ccagggttca
    18961 cgacctttat tccgtatccc ctggccgcct tgagggtcca ggcgatgaag tccctggcga
    19021 gttccatctc tcccttggca atacagtcga agaggaactc gttgttcccc atcgttataa
    19081 gcatccccct gtcaaggaaa ggcatgtcct caagttcctc gtgggagtgt ctcgctacaa
    19141 ggggagcgca agccgcctcc atcaccgtcg tataacccat ctcggcatac atgcaacccg
    19201 tcatgtaggg cgttggaaca gtgaaccccg cccctgaacg tccaccctcc aggcggctcc
    19261 tcttgtgggc agcatgctcc tccgggcaca tctttcgacc gtgattgacc tttgcaccag
    19321 caatatggga gtgaagatca acccctccgg gcataacgtt cattcccgcg gcatcgatga
    19381 tcatcgcccc gttcctgtcc gcctcgtccg gtgcgaccac cgaaccgtcc ctgatccaga
    19441 catcacggac ttccccttct ataccattgg cgggatcgta aagccgtccg ccgattattc
    19501 ctacagtttt ggcgctcatc tgctcacttc ctccagaaga cggtcgaata tttcttcatc
    19561 ggtcgggcga tgcctctcac ggaaagccct tgtcctgaca ggaacgccgt ccatcctgta
    19621 ataggtgcct tcggcgtcga cgccgtactg ggccccgggc aaatatacgg tagcccccct
    19681 ggccgtaaga gggaacgccg gatcgacgac aatgtgcggt atggcatcga gccaggccac
    19741 cgcctcggca gggaaatggg ccttggggtc ggaggcaacg atcagcgcgg catcagcctc
    19801 atgccttgcc agcatatcca cggccgtgaa ctctccaggg ccgtaaaccg ggacccctcc
    19861 cgccaggctg acagcgaatg gatatcccgt ctgccaggtc agaacggcat cggcaccggc
    19921 aacattcccg tgacctctca tggctatcac agaaaaacgg gtatgggcgt tcagatcccg
    19981 aacaaggttg aagtactgcc ccacgttgag gtccttcccg ctggtcatgg tgagccccat
    20041 cccgaagaag gcaacaccgt agcggcaacc cttcatctct tctgcaaggg ataccagttc
    20101 ctcccgggaa ataccaccaa cctccgcggc ttccatactt ttgccctgga caagagcgcg
    20161 gagggcagaa agaacttcat aatcacagcc ctgctttacc ctgaggaaac ggtcaacggc
    20221 ctttgcgctg ggagtctccc tgacgtccac aagaacggcg cggcgtccct ttcttccttc
    20281 cggtacaaag cggccctttg gcatcagtga atacctggtg aaatggcggc tgtgagcgat
    20341 catggggttg catccccaga agagaaggaa gtcagccctg ttcatggcct ctcccaggct
    20401 acacgtcggt tccccgaagg tctgcatggc tatcccggtc ggaccgtgac atacggagga
    20461 ggtcgtatcg atgacggcac ctatgcggtc cccgagggcc acgcacttcc tctgtgcctc
    20521 agacgcggtt gaactcagcc cgtagaaaag cgggcttctg gcatttttca ggatagaggc
    20581 ggcctctcct atggccttgt cccaggatac cggttctccc ctgaaggcag gagagacagg
    20641 agaaggatcg tattcctcca tgtgcccctt tccgttagcg caggctcctt ttatctcgat
    20701 ggtcccgtca tcctttgaag agacctttat gtcgtcgcag agacatccac agaaggtaca
    20761 tacgacgctg tccatgttct tcattccgga gtcacctcca cgcggatccc cttgaaggag
    20821 ggcatgccgc acccgccggt atcgggcccg acaagcctgt tggcgacagg tcccatcgga
    20881 acgaaaacca tatctgcggg cattcccggg tcagactccg accggaccga tatctctccg
    20941 ctatcgctcc tgagtctcag ggtgcacccc acctcaaccc ccatggtttt gaacatttcc
    21001 gggtgcatca ccgccaccga tacggcgttg gcataaaaat ccccggtctt gccgaggtgc
    21061 atgcctttgg tctgtgcctg tgtccgcccg gttatgagaa cgcagttccg ggagttctct
    21121 ttttcagcca ttttcccgat ttcctccttc acagagcgct acaagttccg aatagacctt
    21181 cccggcctct tccgcaaggc gcccgataca ctcctcccct tccggggcgg aggaaaaaac
    21241 cgtacacacc gggtcccccg gtgagaatac atcccccgtc catggaacat cccttcgccc
    21301 cgcttcactc caggcaaggg tgtcgggagc cgcgcaatcc ctgacggcgt aaactatgcc
    21361 ctttgcgcga aaaggacctt ctccgcagaa cacgggcctt tttccccgaa gcctttctcc
    21421 ccggcaggcg gccagatgga gcgaaaacaa ctcccccttg tcacctgcgg gtctgaatag
    21481 ttccatggat cccgaatatc tggggttaac ctctatgact gccaccgaac cgtccggccc
    21541 caggatcata tctatcccgc tgaaacctct caggccaagc ctgtccgaaa tgatctccgc
    21601 gtaatatgac gctttttcat aaaaacgggt cgcatcttcc tgccttgcgg gaaggggcgt
    21661 gatgttgccg caccacctgt aaccctttcc ccctagaaaa gatgttccgg tcaactgttc
    21721 ggaaaaaccc gtgatcagtg ctccatccct gtgcgctatg aaggagacgg agactggcct
    21781 cccgggaata cgtctctgga gtatctcccc gctttcgatc cttcccccgc ggtactcgga
    21841 aacacccgac cccccgccgg acaggactct ttttcttaac cagcgtgcct cattggcgga
    21901 cctgtcaggc tcgtcggccg ccctcccttc ctctcccggg ccaagcacct ccggaaaagg
    21961 tattgactcc tcgcgacaca gggaacccag gatcgaggga tccctcgctt tcccgaggac
    22021 atgaacgctg ttgcctgcta tcagcctccc gcgggcaatg gcctctacaa gtcccggacg
    22081 attctccatt cccccgccgt agacaaagat cccgcccctg gcccggcctt cacaggcctt
    22141 gaggatcgcc tcatccgtga aaggcagtcc aaaatggcgg cgggtggata tattctcgac
    22201 agaagacgga tgatccctgt cgccaaaggc atcaacggct gttatgttcc ggaaacccga
    22261 accgcatgcc agatcggcca gcccgcgggt gctgaaagca actatcagaa gattcctgtc
    22321 aatctttgtt gaactccttg acggcctggt aaacctcggc cgcatccaga accagttcgt
    22381 tgctctcgaa aagcctggct acgcaactgc gctggacctt cattttgagc ccacctatcg
    22441 cgatgggtcc gaaaaccagt tggccatcga tatccttgcc atcccagtgg ggcttgatat
    22501 tctctatccc cgatggggga acagcgttga ggtctccaag ggccttcagg gtcgcgtgac
    22561 ccttccagac cgattccggc agaagcatct tcccggccgc accggtcgaa agaactacga
    22621 ccgctccctc cagagccttt ctgaccgatg cctcgtcccc cgattcggct ggttcagccg
    22681 aaacaccgaa cctctccttt aactgggcac aggcagcctt tgccttgtcc attttccggg
    22741 atgtcagcgt gaccatcgcg ccctcaccgg caaagaacag agccgcgcga agtcccaccg
    22801 gaccagttcc ggcaagtacc accgcctttt ttccctttag gtcaaccgct cccgcaagct
    22861 ttgcgacggc agcggcggca gttgtgttgc agccgttgga atcgagcata acggaatttc
    22921 tcagtggccc gaaaaaagcg cccttgaccg catccaggac agcttctccc gccgcgacat
    22981 cggagccgcc cacgaatacg gcactgtttt tcatagaatc tccacccctg gtgaacatta
    23041 ctccgtggac aaggggtacc gctgtttcgg gagttatcga tccatgctgc aatatgatgt
    23101 cggctcctcc gtcataggca gttaccgcat caaaaacact tggctgcgga tcagtgtcga
    23161 gttggagcaa tatttttttc aatctgtttc cactcccttc cgagaatccc ggccccgctc
    23221 aacgggaaga tccatcccgc agggaaaaga ccaggccaca gacatttaca ggttcaccac
    23281 tcatgcaggc aagttcaacg gcggccttga agcttttgaa ggagatcgcg ctgcccggat
    23341 tgaattttct gagcgcagcc tcaagatcgt cgtattccgc cttgcaggag accacatctt
    23401 ccatggtccc cgcgaggatc ttgatacgca cctcggcgta atgatcatcc cgatcccggg
    23461 agcctacccg gactacggaa tgctttacgg gggtaaacgg gaaatgctcc ggttcaaaga
    23521 ctctcatccc aagagtatct agttcaagag caaggaaggc gccaagttcc tccaggcatg
    23581 atctcggggc atcgatcaaa aaaacgaact cgcttctgcc cttcttggtg atcgccggaa
    23641 tggcgtggac gctcgaagcc cctctctcca taagaccttc aatcaggtat ggaagccctt
    23701 ctccagtcac gttgtccgcg ttgacgacaa gaagcatcac cgtcacctcc ctcaacccgt
    23761 attctaatcg gaaaggggcc ccgcagggcc ccctttgtca tatcataccc aggctaaagc
    23821 tctgttacgt cctccatatc cttcatgagg cggatcgcaa gatttgccac actgcgggcg
    23881 ctttccttgg ctcccgggat cgcgttggtg atgcaggcac ccttttcagc gcactttccg
    23941 gcatccttgg cactcatctt tccggatacc gcagcgactg ccgccgggat aacaacgttg
    24001 acggtgcagc agcccacgta catcagttcc tgggcggcta cggaggctac agtcacgggg
    24061 atggtccatt cggacttctt gttgaggacc acatcaggag cgaccttgtg agaaagcccc
    24121 tcgatcttga actccttgcc gtcgactgtc acaaccgcat cgatatcagc atcgaaaccc
    24181 cagtaggtct cggcgaaggg gtggttcctg cgggctccgc ccttgagttt ggtgaactcg
    24241 agggtgatct tctttccgaa catgcctccc agcatcatgc cggtgcgctg ctcgaccttc
    24301 tgcttgcgct ggttgtcgat ctccatgcag atgtcctcaa gagacttgcc tgcctgaagc
    24361 ccgtcccagg tgaacttggc aagacggaac tgcagattgt tcctgatccc ctgggtaccc
    24421 ttgataacag cagccgtaac cgggccacgc attacctgct cggccttgtg ggcaaccatg
    24481 ttgcccgaag cggccgaaat ttcgggatcc agccattcgt tgagtttcat atcgtggatc
    24541 atgtcggctg ccgcctcgac atcgccgtcg ctctcgatca gcttcatgga ggcaactacg
    24601 cagtcgctca cgaggtgagc aaggggcggg ttgacggggc cggcgccaaa gcctgctccg
    24661 atcatcgggc cttcctggaa gcacgccatc atctcgttga aggtcatcat tccgaccact
    24721 gtcggtgcgc cgacgtcttt gaggatcatt cccagatcac cgataagagc ggcggtgtca
    24781 aactccttgc ctgtgccgcg catgtgcagt ttctcgggaa gtcccgcagc ctggacggct
    24841 ccctttccta ccaggtatcc cgtcccctgg acgaaaaatt cgccgtagtc ctggccaatc
    24901 atgccatcgg gattgatgat ctcgaggata gcggcagcag cgagtgccgc ggaaagccac
    24961 gggttggggt tgatgcccac gccctggtaa gccttgaaca tggcctccac gccgaccttg
    25021 gttcccttca tggccagatc gtggtagatc atatcctctc caagcacgct gtggccgtaa
    25081 atggcgcctc ccgccacgaa gggggggacg acggctccgc tgaaacggca gatctcgcct
    25141 ttttctatag catcataaat agccttgacc gccggaaaac cggagatctt gttcgtgagt
    25201 ttcgatgtcg ggatggcctg gactcctgct ctgcttgctc cggccttcat ccttgcgagg
    25261 gatccaagtt tcctgttgcc cgcggggact ccggccctgg aggccgttcc ggcaaggttc
    25321 agaaaaacag caacgatgag agccgcgttt gcagaatcgg ctcctgcgtc cttggccgct
    25381 ttgatgcctg cttccacgat catgtcgaca ggaagattgt ccatattggc atcggacagt
    25441 ttcgtgcttc ttccctgaag tatctccttg accattacgt tggccgcgct gtaaacggcc
    25501 aggttgagca tcccgtgtcc cttggtgatg gcttcgagac ggtcactggt ttcattttcc
    25561 acgaatgtgt tggccgccgc gatcgcagaa taaagttcct tatgcattat ttttgcacct
    25621 cccaagattt ggatctttgc ctggaatagt atccagtctg ttgcggatgt ctcctttgcg
    25681 gtctgccgtt atgcaccccc cttcaccttg gtaacagccc aacagtcccc gtgtcaaaaa
    25741 cccggagcca gaggcggttg ttgaatctcc caggccagat aatgagggct gtgtagggcg
    25801 agagatcttg ggcaagagtt ccgcaagggg aaaacccgga ggggccgggc tctccccaga
    25861 gtgccgctac cttttaagag taccgcaggt ttcttttatt tcgggttcat gtgtgtgtgc
    25921 gtgttcatgt gtgtgtgcgt gtgcgtgttc atgtgcgtgt tcatggtcat ggtgatcgtg
    25981 gtcatggtga tcatgagcgg caccaaaatg ttttttctca agaaggacac cctgaagaac
    26041 gtttaccagg tcggactcat gatctccgat gccataaccc gtcttttcga ccactccgga
    26101 agggagggtc ccgaaactgt cgacaaaaac cttcagcagg accgcccccg tgggagtcgt
    26161 acgttccatg gcctgaccgc ttgaatagac agggatacct tccagaagtt tcatcgttgc
    26221 gggggccgga accggaagtt ccccgtgagc gcattttact gtcccggatc ccacgttcac
    26281 cggtgaggag acaacctcgg ctggcgccag cacatccatg aggacacagg aaccgacgat
    26341 gtccacgatg gagtcaactg ctccaacctc gtgaaagtga acgctttcgg ggggcatgcc
    26401 gtggacaaaa ccctccgcct cggcaagcag cctgaaggcg gtctcggcat tctttgcggc
    26461 agcaggactt atatcgcttt ttgatatgat ctccagtact tccttcaatc ccctgtgaga
    26521 atgcttctcg tcgcacctca cctttacatc cactcctgtg atcccgccct ttttgctcct
    26581 cgaaacatcc accctgtagc tttccagggc gaggcctttc aggaatggct gaatgtcatc
    26641 cttcccaaga agatccagaa ggagcccgag catcatgtct ccgctgatgc ccgaaaaaca
    26701 gttgaggaaa agtactttct tcatcgagat cctccttccg gatcaccgga attttttttc
    26761 cttatcataa gagcggcagc gtagcctgct cctattccat tgtcgatgtt caccacggaa
    26821 actcccaacg cgcaggaatt gagcatcgtc agaagcggtc ctattccgtt cagggcggcc
    26881 ccgtaaccca cacttgtggg gaccgcgaca acaggacagc tcgctatccc ggccactaca
    26941 cccggcaatg caccgtccat acccgcgacg gcaacaatgg cgtcagcatc gagtagcatg
    27001 tcaaggtgag ccagaaggcg gtggagcccg gctactccaa catcaaaaag gcgtctgacg
    27061 ttgcagccca ttatctctgc cgtcacagcc gcctcctctg cgacaggaac atcgcaactg
    27121 cccccggtga caacaagcat gagtccccgc gcactttcac cgcggtcagg ccagaggcgc
    27181 cccattcgag gcccctgaag atattcaaga tcgggaagga tcggcagaag gatttcgtgc
    27241 tgttcggcgg acatacgtga aaagagagcc ttgcccggat gttccgaaag ggcaccggcg
    27301 atccttttca attgatcagg ggatttgccg ggacaataga ttatctccgg aaaaccgttt
    27361 ctcaaagcac ggtgggtgtc aagcttgacc tcgccgaggt cacggaaggg cagtcccttg
    27421 agggatctga caaagctgtc gacggagatt tcctctctcc tgagcttgtc ggccaacagg
    27481 cggagttctc tttcgtccat aagaaatcaa ggctccattc cagccgggag gtgggaggtg
    27541 aaaatgttga catcttccgc ctctttaccg agaaggaata actcgtgccc cggaaccttt
    27601 aatttgatat cacgtatcat tatacacata aactcgaatg tttcaagatg gtgcacatag
    27661 tgaaaaaaag ttcttttggg ctgtcctgcc gaaacccgga ctatgccgtg caaatatggt
    27721 atcgttttcc ccgtagtaaa tatgacagaa ggagtcatct ttttcatgga caaacccgga
    27781 gtggtgatcc gctttttcca ggaatacccg gggacgcttg tcctcctttc ggggggggta
    27841 gacagcgcgg ttctggctct tctcgcctgc cgtggaacag gagggactac cagggctcta
    27901 tccttcagaa ccccactggt caagaagagc gaaacggcca tggctctcga ggtcgcctct
    27961 tttctcggaa tagaatgtca cgttcatgat gttgacgtga tctcggaccc cagggtcagg
    28021 gccaactgca gggatcgctg ttttggctgc cgcaagatac ttcacgcgga ggccaaaaaa
    28081 ttcgccctct caggcggctt ttcaggtatt gccgatggag tccaggccga cgagattcac
    28141 gaacctcgcc ccggcctgat agccgcagaa gaagacggga tcattcaccc ccttgccgat
    28201 gcaggcctga ccaaaaagga gataagagaa atcgcccgcg aggctggact gcccaacagc
    28261 gaaagacccg cggccccatg cctggctacc aggttccccc cggacattcc cctttcggct
    28321 ggctgggccg aaaagatcga ggcgggcgag gaaatactgg ccgccgaagg tttctccaac
    28381 tcccgaattc gctggttccc tcccggggcc gcatcgatag aaatcgacga gaaagacatt
    28441 ccctcctttt gggagaagcg caaccggata tgcgcttcaa tcagggacac gggctttcct
    28501 gtcgtctccc tggacctcga ggggctgcaa aggggaaaga tggagaggtt cctggaggtg
    28561 aagaagaatg cttgtcgttg acccacaggg tggagcaaca cctctgtctc tggctggggc
    28621 tctttccgcc ctgccggtgc cggaaaagat ccttgaaaat atcctttcag aaaagatgac
    28681 cctgcagggc gggacctcgg tcagactgca gcgaatagcc aaaccttcag ccagcggcat
    28741 gagggttttc atggacttag tggttgaagg cgatcaggag gaagtgaaaa gaactactgc
    28801 cgtggaaacc ctctcgattc ttgaaaaaac ttcttgcgcc tctggggacg ccctcgggaa
    28861 aaccgcctcg gtccttgacg aaatgatcca actctccggt gaagacatct ccgctggggg
    28921 agaaatcgaa tggtccattc cgtacacggc cctggcctct ctggccttct tccggtttat
    28981 cgaggagacc gctgtccctc ttgtgtctct tcccgtcggg atatgcacaa atcaggccgg
    29041 acccggagaa gggatcccct cttcatccct ttccagtgtg atctccgcag cggcaaaata
    29101 cagcatccca attttcggat taaggccagg cgaccatggg accacggtcg ccgcaatgct
    29161 gatgcttgca aaaaccgctc gatttaccga tcacctccca tcgatggtcc cggagggaca
    29221 gttccagggt ataggccctg acgggaccac tgtcagagtg atcctgtcga gggagaaaaa
    29281 gcgcgaggat gtctggctgg tggaggccgc ccttgacgac gcgaccggcg aggagatggg
    29341 cagggccctg tccacaattc aggaggcatc tctcgaagca catattctgc agggaatagg
    29401 caaaaaggga aggcccctgt tcatcctgag gtctctggcc agatccgaac aactggacga
    29461 agtcctggac cgctttttca gggacacccc cacgataggg gtccgctact ggcccgtggg
    29521 acgtacaagg atgcaccgcg aaatcaggga ggggcaactc gtcgtcgacg gcctgagcct
    29581 gcccacaagg ataaagatct cgcgcctcga cgatgtcatc aagggaaagg cagaatccga
    29641 cgatatcgca aagcaccttt cgggaaaacg ctgatccccc gtgactaccc ccgcttgaag
    29701 gatttgcggt cagaggaagg ggcctggaac tagaccccga ccaggcggta gtgtttttcc
    29761 ctgaaaagtc tgaggcaggc gtcgactgct tcggggtcat agagcttccc cttgttgagt
    29821 gttatttcat ccagtgccgc ctcgagcccc agggtgggac ggtagggtct gtgggaagcc
    29881 atggcctcaa caacgtccgc aacggccagt atgcgggcct ggagaagtat ctcaccatct
    29941 ttaaggcccc tggggtaacc cgaaccat
//
LOCUS       DAOTIQ010000014        28763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1858_length_28763_cov_7.930298, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000014 DAOTIQ010000000
VERSION     DAOTIQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 28763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28763
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1858_length_28763_cov_7.930298"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..807)
                     /gene="prxU"
                     /locus_tag="P5541_03385"
     CDS             complement(136..807)
                     /gene="prxU"
                     /locus_tag="P5541_03385"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084511687.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(598..600),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HRV71051.1"
                     /translation="MVGEAEPAVEEKSETAKKEVPRMSVMVGRKAPDFEAPVYHQGKF
                     GSVKLSDSLGKWVVLCFYPGDFTFVUATEVSAVADKYKEFQELGVDVLSVSVDSQFVH
                     KIWNDNEISKMVEGGVPFPMISDAGGNLGKIYGVYDENAGVDVRGRFIIDPDGVIQGM
                     EVLTPPVGRNVEETIRQIKAFQHVRASKGKEATPSGWQPGKDTLKPGPELVGNVWKIW
                     KPGKN"
     gene            963..1157
                     /locus_tag="P5541_03390"
     CDS             963..1157
                     /locus_tag="P5541_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71052.1"
                     /translation="MGRIQGSRKLVAGDWESRGKGVNGQRIGRPGRQETGDWWQGRSL
                     VLTKKRKVFSVWLHPLCPLW"
     gene            1228..2529
                     /gene="kynU"
                     /locus_tag="P5541_03395"
     CDS             1228..2529
                     /gene="kynU"
                     /locus_tag="P5541_03395"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRV71053.1"
                     /translation="MGVLSGELASEEKFATEMDSRDILAGFRERFHLLRDVIYMDGNS
                     LGLCPKDSGEAVKRALDEWECLGIRGWLEGNPPWFEISERTGAMAASIVGAGPDEVVM
                     TGTTTVNLHSLVSTFYRPSKELHVILADEINFPSDLYALESQTAIKGFTPEKSLRLVK
                     SSDGRTLDEREIISAMGPDVCLVLLPSVLYRSGQLLDMELLTREAHSRGILVGFDCSH
                     SAGAVPHHFDDWEVDFAFWCSYKYMNGGPGSPAFLYVNRRHFGAMPALAGWFGYEKSR
                     QFEMNRDFIPAGNAGAWQISSPSIFGSAATAAALKVTLEAGIERIRAKSIGLTSFFVR
                     LVDSYLSGEPYRFSVGTPRDPERRSGHIALEHPDEAMRINEALIARGVIPDFRPPNVI
                     RIAPVALYTTYHDVWQVAVHLKEIMDGREYERFSKERKAVS"
     gene            complement(3107..4576)
                     /locus_tag="P5541_03400"
     CDS             complement(3107..4576)
                     /locus_tag="P5541_03400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRV71054.1"
                     /translation="MKIETYGLSPAEWNGDVLVLFLFEDEMDPLEKTAEDIRPTLAEA
                     VSRVDFRAKADSFLAVNVPGGQVKSVYLVGLGSREKCAPDNWRRGAASAVRRAGKDRF
                     TKMMVLPCDGAKEEAAKAIVEGALLGEYRFDRYKSVEETSEKPLKLEELYIHGGDAAS
                     VEKGLVTAAAQNFARDLANEPGNVISPATLAEKARKMSEESGLECVVLDEKKMKDLGL
                     NGILAVGGGSHNAPRLVHMVYRPSGNATGKVAFVGKGLTFDSGGLNIKPGDFMRSMKG
                     DKTGACNVIAIMKAVSELKPSLEVHGFIGAAENMPGGGAYRPDDIIRMFNGKTVEVDN
                     TDAEGRVVLGDVLAYASKQEPSVIVDMATLTGACVVALGGYTAGIFTNDVELASSFME
                     VSRSTGERLWELPMDDERLKKKIRSEIADIVNSGGRYGGAITAAMFLREFVDEGIKWL
                     HMDIAGVDNSDEEYGSYCKGATGFGVRTCLEWLSKYESR"
     gene            4637..9112
                     /locus_tag="P5541_03405"
     CDS             4637..9112
                     /locus_tag="P5541_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRV71055.1"
                     /translation="MTAIPGDLFVGFDIGSAALHCSVLGNDSSVLFSPSPILHLGKPF
                     DSLHHLWLEILDRFREDRIASTSFTGMGSTCFPEVFPGVMREHDSLCIVRGASITSPG
                     TEIVIHLGARDACFFRVAGEGKRPILIDWSTSSKCGAGSGTLLEKQVRRLFPFGGDNT
                     EDFGEALAKAEESARGFDGAGAYNARCGVVLQSDLIHDQNDGVERGLIMARLFRTIAS
                     SIVQDIIGNRDLEAGRRACVTGWLAGCSPILRHIGEITSLDVFRAPDFANVGAIGAAA
                     IALERGNNSVADMGMLEEVSSFARSKRQYMPSLSNSVSRVFIHKSPRTASRDNSPASS
                     GAVLGVDGGSTTTKAVVISLDTGEMLGGEYVPTHGNPLGALAIVMDRLSSSLCSRTIE
                     AVCTTGSARVLFEKVLSSPSLRKRLEGEGFKVHDGAVDEITCHAEGIRSMDSGVDTIF
                     EIGGQDMKFTTFRRDGLKATDEVDEARMNYSCQAGAGQTLENMAAIIGLDVKSSLQEE
                     ALKADRVPAIDATCGVFMEMEEHRLIAENHTVPEIAAAIVRATASSYFNRFVGGGRHV
                     KEKCSCQGGPALGKAFLAAMADVTGREIHAYPNRELMGAMGAAILARRAVLNSRMEGI
                     PSASAFRGWDAAQKPFASSEKLCRDHFGKDSCGKRNCLLKIFSIGGEEIVSGGFCPLG
                     NSEGAGKTRPDHVAVFHSLLEKHFEGIRFDELYAAEDDQRPRIGIRRCSTTIGGMGVW
                     ASALFSSLGFLPILSPVSDERISRLGLSHSSTDFCVAMKISAGHTALMAGDKRVDHVF
                     IPSFVDRARDEEPPHTFCIYTEAEGFLPGDDLGLSASRVIRPVWHLGDREQMARTLCE
                     ELSLAGYDMPLSKIMSAFQNADEAKRSFDAEIARVGDRFLESIESKDEPGYVGLGRDY
                     VVLDPAASSDTGRMFSTLRGMNYIPQVFLTHLFDSVPVDDLVSNEYWEQSSSILKASI
                     FTARHERLYPIRQMNFACGPDSIKFLMEDEIFRRAEKPFLHLLTDAQTNNAPFVTRAE
                     AFERVVAKRHPSQKPFSAFSFIREKGNENTGRRWVIPSMGNASLLGAAVGRSWGLDTV
                     VAPTATADAREAAERLISTETCFPLKGVIGDVISYLEKEARKNGIEAVNSRYLVFLPT
                     TSGPCRFGKYAEVMGLLLDSLGFTGVPVLSPSSHNGYLDSKTLGLPWSPVARTGFLRD
                     IFNSIIASDLYDDLVLRFRPYATDSEIFRKKASERLAVLERTIESSGASLRAISGWGE
                     KTAQEFAALSSPGIPRFPLVLYLGEIYMRQHDLYTRNAIEMLERTGLELVRGPVSEWL
                     RYVVHISRRKNPRLEYIAASMYMNFAQRSSDKVARGPLADRKVLPHPEETIPWIEKAG
                     IYHGDILGESGISVGIFNAFLKGDMAFHSGNPVCGIFHVGPFTCMQEGVATARIRGML
                     KAARRKEPGMIVPVMNASFGESPNPNLEAEVAAFREQCLLLRDSLMRPSRTEKRA"
     gene            9234..9578
                     /locus_tag="P5541_03410"
     CDS             9234..9578
                     /locus_tag="P5541_03410"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV71056.1"
                     /translation="MWKIVFLDKAVEEEFQALTLKAKAKFDRIEDLIRLYGLENIGRP
                     YVAPVADKIWEIRLKTSDSNARGLYATKRGKVIVILRFFEKKSQKIPKNEFSLAKERL
                     KKISDQDSRLGG"
     gene            9585..9860
                     /locus_tag="P5541_03415"
     CDS             9585..9860
                     /locus_tag="P5541_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV71057.1"
                     /translation="MPTVPYSKIKKELHEDPDFLREYNSLADEFQIAEALIRARTAAG
                     LTQKEVAERMGTTQSAVARMESGRSVSLKSLKKYARSTHSRITLKIE"
     gene            9883..10428
                     /locus_tag="P5541_03420"
     CDS             9883..10428
                     /locus_tag="P5541_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV71058.1"
                     /translation="MSFLELAGKRRSVRNFLPDPVPRETIERCLEAARLAPSACNSQP
                     WSFLVADKPETSARIAEAAFSGLYSMNTFAKKAPVHIVVIRENPKPAAMFGGFMRGMD
                     FTLVDIGIACEHIVLQAAEENIGSCFFGWFDEKKVKKLLALPRRSRVDLIISLGFTDR
                     PLAAPKQRKTLEEIRRYVSVV"
     gene            10469..10930
                     /locus_tag="P5541_03425"
     CDS             10469..10930
                     /locus_tag="P5541_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV71059.1"
                     /translation="MPRKILVCVEMGDLAAKLVDYGNSLAHRLDVEVSFLHVLPNPHM
                     WKGYGTWIQPDVTKDAKESARKKIQYLIKKAEEKHPERGEHEHQILFGQGDTSEIIIN
                     TAKEGGFNLIVIGFKGSSGIEHMMVGSTTTNVTRYSHCSVLVYRPGEENPI"
     gene            10978..12924
                     /locus_tag="P5541_03430"
     CDS             10978..12924
                     /locus_tag="P5541_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRV71060.1"
                     /translation="MIRCSGIFLVLGGKQVFEGLSLSVPKGTRLGIVGDNGAGKTTLF
                     RLINGEVSPDRGDVSIPSSSRLGYLPQDLVEIGSDRLMDFLKDRAGISSLEREIARIG
                     EDLSVPGLEEQKRRGLIERHERAVRRFESAEGYGFEARASKILKGLGFRDGDGARNCT
                     EFSGGWKMRASLASILLCRPDILLLDEPTNHLDTESMEWLESYLLSFPGTLLAISHDR
                     RFLDRLTTSTAELSGGKTRLYSGNFSSYVEKREKELEIEEKSRKRQAKETARTMEFIE
                     RFRYKATKATQVQSRIRKLDKTVTLEAQSAARRVSMRFPECPRSGLEVIRAEDVAHSY
                     EGKTVFSGVDLTIRRGERTALVGINGAGKSTLTRLLAGKENPSEGTVRMGHRVISAFF
                     SQESADNLDYGHSVWDEIRKAPSVFDEPQRRNLLGSFLFSGNDIEKPVGVLSGGEKSR
                     LALLKILLSKTNLLILDEPTNHLDMATRDLFQEALLGYGGTIVIVSHDRDFLDNLVDR
                     VIEIRDGKIFDYPGNYSWFIEKREAARIAAGRDETSKTGDLPQDGGQKEKKRLEAETR
                     NRIYRERKKFLDKLSPIEEGIERAEKEIASIDTSLADPLVLSNSEKVKRLMVERSRLE
                     KESGAMMQKWEEIMSSMEAACPEE"
     gene            complement(12941..13210)
                     /locus_tag="P5541_03435"
     CDS             complement(12941..13210)
                     /locus_tag="P5541_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71061.1"
                     /translation="MLTRGIKGTPLYIIVAGFAVLLTGLAVTPLRWIGLALLFLGVFM
                     HLSNCCRKKACPFCGARIPCDSEKCSFCGKWVAPCGKGDPEVKKQ"
     gene            complement(13273..14172)
                     /locus_tag="P5541_03440"
     CDS             complement(13273..14172)
                     /locus_tag="P5541_03440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV71062.1"
                     /translation="MKVLLRSKTGIAIATAMILFMSLVFVAEALSDRPLNLVAPGETR
                     IGSPFLVYVSAPKIIEDLIITWEGATVSPEVEILAGGCEAMVLVGTDVGSSKPGPGVL
                     EVSSVIEGESFSITRHIDVIHREFPVQRLSLPAGMVTPPEGVLERIKIEREESLKALE
                     TMTSARKWTIPFTRPVPGVITSPYGFRRILNGQPRAPHRGVDYRAREGDPILAAADGT
                     VVLSADHYYAGECLYVDHGNGVVTVYMHLSRRLAEEGDLVSAGDVIALAGRTGRVTGP
                     HLHFGLSLQGKMVDPEPLLEKSK"
     gene            complement(14198..15268)
                     /gene="rarD"
                     /locus_tag="P5541_03445"
     CDS             complement(14198..15268)
                     /gene="rarD"
                     /locus_tag="P5541_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRV71063.1"
                     /translation="MTEKKAKGARKKETAGERSIRDTSFQVMFSFHKFIIPAVRNIRE
                     EQESMPSLPVEGKRKDETGAGLRSALAAYVTWGLLPLYWKFLAEVPALEILSHRIVWS
                     MLFVSAILLFQGRISEISAIFRSPGTMFLLFLSSLFIGANWLVYIWAVNAGKVLETSL
                     GYYTNPLMNVFLGFLFFRERMERIQWLAILVAAAGVLFQVARYGKFPWVALTLATSFA
                     LYGLIRKQVRVDAIPGLLTETSFLALPALLYLAHAGASSRGLFLAGDPERDLLLLGTG
                     IVTSVPLLWFAFGARRLRLSTLGFLQYVAPTLTFILGTVFFHEPLDMPKVVTFVCIWI
                     ALALYTWGSLRASRRVSGIVEA"
     gene            15396..15812
                     /locus_tag="P5541_03450"
     CDS             15396..15812
                     /locus_tag="P5541_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRV71064.1"
                     /translation="MKLSGKTFMQVHHVFAVSALLLMVTHAVATWVSFGTPTVFIPHF
                     QSLKGFLLFGGRVALPLFILTALTARYSRKIPFWRKIHLLNYFAFIVATSHAVMIGTD
                     FDSILMRTLAWSMAVFAMAVPAVRHFRRKRQDPSRG"
     gene            complement(15809..16423)
                     /locus_tag="P5541_03455"
     CDS             complement(15809..16423)
                     /locus_tag="P5541_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRV71065.1"
                     /translation="MGAGRENRFFPKGRIEALCDGIFAIVMTLLVLDLRAPWFNGRLG
                     RGPFLEFISGIGEPLFNFAVSFVVLSMLWIWHHRQFREILKMDSFALAVNLSFLAVVS
                     LVPFSTSVLSRFMDLWEADLIFHANIMLAGLLLVLNWHYATSDKRLVDAEMDKETVRT
                     GFKSIAIAPMVALLGMILSLAIRGDSTFVYLMVPFMGTLGHRDN"
     gene            16557..17078
                     /locus_tag="P5541_03460"
     CDS             16557..17078
                     /locus_tag="P5541_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRV71066.1"
                     /translation="MSSSLKIPLTLVFSLLLLWVALTGSVKADSILVGLALSLLITFV
                     TWKPLFSGSRHIYVHDPEPLNFRPLKALMALPGFMADLTKASAEVAILAIKPSIQLRP
                     GIVRVHSHLKNKTTIVFLANYITLTPGTLTVDMDISRHDLFIHSLDLDSLDASSLRDE
                     VVSMEGILGGVFE"
     gene            17075..17332
                     /locus_tag="P5541_03465"
     CDS             17075..17332
                     /locus_tag="P5541_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRV71067.1"
                     /translation="MISAILWILLFLAALGFWRVMVGPTVPDRIVAADAVSVILTTML
                     ALMGLVFDNTVFADIAIAFAVLAFADVLIMAKFFEHGELHL"
     gene            17329..17676
                     /gene="mnhG"
                     /locus_tag="P5541_03470"
     CDS             17329..17676
                     /gene="mnhG"
                     /locus_tag="P5541_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRV71068.1"
                     /translation="MTGIVLRNLAFLLMAAGTFFSFASVVGIIRFPDIYTRIHAGTKA
                     LSGGAVLILAGAALIAPSWQMATKLVLVAVFLLATNPIASHAIARACYRHGFKPGVAI
                     VDQYGQIEGGKEK"
     gene            17673..17939
                     /locus_tag="P5541_03475"
     CDS             17673..17939
                     /locus_tag="P5541_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRV71069.1"
                     /translation="MSTVFVAFLYVFLVATAVVASSIGDILSSVIALSVFGILLATAF
                     AIMQAPDVALTQAVINSGIVTALFLVAFSQTEKPECYGEEDDEE"
     gene            17936..18682
                     /locus_tag="P5541_03480"
     CDS             17936..18682
                     /locus_tag="P5541_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRV71070.1"
                     /translation="MNTRPFLFRLAAMVLCVSLGLLLYQGIAAIDQPAHVTGAAEHYL
                     ANTPEETGARNVVAAILFDYRAFDTLGESTVIFTAVCGISMLFSRRKFHRSSRGLSFI
                     ARRGIGLIVPFILMYASAIVLAGHLSPGGGFQGGTIFATVTVLLCVVYGSSFESVHFP
                     PWKKDLMESAGALLFSGVGILGLISGAGFLSNLSAGFPPGQSGSIFSAGAIPVLNVAV
                     ALKVGAGLSTIFYSIIKLLEEGGEHPGRGH"
     gene            18682..19020
                     /locus_tag="P5541_03485"
     CDS             18682..19020
                     /locus_tag="P5541_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRV71071.1"
                     /translation="MMVTMGYTSALIIFCIGLYTIMTRRNLFKTVIGLALMEAAVLLI
                     IVTSGFIPGGTPPIAPFSGNPVNPLPHAFTLTAIVIGASDIALALAFIIKLHRHYKTV
                     DLDRIRGLRG"
     gene            19017..20507
                     /locus_tag="P5541_03490"
     CDS             19017..20507
                     /locus_tag="P5541_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV71072.1"
                     /translation="MTLVLAIVVPLLAAFLIPLFHLARKTLSVSLIGFMGLASISAAL
                     AAAFTGWFSPAVYLLGGWAPDLGIVLVADHLSSAFLLLASIGTAAALACSLEKFRGGP
                     WRYYTIFFLLIASMNGILLTGDLFNLFVFYEIFSVAAYLLVAFSMSWQALEAGLKYLV
                     LGTIGALFILLGTALTFMATGILNMALLAETLPSIPGETLTAISACLLLGLAVKTGAF
                     PVHFWLPDAHSSAQTAVSALLSGVLVKVSIYAIMRVSYLFFLGTGGNVFLAITIIGSI
                     SVLGGHLMALRQDDIKRLLAYSTVAQIGYILIGVGCASAAGAAAAVFHAFNHMLMKMG
                     LFITAGTLAEDKKTREICGLKGLWKSHPLWVSSFTVLSAAIVGIPPMNGFMSKWFIIL
                     SSAGAGTVIPALVIAAGTVISAAYYLRVLSAFISPEDERSESASAAPPRRIRRKGISL
                     ALVTSFALLCLVLGATAFSPISREFFSSIGDTAVDVGAYVELVTGR"
     gene            20504..21904
                     /locus_tag="P5541_03495"
     CDS             20504..21904
                     /locus_tag="P5541_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV71073.1"
                     /translation="MSLSNMFTGMNMPFGAAAFFLFLSTALFGEWYFGSGRDKKRFRI
                     LLAAAAASSTGILSSGNWLLFVLFLEILTLSLWSMISMKDPRAAMVYLLSQLTAAGLI
                     LVGAAGVFSDTGSLAIGPVPEPWKVFFLAGFGIKAAFPLLHFWLPEAHGKAPSPASAL
                     LSGFAVKTGILGLIRAFPEPFTPLVLIGTVMALFGAFMAVFPRDIKRVLAYSTMSHLG
                     FMTTAIGAGATSIAAAYAFSHAIIKGTLFMSAGILEKTFGTRDISALEGIGRSHFATV
                     APLVTGAFFAAGLPGTASYLLKNSVISSLGEAAHWLQPFLLASAGMLASGYLLNITVP
                     LFFKGETEKQAESGKGSGFAISAILILLLVLSTDSFFSGILEPIRSVPETLSGSGKPG
                     SFLATVAAGIIVLLARRRITGIPQQGLPDIRIYLSMEEGPLCALNRASGCCHPGRLNI
                     FVFVALAFLVLLMLIL"
     gene            21917..23371
                     /locus_tag="P5541_03500"
     CDS             21917..23371
                     /locus_tag="P5541_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV71074.1"
                     /translation="MMFETVHQSLRIDNFSLFMSALVVFMTVAVSIYSLGYCEERTFW
                     RRVTFFGAPWAFCLSSCLAVMSGNWFLFTIFVELSSLALFSMILLQDPGAARLYIFSQ
                     LVGAGFLLAGTALLSAETGTISIGPVPGNLLWLFLTGLGLKAAFPVLHFWLPEVHSRA
                     PSPASALLSGFAVKIGIFGLAKLASPETGTFFLATGSAMALYGVGQALLQHDAKRLLA
                     YHTISQLGYIITAFGAGTALGIAAGFFHAVAHGLFKGLLFLSVGTLEKIYGTRDLGHL
                     GGAAREMPVTFMLFLVGALSISGCPGFSGFASKAMIKAALYEAPNPAWYWILQAAGVG
                     TVISFCKVGFFAFVREPVQRPEPMGEQIPGGRKDSFRNLGMLLPAAATVALGVMPGFF
                     TRLGGFYGSNLFGIKNLLPAMVTLALGLFIFRLFRRWFEPGSHDIPDVSDIFATASDL
                     SAPLYGLLFSMNCGLLRFYIAAVIAAGVALFLFL"
     gene            23417..24007
                     /locus_tag="P5541_03505"
     CDS             23417..24007
                     /locus_tag="P5541_03505"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HRV71075.1"
                     /translation="MNRIIVPAGHARAFEVEKGTTFTVIDMEGGQIADLVAFNRDNLS
                     EKFSCSHTRLGLHSVGFKVGDSLRTNLRRPMMEIVTDTVGTHDMLIPACDEQRYRIDY
                     GVEEHRSCVANFEEVLAPWGLERRDIPDPLNIFENADIDADGNLVHLPVISKAGDHII
                     FRPLMNLVCAVSACPMDLNITGGNRITDILVEILGP"
     gene            complement(24216..25709)
                     /locus_tag="P5541_03510"
     CDS             complement(24216..25709)
                     /locus_tag="P5541_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRV71076.1"
                     /translation="MKWNPGSISRALFLGLYALAVFVAPLDAAQLTILHVNDTHGHIW
                     NEEGKGGFDALALLVEEIRREVASNGGDVIFLHAGDVNTGIPESDLQEALCDISLLRE
                     MGCDAMVLGNHEFDNPLALLRFQESFARFPFISANFVDSWGKLAFPPYVIKEFDDLKV
                     AVFGLTTESTARSEPLYLEGGSFLNAVDTARDLVPRLRENADVVIALGHLGWGNVVPG
                     STGSADLARASIKGLDVIIDGHSHTLFDNAVLIGSTLVAQAGAFGKYLGRFDLEIEDG
                     RITDWKWKAIPIDPAGGEDPEITNRLDYYEWVGSRKIDQVIGSTRTLLDGERVKVRSG
                     ETNLTRLVVDAIREVAGADIAVVNAGGIRDSIQAGDIRMRDVMAVLPFQNSLVVMDLS
                     GSDIAEILGSISQIKPGLGGFPHVSGIGARSVEAGKDQITIGGVPLDPAKTYRLATST
                     YLASGADGYDAMIPFSKRAVDIGFTDTDALARYIEDHSPIDEKAGAK"
     gene            complement(25754..26974)
                     /locus_tag="P5541_03515"
     CDS             complement(25754..26974)
                     /locus_tag="P5541_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRV71077.1"
                     /translation="MRNRPMSKIIGIMVVLILAAASAGFGSELLLSVSSDNAEFAFDI
                     FSSIRAKEGNLFFSPYSISSALAMTYGGAAGETASQMEKALRFTGGQELTHRAFSELG
                     ERMAEIQKEGNVELSIANSIWPQSGYPFLKEYLSLLDENYGVEITPLDYAREPEPSRE
                     RINGWVEEITKEKIKELIPQGSIDPLTRMVLVNAIYFKGNWEDQFDPEATEEAPFTLS
                     SGKEVNAPLMSRSGNYKYAELPGFQMIELPYAGRDLSMIVLLPDTDTPLSSLEKKMTL
                     ENFETWKKFLKEERVILFLPKFRITWGTVSLVETLHSLGMVDAFSSSKANFSGMDGRP
                     DWLYITDVLHKAFVDVNEEGTEAAAATAVVVGARSVAAPPVVFRADRPFLFIIQENST
                     GNILFMGRVNEPEK"
     gene            complement(26993..27496)
                     /locus_tag="P5541_03520"
     CDS             complement(26993..27496)
                     /locus_tag="P5541_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71078.1"
                     /translation="MNRQVLVNDLMQKGYSYTITEPEGENFHPGFRPDLTPAEMLEMG
                     VFGGKYMTDCADEFPAWWFENARLCTEKHDPALNLFGVNASKPLSYWRQKGWIHHEDP
                     RGWFQWYCRYHMGRRCPDDLRQISRWKAMTRHIAQLRKYCFAGDLECRKRQRQALLHW
                     AYDSRKI"
     gene            complement(27517..28023)
                     /locus_tag="P5541_03525"
     CDS             complement(27517..28023)
                     /locus_tag="P5541_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV71079.1"
                     /translation="MPLTREKRRGFTLVELLVVLVIIGILAGSLLLVFSRSQARARSA
                     VIIGDLRVLKSAATLYYLDNGSWPATTAAAWTIVRDYIGSDSLVSNPQGKEQEKIYAI
                     RRVGDTAANNPGSVWVVANVNDNSSLGINAAVRAVLTSRQAEFGFVNNAFNPYNNHLI
                     IMMPVYRP"
     gene            28196..28549
                     /locus_tag="P5541_03530"
     CDS             28196..28549
                     /locus_tag="P5541_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HRV71080.1"
                     /translation="MNKENNGSTYTRYNAEDLILRDELAIDRTLLANERTLLAYLRSA
                     VALVLAGFTMIHFAADSHWFWLAGFVSIPVGVFAGMIGVVRFRVMNRIISRIRKQKSE
                     ETGEGDQDTGRTKEP"
BASE COUNT         6380 a         7801 c         7871 g         6711 t
ORIGIN      
        1 aagaacagat aaggtcaact ttttgttact catttaggaa tggtctttac gggctcctga
       61 aggaaaaagg gccacccctc tcgggcggcc cttttcagga ttgttgtttg cggatcgcgg
      121 tttttgtcgg agactctagt ttttgcccgg tttccagatc ttccagacgt tgccgaccag
      181 ttccgggccg ggtttgaggg tatccttgcc tggctgccat cctgagggag tggcttcttt
      241 gcccttgctg gcacggacat gctggaaggc cttgatctgc cgtatcgtct cttcgacgtt
      301 tcttccgacc ggaggggtga ggacctccat gccctggata acaccgtcgg gatcaattat
      361 gaagcggccc ctcacatcta ctccggcgtt ttcatcatag acgccgtaga tcttgccaag
      421 gttgccgccc gcgtcggata tcatggggaa tggaactccg ccttcgacca tctttgagat
      481 ctcgttgtcg ttccagatct tgtggacgaa ctggctgtct acacttacgg agaggacatc
      541 tacaccaagt tcctggaatt ccttgtattt gtcggcgacc gccgacactt cggtagctca
      601 gacgaaggtg aaatcaccgg ggtaaaaaca taggacgacc catttgccca gggagtctga
      661 gagttttacg gatccgaatt tcccctggtg ataaacggga gcctcgaagt caggtgcttt
      721 tcttccaacc atcacagaca tccttgggac ctcctttttt gccgtttccg atttctcctc
      781 aactgcaggt tcagcttcgc ctaccattgc tccagtggga cgagcacaac caatctcctt
      841 ttcttccaag agaaccacct cccgatcttc gaagttaaaa tgaaatagtt attcggattc
      901 tttgcattat tatgatatca cattcttgcg ttctaatgca tgatttctgg tgtcatggga
      961 taatggggag gatacaagga agcaggaaac tggtggctgg agactgggaa agcaggggca
     1021 agggcgtaaa cggtcaacgg attggaagac cagggagaca ggagactgga gactggtggc
     1081 agggaaggtc tttggttctc acaaagaaaa gaaaggtttt ctctgtgtgg cttcaccctc
     1141 tgtgtcccct gtggtgaaaa caggttttgc actgggtccg gtttttggtt ttcacaaaga
     1201 aagtgtttct ctgtggtgaa aggggaggtg ggggtcctgt caggagaact ggcatcagaa
     1261 gagaaatttg ccaccgaaat ggattccagg gatattcttg caggtttcag ggagaggttt
     1321 cacctgctgc gggacgtgat ctacatggac ggcaattccc tggggctgtg cccaaaggat
     1381 tccggagagg cagtcaaaag agcgcttgat gagtgggaat gtctcggcat aaggggctgg
     1441 ctggagggaa atccgccgtg gttcgagatc agcgagagaa ccggggctat ggccgcatcc
     1501 attgtcggag ccgggcctga cgaggttgtc atgaccggaa ccacgacggt gaatctgcat
     1561 tccctcgtgt cgacctttta caggccttca aaagaacttc atgtgatcct ggccgatgaa
     1621 atcaatttcc cgtccgacct ttacgcactg gagagtcaga ccgcgataaa gggttttacc
     1681 cctgaaaagt ctctccgcct ggtgaaatcc tccgatggca ggaccctgga cgaaagagag
     1741 atcatcagcg ccatgggacc cgatgtctgc ctggtcctcc tgccctcggt tctttacaga
     1801 agcggccaac tgctggacat ggaattgctt acaagagaag cacacagccg cggcattctt
     1861 gtcggttttg actgttccca ctccgccgga gcggtgcctc atcattttga tgactgggaa
     1921 gttgattttg ccttctggtg cagttacaag tacatgaacg gaggtccggg atcccccgct
     1981 tttctttacg tcaaccgccg gcacttcggg gccatgcccg cactggctgg ttggttcggc
     2041 tacgaaaagt cgcgccagtt cgaaatgaac agggatttca tcccggcagg gaacgccgga
     2101 gcatggcaga tatcgtcccc ttcgatattc ggctcggcag cgacagccgc ggcactgaag
     2161 gttaccctgg aggcaggcat agaaaggatc cgggcaaaat cgatcgggtt gacttctttc
     2221 tttgtgaggc tcgtggattc ttacctgtcc ggggagcctt accgtttctc cgtcgggaca
     2281 ccacgggatc cggagagaag aagcgggcat atcgctctgg agcatcccga tgaagccatg
     2341 aggataaacg aggcgctgat agcccggggc gtgataccgg attttcgccc gccgaacgtc
     2401 atccggattg ccccggtagc tctttatacc acttaccatg atgtgtggca ggtggcggtg
     2461 catctgaagg agatcatgga cgggcgtgag tacgagagat tctcgaagga aagaaaggcc
     2521 gtatcgtagg gggcaagggc cgtaaattca aaggcgtcaa cggtaaacgg tgtggaaggg
     2581 caagggcgtg agaggttaga cgtgagaagt gagacgaaaa aaccaaattc aaaggcgtaa
     2641 acggtgaacg gtttaaagag cgagacgtca ggaaactgga ggcagggaag gtctttggtt
     2701 ctcacaaaaa aaagaagggt tttctctgcg cgggtccacc ctctgcgacc cctgtggtga
     2761 aagggccgta agtcgtaagt cgtaagtcgt gaatcgaatg gaacaagagc gtgagacgtg
     2821 agacgtgaga cgaatggaaa aacaagggcg aaagccttaa atccaactgc gtgaacggtc
     2881 aacggtaaac ggtaaacggt gcgcaaaggc aagagcgtaa gaagttagac gtaagaagtg
     2941 agacgaatgg aaaaacaagg cgtcaggatg caggagacgg gagactggaa gggcaagggc
     3001 tttgtcattc tgagcgtggc gaagaatccg ggttggtttt taaggttttc ctgcctcctg
     3061 tatccagtct cctgcttcca ccgctttcac gatttacgat tcacgattaa cggctttcat
     3121 atttactcag ccactccagg caggtacgga cgccgaagcc tgtggcaccc ttgcagtagg
     3181 aaccgtattc ttcgtcggag ttatctactc ccgctatgtc catgtgtagc catttgatcc
     3241 cctcgtcgac gaattctctc aggaacattg cagccgttat ggcaccgccg taccttccgc
     3301 cggagttgac gatatcggcg atctcggagc ggatcttctt cttaagcctt tcgtcgtcca
     3361 tggggagttc ccacaggcgt tcgccggtgc tgcgcgagac ttccatgaag gatgacgcaa
     3421 gttccacatc gttggtgaat attcccgcag tgtacccgcc cagggctacc acgcaggctc
     3481 ctgtaagcgt cgccatgtcc acgatgacgg acggctcctg tttcgaggca taggcaagaa
     3541 catccccaag tactacccgc ccctcggcat cggtgttatc gacctccacg gtcttgccgt
     3601 tgaacatcct gattatgtcg tcgggcctgt atgccccacc tcccggcatg ttctcggccg
     3661 cgccgatgaa gccgtggacc tccagtgacg gtttgagttc cgatacggct ttcatgatgg
     3721 cgatcacgtt gcaggcaccg gtcttgtcgc ctttcatgct cctcatgaag tcgcccggtt
     3781 tgatgttgag tccgccgctg tcgaaggtaa ggcccttgcc tacaaaagca acctttcctg
     3841 tggcatttcc cgacggcctg tagaccatgt gcacgagcct gggcgcattg tgggagccac
     3901 cccctacggc cagtatcccg ttgaggccga gatctttcat cttcttctcg tcgaggacga
     3961 cgcactcaag acctgattcc tccgacattt ttcgggcttt ctctgccagg gtagcggggc
     4021 tgatcacgtt gccgggttcg ttggccaggt ctctggcgaa gttctgggca gccgccgtga
     4081 caagaccctt ttccaccgag gctgcatctc ctccgtgtat atagagttcc tccagtttaa
     4141 gtggtttttc tgatgtttcc tctacgctct tgtacctgtc gaaacggtac tctcccagca
     4201 gagcgccttc cactattgcc tttgctgctt cttctttagc tccgtcgcaa ggcaggacca
     4261 tcattttcgt gaaacgatct ttccccgcac gcctgacggc ggaagcggct cctcttctcc
     4321 agttatcggg agcacatttt tctcttgacc caagaccgac aaggtagacg cttttcacct
     4381 ggcctcctgg aacgtttact gcaaggaagg aatcggcctt cgcccggaaa tcgaccctgg
     4441 atacggcttc ggcaagagtc gggcgaatgt cttctgccgt tttttccagg ggatccattt
     4501 cgtcttcgaa aagaaagaga accagaacat cgccattcca ctctgccggg gagagtccgt
     4561 atgtttctat tttcatcata tgcacctcca aaactggttt ccacacccgg aaacaatagc
     4621 atataatcgt caaggggtga cagccattcc gggagacctt ttcgtcggtt tcgatatcgg
     4681 atccgcggca ctccactgct cggtcctggg caatgacagt tcagtcctgt tctctccgtc
     4741 ccccattctg cacctcggca aacccttcga ttcgctccat catctctggc tcgaaattct
     4801 tgacagattc cgcgaagacc gcatcgcttc tacatctttt acaggtatgg ggtcgacctg
     4861 ttttccggag gtgttccccg gagtcatgag ggaacatgac agtctctgca ttgttcgggg
     4921 agcttcgatc acaagccctg gtacggagat cgtcatccac ctgggcgcca gggatgcctg
     4981 ttttttccgc gtggcgggag aagggaaacg cccgatactg atcgactgga gcaccagcag
     5041 caagtgcggg gcaggctccg ggacgctgct ggagaagcag gtccgacgcc ttttcccttt
     5101 tggtggggat aacacggagg atttcggcga agccctggca aaggccgaag agagcgcccg
     5161 gggattcgac ggggcaggag cgtataacgc gagatgcgga gtggttctcc agtcggacct
     5221 tattcatgac cagaatgatg gcgttgagag gggcctgatc atggcacggc tcttccggac
     5281 gatagcctcc agcatagtac aggacatcat cggcaacagg gacctcgagg ccgggagaag
     5341 ggcatgcgtc acgggatggc ttgcggggtg ctctcccatc ctgaggcata ttggggaaat
     5401 aacatctctc gacgtctttc gcgccccaga cttcgcgaac gtcggagcca tcggcgcggc
     5461 tgccatagcc ctggagaggg gcaacaactc cgtcgcggac atggggatgc tggaggaggt
     5521 ctcatccttt gcccgatcta aaagacaata tatgccttcc ctgtcaaact cggtttcaag
     5581 ggtttttatc cacaaatccc ccagaacggc cagccgggat aactcacctg cttccagcgg
     5641 agccgtactc ggcgtagacg gaggatccac aaccaccaag gctgttgtca tatcactgga
     5701 tacgggggag atgctcggcg gtgaatacgt tcccactcac ggcaaccccc tcggggctct
     5761 cgctattgtc atggaccgcc tttcatcttc cctttgttcc cgcaccatag aggccgtctg
     5821 taccacgggt tccgcacgcg ttcttttcga gaaggtcctt tccagccctt cactgaggaa
     5881 acggctggag ggtgagggtt tcaaggtcca cgacggagcc gtcgacgaga taacctgtca
     5941 tgcggaaggc ataaggtcca tggactccgg tgtcgatacg atcttcgaga tcggcggaca
     6001 ggacatgaag ttcacaacct tcaggaggga tggcctcaag gctacggacg aggtcgacga
     6061 ggccaggatg aactattcct gccaggccgg agccgggcag acccttgaga atatggccgc
     6121 gatcatagga ctggacgtga agagttccct ccaggaagag gctctcaagg cggacagggt
     6181 ccccgcgata gacgctacct gcggagtttt catggagatg gaggagcaca ggcttatcgc
     6241 cgaaaaccac accgtgcccg agatcgccgc ggcgattgtt cgcgccacgg catcgagtta
     6301 ctttaacaga tttgtcggag gaggaaggca cgtcaaggag aagtgctcat gccagggtgg
     6361 acccgccctg gggaaggcat tcctggcagc gatggctgac gttacgggca gggagatcca
     6421 cgcgtacccg aaccgcgaac tgatgggagc catgggagcg gcgatcctgg caaggagggc
     6481 tgtccttaac tcgagaatgg agggtatccc ctcggcctcc gccttcaggg ggtgggacgc
     6541 ggcacaaaaa ccttttgcca gttccgagaa actctgccgg gaccacttcg gcaaggattc
     6601 ctgtggtaaa aggaactgcc tgctcaagat cttttccatc gggggggagg agatcgtttc
     6661 cggggggttc tgcccactgg gaaacagcga gggagccggt aaaacaagac cggatcacgt
     6721 cgcggtgttt cactccctgc tggaaaaaca tttcgaggga atcaggttcg acgaacttta
     6781 cgccgccgag gatgaccaac gcccccgaat agggatcaga aggtgttcca ccacgatcgg
     6841 ggggatgggt gtctgggcat cggccctttt ttcttctctc gggtttctcc cgatcctttc
     6901 accggtgtcg gacgagagga tctcgagact cggccttagc cactcctcca ccgatttctg
     6961 tgtggcaatg aaaatttccg ccggacacac cgctcttatg gctggagaca aaagggttga
     7021 ccatgttttt atcccatcct tcgtggacag ggcccgggac gaggagccgc ctcacacgtt
     7081 ctgcatatat accgaagccg agggtttttt gccgggagac gatctggggc tttcggcctc
     7141 ccgtgttatc cgacccgtct ggcacctggg agacagggag cagatggcaa ggaccctttg
     7201 cgaggaactt tcactggccg ggtacgatat gcctttgagc aagatcatgt ccgcgttcca
     7261 gaatgccgac gaagcaaaaa gatctttcga tgccgagatc gcaagggttg gagaccggtt
     7321 cctggaatca atcgagtcaa aggacgagcc gggctacgta gggctgggaa gggactacgt
     7381 cgtccttgac ccggcggcat cctccgatac ggggagaatg ttttcgaccc ttcgcgggat
     7441 gaactatata ccccaggttt ttctcaccca cctgttcgac tccgtgcctg tcgacgatct
     7501 ggtctccaac gagtactggg agcagagttc ctccatcctg aaggcatcaa tatttactgc
     7561 ccgtcacgag cggctttacc cgatacggca gatgaacttc gcctgcggtc ctgactccat
     7621 caagttcctg atggaggacg agatattccg gagggcagaa aaacccttcc tgcatctttt
     7681 gaccgacgcc cagaccaaca acgcgccatt cgttacgaga gcagaagcct tcgagagggt
     7741 cgtggcaaaa cgacaccctt cgcaaaaacc tttttcggcc ttctccttta tccgcgaaaa
     7801 gggcaatgaa aataccggaa gaaggtgggt aataccatcc atggggaacg cgagccttct
     7861 cggggccgct gtggggcgtt cctggggtct tgacacagtg gtggcgccca ctgccactgc
     7921 cgatgcaaga gaggccgctg aaaggcttat atcgacggag acatgtttcc ccctgaaggg
     7981 agtcatagga gacgttattt cgtacctgga aaaagaggct cgaaagaacg gcatagaggc
     8041 ggtaaactca aggtacctcg ttttccttcc cacaaccagc ggcccatgca ggttcggcaa
     8101 gtacgccgag gtgatggggc ttctgctgga cagccttgga tttaccggtg tcccggtcct
     8161 ctctccatcg tcccataacg gctatcttga ttcaaagacc ctgggtcttc cctggtcgcc
     8221 cgtcgcccgc acaggttttt tgagggacat tttcaattcc ataatcgcat cggatcttta
     8281 tgatgatctg gtcctcaggt ttcgtcccta cgcgaccgat agtgagattt tccgcaaaaa
     8341 ggcctctgaa aggctcgctg tgctcgaaag aaccatcgaa tcttccggag cctcgctgag
     8401 ggcgatctcc gggtggggag agaaaacggc tcaagaattt gctgcccttt cctctcccgg
     8461 gatcccccgg ttccccctgg tgctctacct gggggagata tacatgcgtc agcacgacct
     8521 gtacactcga aacgcaatcg aaatgctgga gagaacggga ctggaacttg tgaggggacc
     8581 ggtttcggaa tggctgcggt acgtcgtgca tatctccagg aggaaaaacc cgcgcctcga
     8641 gtacattgcc gcgtcgatgt acatgaattt cgcgcagaga tcatcggaca aggtcgcgag
     8701 gggacccctt gccgacagaa aggtgcttcc ccacccggaa gagaccatcc cctggatcga
     8761 aaaagccggg atctaccatg gcgatatcct tggagaatcg ggaatatccg tcggtatttt
     8821 caacgccttt ctgaagggag atatggcctt ccacagcgga aaccccgtct gcggaatctt
     8881 tcatgtcggt cctttcacct gtatgcagga gggagttgcc acagccagga tcaggggcat
     8941 gctcaaggcc gctagaagaa aggaacccgg catgatcgtc cccgttatga atgcctcttt
     9001 cggggagtct cccaacccca acctggaggc ggaggttgcc gctttccgtg aacagtgcct
     9061 cctgctccgc gattctctca tgaggccatc acgaaccgag aaaagggcat gaaccagaca
     9121 ctcccttgcg ccggatttgg ggagatgtta aaaggaacag gtgccatggg ccagtgttag
     9181 ttgctcctat tgcaacacat agcataaatg ctataatcac cggagatgaa gtaatgtgga
     9241 aaattgtttt tcttgacaaa gccgtagaag aggaattcca ggctttgacc ctcaaggcaa
     9301 aggcgaaatt cgacaggatt gaggatttga tacgcctcta tggtctcgaa aatattggaa
     9361 ggccatatgt ggcccctgtc gctgataaaa tctgggaaat tcgattaaaa acatccgact
     9421 ccaatgccag aggtctctac gcaacaaaga ggggaaaagt aatagtaata cttcgtttct
     9481 ttgaaaagaa atcccaaaaa atcccgaaaa atgagttttc tcttgcaaaa gaaaggttaa
     9541 aaaaaatttc ggaccaggat tccaggttgg gagggtagta acaaatgcct acagtgccat
     9601 atagcaaaat aaaaaaagaa ctacatgaag atccggactt tctacgagaa tataactctc
     9661 tggcagatga gttccagata gccgaggccc tgatacgggc acgaacggct gcaggtttga
     9721 cacaaaaaga ggtcgccgaa agaatgggaa caacccagtc tgcagtagcc aggatggaat
     9781 caggacgttc tgtatcacta aaaagcctga aaaaatacgc caggagcaca cactccagga
     9841 tcaccctgaa aattgagtaa attttcagag gaggtgcaag cattgtcttt cctggaactt
     9901 gcaggcaaga gacggagcgt gcgaaacttt ctgccggatc cggtcccgag agagaccatt
     9961 gaaaggtgcc tcgaggccgc aaggctggca ccatcggcct gcaattcgca gccatggagt
    10021 tttctggtcg ccgataaacc cgaaacatcc gccaggatcg ccgaggccgc cttctctgga
    10081 ctttattcga tgaacacctt cgcgaaaaag gccccggtac atatagtcgt gatccgcgag
    10141 aacccaaagc ccgctgccat gttcgggggt tttatgcggg ggatggattt caccctcgta
    10201 gatatcggga tcgcctgtga acacatcgtt ctccaggcgg ccgaggagaa tatcggaagc
    10261 tgttttttcg gatggttcga cgaaaagaag gtaaaaaagc tcctggctct cccgaggaga
    10321 tccagggtgg acctcatcat cagcctcggt tttaccgacc gccccctggc ggcaccaaag
    10381 caacggaaaa ctctggaaga gatacgcagg tacgtttctg tcgtataatt cccggtgaag
    10441 acaaacaatc tgcagggagg aaaccaaaat gccaaggaaa atcctcgttt gcgtcgaaat
    10501 gggagatctc gcggccaaac tcgtagacta cgggaactcg cttgcccacc gcctggatgt
    10561 ggaagtctcc tttctccatg tcctccccaa cccgcacatg tggaagggtt acggtacctg
    10621 gatccagccc gatgtgacca aagatgcaaa ggaaagcgcc cgcaagaaga tccagtacct
    10681 catcaaaaaa gccgaggaga aacaccccga gcgtggcgaa catgaacacc agatcctttt
    10741 cgggcaggga gacacctcgg aaataatcat caatacggcc aaagagggag gattcaacct
    10801 cattgtgata gggttcaagg gaagcagcgg gatcgagcac atgatggtgg gaagcaccac
    10861 cactaacgtc acacgctatt cccactgctc cgtcctcgta tatcgcccgg gagaagaaaa
    10921 ccctatctag gccgccatga cggaaaaact cgattttgat cttttcggag atgccgcttg
    10981 atacgctgca gcggcatctt cctggttctg ggcggcaagc aggtctttga agggttatcc
    11041 ctctctgtac ccaaagggac gaggcttggg atcgtcggcg acaacggtgc cgggaagacg
    11101 acccttttca ggctgatcaa cggagaggta tcacccgaca ggggcgatgt ttcgatccct
    11161 tcatcttcca ggctgggtta tcttccccag gaccttgtgg agatcgggag tgacaggctc
    11221 atggattttc tcaaggatcg ggcggggata tcgtccctgg agagggagat cgcccggata
    11281 ggtgaggacc tttccgtgcc cgggctggag gagcagaaac gcagagggct gatcgagagg
    11341 catgaaaggg ctgtcagaag atttgaatct gctgaaggat acggtttcga ggccagagca
    11401 tcgaagatcc ttaaaggact ggggttcagg gacggtgacg gggccagaaa ctgcacggag
    11461 ttttccggag ggtggaagat gcgggcctcc ctggcatcaa tcttgctttg ccgcccggac
    11521 attcttcttc tggacgagcc tacgaaccat ctcgatacgg agagcatgga atggctcgag
    11581 agttatcttc tgtcctttcc cggcacactg ctggcgatct cccatgaccg caggttcctg
    11641 gacaggctga caaccagcac ggcagagctt tccggaggga agacaaggct ctactcaggc
    11701 aatttttcca gttacgtgga aaagagggag aaggaacttg agatagagga aaaaagccgg
    11761 aaacgccagg caaaagagac cgcaagaaca atggagttta tcgagaggtt ccggtacaag
    11821 gccaccaagg caacccaggt ccagagcagg atcagaaaac ttgacaagac agtaactctt
    11881 gaggcccagt ccgcggctcg aagggtatcg atgagattcc ccgagtgccc gaggagcggc
    11941 ctggaagtga tccgcgccga ggatgtagcc cactcctacg aaggaaagac cgttttctcc
    12001 ggggtggacc tgacgatacg caggggggag aggaccgccc tggtcggcat caacggagcc
    12061 ggaaaatcga cgcttacacg gcttttggcc ggaaaggaaa acccgtcgga aggcacggtc
    12121 aggatggggc acagggtcat tagtgctttc ttttcgcagg aaagcgccga caatctggat
    12181 tacgggcact ccgtctggga tgagatcaga aaagctcctt ccgtctttga cgaacctcaa
    12241 agaagaaacc ttctgggctc ttttcttttt tcggggaacg acatagaaaa acccgttggt
    12301 gtgctttccg gaggggaaaa atcccggcta gccctgctga aaatattgtt gtcgaagacg
    12361 aacctgctta ttctcgacga accgacgaat cacctcgata tggccaccag agatcttttc
    12421 caggaagccc ttctcgggta cggcggcaca atcgtaattg tttcccacga cagggatttc
    12481 ctggacaacc ttgtggacag ggtgatcgag ataagggacg ggaaaatatt tgattatccc
    12541 ggcaactact cctggttcat agagaagagg gaagcggcca ggatcgctgc gggcagggac
    12601 gaaacctcga aaaccgggga cctgcctcag gacggcgggc agaaggaaaa gaaaaggctt
    12661 gaggctgaga caaggaacag gatctaccgc gaaaggaaaa aattcctgga caaactctct
    12721 cccatagagg agggcatcga gagggccgaa aaagagatcg cttccatcga tacgtcgctt
    12781 gccgacccgc tagttctttc taactcggaa aaggtcaaaa ggctgatggt ggaacgttcc
    12841 cgcctggaaa aagagagcgg cgccatgatg cagaagtggg aggagataat gtcttccatg
    12901 gaggctgcct gcccggagga gtaggtcctg atcaggattt ttactgtttc ttgacctccg
    12961 ggtcgccttt accgcaggga gcgacccatt tgccgcagaa cgagcatttt tcactgtcac
    13021 aggggatcct tgccccgcag aagggacagg cttttttccg gcagcaattg gagagatgca
    13081 tgaaaacccc gagaaagagg agagcaagcc ctatccacct gagaggggtc accgccagac
    13141 cggtgagcag aacggcaaaa cccgccacga taatgtaaag cggagtaccc ttgatccccc
    13201 ttgtcagcaa tcaacacacc cgcctttttt atcttctgtt tcttaacaca caatacaccc
    13261 tattctactt ttttatttgc ttttctccaa cagaggttcg gggtcgacca ttttcccctg
    13321 gaggctcaaa ccgaaatgga ggtgcggccc ggtgaccctt cccgtcctcc ccgcaagggc
    13381 aattacatct ccggccgata ccaggtcacc ctcttcggca agtctcctgg agaggtgcat
    13441 gtagacggtc actaccccgt tgccgtggtc tacatagaga cactctccgg cgtagtaatg
    13501 atcggcggac aggacaacag tcccgtctgc ggcggcgagt atcgggtcgc cttcgcgggc
    13561 cctgtagtcg acacccctgt gaggagccct tggctggccg ttaagtatcc tcctgaaccc
    13621 gtaggggctg gtgatgacgc cgggtacagg acgggtgaaa gggatggtcc acttcctggc
    13681 agatgtcatt gtctcaaggg ccttcaggga ttcttccctc tctattttga tacgctccag
    13741 gaccccttct ggcggcgtga ccattcctgc gggaagggac agtctctgga ccgggaattc
    13801 cctgtggatc acatcgatgt ggcgtgtaat gctaaagctc tccccctcga taacagatga
    13861 gacctcaagg accccgggcc ccggtttcga gcttcccaca tcggttccca caagcaccat
    13921 cgcctcgcac ccccccgcaa ggatctccac ttcgggagaa acagttgctc cctcccacgt
    13981 aattatcagg tcttcgatta ttttcggggc tgagacataa accaggaatg ggctgccgat
    14041 cctggtctcc cctggcgcga caaggttcag aggccggtca gaaagggcct ctgcgacaaa
    14101 aacaagggac ataaacagga tcatggctgt ggcaatagca atgcctgttt tcgaacgaag
    14161 aagaactttc attcctgaac ctccaataaa tggtatttca agcctcaact atcccggaaa
    14221 cccgcctgga ggctcgcagg ctcccccagg tataaagcgc gagggctatc cagatgcaca
    14281 cgaaggtgac cacctttggc atatcgagag gttcgtggaa aaaaaccgtc ccgagaatga
    14341 aggttagcgt cggggcgaca tattgcagga accccagggt ggaaagacgg agccttctcg
    14401 ctccgaaggc aaaccagaga agcggaaccg atgtgacaat gcccgtaccc agaagcagga
    14461 gatccctctc cgggtctccc gccaggaaaa gccctcttga agaggctccg gcatgtgcca
    14521 gatacagaag ggccggtagc gcaagaaaag atgtttcggt gagaaggccg ggaatagcgt
    14581 caacccggac ctgttttctt atcagaccat agagggcaaa ggatgtggcc agtgtcaggg
    14641 caacccacgg aaatttgccg tatcttgcta cctggaacag cacgccagcc gcagcgacaa
    14701 ggattgccaa ccactggata cgttccatcc tttccctgaa aaataaaaaa ccgaggaata
    14761 cgttcattag agggttggta tagtatccca ggctcgtctc gaggaccttt cccgcattta
    14821 cggcccagat gtagaccagc cagttggctc ctatgaacag actgctcaaa aaaagcagga
    14881 acatggtccc cggggaccgg aatatcgcgg aaatctcact gatcctcccc tggaacagaa
    14941 gaatggccga aacaaaaagc atcgaccaga ctatcctgtg ggaaagtatc tccagggcag
    15001 gcacttccgc aaggaatttc cagtaaaggg gcagaagacc ccatgtcacg tacgctgcaa
    15061 gggccgacct aagcccggct cccgtctcat cttttctctt tccttctacc ggcaatgacg
    15121 gcatagactc ctgttcctcc cgaatgttgc gaactgctgg tatgataaac ttgtggaacg
    15181 aaaacattac ctgaaaagag gtgtccctga tggatcgttc ccccgctgtt tctttctttc
    15241 tggcaccttt ggccttcttt tcggtcatta cagcctacca tctatagacg atcccgtccc
    15301 ccgatcccgt atacgccacg atacgcgcta caggaatatt ggcctacgcc tcttcttttt
    15361 tcgccatcgt gacccccgcg tttccaaagg agtcgatgaa actatcgggg aagaccttca
    15421 tgcaggtcca ccatgttttc gccgtgtcgg cgctgctcct gatggtgaca cacgccgtgg
    15481 ccacatgggt ctcctttggc actccgacag tttttatacc tcatttccag agcctgaagg
    15541 ggtttttgct cttcggtgga cgagtggctc tgcccctctt catactgaca gccctgaccg
    15601 caaggtacag ccgaaagatc cccttctgga ggaagataca cctgctcaac tacttcgctt
    15661 tcattgtggc aacatctcac gcggtaatga tagggaccga ttttgattcc attcttatgc
    15721 gaactctcgc ctggtcgatg gctgtcttcg caatggcagt acctgccgtg aggcattttc
    15781 gaaggaagcg ccaggatccc tcccggggtt aattgtctct gtgacccaag gtccccatga
    15841 aaggcaccat aagatagacg aatgtgctgt ctcccctgat ggcaagggat agaatcatcc
    15901 ccagaagcgc taccatcggg gctatggcga tcgatttgaa ccctgttctg actgtttctt
    15961 tatccatctc cgcgtcgaca agtcttttgt cactggtcgc gtagtgccag ttaagcacaa
    16021 gaaggaggcc agccagcatt atgttggcat ggaagatcag gtcggcttcc cagagatcca
    16081 tgaaacggct gaggacggat gtggaaaagg ggaccagact taccacagca aggaaggaaa
    16141 gattgaccgc gagagcaaag gagtccattt tcagtatctc cctgaactgc ctgtggtgcc
    16201 atatccagag catggaaagc accacaaaac tgacggcaaa attgaagagg ggttccccga
    16261 taccagatat gaactcaaga aaaggaccac gccccaggcg cccattgaac cagggagccc
    16321 tgaggtccaa aacgagcagg gtcattacta tcgcgaatat tccatcacac agggcctcga
    16381 tacgcccctt ggggaaaaac ctgttttctc tcccggcacc catacctgtt actccttccc
    16441 ggggctctgg aagcgcccaa ttcaaattat atccgtactt tcggtaatat gcccgagatc
    16501 ttataatcga taatgcagat tcaaaggaac agatatggaa gggaggctat tccgccttga
    16561 gttcatcctt gaaaatccct cttacacttg ttttttccct gcttctgctg tgggtagcgc
    16621 tcacgggatc tgtcaaggca gattctatcc tggttggtct ggctctaagt ctgctgatta
    16681 cttttgtgac atggaaacca ctattttcgg gttccagaca catttatgtc cacgacccgg
    16741 aaccccttaa cttccgtccg ctgaaagccc tgatggcact gcccgggttt atggcggatc
    16801 tgacaaaagc atctgccgag gtggcgatcc ttgccatcaa gccatccata cagttgagac
    16861 ctggcatagt aagggtccac tcacacctca agaacaagac caccattgta tttttggcca
    16921 actacataac cctgaccccc gggactctga cggtagatat ggacatttca cgccacgatc
    16981 tctttattca ctctctggac ctggacagcc tcgacgcttc ttccctcagg gatgaagtgg
    17041 tatccatgga aggcatcctg ggaggtgtgt ttgagtgatt agcgccatat tatggatact
    17101 gctctttctc gccgccctgg gcttctggcg ggtcatggtg gggccaacgg tacctgacag
    17161 aatcgtcgcc gcagacgccg tatcggtgat attgacgaca atgctggctc ttatgggcct
    17221 tgtttttgat aatacggtct ttgcggatat cgccatcgca ttcgctgttc tggccttcgc
    17281 ggatgttctt atcatggcga aattcttcga acacggggaa ctccacctat gaccgggatc
    17341 gtattgagga acctggcttt tcttctgatg gctgcaggga cttttttctc atttgcctcg
    17401 gtggtgggga tcatccgttt tcccgacatc tacacccgta ttcatgccgg taccaaggct
    17461 ctttcggggg gtgcggtgct tattctcgcg ggggcagccc tcatcgcccc ctcgtggcag
    17521 atggccacaa aactggtact tgtagcggtt ttcctcctgg cgacaaaccc catcgcatcc
    17581 catgccattg cgagggcttg ttacaggcac ggcttcaaac ccggtgtcgc cattgtggac
    17641 caatacgggc agatagaagg cggtaaagag aaatgagcac ggttttcgta gctttcctct
    17701 atgtttttct tgttgcaaca gctgtagtcg catccagtat cggcgacata ctgagttcgg
    17761 tcattgcctt gtcggtcttc gggatacttc ttgccactgc tttcgcaatt atgcaggctc
    17821 cggacgtggc ccttacacag gccgtgatca attcaggaat agtcactgcc cttttccttg
    17881 tggcattctc acaaactgaa aaaccggagt gttacgggga ggaggatgat gaggaatgaa
    17941 cacccgtccg ttccttttcc gtctggcggc aatggtcctg tgcgtttccc tggggctgct
    18001 tctttaccag gggatcgcgg ccattgacca gcccgcgcat gtgaccgggg cagcagaaca
    18061 ctaccttgca aacaccccgg aggaaaccgg agcaaggaac gttgtggcgg caattctttt
    18121 cgattacagg gctttcgaca ccctcggcga atcgacggtt atctttactg ccgtatgcgg
    18181 catttcaatg cttttctcga ggaggaagtt ccaccgttcc tcgagaggac tctcattcat
    18241 agcaagaagg gggataggtc tgatagttcc ctttattctg atgtatgcct ccgctatcgt
    18301 tcttgcgggg catctctctc cggggggagg tttccaggga ggcacaatat tcgccaccgt
    18361 cactgttctc ttgtgcgtgg tctacggctc cagtttcgaa tccgtacatt ttcccccctg
    18421 gaaaaaggac ctcatggagt ccgccggcgc gctgctcttc tccggagtgg ggattctggg
    18481 gcttatttca ggtgcggggt ttctttcgaa cctttcggcg ggttttcctc ctggccaatc
    18541 ggggtccatc ttctccgccg gcgccatacc ggtccttaat gtcgccgtgg ccctcaaggt
    18601 tggcgctggg ctttcgacga tcttttacag catcatcaaa ttgcttgaag agggcggaga
    18661 acatcccgga agggggcact gatgatggtg accatgggct acacttccgc tttaataatt
    18721 ttttgtattg gcctttacac catcatgaca aggagaaacc tcttcaagac agtgatcggc
    18781 cttgccctga tggaagcagc ggtacttctg atcatcgtca cctcggggtt tatacccggt
    18841 gggaccccac cgatcgcccc tttttccggc aaccccgtta acccgctgcc tcacgctttt
    18901 acccttacgg ccatagttat aggggcgagc gatattgccc tcgcgctggc attcataatc
    18961 aagctccaca ggcactacaa gaccgtcgac cttgacagga tcagggggtt gcgcggatga
    19021 ccctcgtcct ggccatagtg gtaccccttc ttgcggcatt cctgatacct cttttccacc
    19081 ttgcaaggaa gaccctttcg gtctccctga taggtttcat ggggctggcc tcgatatcgg
    19141 ctgcgctggc tgccgcattc acgggctggt tctcccctgc cgtctacctg ttggggggct
    19201 gggctccaga tctggggata gttctcgtag ctgaccacct ctcctcggcc ttcctgttgc
    19261 tcgcatccat cgggacagcc gccgctctgg cctgctccct ggaaaagttc cgcgggggcc
    19321 cgtggaggta ctatacgatc ttcttcctgc tgatcgcgtc gatgaacgga attctgctca
    19381 cgggggacct tttcaacctt tttgtcttct acgaaatttt ttccgtggcg gcctatctgc
    19441 tggtggcctt ctccatgtca tggcaggccc tcgaggcggg gctcaagtac cttgtcctgg
    19501 ggaccatagg ggcccttttc atcctccttg gtacggccct cactttcatg gcaacgggaa
    19561 ttctcaacat ggctcttctg gcggagacgc ttccatccat tcccggggag accctgacgg
    19621 ccatctctgc ctgcctcttg ctgggacttg cggtcaagac cggcgccttc cccgtacact
    19681 tctggctccc cgatgcccat tcctctgccc aaaccgccgt aagcgccctt ctttcgggcg
    19741 tcctcgtgaa ggtctccata tatgccatta tgcgtgttag ttaccttttc ttcctgggga
    19801 ccggaggaaa tgttttcctg gcgatcacga taattggttc tatttccgtc cttgggggac
    19861 acctgatggc actgaggcag gatgacatca agaggctgct ggcctattcc acggtggccc
    19921 agatcggtta catccttatc ggcgtagggt gcgccagcgc ggccggggca gcggcggcgg
    19981 tcttccacgc cttcaatcac atgcttatga agatgggact tttcattacc gcaggaaccc
    20041 ttgcggaaga caagaagacc agggagattt gcgggcttaa gggcctatgg aagagccacc
    20101 ccctatgggt ttcgtccttc accgttttgt cggccgccat tgttggcata ccgcctatga
    20161 acgggttcat gagcaagtgg ttcatcattc tctcctctgc cggggccgga acggtgatcc
    20221 ccgcgctggt aattgcggca ggcacagtga tctccgcagc atactacctg cgggtccttt
    20281 ctgccttcat ttcgccggaa gatgaacgat cagaaagcgc gtcagcagca cccccccgaa
    20341 ggattcgaag aaaggggatt tcactggcat tggtaaccag ttttgccctt ttatgcctgg
    20401 tgctgggagc aacggccttc tcccccattt cgcgggagtt cttctcttca atcggcgata
    20461 ctgcggtgga tgtgggggct tatgtggaac tggtgacagg cagatgagcc tttcgaatat
    20521 gttcacgggc atgaacatgc ccttcggtgc cgcagcattt tttcttttcc tgtcgacggc
    20581 gcttttcggg gaatggtatt tcggcagcgg cagggataaa aagcgattca ggatccttct
    20641 ggcagccgcg gcggcgtctt ccacaggaat attgtcatca ggtaactggc ttcttttcgt
    20701 cctgttcctg gagatcctta ccctgtctct gtggagcatg atctccatga aggatccccg
    20761 ggccgccatg gtataccttc tctcccagct gaccgccgcg gggctgatcc tggtgggggc
    20821 cgccggggtc ttctcggaca cggggtccct tgctatcggc cctgtgcctg aaccctggaa
    20881 ggttttcttc cttgcgggtt tcgggatcaa ggcagccttc cccctgcttc acttctggct
    20941 tcccgaggcc cacgggaagg ccccttctcc cgcaagcgcc ctgctttcag gtttcgcagt
    21001 caagacaggt atactggggc ttatcagggc tttcccggaa cccttcaccc cccttgtgct
    21061 gatcggcaca gtcatggctt tattcggagc ctttatggcg gttttcccaa gggacatcaa
    21121 gagggtactc gcgtactcaa ccatgagcca cctgggcttc atgaccaccg ccatcggtgc
    21181 cggggcaact tcgatagcgg cggcctacgc tttctctcat gccatcataa agggaaccct
    21241 cttcatgtct gcaggcatcc tggagaaaac tttcgggacc agggacatat cagccctgga
    21301 aggaatcgga agaagtcact ttgccaccgt cgcgcccctg gtgaccgggg ctttttttgc
    21361 tgccgggctg ccgggaaccg cttcatatct tctgaagaat tccgtcatct catctcttgg
    21421 cgaggcagca cattggttgc agcccttcct gctggcttcg gccggaatgc tggcgtcggg
    21481 ctacctcctg aacatcacgg tccccctttt ctttaaaggg gaaacagaaa aacaggcaga
    21541 gtcgggaaaa ggttcgggtt ttgccatatc ggcgatcctg atccttctgt tggtcctctc
    21601 gacggattct tttttttccg ggatcctgga accgatccga agcgtcccgg agactctttc
    21661 cgggtcggga aagccggggt cgtttctggc aaccgtcgca gcgggcatca ttgttctgct
    21721 tgcaaggcgg aggatcacgg gaatacctca acagggactc cccgacataa ggatatacct
    21781 gtcgatggaa gaagggcctt tgtgtgcttt aaacagggct tcggggtgct gccacccggg
    21841 ccgcctcaat atcttcgttt ttgtggccct ggcttttctg gtcctcctga tgctgattct
    21901 ctaggaagag gatgaaatga tgttcgaaac ggttcatcaa tcgttgcgga tcgacaattt
    21961 ttctctgttt atgtcggccc tggtcgtttt catgacggta gccgtctcca tttacagcct
    22021 cggttactgc gaagaaagga ctttctggag gagagtaacc ttctttgggg ctccgtgggc
    22081 tttctgcctc tcctcatgcc ttgcggtaat gtccggcaac tggtttctct ttacgatatt
    22141 tgtggaatta tcctcactgg cacttttttc catgatcctt cttcaggatc ctggagcggc
    22201 acggctttac attttctccc agcttgttgg agcaggattc ctgctggcgg gaacagccct
    22261 cctgagcgca gaaacgggaa ccatttccat aggcccggtt ccgggaaatc tcctgtggct
    22321 cttccttacc ggtcttggct taaaggccgc ctttcctgtg ctgcatttct ggcttcccga
    22381 ggtccacagc agggcacctt cgccagccag cgcgctcctc tccggctttg ccgtgaagat
    22441 aggcatcttc ggcctggcaa agcttgcctc gcccgaaaca ggtacattct ttctggctac
    22501 cggttcagca atggcccttt acggtgttgg ccaggccctg ctgcagcatg acgccaagag
    22561 gctgctcgcc taccatacca tcagccagtt gggctacatt atcaccgctt tcggcgcggg
    22621 gaccgctctc gggattgcag ccgggttttt ccatgccgtt gcacatggcc tcttcaaggg
    22681 tctgctcttt ctctcggttg gaaccctgga aaaaatctac gggacaaggg atctgggtca
    22741 cctgggaggg gccgccaggg aaatgcccgt cacctttatg ctttttctgg tgggcgcatt
    22801 gagtatatcc ggctgcccgg ggttcagcgg cttcgcgagc aaggccatga tcaaggccgc
    22861 tctctacgag gcgcccaacc ctgcatggta ctggatactt caggcggcag gggtcgggac
    22921 ggtaatatca ttctgcaagg taggtttttt tgcctttgta agggaaccgg tgcaacggcc
    22981 agaaccaatg ggggagcaga ttccgggagg aagaaaggac tccttcagga acctgggcat
    23041 gctcttgccc gcggccgcaa cggtggctct tggcgttatg cccggctttt tcaccaggct
    23101 cgggggattt tatggcagta acctttttgg gataaaaaac cttttgccag ccatggttac
    23161 cctggccctg gggctcttta tcttccggtt gttccggagg tggttcgagc ccggctcgca
    23221 cgacattccc gatgtcagcg atatttttgc aacagcgtcc gatctttcgg ctcctctata
    23281 tggtttactg ttctccatga actgcggcct tctgcgtttc tacatcgctg ccgtcatcgc
    23341 tgcgggagtt gccctttttc tttttttata aatatcaatg aaaggaatgg tctgaatgca
    23401 cccagaggga aaatcaatga acaggatcat tgttccagcc ggccatgccc gggcttttga
    23461 ggttgaaaag ggaacaactt tcactgtgat cgacatggaa ggcggacaga tagcggacct
    23521 tgtagccttc aacagggaca acctgtcgga aaaattctct tgctcgcata cccgcctcgg
    23581 actgcactct gtcgggttca aggtcggtga ctcgctgcgg acgaacctgc gcaggcccat
    23641 gatggagatt gtgacggaca ccgtaggaac ccacgacatg ctcattcctg catgcgacga
    23701 gcagaggtac aggatagact acggcgtcga ggaacacagg agctgcgttg cgaactttga
    23761 agaagtcctc gcgccatggg gcctggagcg gagggatata cccgatccgc tcaatatatt
    23821 cgagaacgcc gacatcgacg ccgacggcaa tcttgtccat cttcccgtta tttcaaaggc
    23881 cggagaccat ataatcttca ggcctctgat gaacctggtc tgcgccgtat cagcctgccc
    23941 gatggatctc aacatcacgg gtggtaacag gataaccgac attcttgtgg aaatactggg
    24001 accgtgagtc gtgaatcgtg tggtttgtca ttctgacaac caacgggagg aagaatctgg
    24061 gtcaaggaca gatctccgcc ggtgggcaaa gggcgcggaa cgtccgcctg ctggcgggtt
    24121 ccgcttcgtc gcttcgctcc tcaggatgac agcagggtca actgcgtaaa cggtaaacgg
    24181 atctttcagt tccgggcctg gtttttgccg gggcgctact ttgccccggc tttttcatct
    24241 atcggtgagt ggtcttcaat gtagcgggca agggcatctg tgtcggtgaa gccgatatcc
    24301 acagcgcgct tgctgaacgg gatcatcgca tcgtaaccgt cggccccgga ggcaaggtag
    24361 gtacttgtcg ccaggcgata ggtttttgcc ggatcgagag ggactccgcc tattgttatc
    24421 tgatcctttc ctgcttcaac ggaacgggcc ccaatcccgg aaacgtgagg gaaaccgccc
    24481 aatccaggct ttatttgcga tatcgatcca aggatctcag caatatcgct tccactgagg
    24541 tccattacaa ccaggctgtt ctggaatggg agaaccgcca ttacatcccg catccggata
    24601 tcacctgcct ggatggaatc ccttatacca ccggcattta ctacggcgat atccgctccc
    24661 gccacctccc ggatggcatc aaccacgaga cgggtgaggt ttgtctctcc agagcggacc
    24721 ttgactcgtt caccatccaa aagggtccgg gtgcttccta tgacctggtc gatctttctt
    24781 gagccgaccc attcgtaata atccagacgg ttcgtaattt ccggatcttc tcccccggcc
    24841 ggatcgatcg ggatggcctt ccatttccag tccgttatcc gcccatcttc tatctcaagg
    24901 tcaaaacgac ccagatattt gccgaaagcc cctgcctggg caacaagtgt tgatcctata
    24961 agaaccgcgt tatcaaagag cgtgtgggaa tgcccgtcga ttatcacgtc cagacctttg
    25021 atggaggccc tggcaagatc tgcggaacct gtagaacctg gtacaacatt tccccagcca
    25081 aggtgaccga gggcaatgac aacatcggca ttctcccgca gtcttggaac cagatccctt
    25141 gcagtatcaa cggcattgag gaaggatccc ccctccaggt acaggggctc ggatcgggca
    25201 gtactctccg tggtcagacc gaaaaccgca accttgaggt catcgaactc cttgatcaca
    25261 taagggggga aagccaattt tccccatgaa tccacaaaat tcgccgaaat gaacggaaag
    25321 cgggcaaagg actcctggaa acgcaacagg gccaacgggt tatcgaactc gtggttaccc
    25381 aggaccatgg cgtcacaacc catttccctg agaagtgaaa tatcgcagag agcctcctgc
    25441 agatcagact ccggaatccc ggtgttgaca tccccggcgt gcaggaagat tacatccccg
    25501 ccatttgagg caacttcacg acggatctct tccacaagca gcgcaagggc atcgaagcct
    25561 ccctttccct cttcgttcca gatatgaccg tgcgtgtcat tgacgtgaag tatggtcagc
    25621 tgagccgcat ccagcggagc cacaaatacc gccagggcat aaaggccgag aaacaaggcc
    25681 ctggagatcg atcctggatt ccatttcatt cattatccct ccttatatgt aaccactcat
    25741 gccagaacgt tgtttatttc tccggttcgt taaccctgcc catgaagagt atgttgccgg
    25801 tgctgttctc ctggatgatg aacagaaagg gacgatccgc gcggaaaacg acgggagggg
    25861 ccgcgacgga tcttgcccca accacaaccg ccgtggcggc cgctgcctcg gtgccctcct
    25921 cgttgacgtc gacaaaggcc ttgtggagga catctgttat atagagccag tccggtcggc
    25981 catccatacc cgagaaattt gccttgctgc tgctgaaggc gtccaccatg ccgagggagt
    26041 gcagggtctc cacaagagag acagtacccc aggttatcct gaattttggc aggaagagta
    26101 ttactctctc ttctttcagg aacttcttcc aggtttcaaa gttctccagg gtcatctttt
    26161 tttcaaggga cgacagggga gtgtcggtgt ctggaagcaa cactatcatg gagagatccc
    26221 ggccggcata gggcagttct atcatctgga acccggggag ttccgcatat ttgtagttcc
    26281 ctgatctgct catgagaggg gcgttcactt ccttgcccga cgagagggtg aagggggctt
    26341 cttccgtcgc ctcggggtcg aactgatctt cccagttccc cttgaagtat atggcgttca
    26401 ccagcaccat gcgggtcaga ggatcgatgc ttccctgggg gataagttcc ttgatctttt
    26461 cctttgtaat ctcttcgacc cagccgttga tcctctctct ggagggttcc ggctcgcggg
    26521 catagtccag gggggttatt tcgaccccgt aattttcatc aagtagcgac aggtattcct
    26581 tgaggaatgg gtatcctgac tgcggccaga tcgagttcgc tatcgagagt tccacattcc
    26641 cttctttctg gatctcggcc atgcgttccc cgagttcgct gaaggccctg tgagtaagtt
    26701 cctgcccgcc ggtaaaacgc agagcctttt ccatctggga tgccgtctct cctgctgccc
    26761 cgccgtaggt catggcaaga gcggaggata tgctgtaggg ggagaaaaag aggttcccct
    26821 ctttagcccg gatactcgaa aaaatatcaa aggcaaactc cgcattgtcc gatgataccg
    26881 aaagcaggag ctccgacccg aacccggcag acgcggctgc aaggatcaga accaccatga
    26941 taccaatgat ctttgacatg ggtctgtttc tcattgctta cacctccgtt gcctaaattt
    27001 tcctgctgtc gtatgcccag tgaagcaggg cctgccgctg tcttttcctg cattccaggt
    27061 ccccggcaaa acagtatttt ctcagttgcg caatatgtcg tgtcatggcc ttccaccgcg
    27121 atatctgcct gagatcatcg gggcaccttc gccccatgtg atagcggcag taccactgga
    27181 accaccccct gggatcctca tgatgtatcc accccttttg tctccagtag gaaaggggtt
    27241 tcgaggcgtt cactccaaag aggttcaggg ccgggtcatg cttctcggta cacaaacgag
    27301 cgttttcgaa ccaccatgcg gggaactcgt cagcacagtc ggtcatgtat ttccccccga
    27361 atacgcccat ttccagcatt tcagcggggg taaggtctgg cctgaacccg gggtgaaaat
    27421 tctccccctc cggctcggtg atggtatagg agtatccctt ctgcataaga tcgttaacca
    27481 gaacctgcct gttcatgggt tcacttctga tcacagtcac ggcctgtata cgggcatcat
    27541 gattatgagg tggttattgt agggattgaa ggcgttgttg acgaacccga attcggcctg
    27601 cctggatgtg agcacggccc tgacagcagc atttatgcca aggctgctgt tgtcgttgac
    27661 atttgccacc acccagaccg agcctgggtt gtttgcggca gtatcgccta ccctcctgat
    27721 cgcgtagatc ttctcctgct ccttcccttg cgggttagat accagggaat cactcccgat
    27781 atagtccctg actatcgtcc aggccgcagc tgtggtggca ggccaggagc cgttgtcaag
    27841 gtaatacaga gtggccgcgc tcttgaggac ccggaggtca ccgattatca ctgctgacct
    27901 ggccctggct tgcgatctgg aaaaaacaag caggaggctg ccagccagta tcccgattat
    27961 taccagaaca accaatagtt cgacaagggt aaagcctctt ctcttctcac gggtcagggg
    28021 catttggggc atctcccttt ctgtttttca catgggtatt attttaactt cttgtgatta
    28081 acagggcaaa ccgaagactg gttttatttt ttgccacgtt caactatgta atatagaatg
    28141 agtgagaaaa tcaggtttat ttttgctact aacctgaatc tggcaggaga cagatatgaa
    28201 caaggaaaac aacggctcga cctataccag atacaacgcc gaggacctga tcctcaggga
    28261 cgaacttgcc attgacagga ctctcctggc caacgagagg accctccttg cctatcttcg
    28321 atcggcggtg gcacttgtgc ttgccgggtt cacgatgatc catttcgcgg cggattcgca
    28381 ctggttctgg cttgcgggat ttgtctcgat ccccgtgggt gtttttgccg ggatgatcgg
    28441 ggtggtacgg ttccgggtca tgaacaggat tatttcccgg atcaggaagc agaaaagcga
    28501 ggagactgga gagggggatc aggatactgg aaggacaaaa gagccgtaag acgttagacg
    28561 tgggacggag aagcaaggag acaggagaca ggcatctgga ggcagggaaa gctgaaaacc
    28621 aaattcaact gcgtaaacgg tgaacggtgt gaagcagacc aaggagactt tgagactgcc
    28681 accaagatac cgaggggccc tgagttaagt ctttcccgaa aggcaactcg cctgaaatcg
    28741 ttcgtttctc cgcctgctgc ggg
//
LOCUS       DAOTIQ010000015        28366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1901_length_28366_cov_8.423687, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000015 DAOTIQ010000000
VERSION     DAOTIQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 28366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28366
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_28366_cov_8.423687"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="P5541_03535"
     CDS             complement(<1..453)
                     /locus_tag="P5541_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HRV71081.1"
                     /translation="MADFQISGITSGIDWGEIIDQMISNRRTVQVQWLETKETINSRA
                     YLYSELVAGLGGLKSSLDPLVLESTFLGKTAEVTPLSGIEAPLSVKVSPEASIASLDI
                     DVSSVAKNHRVAGDRVEDISSPLGLSGSFSLSVGDSETTVEIAAGNSLS"
     gene            complement(473..1468)
                     /gene="fliM"
                     /locus_tag="P5541_03540"
     CDS             complement(473..1468)
                     /gene="fliM"
                     /locus_tag="P5541_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRV71082.1"
                     /translation="MDVLNQAEIDRLIGALASGEKIEEEDVSEKAVKTYDFRRPNKFN
                     RDQLRAVQMLHESFARHLTSKLSAMSRIMVSAEIATVEQIPYEEFMRSVIAPTTLAIL
                     DVSPYGGKSLLEVSQFLVFALLDRMLGGGGQPLNRSRDCTDLEKVVVDKVLETMVEGL
                     NESWKAIREDFMFSVFGRESNPFFVQIVPKSEMVLLVNIKVQVGTVEGHLNLCFPYHC
                     MEPVIDRFNPQEWFVARDKSYSDGEKEILRKRVSDVRVDLRAELCETSLTLNEISNLC
                     EGDVINLGRPVSGSGILKVGSIPKFSVVFGTSGDRTCARISGVLPPQERDYYSLP"
     gene            complement(1485..1958)
                     /locus_tag="P5541_03545"
     CDS             complement(1485..1958)
                     /locus_tag="P5541_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRV71083.1"
                     /translation="MKPVVKKVIVMAIVLVSVFFLGFAGGVIGSGMMAGRGENSGKVD
                     KPRSYPIFDLGEFKLSLAGGKYTDSRMVSFELVLELQNQKFLEKIGGDDYWRALFRNE
                     IISESMSQGIDSFQSPEGFLRLSEAFTRRINAVTPELSGGELPVRRILFRSMIIQ"
     gene            2096..3226
                     /locus_tag="P5541_03550"
     CDS             2096..3226
                     /locus_tag="P5541_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71084.1"
                     /translation="MKCLLPGHFRLFFPTLILSLFAAAFPIHANGNPGEIPAVVAVIG
                     GSPGDHCFTSANIARAFFQDRQRGGGATRLVAERFPEPLSEKAALELWKTLTDKEVVA
                     AISTSRGIPATFAARAGGRGPFPLILLCSREIAPEMMGEEGPFVFSLDMNESRLLEAL
                     SQWASRERGNWAILTDHLDASSISRGKSVAEALKSAGCEARILVLAGPADTRLIHTVN
                     KCIDDGITHLLSTLAPCGTARIVSILGKTAQRAPDLFYWGEPHDDLRGMEGLSVFSQE
                     QHPPLKGEKTGIFSGFLEDDSTRPVTIKTALACQWILKASFSVREGLSLTLAEAMEEV
                     GTVESFGKSLRISPSQRRPVSGEVFVLKSQNGRWHEVDNIRF"
     gene            complement(3228..3980)
                     /locus_tag="P5541_03555"
     CDS             complement(3228..3980)
                     /locus_tag="P5541_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRV71085.1"
                     /translation="MLVLVLTSLRFLGLFSSLVMFSGAVLPFTVRALMSLVMALAVAS
                     AMGEIQIETAVFTSAAGLAITSSREVIAGVMLGVLASSPLYAIQSAGRFISQQMGFSM
                     ADIMDPMSDQRVSVIGQLKYILGTWFWFWAGGHLLMTAAVVESMKVLPLGTPLFTLFS
                     IEGARAWIRGIMFLSVRMVLPYFGTLLLAELGLGFIARMVPQMNVFMLGFPVKILLGI
                     FLVSVFAISIIRGLLPGAVMDFLGMFTLFWGR"
     gene            complement(4016..4282)
                     /locus_tag="P5541_03560"
     CDS             complement(4016..4282)
                     /locus_tag="P5541_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HRV71086.1"
                     /translation="MDGSFAALFHQAAWTVLRAALPILVAAMAVGLVIGILQTATSIQ
                     EQTLSFVPKILAVILTALFLGPLVFRPVLRLAVEVFGRLHTYIQ"
     gene            complement(4296..5066)
                     /gene="fliP"
                     /locus_tag="P5541_03565"
     CDS             complement(4296..5066)
                     /gene="fliP"
                     /locus_tag="P5541_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300467.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRV71087.1"
                     /translation="MRKMLFSGFVFLISVAVLLHASPAFSQTSSFPAFPVISIGAADG
                     SDPGQVSMTLQILLLITVLSLAPAIALMATCFTRILVVLGFLRNAMGIQQLPPNQVIV
                     TLALFLTLFIMRPVWERVYNDAYIPLQEGTLSTGQAIDEAMVPVSGFMMKHTRDEELS
                     LMVSLSGSERPQDQAEIPLLVLLPAFMLSELKTAFQMGVVIFIPFLVVDMIVSSILMA
                     MGMIMLPPMLVSLPFKVLLFVLADGWDLVVVSLIRSFN"
     gene            complement(5149..5649)
                     /locus_tag="P5541_03570"
     CDS             complement(5149..5649)
                     /locus_tag="P5541_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRV71088.1"
                     /translation="MSTLNMLTTLVQSFSIGFLDVAKALGLHFSIDKGNAGPGVRASS
                     SCAAALIGVVGEKLSGNVIILSDGGAFIEIVRTMSGGAISEPDTSDPMSMSALGELTN
                     MLCGAALKEVARAGSGRLDITPPQLFSGENLKSVPSEGEGVKYFTIPLMSGSGECFVY
                     IVLGFR"
     gene            5812..7113
                     /locus_tag="P5541_03575"
     CDS             5812..7113
                     /locus_tag="P5541_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71089.1"
                     /translation="MSHIAVNVKTGDLQPGQVLAEDVRDFLGRPLLFKGVVLNYRLIQ
                     ALKFRENIEYVKILVSCEEAPQGVLKPDGIGNNIPAGIHQRVGRFFQKAREIHELQPE
                     TVEELSQDIKPIINEIFDNKPAIMDSLQLISDHDNHTHHHSWMVMILSLSMLRMTEMQ
                     NVLKPDRQDKINAALGALLHDVGKTRIPLKILNKPGKLSPEEWDVMKKHPAFGYSIVK
                     GTPNLLPISKAIVGHHHLLLDGSGYSAEGLPPLDRIPPLVRIVTVADIYEAIVSDRPY
                     HFAALPYHAMQIISQGAGTRFDSRFIDALDQMVAPFPTGSFLLFTQGTMAQVVRVEAT
                     EKDNPIIRIIGSLSKGTHSLVGRICHLYDNLPGMPGERDLVLGAYSPQSLAEKIIEAI
                     KVGNRIENIVGQPSDIHLSCLPEWEEVLAESLEILLQKTRT"
     gene            complement(7136..7498)
                     /locus_tag="P5541_03580"
     CDS             complement(7136..7498)
                     /locus_tag="P5541_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV71090.1"
                     /translation="MAKIMIVDDAAFMRMMLKDILTKGSHEVVAEAEDGKKCLQIFPE
                     AKPDLVTLDITMPELDGMNTLLALKKLDPSIKVVMVSAMGQQESVIQAIKAGARDFVV
                     KPFQPDRVLDAVEKALRS"
     gene            complement(7549..7866)
                     /locus_tag="P5541_03585"
     CDS             complement(7549..7866)
                     /locus_tag="P5541_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71091.1"
                     /translation="MAYRKTGVLLPTAGEMAACTREPSRSRLLRERIEKYYSELRSGL
                     PRPGRDEPDLIRKYGEVMGRLRMEEETVIEMLEAFVSGDAPKVRETSVRIARRQEKTF
                     SAR"
     gene            complement(7866..8891)
                     /locus_tag="P5541_03590"
     CDS             complement(7866..8891)
                     /locus_tag="P5541_03590"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRV71092.1"
                     /translation="MSVAKVVPAPDLLEREIDRPFFESFQARELKRTIDLTSTAAGNS
                     RNGMTIAVVSGKGGVGKSNFALNLALSLGDSYGVRTAIIDADLGLANTDVLLGLETRL
                     DLGHVIRGELSVRDIVIPVAEKVWLVPGGSGMTELSAMTDEDWGKVLPEILSLDDMAD
                     ILVIDAGAGISSHVLECALASDVIVLLTTPEPTALRDAYGMIKNLLHRSGPRGLSGKD
                     LSLVVNMAFSAKEAMLASGRLQDVSRRFLGLELSFLGYIPHDLRIGRSVKAKCPFIRL
                     FPQSVPSHRLGEMAGRLLAGWPGRRDVERRPCQGPMKRLLSSLLKNEPEGSNRIVPDI
                     LGGKEAL"
     gene            complement(8888..10006)
                     /locus_tag="P5541_03595"
     CDS             complement(8888..10006)
                     /locus_tag="P5541_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71093.1"
                     /translation="MRLVREIEFEAKSENEAFCKVKSRLGSDGVILSMQNLRTASIIP
                     FLRKTRIVVRAGILEEEKKLPPRPDPDLEKKRMEVFQALLVHRKNGDNETVSAMAPSI
                     TVLEEKKAENPKETFLELAELIEHEIPRDCVEKLREEYGRLAEQEGSFPSWLEKKADS
                     FCACPEGDSLVSALGGRRVMVVGPTGAGKTTTIAKIAAMAVMDGMDVALFTSDNFRVS
                     AVDQIRTFARVLGIPVEVVNQGSEIPGLLKKYSPETIILMDTAGCGFRDEERLSAISE
                     VCRYFEPDAVHMTISGPSRARDVKAAMDTARGIFPLSSIILTKLDETVCMGASIWLPM
                     KYGLPLSFLTTGQNVPRDLHLAKGSMIASFLFKTGVPA"
     gene            complement(10047..10859)
                     /locus_tag="P5541_03600"
     CDS             complement(10047..10859)
                     /locus_tag="P5541_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRV71094.1"
                     /translation="MAERIYPAGPGYERFKKDLARLTGIDLDIYKYQIHRRVHTLMNN
                     WGHTDYGEFFRAISSDDVKLGNFLNYITINVTEFFRNPARWDSLREDVLPELRRMYRG
                     SLDFLSAGCSTGEEPYSLAMVARIERIPCRIIALDLDEGALRRAEEGRYREKQIAAVP
                     DDLREASFTSEGDGFFRVRPELKEMVTFKKANLLEQELPSGQHLIFCRNVVIYFSAET
                     KIMLYEKFYKSLADGGFLMVGTTEQIFEYSRIGFEQAGPFLYRKPSGRVLSK"
     gene            complement(10905..11582)
                     /gene="fliN"
                     /locus_tag="P5541_03605"
     CDS             complement(10905..11582)
                     /gene="fliN"
                     /locus_tag="P5541_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRV71095.1"
                     /translation="MDEEKEKQEETTSGEGAEKKPEGISEQVEEPAENGKGNSFAGST
                     GINAEEAEALAKGLEWLDSTRKEIPEIPDFEKIEKEGSGKDNSRQSEIDDLIASVIGS
                     SQETPYGEKGEEVQGEARPTEFPPMEQEGRITSGGPGPDSRMGILLDIPVRVTVVLGN
                     SRKTIGEIVSLEPGAIIELDRIVGEPVDVLANGKLIFRGEVVVIDENFGVRVVEMTKP
                     ASKKGGA"
     gene            complement(11634..12401)
                     /locus_tag="P5541_03610"
     CDS             complement(11634..12401)
                     /locus_tag="P5541_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HRV71096.1"
                     /translation="MFRGVYNSAAAMLVQQHAVDVAANNLANAGTSGFRKSIVSFKAF
                     PDAGVARQENDGSSRGHREGIGNIPLSVVVSESSPVMSPGEIRETGNHLECSIKGEGF
                     FQVSDGNNTWFTRSGLFTLDSEGQMVTHSGLVLMGDGGPIPVGDAAVVSINEKGVVIS
                     DGEEVGRVPLFSFEAPSRLRQVGSSLLAGTVASGAPVRIDPDLVSLVPGSVEGSNVNV
                     VEEMVRMLAASRAYEAVSKSFEAGSEAGRKMIETFGK"
     gene            complement(12426..12836)
                     /gene="flgC"
                     /locus_tag="P5541_03615"
     CDS             complement(12426..12836)
                     /gene="flgC"
                     /locus_tag="P5541_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRV71097.1"
                     /translation="MKVFRAIDTAGSALTAHRVLLDTIAENLANVNTTRTGEGGPYRR
                     KIPVFSEIMKDASGGSGGVSVMEIAQDDSPPRVVHEPGHPDADEDGCVAYPNVSVVRE
                     MADMVAASRAYEANLEVVESARAMWDAATELLRG"
     gene            complement(12839..13249)
                     /gene="flgB"
                     /locus_tag="P5541_03620"
     CDS             complement(12839..13249)
                     /gene="flgB"
                     /locus_tag="P5541_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRV71098.1"
                     /translation="MFRDDLWVIQKAGASGLAKRFRAVADNLANINTPGYSRKEVFFE
                     DRLREAMDGKTSLSPARVSATEKRHIDPDHKPDLEGIVLETRAENESCRIDGNSVDPE
                     IEMAKLARTRMAYNSVMKLMAKRAEMIKTSMGGR"
     gene            complement(13286..13972)
                     /locus_tag="P5541_03625"
     CDS             complement(13286..13972)
                     /locus_tag="P5541_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71099.1"
                     /translation="MDGKEANLEEKGSRGKRVFRILGGVLLFGALLSGIVAGLLFGGG
                     LYHLSDSLAPMVFDIPFVGAPVARFLQGLTPAMRGSDRRAIEIEQKEQQIVKMLLDLE
                     REKRSLEKREKELALVKKDSEEKLRVLRSEDEAMVNTDEEEPRFYGMFVESFEEMPPS
                     RAAKIVELLSIREAVELFGFLGPEQRSDILSKMPPEAAARLLRYARQQEEGLPPELVL
                     PGAAEASEGR"
     gene            complement(13995..14339)
                     /locus_tag="P5541_03630"
     CDS             complement(13995..14339)
                     /locus_tag="P5541_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71100.1"
                     /translation="MPDNGCYVLQIFEIVENSRPWMEMPVRRFRDQFRSVPPASGLGS
                     FIENGQSLVDWDLFVCRSFEKARELMLDGGLLSVDWYSCYEENDSLDTDLAVFRSTDN
                     RYLGRIIKTELF"
     gene            complement(14387..15040)
                     /locus_tag="P5541_03635"
     CDS             complement(14387..15040)
                     /locus_tag="P5541_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71101.1"
                     /translation="MMEKCIEKVIASLEWELEEIETGDEIPGISSDPVKKRHCLSLSP
                     ETSKNGMDYLVFLCHASLAERVHPLFSSLLVESGDLLPENVFLETVWPALCLSRIWFA
                     DAMAYDLFPDGGLARTEARLRGIEELFPGGRLDGSVSDLAEMALVFAEGNVLCGLKQE
                     FGGKMEEMVAAFSEADPRQPTLDALLSLNNRLLSVWTPFRVEVAFSKELGMELWKVL"
     gene            15244..16086
                     /locus_tag="P5541_03640"
     CDS             15244..16086
                     /locus_tag="P5541_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HRV71102.1"
                     /translation="MDILAVGSLIATFGLVIGAIIITGSPGAFISLPSMLITIGGSFG
                     AVALSASSEELRNIPYSLKKAFMKQKIHNLPQAREVMVDLSRKARSEGLLALEEMVRD
                     IKDEFIRKSTQLVIDGTAPEVVQSILDTEIDLLERRENSSRRVFDLLGELAPAFGMLG
                     TLIGLIQMLRNLDTPEALGPGMALALLTTLYGSMIANAFAIPVSKKIAVQTSSKIAYM
                     EIVVEGVLSIQAGENPRLVEEKLGVFLPPEERVRSRGKRGTKDKKEKLPRGVRTGEGQ
                     PSRG"
     gene            complement(16068..17930)
                     /locus_tag="P5541_03645"
     CDS             complement(16068..17930)
                     /locus_tag="P5541_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71103.1"
                     /translation="MKKKRIARVVVLVLFSSAVSLSGAFAESLVLEKAPDRRFSEEIL
                     ASAYREALGGRVKVAMDTLEQSLRWDPYLVEYYLLKGYCLYLTGDLPGARDNIELYLE
                     VRKDDPFASGFLRQVNDRKKLVEEGLEFGVSSSLFVDVPSGLWERLRIPLISKPALSL
                     PGRPAITGDILTFCDTGSGGLQIFQRTQARGWVKYFDDIVEGNLVRAIPIDRERIFLV
                     FNDGTVVQKMLAKSGLLGVSESIGTAVAISDACAVNSSLLAMADRISGEILFTEPSTG
                     EVIGKWAPGEAVFEPVSVSSLGPLLAVADRMGKKVRVIDIQKMKELYSFEIPGPVRSI
                     EWISSEKLVALTEEGELFGISIYGKEAELVGETFPEAWFLFRDGGEILVTDTRLFRSS
                     AVSAISPRGIMVASEPGSDQNGLEESSFAVIATMIRPLGFRREQEILFQGVLGGKFLD
                     AVINPLPGGAGNGRLRPLDGKNWDLWSSGSISGASGAYILDAADAPLDEESITRMGFF
                     AVSRGLTINLVLGDRIPPLSLVRLAEITGGRTVISADNTGFLPGPEKWELRLQVDGRI
                     LMIGDVEDGGGLYLLGRSGPMLFEDRFPLWRVFVSSREKEKEPTEVPATQPLDG"
     gene            complement(17965..19923)
                     /locus_tag="P5541_03650"
     CDS             complement(17965..19923)
                     /locus_tag="P5541_03650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRV71104.1"
                     /translation="MIETRDKYLEPFLDEAEENIQRLNECLLTIEQGDSAVVDEVFRV
                     AHTLKGMSATMGFQNMAHLTHKMEDVLEGARKGVLDINSGEGTSTLFECLDTLNMLLG
                     EIREKNDDSGVGTEGIVEKLSGLMVMLPREEDRKTSHEVSVPAGDNGAGLRMVEVKLQ
                     PDCMLKGARAFMVFEEVGPLGRVIQSVPHEKELLEGAFQGDVFYFVMETERDPDEIRM
                     AVMNVGEVLSCNVEIAAEKTATVKDRESEAALPKTDSGGAGTRHGHANDHVTAGQTVR
                     VDVHRLDKLMNLVGELVIGRSRIEMLAREQGIKVFEGPILQLGRISMEIQELVTKLRM
                     LPVRFIFDRFPRIVRDISRELGKKVELQIVGQETELDRSVIDEIGEPMVHLIRNSLDH
                     GVETPAERVSRGKNETGLLRVSAFQDGSSVVIAVDDDGRGIDREAVRRKALEKGLLTE
                     EQAENISERDLSQILFTPGFSTSDSVTDLSGRGVGMDAVRNRVEALGGRITVHSVPGE
                     GTGVRIKLPITLAIVLALLVRIDGRSYAIPLESVEETIRIPVNEVKNVNGSLAAQVRG
                     TVLPLVDSRAFYGLSEKHRDGDHEVVVIRTGFRKTGFVVDEFIGQQEIVIKSFGDTGL
                     PRLKWFSGGTILGDGSVALIIDPSALDQ"
     gene            complement(19964..20353)
                     /locus_tag="P5541_03655"
     CDS             complement(19964..20353)
                     /locus_tag="P5541_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71105.1"
                     /translation="MSFQNLRNCRICGKLYTHFRGPDACDSCKKNLDEVYHRAREVLR
                     DAEPGQEYDNISLAEKLDVEPVMIHILAEEGLFERDGLHLSGGEDRKALAREFEMELR
                     KKEQEKIEKKDSTGGMFMDIRRGKRDQ"
     gene            complement(20442..21176)
                     /locus_tag="P5541_03660"
     CDS             complement(20442..21176)
                     /locus_tag="P5541_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRV71106.1"
                     /translation="MAEGKRRKKRPAEQEKSGNWLTTYGDMVTLILTFFVLLYSFSTL
                     DVIKFQKMIFSFRGAIGVIDGGRTFEEDKAVFSGRTMTDSGQSRKQTPDILKVAQKIQ
                     AIIRDEGLEKEVTVTVDQRGVVVSIAEGLLFATGQYELSRGGESILLILGMILEEIDN
                     ELSIEGHTDSVPLNRGGAVSDNWALSSLRAARIAAFFEGRTGISSRRIRAVGFGSTKP
                     IMPNDSDRHRQMNRRADIVIVAGEGI"
     gene            complement(21213..23351)
                     /gene="flhA"
                     /locus_tag="P5541_03665"
     CDS             complement(21213..23351)
                     /gene="flhA"
                     /locus_tag="P5541_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRV71107.1"
                     /translation="MALSASKSTRLSPIEAMMNYSDMGITILVVVIIAMMVIPLPTGI
                     LDILLALNITFAVVVLLMTFYVKSALEIAAFPSILLIATLFRLALNVSTTRLILLNGF
                     AGKLIGAFGAFVVGGNYVVGGVVFLILVLIQFLVITKGAERVAEVQARFQLDGMPGKQ
                     MAIDADLGNGLIDEPTARVRRRIVQREADFFGAMDGASKFVKGDAIAGLLITTINIIG
                     GISIGTFQQGLSLNQAVQRFSLLTVGDGLVAQIPALILSTATGILVTRSAGESNLGRD
                     IGKSLLAYSRPLLVGSGLLFFLSLVPGFPRIPFIILSMFLGLQGYWVSKEGPSEKEEQ
                     GEQIEAHKQAAPQGPQTQDAPARGASGSFDSAMKLLAVDPLELEVGYALIPLVEASKG
                     GDLLDRIQTLRRQMAMELGVVTPPVRIKDNLQLKPSAYVIKVRGVPEKMAELVPDQYL
                     AIDTGNTKGELVGIPTREPSFGLDAFWIAPEIRNKAENLGYTVVDAPSVLATHLSEVV
                     RANAADLLTRQTVQNMVDIVREGAPAVVEEMLQNLSLGDVQKVLQNLVREKVPIRDLA
                     GIFETLADYGRSVQSFDFLSERAREGLKRVISSTYVSEETGELTVVTLSPEWEKKIKE
                     SVQGNLVEGWRLKMDTAEMQLFIKAAGTSTEAMLRAGLPQIILCHPDVRLYVRKITEK
                     AFPNVAVLGYNEIGGDVKLKTIGVVDGSGA"
     gene            complement(23409..25016)
                     /locus_tag="P5541_03670"
     CDS             complement(23409..25016)
                     /locus_tag="P5541_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRV71108.1"
                     /translation="MRRFLREMAGDEKAFQIEEREGDLFLRVLSGEVSLQVILGELEK
                     RGRCMSLALLEKVVSESEEEWKRIPCPSEYEEEKIDVRLSPDKLSAEITIAPGSVGTR
                     ECLEERSLQLLVSKGVKQELVDPGLLSQAIDERGRWKKVVFGKTPVEGVDARLEVSVE
                     TDRKAPAGHDAEDGDVDHKDLGIIHNVWRDQEIVVKIPMQEGEDGFDVTGKTLRAKRP
                     RDVKIVEGPNTELSEDGMVLRSKEDGHLVRDGFRFRVEPVFEVAGDVDYSTGDLDFAG
                     ILVIHGSIRDGFSVTAGKKMEIGGTVEGARVISEGDINIRSGAMGMGRAFIQSGESIF
                     AGHLEQCSVRAGVDLVFRQAIMHCDVEAGSSIRHTGEGKGHITGGVLKAGTEVECMFL
                     GSEMGTRTVVHVGVSPELLEKRSQLLSGQSELEEKKQMIVKNLSYLARLMKEKGLDEQ
                     HRAMALRLDELLRTVREQLGKIAPLIEDIERVIDSNKKKGNVKVWNVCYPGTVIAIRK
                     ETLIIREALERVRFIYDEGKIRMVSLE"
     gene            complement(25041..25664)
                     /locus_tag="P5541_03675"
     CDS             complement(25041..25664)
                     /locus_tag="P5541_03675"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV71109.1"
                     /translation="MKTPTGIPVAGVRKIEARIREICVRFEDTVPPRRPPEFVEVLKK
                     VEKKEKPGPGPSPVKSGREIPGYARPEGGPGRVAPDILESMILRKSREHGVDRNLIRA
                     VVQMESGGKANVVSSAGAMGLMQLMPSTARMLGVEDAFDPEQNMDGGVRYLQMMLDRF
                     NGDEKLALAAYHSGPGRVEEYGGIPPFPIVNRYVKTVSAIVEKLRRE"
     gene            complement(25700..26146)
                     /locus_tag="P5541_03680"
     CDS             complement(25700..26146)
                     /locus_tag="P5541_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HRV71110.1"
                     /translation="MTTRKKINRLEKLRHIKDRLKEEAKVRVSDQRQKEALLEKQTEV
                     LETRLEKNISLFRERSSGGIITPDELWCLRSDIDSIEEEIRIIGEAMEKTRREMDILQ
                     EDLISKNRETRLTEALLEVHKCERRREIIDSEQKELDDLVCMKFSK"
     gene            complement(26146..27453)
                     /gene="fliI"
                     /locus_tag="P5541_03685"
     CDS             complement(26146..27453)
                     /gene="fliI"
                     /locus_tag="P5541_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRV71111.1"
                     /translation="MGKRLPEVQLVPVNGKVADVIGIVIESRGPDVQMGEICEVRIRN
                     RKTEPLLCEVVGFRGERVLLMPLGDVQKIGPGSDVLSTGRTLGVRVGPELLGRVLDGM
                     GEPLDEKGPVIGTDYYPLHSTPPHPLKRKMIESPLPVGIRAIDGLLTLGQGQRIGIFS
                     GAGVGKSTLLGMMARNTGADVNVIALVGERGREVQDFLRKDLKEEGMKRSVVVVATSD
                     QPPLVRLRAAMTATAISEYFRDQNKRVLLMMDSITRVARAQRDVGLAIGEPPATRGYT
                     PSIFEFLPKLLERAGAGERGSITGIYTVLVEGDDMNEPIADTVRGILDGHIVLSRDLA
                     VRNHFPAIDMLQSVSRVMRDVVPNEQMDVAGRIREIISVSKEAEELLSLGAYRKGNNP
                     RLDFALEKSELINAFIRQTIDGYSSFEETTERLLSMPLTEDYE"
     gene            complement(27494..>28366)
                     /locus_tag="P5541_03690"
     CDS             complement(27494..>28366)
                     /locus_tag="P5541_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRV71112.1"
                     /translation="QRNRIGLIRQSHIPENVRLSDEPYAVPLPDGPLPAGDSDGGGFE
                     GGRQIDPYLMAGARIQDLHKTIESLIERERKHLQEIEELKKKLGEEQARLSREEALLR
                     QRMEEAAETARSEAAEKGMEEGYSEGLAAARGEIIEEVREEFRTRFGSTEDLLSRLLE
                     SLDSERERIVEMNAPLMVKLWQVMLERLLRREVKLDGDTTLRVFMELLPRVNDRTRIR
                     VLVNPGDRDALVERGEEFVEIKRSAGSFELVGDEYIEKGSCLIETNMGVYDATWKSQI
                     EAVGREIDHLMEEA"
BASE COUNT         6379 a         8393 c         6687 g         6907 t
ORIGIN      
        1 ggaaaggctg tttcccgccg ctatctcgac ggtcgtctcc gagtctccca ctgaaaggga
       61 gaaactgccc gaaaggccaa ggggggaaga gatatcctcc accctgtcgc cggcgaccct
      121 gtggttcttc gccaccgagg acacatcaat atccagggag gctatggagg cctccgggga
      181 aaccttgacc gagagagggg cctcgatccc cgacagcggc gttacctcgg ccgttttccc
      241 caggaacgtg gattccagca caagggggtc aaggctgctt ttcagacctc caaggccagc
      301 aaccagttca ctatagagat aggccctcga gttgatggtt tcctttgttt ccagccactg
      361 tacctgtacg gtccgcctgt tggagatcat ctggtcgatt atttcgcccc agtcgatgcc
      421 ggatgtgata ccgcttatct ggaagtccgc catatcttcc tccatcccct ttttaaggca
      481 ggctgtagta atctctctct tgcggaggca ggactccgga gatccttgcg caggtcctgt
      541 cccccgatgt tccgaaaaca acactgaatt tcggtattga tcccacctta aggatgccgc
      601 tccccgaaac gggcctcccg aggttgatca catccccttc gcaaaggttc gatatctcat
      661 tcagtgtgag tgatgtttcg caaagctcgg ccctcaggtc gaccctgacg tcactgaccc
      721 tttttcgaag tatttccttc tctccgtcag aataactctt gtccctggcc acaaaccact
      781 cctggggatt gaagcggtca attaccggtt ccatgcaatg gtagggaaag cagaggttaa
      841 gatgtccctc aacggtaccg acctgtactt tgatgttgac gagaaggacc atctcgcttt
      901 tcggcactat ctgcacgaaa aagggattgc tttccctgcc gaaaacagag aacatgaaat
      961 cttccctgat ggctttccag ctttcgttga ggccctccac cattgtctcc agaaccttgt
     1021 ccaccactac tttttcaagg tcggtacagt ctctggaacg gttcagcggc tggccacctc
     1081 ccccgagcat cctgtcaagg agggcaaaga cgagaaactg gctgacctcc agcaaagatt
     1141 tacccccgta agggcttaca tccagaatcg caagggtcgt aggggctatc actgaacgca
     1201 tgaattcttc gtagggaatc tgttccacgg ttgcaatctc cgcggatacc atgatccttg
     1261 acatggccga caatttgctc gtaaggtgtc gggcaaaact ctcgtgaagc atctgaacgg
     1321 cccttaactg gtccctgtta aacttgttgg gtctgcggaa gtcgtatgtc ttgacagcct
     1381 tctcgctgac atcttcttcc tctatcttct cacctgaggc cagggcccct attagtctgt
     1441 ctatctcggc ctggttcagt acgtccattt ctccaccttc ccgcctactg gattatcatt
     1501 gacctgaaga ggatcctcct gacgggaagt tcccctccgg agagttcggg cgtgacggca
     1561 tttatgcgcc tggtgaaggc ttccgagagt ctgaggaatc cctcggggct ctgaaaggaa
     1621 tcgattccct ggctcatgga ctctgagata atctcgttcc ggaagagggc tctccagtag
     1681 tcgtcacctc cgatcttttc gaggaacttc tggttctgga gttcaagaac aagttcaaaa
     1741 ctaaccatcc tggaatctgt gtatttgccc ccggccagag agagtttgaa ctccccgagg
     1801 tcaaatatcg ggtaggatct gggtttgtca actttcccgg aattctctcc cctgccggcc
     1861 atcatcccgc ttccgatcac cccgcctgcg aaaccaagga aaaaaacgga taccagcaca
     1921 attgccatta caattacctt tttgacaacc ggtttcatac agcggaccac cacctggata
     1981 aaaatggacg acgaacctat acttctatat aagaatcggc tgttttcctc tcatacatta
     2041 ccaggcagaa aaagggagaa gccatacagg aacctcttca agggagagtt gccagatgaa
     2101 atgtctactg cccggccatt tcaggttgtt ttttccgacc ctgatcctgt cccttttcgc
     2161 cgctgctttc ccaatacatg cgaacgggaa tccgggggag attcccgcag ttgtggctgt
     2221 cataggaggt agccccggcg atcactgctt cacctccgcg aatattgcaa gagccttttt
     2281 tcaggacagg caaagagggg gaggtgcaac ccggctggtg gcagagcgct ttccggaacc
     2341 cctctccgag aaggcggccc ttgaactctg gaagactctt acagacaaag aggtcgttgc
     2401 ggcgatcagc acatccagag ggataccggc gacatttgcc gcaagggcag gaggcagggg
     2461 ccccttcccc ctgatccttc tctgttccag ggaaatagca ccggaaatga tgggcgagga
     2521 ggggcctttt gttttctccc tggacatgaa cgaatcccgg cttctggaag ccctttcgca
     2581 atgggcctcg agggaaaggg gaaactgggc catattgacg gaccacctcg atgcttcttc
     2641 aatctcccgg ggcaaaagcg tcgcagaagc tctcaaatct gccggttgtg aggccaggat
     2701 cctggttctg gcaggacctg ccgatacccg gctcattcat accgtaaata aatgcatcga
     2761 tgacggcatt actcacctgc tctcgaccct ggccccgtgt gggactgcca ggatcgtatc
     2821 catactcggt aagaccgctc aaagagctcc ggaccttttc tactgggggg agcctcacga
     2881 tgacctccgg gggatggagg gactgtcggt tttcagccag gagcaacatc cccccctgaa
     2941 aggcgaaaaa acagggattt tttcgggatt ccttgaggat gactccacga gacccgtaac
     3001 gataaaaact gccctggcct gccagtggat actaaaggcc tctttctccg tcagggaagg
     3061 tctctcgctg actcttgccg aggcaatgga ggaagtcgga acagtcgagt ctttcggcaa
     3121 atccctgagg atctctccct ctcagaggcg ccccgtctcg ggcgaagtct ttgtgctgaa
     3181 atctcaaaac ggcagatggc acgaggtgga caatatcagg ttttgagtca ccgtccccaa
     3241 aaaagggtga acatccccag gaaatccatc acggcccccg gcagaagacc ccggatgatc
     3301 gaaatggcaa agacactgac aaggaatatt cccaaaagta tcttcacagg gaacccgagc
     3361 atgaacacgt tcatctgcgg aaccattctg gcgataaatc caagccccag ttcggccagg
     3421 agaagggtcc cgaaatatgg tagcaccatc ctgacggaca ggaacattat cccccttatc
     3481 caggcccggg ctccttcgat ggaaaagagc gtgaataatg gcgttcccag gggcaggacc
     3541 ttcatgctct ctaccacggc ggctgtcatg agcagatgcc ctcctgccca gaaccagaac
     3601 cacgtaccca ggatatattt cagttgacca atgaccgaga ccctctggtc ggacatggga
     3661 tccatgatgt ctgccattga aaaccccatc tgctggctga tgaaacgacc ggcgctctgg
     3721 atagcgtaaa gaggagacga ggcaaggaca ccaagcatca cgcccgcaat gacctccctt
     3781 gaagaagtta tggcgagtcc tgcagcactg gtaaaaaccg ctgtttcgat ctggatctct
     3841 cccatcgccg aagccacagc aagggccatt accagcgaca tcagggccct gacggtaaag
     3901 ggaaggaccg caccggagaa catgacaagg gatgaaaaca ggcccaggaa acgcagcgac
     3961 gtcagaacaa ggaccaacaa cagcgtgaga acatccccct gcgacacgac agccattact
     4021 gaatgtaagt gtgaagccgc ccaaatactt cgacggcaag gcgaagcacc ggccgaaaga
     4081 caaggggccc cagaaacagc gccgtaagga tcaccgcaag gatctttggg acaaaggaga
     4141 gggtctgttc ctgtatggag gtggcagtct gcagaatacc tatcaccagg cccacggcca
     4201 tggcggcaac aagtatcggg agggctgccc tcagtacggt ccaggcagcc tgatggaaaa
     4261 gagcggcaaa agacccatcc atatatttgt ccccctcaat taaaacttct tatcaggctc
     4321 accacgacaa gatcccagcc atctgcaagg acgaagagca gcaccttgaa cggaagcgac
     4381 acaagcattg gcggaagcat tatcattccc atggccatga gaatgctcga aactatcatg
     4441 tcaaccacaa ggaaagggat aaaaatgacc acacccatct ggaatgccgt cttgagttca
     4501 ctgagcataa atgcgggaag aagaaccaga aggggtatct ctgcctgatc ctgaggtctt
     4561 tccgaacccg agagagagac catgagagac agctcttcgt ccctggtatg cttcatcatg
     4621 aacccactga cagggaccat tgcttcatcg atcgcctgcc cggtgctcag ggtcccctcc
     4681 tggagcggta tgtatgcatc attatagacc ctctcccaga cagggcgcat aataaaaagc
     4741 gtaagaaaaa gagccagcgt tacgataacc tggttgggcg ggagttgctg gattcccatt
     4801 gcgttcctga ggaaaccaag cacaacaagt atcctggtaa aacaggtggc catcagggca
     4861 atggccggtg ccaggctaag cacggtgatc aacagcagta tctggagcgt catcgagacc
     4921 tgtccggggt ctgacccgtc ggccgcgcca atgctgataa ccgggaatgc cggaaaggaa
     4981 gatgtctggc tgaaggccgg ggatgcgtga aggagaactg ccaccgaaat aaggaaaaca
     5041 aaccccgaaa aaagcatttt tctcatagcg atccctcttt gtgttcaaat acgtttatta
     5101 gcctgttact tataatataa ctaataaacg tatttgtaca taccgggatc agcggaaacc
     5161 tagaacaata tagacaaaac attcccccga cccactcatc aggggaatgg tgaagtactt
     5221 gacaccctct ccttcggagg gcacggattt cagattttcc ccggaaaaaa gctgcggggg
     5281 ggttatgtcg aggcgaccgg atccggccct tgcgacctct ttaagtgcag ccccacagag
     5341 catgttggtg agttcgccca gagcactcat ggacatgggg tccgatgtgt caggctccga
     5401 aatggcccct cccgacatgg tcctgacaat ttcaatgaag gctcccccat ctgagaggat
     5461 gatcacattc ccgctcagct tctctcccac caccccgata agggccgcgg cacagctgga
     5521 cgaggcacgg acacccgggc cggcatttcc tttatctatg ctgaaatgaa gccccagggc
     5581 tttcgcaacg tcgagaaaac cgatactgaa agattgaacc agggttgtaa gcatattcag
     5641 ggttgacaat aaattcactc tccattgttt agggggctgt tttctgttat tacttacttg
     5701 tgtaactcga cttgacatcc aattatttaa taaaaatttc agccagatag tttatactga
     5761 ttacggatga aaattatcct taattttcag gtacgtcgga ggagcgattt tttgtctcat
     5821 atagctgtca acgttaaaac cggagatctc cagcctggac aggttctcgc cgaggatgta
     5881 agggattttc tcggcagacc cctccttttc aagggagtag tcctcaacta caggctgatc
     5941 caggccctga aattcaggga aaatatcgaa tacgtcaaga tactggtttc ctgcgaggag
     6001 gccccgcagg gggtcctgaa gcctgacggg ataggcaaca atattcccgc gggcattcac
     6061 cagagagtgg gccgcttttt tcagaaagcc cgggagatac atgaacttca gcccgaaacg
     6121 gtggaagaac tgagccagga catcaaaccc ataatcaacg aaatcttcga taacaaaccc
     6181 gccatcatgg acagcctgca gctcatttcc gaccacgaca atcataccca ccaccattcc
     6241 tggatggtca tgatactctc cctgtccatg ttgaggatga cggagatgca gaacgtactg
     6301 aaacccgaca ggcaggacaa gatcaacgcg gccctcggag cactcctcca cgacgtgggc
     6361 aaaacacgga tacccctgaa gatccttaac aagccgggaa agctctcccc ggaggagtgg
     6421 gatgtaatga agaaacaccc cgccttcggt tattcgatcg tcaagggaac tccaaatctt
     6481 ttgcccattt caaaggctat cgtcggtcac catcatcttc tcctcgacgg cagcggctac
     6541 agcgccgaag ggctaccccc tctggacagg atacccccac ttgtccgcat agtcacagtg
     6601 gctgacatct acgaggccat cgtatccgac aggccgtacc atttcgcggc ccttccctac
     6661 catgccatgc agatcatatc ccagggggca ggaacaaggt tcgacagccg cttcattgat
     6721 gccctcgacc agatggtggc ccctttcccc acaggaagct tcctgctgtt cacccaggga
     6781 accatggcac aggtagtacg ggtcgaagca accgaaaagg acaacccgat aatcaggatc
     6841 atcggttccc tttcaaaagg gacccacagc ctggtcggga ggatttgcca cctctacgac
     6901 aacctgccag ggatgcccgg tgagagagac cttgtgctcg gtgcctattc gccccaaagc
     6961 ctggccgaaa agatcatcga agcaattaag gtcgggaaca ggatcgaaaa catagtcggc
     7021 cagccctctg acattcatct ctcctgcctg cctgagtggg aggaggttct tgctgaatca
     7081 ctggagatac ttcttcagaa aacaaggacc tgataacccg ggtccgtaac aggaatcagg
     7141 atctcaacgc tttttcaaca gcatcgagaa ccctgtctgg ctggaagggt ttcacaacga
     7201 aatctctggc ccccgccttt attgcctgta tcacgctttc ctgctgaccc atggcgctta
     7261 ccataacaac cttgatggag ggatccagtt tcttcagggc cagaagggta ttcataccgt
     7321 caagttcggg catcgtgatg tcaagggtta cgagatcggg tttcgcttca ggaaaaatct
     7381 gcagacattt tttcccatct tccgcttctg cgacgacctc gtgtgagccc ttggtcagga
     7441 tatccttcag catcatccgc atgaaagcgg cgtcatcaac aatcattatt ttcgccacaa
     7501 aaattacctc ccgatccttt cgttgaccgc tacagattct ataccatatc aacgggcaga
     7561 aaaagttttt tcctgcctcc tggctatcct tacagatgtt tcccggacct tcggggcatc
     7621 gccgcttaca aaagcttcca gcatttcgat gacggtttcc tcttccatcc gaagcctgcc
     7681 catcacctcg ccatatttcc tgataagatc cggctcatcc cgacccggac ggggaagacc
     7741 gcttctgagt tcagaatagt atttctcgat cctctccctc agcaaacggc tccttgaggg
     7801 ttccctggtg caagcggcca tctctcccgc cgtcggcaga agaacccctg tcttcctgta
     7861 cgccatcata aagcctcctt ccccccaagg atatcgggca ctattctgtt ggagccttct
     7921 ggctcgtttt tcagcaacga ggaaagcaat cttttcattg gcccctggca tggccttctc
     7981 tccacatccc ggcgtccggg ccatccagca agaagccggc ccgccatttc ccctaaccga
     8041 tgcgaaggaa cactctgcgg gaacaacctg atgaaggggc atttggcctt gacggacctg
     8101 cctatccgga ggtcatgagg tatgtatcca aggaaggaca actccaaccc gagaaatctc
     8161 ctgctgacat cctgcagcct gccagacgcg agcattgcct cctttgccga gaatgccata
     8221 ttaaccacca gggagagatc tttccctgaa agcccgcggg ggccagagcg gtgcaggaga
     8281 ttcttgatca tcccgtaggc atccctgagc gctgtgggtt cgggggtcgt caagagaact
     8341 attacatcgg aggcaagggc acattcgaga acatggctgc tgattccagc cccggcatca
     8401 ataacgagga tatcggccat atcatcaaga gagagtattt cgggcaggac ctttccccaa
     8461 tcttcgtccg tcatggcgga taactccgtc attccggaac cgcccggtac taaccagact
     8521 ttttctgcca cgggtatgac aatatcacgg accgagagtt cgcccctgat gacgtgacca
     8581 aggtcaagcc tggtctcaag acccagcaga acatcggtgt tcgccagccc gagatctgcg
     8641 tcgattattg ccgtcctgac accataactg tctccaagtg acagggccag gttgagcgca
     8701 aaattacttt tgcctacacc acctttcccg cttacgacgg ctatggtcat tccgttcctg
     8761 ctgtttccag ccgccgtcga agtcaggtcg atggtccgct tcagttcacg ggcctggaaa
     8821 gactcgaaga agggacggtc tatctctctt tccagaaggt ccggagcggg aacaaccttt
     8881 gccacactca tgcgggaacc cctgttttga agaggaagga ggcgatcatg cttccttttg
     8941 ccagatgaag atctctggga acattctgcc ccgtcgtcag aaaggacaag gggagaccgt
     9001 atttcatggg aagccagatc gaggctccca tacagaccgt ttcatccagc tttgtgagaa
     9061 tgatactgga cagggggaaa attccacggg cagtgtccat tgccgccttc acatctcttg
     9121 cacgggaggg acccgatatg gtcatgtgga cggcatcggg ctcgaaatac cggcatacct
     9181 cgctgatcgc ggacagtctt tcttcatccc tgaacccgca tccggccgta tccatcagta
     9241 tgatggtttc aggggaatat tttttcagca ggcctggtat ttcggatccc tggttcacca
     9301 cttctacggg tattccgaga accctggcaa atgttctgat ctggtccacg gctgatacac
     9361 gaaaattgtc cgatgtgaag agagccacat ccataccatc cataaccgcc atggctgcga
     9421 tcttggctat ggtggtagtc ttccccgccc ctgtggggcc gacaaccata acccttctgc
     9481 ccccgagagc agagacaaga gaatcacctt caggacaggc acagaaggaa tcggcctttt
     9541 tttcaagcca ggaaggaaaa gatccttcct gctccgcaag gcggccatat tcttcccgaa
     9601 gtttttcgac gcaatcccgc gggatctcgt gctcgatcag ctcggccagt tccagaaagg
     9661 tttctttcgg gttttccgct tttttttctt ccagcaccgt tatgcttggc gccatcgcac
     9721 ttactgtctc attgtctccg ttttttctgt gaacaagaag ggcctggaag acctccatcc
     9781 tctttttttc aagatccgga tctggtctgg gcggaagttt cttctcctcc tcaaggatcc
     9841 cggccctgac aacaatcctc gtcttgcgca aaaagggtat tatcgaagca gttctgaggt
     9901 tctgcatcga aagaataact ccgtctgatc caagcctgga cttgaccttg caaaaggcct
     9961 cgttctcgct ttttgcctcg aattcgatct ctcttacgag tctcaattat cccgcctcct
    10021 ggcccaaaga tcaaactgct gcagcattac tttgacaaca ccctgccgga aggcttacgg
    10081 taaagaaaag gacccgcctg ttcgaaccct atccggctgt attcgaatat ctgctccgtc
    10141 gttcccacca tcaggaaccc tccatcggca agacttttat aaaacttttc gtaaagcatg
    10201 atcttggtct cggcagaaaa atagatcacc acgttcctgc agaatatcag gtgctgaccc
    10261 gacgggagct cctgctccag caggttggcc tttttgaatg taaccatctc tttcagttct
    10321 ggacgtaccc tgaagaatcc gtcaccttcg cttgtgaaac tggcctccct gaggtcatcg
    10381 ggtacagcgg cgatctgttt ttccctgtat ctgccttctt cggccctgcg cagggcaccc
    10441 tcatccaggt caagggcaat tatcctgcag ggtatccttt cgatccgggc caccatggcc
    10501 agggaatagg gctcctctcc cgttgagcac cccgcactga ggaaatccag gctaccgcga
    10561 tacattctgc gaagttcggg aaggacatcc tccctgaggg aatcccaacg ggcgggattt
    10621 ctgaaaaatt cggtaacatt tattgttatg tagtttagaa aattccccaa cttgacatca
    10681 tcgctgctta tagccctgaa gaattccccg tagtcggtgt gaccccagtt gttcatgagt
    10741 gtgtggactc tcctgtggat ctggtacttg taaatatcaa gatcgattcc ggtaagacgt
    10801 gccagatctt tcttgaatct ctcgtaacct ggtcctgcag ggtatattct ctccgccata
    10861 ggtcagcctc ttccctcgat acgggcttta ttaaaaacag aggcttacgc ccctcccttc
    10921 tttgatgcgg gtttcgtcat ctccacgacc cggaccccaa agttttcgtc aattaccaca
    10981 acttctccgc ggaagatcag tttgccattg gccagcacat caacaggctc cccgactatc
    11041 ctgtcgagtt caattatggc tccgggctcg aggctgacta tctctccaat ggttttacgg
    11101 gagttaccaa gaacgacggt gacacgaaca gggatatcaa gaaggatccc catcctgctg
    11161 tcggggccgg gtcccccgga cgtaattctg ccttcctgtt ccatcggggg gaattccgtt
    11221 ggtctggcct ctccttgcac ctcttctcct ttttccccgt aaggagtctc ttgcgaggag
    11281 cctattacac tggctataag gtcatctatt tctgactgtc ttgagttgtc ctttccggag
    11341 ccttcctttt cgatcttctc aaagtccggt atctcgggga tctcttttct ggtcgagtca
    11401 agccactcaa gccccttggc cagagcttcg gcttcctcgg catttattcc cgttgagccg
    11461 gcaaagctat ttcccttgcc attctccgca ggctcttcga cctgttccga tataccctcc
    11521 ggcttttttt cagccccttc tccagaggtc gtctcttcct gtttttcttt ctcttcgtcc
    11581 atgtcttcag gggaaagggt ctcccttcac ctctctttcg gggttcatgt ccgtcacttg
    11641 ccgaaggtct ctatcatctt tcgtcccgct tcggaaccgg cttcgaacga ttttgaaacc
    11701 gcctcgtagg ccctgctggc tgccagcatc cggaccatct cctcgacgac gttaacattg
    11761 gaaccttcta cggaaccagg aacaaggcta accaggtcgg ggtcaattct gacaggggct
    11821 cccgacgcaa cggtcccggc aagaagactc gagccgacct gccgcaatct ggacggagcc
    11881 tcgaaggaaa ataaaggcac ccggcccact tcctctccat cggaaatgac aacccctttt
    11941 tcattgatcg atacaacggc agcatccccg accgggatgg ggcctccatc tcccatcagg
    12001 acaagccctg aatgggttac catctgtcct tcactatcca gtgtaaaaag acctgatctt
    12061 gtgaaccatg tgttattgcc atcgctaacc tgaaaaaacc cttcgccctt tatcgagcat
    12121 tcaaggtggt tccctgtctc cctgatctcc ccgggcgaca tgacggggga actttccgat
    12181 acaacaacgc tcagggggat gttgcctata ccctccctgt gtcccctgga ggacccgtcg
    12241 ttttcctgcc ttgccacacc ggcatcggga aaagccttga aggagactat gcttttgcgg
    12301 aaacccgagg ttcccgcatt ggcaagatta ttggcggcca cgtcaacagc gtgctgctgc
    12361 acaagcatgg cggcggcgct gttatagacc cctctgaaca taccccttcg ccccctgtct
    12421 ccaatttatc ccctcaaaag ttctgttgcg gcatcccaca tggctctggc cgattcaact
    12481 acttccagat tggcctcata ggccctgctc gcagccacca tgtcggccat ttctcttacc
    12541 accgaaacat ttggataagc aacacaacca tcctcatctg cgtcgggatg accgggttca
    12601 tgcacaacac ggggggggga atcatcctgt gctatctcca ttaccgagac tccaccactt
    12661 ccccccgaag catctttcat tatctccgag aatacgggta ttttccttct gtagggtcca
    12721 ccctctccag tacgggtagt gttgacattg gccaggttct cggcaatggt gtccaggagg
    12781 accctgtgcg cggtgagagc acttcctgca gtatctatag ccctgaatac tttcatcgct
    12841 atctgcctcc catcgaggtc tttatcattt cggccctttt tgccatgagt ttcattaccg
    12901 agttataggc catccttgtc ctggccagtt ttgccatctc tatctccgga tctacgctat
    12961 tgccgtcaat ccggcaggac tcattctctg cccgggtttc aagtacgatt ccctccaggt
    13021 cgggcttatg atcagggtcg atgtgtcttt tttccgtcgc agagaccctt gccggagaaa
    13081 gagaagtttt cccatccata gcctccctga gcctgtcctc aaaaaagacc tctttcctgg
    13141 aataaccggg ggtgtttata ttcgcgagat tatccgccac agccctgaaa cgtttcgcaa
    13201 gtcccgaggc tcccgctttc tgtattaccc agagatcgtc cctgaacatc ttgtccacct
    13261 tcctgtctgg ttcttcctgt ttatgttatc ggccttcaga agcctctgct gcaccgggaa
    13321 gcaccaattc gggggggaga ccctcttcct gctgccttgc atatctgagg agccttgccg
    13381 cggcctcggg gggcatcttc gagaggatgt cgctcctctg ctcgggaccg agaaaaccaa
    13441 acagctcgac cgcctccctt atcgaaagta gctccactat ctttgccgct ctcgagggag
    13501 gcatttcctc gaagctttca acaaacatcc cgtaaaaacg gggctcttcc tcatccgtgt
    13561 tgaccatggc ttcatcttcg gaccggagaa cccgcaattt ctcctcacta tcctttttta
    13621 caagggccag ttccttctct cttttttcga gagatctctt ctccctttcg agatcgagga
    13681 gcattttcac gatctgctgt tccttctgct cgatctctat ggcacgtctg tctgatcctc
    13741 tcatagccgg cgtgagcccc tgcagaaatc gtgcgaccgg agctccgaca aagggtatgt
    13801 caaagaccat gggtgcaagc gaatcggaaa gatggtatag cccgcccccg aaaagaaggc
    13861 ccgcgacaat accagaaaga agagccccga aaaggagaac gccccccaga atccggaaaa
    13921 ccctttttcc tctggaaccc ttctcttcca gattggcttc ttttccgtcc attatccctg
    13981 cctccggtat aggatcagaa cagttccgtc tttatgatcc tgcctaaata cctgttgtca
    14041 gtgctcctga agaccgccag gtccgtatcg agactgtcgt tctcctcgta acagctatac
    14101 cagtcaacac tgagcagccc cccgtccagc atcagttccc tggctttttc gaaagagcgg
    14161 catacaaaaa gatcccagtc caccagggat tggccatttt ctatgaagga acccagtccc
    14221 gatgcaggcg gcacgcttcg gaactggtct cgaaatcttc tgacgggcat ctccatccag
    14281 ggtcggctgt tctcaacaat ttcgaaaatc tgaagaacgt aacatccatt gtcgggcaaa
    14341 gagacatcac ccccgaagga ttctttcagg aaagggtcga ttttttctaa aggaccttcc
    14401 agagttccat cccaagttct ttactgaagg caacttcaac acgaaaaggg gtccataccg
    14461 agaggagcct gttgttcaaa gacaatagtg catccagcgt aggttggcgg ggatccgctt
    14521 cggaaaaggc cgcgaccatt tcctccatct tgcccccgaa ttcctgtttg agaccgcaaa
    14581 ggacattgcc ctccgcaaaa acaagggcca tttccgcaag atctgagact gagccatcca
    14641 gacggcctcc agggaaaagt tcttctattc ccctcaacct ggcttctgtc ctcgccaatc
    14701 caccgtcagg aaagaggtcg taggccatgg catcggcaaa ccagatcctg gaaaggcaga
    14761 gagcaggcca gaccgtctcc agaaaaacat tttcgggaag aaggtctccg ctttccacca
    14821 gaagggatga aaaaaggggg tgaaccctct ctgccaggct tgcatgacaa agaaatacga
    14881 gatagtccat cccatttttg gaagtttcgg gggacaggga aaggcagtgt ctttttttta
    14941 ccggatcact tgagatacct ggtatctcat ctccggtttc gatttcttcc agttcccatt
    15001 ccagggacgc aatgactttt tctatgcatt tttccatcaa tctggtacac cttctttctt
    15061 ctgttcacat gacagggagc gacaccccct catatgagtt tcggcatgaa agaccttgaa
    15121 ggaaaggggt cgaaaggtgg atcttcgggt tttttagaaa acgtccggca atcatacttg
    15181 agattgggca gttcccgggg aacccctccg ggaaaacgct accataaccg ggaggaatat
    15241 attttggaca tccttgccgt cggaagtctc atcgccacct ttggcctcgt gatcggcgca
    15301 ataatcatta ccggttcccc gggtgctttc atcagtctgc cctcgatgct gatcacaatc
    15361 ggggggtcct tcggggccgt tgccctttct gcttccagcg aggaactgag aaatattccc
    15421 tattctctga aaaaagcctt catgaagcag aaaattcaca acctgcccca ggcccgggag
    15481 gtgatggttg acctttccag aaaagcaagg tccgaaggcc ttcttgccct ggaagaaatg
    15541 gtcagggaca tcaaggacga gttcatcaga aaatcgactc agctggtcat cgacggcaca
    15601 gctcccgaag tggtccagtc gatccttgat accgagatcg acctgctgga gaggcgagaa
    15661 aactcctcca ggagggtctt tgatcttctc ggggaattgg ccccggcttt tggaatgctg
    15721 ggaacactta taggtcttat tcagatgcta aggaacctgg atacaccaga ggctctcggt
    15781 ccgggaatgg cactggctct tctgaccact ctctatggct cgatgatcgc caacgcattt
    15841 gccataccgg tctccaaaaa gatcgctgtc cagacatcca gcaagatcgc atacatggaa
    15901 atagtcgtgg aaggtgttct ttcgattcag gcaggagaga acccacgcct cgtcgaggaa
    15961 aaactgggag tttttcttcc tccagaggag agagtcaggt caagaggaaa aaggggcaca
    16021 aaggacaaaa aagagaagct gcccaggggg gtcaggaccg gagagggtca gccgtccagg
    16081 ggctgagtcg caggaacttc tgtcggttct ttctcttttt ccctggatga aacaaaaact
    16141 ctccacaaag ggaatcgatc ttcgaaaagc attggtccag accttcccaa caggtaaagg
    16201 cctcctccgt cctcgacatc tcctatcatg aggatccttc cgtcgacctg aagacgaagt
    16261 tcccattttt cgggtccagg gagaaaaccc gtattgtcag cagagatgac tgtccttccc
    16321 ccggtgatct ctgccagacg gacaagcgac aggggcggga tcctgtcccc caggaccagg
    16381 ttgatcgtta gtccccttga gacagcaaaa aaccccattc tagtaatgga ttcttcatca
    16441 agaggggcat cggcggcatc aaggatgtag gccccggagg cacccgaaat gctcccggaa
    16501 ctccagagat cccagttttt cccatcaagg gggcgaagtc ttccgtttcc cgcacccccg
    16561 gggagagggt taatgacagc atcaaggaac ttgcccccaa ggactccctg gaaaaggatc
    16621 tcctgttccc tgcggaatcc aaggggcctg atcatggtag caattacagc gaaagagctc
    16681 tcttccagtc cgttctgatc gctaccgggc tcactcgcaa ccataattcc ccgcggagat
    16741 atcgccgaaa cagctgaaga cctgaaaaga cgcgtgtcgg taaccagtat ttctcctcca
    16801 tccctgaaga ggaaccacgc ctccgggaaa gtctccccca caagttcagc ctctttcccg
    16861 tatatagata ttccgaaaag ttcaccttcc tcggtcaggg caaccagctt ttcggaactt
    16921 atccattcga tggacctgac aggccctggt atttcaaagg agtacaattc tttcattttt
    16981 tgaatatcga tcacacggac tttctttccc atgcgatctg ccacagccag aaggggtcca
    17041 agagaggaga ccgaaacagg ctcaaagaca gcctcgccgg gggcccactt cccgatgacc
    17101 tctcccgtag atggttcggt aaaaaggatc tcgccggaga tccggtcagc catggcaagg
    17161 agagatgagt tgacagcaca ggcgtctgat atcgcaacgg ctgttccgat gctctctgaa
    17221 actccaagaa gtccgctttt cgcgagcatt ttctgcacca ctgtaccgtc attgaaaaca
    17281 agaaagatcc tttccctgtc aatgggtatt gccctgacca ggttcccttc aacaatgtca
    17341 tcaaaatatt taacccaacc ccttgcctgg gtcctctgaa agatctgcag acctccgctg
    17401 cccgtgtcgc agaaggtaag gatgtcaccc gtaatggcgg gcctcccggg gagggataag
    17461 gccggtttcg aaatcagggg tattcgaagc ctttcccaaa ggccggaggg tacgtcgaca
    17521 aagagagagg aactcacccc aaattcgaga ccttcctcta caagtttttt tctgtcatta
    17581 acctgtctga ggaaaccact cgcgaagggg tcgtcttttc tgacctccag gtaaagttcg
    17641 atgttgtccc tggcccccgg aagatcacct gtcagataca ggcagtaacc cttgagaagg
    17701 tagtattcga ccaggtaagg gtcccacctg agactctgct cgagggtgtc cattgccact
    17761 ttcacacgac cccccagagc ctccctgtag gcagaggcaa ggatctcctc gctgaaacgc
    17821 ctgtcgggtg ctttctccag caccagcgat tcggcgaagg ctcctgaaag agagacagca
    17881 gaagaaaaga gaacaaggac gacaaccctg gcgatcctct tttttttcat ctccggactc
    17941 cggaattgtt catatgcccc cagcctactg gtccagggcg gaaggatcta ttatcagagc
    18001 tacactgcca tcaccaagta tcgtacctcc ggaaaaccac ttcagtctcg gcaatcccgt
    18061 atctccgaaa cttttaataa ctatctcctg ctgcccgatg aattcatcga cgacaaaacc
    18121 cgtttttctg aaaccggtac ggatcaccac tacctcgtgg tcaccatctc tatgtttttc
    18181 cgagaggcca tagaaagctc tggagtcaac cagggggagc actgttcccc tgacctgtgc
    18241 cgcaaggctt ccgttcacat ttttgacctc gttaaccggg atcctgatcg tctcctcaac
    18301 actttccagg ggtattgcgt aggatcttcc gtctatccgc accaggagtg ccagcacaat
    18361 ggccagagtt atcggaagtt tgatcctgac tcctgttccc tctcccggaa ccgaatggac
    18421 agtgatcctt ccaccaagag cctcaaccct gttcctgacg gcatccattc cgacacctct
    18481 ccccgagaga tccgttacgg aatccgaagt actgaaaccc ggagtgaaaa ggatctgact
    18541 gagatccctt tctgaaatgt tctccgcctg ctcctccgtc aaaagccctt tttcaagggc
    18601 ctttcttctt accgcttccc tgtctatgcc tctgccatca tcatcgacag caataacgac
    18661 actgcttccg tcctggaagg cgctaacccg caacaggccg gtctcgttct taccccggga
    18721 gacccgttca gccggggttt ctaccccgtg atccaggctg tttcttatga gatggaccat
    18781 cggctcacct atctcgtcaa tcaccgacct gtccagttcc gtttcctgac cgactatctg
    18841 cagttctacc tttttgccaa gttcccggga tatatcacgg actatcctcg gaaaacgatc
    18901 aaaaataaac ctcaccggga gcattctcag ttttgtgacc agttcctgta tctccatcga
    18961 gatccttccc agctggagaa tgggtccctc gaagaccttt attccctgtt cccttgcaag
    19021 catctctatc ctggagcgac caatcacgag ttctcccaca aggttcatca gtttatccag
    19081 gcggtgaaca tcgaccctga ctgtctgccc cgcggttacg tgatcgtttg cgtgtccgtg
    19141 ccttgtcccg gctcccccac tgtccgtttt gggcaaggcc gcttcactct cccggtcttt
    19201 caccgtcgcg gtcttttctg ccgctatctc cacgttacat gagagcacct caccgacgtt
    19261 cattacggcc atcctgatct cgtcggggtc tctctctgtc tccatcacaa agtagaacac
    19321 gtcaccctgg aaagcccctt cgagaagttc cttttcgtgg gggacgctct gtattaccct
    19381 tcccagaggg ccaacttcct caaagaccat aaatgccctt gcgcccttta gcatgcagtc
    19441 tggctgaagt tttacctcga ccatgcggag ccccgcacca ttatccccgg caggaactga
    19501 aacctcatga gaggtttttc tgtcttcttc ccgtggcaac atgaccatca gacccgaaag
    19561 tttttccacg ataccttcgg ttccgacccc actgtcgtca tttttctccc tgatctcccc
    19621 cagaagcatg ttaagggtat ccagacactc aaaaagggta gaggtccctt caccggaatt
    19681 gatgtcaagc acaccctttc tggcgccttc cagaacatcc tccatcttgt gcgtcaggtg
    19741 ggccatgttc tggaaaccca tcgtcgccga catccccttc agggtatgag cgaccctgaa
    19801 cacctcgtcc acaactgccg agtctccctg ctctatggtc agaaggcatt cgttcagcct
    19861 ctgtatattt tcttccgctt cgtcaagaaa tggttccaga tacttgtccc tggtctctat
    19921 caaccttgac cccccccact gcccggcagg tcgccgggag atcctactgg tcccttttcc
    19981 ccctacgtat atccatgaac ataccacccg tactgtcttt cttttcgatc ttttcctgtt
    20041 ctttcttccg tagttccatt tcaaattctc tggccagtgc cttcctgtcc tctcctcctg
    20101 aaagatgcag gccatctctc tcgaaaagac cctcctcggc cagaatgtgt atcataaccg
    20161 gctccacatc aagtttctcc gccagggaaa tgttgtcgta ctcctggcct ggttcggcat
    20221 cgcgcagtac ctcccttgcc ctgtggtaaa cctcgtcaag attcttcttg cacgagtcac
    20281 aggcatcggg gcccctaaaa tgggtataca actttccgca aattcgacag ttcctcaagt
    20341 tctgaaaact cacgtccagg cctcccggaa aaaaatttca cctgctaaca attataggag
    20401 aagtcgcccg agaaaacttt ccgacaggcc agtagcccct atcaaatacc ttctccggct
    20461 actatcacaa tgtcagccct tctgttcatc tggcgatgac ggtcgctgtc atttggcata
    20521 atgggtttcg tggaaccgaa acccaccgcc cgtatgcgcc gggaagagat ccctgtcctg
    20581 ccttcaaaaa aggcggctat ccgtgcagcc ctgagggagg aaagggccca gttgtcgctt
    20641 acagcacctc cacggttgag aggtacactg tcagtatgcc cctctatcga cagttcgttg
    20701 tcgatctctt ccagaatcat ccccaggatc aaaaggatgc tttcacctcc cctggaaagt
    20761 tcatactgac ccgtggcaaa caaaagaccc tcggctatgg aaaccacaac acctctctgg
    20821 tcaactgtca ccgtaacctc tttttccagc ccttcgtcac ggattatggc ctgtatcttc
    20881 tgagcgacct tcaatatgtc gggagtctgc ttcctggact ggcctgagtc cgtcatggtc
    20941 cttccggaga aaacagcctt gtcctcctca aaggtcctgc ccccgtcgat gaccccgatc
    21001 gcgccccgga aggagaagat catcttctgg aacttgatga catcaagtgt cgaaaaggag
    21061 tacagaagca caaaaaaggt cagtataagg gtgaccatgt ccccataggt ggtaagccag
    21121 ttcccgcttt tttcctgttc tgcagggcgt ttttttcttc tcttgccttc agccatctgg
    21181 aaagatcctt ccctgggcac tgtccaacat ttttaggctc ctgacccatc caccacgccg
    21241 atggtcttga gttttacgtc ccctcctatt tcgttgtaac caagcacggc aacgttgggg
    21301 aatgcctttt ccgtaatttt gcgaacgtaa agcctgacat cgggatggca aagaatgatc
    21361 tgcggaagtc ctgccctcag cattgcctcc gtcgatgttc ctgctgcttt tatgaaaagc
    21421 tgcatttccg ccgtgtccat tttcaatctc cagccttcga ccagattgcc ctggacagat
    21481 tccttgatct ttttctccca ttccggcgaa agggtcacaa cggtgagttc ccccgtttct
    21541 tcagaaacgt aagttgaaga tatgaccctt ttcagaccct ctctggccct ttccgacagg
    21601 aagtcgaagg actggacaga ccgcccgtaa tcagccagag tttcgaaaat tccggccaga
    21661 tccctgatgg gtaccttctc tctgaccagg ttctgcaaga ccttctgcac atccccaagg
    21721 ctgagattct ggagcatttc ctcgactaca gcgggggcac cctccctgac gatatccacc
    21781 atgttctgaa ccgtctgtcg ggtaagaagg tcagcggcgt tggccctgac cacctccgaa
    21841 agatgggtag cgagaactga gggagcatcc acaacggtgt aacccaggtt ttcggcttta
    21901 tttctgatct ccggagcgat ccagaaggca tcaagtccga aggacggttc cctggtgggg
    21961 atccctacca gttcaccctt tgtgtttccc gtgtctatgg ccagatactg gtccggcaca
    22021 agttcggcca tcttttccgg gacacccctc acctttatta cgtaagccga cggtttcagc
    22081 tgaaggttgt cctttatcct caccggaggt gtaacaaccc ccagttccat ggccatctgt
    22141 ctcctcaggg tctggatgcg gtccaggaga tccccgcctt ttgaggcttc aacaagggga
    22201 atgagggcat aaccgacttc aagttcgagc gggtccaccg ccagcagttt catggcactg
    22261 tcaaaactgc cggacgcgcc cctggccggg gcgtcctggg tttgtggccc ctgtggagcc
    22321 gcctgcttgt gagcctctat ctgttccccc tgttcctcct tttcggacgg gccttctttc
    22381 gagacccaat acccctgtag cccaaggaac attgaaagaa ttatgaaagg gatcctggga
    22441 aaaccgggaa cgagggaaag aaaaaacagc aatcccgagc caactaacaa aggcctgctg
    22501 taggcaagaa gagatttgcc tatgtcacgc ccaaggttgc tctccccggc tgaccgcgtc
    22561 accaggatcc ccgtcgccgt cgagagtatc agcgcgggta tctgggcgac aagaccgtca
    22621 cctacggtta gcaggctgaa ccgctggacc gcctggttca gactgaggcc ctgctgaaag
    22681 gtgcctatgg atattccgcc aatgatgttt atcgtcgtaa taagcaggcc ggctatggca
    22741 tcacccttga caaatttgga ggcaccgtcc atggctccga agaaatcggc ctcccgctgg
    22801 acgatccttc tcctcaccct tgcggtcggt tcgtctatca ggccattgcc caggtcggca
    22861 tcgatggcca tctgttttcc gggcatgcca tccagctgga acctggcctg gacttcagca
    22921 accctctcgg cccccttggt tatgacgaga aactggataa ggaccaggat caggaacacc
    22981 accccaccca caacgtagtt cccccccact acgaaagcac cgaaagcccc tatgagtttt
    23041 cctgcaaaac cattgaggag tatcagtctt gtggtggaga cgttcagagc caggcggaaa
    23101 agagtcgcta taagcagtat ggaggggaaa gccgctatct ccagggcgct ttttacgtaa
    23161 aaagtcatca gcaggaccac tacggcaaag gttatgttca acgccagcag tatgtccaga
    23221 attcccgtcg gaagaggtat gaccatcatt gcgatgatca ctacgacaag tatggtgatc
    23281 cccatgtcgg agtaattcat cattgcctct atggggctta accgggtgct ctttgaagca
    23341 gaaagggcca tcctctgttt cccccgttcc ttttttcccc gcagtgatca aaaccggttt
    23401 tcaagtccct attccaggga taccatccgt atctttcctt cgtcgtatat gaagcgaacc
    23461 ctctccaggg cctccctgat gatcagggtc tctttcctta tggctattac ggtccccgga
    23521 taacagacgt tccatacctt gacattgccc tttttcttgt tggagtctat aaccctttcg
    23581 atgtcctcta taaggggcgc tatttttccc agttgctccc tgactgttct gagcagttcg
    23641 tcaagcctca gggccatagc tctgtgctgt tcatccagcc ccttttcttt cattagtctg
    23701 gcaaggtatg aaagattctt gactatcatc tgtttttttt cttccagttc actttggccc
    23761 gaaagaagct ggctcctttt ttcgaggagt tccggagata ccccgacatg cacaaccgtt
    23821 ctggttccca tttcgctccc caggaacatg cactccactt ccgtcccggc tttgagcact
    23881 ccccctgtaa tatgaccctt tccttcgcct gtatgcctta tagaggaacc tgcctcgaca
    23941 tcacaatgca tgatggcctg cctgaagacc aggtccactc ccgccctgac agaacactgt
    24001 tccagatgcc cggcaaagat gctctcgccg ctctggatga aagccctgcc catgcccatg
    24061 gccccggaac ggatgttgat atcaccttcc gatatcaccc tggccccttc gacggttccc
    24121 ccgatctcca tttttttgcc tgccgttacc gagaatccgt cccggatgga accgtgaatg
    24181 acaagaattc ccgcaaagtc caggtctcct gtcgaataat ccacatcgcc agcaacctcg
    24241 aatacgggtt cgaccctgaa ccggaaaccg tccctgacaa gatgcccatc ctctttggat
    24301 ctcagaacca tgccgtcttc tgacagttcc gtgttgggcc cctcaacgat ctttacatcc
    24361 ctggggcgtt ttgccctgag ggtcttgccg gtaacatcga aaccgtcctc tccctcctgc
    24421 atggggatct tgaccactat ctcctggtcc ctccagacgt tatggatgat cccgagatct
    24481 ttgtggtcca cgtcgccatc ttcggcatca tggcccgcag gggcctttct gtccgtttct
    24541 acagaaacct ccagcctggc atccacacct tccacagggg tcttgccgaa gactaccttt
    24601 ttccatctac ccctttcatc tatcgcctgc gagagaagcc cggggtcgac gagttcctgt
    24661 tttaccccct tagaaaccag caactgcaaa gatctttctt cgagacactc tctggtcccc
    24721 accgatccgg gtgcaattgt tatctctgcc gaaagtttgt caggggaaag cctgacgtcg
    24781 atcttttcct cttcgtactc tgaagggcag ggtatccttt tccactcttc ttcgctttcg
    24841 gagacaacct tttccagcag ggcaagggac atgcagcgtc cccttttttc aagttccccc
    24901 agaatgacct gcaggctgac ctcgccggag agcaccctca ggaaaagatc tccttcccgt
    24961 tcctctatct ggaatgcctt ctcgtctcct gccatctccc tcaaaaacct ccgcatcctc
    25021 cagaataccc cgggaaggat ctattccctc ctgagttttt ccacaatggc cgatacagtc
    25081 tttacgtatc tgttgacgat cgggaagggg ggtatccccc cgtactcttc aaccctgccg
    25141 ggacccgaat gataagctgc cagggccagt ttttcgtcac cgttgaaacg atccagcatc
    25201 atttgaagat atctgacccc tccgtccata ttctgttcgg gatcaaaggc atcctccaca
    25261 ccgagcatgc gggctgtcga aggcattagc tgcattaacc ccatagcacc tgccgatgag
    25321 acaacgttgg ccttgcctcc tgactccatc tggaccacag cccttataag gttcctgtct
    25381 acaccatgct ctctggattt cctcaggatc attgactcca gtatatccgg cgcaactctg
    25441 cctggtcctc cttcgggacg ggcatagccg gggatttccc tgccactctt gaccggactg
    25501 ggccctggcc ccggtttttc cttcttctcc acttttttca gcacctcgac aaactccgga
    25561 ggccttcggg gcggaaccgt atcttcgaaa cgaacacata tttcccttat tcttgcctct
    25621 attttcctga ctcctgcaac gggtataccc gtgggtgttt tcaaaaaacc tcacctctca
    25681 gaattatcgg accgtttcgt tattttgaaa atttcatgca aacaagatca tccagttcct
    25741 tctgttcact atcaattatt tcccgtcgcc tctcacactt gtgaacctca aggagtgctt
    25801 ctgtaagcct ggtttcccgg tttttgctga tcaggtcttc ctggagaatg tccatttccc
    25861 tgcgggtctt ctccatggcc tcccctatta tgcgtatctc ttcctcgatg gagtcgatat
    25921 cagacctcag gcaccatagc tcgtcgggag tgattattcc ccccgaactc ctctccctga
    25981 aaaggctgat gtttttctca agcctggtct ccagcacctc agtctgcttt tccagaagtg
    26041 cctccttttg cctctgatca ctgaccctga ctttggcctc ttccttgagc ctgtctttaa
    26101 tatgcctcaa tttctcgaga cggttgatct ttttgcgggt cgtcattatt cataatcctc
    26161 cgtcaagggc attgataaaa gcctctccgt ggtctcctcg aacgaggaat atccatctat
    26221 tgtctgcctg ataaaagcgt ttatgagttc cgatttttca agcgcaaaat cgagtctcgg
    26281 gttgttcccc tttctgtagg ctccaaggct gagcagttcc tccgcctctt tcgatacaga
    26341 tatgatctcc cggatccttc ccgctacatc catctgctca ttggggacga catccctcat
    26401 taccctgctt acgctttgaa gcatgtctat ggccgggaaa tggtttctga ccgccagatc
    26461 tctagagaga acgatatggc cgtcaaggat ccccctgacg gtgtctgcta tcggttcgtt
    26521 catgtcgtcc ccctctacaa gcacggtgta gatgcccgtt atgcttcccc tttcaccggc
    26581 accggccctt tcgagaagtt tcgggagaaa ctcgaagatt gacggggtat agccccttgt
    26641 tgccgggggc tcccctattg cgagaccgac atctctctgc gcgcgtgcca cccgggttat
    26701 tgaatccatc atgaggagaa ccctcttgtt ctggtcccgg aaatactccg aaatagccgt
    26761 ggccgtcata gccgccctga gcctcaccag agggggctga tcggaggtgg ccacgactac
    26821 aacggaacgc ttcattccct cttcctttag atccttgcgc agaaaatcct ggacctccct
    26881 gcctctttcc ccgacaaggg caatcacgtt gacgtcggcc ccggtgtttc tggccatcat
    26941 tcccaggaga gtgctcttgc ccacgcctgc ccccgaaaag atgcctattc tctgtccctg
    27001 accaagcgtc agaaggccgt ctatggccct aattccgacg ggaagaggcg attctatcat
    27061 cttccttttc agcgggtgcg gaggagtcga gtggaggggg taatagtccg taccaataac
    27121 ggggcccttt tcatccaggg gctctcccat gccatcgaga acccttccca gaagttccgg
    27181 cccgaccctg accccgaggg ttcttccggt cgacaatacg tcacttccgg ggcctatttt
    27241 ctggacatcc ccaaggggca tcaggaggac cctctcaccc ctgaaaccca cgacttcgca
    27301 aagaaggggt tcagtcttcc ggttccttat cctgacctcg catatttctc ccatttgcac
    27361 atcgggcccg cgggattcga tgacgatccc tatcacatcg gcaactttcc cgttcaccgg
    27421 gacgagttgc acctccggga gccttttccc catcgcctca accaggagtc cgagccttga
    27481 ggatgccacg gtctcaggcc tcctccatca ggtggtcgat ctccctgcca acggcctcga
    27541 tctgggattt ccatgtggca tcgtagaccc ccatgttggt ttcgataaga caactcccct
    27601 tctctatgta ctcgtcgcct acgagttcaa atgatcctgc cgaccttttg atctcgacga
    27661 actcttctcc tctctcgaca agtgcgtccc tgtctccggg atttaccaga accctgatcc
    27721 tggtcctgtc attcactctg ggcaagagtt ccatgaatac cctcagggta gtgtcaccgt
    27781 cgagtttcac ttccctgcgg agaagccttt ccagcatgac ctgccagagt ttgaccatca
    27841 ggggagcatt catctcaacg atcctctccc tctcggaatc cagagattcc agcaagcggg
    27901 acaggagatc ttccgtcgaa ccgaaacggg tacggaattc ttccctgacc tcttcgatta
    27961 tttcccccct cgcagccgca aggccttcgg agtacccttc ttccataccc ttctccgcgg
    28021 cttcagatct ggccgtctcg gcggcttcct ccatcctctg cctgagcagg gcctcctctc
    28081 gagaaagtcg ggcctgttcc tctccaagtt tttttttcag ttcctctatc tcctgaaggt
    28141 gctttctctc tctttcgata agtgactcta tggtcttgtg aaggtcctgt attcttgccc
    28201 ctgccatcag gtagggatct atctgcctgc cgccctcaaa acctcctccg tcggagtcgc
    28261 ctgcaggcaa gggtccatcg ggcagaggaa ccgcataggg ctcatccgac agtcgcacat
    28321 tctccggaat gtgcgactgt cggatgagcc ctatgcggtt cctctg
//
LOCUS       DAOTIQ010000016        27955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1943_length_27955_cov_7.571039, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000016 DAOTIQ010000000
VERSION     DAOTIQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 27955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27955
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_27955_cov_7.571039"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1204)
                     /locus_tag="P5541_03695"
     CDS             complement(2..1204)
                     /locus_tag="P5541_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71113.1"
                     /translation="MKETSRDHSILFDPNLNIIFGITLISVLGVASIAPAFPQITSAM
                     RVPREDAAWLISMFTIPGLLFTPITGALSDRYGRKRILFPCLLLFGLAGGGCFFAPDF
                     RTLLMLRFLQGLGSAPLNSLNVALIGDLFYGKERSAAIGYNTSAIGAGTASYPALGGL
                     LAMIGWNYPFLLAFLALPVGTWVLLGLEEKKLTKTGRIEIGKDLSSLAKSIGSKGTPV
                     ILAASMMSFIVQYGSFLTFLPFFLASTFAATPLMIGLVSSSMSISSAITAPIAGRWFR
                     MERGPLFIASSFFIYGAALFFILHVPGCLGMLVPAVLFGIGQGINIPALLSMLTENSP
                     EKDRAMFLSINSMSLRLGQTVGPIMFGMIFSGFGIRAVFFTGAAMAMVMAVVVLVALE
                     GRGVRVER"
     gene            complement(1234..1749)
                     /locus_tag="P5541_03700"
     CDS             complement(1234..1749)
                     /locus_tag="P5541_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV71114.1"
                     /translation="METKAIDAILSRRSIRKFSGKTVNEATVETLLKAAMSAPSAHNQ
                     QPWEFVVITDREILDSIPSFHPYSKMLLQAPVAIMVCSRTEGLPAEGFWPQDCAAATQ
                     NMLVAANSLGLGSVWLGIFPHQDLVEKSRQVCGIPVEITPFCFVALGYPAEEKGPSKR
                     FDPDRIHENHW"
     gene            complement(1780..2880)
                     /locus_tag="P5541_03705"
     CDS             complement(1780..2880)
                     /locus_tag="P5541_03705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71115.1"
                     /translation="MTQVEMGKVAVEFQRQQRRRKLVTCLVLAGWFATMFLTDVLFGY
                     TFMQKIDGALALAYLACAPATAMLFPFHKRSALAWLAILNLILISSFPVFFVHLGIPV
                     EIILLRQVMTLLFSTLIGPYFIQPKGFAFFLILLYNAACFYYPILHLRELDMMMGYIV
                     VTYSFTLLFSYFFLDILRMNVTHVSSLLTAKKNAEDAALRDDLTGVYNRRYTDNLLRI
                     LIENETDRTLLFIDLDDYQNVCQAHGHLVGDAFLKKSANAILECVRSADVVSRFGGNQ
                     FAVILDSGDIPLAVSIARRIRKSVGEVIPEFTSVPIGVTRIISGITQQAVLVNADTAL
                     FRAKHSSLDQVAVVDPANPHLEAEHWPNTESD"
     gene            complement(2968..4176)
                     /locus_tag="P5541_03710"
     CDS             complement(2968..4176)
                     /locus_tag="P5541_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71116.1"
                     /translation="MPQRVIAMDKPLAAALEAVKKLETMGHRAAVVGGAVRDLLLCHD
                     IRDADITTSASLASVIEIWPESKLVGRPPDVSAILTHGKSRIDLSSYHGKSLEEDLGR
                     RDLTINAMAMSSDGSIIDPWGGTRDLASGILRFTGNPRKRLEEDPLRCVRLARFASSL
                     PWFEIDPASAIECKQSAPQVRNVHLQRTGREILLSLEGNPALFVGSLESLGLLEAVIP
                     FWDGLPEPEKIITIRRTRIASEMSSDKGIRAFALLASAGDKTREICTSWCWPSRFVKD
                     TENLAKYRNITFGQLDSHIFTVLFREMGPGWIDRLFLAGLIDSISMDDRMDMTMQWSD
                     NRRKTAIWSARLKKNFDIVSGDDIIMVAGKGPGPLIGKILSSIRCHIAEGAVRNKKEA
                     LLLAKKYSEE"
     gene            4279..4761
                     /locus_tag="P5541_03715"
     CDS             4279..4761
                     /locus_tag="P5541_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71117.1"
                     /translation="MFALWMASIILFMVSLFFDRGRSMQAVRNAWDLFLKILPMFLAV
                     LVLTSLSLGFVSEKFLTDILVGKGLFVGTAIAAMAGSVAAIPGFVAFPLAGILAARGV
                     PYTVLGAFTTTLMMVGVVTFPLEKGYLGTRVALVRNLAGFATAIIVSMVIGVFFGEVL
                     "
     gene            4773..5285
                     /locus_tag="P5541_03720"
     CDS             4773..5285
                     /locus_tag="P5541_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRV71118.1"
                     /translation="MIRACLLYGGFGLFSLFSFCSGYEPGLRSIENLKSFVVTMAGIL
                     PPAFLLVGLFNAWVKREVVERHLGENAGFLAYFWAILLAGTMVGGLFVAFPLCHVLRK
                     KGASLAVIFTFLSASAICRVPMTIFEASFLGLKFTIIRYAVSIPLIILSSRIMASWLG
                     EGFVISDGKK"
     gene            complement(5301..5705)
                     /locus_tag="P5541_03725"
     CDS             complement(5301..5705)
                     /locus_tag="P5541_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71119.1"
                     /translation="MSTERICIERKLGSLERCISRLESGQKSSEGKPDEVVALEIGQS
                     LQLCIELASHVISSKLHDQRSGGPAGKFETLAVMGILPHGLATGMKESIELRNIYLNN
                     CRSVGRGQIEPMIATHIRNVRKFAQALSSFQD"
     gene            complement(5722..6741)
                     /locus_tag="P5541_03730"
     CDS             complement(5722..6741)
                     /locus_tag="P5541_03730"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRV71120.1"
                     /translation="MKKITVYGAGSWGTALADLLSRKGHDTTLWCRRDDQARAINTTG
                     RNPFYLSSCDLCPALSASSNLEEAVSASDYHVCAVPTQAVREFFGKVAAFWPENVRIC
                     NVAKGIEIATGERISEIAASVIPGCVYSILSGPSHAEEVIAGKPTAVVVASSVTGEAE
                     KWQEIFSGNLFRAYSSDDVAGVEIGGAVKNVIAIAAGIASARDMGSNALAALASRGLA
                     EVMRLGARSGAHPLTLAGLAGVGDLMVTCFSTLSRNYRLGKAIGEGMTLQEAMDSLGQ
                     VAEGAYTVDALARHARKMGVELPLTEAVYGILYNGIEPEKMLEALFDRELKPELPPEM
                     YWGSR"
     gene            complement(6755..7399)
                     /locus_tag="P5541_03735"
     CDS             complement(6755..7399)
                     /locus_tag="P5541_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRV71121.1"
                     /translation="MLLANRSGVSRLIERLEETPGCRTLSAFLRGLELRDGGNGNGVV
                     LFGDGEILHYERAVDMQPERAAAILEGREYDWAVFHSREASTGSIRDENCHPFVIDGK
                     HHLVMAVNGNETHMSSLSRVIGDKTDSEFIARMIVDMDLPFPETLSCFCSNFIGIYDG
                     KAFAKKGDRQLLKFQSGKDIVFASDFPFSMTEISKPEQNYCWFDWNESILGKDL"
     gene            7556..9553
                     /locus_tag="P5541_03740"
     CDS             7556..9553
                     /locus_tag="P5541_03740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71122.1"
                     /translation="MERNTVLEYIRMVDQAGTVEEACAMAVEAIAGFTRFSNPSFFLL
                     NPGSDSLELVAHHGFTPGTLSIPRGEGISWRTLEKGETALIEDISKVDDYLPGLEGSC
                     CEINVPVVWRERKIGVFSIESKVSGAFSPDDVHFANLLAAILGSVIVHLETENRLSES
                     LRSLESTARYRSLFLEISMEFYSMTDRGEFLERVVRMLSEVMKYDKVYLFLRQTRNGP
                     LQLRAYSGKDIGEKAIRDVVEGGRGLTGKAIRTGEAVFCNDTGNDPDFYLDDEKTLSE
                     AVLPIRSGDYLWGVLVVDEYERGAFEGEEKEQLRILTGMMGVALDNIDQMMRIREDLD
                     LMERLHGIIASVAGERDIRAMCSETVRLLNVDTKYNFVSIQEIVSEKTGESRVIASRA
                     EEGASGYTLVEQNVLLKKGGWGLVGEAARKRSLLNVPDVRKSELYVPLSDKTRSELDV
                     PIEFDGRIYGILSVESEHLFAFEEKDERVMSILARHLGALWAHNELLERTERQAMQDP
                     LTALWNRRFFFDRLDAETSRCARYGCIFSIVMIDLRNFKIVNDRFGHPAGDRVLIEIA
                     SFFQRSLRECDVVARYGGDEFVILFPETDFAEVSRIMERMEENLKNQKLCVESVEVLF
                     DCGIASYPADGKDGRALVQVADERLYSGKNAAKKNCKGGSR"
     gene            9550..9882
                     /locus_tag="P5541_03745"
     CDS             9550..9882
                     /locus_tag="P5541_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV71123.1"
                     /translation="MKHSRNLFVLPDPLPQEELFETLIPDSCVRIERIVSSGQSTPAG
                     SWLEQDDDEWVVLLQGKAVIEYPENIKAELEHGDWIFIPGHTRHRVAWTSSDPPCIWL
                     AVHGRLRK"
     gene            complement(9936..10865)
                     /locus_tag="P5541_03750"
     CDS             complement(9936..10865)
                     /locus_tag="P5541_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRV71124.1"
                     /translation="MSLKKYAKGLLLVTVALSLIAAPAAAKTRIAVIEFEDAAYSGAP
                     ARAITDMLVTELFNTDLFTIIERSKIDTLLMEQNMAATGAIDPSTAVKMGKLLGVELI
                     ITGSITEFRHEAAGGAVPLPGLPGGVAIGSHTAYVTIDTRAIDAQTGEIKMVAREQGA
                     SNQTLGGVAYDGAVFGGGKVGGVMAGATHKAVLKLVERFKEIPSSLPARSETFNVIQA
                     NGPQVMVDAGSNNSSLTPGTFLAAYREGATITAMDGTVLDAEKIYMAILSVVEVKPQY
                     SVCSIIRGIPLERGDKAETLGDRSPESILLGGR"
     gene            complement(10931..>11246)
                     /locus_tag="P5541_03755"
     CDS             complement(10931..>11246)
                     /locus_tag="P5541_03755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV71125.1"
                     /translation="GDRALLQIATSISDSIRSSDMAARWGGDEFILATPAPLEKAKGL
                     AEKLRKLLEELDHDGFGPITGSFGISSFRNGDTIETLTGRADDLMYAVKRLGGNDIRS
                     TP"
     assembly_gap    11247..11346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11347..12251)
                     /locus_tag="P5541_03760"
     CDS             complement(<11347..12251)
                     /locus_tag="P5541_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV71126.1"
                     /translation="MIPDNPERNMGSEENSLYRTLFEISPEGLVFCDQSRRVVLVNDE
                     FCRIFGYERNEAIGRLLDDLVVSGGENLDEARALSEEFRRSGFIQSETVRQRRDGTSF
                     PVFVVAFPVRTGDHLAGSMWIYRNLTEFRKRDEAARIAESKLRGILEAHPELILRFTP
                     DLTVTYANRAYCQYHHIEPEEAIGKSFADHVSPEFIPLVHSKLRSLSPENPAKTGQEK
                     VIMSSGEVRWQEWTDQGIFSDSGELVEIQSVGRDITDRKIMEEALAFERESLSALFEN
                     ASEGIVLCEGDGTIIRANPMFCEMFG"
     gene            12452..13732
                     /gene="eno"
                     /locus_tag="P5541_03765"
     CDS             12452..13732
                     /gene="eno"
                     /locus_tag="P5541_03765"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRV71127.1"
                     /translation="MKTQINTVSAMEILDSRGNPTVRTWCGLSDGTVVSASVPSGAST
                     GENEAVELRDGDKGRYGGKGVLKAVQNVNDIIAPKIRGMDPSRQEDIDRMMIELDGTP
                     NKGRLGANAILGVSMAVARATALSLGVPLYEYLGKREKYPIPVPMMNILNGGKHADNS
                     VDFQEFMVMPTGAPTFAEALRYGAETFHALKKILSGRGYATSVGDEGGFAPNLGSNDE
                     ACEVIVEAIEAAGYTPGKDISLVLDPAASSFYQNGAYDLSKSGQGRKSSSEMTALYRE
                     WIEKYPIVSIEDGLDENDWEGFVHHTSVLGGKIQIVGDDIFVTDPRFVQRGISEKAAN
                     SALIKLNQIGTVTETVETIELCRKAGWGFVISHRSGETEDSFIADFAVAMGGGQIKTG
                     SACRSERICKYNRLLEIERELGEKAFFENPLSRN"
     gene            complement(13775..15271)
                     /locus_tag="P5541_03770"
     CDS             complement(13775..15271)
                     /locus_tag="P5541_03770"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRV71128.1"
                     /translation="MKVKGRLAIVSNRLPLKLERSDENWKVLPSSGGLVSALTPVLRD
                     RGGIWIGWTGTADIVNMKTLREILGPASDRAGYRVFPVLLSREDVDSFYYGFSNEVIW
                     PLFHDLQTMCRFEPEYWNAYRKVNGCFARAVSRHTRTEDFVWVHDYQMIPVARILKES
                     DIHRHCGFFLHIPFPAPDIFLKLPWRDDLLRDLLDYELVGFQTPRDRRNFTDCLKALF
                     PDAKVSGRGPVIRASAEGRQMQVAAMPISIDYRSFRKTAKSREACETLKELRKSISRE
                     VVVLGVDRLDYTKGIPQRIKAFRKFLKDHPEYLNRITFVQIVVPSREDVPAYHELKDE
                     IEKLVSSVNGEFSSPGWVPVHYMYKPVPREELVAYYRLADVALVTPLKDGMNLVAKEY
                     CACQTADDAVLILSEFAGSATQMQRDAVLVNPYDIYGIERALFEAVTMPPDEKKARMR
                     RLRAGIRRQNVFWWVDNYLRATTGKALLDFPETSMAPIFHALGRKSLE"
     gene            complement(15277..16548)
                     /locus_tag="P5541_03775"
     CDS             complement(15277..16548)
                     /locus_tag="P5541_03775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV71129.1"
                     /translation="MGLPYIARLEHYEPFIGTEAVERILLKAEKLSDRRIVHINSTYY
                     GGGVAELLGSMSLLANLAGVQMGWRVIQGSPDFFSVTKKMHNALQGGDINLSWKKFRI
                     YEHVVFENVLRNHIDHDIVVVHDPQPLPMVRHYHKKGPWIWVCHVDLSSPNKKLWPYI
                     RNYLNRYDAAVFSIPEYRQEMDTPLVFIKPAIDPFSIKNREFSEEEIDERLRKYNIPD
                     DKPLIVQVSRFDRWKDPAGVIEAFKIARREVDCNLILLGNIASDDPEGLEIFNELKEL
                     EEEGIMMLTVEDTALVNALQRRAAVILQKSIREGFGLTVTEAMWKGKPVIGGNVGGIK
                     HQITDGENGFLVDSVNEAAERIVMLLKDPDLGRKMGEKARKTVQEKFLMSRYLEEYLD
                     LFKSFEASYRLQKPLWEKRSRSGSRRRPRKV"
     gene            16631..17722
                     /locus_tag="P5541_03780"
     CDS             16631..17722
                     /locus_tag="P5541_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV71130.1"
                     /translation="MTVFRGAKKQGNGIPIIIGQFCEIAKYLARKGSIYGKMNQENYL
                     TGHCSWQREVFILSIVGLGCMILAAFFFGSLGPAERIAFEHGVTPAGVSVVRAVAGAF
                     VVTLYALFRDPGALVPGFRDAWKYALAGLFGVVFVYYLSNIAFVTIPVGLTVILFYTN
                     PIWTMVGSALMGKERFTLARLAALLAGFSGVWLAVGGAGGRAGGSLDPVGVAAAVISG
                     VGYSVYMLNSRYGTGKREPFKTFVQMFLWGAAIMTAIALFRGEMPVFRGMPSTAILSL
                     GHLVVFPTILSYALISYALKYLPGTVASITSMSEILFAGLIAWALLGEVPSGGQVQGG
                     ALIVFAVMLLLVEGNIRAGCGKERVKDQP"
     gene            complement(17749..19548)
                     /locus_tag="P5541_03785"
     CDS             complement(17749..19548)
                     /locus_tag="P5541_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71131.1"
                     /translation="MKTRFFRTLFLTFLLPLLVSGTAWGAMGEITEFSFRSAQEDLLG
                     MEESSEPDGKPDVHLSVSIKGIGAITDVSLKAVDGVREWDTVAGNEKWGIVVKDATGK
                     TISTGSGMPIVPFLGFATLNLYVSDDGTAFSLTREYEVSVKFIDGSTATAKAEIKGIP
                     EMFTPETQVQETGTDAMKAFLYGIRPADVAGKSEAIVADGLDDAHFRVSFSTISVIDQ
                     ITIRNIDGANAMWDTIPGNGVWGIAVFMNDSLKNRPDGSVRFAVEGDTVMDLLVADNG
                     AVAGGKTRFEVIIRFIDGTILRETASTEASSAQPSSEEGFLSAFLFPPSSGDFVGRSE
                     SPGGDGKPDWKVSARISARGTIISLIIRGLDGAPGEWDTLPGNGKPLAAVTDNTGKIL
                     NSPQGSVSIPVEGTMEMNLWLADDGSLSTIANRYRIIAVFSDGRTFERTIDRGTEDPS
                     SEKQQSKAETIQISNSVKAFYMGKGPRNILGKGEPADIRSGLDANPDAHIRLRLLSLE
                     GIIRSITIESLDGKGGDWDTIPGNGIWHIAVTENPTGPVISKTDGSITKSVSGNTDLH
                     LWLADNGKLSANPSNYRVIVRFTDGRALAQNLF"
     gene            complement(19637..20155)
                     /locus_tag="P5541_03790"
     CDS             complement(19637..20155)
                     /locus_tag="P5541_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRV71132.1"
                     /translation="MTSKIAVLVENDFHDIEFWYPFYRLQEAGYESIVVAPAAPTVYK
                     GKYGTTVEAPYDAGGITGETLLGIVVPGGWAPDRLRMSPDIIGLVKRTYSSGGFVAGI
                     CHAGSLLVSCGILSGKKVTSYPSLKDDMILAGAKWIDREVISDERIITSRRPSDLPSF
                     CREILKAASDGR"
     gene            20362..24108
                     /locus_tag="P5541_03795"
     CDS             20362..24108
                     /locus_tag="P5541_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRV71133.1"
                     /translation="MEKKSGELSFGKVIPLSIEEEIRHSYLDYAMSVIIGRALPDARD
                     GLKPVQRRVLYAMLELGLRHNQSYKKSARVVGETMGKYHPHGDSAIYDTMARMAQDFS
                     MRQTLVDGQGNFGSLDGDPPAAMRYCVTGDTLIRSGDSFRKIGNICETEDNTTVETAG
                     ECLTLDMLPGRYDRVFNCGVHPVIELLTESGFRVEGTSNHPLLMIQAGNEGRPELAWS
                     TMDAINEGDYVAMACEEGNFPDELEDKSRDRAIFWGGMISEGYISGDAQRYNRVGFSN
                     TDPEYYDAVKRGFLAEMEHTGNENGNLRESLGHKTKKDVMVLDIDYKPFREAMIGSGY
                     ACRAREKSVPDFVLEGSASFQIWFLRSLFEGDGSCSRLNDKRRGKAYGAVALHSASLE
                     LLRQVQVMLLRFGILGRIYRDKGIHRLLVTSYPDLARFRDVIGFLTEVKNSPLREICE
                     EMKDRSHFGPGSRLKCIPYLARWIREIAVKNRERLQKVNLQDLAGIRKNWDLIQASVP
                     ETDFSYLVGMLRHSFIFQKVSSVRSSGRKRVFSIRVDSACHSFVGNGFINHNTEARLS
                     SAGEQLLSDIDEETVNWGPNFDDSLQEPLTLPTRIPNLLVNGSTGIAVGMATNMPPHN
                     LGEVVDALCLLLDNPDAGTEEVMSVLPGPDFPTGGIILGRGGILDAYRTGRGKIIVRG
                     RAHVEEGRRGKDSIVITEIPFMVNKSNLLEAIAKGVQMKTLDGITDLRDESDRRGLRV
                     VLELARDANPDVTLRQLYRRTQLQSTFGVINLALVDGEPKELSLLSMLGLFIDYRREV
                     VRRRTVFRLRKAEERAHIVEGLIRAIDMIDAVIKLIRGSKDPSEARSGLVEKLGFTEA
                     QAQAILDMRLQRLTNLEKNKLEDEMALLLADIERFRSILANPATLDMVVRDELREVRS
                     NFSDDRRTTIVEAVDDVSVEDLIPEEEIVVVLSRDGYLRRMPLEQYRLQGRGGKGVKG
                     ATPKIGDEAAIITVSTTHRDIFLFTTRGRVFAVRGHVIPEPRTGKGRLASQFVSLEDG
                     ESVVGMRDSHLHGARFVFFITAAGVAKRLPVEELENLTRAGRRVLSVDEGDEISRVRF
                     SSGVDDILIVTAKGQALRVSEGEFRPMGRQARGVRGIRLSADDRVVGCDIVKPGHQVL
                     LVSAQGVGKRTRYDEFMPHHRASGGCRAMKVTDKTGDLIGSWGLMPDDQVIVMTHKGR
                     VIRLGVGDISLLGRTATGYRVIRVAAGDCVADISVIRTSEEEVE"
     gene            24136..24753
                     /locus_tag="P5541_03800"
     CDS             24136..24753
                     /locus_tag="P5541_03800"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRV71134.1"
                     /translation="MQLLLSILAAILSAVFLPLPFAMVFLLLAAFLAWFFRDPERYAR
                     CGDGDWVSPADGKVVEIYPFEHHFTGKCTVVGVFMSPLDVHVNRMPCDGTISWLEYIP
                     GKKLMAFNPKASLENERFLMGFDTAHGRGMTVQVAGFLARRIVCSRSAGERLLRGERF
                     GMIKLGSKVDLYLPEGVVPAVKVGQKVRAGETAIGVMQNEKKKNR"
     gene            24731..25471
                     /gene="pssA"
                     /locus_tag="P5541_03805"
     CDS             24731..25471
                     /gene="pssA"
                     /locus_tag="P5541_03805"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRV71135.1"
                     /translation="MRRRKTDKPIPLRVIIPNMITSGNMLCGMLSLVLAFHGHFVPAS
                     WLIYIAVIFDFMDGKMARRLGGGSSFGMELDSLADVVSFGVAPAMIMYSVYLKGFFGV
                     TGAVVAAFFALCGALRLARFNVQHVDGSFQGLPIPAGGHFLVSFVVAGIYLHPAIMAI
                     VVAAAGVLMISSVPFGNLKALKPGNLDRKKAFFLWAFALTLIVVLRNRAPLTGILIYI
                     ASGFAGVDWGRWLSLENVSEEDLADEEV"
     gene            25510..25761
                     /locus_tag="P5541_03810"
     CDS             25510..25761
                     /locus_tag="P5541_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRV71136.1"
                     /translation="MNSLLGYLIVGAIAGWLAGKVWQGRGFGFFGNLGVGILGAFVGG
                     VIFSSIGLYPMGFIGSLVMAFAGAVVLLFLVGLLRGRRR"
     gene            complement(25835..26338)
                     /gene="thiT"
                     /locus_tag="P5541_03815"
     CDS             complement(25835..26338)
                     /gene="thiT"
                     /locus_tag="P5541_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRV71137.1"
                     /translation="MENRKVRILVEGALAASMALALSYVRLWRMPQGGSITLENIPLL
                     VFSLRWGLRAGFGAGSVAGLLQLILGGYVVHPAQALLDYPLAFGALSLAALIRRPLWL
                     GPVIGSSARFICHLLSGVIFFAAYAPEGTNVWLYSAIYNGSFMLPSLLLSVVAIYLIW
                     PRLGKAY"
     regulatory      complement(26433..26541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26671..27753
                     /locus_tag="P5541_03820"
     CDS             26671..27753
                     /locus_tag="P5541_03820"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRV71138.1"
                     /translation="MLKFFCPRCGKVCEEALRPVRCQCGSPLKSSLDWKNLDIDASAP
                     GMWRYSGVLPPVDRSASMGEGWTPMISGGSRNLFYKLEYVSPTGSFKDRGAAIVMGEA
                     KSLGCGSVVEDSSGNAGASVAAYASRSGIKATIVFPRNVSEQKKRQIELYGAELVEAE
                     GGRKAAAEMAMEMARSTFYASHVWNPLFLEGTRTIAFEIWEQLGKRAPETVIVPAGNG
                     TILLGIFKGFKELLQRVKINRLPKIIAVQSENCCPLFDAWKGTSTEPYRNTVSEGIAV
                     GDPPRMKEMIDAVRISRGDVVTVGDEAVLAERKRLAVEGGLFVEPSAAVAPAAERMLR
                     ESGKITEKETVVVPLTGTGLKGTYKQ"
BASE COUNT         6284 a         7072 c         7797 g         6702 t
ORIGIN      
        1 ctcacctctc aaccctcaca cccctgccct caagcgcgac cagaaccact actgccatta
       61 ccattgccat ggcggctccc gtgaaaaaaa ccgccctgat gccgaaaccg gagaatatca
      121 taccaaacat gataggccca acggtttgac ccaggcggag agacatgctg tttattgaaa
      181 gaaacatggc cctgtccttt tcgggggagt tttcagtgag catgctcaga agtgccggta
      241 tgttgatccc ctgacctatt ccgaaaagga cggcaggaac cagcatgcca aggcatccgg
      301 gaacgtgcag gatgaaaaat agtgctgcgc cataaataaa gaatgaggat gctataaaaa
      361 gcggtcctct ttccatgcgg aaccagcgac cggcgatcgg tgctgtgatt gcgctggata
      421 tggacatgga agaggatacg aggcctatca tcaggggggt tgcggcaaag gtcgaagcca
      481 ggaaaaaggg aagaaaagta aggaaacttc catactggac gataaaggac atcatgctgg
      541 ctgcaaggat aacgggtgtg ccctttgatc cgatgctttt tgccagagag gacagatctt
      601 tcccaatctc gatgcggccg gttttggtca gttttttctc ctcaagcccc agcagcaccc
      661 aggtgccgac ggggagggcc aggaacgcga gaagaaaggg gtagttccag ccgatcatgg
      721 ccaacagtcc tcccagggcg gggtagctcg cagttcctgc gccgatagcg cttgtgttgt
      781 aaccgatggc ggcagatctt tcctttccgt agaaaaggtc cccgatcaga gccacgttga
      841 gagagttaag aggggcagat ccgaggccct gaaggaaacg cagcataaga agggttctga
      901 aatccggtgc aaaaaaacaa cctccgcccg cgaggccgaa aagtaacaga catgggaaca
      961 ggatccgctt ccgcccgtaa cgatccgaaa gggctccggt aattggggtg aagaggagcc
     1021 cggggatcgt aaacatggag atgagccacg ccgcatcctc gcgggggacc ctcatggcgg
     1081 acgttatctg cggaaaagca ggggcaatgg aggctactcc caggacggag ataagggtta
     1141 tgccgaagat gatgttcaga tttgggtcaa aaaggatgga atggtccctg gatgtttcct
     1201 tcatatcggg gatcgaagcc cctctcttgt ttcctaccag tggttctcgt ggatcctgtc
     1261 agggtcgaat cgcttcgagg ggcctttctc ttctgccggg taaccgagag caacgaaaca
     1321 gaagggagtt atttcgacgg ggatcccgca aacctgtcgg gacttttcaa caagatcctg
     1381 gtggggaaat atccccagcc agacagagcc aagacccagg gagtttgccg cgaccaacat
     1441 gttctgggtt gctgcggcac agtcctgagg ccagaaaccc tctgcgggga gcccctcagt
     1501 cctggagcag accatgatgg ctacgggtgc ctgaaggagc atctttgaat atgggtggaa
     1561 ggagggtatt gaatcaagaa tttctctgtc tgttattacc acgaactccc atggctgctg
     1621 gttatgagca ctgggggcgc tcattgccgc cttgagaagg gtttcgaccg ttgcttcgtt
     1681 gacggtttta ccggagaact ttcttatgct tcttctggaa aggatggcgt cgatggcttt
     1741 ggtctccacg atcttttccc ccttcgatgg aatctgtttt caatcggatt ccgtgttcgg
     1801 ccagtgttcc gcttcaaggt gaggattagc cgggtcgacc accgctacct ggtcgaggct
     1861 tgagtgtttt gccctgaaaa gcgccgtgtc ggcattgacc agaacggcct gctgggttat
     1921 gccggaaata atccttgtga cgccaatggg gacggaagtg aactccggga tcacctctcc
     1981 aacggatttt ctgatccttc gggctatgga gacggcaagg ggtatgtccc cgctgtcgag
     2041 tattacggcg aactggttgc cgccaaacct tgaaaccaca tctgccgagc ggacacattc
     2101 aagtattgcg ttggctgatt ttttgaggaa tgcatcgcct acaaggtggc cgtgtgcctg
     2161 gcaaacgttc tggtagtcgt cgaggtctat gaaaagcagg gttctatctg tctcgttctc
     2221 gataaggatc ctcagcaggt tatcggtata ccttctgttg tagacacctg taaggtcgtc
     2281 cctcagtgcg gcgtcctcgg cgttcttttt tgccgtcagg agagaggaga cgtgggttac
     2341 gttcatgcgg agaatatcca ggaaaaagta cgagaaaaga agggtgaagg agtatgtcac
     2401 cactatatat cccatcatca tgtccagttc cctcaggtgg aggatggggt aatagaagca
     2461 ggcagcgtta taaaggagta tcaggaagaa ggcaaaacct ttgggctgga tgaaatatgg
     2521 gcctatcagg gtggaaaaga gcagggtcat tacctgtctg agaaggataa tctccaccgg
     2581 tatgccaagg tgtacaaaga aaacaggaaa ggatgagatc aatatcaggt tcaggatcgc
     2641 gagccatgcc agggcggatc gtttgtgaaa gggaaacagc atcgccgtcg cgggagcaca
     2701 cgcaaggtat gccagcgcaa gggctccgtc gatcttttgc atgaacgtgt agccaaagag
     2761 cacgtcagta aggaacatgg tggcgaacca tcccgcaagc accaaacagg tgaccagctt
     2821 tcgtcttctc tgctgtctct ggaattcgac agccaccttg cccatttcga cctgagtcat
     2881 gacggaaccc ccgtatgcag agtaaaaaca tattgacctg acttacagaa tactatagtt
     2941 cggtttgata atcaaagaaa gcccagttca ttcttcagag tatttttttg cgagcaacag
     3001 ggcttctttt ttgtttctta ccgcgccttc agcgatatgg caacgtatag acgaaaggat
     3061 ctttccgatg agaggacctg gtcctttccc tgccaccata attatatcgt ctccggagac
     3121 gatgtcgaag ttcttcttga ggcgagcgga ccagatagct gtctttctgc ggttatcgga
     3181 ccactgcatt gtcatgtcca tccggtcgtc catggatatc gaatctatca gtccggccag
     3241 aaagagcctg tctatccacc ctggacccat ctctctgaaa agcaccgtga aaatgtggct
     3301 gtcgagttga ccgaaggtaa tattcctgta ctttgcaagg ttctctgtat ccttgacaaa
     3361 acgggacggc cagcaccagg aggtgcatat ctcccttgtc ttgtcccctg ccgaggccag
     3421 aagggcaaat gctcttatac ctttgtcgct gctcatttca gatgcgatcc tagtcctgcg
     3481 gatagtaatg atcttttcgg gttctggaag tccgtcccag aaaggaatga ccgcttccag
     3541 aaggccaaga gattcgagtg accccacgaa aagggccggg tttccttcga gagacaaaag
     3601 gatctccctt ccagttctct gaaggtggac attcctgacc tgtggggcag attgcttgca
     3661 ttcgatggcg gaagcgggat ctatctcaaa ccatggcaac gatgaggcaa aacgtgcaag
     3721 gcggacgcac ctcagtggat cttcctcgag gcgtttacga gggttgccgg tgaagcgaag
     3781 aatacccgaa gcgaggtccc tggtgcctcc ccacggatcg atgatactgc cgtcagaaga
     3841 catggccatg gcatttatcg tcaggtctct cctgcccagg tcctcttcca ggctcttgcc
     3901 gtgataagac gaaaggtcta tacgggattt gccatgggtc aggatcgctg aaacatccgg
     3961 aggtctgccg accagttttg attccggcca gatctcgata actgatgcca gagaggccga
     4021 cgtagtgata tcggcatctc taatgtcatg gcaaagcagg agatcccgaa ccgcgcctcc
     4081 gacgacggca gcacggtgcc ccatggtttc cagttttttt actgcttcaa gcgcagcagc
     4141 aagaggcttg tccattgcta tcaccctctg gggcaatttt atacccttcc agatgagagc
     4201 gatattattg gaacagaggg atcgagagat gctatattta tcccgagcct gaatttttgg
     4261 aaaaaagggg gattattttt gttcgcctta tggatggcct caataatttt gttcatggtc
     4321 tccctgtttt ttgacagggg cagatccatg caggctgtca ggaacgcctg ggacctcttc
     4381 ctgaagattt tgcccatgtt tctggcggtg cttgtcctta cttcactgag cctgggattt
     4441 gtaagcgaga agttccttac ggatatcctg gtcggaaagg ggctctttgt cggaaccgcc
     4501 atcgcagcta tggcaggctc ggtggctgcc atacccggct tcgttgcgtt tcccctggcc
     4561 gggatacttg ccgcccgggg ggtgccctac actgttctgg gggccttcac aactacgctg
     4621 atgatggtgg gagtcgtcac tttcccgctc gaaaagggat atctgggaac aagggttgcc
     4681 ctggtaagaa accttgcggg tttcgcgacg gccattattg tatcaatggt cataggggtc
     4741 ttcttcggtg aagtattgtg aaaagaggta aattgataag ggcgtgcctt ctttacgggg
     4801 gctttgggct tttcagtctg ttttcttttt gttccggtta cgagcccggt ttgaggagta
     4861 ttgaaaacct caagtccttc gtggttacca tggcagggat attgcctccc gcattccttc
     4921 tggtggggtt gttcaatgcc tgggtgaaac gggaggtggt tgagagacac cttggtgaaa
     4981 acgccggatt cctggcatat ttctgggcta ttcttcttgc cggaaccatg gttgggggtc
     5041 ttttcgtggc tttccctctc tgtcatgtac ttcggaaaaa aggcgcatcc ctggctgtga
     5101 tcttcacgtt cctgagtgct tcggccatat gccgtgtccc catgaccata ttcgaggcct
     5161 cgtttcttgg gctgaaattc acgataatac gttacgctgt atcgattccc ctgattattc
     5221 tttcttctcg aataatggcg tcatggctcg gagaagggtt cgttatatcc gacggcaaaa
     5281 agtgacccgc attatttccg tcaatcctgg aaggaggaaa gtgcctgggc gaatttgcga
     5341 acgttcctga tgtgggttgc gatcatcggt tctatttgcc ctctgccgac cgaacggcag
     5401 ttgttcaggt aaatattcct cagttcgatg ctttctttca ttccagtcgc aagtccgtga
     5461 ggtagaatac ccataaccgc aagggtttca aacttcccgg ccgggccgcc cgatctctga
     5521 tcatgcagtt ttgacgatat tacatgcgat gccagttcga tacatagctg gaggctttgt
     5581 ccaatttcaa gggccacaac ttcgtcaggc tttccttcag aggatttctg ccccgactcg
     5641 agccttgaaa tgcatctttc cagagatccg agtttccttt cgatacagat cctttctgtt
     5701 gacacgttca tacccccctt attatctgct tccccagtac atttcgggag gaagttccgg
     5761 tttaagttcc ctgtcgaaaa gagcttccag cattttctcc ggttcgatac cattataaag
     5821 gatcccgtat actgcctcag tcagcggcaa ctccacgccc atctttctgg catgccttgc
     5881 cagggcatca acagtatagg ctccctccgc gacctggccc agtgaatcca tggcttcctg
     5941 aagggtcatt ccctccccta tggcctttcc aaggcgataa ttccgggaaa gtgtgctgaa
     6001 gcacgtgacc atgaggtctc caactccggc cagaccggcc agggtgagag ggtgagctcc
     6061 gcttctggct ccaagtctca taacctcggc aagacccctg ctggcgaggg cagcaagggc
     6121 gttgctgccc atgtcgcgcg cgctggcgat acctgccgcg atagcgataa cgtttttaac
     6181 ggcccccccg atctccacac ctgcgacatc gtcgctggag taagcccgga aaagatttcc
     6241 ggagaatatc tcctgccact tttctgcctc tccggtaaca gacgaggcta cgacgacagc
     6301 ggtcggcttg cccgctatga cctcttcggc atggctgggc cccgagagga tgctgtaaac
     6361 gcatccaggt atcaccgagg ctgctatctc gcttatcctc tccccggttg ctatctctat
     6421 gccctttgcg acattgcaga ttctgacgtt ctctggccag aaagccgcca ccttgccgaa
     6481 aaattccctg acagcctgtg tgggtaccgc gcatacatga taatccgatg cagaaaccgc
     6541 ctcttcaaga ttcgacgagg ccgaaagtgc cgggcagaga tcgcaggagg agaggtaaaa
     6601 agggtttctc cccgtggtat tgattgccct tgcctggtca tcccttctgc accatagagt
     6661 tgtatcgtgt ccttttcttg aaagaagatc ggcaagggcc gtgccccagc ttccggctcc
     6721 gtaaacagtg attttcttca tcttttctcc ttcatcataa atctttgccc aggatggact
     6781 cgttccagtc gaaccagcaa tagttctgct ccggtttgga tatttccgtc atagagaagg
     6841 gaaaatcgct ggcgaagacg atgtctttcc ccgactggaa tttgagaagc tgccgatctc
     6901 cctttttggc aaaagccttg ccgtcgtaaa tgccgatgaa attggagcag aaacatgaaa
     6961 gggtctcagg aaaagggaga tccatgtcca ctatcatccg ggctatgaac tcgctgtcgg
     7021 ttttgtcccc tataaccctg cttagagagg acatatgcgt ctcgttacca ttgaccgcca
     7081 ttacaaggtg gtgtttgcca tcaataacga aggggtggca attttcgtcc ctgatggatc
     7141 ctgtgctggc ctccctggag tggaagacgg cccaatcgta ctctctccct tcgaggatag
     7201 ccgccgctct ttcgggttgc atgtccacgg ctcgctcgta gtgaaggatc tcgccatcgc
     7261 caaacaggac tacgccgttg ccgttgccac cgtccctgag ttccagcccc cgcaggaaag
     7321 ccgaaagtgt tctgcacccg ggcgtctctt ccagcctttc tataagtctc gaaacgcccg
     7381 atctgttagc cagaagcatc ctgcacaacg ggatcacccc tttttggagc aagttatcgc
     7441 tcttgtgatg tcaatcatat aacagtgaat gcccgaatag aagcacctgt ttcgggaaca
     7501 tggtgataaa ataatccagt gtgataataa gacgatggaa caagagggtg gctttgtgga
     7561 aagaaatacc gttctggaat atatcaggat ggtcgaccag gctgggacag tagaagaggc
     7621 ctgcgcaatg gctgtcgaag ccatagcagg ttttacccgc ttttccaacc cctcgttttt
     7681 cctcctgaat cccggtagcg attccctgga actggttgcc catcacggtt tcacacctgg
     7741 aactctttca atcccccggg gtgaggggat ctcctggaga actctcgaaa agggagagac
     7801 agccctgatt gaggatatct cgaaggtcga tgattacctt cccggccttg agggatcatg
     7861 ttgcgagatc aatgttccgg tggtgtggcg ggaaagaaag ataggggtat tcagcataga
     7921 atcaaaggta agtggcgcct tttcccccga tgatgtacat tttgccaatc tgcttgccgc
     7981 cattctcggg agcgtgatcg ttcacctgga aacagagaac cgcctgagcg aaagtctcag
     8041 gtcgctggaa tccacagcaa ggtacaggtc cctgttcctg gaaatatcaa tggaatttta
     8101 ttccatgacc gacagaggag aatttcttga aagagtagtc aggatgttgt cggaggtaat
     8161 gaaatacgac aaggtgtatc ttttcctcag acagacaaga aatgggccac tgcaactcag
     8221 ggcgtacagt gggaaggata ttggtgaaaa ggcgataaga gatgtagtgg aaggcggcag
     8281 aggcctgaca ggaaaggcga taaggaccgg tgaagcagtt ttctgcaacg atacaggaaa
     8341 tgaccccgat ttctatcttg acgacgaaaa aaccctttcc gaggccgttc tccccatacg
     8401 ttcgggggac tacctgtggg gagttctcgt ggtcgatgaa tacgaaaggg gcgcattcga
     8461 gggagaggag aaagaacagc tcaggatcct gaccgggatg atgggtgtag ctctggataa
     8521 tatcgaccag atgatgagga tcagggagga tctcgacctg atggaaagac ttcacggaat
     8581 aatcgcgtct gttgccgggg agcgcgatat cagggcaatg tgcagtgaga ccgtgaggct
     8641 tttgaacgtt gacacaaaat acaattttgt cagtattcaa gaaattgtct ccgaaaaaac
     8701 cggcgagagc agggtcatcg ccagccgcgc tgaagagggg gcctcggggt atacccttgt
     8761 agaacagaat gtccttctca agaaaggagg atggggcctg gtaggggagg cggcaaggaa
     8821 gaggagcctt ctcaacgtcc ccgatgttcg gaagtcggaa ctctacgttc cgctgagtga
     8881 caagacccgg tctgaactcg acgtgccgat tgagtttgac gggagaatct acggtattct
     8941 ctctgtggaa agcgaacatc tctttgcctt cgaggaaaag gacgagagag tgatgtcgat
     9001 ccttgccaga catctcgggg cgttgtgggc tcataatgaa cttcttgaac ggaccgaaag
     9061 gcaggcaatg caggatccgc tcacggccct ctggaaccgc cgattcttct tcgacaggct
     9121 cgatgccgag acttcacgat gcgccaggta tggctgtatt ttcagcatag tgatgataga
     9181 cctcaggaac ttcaagatcg tcaacgacag atttggccat cctgcgggcg acagggtcct
     9241 gatagagatc gcctcattct tccagagatc tttgagggaa tgcgacgtag ttgcaaggta
     9301 cggtggagac gagtttgtaa tccttttccc ggagaccgat tttgccgagg tttcgcgcat
     9361 catggagcga atggaagaaa acctgaaaaa tcagaaactc tgcgtcgaat cggttgaagt
     9421 acttttcgat tgcggcattg cttcctatcc cgcggacggc aaggatggaa gggcccttgt
     9481 tcaggtggcg gacgaaaggt tatacagtgg gaaaaatgcc gcgaagaaaa actgcaaggg
     9541 cggttccagg tgaagcactc ccgcaatctc ttcgtcttgc ctgatccact tccgcaagaa
     9601 gaacttttcg agaccctgat ccctgattcc tgtgtaagga tagagaggat agtttcctcg
     9661 gggcaatcaa ccccggccgg gtcctggctc gagcaggatg atgatgaatg ggtcgttctc
     9721 ctccagggaa aggccgttat tgaatatccc gaaaacataa aagccgaact ggaacatgga
     9781 gactggatat ttattcccgg tcacaccagg catcgcgttg cctggacatc ctccgatccc
     9841 ccctgtatct ggctggctgt ccacggcagg ctcagaaaat agaaaagggg ccgggccgcc
     9901 gggcccaacc ccctgaagca aaggcttttt cagtgctacc tgcctccgag aagtatgctc
     9961 tccggggacc tgtcaccgag agtctcggcc ttgtcgcccc tctcaagggg tatccccctg
    10021 atgatggaac atacgctgta ctggggcttg acttccacga ctgaaagaat cgccatgtat
    10081 atcttctcag cgtcgagaac tgtgccatcc atcgccgtga tggtcgcacc ctcgcggtag
    10141 gccgcaagga atgttccagg agtaaggctg gagttgttgc tcccggcatc caccatgacc
    10201 tggggaccgt ttgcctgtat cacattgaag gtctccgacc tggcaggcag ggatgagggt
    10261 atctccttga acctctcgac gagtttgagc acggccttgt gtgtggcgcc agccatgact
    10321 cccccgacct ttccgccgcc aaagacagct ccgtcgtatg cgactccgcc aagggtctgg
    10381 ttggaagccc cctgctcgcg tgccaccatt tttatctctc ccgtctgggc gtcgatggcg
    10441 cgggtgtcaa tggtcacgta ggcggtgtgg gagccaatgg caacgcctcc ggggaggcct
    10501 gggagaggaa ccgctcctcc cgcggcttca tggcggaact cggttataga tccggtgatt
    10561 ataagttcca caccgagaag ttttcccatt tttacagccg ttgaggggtc gatggctcct
    10621 gtcgcggcca tgttctgctc cataaggaga gtgtcgatct ttgatctctc gattatggta
    10681 aacagatcag tattgaaaag ttccgtgacg agcatgtctg ttattgctct tgccggagcc
    10741 ccggagtagg cggcgtcctc gaactcgatt actgctatcc tggtcttcgc cgcggcagga
    10801 gccgcgatga gagagagagc cacggtaacc agcaacaggc cttttgcgta ttttttcaga
    10861 ctcatttgtt ttctctcctt ttatttatgg ttacaggcag atacatcata ccagtcccgg
    10921 tgaagggatt ctacggggtg gacctgatgt cgttccctcc aaggcgtttc acggcataca
    10981 tcaggtcatc tgcccttccg gtgagggttt ctatggtgtc gccatttctg aaagatgata
    11041 ttccgaaact tcctgttatg ggtccaaacc cgtcgtgatc aagttcttcg agaagttttc
    11101 tgagtttttc cgccagcccc tttgcctttt ccagcggggc cggcgtcgcg agaatgaact
    11161 catcccctcc ccaccttgcg gccatatcag aggagcgaat gctgtcgctg atcgaagttg
    11221 caatctgtaa aagggccctg tcgccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnccga acatttcgca gaacatgggg ttggccctga ttatggttcc gtctccctcg
    11401 cagaggacta ttccctccga agcattctcg aaaagggcgc tgagggactc tcgctcaaag
    11461 gcaagagcct cctccatgat cttgcgatcc gttatgtccc tgccgacgga ctggatctct
    11521 acaagttcgc cgctgtcgct gaatatcccc tggtcggtcc actcctgcca tcgaacctct
    11581 cccgaggaca ttattacctt ttcctgacct gtcttggcgg ggttttcggg ggagagggac
    11641 ctgagtttcg agtgaaccag ggggataaac tcgggcgaaa catgatcagc gaaggacttg
    11701 ccaatggctt cttcaggttc tatatggtgg tactggcagt atgccctgtt ggcatatgta
    11761 accgtaaggt cgggtgtgaa acggaggatc agttccgggt gggcttcgag tatcccccga
    11821 agtttggatt cggctattct tgctgcctca tcccgcttgc ggaattccgt caggttcctg
    11881 tatatccaca tcgaaccggc aaggtggtct ccagtcctta cagggaaggc aacaacgaaa
    11941 acagggaagg aggttccatc tcttctctgg cgaacagtct cagactgaat aaaaccggaa
    12001 cggcggaatt cctcactgag ggccctggct tcatcgaggt tttcgccccc acttacaacc
    12061 aggtcatcta gaagtctccc tatggcctcg tttctctcgt agccgaagat ccggcaaaac
    12121 tcgtcgttga caaggacaac ccttcttgac tggtcgcaaa aaaccagccc ctcgggggat
    12181 atctcgaaca gtgtcctgta aagggaattc tcttctgatc ccatatttcg ttcaggatta
    12241 tcaggaatca aaggttttct cctctcctgt cagtggaagg gcaggataaa catactgatt
    12301 atatcattat tcttatcgcc cgtgtttttt cgaaagaaat atagagaagg ttgaggtaat
    12361 aatcgttaat ttttgatata gtctggtata gactttcact gaggcggtga ttccgccctc
    12421 ggaatatgaa aaaagcgaag gggagagggt aatgaaaacg cagatcaaca ccgtaagcgc
    12481 aatggagatc cttgactcga gggggaaccc gacggtccgg acgtggtgcg ggctttctga
    12541 cggaaccgtc gtttcggcct ccgttccttc cggtgcctcc acgggggaga acgaggcggt
    12601 ggagctccgt gacggggaca aggggcgcta cggaggcaag ggtgttctta aggcagtgca
    12661 gaacgtaaat gatattattg ccccgaagat cagggggatg gatccgtccc gacaggaaga
    12721 tattgaccgg atgatgatag aactggacgg gactcccaac aagggcagac tgggagccaa
    12781 tgccatactg ggtgtttcca tggcagttgc cagggcgacg gcgctctctc tcggagttcc
    12841 tctttacgaa taccttggca aaagagaaaa gtatcccatt ccggttccga tgatgaatat
    12901 cctcaatggc ggcaaacatg ccgataacag cgtggacttc caggagttca tggtcatgcc
    12961 cacaggcgcc ccaacctttg ccgaggctct caggtatggc gctgagacct tccatgccct
    13021 gaaaaagatc ctgtcgggaa gaggttacgc gacaagcgtg ggcgatgagg gaggtttcgc
    13081 gcccaacctt ggcagcaacg atgaggcatg cgaggtgatc gtggaagcca ttgaggcagc
    13141 gggctatact cccggcaagg atatctccct ggttcttgac ccggctgcca gctcctttta
    13201 tcagaacggg gcctacgatc tttccaagtc gggacagggg cgaaagagca gttccgagat
    13261 gacagcactt tacagagaat ggatagaaaa gtaccctatc gtctccatcg aggatggcct
    13321 tgacgagaat gactgggagg gtttcgtcca tcacacgtct gtcctgggtg ggaagatcca
    13381 gatagtcggc gacgatattt tcgtaaccga tcccaggttt gtccagaggg ggataagtga
    13441 gaaggccgca aactcggccc tgataaaact gaaccagata ggtacagtga cggagaccgt
    13501 ggagacgata gaactgtgcc gcaaggcggg ttggggcttc gtgatctccc accgcagcgg
    13561 cgagacagag gactcattca tcgcggattt tgccgtcgcg atgggtggag ggcagatcaa
    13621 gaccggttcg gcttgcagga gcgagaggat ctgcaaatac aatcgtctcc tcgaaattga
    13681 gagggaactg ggggaaaaag ccttttttga aaatccctta tccaggaact gacgggatga
    13741 tccctggaat attgccggca gcgcttcatt ggcattattc cagggatttt cttcccaggg
    13801 cgtggaagat cggagccatc gatgtttccg ggaaatccag cagcgccttc cccgtggtcg
    13861 ccctcaggta gttgtcgacc caccagaaca cgttctgcct gcggatgcct gcccttaatc
    13921 ggcgcatccg cgcttttttt tcgtccgggg gcattgtcac tgcctcaaaa agagcccttt
    13981 ctattccgta gatgtcatag gggttcacca gaacggcatc gcgctgcatc tgtgtggcgc
    14041 tccccgcgaa ctcgctgagg atcagaacag catcgtctgc cgtctggcat gcgcagtatt
    14101 ccttcgcaac gaggttcatc ccgtccttca ggggagttac cagggctaca tcggcgagcc
    14161 tgtaatatgc caccagttcc tccctgggta ccggtttgta catatagtgg acagggaccc
    14221 atcccggtga tgagaactct ccgtttacgg aactcaccag tttttcgatc tcgtccttca
    14281 gttcgtgata tgcgggaacg tcttccctgc tcgggaccac gatctggacg aaagtaatcc
    14341 tgttaaggta ttccgggtga tccttgagaa acttcctgaa ggcctttatt ctctggggta
    14401 tccccttggt atagtcaagc ctgtcaaccc cgaggacgac tacctctcga gagatgctct
    14461 tcctgagttc cttgagagtt tcacaggctt ccctggattt tgcggtcttc ctgaaggacc
    14521 tgtagtctat gctgataggc attgccgcga cctgcatttg tcttccttct gcggaagctc
    14581 tgatcacggg tcccctcccg gacacctttg cgtccgggaa gagagctttc agacagtccg
    14641 tgaaatttct tcggtccctc ggagtctgga agcccacaag ttcgtaatcc aggagatccc
    14701 tcaaaaggtc gtcccgccag ggcagcttca gaaagatgtc aggggcggga aaaggtatat
    14761 gaaggaaaaa cccgcaatgt ctgtgaatat cggattcctt gagtattcgg gccacgggta
    14821 tcatctggta gtcatggacc catacaaaat cttccgtcct ggtgtgtcgg gagaccgccc
    14881 gggcgaagca gccattcacc ttcctgtagg cgttccagta ctcgggctcg aagcggcaca
    14941 tggtctgaag atcatgaaaa agcggccaga tcacctcgtt ggaaaaaccg tagtagaaag
    15001 aatccacatc ctctctggat aaaagaacgg gaaaaaccct gtaacccgcc ctgtcggatg
    15061 ctggtcccaa gatctcccgc agcgttttca tgttcacaat atcggctgtt cccgtccatc
    15121 cgatccagat acctcctctg tcgcggagaa cgggagtcag cgccgaaacg agcccccctg
    15181 aagaaggcag gaccttccag ttttcgtctg atctctccag tttcaggggg agcctgttcg
    15241 agactatcgc gagtcttccc ttgaccttca tcgggtctac accttcctgg gccttcgccg
    15301 tgagcctgac cgggaacgct tttcccacag gggtttctgg agcctgtaac tcgcctcgaa
    15361 ggatttgaaa agatcaaggt attcttccag gtacctggac atcaggaact tctcctgtac
    15421 cgttttgcgc gccttttctc ccattttcct cccgaggtcc gggtccttga ggagcatcac
    15481 tatcctttcg gccgcttcat tgacagaatc taccagaaaa ccgttctctc catcggtgat
    15541 ctggtgtttt atacccccca cattgcctcc gatgacaggt ttgcccttcc acatagcttc
    15601 ggtcacggtc agcccgaagc cttccctgat cgacttttgc agtattaccg ccgccctgcg
    15661 ctggagggcg ttgacaagag ccgtgtcctc aacggtgagc atcattattc cctcttcctc
    15721 gagttccttc agttcgttaa atatttccag gccctcgggg tcgtcggatg caatattccc
    15781 gaggaggatc aggttgcagt cgacctcacg acgggctatc ttgaaggcct cgatcacccc
    15841 cgcggggtcc ttccacctgt cgaaacgtga gacctgaacg atcagcggct tgtcgtcggg
    15901 aatgttgtat ttcctgagcc tttcgtctat ctcttcttcg gagaactcgc ggttcttgat
    15961 gctgaatgga tctattgccg gttttatgaa aaccagcggg gtgtccatct cctgcctgta
    16021 ttcgggtatg ctgaagacgg cggcatcgta cctgttgaga tagttcctta tatagggcca
    16081 caattttttg ttggggctgg aaaggtcgac gtggcacacc catatccacg gtcccttctt
    16141 gtgatagtgt ctcaccattg ggagcggctg ggggtcatga accacaacaa tatcgtgatc
    16201 gatatggttg cggagcacgt tctcgaaaac cacatgttcg tatatcctga actttttcca
    16261 actgaggttg atatctccgc cctggagggc gttgtgcatc ttcttggtga cgctgaaaaa
    16321 gtcgggagag ccctggatca ccctccaccc catctggact cccgccaggt ttgcgagcag
    16381 gctcattgag ccgagtagtt ctgccacacc cccgccataa tatgtggaat tgatgtggac
    16441 tatccttctg tcagacaact tctcagcctt cagaaggatc ctctcgacgg cttcagtgcc
    16501 aatgaagggt tcgtaatgtt ctaaccgggc gatgtatgga agccccattc ctgatcactc
    16561 ctttcctggg gttttcgtaa aaaatggatc aggggaaaat gaggactgtc gaatcgtttt
    16621 gagatattga atgactgtat ttcgaggggc gaagaagcag ggaaacggaa tacccattat
    16681 tataggacaa ttttgcgaaa ttgcgaaata cctcgcccgc aagggctcaa tctatggcaa
    16741 aatgaaccag gaaaattatt taactggcca ttgttcctgg caaagagagg tttttattct
    16801 gtctatagtt ggtttgggtt gcatgatcct tgcggctttt ttctttggct ccctggggcc
    16861 tgccgagcgt atagcctttg agcacggtgt tactccggcg ggagtatctg ttgtcagggc
    16921 cgtcgcggga gcttttgtcg taaccctgta tgccttattc cgggatcccg gggctctggt
    16981 tcccggtttc agagatgcct ggaaatatgc tcttgctggt ctcttcggtg tagtttttgt
    17041 gtactatctc tccaatattg ccttcgttac catacctgtt ggcctcaccg ttatcctgtt
    17101 ctacacaaat ccgatctgga ccatggtcgg ttccgccctg atggggaagg agcgtttcac
    17161 ccttgcccgc ctcgctgccc tgctggccgg tttctccgga gtatggctcg ccgtgggggg
    17221 agcggggggc cgcgcgggtg ggtcactcga tcccgtgggt gttgccgcgg cagtgatctc
    17281 cggagtggga tactcggtct atatgctcaa cagcaggtac ggaaccggca agagagagcc
    17341 cttcaagacc ttcgtccaga tgttcctctg gggggcagcg ataatgactg caatagccct
    17401 tttcagagga gagatgccgg tttttcgggg aatgccatcc accgcgatcc tctccctggg
    17461 acatctggtt gtattcccca cgatcctttc atacgctctt atatcctatg cgctgaaata
    17521 tcttccaggc actgtagcct cgatcacttc catgtcggag attcttttcg ccggattgat
    17581 agcctgggcc cttctcggag aggtgccatc ggggggtcag gtccaggggg gcgccctcat
    17641 tgttttcgcg gtgatgcttc ttctcgtgga aggcaatatc cgggccggtt gcggaaaaga
    17701 gagggtgaag gaccagccct gagggtgatc cttcaccggt aattactcct agaagaggtt
    17761 ctgtgccagg gccctgccgt cagtgaaccg gacgatcacc ctgtagttgg acgggtttgc
    17821 gctcagtttg ccgttgtccg cgagccagag gtggagatct gtgttgcccg agaccgattt
    17881 tgtgatcgaa ccgtctgtct ttgatataac cggtcccgtc ggattttctg ttacggcaat
    17941 atgccatatt ccgttaccgg ggatagtgtc ccagtctccg cctttgccat cgagggattc
    18001 aatggttatg gatctgatga tcccctcgag agaaagtagc ctgagcctta tgtgggcatc
    18061 gggattcgcg tcaaggccgc ttctaatgtc ggccggttcc cccttgccca ggatattcct
    18121 gggtcccttc cccatgtaaa aggcctttac gctgtttgat atctgtattg tttccgcttt
    18181 tgactgttgt ttctcactgg aaggatcttc cgttccccga tctatggtcc tctcgaaagt
    18241 cctgccgtca ctgaagaccg cgattatcct gtaacggttc gcgatcgttg acagtgatcc
    18301 gtcatcggcc agccacaggt tcatctccat tgtgccttcg acaggtatcg agacggagcc
    18361 ctggggggag ttgaggatct tgcctgtatt gtcagtaacg gcggccagtg gtttcccgtt
    18421 gccgggaagt gtgtcccatt cgccgggggc accgtcaagg cctctgataa taaggcttat
    18481 gattgttccg cgggcggaga tccgggccga aaccttccag tccggcttgc cgtcaccgcc
    18541 aggcgactcg ctcctgccga caaaatcccc ggaggatgga ggaaagagga aggcagagag
    18601 gaacccctct tcactggaag gttgcgctga ggaagcctct gtagaggctg tctccctgag
    18661 tatggttccg tcgatgaagc ggatgataac ctcgaacctt gtcttgccgc cggctacggc
    18721 tccgttgtcg gccaccagca ggtccataac agtatctccc tcaacggcga aacggacgct
    18781 tccgtcgggc ctgtttttca ggctgtcgtt catgaatacc gcgatacccc agactccatt
    18841 tcccggtatg gtgtcccaca tggcgttggc tccatcgatg ttgcggatcg ttatctggtc
    18901 aattacagaa attgtcgaga aagagaccct gaaatgagcg tcgtcgagtc catcggcgac
    18961 gatggcctca ctctttccgg caacgtccgc gggccggata ccgtatagaa atgctttcat
    19021 ggcatcagtt cctgtttcct ggacctgagt ttccggggtg aacatctcgg gtataccctt
    19081 tatctctgcc tttgcagttg ccgtactgcc atcgataaat tttaccgaaa cctcgtattc
    19141 cctggtcagt gagaatgccg tcccgtcatc ggatacgtaa aggttcagcg tagcgaagcc
    19201 gagaaagggg acgataggca tccccgaacc ggttgagatg gttttgccag tagcgtcttt
    19261 aacaacgata ccccactttt cattgccggc gacagtgtcc cattccctca caccatcgac
    19321 ggctttcaga gaaacgtcgg tgatcgctcc tatacctttg atcgagaccg aaagatgcac
    19381 gtcgggtttg ccgtccggtt ctgatgattc ctccattcca aggagatcct cctgtgcgga
    19441 tcgaaaggag aattcggtta tctcacccat tgccccccat gccgtgcccg atacgagcaa
    19501 gggcaagagg aaagtcaaaa acagtgtcct gaaaaaacgg gttttcatga tgctctccct
    19561 ccgtctggtt tctgaaataa agatttactt gattaatcag attcagtata acatgaagaa
    19621 gtggcggtac cgatggttac cgtccatccg aagcggcctt gaggatctcc ctgcagaaag
    19681 agggaaggtc cgacggtctt ctggaggtaa ttatcctttc gtcgctgatc acttccctgt
    19741 cgatccattt tgctcccgca aggatcatgt cgtccttcag gctgggataa cttgtgactt
    19801 tctttccgga gagaatgccg caggaaacaa gaagacttcc cgcatgacat attcccgcaa
    19861 cgaatcctcc ggaggaatat gtccttttta cgagaccgat gatgtcaggt gacattctga
    19921 gtctgtcggg ggcccagcct cctggaacca cgatcccaag cagcgtttcg cctgtgatgc
    19981 ctcccgcatc gtaaggagcc tctacggtgg taccgtattt tcccttgtag actgtcggag
    20041 ctgccggtgc aactacaatg gattcgtatc cggcctcctg aagccggtag aaggggtacc
    20101 agaactcgat gtcgtggaaa tcgttttcga ccagaaccgc gatctttgaa gtcattcccg
    20161 aaccctcctt tcctgaggac agatcttttt gtcttcccca ttatagaata actccctgat
    20221 gccggggcaa acactccagg gggaaatggt gtaaaatatg tttcaagaag tctccttttc
    20281 gggggtcttc cagatcgaat cgccgcggcg gtccttccgc cgctttttgt tgttattgca
    20341 ggataaggag aggaagcgca aatggagaag aaatcgggag aactctcttt cggcaaggtc
    20401 attccgttat cgatagagga agagatccgg cacagttatc ttgactatgc aatgagcgtc
    20461 attatcggcc gtgcactgcc ggatgccagg gacgggctaa agcctgtcca gcggagggtc
    20521 ctctatgcca tgctggaact ggggctgagg cacaaccagt cctacaagaa atcggcgcga
    20581 gtcgtcggtg aaaccatggg taagtaccat cctcacggag attcagccat atacgacacc
    20641 atggcccgta tggcccagga cttctccatg aggcagaccc tcgtggatgg ccagggtaac
    20701 ttcggatccc tcgacgggga tccccctgca gcgatgcgat attgcgtcac gggagatacg
    20761 cttataaggt ccggggacag ttttcggaag atagggaata tctgtgaaac cgaagataac
    20821 actaccgttg agacagcggg agaatgcctg acccttgaca tgttgcccgg tcgttatgac
    20881 agggttttca attgtggtgt ccaccctgta attgaacttc tgacggagtc cggtttcaga
    20941 gtggagggga cctccaatca tcctctcctc atgatccagg cagggaatga aggtcgcccc
    21001 gaacttgcgt ggtcgaccat ggatgctatc aatgaaggcg attacgttgc catggcatgt
    21061 gaggaaggga attttcccga cgaacttgaa gataaatcca gggatcgggc aatattctgg
    21121 ggcggtatga tctcggaagg ctatatttcc ggggatgccc agagatataa ccgtgtgggt
    21181 ttttccaaca cagaccctga atattatgat gccgtgaaga gaggttttct tgccgagatg
    21241 gaacataccg gcaatgaaaa tggtaacctg agagaatctc tcggccacaa gacaaagaaa
    21301 gacgtaatgg ttctggatat tgattacaag cctttccggg aagcaatgat cggatccggt
    21361 tatgcatgca gggcgcggga aaaatcggtt cctgatttcg ttcttgaggg atccgcctct
    21421 ttccagatat ggtttctcag aagcctcttc gaaggagatg gcagctgctc caggcttaac
    21481 gacaaaagga gaggcaaagc atatggcgcc gtagcactgc attcggcaag cctggaacta
    21541 ctgaggcagg tccaggtcat gcttttgagg ttcgggatcc tggggcgaat ataccgcgac
    21601 aagggtatcc acaggctcct agtgacatca tatccggatc ttgcgcgttt tcgggatgtc
    21661 ataggttttc ttacggaggt caagaactcc cctctccgtg aaatttgcga agaaatgaag
    21721 gacaggagcc atttcggtcc cggatctcgc ctgaagtgta ttccctatct cgcccgatgg
    21781 atcagggaaa tagcggtaaa aaacagggag aggcttcaga aggttaatct ccaggatctc
    21841 gcaggtatca gaaagaactg ggatctgatc caggcatctg ttccggagac ggatttttcg
    21901 tatcttgtgg ggatgctcag acattctttc atattccaga aggtttcttc tgtaagatcg
    21961 tcaggccgca aaagggtttt ttcgatacgc gttgattctg cctgtcacag ttttgtgggt
    22021 aacggtttta ttaaccacaa cactgaagcg cgactgtcgt ccgcgggaga acagctcctt
    22081 tccgatatcg acgaggaaac cgtcaactgg gggccgaatt tcgacgattc acttcaggaa
    22141 ccacttacgc ttcccacaag gatcccgaat cttcttgtca acggaagcac tggcatagcc
    22201 gtaggcatgg ctaccaacat gcctccccac aacctcggcg aggttgtgga tgctctctgc
    22261 cttctcctgg ataatcccga tgccggaacc gaagaggtta tgtctgtcct cccggggccc
    22321 gatttcccga cgggcggtat catcctggga aggggaggga tactcgacgc gtaccgcacc
    22381 ggcagaggca agatcatagt caggggaaga gcccatgtcg aagagggccg gaggggaaaa
    22441 gattccatag tgataaccga gatacccttc atggtcaaca aatccaacct cctcgaggcg
    22501 atagcaaagg gggtccagat gaagaccctc gatggtatca ccgatctgag ggacgaatcc
    22561 gacaggagag ggctcagggt cgttctcgaa cttgcaaggg atgcaaaccc cgatgtgact
    22621 ttgcggcagc tttaccgcag gacacagtta cagtccacct tcggtgtcat taacctggct
    22681 ctggtcgatg gagaaccgaa ggaactctct cttctttcga tgcttggact cttcatagat
    22741 tacaggcgcg aggtggttcg aagaagaacc gtattccgcc ttaggaaggc cgaggagcgt
    22801 gctcatatag tcgaggggct tataagggcc atcgatatga tcgatgccgt gataaaactg
    22861 atccgaggtt caaaggatcc ctcggaagca cggtcgggcc ttgtcgaaaa acttggtttt
    22921 accgaggccc aggcccaggc aatactggac atgaggctcc agcgccttac gaacctggaa
    22981 aaaaacaagc tcgaggacga aatggcactg cttcttgccg acatagaaag attcagaagc
    23041 atactcgcga atccggcaac tcttgacatg gtcgtcagag atgaactccg ggaggttcgc
    23101 tcgaatttct ccgatgacag gagaaccacc atcgtggaag ctgtggacga cgtctccgtc
    23161 gaggacctga tacccgaaga ggagatagtg gttgtactct cccgtgacgg ttatcttcgc
    23221 aggatgcccc ttgagcagta ccggctgcag gggcgcggag gaaagggggt caagggagct
    23281 accccgaaaa taggagacga ggccgccata ataacggtct cgaccaccca cagggatatt
    23341 ttccttttca ccaccagggg aagggttttc gccgtcaggg ggcacgtgat ccccgaaccc
    23401 aggactggca aggggcgcct ggcaagccag tttgtatccc tggaggacgg tgaatccgtc
    23461 gtagggatga gggacagcca tcttcacgga gcccggttcg tctttttcat aaccgcggca
    23521 ggagttgcaa agcggcttcc cgtcgaggag cttgaaaatc tcacccgtgc cgggaggagg
    23581 gttctctccg tcgatgaagg tgacgagata tcccgggtca ggttttccag cggggtcgat
    23641 gatatcctca tagttactgc aaagggacag gcactgaggg tctccgaggg cgagttccgt
    23701 cctatgggcc gccaggcgag gggagtgagg gggatccgcc tttcagcaga tgacagggtg
    23761 gtaggatgcg atatagtaaa acccggacac caggttctcc ttgtcagtgc ccagggtgtc
    23821 ggcaagagaa cacggtacga tgagttcatg ccccatcaca gggcaagcgg gggatgccgg
    23881 gccatgaagg tcaccgacaa gacgggggac ctaatcgggt cctgggggct gatgcctgac
    23941 gaccaggtca tagtgatgac ccacaaggga agagttataa ggctgggtgt gggtgacatt
    24001 tcgctcctgg gcaggaccgc tacggggtac cgcgtgataa gggttgccgc gggagactgt
    24061 gtggctgaca tcagcgtcat aaggacaagt gaggaagagg ttgagtaggc tttgaggatc
    24121 gcgcctgaag gaagaatgca actgcttctg tcgatattgg cggcaatttt gtccgcggtg
    24181 tttctgccgt tgccattcgc gatggttttc ctgctgctgg cggctttcct tgcgtggttt
    24241 ttcagggatc ctgagagata tgcccgctgc ggcgacgggg actgggtcag ccccgcagac
    24301 ggaaaagtcg tagagatata tccttttgaa catcatttta cgggtaaatg taccgtcgta
    24361 ggtgttttca tgtctcctct cgatgttcac gtcaaccgta tgccctgtga cgggacaata
    24421 tcgtggctgg aatatattcc gggaaagaaa ctgatggcct tcaacccgaa ggcgtcgctg
    24481 gagaacgagc gttttctcat gggtttcgat accgcccacg gcagggggat gacggttcag
    24541 gtagcgggct ttctggcgag aagaatagta tgttccaggt cggccgggga aaggctcctt
    24601 cgcggggaac gcttcggcat gataaaattg ggatcaaaag ttgatctgta ccttccggaa
    24661 ggcgttgtcc cggcggtgaa agtcgggcag aaagtcaggg cgggagagac agcgatcgga
    24721 gtgatgcaaa atgagaagaa gaaaaaccga taaaccgata ccactgagag tgataatacc
    24781 caacatgata acaagcggca acatgctttg cggcatgctc tcccttgtcc ttgccttcca
    24841 cgggcatttt gttccggcat cctggctcat ctatatcgcg gtgatcttcg atttcatgga
    24901 tggcaagatg gccaggaggc tcggaggcgg aagcagtttc ggcatggaac tggacagcct
    24961 ggccgacgtg gtgagtttcg gtgtcgcccc ggcaatgatc atgtactcgg tctacctgaa
    25021 aggctttttc ggggttaccg gtgccgtggt agcagccttt ttcgcgcttt gcggagcctt
    25081 gagactggcc aggttcaacg tgcagcatgt tgacggctcg ttccagggat taccgatacc
    25141 ggcgggggga cattttcttg tctcctttgt tgtcgccggc atctatcttc atccggcgat
    25201 aatggcgatc gtggttgccg cggcaggagt gctcatgata tccagcgttc ccttcggtaa
    25261 cctgaaggcc ctcaaacctg gcaacctgga caggaaaaag gcctttttcc tctgggcttt
    25321 cgccttgacc ctgatagttg ttctcaggaa cagggcaccc ctgacaggaa tacttattta
    25381 catagcgagc gggttcgccg gtgtcgactg gggcagatgg ctctccctgg agaacgttag
    25441 cgaagaagat cttgcggatg aagaggttta gagcataaat cagcgtctgg tctggaggtg
    25501 cctttttccg tgaacagcct gttggggtat ctgatagtgg gagctatagc tggatggctt
    25561 gccggtaagg tctggcaggg cagagggttc ggttttttcg gcaacctggg cgtgggcatc
    25621 cttggagcct ttgtgggtgg ggttatcttc agttccatcg ggctctatcc gatgggtttc
    25681 attggttcac tggtcatggc ctttgccggg gcggtggtcc ttcttttcct ggtgggtctg
    25741 ctcaggggca ggagaaggta gcaaagagtt tccggcaagt aaaaaacagg ctcccagggg
    25801 aggtccttgc agggcagccc ctgggagctt tctgtcaata ggccttgccg agccttggcc
    25861 agatgaggta tatcgccaca accgacagaa gcagcgaggg aagcatgaaa gaaccgttgt
    25921 aaatggcgga gtagagccag acattggttc cttccggcgc ataggccgcg aaaaagatta
    25981 ccccggacag aaggtggcaa atgaaacggg cgctggaccc gatgacaggc ccgagccaga
    26041 gcgggcgtct gatcagggct gccagggaaa gtgccccgaa ggccagcgga taatcgagaa
    26101 gggcctgtgc cggatggacc acgtaccctc ccaggatcaa ctgtagaaga cctgccacgc
    26161 ttcctgcccc gaaaccggcc cggagccccc accgtaaaga aaaaacaagc agcgggatgt
    26221 tttccagggt tattgaacct ccctgcggca ttcgccaaag acgaacatag gagagagcca
    26281 gtgccattga tgcggcaagg gctccttcaa caagtatcct tacctttctg ttttccattt
    26341 tcccgtcccc tttcggtatt tagaaacggg aacctgtcct ggatcgaaga aaaagccccc
    26401 cggatttccg gggggctaac ggggccaatc tggatatctg tcccgcaaat cccttcgccg
    26461 gcatgacccg gatcaggttc caggggtcga gaccttaagt ctcctctcag ccgtcatcag
    26521 acgactcccc cattgcgatg ttctttatac gtcggcaagt tgtatttgtc aaaccctttt
    26581 attgttctgt tattgttctt tgctgccggg tgatgttata gtcgggtgca gatacaatga
    26641 agcacaatca taatttcaga ggtgatactg ttgctgaagt tcttctgccc caggtgtggg
    26701 aaggtctgtg aggaagcgtt aaggcccgtc agatgtcagt gcggttcgcc gctgaaaagt
    26761 tcactggact ggaaaaacct tgacatcgac gcttctgctc ccggcatgtg gagatattct
    26821 ggtgttcttc cccccgtgga cagatccgct tccatgggag agggttggac tccaatgatc
    26881 agtgggggca gcagaaacct tttttataaa ctggagtatg tctctcctac cgggtctttc
    26941 aaggacaggg gggctgcgat agtcatggga gaggcgaagt ccctgggctg cggctctgtg
    27001 gtggaggact catccggaaa cgccggggcg tcggtcgccg cctatgcttc cagaagcgga
    27061 ataaaggcaa ccattgtgtt tcccagaaat gtatcggaac agaagaaaag acagatagaa
    27121 ctttacgggg cggaacttgt ggaggccgaa gggggcagaa aggctgctgc tgaaatggcc
    27181 atggagatgg ccaggtcaac tttctacgcc agtcatgtct ggaaccccct cttccttgaa
    27241 gggaccagaa ccatagcctt tgagatatgg gaacagcttg ggaaaagggc gccggaaaca
    27301 gttattgttc ccgcaggcaa cgggaccatt ctcctgggga ttttcaaggg attcaaggaa
    27361 cttctccaga gggtcaagat caatcgcctt cccaaaataa tcgctgtcca gagcgaaaac
    27421 tgctgtcccc tctttgacgc gtggaagggg acatccactg aaccatatcg aaatactgtg
    27481 tctgagggga tagccgtggg cgacccaccc cgaatgaagg aaatgataga tgccgtaagg
    27541 ataagcaggg gagatgtagt aaccgtagga gacgaggccg tgctcgcaga aaggaaaagg
    27601 cttgctgtgg agggcggtct gttcgtcgag ccctcggctg cggttgcgcc ggcggcggag
    27661 cggatgctca gggaaagcgg aaaaattact gaaaaagaga ccgtagtggt ccccctgacc
    27721 ggaacggggc tcaagggaac atataaacag tgaatcgaat gattcaaagg cgtgaacggt
    27781 taacggtgtg caaaggcaag tgcgtgaggg gttagacgtg agaagtgaga cgaaaaatac
    27841 gggccaagga ctccttggtt ttccaaaaga agggttttct ctgtgtggat ttaccctctg
    27901 tgtcccctgt ggtgagaagg gccgggggat cgttttatga gagtgcgtag tgaag
//
LOCUS       DAOTIQ010000017        27695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_1974_length_27695_cov_6.928980, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000017 DAOTIQ010000000
VERSION     DAOTIQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 27695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27695
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_27695_cov_6.928980"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="P5541_03825"
     CDS             complement(<1..673)
                     /locus_tag="P5541_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HRV71139.1"
                     /translation="MSLSPSMSSGFTAARNRSKYVSPLSGMCSLCTEECPGPCEIAQA
                     AVLGKITVYPTTTGPNQIASEKDYPVDFSHFNINGRCFGAMGTEPDHEHAEIFNVDLA
                     SEYGCDNRVKLDLPIVLPALVKMNWVDYFGGAAMAGVTCMVGEDAREKDAELKFENGK
                     ITSFPMLKKIYDSFHKYYRGSGQMVLQCNVDDHLAGVPEIALKKYGFEALEFKFGQGA
                     KGTQPA"
     gene            1007..2650
                     /gene="hcp"
                     /locus_tag="P5541_03830"
                     /gene_synonym="priS"
     CDS             1007..2650
                     /gene="hcp"
                     /locus_tag="P5541_03830"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRV71140.1"
                     /translation="MYCFQCQETAKNQGCTVRGVCGKAEETADLQDLLIYVCKGISVF
                     GEELKKRGVVDREAGRFICKALFVTITNAAWDNNAIIDWIREGIRVRGEVAKKAGDVE
                     GPLPDCATWRAESNEEIIAKAMSDEVRITATGNEDIRSLRELLVIGSKGIAAYAEHAE
                     VLGFEKDEIYAFLMEGLASTTRDLPTDEMVGMVMKAGEMAVNTMALLDEANTTTYGNP
                     EITEVYLGVRNNPGILVSGHDLKDMEELLEQTSGTGVDVYTHGEMLPANYYPAFKKYG
                     HFVGNYGGSWWHQNEEFESFNGPILLTTNCLIPVKNENTYLDRLFTTGVVSYPGARSI
                     PARPKNGKKDFSGIIEMAKKCATPKEIEKGKIVGGFAHNQVMALADKVVEAVKSGAIR
                     RFVVMAGCDGRFKSRDYFTEVARNLPKDTVILTAGCAKYRYNKLALGDIGGIPRVLDA
                     GQCNDSYSLAVIALKLKEAFGLADVNELPISYDIAWYEQKAVAVLLALLSLGVKGIRL
                     GPTLPAFLSPNVAKVLVDKFDIKPTGTVEEDIAAMMAGK"
     gene            2945..3973
                     /locus_tag="P5541_03835"
     CDS             2945..3973
                     /locus_tag="P5541_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV71141.1"
                     /translation="MKRLLCTLITAVLVLGALGGSVAASPIEKEVVFGDQSWDSIQFL
                     HRVMGYILQHGYGMSPVYSFSESMPSLIGLERGDNHIILEVWVEARQEWWDSARQAGK
                     VLGFGKVFPNAPSGLYVPSYLVKGDPERGIEPLAPDLVSVFDLPRYQHLFPDPEDKAR
                     GRVYNAVSGWAANERYIKKFDGYKVEGKPLREYFNLFDPGSQTALNAAIRGAHDRGLP
                     IIAYYWEPTPLLGELDMILLKEPEYDEKIFRETFLCQNPAFSVEKAANAKWVSEHPEV
                     VSLLEKFHLSLEETNEVLAWMEAHENDPQKAVLWFLKNNADLWHSWVDDPKVKEMLEK
                     ALEKEKIQ"
     gene            complement(4092..5462)
                     /locus_tag="P5541_03840"
     CDS             complement(4092..5462)
                     /locus_tag="P5541_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV71142.1"
                     /translation="MSYDLRTEVMGRDPVGSLLFRFSMPAIVGLLSNALYNIVDRMFI
                     GRIVGSRGLAAVTVAFPYFGLAITAGILVAVGSSSLVSRSLGRGDRTRAEEVLGNAFL
                     MAIAGGSILLLAGYCCGEQMLTLFGASKLVIREALGYLRIVALGMPFLVITFSMNGLM
                     RSEGAPRWAMGTMLVGTMTNIFLDWLFIARLGWGVKGAAWGTTIAQAIALGWVLYFYR
                     YYSNLKIRRPLLLRPKKEIMLSALAIGVSPGLLEISFVILLILINRIMGGLGGDLAIS
                     AVGIFLSLDSLFFLPALGIGEGAQPLIGFNYGAGKMDRVKSIVMMAIAAAAGIFSLFW
                     VIVQIFPVFLVGLFSRGDADLSAMTVRGLRLGYMLLPLMSLYIVTSYTFQALGKARAT
                     FVLQVLRQIVFCFPMLLILPRILGIDGVWLSFPVMDLSGFLLALLMLRMEMKRWKIPG
                     ENDETA"
     gene            complement(5554..6132)
                     /locus_tag="P5541_03845"
     CDS             complement(5554..6132)
                     /locus_tag="P5541_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRV71143.1"
                     /translation="MENILALFVGLGLSASCGFRVFVPLLVMSLAAQTGHIELAEGFQ
                     WVATPTATVALGTATFFEIAGYYVPWFDNMLDTVATPAAIIAGTFITASVLPEMTPFL
                     KWSISAITGGTAAGLVQGATVLTRAASAFTTGGLGNPVVSTAESGGSIFLSLMAILLP
                     LAAMALVLFIFAWAGRKIYRRSVKGKATSGQA"
     gene            6303..7184
                     /locus_tag="P5541_03850"
     CDS             6303..7184
                     /locus_tag="P5541_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV71144.1"
                     /translation="MHDIEAIPSPGRKYRKRDLEGLDPVLLGALLRERIHHNIEVPLY
                     SMLQRRPFKAVPNFGLQAQLVYDEWVRRGLPQDGPDLDWAKRYIDIAAQVRRGERPGI
                     PELVVSIPEPFTKSEMEVVRKLIWGRCSIRNWTSRPVPEDLIEKILEAGRAAPVGCNL
                     DAVRFVVLRTPEEKSIIWSDIPTDNAVIIVICYDRRIAETVGQDKMVPQNLGYDAAAA
                     GDHMLLMAHALGLGGVWLSKTAKSEHTEDTGKIFKELYGLPDHIEVALHVAVGWPASG
                     IIKSKRIPLSEMIIAKK"
     gene            complement(7375..8124)
                     /locus_tag="P5541_03855"
     CDS             complement(7375..8124)
                     /locus_tag="P5541_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRV71145.1"
                     /translation="MGTERGWPPIPQGKPFDWEEAQSVPIDECREELVPASLVPERLL
                     SHPVYFLNGLDGAIAECYVRRRVLSLLLEAARLLPSGLRLVLLDGWRPPSLQSLLFKR
                     YCAELREIMPDKDGDYIARMASRFVAAPSTDPGRPSPHVTGGAVDITLADKDGFLLDM
                     GSAFDETTDRSGTVYYENKVLSGGATSENTLIVLENRRILYKAMTGAGFTNLPDEWWH
                     YDYANQNWAFMKGEKAAFYGVCEPPFRWRDR"
     gene            complement(8138..9427)
                     /locus_tag="P5541_03860"
     CDS             complement(8138..9427)
                     /locus_tag="P5541_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV71146.1"
                     /translation="MGFLMLFSFLVLMVLGMPLYLSLLSASLAGILALGDLSLLRVMS
                     QQFFGGMDSFTLMAIPFFILAGILMNRSGLTDRLLDFCRLLVGSVHGGLGYVNVVGSI
                     ILSGVNGSAAADASALGSILIPAMVKDGFSPSYSAGLTAGSALIGPIIPPSIFMIIYA
                     TMTNTSIGGLFAAGILPGLVLGGAFMVMNYFYSIIYHVPVTDLEDVRKRKKEILARSA
                     VALLAPLIILGGIITGIVTPTESGALAVAYCLVTGVFYTRQLTWRTLGESLYETVRLT
                     SSIFLIMGAATVVGWLLKWEQIPQRFAAFILEAGLMDRPWLLLVVLSAVIFPVGMFME
                     EISTLTLLTPIFAPIALKAGIDPLQFGVVMTLNVTIALITPPMGACNYIVAAVGKVRL
                     TDVFVHIWPFIGVALVVLMAVITFPFLTTLIPWILNL"
     gene            complement(9429..9923)
                     /locus_tag="P5541_03865"
     CDS             complement(9429..9923)
                     /locus_tag="P5541_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV71147.1"
                     /translation="MMFSGSQGEKGLERWITRLETLSAWTACFMLAVNVGDILLGVFC
                     RYVLKSSLIWTEEVARFSLVWIVMLGALGAAVRGDHMTVDFVVPRFPQYLQRAVELGR
                     FLLSVAILILMICLGWVNATRIWHMKTLALNIPKTFPLMALPAGFALLLAGTVLLHLR
                     GRES"
     gene            complement(9931..10968)
                     /gene="dctP"
                     /locus_tag="P5541_03870"
     CDS             complement(9931..10968)
                     /gene="dctP"
                     /locus_tag="P5541_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV71148.1"
                     /translation="MRYGKFFAAILLLVFCLAVSGTSMASVEVKMSYNGPADVDNNAV
                     HLYAVNFKKLVEEGTGGEVTIRLFPDSQLGNEEERMELLTKQGMNQPVINVASFAGVA
                     PVFPEIYASAVPFMFDSYEAAHLFFDESRYWNRAREEFHQRTGAYLLEAVEEGGFLAF
                     TNNKKEIRTADDFKGLRFRGMDEGQVILFESFGASGTPIPWTELYMALKSGVVDGQMN
                     PAMYIKMGSLYEVQKYLTLANIQYSDQFLVINGDLLDSLPAEQRSVIIEAAKKANALN
                     RQAMQDADQKDIDFLVEKGMIAYAPNHDEMETLRKAGQPAYVEWLKAKVGQDWLDLAL
                     ECARNANEKSK"
     gene            11164..11928
                     /locus_tag="P5541_03875"
     CDS             11164..11928
                     /locus_tag="P5541_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRV71149.1"
                     /translation="MTKDVERKKVGLALGGGAVLGAAHIGVLKALKENNIRVDLVSGT
                     SVGSFIGALYAFGMPWDQIDRITRELKWLDVSSISISRYGLLSNSGLGELIMEHLGDV
                     TFEQAKVPLAVVATDISSGEEVVLTSGSVAQAVMASSCIPGVFVPVEIDGRLLVDGGV
                     VENVPIDPLKRMGAEYVIGVDLNSGRRAEKPENIVDVLLRSFHFLRRSATRRQTGEAD
                     LLIVPDLTGFNMYDLDQAGELFEAGYQEAAKMLGDL"
     gene            12089..12250
                     /locus_tag="P5541_03880"
     CDS             12089..12250
                     /locus_tag="P5541_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71150.1"
                     /translation="MKKFLTAMLIVLMISGVAFAGGDQNCEQHHSDKGQGSVGASNPG
                     NGDQTQKGK"
     gene            12420..13271
                     /gene="panB"
                     /locus_tag="P5541_03885"
     CDS             12420..13271
                     /gene="panB"
                     /locus_tag="P5541_03885"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRV71151.1"
                     /translation="MAKVTIQQLQKMKEDGEKISMVTAYDFSQAVLVEKAEIEVILVG
                     DSLAMTTLGHEGTTALTTDEMIAHIRPVVKGAPSPMVIGDLVFGSYNESIAQAIHSSN
                     RLIKEGGCDAVKLEGALPDTVRAIVDAGIAVQGHLGLTPQTAVLLGGFKVQGKGHDAA
                     LKLLDDARRLEEAGVFSIVLECVPEELGRVMSQIVSVPVIGIGAGRFCDGQVLVFHDM
                     LGLFDRFTPRFVKQYRQIGQEIVGAFQEFKEEIRNGSFPSEEHAFSGFSREEADGILA
                     GIKSRMN"
     gene            complement(13268..13783)
                     /locus_tag="P5541_03890"
     CDS             complement(13268..13783)
                     /locus_tag="P5541_03890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRV71152.1"
                     /translation="MIEIREERPEDIQGIRNVNELAFGQPMEADIVDRLRENCPDRLS
                     LVALEAGRVVGHIFFSPVTIEVSGRVIEGMGLAPMAVLSDRQGQGIGSHLVRKGLEVL
                     RERNCPFVVVLGHPSYYPRFGFEMASKYGIACQWENVPDEAFMVLILNWVAMEGARGV
                     ARYREEFDEAV"
     gene            13932..14231
                     /locus_tag="P5541_03895"
     CDS             13932..14231
                     /locus_tag="P5541_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71153.1"
                     /translation="MGSCKEGDQKATRKREPLAVQEGIRQGALTERSSAREGIERQLF
                     PWPGCCPFPEIPDHGAEDATGEEGDLETVGTSCETCSGRKTPVVARPTAIRPAPQ"
     gene            complement(14291..14848)
                     /locus_tag="P5541_03900"
     CDS             complement(14291..14848)
                     /locus_tag="P5541_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRV71154.1"
                     /translation="MDKKEKKLVPFDEAREQIERVCRRLAMLHIAYARTLADEFGVEE
                     GKRLALRAIKDYGKRIGEDSLKRTLAKGLDNMPENYSEDLPTYGLYENLERLETEKGK
                     TMRLKGCTMGKTWKEMGEEELGRIYCYVDPAKSMAFNPDWALVHSRCMPDGDEFCDLH
                     YRKTSKKEKEEFLEKETDLSKIDRI"
     gene            complement(14909..16111)
                     /locus_tag="P5541_03905"
     CDS             complement(14909..16111)
                     /locus_tag="P5541_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRV71155.1"
                     /translation="MEFSKRSKALPASPIRKLEPFAAGAKAKGKKIYPLHIGQPDIET
                     PTLFFESLKSFDNKTLAYGTSQGDLALIDKILEYYAKIGIEFERGDVLITNGGSEALN
                     FAINVACDPGDSILVPEPFFPAYAAFAVSASARITPITTRAEDGFHLPSREEIEKLID
                     TSTRAILLSHPGNPTGVVYTGEEVRMLSDVAMKHNLLLVADEVYREFVYMDEPFLSFG
                     SIESIRNHLIIVDSVSKRYSACGARIGCLLSRNREFMDLGLKYCQARGCSPTLEQLGA
                     AALYETPDSYLAEVNEEYRKRRDTLFEGLNEIPGIVCRKPEGAFYIMAKLPVDNAEKF
                     IIWMLNEFSDDGETVMAAPAEGFYATPGLGGQEIRMAYVLKEKDLKRAVELLAKGISQ
                     YPGRRSGF"
     gene            complement(16197..16523)
                     /locus_tag="P5541_03910"
     CDS             complement(16197..16523)
                     /locus_tag="P5541_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRV71156.1"
                     /translation="MDERLENVTVGTKANVYFDGKVVSRNVFFPDGTRKTLGVILPGT
                     FEFEVGDKEIVKIVSGKAEVLLPPDKKEWITVEKDDSFTVVRNSSYQIRCCEIVEYIC
                     DYIPEA"
     gene            complement(16555..17862)
                     /locus_tag="P5541_03915"
     CDS             complement(16555..17862)
                     /locus_tag="P5541_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV71157.1"
                     /translation="MENVPLLMFLVLFIALAMGIPIAFALTLVALVFGWSLWGVGGMK
                     LLVTAAWGTMNNFALLAVPLFIFMALILQKSRVVEDIYDSFYKWSGPLRGGLAVATIL
                     VGAILGAVSGVVAAGVIGLGLIGLPQMLKYGYDQRISLGSVMAGGTLGQLIPPSTNMV
                     IYGAVTGVSVGSLFAGGLTCGTFLALLYCGYILTCSLFNPKVCPALPLEDRVDFRGKI
                     RALRKVFLPSLLILLVLGSVLSGAATPTEAAAVGAVGALFFSAVTRRLNWGIIGSASL
                     ETLEISSMVGWIVIAASAFGAVFSGTGGNRLVTEIAMSLPGGPWMVFLASVIFVFFLG
                     MFLEPTAMIMLAAPIVSPILSKLGFDPLWWGLVFMVLLQVAYLSPPFGFSLFYMRGAA
                     PDHIGIEEIYMSSLPFIGLQFLSLVFLVFFPSIALWLPRLLLS"
     gene            complement(17867..18337)
                     /locus_tag="P5541_03920"
     CDS             complement(17867..18337)
                     /locus_tag="P5541_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV71158.1"
                     /translation="MERLGRYTSYIILPLAFIIVVAVIMRYVFNNMPGWAFEISLFLY
                     GVHFMLGGGYTHLKGKHVAVDIIHKYLSPKNKRRMDFLSEMVVAFCSAVLVYISTGWA
                     IQSIKIMERSMHQTTFDPPIWWFKCIVPISALLLLIASIKNIIRLSRGSLPESR"
     gene            complement(18423..19496)
                     /locus_tag="P5541_03925"
     CDS             complement(18423..19496)
                     /locus_tag="P5541_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV71159.1"
                     /translation="MRRFSILVAMVLVFAFCFTAIGDFSKAEAEEKVYKWRLVTHSMV
                     GTYRYKHVEAFADMVNKASNGRLIIEPYGAGVLFPVFDSFDAIRDGVVEMGMIYSAYW
                     AGKDPFFAIFATRPGSPILTFHEGMYLDQRAFPIAEKVYKKHGITYLGTVDCSVPEIL
                     MMSTPIRSLEDFKGKQIRSSGMGAQFYTALGASAVSLSAPEIYTAMQTGTIDAAEWTE
                     WKENEEMGLLEVTKFVMDPAHHGGTNEDKGLIVNEKKWAELPDDLKAIVLAARDHMRY
                     LSAVTNYSEAAKTKQQWLAKGVDIITLPQEDVVKMREIAANVLNEWGKKNAECQEYLE
                     LYAEVLNELGYTKEAATLGYKAK"
     gene            19826..20377
                     /locus_tag="P5541_03930"
     CDS             19826..20377
                     /locus_tag="P5541_03930"
                     /inference="COORDINATES: protein motif:HMM:NF006321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HRV71160.1"
                     /translation="MLLVELRIHGRGGQGSVAMAEMIAGAAFAEGKFAQAFPYLGGGG
                     ERRGAPVQAYARISDSPIKLREKIEEPDLVIVQDPTIMEMVDVLSGLKKDGVILVNSE
                     SSMELSRDDVRAFFVPASRIAIETIGRPIMNTALIGALARITGLVSVDAVAEAIREKF
                     PGEIAEKNVLAVRKAYEQVGGVE"
     gene            20377..20634
                     /locus_tag="P5541_03935"
     CDS             20377..20634
                     /locus_tag="P5541_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71161.1"
                     /translation="MDLPLGGVCKAGESKKLETGKWRSFRPVVDKAKCIKCGRCAEFC
                     PDSCMEIKEDGAEVNLYYCKGCLICMRECPVDAISKEVEEK"
     gene            20631..21821
                     /locus_tag="P5541_03940"
     CDS             20631..21821
                     /locus_tag="P5541_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV71162.1"
                     /translation="MKGKTAILEGSRAVAEAVRACRPDVISVYPITPQTHILEDLARF
                     VSSGKLKSQFVRADSEFSAASIVYGASAAGARAYTASASQGLLLMNEVIYAMASTRLP
                     VVLTAVNRAISAPITIQPDHQDTMSLRDTGAIQIHVESMQEAYDAHVQSFRIAEDPEV
                     QLPLMVCMDGWVISHSYEPVTVYPDEAVAEFAGSFTPVQKLDPSNPLTYGAYADQDVL
                     MEYRYAMMVAQEKARDKIRSVTKEYREVFGNFDGDLVDTHCTEDAEVILVAMGSMVST
                     IRAAVDELRSEGRKVGLIKVRTFRPFPREEIIAACGKAPVVAVLDKSLSVNSGGILAT
                     EVKSAFYGENVQPVIVPFMAGLGGKEVNRRVVKEIVDIASRAAEKQGAQETVWMGLDR
                     SFFS"
     gene            21847..22749
                     /locus_tag="P5541_03945"
     CDS             21847..22749
                     /locus_tag="P5541_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV71163.1"
                     /translation="MTIQTEVKELHAPGHRACPGCGCAIAVKLILRETGPNTIVVSPT
                     GCLETFTSPFRGSSWEVPWIHSLFENAPAIATGVLAGLNSKGNVDGTKIVAIGGDGGT
                     YDIGMGSLSGMFERKDDILYICYDNEAYMNTGIQGSSATPLGAGTTTTPVVGNSFGKM
                     VSKKDLLAIALAHDVPYAATASIADPADLKKKVRKGMDNPGPAFILMLTPCNLGWGFE
                     PENTVELARKAISTGLFPIVEFENGRLVSVQKINPVPVEEYLKPQKRYQHLYKRDDFN
                     EVLKAIQEKADRNIEKYDLAKDRN"
     gene            22780..23637
                     /locus_tag="P5541_03950"
     CDS             22780..23637
                     /locus_tag="P5541_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRV71164.1"
                     /translation="MKKVMVYGTGTMGTGIAQVLASSGVEVLLVSARGGTENSSRGKG
                     KVGSILDRAVQKGKETAENAKVIMDRIHPVADMAEGISGVDMLIEAATEEMELKKEVF
                     RKAESLDPGEKIIFATNSSALSITELATVTKRTDRFLGVHFFNPAPVMALVEVVKGAA
                     TDEDVVAEVVTFIESLGKSPIVVSEAPGFVVNRILVPMINEAVYALMEGVASAEDIDK
                     GMKLGANHPIGPLALADLIGLDVCLSVMETLYKEFGDTRYRPCPLLRKNVRAGFLGRK
                     TKRGFFVYE"
     gene            23677..25140
                     /locus_tag="P5541_03955"
     CDS             23677..25140
                     /locus_tag="P5541_03955"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HRV71165.1"
                     /translation="MKNGQEYIESLRSLKPVVYYLGEKVDSVVDHPAFQPHVNCAALT
                     YELAMRPEYQELLSARSPFINDRVNRWTHIHENNEDLVKKVKALRFLGQKTGACFQRC
                     VGWDALNAVYSVTWEIDRDKGTDYHARFLKYLERVQRDDLMIAGAMTDPKGDRSKKPG
                     QQEDPDMYVHVVEKRSDGIVVRGAKAHMTGMVNSHEMLVMPTTGLSEDDADYAVCFSL
                     PVDAEGVIHIFGRQTNDTRRLEKSTIDTGNHRFGIVGGEALTIFDDVFVPWENVYLCG
                     EAEYAGLLVERFATLHRQNYGGCKTGVSDVIIGATAALADYNGVETASHIRDKLVEMT
                     FLAETLYSCSLACSHEGHTTPSGAYLADPMLANITKQNVTRNIYEICRLAQDIAGGLM
                     ATLPSEADLNNPEIGPFITKYLKGREGVDTETRIRMMRLIENLTGGTALVESMHGAGS
                     PQAQKIMIYRNSSIKGKKKLAEILAGVDPDPGESTRG"
     gene            25179..25967
                     /locus_tag="P5541_03960"
     CDS             25179..25967
                     /locus_tag="P5541_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV71166.1"
                     /translation="MSVTSEKEFVLVEDAAEGVLSVAINSPRTLNALNARIIGELRET
                     FRECAARSDVSSVLLTGKGKAFVAGADISELSGLTPGEAAAFSRSGYDLFQIIEEMPQ
                     PVVAVINGFALGGGLELALACDFRFVAEGAKLGLPEISLGIIPGFGGTQRLASIVGIS
                     KAREMILTGNRFDAAWSLACGLVDRVFPEDRLWDESLSFAGLLAARSSSAMSQAKKVL
                     NLSRNHPVEKGLEIENMAFAACFEHPDSHEGIKAFLEKRKPRFA"
     gene            26040..27263
                     /locus_tag="P5541_03965"
     CDS             26040..27263
                     /locus_tag="P5541_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRV71167.1"
                     /translation="MSMVSLVKVKQDDEKGIAEAVRRAVDIVGGLDDIVKKGDLVLIN
                     PNLVAVPSGRVTGAISRWEVCKAIADLVMEKGGRPVIAESSAAGVDTEKVIEAGEYTR
                     LREQGYEVIDLKPTPRAKIPVPEGHKVFSELGSWELVRKADVIISVPVMKTHDQTEVT
                     LSMKNLKGLIDDPTKKAFHREGLLEGVVDLMTALKPKLTVFDATVCQEGMGPIFGTPV
                     PMGLILASRDYVAADAVGGKIMGYDPKDVMITVRAAARGIGEMDLDRIEVRGDASIAD
                     VERRFKRASETVIEGVPPFKLMLDEGACTGCRNTVISAIMDLKSQGLQGCLEGKYVVA
                     GPLDEKLLPPCAEKDKTVLVGICAKHLEGRGTYVQGCPPNNIFIVRAIVGEGQKIERR
                     YATEEGADDQSGETK"
     gene            27320..>27695
                     /locus_tag="P5541_03970"
     CDS             27320..>27695
                     /locus_tag="P5541_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRV71168.1"
                     /translation="MREVVVIDAVRTPVGKFNGGLSAVSASDLGARVISALLERTGVE
                     ASAIDHVIMGNTLQFGENTYLSRLAAVKAGIPHEVPALTVNRLCGSGLEAINEAVRLI
                     QLGLADTCVAGGSESMTNGPFFS"
BASE COUNT         6726 a         7183 c         7696 g         6090 t
ORIGIN      
        1 tggccggctg ggttcccttg gctccctggc cgaacttgaa ttccagggcc tcgaagccgt
       61 atttcttcag ggctatctcg gggacgcccg caagatggtc gtcgacgttg cactggagca
      121 ccatctgccc tgagcccctg taatatttat ggaaactgtc gtatattttc ttcagcatcg
      181 ggaatgaagt gatcttaccg ttctcaaatt ttagttctgc gtctttttcc cttgcatctt
      241 cccctaccat gcaggttact cccgccatgg cagcaccacc aaaataatcg acccagttca
      301 tttttaccaa tgcgggcaaa acgatgggca gatccagctt gaccctgtta tcgcaaccgt
      361 attcggaagc aaggtcgacg ttaaatatct ctgcgtgttc atgatccggc tctgtcccca
      421 tggcgccaaa acaccggccg tttatgttga aatgagagaa atcaacggga taatcctttt
      481 ccgacgcgat ctggttaggg cccgtggttg taggatagac cgttatcttg cccaggacag
      541 ccgcctgggc tatctcgcag gggcccgggc attcttctgt gcataacgag cacatccccg
      601 acaggggaga aacatacttg cttctgttcc ttgccgctgt aaagcctgaa ctcatgctag
      661 gtgaaagtga cataataaaa cctcccatta aggaattgat cttaattcct gttatcacct
      721 gacctgcttg aggtcattgt atcacttgac aaaaatcgca aaaaacgatc cagcagggga
      781 ccagctctat ccttttactt ctgccctttg ttctccagat cttcatattg ccattccagg
      841 tgagttggtt ctggtaatat aatattactg tgaagcgaat tctggtttcc cctgatcaaa
      901 aagggctgaa ggtttttcga aaagtattta caggaccggg gaatagggta atatggatac
      961 cggaatacca aaatttcagg gatgatcaag ggaaggggga atggatatgt attgtttcca
     1021 atgccaggaa acggcgaaga atcagggttg cacagtaagg ggtgtctgcg gtaaagcgga
     1081 ggagacggct gatcttcagg accttttgat ctacgtatgc aaggggatat ccgtcttcgg
     1141 ggaggaattg aagaagagag gagtagtgga cagggaagcc gggcggttca tctgcaaggc
     1201 cctctttgtg accattacca acgcagcctg ggacaacaat gccataatcg actggatcag
     1261 ggaaggcata agggtcaggg gagaagttgc caaaaaggcc ggcgacgttg aagggcctct
     1321 tcccgactgt gccacctgga gggccgaaag caatgaggag atcatcgcca aggccatgtc
     1381 agacgaagtg cggataaccg cgacagggaa cgaggatatt cgctccctca gggaactcct
     1441 cgtcatcggg agcaagggca ttgccgccta cgcagaacat gccgaggtcc tcggcttcga
     1501 gaaggacgag atctacgcgt ttctcatgga aggcctggcc tcgaccacca gggaccttcc
     1561 caccgatgaa atggtaggta tggtcatgaa ggctggggaa atggccgtca acaccatggc
     1621 cctccttgac gaggccaaca ccaccaccta cggcaatccc gagatcactg aggtctacct
     1681 cggggtcagg aacaacccgg gcattctggt ctccggtcat gaccttaaag acatggagga
     1741 actgctggag cagacctcag ggacaggggt ggacgtctac actcacggag agatgcttcc
     1801 ggccaactac tatcctgcct tcaagaaata cggtcatttc gtgggcaatt acggcggatc
     1861 ctggtggcat cagaacgaag agttcgagtc cttcaacggc ccgatcctgc taaccaccaa
     1921 ctgtcttatc ccggtaaaga acgagaacac ctatcttgac aggcttttca caacaggtgt
     1981 cgtcagctat cctggtgcga gatcgatccc tgcgagaccc aaaaatggga agaaggactt
     2041 ctccgggatc atcgagatgg caaagaagtg cgctaccccg aaagagatcg aaaaaggcaa
     2101 gatagtgggc ggtttcgccc acaaccaggt catggccctg gccgacaagg tcgtcgaagc
     2161 cgtcaagtca ggagctatcc ggcgctttgt ggtgatggca ggatgcgacg gacggttcaa
     2221 gtccagggat tacttcaccg aggtggccag gaacctgcct aaggacactg tcattcttac
     2281 tgcagggtgt gccaagtacc gatacaacaa gcttgctctc ggcgatatcg gagggatccc
     2341 ccgggttctg gacgccggac agtgcaacga ctcctactcc ctggcggtca tcgccctgaa
     2401 actgaaggag gccttcgggc tggccgacgt caacgaactt cccatctcct acgacatcgc
     2461 atggtacgag cagaaagccg ttgccgttct tcttgcgctg ctttcgctgg gagtcaaggg
     2521 gatccgactg ggcccgacgc ttcccgcctt cctttcgccg aacgtggcca aggtactggt
     2581 ggacaagttc gacataaagc ctacaggaac ggtggaggag gacattgcgg caatgatggc
     2641 ggggaaatag cctccgggga tgacctgaag tgggagtggc cgaacctcca ctcccacttt
     2701 tctctttttg ccccattttg ggggccgatc ggaaaggccc tttcaagatc tctggacgga
     2761 cttgcagcgg cgacggttct tcccgtgatg accccatgtt gccggcagtg tataatattc
     2821 aagctatcct tcgtctcccg ggatacatcc ccccattctt gcctggatct ttaggagcgc
     2881 aaaaaagccg gatccctgtc tggatcctga gctggatctt aactttgaaa ggggagatct
     2941 ctggatgaaa agacttttgt gcacgttgat aaccgcagtc cttgttcttg gtgccttggg
     3001 aggatcagtg gctgcctctc ccatagagaa ggaggtagtc ttcggcgatc agtcctggga
     3061 cagcatccag tttctccata gggtgatggg ctatatcctg caacatggct acggaatgag
     3121 tccggtttac agtttttccg aatccatgcc gtcgctcata ggtctcgaac gtggagataa
     3181 ccatatcatc ctcgaggtgt gggtggaggc ccgacaggag tggtgggact ctgcccggca
     3241 ggcgggcaag gtcctgggct tcggtaaggt cttccccaac gctccttccg gtctctatgt
     3301 gccctcctac ctggtcaagg gggatcccga gagggggatc gaaccccttg cgccggacct
     3361 cgtctccgta tttgacctgc ccaggtatca gcaccttttc cccgacccag aggacaaggc
     3421 caggggtcgg gtctacaatg ccgtctccgg gtgggccgca aacgagagat acatcaagaa
     3481 attcgatggc tacaaggttg aggggaaacc actcagggag tacttcaacc tctttgatcc
     3541 gggttcacag accgctctca acgccgctat caggggtgct cacgatcgcg ggctcccgat
     3601 catcgcctac tactgggaac cgaccccgct tctgggggag ctggacatga tcctcctcaa
     3661 ggaaccggaa tacgatgaga agatcttcag ggagaccttt ctatgccaga acccggcctt
     3721 ctcagtggaa aaggccgcca atgccaagtg ggtcagcgaa caccccgaag tagtttcgct
     3781 ccttgagaaa ttccacctga gcctggagga gaccaacgag gtattggcat ggatggaagc
     3841 tcatgaaaac gatcctcaaa aagcggtcct ttggttcctc aagaacaacg ctgatctgtg
     3901 gcattcatgg gtcgatgatc ccaaagtgaa agagatgctt gagaaagctc ttgaaaagga
     3961 aaagatccag taagaggacc cggggggtca ggtcctggaa gacctttttg actgtcaagg
     4021 aaggcaacgt atcgggacag ccagcacctt acaggctgtc ccgttctcat cccagtcgaa
     4081 cagagactcc ttcaggcggt ttcatcattc tcccctggaa ttttccacct tttcatttcc
     4141 atcctgagca tcagaagagc cagcaggaat ccgctcaggt ccataacggg aaaggacagc
     4201 cataccccgt cgatccccag gatacggggc aggatcagaa gcatggggaa gcagaagact
     4261 atctgccgga ggacctggag gacaaaggtc gccctggcct ttcccagggc ctggaaggtg
     4321 tagctggtca cgatgtagag ggacatcaaa ggaagaagca tatagcccaa cctcagcccc
     4381 cgtaccgtca ttgcgctcag gtcggcatcc cccctggaaa aaaggcccac cagaaagacc
     4441 gggaatatct ggacgatcac ccagaaaaga gagaaaatgc ctgcggccgc ggctatggcc
     4501 atcatgacta tgctcttgac cctgtccatc tttcctgcgc cgtagttgaa ccctatcagg
     4561 ggctgggccc cctcgccgat acccagagcg gggaggaaga agagggagtc aaggctcagg
     4621 aagatcccca cagctgatat cgcaagatca ccgcccagac ctcccatgat cctgtttatc
     4681 aatataagga ggatcacaaa ggatatttca agaagtcccg gagatacccc gatcgccaga
     4741 gcagagagca ttatctcctt cttcggacgc aaaagaaggg gacgacggat cttcaggttg
     4801 ctgtaatagc ggtagaaata gaggacccaa ccaagggcga ttgcctgcgc aatggtggtc
     4861 ccccaggcgg ctccctttac gccccaaccc agtcgggcaa tgaagagcca gtcaaggaaa
     4921 atgttggtca tggtccctac aagcatggtt cccatggccc agcgaggtgc cccttcggat
     4981 ctcataagtc cattcatgga aaaggttatt accaggaagg gcattcccag tgcaacgatc
     5041 ctcagatatc ccagggcctc tctgatgaca agcttgctgg ctccgaaaag tgtgagcatc
     5101 tgctccccgc agcagtagcc ggccaacagg aggatggaac cacctgcgat agccataaga
     5161 aaagcgtttc ccagcacctc ttctgcccgg gtcctgtcgc ccctgcccag actccttgaa
     5221 acgagtgatg atgaacccac agctacaagt atcccggctg tgatggccag tccaaaatag
     5281 gggaaggcaa cggtgacagc cgctaggccc cgggaaccga ctatcctgcc gatgaacatc
     5341 cggtccacta tgttgtacag ggcattggac agaagaccta ctattgccgg cattgaaaaa
     5401 cggaagagca ggcttccgac gggatcccga cccatcacct ccgtacgcag atcgtaggac
     5461 acggtctcac tctccttgaa atattaactc gacaactgaa gagggaccga aggaaacccc
     5521 cggcccctct aggacagcac tttcccgggg gtgtcatgcc tgaccggatg tggccttacc
     5581 cttgacagac ctcctgtaga tctttcggcc ggcccaggcg aatatgaaaa gaaccagagc
     5641 catggctgcc agtggaagaa gtatggccat tagcgaaaga aagatcgagc cgccagattc
     5701 agcggtggag accacggggt tgccgagccc cccggttgtg aaggccgatg ccgccctggt
     5761 gagtaccgtc gccccctgaa ccaaacctgc cgcggtaccc cctgttatag cggagatgct
     5821 ccatttcagg aagggggtca tctcgggaag gactgaagcg gtgatgaaag tgcctgcgat
     5881 gatcgctgcc ggtgtagcca cggtgtcaag catgttgtcg aaccatggga cgtagtatcc
     5941 ggctatctca aagaaagtgg cggtgcccag cgcgaccgtc gccgttggag tggcaaccca
     6001 ctggaaacct tctgcaagct cgatatgtcc cgtctgcgct gcaaggctca tgaccagcag
     6061 gggtacgaaa acccggaaac cacaggaggc acttaaaccc agtccgacga aaagagcaag
     6121 aatgttttcc atgatatgta ttgtagaccg attctatcgg tccgggtaat taatatacag
     6181 tttaatttaa aaggtgaaga gttgggatga gtatctctaa ggaaatttat tctttctaag
     6241 aatataggaa tataatatta ttttagggat acttaccatc tgcctgtcag ggaggggaaa
     6301 aaatgcatga cattgaagcc ataccaagtc cgggaaggaa gtaccgaaaa agagacctag
     6361 aggggcttga ccctgtcctt cttggagcgc ttctcagaga aagaatccac cataacatag
     6421 aggttcctct atattcaatg ctacagaggc gacctttcaa ggctgtcccg aattttgggc
     6481 ttcaggccca actcgtctac gatgagtggg tgcgtagagg tcttccccag gatggcccgg
     6541 acctggattg ggcaaaacgg tatatcgata tagccgcgca ggtaagaaga ggcgaaaggc
     6601 ccggtatacc ggaactggta gtttcgattc ccgaaccttt cacgaaaagc gagatggagg
     6661 ttgtccgaaa attaatctgg ggacgctgtt ccatccggaa ctggacttca aggccagttc
     6721 cagaagatct gatcgaaaag atcctcgaag caggcagggc tgccccggta gggtgcaacc
     6781 ttgatgctgt acgctttgtc gtccttagga cccctgagga aaaaagcatt atctggagcg
     6841 atatccccac cgacaatgca gtgatcatag tgatctgcta cgaccggcgg atagcggaaa
     6901 ctgtcggtca ggacaagatg gtgccccaga acctgggata cgatgccgct gccgctggag
     6961 atcacatgtt gctcatggct catgcccttg gccttggggg ggtgtggctt tcgaagaccg
     7021 ccaagtcaga acatactgag gatacgggca agatattcaa ggaactgtac ggacttccgg
     7081 accacatcga ggtggcgctt catgttgccg tcggctggcc cgcctccggg atcataaaaa
     7141 gcaaaagaat tcccctgtct gaaatgatca ttgcaaagaa ataaaattct cttttacaca
     7201 actgtatctc ctgctgacgg ggagtagggt ggaccttgtt cccatcacaa ccctactccc
     7261 ccaaaacccc ttcaccccta aagatcccca aaaaattgat ccactcccaa ggctagttca
     7321 gtgagataga gagacatgct aaggttagat tttcttcgaa aaattcccta tcttttatct
     7381 atccctccag cggaaggggg gttcacagac cccgtagaag gctgccttct cccccttcat
     7441 gaaggcccag ttctggttgg cgtagtcgta gtgccaccac tcatcgggta agttggtgaa
     7501 acccgctccc gtcatggcct tgtacaggat cctgcggttc tcaagtacga tgagggtatt
     7561 ctctgaagtt gctccccccg acaagacctt gttttcgtag tataccgttc cagacctgtc
     7621 ggtggtctcg tcgaacgcgg agcccatgtc aagcaagaag ccgtccttgt cggcaagggt
     7681 tatgtcgacg gcacctcccg ttacgtgggg tgaaggccgc ccgggatccg tcgaaggagc
     7741 agctacgaaa cgggatgcca tacgggcgat atagtcgcca tccttgtcgg gcatgatctc
     7801 cctgagttcg gcgcagtacc ttttgaagag caaggactgt agcgaaggag gccgccatcc
     7861 gtcgagcagg accagccgaa gaccggacgg aagcagcctg gcggcttcca ggagcagcga
     7921 cagtacccgg cgcctgacgt aacactctgc tatggctccg tcgagaccgt taaggaaata
     7981 gacagggtgg gaaagaagcc tctccggcac aaggctagcc gggaccagtt cctcccggca
     8041 ctcatctatg ggcactgatt gtgcttcttc ccagtcgaag ggttttccct ggggtatagg
     8101 tggccatcct ctttctgttc ccatttcgtt caacccccta gaggttcagg atccacggta
     8161 tcaatgtggt gagaaagggg aaggttataa ctgccatcag cacgaccagc gccactccga
     8221 tgaagggcca tatgtggaca aaaacgtcgg tcagcctgac cttccctacc gccgccacga
     8281 tatagttgca ggcccccatg ggcggcgtta tcagtgcgat ggtaacgtta agtgtcatta
     8341 ccacgccgaa ctgcaacggg tctattcccg ccttcaatgc aatgggggca aagatcgggg
     8401 tcagaagggt cagggttgag atctcctcca tgaacatgcc gacaggaaat atcaccgcgc
     8461 tgaggacgac cagaaggagc cagggcctgt ccatgagacc cgcctccagg atgaatgccg
     8521 cgaagcgttg tggtatctgc tcccatttca gaagccatcc aaccacagtt gcagctccca
     8581 ttataaggaa gatcgaactg gtaagccgaa ccgtctcgta tagcgactcc ccaagggtcc
     8641 tccaggtcag ttgtctcgtg tagaaaaccc ctgtaacaag gcaataggct actgccagag
     8701 cccctgattc ggtgggagtg acgatccctg ttatgatgcc ccccagtata ataagggggg
     8761 caagaagtgc cactgccgac ctggccagga tctccttttt gcgtttgcgg acatcctcga
     8821 gatcggtgac cggaacatgg taaataatgg aataaaagta attcatcacc atgaaggctc
     8881 ctcccaggac caggccagga agtattccgg ccgcaaagag ccccccgatg gaggtgttgg
     8941 tcatggtggc gtatatgatc atgaagatgc ttggaggtat tatgggaccg atcagtgcgc
     9001 ttcccgccgt caggccggcc gagtaggagg gagagaagcc gtccttgacc attgccggga
     9061 tcaggatcga tcccagtgca gaagcgtccg cagcggcgga tccgttgacc cctgagagga
     9121 taatgcttcc caccacattc acgtaaccta gccctccgtg aacggagccc accagcagcc
     9181 tgcagaaatc aagcaggcgg tcagtcagtc ccgagcggtt cataagtatt cccgcaagta
     9241 tgaaaaatgg aatggccatc aaagtgaagg aatccatccc gccgaagaac tgttggctca
     9301 tcaccctcag cagggagaga tccccaagcg ccaaaattcc tgccagcgat gccgaaagga
     9361 gcgacaggta gaggggcatg ccgaggacca tcagtaccag gaaggaaaac agcatcaaaa
     9421 aacccatatc agctctccct tcccctgaga tggagcagca ccgtccccgc aaggagcagg
     9481 gcgaaacccg ccggaagcgc catcagagga aaggtcttgg ggatgttcag ggccagggtc
     9541 ttcatatgcc agatccttgt ggcgttcacc cagccgagac agatcatcag gataagaata
     9601 gccaccgaaa gaaggaatct gcccaattca acggcccgtt gcagatattg cgggaaacgg
     9661 gggaccacga aatccaccgt catatggtcc cccctgactg cagcgcccag ggccccgagc
     9721 atcactatcc agaccaggga gaaacgggcc acctcttcgg tccaaataag ggaacttttc
     9781 aggacatagc ggcagaagac acccaggagg atgtccccga cgttgactgc gagcatgaag
     9841 caggcagtcc aggcgcttag tgtctccagg cgggttatcc atctctcgag ccccttctcc
     9901 ccttggctcc ccgaaaacat catttcggac ctattttgac ttttcgttgg cgttccttgc
     9961 gcactcaagg gccaggtcga gccagtcctg tcccacctta gccttcagcc actcaacgta
    10021 ggccggctgt cccgccttgc gtaaagtctc catctcgtcg tggttcgggg cataggcgat
    10081 cattcctttt tccaccagga agtcgatgtc cttctggtcg gcatcctgca tggcctgcct
    10141 gttaagcgcg ttcgccttct tcgcggcctc gatgatgacg ctcctttgtt cggcgggaag
    10201 cgagtcgaga agatcaccgt ttatcacaag gaactggtcg gagtactgga tattggcaag
    10261 ggtgagatac ttctggacct cgtagaggct tcccatcttg atatacatgg cagggttcat
    10321 ctgcccgtcg acaacgccgg acttgagagc catgtagagt tcggtccatg ggatgggtgt
    10381 tccgctggcg ccgaatgatt cgaagaggat cacctgtccc tcgtccatcc cgcggaacct
    10441 gagccccttg aaatcgtcag cggtcctgat ctccttcttg ttgtttgtga aagcaaggaa
    10501 tccgccctct tcgacggcct caagcaggta ggctcctgta cgctgatgga actcctccct
    10561 ggccctgttc caatagcggc tctcgtcaaa aaagaggtga gcggcttcgt agctgtcgaa
    10621 catgaagggc actgccgaag cgtatatctc agggaagacc ggtgccaccc cggcaaagga
    10681 tgccacgttg atcacgggct ggttcattcc ctgcttcgtc agaagctcca ttcgttcctc
    10741 ttcgttcccg agctggctgt cagggaagag cctgattgtc acctcaccgc cggttccctc
    10801 ttcgaccaac ttcttgaagt tgacggcgta aagatggacg gcgttgttat ccacatcagc
    10861 tggtccgttg taggacatct tcacctcaac ggaagccatg gaggttcccg aaacggccag
    10921 gcaaaaaacc aacagcagga ttgcagcgaa aaacttacca tacctcatag tgaacgacct
    10981 ccctcaaaag aatccggatc agggtgtctc gaatgttgag aggcaggtcc atcttaaggc
    11041 accctgttaa atcaatgata ccaaatctga ccgtaccacc ccaaccatag acaagatggt
    11101 cccggaggtg aaagaatagt cagggcacac tacaacaggg tgaagatcga ggagttgtga
    11161 tcgttgacaa aagatgttga aagaaagaag gtcggcctcg cccttggcgg gggtgcggtt
    11221 cttggagcag cccacatagg tgtcctgaag gcattgaagg aaaacaacat aagggttgac
    11281 ctcgtatccg gtaccagcgt cggttctttc atcggcgccc tgtatgcctt cggaatgccg
    11341 tgggaccaga tagacaggat aaccagggaa ctgaagtggc ttgacgtatc aagcatatcc
    11401 atatcaagat acggccttct ctccaacagc gggctcggcg agctgataat ggaacatctt
    11461 ggcgacgtca cttttgaaca ggccaaggtc cccctggcag tagtggccac cgatatatcc
    11521 agcggtgaag aggtggtatt gaccagcggc agcgtggccc aggcggtcat ggccagcagc
    11581 tgcattcctg gcgtattcgt ccctgtcgag atagacggca ggctgctggt agacgggggg
    11641 gtggtcgaga acgtgcccat agacccactg aagaggatgg gggcggagta cgtgatcggg
    11701 gtcgatctca attcgggccg gagggcagaa aagcctgaga acattgtaga tgtactgctg
    11761 aggtcattcc actttctgag gaggtccgcc acacgccgac agacagggga ggctgacctc
    11821 ctgatagtgc ccgacctgac gggattcaac atgtacgacc ttgatcaggc aggagaactc
    11881 ttcgaggcgg gttaccagga agctgcaaaa atgctggggg acttgtgacc attgtttcag
    11941 gggcaaagga tcctttggcc ttgacaggtt ggcctgctgc atgggttttt cccgcaatat
    12001 ttctcctgga ctgtacattg atcggggagt agcttaatat atgttcggac caaaacattt
    12061 tttgtttttt aaaaacgggg gtggccttat gaagaagttc ctaacggcga tgttgattgt
    12121 tttgatgatc tcaggcgttg cctttgccgg cggagaccag aactgtgagc agcaccattc
    12181 tgacaagggc cagggttcgg tcggggccag caacccgggg aacggcgacc agacccagaa
    12241 gggcaagtag ttcggaattt tcaatattct cagtacccgg tacgatagat gttgtccaaa
    12301 aaaggcggtc cctcttgggg gccgcctttc tttggtgcct gttttcgggg tctttcttga
    12361 gttatagttg gaacagcttt tggaggacag taccataatc aggcaaggga gggagatcct
    12421 tggcaaaagt cacgatccag cagctacaga agatgaaaga agacggagag aagatctcca
    12481 tggtgaccgc ttacgatttt tcccaggctg tgctggtgga gaaggcggag atagaggtga
    12541 tcctcgttgg cgactctctt gccatgacca cccttggtca cgagggtacg acggccctta
    12601 ccaccgacga aatgatcgcc catatcaggc ctgtggttaa gggagctcct tctcccatgg
    12661 tgatcggcga tctcgtattt ggttcctaca acgaaagcat tgcccaggcg atccattcat
    12721 cgaatcgcct catcaaggaa ggcgggtgcg atgcggtcaa gctggaaggg gcactccctg
    12781 atactgtccg ggctattgtc gatgcaggga tagccgtaca gggacacctg gggctgaccc
    12841 ctcagacagc ggtccttctt gggggtttca aggtccaggg caaagggcat gacgcagcgc
    12901 taaaactgct ggacgatgcc aggaggcttg aggaggcagg ggttttctcc atcgtactgg
    12961 agtgtgttcc agaggaactt ggaagggtca tgtcgcagat tgtcagcgtc cctgtaattg
    13021 gcataggggc cggaaggttc tgtgacggcc aggtgctggt atttcacgat atgcttgggc
    13081 tcttcgacag gtttactcca agattcgtca agcagtaccg acagatagga caggagatag
    13141 ttggcgcctt ccaggaattc aaggaagaga tcaggaacgg ttccttccca tcggaagagc
    13201 acgccttttc aggtttttca agggaagaag ccgatggtat tcttgctggg atcaagtccc
    13261 gaatgaacta gactgcctcg tcgaactcct cccggtacct tgcaacgcct ctggcgccct
    13321 ccattgccac ccagttgagg atcaggacca tgaatgcctc gtcggggacg ttctcccact
    13381 ggcaggcaat gccgtatttc gaggccatct cgaaaccaaa tcgaggatag tagctggggt
    13441 gtccgaggac aacgacaaag ggacagttac gttctctcag aacctctagg cctttcctga
    13501 caagatgtga accaatacct tgtccctgtc tgtcagacaa tacggccatc ggggcgagtc
    13561 ccatgccctc gatcacgcgg ccggagactt cgatggtgac gggagagaag aagatgtgcc
    13621 ccacgacccg gcccgcttcc agtgcgacaa gggataatcg gtcggggcag ttttcccgga
    13681 gcctgtccac gatatcggcc tccatgggct gaccgaatgc cagttcgttc acattccgga
    13741 ttccctggat gtcttctggt ctttcctcgc gtatctctat cattgttcct cctggagtta
    13801 agggccgtat tgaaattgag gtcattggag aaccatcata ccatctgcaa cttaagtaat
    13861 ccccagattt ggaaggccag tacacgcctc cgggaggctg ttggtcaact tgctgttttg
    13921 aacgaaagaa tgtgggaagt tgcaaggaag gcgaccagaa ggcgaccagg aagagagagc
    13981 cccttgcggt ccaggagggg ataaggcaag gggctctgac agagagatcg tcagccagag
    14041 aaggcataga gagacagcta tttccctggc ctggatgctg ccctttccct gaaattccgg
    14101 atcatggagc cgaagatgcg acgggagagg aaggtgatct agaaacggtc gggacttctt
    14161 gtgaaacatg cagcggcagg aagactcccg ttgttgccag acctaccgcc ataagacctg
    14221 ctccgcaata aaagggtttg aatatcttcc tctgcctggg tttgtgaata tctgtatggc
    14281 aataaggttt tcagatccgg tctatcttgc tcaggtcggt ctctttctcc aggaattcct
    14341 ccttctcctt tttggaggtc ttcctgtagt gaaggtcgca gaattcatcc ccatcgggca
    14401 tgcaacggga gtgcaccagg gcccagtcgg ggttgaaggc catcgatttg gcagggtcaa
    14461 cgtaacagta gatcctgcca agttcctctt cacccatttc tttccaggtt tttcccatgg
    14521 tgcaaccctt aagtctcatt gtctttccct tctccgtctc gagtctttca agattctcgt
    14581 aaaggccgta ggtggggaga tcctctgaat agttttcggg catgttgtcc agtcccttcg
    14641 ccagggttct tttcagcgag tcctccccga tccttttccc gtaatccttg atcgccctta
    14701 aagcaagtct tttgccctct tccacgccga actcatcggc aagggttcgt gcataggcta
    14761 tgtgaagcat ggcaagcctc cggcagaccc gctctatctg ttccctggct tcatcaaaag
    14821 gaacaagttt cttttctttt ttatccaagg cacctttcct cctcaaacca gtcccttggt
    14881 cctgggaccg gtcccgctta ttcttgactc aaaaaccact ccttcttccg gggtattggg
    14941 agataccttt tgcaagaagt tcaacagccc ttttcaggtc cttttccttg agcacgtatg
    15001 ccatcctgat ctcttgacca cccagtcccg gagtggcgta aaacccttct gcaggagcag
    15061 ccataacggt ctccccgtcg tctgaaaact cattaagcat ccagatgatg aacttttcag
    15121 cattgtccac ggggagcttt gccattatgt agaaagctcc ctccggtttt cggcagacaa
    15181 taccgggtat ctcgttgaga ccttcaaaaa gggtgtctct tcgcttcctg tactcctcgt
    15241 tgacttccgc aaggtaggag tccggcgttt cgtatagcgc cgccgcgccc aactgctcca
    15301 gcgtcggcga gcacccccga gcctggcagt attttaaccc aaggtccatg aattcacggt
    15361 tccggctcaa gagacagcct attcttgccc cgcaggcgct gtacctcttt gagacagaat
    15421 cgactatgat aaggtggttt cttatgcttt caatgcttcc gaagctgaga aagggttcat
    15481 ccatgtagac gaactccctg tagacttcat cagcaacaag gagaagattg tgcttcatgg
    15541 ctacatcgga aagcattctc acctcttccc cggtgtagac tacacctgta gggttgcccg
    15601 ggtggcttag aagaatagcc ctggtggaag tatcgatcag tttttctatc tcttcacggg
    15661 atggcagatg gaagccatcc tcggccctgg tcgtgatggg tgtgatcctg gcgcttgctg
    15721 ataccgcaaa ggctgcatag gctggaaaga acggttcggg gacaaggatg ctgtcgccgg
    15781 ggtcacaggc aacattgatc gcaaaattca aagcctcact tccaccgttg gttatgagaa
    15841 catcgcctcg ttcgaactct atgccgatct tggcgtagta ttccagtatc ttgtcgatca
    15901 aagcgagatc tccctgggaa gtgccatagg caagtgtctt gttgtcaaaa cttttcagag
    15961 attcgaagaa gagggtaggg gtctcgatat ctggttgacc tatgtgaagg ggatagatct
    16021 tcttcccctt cgccttcgct cccgctgcaa agggttctag ctttctgatc ggagaagctg
    16081 gcagtgcctt gctgcgtttt gagaattcca ttgtcattcc tcctaagact tccccttggg
    16141 gagagtatac cgaagagcaa ggccctcgtg tatactttcc ccaaggggta ttgttactag
    16201 gcttccggga tataatcgca gatatattcc actatctcgc agcatctgat ctggtagcta
    16261 ctgttgcgga cgacagtaaa ggaatcatcc ttctctaccg ttatccattc cttcttgtcc
    16321 gggggtagaa ggacctcggc tttcccgcta acgattttga ctatctcctt atctcctacc
    16381 tcgaactcaa aagtacctgg gaggattacc ccaagggttt tacgggttcc gtcggggaag
    16441 aatacgttcc tgcttacaac ctttccatcg aaatacacat tggcttttgt ccctaccgtt
    16501 acgttttcaa gcctttcatc catgttttga aggcacctcc tgtattgtcg gtccctagct
    16561 caacaaaagt cgggggagcc agagggcgat actcggaaaa aagacgagga agaccagcga
    16621 aagaaactgg agccctataa agggcaggct ggacatgtag atctcttcta tgcctatatg
    16681 gtcaggtgcc gctcctctca tgtagaagag gctaaagccg aaaggagggg agagatacgc
    16741 cacctgaagg agaaccatga acacaaggcc ccaccagagg ggatcaaatc ccagtttgga
    16801 gaggattggc gagacgatag gagcggcaag catgatcata gccgtcggtt cgaggaacat
    16861 tcccaggaag aacacaaata tgaccgaggc cagaaaaacc atccatggtc cgccgggtag
    16921 ggacatggct atttctgtta ccaacctgtt gccgcccgtg ccggaaaaga ccgctccaaa
    16981 agctgatgcg gctattacta tccatccgac catgctggat atctcgagtg tctccagcga
    17041 tgccgatccg attatgcccc aattaaggcg tcgggtaact gcgctgaaga aaagggctcc
    17101 tacagctccc acagccgcag cctcagtagg cgtcgctgct ccggacagaa ctgaaccaag
    17161 tactaaaagg atcagaagtg atggcagaaa aacctttctg agggcgcgta tcttgccccg
    17221 gaaatccacc ctgtcctcaa ggggaagtgc gggacagacc tttgggttga aaagactgca
    17281 ggtcaggatg tacccgcagt acaaaagagc aaggaatgtc ccacaggtaa gtcctccggc
    17341 gaagaggctc ccgactgata ccccggtaac agcaccgtaa attaccatgt tggtactggg
    17401 cggtatcagc tgaccaagag tcccgccagc cattaccgaa ccaaggctta ttctctgatc
    17461 gtagccatac ttaagcatct gggggagccc gatcaatcca agtccgataa caccggcagc
    17521 cactacaccg gagactgccc caaggatcgc acccaccagt attgtcgcta ctgccaatcc
    17581 ccctctgaga gggccggacc acttgtagaa agagtcgtag atatcctcta ccactctaga
    17641 tttctgaagg atcagcgcca tgaatatgaa caacggaacg gccagcaagg caaagttgtt
    17701 catcgtaccc caggcggctg taacaagaag cttcatcccc ccgactcccc aaagggacca
    17761 tccgaagacc agggcgacaa gggtgagggc gaatgctatt ggtataccca ttgccagagc
    17821 aatgaacaat acaaggaaca taagaagtgg aacgttttcc atgacgtcac ctgctttccg
    17881 ggagcgatcc ccggctgagt cttatgatgt tcttgatcga ggctattagg agaaggagcg
    17941 ccgagatcgg gacaatgcac ttgaaccacc agatgggagg atcgaaagtg gtctgatgca
    18001 tggaacgttc catgatcttt atggattgga tggcccaacc ggtgcttatg tatacgagaa
    18061 ccgccgaaca gaacgcaacg accatttcgg aaagaaaatc catccttcgt ttgttcttcg
    18121 gggaaaggta tttgtgaatg atgtcaacgg caacgtgttt tcctttaaga tgggtataac
    18181 cgccgcccag catgaaatga actccgtaaa ggaaaagaga gatctcgaac gcccaccccg
    18241 gcatgttgtt gaatacatac ctcatgatca ccgcaacaac aataatgaaa gcgagcggga
    18301 gaataatata cgaagtgtat cttcccagtc tttccatgat ctaattcccc cagcaaaaaa
    18361 agaatgggaa gcgcccgtcc gcgaggtatc tcctggtcgg gcacttccct gttcggatcg
    18421 atctacttag ccttgtagcc aagtgttgct gcttcttttg tgtaacccag ttcgttcagg
    18481 acttcagcgt agagctcaag gtattcctgg cattcggcgt tcttcttgcc ccactcattg
    18541 aggacgttgg cagcgatctc gcgcatcttg acaacgtctt cttgggggag agtgataatg
    18601 tcaacgccct tggcgagcca ctgctgcttg gtcttggcgg cttcgctgta gttggtgaca
    18661 gccgaaaggt atctcatgtg atcccttgca gcgagaacaa ttgccttcag gtcgtcgggg
    18721 agttcagccc acttcttctc gttaacgatg agccccttgt cctcgtttgt tccgccgtgg
    18781 tgagccgggt ccatgacgaa cttggtaact tccagcaggc ccatctcttc gttctctttc
    18841 cattcggtcc actcggcggc gtcaatagtg ccggtctgca tggcagtgta gatctccgga
    18901 gcagagagag agacagcgga agcgccgaga gcggtataga actgtgcccc catgccgctg
    18961 gagcgtatct gcttgccctt gaagtcctcc agactcctga tgggagtgct catcatgagg
    19021 atttcgggga cggagcagtc gacggtgccc aggtaggtta taccgtgttt cttgtagacc
    19081 ttttctgcga tcgggaaagc tctctgatca aggtacatgc cctcatggaa agtgaggatc
    19141 ggcgaaccgg ggcgggttgc gaagatggca aagaaaggat ctttcccggc ccagtaggcc
    19201 gaatagatca ttcccatctc aactactcca tcccttattg cgtcgaaaga gtcgaacacg
    19261 gggaaaagga cgcctgcgcc gtagggttcg atgataagcc ttccattgga ggccttattc
    19321 accatgtcgg caaaagcctc aacatgcttg taacggtatg tacccaccat tgaatgcgta
    19381 acaagccgcc acttgtaaac tttttcctcc gcttcggctt ttgagaaatc gccgattgcg
    19441 gtgaagcaga aagcaaatac cagcaccatc gccactagaa ttgaaaatct tctcatctca
    19501 ttaccgcctt ccttattcgg ggtttgaaag atgctgatga gtctggtccc ttctgtgatc
    19561 ccttgctgat caaacttttt ccggaaaacc tcgcacctcc ttccattatc tatatgaaaa
    19621 agtcttgctg gaactgaaaa agtgcccaaa agggaaatcg atgaaaattg tcgttgacag
    19681 ggttatttta taatcctata ttattatagg attgcttaat gtcaatactt ccatgaagag
    19741 tccttgatac gagacacaca aaaagttctt tgtagcctga cttggaaaag gactttttaa
    19801 agaaatacaa aaaaggaggc gttcaatgtt gctggtggaa ctccggatac acggaagagg
    19861 cggccagggc tctgtggcca tggcggaaat gatagcgggg gcggcttttg ccgaagggaa
    19921 gttcgcccag gcttttccat acctaggtgg tggcggggaa aggagaggag cacctgtcca
    19981 ggcttacgcc aggatctcag attccccgat caagttgcgg gagaagatag aggagcccga
    20041 ccttgtgatc gtccaggatc caacgatcat ggagatggtc gatgtccttt caggactgaa
    20101 gaaggatggc gtgatccttg tcaattcgga atcatccatg gagttatccc gggatgatgt
    20161 aagggccttt tttgtacctg ccagccgtat agcgatagag accatcggca ggccgataat
    20221 gaataccgcc ctgataggag cccttgcccg gatcaccggc ctggtgtctg tggatgcggt
    20281 tgccgaagct ataagggaga agttcccagg agagatagcc gaaaagaacg tgcttgcagt
    20341 acgtaaggcc tacgaacagg ttggaggtgt cgaataatgg acctgccgct cggtggtgta
    20401 tgcaaggctg gtgaatccaa gaaacttgaa acaggaaagt ggaggtcttt ccgcccggtg
    20461 gtggacaagg caaagtgcat aaagtgcgga agatgtgccg aattctgccc cgattcctgc
    20521 atggagataa aagaggacgg agcagaggtg aacctctatt actgcaaggg ctgcctgatc
    20581 tgtatgcgtg aatgccctgt cgatgcgata tcgaaggagg ttgaagagaa gtgaaaggca
    20641 aaaccgctat tctcgaaggt tcaagggcgg ttgcggaggc agttcgcgca tgcaggccgg
    20701 atgttatctc cgtctatccc ataacacccc agactcacat tcttgaggac cttgccaggt
    20761 ttgtctcttc cgggaaactg aaaagccagt tcgtgagggc cgattccgag ttttcggcag
    20821 cctccatagt ctacggggca agcgcggcgg gggcaagggc ctacacggcc tcggcatccc
    20881 agggtcttct tttgatgaac gaagtgatct atgccatggc ttcgacacgc ctgcctgtcg
    20941 tactgacggc tgtgaacagg gccatttccg cccctatcac cattcagccg gatcaccagg
    21001 atacgatgag cctcagggac accggtgcaa tccagatcca cgtggaaagc atgcaggagg
    21061 cctacgatgc ccatgtgcag tcattcagga tagcagagga tcccgaagta cagcttcccc
    21121 ttatggtgtg catggatggc tgggtgatca gtcactccta tgagcctgtt accgtctacc
    21181 ccgatgaggc cgtggcggag tttgcgggaa gcttcactcc cgtccagaaa ctggacccct
    21241 cgaacccttt gacctacggg gcctacgcag accaggatgt gctcatggag tacaggtacg
    21301 ccatgatggt cgcccaggag aaggccaggg acaagatccg ctcggtaacg aaggagtaca
    21361 gggaggtctt tgggaatttc gacggtgacc ttgtagatac ccactgcacc gaggacgccg
    21421 aggtcatcct cgtggctatg ggttctatgg tttctacgat cagggctgcc gtggatgaac
    21481 tcaggtcgga ggggaggaag gtaggtctca tcaaggtaag gaccttcagg cccttcccga
    21541 gggaagagat catagcggcg tgcggcaagg caccggtggt ggcagtgctc gacaagagcc
    21601 ttagtgtcaa ctccgggggc attctggcta ccgaggtcaa gtcggccttt tacggggaaa
    21661 acgtccagcc ggtcatagtt cccttcatgg cagggcttgg aggtaaagag gtcaatcgca
    21721 gggtcgtaaa ggagatcgtg gacatcgctt ccagggctgc agagaagcag ggagcccagg
    21781 aaacggtctg gatgggcctt gacagaagtt ttttcagctg atcgcgtaaa gggatggtga
    21841 ttaaagatga caattcagac agaagttaag gaacttcatg cccccggaca ccgtgcctgt
    21901 cctggatgcg gatgcgcgat cgcggtgaag ctcatcctca gggaaacggg gcctaacacg
    21961 atcgtcgtat ctccgacagg gtgccttgaa actttcacaa gccccttcag gggttcctcc
    22021 tgggaggttc cctggataca ttcccttttt gaaaatgccc ccgccatcgc gacgggtgtt
    22081 cttgccgggc tcaattcgaa gggcaacgtc gacggcacaa agattgtagc cataggcggg
    22141 gacggaggga cctacgatat aggtatgggc tccctttcag gcatgttcga gagaaaggac
    22201 gatatcctct atatctgcta tgacaacgaa gcctatatga acaccggcat ccagggaagc
    22261 agcgcaacac ctctaggtgc cggtaccacg accactccag tggtcggtaa ctctttcggc
    22321 aaaatggtca gcaagaaaga cctgttggcc attgcccttg cccatgacgt tccctacgcc
    22381 gctacggcca gcatagcaga tcctgcggac ctgaagaaaa aggtccgcaa aggcatggat
    22441 aaccctggtc cggcctttat cctgatgctc acgccatgca accttgggtg gggattcgag
    22501 ccggagaata ctgtcgaact ggccaggaag gccatctcta caggactgtt ccccattgtc
    22561 gaattcgaga atggccggct ggtgtcggtt cagaagatca atcctgttcc tgtggaagaa
    22621 tacctgaaac cgcagaagcg ctatcagcac ctgtataaaa gagatgattt caatgaggtc
    22681 ctgaaagcga tccaggagaa ggccgacagg aacatagaaa agtacgatct tgcgaaggat
    22741 cggaactgaa cgaaaagatc tcaagagggg gttatcctga tgaaaaaggt aatggtctac
    22801 ggaacaggga ccatgggcac cggaatagca caggtactgg cttcatctgg agtggaggtt
    22861 cttctggtct ctgcccgtgg aggtacagag aattcttcaa ggggtaaggg caaggtcggg
    22921 tctattttgg acagagccgt ccagaagggc aaagagaccg cggagaacgc aaaggtgatc
    22981 atggacagga tccaccctgt ggccgatatg gccgagggca tttccggggt tgacatgtta
    23041 attgaagcag cgacagaaga gatggaactc aagaaggaag tattccggaa ggctgaaagc
    23101 ctggatcccg gtgaaaagat cattttcgcg accaacagtt ctgccctgag catcactgaa
    23161 ctcgctaccg ttacgaaacg gaccgaccgg ttcctggggg tgcacttctt caaccccgcc
    23221 ccggtgatgg cccttgtgga ggtggtcaag ggggcggcca ccgacgaaga tgtcgtggcg
    23281 gaagttgtta ccttcatcga atccctgggt aaatccccta tcgtggtaag cgaggctcca
    23341 ggttttgtgg tcaacaggat ccttgtgccc atgatcaacg aagcggtata cgccctgatg
    23401 gagggagttg cctcggctga ggacatcgac aaggggatga aactgggtgc caaccatccc
    23461 ataggccccc ttgctcttgc ggaccttatc gggcttgatg tctgcctttc cgttatggag
    23521 accctttaca aggaattcgg tgataccagg tacaggccct gcccccttct tagaaaaaat
    23581 gtcagggcag gcttcctcgg ccgcaagaca aagaggggat tcttcgttta tgagtaattc
    23641 cgtaaaccag atccagacac aggaggtgac tttgctatga aaaacggcca ggagtatatc
    23701 gaaagccttc gaagcctcaa gccggtcgtt tattacctcg gggagaaggt tgacagcgtg
    23761 gtcgatcatc cggccttcca gccccatgta aactgtgctg ccctgaccta tgaacttgcc
    23821 atgcgtcctg aataccagga actgttatca gcgcgctcac cgtttatcaa cgaccgtgtg
    23881 aaccgctgga cccacatcca cgagaacaat gaggacctgg tcaaaaaggt gaaagctctc
    23941 aggttccttg gccagaagac gggggcctgt tttcagcgat gcgtgggctg ggatgccctg
    24001 aacgctgtct attcggtaac ctgggagata gaccgggaca agggcaccga ctatcacgca
    24061 cgttttctaa agtaccttga gagagtgcag agggatgacc tcatgatcgc cggggcaatg
    24121 accgatccca agggagacag gagcaaaaaa ccgggccagc aggaagatcc cgatatgtat
    24181 gttcacgttg tggagaaaag atccgacggc attgtagtca gaggggctaa ggcgcatatg
    24241 accggaatgg tcaactccca tgaaatgctg gtaatgccga ccacgggtct ttctgaagat
    24301 gacgccgatt acgcggtttg tttctcgctc cccgtcgacg ccgagggagt gatacatatt
    24361 ttcggacgcc agaccaacga caccaggcgc cttgaaaaga gcaccattga caccggcaac
    24421 caccgtttcg gtatcgtggg aggggaagcc ctcacgattt ttgacgacgt ctttgtgcca
    24481 tgggagaacg tctatctctg cggtgaggct gagtatgcag gccttctggt ggagcgtttc
    24541 gccacacttc accggcagaa ctacggcggc tgcaagaccg gcgtcagcga tgtcatcata
    24601 ggtgccacag ctgcccttgc cgactataac ggagtcgaga ccgcctcgca cataagggat
    24661 aagctggtag aaatgacctt tctggctgaa acactttaca gctgctcact tgcatgctcg
    24721 catgagggac acacaactcc gtccggtgca tatctggctg atccaatgct ggcaaatatt
    24781 accaagcaga acgtgacaag gaatatctac gagatatgcc gtctggccca ggacatcgcc
    24841 ggcgggctca tggcgacgct tccctccgaa gccgacctca acaacccgga gatcgggccc
    24901 ttcatcacca agtacctgaa gggccgagaa ggtgtcgata ccgagaccag gataaggatg
    24961 atgaggctta tcgagaacct taccggcggt actgcccttg tcgaatcaat gcatggggca
    25021 ggatcccccc aggcccagaa aataatgatc tacaggaact ccagcatcaa ggggaagaaa
    25081 aaacttgcgg agatccttgc aggggtcgat ccggatccag gcgaaagcac ccggggatag
    25141 gcgtctcggc ggcctgttct taaagaggag gttatgggat gtctgttacc agtgagaagg
    25201 aatttgtttt agttgaggat gctgctgaag gtgttctgtc agtcgcgatc aacagcccca
    25261 ggaccctcaa tgctctcaac gcccggatca tcggtgaact cagggaaact ttcagggagt
    25321 gtgccgcaag gtcggatgtg agttcggtct tattgacagg gaagggaaag gcttttgtcg
    25381 caggtgccga tatatccgaa ctgtcaggtc tcactcccgg ggaagctgcc gctttttcca
    25441 ggagcggtta tgaccttttc cagatcattg aggagatgcc ccagcccgtc gttgcggtta
    25501 tcaacggctt tgcccttggc ggaggtcttg agcttgcact ggcttgtgat tttcgctttg
    25561 tggcggaggg ggccaaactg ggtctcccgg agatcagcct ggggataatt ccgggcttcg
    25621 gggggaccca gaggctggcc tcgatcgttg ggatatcgaa ggccagggaa atgatactta
    25681 ccggcaacag gtttgacgct gcctggtcgc tggcatgcgg tcttgtggac agggtcttcc
    25741 ccgaggacag gctttgggat gaatcgctct ccttcgccgg tttgctcgcc gcacgcagca
    25801 gcagtgccat gtcgcaggcg aaaaaggtcc tgaacctttc caggaatcac cctgtcgaaa
    25861 agggactcga gatagagaat atggcttttg cagcctgttt tgaacatccc gacagtcatg
    25921 aagggataaa agcctttctg gaaaaaagaa aacccaggtt cgcctgagaa ggaaatcctg
    25981 aggggcagga agaaaatccc catgaaaaca ggagctgcag agaaggagga aagaacgaaa
    26041 tgagtatggt ctctcttgta aaggtcaaac aggatgatga gaaagggata gctgaagctg
    26101 ttcgcagggc agtcgatatc gtcggcggcc tggatgatat tgtaaaaaaa ggtgatcttg
    26161 ttctcatcaa tcccaatctt gtggctgtcc catcgggccg ggtaacgggt gcgatcagtc
    26221 gctgggaagt ctgcaaagct attgccgacc tggttatgga aaagggcggc cggccggtaa
    26281 tagccgagtc ttccgccgcg ggggtggaca cggaaaaggt catcgaagcc ggggaatata
    26341 cccggctcag ggaacaaggc tacgaagtga tcgacctcaa acctaccccc cgggcaaaga
    26401 tccctgttcc ggaagggcat aaggtctttt ccgaattggg atcctgggaa ctggtccgga
    26461 aagcagacgt gataataagt gttcctgtca tgaagaccca tgaccagact gaagtgaccc
    26521 tgagcatgaa aaacctcaag ggccttattg atgaccccac gaaaaaggcc ttccacaggg
    26581 aaggactcct ggaaggcgtt gtagacctca tgaccgctct caaacccaaa ctgaccgtct
    26641 ttgacgcgac agtatgccag gaaggaatgg gacccatctt cggaaccccg gtacccatgg
    26701 ggcttattct ggcttccagg gattatgtgg cagctgatgc ggtaggcggg aagataatgg
    26761 gatacgaccc caaggatgta atgataaccg tccgggctgc ggcaagaggc attggggaga
    26821 tggacctcga caggatagag gtcaggggag atgcttccat cgctgatgta gagagacggt
    26881 tcaagcgtgc gagcgaaacc gttatcgagg gtgttccccc attcaagctc atgcttgatg
    26941 aaggcgcatg caccgggtgc cggaacacgg ttatcagcgc gatcatggat ctcaagagcc
    27001 agggtcttca gggctgcctg gagggcaagt atgttgtagc aggcccgctt gacgagaaac
    27061 ttctaccccc ctgcgccgaa aaggataaaa cagtattggt gggtatctgc gcaaaacatc
    27121 tggaaggccg cggaacatac gtccagggtt gcccgccgaa caacattttt atagtccgtg
    27181 cgatcgtagg tgaggggcag aagatcgaga ggcgctacgc gacggaagag ggggccgatg
    27241 atcagtcagg agagacgaaa taacaggtac agaaaactgc cgtctcaaaa tacaaaagta
    27301 atgaacggag ggaacggctt tgagagaagt tgtagtaatt gatgctgtaa ggacccccgt
    27361 agggaaattc aatggaggtc tttctgccgt aagcgcgtca gatctggggg caagggtgat
    27421 cagtgccctt cttgaaagga ctggagtcga ggcctcggcg atagatcacg tgataatggg
    27481 caacactttg cagtttggag agaataccta cctgagcagg ctggcagctg tcaaggcagg
    27541 gatacctcat gaagttccag cattgaccgt aaaccggctc tgcggctccg gtctggaagc
    27601 catcaatgag gccgttcgct tgatccagct cggattggcc gatacttgcg ttgccggcgg
    27661 ttccgagagc atgacaaacg gccctttctt ctccg
//
LOCUS       DAOTIQ010000018        26876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2064_length_26876_cov_7.528839, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000018 DAOTIQ010000000
VERSION     DAOTIQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 26876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26876
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_26876_cov_7.528839"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="P5541_03975"
     CDS             complement(<1..731)
                     /locus_tag="P5541_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71169.1"
                     /translation="MIASVFALLLVDLYILATVFLFRTGSLGDVIHSWALQVPGSAII
                     ILLLFAAWLSLAWLSGLRIPSLARQTFGTAVLFICCSLFLGLYRLSGISSTANLLQPG
                     TLGNAIAMTLFNKIGPLGTVFTGFAGISLAMGLYGVLSRRTFSFLTGIFSESGRNADN
                     QASLSNSAVSEKQATTKPQTGKTPGKIAIPENDLPDGSGKDYLPRSEDPDPELHFPVP
                     LEIFGEHNDYFFDLDRDLLESRGES"
     gene            complement(808..2172)
                     /locus_tag="P5541_03980"
     CDS             complement(808..2172)
                     /locus_tag="P5541_03980"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRV71170.1"
                     /translation="MDRLFDIMETLKIPGITFSDAFIQRSHGRYALFDNGKFEEISAS
                     RTDGTGIRIVMGDRTFFTHSTGATFNRALDCISGSSERAGFSRTSRNASRDDLLLPDP
                     EIPDDDPLERIRDIDKAIRKESNSIKQVTLGWGSASSEIAIAGTENPLCRFSSSKTRF
                     TVSVILEKNGALFTGREVRALSISAGEFLDVVDLESVATEALRRAILMSEALPCPAGS
                     MPVVISGMAGGTMIHEACGHSLEADIILKDFSVFRDSMGKQVASPLVSLADDPMIQDL
                     FGSYPFDGEGTPSKRTILVRDGILESFLTDIHSSIQGDFPRTGNGRRSSYKVSPVPRM
                     SNTFIMNGTEEPESLVARIKKGLLVKKMGGGEVNPTSGDFVFHVTEGYLIKNGRPVWP
                     VKNAILTGSGPQVLMNIEAVGNDLHFESGMCGKSGQNVPVTDGQPSLLIREMTVGGSD
                     TEGN"
     gene            complement(2200..3207)
                     /gene="mltG"
                     /locus_tag="P5541_03985"
     CDS             complement(2200..3207)
                     /gene="mltG"
                     /locus_tag="P5541_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRV71171.1"
                     /translation="MLFFVTAAFYVSLTWIVPERLSVIIEPGRGEPQEFVVEPGMGAL
                     DIARLAKREGLTDSPSDLARFFFKLGIDRSIRPGIYRIRPGSPWEIARQIERSEPEIS
                     SMTIIPGESLPEIAQKIGDTIHVAIQKDSLFPEEVRRFLPADPLSRLAFLLPDTYSVS
                     PTVWAAEELIKSASFAWWKRVGSRIEEELALDSKNLLKTAILASMIEKEARQEKEKKI
                     IAGVILNRERSGMKLQIDATVVYAWALRGLSLKRVLYSHLEIDSPYNTYLYPGYPPAP
                     ICVPSASSWDAALNPDFVPYMYYVARPDGSHFFSVSYNEHLGAIKRARSEFDSLMNTP
                     E"
     gene            complement(3258..3893)
                     /locus_tag="P5541_03990"
     CDS             complement(3258..3893)
                     /locus_tag="P5541_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRV71172.1"
                     /translation="MNFHEMKLTDFLEELGSSSPAPGGGSVAAVSGALGASLARMVAN
                     LTVGREKYAESEEAMTRVRESAIKLAQDFLEQASEDTKAFTSFMKALSMPKNSDTEKS
                     ARKEAMQGALKMSTEVPLRILCLSRELSELCLTAAEMGNPNAITDAGVSALMAGSAAK
                     GAALNAQVNLSSIDDPDYRKTSNAIIERELVSVEITVSKVLVRVASSLDPS"
     gene            complement(3890..5152)
                     /gene="hutI"
                     /locus_tag="P5541_03995"
     CDS             complement(3890..5152)
                     /gene="hutI"
                     /locus_tag="P5541_03995"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRV71173.1"
                     /translation="MNAKLFMNGKIFTPINDGASGSGIVFYERGALLVRDGLIIEAGN
                     IEDVLAHPLSAETDQQIDCMGKCVIPGFVDPHTHMCFASLREQEFAMRLEGTPYLDIL
                     KAGGGILSSVKNVRESSDEELFRVTRKNIETALRHGTTTIEIKSGYGLDTPSEIRMLN
                     VIARASRETPPDIVSTFMGAHAVPQEFRDDPDGYVDLIVKEMIPAVAKECSPSFCDVF
                     CEKGVFTIPQSRRILEAGSNHGMGAKIHADEVNDIGGAALAAEIGAISAEHLLAASEK
                     NLEAMSAAGVIAVLLPATAFSLKKPYANARRMMDLGLTVALSTDCNPGSSYTHSMPFV
                     FSLAVMNMAMTTEEALMGCTLNAAKAIAMDSRTGSLEPGKFADLVILDGETPAILAYN
                     SGVNPVLSVYKKGEPVVSKLCEWRRETC"
     gene            complement(5152..6060)
                     /gene="ftcD"
                     /locus_tag="P5541_04000"
     CDS             complement(5152..6060)
                     /gene="ftcD"
                     /locus_tag="P5541_04000"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRV71174.1"
                     /translation="MKIVECVPNFSEGRRKEVIEKILSPFRGGRGFTFLDARADEDHN
                     RLVVSLAGEPEPLQEALLKAAAEAVSHIDMNVHRGGHPRIGAIDVVPFTPLGTSTMEE
                     CVQIAHSFGKRLNSATGIPVYYYEEAALSPERKNLENIRKGQYEALINNVKEPGRHPD
                     VGEPSLHPTAGATVVGARKFLVAFNVNLGTDKIDIAKSIAKSIRASGGGLAHVKAIGL
                     PLEERGLVQVSINIVDHEKNPLYRVLDMVRNEAARWGIPVLETELYGMVPVSAMYESA
                     VRYLQTVDFDPSQIIELKLLEMIGEE"
     gene            complement(6076..8088)
                     /locus_tag="P5541_04005"
     CDS             complement(6076..8088)
                     /locus_tag="P5541_04005"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRV71175.1"
                     /translation="MKNNNIVEKAMKIRLDPDLPEMPAFDPSIRRAPDRGYRLTADQT
                     LVALKNALRYIPPEHHDTVAPEFLEELRTRGRIYGYRYRPDGSIKGMPVDFYKGKCLE
                     GKAFQVMIDNNLDTEVALYPYELVTYGETGSVCQNWMQYRLIKKYLENLTQETTLVVE
                     SGHPLGLFASSPGAPRVVITNSLMVGMFDNPDDWEIAEQMGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRKLGLGSESGLEGVVFVSSGLGGMSGAQPKSAEIAGCVGIIAE
                     VDMSRIKTRHDQGWVSVVSSDLPEVFNIANKYREEKKGISIAYHGNIVDLLEYALNHN
                     IPIDLLSDQTSCHAPYEGGYCPWQLDFAERTEMLQKDRNKFRELVNSSLGKHFDLIRK
                     LVNKGTYFFDYGNSFMKAIFDAGYTEISINGKDTRDGFIFPSYVEDIMGPELFDFGYG
                     PFRWVCLSGKPEDLEKTDNAAMECIDRDRRPQDMDNYIWVRDAGKNRMVVGTQARILY
                     QDAVGRVKIALRFNKMIRDGDIGPVMLGRDHHDTGGTDSPFRETANIRDGSNVMADMA
                     VQCFAGNAARGMSLVALHNGGGVGIGKAINGGFGLVLDGSERVDEVIRKSITWDVIGG
                     VARRSWARNPNSIDTIVEHNNSTDDSITLPYLANEEMLLNLIVKQP"
     gene            complement(8116..9648)
                     /gene="hutH"
                     /locus_tag="P5541_04010"
     CDS             complement(8116..9648)
                     /gene="hutH"
                     /locus_tag="P5541_04010"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRV71176.1"
                     /translation="MVSSILELNGHSLMLEDIVQVARMNKKIALDSSAVAFVERGSRM
                     VRSWVDEGKTIYGITTGFGDLASQVIPADKSEKLQENLLVSHACGVGDPFPEHIVRAI
                     MLLRINSLIRGFSGISMKTLSRLVDLLNLGIHPVIPCKGSVGASGDLCPLSHLAIALL
                     GIGDVFYKGKQISASEALRINGLEPVRLGPKEGLALNNGTAAMTGVAAMATWEALSLL
                     KTADIISALSLEALRGVPYAFDSRTHELRPHQGQIRVARNIRRLIEGSQIVEKHKKER
                     VQDAYSLRCIPQVHGASRDGIDYVHEKIQIEINSVTDNPLIFPAEGEAISGGNFHGQP
                     VALAMDFFGIAMAEIGSISERRSARLVDGKLSGLPPFLVANSGINSGFMIPQYASAAL
                     VSENKVLSHPSCVDSIPTSANQEDHVSMGMYSARKGREILDNVKNILAIELLLASQGV
                     DFSSPLKPGAGTAVIHQLVRTEVPHLNEDSFLHPMITKVIQRIEDGSYLRGVEDAIGI
                     LE"
     gene            complement(9703..10704)
                     /gene="amrS"
                     /locus_tag="P5541_04015"
     CDS             complement(9703..10704)
                     /gene="amrS"
                     /locus_tag="P5541_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRV71177.1"
                     /translation="MTEKTETRAKYWKKENNGNTRCLLCPRGCLILPGEKGNCLTRLN
                     RGGELFTLNYGYFCASVDPVEKKPLFHWKPGTEIFSVGTFGCNLFCPFCQNAHLARSE
                     TPTGLKYLSPNGFSSLVRDSGQSSVAFTYNEPSVWFEFILDISEPLKKYGADIVLVTN
                     GFISPDPLEELLDITGAMNIDLKGFYPRTYEKLGGSVGPVLHWIKRAVERGIHLEITH
                     LVVPGINDSLEEFLSLTRWISGLSNNIPLHISRYFPSWQWQEPPTLLQEIRKRKEIAS
                     RYLNFVYTGNTGEKNDTFCPSCGKLVITRDYGRSVETIATPEGNCPFCKTPLGIVTR"
     gene            complement(10701..12023)
                     /gene="amrA"
                     /locus_tag="P5541_04020"
     CDS             complement(10701..12023)
                     /gene="amrA"
                     /locus_tag="P5541_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRV71178.1"
                     /translation="MWSAGLLLPHAPILVPEVASRSGVRPGKTLEAIVSMKTKLKGHT
                     PEAILLLDPHAQTGLKPCVVNSYRFKGSLSGFGAGNQSVDATGVGKEASSLLEHINQM
                     FSIAEQKPDVFTLDYASVVPLLFFEMIFGKIPPLLIFNPLLLDFSSAYKFGIHLRRYN
                     SSTDWLLLASGDLSHRLKKDSPAGFHPDGEILDRKIQESIVSASPEPVFSLTVRTILN
                     GGECGLRSVLALIGISLGEEIELLSYEAPFGVGYATALWKPERPAENVGKYHLILARK
                     AIEMASLGKDPNDHLEEMPLFSDKSLEERKACFVSIKTVGGELRGCIGTVVPVFPSIG
                     EEIVSNAYSAACKDPRFMPVAWNELLNCRISVDVLDEPEPITDLSLLDPKVFGVIVEK
                     GSRRGVLLPDLDGVETVEKQLGIALSKAGIGSAEGVRISRFRVSRFSE"
     gene            12130..12600
                     /locus_tag="P5541_04025"
     CDS             12130..12600
                     /locus_tag="P5541_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRV71179.1"
                     /translation="MGPPEGNQKESRVLDLIGRLEELIEVFQLTESDFSELRDIPFPK
                     RKLSAIAGVLALPLSQAARILLISSVLQSRGDELNCRFNELASVSGLSERTAYRALKE
                     IKESGLFDIKAVPGTGVTIYSRWLWSKPGERALRILGENRDIQDQSISMESPKE"
     gene            complement(12567..13643)
                     /gene="queA"
                     /locus_tag="P5541_04030"
     CDS             complement(12567..13643)
                     /gene="queA"
                     /locus_tag="P5541_04030"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRV71180.1"
                     /translation="MGKIKSPGNDPRYFNSASYDYFLPEELIAQDPVEPRDSSRLLVI
                     DRSSGEMYHRYFRDITSCLRKGDIMILNDTRVIRARLIGKKEGGESRIEVFLLKPVAM
                     QNSVAVEWEALIRPGKRVKPGRRVILKGDIPVTVLGEGNDGTRICRFPDDLDVMSFIE
                     KNGEIPLPPYIKNSAIDPERYQTVFSRNSGSVAAPTAGLHFTRPLLEKIKSIGVLQKN
                     ITLDVGLGTFRPVKTSDIRDHAMHIEEGFISEETATEIMNTRKRGGRVVAVGTTAVRA
                     LESRTIDNGELEAGPFSTDAFFYPGYRFRMVDVMVTNFHLPKSTLLMLVAAFAGLDLT
                     MKAYREAVSMGYRFYSFGDSMLIL"
     gene            complement(13644..15065)
                     /locus_tag="P5541_04035"
     CDS             complement(13644..15065)
                     /locus_tag="P5541_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRV71181.1"
                     /translation="MITGNERWRKISLFLSAGIALLLSLTVTPFPPAVASAATISVGI
                     STGESSSSFSAGKESFNISGASGEKASFKSAIKISAGTGGTIVAGSKTMKLPVTIKSQ
                     SMIQWNGRPYRGYMRLVQDGRGFAVVNVIDVEDYLKGVLKMEVNPAWPMEVLKAQAVI
                     ARTYALRNRNKHGSAGYDICATSHCQVYRGVNAEDPILTRAVDLTRGMVLKYQGTIAA
                     TFYHSDSGGHTADVSTVWSSVIPYLMGKPEPIAYESPYSNWQVRIPLSEIQRVLSARG
                     VNVGNLSSIEIASVDMAGRAEMITVSGSSGSVTLKSSQFRTFMGPDRIKSTFFRIGEA
                     VPNLKTNVPQNSTPSSAAQDQDNIPLSPRDEQLLISLTKNGVFNSEELMDMLIKPEKR
                     GHYLRVALERQSGCSNESSLPNPQQPPSRSSGNTVVFTGRGWGHGVGLSQWGAKSLSE
                     AGWGFEKILQHYFPGTRLEKISN"
     gene            complement(15065..16138)
                     /gene="ruvB"
                     /locus_tag="P5541_04040"
     CDS             complement(15065..16138)
                     /gene="ruvB"
                     /locus_tag="P5541_04040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRV71182.1"
                     /translation="MNPDLHERESNNSFLNREEETHGLRPLRLCDFIGQDNLRKKLAI
                     YIKAASNRNEPLDHCLFYGPPGLGKTTLAGIIANEMEGQIRVTSGPALERAGDLAAIL
                     SNLQPKDVLFIDEIHRLSPAVEEVLYPGMEDYSLDIIVGKGPLARNIRLQLPPFTLVG
                     ATTRLGLLTSPLRARFGIVEQLESYSTKDLAAIISRGSRLLDIEVSEEAANAIAGSSR
                     GTPRVALRLLRRVRDVAEVRNIPNIDASLARAALCMLGIDSIGMDDRDRRYLHALSVL
                     FGGGPVGLSTLAAALNEESQTIEDIYEPFLIQNGFIERTPRGRKAMVKAFDFLGIEYR
                     DENTSEQRLLSLEENGSEEDGEE"
     gene            complement(16148..16750)
                     /gene="ruvA"
                     /locus_tag="P5541_04045"
     CDS             complement(16148..16750)
                     /gene="ruvA"
                     /locus_tag="P5541_04045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRV71183.1"
                     /translation="MIDSLQGKVTRIDTEGIAIEAGGIGFYLMCSRGVFMEAREGTET
                     RVLASLQVSDVGPSLFGFIDETERSLFNLLLKVRGVGSRLAITILRNLELKTVVTAIS
                     RDDTTALCQVPGVGKKTAERLCFELKQGLERSGLEGFASCGPTENSNVAQFVIDAMES
                     LGFSRSEARTAVTRVVEDRPSVPESPEELLREALDKIRRS"
     gene            complement(16747..17256)
                     /locus_tag="P5541_04050"
     CDS             complement(16747..17256)
                     /locus_tag="P5541_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRV71184.1"
                     /translation="MERVCFGVDPGIGRLGYGVVVQSGAIFRAREFGCLTTSPGKPPE
                     QRILKIYNELSLLVEKNMPDIFSVERLFFGKNRTTAESVFQVRGAVLLLAAKAGIPLV
                     QPKPSEVKMAVCGNGRAGKGQVEGMISRILNMERTIGQDDAADALAIALTGLAMYNYS
                     NLTKSGDGY"
     gene            complement(17264..18016)
                     /locus_tag="P5541_04055"
     CDS             complement(17264..18016)
                     /locus_tag="P5541_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV71185.1"
                     /translation="MSGHSKWANIKHRKSAQDAKRGKAFQKYIRAVMVASKTGGPDPS
                     MNMSLKAAIERARGANVPVDTIEKAVKKGSGDMDGASYDEIMYEGYGNGGVAVLVETL
                     TDNKNRTASEIRFLFSRSGGSLGEAGCVSWIFQRKGAIRVTGNGLDEDQLMGICLDAG
                     AEDLEDNGEGFTVTCDPAAMTEVRDQLTNAGYVIESTGLEMIPKTTVELKNRPEAEKL
                     LKFLETLEEHDDVQNVYANFDIPDEIMEEIVG"
     gene            complement(18034..18780)
                     /gene="nadE"
                     /locus_tag="P5541_04060"
     CDS             complement(18034..18780)
                     /gene="nadE"
                     /locus_tag="P5541_04060"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRV71186.1"
                     /translation="MPIYRDPEFLIEKISAWVFQKIKESGRAGGIVGLSGGIDSAVVA
                     ALLSRVCGKKMAVVLMPCHSNDIDESDAMLVVEKFDLPWIRVDLSATYDALLRAMPAE
                     TSSVNGVHLSNIKPRLRMTTLYCLGQQKKLLVCGTGNRAEIHTGYFTKHGDSGVDILP
                     LGDLLKGEVIGMARKLGVPATVIGKPPSAGLWAGQTDEDELGMTYQQIDRYLATKESD
                     DATAKKIDDAFRRSSHKREIPPICLIDRQI"
     gene            complement(18852..19679)
                     /locus_tag="P5541_04065"
     CDS             complement(18852..19679)
                     /locus_tag="P5541_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV71187.1"
                     /translation="MKKIRVHIIQSDITWGNPAANIKKMSETFNGLPDGPSVVVMPEL
                     WTCSYDNPSLQYHSEQSPRALDMLQGVARNKGLYIVAGSLPWKDQPDGPIYNRCYVIG
                     DGGEVTGFYDKAHLFPLLDEPLFFRPGERPFLFEIYGVTASVAICFDIRFPEFVRSIA
                     LSGAQMIFVPAEWPSTRIDHWITLLRARAIENQVFIVACNRCGKGGNEIYGGNSLLAA
                     PDGNIVFQAPGSGECVNTIDTDISNLERIRKKFPFTAGRNPRLYTPVTSMGEAEKQE"
     gene            19717..20124
                     /gene="nikR"
                     /locus_tag="P5541_04070"
     CDS             19717..20124
                     /gene="nikR"
                     /locus_tag="P5541_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRV71188.1"
                     /translation="MEKLARFSVSLPSDLLERFDRWVEVRGNKTRSDALRQLIRRYIS
                     ESCWEDGDQIVFGTVTLFYDHHSHDSAQEITSLQHEFGDIIICSTHIHVDHHNCFESV
                     VFRGKSSRVKKFAKDLSGLKDVSSSEPVISTIP"
     gene            complement(20397..21344)
                     /gene="argF"
                     /locus_tag="P5541_04075"
     CDS             complement(20397..21344)
                     /gene="argF"
                     /locus_tag="P5541_04075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRV71189.1"
                     /translation="MPLNLKGQSFLTLKDFSREEIQYLLDLSVGLKEKKRSGVRGDAL
                     AGKNVALIFEKPSTRTRCAFTVACIDEGAHPEYLGKNDIQLGHKESVKDTARVLGRMF
                     DGIQFRGFKQSVVEDLARYSGVPVWNGLTDMYHPTQILADFLTIQENFGRLRGLNLVY
                     SGDGRNNMANSLMIGSAKMGVNFTIAAPAGLFPEKELSEECMAIASTTGAKIAFIEDP
                     REAVKNADAIYTDVWASMGEEDKMAERAALLKPYQVNMDLMKATGNPECIFLHCLPAV
                     KGNEVTEDVFESFSSKVFDEAENRLHTIKAVMVATAGNI"
     gene            complement(21381..22634)
                     /locus_tag="P5541_04080"
     CDS             complement(21381..22634)
                     /locus_tag="P5541_04080"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HRV71190.1"
                     /translation="MTEEERSFSVSSEVGKLKRVMLHRPGKELERLTIDNKDSLLFDD
                     ILWVEEAQKEHDSFADLLRDNGVEVVYFRNCLSHVLRDEEVRDSLLDEALALEAIDIP
                     LASALKSVLKEETPSDLAEILIAGLTKEEALEFMPPCRSLVLQAMSDRDFLIRPLPNL
                     YFQRDPFSFVNNGVIISVMNFEARRKEPLYAKYIFRHHPYFKDIEILFGEDYRDTYPY
                     NIEGGDILVLSDSIVAIGISQRTAAGAIQIIGKRLAEKAGVRKVLAVDIPKTRSAMHL
                     DTVFTMVDRDAFTIYPEVRDFIRVWELDYDEIGKVTSIEEHKSLGGCLKKALNLEEVR
                     FIETGGGDPVAAARDQWNDGTNTLAIAPGVVVTYRRNVVSNRVLQENGIKVFEIKGAE
                     LGRGRGGPRCMSMPMMRDPAVFSIK"
     gene            complement(22730..23149)
                     /gene="speD"
                     /locus_tag="P5541_04085"
     CDS             complement(22730..23149)
                     /gene="speD"
                     /locus_tag="P5541_04085"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRV71191.1"
                     /translation="MKNGESLGRHILVEAYDCDREALDNIGELQEAMETAAEKAGATV
                     VESAFRKFQPHGISGVLVISESHLTIHTWPEFGYAAIDLFTCGCNADPWKAFEHLSSY
                     LKSSRTTSIEIQRGCISTGSEGAINSILPKESAMALD"
     gene            complement(23146..24000)
                     /gene="speB"
                     /locus_tag="P5541_04090"
     CDS             complement(23146..24000)
                     /gene="speB"
                     /locus_tag="P5541_04090"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRV71192.1"
                     /translation="MKRFLESRRTHSGVKWILQGLPLDMTVTRRHGTGQAPSVIREES
                     HGLETYSFPLDKDLSEIDFCDIGDLNLPGDDLCRSLFIIESVSSRFFDMGKRVASFGG
                     EHLVTLPVVKAAVKTFGDLVVIHMDAHADLREKYHGMKYNHATVMRWIAEECLVSPGS
                     LYQFCIRSGTREEFEWGMANTSFYPGELLRPLGKCLERINEKPVYITIDIDSVEPAEA
                     PGTGTPEPCGTPAREIFEALYEMKGLNVVGFDIVEVSPPHDVNNVTSVLAAKMARECL
                     IMWGGDNC"
     gene            complement(23997..24851)
                     /gene="speE"
                     /locus_tag="P5541_04095"
     CDS             complement(23997..24851)
                     /gene="speE"
                     /locus_tag="P5541_04095"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRV71193.1"
                     /translation="MDKRITDLWFSEYQTPDLCLGLRITGILANRKTPYQHLLVADTE
                     EYGRLMALDGAIQVTERDEFAYHEMLSHVALSSHPSPKNVLIVGGGDGGTAREAAKHC
                     SVEEIVLVDIDQEVIDASREFFPSLRVGLDHPKVTVKSMDALDYIRERRGQFDVVLVD
                     STDPVDFAEGLFKESFYRDVYNSLNDDGFMVAQTESPFAEPKILSDAVSEMKKVFPLV
                     KVFWGAMPTYPTGVWTYSIASKKHDPEGIRSDIPVDTRYYSKEIHRAAFVLPPFLEDI
                     IAGRYQES"
     gene            complement(24865..25341)
                     /locus_tag="P5541_04100"
     CDS             complement(24865..25341)
                     /locus_tag="P5541_04100"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRV71194.1"
                     /translation="MLPVPELFTLVVGRGEGKKKLTAFDSALLDAGVGNMNLLRVSSV
                     LPPHCTFRESLSMPFGSLLPIAYGSITSDVPGETISASIGVGIPQDPESFGMIFEFSG
                     FCPGSEAAARVEEMVREAFDMRSLALKEVKVKAVDHVVKECGTVFAGCPLFFPTKF"
     gene            complement(25477..26538)
                     /gene="ispG"
                     /locus_tag="P5541_04105"
     CDS             complement(25477..26538)
                     /gene="ispG"
                     /locus_tag="P5541_04105"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRV71195.1"
                     /translation="MSARSVTIGALGIGGSFPVRVESMLRTPLGRTGECLEEILNLHE
                     NGCELARVAFPEVLLADSLSFLLRESPMEIMADIHFDHKLAMKAIEVGCRSIRLNPGN
                     MASKSGLAELCKMIIENGTVVRVGANGGSLSKAQIAKAGGNRVSALLAAVDDQIRALE
                     GHGVGEIIMSLKSSSMNETLKANTLLAERFDYPVHAGLTEAGPGERGIIKSSVALGSL
                     LGSGIGDTIRISLTGPAIDEVRVAKRIIQSLGLRRFEPDLISCPACGRRRASVPELVN
                     LIEPLLEMLEPQLTVAVMGCEVNGPQEASGADIGIAGTPSGLMLFVKGKPLGRCSLDD
                     LEIRFLDVCKSQGFLKPGK"
     gene            complement(26572..>26876)
                     /locus_tag="P5541_04110"
     CDS             complement(26572..>26876)
                     /locus_tag="P5541_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV71196.1"
                     /translation="GRGQVDVTGPVGIASIAGQAAREGLWTFLAFLSMINLHLGILNL
                     LPFPALDGGRILIIGGEIVTGRKLPEKWENLVHLAGFALLILLLLFVTWKDLIRLF"
BASE COUNT         6535 a         6975 c         6349 g         7017 t
ORIGIN      
        1 atgctttccc cccgactctc aagaagatct ctgtcaaggt cgaaaaaata atcgttatgt
       61 tccccgaaaa tttcaagtgg gaccgggaag tgaagttcag ggtcggggtc ctccgatctg
      121 ggtaaatagt cctttcctga accatcgggg agatcgtttt ccggaattgc gatctttccc
      181 ggggtctttc cggtttgcgg ttttgtggtg gcctgttttt cggaaacggc ggagtttgac
      241 aaagaagcct ggttatcggc atttcttccc gattctgaaa aaatccctgt aaggaatgaa
      301 aaagttctgc gggaaagtac cccgtaaagc cccatggcaa gagatatgcc cgcaaaaccc
      361 gtaaatacgg tgccaagagg tcctatcttg ttaaaaagtg tcattgctat agcatttccc
      421 aacgtgcctg gttgaaggag atttgctgtt gaggagatgc cgctaagacg ataaagacca
      481 aggaaaaggg agcagcatat aaaaagaacg gctgtgccaa aggtctgcct tgcaaggctc
      541 gggatcctga gacctgagag ccaggctaaa gaaagccagg cggcgaaaag aagcagaatg
      601 attatggcag atcccggaac ctgaagtgcc catgaatgga taacatcccc cagtgatccg
      661 gtacggaaga gaaagaccgt cgcgagaatg taaaggtcaa caaggagcag ggcaaaaaca
      721 gaagcgatca agagaggtga ccttggcctg tcccccttgc tgattgattt tttgttcttt
      781 tcggaagacg ggttctttgc ttttcttcta gttaccttca gtgtcgcttc ctcctacagt
      841 catttccctt ataaggagcg agggctgacc atctgttaca gggacattct ggcccgactt
      901 tccacacatt cccgattcga agtgaagatc gtttccgacc gcctcgatat tcatgaggac
      961 ctgtgggccg cttcctgtca gaatggcatt ttttacgggc cataccgggc gcccgttttt
     1021 tataagataa ccctcggtaa cgtgaaaaac aaaatcaccg gaagtcgggt ttacctctcc
     1081 cccacccatt ttttttacaa gaagaccttt ttttatcctt gcaaccagag attcgggttc
     1141 ctctgtaccg ttcattataa atgtatttga cattctggga acaggagaga ccttgtagga
     1201 agatcttctc ccgtttcctg tcctggggaa atcaccctgg atcgaggaat ggatatctgt
     1261 cagaaaagac tcaaggatac cgtcacgtac aaggatagtc cttttcgaag gggtgccttc
     1321 tccatcaaag ggataacttc cgaaaaggtc ctgtatcatc ggatcatcgg cgaggctgac
     1381 caggggagat gcgacttgct tccccattga gtccctgaaa accgaaaagt ccttgaggat
     1441 aatatccgct tcaaggctgt gtccgcaagc ttcgtgtatc atagtgcctc ctgccatgcc
     1501 cgatatgaca actggcatcg atccagcggg acagggaaga gcctcagaca ttagaatggc
     1561 tcttctgaga gcctcagttg ctactgattc caggtcaaca acatcaagaa actcccctgc
     1621 ggatatactg agggccctga cctcccggcc cgtaaacaag gcaccgtttt tttccaatat
     1681 gaccgaaaca gtgaatcgtg tctttgagga cgagaatctg caaagagggt tttccgtacc
     1741 ggcaatagct atttctgatg acgcactgcc ccatcccaga gtaacctgtt tgatcgagtt
     1801 ggattccttg cgaattgcct tatcaatatc ccggatcctt tcgagggggt catcgtccgg
     1861 aatttcggga tcaggcagaa gcagatcgtc ccgggaagca ttcctggaag ttcttgaaaa
     1921 tcccgccctt tcagagctgc cagatatgca atccagagcc ctgttgaagg tcgcacccgt
     1981 agaatgggta aaaaatgtac ggtcccccat gacgatcctg atgccggtgc catctgtcct
     2041 ggaagccgat atctcctcga atttaccgtt gtcaaaaaga gcgtatcttc cgtgactcct
     2101 ctggataaaa gcgtccgaaa aggttattcc ggggatttta agagtttcca tgatgtcgaa
     2161 aagtctgtcc ataggtttca ctcctcttaa ctaaccgctc tattccgggg tgttcatcaa
     2221 tgaatcaaat tcgctccgcg cccttttgat ggccccaagg tgttcattat aggaaaccga
     2281 gaaaaaatga cttccgtctg gcctggcaac ataatacatg tatggtacaa aatccggatt
     2341 cagggctgca tcccatgagg atgcagatgg gacgcatatc ggcgccggcg ggtatccagg
     2401 ataaaggtat gtattgtaag gggaatcgat ctcgagatgg gagtaaagga cccttttaag
     2461 cgatagaccc ctgagggccc atgcgtagac cacggtagcg tctatctgga gtttcatccc
     2521 cgatctttcc ctgttgagta taactccggc aataattttc ttttctttct cttgccgggc
     2581 ctctttctcg atcatagaag caaggatcgc tgtttttaga agatttttgc tatccagggc
     2641 aagctcttcc tcaattctgg atcctaccct tttccaccat gcaaatgaag cgcttttgat
     2701 cagttcttca gccgcccaga ccgtgggtga cacgctgtag gtgtccggaa gaagaaaagc
     2761 caatcgggac aatggatccg ccggaaggaa tcttctgact tcttcaggaa aaagggagtc
     2821 tttttggata gcgacatgaa tcgtgtcacc gattttctgc gctatttccg gtagcgactc
     2881 tccggggata atggtcatcg atgaaatctc cggttccgac cgctctatct gccgggcgat
     2941 ctcccagggg gaaccgggac ggatgcggta gattccgggc ctgatagaac gatcaattcc
     3001 aagcttgaaa aaaaagcggg caaggtcaga agggctgtca gtaagccctt cccttttggc
     3061 aagacgggca atatcgaggg cacccattcc gggttctact acaaattcct gaggctcccc
     3121 cctgccgggt tcaatgatga ccgaaagcct ttccggaacg atccatgtta gggaaacgta
     3181 aaaagccgcc gtaacaaaaa ataacacaaa attctgaata aaaagggcca gacgtgccca
     3241 tcctgacctg gaatatgtca tgacggatca agggaagaag cgacccggac aagcactttg
     3301 ctaaccgtaa tttccactga taccagttct ctttcaatga tggcgttcga agttttccga
     3361 taatcagggt catctatgga ggacaaattg acctgggcat ttaaggccgc gccttttgcg
     3421 gcggaacctg ccataagagc agaaactccg gcatcggtta tggcgttggg gttacccatt
     3481 tctgccgctg tcaggcaaag ctcggaaagt tcacgtgaaa ggcagaggat ccgcagggga
     3541 acttcagtgg acatcttaag cgccccctgc atggcttcct ttcttgcgga cttctcggta
     3601 tcggaatttt tcggcatcga gagagctttc atgaaagagg tgaaagcttt tgtgtcttcc
     3661 gaggcctgtt ccaaaaaatc ctgagcaagt tttattgcgc tttctctgac tcttgtcatg
     3721 gcctcttcgc tttcagcgta tttctcccgt ccgacagtca ggtttgctac cattctcgca
     3781 agagatgcac ccagggctcc cgaaaccgcg gccacagaac caccgcccgg tgccgggctg
     3841 gatgaaccga gttcctcgag aaaatccgtg agcttcattt catggaaatt caacaagttt
     3901 ctctcctcca ctcgcacaac tttgaaacga cgggctctcc ttttttgtag accgaaagca
     3961 ccggatttac accactattg tatgccagaa tggcaggagt ttcaccgtca agaataacaa
     4021 ggtctgcaaa ttttccaggt tcaaggctcc cggttcttga atccatggcg atcgcctttg
     4081 cggcattaag agtgcagccc atcagggcct cctctgtggt catggccata ttcattaccg
     4141 caagactgaa tacgaagggc atggaatggg tgtaagagga accggggttg cagtccgttg
     4201 aaagagctac ggtaaggccc agatccatca ttcttctggc gttggcgtat ggttttttaa
     4261 ggctgaaagc tgttgcggga aggagtacgg ctattactcc agcggctgac atggcctcca
     4321 gatttttctc gcttgcagca aggagatgtt ccgccgaaat ggctcctatc tcggcagcca
     4381 gcgctgcccc accgatgtca ttaacctcgt cagcgtggat cttggctccc atgccgtggt
     4441 tggaacctgc ttcaaggatt cttcggctct gggggatagt aaagacacct ttttcgcaga
     4501 aaacatcaca gaaagagggg gaacactctt tggcaacagc tgggatcatc tcttttacga
     4561 taaggtctac gtatccatcg gggtcatctc tgaactcctg tgggactgcg tgtgctccca
     4621 taaaggtcga tacaatatcc ggcggtgtct cccttgaagc cctggctatg acattcagca
     4681 tcctgatctc ggaaggggta tccagcccat agccgctctt gatctctatc gtggttgtcc
     4741 cgtggcgcaa ggccgtttca atattttttc tcgtaacccg gaatagttct tcatcgctgg
     4801 attcccttac gttttttact gaagagagta ttccgcctcc tgccttcagg atgtccaggt
     4861 aaggggtccc ctcaaggcgc atggcaaatt cctgctccct taatgacgcg aaacacatat
     4921 gagtgtgagg atccacgaaa ccggggatca cacacttccc catgcagtct atctgctggt
     4981 ctgtctccgc cgaaagaggg tgtgcaagaa catcctcaat attacctgcc tcgattatca
     5041 ggccgtccct tacaaggaga gctccccttt cgtaaaagac gatccccgaa ccagatgctc
     5101 catcgttgat gggagtaaat atcttgccgt tcatgaaaag cttcgcgttc attattcttc
     5161 tccgatcatt tcaagaagtt tgagctcaat gatctgtgag gggtcaaaat ccacggtctg
     5221 aagatacctc accgcgctct catacattgc tgaaaccggt accatcccat aaagttctgt
     5281 ctcaaggacc gggattcccc acctggcggc ctcattcctg accatatcca gaacccggta
     5341 aagcggattt ttctcgtgat ctacgatatt gatacttacc tgtacgaggc ctctctcttc
     5401 gagagggagg ccgatcgcct tgacatgggc cagtccgccc ccggaagccc tgatgctttt
     5461 ggcaatactc tttgcgatat cgattttgtc agttccaagg ttgacattaa aggcaacaag
     5521 aaattttctt gcgcctacaa cagtagcgcc tgctgtggga tgaagagatg gttcccccac
     5581 gtcaggatgc cttccaggct cctttacatt gttaatgagg gcctcgtatt gcccctttcg
     5641 aatgttctcc aggtttttcc tttcggggct gagggcagcc tcctcgtaat agtaaacagg
     5701 gatgcccgtc gctgaattga gcctttttcc gaaagaatga gcgatctgaa cgcactcttc
     5761 cattgtggat gtgccaagag gcgtgaaggg gacaacatcg atggcgccta tccggggatg
     5821 gcctccccta tgaacattca tgtctatatg ggaaacggct tcagcggcag ctttgagaag
     5881 ggcttcctgg agaggctccg gttcacccgc aaggctcacg accagcctgt tgtggtcctc
     5941 atccgccctc gcgtccagaa aagtaaaacc ccgacctccg cgaaagggag aaagaatttt
     6001 ttctattact tccttacgcc gtccctcgct gaaattaggc acgcattcaa ctatcttcat
     6061 gacgggatcc cccttttacg gctgcttaac tatcaggttc agaagcattt cttcatttgc
     6121 cagataagga agggttatgg aatcatcggt actgttgtta tgttcaacaa ttgtgtcgat
     6181 agagttgggg ttccttgccc aggagcgtcg ggcaactcca ccaataacgt cccaggttat
     6241 tgattttctt attacttcat ctactctttc gctgccatcc aggaccagac cgaacccgcc
     6301 gtttatggct tttcctatcc caaccccacc accgttatga agagccacaa ggctcatccc
     6361 tctggccgcg ttgccagcaa agcactgaac cgccatatcg gccatcacgt tgcttccgtc
     6421 cctgatgttg gcggtttccc tgaagggaga atcagtccct cccgtatcgt ggtggtcccg
     6481 gccaagcatg acgggaccga tatctccatc acggatcatt ttgttgaaac ggagagcgat
     6541 ctttactctt ccaacggcat cctggtaaag gatccttgcc tgtgtcccga ccaccatccg
     6601 gttctttccg gcatccctga cccatatgta gttatccatg tcctggggac gtctgtccct
     6661 gtcaatgcat tccatggcag cgttgtcggt tttctccaga tcctccggtt ttccactgag
     6721 gcagacccac cggaatggtc cgtagccaaa atcaaaaagc tccggtccca tgatgtcttc
     6781 aacgtaggac gggaatatga aaccatccct ggtatccttg ccgttaattg aaatttcagt
     6841 gtatccggca tcgaatatgg ccttcatgaa ggagtttccg taatcaaaga agtatgtccc
     6901 tttattgaca agtttccgga tcagatcaaa atgttttccc agactgctgt tgaccagttc
     6961 cctgaacttg tttcgatctt tttgaagcat ttcggtcctt tcggcgaagt caagttgcca
     7021 tgggcagtac cctccttcgt agggggcgtg acaggaagtc tggtctgaga gcaggtctat
     7081 tggaatattg tgatttaacg cgtattcgag caggtcaact atgtttccgt gataggctat
     7141 tgatattccc tttttttctt ccctgtattt gtttgcgata ttgaaaactt ccggcagatc
     7201 tgaagagaca acggaaaccc agccctgatc gtgacgtgtt ttgatccttg acatatccac
     7261 ttcggcaatt attccgacgc aacccgcgat ctcggcggat ttgggctgtg caccgctcat
     7321 tccacccagc ccggaagaga caaagacaac tccctcaagg ccgctttcgc ttcccagacc
     7381 cagctttctg ccagcgttca gaatcgtatt gaaggttccg tggacgatcc cctggggacc
     7441 aatatacatc cagccaccag ccgtcatctg gccgtaattc gccacaccca tctgttcggc
     7501 aatttcccag tcgtcgggat tgtcgaacat tccgaccatc agggaatttg tgataacaac
     7561 ccttggggca cccggtgaag aagcaaagag gccaagaggg tggccagatt cgacgacaag
     7621 ggtagtttcc tgggtaagat tctccagata tttttttatt agacggtact gcatccagtt
     7681 ctgacaaacg cttcctgttt ctccgtaagt aaccagttcg taaggataca aggcaacctc
     7741 ggtgtccagg ttgttgtcta tcattacctg gaaagccttt ccttcaaggc atttcccttt
     7801 gtaaaaatca acgggcatac cctttatgga cccgtcaggt cgataccggt acccgtaaat
     7861 cctccctctc gtcctgagtt cttcaaggaa ctcgggagcg acagtgtcat gatgctccgg
     7921 gggaatatat ctgagggcat ttttcagagc aacaagagtc tggtcagctg tgagcctgta
     7981 ccccctgtcc ggggctcttc tgatggaagg atcaaatgca ggcatctcgg gaagatccgg
     8041 gtcaagcctt atcttcattg ccttttctac aatattattg tttttcatta taatatcgcc
     8101 tccgtcaagg ttgatttatt caagaatacc tatagcatcc tcgactcccc tgaggtagga
     8161 gccatcttcg atcctctgta tcactttcgt gatcatgggg tgaaggaagc tatcctcatt
     8221 caggtgggga acctccgtcc ttacaagttg atggattacc gcagtgcctg cccccggttt
     8281 caggggactt gaaaaatcaa caccctgcga tgcaaggaga agttcgatcg ccaggatgtt
     8341 ttttacatta tcgaggatct cccttccctt tctggcagag tacattccca ttgaaacatg
     8401 atcctcctgg ttagccgagg ttggaatcga atcaacacag gaagggtgcg acaagacctt
     8461 gttttcggaa acaagtgcgg cagaagcgta ctgtgggatc atgaatccgc tgtttattcc
     8521 gctattggct acaagaaacg gaggaagtcc cgaaagcttt ccgtctacca gcctggccga
     8581 ccttctttcg gatatacttc cgatctcagc catagcaatt ccaaaaaaat ccatcgcaag
     8641 ggcaacgggt tgcccgtgga aattacctcc acttatggct tctccctcag cagggaatat
     8701 caggggatta tcggttacgg agttgatctc tatctggatt ttttcatgga cataatctat
     8761 cccatcacgg cttgcaccat gaacctgggg aatgcatctt aacgaatagg catcctggac
     8821 tctttctttc ttgtgttttt cgacgatctg gcttccttct atgagtcttc tgatattccg
     8881 ggcgacccgg atctgaccct gatgaggtct aagttcgtgg gtcctggaat caaaagcgta
     8941 ggggactccc ctgagggctt caagggatag tgccgaaatg atgtcggcgg ttttcaaaag
     9001 cgaaagggcc tcccaggttg ccattgctgc aactcctgtc attgctgcag taccattatt
     9061 cagtgcgaga ccttccttgg gcccaagtct gaccggttcc aggccgttaa tcctcagagc
     9121 ttccgatgct gagatctgct ttcctttgta aaacacatca ccaataccaa gaagtgctat
     9181 cgcaaggtgt gaaagggggc aaaggtctcc gctggcgcca acagagccct tgcatggtat
     9241 caccggatgg atacccaggt taagaagatc gacgagcctt gaaagagtct tcatgcttat
     9301 tccggagaac cccctgatga gggaattgat ccgcagaagc attattgccc tgacgatatg
     9361 ttctggaaag ggatccccga ctccgcacgc atggcttacc aggaggtttt cctgcagttt
     9421 ttcactttta tccgccggta ttacctggct ggcaagatct ccgaacccgg tcgttattcc
     9481 gtatatcgtt ttgccctcat cgacccagga gcgcaccatt ctggagcctc tttcaacaaa
     9541 ggctacagcc gaactgtcca gggcgatttt tttgttcatt ctcgcaacct ggacgatatc
     9601 ctccagcatc aaggaatgac cgttcaattc tagtatcgag gataccattg gcatcgaccc
     9661 ccaaaatagg attactttct atctgaaaga ctatctcgaa aatcatctcg tgacaatacc
     9721 caggggggtt ttacagaatg gacaattccc ttcaggtgtg gcaattgttt ccacagagcg
     9781 gccgtaatcc ctggtaataa ccaatttccc gcaggagggg caaaatgtat catttttttc
     9841 gccagtgttt cctgtgtaga cgaaattcag gtatctcgat gcgatctctt tccttttccg
     9901 tatctcctgt agtaaagtcg gtggctcttg ccattgccat gaaggaaagt atcttgaaat
     9961 gtgaaggggg atattgtttg aaagcccgct tatccacctt gtcagggaaa ggaattcctc
    10021 aagagagtcg ttgatcccgg gaacaacaag gtgtgtaatt tccaggtgga tacctctttc
    10081 caccgctctt tttatccaat gaagtacagg gccgaccgaa ccgccgagct tttcgtaggt
    10141 tcggggataa aaacctttca gatcaatatt catggcccct gtgatatcaa gaagttcctc
    10201 aagggggtct ggggaaataa aaccgtttgt tacaagaaca atatctgctc catatttttt
    10261 caaaggttcc gagatatcca aaataaattc aaaccagacc gaaggttcgt tataggtaaa
    10321 tgctaccgaa gactgtccag aatcacggac aagggaagaa aaaccattcg gagaaaggta
    10381 tttcaatcca gtcggtgttt cggatctcgc gagatgggcg ttctggcaga agggacagaa
    10441 aagattgcaa ccgaaagtgc cgaccgaaaa aatctccgtt cccggcttcc agtggaaaag
    10501 aggcttcttc tctaccgggt ctacggatgc gcaaaagtaa ccgtaattga gagtgaaaag
    10561 ttcccctccc ctgttcagcc ttgtcaggca gttccctttt tcccctggta gaataaggca
    10621 acccctggga catagaagac accttgtatt tccattattt tcctttttcc agtattttgc
    10681 cctggtttct gtcttttcgg tcattctgaa aacctcgaaa cgcgaaaccg gcttatcctg
    10741 accccctctg cagaaccaat tccagccttg gaaagggcta ttcccaactg tttttcaaca
    10801 gtttcgactc catcgaggtc cgggagaagt actccccttc tggagccttt ctcaacgatc
    10861 accccgaaaa ctttcggatc aaggagagac agatcagtga tcggttccgg ttcatccaga
    10921 acatccacgg agatccggca gtttaacagt tcattccatg ccacaggcat aaaacgaggg
    10981 tctttacagg ccgccgagta ggcatttgaa acaatttcct caccaatgct gggaaaaact
    11041 ggaacaacag taccaatgca ccctctcagt tcgccgccaa cggtcttgat ggaaacaaaa
    11101 catgcttttc tttcttcaag ggacttatca ctgaagaggg gcatttcttc aaggtgatca
    11161 ttagggtctt tgccaaggga ggccatctct attgcttttc ttgcaagtat caagtgatat
    11221 ttccctacgt tttccgcagg tctttccggt ttccagagag cggtagcgta tccaactcca
    11281 aaaggagcct cataggataa cagttcgatt tcttctccaa gagaaatacc tataagggcg
    11341 agaaccgagc gaaggccaca ttcgccgccg ttcaatattg tccttacggt aagtgaaaaa
    11401 accggttctg gtgaagcaga tactattgat tcctggatct ttctgtcaag gatctctccg
    11461 tcggggtgga atccggcagg ggaatccttt ttgagtctgt gtgagagatc cccgcttgcc
    11521 agaagcagcc agtctgtgct ggagttatac cgccgaagat ggatcccgaa tttatatgcc
    11581 gaggagaaat caagcaacag gggattgaag atcaacaagg gagggatctt tccgaatatc
    11641 atctcgaaaa aaagaagagg aacaacggaa gcataatcaa gggtaaagac atctggtttt
    11701 tgctctgcta tcgaaaacat ttgatttatg tgctccagaa gggaagaagc ctcttttccc
    11761 acacctgtcg catcaacact ctggtttccg gctccgaaac cggagagaga acctttgaaa
    11821 cgatagctgt tgacaacgca gggtttcaag ccggtttgag cgtgggggtc cagaagaagg
    11881 atcgcctcgg gggtatgccc tttaagtttg gttttcatgg aaacaatggc ttcaagtgtt
    11941 tttcccggcc ttacacccga ccttgacgca acttccggca ccaggatggg agcgtgaggc
    12001 agaagcagac cggctgacca cattttttaa tcctcccggg cacgaatttt gctatatata
    12061 ttgtattatg acagtgtcct gtagcatttt tcaatattct gaaattattg aagccgaggt
    12121 gtaatcccca tggggccgcc tgaaggcaac cagaaagaat ccagagttct tgacctgatt
    12181 ggcagactcg aggagttaat tgaagttttt caattaactg aaagtgattt ttccgaattg
    12241 agggacatac cctttccgaa aaggaagttg tctgctattg ccggtgttct tgcccttcct
    12301 ctttcacagg cagcgagaat actcctgatc tcctccgttc ttcagagccg gggagatgaa
    12361 cttaactgcc gtttcaacga actggcttcc gtttccggtc tgtcagaaag aacggcttac
    12421 agggctctca aggagatcaa ggaatcgggc cttttcgaca ttaaggcagt tccggggacg
    12481 ggtgtaacca tttactcccg ttggctctgg agcaaaccgg gggaaagggc gctcaggata
    12541 cttggtgaaa acagggatat ccaggatcag agtatcagca tggagtcacc aaaggaataa
    12601 aaccggtatc ccattgagac agcttccctg taggctttca tggtcagatc aaggcccgcg
    12661 aaagcggcca ccagcataag aagggtgctt tttggaaggt gaaaattggt taccatcaca
    12721 tcgaccatac gaaacctgta ccccggataa aaaaaagcat cagtgctgaa gggtccggcc
    12781 tccagttctc cgttatcaat ggttctgctt tcgagtgctc ttacagcagt ggttccaacg
    12841 gcgacgaccc ttcctcccct cttccgggta ttcattattt ctgtggcggt ttcttccgat
    12901 ataaatccct cttcaatgtg catggcatga tcccttatat cagaggtctt gactggtctg
    12961 aatgttccca gacctacgtc aagagttatg tttttttgaa ggactccgat gcttttgatc
    13021 ttttccagta atggacgggt aaaatggagt cccgcggtgg gagcggcaac agaccctgaa
    13081 ttccttgaaa atacggtctg gtacctttcg gggtcgatgg cgctattttt tatgtatggg
    13141 ggtagcggta tttctccgtt cttttcgata aatgacatta cgtccagatc atcgggaaac
    13201 cggcatatcc ttgtcccatc gttaccctca cccagaactg taacggggat atcacctttc
    13261 agtattaccc gtctgccagg tttgaccctt ttcccggggc ggataagggc ttcccactcc
    13321 accgccacgc tattctgcat agcaaccggt ttaagcagga agacttctat cctggattcc
    13381 ccgccctctt ttttcccgat caatctggca cgaatcaccc tggtatcgtt aaggatcatg
    13441 atgtcgcctt ttcgaagaca ggaggtaata tcccggaagt atcgatggta catttcacct
    13501 gatgaacgat ctataaccag aagtcgggag gagtccctgg gctcgacagg atcctgtgcg
    13561 ataagttctt ccggaaggaa gtaatcgtaa ctggcggagt tgaaatagcg tggatcattc
    13621 ccgggcgatt tgatttttcc cattcagttt gatatctttt ccagtctggt gcccggaaaa
    13681 taatgttgca atattttttc aaaaccccaa cctgcttcag aaaggctttt ggctccccac
    13741 tgggaaagtc ctacgccgtg accccatccc ctgccagtga aaacaacggt atttccagag
    13801 gacctcgatg ggggctgttg tggattcggt aaggaagact catttgagca tccgctttgt
    13861 ctttccagag caactctgag atagtggcct ctcttttccg gttttatgag catgtccata
    13921 agttcctcgg agttgaaaac cccgtttttt gtgagggaga tcaaaagttg ctcatcccgc
    13981 ggagaaaggg ggatgttatc ctgatcttga gcggcagaag aaggggtgga gttttggggt
    14041 acgtttgttt tcaggttcgg gacagcttcc cctatcctga aaaaagtgct ttttatcctg
    14101 tccgggccca tgaaagtcct gaactgactt gatttcagcg tcacacttcc agaactgccg
    14161 gataccgtga tcatttccgc cctgccagcc atgtccaccg atgcgatctc aatggatgaa
    14221 aggttcccga cattgactcc ccttgcggaa agaacccgct gtatttcaga aaggggtatc
    14281 ctgacctgcc agttggaata gggggactcg taggcaattg gttccggttt tcccataagg
    14341 tagggaatga ctgaactcca tacagtggat acatcagcgg tatgcccccc actgtcggag
    14401 tggtagaaag tcgccgcaat ggtaccctgg tacttcagga ccattcccct ggtcaaatct
    14461 accgctctgg taaggatcgg gtcttcggca ttgacgcccc tgtaaacctg gcaatggctt
    14521 gttgcgcaaa tatcgtaccc ggcagacccg tgcttgttcc tgtttctcag agcataggtc
    14581 ctcgcgatca ccgcctgtgc cttgagcact tccatgggcc aggcagggtt gacctccatt
    14641 tttagaactc ccttgaggta atcttcaacg tctataacat tgaccacggc aaaaccgcgg
    14701 ccatcctgca ccaggcgcat atatcccctg taagggcgtc cgttccattg gatcatggac
    14761 tggcttttta tcgtgactgg aagtttcatc gttttggaac ctgctactat cgttccccca
    14821 gtacctgccg aaattttaat agcagatttg aaggaggcct tttcgcccga tgcacctgaa
    14881 atattgaatg actctttccc ggcagaaaaa gaggaactgc tctctcctgt ggaaatcccc
    14941 acagatattg tcgccgcaga ggcaactgcc ggaggaaacg gagtaactgt caaggaaagg
    15001 agcagggcta tccctgcaga aagaaacaga ctaatttttc gccagcgttc attaccagta
    15061 atcattactc ttccccatcc tcttctgatc cattctcttc gagcgaaagc aatcgctgtt
    15121 cacttgtatt ttcatctctg tactctattc ccagaaaatc aaaggctttc accattgcct
    15181 tgcgtcccct tggggtcctc tcgataaacc cgttctgtat aaggaagggt tcataaatat
    15241 cctctattgt ctgggattcc tcattgaggg ctgctgccag ggtagaaagc ccaacgggtc
    15301 cccctccgaa aagtaccgaa agggcgtgaa gatatctcct gtccctgtcg tccattccga
    15361 tggaatcaat tccaagcata caaagggcag ccctggcaag gctggcgtca atattcggta
    15421 tgttcctgac ctccgcaaca tcacggactc gacggagaag acgaagggcg acccggggag
    15481 tccctctcga acttcctgca atggcgttgg cggcttcttc ggatacttca atatcaagaa
    15541 gtctggaacc tcttgatatg atcgcagcaa gatcttttgt ggaataggac tcaagttgtt
    15601 cgacaattcc gaatcgggca cgaagagggg aggtcagaag accaagcctc gttgtggccc
    15661 ccaccagagt aaagggaggc aactgcaaac ggatatttct ggcgaggggg cccttcccaa
    15721 cgatgatatc aagggagtaa tcttccattc cagggtagag gacctcttcg actgccggag
    15781 aaagccggtg tatctcgtca atgaagagga catcctttgg ttggaggttt gaaaggatcg
    15841 ccgccagatc cccggccctt tcgagggcgg gcccggacgt aaccctaatt tgcccctcca
    15901 tttcgttggc aatgatcccg gcgagagtcg tcttacccag accgggaggg ccgtaaaaca
    15961 gacaatggtc caggggctca ttccgattag atgcggcctt gatatagatt gccagtttct
    16021 tccgaaggtt atcctgtcct ataaaatcac aaagcctcaa cggtcttaac ccgtgagtct
    16081 cctcttccct gtttagaaaa gaattatttg attcccgctc atgaaggtca gggttcaatt
    16141 aaatacgtca gctccttctg attttatcaa gggcttctct aagtagttcc tctggagatt
    16201 cgggcactga gggtcggtct tccacaaccc ttgtaaccgc cgttcttgct tcggatcgtg
    16261 aaaaacccag tgattccatg gcatcaataa caaactgggc tacattggag ttttcagttg
    16321 gcccacaact ggcaaaacct tcaagacctg atctttccaa cccctgttta agctcaaaac
    16381 aaagcctttc tgcagttttt ttcccgactc ccggtacctg gcacagagcg gtagtgtcat
    16441 ccctggagat tgccgtgacc actgttttta gctccaggtt cctcaaaatg gtaatggcca
    16501 gcctggatcc cactccgcga accttgagaa gaaggttgaa tagagatctt tccgtttcat
    16561 ctatgaaacc aaaaagagat ggacccacgt cacttacctg aaggctggca agaacccttg
    16621 tttcggttcc ttcacgggct tccatgaaga ctcccctcga acacataaga taaaacccaa
    16681 ttccacccgc ctcgattgct atgccctcgg tatcgatcct ggtgactttt ccctggagac
    16741 tgtctatcaa tatccgtcac cacttttcgt caggttcgaa tagttgtaca tggcaagccc
    16801 ggtcagggca attgccagag catctgcggc atcatcctga ccaatcgttc tttccatgtt
    16861 cagaatacgt gagatcattc cttctacctg accctttccc gcccttccgt tgccgcacac
    16921 ggccatcttg acctccgatg gtttgggctg gaccaggggg atacctgctt tggcggcaag
    16981 aagaaggact gctccccgaa cctgaaaaac agattctgct gtggttctgt ttttcccgaa
    17041 gaaaagcctc tcgacggaaa aaatatccgg catgtttttc tcgacgagaa gagataattc
    17101 gttgtaaatt ttcaagatcc tctgctccgg cggtttgccg ggggaagtcg taaggcatcc
    17161 aaattctctt gccctgaaaa ttgcccctga ctgaaccaca acaccgtacc ccagcctccc
    17221 tataccgggg tctacaccga aacagaccct ttccatggcg ttatcagccg actatctctt
    17281 ccattatttc gtcgggaata tcaaagttgg cgtaaacatt ctggacgtca tcatgctcct
    17341 caagggtttc aaggaatttc agaagcttct cagcttccgg cctgttcttg agttccacag
    17401 ttgttttcgg gatcatttca aggcctgtgc tctcaataac ataaccggcg ttggtcaact
    17461 gatccctgac ctccgtcatc gcggcgggat cgcaggttac tgtgaaccct tcaccattgt
    17521 cttccagatc ttcggcaccg gcatcgagac agatgcccat caattgatcc tcgtccagcc
    17581 catttccggt cacccttatg gcgccctttc tctggaagat ccatgaaaca cagccagcct
    17641 ccccgagaga accgccgctc cggctgaaaa gaaaccgtat ctcggaggct gtcctgttct
    17701 tgttatccgt cagagtctcc acgagtacag cgacaccgcc gttcccatat ccttcgtaca
    17761 ttatctcgtc gtaagaggct ccatccatgt ctccggatcc ttttttgaca gccttttcga
    17821 tggtgtctac aggtacgttg gctcctctgg ccctttcaat ggccgccttg aggctcatgt
    17881 tcatagaagg atccggacca ccggtttttg aagccaccat aacagccctt atgtatttct
    17941 ggaaagcctt tcctcttttg gcatcctgtg ccgatttccg gtgctttatg tttgcccatt
    18001 tcgaatgtcc cgacatctat tatcactcct gttctaaatc tgtctgtcaa taaggcatat
    18061 cggcggaatc tctcttttgt gtgaagaccg cctgaatgcg tcatcgatct ttttggcggt
    18121 ggcatcgtca gattctttgg tagcaagata tctgtctatt tgttgatagg tcattccaag
    18181 ttcatcctcg tccgtttgtc ctgcccaaag accggcggaa gggggtttgc ctatcaccgt
    18241 tgcgggcaca ccaagcttac gagccattcc tattacttct cctttgagga gatctcccag
    18301 gggcaaaata tccaccccgg agtctccatg tttggtgaaa tatccggtat gtatctccgc
    18361 gcgattgcca gttccgcaaa cgagaagttt tttttgctga ccaagacaat aaagagttgt
    18421 cattctcagg cgcggtttta tgttggaaag gtggacaccg ttcacggagg atgtctcggc
    18481 aggcatcgcc cttagaagag cgtcgtaggt cgccgaaaga tccacccgta tccacggaag
    18541 atcaaatttc tcgaccacca gcattgcgtc agactcatca atgtcattgc tgtgacatgg
    18601 cataaggacc acagccattt ttttgccaca gaccctggaa agcagggccg caacgaccgc
    18661 ggaatctatt ccgccactca agccgacgat accacctgcc ctcccggact ctttgatctt
    18721 ttggaatacc caggcggaga tcttttcgat aagaaattca ggatccctat agataggcat
    18781 gtcgctcaca ctccggataa atggggtttt atgacgatcc aggtgatatt ctagcatagt
    18841 tgttactggg tttattcctg tttttccgct tcccccatag aggttacggg agtataaaga
    18901 cgtgggtttc tccccgcggt gaaggggaat tttttccgta tcctttcaag gttgctgata
    18961 tctgtgtcta tcgtatttac gcactccccg cttcctggag cctgaaaaac aatgttcccg
    19021 tcaggagccg ccaaaaggga atttccaccg tagatttcat tccctccctt tccgcacctg
    19081 ttacaggcta caataaaaac ctggttctcg atggctcgtg ccctcagaag ggtaatccag
    19141 tggtctattc ttgttgaggg ccattccgcg gggacaaata tcatttgtgc tccggaaagg
    19201 gctatagacc tgacgaattc ggggaaacgg atatcaaagc atattgcaac cgatgccgtt
    19261 acgccgtaaa tctcaaaaag aaaaggacgc tctccgggcc tgaaaaaaag aggttcatca
    19321 agaaggggga aaagatgggc cttgtcatag aaacccgtta cttctccccc atctcctatt
    19381 acgtagcatc tgttgtagat cgggccatca ggttgatctt tccacggaag gctgccggcg
    19441 acaatgtaaa gccctttatt tctggccaca ccctgcagca tgtcgagtgc ccgggggctt
    19501 tgctccgaat gatattgcag tgatgggtta tcatatgaac aggtccaaag ttcaggcatg
    19561 acaacgaccg atggcccatc gggcagaccg ttaaatgttt ccgacatttt tttgatgttg
    19621 gccgcggggt tgccccatgt tatgtctgac tggataatat ggacacgaat ctttttcatt
    19681 tttccacctt tttgaaaaga tttggggggg cgtgttttgg aaaagcttgc aagattcagc
    19741 gtttcactgc cttcggatct acttgaaaga ttcgacagat gggtagaagt aaggggcaac
    19801 aagacaagat ctgacgccct caggcaactg atacggcgtt acatatccga atcgtgctgg
    19861 gaagacgggg accagatcgt gttcgggacc gtcacactct tctacgacca ccattcccat
    19921 gattcagcac aggagataac atctcttcag catgaattcg gggacattat tatttgttcc
    19981 acccacatcc atgtcgacca ccataattgt ttcgaaagtg ttgttttcag gggcaaatca
    20041 tcaagagtaa aaaaatttgc aaaagatctt agcggcctca aggacgtaag cagttcagag
    20101 ccggtaattt cgactatccc ctgataatcc cggacattat ttaaaatcgg caactgccga
    20161 ccgtcacagg gatcaccctg atggagcaca acccttcttc tttctgggtt caccctctat
    20221 caggataagg gttttgcatc tgcattccgg gcaagaaggg ttgatgtccc tctcgcagga
    20281 ctcaaaaacg ttaccgcatg acgcgcatcg gtattttcgt ttttttccca tttgactgac
    20341 ctctcttccc gaaaaaggcg ggccggccga aatgaagctg gccggccttg gattttttag
    20401 atattgcctg cggtagcgac cataactgcc ttgatagtat gcagacggtt ttctgcctcg
    20461 tcgaagactt tcgagctgaa ggactcgaaa acatcctctg tgacttcgtt ccccttgacc
    20521 gcggggaggc agtgaaggaa tatgcattcc gggtttccag tagccttcat aagatccata
    20581 ttcacctgat agggcttcag aagcgctgcc ctttctgcca ttttgtcttc ttcacccatg
    20641 gaagcccaga catcagtata tatcgcgtcg gcattcttta ccgcttccct tggatcctca
    20701 ataaaggcga tcttcgcacc tgtcgttgat gcgattgcca tacactcctc ggaaagttcc
    20761 ttttccggaa aaagccctgc tggtgctgct atcgtaaagt tgactcccat cttggccgag
    20821 ccgatcatga gggaattggc catgttgttt cttccgtcac ccgaatagac cagattcagc
    20881 cctcttagcc tgccgaaatt ttcctggata gtaaggaaat cggcgaggat ctgtgtgggg
    20941 tgatacatgt cagtcagacc attccagaca gggaccccgg aatacctggc gagatcttcc
    21001 actaccgact gcttgaatcc acgaaactgg ataccgtcaa acattctgcc cagaactctc
    21061 gcggtatcct ttacggactc cttgtggcca agctggatgt cgttcttgcc cagatactcg
    21121 gggtgagccc cttcatcgat acatgcgacg gtaaaggcgc accttgttct ggtggatggt
    21181 ttttcaaaga taagggcaac attctttccc gcaagggcgt caccccttac gccggatctt
    21241 ttcttttcct tgagtcccac cgacagatcg agaagatact gaatttcctc ccgggaaaaa
    21301 tccttgagcg tcagaaaact ttgtccctta agatttagcg gcatttactg agacctcctg
    21361 gttgttttgg tgtttggtcg ttattttatg gaaaaaactg ccgggtcccg catcatgggc
    21421 atgctcatgc acctggggcc acctcggcct cttccaagtt cggctccctt gatctcgaac
    21481 acctttattc cgttttcctg caaaaccctg tttgaaacaa cgtttctgcg gtatgtgact
    21541 actactccgg gggcgatagc aagtgtattt gttccgtcgt tccactggtc ccttgctgca
    21601 gccacaggat cgcctccccc ggtctctatg aacctgacct cttcgaggtt cagagccttt
    21661 ttaagacatc ccccaaggct cttgtgttcc tctatggatg tgacctttcc aatttcatcg
    21721 taatccagct cccagaccct gataaagtcc ctgacttccg gatatattgt gaaggcgtca
    21781 cggtctacca tggtaaaaac cgtatcaagg tgcattgccg aacgggtctt gggtatatcg
    21841 acggcaagaa cttttctgac cccggctttt tctgcaagtc ttttcccgat gatctgaatc
    21901 gctccggctg ccgttctctg gctgataccg atagcaacta tcgaatcgga aaggacaagg
    21961 atatctcccc cctcaatgtt ataggggtat gtgtcccggt aatcctctcc aaaaagaatc
    22021 tcgatgtcct tgaagtaagg gtggtgacgg aatatatatt tggcataaag gggttccttc
    22081 cttcttgcct cgaaattcat tacactgatg atcacgccgt tattaacgaa actgaagggg
    22141 tccctctgaa aataaagatt cggaagagga cggataagaa aatccctgtc agacatggcc
    22201 tgcaggacaa ggcttctgca cgggggcatg aactccaggg cttcttcctt ggtaagcccc
    22261 gctatcagta tttctgcaag atcgctgggg gtctcctcct tgagaaccga cttcagggcc
    22321 gaggcaagag gaatgtctat agcttcaagg gcaagagcct catcaagcag agaatcacga
    22381 acttcttcat cccgcaggac atgagaaaga caattcctga aataaaccac ttcaactcca
    22441 ttgtctctta acaggtcagc aaaagaatcg tgctccttct gggcttcctc gacccagagg
    22501 atatcgtcga aaagaaggct gtccttgtta tcaatagtca ggcgctcaag ttccttgccg
    22561 gggcggtgca gcattactct tttaagtttg ccaacttctg aagaaacact gaaggatctt
    22621 tcttcttctg tcatgatata ccccctggat tcttgattat acgtccagtc aagtatagat
    22681 catttgtcaa aaaggaaaaa ggcgggttcc agcccgcctt gtaatttcat taatccaggg
    22741 ccatagcact ttcctttggc agaattgaat ttattgcccc ttcacttccg gtagaaatac
    22801 agcccctttg aatttcgatg gatgtggttc ttgaagattt caggtaggac gacaggtgct
    22861 caaaagcctt ccatggatca gcattgcatc cgcaggtaaa aagatcgatc gccgcatatc
    22921 cgaactctgg ccaggtatgg atcgtcaggt gagattcaga tattacaaga accccgctga
    22981 taccatgggg ctgaaatttc ctgaaggccg actccacaac cgttgctccc gctttttcgg
    23041 cggcagtttc cattgcctcc tgcagttccc cgatgttgtc aagggcctcg cggtcacagt
    23101 cataggcctc gaccagaatg tgtctcccga gggattcccc gtttttcaac aattgtcacc
    23161 tccccacatg ataaggcact cacgagccat ctttgccgcg agcaccgacg ttacattgtt
    23221 tacgtcatgt ggaggcgaaa cctccacaat atcgaaacca acaacattca accccttcat
    23281 ctcgtaaagg gcctcgaaaa tttccctggc aggggtcccg cacggttccg gggttccggt
    23341 accaggtgct tctgcgggct ccactgaatc tatgtctata gtaatataga ccggcttttc
    23401 attgatcctt tcaagacatt ttcctaaagg tctcagcagt tcgcccgggt agaaactggt
    23461 gttggccata ccccactcga attcttccct ggttcctgat cttatacaga actggtaaag
    23521 agaaccgggc gaaaccagac attcttctgc gatccatctc attacggttg catggttgta
    23581 tttcattcca tgatacttct ctcggaggtc cgcatgagcg tccatgtgga tcaccacgag
    23641 gtccccgaat gtctttactg cggccttgac aacaggaagg gtaacaagat gttcccctcc
    23701 aaaagaagcc actcttttgc ccatatcaaa aaaacgggaa gagacgctct cgataataaa
    23761 gagggatcga cacagatcat cacccgggag attcagatcc cctatatcac aaaaatcgat
    23821 ctctgaaaga tctttatcca gaggaaaact gtaagtctcg agaccatggg actcctctct
    23881 tatgacagaa ggggcctggc ccgtgccatg cctcctggtt acggtcatgt cgaggggaag
    23941 accctgaagg atccatttaa ctcccgaatg ggtccttcga ctttcgagga accttttcaa
    24001 ctctcctggt accgccccgc gataatatct tccagaaagg gaggaagaac gaatgctgcc
    24061 ctgtggatct ctttcgaata gtagcgggta tccacgggaa tatcgcttct tattccttcg
    24121 ggatcgtgct ttttggaagc aatggaatag gtccataccc cggtgggata ggtcggcatt
    24181 gctccccaga agacttttac caggggaaag accttcttca tttccgaaac ggcatcggac
    24241 agaatctttg gttctgcgaa cggggactct gtctgggcca ccatgaaacc gtcatcgttg
    24301 agagagttgt aaacatccct ataaaatgac tccttaaaaa ggccctcggc aaaatcgacg
    24361 ggatcggtac tatccacaag gacaacatca aactggcccc gtctttcccg gatgtaatcg
    24421 agagcgtcca tactctttac agttaccttg gggtgatcca gcccaactct gagggatggg
    24481 aaaaactccc tcgaagcatc tataacctcc tggtcgatat caaccaggac tatctcctcg
    24541 acagagcaat gcttcgctgc ctctcttgcg gtcccgccgt ctcctccacc aacaataagg
    24601 acattcttcg gagaagggtg cgatgaaagg gcaacatgtg aaagcatttc atgataggca
    24661 aattcatccc tctcggttac ctgaatagct ccgtcaaggg ccatcaacct gccatattcc
    24721 tcggtatctg ctacgagaag atgctgataa ggggtttttc tattggccag aattccggtt
    24781 atccttaaac caaggcaaag atccggtgtc tggtactcgg aaaaccagag atcagttatc
    24841 cttttgtcca ttatggtatt cccttcagaa ttttgtcggg aaaaagagcg gacagcctgc
    24901 aaatactgta ccgcactcct tcacaacatg gtcaacggcc ttgactttga cctctttcag
    24961 ggccagggat ctcatatcga aagcctccct caccatctcc tcgaccctcg ctgccgcttc
    25021 tgatccgggg caaaaacccg agaactcgaa aatcatccca aaagattcgg gatcctgcgg
    25081 tatacccaca cctatagaag cggatatggt ctcaccggga acatcactgg tgatagaacc
    25141 ataggcaatg ggaagaaggg agccaaaagg cattgacaga gattccctga aggtacagtg
    25201 cggaggcagg acactgctca cccgcaaaag gttcatgttc cccactccgg catcaagaag
    25261 agccgaatca aaggcggtga gttttttctt tccctcgcct ctccctacaa caagcgtaaa
    25321 caactcagga actggcaaca taacaacgca ggcctcccat actttcaact atcagcaaaa
    25381 aagcgcatat cattatatct tgagactgag gggtgtcaat gaaaaataga aatgaaaaaa
    25441 gaaacttcag aggaaaaaag gagaaaacag ggcaggttat tttccgggct taagaaaacc
    25501 ctgacttttg caaacatcca gaaaacggat ctcgaggtcg tcaagggaac accttcccag
    25561 gggcttgcct ttgacgaaaa gcataagtcc cgacggtgtt cccgctattc ctatgtcggc
    25621 tccagaggct tcctgtggtc cgttaacctc acaccccatg accgccacgg tcaattgtgg
    25681 ttcaagcatt tcaagcaagg gctcgatcaa attaacaagt tccggaacgg atgccctcct
    25741 tcttccgcag gcgggacagg aaatcaggtc aggctcgaaa cgccggagcc caagggactg
    25801 aattattctt tttgccaccc tgacttcatc gatggcgggt cccgtcaggc tgatccttat
    25861 ggtatccccg attccgctcc caaggagaga tcccagggca acactgcttt tgataatgcc
    25921 tctttcaccc gggccagcct cggtcaggcc ggcatgaacc ggataatcga aacgttccgc
    25981 aaggagtgtg ttggctttca gcgtctcgtt catggaagat gatttaagag acataataat
    26041 ttccccgact ccgtgaccct caagtgccct gatctggtca tctaccgctg ccagaagggc
    26101 agagaccctg ttgccgccgg ctttggctat ctgggctttc gaaagggatc ctccattcgc
    26161 tcctaccctc acaacggtcc cgttttcaat tatcattttg caaagttcgg caagtcctga
    26221 cttgctggcc atgttaccag ggttgagcct gatcgaccgg catccgacct caattgcctt
    26281 catggccagt ttgtgatcga agtgaatatc agccattatc tccatcggag actcccgaag
    26341 cagaaatgaa agggaatcgg caagcagaac ctccgggaaa gccacccttg ccagttcaca
    26401 gccgttctca tgaagattca gtatttcttc aagacattct ccggtccttc caagaggcgt
    26461 cctgagcatg ctttcaaccc ttacaggaaa tgaaccccct atacccagtg cacctatggt
    26521 tacgcttctg gcactcatct ggaacccccc ggttttttca ggttcaaaag gtcaaaaaag
    26581 acggataagg tctttccatg taacaaaaag gagaagaagt atcaaaagtg caaatccggc
    26641 aaggtgcacc agattttccc atttttcagg aagtttcctt ccggtgacga tctctccccc
    26701 gataatgaga atccttccac cgtcaagggc ggggaacgga agaaggttga gaatgcccag
    26761 gtgaaggttg atcattgaaa gaaatgcaag gaaggtccat aggccctccc ttgcggcctg
    26821 tccggctata gaggcaattc ccactggtcc ggtcacatca acttgtcctc ttcctg
//
LOCUS       DAOTIQ010000019        26791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2075_length_26791_cov_7.499626, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000019 DAOTIQ010000000
VERSION     DAOTIQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 26791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26791
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_26791_cov_7.499626"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="P5541_04115"
     CDS             complement(<1..1268)
                     /locus_tag="P5541_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV71197.1"
                     /translation="MATRNPGDIRTVALVGHGGSGKTALAEAMAFDTGLTTRMGKVED
                     GNTVSDFGTEEQKRQISINSSLLSLEHSGKHFYIFDCPGYADFIGELRSAMRVSDTGI
                     VVVSGVHGVEVQTEKAWEFGEDFQTPMAFYISKMERENADFEKVVGEVQQYLSEKATP
                     LFLPVGKEATFRGVVDILRQKAYLYKGDGSREFTEADVPDDMAEQVSSAREALVERIV
                     EAEDELMMRYLDGEALSMEELVPALRKAVLNRSLFPVMPGASTANVGVVQLLDAIAEV
                     FPAPVEMPPRMAMKGDAEHLVVPDPAGKFSGFCFKVMVDPYVGKLSFIRVFAGTASSD
                     NPIHNVTKGEDERVSSFKLMRGKEAVDSKDVVVGDIVAIPKLHSTVVGDTLGVKGNDF
                     TYPEIAFPKPVYSVAVKPRSRADEDKLANAL"
     gene            complement(1391..2887)
                     /gene="gatB"
                     /locus_tag="P5541_04120"
     CDS             complement(1391..2887)
                     /gene="gatB"
                     /locus_tag="P5541_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRV71198.1"
                     /translation="MGLEFETVIGLEVHVQLATRSKLFCPCSTDYIGAEPNSNVCPLC
                     LGLPGTLPSLNRKAIDLGLRAALALKCSINRKTRFHRKNYFYPDLPKAFQISQYDVPL
                     AVSGSMPITIDGRVKNIGITRLHLEEDAGKLVHSTSDGRLSGAMFSLVDYNRSGVPLA
                     EIVSEPELSSSEEAREYVISLRRLVRYLGVSDGDMESGSLRVDANVSQKCSDGRWGNR
                     VEIKNLNSLKALERALEFEISRQRDLLGKGVEIIQETRHWDDGAGVTRSSRGKEEAHD
                     YRYFPEPDLPAIRIQSAEVDRMAGALPELPWEKRERYAKEWGLNLPDASSLSDNPDVA
                     EFFEKTVASGAEPLLAANWIRTEILRLVNENEVKVLDLSVKPEHLSALLNKVRGGDLS
                     NTAAKDVLGVMAKEGIGLSDAMKKAGVREGGFSTDALEEVVRAILEANPSEADLVLGK
                     KDPGDKKRKFLTGLVMREIRGQASPDAVAAAFEGILESREDMGNAHSH"
     gene            complement(2887..4356)
                     /gene="gatA"
                     /locus_tag="P5541_04125"
     CDS             complement(2887..4356)
                     /gene="gatA"
                     /locus_tag="P5541_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRV71199.1"
                     /translation="MAFNEMDAARIASGIREAEFSAREVIQSSISRIRSLEPTLNAII
                     TPLFEEALSRADRVDRIISEGGNPGPLGGVPVVVKDNMCMKGIPTTCGSRILEGWKPP
                     YDASVVSSLEEAGAVIIGKSNMDEFAMGSSTEHSAYGPTSNPRDTGRVPGGSSGGSAA
                     SVAAGYVPIALGSDTGGSIRQPAAFCGVHGLKPTYGLVSRWGLVAFASSLDQIGPFAR
                     SISDMALVLDVISNNDPRDSTCSSSERPSFSDCIKDPSVRGKKVGIVREFIGGSLDPS
                     LKGAIEKTASMLEASGAEIVDVALPMSGEYGLPCYYIIAPAEASSNLSRFDGVRYGMR
                     AEASDLITQYIKTRGSGFGEEVQRRILTGTFALSSGYYDAYYLLALKVRRLICSEFAR
                     AFERADVILMPASPTLPFRKGELVDDPISMYMSDVFTLPVNLAGLPGITVFTGFEGSG
                     LPLGVQLVAPRFQDSRLLEFAGVIESWAGRPGISPGGDK"
     gene            complement(4359..4655)
                     /gene="gatC"
                     /locus_tag="P5541_04130"
     CDS             complement(4359..4655)
                     /gene="gatC"
                     /locus_tag="P5541_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRV71200.1"
                     /translation="MAITEKDVLHVAELARLEIGTERIEPMVRYFQDIIGHFKCLEKC
                     DTDGIDPFTDDDMHPCPLREDKTARWGFSGEALESASLSEGRFFKVPAIGGEGE"
     gene            complement(4642..6705)
                     /gene="ligA"
                     /locus_tag="P5541_04135"
     CDS             complement(4642..6705)
                     /gene="ligA"
                     /locus_tag="P5541_04135"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRV71201.1"
                     /translation="MAEEETRKRIDWLREEIARHDHLYHVLDAPEIEDSKYDLLVREL
                     KELELREGGETEPPSSVSRVGGEPLKSFRRVVHDVPMLSLDNAFTHEDLDSFLRRTGG
                     APMCCELKIDGLAVSLVFRDGFFSMGATRGNGRVGEDVTENIRTVSGLPLELTEKIPG
                     RLEVRGEILIRSEDFATLNAEREEQGLPLFANPRNAAAGSLRQLDPRVAASRRLSLFV
                     YQVVSPGDLGLNSQYETLGRLRDLGFPVQGVESLCETPEEVMGFIEKWRTRRFSLPYV
                     TDGVVIKIDDLSLRKTLGTTAKAPRWAIAYKYPPEEKKTRLLKIDVSVGRTGTLTPVA
                     ILEPVTLSGTVVQRASLHNADEIARKDIREGDMVWVRKAGEIIPEILRADPGSRHEDS
                     RPYVMPGNCPSCGSVPVSLPGEVALRCINRACPAQILEGLRYFASRSGMDISGLGEKV
                     IEKLVADGRVKDFSDIYSLEMKDLAHLRLSGERSSRVLGEKAAASVLSSIEASRKKPP
                     ESLLAALGIRYVGSRVAEILVGAFGGLYELERASEEDLSAVEGIGPVIAASVVAFFSD
                     SRNRDILERLRSSGVATGTSQINPGSTVAPLKGISFVFTGEMDVMTRSEAEAAVKRLG
                     GLAPSSVSRKTSILVRGKNPGSKLAKAQSLGVRIIDETAFGEMIESAEGSSKEKKGDG
                     ASGDN"
     gene            complement(6730..8238)
                     /gene="lysS"
                     /locus_tag="P5541_04140"
     CDS             complement(6730..8238)
                     /gene="lysS"
                     /locus_tag="P5541_04140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRV71202.1"
                     /translation="MTDERTNDENVVSEDEILKQRTDKLRRLREEEGYDPFAHEQWIK
                     KHTLEFVRENFSHLEPDDRDDTCIVTAGRLMTLRKHGKASFANLADETSGMQLYFQVD
                     AMGEGPYEFFKKWVDTGDMLGIEGHPFRTRRGELSVLVTRCVLLAKALRPLPEKWHGL
                     KDTETRYRQRYVDLIANPDVRDTFRKRARIISSFRQVLESHGSIEVETPTLATLAGGA
                     NARPFVTYHNALGVDMFLRIAPELYLKRLIVGMLGRVYEIGKNFRNEGIDIMHNPEFT
                     SMEVYWAYANYEDIMNLTEELVVAAADAVGSRDIVYQGTPISLNPPFRRATMAELVLE
                     NTGFDIHRTDDASARDFAKKKGLEVEGHESRFIVLTELFESFVEEKLVQPTFVIGLPT
                     EVSPLAKKNQENPDFTNRFELYIYGSEVANAFSELNDPLDQRERFMDQMKKKEAGDDE
                     AHAFDEDYINALEYGLPPTGGLGIGMDRLTMFLTDSRSIRDVILFPTMRPRS"
     gene            complement(8228..9247)
                     /locus_tag="P5541_04145"
     CDS             complement(8228..9247)
                     /locus_tag="P5541_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRV71203.1"
                     /translation="MTGIVGQDEINIGGVSVDNPVWLAPLAGITVPSVRLFFRRLGAG
                     LTHTEMISCAGLIHGSIKTFRMLRILDGERPVVLQLFAGDEKTLLEGAGLALSASPSF
                     DAIGINMACPMPKVLRKGAGARLLERPDIAGSMVRSLRGNGLPVWVKTRKMPSGSPMD
                     TRDFTGMLLDAGASQVCIHGRSPVQRYGGSSDREVIIETASAFPGMVAGSGDVFCSRD
                     AAELLEGGCAAVFVARGAFRDPFLIPVILDELGFDVDRKLLSREPGSRIEMMQELGAL
                     IVEEEGERAALLLLKRFLSGIFKGLRGASLFRRSVGMAANWREMHDILQEWKVFAERG
                     EHLDR"
     gene            complement(9216..9992)
                     /locus_tag="P5541_04150"
     CDS             complement(9216..9992)
                     /locus_tag="P5541_04150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRV71204.1"
                     /translation="MLLVIDIGNTNTVLGIFDGEKMLRNWRLVSERHTADELGIYLLN
                     LISLAGITPEDIDGAALSSVVPPLDAPWEEGIGRYLSLDCLKVTSETDLGIPVLYSTP
                     SEVGADRLVNAVAGIKRYGSPLIVVDFGTAITLDVISPSGEYLGGTISPGLALSMEAL
                     FGKAAKLPKVSFDLPEKVIGKTTRESIQSGMIYGFSGMVDSLVERVNLEQDVSATVIA
                     TGGHAEVIAPLSRTISAVEPWLTLEGLRIIYDRNCGAGRN"
     gene            complement(10026..11438)
                     /locus_tag="P5541_04155"
     CDS             complement(10026..11438)
                     /locus_tag="P5541_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRV71205.1"
                     /translation="MNPAAVAPALVVFAGCSLSLWLRAAGASLFVALAGGALVSAFIC
                     LAGSAHYCPQGFRDVFIFLVAVTAIGVVLMVSRLEPDRFEPIRISTSGTVVRERSWGR
                     GRVILLDSPVGKIVVHLLPSSLFREGEILEVEGVLEPFSPEPGSGGFDEGTYWLARGA
                     VGIIRHGSGRSSGEMSVGISRWRELVRERILESMPPMMRGHLLAAWLGDRDPDLALMH
                     SAWGTSHVLAVSGLHVGVVVLLLFTLLPVRFFRLATSSLFMWLYILFAGASSSSLRAG
                     LMLQTGFFARWKGRPLQALNMVSVAGLLLLFWRPWFFWDLGWRLSMTAALVLSSLQGT
                     RPGKGWILAPLILWVSTAGLVSGTFGSVPVAGIFVNIIAVPLFGILLPGASLLALPAL
                     AGVPGGSFFAWTGEILFRAWAILADGVSFLLPWNVPCSAGFSAAGMAVALSFVLKGTG
                     FGWFYAVLFSGAIAIFFMVM"
     gene            complement(11435..12073)
                     /locus_tag="P5541_04160"
     CDS             complement(11435..12073)
                     /locus_tag="P5541_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV71206.1"
                     /translation="MPNTTGRRKALLFIVAGLVCLVAAGAIVASFSGRWERTRSGVVA
                     GPAPGTANDATSREIPVEGSLEETQWVIYITGAVFRPGVYRLPEGSRVYQAVDQAGGL
                     TADADPESFNMALPLHDGIHIHVPRKGELQSLGSTDNGVRQEGGSRLIDINSAGAVEL
                     QSLPGIGPKTAAEIIRCREEQGRFKNVEDLLSVKGIGAAKLSGIKDLVTVSR"
     gene            complement(12073..13398)
                     /gene="miaB"
                     /locus_tag="P5541_04165"
     CDS             complement(12073..13398)
                     /gene="miaB"
                     /locus_tag="P5541_04165"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRV71207.1"
                     /translation="MRSFAIKVYGCQMNVYDADRIRTALVSTGWEEVLEDVASLVIFV
                     TCSIREKAEQKVLSEIGRIGSRSAEGQPVIAVVGCMAQRIGDSLLDRFPAVRLVCGPR
                     NLGRLLPGIDRIMDSRERLSILDENPVSLEDLGVSPLYRENPWKAYITIAHGCDNFCT
                     YCIVPHVRGRFMSRDPEGIFSEVQELVSSGVREIILIGQNVNTYGHDFGNDYRFSSLL
                     DDVASMKGVNRIRFCTSHPKDLTEDVVRVMSRRPSICTGINLPIQSGSDRILGLMNRG
                     YTVEKYASIIEMIRDHLPEPSVTSDLIVGFPGETNDDFRESVRVVEKFRFDLVHTAAY
                     SPRDGTPAARMDGQVPFAEKKRRLAEINRIQEDITMAINDALVGREFEVLVDGFAPRG
                     DSMLQGRTSADKVVIFPGDASLLGSFRMVRITGSGNWSLRGEIIPEGTQ"
     gene            13543..14052
                     /locus_tag="P5541_04170"
     CDS             13543..14052
                     /locus_tag="P5541_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71208.1"
                     /translation="MAPGHFLARLFNVVTLTMTFAALLAVSSPATDNLALPEIEGFRS
                     GNATATILDTPSGNQGTWIQQSYFRTPDNHSVDLNILMGPGTGWHGLPEEGISSTDGP
                     IGSGAVYMTLRVSGFPAALEIHPLTGKTLTVSPSGQETLTFETTFEDIDLVEFAETFL
                     GLCQVGSNP"
     gene            complement(14056..17223)
                     /gene="carB"
                     /locus_tag="P5541_04175"
     CDS             complement(14056..17223)
                     /gene="carB"
                     /locus_tag="P5541_04175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRV71209.1"
                     /translation="MKTGRKPILVLGSGPIRIGQAAEFDYSGSQACRALRDQGYHSVL
                     LNSNPATIQTDTAMADVVYIRPLETDVVEEIIRTHRPGGVIATLGGQVALNLCVELQK
                     KGIWERYGVKVLGTSPESVQRSEGREDFRQAMVGIGEPVIESAYVDNIDDAFAFAEKT
                     SFPLIIRPDYTLGGTGGGVAFDAASLRERVGEGLRLSPVSRVLVERYLQGWREIELEV
                     VRDCAGNRLCVCGMENVDPMGIHTGDSIVVAPVLTLSDRQWQRLRSSAFRIVDELDIR
                     GACNVQFTISPDAGEYAVIEVNPRASRSSALASKATGYPIARIAARIALGDLLTEMPN
                     PVTKSGSALSEPAVDYVVAKFPRWPFEKFPGAEYTVGTRMKSTGEVMAIGRTFIEATL
                     KAVRSLDLPGGLFDPGVKGMSDDDLLEELSVPTHRRLWAILELLGRGVPIGVVSDRTG
                     IHPFFVDQFSRIPLAFDELKNRGPEKNLLRRLKEMGISDADIASCLGIPEGDLRSRRI
                     ASGIVRGYREVDGCAGETPAESGYYYGSCGVLDDGVRDYPGEAIAVIGSGPIRIGQGV
                     EFDYCCVKAVEAIRRCGKRAVMINNNPETVSTDHDMSDSLYFEPLAVEDILPILEREK
                     VTGVFAAFGGQTSLKTGLALEPEGVKILGSPSSVVRASEERGEFSRLLDSLGILQPQG
                     FAVSDVGEGLEKASMIGYPVMVRPSFVIGGLAMRVAAGPSELGSVLDLALKSGEGQSV
                     LIDRFLPGSEFEVDVVCDGSEVVIPGIFEHLDPAGVHSGDSVAIFPDVSLSPSRRKMI
                     EEISKKIALGLGVRGLLNIQFVLSGDEIYVIEANPRASRTVPIAGKLTGMPLVEMAVR
                     ASLGETFGEMGYSSGIQERKGPFGVKVPVFSTEKLPGVDALLGPAMQSTGEALGVGST
                     VAEALYEGLRGAGWKVPVKGRLLLSVADRAKTESAHVAAAFYALGWKIYATAGTAALL
                     GNWGIPSEMVDKGHSLVSGIASRQWDLIVNIASGNLEVLNDGYRMRRAAIEASVPCIN
                     NLSLASALAISVAIEKGKRQDPGLA"
     gene            complement(17220..18263)
                     /locus_tag="P5541_04180"
     CDS             complement(17220..18263)
                     /locus_tag="P5541_04180"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRV71210.1"
                     /translation="MKEMKIVLEDGSFWSGTGHGGFASGEVVFTTASSGYPQAISDPS
                     FAGQIIVFAFPMVGNYGVDECFLESGRPWARAVLAGAVEAGDYLGPRLEEWLAGYDIP
                     LVTGVDTRSLVRHIRTKGALQGTVAPSGETPVFGMTSTHPVEEVSIKAPELMDGPGKT
                     IAVIDYGLKQGIQRELLKRGCRVVRLPHNSSKEDILSWHPDGILLGNGPGDPSALVKE
                     TEVVSLLLKEVPIGGICLGLQIIALACGASTYKLPFGHRGENHAVIDLRSGRGLVTSQ
                     NHGYAVSESSLKGTGLEVTHRNLSDGSVEGVRSPGGEVLAIQYHPEGSPGPADGEVFF
                     EEFLSMCERSSAR"
     gene            complement(18440..18601)
                     /locus_tag="P5541_04185"
     CDS             complement(18440..18601)
                     /locus_tag="P5541_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRV71211.1"
                     /translation="MKKYLCTVCGYEYDPAVGDPDGGVAAGVSFDDLPDGWVCPVCGV
                     GKDMFEVVD"
     gene            complement(18689..19816)
                     /locus_tag="P5541_04190"
     CDS             complement(18689..19816)
                     /locus_tag="P5541_04190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRV71212.1"
                     /translation="MEMNRAMQTERRIEAMRSSMKSAGVDAVIVPVREGLNWETCFYL
                     TGFRGSSSVFYATSDQAGLITDGRYLAQAASQTRIPVIDQASTPMMDVLGDIISGIGG
                     IGTIGFQADRVTCDLMGKIRGFGKSTVDVSPLFSRLRRKKDGGEIGDIRVAADLASSA
                     LLKTLSEGEKDLTESSFAARLEYNIRMAGAEGGWGSHEFIVASGPRSALPHGTPTGRK
                     IEPGEMITIDFGSRFNGYISDITRSFCDGEPPEWAVEAHELLTEAQSAAIALLEPGRP
                     ARDVDMAARSVIEKAGFGDAFSHSLGHGIGLEVHEAPFLSFRSEDILAEGDVVTVEPG
                     IYFPGKGGMRLEDDFYITAEGRECLTSSLLSTILPLGQRTE"
     gene            complement(19807..20799)
                     /locus_tag="P5541_04195"
     CDS             complement(19807..20799)
                     /locus_tag="P5541_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRV71213.1"
                     /translation="MKGEQNTAVSGKLAPEILEKAVLAYGGARRKEVLIGPGVGEDAA
                     VIDWPVGKYLVVASDPIVGASSGAGRLLVTVNSNDIASKGADPAYLISTLIFPAGTTP
                     ESVSSVVQEIHEACLEAGISVVGGHTEINDKYAHPVLCATMIGMAERVISARDVRPGD
                     VVLMTKHVGIEGMCILAGDCRQYLGKCLDEAEIDEILGWGSLLDVLPESRILRKWASF
                     MHDPTEGGFLGGLGEICRLSGFGIDLDTSSIEIHPLTRHCSTCLGFDPLRLISSGVLV
                     AVIPREHLEEARVSLEKAGIKSAVAGSITEGPGSVGTETVEELWRLLAEGCRWK"
     gene            complement(20807..21358)
                     /locus_tag="P5541_04200"
     CDS             complement(20807..21358)
                     /locus_tag="P5541_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRV71214.1"
                     /translation="MTLKVVLHVCCAPDATIPWPALMEEGHKVTGYFYSSNIHPFEEF
                     RRRSEDVERLALLLGAEFLVDEYRPLNWLEKVSSHILSPEGGERCALCFREQLEGAAG
                     MAVERGFDALCTTLTISPHKDVGLVDVIGREVCDERGLEWLSRIWRKNGGFPASVRKS
                     RQLGLYRQNYCGCVMSIRDEGTE"
     gene            complement(21355..22170)
                     /locus_tag="P5541_04205"
     CDS             complement(21355..22170)
                     /locus_tag="P5541_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HRV71215.1"
                     /translation="MDNLIAPGDLVFIWAPPKGDSFIVRIVPGGRQDSRLGQILHDNM
                     IGKSYGGWISTNLGKKFCMLRPNVQEFSRRVRRQTQILYPKDIGFILVSLNIFPGALV
                     VECGTGSGSLTSVLAHFVGDEGKVVSYERREEFSLLAKRNCEKWEIAHRVEFKVRDIA
                     DGFDERNADALFLDLPNPWDYLEQTKATLAPGNRLGILVPTTNQLEKVIDGLETNSFA
                     DIQIVEIMLRHYKVNSRRIRPEDRMIGHTGYLVFASSVEALPEVEGEGAGDLI"
     gene            complement(22199..24310)
                     /locus_tag="P5541_04210"
     CDS             complement(22199..24310)
                     /locus_tag="P5541_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV71216.1"
                     /translation="MKAKNLFIIMIAALFALIFVHLGFSVSLGIRSEIKNLDSALVLA
                     TRTISFVVGNDLQEIDRIVSTDTGVSASRDLLPKGAGPLALVPNETRLSRPLPMHGNW
                     HVLSVEQGQGELAFARRAPEGYPAAFIKYRPDLSWADFLLPGMDGAELRIVAADGSLL
                     WRTGQPLSLPGKAGSGISTPSDAIWSPVYKRRADIGPGGLSVEVVFPAVPFVLSALAR
                     LPWTIGVELLLIMGLFAAWIIFSRFLLDPLERVTRLSLQMQDRLFTSTSPADLAEGVA
                     NLAGGIRDLTRNSRLSEVKVFCQTLSSALQGYVYQQEKIMDSGKELERANRALSEANR
                     GLLRRDEIWRKILDVSRSVTIESGFQKGLERVASILADVAGAYGVAICRIEGDRIVVL
                     AWSGFDGLLEIKDTLLDGSIIGRALDSGSPVWVENVYSDRSYCQLNRKIKSELNIPMF
                     HIGKAVGGLAISWNQVKTEDKALVELVMPVALHVAGLLNTQASMMDLKNSYKYMAGRL
                     QNLTAIYHDETAAHLERMEHYCRFLSVAMSRSMIEVEDVALFSRLHDIGKLKVPMEIL
                     SKPAGLNKAEFDIVKHHTEWGAEILGEASWLQMGRNICRYHHEKWDGSGYPEGLSGES
                     IPWEARIVALADTYDALRSSRSYKPPYTHLETVRIILQGDQRLRPEHFDPALLDIFRK
                     FHHRMAEIYETFRDEIRNTPA"
     gene            complement(24444..26300)
                     /locus_tag="P5541_04215"
     CDS             complement(24444..26300)
                     /locus_tag="P5541_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRV71217.1"
                     /translation="MRKVFGVGFTVFAAIAFVLAGYHGVSAEVTTVAPEVVTGSRIYT
                     DLNEVPAPAYVITAEDIAASGASDLGALLDSRIPGIFMKKKSGVAQQSQIWFRGFTNQ
                     VLVLSNGIPLYSSSIGVDFGAVDFRSFALEDIERIEVVKGGASAIYGSMAAGGVINII
                     TKKPEKPGGKVVAEAGPSGWRRYYVSGNTGGDVLNAGIWYERVEEGRKRLFYDTSPTN
                     RYDSLGYKGDAYGLNLSGKHWIFKASWGDYKYQYEGNWTAFNDEKKTYSRYSFRYDWD
                     NWYLLTGYDTQRYDILQNPNNYFEDSAYTAEVGGKSALGEALVAWGLYYRREDTEFKD
                     DGAVNPVVSKDRYNLAPFIEASYPLGEWVANLGLRYEIWRQDSNDHDELIPKLSLQRQ
                     FANGNMIYISASRVFAMPTFYMLFADYDMGWGDKTVGNPDLKPEKGWSYELGLKGIDL
                     LEPWQIGVFLTVLDEKIKYDYRFPFPSTYINVAEFRTYGIEATKKWKLGTNWTLGLNG
                     TWQMPEEKATATSDWVRSTAVPEWEIGGSLEYKSGPWTALLIHSWSGNQGNRDSDHLS
                     QVDLALSWESEVDTVRLNLINVFGKENIFEAGFGSYYYGPERGIKLSWERRF"
     regulatory      complement(26395..26559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         6265 a         7951 c         6475 g         6100 t
ORIGIN      
        1 agggcattgg caagtttgtc ttcgtccgcc cggcttctgg gtttgaccgc aacgctgtag
       61 acaggtttgg ggaaagcgat ttccgggtaa gtaaaatcgt tcccctttac tccgagagta
      121 tcccctacga cggtgctgtg cagtttcgga atggcgacga tatcaccaac gacaacatcc
      181 ttggagtcta cagcctcttt acccctcatc agtttgaacg aactgaccct ttcgtcctct
      241 ccttttgtga cattatgtat cgggttatcg ctggaagcag ttccggcaaa gaccctgatg
      301 aaggagagtt ttcccacata gggatctacc attaccttga aacagaagcc ggagaacttc
      361 cctgccgggt ctggcaccac aaggtgttcg gcatcacctt tcatggccat cctgggaggc
      421 atttctacgg gcgcggggaa aacctcggcg atagcatcaa gcaattggac aacgccgacg
      481 tttgccgtgc ttgcaccggg catcaccggg aaaaggcttc tgttcaggac ggccttacgt
      541 agagccggaa caagttcctc catggaaagg gcctctccat cgagataccg catcataagt
      601 tcgtcctcgg cctcaactat cctttccacg agtgcctccc tggcggacga tacctgttcg
      661 gccatatcat ccgggacatc agcctcggtg aattccctgc ttccgtctcc cttgtaaagg
      721 taagccttct gcctcaggat atccaccacg cccctgaagg tcgcttcctt ccccaccgga
      781 agaaaaaggg gcgttgcctt ttctgaaaga tactgctgca cctctcccac tactttttcg
      841 aaatcggcat tctcccgttc catcttgctt atatagaaag ccatgggagt ctggaaatct
      901 tcgccaaatt cccaggcctt ttctgtctga acctcaacac cgtgcactcc actgaccacg
      961 actatacccg tgtcggagac acgcattgcc gacctgagtt ctccaatgaa atcagcgtaa
     1021 cccgggcagt caaatatata aaaatgtttt cccgagtgtt cgagagacag aagcgaggag
     1081 ttgatggata tctgtctttt ctgctcttcg gtaccaaagt cacttaccgt gttaccatcc
     1141 tcgacctttc ccatcctcgt ggtgaggccc gtatcaaagg ccattgcctc ggccagcgcc
     1201 gtctttcccg aaccgccgtg gccgacaagc gccacagttc ttatatcacc tgggttgcga
     1261 gtcgccatac acttttacct ccctcaaatg atgaaaagtg tttattttca ggttggttcc
     1321 accctgtccc tgtaataccc gaattttccc tgatctacct ggaatatctt aaggggcaag
     1381 gggcggagcg tcaatgagag tgcgcattcc ccatatcttc ccggctttca agtatacctt
     1441 caaaggctgc cgcaacagca tcgggggatg cctggcccct gatctccctc atgaccagcc
     1501 ctgtaaggaa tttccgtttt ttgtcccccg ggtccttttt tcccagaaca aggtctgcct
     1561 cggagggatt tgcttcgagg atcgccctta cgacttcctc cagagcgtcc gtggagaaac
     1621 cgccctccct gactccggcc tttttcattg catcagaaag tccaatgcct tcttttgcca
     1681 tgactcccag aacgtccttc gccgcggtgt tggaaagatc cccacccctc accttattca
     1741 ggagagcaga caaatgttca ggttttaccg aaaggtccag gaccttcacc tcattttcat
     1801 tgacaaggcg aaggatctct gtccttatcc agttcgcggc aagcaggggt tcggcccctg
     1861 aagcgaccgt tttttcaaag aattccgcca catccgggtt atctgaaagg gaagaggcat
     1921 cgggtaaatt cagaccccac tctttagcat atctttctct tttctcccag ggaagttccg
     1981 gcagggcacc ggccattctg tcgacttcag cgctctggat ccttatagcc gggaggtcgg
     2041 gttcaggaaa atacctgtaa tcgtgagctt cttcctttcc cctggaagaa cgggtaacac
     2101 ctgccccatc gtcccagtgg cgtgtttcct ggattatttc tacacctttg cccaggagat
     2161 ctctctgtct cgatatctcg aactccagtg ctctttcaag ggctttcaga gagttaaggt
     2221 tctttatctc cacgcgattg ccccacctgc cgtcggagca tttctgggaa acgtttgcat
     2281 cgacccgcag tgagcccgat tccatatctc cgtcagagac accgagatac ctgacaagtc
     2341 tcctcaggga gatcacatat tcccgggcct cttcagaaga agacagttca ggttccgaaa
     2401 ctatctccgc aaggggcact cccgacctgt tatagtccac cagtgagaac atggctcccg
     2461 aaagcctgcc atcagaagtg gaatgtacca gcttccccgc gtcctcctcc aggtgaagcc
     2521 ttgtgatgcc gatgttcttg actcttccgt caatggttat cggcatggaa cccgatacag
     2581 ccagaggcac atcatactgg cttatctgga aggctttcgg aaggtcggga taaaaatagt
     2641 tcttcctgtg gaacctggtc ttgcggttta ttgagcactt cagggccaac gctgccctta
     2701 gtccaagatc tatggctttt ctgttcagcg acggaagtgt ccccgggagc cccaggcaga
     2761 gagggcagac attgctgttg ggttcggcgc cgatatagtc tgtcgaacag gggcagaaaa
     2821 gtttgcttct ggtcgccagt tgcacatgca cctcaagacc tattactgtc tcaaattcca
     2881 gtcccattac ttatcacccc ccggtgatat tccgggccgt cctgcccagg attctattac
     2941 accggcaaac tccagcagtc tggagtcctg gaatcttggc gcgaccagtt gcactcccag
     3001 gggcaggcca gatccttcga aacccgtgaa aaccgtgatc cctggaaggc cggccaggtt
     3061 gacaggcagg gtaaaaacat cgctcatgta catacttatg gggtcgtcga caagttctcc
     3121 tttcctgaag ggaagggttg gggatgcggg cataagtatc acatccgccc tttcaaaggc
     3181 cctggcgaac tcggaacaga tgagtctcct gaccttgaga gcgagaagat aataggcatc
     3241 gtaataacca gaactcaacg caaaggtccc ggtgagaatc ctcctctgga cctcctcgcc
     3301 aaaacctgaa cctctggttt ttatatactg ggtgatgaga tcggaagctt cggccctcat
     3361 accatacctg accccgtcga aacgggaaag gttcgagctg gcctccgccg gcgcaataat
     3421 atagtagcat ggaaggccat actcgccaga catcgggaga gccacatcga ctatctcagc
     3481 ccccgatgct tcgagcatgg atgcagtttt ttcgatggca cccttcagag aaggatcaag
     3541 ggaacctcca atgaactctc ttactatccc gaccttttta cctcttacag agggatcttt
     3601 tatacaatcg gaaaaagagg gcctttccga tgaactgcag gtggagtctc tgggatcgtt
     3661 atttgatatg acatcaagga caagggccat atcagatata ctcctcgcaa aggggcctat
     3721 ctgatccagg gatgaggcaa aggccacgag accccagcgg cttacaaggc cataggtggg
     3781 tttgaggccg tgcaccccgc agaaagctgc cggctggcga atggatccgc ccgtgtcgct
     3841 tccaagggct atcggaacat accctgcagc cacagatgca gcgctaccgc cggaactgcc
     3901 tccgggcacc ctgccggtat ccctcgggtt cgaggtggga ccataagccg aatgctcggt
     3961 agaactcccc atggcgaact cgtccatatt ggattttccg ataattaccg ccccggcctc
     4021 ctcgagagaa gatacgacag aagcatcgta aggaggtttc cagccttcga ggatcctgct
     4081 cccgcaggtt gtcgggatcc ccttcatgca catgttgtcc ttcacgacaa cagggacacc
     4141 ccccagggga cccggattcc ctccttctga tatgatcctg tcaaccctgt cggcacggga
     4201 aagggcctct tcaaaaagag gggtaattat ggcgttaagc gtaggttcaa gggatcgtat
     4261 ccttgatatt gaactctgta tcacttccct ggctgagaat tcggcctcgc ggataccgga
     4321 ggcgatcctt gcggcatcca tttcgttaaa ggccattatt attctccctc tccgccaatg
     4381 gcaggaacct tgaaaaaacg gccttcactg aggctggcag actcaagggc ctcaccggaa
     4441 aaaccccatc gcgcggtttt gtcttccctt agcggacaag ggtgcatatc atcatcggta
     4501 aatggatcta ttccatcggt gtcgcacttc tcaaggcatt tgaagtgccc gataatgtcc
     4561 tggaaataac ggaccatagg ttctatcctt tcggtcccga tctcgagcct cgcgagctcg
     4621 gcgacatgga ggacgtcttt ttcagttatc gccacttgct ccatctccct tcttttcttt
     4681 gctgcttcct tctgcactct cgatcatctc gccgaaggcg gtctcgtcta tgatcctgac
     4741 gcccagggac tgggctttgg caagtttgct tccggggttt ttcccgcgaa caagaatgga
     4801 tgtcttcctg ctcaccgaag agggcgccag ccccccgagc cttttgactg cggcttcggc
     4861 ctctgaacgt gtcatgacat ccatctcgcc tgtaaagaca aaactgatcc cctttagagg
     4921 agcaacagtg ctgcccggat ttatttgtga agtgcccgta gctacacctg aagaacgaag
     4981 cctttcaaga atatccctgt tgcgggaatc gctgaaaaaa gccacaacgg atgcggcgat
     5041 caccggaccg atcccctcaa ccgcggagag atcctcttca ctggctcttt cgagttcgta
     5101 taatcctccg aaagccccca caaggatctc ggccaccctg gatccgacgt aacgtatgcc
     5161 gagagcggca agaagagatt cgggaggttt ttttctggag gcttcgatag acgaaagaac
     5221 cgaagccgcc gctttttccc cgagcaccct tgatgatctc tccccggaca ggcgcaaatg
     5281 tgccaggtct ttcatttcaa gagaatagat atccgaaaaa tcttttaccc tgccgtctgc
     5341 aaccagtttc tctatcactt tctccccaag tccggatata tccattcccg aacgggacgc
     5401 aaagtacctg agaccctcaa gtatctgggc gggacaggcc ctgttgatgc acctcagggc
     5461 tacctccccg ggaagggaaa cgggcacaga cccgcaggag gggcaattcc ccggcataac
     5521 atagggtctg ctgtcttcat gcctggatcc gggatcggca cggaggatct ccggtatgat
     5581 ctcaccggct ttccttaccc agaccatgtc tccttccctg atgtccttcc ttgctatctc
     5641 gtcggcattg tggagacttg ccctctgtac aaccgtgccg gaaagtgtaa caggttccag
     5701 tatggctaca ggagtcagag tcccggtcct gcccacggaa acgtctatct tcagaagccg
     5761 ggttttcttt tcctccggag gatatttgta ggctatagcc caccgggggg ccttggctgt
     5821 ggttcccagg gttttcctca gggagagatc atcgatcttg attacgaccc cgtcggtaac
     5881 gtatggaaga gagaagcgtc gggtcctcca tttttcgata aaacccatca cctcttccgg
     5941 agtctcgcaa agggattcga caccctggac cggaaacccc aggtcccgaa gacgccccag
     6001 agtctcgtat tggctattca gtccaaggtc gcccggagaa accacctgat aaacgaaaag
     6061 gctcaatctc cttgaagctg ccaccctcgg atccagctgc ctcagactcc ccgcagccgc
     6121 gttcctggga ttcgcaaaaa gaggtagacc ctgttcctcc ctctctgcat tgagagttgc
     6181 aaaatcctcg cttctgataa ggatctcccc cctgacctca agccttccgg gtatcttttc
     6241 ggtcagttca aggggaagcc cggatacggt ccttatgttc tctgtaacgt cctcgccgac
     6301 ccttccgttg ccccttgtcg cacccatgga aaaaaacccg tcccgaaaga caagcgacac
     6361 ggcaaggcca tcgatcttca gttcgcagca cattggagcg ccaccagtcc tgcggagaaa
     6421 ggagtcaaga tcctcgtgag tgaacgcgtt atccagactg agcatgggaa catcatggac
     6481 tacccttctg aaactcttga ggggttctcc accaacacgg gagaccgaag acggtggttc
     6541 cgtctccccg ccttcccgaa gctcaagttc cttcagctcc cgaacaagaa gatcgtactt
     6601 cgagtcctcg atctccggtg catcaagaac gtgatagaga tgatcatggc gcgctatctc
     6661 ttccctgagc cagtctattc tttttctggt ctcctcttcg gccaatgccg gacctccctg
     6721 taacctggat caggaccttg ggcgcatggt cgggaaaaga ataacgtccc gaatggatct
     6781 ggaatcggtc agaaacattg taagccggtc catccctata ccgagccctc ccgtgggagg
     6841 aagcccgtat tcgagagcgt tgatgtagtc ttcgtcgaag gcgtgggcct cgtcgtcgcc
     6901 tgcttccttt ttcttcatct ggtccatgaa cctttcgcgc tgatcgagcg gatcgttaag
     6961 ttcactgaaa gcgttggcga cctccgatcc atagatatat agttcgaacc tgttcgtgaa
     7021 atcggggttt tcctggttct ttttggccag cggagaaacc tcggtgggaa gaccgatcac
     7081 gaaagtcggc tggacaagtt tttcctccac gaacgactcg aaaagttccg tgaggacaat
     7141 gaagcggctt tcgtgcccct caacttccag tcccttcttt ttggcaaaat cccgggcaga
     7201 tgcgtcatct gtcctatgga tatcaaaacc ggtgttctcc agaacaagtt ccgccatcgt
     7261 tgccctcctg aagggaggat tgaggcttat cggagttccc tggtagacaa tatcccggct
     7321 tcccaccgca tcggccgccg caacaacgag ttcttcggtc aggttcatga tgtcctcgta
     7381 attcgcgtat gcccagtaaa cttccatgga tgtaaactcc ggattatgca ttatatcaat
     7441 accctcgttg cggaagttct tgccgatctc atagacccga ccaagcattc cgacaataag
     7501 cctcttgaga tagagttccg gagcgatacg gagaaacatg tccactccaa gggcattatg
     7561 gtaggtgaca aatggccttg cgttagcccc cccggcgagg gtcgccagag taggtgtctc
     7621 cacctcgatg gatccgtgag attccagcac ttgcctgaag gaggatatta tcctggcccg
     7681 ttttctgaag gtgtctcgca catcagggtt ggctataaga tccacgtatc tctgccggta
     7741 gcgggtctcg gtatccttga gcccgtgcca cttttccgga agtggcctca gggccttggc
     7801 cagaaggacg cacctggtga ccagcactga aagctccccc cttctggttc tgaagggatg
     7861 gccctctatt ccgagcatat ctcctgtatc cacccatttt ttgaaaaatt cgtagggtcc
     7921 ctcacccatg gcatctacct ggaaatagag ctgcattccc gaggtttcat cagccaggtt
     7981 agcaaaggac gcctttccgt gttttctcag ggtcataagg cggccggcag taactataca
     8041 agtgtcatcc ctgtcgtcgg gctcgaggtg gctgaaattc tcccttacaa actcaagggt
     8101 gtgtttcttt atccactgct cgtgggcaaa gggatcgtaa ccctcctcct cccgaagcct
     8161 cctgagcttg tcggtcctct gcttcaggat ctcatcttcg gatacaacgt tttcgtcatt
     8221 agttctttca tcggtcaaga tgctcgcccc tttcggcaaa tactttccat tcttgaagta
     8281 tatcgtgcat ttccctccag tttgccgcca taccgacgga gcgacggaag agggaagctc
     8341 cgcgcaaccc cttaaagata cccgagagaa agcgcttgag caatagaagg gccgcccttt
     8401 caccctcttc ctcgacaatc agggctccca gttcctgcat catctctatc cttgaacccg
     8461 gttcacggga aagcagcttc ctgtcaacat caaatcccag ttcgtccagg atcaccggta
     8521 taaggaaagg gtccctgaaa gctcccctgg ccacgaaaac cgccgcgcag cctccctcaa
     8581 ggagctcggc agcatcacga gagcagaaaa catctccgct cccggctacc attccgggga
     8641 aggctgacgc tgtctcaatg atcacttccc tgtcggaaga acccccgtat cgctgaacgg
     8701 gcgatctgcc gtgtatgcat acctgggacg ctcccgcatc gagaagcatt ccagtaaaat
     8761 ccctggtgtc catcgggctg cctgagggca tcttcctggt cttgacccat acagggaggc
     8821 cgttcccgcg aagggacctg accatggatc cggcaatatc gggcctctca agaagtctgg
     8881 ctccagcacc cttcctgaga accttgggca tgggacaggc catgtttata cctatagcat
     8941 cgaaagaagg cgaggcggaa agggcaagac ccgccccctc gagaagagtt ttttcgtcac
     9001 ccgcaaaaag ctgaagcacc actggcctct caccgtcaag aatcctgagc atacggaagg
     9061 tcttgatgct tccatgaatc agccccgcgc agcttatcat ctcggtatgg gtcaaaccgg
     9121 ctccaagtct ccggaaaaag agtctcaccg aaggcacggt tatacctgcc agcggggcaa
     9181 gccataccgg attgtctacg gagacccctc caatattaat ttcgtcctgc cccacaattc
     9241 ctgtcatata taatccggag tccttcaagg gtcaaccagg gctcaactgc ggaaatggtc
     9301 ctcgaaagtg gggctatgac ttcggcatgc cccccggtag caatgaccgt tgccgaaaca
     9361 tcctgctcga gattcactct ttcgacaaga gaatccacca tccctgaaaa accgtagatc
     9421 attcccgact gtatggattc cctggttgtt ttgcctatga ctttttcagg aagatcaaaa
     9481 gagactttcg gcagttttgc cgcctttccg aaaagggcct ccatgctcaa tgccagaccc
     9541 ggggatatgg tacctccgag atattcaccc gaaggcgaaa tcacgtcgag agttatggcg
     9601 gtgccaaaat ccaccacaat gagcggtgac ccataccttt ttattccggc tacggcattt
     9661 acaaggcgat cggccccaac ttcggatggg gtgctgtaaa gaaccggtat acccagatca
     9721 gtttcactgg ttaccttgag acaatcaaga gaaaggtatc tgcctatacc ctcctcccac
     9781 ggagcatcaa gcgggggaac cacgctggaa agagccgccc cgtcgatatc ttcgggagta
     9841 attcccgcga gggagatgag gttaagaaga tatatcccca gttcatccgc cgtgtgtctt
     9901 tcggaaacaa ggcgccagtt tctgagcatc ttttctccgt cgaaaatacc cagaacggtg
     9961 ttggtattgc ctatgtcgat aacgagaagc atgttttcac ctccagagac tgccacctgt
    10021 tttggctaca ttaccatgaa aaagatagct attgcgccgg aaaaaagaac cgcataaaac
    10081 caaccgaatc cggtcccttt aagaacaaaa gaaagagcca ccgccatacc ggccgctgag
    10141 aacccggcag aacagggaac attccagggt agaaggaatg aaactccgtc cgccaatatt
    10201 gcccatgccc gaaaaagaat ctctcctgtc cacgcgaaaa aactccctcc cggaactccg
    10261 gccagagccg gaagcgcgag aagggacgca cctggcagga gaattccgaa aagagggacg
    10321 gcaataatgt taacgaaaat acccgctacc gggacactcc cgaaagtccc agaaaccaga
    10381 ccggcggtcg aaacccagag tatgaggggg gccaggatcc accccttccc gggtctggtt
    10441 ccctgaagtg aagaaaggac aagggcagcc gtcatagaaa gcctccagcc aagatcccag
    10501 aaaaaccagg gcctccagaa aagaagcaac aatccggcga cagaaaccat gttcaacgcc
    10561 tgaaggggtc gccccttcca tctggcgaaa aaaccggtct gcaacataag ccccgcgcgc
    10621 agggagcttg aggaagcccc tgcaaaaaga atatacagcc acatgaacaa ggacgatgtt
    10681 gcaagacgga aaaatctcac cggcaataac gtgaaaagaa gcaggacgac cacccccacg
    10741 tgaagaccgg acacggcaag tacgtgagaa gtcccccatg cggaatgcat gagcgcaagg
    10801 tcgggatccc tgtctcccag ccaggccgca agcaggtgtc ccctcatcat gggcggcatg
    10861 ctctcaagaa ttctctctct gacaagttca cgccatctcg agatcccgac cgacatttct
    10921 ccgctggacc gacctgatcc atgccggata attcccacgg cacccctggc cagccagtag
    10981 gtcccctcgt caaagcctcc ggaaccgggt tccggtgaga aaggttccaa aactccctct
    11041 acctctaaaa tttctccttc cctgaaaagg gaagagggca aaaggtggac aactattttc
    11101 ccgacagggg aatcaaggag aatgaccctc cctcttcccc aggaacgttc tctcacaacc
    11161 gttcctgatg tcgatatcct tatcggctcg aatctgtccg gttcgagcct ggataccatc
    11221 agaaccacac cgatagccgt aacagcgaca aggaagatga acacgtcccg gaagccttgc
    11281 ggacaataat gtgccgaacc cgcaaggcaa atgaaggcag agaccagagc acctccagcc
    11341 agggcgacaa agagagaggc cccggcggca cgcagccaga gagaaaggga acatcccgcg
    11401 aacaccacca gggcgggagc cactgctgca gggttcaacg ggaaactgtc accaggtcct
    11461 ttatacccga aagtttcgcc gctcctattc ccttcaccga gagcagatcc tccacgtttt
    11521 tgaatcttcc ctgctcctcc ctgcacctga ttatttcagc cgcggtcttg ggccctattc
    11581 ctggaagcga ctgaagttcc acggctcccg cgctgtttat gtcgatcagc ctggaaccgc
    11641 cttcctgcct gactccatta tccgtgcttc ccagagactg caactccccc ttgcggggta
    11701 cgtggatatg gatcccgtca tggaggggca aggccatgtt gaaggattcg ggatccgcat
    11761 ccgccgtcag gccgccggcc tgatccacgg cctggtagac cctggaccct tcaggcagcc
    11821 tgtagacccc gggcctgaag accgccccgg ttatatagat aacccattgc gtctcctcga
    11881 gagacccctc aacgggaatc tcccgtgagg tcgcatcatt tgctgttccg ggagctggac
    11941 ctgccacaac acccgatctc gttctttccc atctcccgga gaatgacgca acgatcgctc
    12001 ccgccgcgac aaggcagacc agacccgcga caatgaaaag aagcgctttc ctacggccag
    12061 ttgtattggg cattattgcg ttccctccgg tatgatctca cccctgagac tccagttgcc
    12121 agaaccggtt atcctgacca tcctgaaaga accaaggaga gaggcatcac ccggaaatat
    12181 gacaaccttg tccgcagaag tcctcccctg gagcatcgag tctccccggg gcgcaaaacc
    12241 gtctaccagc acctcgaact cgcgtccgac aagggcatcg tttattgcca tggtaatatc
    12301 ttcctggatc ctgtttatct ctgccagccg tctcttcttt tcggcaaaag ggacctgtcc
    12361 atccattcgg gccgcaggag taccatccct cggagaatag gcagccgtgt gaacaaggtc
    12421 gaatctgaat ttttccacta ccctgacgga ttccctgaaa tcatcgttgg tctctcccgg
    12481 aaaccccact ataaggtcac ttgtcaccga tggctccgga agatgatccc gtatcatctc
    12541 aatgatggat gcgtatttct caaccgtata gcccctgttc atcaaaccga ggatcctgtc
    12601 gctacccgac tgtatgggga ggtttatccc tgtgcagata gagggccttc tgctcatcac
    12661 ccgcactacg tcctcggtga ggtcctttgg atgagacgtg cagaagcgga tcctgttcac
    12721 tcctttcatg gaagccacgt catcaagtag ggacgaaaaa cgatagtcgt taccaaaatc
    12781 gtgtccgtat gtattgacgt tctgtccgat aagaatgatc tccctgactc cggaggagac
    12841 aagttcctgg acttcgctga aaataccctc cggatccctc gacatgaaac gccccctgac
    12901 gtggggaacg atacagtagg tgcaaaaatt atcacacccg tgagcgatag ttatgtatgc
    12961 cttccagggg ttttccctgt aaaggggaga tacgcccagg tcttcaagcg agaccgggtt
    13021 ctcatcgagt atggagagtc tctcccggga atccatgatc ctgtcgatac ccggaagaag
    13081 ccttccaaga tttctgggac cgcaaacgag ccttacagcc gggaaacggt cgagcaagga
    13141 atcgccgatc ctctgggcca tgcaccccac tacagcgatg accggctgcc cttcagcaga
    13201 ccgggatccg atccttccta tttcgctcag aactttctgc tccgcttttt cccgaatgct
    13261 gcaagtgacg aagatgacaa gggacgcaac atcttccaga acctcttccc accccgtcga
    13321 gacgagggct gtccttatcc tgtcagcgtc atagacattc atctggcatc cgtagacctt
    13381 tatggcgaaa gaccgcaatc ctttacctcc agtacgcaat ctgatctgct ttccagaccg
    13441 cctacggaat aataccactt tcgaggacgc agggtcatgg acaattcata acggttgact
    13501 gtaagatttt ccaggttctt cacgagaaag ggggcttcaa aaatggctcc agggcatttc
    13561 cttgccaggc tattcaacgt agtgactttg acaatgacat ttgcggcatt gctcgctgtc
    13621 tcttccccgg caacagataa cctcgctctg ccggagatcg aaggcttcag gtccgggaat
    13681 gctacagcaa ctatccttga taccccctcc ggaaaccagg gtacatggat ccagcaatcc
    13741 tatttccgga cacccgacaa tcattcagtc gatctgaaca ttctcatggg accaggaaca
    13801 gggtggcatg gactgcccga ggagggaata tcatcgacgg acggccccat cggttcagga
    13861 gccgtctaca tgaccctgag ggtatcgggt ttcccggctg cattggagat acatcccctc
    13921 acgggtaaaa ctctgacggt ctctccatcc ggccaggaaa ccctgacttt cgaaacaacc
    13981 ttcgaggaca tcgaccttgt ggaatttgcc gagaccttcc ttggtctttg tcaggtcggg
    14041 agcaatccct gaacctcagg ccagccccgg atcctgcctc ttgccctttt ctatagccac
    14101 agaaatagcc agggcggatg ccagactcag attgttgatg catggaaccg aagcctctat
    14161 ggcggccctc ctcatcctgt agccgtcgtt cagtacctcc aggttcccgg aagctatgtt
    14221 cactatcagg tcccattgcc tcgacgctat acctgagaca agagaatgac ccttatcgac
    14281 catttccgag ggaatacccc agttccccag aagggccgcg gtgccggcag tggcatatat
    14341 cttccatccg agagcataaa aagccgcagc aacatgggca gattctgtct tggccctgtc
    14401 tgccaccgac agaagcagcc tgcccttcac aggaactttc cagcccgcgc cacgaagtcc
    14461 ctcgtaaagg gcctcggcta cggtgctgcc cacccccaga gcctcccctg tggactgcat
    14521 ggccggcccc agaagggcat caactccggg tagtttttca gtcgagaaaa cagggacctt
    14581 gaccccaaaa ggacctttcc tttcctgaat tccggaacta taacccattt ctccgaaggt
    14641 ctcgccaaga gatgccctga cagccatctc caccagcggc atccctgtaa gctttcccgc
    14701 tatggggacg gttctgctgg ccctcgggtt agcctcgata acgtatatct cgtcccctga
    14761 aagcacgaac tggatgttta gaagtcccct tactcccaga ccaagtgcta tcttcttcga
    14821 aatttcctct atcatcttcc tcctggaagg agagagggaa acatcgggga aaatggccac
    14881 cgaatctccg gaatgcaccc ccgcggggtc aagatgctcg aaaattcccg gaataactac
    14941 ttcactgcca tcgcatacaa catctacctc aaactccgag ccgggaagga acctgtcgat
    15001 aaggacagac tggccctccc ctgacttgag cgccaggtca agaacggaac caagttccga
    15061 cgggccggcg gcgaccctca ttgcaaggcc gccgataaca aaactgggcc ttaccatcac
    15121 cgggtaacct atcatggagg ctttttccag cccctcccca acatcggaaa cggcaaaacc
    15181 ctgaggctgg agaatgccga ggctgtcgag aagcctcgaa aattctcccc tttcctcaga
    15241 agctctcacg accgatgagg gactcccgag tattttcacc ccttccggct ccagggcgag
    15301 accggtcttc agcgaggtct gtccaccgaa agcggcaaaa acccccgtga ccttttctct
    15361 ttccagaatg ggaagaatat cctcaacggc aagaggttcg aaatagagcg aatccgacat
    15421 gtcatggtcg gtgctgaccg tctcggggtt gttgttgatc atcacggccc gtttaccaca
    15481 tcgtcggata gcttctacgg ctttaacaca gcaatagtca aactccactc cctggccgat
    15541 ccgtataggg ccggaaccga taacagcgat ggcctcgccc gggtaatcgc gaaccccgtc
    15601 gtcaaggact ccacaactcc cgtaataata accggattcg gctggggttt cccccgcgca
    15661 accgtcgacc tccctgtatc ccctgactat cccgctggca attcttcggc tcctcagatc
    15721 accctcggga ataccaaggc aggaagcgat atccgcatcg gagataccca tctccttcag
    15781 tctccggaga agattttttt cgggacccct attcttaagt tcgtcaaagg caaggggaat
    15841 acgggaaaac tgatctacaa aaaaggggtg aatgcctgtc ctgtcggata cgacccctat
    15901 tgggactccc cggccaagga gttccaggat agcccagagc ctcctgtggg tcggaacgga
    15961 aagttcttca aggagatcgt cgtcgctcat gccttttacg cccggatcga aaagaccgcc
    16021 agggagatcc agagacctga cggccttcag agtggcctca atgaaggtcc tccctatggc
    16081 cataacttca ccggtggact tcatccttgt gcctacagtg tattctgctc cggggaactt
    16141 ctcgaagggc caccggggaa acttggctac aacgtaatcc accgcaggtt cagaaagagc
    16201 gctcccggac ttagtcaccg ggttaggcat ctccgtaagc aggtcgccca gagcaattct
    16261 ggccgctata cgcgcaatcg ggtaccctgt ggccttgctc gcgagagcgc tggatcgact
    16321 ggccctggga ttaacctcta tcaccgcgta ttctccggca tccggggata ttgtgaactg
    16381 gacgttgcag gctcctctta tgtcaagttc atcgactatc ctgaaagcag aggaacggag
    16441 gcgctgccac tgcctgtccg aaagggtaag gaccggagca actacgatgc tgtcacccgt
    16501 atgaattccc atggggtcaa cgttttccat gccgcatacg caaagcctgt ttcccgcgca
    16561 gtccctgact acttccagtt ctatctccct ccagccctga aggtatcgct ccaccaggac
    16621 ccttgaaacg ggagaaaggc gaaggccctc accgaccctt tccctgaggg acgccgcatc
    16681 gaacgcaacc cctccgccag ttcctccaag ggtatagtca ggccttatta tgagcgggaa
    16741 ggatgttttt tcggcaaagg caaaggcatc atcgatgttg tcgacgtatg cgctctcaat
    16801 tacaggctca cctattccga ccattgcctg gcgaaaatct tcccttccct cggacctctg
    16861 gacggactcg ggtgacgtgc ccaggacctt tacaccataa cgctcccaga ttcccttttt
    16921 ctgcaattca acacacaggt tcagggccac ctgccctcca agagttgcaa tgaccccgcc
    16981 gggcctgtgt gtccttatta tctcctcaac aacatccgtc tccaggggcc ttatgtaaac
    17041 cacatcggcc atggccgtat cggtctggat agtcgcggga ttgctgttta aaaggaccga
    17101 gtgataaccc tggtccctga gagccctgca ggcctggctc ccggagtagt caaactcggc
    17161 cgcctgacct attcgaatag gtcctgagcc aaggacaagt atcggtttcc tgccagtctt
    17221 catcgggccg aactcctttc gcacatcgac agaaactctt cgaaaaatac ctctccgtca
    17281 gcaggtcccg gcgaaccctc cgggtgatac tggatcgcca ggacctctcc acccgggctt
    17341 cttacaccct ccacacttcc atcggaaaga ttacggtggg tcacctcaag ccccgtcccc
    17401 ttcaggctgc tctccgagac ggcgtatcca tggttctgac tggtgaccag tcctcttccg
    17461 cttctcagat cgataacagc atggttctcc ccacggtgcc cgaagggaag tttataagta
    17521 gacgcaccgc aggcaagggc tattatctgg aggcccagac atatcccccc tatggggacc
    17581 tctttcagca gcaaggagac gacctccgtc tcttttacca atgcagaagg gtcgcccgga
    17641 ccattgccga ggaggatccc gtcggggtgc caggaaagga tatcttcttt tgaggaatta
    17701 tggggaagcc tcacaaccct gcagcctcgt ttcaggagct ccctctggat gccctgcttg
    17761 agaccgtaat cgatcacggc aatggtcttt cccggtccat ccatcaattc gggagccttg
    17821 atcgagacct cttcgacggg atgagttgag gtcatcccga aaaccggagt ttctccggaa
    17881 ggagcgacgg tcccctgaag ggctcctttt gtcctaatat gccttacgag ggacctggtg
    17941 tccacaccgg tgaccagcgg gatatcgtac ccggcaagcc actcttcaag acgcggccca
    18001 aggtaatccc cggcctctac ggctcccgcc agaacggccc ttgcccaggg tcggccgctc
    18061 tccagaaaac actcatccac accgtagttc cccaccatgg ggaaagcgaa tactattatc
    18121 tggcccgcaa aagatggatc gctaatggcc tgggggtaac cggaagaggc agtagtaaaa
    18181 acaacctcgc ccgacgcaaa accgccatgg cctgtcccgg accagaaact gccgtcttca
    18241 agaactatct tcatctcttt cataaacttt cctcccgggg aataataaag aagggttcat
    18301 aatgactata aaaaacaaac ctgcgttcag gtctgaacga ttatatcctg caaaagggaa
    18361 atgtcaacaa aaaggggggg ccgaagaggc cccccctttt atttctatgg atctgttccc
    18421 cgagccgata cctgtgtttt tagtcaacga cctcgaacat atcctttcca acaccgcata
    18481 cggggcaaac ccacccatcg gggagatcat caaaggagac acccgccgca actcccccgt
    18541 caggatcgcc aaccgcaggg tcgtattcgt atccgcaaac ggtacacagg tacttcttca
    18601 tcgcaacttc ccccttttgg cttttcagtg tattgctgct ttttccaaga ttataagatc
    18661 tcgcaaagga aatgacaacg cccgggactc attcggttct ctgacccaag ggaagaatcg
    18721 tactcaaaag agacgaggta agacattctc ttccttcggc agtgatgtaa aaatcatcct
    18781 cgagcctcat gccacccttc cctgggaaat agattccggg ttctacggta actacatccc
    18841 cctcggcaag aatgtcttcg gagcggaatg aaagaaaggg agcctcatga acttcgagac
    18901 cgatcccgtg tcccaggcta tgactgaaag catccccaaa acctgctttc tcgatcacgc
    18961 tcctcgccgc catatcgaca tctctggcgg gcctgcccgg ctcaagaagc gctatggcgg
    19021 ccgactgggc ctccgtcagc agttcatggg cctccacagc ccattcagga ggttctccat
    19081 cacaaaaaga cctggttata tccgagatgt acccgttgaa cctggaaccg aagtctatgg
    19141 tgatcatctc acccggctcg atctttcttc ccgtaggggt tccgtgggga agggcgctcc
    19201 gtggccctga cgccacgatg aactcatggc ttccccatcc cccttcggca ccggccatcc
    19261 ggatattgta ttccagcctg gccgcaaagg aactttcggt taaatctttt tcgccttcgg
    19321 aaagggtctt cagaagggca gaagaagcaa ggtcggcggc gacgcggata tctccgatct
    19381 cgcccccgtc ctttttcctt cggagcctgg aaaaaagagg ggacacatcg acggtacttt
    19441 taccgaatcc ccttattttc cccatgaggt cacaggttac ccggtcagcc tggaaaccga
    19501 tggtccctat ccccccgata ccggaaatga tatcccccag gacatccatc atgggggtcg
    19561 atgcctggtc tataaccggg atgcgagtct gcgaagcagc ctgggcaaga tatcttccat
    19621 cagttatcag ccccgcctga tcactggtcg cgtaaaaaac cgaactggaa cctctgaacc
    19681 ccgtcaggta gaaacaggtt tcccagttca gcccttccct tacaggaact attaccgcgt
    19741 caaccccggc gctcttcatg gaggatcgca tggcttctat cctgcgttcg gtctgcatgg
    19801 ccctgttcat ttccatcggc aaccctccgc gagaaggcgc cagagttctt caaccgtctc
    19861 ggtccccact gatccaggac cttctgtaat actacctgcc accgcgcttt tgatacccgc
    19921 cttctcgaga gatactctgg cttcttccag atgttcccgc gggatcaccg ctacaagaac
    19981 tcccgaagat atcaggcgca agggatcaaa acccaggcat gtcgagcaat gccttgtaag
    20041 gggatggatc tctatagacg aagtatcgag atcgatcccg aaaccgctga gacggcagat
    20101 ctcaccaaga cctcccagga atccgccctc ggtagggtca tgcatgaaag aagcccactt
    20161 cctgagtatc ctcgactcgg gcaaaacatc gaggagcgaa ccccagccca ggatctcgtc
    20221 aatctcggct tcatcaaggc atttcccgag gtattggcgg cagtctccgg caaggatgca
    20281 catgccctct atcccgacgt gtttcgtcat cagcactaca tcaccgggcc ggacatccct
    20341 ggccgatatc accctttccg ccatacctat catggtcgcg caaagaacag ggtgagcgta
    20401 tttgtcgttt atctcagtat gacccccgac cactgatatg cccgcttcga ggcatgcctc
    20461 atgtatttcc tgcacaacgg aagagacact ttcgggagtc gtcccggccg gaaatatcag
    20521 ggtacttatc aaataagcag ggtcggcccc ttttgaagca atatcattgg aattaacggt
    20581 cacgagaagt ctgccggctc cactgctggc cccgactatg ggatcggaag ccacaacaag
    20641 gtatttgccg accggccagt cgattaccgc tgcgtcttca cccactcccg gtcctatcag
    20701 gacctctttt ctcctcgctc cgccgtaggc tagaaccgct ttttccagaa tttccggcgc
    20761 cagctttccg gaaactgcgg tattctgttc tcccttcact gcagcatcac tccgttcctt
    20821 cgtcccttat gctcatgacg cagccgcaat aattctgtcg gtacaatccc aactgcctgc
    20881 ttttgcggac cgaagcggga aaccccccgt tcttcctcca gatcctggac agccattcga
    20941 gaccccgttc atcacaaacc tcacgaccaa tgacgtcgac aagaccgaca tccttgtgag
    21001 ggcttattgt cagtgtcgtg cataaagcat caaagcctct ttcaacagcc attcctgccg
    21061 ccccttcaag ttgttccctg aagcaaagcg cgcacctttc cccgccctcc ggcgaaagta
    21121 tatgagaaga tactttttca agccagttca ggggcctgta ttcatcaacc agaaactcgg
    21181 ctccaagaag caatgcaagc ctttcgacgt cttccgaccg ccttcgaaat tcttcgaagg
    21241 ggtggatatt cgaagaataa aagtaccccg tcactttatg accctcttcc ataagagcag
    21301 gccatggtat cgtcgcgtcg ggggcacagc acacatggag gacaactttc aaggtcaaat
    21361 aaggtcaccc gctccttccc cttccacctc aggcaacgcc tcgacgctcg aggcaaagac
    21421 aaggtatccc gtgtgtccta tcatccggtc ctcggggcga atcctcctgc tgtttacctt
    21481 gtaatgtctc agcattattt caacgatctg gatgtcggca aaggaattgg tctccagtcc
    21541 atcaatcacc ttctcaagct ggttggtcgt agggacaagg atacccagcc tgttgccggg
    21601 cgcgagagtg gccttggtct gttcgagata gtcccagggg ttgggtaggt caagaaaaag
    21661 cgcatcggca tttcgttcgt cgaaaccgtc ggcaatatcc cgcaccttga actctaccct
    21721 gtgggcgatt tcccatttct cgcagtttct tttcgccagg agtgagaatt cttctcttct
    21781 ctcgtatgaa acgaccttgc cctcatcacc cacaaagtga gccagaacgg aagtgaggct
    21841 tccagaacct gtcccgcatt ccaccaccag tgccccgggg aaaatattga gagagacaag
    21901 gataaaaccg atatccttgg gataaagtat ctgggtctgg cgccttaccc tcctgctgaa
    21961 ttcctgaaca ttgggtctga gcatgcagaa cttttttccg agattggtag aaatccagcc
    22021 cccataggat ttgcctatca tattatcgtg aagtatctgt ccaagacggc tgtcctgcct
    22081 cccgccaggt acgatcctga cgatgaaact gtcacctttt ggcggagccc agataaaaac
    22141 aaggtctcca ggagcgatca ggttatccat atcgtctctc ctcgcgttaa cacattcgtt
    22201 atgcaggggt gttccggatc tcatccctga aagtctcgta tatctccgcc atccggtggt
    22261 ggaattttct gaaaatgtcg agaagcgcag ggtcgaagtg ttcgggcctc agccgctggt
    22321 cgccctgaag aatgattctt actgtttcaa ggtgtgtata tggtggtttg taagatcttg
    22381 aagagcgcag ggcatcgtag gtatcagcga gggccactat tctggcctcc cagggaatgc
    22441 tttctccgga aagcccctcc gggtaaccgc ttccgtccca tttttcgtga tgatagcggc
    22501 agatgttcct ccccatctgg agccacgagg cttcgcccag aatctccgcg ccccattctg
    22561 tgtggtgttt gacaatatcg aactcggcct tgttcaaccc tgccggtttg gaaaggatct
    22621 ccatggggac cttcagtttg ccaatgtcat ggagccttga gaagagggct acatcctcca
    22681 cctctatcat actccgagac atcgctacag acagaaaacg acagtaatgt tccattcttt
    22741 ccagatgggc agcggtctcg tcgtggtaaa ttgcagtgag gttctggagc ctcccggcca
    22801 tgtatttgta ggaatttttc agatccatca tggatgcctg ggtattgaga agtccggcaa
    22861 catggagggc cacaggcatt accagttcca caagggcctt atcctcagtc ttgacctggt
    22921 tccacgatat cgcaagacct ccgacggcct tgccaatatg gaacatgggt atattcagtt
    22981 cgcttttgat cttgcgatta agttgacagt aggaccgatc ggaataaaca ttctcgaccc
    23041 agacgggaga acctgaatcc aaagccctgc cgattatgct tccatcaaga agggtatcct
    23101 ttatctccag caatccgtca aatccgctcc aggcaagaac cactattctg tcgccctcaa
    23161 tcctgcagat cgccactcca taagcgccag caacatcggc cagtatcgat gcgacccttt
    23221 cgaggccttt ctggaaaccc gactcgatgg tgacgctcct tgacacatca aggatctttc
    23281 tccatatctc gtcgcgtctc agcaatcccc tgttggcctc cgagagagca cggttcgccc
    23341 tctccagttc cttaccgctg tccataatct tttcctgctg gtaaacatac ccttgaaggg
    23401 cacttgagag ggtctggcaa aataccttta cctccgaaag cctgctgttt ctggtaaggt
    23461 cccttatacc gccggcaagg ttcgccacgc cttcagcgag atcagcggga ctggtggatg
    23521 taaaaagtct gtcctgcatc tggagcgaaa gcctggttac tctctcgaga gggtcaagaa
    23581 gaaatctcga aaaaatgatc catgccgcaa atagtcccat tattagaagc agctccaccc
    23641 ctattgtcca tggaagcctg gcaagggctg aaaggacaaa aggcacggcg gggaagacga
    23701 cctcaacaga aaggcctccc ggaccgatat cagcccttct tttgtaaacc ggggaccata
    23761 ttgcgtcact cggggtggat atacccgatc cagccttccc gggtaacgaa aggggctgtc
    23821 ctgtgcgcca caacaggctg ccgtcggccg ctactatcct taattccgct ccgtccatac
    23881 caggcaagag aaagtcggcc caggaaaggt ccggcctgta tttaataaag gcagcgggat
    23941 aaccttccgg agcccttctc gcaaatgcaa gttcaccctg accctgttca accgaaagaa
    24001 catgccaatt gccgtgcatt ggcagtggtc tggacaggcg ggtctcgtta ggaacaaggg
    24061 ccagtggtcc ggcacctttg ggcagcaaat cccgcgatgc ggacacaccg gtatcggtcg
    24121 atactatccg gtcgatctcc tggaggtcgt ttcctaccac aaaggatatt gtcctcgttg
    24181 ccagaacgag ggcagaatcc aggttcttta tctcggagcg tattcccagc gagacactga
    24241 aacccaggtg gacgaatatc agcgcgaaca aagccgcaat cattatgatg aaaagatttt
    24301 ttgctttcat ggggtcgacc atttcacaac cttgagatgt ccgtattcaa atccttgtta
    24361 agtattctac agcaaaaaaa tggatacaaa aaagggggag ctcccgaaaa gctccccctt
    24421 tttgagttct gactcaaaaa aatctagaaa cgccgctccc agctcagttt tatgccacgc
    24481 tcggggccgt agtagtatga gccgaacccc gcctcaaaaa tattttcttt accaaataca
    24541 ttgatcaagt tcagacgaac cgtatccacc tcagactccc atgaaagggc aagatcaacc
    24601 tgggaaaggt gatccgaatc cctgtttccc tggttcccgg accagctatg aatcagtaga
    24661 gctgtccatg gaccgctctt gtactctaag gatcccccta tctcccactc cggaacggca
    24721 gtgcttcgaa cccagtcgga ggtcgctgtc gctttctcct ccggcatctg ccatgtcccg
    24781 ttgagcccca gagtccagtt cgtcccaagt ttccattttt tagttgcctc aatgccgtag
    24841 gttctaaact cggcaacgtt gatataggta gaagggaaag ggaaacgata atcatatttt
    24901 attttttcat ccagaactgt aaggaaaacg ccgatctgcc agggctccaa caaatcaata
    24961 cccttgagac caagttcgta actccacccc ttctccggct ttaaatccgg gttgccaact
    25021 gttttatctc cccatcccat gtcataatct gcaaataaca tataaaaagt gggcatcgca
    25081 aacaccctgg aggcagagat gtagatcata ttcccgttgg caaactgtct ctggagactc
    25141 agtttcggga tcagctcatc gtgatcgttt gaatcttgcc tccatatttc gtacctgagg
    25201 ccaaggttcg ccacccattc gccaagggga taagacgcct cgataaaggg cgccaggttg
    25261 taccggtctt ttgagaccac agggttgacc gctccatcat ccttgaattc tgtgtcctca
    25321 cggcgataat acaaccccca ggcaacgagg gcttcgccaa gggcactctt tccgcccact
    25381 tccgcggtat acgcactgtc ctcaaaatag ttattcgggt tctgtagaat atcgtaacgc
    25441 tgggtgtcat accctgtaag aaggtaccag ttgtcccagt cgtaacggaa ggagtagcgg
    25501 ctgtaggtct tcttttcatc gttgaaagcc gtccagttcc cctcatactg atacttgtaa
    25561 tccccccatg aggccttaaa aatccaatgt tttccactca aattcaggcc ataagcatca
    25621 cctttataac caagggaatc atacctgttg gttggtgaag tatcatagaa aagcctcttt
    25681 cgtccctctt cgaccctttc gtaccagatg ccggcgttga ggacatctcc cccggtgtta
    25741 cccgacacat aatacctgcg ccagccgctg ggccccgcct cggcgacgac cttgccgcca
    25801 ggcttttccg gcttcttcgt gatgatattg atgacgcctc ccgcagccat ggagccgtaa
    25861 atggcggaag cgcctccctt tacgacctcg atccgttcga tatcctccag tgcgaaagac
    25921 cggaaatcaa cagccccgaa gtcaactcct atcgaagaac tgtaaagtgg aatcccgttg
    25981 ctcagaacaa gcacctggtt ggtgaaaccc ctgaaccata tctgggactg ctgggccacc
    26041 ccgctcttct tcttcatgaa gatgcccggt atcctgctat ccagaagcgc cccgagatcc
    26101 gacgccccgc tggcagcgat atcttccgca gtgatcacgt acgcaggtgc cggaacctca
    26161 ttcaggtcgg tgtagatcct gctccccgta accacctccg gcgccaccgt cgtgacctcg
    26221 gccgaaacac catgataccc cgcaagtaca aatgcgatcg cagcaaaaac agtaaaacca
    26281 acaccaaaaa cctttctcat caagccgtcc ctcccaaatg aaatagggcg gcctgacacg
    26341 ccaggccgcc cagcaaaagg acggatattc tgccgtgtca cgcccgcgca tgacacatgg
    26401 acttcgcccc ccggccggtc tcctggcttc cgttctccct actcccgcac cttcccggca
    26461 ttcgccagtg gttcgatacg cggtttcgtc acggtaacag tggcggggcc gctccggtct
    26521 ttcaccggaa ttcccgacac cgaagggttt ttccatatga tccaacaatg aaaaggatac
    26581 accagaaaaa aagaggggtc aatcaggggc aagggcttga ctcgtaactc gaaaaaccag
    26641 attcttcgtc atttcattcc tctgaatgac aaagaaaagc aaggagactg gcatctggag
    26701 gcaggagacg ggaagggcaa ggccaagggc cggagagaag tctttgccga aggctcagga
    26761 gccggtgagt ttcgcagccg tcgcggaggg g
//
LOCUS       DAOTIQ010000020        25286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2277_length_25286_cov_6.718402, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000020 DAOTIQ010000000
VERSION     DAOTIQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 25286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25286
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2277_length_25286_cov_6.718402"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..815)
                     /locus_tag="P5541_04220"
     CDS             complement(51..815)
                     /locus_tag="P5541_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71218.1"
                     /translation="MRSSRFATAVFLAILFIAMPVYAEVSKEFKGEVEWTTSGERLFA
                     ALAERLDPDSMEMVLDEEPGEDGSLRHMYLLVKGARFGAFRLEEMALETRFGKFNPVS
                     EWVDAESIKVEEILSGNFAAIVLEGDINSALREQIDENWQNVRVEIRPGGIFARGYYV
                     VRGSISLKILVELSTALEVRSRKVWLKDYSLFVNNAEKTSIVEQAVRDLQPVVDLEDF
                     VFPLNIDSLDLGGNSVKLSTRTPPQIFEGIRYLYSR"
     gene            complement(845..2659)
                     /locus_tag="P5541_04225"
     CDS             complement(845..2659)
                     /locus_tag="P5541_04225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HRV71219.1"
                     /translation="MGDSPKGRAGTFSSQALLPALPVPDLEETIERLLEWASPLLDPD
                     TLERTGRSAREFASESGPGPILQKALKEFAARKDVANWLEPFWEWMYLSGRASLPFYS
                     NIFYMFSPRSASPKEITQAGHAAGLVVSALRFKKLVDTGQLPPDTEKGRPICMIQYKK
                     MFSSARIPEKGMDRIRNPVISHDPTEPYERHIVVISRGRYFSVDVIDHEGNFFDVVAV
                     ESAIQEIMDLEGEATSPAGVLTTLPRDDWAEARANLSIISGNRKILDTIERALFVLCL
                     EQGQPEGIKQLSETMLTGDGRSRWFDKGMQFIVCPDGTSAINMEHTATDGSVMVRFAS
                     FVGNDDPWNGEGSINNGKCPWRDLVFDIDDDMASIIESAATAFDRMKRDTETEVLIFG
                     SFGKERIKSLSVAPDAFVQMAMQLAQQDVWGVPRSTYEAVMTRQFLHGRTEAVRSVSE
                     ESVIFTKVMRDPSATDPEKAVAIRAAAEKHVSRMKESKAGKGVERHLLGLRRIFETMG
                     KDLGLQGEPEFFSDRGLKTLTRTLLSTSTSAVDGMYLAGFGTVDDEGLGVRYLTFADS
                     IHFNITSRTALRGQMELFAKKLESALGEMAETIDRTKQ"
     gene            complement(2695..3435)
                     /locus_tag="P5541_04230"
     CDS             complement(2695..3435)
                     /locus_tag="P5541_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRV71220.1"
                     /translation="MTVYHVRSRTRSWDGESIGILILDACYPCVPGNVGNATTYNFPV
                     RYREVRGASIERLLNERDPSLLEPFVEAARDLEAEGVRAVTGACGFMALFQRQVARSV
                     KIPVFLSSLLQVPFIFRTLAPGRKVGIITANRSCLTEEHFESVGITSEMPLAIYGMED
                     QEEFRTSILEEKGTLDSDLIRIEVTDVARRMVRENPEVGAVLLECSDLPPYAAAVQEA
                     VDLPVFDFITMINHVHSAIRRTPYVGQM"
     gene            complement(3470..4096)
                     /locus_tag="P5541_04235"
     CDS             complement(3470..4096)
                     /locus_tag="P5541_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71221.1"
                     /translation="MKRVMLLSLLLFVMLAGAACVEAYGEWLESGTLPDGPDQQNVDP
                     PLWGAWFLPGQVWMREAVGGQESSGPRFFCDVKGEWYFLEDGTEGVPVPCEPPGWYPW
                     QDTPEKRGLTSKGKEESAWEKNPSFSWGEWENHFGGWFRRVNPPGPPLLGASHMVDEK
                     GDCWVLVVYPGATPRRVERFSWFPGFPPGHVIRPETTVLVKPETGQAQ"
     gene            complement(4371..4447)
                     /locus_tag="P5541_04240"
     tRNA            complement(4371..4447)
                     /locus_tag="P5541_04240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4411..4413),aa:Arg,seq:ccg)
     gene            complement(4760..>4984)
                     /locus_tag="P5541_04245"
     CDS             complement(4760..>4984)
                     /locus_tag="P5541_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRV71222.1"
                     /translation="RGMTLDEAMPEVEVYLDRAFRAGYGEVTVIHGRGEGVLRRAVHS
                     LCEDLPYVVDQRLGNAGEGGFGVTIVSFRR"
     assembly_gap    4985..5084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5085..6934)
                     /locus_tag="P5541_04250"
     CDS             complement(<5085..6934)
                     /locus_tag="P5541_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRV71223.1"
                     /translation="MKTEPKTPQLVEIPAVLERLARKTRSENGQQAFLSINPCHTQKQ
                     AIHRQSLLRAYMRYVAKKGDFPWDGSLRPVSPFLETAKQSSYLTGEELLRFRTLIGLA
                     IKIRLAANEAKGDIPGIEGLAEGIRDMSKELSALQVIADDGELYDQASPKLSKVRGEL
                     RDTLSRARSRCMDIGGQQQFGPYLQDRVVHLRKGRLALLVRAEDASRFDGIVLDRSGS
                     GKGVYMEPREVVTLNNRAEILRGEEKEEERNILSGLTAMILEKKSAILDAQKAVAFID
                     ILHACSIILDESGWSLPEIREKPGFYFRELKNPLIASNCVPITIHCGDRFRQLVITGP
                     NTGGKTVVLKTVAIAVFLALSGLPVPAAEGSVAGWVDFMAADIGDEQGIEQSLSTFSS
                     HVSRIVMMIEKSGKDSLLFLDELGAGTDPQEGAALGVAILEKLREKRCLVLATTHHNP
                     IKKYAVTSKGVESASMEFDPVTFSPTYRLLMGVPGKSNAIMIARRLGMSEDVIDQAMQ
                     NLSGESRSAENLMAELFDKQVELENAAREAAEERRKLAEMERLYSEQVDSMAKREESI
                     LLAADSRARGILEDAEKAAREMLKNLEGAAESAARREFEKGRKETSELKK"
     gene            complement(6931..7494)
                     /locus_tag="P5541_04255"
     CDS             complement(6931..7494)
                     /locus_tag="P5541_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRV71224.1"
                     /translation="MTLSGGVDLLFLLILVTFCIRGGLRGLSGEAISLVTTIGGFILA
                     WKVSPQVSKLVSPLIPMSPAAVQTASIVAVYVTVLILGVFAGRVIKAFLKFTNLSGVD
                     RGMGIVAGAVKTYALLMIVYAGVLYFSPVISPYWMNESYSMTLAHRSWPMIQKSLSGV
                     RLLEPEALPWSGAPSETGNGKDGNSTN"
     gene            complement(7491..7733)
                     /locus_tag="P5541_04260"
     CDS             complement(7491..7733)
                     /locus_tag="P5541_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71225.1"
                     /translation="MTDEPRRVYVTLGKKSYSILTLLDEKRFERVARIARDSLSGIDI
                     SIDQEDRLLLACFKLAYSIENAENRLRKISGESDGS"
     gene            complement(7753..8094)
                     /locus_tag="P5541_04265"
     CDS             complement(7753..8094)
                     /locus_tag="P5541_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71226.1"
                     /translation="MFEHMENLERLIDGVSSTIQTLRDEKSQMAEELDVVREVLEERE
                     KELASLVEERTALERRLEAILEKARSSFGFSGDGGDPFQEALRTDGERLPDEDDGSED
                     SPRQGELTDRF"
     gene            complement(8116..10503)
                     /gene="pheT"
                     /locus_tag="P5541_04270"
     CDS             complement(8116..10503)
                     /gene="pheT"
                     /locus_tag="P5541_04270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRV71227.1"
                     /translation="MLVSLNWLRELVDIPLDTEALARRLTDTGSEVESISTPVPLFRG
                     ALTSRVSRISVHPSRPELFLLEVEAGESKGVCVTAARNLHEGDTVLWGPPGCVLASGD
                     VIGTRDFEGVISEGMVLSAEEIGMPEISDEFGILRLGDGYSPGMDAREALGLDDTVLE
                     LSITPNRGDLLSMMGVAREVFAVVPGAVFRVKDPVIPEESESLEGFSGITLLDPGCPF
                     YALGSIDDIRIAPSPVSTRIRLLLSGMRPVSNVVDATNIVMLLTGQPLHAFDASGLPG
                     GEITVRSASSGEILQTLDGKARVLDGSDLLITSGGKAVALAGVMGGSESEIGPETGRV
                     LLESAHFDSIRVSRTSRRLGLPSEAAYRFSRYVDPFKVKPALAMVMDMISEWGGGTPG
                     GWISSGKPSGSGRNASLSSALLDKIINSDDLEMATAILDRLGFVDEGGSSSQRSYIVP
                     SWRPDITIEEDLIEEVARIRGYDGIAPALPPVMHTTGDITDRMRAERSIRGTAMGRGY
                     AEVITYSFISPDFGEKFRIPPEDRRSRAVALSNPLTLDNSVLRTTLAPGLVKALKENI
                     RSGWRGPVRMFEVGAVFFPEGEEVEEHLKLCGCVCPGKDTRSPWGAQAVEDFHSVASD
                     LEAICAGRGIWIELVRGEEPFGHAGKTARVIADGKDAGYILSLKPEIEAEQDIPGPVY
                     LFEIDMDILIEGGLANFGEARRYPPVYRDISLLVKDGEYSAEVLELLRNLADPLLEKA
                     WLFDVYSGKGIPDGYRSLAFSLAYRDPDRTLRDDEVDLIHERLRTLLKEKGIVLR"
     gene            complement(10503..11546)
                     /gene="pheS"
                     /locus_tag="P5541_04275"
     CDS             complement(10503..11546)
                     /gene="pheS"
                     /locus_tag="P5541_04275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRV71228.1"
                     /translation="MNKISDDLDRIRLLFREELSKAFTREEIQSLRVRFLGKKGELTS
                     LLKRLGSLPAEERPEAGKVLNELRDEMEKDLSARETERAGQDLAARETKEWIDVTLPG
                     RGRPWGAFHPVAQVTQEAIDIFASMGFSVAYGPEVEDDFHNFEGLNIPPHHPARDMQD
                     TFYLENGLLLRTHTSPVEVRSMRKWGAPLRIIIPGKVFRRDSDQTHSPMFHQLEGLLI
                     DENISVAHLKGCLEAFVNRIFGRPLAARYRASYFPFTEPSLEMDVECVACSGKDPSCR
                     ICKGTGWLEISGLGMTHPNVLRAGGIDPVRYNGFAWGMGLDRIAMLKYGLKDLRVFFE
                     GDVSFLTSTGGRR"
     gene            complement(11530..12234)
                     /locus_tag="P5541_04280"
     CDS             complement(11530..12234)
                     /locus_tag="P5541_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRV71229.1"
                     /translation="MSEKTRTCIVLGLGRFGEAICLRLAELGQSVIGVDRNRSRVEAL
                     SETIDLTAQLDVTDEAALEKVGVKDCDVAVVAIGESLEASVLASAILVGFKVPLVVAR
                     ARNALHARVLARVGVQRVVFPERDLGLRTAEQIVHPHLSRFSEIPGSGLIVGEVGALP
                     EMFGKTLQELDFRHQYNAVVLLVNRNGDRFLPRADTVLEDNDLLMVTGKQEDIGCWID
                     RCKEKVQGGETLEQNI"
     gene            complement(12250..13572)
                     /locus_tag="P5541_04285"
     CDS             complement(12250..13572)
                     /locus_tag="P5541_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRV71230.1"
                     /translation="MRTFRSFGIERTIIFGFLSLILAGTLALWISSHFFDPLGWVDSL
                     FTATSAVCVTGLVVVDTGSDLSLISQYILLFLIQLGGLGVMTAATALSLVWGRRIGIR
                     QRLLIKGGFGLDTPQGAVRLILWVVRFTIVIEMAGALVLFAGFLGTHGISRAAYMAVF
                     HSISAFCNAGFSPISDSLMGYQATWMVPGAVMSLVFFGGLGFIPLLEIWSSFRGGRIR
                     PLSVHTRLVLSVSLWLVAIGAALFAVSEWTGTLEGLTFAFRIWNSLFHSITPRTAGFN
                     TLDTSSLSSAGILLTCILMIIGASPGSTGGGIKTTSLGVLLAVTSSELRGGGEVTLWN
                     RRIPSRIIKRALSLLVVYLGTIIAGTGLLLVFEPFGLRDVLFEVISALGTVGLSTGIT
                     PHLTAPGKLVLVILMFWGRVGLITFMYALVDHAPTARVSYTDTNIPVG"
     gene            complement(13575..>13835)
                     /gene="rplT"
                     /locus_tag="P5541_04290"
     CDS             complement(13575..>13835)
                     /gene="rplT"
                     /locus_tag="P5541_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRV71231.1"
                     /translation="RAQEAYLHSMANAYRDRRAKKRNFRKLWITRISAGARMNQMTYS
                     SFMNGLKCANIEINRKVLADLAIQDMPAFARLVDQAREALAR"
     assembly_gap    13836..13935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13936..14024)
                     /locus_tag="P5541_04295"
     CDS             complement(<13936..14024)
                     /locus_tag="P5541_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HRV71232.1"
                     /translation="MPKMKTHSGTKKRFSTTGTGKFVFKKSGRG"
     gene            complement(14061..14666)
                     /gene="infC"
                     /locus_tag="P5541_04300"
     CDS             complement(14061..14666)
                     /gene="infC"
                     /locus_tag="P5541_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRV71233.1"
                     /translation="MAKKSDEPRINEEIRVQEVLLIDDQGVKVGVVPTSRALALAEER
                     ELDLVEVSGQADPPVCRILDYGKFKFQQQKRDKDARKKTKGQVVKEMKMRPKIDLHDY
                     NFKVRAVKDFLLSGFRVKVAVFFRGREMAFLDRGREVLDKVVRDCEGIGKIDMPPRME
                     GRYMRLMLTPVPQTQKKSRQPADEATEQVSSAGETSATEDN"
     gene            complement(14877..16796)
                     /gene="thrS"
                     /locus_tag="P5541_04305"
     CDS             complement(14877..16796)
                     /gene="thrS"
                     /locus_tag="P5541_04305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRV71234.1"
                     /translation="MPEYRGPEDRILESEKILKAGEILKKWGISGDSVAAMVNGVVAD
                     LSTEVKGNSTIEPVRAGSPEGLDMIRHSVSHLMAQAVQRLFPGTRLGIGPSIQDGFYY
                     DMEIKGTISEEDLPRIEEEMNRISKESLPVERVFLPRKEAENFFRDRDAVYKLELLEG
                     IEDDEISIYRQGEFADICRGPHVSSTSDLKHFRLLSIAGAYWRGDEKNIMLTRIYGTA
                     FDSAEALERYVNRLEEARRRDHRKIGRDLDLFSIHNEGPGFPFFHPRGMVVINKLVDF
                     WRAEHIRRGYSEIRTPLILDQELWIRSGHWDHYRENMYFTEIDERPFAVKPMNCPGGI
                     LVYKTKIRSYRDFPLRIAELGTVHRHERSGVLHGLMRVRCFTQDDAHLYVTPEQIKQE
                     IIGIMDLVRFIYRDVFGFPYRVELSTRPEKAMGDPAMWDTAEKALKEALEEFGIEYRI
                     NPGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFDMTYIGPDGKEHRPVMLHR
                     TVLGSLERFLGILIEHYAGAFPFWLAPVQVRIIPVKEEHGDYAASIEDRIRMAGFRVE
                     KDTRDEKLGKKIRDAQLEKVPFMVVLGGREAENGTLSVRERSGGDLGSMSVEDFFSLL
                     EKQPDPASGTCNHGT"
     gene            17139..18611
                     /locus_tag="P5541_04310"
     CDS             17139..18611
                     /locus_tag="P5541_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRV71235.1"
                     /translation="MAEKKKADQWSSRWGLILSCIGMAVGTGNIWRFPRVAASQGGGA
                     FVIALLIGLFLWAIPLLMAEAVWGKVSRMGVIGSFKEMVGRKWTWMGTVVAVISLGIA
                     FYYAVVVGMCVRYFVYALTGVIKPGLDSEALWTAFYTTPGAMVPWHIITMVAATLILF
                     KGVSQGLEKINKILIPALFVMLVILAIRANMLPGSTEGLRFLFQPKWADLLKGRVWLE
                     AFTQVAWSTGAGWGLMLTYFVYTKKREDIALNATTVCFADTMAGFLAGLAVIPTVFAL
                     SPDPQAAVAAGNNGLAFIHLTNLFTVMPGGVLFASIFFLALIFAGFSSLLSMVELGIR
                     LLMDAGWERHKAAIAAGVGITVLGLPSSYYWWFQENQDWVWGVGLLLSGLIFSIASMK
                     IGIQKIWNEYIAPDSDLNAPWMWKLITLFPLWFVIIFGWWIKQAASWYPESYMKWLPI
                     SDYAFTVGTMVYQWAILVVLALATNNWLANKSTVKFEATD"
     gene            complement(18846..19286)
                     /gene="dut"
                     /locus_tag="P5541_04315"
     CDS             complement(18846..19286)
                     /gene="dut"
                     /locus_tag="P5541_04315"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRV71236.1"
                     /translation="MADTVTVRVFMEKSDLPLPFYATEFSSGVDLRSCESADIQPGQR
                     MAVGTGMKVEIPEGYECQVRPRSGLALKHGVTVLNAPGTIDADYRGEIRVILVNLGES
                     PFRIEKGDRIAQMVLTRVEKINWEESPELGDTERGSGGFGSTGK"
     gene            complement(19286..21580)
                     /locus_tag="P5541_04320"
     CDS             complement(19286..21580)
                     /locus_tag="P5541_04320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRV71237.1"
                     /translation="MSELFSIEVGGRTLNFETGKMAKQANGSVQVTQGETSVLVTACI
                     TDKPREGLDFLPLLVDYEERFYSAGKIPGGFIKREGRPSETATLTCRIIDRSIRSLFP
                     GHIRNDIHIVATTLSVDQVNPPNILAINGASAAIVLSGIEWEGPIAAVRIGQIDDELV
                     VNPTEDMMKDSALDLVVSGHAGGITMVEAGAKEVPETVLVDALELAHQEIKKIVAMFS
                     EMGRKAGKPAIHFPGPETFPEIDQWVETELSGDILEAVQIHDKKPRGDAVDAATARAI
                     ETLGEKFPGAGSYISAAVEKLLKKTMRKRVVEEGKRADGRAMDEIRPITCEVGVLPRV
                     HGSALFTRGETQALVTTTLGMVGEDDQLVDGLKHDEPSKRFMLHYNFPPFSVGEVRPM
                     RGPGRREIGHGALAERALRVLLPEQDEFPYAVRVVSDILESNGSSSMASVCGGSLSLM
                     DAGVPVKKPVAGVAMGLIKEGDDVAVLSDIQGLEDHYGDMDFKVTGTRDGITALQMDN
                     KAGGITREILQNALEQARRGRLHILEIMEGALAEPRKEVSSHAPRIYTLTVPMDKIRD
                     IIGPGGKTIRSIVAQTGVKVDVEDDGHVYVASPTSEAAEKALAIINDLVREVAAGEIF
                     FGKVTRLMAFGAFVEVLPGKEGLLHVSEISTTHIGKVDDVFSSGDTVLVVVKEIDDMG
                     RINLSRRRVLERKEEPEIAGKFGDAIILEDEREEKIKNLPPSAPSDRGDRNSRGSSQR
                     GGDRRPQSGGPRRSGSSDRNRDRS"
     gene            complement(21738..22004)
                     /gene="rpsO"
                     /locus_tag="P5541_04325"
     CDS             complement(21738..22004)
                     /gene="rpsO"
                     /locus_tag="P5541_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRV71238.1"
                     /translation="MLEKEKKQGIIEDYKVHSADTGSPEVQVAILTTRIRDLTEHLKI
                     HKKDHHSQRGLLKMVGRRRRLLQYLRNKDFNRYSELIQRLGLRH"
     gene            22222..22902
                     /locus_tag="P5541_04330"
     CDS             22222..22902
                     /locus_tag="P5541_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV71239.1"
                     /translation="MKILIIEDNRDLVQVLSEGFAENEFTVESAYDGIEGLNKIRNND
                     YDCIVLDIMLPEMDGYEVVEKAREEGKDIPVIMLTAKDTIEDRVEGLNKGADDYLVKP
                     FDFRELVARINAVTRRRSELKRPILHCGPLVVDPIARECRAEGEIVPLRRREFDILEL
                     LMRYENQVFTRERIITHVWQKEYDGTSNVVDVHVKYLRDKLRPYNLDTVVVTVRGVGY
                     KVTCPDYE"
     gene            22931..24421
                     /locus_tag="P5541_04335"
     CDS             22931..24421
                     /locus_tag="P5541_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV71240.1"
                     /translation="MDASVKKTADGRRPPWLRSFRYRLVFFSAFTITATIAVFASLVF
                     ILYTMKELQRVDDFLSRASAEAMVHFSEGGTYHDGSGKIAPGTVFFHVFSEDYSLVFS
                     APSGADASRIPTSLLPVPGPGGENLTFDPARSKVLPGSWWILPWNCLAERDLWRASVN
                     RAELSGKTVFLVTMISLEKMLESRHMLFWITVFSGMAAIISSILIGETVAGKAMKPLK
                     EINRALSRVSIENMHIEPPARETDREILEIVRHINRMLKGLDQSLRNLQQFTSDAGHE
                     LRTPLAIMRGAVDVALLKERESEYYIKKLHEVIYGIEDMQNLVGALLELARLDSLRGL
                     NKREPADLLIVAEDAINTVSPIIGKRGQRLKSELNPAPTTGREAMILRLINNLLENAS
                     KHSPPDSQIVIRTHADIERNGSILEIRDNGPGLSPEEINRCFDRFWRAESSRTTPGFG
                     LGLPLVQRIAQIHGAIIEIESEKGSGALFRVIFPLDKKALEEYDFE"
     gene            complement(24453..>25286)
                     /locus_tag="P5541_04340"
     CDS             complement(24453..>25286)
                     /locus_tag="P5541_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRV71241.1"
                     /translation="RSVRARDLNFDGFLQTDAAINPGNSGGPLLNLQGEVVGINTAII
                     PYAQGIGFAVPVNMAKQIMDDLVKYGKVNRGWLGIYLQPLSREFASAYGIETDSGAVV
                     SDVVKGSPAEKAGIARGDVIIEMNGRKILDHRDVIVGVRQQLAGQKIDVLILRRGSEK
                     KITVTLGNVPSVSVAGRSPSEPAPKVATRLGITVSPVTDETREEYGFSSDSGVVVTEV
                     QPGSVGNRLGLGRGDVILEINGQIVSDTAKWEEILSKGPRNVVFLVLREGRTFFVSAN
                     L"
BASE COUNT         5695 a         7291 c         6143 g         5957 t
ORIGIN      
        1 ggcgatatgt cgccagccat ggctcaaccg caaggattga aagccatgaa tcacctggaa
       61 taaagatacc ttatcccctc aaaaatttgc gggggagttc tggtcgatag ttttacagaa
      121 ttgccgccaa ggtcgaggct gtctatgttg agcgggaata caaaatcctc caggtcaaca
      181 acgggctgaa gatccctgac agcctgctcc actatcgatg tcttttcggc gttgttgacg
      241 aagagggaat aatccttgag ccagaccttt cttgatctga cctccagagc cgtggagagc
      301 tccacaagaa tcttcaggct gatgctcccc ctgacaacgt aataaccacg ggcaaaaatt
      361 cctcccggtc ttatctctac tcgtacattc tgccagttct cgtctatctg ttccctgaga
      421 gccgagttga tgtctccttc aagaacaatg gccgcaaaat taccggaaag tatctcttca
      481 acctttatgg attcagcatc cacccattcc gaaacggggt tgaattttcc gaaccttgtc
      541 tccaaagcca tctcttcgag tctgaaagcg ccaaaacggg cacctttgac gagcagatac
      601 atgtggcgca ggcttccatc ctccccaggc tcctcatcca gtaccatttc cattgaatcc
      661 ggatccagcc tctctgcaag ggcagcgaaa agtctctccc cggaagtagt ccattcgacc
      721 tctcccttga attccttcga cacctcggca tacacaggca tagcaatgaa aaggattgca
      781 agaaaaaccg ctgtcgcgaa ccttgatgat ctcaattcat gcacctccag accagtttgg
      841 ggatctattg ttttgtcctg tcaattgttt cggccatttc accaagggct gactccagtt
      901 tcttcgcgaa aagctccatt tgccctctga gggccgttct cgaagtgata ttgaagtgaa
      961 tgctgtcggc aaaagtgaga taacgaacac caagaccttc atcgtccacg gtcccgaacc
     1021 ccgcaaggta cattccgtcc actgctgaag tacttgtgga cagaagggtt cgggtaaggg
     1081 ttttaagccc ccggtcgctg aaaaattcag gttctccctg caagccgaga tcttttccca
     1141 tggtttcgaa gattcgcctg agaccgagaa ggtgtctttc gacccctttg ccggccttgg
     1201 attccttcat cctggagaca tgcttctcgg cggcagccct tatcgccacc gctttttcag
     1261 gatccgttgc tgatgggtcc ctcatgacct ttgtaaaaat taccgattcc tcagatacag
     1321 atcttacggc ctctgtcctg ccgtggagga actgccttgt cattacagcc tcgtaggtgc
     1381 tcctgggaac tccccagacg tcttgctgtg cgagctgcat cgccatctgt acaaaagcat
     1441 ctggagccac agaaagactc tttatccttt ctttcccaaa ggatccaaat atcaggacct
     1501 ccgtttcagt atcgcgtttc atcctgtcga aagccgtggc ggctgactcg atgatggacg
     1561 ccatgtcgtc atcgatatca aaaaccagat ctctccaggg acatttccca ttatttatcg
     1621 agccttcacc attccaggga tcgtcatttc ccacaaaaga cgcgaagcgg accattactg
     1681 atccatctgt ggcggtatgc tccatattaa tcgcgctggt tccgtcagga cagacgataa
     1741 actgcatacc cttgtcgaac catcttgagc gtccatcccc ggtgagcatg gtctccgaga
     1801 gctgttttat cccttcgggc tgcccttgct caaggcaaag aacaaacaat gccctttcga
     1861 tagtgtccag tatcttcctg ttgccggaaa tgatcgacag gttcgccctg gcctcggccc
     1921 agtcatccct gggaagggtc gtaagtacgc ctgcgggtga ggtggcctcg ccttccaggt
     1981 ccattatttc ctgaatggca ctctcgacag ccacgacatc aaaaaagttg ccctcatggt
     2041 ctatcacatc gacagaaaaa taccttccac ggctgatgac cacaatatgt ctctcgtaag
     2101 gttccgtggg atcatgggaa ataaccggat tgcgtatccg gtccatgcct ttttccggta
     2161 tcctggcgga ggagaacatt tttttgtact ggatcataca tatcggacgc cccttctcgg
     2221 tatccggcgg caactgccct gtatccacaa gttttttgaa tctcagtgca gagacgacca
     2281 gcccggccgc gtgtccagcc tgtgtgatct ccttcgggga tgccgatcgt ggagagaaca
     2341 tatagaaaat gttgctgtaa aagggcaaag atgccctgcc cgacaggtac atccactccc
     2401 agaagggttc aagccagttg gccacatcct ttctggcggc aaattctttc agtgcctttt
     2461 gaaggatcgg ccctggacca ctttcggaag caaactccct tgcggacctg cctgtccttt
     2521 ccagcgtatc gggatccaga aggggggagg cccactccag gagtcgttcg atggtctctt
     2581 ccaggtccgg caccggaagc gccggcagca acgcctggct ggaaaaagtt cccgctcgtc
     2641 ctttcggaga atcgcccatc aataattcct ccaatcctga aaaaccggtc actgctacat
     2701 ttgacccacg tagggggtcc tcctgatcgc cgaatgcaca tgattgatca tcgtgatgaa
     2761 gtcgaacaca ggtagatcaa cggcttcctg aactgccgcc gcatagggcg ggagatcgct
     2821 gcattccaga agcaccgccc cgacttccgg gttttccctt accatcctcc tggccacatc
     2881 ggtaacctct attcggataa ggtcggaatc gagagtcccc ttctcttcga gtatggacgt
     2941 tcggaactct tcctggtcct ccatcccgta aatggcaagg ggcatctcgg aggttatccc
     3001 taccgactcg aaatgctctt ctgtgaggca acttctgttg gccgttatta tcccgacctt
     3061 gcgccccgga gcaagggtcc tgaatatgaa aggaacctgc agcaggctgg agaggaacac
     3121 tggtattttt acggatctgg cgacctgcct ctggaaaagg gccatgaaac cgcatgcacc
     3181 ggtgacggcc cttacccctt ccgcctcgag atcccgggct gcctctacaa agggctcaag
     3241 aagagaagga tctcgctcgt ttagaagcct ctctattgag gctcccctta cttccctgta
     3301 ccgtacaggg aagttgtagg ttgtcgcatt tccgacattt cctggaacgc aggggtaaca
     3361 ggcatcgaga atgagaatgc ctattgattc accatcccat gaacgtgttc tgcttcttac
     3421 atgataaacg gtcattgtgc actctccctg tcattatgat tttttgcgat tattgagcct
     3481 ggccggtttc aggttttacg agaaccgtag tttccggcct gataacatgg ccgggcggga
     3541 aaccgggaaa ccaggaaaac cgttccactc tcctcggagt agctccgggg taaaccacca
     3601 ggacccagca gtcacctttt tcgtcaacca tgtgtgaagc tcccagcagc ggagggcctg
     3661 ggggattgac ccttcggaac caccctccga aatgattctc ccattcgccc catgaaaagg
     3721 aggggttctt ttcccatgct gattcttcct tgccttttga ggtaagcccc cttttctcgg
     3781 gggtatcctg ccaggggtac cagccgggag gttcgcacgg caccggaacg ccttccgtgc
     3841 catcctcgag gaaataccac tctcccttta catcgcagaa aaaccttggg ccagaacttt
     3901 cctgacctcc gacggcttcc ctcatccata cttggccggg aaggaaccag gcaccccata
     3961 aggggggatc cacattctgc tgatctggtc catcgggcag ggtcccgctt tcgagccatt
     4021 cgccgtaagc ctctacacag gctgctccag cgagcattac aaacaacaat aacgacagca
     4081 acatgaccct tttcatccat ttccacctcc attagttgcc tggtcacaag acacatccct
     4141 tacttgagat cagggactgg aactttgcct gttcaacacg tttcaaaatt tatcccttga
     4201 cgcgttcata atataccaga tactaaaagg gcccggaaaa aggtctcgaa attaaacccg
     4261 atcccggaaa cccttcagtg tttacgcacc catggggccg atgctcccgg aagacaaaaa
     4321 agaagggttc ccggccagga taaagccaga aacccttgct attactgatt tggcgtgccc
     4381 gtcgggattc gaacccgaga ccttcggctc cggaggccga cactctatcc agctgagcta
     4441 cgggcacaca tccgcggcgc taagggtaca gcgcgccccc cgtggttgtc aagtatatct
     4501 gttactcggt atatctgttg ctcggaaccc gtgaatcgaa aaagagtgaa aagaaagacg
     4561 caagtcgtga gacgaaagga gcaaaactca acggcaaaaa ggcaccccac aggcaatctg
     4621 aatgatctgg tcccgacgag gtcccgacaa attccagaga cgtatcaacg aggggattgg
     4681 ttcaatgagg agccttgtct tgggtgagat gtcctggttt gggtttttcc gtttcacctc
     4741 tcacgctgtc aggattctcc taccttctga aagaaactat ggtaactccg aagccgccct
     4801 ccccggcatt gccaagtctc tgatccacaa catagggcag atcctcgcag agagagtgaa
     4861 cagcccgtcg gagaacaccc tctcccctgc cgtgtatcac ggtgacttcc ccgtaaccgg
     4921 ccctgaatgc cctgtcaagg taaacttcca cctccggcat ggcctcgtcc agcgtcatcc
     4981 ccctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttttc ttcaattcgc
     5101 tggtttcctt tcttcccttc tcgaattcgc gccttgcagc ggattcggcc gctccctcca
     5161 ggtttttcag catttccctg gcggcctttt cggcatcttc caggatgccc ctcgccctgg
     5221 aatcagcggc cagaaggatc gattcttccc gttttgccat gctatcgacc tgctcgctat
     5281 acagtctttc catttccgcc agttttctgc gctcttcagc ggcttccctt gccgcatttt
     5341 caagttcaac ctgtttatcg aaaagctccg ccatgaggtt ttccgcggat ctgctctctc
     5401 cggaaaggtt ctgcatcgcc tggtctatta cgtcttcgga catcccgagc cttcgggcta
     5461 tcattatggc gttgctcttg ccgggaaccc ccatcagcag tctgtaggtc ggggaaaagg
     5521 tcacaggatc gaactccatg ctggccgatt cgaccccctt cgaagtgact gcatattttt
     5581 tgatagggtt gtgatgtgtc gtcgcaagaa caagacatct tttttccctt agtttttcga
     5641 gaatggccac accaagagcg gcaccctcct gcgggtctgt cccggccccg agttcgtcaa
     5701 gaaacaaaag gctgtctttt ccactctttt cgatcatcat aacgatgcgg ctgacgtgag
     5761 aactgaaagt tgagaggctc tgctctattc cctgttcgtc cccgatgtcc gcggccataa
     5821 aatccaccca tccagcgacg gaaccctccg cggccgggac aggaagaccc gagagagcga
     5881 gaaaaaccgc tatcgccacg gtcttcagaa caacggtctt ccccccggtg ttgggacccg
     5941 tgatcacaag ttgtcggaag cggtcaccgc agtgaatggt tatgggaaca caattcgagg
     6001 ctatcagggg gtttttcaac tcccggaaat aaaaacccgg tttttcccgt atctcgggca
     6061 gggaccaccc cgattcatca aggatgatcg aacatgcatg aagaatatca atgaatgcaa
     6121 ccgctttctg ggcatccaga atggcacttt tcttctccag gatcatcgcc gtaaggcctg
     6181 agaggatatt tctttcctct tccttttcct cgcccctgag gatctcggcc ctgttgttga
     6241 gtgtcacaac ttcccggggc tccatgtaga ccccttttcc tgagccggaa cgatccagga
     6301 ctatcccgtc aaaacggctc gcatcctccg cccttacaag caaagcaagc ctgcccttcc
     6361 tgaggtgaac caccctgtcc tgaaggtacg gtccaaattg ctgctgacct ccaatgtcca
     6421 tgcacctcga cctggcacga gaaagagtat ctctcagttc tccccgcact tttgaaagtt
     6481 tcggtgaggc ctgatcataa agttctccgt catcggcgat cacctggagg gcagaaagtt
     6541 ctttggacat gtcgcgaatc ccctccgcaa gaccctcaat acccggaatg tcacctttgg
     6601 cctcgtttgc agccaggcgg attttaattg ccagaccgat cagggtcctg aaacggagaa
     6661 gttcttctcc agtcaggtat gaactctgtt tcgcggtttc caggaatggc gacaccgggc
     6721 gaagagatcc gtcccagggg aaatctcctt ttttcgcaac atatctcata taggctctga
     6781 gaagtgactg tcggtggatt gcctgtttct gggtatggca cgggttaatg gacaaaaagg
     6841 cctgctggcc attttcggag cgggtttttc tggcaagcct ttcgagcacc gccggtatct
     6901 cgaccagttg gggggtcttt ggttctgttt tcaatttgtg ctgttaccgt ccttgccgtt
     6961 gccggtttcg gatggagcac cactccaggg aagagcttcg ggctccagca gacgcacccc
     7021 ggaaaggctt ttctggatca tgggccaact gcggtgggct aaagtcatag aataactctc
     7081 gttcatccag taaggtgaaa ttaccgggga gaagtacaac accccggcat agacgatcat
     7141 gagcaacgcg taggtcttga ccgcgccggc cacgataccc atccccctgt ccaccccgga
     7201 aaggttcgtg aatttaagaa atgccttgat aacccttccc gcaaaaactc ccaaaataag
     7261 gacagttacg tagaccgcaa ctatggaagc cgtctggaca gcagcagggc tcatcgggat
     7321 gaggggagaa accagttttg aaacctgtgg agacaccttc cacgccagga tgaacccccc
     7381 tatggtggtg accagtgata ttgcctcgcc cgaaaggccg cgcaagccgc ccctgataca
     7441 gaaggttaca aggatcagga gaaaaagcag atccaccccg cccgaaaggg tcacgaacca
     7501 tcactctccc ctgatatttt cctcagccgg ttctcggcat tttctattga ataggccagc
     7561 ttgaaacacg caagtagaag ccggtcctcc tggtcgatgg aaatatcgat cccggaaagg
     7621 ctgtcccttg ctatcctggc cacacgctcg aaacgctttt cgtcaagcag ggtcaatatg
     7681 gagtaacttt tcttgcccag ggtcacatac accctgcgcg gttcgtcagt caatcccggg
     7741 ccccctctcc gttcagaacc tgtccgtcag ttcgccttgc cggggcgaat cctcgctccc
     7801 gtcgtcttca tctggcaacc tttctccatc cgtccggagc gcctcctgga aagggtcgcc
     7861 accatccccg gaaaagccaa aagatgacct ggccttttca agaatagcct ccagccttct
     7921 ctccaacgct gttctttcct ccacgagcga ggcgagttcc ttttctcttt cttccagaac
     7981 ttccctgact acatccagtt cttcggccat ctggctcttt tcgtcccgga gggtctggat
     8041 ggtggatgaa accccgtcaa tcagcctttc aagattttcc atgtgttcga acatcataac
     8101 acctccagtc aaccattatc gaaggacaat acccttttct ttcagaagcg tccggagccg
     8161 ctcatgtatt aaatcgacct catcatctct gagagtcctg tcaggatccc tgtatgccag
     8221 cgaaaaggca aggctgcggt aaccgtcggg gatacccttc ccgctgtaga cgtcaaaaag
     8281 ccatgccttt tccagaaggg gatcggccag gttcctgagg agttcaagga cttcagccga
     8341 atactccccg tcttttacaa ggagtgaaat atccctgtat acgggaggat atcggcgggc
     8401 ttctccaaag ttggccaaac caccttcgat caggatatcc atgtctatct cgaaaaggta
     8461 gacaggaccg ggaatatcct gttcagcctc gatctccggc ttgagagaca gtatgtaacc
     8521 tgcatccttg ccatcagcaa ttacacgggc agtttttcct gcatgtccaa agggttcttc
     8581 tccccgcacc agttcgatcc agattcccct gccggcacag atagcctcca ggtctgacgc
     8641 aacagagtga aaatcctcga cagcctgcgc tccccagggt gacctggtgt ctttcccggg
     8701 gcagacgcac ccgcacagtt tcagatgctc ctcgacctct tccccttcgg gaaaaaacac
     8761 ggccccgacc tcgaacattc ttacaggacc tctccaaccg gaccttatat tctccttcag
     8821 ggccttgaca agtccgggag caagggttgt cctcaggacg gaattatcca acgtcagcgg
     8881 attagagaga gcaacggccc tggacctcct gtcctccggg gggatcctga atttttcacc
     8941 gaagtccgga gagataaaac tgtaggttat gacctcggca tacccccggc ccatcgccgt
     9001 tcctctgatg cttctttcgg ccctcatcct gtccgtgata tctccggtag tgtgcatgac
     9061 gggaggaagg gccggggcga tcccgtcata gccccttatc cttgcgacct cctctatcag
     9121 gtcctcctcg atggtaatat caggcctcca ggatgggacg atatagctcc tttgcgagga
     9181 agaaccgcct tcatcgacaa aaccaagtcg atcaagaata gcggtcgcca tttcaaggtc
     9241 atcgctgttg attatcttgt ccagcaaggc cgaagagagg cttgcatttc ttccagaacc
     9301 cgaaggtttt cctgaagata tccacccgcc cggagtacct cctccccatt cggagatcat
     9361 atccatcacc atggccagag ccggcttgac cttgaatggg tccacgtagc gagagaatct
     9421 gtacgccgcc tcgctgggaa gccccagtct tctcgaggta cggcttaccc ttatgctgtc
     9481 aaagtgcgcg gattcgagaa gaaccctccc tgtttccggg ccgatctcgc tttcgcttcc
     9541 tcccatgacc ccggcaagag ctacagcctt tccaccgctg gtaataagaa gatctgaacc
     9601 atcgaggacc cttgccttgc catcaagggt ctggagtatc tccccgctgg atgccgatct
     9661 tacagtgatc tcaccacccg gaagaccgga agcatcaaaa gcgtgaaggg gctgccctgt
     9721 cagcagcatg actatgttgg tcgcgtcaac aacgttggaa accggtctca tcccggacag
     9781 aagaagtctt attctggttg atacagggga gggggctatg cgaatgtcgt caatggaccc
     9841 aagagcatag aaaggacaac ccggatccag gagcgtaata cccgaaaaac cctccagtga
     9901 ttctgactct tcagggataa cagggtcctt gacccggaac acggcaccgg gaacgaccgc
     9961 aaagacttcc cttgccaccc ccatcatgct caggagatcc cccctgttgg gagtgatgga
    10021 taactcaaga accgtatcat caagtccaag ggcttctctt gcatccattc ccggggaata
    10081 accatctccc agcctgagta tcccgaactc gtcggagatc tccggcatgc cgatctcttc
    10141 tgcggaaagg accattccct cgctgataac tccttcaaaa tcccttgttc ctatcacgtc
    10201 accgcttgcc agcacacacc ctggaggacc ccacaggact gtatccccct catgcagatt
    10261 ccttgcggct gtcacgcaga cacccttact ttcccctgcc tcgacctcga ggagaaaaag
    10321 ttccggtcgg gaagggtgaa ccgatatccg ggagaccctg gaggtcagcg ctccacgaaa
    10381 gagcggaacg ggcgtcgata ttgattcaac ctcagacccc gtatcggtaa gacgcctcgc
    10441 gagggcctcc gtgtccaggg ggatatcgac cagttctctg agccagttca aggatacaag
    10501 cattatctcc tgccccctgt cgatgtgagg aaagaaacat ctccctcgaa gaacactcta
    10561 aggtccttca gcccatattt aagcatggct atcctgtcca gacccatgcc ccaggcaaag
    10621 ccgttatagc gcaccggatc tatgcccccc gccctcagta cgttggggtg ggtcatgcca
    10681 aggccggaga tctccagcca gccggtaccc ttgcaaatac ggcaggatgg gtccttgccg
    10741 gagcaggcga cgcattcgac gtccatctcg agcgatggct cagtgaaagg gaaataactg
    10801 gcacggtagc gcgcggcgag gggacgacca aatattctgt tgacaaaggc ttcgagacag
    10861 cccttcaggt gggcgacgga aatattttca tcgataagaa gaccctccag ctggtggaac
    10921 atcggagaat gggtctggtc gctgtccctg cgaaaaacct tgccaggtat tattattctg
    10981 agaggagccc cccatttgcg catggaccga acttccacgg gagacgtatg ggtcctgagc
    11041 aacaatccgt tctcgaggta gaaggtgtcc tgcatatctc tcgccggatg gtggggaggt
    11101 atgttcagcc cctcaaaatt gtgaaagtca tcttcaactt ccgggccgta tgccaccgag
    11161 aaccccatgg aggcaaaaat gtctatggct tcctgggtca cctgggcaac ggggtgaaag
    11221 gcaccccatg gacgccccct acccggaaga gtaacgtcta tccactcctt cgtctccctg
    11281 gcggccaaat cctgaccagc acgttctgtt tcccgggcgg acaggtcttt ttccatctcg
    11341 tccctgagtt cgttaaggac tttcccggcc tcgggacgtt cctccgccgg gagagagcca
    11401 agccgcttga gcagcgaagt aagttcaccc ttcttcccca gaaaacgcac tcttagagac
    11461 tgtatctctt cccgggtgaa ggctttggag agttcctcgc gaaataataa acgtatcctg
    11521 tcgagatcgt cagatatttt gttcaagagt ttcccctccc tgcacttttt ccttgcatct
    11581 gtctatccag catccgatat cttcctgttt tccagtgacc atcagaaggt cattatcttc
    11641 tagcacagtg tcagcccttg gaagaaagcg gtctccgttc ctgttcacca ggagaacaac
    11701 ggcattgtac tgatgacgaa aatcgagttc ctgaagagtc ttgccgaaca tctccggcag
    11761 tgcaccaact tccccaacta tcaggccact gcccggtatc tcggaaaaac gggaaaggtg
    11821 gggatggact atctgctccg cggtccgaag accgagatct ctctctggaa aaacgacccg
    11881 ctgcaccccg acccgcgcaa gaacccttgc gtgaagggcg ttcctggcac gtgcaacaac
    11941 gagaggaacc ttgaaaccga caaggatagc agaagcaagg acgcttgcct caaggctttc
    12001 gcctatggcc acaacagcca catcgcaatc ctttacaccg accttctcaa gggccgcctc
    12061 atcggttaca tccagctgtg cagtcagatc tatagtttca gagagggcct ccaccctgga
    12121 gcggttcctg tccaccccta tgacgctctg gccaagttcg gccagacgca gacatatcgc
    12181 ctcaccgaag cgtccaagac ccagtacgat gcaggttctt gttttctccg acatcctcat
    12241 tccccccctt tagccaacgg gtatgttggt atccgtatat gataccctcg cagtcggcgc
    12301 atggtctaca agagcataca tgaatgttat tagcccgaca cggccccaga acatcaaaat
    12361 taccagaaca agtttacccg gagccgtgag atggggagtt ataccggtag acaaccccac
    12421 ggtcccaagg gctgatatca cctcgaaaag aacatccctt agcccgaagg gttcaaatac
    12481 cagcaacaac cccgtcccgg caattatcgt tccaagatat accacaagga gtgacagcgc
    12541 cctttttatg attcttgacg gaatgcgcct gttccagagg gttacttccc ccccacccct
    12601 cagttcgctt gaagtcacag ccagaagaac ccccagggat gtcgtcttta ttccacctcc
    12661 tgtagaaccc ggtgaagcac cgataatcat caggatgcat gtcagaagaa tcccggcaga
    12721 tgacagcgat gaagtgtcca gggtgttgaa accggctgtc ctcggtgtga tcgagtggaa
    12781 aagagagttc catatcctga aagcaaaggt aagcccttcc agggtccccg tccattccga
    12841 aaccgcaaaa agggccgccc cgatcgcgac aagccataga gaaaccgaaa gcacaagccg
    12901 ggtatgaaca gaaaggggac gaatccggcc tcccctgaaa ctggaccaga tctccagcag
    12961 agggataaaa ccgagtcccc cgaaaaaaac aagggacatg accgcacccg gaaccatcca
    13021 ggtcgcctgg tagcccatca ggctatccga tatcggagaa aagcccgcat tgcaaaaggc
    13081 acttatactg tggaacacag ccatgtaggc tgcacgggaa ataccatggg taccaagaaa
    13141 acccgcaaaa agcaccaatg cgccagccat ctctattacg attgtgaaac ggaccaccca
    13201 gaggatcagc cgaaccgccc cctggggagt atccagaccg aaacctccct tgatgagaag
    13261 cctctgcctt atcccaatcc gccttcccca gaccagagag agcgcggtcg ctgccgtcat
    13321 cacccccagg ccccccagct ggataagaaa aaggaggata tactgcgaaa tcagggagag
    13381 gtccgaaccc gtatcgacca cgacgagccc tgtaacgcaa acagcggaag tagccgtgaa
    13441 aagggagtcg acccatccca gcggatcaaa aaaatgactg gatatccata aagcaagggt
    13501 tcccgccagg atcaatgaca ggaacccgaa aatgatagtc cgttcaatac cgaacgaacg
    13561 gaacgttctc aaggctatct cgcaagcgcc tccctggcct ggtcaaccag cctggcgaag
    13621 gcgggcatat cctggatcgc cagatccgcc agaaccttgc ggttgatctc gatgttggcg
    13681 cacttgagac cgttcatgaa agagctgtag gtcatctggt tcatgcgcgc ccctgcgctg
    13741 atcctggtaa tccagagttt tctgaaattc ctttttttgg cccttctgtc cctgtacgca
    13801 ttggccatgg agtgcaggta cgcttcctgg gccctnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnncctct tccgctcttc ttgaatacaa actttcctgt tcccgtcgtg
    13981 gaaaatcttt tctttgttcc agagtgtgtc ttcattttgg gcatttcctg ttttcctcct
    14041 cttttcagga actgcggttt ttaattatcc tcagtggcag atgtttcccc tgcgctgctg
    14101 acctgttccg tggcctcatc tgcgggctgc ctgctttttt tctgagtctg gggcaccggg
    14161 gtcagcatga gcctcatgta tctgccttcc atccggggag gcatatcgat cttgcctatt
    14221 ccttcgcaat ccctgacgac cttgtccaga acttcccggc ccctgtcgag aaaggccatc
    14281 tcgcgccccc tgaaaaagac agcgaccttt accctgaagc cggaaaggag aaaatccttg
    14341 accgcgcgca ccttgaaatt gtaatcatgg agatcgatct taggtcgcat cttcatctcc
    14401 ttgaccacct gtcccttggt cttcttcctg gcatccttgt ctctcttctg ctgctggaac
    14461 ttgaacttgc cgtaatccag tatcctgcag accggaggat cggcctggcc cgaaacctcg
    14521 acaagatcca gctcacgctc ttcagcgagt gccaatgccc gggacgttgg aacaactccg
    14581 accttgaccc cctggtcatc gatcaggaga acttcctgaa cccgaatttc ttcgttgatg
    14641 cgcggttcat cgcttttttt agctataatt cgtcacctcc atcagataat taaatgggac
    14701 cggacattca agcccggccc caatatcggc acaaaatcag acaacgcaac tcgctgtccg
    14761 gtcgacatac cctgcaacaa ctcaccaagg agcgtcaaag ggtgggaggc atggctcctc
    14821 ttgcatattc ttttgtctaa acaacaatac ccgatccaag gatggatact agcacattat
    14881 gtcccgtgat tgcaagttcc ggaagccggg tccggctgtt tttccagaag ggagaagaaa
    14941 tcttccacac tcatggaccc aaggtctccc cccgaacgtt ctctcaccga aagtgtcccg
    15001 ttttcggcct ctcttccccc aagcacaacc atgaaaggaa ccttttccaa ctgggcatcg
    15061 cggatctttt tgcccagttt ttcgtccctg gtgtcctttt cgaccctgaa accagccatc
    15121 ctgattcgat cctcaattga cgcggcataa tctccatgtt cttccttgac gggaataatt
    15181 ctgacctgta cgggggcaag ccagaagggg aaagcacccg cataatgctc tatcagtatc
    15241 ccgaggaacc tttcaaggct gcccaggacc gttcggtgaa gcataacggg cctgtgctcc
    15301 tttccgtcag gaccgatgta ggtcatgtca aatttctcgg gcatctggaa atcaagctgg
    15361 attgtcccgc actgccaggt cctgccgatg cagtcctcga ggtggaagtc gatcttgggt
    15421 ccgtagaagg caccatctcc agggttgatc ctgtactcga taccgaactc ctccagggcc
    15481 tccttcaggg ctttttccgc agtgtcccac atcgcgggat cgcccattgc cttctccggt
    15541 ctcgtggaaa gttccaccct gtatgggaaa ccgaagacat ccctgtatat gaatctgacc
    15601 agatccatta ttccgatgat ctcctgtttg atctgctccg gtgttacgta gagatgagcg
    15661 tcatcctggg taaagcacct gaccctcatc aatccgtgca gaactccgga gcgctcgtgc
    15721 cggtgaaccg tcccgagttc ggctattcgc agagggaaat cacggtaact ccggattttg
    15781 gtcttgtaga caagaatacc cccgggacag ttcatcggtt tgacagcaaa ggggcgctcg
    15841 tcgatctccg tgaaatacat gttctcccgg tagtggtccc agtgccccga ccttatccag
    15901 agttcctggt caagaatgag gggagtcctg atctccgaat aacccctgcg aatatgctcc
    15961 gcccgccaga agtcgaccag tttgtttatt acgaccattc ccctggggtg gaaaaagggg
    16021 aagcccggcc cctcgttatg gatactgaaa agatccagat ccctgccgat cttgcggtgg
    16081 tcccttcgcc tggcttcctc gaggcggttg acataccttt ccagggcctc ggccgaatcg
    16141 aaggccgttc cgtatatcct tgtgagcatt atgttcttct cgtcaccccg ccagtaggca
    16201 ccggctatag acaaaagtct gaaatgcttc aggtccgatg tggatgacac gtgaggtccg
    16261 cgacagatat cggcgaactc cccctggcga tagatggata tttcatcatc ctcgatccct
    16321 tcgagcagtt ccagtttata gaccgcatcc ctgtcacgaa aaaaattctc cgcctctttc
    16381 ctgggaagga aaactctctc cactggaagg gactctttcg agatacgatt catctcctcc
    16441 tcgatcctgg gaaggtcctc ttccgatatg gttcccttta tctccatatc ataatagaag
    16501 ccatcctgta tcgacggccc tataccgagc ctggttcccg gaaagagcct ttgtacggcc
    16561 tgggccatca ggtgcgatac ggaatgcctg atcatgtcca gaccttcagg gcttccagcc
    16621 ctgacgggct caattgtcga attacctttg acctctgtcg aaagatccgc tacgacacca
    16681 ttgaccattg ccgcaacgga atcgcccgat attccccatt ttttcagaat ttcgccggct
    16741 ttcaggatct tctcactttc aagaatcctg tcttcgggac ccctgtattc tggcaataga
    16801 ttcatcctcc ctgatagata ttcgggtttt ttaaagcctg acaaagctgg ttgtccggtt
    16861 tcgtggaaag aattgtatca cagccacgcc tgttgtgaac tttaaacaac tgcagatgta
    16921 tataatatac agcctccatg tagtgttgca acagcatata atgtgttcag ttctttcatt
    16981 atttgttttc tgcagttcat tttttcagga agggagtggt tcgcggaaga tagttaaaaa
    17041 tttctccagc accaggcatc aggtttttcc gaaactgttt tttttcgttt tacaaagtca
    17101 gggatccaag tcaggttttc taaggagggg tttgcagaat ggctgagaaa aaaaaggctg
    17161 accagtggtc atcgcggtgg ggcctcatcc tgtcctgcat cggtatggca gtcgggacgg
    17221 gcaacatctg gaggtttccc cgtgttgccg cctcccaggg cggtggtgcc tttgttattg
    17281 cgctcttgat tggacttttc ctttgggcca tcccgctcct catggctgag gccgtatggg
    17341 gcaaggtatc aaggatgggc gttatcggat cgttcaagga aatggtcggc aggaagtgga
    17401 cgtggatggg aacggtagtt gcggtgatct ctcttggcat cgcgttctac tacgccgtcg
    17461 tcgtagggat gtgtgtacga tatttcgtct atgcccttac cggggttatc aaaccgggtc
    17521 tcgactccga agctctctgg actgctttct acaccactcc cggagcgatg gtcccgtggc
    17581 atataatcac catggtagcc gcaacactga tcctcttcaa gggcgtatcc cagggacttg
    17641 agaagatcaa caaaatcctc atccccgctc tttttgtcat gctcgtgatc ctcgcgatca
    17701 gggcgaacat gctcccgggg tcgacggagg gtctccgttt tctcttccag cccaagtggg
    17761 ccgacctgct caagggccgt gtctggcttg aggcattcac acaggtcgca tggtccactg
    17821 gagccgggtg gggcctcatg ctgacatact tcgtctatac gaagaagcgc gaggatattg
    17881 cccttaacgc cacaactgtc tgcttcgcgg acaccatggc aggcttcctg gctggactgg
    17941 ccgtcattcc gacggtcttc gctctttcac cagacccaca ggcggcagtc gcggctggaa
    18001 acaacggcct cgccttcatc cacctgacga atctcttcac cgtcatgccc ggaggcgtcc
    18061 tcttcgcttc aattttcttc ctggccctta tctttgcggg cttcagttcg ctcctctcga
    18121 tggtcgaact gggcatccgc ctgcttatgg atgccggatg ggagcgccat aaggcagcca
    18181 tagcggccgg cgtcggcatc accgtccttg gactgccctc atcctactac tggtggttcc
    18241 aggagaacca ggactgggtt tggggcgtgg gacttctcct cagcgggctg atcttctcca
    18301 tcgcatccat gaagatcggc atccagaaga tctggaacga gtacatcgct cccgattcag
    18361 acctcaacgc gccctggatg tggaaactta tcaccctctt cccgctctgg ttcgtgatca
    18421 tcttcggatg gtggatcaag caggccgcaa gctggtaccc ggaatcctac atgaagtggc
    18481 tcccgatctc ggattacgct ttcaccgtag gtaccatggt ctaccagtgg gcaattctgg
    18541 tggtgctcgc cctggccacc aacaactggc tggcaaacaa gtccaccgtc aagttcgagg
    18601 caaccgacta gggaggtttc ccttttccca aatttaagga aatctaccga cagggaggga
    18661 tacatatggg ctggcagttc aatatggtaa taattatggc tcttgtcttc ggtggcacag
    18721 gctggtgcgt ttaccggacc atgaaatccg aggataagaa atagcacagc gggtaaaaat
    18781 acagggggcc gtctcttgaa gaggcggccc ccctttttat gtcttcttgt taccgatatt
    18841 ctgttttact tgccggtact tccgaaacca ccgcttccgc gttcggtatc acccagttct
    18901 ggtgattcct cccagttgat cttctcaaca cgggtcaaaa ccatctgggc tatcctgtca
    18961 cccttttcga tcctgaaggg cgattcacca aggttgacca ggattaccct tatctcgcca
    19021 cggtaatcgg catctatcgt tcccggagcg ttcaatacag ttaccccatg cttcagggca
    19081 agaccgctcc tgggtctgac ctggcactca tacccctccg ggatctcgac cttcattccg
    19141 gtgcctacag ccatccgttg ccccggctgg atatctgcgc tctcacagga acgcaggtca
    19201 actccggatg aaaattccgt tgcgtagaag ggaaggggaa gatcgctctt ctccataaag
    19261 acccttacgg taacggtatc tgccatcagg atctgtccct gttcctgtcg gaggaaccac
    19321 tccttcgggg gcctccgctc tggggccttc tgtctccgcc gcgctggctc gaccccctgg
    19381 agtttctgtc tcccctgtcg gaaggagccg aagggggaag attctttatc ttctcttccc
    19441 gctcatcctc aaggatgatc gcgtcaccaa actttccggc tatctccggt tcttctttcc
    19501 tttccagcac acgccttctg gagaggttga tccttcccat gtcatcgatc tccttgacaa
    19561 cgaccagcac agtgtcgccg gaactgaaca catcatcgac cttgccgata tgggtggtac
    19621 ttatctcgct gacatgaagg agtccctcct tgccgggaag aacttccacg aaggccccga
    19681 aggccataag tcgcgtgacc tttccgaaga atatctcgcc tgccgcgacc tcacgtacaa
    19741 ggtcgttgat tattgcaaga gccttctcgg ccgcttcgct ggtgggtgag gcaacgtaga
    19801 catgaccgtc atcctcgaca tctaccttta cgccggtctg ggcgacgatg ctgcggatag
    19861 tcttgcctcc aggtcctatt atgtcgcgga tcttgtccat gggtaccgta agtgtatata
    19921 ttctgggagc gtgagaggat acttcctttc gaggttcggc aagggcacct tccattattt
    19981 caagaatgtg gagtctcccc ctgcgggcct gttccagagc gttctgcagt atctccctgg
    20041 tgataccgcc cgccttgttg tccatctgga gcgccgttat gccatccctt gtgccagtta
    20101 ccttgaaatc catatcgccg taatgatctt cgagtccctg gatatcagaa aggacagcta
    20161 cgtcgtctcc ttccttgata agccccatcg cgacgcccgc gacgggtttt ttgacaggga
    20221 caccggcatc catgagagaa aggctcccac cgcagaccga tgccatggaa ctggatccgt
    20281 tcgattccag aatatccgag accaccctca cggcgtaggg aaattcatcc tgttcgggca
    20341 aaagcaccct cagggctctc tcagcaagtg ccccgtgccc gatttccctg cgtcccggac
    20401 ctctcatggg acgaacctct cccaccgaga agggcgggaa attgtaatgg agcataaaac
    20461 gcttggaggg ctcgtcgtgt ttgagcccgt ccaccaactg gtcatcttct ccgaccatac
    20521 caagggtcgt tgtcacgaga gcctgcgtct cacccctggt aaacaaagcc gaaccgtgga
    20581 ctcttgggag cacacccacc tcgcaggtga tgggacgaat ctcgtccatg gctctcccgt
    20641 cggctctctt gccctcttca accactcgct tcctcatggt ttttttgaga agtttttcca
    20701 cggcagcgga aatataggac ccggcacccg ggaatttctc ccccagggtc tctatggcac
    20761 gagccgttgc ggcatccacg gcatctcctc tgggtttctt gtcgtgtatc tgtactgcct
    20821 cgagaatatc gccagagagt tcggtctcga cccactggtc gatctccggg aaggtttcag
    20881 gtccaggaaa atgaatagca ggtttaccgg cttttctccc catctcggag aacatcgcaa
    20941 caatcttttt tatctcctgg tgagccagtt cgagggcatc gacaagaaca gtttccggga
    21001 cctccttcgc ccctgcctct accatggtaa tacccccggc atgcccgctg acgacaaggt
    21061 ccagagcgct atccttcatc atgtcctcgg tggggtttac caccagttcg tcatcaatct
    21121 gccctatcct gacggcggca atggggcctt cccattcaat acccgaaaga acaatggcag
    21181 ctgaagctcc attgatagcc aggatgttag gcgggttgac ctgatcgacc gaaagtgttg
    21241 tggccactat atggatgtcg ttgcggatat ggcccggaaa aagagaccgg atagacctgt
    21301 caataatcct gcaagtcagg gtggccgttt ccgagggcct gccctctctc ttgataaaac
    21361 ctcccggtat ctttcccgca gagtagaacc tttcctcgta atcgacgaga aggggcagaa
    21421 aatcaagtcc ctctctaggt ttgtcggtta tacacgcggt caccagaacc gacgtctctc
    21481 cctgtgttac ctggacggat ccgttggcct gtttagccat tttacccgtc tcgaagttca
    21541 gcgttcttcc acctacttcg atcgaaaata attcagacat tattgtttcc tcctcctgat
    21601 ttttccttcg ttttatccgt tacaggataa cacaaggaaa gccctgccgg gcatacacaa
    21661 aaaaaccggg agcctgcatt tccaggcccc cggcaggtcc cttgcaatat tacaggccgg
    21721 acaagcaaac cagcccgtca gtgcctgagc cccagcctct ggatcagttc actgtagcgg
    21781 ttgaaatcct tgttcctgag atactgcaaa agcctcctgc ggcgacccac catcttgaga
    21841 agaccccgct gggagtggtg atcctttttg tggatcttca gatgctccgt aaggtccctg
    21901 attctggtgg tgagaatcgc cacctggacc tcgggggatc cggtgtcggc cgagtgtacc
    21961 ttgtaatcct cgataatacc ctgtttcttt tccttctcca gcaacttgct tcacctctga
    22021 aaacttattc ccgaaaccaa gagatgcgac catgcgcgat acctagtaac gggttttgcg
    22081 attataccac cagcacaaga agggggcaaa caatcggccg ggtaagtttc tgctcattct
    22141 gcactcgaca ttttcttaat tatgtggttt tattattaat gaaaaaatat ttatcaatca
    22201 tcgaaaggaa ggagagatac catgaagatc ctcataatcg aggataacag ggatcttgtc
    22261 caggttctct cggagggatt cgccgaaaac gaattcaccg tcgaaagcgc atatgacggt
    22321 atcgagggac tcaacaagat ccgcaataac gactatgact gcatcgttct cgacattatg
    22381 ctccccgaga tggacggata cgaggtcgta gaaaaggcac gggaagaagg caaggacatt
    22441 ccggtaataa tgctgaccgc gaaggacacg atcgaggaca gggtcgaagg actcaacaag
    22501 ggtgccgatg actacctggt caaacctttc gatttcaggg aactggtcgc gaggatcaac
    22561 gctgttacga gaagacgatc ggaactcaaa agaccgatcc ttcattgcgg acctcttgtc
    22621 gtggacccta tagccaggga atgcagggcc gaaggcgaaa ttgtgccgct tagaagacgg
    22681 gaattcgaca ttctggaact tctcatgcgg tacgagaacc aggtctttac acgggagaga
    22741 ataataactc atgtatggca gaaggaatat gacggcacga gcaatgtggt tgatgttcac
    22801 gtcaagtacc tcagggacaa acttcgcccc tacaatcttg acacggtcgt agttaccgta
    22861 agaggtgtcg gttacaaggt cacctgcccg gattacgaat aaaccccttt tccgggggac
    22921 gggggtctcc atggatgcat cggtgaaaaa aactgcagac gggcggcggc cgccatggct
    22981 ccgctcgttt cgttacagac tggtattctt ctcggcattt acgataacgg ctaccattgc
    23041 cgttttcgcg tcccttgttt tcatactcta cacgatgaag gaactccagc gtgtcgatga
    23101 ttttctctca cgcgcctcag ccgaggctat ggtccatttc agtgagggtg ggacatacca
    23161 tgacggttcc gggaagatcg cgccgggaac cgtttttttc catgtcttca gcgaggacta
    23221 ttcactcgtt ttttccgctc cgtcaggtgc cgacgcttcc cggatcccga ccagcctgct
    23281 gcctgttcca ggccccgggg gtgaaaacct gacctttgac cccgcaagga gcaaggtcct
    23341 tcccggatcc tggtggatac tgccgtggaa ttgcctggcc gagagagatc tgtggagggc
    23401 ttcggtaaat cgggcagaac tttccggaaa gaccgttttc ctggtcacca tgatatccct
    23461 ggaaaaaatg ctggagtcca ggcacatgct cttctggatt actgtttttt caggtatggc
    23521 ggcgatcata tcctcgatcc ttatcggaga aaccgttgca ggaaaggcca tgaagccgct
    23581 gaaggagatc aatcgcgctc tttcgcgggt gtcgatagag aacatgcata tagaaccacc
    23641 cgccagggaa accgacaggg aaatactgga aatagtccgc cacatcaaca ggatgctcaa
    23701 aggtcttgac caaagcctca ggaatcttca acagttcact tccgacgcgg ggcacgaact
    23761 caggaccccc ctcgctatca tgagaggcgc cgtcgacgtg gcactgctca aggaaaggga
    23821 atcggaatat tacataaaaa aactccatga ggtcatatac gggatagagg acatgcaaaa
    23881 tctcgtggga gccctgctgg aactggcaag gctcgactcg cttcggggcc tgaacaaaag
    23941 agagcccgcg gatcttctta tcgtagccga ggatgccata aatactgtgt ctccaataat
    24001 cgggaagcgg ggacaaagac tgaaatccga gttgaatccg gccccaacca ccggcaggga
    24061 agcaatgatc ctgaggctga taaacaacct cctggagaac gcttccaaac attcacctcc
    24121 tgacagccag atagtaatca ggacccatgc ggacatcgag aggaacggat cgatcctgga
    24181 gatcagggac aacggacccg ggctgagccc cgaggagatc aaccgctgtt tcgaccgttt
    24241 ctggagagcg gaatcatcgc ggaccacgcc gggtttcggc ctggggcttc cgttggtcca
    24301 gaggatagcg cagatacacg gggcgataat agagatagaa tccgaaaagg gctccggagc
    24361 tcttttcagg gtcatcttcc ctctcgacaa aaaggctctt gaagaatacg acttcgaata
    24421 gcaggagggg gcctgacggc cccctcctgt tcttaaagat tggctgaaac gaaaaaggtc
    24481 cgaccttccc tcaggaccag gaacaccacg ttcctcggcc ctttcgagag aatttcttcc
    24541 cattttgcgg tatcgctgac tatttgcccg ttgatctcca gaattacatc gcctctgccg
    24601 agaccaagcc tgtttccgac ggaccccggc tgaacttcgg ttaccaccac cccggaatca
    24661 gaggaaaaac cgtactcctc cctggtctcg tccgtcacgg gcgaaacggt gatcccaagc
    24721 ctggtggcaa ccttcggggc cggttccgaa ggagatctcc ctgcaacgga aaccgaggga
    24781 acattcccga gagtaacggt aatcttcttt tctgaacccc ttcggaggat caggacatct
    24841 atcttctgcc ccgccagttg ctgtctgacc cccactatta catccctgtg gtccaggatt
    24901 tttctgccgt tcatctctat gatcacgtcg cccctggcga tacctgcctt ttcggccgga
    24961 gatcccttga ccacgtcact gacaaccgca ccggaatcgg tctctatacc gtaggcgctt
    25021 gcaaactctc tcgaaagagg ctggaggtaa attcccagcc atcccctgtt aaccttaccg
    25081 tatttgacaa gatcgtccat tatctgtttc gccatattca cgggtaccgc aaatccgatc
    25141 ccctgggcat aggggattat cgcagtgttg atcccgacca cttccccttg aaggtttagc
    25201 agtggcccac cgctgttgcc cgggtttatc gccgcatcgg tctggaggaa accgtcaaaa
    25261 ttcagatccc tcgcccttac ggaacg
//
LOCUS       DAOTIQ010000021        24485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2398_length_24485_cov_8.090790, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000021 DAOTIQ010000000
VERSION     DAOTIQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 24485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24485
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2398_length_24485_cov_8.090790"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="P5541_04345"
     CDS             complement(<1..736)
                     /locus_tag="P5541_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV71242.1"
                     /translation="MMVTSLLAFVLVIAICVLSHEAGHFFIARRCGVKVHEFSFGMGP
                     SLASFKRKDTLWSLRAVPVGGFVRLAGMGEEQTDEVIPPGGSFQEKSAWKRLAILAGG
                     SASNIFLAVLITAILLWGHGVLDLSSTRIGEIMAGYPADKAGLVAGDRIEAVSGSIVS
                     DWTELSSAIRERSVEGPVLILFERDGEIKEIFLEIPFDPINQTYLLGIRPSIKKYPLG
                     RAFTGSIGYIWNFSLDIVRGILQWVTG"
     gene            complement(733..1923)
                     /locus_tag="P5541_04350"
     CDS             complement(733..1923)
                     /locus_tag="P5541_04350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRV71243.1"
                     /translation="MMIGTKRNIAVIGATGSVGGAVLEACLMHYDIFNVWALAAAKNV
                     EGLLSLGKKFESDLLVLSDEMAAMELQNKGSVSFECRGGAGAIESIIEDPRCEEVVFA
                     SSGTDALPALVMALKAGKKIFLANKEIVVAAGEWVMPYARAGALIPLDSEHNAVWQSL
                     KGEDRENVRKIFLTASGGPFLELPDEELSVVTFEMAIRHPVWPMGSKISVDSATLMNK
                     GIEIIEARQLFSLPPEKIDAVIHPGSQVHALVEFIDGSVKMLLSRPDMRLAALAALGY
                     SGRLQNTDVNLNPPEPKELLLEFRQPDENKFPCLKIAKEVCRLGGAYPPLLIGADEAA
                     VDLFMKGRIGFTDIPLFIQEALESFSGEAPGSWEEALYLVEMGRKSVLEKISSDMRGM
                     GREK"
     gene            complement(1923..2741)
                     /locus_tag="P5541_04355"
     CDS             complement(1923..2741)
                     /locus_tag="P5541_04355"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRV71244.1"
                     /translation="MALSPDSSNGFITRSVSGAVICLAVLGGIYLGNPWWYAICLCIS
                     ILSLSEFYRITGFRFHVSRITGYPAAFFLVTAAAFSSDSSVILPILAVVPLAVLFIEI
                     ARRHLTGSSHGIASSCATPAGLLYIVLPWSFLVILRDHIWGMYLLTALFACTWSCDVA
                     AYLVGSHWGKTRLCPSVSPKKSIEGFAGGLAGSILCGGGLSFLWGVAPLPLVLIALIC
                     GTFGQAGDLAESLIKREAGVKDSGDLIPGHGGFLDRFDSILVSSVLVFAFFGVL"
     gene            complement(2725..3435)
                     /gene="uppS"
                     /locus_tag="P5541_04360"
     CDS             complement(2725..3435)
                     /gene="uppS"
                     /locus_tag="P5541_04360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRV71245.1"
                     /translation="MKFENKKKPGFPAHIAIIMDGNGRWASLRGMPRVAGHQEGARAV
                     ERIIRAAAESGVKYLSLYAFSTENWKRPATEIEGLMGLFRYYLKGKLEDLKKNGVRLV
                     FSGRKAELPQDLVDEMQKAEKETLVGQKLTVVVCVNYGGRQEIADAFTRLLQIGTKGP
                     ISEKDISSALYLPEIPDPDLVIRTSGEMRLSNFLLWETAYSEMYFTDVLWPDFSEPDL
                     IAAIQDYSKRERRYGSVS"
     gene            complement(3432..4043)
                     /locus_tag="P5541_04365"
     CDS             complement(3432..4043)
                     /locus_tag="P5541_04365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRV71246.1"
                     /translation="MNENVQKINPSARWDDAWKKLMEETSSCDRCPLHKGRNRTVFGE
                     GSTLNRLMFIGEAPGADEDEQGVPFVGKAGQLLTGILDAAGIGRTEIFITNVVKCRPP
                     GNRVPAIEEMLACEDILKSQIALLNPRIIVCLGATPTKWILKTGDNISAIRGKWFDWK
                     GIQVMPMFHPSYLLRNPSRKKGAPKELTWIDIQEVRRKWKEDA"
     gene            complement(4061..4906)
                     /locus_tag="P5541_04370"
     CDS             complement(4061..4906)
                     /locus_tag="P5541_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71247.1"
                     /translation="MNEPRSSVDLSGVSVVAFVGPAGTGKSQRAQLVALEIGADLIID
                     DGLLIKKGQILRGKSAKTEKNQVRAIRRALFQYEDHRQSVVEILSESQPCTVMLIATS
                     DGMAKQILDNLGLSQPTRYVRIEDVSSPEEIFRAKKERTMKGQHVIPVSHIQVRKHFA
                     GKLVGRLKVLFDNADGMEGEKTIVRPPFKFFGEVIIDPAVVDQMARFIAERTAQVTAV
                     PFVQTRPSNGGVDIQIGLKCRPGERSFLTAGENLRRRISVAVSGITGLDVHRVDITVV
                     EVEQF"
     gene            complement(4899..6008)
                     /gene="hisC"
                     /locus_tag="P5541_04375"
     CDS             complement(4899..6008)
                     /gene="hisC"
                     /locus_tag="P5541_04375"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV71248.1"
                     /translation="MGWLKGIIRNDLDGIEKYKYGKPVSDLQRELGLEKVVRLCSNEN
                     PWPLPESVMKAIRASLEKVNFYPDPQSFRLRRMLSQKCGVSTGEVIIGAGTEGILHSM
                     LHSIIEPGDEIVCPIPAYPLYRVAAVAASARFVEVRSEDGNELDTGIILASCSERTKA
                     VIISNPCNPTGRIMERERLTALANELDSRQVMLIVDEAYAEYVDDPEYLAGIELFRNI
                     GRVVITRTFSKIYGLAALRVGYAIAPKTMVESYDKLHPVFEVSRIGQYAAHAAIQEDA
                     FIRDIRIRTIAERNRIFKNILDIGISAVNTTTNFLLIKHKKSDEIYELLLREGVIIRK
                     GSDLGMKGYLRITVGLPEENDIFLSSLKKVLTRIE"
     gene            complement(6035..7318)
                     /gene="murA"
                     /locus_tag="P5541_04380"
     CDS             complement(6035..7318)
                     /gene="murA"
                     /locus_tag="P5541_04380"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRV71249.1"
                     /translation="MNYKMLIHGGATLKGSVEVQGAKNAALPVIAASLLLKDKTLKIR
                     RVPKLQDVFTMAELLRHLGAKVDFRGNDMSIAVPDDLAWETPKTLVRKMRASSLVLGP
                     LLARTGRAILPLPGGCAIGSRPIDLHLKGLAKLGASIDLVHGAVHATAKGLKGAKIYL
                     DFPSVGATENLLMAAVFAEGETIIENAAREPEIANLVETLGLMGCEIDGAGTGTIRIT
                     GTGDLEPAEVEVIPDRIEASTYLIAGAATRGDVTVKKVISEHLDAIIAKLEETGVGVE
                     IMEDSVRVFPRSPLRSVSLKTLPYPGFPTDVQPQFSALMSTTEGTSIIQESVFESRFL
                     HVSELKRMGANIEVQGNTAVVTGVPRLNGADVKATDLRAGAALVIAGLSSAEDTSVFG
                     LGHIWRGYEGMDEKIRNLGGDVHIVISEENGDNKW"
     gene            complement(7359..8489)
                     /gene="wecB"
                     /locus_tag="P5541_04385"
     CDS             complement(7359..8489)
                     /gene="wecB"
                     /locus_tag="P5541_04385"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRV71250.1"
                     /translation="MNKEIVCCVVGTRPEAIKMAPVILQIAKSKCLQPFVLATGQHTE
                     MLTQPLDFFGISPDVNLSIMRDSQTLDYITASVLEKTGSVFDEIKPFMVLVHGDTTTT
                     MAASLAAFYRKTRLGHVEAGLRSGNINLPFPEELNRIVSDRVSGWWFPPTTRAMERLI
                     AEGCDPSRVIETGNTVIDALVWASGKVEKPGIPQLEKIPAGARVMLMTAHRRESWGEP
                     LEGICETVSRITEIYPDLWVIVPMHKNPQVREVIKSVLGGNERVLLCEPLDYPDFVWA
                     MNRSAVILTDSGGVQEETTYLRIPTLVMRDVTERPEALEYGTSFLVGTDPVRIRKHVS
                     RVLEVDSIFERTVEETKPPFGKGNASEKIVETLEEILRTGSR"
     gene            complement(8497..9393)
                     /locus_tag="P5541_04390"
     CDS             complement(8497..9393)
                     /locus_tag="P5541_04390"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRV71251.1"
                     /translation="MVGNPLIFITFFLLALVVTPLSIAVSRQFGIIDEPGGRKTHNKS
                     TPRGAGVVIWLGYMLFSLFFPDSGNGLRLISTAATVIFIFGYMDDMRPLPATWRLCVH
                     GLCSAVVVAFLPVTPVFKLILFLWITGSTSAYNLIDGINGLSLILFALTVILGLFLFG
                     QYWWAALLGLAAGVLPWNYPVARTFLGDGGSTLIGFICMSQFSSELASYSTEMSVAGF
                     LFLLLLIGGVPVIDTLYSIFRRLLAGVSPFLPDRGHFHHILCDRGFPVVVVLLVLGIL
                     HSFLLLGAAVILGHPLPFHGVM"
     gene            complement(9425..10525)
                     /gene="upp"
                     /locus_tag="P5541_04395"
     CDS             complement(9425..10525)
                     /gene="upp"
                     /locus_tag="P5541_04395"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRV71252.1"
                     /translation="MRIALGSDHAGYSLKKCIASFLIENTSFSVGEFGAANAEDPYDY
                     PDAAFDVGKRVSSGEFDLGILVCGTGIGMVMAANKIPGVRAALAYDEDTARFARRHNN
                     ANILGLGGRKTDCERALKIVKEWLSQDFEGGRHRNRIDKICSFEMDNVRNAGTEVSPR
                     LVTFDHPLIQHKLGMIRDKTTSVKVFRELVQEIAGLMVYETTRNLPLEPVEVETPITR
                     TTAFSLSGKKIAIVPILRAGLGMVDGVLQLIPNAKVGHVGLYRDPETLEPVEYYCKLP
                     GDIGERDIIIVDPMLATGGSASAAIDLVKRKGGVKISLLSLIAAPEGVSRVHGKHPDV
                     DIYTAALDSHLNDHGYIVPGLGDAGDRLFGTR"
     gene            complement(10619..11557)
                     /gene="meaB"
                     /locus_tag="P5541_04400"
     CDS             complement(10619..11557)
                     /gene="meaB"
                     /locus_tag="P5541_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRV71253.1"
                     /translation="MDKIVGKALGGDQRSMARLITLVENEAPGATDVMKAVYPHSGKA
                     HIIGVTGSPGAGKSTLLDKLINGITGSGHDLGVIAVDPSSPFSGGAVLGDRLRMQNHT
                     PGANVFIRSMATRGSLGGLSRATYEAALIMDACGKDVVLIETVGVGQSEIDIMKIADT
                     VALVLVPGMGDDIQIMKAGIMEIADIFIVNKADREGYERIVREVNIMLDLLGDKPWRP
                     PVIPTIAEKGDGVDVAYGAIENHRNYLESSGEGKKRKFDRLRLEVEEILRREIAKVAE
                     KAWDRKISSHILEDLAARRNDPYRVTEEILSNILLS"
     gene            complement(11618..11833)
                     /locus_tag="P5541_04405"
     CDS             complement(11618..11833)
                     /locus_tag="P5541_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HRV71254.1"
                     /translation="MPVANIQALSALGLFLACLFISRMIVNISSGKWPGGIMWVTYLR
                     TLLGFMFAAAITLALYAFAGIEFLKRN"
     gene            complement(11991..12962)
                     /locus_tag="P5541_04410"
     CDS             complement(11991..12962)
                     /locus_tag="P5541_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRV71255.1"
                     /translation="MDPVRVGVIGVGHLGYHHARVYTELLNTDVVGIVDSNPDRAAAV
                     GELLRVPYYSDIEKFYSQARPDAVSVVVPTVYHYETAKKALSSGVHVLIEKPVTSTVE
                     EAEELLKLAATHNLVLQVGHIERFNSAVQHVRNIIKAPLFLQSRRIGPFSSRINDVGV
                     VLDLMIHDIDIILSLVHSEISNISAMGRSIRSSFEDIASAQISFENGALAQILVSRVS
                     ERRLRQLEIMEPERFVTINYETQDVSIHRCIQEKECGLVEVIEHPVFPKREPLKLELQ
                     HFVTCVREGRQPLVGIMDGKRALEVAIAILKQIQTPQRSAGEKIGLA"
     gene            complement(13010..13813)
                     /locus_tag="P5541_04415"
     CDS             complement(13010..13813)
                     /locus_tag="P5541_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRV71256.1"
                     /translation="MTLEHEKWKLAVSGKQSLIRFIPPGDMSKRMSVTLFLRGPVTEG
                     AEGSPEKAASLLFEDPVKTTFVAPLQRHGTAVIDAVPIWSLPARPEADSVFISRSGIW
                     GSLRFADCFPVIVVSEKPFDWCLLVHSGFAGTCRGAVSKGLRKMLSTVGMARAEECHA
                     WIGPGIGGCCYSRSLEDPSTMRAMACLPGDSFRVENGRVYFDLRNAIITQLYDMGIPI
                     NNITDTGICTSCSHPLCFSYRKGDRNARSLLLARLYSSYHKQPFWWENV"
     gene            complement(13832..15052)
                     /locus_tag="P5541_04420"
     CDS             complement(13832..15052)
                     /locus_tag="P5541_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV71257.1"
                     /translation="MARLMSALGNPERNFQAVHVAGTNGKGSISAMLESVFLKAGYRT
                     CLYTSPHLVDITERIRFCGIPSSKEIFLDSVKEVCSAVRSNFDQKNRPTYFEVLTAAA
                     FHSIARYAPDIAIVEAGMGGRLDATNILKGVVMSVIATIGMDHSEFLGPDLGAIAMEK
                     FLVLRPGGISVFSGGNSELESRYDQLCERISNKGLVASREACIKDPETGVDRNSFLLA
                     TGNMELGRFTTSLGGIFQLENARTAILAALGLADKYSGINLSTVKSGLSSTDWPGRLE
                     KVSFRGRELILDGGHNLEGVAGTTETLRLCGYSNNCAVVFAAMKDKDLQGMIRKIVEN
                     FPFVVFTSVPGTARSANPWNLLDMARTMNTETFLDVEEEPMKAIEKAYGFAGRILCCG
                     SLFLVGEVKKQIRG"
     gene            complement(15152..17824)
                     /locus_tag="P5541_04425"
     CDS             complement(15152..17824)
                     /locus_tag="P5541_04425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRV71258.1"
                     /translation="MATDGKTLGKSYDPGPIEEKWYGRWTEGGLFRGDAESQKPSYCI
                     VIPPPNVTGSLHMGHALNNTLQDISCRYMRMKGKNVLWLPGTDHAGIATQNVVEKNLS
                     GKGISRHDLGRDAFVEKIWEWKKEYGSRIINQLKRLGASCDWERERFTMDDGLSRAVR
                     SVFVRLYKEGLIYRGKYIINWCPRCQTALSDLEVEHHEEKGKLFHVRYPLEGEEGYLV
                     VATTRPETILGDVAIAVHPRDEKNSSLAGKFVKVPVTGRVIPVIEDNMVDPAFGTGLV
                     KITPAHDPNDFLVGNRHGLEAIQVIDEHGVMNEEAGAPFTGLDRFEARTRMVNVLEKE
                     DLLEKIEEHDNAIGHCYRCDCIVEPYLSEQWFVRTEPLAKPGIEAVRDGRINIVPSQW
                     ESTYNMWMENIRDWCISRQLWWGHRIPAWTCSDCGHLTVSEEDPTACEACGSSRICQD
                     EDVLDTWFSSALWPFSTLGWPDKTKELETFYPTSLLVTGFDILFFWVARMIMMGLRFM
                     DKEPFTDVYIHALVRDEHGQKMSKSRGNVIDPLEIIEKSGSDSLRMTLAALTVQGRDI
                     FLGPERIEVYRLFMNKIWNASKFALANLDGEKCDPFKEGTALRTHDRWILERLAVVTE
                     NVTRDLDGYFYGEAARSLYQFVWNEICDWYLEMAKPSLRGDEGEDRGMAARAVLELVF
                     SNIFRLLHPFIPFLTEELWEKFGFGNDFIEKEEWPVPKHVPGGSSSVDEMEIFQEAVR
                     NARNLRAEARMHPQQEIREVVINLSDKAGVSAEVLGNNADLFCLLVRTAVLRVNPPGS
                     TRPPRSLMAVCGFGEVFLVAGDLLDAVSEVTRLEGELAKAKKDLERSTAKLSDSSFIS
                     RAPAEIVEKEKARVSASEEKILLIERNIESLSRQ"
     gene            complement(17903..18517)
                     /gene="yihA"
                     /locus_tag="P5541_04430"
     CDS             complement(17903..18517)
                     /gene="yihA"
                     /locus_tag="P5541_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRV71259.1"
                     /translation="MMVERMPQGKYKAEIMATSFNSKQFPEPFLPEIAFAGRSNVGKS
                     TFINGIIQTRLAHVSSTPGKTRSINFYRVERKKGFTLVDLPGFGFARRGKAERKAWGD
                     LIGEYVQGRESLVLLVHLVDFRHGMLDNDILFQEWIKKTGVPVQVVYTKIDKLSRGNW
                     AQLKARYSAKSFSSVSEPILVSMEKGWGTGEFHLFLESYLDSLE"
     gene            complement(18483..20831)
                     /gene="lon"
                     /locus_tag="P5541_04435"
     CDS             complement(18483..20831)
                     /gene="lon"
                     /locus_tag="P5541_04435"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRV71260.1"
                     /translation="MLPLLPVRDLVLFPGVIVPLFVGRPRSLKALEEAMLHEKTLLVV
                     AQKEMSVEDPEPEDIYGAGTITTIIQMLRLPDGTTKVLVEGISRAAINSVALENDTFF
                     AEIEPLEGSSIPAGPETEALGRTLLAQFERYVTLHPKIPLEVMMSVSSLEDSGQISDL
                     VASHLTVKIAERQQILEASDIPQRMEMILKILLREVELLEMERSIHDRVRQEMEKGQK
                     EYYLREQLKVIQDELGQGEDFSEMEAFREQINTSRMPEDVRKKALHELDRLGKMPPMS
                     AEATVVRSYLDWMTSLPWRKSTRDSVDVTLSSKVLDEDHYALEDAKERILEYLAVRKN
                     AGSRLKGQVLCFTGPPGVGKTSLGRSIARATGRKFVNMSLGGIRDEAEIRGHRRTYIG
                     ALPGRIIQKMRQAGTSNPVMLLDEIDKIGLDFRGDPASALLEVLDPEQNNAFTDHFLE
                     VPYDLGSVLFITTANSTHTIPKPLLDRMEVIRLPGYVNEEKIQIARRHLIPKVLQEHG
                     LKGEQISFTNSSLARIISDYTREAGVRELERKISRIARKITRKIVESEEKKNKNSKKS
                     FSVGVSSLVSLLGVPRHLGGVHLPSSSQRGAAIGLAWTETGGDILVIESAMITGKGKI
                     LLTGNLGDIMQESGQTALGYLKAHAGDLCISSIDWDKTDIHVHVPEGAIPKDGPSAGI
                     TLAVSIYSALSGIPVRSGLAMTGEVTLRGEVLPVGGIREKILAAKRYGVFNVILPADN
                     RMETKEMPGWVLKGMNLLFVDKLDDVFRSALDDGGTDAPGKV"
     gene            complement(20862..22139)
                     /gene="clpX"
                     /locus_tag="P5541_04440"
     CDS             complement(20862..22139)
                     /gene="clpX"
                     /locus_tag="P5541_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRV71261.1"
                     /translation="MSGTTGNGSRKTKREARCSFCGKSQDEVTKLIAGPGVYICNECV
                     NLCNLILRENEDGKSLPVPFEKEFRDLPKPVEIKEQLDQYVIDQEQAKKVVSVAVYNH
                     YKRIACGIEDDLEMQKSNVLLIGPTGSGKTLIAETLARTIRVPFAMADATTLTEAGYV
                     GEDVENILVRLLQAADYDIAAAERGIIYLDEIDKICRKSESASITRDVSGEGVQQALL
                     RILEGTEANIPPKGGRKHPHQEFIQINTKNILFICGGAFDGLENIISRRTNRKIIGFG
                     GQVSRNEKKNRFRVLKDVQPDDLMSFGFIPEFVGRVPVTTALEGLDEKALVRILTEPK
                     NALVRQYQKAFRMENVTLEFTAEALAAIARKACERNTGARALRSIMESAMLDLMYELP
                     SRNDGLSKVVVTDGMIEGKEPPVLDYARDGEVA"
     gene            complement(22291..22869)
                     /gene="clpP"
                     /locus_tag="P5541_04445"
     CDS             complement(22291..22869)
                     /gene="clpP"
                     /locus_tag="P5541_04445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRV71262.1"
                     /translation="MFIPMVIEQTGRGERAYDIYSRLLKDRIIFIGDAIDDHLSNLVV
                     AQLLFLESEDPDKDVNVYINSPGGVITSGLAIYDTMRYIKCPISTICVGQAASMAAVL
                     LAGGDKGKRIALPSSRIMLHQPLGGTQGQAVDIQIHAREIMRLKDRIHEILVAHTGKT
                     ESEISADTDRDFFMSAEEAVSYGIIDKVIAQR"
     gene            complement(22869..24212)
                     /gene="tig"
                     /locus_tag="P5541_04450"
     CDS             complement(22869..24212)
                     /gene="tig"
                     /locus_tag="P5541_04450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRV71263.1"
                     /translation="MRSEIISQEKNKVKILIEVQEETLAKRVEETTEELRAKANIKGF
                     RKGKVPRKVLELHLGMDYIRTEAIEKMLPEYLDSVVKEYELDLIADPSLEIDTVEAQS
                     PLRITATFEVRPEVTIPELDTVKVIAKTFKVTDTMVDEAIERMRERHASTLPVSDRSS
                     HNGDIVELEYSVVLEKNTKSLDEGDRKHQKGAVEIGSDSLDPAISNALEGKNAGDEVE
                     AEVMLKAEGEEEGKKALYRMKILSVSEKVLPELGAEFFEKITGEKDITAEVFRERVAK
                     GIQDNFDRDCRELTENNAIEAVAASSEVDVPESLVKIQKEENLKQIRDNIKNKTGKEL
                     EEYISANGLEMEFIEKDAEESAANIVKRSLVMEAVADKENIIIEKGDIETEIANMAEA
                     FRVDVDQMRQFYLKKNKDLSELIHKVRIRKTVARILEKAEIQEEEIMVDQKEAKA"
     gene            complement(24339..24423)
                     /locus_tag="P5541_04455"
     tRNA            complement(24339..24423)
                     /locus_tag="P5541_04455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24387..24389),aa:Leu,seq:tag)
BASE COUNT         5982 a         6569 c         5438 g         6496 t
ORIGIN      
        1 ttcctgttac ccattgaagg ataccccgta ctatgtcaag gctgaaattc catatgtagc
       61 ctatagatcc tgtaaaggcc cggcccaggg gatacttttt tatggagggc ctgataccga
      121 gaagatacgt ctggttaatt gggtcgaagg gtatctccag gaaaatttcc tttatttccc
      181 catccctttc aaaaaggatc aaaacaggcc cttccacgga tcgttccctt attgccgatg
      241 aaagttcggt ccagtcggaa acaatactcc cggatacggc ttcaattctg tcccctgcaa
      301 cgagtccagc tttatcagcc ggataaccgg ccatgatctc accaattcgt gtgctggaaa
      361 gatccagtac cccgtgcccc cagagaagaa tagcagtaat aagaacggcc agaaaaatat
      421 tggaggcaga tcctcctgcc agaatggcaa gtcttttcca cgcagatttt tcctggaatg
      481 atcctccggg ggggattact tcatcggtct gctcctcacc cattccggcc agtctgacaa
      541 agcctcctac cgggacagct ctcaaggacc agagtgtgtc ctttcgtttg aaacttgcca
      601 gagatggacc cattccaaaa gagaattcgt gaaccttcac tccgcaacgc cttgcaatga
      661 agaaatgccc agcctcgtgt gaaagaacgc atatcgctat tacgagaaca aatgccaaca
      721 agcttgtcac catcattttt ctctccccat gcccctcatg tcagaggata tcttctctaa
      781 aacactcttc ctgcccattt ccacaagata aagggcttct tcccatgatc ccggagcttc
      841 tccggaaaag gattccagtg cctcctggat gaatagggga atatccgtaa accctattct
      901 tcccttcata aaaagatcga cagccgcttc gtcagccccg ataagcaagg gtggatatgc
      961 accccctagt cggcatacct ccttcgcgat cttcaggcag ggaaatttat tttcgtcggg
     1021 ctgccggaat tccagtaaga gttcttttgg ttccggcggg ttcaggttta cgtcagtgtt
     1081 ttgcagtctt ccagaataac caagagcggc aagagcggca agtctcatat caggcctgga
     1141 aaggagcatt ttaacggacc cgtcaataaa ctctaccaga gcatgaactt gagatcctgg
     1201 atgtatgacc gcatcaattt tttcgggtgg caatgagaag agctgtctgg cctcaatgat
     1261 ctctatgcct ttgttcataa gggttgcgct gtcaacggaa atcttgctgc ccatgggcca
     1321 gaccgggtgc cggatggcca tttcgaaggt taccactgac agttcttcat caggcaattc
     1381 gagaaaaggc cctccggaag cagttagaaa aatttttctt acgttttccc tgtcttctcc
     1441 cttcagagac tgccagacag cattatgttc actgtcaaga ggtatgaggg ctccggctcg
     1501 ggcataaggc ataacccatt ctccggctgc gactacgatc tccttgtttg caagaaatat
     1561 ttttttaccg gccttcaagg ccattacaag cgctggaagc gcatctgttc cggaagaagc
     1621 aaaaaccacc tcttcacatc gggggtcttc aattatcgat tcaattgcac ctgcgccccc
     1681 gcggcattcg aaactgactg accccttgtt ttgtagttcc atcgcagcca tttcgtcgga
     1741 gagaaccaaa agatccgatt cgaacttttt cccaagtgag agaagtcctt caacgttttt
     1801 agcggcggca agagcccata cattaaaaat gtcataatgc atcaggcagg cctcaagcac
     1861 ggctcctcca acacttccgg ttgccccgat tacggcgatg tttctttttg ttccgatcat
     1921 catcagagga ctccaaaaaa ggcaaaaacc agcacagaac tgaccagaat gctgtcaaaa
     1981 cggtcaagga agccaccatg acccggtatg aggtccccag aatccttgac tcccgcttct
     2041 ctctttatta gagattctgc aagatctccc gcctggccga aggttccgca tatcagggcg
     2101 ataagaacaa gcggaagagg cgctaccccc caaaggaaag agagccctcc gccgcagaga
     2161 attgatcccg ctaagcctcc ggcaaagccc tcgatactct ttttcgggct gacagatgga
     2221 cacagtcggg tttttcccca gtgagaaccg accagatatg ctgcaacatc gcaggaccac
     2281 gtacaggcaa aaagcgcagt cagaagatac ataccccata tgtgatccct cagaattacc
     2341 agaaaggacc atggaagaac aatataaagt aacccggccg gtgtcgcaca ggaacttgct
     2401 atcccgtgac ttgagccggt aagatgcctt ctcgctattt ctataaataa cactgcgagc
     2461 ggaacaaccg ccagtatggg taaaattaca ctactatcgg atgaaaaagc tgctgccgta
     2521 accaggaaaa atgccgcagg ataaccggtg atcctggata catggaatct gaaacctgtg
     2581 atcctgtaga actctgaaag tgaaagaatt gaaatacata aacatattgc gtaccaccat
     2641 gggttaccaa ggtagattcc gccgagaact gcaaggcaga ttacagcacc actgacgctt
     2701 cttgtaataa aaccatttga agaatcagga gacagagcca taacggcgct cccttttcga
     2761 ataatcctga atggcggcaa tgaggtcggg ttcgctaaaa tcgggccaaa gtacatcggt
     2821 gaaatacatt tcactgtaag ccgtttccca aagcagaaaa ttactgagcc tcatttctcc
     2881 acttgtcctg ataacaaggt caggatcagg aatttccggg agataaaggg cagagctgat
     2941 gtccttttcc gaaattggtc ctttggttcc aatttgcaga aggcgggtaa aagcgtcggc
     3001 gatctcctgt cttccgccat aattaacgca caccacgacc gtcagttttt gtcccacgag
     3061 ggtctctttt tcggcctttt gcatctcatc aacaaggtct tgaggcagtt ccgctttcct
     3121 gcctgaaaag actaacctta caccattttt tttaagatcc tcgagcttgc ccttgaggta
     3181 atacctgaaa aggcccatga gtccctcgat ctccgtggcg ggtcgcttcc agttttctgt
     3241 agagaaggca taaagggaaa ggtattttac accggattcc gccgccgccc ttattatcct
     3301 ttcaaccgct ctggccccct cctggtgccc ggcaaccctt ggcattccgc gcaatgaagc
     3361 ccaacgccca ttgccatcca ttattatggc tatgtgggcg ggaaacccgg gttttttctt
     3421 attttcgaat ttcatgcatc ttccttccat ttcctgcgaa cttcctggat atcgatccag
     3481 gtaagttctt tgggagcgcc ttttttcctg gaagggttcc tcagaagata gcttggatgg
     3541 aacatgggca ttacctgtat tcccttccag tcgaaccatt ttccccgtat cgccgaaatg
     3601 ttatctccag ttttgaggat ccacttcgtg ggggttgccc caagacagac aatgatcctg
     3661 gggttcagca gggctatctg tgatttaagt atgtcctcac aggctaacat ttcttcgatg
     3721 gcagggacac ggttgcccgg tggtcggcat ttgaccacgt tggttatgaa aatctcggtt
     3781 ctgcctattc cggcggcatc cagtattcca gtaagaagct gacccgcctt tccgacaaaa
     3841 ggcacaccct gctcatcctc gtccgctcca ggggcttcgc cgataaacat taacctgttc
     3901 agggttgagc cttccccgaa aacagtcctg ttccggcctt tgtggagagg gcaccgatcg
     3961 caggaagatg tttcctccat cagttttttc catgcgtcat cccaccttgc ggaaggattt
     4021 attttctgaa cattttcatt cataattttg ctctcccctg tcaaaactgt tcgacctcaa
     4081 ctaccgtaat atctacacgg tgcacgtcca aaccggtaat acctgaaacg gccacggaaa
     4141 ttcttcttcg aagattttct cctgcggtaa gaaaggatct ttccccaggt cggcatttca
     4201 ggcctatctg gatatcaact ccaccattgg aagggcgagt ctgtacaaaa ggaacggcgg
     4261 ttacctgggc tgttctttca gcaataaacc tcgccatctg gtcaactaca gcggggtcga
     4321 taataacttc accgaaaaac ttgaacggtg gacgcacgat ggtcttttct ccttccatac
     4381 catcagcgtt atcgaaaaga accttgaggc ggccaaccag tttcccggca aaatgtttac
     4441 ggacctgaat gtgggatacg ggaataacgt gttgtccctt catggtccgt tccttcttcg
     4501 cacggaatat ttcttccgga gatgaaacat cttcaatacg aacatatctt gtcggttgag
     4561 aaaggccaag gttgtccagg atctgtttgg ccattccgtc ggaagtcgct ataagcatca
     4621 ctgtacaggg ctgagattca gagagaattt ccaccaccga ctgccgatgg tcctcatact
     4681 gaaagagggc tcttcttatc gccctgacct ggttcttctc ggtctttgcg cttttccccc
     4741 tcagtatttg cccctttttg atcaaaagac catcgtcaat tataagatcg gctccaattt
     4801 ccagagccac gagctgggct ctctggcttt ttccggtacc ggctggtcct acaaaagcaa
     4861 caaccgagac cccggaaaga tctacagaag atcggggctc attcaatccg ggtcaacacc
     4921 tttttaagcg atgaaaggaa aatatcgttc tcctcgggaa gcccaacagt tattcgaaga
     4981 tatcccttca ttcccagatc gctaccctta cggataatta caccctccct caacagcagt
     5041 tcgtagatct catcactctt tttgtgttta atgagaagaa aatttgtcgt tgtatttaca
     5101 gcggatattc caatatccag tatgttttta aagatcctgt tcctttcggc aattgttctg
     5161 atccgtatat cccgtataaa ggcatcttcc tggatcgctg cgtgggcagc gtattggccg
     5221 atcctgctga cctcaaatac agggtgaagc ttgtcgtagg attccaccat tgtctttggc
     5281 gctatagcgt aaccgaccct caaagcggcc agtccgtaga tcttggaaaa agttcgtgta
     5341 ataacgaccc ttccaatatt cctgaacagt tcaatgccgg caagatactc cggatcatcg
     5401 acatattcgg cgtaagcctc gtccacgatc agcatgacct ggcgcgaatc gagttcgttg
     5461 gcgagggctg tcaacctttc acgttccatg atccggcctg tagggttgca gggattggat
     5521 attataactg ccttcgtcct ttcgctgcac gaggccagga ttattcctgt gtccagttca
     5581 tttccatctt cggaccggac ctccacgaaa cgtgcggaag ccgccacggc agcgaccctg
     5641 tacaggggat acgctggtat tgggcaaact atctcatccc ctggttcaat aattgaatga
     5701 agcatcgaat gaaggatacc ttccgtgcct gcgccaatta ttacctctcc cgtagacact
     5761 ccacacttct gggaaagcat cctcctgagc ctgaaagact gaggatcggg ataaaagttt
     5821 accttctcga gacttgccct tatggctttc attactgatt cgggcaaagg ccacggattc
     5881 tcgttggaac aaagccttac cactttttca aggcccagtt ctctttgaag gtcgcttaca
     5941 ggcttcccat acttgtactt ctcgataccg tcaagatcgt ttctgattat tcccttcagc
     6001 caacccaact ctcatacccc gtctctgtcg gaaattacca tttattgtcc ccgttctctt
     6061 cggaaataac tatgtgtaca tctcctccaa ggttgcggat cttttcatcc attccctcgt
     6121 aacctctcca tatatggcca agtccaaaaa ctgaagtatc ttccgccgat gaaagccctg
     6181 ctattaccag agcggcccca gcccgaagat ccgtagcctt gacatcggct ccgttaaggc
     6241 ggggcacacc agtaacgacc gctgtattac cctgcacctc tatattggct cccatccttt
     6301 ttagttcact gacatgcaaa aagcgggatt caaatacact ttcctggatt atgcttgtcc
     6361 cttctgtggt tgacataagt gccgagaatt gaggctgtac atccgtcggg aaaccaggat
     6421 aagggagcgt cttcagcgaa acacttctta aaggcgatct tggaaagact ctgacgctat
     6481 cttccattat ctccacacct acacccgttt cctccagctt ggcgataatg gcatcaaggt
     6541 gctcagatat aaccttttta accgtaacgt ctcccctggt cgcggcccca gctataagat
     6601 atgtgctggc ttctattctg tcaggaatta cctctacttc cgccggttcg agatcgcccg
     6661 ttcctgttat ccgtatagtt cctgtacctg cgccatctat ttcacaaccc atcagcccaa
     6721 gagtctcaac aaggttggcg atctcaggtt ctctggccgc attctcgatt atggtctctc
     6781 cctcggcaaa tacggccgcc atgagaaggt tctcggtagc gcccacagaa ggaaaatcaa
     6841 gatatatctt ggcccccttc aagcctttgg cagttgcatg aaccgcccca tgaacaagat
     6901 cgatactggc accaagtttt gccagcccct tcaggtgaag gtcaatgggt cgacttccta
     6961 tagcgcaacc acccggtaga ggaagaatgg cccttccggt tctggcaagt agcggaccaa
     7021 gaacaaggga ggaagcccgc atcttcctta ccagcgtctt tggagtttcc caggcaaggt
     7081 catcgggaac ggctatactc atgtcgttcc ctcggaaatc caccttggcc ccaaggtgac
     7141 gaagaagttc agccatggtg aaaacatcct gaagtttggg aaccctcctg atcttgaggg
     7201 ttttgtcctt caggagcagt gatgctgcga tcacaggcag tgctgcattt ttcgcgccct
     7261 ggacttcgac ggagcctttc aaagttgcgc caccgtggat aagcattttg tagttcaaga
     7321 gatcacctcg tgccattcag gcttgaaata tttcaggatc atctggagcc ggtcctcaaa
     7381 atctcttcca gagtttccac tatcttctcc gatgcattgc ccttgccgaa cggaggtttg
     7441 gtttcttcca cagtcctttc aaaaatgctg tcaacttcca gtacccggga cacatgtttc
     7501 cggattctaa caggatcagt tccgacaagg aaagacgttc catattcaag cgcctctgga
     7561 cgttcggtaa catctctcat taccagagtc ggaatcctga gatacgtcgt ctcctcctgg
     7621 acacctccgc tatcagtcag aatgacagcg cttctgttca ttgcccagac aaaatcagga
     7681 taatcgagag gttcacatag aaggaccctt tcatttccac caagtactga tttgataacc
     7741 tctctgacct gggggttttt atgcattgga acaatgaccc acagatcagg ataaatctct
     7801 gtaatacggg aaacagtttc acagattcct tcaaggggtt ccccccagga ttcccttctg
     7861 tgggcagtca tgagcattac ccttgcacct gctggtatct tttcaagttg aggtattccg
     7921 ggcttttcaa ctttaccgga tgcccataca agagcatcga tgaccgtatt tccagtctct
     7981 atgactcttg acggatcgca accttcggca ataagtctct ccatggctct cgtcgtcggc
     8041 ggaaaccacc atcctgaaac cctgtcagaa actatccggt taagttcctc gggaaatgga
     8101 aggttaatgt tcccgctcct caggcctgcc tcaacgtgtc caagccttgt ctttctgtaa
     8161 aaagccgcaa gggaagccgc catggtcgtt gtggtatctc cgtgtacaag aaccataaaa
     8221 ggttttattt catcaaagac actgccggtt ttttccagaa ctgaagctgt tatgtagtcc
     8281 agggtctgac tatccctcat tatagaaaga ttaacatcgg gacttatacc gaaaaaatcc
     8341 aggggctggg taagcatttc ggtatgttga cctgtagcaa gaacaaaggg ttgaaggcat
     8401 ttgctctttg cgatctggag aatgacagga gccattttta tagcctctgg tcttgtcccg
     8461 actacacagc agacaatttc cttgtttata ttcacattac atcactccat gaaacggcaa
     8521 gggatgaccc aatataacgg ctgctccaag gagcaggaaa gagtgcaaga taccaagaac
     8581 aagaagcacg acgaccacgg gaaaacccct gtcgcaaagg atatggtgaa agtgacctct
     8641 atcggggaga aaaggggaga ctcccgccag cagccttcgg aagatagagt aaagagtatc
     8701 tataaccgga acccctccga tcagaagcag taaaaaaaga aatcccgcaa cagacatttc
     8761 cgttgagtag gaggccagtt cactggaaaa ctgggacata cagatgaagc ctatcagggt
     8821 gcttcctcca tcgccaagaa atgttcttgc tacgggatag ttccatggga gtacccctgc
     8881 tgccaatcca agcagcgccg cccaccagta ctgtccgaaa aggaaaagac cgaggatcac
     8941 ggtcagcgca aagagaatca gtgaaaggcc attaatcccg tcaataaggt tataggcact
     9001 cgttgaaccg gtgatccaca ggaaaaggat caacttgaac accggtgtaa caggaaggaa
     9061 ggctaccaca actgcagagc ataaaccatg cacacaaagc ctccaggtgg ccggaagagg
     9121 tcgcatatcg tccatatacc cgaatatgaa tatcaccgtc gcagctgttg atatcagtct
     9181 gagtccgttt ccggaatcag gaaaaaaaag ggaaaacagc atgtagccaa gccatatgac
     9241 cacaccggcc ccccttgggg tagatttatt gtgggtcttt cttcctcccg gctcatcaat
     9301 tattccaaac tgtcttgaga cggcgataga gagcggtgta accacaagag ccagaagaaa
     9361 aaacgttatg aaaattaacg ggttcccgac catgtcagag gggttctctc atttttcagg
     9421 acaactatct tgtcccgaaa agcctgtcgc cggcatctcc gaggccgggt actatatatc
     9481 cgtgatcatt aagatggctg tccagtgcgg cagtataaat atcaacatcc ggatgttttc
     9541 cgtggaccct tgaaactcct tcaggggccg cgatcagact caggagagaa atcttgaccc
     9601 cacccttcct cttgacaagg tcgatcgccg cggaagcgga tcctcccgtc gcaagcattg
     9661 gatctacaat gattatatcc ctctcaccga tatctccggg gagtttgcaa taatattcta
     9721 ccggttcaag cgtttcggga tcacgataaa ggcccacgtg tccaaccttt gcgttgggga
     9781 tcagttgcag gacaccatcg accattccga gaccggccct gagtatggga acaatggcga
     9841 tttttttccc ggacaacgaa aatgctgttg ttcgggttat gggcgtttct acctccaccg
     9901 gctcgagagg aagattcctt gtagtttcat aaaccattaa tcccgcgatc tcctggacaa
     9961 gttcgcgaaa aaccttgaca gaggtggtct tgtccctgat catcccgagt ttgtgctgga
    10021 taaggggatg atcgaaggtg acaagccggg gtgatacttc cgttcctgca ttcctcacgt
    10081 tatccatttc gaaggaacat atcttatcga tccggttcct gtgtcttcca ccttcaaaat
    10141 cctgggatag ccactctttt acgatcttca atgctctttc gcagtcagtc ttccttccac
    10201 ccagacccag tatgttggca ttgttgtgcc ttctggcaaa gcgggcagta tcctcatcat
    10261 aagcaagagc tgcccgaaca ccggggatct tgttggcagc cataaccatt ccgatgccgg
    10321 tcccgcaaac cagtattccc agatcgaact ctcccgaaga gacccttttc ccgacatcaa
    10381 aggctgcatc cgggtagtcg tatggatctt cggcgttggc tgcaccgaac tccccaaccg
    10441 aaaatgatgt gttttcgata agaaaagagg ctatgcattt tttcagggaa tatccggcat
    10501 gatcggaacc cagagcgatt cgcatttttt gtcctccagt catttgtatc tgatgttttt
    10561 cgtatgatag catgttccgc cgtcaggaat gacggatcga tactctcctt cgtacttctc
    10621 aggataagag gatgttagac aggatttctt ctgtaaccct gtagggatcg ttccttctgg
    10681 ccgcaaggtc ttcaaggata tgggatgata tttttctgtc ccacgctttt tcggccacct
    10741 ttgctatctc tctcctgaga atctcctcta cttcgagcct aagtctgtca aactttcttt
    10801 ttttgccttc tccggaactc tccagataat tcctgtggtt ttcgatggca ccataagcca
    10861 catccactcc atcgcctttt tcagctatag tcggaatgac aggaggcctc cagggtttat
    10921 ctcccagcag atccagcatt atgtttactt ccctgacaat tctttcatac ccttccctgt
    10981 cagccttgtt aacaataaaa atatcggcga tctccattat acctgccttc attatctgaa
    11041 tatcatcacc catgcccgga acaagtacga gagcgactgt atcagcgatc ttcatgatat
    11101 ctatctccga ttgtcccact cccaccgtct cgataagaac tacatccttt ccgcacgcat
    11161 ccatgatcaa tgcagcctcg taagtggccc tgctaaggcc tccgagggaa ccccttgtcg
    11221 ccatgctacg gataaacaca tttgctccgg gagtgtggtt ctgcattcta agcctgtctc
    11281 ctaggaccgc tcccccggaa aaagggcttg aggggtccac ggctataacc cccaggtcgt
    11341 ggcccgaacc agtaattccg ttgatcagtt tgtcaagcag agtgctttta ccggccccgg
    11401 ggcttccagt aactccaatg atatgagcct tgcccgaatg gggatagaca gctttcatga
    11461 catctgttgc ccctggtgcc tcattttcca caagggtgat cagccttgcc attgaacgct
    11521 ggtctcctcc cagggctttt ccgactattt tatccaagat aaaaacaatc ctttcatgaa
    11581 aagttcgggg ctaaaagcat gtggtttctt atagccttca gtttcttttg agaaattcga
    11641 taccggcaaa tgcataaagc gccagagtga tcgccgcggc gaacataaat cccagaagag
    11701 tcctcaggta ggttacccac ataatacctc cgggccattt tcctgatgat atgttcacga
    11761 tcattctgga aatgaaaaga cacgccagaa acagtcccag cgctgaaaga gcctgaatat
    11821 tcgcaacagg cacctgacaa ttcccccgtt tcgatatgat ttcttttcga ttctgtattg
    11881 taacagtaaa aacgcagaag gggcttggtt cgaatttttc aatccatcaa gccgttgtgc
    11941 gcgcaaaaaa aaggccgcct tataaggcgg ccttctggtc aataacacat tcaggcaagg
    12001 ccgatctttt cccctgctga tctctgcggc gtctgtatct gtttgaggat ggcaatagca
    12061 acctcaagag ccctcttgcc atccattatc cctacaaggg gctgtctgcc ttccctgaca
    12121 caggtgacaa aatgctggag ttcaagtttc agaggttccc tcttggggaa aactggatgt
    12181 tcgataacct ctacaaggcc acattccttt tcctgtatac atctatgtat agaaacgtcc
    12241 tgagtttcgt aattgattgt aacaaatcgc tccggctcca taatttcaag ttgcctgagg
    12301 cgcctctctg aaacacggct gacaagaatc tgtgcgaggg ccccgttctc gaatgaaatc
    12361 tgcgcggatg caatgtcctc gaaagaagat ctgatggacc tgcccatcgc ggaaatattc
    12421 gagatctccg agtgaacaag agaaagaata atatcaatat catggatcat aagatcgaga
    12481 acaacgccca catcgttgat gcgggaactg aaaggcccga tccttctgga ctgaaggaaa
    12541 agtggcgcct tgataatgtt ccttacatgc tgaaccgcac tgttgaaacg ctcaatgtgc
    12601 cctacctgaa ggacaaggtt atgtgttgca gcaagtttca aaagttcttc tgcctcttct
    12661 accgtagagg tcacaggttt ctcgataaga acatgaacgc cggaggaaag ggcctttttg
    12721 gcggtttcgt aatgatagac cgtggggacc acaacgctga cggcatccgg cctggcctgg
    12781 ctgtaaaact tctctatatc cgaataataa ggtacacgaa gaagttcccc caccgctgca
    12841 gccctatcag ggttactgtc aacaatcccg actacatccg tattgagaag ttccgtataa
    12901 actcttgcgt gatgataccc gagatgacca accccgataa ccccgacacg aaccgggtcc
    12961 attaatatcc ctcccgcgat aacgattgcg gaaatatgat aaccccgctt taaacatttt
    13021 cccaccagaa aggttgtttg tggtaactgg aatacaatct cgccagaagg agagatcttg
    13081 cattcctgtc accctttctg taggaaaaac aaagagggtg agaacatgat gtacatattc
    13141 cggtatcggt tatattgttt atgggtattc ccatgtcata taattgtgta ataatagcat
    13201 tacggagatc aaaataaacc ctgccattct caactctgaa ggagtcaccg ggaaggcacg
    13261 ccatggctct catggtcgag ggatcttcca gacttctgct atagcagcat cctcctatgc
    13321 caggtccgat ccaggcatga cactcttcgg ccctggccat tcccacggtg gataacattt
    13381 tccgaagtcc ttttgaaacc gcacccctgc atgttccggc aaaaccggaa tgaacaagga
    13441 gacaccagtc aaaaggtttt tcagaaacaa ctatcaccgg gaagcagtcg gcgaacctga
    13501 gactgcccca tataccactg cgggaaataa ataccgaatc cgcctccggt ctggccggca
    13561 gggaccaaat agggaccgcg tctatcacgg cggtcccgtg tctttgcagg ggggcaacaa
    13621 acgtcgtctt tacagggtct tcaaacaaaa gggatgccgc cttttcggga gagccttcag
    13681 ccccctctgt aacggggccc ctgaggaaaa gtgtaacaga cattcttttg ctcatgtccc
    13741 ctggagggat gaaacggatc aacgactgtt tgccactgac agcaagtttc catttttcat
    13801 gttcgagggt cacgagaaag cccccttcat ctcatccgcg gatttgtttt ttgacttctc
    13861 caacaaggaa gagactaccg cagcagagta tccttccggc aaatccatat gctttttcaa
    13921 tggctttcat gggttcctcc tcaacgtcaa gaaaggtttc tgtattcatt gtcctggcca
    13981 tatcaagaag attccagggg tttgcggacc gcgctgtacc cgggactgag gtaaaaacta
    14041 cgaaggggaa gttctctaca attttcctga tcattccctg cagatccttg tctttcatgg
    14101 ccgcgaagac cacggcgcaa ttattggagt aaccgcaaag tcgaagagtc tctgttgtcc
    14161 cagcaacacc ttcaaggtta tgtcccccgt ccagaataag ttctctcccc cgaaaggaga
    14221 ctttttccag tctgccgggc caatcagttg aggaaagacc gctttttaca gttgacaggt
    14281 taattccgga atatttatca gccaaaccca gggccgccag aatggccgtc cttgcatttt
    14341 ccaattgaaa tattccaccc agtgatgtcg taaatcttcc cagttccata ttccctgttg
    14401 ccaaaaggaa agagttcctg tctactccag tctcagggtc cttgatacag gcctctcttg
    14461 aagcaacaag acccttgttg gagatccttt cgcaaagctg gtcatatctg gactccagtt
    14521 cactgtttcc gccagagaaa acgctgatcc ctcccggcct gagaaccaga aatttttcca
    14581 ttgctatggc ccccagatcg gggcccagaa attccgagtg gtccatcccg atggtggcaa
    14641 taactgacat aacgacacct ttcaaaatgt ttgtagcatc gaggcgccca cccataccgg
    14701 cctcaacgat ggcaatatca ggagcatatc ttgctataga atgaaaagca gctgctgtaa
    14761 gaacctcgaa ataggtcggt ctgttcttct ggtcaaaatt ggatctgaca gcagaacaaa
    14821 cttccttgac cgaatcaaga aaaatctctt tcgaggacgg aattccacaa aagcggatcc
    14881 tctcggtaat atccaccagg tgcgggctgg tgtacagaca ggtcctgtaa cccgctttca
    14941 gaaatacgga ttccagcatc gccgatatcg aacctttgcc gttagtgcct gcaacatgaa
    15001 ctgcctggaa attcctctcg ggatttccaa gtgcagacat aaggcgggcc atcctcgaaa
    15061 gccccgggca tatcagcgga ctggaaacat tttgaagcaa attttcgatt tcgttaagat
    15121 tatctctcaa tactcatatt ccccgtcagt tctactgcct cgacaggctt tcgatattcc
    15181 tttcgataag gaggatcttc tcctccgaag cggagaccct ggctttttcc ttttctacaa
    15241 tctcagccgg cgccctcgaa ataaaggaac tgtccgaaag ttttgctgtt gatctttcga
    15301 ggtctttttt agcttttgca agctcgccct cgagtcgtgt tacctccgat actgcatcaa
    15361 gcagatcacc ggcaaccagg aaaacttccc cgaaaccgca aacggccatg agagatctgg
    15421 gtggtcttgt cgatcccggc gggttaacac gaagaaccgc ggttcttacc aggagacaga
    15481 acaggtctgc gttgttgccc agaacttcag cagaaacccc ggctttatcg gagaggttga
    15541 tcacaacttc cctgatctcc tgctgcgggt gcatccgtgc ttctgccctg agatttcttg
    15601 catttcttac cgcttcctgg aatatctcca tctcgtcgac agaactggat cctccgggga
    15661 catgtttcgg tacaggccat tcttctttct cgataaaatc attgccaaat ccaaatttct
    15721 cccaaagttc ctctgtaagg aatggtataa aggggtgaag cagcctgaaa atattcgaaa
    15781 aaaccagttc gagaacggcc cttgcagcca tgccccgatc ttccccttcg tctcccctga
    15841 gggatggctt cgccatctca agataccagt cgcaaatctc gttccagacg aactgataaa
    15901 gggaacgggc ggcctcacca taaaaatacc cgtccagatc cctggtgaca ttttccgtca
    15961 ccaccgcaag cctttcgagg atccagcggt catgagttct caaagccgtt ccttccttga
    16021 aaggatcgca cttttctcca tcgaggttgg cgagggcgaa tttgctggca ttccatatct
    16081 tgttcatgaa aagcctgtaa acctctattc tctctggacc aaggaatata tcccgccctt
    16141 gaaccgtaag agccgcaagt gtcatcctta atgagtcgga tccggatttt tctataatct
    16201 caaggggatc tataacatta cccctggatt tgctcatctt ctgaccatgt tcatctctca
    16261 ctagggcatg aatatagaca tcagtgaaag gctccttgtc cataaagcga agccccatca
    16321 tgatcattcg ggcgacccag aaaaagagaa tgtcgaaacc ggttacgaga agactcgtag
    16381 ggtagaaagt ttcaagttct tttgtcttgt ccggccaacc cagtgtcgaa aaaggccaca
    16441 gggcactgct gaaccatgta tcgagtacgt cttcgtcctg gcatattctg gagcttccgc
    16501 aggcttcgca ggctgtcggg tcttcctcgg aaacagtcag atggccgcag tctgagcaag
    16561 tccaggctgg aatacggtgt ccccaccaca attgtcttga aatacaccag tcacgaatgt
    16621 tttccatcca catattatat gtactctccc actgggaggg cacaatgttg atccttccgt
    16681 cacgaacggc ctcgatcccc ggctttgcga ggggctcagt tctgacgaac cattgttccg
    16741 aaagataggg ctcgacgatg caatcacaac gatagcaatg tccgattgca ttatcatgtt
    16801 cctctatctt ttcaagaaga tcttccttct cgagaacatt taccattctg gttcttgcct
    16861 caaaacgatc cagtccggta aaaggtgctc cggcctcttc attcatgacc ccatgctcat
    16921 cgataacctg gatcgcttca agcccgtgcc tgttgccaac cagaaaatcg tttgggtcat
    16981 gggccggtgt tatcttgaca agcccagtac caaaggcagg gtcgaccatg ttatcctcga
    17041 taaccgggat tactctcccg gtaacaggga ctttcacaaa ttttcctgcc agagacgagt
    17101 tcttttcgtc acgggggtga accgctatag caacgtcacc gagtatcgtc tccggtctgg
    17161 tggtcgccac aacaagatat ccttcctctc cttcaagggg atatctcaca tggaacagtt
    17221 ttcccttttc ctcgtgatgc tcgacctcaa gatcagaaag ggcagtctgg caccggggac
    17281 accagttaat tatgtacttg cccctgtaga taagtccttc cttgtagagc cttacaaaaa
    17341 cagatctgac ggcccgggaa aggccatcat ccattgtgaa tcgttctctt tcccagtcgc
    17401 acgaggctcc aagacgcttg agctggttta tgatcctgct tccgtactcc ttcttccact
    17461 cccagatctt ttcgacaaag gcatctctcc caagatcatg cctggagatc cctttgcctg
    17521 agaggttctt ttccacaaca ttctgggtgg cgatccccgc atggtcggtt cccggaagcc
    17581 agaggacgtt tttgcctttc attctcatgt acctgcagga aatatcctgc agggtattgt
    17641 tcagtgcgtg tcccatgtgc agtgaccctg tgacattggg aggagggatg acgatacagt
    17701 atgagggttt ttgactctcc gcgtctccac gaaaaagtcc gccttcagtc cagcgtccgt
    17761 accacttttc ctcgatgggt ccggggtcgt atgatttacc gagagtcttt ccgtcagttg
    17821 ccatgataaa ataacgcctc cttggctgcc tgtgataccg atttaataat ttcaatcaaa
    17881 attatggatg accgaggcta ttctattcca gcgagtccag gtatgattcg agaaaaagat
    17941 ggaactcgcc ggttccccaa cccttctcca tcgaaacaag aataggttcg gatacggaag
    18001 aaaaagactt cgcagaatac cgggctttca gctgagccca gttgcccctg gataatttgt
    18061 cgatttttgt atataccacc tgaacaggaa cacctgtctt ctttatccac tcctggaaca
    18121 ggatatcgtt atccagcatt ccgtgcctga aatccaccag atgaaccaac aggaccaggg
    18181 attctcgccc ttgcacgtac tctccgataa gatcacccca ggcttttctt tctgccttcc
    18241 cacgcctggc aaaaccaaaa cccggaagat ccacaagagt aaaacctttt ttccgttcca
    18301 ccctgtaaaa gtttatgctt ctggtcttgc ccggggtcga actgacatgg gcaagccgag
    18361 tctggattat cccatttata aaggtcgatt ttcctacgtt cgaccttccc gcaaaagcaa
    18421 tttccggcag gaagggttcc ggaaactgct tgctgttaaa ggatgtagcc attatctcag
    18481 ctttatactt tccctggggc atccgttcca ccatcatcca gggcacttcg gaaaacatca
    18541 tccagtttat cgacaaacaa caggttcatt cccttgagca cccatcccgg catctccttt
    18601 gtttccattc tgttatccgc tggaagaatc acgttgaaaa caccatacct tttggcagca
    18661 agaatctttt ccctgatgcc accgacaggc aggacttctc cgcgaagtgt cacttcaccg
    18721 gtcatggcaa gtccgcttct aacgggtatt ccggaaaggg cagaataaat tgaaacagca
    18781 agggtaatac ccgctgatgg tccatctttg ggtatggccc cctcaggcac atgaacgtgt
    18841 atgtcggtct tgtcccaatc gatggaagag atacaaagat caccggcgtg ggccttcagg
    18901 taacccaggg ctgtctggcc ggattcctgc attatatcgc cgaggttgcc tgtaagaagg
    18961 attttcccct tgccagttat cattgcgctt tcgataacaa ggatatctcc accggtctcc
    19021 gtccaggcca gcccgatagc ggcacctctc tgactggaag aaggaagatg cactccgcca
    19081 aggtgcctgg gaactcccag aagggaaaca aggctggata ccccgacgct gaagcttttt
    19141 ttgctatttt tgtttttctt ttcttcggat tcgacaatct ttcttgtgat cttgcgggcg
    19201 atcctggaaa tttttctttc cagttccctt acccctgcct cacgagtata gtcggagatt
    19261 atccgggcca gcgaggagtt tgtgaatgat atctgttcgc ctttaaggcc atgttcctga
    19321 agaactttgg gaataaggtg ccttcgggcg atctggatct tctcttcgtt aacgtaacca
    19381 gggagcctga tgacctccat cctgtccaga agcggtttgg gaatggtgtg ggtgctgttg
    19441 gccgtggtta tgaacagaac actgccaaga tcgtagggaa cttccagaaa gtggtcagta
    19501 aatgcgttat tctgttccgg gtccaggacc tcaaggagag cagaggccgg gtctcccctg
    19561 aaatcaaggc ctatcttgtc gatctcgtcg agaagcatga ccggattcga tgtaccggcc
    19621 tgcctcattt tctggattat cctgcccgga agggctccga tatatgtcct tctgtgtcct
    19681 ctgatctcag cctcgtccct tattccacca agggacatgt taacgaattt tctgcccgtc
    19741 gcccttgcta tggagcgccc gagagaagtc tttccgactc ccggaggacc tgtaaagcag
    19801 agaacctgtc ccttcagcct gcttccggca tttttcctga cagcaaggta ctcgagaatt
    19861 ctttcctttg catcttccag agcgtagtga tcctcatcaa gaacttttga tgaaagagtc
    19921 acatccacac tgtccctcgt acttttcctc catggaaggc tcgtcatcca gtccagatag
    19981 gatcggacca cggtagcctc ggcggacatg gggggcattt tacccagacg gtcaagttca
    20041 tgaagggcct tctttcgaac atcctcgggc atccttgaag tgtttatctg ctctcggaat
    20101 gcctccattt cggaaaaatc ttctccctga ccaagttcat cctgaataac cttcagctgt
    20161 tctctcaggt aatattcttt ctgccccttc tccatttctt gcctgaccct gtcatgtatg
    20221 gatcgttcca tctccagcag ctcaacttcg cggaggagga tcttgagtat catctccatc
    20281 ctctggggta tatcagaagc ttcaagtatc tgttgccttt cagctatttt tacggtgaga
    20341 tgagaagcta caagatccga tatctgacca gagtcctcca gggaagaaac ggacatcatg
    20401 acttcaagag gaatcttggg atgaagtgta acgtatctct caaactgggc gaggagggtc
    20461 cttcctagag cctccgtttc tggcccggct ggaattgatg atccttcaag gggttctatc
    20521 tcggcgaaaa aggtatcatt ctccagagcc actgaattta ttgcagcacg tgatatgcct
    20581 tccacaagga cttttgtagt accgtcaggc agacgaagca tctggattat ggttgtaatc
    20641 gtcccggcac cgtaaatatc ctcgggctcc ggatcctcga ctgacatttc cttctgtgct
    20701 accacaagaa gggtcttttc atgcagcatt gcctcttcca gagccttgag cgaccggggt
    20761 cttccaacaa aaaggggaac tatgacaccc gggaacagaa caaggtcacg aacaggaaga
    20821 aggggcaaca tttttctttc ggagttttct tccagaacca actaggcgac ctccccatct
    20881 ctcgcataat ccagaaccgg aggttccttc ccttcgatca ttccgtcggt cacaacaact
    20941 tttgaaagtc cgtcgttcct cgaggggagt tcatacatca gatcaagcat ggccgactcc
    21001 ataattgacc gcaaggccct cgcgccggtg ttcctttcgc aagctttcct ggctatggcc
    21061 gccagagcct ccgccgtgaa ttcaagtgta acgttctcca tcctgaatgc tttctgatac
    21121 tgccggacaa gagcgttttt gggttcggtc agtattcgta caagggcctt ttcatcaaga
    21181 ccttcaaggg cagtcgttac agggaccctc ccgacaaatt ccggtatgaa accgaaagac
    21241 ataaggtcat caggctgtac gtccttcagc accctgaaac ggtttttttt ctcgtttctg
    21301 gacacctggc ctccaaagcc tatgatcttt ctgttcgtcc ttcgggaaat aatgttctcc
    21361 agaccatcga aagcacctcc acagataaag aggatatttt tggtattgat ctggatgaac
    21421 tcctggtggg gatgttttct cccgcccttg ggaggaatgt ttgcctcagt accctccagg
    21481 atcctcaaaa gggcctgttg taccccttct ccggaaacat cccttgtaat tgatgccgat
    21541 tcggatttcc tgcagatctt gtcaatttcg tcaagataga tgatccctct ctcagctgca
    21601 gcaatatcgt aatctgcggc ctggaggagc ctgacaagaa tattctccac gtcctcccca
    21661 acataacccg cctctgtaag ggtcgtggca tccgccatgg caaaggggac cctgatcgtc
    21721 ctggcaaggg tttcggcaat gagagtctta cctgatccgg ttggccctat aaggaggaca
    21781 ttacttttct gcatctcaag atcgtcttca ataccacagg ctattctctt gtaatgattg
    21841 tagaccgcta ccgaaacaac cttcttggcc tgctcctgat cgatcacata ctgatcgagt
    21901 tgttccttga tctccaccgg tttgggaaga tccctgaatt ctttctcaaa tggcacggga
    21961 agagattttc catcttcgtt ctctctgaga ataaggttgc aaaggttgac gcactcgttg
    22021 catatatata caccaggtcc ggcgatgagt tttgtcacct catcctggct tttaccgcaa
    22081 aatgagcacc tggcttcacg ttttgtcttt cttgatccgt ttccggttgt ccctgacaat
    22141 tgtttccccc ccttgttaca gatggttccg gatttttcca ataattcagg gccatattac
    22201 ttcattttta cccctgggtg aacaaaaagg gagcgcctgt aggagctccc ttttccgggc
    22261 gcatatggta ttttatccct tgaagggatt ttacctttgg gcgatcactt tatcaataat
    22321 cccgtacgac acagcctctt cggcagacat aaagaaatcc ctgtctgtat cagcactgat
    22381 ctcgctctct gtcttgccgg tgtgagcgac aagtatttca tggatacgat ccttaagcct
    22441 cattatctcc cttgcatgta tctgtatgtc cactgcctgg ccctgggttc caccgagggg
    22501 ctgatgaagc atgatcctcg agctgggaag ggcgatcctt ttccccttgt ccccgcctgc
    22561 cagaagaacg gcggccatgc ttgccgcctg accaacgcaa atcgtggaaa tgggacactt
    22621 gatgtatcgc atggtatcgt agatagcaag tcccgaggtg ataacgcccc cagggctgtt
    22681 gatatagaca ttgacgtcct tgtccggatc ctcgctttca aggaaaagaa gctgagcgac
    22741 aacaaggttg gacaaatgat cgtctatggc gtcgccaatg aatatgatcc ggtctttcaa
    22801 aagtcgacta tagatatcgt aggctctttc tccccggcct gtctgttcaa taaccatcgg
    22861 gataaacatc aggcttttgc ctccttctgg tcaaccatga tctcttcctc ctggatctct
    22921 gccttttcaa gtatcctcgc cacagtcttg cggatcctta ctttgtggat cagttcagaa
    22981 agatccttgt tttttttgag ataaaattgc cgcatctgat ccacgtcgac cctgaaagcc
    23041 tccgccatat tcgcaatttc agtttcgata tcaccttttt cgataatgat attttcctta
    23101 tcggcgacag cctccataac aaggcttctt ttcacaatgt tcgcggcgct ttcctcggca
    23161 tctttttcga taaattccat ttcaagaccg ttggccgaaa tgtactcctc cagttccttt
    23221 ccagtcttgt ttttgatgtt atccctgatc tgtttaaggt tttcctcttt ctgtattttc
    23281 actagacttt caggaacatc aacctcggag ctcgcggcta ccgcctcaat ggcattgttc
    23341 tccgtcaatt ccctgcaatc acgatcaaaa ttatcctgaa tacctttggc taccctttcc
    23401 ctgaagacct cggcggtgat atctttctcg ccggtgatct tctcgaaaaa ttcagcccca
    23461 agttcgggaa ggaccttttc ggaaaccgaa agaatcttca tccggtaaag ggcctttttt
    23521 ccctcttctt caccctctgc cttgagcatt acttctgcct caacctcatc accagcattt
    23581 tttccttcaa gggcattaga aatcgcgggg tccaatgaat cagaacctat ctccaccgca
    23641 cctttctggt gctttctatc accttcatcg aggcttttcg tgtttttctc aagaactacg
    23701 ctgtattcca gttccacaat atcgccatta tgggatgacc tgtctgatac aggcaaggtt
    23761 gaagcatgtc tctctctcat acgttcgatc gcctcatcaa ccatggtatc agtaaccttg
    23821 aaggttttgg caatcacctt gacagtgtcc agttcaggaa ttgtgacctc gggccttacc
    23881 tcgaatgttg ccgtgatcct gagagggctt tgagcctcta ccgtgtctat ctcaagggat
    23941 ggatcagcga tcaggtcaag ttcgtattcc ttgacaactg agtcgagata ttccggaagc
    24001 attttctcta ttgcctcggt tcttatgtaa tccattccta gatgcagttc aaggaccttt
    24061 ctgggaacct tccctttcct gaaccccttt atgttggctt tcgccctgag ttcttctgtg
    24121 gtctcttcta ccctcttagc cagtgtctct tcctgaacct caataaggat tttaaccttg
    24181 ttcttttcct gggagattat ctccgatctc aaattaggac agccccttcc gattatcctt
    24241 cttgttaaga tatttctttt tccactaaaa aggagaaccc cctggcgact ccaaggggtt
    24301 cccttcgaga aatgcttctt taaagacgcc ttgaatgctg gtgcgagaga ggggactcga
    24361 acccctatgc cgtaaggcac tggatcctaa gtccagcgtg tatgccaatt ccaccactct
    24421 cgcagtaaaa tggtgggtca tacaggaatc gaacctgtaa ccccctgatt aagagtcagg
    24481 tgctc
//
LOCUS       DAOTIQ010000022        23996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2468_length_23996_cov_9.034961, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000022 DAOTIQ010000000
VERSION     DAOTIQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 23996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23996
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_23996_cov_9.034961"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(630..1796)
                     /locus_tag="P5541_04460"
     CDS             complement(630..1796)
                     /locus_tag="P5541_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV71264.1"
                     /translation="MRIFSFISFLVIVLSLYGALNLYIWLHLARVVPHLGLLKIPVVT
                     AFLLLVAAFPIGRIGGAFCRCSFTDILAVVGSWYLGFMIYVMILLALFDIILLADNWL
                     RILPRSLSGKGGKTVIAAFWIVITISGLIVAAGRWNAVNPVIREYDVTLEGTPGEAPL
                     RLVVATDLHVGLMVRNHWLNEMIDLINAASPDVVLLVGDIVDSDVTLAQEEDLSGVLA
                     EISAPLGVFAVLGNHEFYSGPEEAEKSFEDGSVTVLRDESVVVGGVFNLVGRDDRASS
                     RLGRERLPLKDIVLADKDLPVIVMDHTPSDLHEAEEAGVSLQVSGHTHRGQLWPFNYV
                     TSRIFEQDWGFLQKGETLIYVSCGLGVWGPPIRTSSRPEVVVLKVSFKGSVNSE"
     gene            complement(1968..4328)
                     /locus_tag="P5541_04465"
     CDS             complement(1968..4328)
                     /locus_tag="P5541_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HRV71265.1"
                     /translation="MNERIERLRTESFEAPVTLDHERAGIVTDFYIENFGKNSVPVTR
                     ALAFKALCEKKSIYIGKDELVVGERGPFPKSVSTYPELNCHSLEDLEILDTREKNPYL
                     VPQKCREVYRDKVIPYWRGRTMRDKIFADIGEEWKETYAAGYFTEFMEQRAPGHTTLD
                     GKFYSKGMEDFKKEIAEAIACLDFKIDPEATKKREQLKAMDITCDAVIIFAERHAELA
                     KKMAAGEGDPERRKELEKIASNCMNVPRRAPRDFWEALQMYWFMHLGTITELNGWDAM
                     NPGHLDRYFDPFYQEEIKAGTITPEWAKELLSAFWIKFNNHPAPPKVGVTAAESGTYN
                     DFTNINLGGLLPDGTDGVNDISYMMLEVMDEIHLLQPQANIQLSRKNPDRFLKAALRV
                     IRKGYGYPSVFNADTVIEQMIRAGKTVEDAREGGTSGCVETGAFGKEAYILTGYLNTP
                     KIFELALNNGVDPVSGKKIGVETGDPAGFKSFEDLYAAFEKQLHHIVDIKVRGNQFIE
                     RMYADYAPAVFLSVLTDDCIKNGKDYNDGGPRYNTNYIQCVGIGTMTDSLSALKKHVF
                     EEKTFEMSEVVASVGKNFDGSEAMRLTFANKTSRWGNDDDYADDLMKRVFNSLFDEID
                     GRRNTKGGEYHIDMLPTTCHVYFGSITGATPDGRLAGTPLSEGISPVQGADRNGPTAV
                     IKSCGKMDQIRTGGTLLNMKFMPKVLEGEEGIDKLAKLVRTYFRYDAHHIQFNVVDTE
                     TLRDAQEHPENYRDLIVRVAGYSDYFCDIGPDLQEEIISRTAQEEV"
     gene            complement(4334..5248)
                     /locus_tag="P5541_04470"
     CDS             complement(4334..5248)
                     /locus_tag="P5541_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRV71266.1"
                     /translation="MNGNTRGIIFDIKRYSIHDGPGIRTTIFLKGCPLSCWWCHNPES
                     QSAFAEISYRGDRCIGCGACVTACPAGAISLTQRGYVADPDRCAGCGECVRACPAEAR
                     EMIGREVGVEEILKEIEKDLLFFDESGGGVTFSGGEPLSQPVFLVEVLRECCRRDIHT
                     VVDTCGFATLDVMKQVAPYTRLFLFDLKMMDTSLHRKYTGVPNEVIIENFRWLMSEKH
                     NVRVRIPVIPGINDSLENMEATGEFLSGFPSRPEVNLLPYHGSATEKYHRFSIPYRMA
                     DVSAPSGERMKELSDQLARFGLAVSTGG"
     gene            complement(5245..5961)
                     /locus_tag="P5541_04475"
     CDS             complement(5245..5961)
                     /locus_tag="P5541_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71267.1"
                     /translation="MTPDCPFNMVSLRQQVYDYLRSQMNQGELRPGSAINLGEMSSRF
                     GISRTPLREALLQLETEGFVTIYPRRGIIVNPLSEEDIRHAYEIIGALEASVVFNERD
                     RITLTETEEMLRLNLLMREALDRDDFDTFYRHNISFHNIYLDLSRNQSLVRTVMTLKQ
                     RLYDFPRRKGFVKEWEITSTGEHERFIELLETGDAKAASDYMRDVHWSFEVQKPWIRR
                     YYSELEKERNLEGIRAEVIR"
     gene            6107..7660
                     /locus_tag="P5541_04480"
     CDS             6107..7660
                     /locus_tag="P5541_04480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRV71268.1"
                     /translation="MKRVSILNHVIGPIMRGPSSSHTAGPWRIGRTARDLLGSRPKEA
                     IFTFDPKSSLAVCFHDQGSDAALVAGLLGFTLTDDDEPRILEMALEEGLDATFRLAPF
                     PEADHPNSTLLHLKGEEGEELTLHARSVGGGSMQIASINGFPVMIKGDRYELAVECEA
                     SSTEKVISLLKTWDPDAAAQTDKGRGFAHGSSFLKPEETLLSSIKSLTGVGGFYKADP
                     VMHPLRGKELFPDTGGLLALAKEKGWSLGEAALEYESALLELPREQLMEEMGIRLDIM
                     LRAVELGLKGDHLEMKLLRPFAGRVMKAEEAGKMFLGGPHARAAIRAMAAMHINGAGG
                     VVCAAPTGGSAGVMPGVVSTLLQDMEVTREKVIMAMWAAGAIGLALDRRSTFAAEVAG
                     CQVEIGAAGAMSAAAVVETAGGSADQGCAAAATVFQNMMGSVCDLIQGIVEIPCHSRN
                     AALASQAFLCADLVLGGYENPVPLDETVDAVDSVGRMLPEELRCTSRGGLAVCPSALG
                     LCRLDRCTK"
     gene            7690..8682
                     /locus_tag="P5541_04485"
     CDS             7690..8682
                     /locus_tag="P5541_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRV71269.1"
                     /translation="MLVISGEEIRTIYPMKDAIEATKHAYSLFSEGKSDVPLRTSLKV
                     KEHNGNVLFMPAYISGENPLVGIKIVSVYVDNPGKGLPAIPSTVLLVDAENGFVKAIM
                     NGTVLTQIRTGAASGAATDLLARPDSKIGALFGAGGQAEFQLEAMITARTLEEVRVFD
                     PRIENCRSFIERIKDKEPFSGVRLVVAETSDEAVRDADIITAATTAKRPVFNAGSVKP
                     GVHINGVGSYTHEMQELDPGILNRAGKIYMDSIEAVLAESADFINPLNEKTFSRDSIT
                     GELGELAQGKKPGRASSEEITLFKTVGIAVQDVVAAEEIFKRAVIHKAGKTISF"
     gene            complement(8794..9630)
                     /locus_tag="P5541_04490"
     CDS             complement(8794..9630)
                     /locus_tag="P5541_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV71270.1"
                     /translation="MNSTEKVTWILKRLGEPPHEMGISQIADEMGFSRSGIHKILSTL
                     VREKFVSQDTRSRKYMLGPALSRIAIAYRGDRSIIDVADPVMKDLLEKTGESVGLCVK
                     EDGIPILVHKVVSNQPLRLDNQVGTVIKLNRGALGKVIAAFEAEEKIEALLQEPLEKW
                     TEQTILDPLKLREEYRRIREKGYSISAEEVIPGILGIAAPIFDSRGECRAAITVGGPT
                     VRLTLQIMEGFAPMVLNAASLIAENLGEKSQPISPPREAACNSSEAWNKCQKNKGEEG
                     RQ"
     gene            9784..10968
                     /locus_tag="P5541_04495"
     CDS             9784..10968
                     /locus_tag="P5541_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV71271.1"
                     /translation="MPEREAFLKAAENARERIIGWRRDFHSHPELAYQEVRTAGIVAN
                     VLDSLGYTVETGIGGTGVVAKIKGVLPGTSRGLRADMDALPIAEETGLPFRSGNEGIM
                     HACCHDGHVAMLLGAAEMIAQHKEQLKGEIVLVFQPAEEGYAGAQKMIDDGLFKKFPV
                     DFLFGHHIMPVILPHNSITTRKGAFTASSDRFHFRIKGYGGHASMPHLTKDPVPALQS
                     LIGGINSITSRNTDPFDQAVISIGHIGAGTCYNVIPDSAFMSGSVRTLSEQSQQLVQK
                     RMTQLAKGISEAHEVEISMEYKVNYPTLFNDATLTGRTLELARTFWGEEKVIEKQDPF
                     MGSEDFAFYSKSVPCCFLMIGVEGEFGVHHPKMTFNESILHKGAAWQAWLALESGEIS
                     LL"
     gene            10994..12340
                     /locus_tag="P5541_04500"
     CDS             10994..12340
                     /locus_tag="P5541_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71272.1"
                     /translation="MQKVLEQFIFLSIFLLAGYGVRVWFKPVQKLFLPASLIGGFIGL
                     ILCPNGFGIIPVTPEMMKELGALPGILITVVITASVLGVKLPKPGELAGGLARNFMHL
                     NVFFMTQIGVGLLVGAVFMNSTYKTFGIELVAGFAGGHGTAAMLGSSLKDMGLDWWLN
                     SQGVGLTTATVGIIGGILIGIGLVTYASKKGFVSGFKGIDQLPKEMIIGYSKPEDAKP
                     MGRQIQYPAAIEPFAFILSLVLISVGLAFKFRVMLTGTVMFRVAPWAWGIIIMAIIWM
                     LICRLRLDWLIDPVAKARLMGTFVDYLVVAAIISLPVRAVWQFIVPITVLCAVGLAVT
                     IFVTMFLGRKMLPEKDWFERSIISFGQCTGVGATGILLLRLVDPDFQTEALSHWSLAF
                     SVISLYFWFLFASLPILVMNHGLLLTGLFFTGLSLAIVVASRVIPGFWNTDTATGS"
     gene            complement(12375..13157)
                     /locus_tag="P5541_04505"
     CDS             complement(12375..13157)
                     /locus_tag="P5541_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV71273.1"
                     /translation="MNSFEKMAWLLKRLGEPPFEMGLSEMAGEMGTGRSGIFKIMMVL
                     LRENFVVQDPLTKKYSLGPVLFRLGNTYKQEKSILEVAEPFLRDLASRSGESVGLCIR
                     EGDIAVLAHQIVSDQPLRYEGKIGSRLGPNRGAQGKLMAAYEPEERIREDLFSGPLES
                     RTPKTITDPDVLLQEYAMIRKQGFATSDEESFEGIRAIAAPVRNRHGEVWASLAVAGP
                     TVRLTNIRLNQLIPFVVETADRISSGFGFRKGSGDHNDKGAG"
     gene            13283..14386
                     /locus_tag="P5541_04510"
     CDS             13283..14386
                     /locus_tag="P5541_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRV71274.1"
                     /translation="MSDCRIRSISTTVLEMPFRKTWQISLYSAATRPHLFVRVETEDG
                     IYGYGEASPSPAFMGETAYTAREVIDRHLKVALIGVSIHDRSTIHTRMDRILHGNSGA
                     KTAIDLAVHDSAGKALGVPVCRLIGGKLRENVPQAWVLGIQEVKDGLEEARFMLEKGY
                     GTIKVKVGTNPPREIELLKALRSEHGNDFKLRVDANQGFSVRETMAFLQKTSHLDLEC
                     IEQPVPRWDIKGLREIRSRGGVPVMADESCSSPQDAIRVIEAGAADILNIKVAKVGGL
                     YRAVQTASLAEAAGLSAVAGSNIEAGLGSWANIHFAASQACMCHDNDQIAVPLYENDI
                     IEEEFDLASGGIPVTDKPGLGVSLSKQIINELR"
     gene            14435..15514
                     /locus_tag="P5541_04515"
     CDS             14435..15514
                     /locus_tag="P5541_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HRV71275.1"
                     /translation="MLNNKNARRMTVLVIAFLISVFPVFSGMTLAEAKTITLSYAESG
                     PPSGLGGAFVNIMKEEIEKATDNQVTIEVFWQGSLLKGKEILEGVKNGMVDMGYVLPA
                     YFPKQLFVHNAFTLFPQGPTGFKNVVSAITRSFETLPEFGEELSKWDQRVLYMRFMLP
                     MALTSTRPVEKLEDFKGMKIRASSSAYLKALQGAGANPVSVPWGDCYMALQTGTVDGV
                     FTNFDGIHATKLHEPGRHIFTTRQLWVPMPIFVTINNKTWQKLPEDIRDKMLKAQEPI
                     LARFAELFEKEWDRIVEDQKKEGATIVRASDKDIEDFVSLQAWNDLRAEWSAEAPAMG
                     VKDPQHFLDTLGKIIGEEIDKEKKQ"
     gene            15558..16037
                     /locus_tag="P5541_04520"
     CDS             15558..16037
                     /locus_tag="P5541_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV71276.1"
                     /translation="MFKQIIHQINNALAELSGWLVTAIMALLILDIISRLSGRAVQGV
                     SEFAVFALVATVYLGLSHCEEKESHLRVEFFSDRFKGRSKRISDTFDYAVAVIFVSVA
                     LYASWRSALFSFQTGESVPGTIPLPIYPAKFVVFTGLLFYLAQLIINLGSKWTGGDN"
     gene            16049..17356
                     /locus_tag="P5541_04525"
     CDS             16049..17356
                     /locus_tag="P5541_04525"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV71277.1"
                     /translation="MIGLLGILILLALLGKGVHIAIDFLVVGFVGISALVGIDTAISF
                     LGQTLYHTIATPSYCALPLFTLMGAFASMGAFAEKAFEGIHDLTRKLPGSLAIASCFG
                     CAAFGAVSGSSLATAALFGKIVLPEMMRYRYNKPFAMGCIAAAGGFAAMIPPSAGFIV
                     YAIFTDQSIGKLFMAGILPGILTAVAFSVMIVIKVIRNPELAPREIERSHDKKTLLST
                     FGSIKKMWSIALIAALVLGGIYTGIFTPTEAGAVGALAALLIGFQQGKIRSISVIRKA
                     MRESAYTTSMIFIIMVGALFFGRFLALTRVPTWASMLITGWDVPRIVVMGGILLVWFL
                     LGMIIIPTGIMALTLPIFFPIIVSLGYDPIWFGVIIMKLGEIAAITPPVGLNVYAIKG
                     VAGKGTTLEDVFRGVWPFVICELVVLAILIAFPQISLWLPGMM"
     gene            17409..18314
                     /locus_tag="P5541_04530"
     CDS             17409..18314
                     /locus_tag="P5541_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV71278.1"
                     /translation="MKKVIALKVDVDTLKGYLEGVPAMLSLFERHGVRASFFFSFGPD
                     NSGKAIRRVFRKGFIQKMLRTNAPGTYGLKTMMYGTILPAPLIVPRKPDIFRRAFDQG
                     HECGIHAWDHVAWQDTLDRMPEKKMRDHFSRAFEMFSSIAGQRPRCCAAPAWKVTPAS
                     LTMQDEYRFDYCSDTRGVFPFMPIMEGTVFKTQQVPSTLPTMDEILGSEGTDVKNIND
                     HYMEAIKPGLNVHTIHAEMEGGVMIHLLEELVARCLNEGISFMTIGEAINGNDPADLP
                     LSPITNHEIPGRAGAVAVQSLQTKA"
     gene            18402..18872
                     /locus_tag="P5541_04535"
     CDS             18402..18872
                     /locus_tag="P5541_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRV71279.1"
                     /translation="MEKGSFDMKKALVYAIHAEIEASEFYSDWAKHAEGHLKKELEEL
                     SGWEGEHRDSLVKYLENSFGEKFSRDPEMVVDPALKVQADEFRNNYALLRIASTVFLS
                     EMRAMELYEKMESESTGEAKKMFREIKEMEKGHMDSAKKRYMELREHIVGFHAF"
     gene            complement(18949..20094)
                     /locus_tag="P5541_04540"
     CDS             complement(18949..20094)
                     /locus_tag="P5541_04540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV71280.1"
                     /translation="MGRNTADVAIIGGGVHGCSIAYHLAKKGVKAVLFERDYLSSGSS
                     GRSAAGLRYQFGTEVNCRLSMYNMEKIPHLQEELEYKADLEYDPAGYLWIAYSESQLE
                     QLKKNVALQNSLGIDSVILDPAGIKEVAPGLNVEGMLGASFNQKDGHVNPHTMNFAYG
                     DAARRLGAEIHTKTAVTGLLTDNEKVWGVKTDKGDWEAGAVVCAVNAWAPEVCSWCGL
                     KVPIIAERHQILITEPVERMKHPMTLCLDDGSYWKQCPNGTFMLGWGNPEEKKDTSYE
                     SSWPFLEEVSRRVIEKMPCLAGVRVVRQWTGPYAITPDEQAIMGKTPVENFYFDGGWS
                     GHGLQFAPSAGRIMSEMILGEEPFIDVKDFRFSRFEEGCLFREPACI"
     gene            20300..21307
                     /locus_tag="P5541_04545"
     CDS             20300..21307
                     /locus_tag="P5541_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRV71281.1"
                     /translation="MEITRATAVVDSHTGGEPTRIIIGGGPYLPGKTMSKRWAYLREN
                     LSEFRNFVMHEPRGHSDMFGAFLTPPEREDSHYGVIFMDSGDGVSMCGHGSIGTANAL
                     VELGMVPRTEPFTEVVLDTPAGQIRLKVEVKDGKVGNVTLANVPSFVYKSDVELAIPS
                     RNKKIHMDIAFGGNFFAIVHAEELGLTLEREEVPELLALGLEILRESNKQIKVQHPTE
                     KHLNYIGLTEFALERPGKPTKNCVIFGESNVDRSPCGTGTCAKMALLAAKGTLKPGEV
                     FDHESVVGSVFHGHYQPGPKVGDFETILPFIRGKASVTGFNFLLQQNNDEMGSGFLLP
                     K"
     gene            complement(21359..23155)
                     /locus_tag="P5541_04550"
     CDS             complement(21359..23155)
                     /locus_tag="P5541_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV71282.1"
                     /translation="MWIRSEYEQFSRQSDEIRRDYLQSQKVLLSSEVQRPVDHIRFMT
                     TRTESVLEENIRNRTEEALAIAWNIYEKFGHRLSVAETKRMIKEALRPIRYRDGKGYF
                     FIVSLDGVEELYPVFPDFEGKDVTDLRDERGNYVIREEIQVVKEHGEGFVTGYWKKPG
                     HENEEASPKISFVKLFEPFGWYIGTGEYLDDVQMAIQREALDFVSSLRFGPKGEKRLF
                     VRSFEGEDLLNDIQAGGEDVCLWGDDPAWREKVIREQIDAARQNPGGIFLEHHAPPSG
                     GCGSSDRLAFVIAVPEWGWVVGAGVMTDEIEGLIAAGGDRLKEEVLRRFLQMSVTVLF
                     LILIALVMLQFVAMRVRRQYSSFFSFFEKASVDYSEINPESFRYREFAELAAAANKMI
                     QERTRMENGIREMNDLLQGRVSDQSRKIEDLNQNIESRIEQRTKELKKLTETDGLTGL
                     FNRRYVFEMLELLVEGTSVAGKNLSVIMLDLDDFKKLNDTYGHVFGDRVLEAVARTIQ
                     GCARSSDIAGRYGGEEFLVILPETGLEAAAKVADRIRQGVSELKLDIPGSKVTISGGV
                     AEFDGDSPTSLVERADKLLYKAKRGGKNRIER"
     gene            23590..>23996
                     /locus_tag="P5541_04555"
     CDS             23590..>23996
                     /locus_tag="P5541_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HRV71283.1"
                     /translation="MKRRKLSVASVIAVAVLLAIAGGRIFIHTADVVIYSETESDRLL
                     GRLHQKQRGGVQVQGSGEVVRILPDDNKGSRHQRFILALSTGQTLLVAHNIDLAPRIE
                     DLRTGDKVEFMGEYEWNPAGGVIHWTHRDPSGK"
BASE COUNT         5744 a         6458 c         6185 g         5609 t
ORIGIN      
        1 cgtgaatcgg gagtcggaaa tcgtgaatcg aaaaaccatg gagacaggag gctggagact
       61 ggaggctgga agggcaaggg ccttaattcc aagtgcgtga acggtaaacg gtcagcggtg
      121 aacgggaaac aaagcagagg cgggaggcgg caaggccaag ggccggagtg aagtctttac
      181 cgaaggctca ggagccggtg agtttcgcag ccgtcgcgga ggggcaggaa aagcacaccg
      241 gccggagggt tcacctcggc actgtgcgat cgccccgaag caggcggaga aacgaacgct
      301 ttcaggcgag ttgcctttcg gaaaagactt cactacgcac tctcataaaa cgatcccccg
      361 gcccttctca ccacagggga cacagagggt ggttccacgc agagaagtgc aaaacctttt
      421 ctttgtaaga acttgggacc caaggttttg tcattctgag aaccagcggg acgaagaacc
      481 tggtttgtag tttttgaact attcgattca cgattcccga ttcacgattt acggtttttc
      541 cgtttactgt tgaccgttga ccgtttacgc cttttgcttt tgtttcgatt cacgattccc
      601 gattcacgat tcacgggttc ttctccaaac tattcgctat taaccgatcc tttaaaactt
      661 accttcagca caaccacctc cgggcggctg gaggtcctta ttggcgggcc ccacacccca
      721 agcccgcaag atacatagat cagggtttcc cccttctgca gaaagcccca atcctgttca
      781 aagatcctgg aagtaacata attgaagggc cacaactgac ccctgtgggt atggcccgag
      841 acctgaaggg atactcccgc ctcttctgcc tcatggaggt cagatggggt gtggtccata
      901 actattaccg gaagatcctt gtcggccagg acaatatcct tcaggggaag ccgttctcgc
      961 cccaatcttg aagaggccct gtcatccctg cctacaaggt tgaaaactcc gccaaccact
     1021 acggattcat cccgaagcac cgtaacgctc ccatcctcga aagacttttc cgcctcttcg
     1081 gggccggaat aaaactcatg gttaccaagg acagcaaaaa ctcccaaggg cgccgatatc
     1141 tcggcaagaa ctcccgaaag atcctcttcc tgggccagag tcacatcaga atccactata
     1201 tctccgacaa gaagaacaac atcaggcgaa gcagcgttga tgagatctat catttcgttt
     1261 agccagtgat tgcgtaccat gagaccgaca tgcaggtctg tagccactac aagccgcaag
     1321 ggagcctccc cgggggttcc ttcaagggta acgtcatact ccctgatcac cgggtttacc
     1381 gcgttccagc gccctgccgc aactatcagc ccggaaatgg ttatcacgat ccagaaggcc
     1441 gcaataactg tttttccacc cttgccggaa agtgaacggg gaaggatcct cagccagttg
     1501 tcggccagga ggattatgtc gaatagcgca agaagtatca tgacatagat catgaaaccc
     1561 aggtaccatg accccacaac agcaagtata tccgtgaagg agcagcggca gaaggcacca
     1621 ccgatccttc caatcgggaa ggccgctacg agaagcaaga aggctgttac tacggggatc
     1681 ttcagtaacc ccagatgcgg gaccaccctg gccaggtgaa gccagatgta gaggttgagg
     1741 gccccgtaca ggctcaggac tattaccagg aaggagataa aggaaaaaat tctcattgaa
     1801 cctccagggt agtcaaaagc gtgaacggtg aacagcctgt ttacaggttt tcccgttcac
     1861 gcttaccctt ttctggactt aaactttaaa atccaatttc caattcggtt cacgattcac
     1921 gatccacgat ttacgattta cgattcacga ttcacgattc acggttccta tacctcttcc
     1981 tgtgccgtac gggagattat ctcctcctgc agatccgggc cgatgtcgca gaagtagtcg
     2041 ctgtaacctg ccacgcggac tatcaggtct cggtaatttt ccgggtgctc ctgggcatcc
     2101 cgaagggtct cggtgtctac gacgttgaac tgtatgtggt gcgcgtcgta acggaagtag
     2161 gttcgtacaa gttttgcgag cttgtcgatc ccttcctccc cctcaaggac ctttggcatg
     2221 aacttcatgt tcagcaaggt tcctccggta cggatctggt ccatcttgcc acaggatttt
     2281 atgaccgccg tcggtccgtt ccgatccgct ccctgtacag gggatatccc ctctgaaagc
     2341 ggagtgcccg caagacgccc gtcgggggtg gccccggtta ttgatccaaa gtaaacatga
     2401 caggtagtgg gaagcatgtc aatatggtac tccccaccct tggtgttgcg tcttccgtct
     2461 atctcgtcga acagggagtt gaaaaccctc ttcatcaggt cgtcggcgta gtcgtcatcg
     2521 ttgccccacc tggatgtttt gttggcgaag gtcaacctca tggcctcgct gccatcaaag
     2581 tttttgccta ccgaagccac aacttcactc atttcgaagg ttttctcctc gaagacatgt
     2641 tttttcaggg cgctcagact gtccgtcatt gtccctatgc cgacacactg gatatagttg
     2701 gtgttatagc gcgggcctcc gtcgttatag tccttgccgt tctttatgca gtcgtcggta
     2761 agaaccgaga ggaaaaccgc cggggcgtag tctgcgtaca tgcgctctat gaactggttg
     2821 cccctgacct tgatatccac aatatggtgc agctgtttct cgaaagcagc gtaaagatcc
     2881 tcgaagcttt tgaacccggc cggatccccc gtctcgacgc cgatcttctt gcccgatacg
     2941 ggatcaacgc cgttgttgag agccagctca aaaatcttgg gggtattgag gtacccggtg
     3001 aggatgtagg cttccttgcc gaaggcaccc gtctcgacgc atccgctggt acccccctcg
     3061 cgggcgtcct cgacggtctt tccggcgcgg atcatctgct ctatgaccgt atcggcgttg
     3121 aacaccgacg ggtagccgta accctttctt attaccctga gggcagcctt gaggaacctg
     3181 tcgggattct tgcggctgag ctgtatgttg gcctggggct ggaggaggtg tatctcgtcc
     3241 ataacctcga gcatcatata ggaaatatca ttaaccccgt cggtgccgtc ggggaggagg
     3301 ccgcccaggt tgatattggt gaagtcattg taggtgccgc tctcggctgc ggtaactcca
     3361 accttggggg gcgccgggtg gttgttgaac ttgatccaga atgcgctgag cagttccttc
     3421 gcccattccg gcgtgatggt gccggccttg atctcctcct gatagaaggg gtcgaagtag
     3481 cggtcaaggt gtcccgggtt catggcatcc cagccgttaa gttccgtgat ggtccccaga
     3541 tgcatgaacc agtacatctg aagggcctcc cagaagtccc ggggagcacg gcgcggtaca
     3601 ttcatgcagt tggaggctat cttctcgagt tccttcctgc gttcaggatc gccttcaccg
     3661 gctgccatct tttttgcgag ttcggcatga cgctcggcaa aaatgatgac cgcgtcgcag
     3721 gtaatgtcca tggccttgag ctgttcgcgc ttttttgtcg cctcggggtc gatcttgaaa
     3781 tccaggcagg caatggcttc ggcaatttct ttcttgaaat cttccatgcc tttagagtag
     3841 aacttgccgt ccagggttgt atgccccggg gccctttgct ccatgaactc ggtgaaatag
     3901 ccggcagcgt aggtctcctt ccactcctcg ccaatgtcgg cgaatatctt gtcgcgcatg
     3961 gtgcggccgc gccagtaggg gataaccttg tcccggtaga cctccctgca cttctgcgga
     4021 accaggtagg ggttcttttc ccttgtgtcc agtatctcca ggtcctcaag ggaatgacag
     4081 ttgagttccg ggtaagtgga gacactcttg gggaagggcc ccctctcccc gactaccagc
     4141 tcatccttcc ctatgtatat gctcttcttt tcgcacaggg ccttgaaggc aagggcacgg
     4201 gtcacgggca ctgagttctt gccgaagttt tcgatataga aatcggtgac tatcccggcc
     4261 ctctcatggt caagagtaac gggcgcctcg aaactctcgg tacggagtct ctcgatcctc
     4321 tcattcattt ctctcatcct cctgtagaca cggcaagccc gaaccgggca agctggtcgg
     4381 aaagttcttt catccgttcc cccgagggag cggaaacatc ggccatgcgg tagggaatac
     4441 tgaaccggtg gtatttttct gtcgccgagc cgtggtaggg caacaggttt acttccggtc
     4501 tcgaagggaa acccgacagg aactcacctg tggcctccat gttctcaagg ctgtcattta
     4561 ttcccggaat aactggtatc cgaactctta cattgtgctt ttcgctcata agccaacgaa
     4621 aattctcaat gataacctcg tttggaacac ctgtgtactt cctgtgaagt gaagtgtcca
     4681 tcatcttgag gtcaaagagg aaaagccggg tataaggggc aacctgcttc ataacatcca
     4741 gcgtggcaaa cccgcaggta tccacaacag tgtggatgtc cctcctgcaa cattccctga
     4801 ggacctccac aagaaaaacg ggctgggaca aaggttcccc gcctgaaaag gtgactccac
     4861 caccggactc gtcgaaaaag aggagatcct tttctatctc cttaaggatc tcttcgacgc
     4921 cgacctcccg gccgatcatc tccctcgcct cggcggggca ggcccgtaca cactcgccgc
     4981 agcctgcgca gcggtcggga tcggcaacgt aaccgcgctg cgtcaacgag atggcacccg
     5041 cagggcacgc cgtcacacag gctccacagc ctatgcagcg gtcgccacgg tagctaatct
     5101 cggcaaacgc cgactggctc tctggattat ggcaccacca gcacgacagg ggacatcctt
     5161 tcaggaatat ggtggtcctt attccgggcc cgtcgtgtat tgaatagcgc tttatgtcga
     5221 agatgatgcc cctggtatta ccgttcatct aataacctcc gcacggatcc cctcgaggtt
     5281 cctttctttt tcgagctcgc tgtagtatct gcgaatccag ggtttctgga cctcgaagga
     5341 ccagtgaaca tctcgcatat agtccgaggc agcctttgca tcccccgtct ccagaagttc
     5401 gatgaaacgt tcgtgttcgc cggtggatgt aatctcccac tccttgacaa agcccttgcg
     5461 cctcgggaag tcgtagagcc tctgcttcag ggtcatgact gttcggacaa ggctctggtt
     5521 cctggaaagg tcaaggtaga tgttgtggaa ggagatgttg tgacggtaga aggtgtcaaa
     5581 atcatccctg tccagcgctt ccctcataag cagattcagg cgtagcatct cttctgtctc
     5641 ggtcaaggtg atgcgatccc gctcgttgaa cactaccgag gcctccaggg ccccgatgat
     5701 ctcgtaggca tgacggatat cctcttcaga gagggggttc acgatgatcc ccctgcgggg
     5761 gtatatggtg acaaaaccct cggtttcgag ttgaagaagg gcctctctca agggagttcg
     5821 gcttatgccg aaccgggaac tcatctcacc caggttgatg gcactgcccg gacgcagctc
     5881 gccctggttc atctggctcc gaagatagtc gtaaacctgc tgcctgagag acaccatgtt
     5941 gaaagggcag tcgggggtca tttcgcacct cctctaaatt aagatatatg atatatcaga
     6001 tatcagcaaa gggcaagcaa ggatcccgat cccgcgcctt tggcttatac ttgaaaagta
     6061 cgctctcctt gccgagaaca ctacaatgct caggggggaa tattccatga aaagggtcag
     6121 cattcttaat catgttatag gtccaataat gagaggtcct tccagctccc acaccgccgg
     6181 accatggcgg atcggacgca cggcccgtga ccttcttggg tcgagaccta aagaggccat
     6241 cttcactttc gaccccaaaa gctcgctggc agtctgcttc cacgaccagg gaagcgatgc
     6301 cgcccttgtt gccggtctcc tcggattcac gctgaccgac gacgacgaac ctcgcatcct
     6361 ggagatggct ctcgaagagg ggcttgacgc caccttcagg cttgccccgt ttccagaggc
     6421 ggaccacccc aacagcactc tccttcatct caagggggaa gagggcgaag aactgactct
     6481 tcatgcccgt tcagtgggcg gagggtcgat gcagatcgct tccatcaacg gcttcccggt
     6541 tatgataaag ggggaccgct acgaacttgc tgtcgaatgc gaagcatctt cgacagaaaa
     6601 ggtcatctcc ctgctcaaaa cctgggaccc tgacgccgcg gctcagacag acaaaggcag
     6661 gggctttgcc cacggctcct catttctcaa gcctgaggag acgcttctgt cctctataaa
     6721 atctcttacc ggtgtaggcg ggttctacaa ggccgatccc gtgatgcacc ccctcagagg
     6781 taaagaactt ttccccgata ccgggggact tctggccctt gcgaaggaaa agggatggtc
     6841 ccttggcgag gcagcccttg agtatgaaag cgccctcctt gaacttcccc gtgaacaact
     6901 gatggaagaa atggggatcc gcctcgatat aatgcttcga gccgtggagc tggggctaaa
     6961 gggggaccat cttgagatga agctcctgag gcctttcgct ggcagggtca tgaaagccga
     7021 agaggccggg aagatgttcc tggggggccc tcacgccagg gccgcgatca gagccatggc
     7081 ggcgatgcac attaacggag cggggggggt tgtatgcgcc gctcccacag gcggttcggc
     7141 aggagttatg ccgggtgtcg tttctacatt gcttcaggac atggaggtaa cgcgcgaaaa
     7201 ggtgatcatg gccatgtggg ctgccggagc gataggcctt gccctagacc gacggagcac
     7261 cttcgcagcc gaagtggcgg ggtgccaggt tgagatcggg gccgcaggcg ccatgagcgc
     7321 ggccgccgtt gtcgaaacgg ccggcggatc cgccgaccag gggtgtgccg ccgcggccac
     7381 ggtgttccag aacatgatgg gatctgtatg cgacctgatc caggggatcg tcgagatccc
     7441 ttgccacagc cgcaacgctg ccctggcctc acaggcattc ctgtgcgccg atcttgtgct
     7501 tggcggatac gagaaccctg tacccctgga cgagacggtg gacgccgtag acagcgtcgg
     7561 ccggatgctc ccggaggagc ttcgctgcac ttcaaggggc ggcctggctg tctgcccttc
     7621 ggccctgggg ctttgcagac tggaccgctg cacgaaataa taatcaataa ggaagagagg
     7681 aggatcagca tgcttgtaat atcaggagag gaaatacgca ccatctaccc catgaaggac
     7741 gccatcgagg cgacaaaaca tgcctacagc cttttttcgg aaggcaagtc cgatgtacct
     7801 ctcagaacca gcctcaaggt aaaggaacac aacggcaatg tgctgttcat gcccgcctat
     7861 atcagtggag aaaaccccct ggtggggatc aagatagttt cagtttatgt cgataacccc
     7921 ggcaagggac ttcctgctat tccctcgact gtcctgcttg tcgacgccga aaacggcttc
     7981 gtcaaagcaa tcatgaacgg tacagtcctc acacagatac ggaccggggc cgcaagcggt
     8041 gcggctacag acctccttgc gcgtcccgac tcgaagatcg gagcgctctt cggcgccggc
     8101 ggtcaggcag agtttcagct ggaggccatg atcacagcca ggactcttga agaggtgcgg
     8161 gttttcgatc cacgcatcga aaactgccgc tcattcatcg aacgcatcaa ggacaaagaa
     8221 cccttctccg gggtacgcct cgtcgtggct gagacttccg acgaagcagt gcgggatgcc
     8281 gatatcatta ccgctgctac gacggccaaa agaccggtct tcaacgcggg ttccgtcaaa
     8341 ccgggcgtcc acatcaacgg agtcggctcc tacactcacg agatgcagga actggatccc
     8401 gggatactga acagggcagg caagatctat atggactcca tcgaagccgt cctggcagaa
     8461 tcggccgatt tcatcaaccc ccttaatgag aagaccttct cccgcgattc catcacaggg
     8521 gagctgggag agctcgcgca aggcaaaaag ccgggtcggg catcttctga agagataact
     8581 cttttcaaga ccgtcggcat cgccgtgcag gatgtcgtcg cagccgagga gatcttcaaa
     8641 cgggctgtaa ttcacaaggc gggcaagacg ataagctttt agacacgatc aagatagatc
     8701 gattcgagat attcaggcag acacagaaaa gaagttcaag gttaaatgac atcaagtcca
     8761 ggcagtagac agtcattgat atttgatggc tgtctattgc cgcccctctt cacccttgtt
     8821 tttctgacat ttgttccagg cttctgaact gttgcaggcc gcttcacgag gtggacttat
     8881 gggttgtgac ttctccccaa gattttctgc tattaacgag gccgcgttga ggaccatcgg
     8941 ggcaaaacct tccatgatct ggagggttag ccttaccgtt ggaccgccga ctgttattgc
     9001 agcccggcat tcccccctgc tgtcaaagat cggggctgcg atccccagga taccgggaat
     9061 aacctcctcg gcactgatcg aataaccttt ttcccttatt cgccgatatt cttcgcgaag
     9121 tttcaaagga tcgagtattg tctgttctgt ccacttttcc agcggctcct gtaaaagcgc
     9181 ctcgatcttc tcctcagctt caaaagcggc aatgaccttg ccaagagctc ccctgttaag
     9241 tttgatgacc gtcccaacct ggttgtcaag acgcagtggc tggttgctga ccaccttgtg
     9301 gacaagaatg ggtatcccat cttccttgac gcacagccct accgattcgc cggttttttc
     9361 cagaagatcc ttcataacag ggtcagcgac atcgataatg ctcctgtcac ccctgtaggc
     9421 gatagctatc cttgagagcg ccggcccaag catgtatttt cttgatcttg tgtcctggga
     9481 gacaaacttt tccctgacca gggttgacag gatcttgtgt ataccgcttc tgctgaaacc
     9541 catttcatcg gctatctggg agatacccat ttcatggggg ggttcgccca gccttttcag
     9601 gatccaggtc accttttccg tactgttcat tatggaggtc tccccttttt tatgaaaggt
     9661 cctgatccag gccgatcccg atccctctct gacaggttga cgcggatagt ataacatgct
     9721 aaaaatacca taagttttgc tataggagaa acggttcgct acaggagaaa tggaggtgct
     9781 gtattgcctg aaagagaggc gttcctgaag gctgcagaaa acgcaaggga aaggatcatt
     9841 ggatggagga gggattttca ctcccacccg gaactggcat accaggaagt caggaccgcc
     9901 ggtatcgtcg ccaatgtcct cgattccctg ggttacactg tcgagaccgg cattggcggt
     9961 acaggtgtag tggcaaagat caagggggtt ttgccaggaa cctcaagggg gctcagggcc
    10021 gacatggacg ccctgccaat tgcggaagag acaggtctcc ctttcagatc aggcaacgaa
    10081 ggaataatgc acgcctgctg ccatgatggg cacgtggcaa tgcttcttgg ggcagcagaa
    10141 atgatcgccc aacataagga gcaactcaag ggcgagatag tcctggtatt ccaaccggcg
    10201 gaggaaggtt acgcaggggc gcagaaaatg atagacgacg gcttatttaa aaagtttccc
    10261 gtcgattttc ttttcggtca tcacattatg ccggtcatac tcccccacaa ttcaataacc
    10321 accaggaaag gggcatttac cgcaagcagc gaccgtttcc atttccggat caagggttac
    10381 ggcggccacg catcaatgcc ccatctgacc aaagacccgg ttcctgcgct acagtcactt
    10441 atcgggggga tcaacagtat aaccagtcga aatacagacc cttttgacca ggctgttata
    10501 tcaatcggtc atatcggagc aggaacatgc tacaacgtca tcccggattc cgctttcatg
    10561 agcggttctg taagaacact ctctgaacag agccagcaac ttgtccagaa aaggatgaca
    10621 caactcgcta aaggcataag tgaagcacat gaggtcgaaa tatccatgga atacaaggtc
    10681 aattacccaa ctctttttaa tgatgccacc cttaccggaa gaacccttga acttgccaga
    10741 acattctggg gtgaggagaa agtaattgaa aagcaagatc ctttcatggg atcggaggat
    10801 tttgctttct actcaaaatc tgtaccctgt tgctttctga tgataggagt tgaaggcgaa
    10861 ttcggggttc atcatcccaa aatgaccttc aacgaatcta tcttgcacaa aggggctgca
    10921 tggcaggcct ggcttgccct ggaaagcgga gagatcagtt tgctctaata aaaaggcaaa
    10981 gggggtaaat caaatgcaaa aggtcctgga acagttcatt tttctcagta tttttctact
    11041 tgcgggttat ggagtccgtg tctggttcaa acctgtacag aaacttttcc tcccggcaag
    11101 ccttatagga ggctttatcg gtctgatatt gtgtccaaac ggtttcggga taattccggt
    11161 aacacccgaa atgatgaaag aactcggggc attgcccggt atccttatca cagtagtaat
    11221 tacggcttca gtgctggggg tcaaactgcc aaaaccgggg gaactggcag gcggacttgc
    11281 ccgtaacttt atgcacctga acgtattttt tatgacccag atcggggtcg gtctgcttgt
    11341 tggcgcagtc ttcatgaatt ccacgtacaa aactttcggg atcgaacttg tggctggttt
    11401 cgccgggggg cacggcaccg ctgccatgtt gggcagctcc ctgaaagaca tgggacttga
    11461 ctggtggcta aacagtcagg gggtaggcct cactaccgcc accgtcggaa taatcggcgg
    11521 catattgatc ggcatagggc tggtgacata cgcttccaaa aaaggatttg tatcaggctt
    11581 caagggcatc gaccagttgc cgaaagaaat gatcatcgga tactcaaagc ccgaagacgc
    11641 aaaaccaatg ggaaggcaga tacagtatcc cgctgctatt gagccgtttg ccttcatcct
    11701 gagcctggtg ctcatttcgg tcggtctggc cttcaagttc agggtcatgc taacgggaac
    11761 tgtcatgttc cgggtggcac cctgggcatg gggtatcata atcatggcta ttatctggat
    11821 gctgatctgc cgcctgcgac ttgactggct gatagatcct gtagcaaaag ccaggctgat
    11881 gggcaccttc gtggactacc tggtggtagc ggcaataatc tctcttccag taagagcggt
    11941 ctggcagttc atagttccga taacggtgct atgcgctgtc ggtctcgctg ttacaatttt
    12001 tgtcaccatg ttcctgggaa gaaaaatgct tccggagaaa gactggttcg agcgaagcat
    12061 tattagcttc ggccagtgca caggagtcgg ggctaccggg atccttctgc ttcgccttgt
    12121 tgaccccgat ttccagaccg aggccctcag ccactggtct cttgcatttt ccgtaatatc
    12181 actttatttc tggttccttt tcgcctcgct ccccatcctt gtaatgaacc acggcctgct
    12241 acttacagga ctgttcttta cgggcctttc actggccata gtggtcgcaa gccgcgtgat
    12301 cccgggtttc tggaacacgg acacagccac cggaagttag ataaccccga aaaagaaggg
    12361 ggctggaacg ataattatcc agcccccttg tcattatgat ctccagaccc cttcctgaaa
    12421 ccaaaaccgc tggatatcct gtcagcagtc tcaacaacga aaggtatcaa ttggttcagc
    12481 cggatatttg tcaggcgtac agtaggaccc gctactgcca ggcttgccca gacctcgcca
    12541 tgcctgttgc ggacaggggc tgcaatagct cttatcccct cgaaactctc ctcatcgctg
    12601 gtagcgaaac cctgcttcct gatcatggca tattcctgga gaagaacatc cggatccgtg
    12661 atcgttttcg gtgtcctgga ttcaagggga cccgaaaaca gatcctccct gattctttcc
    12721 tccggctcat aggccgccat caatttaccc tgggctccac gattggggcc gagacgggag
    12781 cctatcttgc cttcatacct cagcggctgg tcgctgacta tctggtgtgc cagaaccgca
    12841 atatcacctt cacggataca aaggccaacc gattcccccg agcgggaggc caggtccctg
    12901 aggaagggtt ctgcaacctc gagaatgctt ttttcctgct tataggtgtt tccaaggcga
    12961 aaaagaactg ggccaagaga gtactttttt gtcagtggat cctgaacaac aaagttctcc
    13021 ctcaaaagga ccatcattat tttaaaaata ccgcttcggc ctgtacccat ctcccccgcc
    13081 atctccgaga gccccatctc gaaagggggt tcgccgagcc ttttaagcaa ccatgccatc
    13141 ttctcaaaac tgttcatcaa acaaccctcc cgatcacaag atagcattga caattatcaa
    13201 gacccgtggt aggaataaac agtcatgttt cccatatgat aacctgtttc ccataagagt
    13261 acaacccatt ggggggatcg atttgtccga ttgcagaata agaagtatat ctacgacggt
    13321 acttgaaatg cctttcagga agacatggca gatctcgcta tattccgcag caacccgccc
    13381 tcaccttttt gtcagagtgg aaacagaaga tggcatttac ggctacgggg aggcatcccc
    13441 ttcccccgcg ttcatgggcg aaaccgcata tacagcgagg gaggtcattg atagacatct
    13501 taaagtggcc cttatcgggg tctcgatcca tgaccgctcc actattcata ccagaatgga
    13561 ccggatcctc cacggcaaca gcggagcaaa gacggccatc gatctcgctg tccacgattc
    13621 agcagggaaa gcactgggcg ttcccgtttg tcgccttatt ggcgggaaac ttagggaaaa
    13681 cgtcccccag gcgtgggtac tgggtattca ggaagtaaag gacggcctgg aggaagcgcg
    13741 gtttatgctg gaaaaaggat acggaacgat caaggtaaaa gtcggaacca accctcccag
    13801 ggagatcgaa cttctcaaag cccttagatc cgagcacgga aatgacttca agctcagggt
    13861 agatgcaaac cagggttttt ctgtccgcga aaccatggca ttcctgcaaa aaacaagcca
    13921 cctggatctc gaatgcatag agcaacctgt tccaagatgg gacataaaag ggctgcgtga
    13981 aataaggtcg cgcggaggag ttccagttat ggccgatgag agctgttcgt cgccgcagga
    14041 cgcgatcagg gttattgagg caggggccgc ggatattctt aatatcaagg tcgccaaggt
    14101 cggcggactt tacagggcag tccagacggc atccctggcg gaggctgcag gactttccgc
    14161 ggtcgcgggg agcaatatcg aagctgggct gggttcctgg gccaacatcc acttcgcggc
    14221 cagccaggca tgcatgtgcc atgacaacga ccagatagct gttccgcttt atgaaaatga
    14281 cattattgaa gaagagttcg atctggcctc gggtgggatc ccggtaacgg acaaacccgg
    14341 attgggggtc agcctgtcaa aacagatcat caatgaactc cgttgacggc aaaaagtctt
    14401 gaacgaaaaa atacaggaaa gagagggata cgagatgctc aacaacaaaa acgcccgcag
    14461 aatgacagtc ttggtaattg cttttctgat ttccgttttt ccagtgttca gtggcatgac
    14521 tttggcggaa gctaaaacca tcaccctgag ttatgcggaa tcgggtcccc cctcgggact
    14581 tgggggcgct ttcgtcaata ttatgaaaga ggagatcgaa aaggcgaccg acaaccaggt
    14641 aacaatagag gttttctggc agggttcgct tctcaagggc aaagagatcc tcgaaggagt
    14701 aaagaacggc atggtggata tgggttacgt tcttccagca tattttccga aacaactctt
    14761 tgtccacaac gcttttactc tttttccaca ggggccaacg ggcttcaaaa acgtagtatc
    14821 ggcaattaca aggagtttcg aaacccttcc ggaatttggc gaagagttat caaaatggga
    14881 tcagagggta ctttatatgc gctttatgct ccctatggcg ttgacctcca ccaggccggt
    14941 tgaaaagctg gaagacttca agggcatgaa gatccgcgcc tccagctcgg cttacctgaa
    15001 ggcccttcag ggagcggggg ccaaccccgt ctccgttccc tggggagatt gctatatggc
    15061 tctccagaca gggactgtgg acggcgtttt cactaatttc gacgggatcc atgcgaccaa
    15121 actccacgag ccgggcagac atatcttcac aacaaggcag ttatgggtgc cgatgcctat
    15181 ctttgtgaca ataaacaaca aaacctggca gaaactgcct gaagatatcc gcgataaaat
    15241 gcttaaggca caggagccga tccttgcccg ttttgctgaa cttttcgaaa aagagtggga
    15301 tcggatcgta gaggatcaga aaaaagaggg cgcaacgata gtcagggctt ccgataaaga
    15361 tattgaagac ttcgtgtccc tccaggcatg gaatgaccta agggccgaat ggtcagcaga
    15421 ggcacccgcg atgggagtga aagaccccca acatttcctt gatactcttg gaaagatcat
    15481 aggtgaagag atcgacaagg agaaaaaaca gtagccaggg gatgatcaag cctgtttttc
    15541 gaggaggggc caggtagatg ttcaagcaaa taattcacca gatcaacaac gctcttgcgg
    15601 agcttagcgg ctggcttgtc acggcgataa tggctctgct tatcctggac attatctcaa
    15661 ggttgtcagg aagggcggtc cagggtgtca gcgagttcgc cgtctttgcc cttgtagcca
    15721 cagtttacct tggcctctcc cattgtgaag agaaggaaag tcatctcagg gtagagttct
    15781 tttctgaccg attcaaggga cgttcgaaaa gaatatcgga caccttcgat tacgcagtgg
    15841 cagtcatttt cgtctctgtt gctctctacg catcatggcg cagcgcattg ttctctttcc
    15901 agacaggtga atccgtcccg gggacaatac ctttgcccat ttaccccgcc aagttcgtcg
    15961 tttttacagg tctgcttttc taccttgctc aattgatcat aaatcttgga tcaaaatgga
    16021 ccggcggtga taactgatgg gaaacgtaat gatcggactt cttgggatac taatcctcct
    16081 tgcccttctg ggtaaaggtg tccacatagc gatcgacttt ctcgtagtcg gcttcgtcgg
    16141 catatctgca cttgtcggga tagacacggc aataagcttc ctggggcaga cgctatatca
    16201 cacaattgcc acaccctctt attgtgccct gcctcttttc accctgatgg gcgctttcgc
    16261 ttcaatgggg gcttttgcgg agaaggcctt cgagggcatt catgatctta cccgcaaact
    16321 ccctggatca ctcgccatag caagctgttt cggctgtgca gcatttggag cggtgtcggg
    16381 ctcatccctt gcaacggcag cgcttttcgg caagatcgtc cttcctgaaa tgatgcgcta
    16441 tagatacaac aaaccattcg ccatggggtg cattgcagct gcgggcggct ttgcggctat
    16501 gatccccccg agtgcaggtt ttatcgtgta tgccatattc acagaccagt ctatagggaa
    16561 gcttttcatg gccgggatac tgccgggaat acttaccgca gtggcttttt cagttatgat
    16621 cgtaataaag gtcatacgaa atcccgaact cgccccacga gagatcgaaa gatcacacga
    16681 taaaaaaacg ctgttatcaa ctttcggttc catcaaaaaa atgtggtcca ttgcccttat
    16741 agccgctctc gtacttggcg gtatatacac cgggatcttt acccccaccg aggctggagc
    16801 ggtcggagcc ctggcagcac tgcttatagg gtttcagcag gggaagatcc gatcaatatc
    16861 tgtcatacga aaggcgatgc gcgagtcggc ctacacgaca tcgatgatat tcataataat
    16921 ggtaggtgcc ctgttctttg ggcgctttct tgcgcttacc cgggtcccca cgtgggcttc
    16981 catgcttatc acaggatggg acgtcccgag gatagttgta atggggggca ttctcctggt
    17041 ctggttcctc ctgggaatga tcattattcc gacggggatc atggccctga ccctgccaat
    17101 atttttccca ataatcgtat cccttggata cgacccgatc tggttcgggg taataataat
    17161 gaagcttggc gagatcgcag cgatcactcc ccctgtcggg ctaaacgttt atgcgataaa
    17221 aggagtggcc ggaaaaggga ccacgctgga agacgttttc aggggtgtat ggccattcgt
    17281 tatctgcgaa ctcgttgtcc tggccattct tatcgctttt ccgcagatct ctctctggct
    17341 tcccggtatg atgtagctgt tactgcgctc ttcggctgcc gctgctacaa ttgccggggg
    17401 ggatgaagat gaaaaaagtt atcgcgctca aggtagatgt ggataccctt aaaggatacc
    17461 tggaaggggt cccggccatg ctttctctgt tcgagaggca cggcgtcagg gcctccttct
    17521 ttttttcctt tggccccgac aactcgggca aggcaatacg acgggtcttc aggaaggggt
    17581 tcatccagaa aatgttgcgc accaacgcgc cgggcacata cggtctaaag acaatgatgt
    17641 acgggactat cctcccggca cctctcatcg tgccgcgcaa accggatatt ttccgcaggg
    17701 cctttgacca gggacacgag tgcggtatcc acgcctggga tcatgttgcc tggcaggaca
    17761 cccttgatcg catgccggag aaaaaaatgc gtgatcactt ctcacgggct tttgaaatgt
    17821 tctcctccat cgcagggcaa agaccccgct gctgtgccgc ccccgcctgg aaagtcaccc
    17881 ccgcgagcct caccatgcag gacgagtacc gtttcgacta ctgcagcgac acccggggcg
    17941 tattcccctt tatgccgatc atggagggca cagtcttcaa aacacagcag gtgccatcaa
    18001 ccttgccgac catggacgaa attctcggct ctgaagggac cgacgtgaaa aacattaacg
    18061 atcattacat ggaagccatc aaacctggac tgaacgtcca cacgatccat gccgagatgg
    18121 agggcggtgt aatgatccat cttcttgaag aactggttgc caggtgcctc aatgaaggca
    18181 tctcctttat gacgattggc gaagcgatca acgggaacga tcccgcggat ctgcccttgt
    18241 caccgataac caaccatgaa ataccgggga gagcaggagc tgtggcggta caatccctgc
    18301 agaccaaggc ctgagcacag caaaatctgg acaaacatag ccccttgggg tatattatca
    18361 tcaaggatac taattttcgg tttcaaaggg agggacaaac catggaaaag ggttcctttg
    18421 atatgaaaaa agccctcgtc tacgccatcc acgcagagat cgaagccagt gaattctact
    18481 cggattgggc aaaacacgcc gaaggacatc ttaaaaagga acttgaggaa ctttcaggct
    18541 gggaggggga acatcgcgat tctctggtga aatacctgga gaattccttt ggcgagaagt
    18601 tctcccgtga tcctgagatg gtcgtggacc ccgcgctcaa ggttcaggcc gatgagttca
    18661 ggaacaacta cgctctatta agaatagcct caacggtctt cctttcagag atgagggcca
    18721 tggaacttta cgaaaagatg gaaagcgaaa gtaccggaga ggcaaagaaa atgttccgtg
    18781 aaatcaagga gatggaaaaa gggcacatgg attcggcaaa aaaacgctac atggaactga
    18841 gggagcatat tgtcggcttc cacgctttct agatcacggc ggtaaagatt tttccggata
    18901 aaaaaatgcc gggagccaat cggcccccgg tatcttttta tctttggatt agatgcaggc
    18961 cggctcgcgg aagaggcagc cttcctcgaa tcggctgaaa cggaaatctt tgacgtctat
    19021 gaaaggttct tcgcccaaga tcatctctga cattatgcga ccggcagaag gggcgaactg
    19081 aagcccatga ccgctccagc ccccgtcaaa gtagaaattc tccaccgggg ttttgcccat
    19141 tattgcctgt tcgtcaggag tgatcgcata agggcccgtc cactggcgga ccaccctgac
    19201 tcctgccagg cagggcattt tttctattac ccttctggaa acctcctcga ggaagggcca
    19261 ggaagattcg taactggtat ccttcttctc ctccgggttg ccccacccaa gcatgaaggt
    19321 cccgttgggg cactgcttcc agtaacttcc gtcatcaaga caaagggtca tggggtgctt
    19381 cattctctcg acaggctccg tgatcaggat ctggtgccgc tccgcgatga tcgggacctt
    19441 gagtccgcac catgaacaga cctcgggcgc ccaggcgttt acggcacaca ccactgcccc
    19501 tgcctcccag tctcccttgt cggtctttac accccagact ttctcattat cggtgagaag
    19561 accagtaacc gctgttttgg tatggatctc cgcaccaagg cgccttgccg catcgccgta
    19621 ggcaaagttc atagtgtggg ggttcacatg accgtctttc tggttgaaac tggcacctag
    19681 catcccttca acgttaagtc ctggtgcaac ttcctttata cccgccggat ccaggatcac
    19741 ggaatcaatt cccagagaat tctggagagc cacgttcttc ttcaattgct ccaactggga
    19801 ctcgctgtag gctatccaga gatagcccgc ggggtcatac tccagatctg ccttgtactc
    19861 cagttcctcc tgcaaatggg gtatcttctc catgttgtac atcgaaagac gacagttgac
    19921 ctctgttcca aactgatacc tgaggccggc tgcgcttctg ccgctggaac cagaggacag
    19981 gtaatccctc tcgaaaagga ccgcctttac ccccttttta gcaaggtgat atgcgataga
    20041 acatccgtga acaccgccgc caattattgc cacatccgca gtatttcgcc ccattgatcc
    20101 cttccccttc cacggaaaaa ataaagaccg gataattgcc acaatcgtcg ctcgtgcata
    20161 taatcttaat gacctggtat ataattttat ttacttgtga cccaagagtc aaaccaggtt
    20221 tcactctttt ggtacgacct tcacatacga aaaacccttt atcctgagcc tcgcaacaga
    20281 gaaaggagaa atatcagtca tggagataac aagagccaca gcggtagtcg attctcacac
    20341 cggaggggag cccacaagga taattatcgg cggcgggcca tacctccccg gcaagaccat
    20401 gagcaaaaga tgggcatacc tgagggagaa tctgtcggaa ttccgaaatt tcgtgatgca
    20461 tgagcccagg gggcacagcg acatgttcgg agcattcctc acacctcccg agagagaaga
    20521 ttcccactat ggagtcattt tcatggattc cggtgacggc gtctcgatgt gcggtcacgg
    20581 atccataggc accgccaacg ccctggttga gcttggaatg gtcccaagga ccgaaccttt
    20641 caccgaggtc gtcctcgaca cacccgccgg acagatccgg cttaaagtgg aggtaaagga
    20701 cggcaaggtg ggcaacgtaa ccctggcaaa cgtaccttca ttcgtctata aaagtgacgt
    20761 ggaacttgca ataccctccc ggaacaaaaa gatccacatg gacatagcct tcggcggcaa
    20821 cttcttcgcg attgttcacg cggaggaact gggccttacc cttgagcgtg aggaggtccc
    20881 ggaactgctg gcgttaggcc tggagatact cagggaatcg aacaagcaaa taaaggtcca
    20941 gcaccctacg gaaaaacatc tcaactacat aggcctcacg gaattcgcgc tggaacgccc
    21001 cgggaaaccg accaaaaact gcgtcatctt cggagagagc aacgtcgacc gttccccttg
    21061 cgggactggc acctgcgcaa agatggccct tcttgcagcg aaaggcacac tcaaaccggg
    21121 cgaggtattt gatcacgaaa gcgtagtggg gagcgtcttc cacgggcact accagccggg
    21181 gccgaaagtg ggggattttg aaacgatact acccttcata aggggtaaag ccagcgtgac
    21241 gggtttcaac tttcttctcc agcagaataa cgacgaaatg ggttcagggt tccttctgcc
    21301 gaaataaagc gattctggcc ataggggtca aacctgagcc ttgaacccct atggccgcct
    21361 atctttcgat gcggttcttg ccgccccgtt tggccttgta caacaacttg tcggccctct
    21421 ctaccaggct ggtcggggaa tcaccgtcaa actccgcgac tcctccgctt atggtaacct
    21481 tcgatccggg aatgtccaac ttcagttcgc tgacgccctg acggatccga tcggcgacct
    21541 ttgcggcagc ttcgagacca gtctcgggca ggatcaccaa aaactcttct ccaccgtaac
    21601 gccccgcaat atcgctgctg cgggcacaac cctgtattgt tctcgcgacc gcctcaagca
    21661 ccctgtctcc gaacacatgg ccgtaggtgt cattgagttt cttgaaatca tcaagatcca
    21721 gcataataac cgagagattt ttccctgcga cagatgtgcc ctccacaagg agttccagca
    21781 tttcaaaaac ataccgcctg ttgaaaagtc cagtcagacc atccgtctcg gtaagttttt
    21841 tcagttcttt tgtcctctgt tctatccgag actcgatatt ctggttaaga tcctctatct
    21901 tccgtgactg gtcgctgacc ctgccctgca ggaggtcgtt catttcgcga ataccatttt
    21961 ccatccttgt tcgctcctgg atcattttat tggccgcggc cgcaagttcg gcaaattccc
    22021 tgtatctaaa gctttccggg ttgatctccg aataatcgac agaggctttc tcgaagaaag
    22081 agaagaagga ggaatactgt ctccttactc tcatggccac gaactgtagc atcacaaggg
    22141 ctatgagtat caggaaaagt accgtcaccg acatctggag gaatcttctg agaacctctt
    22201 cctttaatct gtcaccccct gcagcaatca gtccctctat ctcatctgtc ataacaccgg
    22261 ctccgactac ccagccccac tcgggaacag cgataacgaa agccagcctg tccgatgatc
    22321 cacatcctcc actgggggga gcatggtgtt caagaaagat acccccgggg ttttgccttg
    22381 ccgcatctat ctgttccctg atgaccttct ctctccaggc agggtcgtcc ccccaaagac
    22441 agacatcttc tcctcctgcc tgtatgtcat tcaaaagatc ttctccttca aaagaccgaa
    22501 cgaaaagacg cttttctcct ttcggtccga atctcaacga agatacaaag tcaagggctt
    22561 ccctctggat agccatctgc acatcatcga ggtactctcc cgtacctata taccagccaa
    22621 agggctcgaa aagcttgaca aaggagatct tggggctagc ttcttcgttt tcatgtcccg
    22681 gcttcttcca gtagccggtg acaaaaccct caccgtgctc tttgacaacc tggatctcct
    22741 ccctgatgac ataattgccc ctctcgtccc gaaggtctgt cacgtccttt ccctcaaaat
    22801 cggggaaaac agggtaaagt tcctccactc catcgaggct cacaataaaa aagtagccct
    22861 tgccatctct gtaacggata ggcctgaggg cttcctttat cattcttttt gtctccgcta
    22921 ccgacaggcg gtgtccaaac ttctcgtaaa tattccaggc aatagccagt gcctcttcgg
    22981 tccggttgcg gatattttcc tccaggacac tctccgtgcg ggtggtcatg aacctgatat
    23041 ggtctacagg tctttgaacc tccgaactga gaagaacctt ctggctttgc aaataatccc
    23101 tgcggatctc atcggattgc ctgctgaact gctcgtactc cgaccggatc cacataaacc
    23161 ccaggacaaa ggacgtgaag aggatcaggg ctaccatgcc ggcaaaaaca acattgacaa
    23221 ggctgtaatg ttttttcagg accgtaaccc cccatttatc gtttctctta tgtcgcgtat
    23281 atttaaaggc caggagccgg ataaaacaag accatcgagg ttaaaccatc tcgcagggtg
    23341 taaatatttt agcatggtgc ccagaaacag gatcgcaata tttaaggatt acaataagaa
    23401 caaagggtgc taatgaaagc aacaccttcg aagtgttcac tttttttgaa aacaaatatt
    23461 ctttaaaacc gggccatttt tttagaacaa cgaattattc tctaataaat taaatttttt
    23521 acaaagaata ttttccatag ggtaagaaaa ctcaatgtca aacaaggtgt tcgtattcct
    23581 ggaggaaaaa tgaagagaag aaaattgtcc gtcgcatccg taattgcggt cgctgtactc
    23641 cttgctattg ccgggggaag gatctttata catacggccg acgttgtcat ctatagcgaa
    23701 actgaaagcg acaggttgct gggaagactg caccagaaac aaaggggcgg ggttcaggtt
    23761 cagggctccg gagaggttgt gaggatcctt cctgatgaca acaaggggag caggcaccag
    23821 cgtttcatcc tggcattatc cacaggtcag acacttcttg ttgcccacaa tattgacctc
    23881 gctccccgga tcgaggacct tagaacagga gacaaggttg agtttatggg tgaatacgag
    23941 tggaatcctg cgggaggggt catacactgg acccaccgag acccatcggg gaaaca
//
LOCUS       DAOTIQ010000023        23971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2474_length_23971_cov_6.652617, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000023 DAOTIQ010000000
VERSION     DAOTIQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 23971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23971
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_23971_cov_6.652617"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1394)
                     /gene="recA"
                     /locus_tag="P5541_04560"
     CDS             complement(267..1394)
                     /gene="recA"
                     /locus_tag="P5541_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRV71284.1"
                     /translation="MAKRKGSMTREDVLGQALSDIRSKFGEGAIMCLGEGAGPVAEVI
                     PSGVLPLDIALGIGGYPRGRIVEIFGPEGGGKTTLALHALAEAQKQGGIAAFIDAEHA
                     LDPRLAYSLGVKIEDLYVAQPDSGEQALYILETLVRSGAVDVIVVDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISKSNCVVLFINQLRAKISTTGYGGPQETTTGG
                     RALKFYSSLRIEVKRGKSISKGDDAIGHELWIKVVKNKQAPPFRTAHASLIYGKGIPM
                     EVSLIDIALDHDVLKRKGSWIAYKGETLGQGKESVGTYLVEHPELLNEVVSEVKAKAS
                     EGLGFSVPQDDISDDDGDDLLQKAELEIEEGILDIKVDDDQ"
     gene            complement(1398..1982)
                     /gene="thpR"
                     /locus_tag="P5541_04565"
     CDS             complement(1398..1982)
                     /gene="thpR"
                     /locus_tag="P5541_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRV71285.1"
                     /translation="MIRCFVCIEPGDQIIKNLGNWVAEMETVAPSLKWIRNGAFHLTL
                     KFCGEIPFKTVCKIQNALEDGFAFKRLRPFRLELSGIGAFPGIRQPKVLWAGIEGEED
                     QVQRVASVVERAATAAGLDPERRPFHPHVTLARVPPTASLPIGVFSELNSRSDTWGEW
                     TVRSVTLMRSELFPQGAKYTPISVFKFTNDQEVR"
     gene            complement(1984..2502)
                     /locus_tag="P5541_04570"
     CDS             complement(1984..2502)
                     /locus_tag="P5541_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRV71286.1"
                     /translation="MDKITRNQGMEITSGAALVSEACIRGWKICVAESCTGGLAGALL
                     TESEGSSRAFVGGAVCYSNEAKIRVLGVDPGIIERFGAVSGECALAMARGAIKLFGAD
                     VAGAITGVAGPGGGSKIKPVGLVWFCVTFPGGEKVFHRILGGQRADVRRCAADLLVET
                     MLDMVGGDPSEG"
     gene            complement(2519..3010)
                     /locus_tag="P5541_04575"
     CDS             complement(2519..3010)
                     /locus_tag="P5541_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRV71287.1"
                     /translation="MTLSEKWRHRLNPGKWYIPVSTCFGMGYISPMPGTVASIAAVVA
                     AFFIPVSPLLILSVIVVGTIASGKYAAFSGKDDPGEIVIDEVAGMWVALIGLAPGYYV
                     PAFGLFRILDIIKPFPVRNAEKLPGGIGIIADDIVAGIMANLILRGTSWLLFSGGLSR
                     ILA"
     gene            complement(2967..4274)
                     /gene="rimO"
                     /locus_tag="P5541_04580"
     CDS             complement(2967..4274)
                     /gene="rimO"
                     /locus_tag="P5541_04580"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRV71288.1"
                     /translation="MNIFLLSLGCAKNQVDSEILAGILESGGHVLVTDSSCADGAIVN
                     TCGFIAPAIEESVDAILELEILKKSGKIRFLAVVGCLVNRYEKELGAELESVDFWARA
                     GEWESLARFLGGHTSTESQLVLPGYNRFSRYLKISEGCNNRCSYCTIPMIRGPLASRP
                     LSVIIENAERLVLAGAREICLVGQDLTSYGNDISPSFSLCGLIESLEEHFRGEDLWFR
                     LLYLHPARVDEKLVEKVASSDLFLNYLDVPVQHIEERILNSMNRGGLDRKKINSIFSL
                     ARSADPDFALRTTFMVGYPGETEKEFNSLLDFISEAEIDRVGVFPYYPEEGTPAALMD
                     GQVPEDIKQERVDRITLLQEDVSLERQRRFEGRTLKVLVETGDSSEGFAEGRSYREAP
                     EVDGVVEIRSGPLPEPGDFVHVRITEAMEHDLIGEVASSFEPR"
     gene            complement(4285..5160)
                     /locus_tag="P5541_04585"
     CDS             complement(4285..5160)
                     /locus_tag="P5541_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRV71289.1"
                     /translation="MTDIRRTPEELGIEVSERRKKKGLSLSDVSIKTKIRRQFLEAIE
                     NGSLGNLPGDVYSRGFIRTYLEVTDSLDLWPEYELAISGQVPAKGKKAAVQYMPVQKG
                     FQKSSRIWIFGFLLFAAGISLYMIWQQRDAMKAQMASVPDISKSQEVLSPEEIPVSAD
                     VSQETGLAEAIAGETDGVSVTEEEPVLDTSWIPGQEIVETVEESPVVKGSLVIKTNGN
                     CWIRITRADGKTTQMTLNRGGITETVVDVKTSVRFGNAGAVTLVWGGKDIRDIGRSGE
                     VVTIDFLPDGTMKRQ"
     gene            complement(5135..5857)
                     /gene="rpe"
                     /locus_tag="P5541_04590"
     CDS             complement(5135..5857)
                     /gene="rpe"
                     /locus_tag="P5541_04590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRV71290.1"
                     /translation="MAGSLPVKGCPGRKIAPSILSADPLDIAGSIKRLEGEHDWIHLD
                     IMDGHFVPNLSYGPSLVEACRKKWPEAVLDVHLMVEKPENFIEPFAAAGSTIITVHQE
                     ATVHLHRTLQRIVDLGCTPGVTLNPGTPVELLKPVLPLVGLVLVMSVNPGFGGQKFVS
                     AVLEKVRLLCCWREAYGYEYLVEVDGGLGPENSALVSSAGCDVLVAGSAIFNSPDPAG
                     VIREMRAKALEGTGCDGYSKNS"
     gene            complement(5836..6930)
                     /locus_tag="P5541_04595"
     CDS             complement(5836..6930)
                     /locus_tag="P5541_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRV71291.1"
                     /translation="MQRLFKVTLLVLVLVIAGSGIVAFYTIFFGGRDVAVPGLSGMPV
                     IDAVSMTEKMGLLVRIDKVESLQPSGEVVSQWPEPGTRVKKGKIVILKVSRGGDRQPL
                     PDVRGMEYGQAVTRLQESGFDVGDIVRIHSDNRPAGIIVAQSPASPAMVPPGRRIDLL
                     VSLGPPRVGGKVTVPDVLERSEKIARQIVVESGLKVGSVRYEYTYMTPAGMVMNMEPK
                     PGKTLLPGGVVNLVVATGKKPEEPEAPTTEETPAAGDTSPATPGGVPAAPAADSPPTQ
                     PQAPSVGQEPPAQSPTPGDKIARIRYQVPPLSEPMALRIEIVDKNGSRVLMDRQVRTG
                     EYVSLDAPYQGQSSVTIYLGGEFVWQDRYQ"
     gene            complement(6942..8264)
                     /locus_tag="P5541_04600"
     CDS             complement(6942..8264)
                     /locus_tag="P5541_04600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRV71292.1"
                     /translation="MRGLEAALLVWRDVQGGSFASEAIRKRSSSLPESERILATTLVF
                     VALRRALLWKHIARQMTGRPLGTMSQAAGDALVLGIAGVSELRHFLPKVLVNAMVEWV
                     KKSGNQRESGMVNAVLRRAVEEGPAILKKFSTSRSLKDNALAGGVPSWAAAMWSESWG
                     KDRARDLVRFSSMKSMMSLRLSPRADIENTISRLAEGGYRSWRSPLLEESLRLSSSAH
                     PPSLPGYAEGLFSPQSESSMIIGRVVSSIHSGGALLDMCSGRGVKALQILDALPGSVL
                     ECWDVSPAKTRAALSEARRLGISSDRMIAKSGDALTLFPSGETKTVFLDAPCSGSGTW
                     SRHPEGKMRLIPDKIGDMHTLQCALLSKALDIIPPGGSVIYSTCSLFRQENEQVVAKV
                     LESRGDVVEMPPDNTYHSFSRGRPWGNYIFPTLPWLDGFYLAVLNKRV"
     gene            complement(8261..8938)
                     /locus_tag="P5541_04605"
     CDS             complement(8261..8938)
                     /locus_tag="P5541_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRV71293.1"
                     /translation="MPLIFLALIMIFIVPWLGLILLAFFLFLFLLVPLGFAARSLAWL
                     VVGPREFVRVISDPLTRKNHALEHGTINVIEERGAGRLLTGESFKDGFAIRGIADPAY
                     VLDAAREARQRISAGDRALTVNKRCGTTLVVINTISAVIFLLLLLIAGRISILAVILS
                     VVAAYAIGPVASPWVQRHITTDPDFSSLEISGVYLRPVRSVVPVMEVYVHTRVKGRAP
                     DTEVVYQ"
     gene            complement(9069..9758)
                     /locus_tag="P5541_04610"
     CDS             complement(9069..9758)
                     /locus_tag="P5541_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRV71294.1"
                     /translation="MFPFIFDPTFLLLVPALILAVWAQSKVKSAFSRYSSVNARRGVT
                     ASGAVRTILDSNGLQNVSVERIRGSLTDHYDPRSKVLRLSDSVIDSRSIAAIGVAAHE
                     AGHAIQDARGYVPLRLRNAFVPVAGIGSSMALPLFFIGFLFGTPLLMDLGIIFFSAVL
                     VFHLVTLPVELDASKRALSILSGSGLLADDEIGGARAVLNAAALTYVAATLMSALQIV
                     RLFLLRGMRNR"
     gene            complement(9871..11007)
                     /locus_tag="P5541_04615"
     CDS             complement(9871..11007)
                     /locus_tag="P5541_04615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV71295.1"
                     /translation="MDSPVMPSFDLSRNWERVREETLEAIKEVLESQHFILGPQVDAF
                     EREVESYLGVPLAVGCASGTDSLLLALMALGIGPGDEVITTPYSFFATVSCITRVGAT
                     PVFADVEPDTYNISPEGVLEKITERTRAVIPVHLFGQMSRMEVFEAGLKERGIFLVED
                     CAQAFGSTRMIDGKILRAGTIGDAGCFSFFPTKNLGAYGDAGMVTAGSEEVGGKIKSL
                     RVHGSGKQYFHDEVGLNSRLDELQAAILRVRFRHLETWNEERRLVAEIYRTLFAEHNL
                     GELVTPPVEAPGNRHIYHQYVIRCKDRDNLKEELQRSGITSRVYYPLSLHLQPCFSFL
                     GYRKGDFPVSEALSEETLALPIFPELLPEEQERVAEGIAGFYSK"
     gene            complement(11118..11771)
                     /locus_tag="P5541_04620"
     CDS             complement(11118..11771)
                     /locus_tag="P5541_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71296.1"
                     /translation="MIMEPRLYTTSADYVYQELRHRIITKQLKPGQRLPEVNIAVQMG
                     VSRTPVREALRRLSSEGLVLIIPNSGARLASPTKREMEDTYAVREQLECISVATAATR
                     ISERNLRRLEDAILEEEKAFEEKNLELYLEVNENFHKIIADASGNRVLAEFVENILAR
                     TNAYVVFYDPFYDIVDNPSIDQHKAIAQALKDRDREKAVELMKEHLKLSLVGLNKPS"
     gene            11915..12151
                     /locus_tag="P5541_04625"
     CDS             11915..12151
                     /locus_tag="P5541_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71297.1"
                     /translation="MRFFVLVLCLGTLVMYGRAIAGLWSFIIGDPRPWMIVSGLIGGT
                     LSGGIAILLWKKRLRELEWIREPEETTEDGEPLH"
     gene            complement(12143..12661)
                     /locus_tag="P5541_04630"
     CDS             complement(12143..12661)
                     /locus_tag="P5541_04630"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HRV71298.1"
                     /translation="MSLLDFLLAGREERAWLQRRVVSRFGLVLQVSLNIPGIPKRLDG
                     DRDLVEMTGRLIRGNLESAGAVVPCVVTLDNGAGCALLMGLVGADGREVKMIAMKVEA
                     RSWGSVIDIDVIGPEGTLHGESLGRDPRRCLLCESAAKECAREKRHDINTLREAASRL
                     VNEGIRDLSGSV"
     gene            complement(12666..14015)
                     /locus_tag="P5541_04635"
     CDS             complement(12666..14015)
                     /locus_tag="P5541_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV71299.1"
                     /translation="MIEIPLEPGGMTPLYRQIAGHLRRMVQNGTLSCGDRLPGSRELA
                     QSLGISRTTAVMAYDLLEDDGFIERKGRRGSFVSLKRAQSLIAPSDTGPIWDLASGLP
                     SGDLVPWERVASITRENISFIGPGFMFDTPLEGSAGLRKALVRHAARRGIPAKRDEAI
                     VTSGARHALSLTIDAMALKGVRRLWIEELTYPEAVRMAAGSGIDVTSVPMDQDAFVES
                     LSRIERGEVLYLVPSFHNPTGRTLPIDIRKEVLSSASKRGFLVVEDDAYGELRFCDSS
                     VPALKSIDTENRVIYIGSFSQLLFPGMRIGYVLLPDDIRDEFLKIMERTSGSTSSLVQ
                     NVVHAFIEDGSLESSLENARRSMSERMATLCREIGEKLPCFTFEQPQGGIYLWLGTHG
                     LGGEEAARLASEADVAVVPGRHFVNPNRDIEAVRISVSRVSPAMIIEAVQRLSRVWG"
     gene            complement(14065..15132)
                     /locus_tag="P5541_04640"
     CDS             complement(14065..15132)
                     /locus_tag="P5541_04640"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV71300.1"
                     /translation="MRVAACSPFSPQATFSEVRLDKNENPFDPPPGFIEELRHSLSSL
                     EFNRYPDQEAEALRSALSVHCGFPADWISIGNGGDEILLYLIMAFVPDNGCLMTLSPS
                     FSGYEHISRALGTRTRSVPLCFDGSRVFLDEEKLADAISAASPDLILIDRPNNPTGLS
                     VSTEMLSRVAGLSKGVLAVDEAYVDFCGGSFLSEPEKLPPNVFVLRTFSKAWGLAGLR
                     VGWGVSRPCLRERIEKIRPPYNVSVFSQEAARVALGYSEWMQARVKGIAFTRDRFIEE
                     TNRIPGWTALPANGNFVTLYSKGPVDPARAAFEKRGIKVRFPEMGSLSEYSGTWVRVA
                     VGLEEEMNAVLETLKDLSGRL"
     gene            complement(15585..16193)
                     /locus_tag="P5541_04645"
     CDS             complement(15585..16193)
                     /locus_tag="P5541_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV71301.1"
                     /translation="MIDKCSDGKAVFSRDNSLFPVITTKRLVLRQLSRDDAPMLHNIW
                     SDPVVLEFMVLDPFETLEEAIAMIELLDGLYETGTGVRWTVTLASDGTVMGTCGYHNW
                     AKEHFRAETGYELGKDFWGKGYMKEAMSAILEFGFKEMDLNRVEALVTDGNERSLSLL
                     KSLGFKVEGLLEEYEWARGKFQDQWICSLLRSRESEKPNSSA"
     gene            complement(16202..16921)
                     /locus_tag="P5541_04650"
     CDS             complement(16202..16921)
                     /locus_tag="P5541_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV71302.1"
                     /translation="MRKFVVFDKVFELFPQARVCGLVIRNMDNGIPWDGTGMLEQACR
                     EAATRLGDAPFAESSFFAPWREAFRAFGAPKGYRSSVEALAKRAVKGSCPPSINPLVD
                     LYNATSLKFLFPCGAEDLDALIGDVRLIQARGGEPFLPLGSEENDPPREGEIVYLDDA
                     GAICRCWNWREADRTKLTPETKRALLFMESLAPERDEDLRKALVFIDEAIRNNLGAAT
                     TKFWLDSKCGQVDIPEGSWKE"
     gene            18040..18417
                     /locus_tag="P5541_04655"
     CDS             18040..18417
                     /locus_tag="P5541_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71303.1"
                     /translation="MPGKPSLSLSPSPSPCLFRSPFTVHALDPSVILSEAASPSRRLS
                     APYAHRRGSGSDPDKTSNPGLNIHSFGPPWFLALLGNLGVAYREAWSLSVTGESFDFG
                     DELTQACLGSCKKAQAAFREKYR"
     gene            18638..20227
                     /gene="otsB"
                     /locus_tag="P5541_04660"
     CDS             18638..20227
                     /gene="otsB"
                     /locus_tag="P5541_04660"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRV71304.1"
                     /translation="MNISGPDVNIGRESFDAVLFDLDGVVTRTAEVHAASWKQLFDGY
                     LQQISRDSEWKPFDIEHDYRRYVDGMPRYKGVKSFLASRNIDLPYGSPADPPERETIC
                     GLGNRKNLFFNRQIEKDGVSVYGASLDLIRLLRKKGFKTAVVTSSKNCVTILKAANIE
                     NLFDVRVDGIHIANEKLHGKPEPDIFLKAAKALGTTPGRSVVFEDAESGVSAGKKGKF
                     GLVIGVDRTGNSDRLLEKGAHRVVTDLSQITVDGDRPFPALPVATLPSAMESFHEISA
                     RLKERKIFVALDYDGTLTPIVDRPEDAIISREMRRKVNDLAGVCTLVVISGRDLKDVR
                     DLVDIDSLIYAGSHGFDISGPQGQNMSFRQGDEFLPLLNKAEKGIRNLLENIPGTSVE
                     RKKYSIAVHYRLAAEKDVPRVETIVDDSLAQHEGLVKGSGKKVFELRPGIDWDKGKAL
                     LWIMNALEFDPQTTAPVYIGDDTTDEDAFAVIGEHGTGIVVSEGKAGESTAARFTLAD
                     TEEVGVFLQRLTSWISEGENR"
     gene            20224..21456
                     /locus_tag="P5541_04665"
                     /pseudo
     CDS             20224..21456
                     /locus_tag="P5541_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            complement(21355..22215)
                     /locus_tag="P5541_04670"
     CDS             complement(21355..22215)
                     /locus_tag="P5541_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV71305.1"
                     /translation="MAHLANQLYGEPWRDFLLIGLTGTKGKSTTVYFLRSIFDGPEED
                     AAEKRFGYLSSIETYDGKVRFESHLTTPEALVLAEHFDHVRESGLCCMAMEVSSQALK
                     YDRTLGVEFDIGCFLNFGSDHIGGREHTDVEDYFSSKLKFFEQCKTVCLNVESDGLDR
                     IIAAVAASRLCEKRILYSPSGKAEWNGLPVDFLASDVHKEDRETVFRIHRRCGAGSSI
                     KAFEDLGEFRLTLPGLFNVDNALVATVVALALGSPLEQIKRGLYGAKVAGIFRGVSGR
                     IEAPVPVHQE"
     gene            complement(22491..23726)
                     /locus_tag="P5541_04675"
     CDS             complement(22491..23726)
                     /locus_tag="P5541_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71306.1"
                     /translation="MYKKCGISANGKSFIPLLFAGDINVYSMARAFHEAYGVCSKAYG
                     KYFTGPCVNSKIIDYTADPKAEDPVRFLELVRAFASAHPQETILLIGCGDSYVELASL
                     YKGSYPANVVAPYIDVDLMRTLIHKERFYDFCKRSGVDYPDTYVHRQEQGFHFTLPFD
                     GPFIVKPSSGIEYWAHPFHTQKKVYKVDTTEQVCSILAEIYGSGYDDSVIIQNFIPGD
                     DSHMRVLTGYSDRSGRVVMMCLGHVLLEEHTPHGIGNHAVILTESDRELVERYRILLD
                     DMKYEGFFNFDIKYDCRDDRYKAFEINTRQGRSNYYVTASGANVARYLVEDHLDHAVL
                     PYRCVDADSLWLVVPRKVAFEYVKEEKYRTRMKQLIRVDRWVNPLYYPADNGLRKRVA
                     LAKNQLGHFIKFKKYLEKQ"
     gene            complement(23719..>23971)
                     /locus_tag="P5541_04680"
     CDS             complement(23719..>23971)
                     /locus_tag="P5541_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71307.1"
                     /translation="ILSASMEAAAQPIHPLLYLLSIGTVFLGIYMTASHACFYDLVNG
                     NLQVRRTDELHTLAKEFSSPDPNATEEIPEETVGEDHDV"
BASE COUNT         5722 a         6902 c         5908 g         5439 t
ORIGIN      
        1 ctggcgttta tcttcgcccg ctccttctcc tgcctatata gctttgtcaa ggtgcttttt
       61 gcctgccccg gttttacggg gcgaaggcta tgataccgtg gctgacccat tgtgtcaaca
      121 aatacaggag gtaatcccta taataaaata acaacacccg gatatctttt acttactttt
      181 agattttttc agagctcctg gaggtcaaaa ggccacctct atcaagtcta cacctgtcaa
      241 attaaagccc cgaggaaaaa aggagtttac tgatcatcgt caacctttat gtcaagtatg
      301 ccttcctcta tctccagttc cgctttctgc agaagatcat caccgtcatc atctgaaata
      361 tcatcctgag ggacagaaaa accaaggccc tcggaggctt ttgccttgac ttccgagaca
      421 acctcgttaa gcagttccgg atgttccacc aggtatgtcc ctacactctc cttgccctga
      481 ccgagagtct cacccttgta ggcgatccag gatcctttgc gcttgagaac atcatgatca
      541 agtgctatgt ctatcagaga aacctccatg gggatcccct tgccgtagat cagagaggca
      601 tgggcggttc tgaagggtgg agcctgcttg ttctttacga cctttatcca tagttcatgg
      661 cctattgcat cgtcgccttt tgatatggat ttgcccctct tgacctcgat cctgagggaa
      721 ctgtagaact tgagagccct ccctccggtc gtggtctcct ggggacctcc gtaaccggtg
      781 gtagatatct ttgccctgag ctggttgata aaaaggacga cacagttgct tttggagatt
      841 gccgatgtga gccttcttaa cgcataggac atcagccttg cctgcagtcc gacctggctg
      901 tccccgatct ttccgtctat ctcggcctga ggagtcaggg ctgcaacaga atcgactacg
      961 atcacgtcga cggctccgct cctgacaagg gtctcgagta tgtaaagggc ctgctctccg
     1021 ctgtccggct gggcgacgta gagatcctcg atcttaactc cgagggaata cgcaaggcgg
     1081 ggatcaagag catgttcggc atcaataaag gccgcgatgc ctccctgttt ttgtgcttcg
     1141 gcaagagcgt gaagagcgag agttgtcttt cctccccctt cggggccaaa tatctcgact
     1201 atccttcccc tgggatatcc cccgatcccc agggcgatat cgagaggcaa cactccggag
     1261 ggtatcactt cagccacggg tccggcccct tctccaaggc acattatggc accctctcca
     1321 aatttgctgc gaatatcgct taaagcctgg cccagaacat cctccctggt catggaaccc
     1381 ttgcgtttcg ccattattta tcgcacctcc tggtcatttg tgaatttaaa taccgaaatg
     1441 ggtgtgtact ttgcaccctg gggaaaaagt tcgcttctca tgagcgttac agagcggacg
     1501 gtccactctc cccaggtatc gcttctggaa ttaagttcgc tgaaaacccc gatgggtaaa
     1561 ctggccgtcg gagggaccct cgcaagggtc acgtggggat gaaagggtct tctctccgga
     1621 tcaagaccag ctgctgtcgc ggccctttcg accacggacg cgaccctctg gacctggtcc
     1681 tcttctcctt caataccagc ccatagaacc tttggctgac gtatccccgg aaacgcccct
     1741 attccggaaa gttcaagcct gaagggtctg agcctcttga aggcaaaacc gtcctcaagg
     1801 gcattctgga ttttgcagac tgttttaaaa ggaatttctc cgcaaaattt cagagtcaag
     1861 tgaaaggccc cgtttctgat ccatttaagg ctcggggcaa cggtctccat ctcggccacc
     1921 cagtttccca ggttttttat tatttgatct ccaggttcaa tacacacgaa acagcggatc
     1981 atgtcatcct tcagacggat cacccccgac catatcgagc atggtttcaa caaggagatc
     2041 tgcggcacac ctgcgcacat cggctctctg gccacccaaa atcctgtgaa agaccttttc
     2101 ccctccggga aaagttacgc agaaccatac cagccccacg ggcttaatct tgcttccccc
     2161 tccagggcct gcaaccccgg ttatggctcc tgcaacatca gccccgaaaa gttttattgc
     2221 accccgggcc atcgcaaggg cacactcccc gctcacggct ccgaatcttt cgataatgcc
     2281 gggatccacc ccaagcaccc tgatctttgc ctcattggag tagcatacag caccgccgac
     2341 gaaagccctg gaggagcctt ccgactccgt cagcaaagcc ccggccagac ctcctgtaca
     2401 ggactcggca acacatatct tccatcccct gatacaggct tcggaaacaa gagcagcccc
     2461 ggatgttatt tccatgccct ggttccgggt tattttgtcc atcaaggccc ctccgctgtc
     2521 aggcgagtat cctcgaaaga ccccctgaaa acaaaagcca ggaggttccc ctgagaatca
     2581 ggttcgccat aatgccggcc actatatcgt ccgcgattat tcctattccg cccggaagct
     2641 tttcggcatt tctcacggga aatggcttga tgatgtcgag aatgcgaaag agaccgaagg
     2701 caggtacata gtatccggga gcaagaccaa tgagggccac ccacattcca gccacctcat
     2761 cgataacgat ctccccggga tcatctttac ctgaaaaggc cgcatacttg ccggaagcta
     2821 tcgtcccgac gactatgacc gaaagaataa gcaagggaga aacaggaata aaaaaggcgg
     2881 caacgactgc cgcaatggaa gccacggtcc ctggcatggg ggatatatac cccatgccaa
     2941 agcaagtact gacgggaata taccacttac ctgggttcaa acgatgacgc cacttctccg
     3001 ataaggtcat gctccatagc ctccgttatg cgcacatgca caaaatctcc aggctccggc
     3061 aaagggccgg accggatctc tacgactccg tcaacctccg gagcttccct gtagcttcgt
     3121 ccttcggcaa agccttcgga tgagtcacca gtttctacga ggaccttcag ggtccgaccc
     3181 tcgaaacgcc tctgccgttc cagagaaaca tcctcctgaa gcagggtgat cctgtcgacc
     3241 ctttcctgtt tgatatcttc gggaacctgc ccatccatca acgcggcggg ggttccctcc
     3301 tcagggtaat agggaaaaac tcctaccctg tctatctctg cctcggaaat gaaatcgagg
     3361 agcgaattga attctttctc cgtctccccg ggatagccga ccataaaggt cgtcctgagg
     3421 gcaaaatccg ggtcggccga cctcgccagg ctgaagatac tgttgatctt ttttctgtcg
     3481 agtcccccac ggttcatgga attgaggatc ctctcttcta tatgctgtac cggaacatca
     3541 agatagttca ggaaaagatc gctcgaggca actttttcga ccagtttttc gtcgacacgg
     3601 gcaggatgaa ggtagagaag acgaaaccag agatcttccc cccggaaatg ttcttccagg
     3661 ctttctatga ggccgcaaag cgaaaaggac ggggaaatgt catttccgta agaggtcaga
     3721 tcctgtccga caaggcatat ctcccttgct ccggcaagaa ccagcctctc agcattttca
     3781 attatcactg aaaggggtcg gctcgcaagg ggccccctta tcatgggtat cgtacagtaa
     3841 ctgcaacgat tgttgcaccc ctcgcttatc ttgaggtatc tgctgaaccg gttatatcca
     3901 ggaaggacca attggctttc cgtagaggta tgccctccca gaaacctggc aagagattcc
     3961 cattcaccgg cccttgccca aaagtcgact gattcgagtt cggctcctag ttctttttcg
     4021 taccggttaa caagacaccc gacaacggca agaaacctga ttttccccga ttttttgaga
     4081 atttcaagtt ccaggatcgc gtcaacactc tcctctatgg ccggggctat aaaaccgcac
     4141 gtattgacga tcgcgccgtc agcacatgat gaatccgtta ccagaacatg ccctccggat
     4201 tccagaatcc ctgccaggat ctcgctgtca acctgatttt tggcgcagcc aaggctaagg
     4261 agaaatatat tcatcccctt gaaactactg ccttttcatc gttccgtcgg ggagaaagtc
     4321 tatggtgacc acctctccgg atctgcctat atcccggata tcttttcctc cccagacaag
     4381 ggtaactgca cccgcattgc cgaaccgcac cgatgtcttc acatcaacaa cggtctccgt
     4441 aattcctccc ctgttcaggg tcatctgtgt agtttttccg tcggccctgg ttattcttat
     4501 ccagcagttt ccgtttgttt ttatgacgag ggaacccttg acaaccggtg attcttccac
     4561 ggtctcgaca atttcctgcc cggggatcca cgaggtgtca agaacgggtt cctcctccgt
     4621 gactgaaacc ccatcggttt caccggcgat ggcctccgca aggccggtct cctgggagac
     4681 atcagccgaa acgggtatct cctccggtga aagaacctcc tggctcttgc tgatatcggg
     4741 aacagatgcc atctgcgcct tcatggcatc tctctgctgc cagatcatgt aaagagatat
     4801 tcccgcagca aaaagcagaa aaccgaagat ccagatcctg gaacttttct ggaacccttt
     4861 ctggacaggc atgtactgaa ccgcggcctt tttccctttt gcggggacct gccctgagat
     4921 ggccagttcg tattcaggcc aaagatcaag ggaatcggtc acttccaggt aagtccgaat
     4981 aaaacccctg ctgtaaacat ctccgggaag atttccaaga gaaccgttct ctatggcttc
     5041 aaggaactgt ctgcgtatct tggtctttat tgagacatcc gaaagggaca gacccttttt
     5101 ctttcgtctt tcggaaactt caattcccag ttcttcagga gttcttcgaa tatccgtcac
     5161 agcctgtccc ctccagggct ttcgcccgca tctcccttat gaccccagcg gggtcggggc
     5221 tattgaatat agcacttccc gccacaagca catcacagcc ggcagaagaa acaagtgccg
     5281 aattttccgg tcccagtcct ccgtcgacct ccacaagata ctcataacca taagcctctc
     5341 gccagcagca caggagtctg actttctcaa ggacggcgga tacaaatttc tgacccccaa
     5401 aacccgggtt gacggacatg acgagcacca ggccaaccag gggaaggacc ggctttaaaa
     5461 gttccacggg ggtccccgga ttcagtgtta cgccgggggt acatccaaga tccactatcc
     5521 tctgcagggt cctgtgaagg tgaaccgtcg cttcctggtg gaccgtaatg atcgtggagc
     5581 ctgcagcagc aaagggctcg ataaagttct ccggtttttc gaccataaga tgaacgtcaa
     5641 ggacagcctc cggccatttt ttgcggcaag cctctacaag cgagggaccg taggaaagat
     5701 tgggcacgaa gtgtccatcc atgatatcca ggtggatcca gtcatgttct ccctctagtc
     5761 ttttaatgga ccctgctatg tcaagagggt cagcggaaag gatcgacggg gctatctttc
     5821 ggcccgggca accttttact ggtagcgatc ctgccatacg aactctcctc caagatatat
     5881 cgtcaccgat gactgcccct ggtagggcgc atcgagagat acatattcac ctgtacgaac
     5941 ctgcctgtcc ataagtaccc tggaaccgtt cttgtcgaca atctcgatcc tgagtgccat
     6001 tggttccgat aatggaggca cctggtacct tatacgggcg atcttgtccc ctggggtggg
     6061 agactgggca ggaggctcct ggcccaccga aggagcctga ggctgggtcg gcggactgtc
     6121 cgctgccggt gccgcaggaa cccctcccgg ggtcgcgggg ctggtatctc cggctgccgg
     6181 cgtttcctcc gtagttggcg cctctggttc ctcgggtttt ttacccgtcg cgaccacaag
     6241 gtttacgact ccacccggga gaagggtctt gcccggcttg ggttccatgt tcatcaccat
     6301 accggcaggg gtcatatagg tatactcata ccgcaccgat ccaaccttga gcccgctttc
     6361 gacaacgatc tggcgcgcga tcttttcgct cctctcgagc acatccggga cggtcacctt
     6421 tcccccgacg cgaggaggac ccaggctgac cagcaggtcg attcttcttc ccggcgggac
     6481 cattgcaggg gaagcggggc tctgggccac aattattcca gcaggacgat tatcgctgtg
     6541 gatcctgacg atatccccca catcgaaacc cgactcctga agcctggtca ctgcctgtcc
     6601 atattccatt ccccttacat caggcagagg ctgtctgtct cctcccctgc ttaccttcag
     6661 tattacgatc ttccctttct tgacacgcgt tccgggttca ggccactgtg acacaacctc
     6721 acctgagggc tgaagagact cgaccttgtc gatcctcaca agaagcccca ttttttctgt
     6781 catgcttact gcgtcaatca ccggcattcc cgaaagaccc ggaaccgcaa catccctgcc
     6841 cccgaaaaat atggtgtaga aggcgacgat tcctgagccc gcaatgacca gaacaagaac
     6901 tagtaacgtt accttgaaca acctctgcat gggtaatccc cctaaacgcg tttgttaagt
     6961 acggcaaggt aaaatccgtc cagccagggc agcgtgggga aaatataatt cccccatggt
     7021 ctgccgcgac tgaaactatg ataggtgttg tccggaggca tttcaacaac atcccccctg
     7081 ctttccagaa cttttgcgac aacctgttcg ttctcctgcc tgaaaaggct gcatgtggaa
     7141 tagatcacgc ttccgccggg cgggataata tcaagggcct tcgagagaag agcacactga
     7201 agggtgtgca tatctccgat cttgtcaggg atcagcctca tcttgccctc aggatgcctc
     7261 gaccaggtcc cgcttccgga acagggagca tccagaaaaa ctgtttttgt ctctcccgaa
     7321 gggaaaaggg tgagcgcatc tccgcttttt gcgatcatgc gatccgaaga gataccgagc
     7381 ctcctggcct cagataatgc ggctctcgtt tttgctggag aaacatccca gcactcaaga
     7441 acactgcccg gcaaagcgtc aaggatctgt aaagccttta ctccacgacc agaacacata
     7501 tcaagcaaag cgccacccga atggatcgaa gagaccaccc tccctatgat catggatgat
     7561 tcggactggg gggaaaaaag accctccgcg taaccgggaa gagacggcgg atgagcactc
     7621 gaagataatc tgaggctctc ctcgagaagt ggggacctcc aggacctgta accaccttcg
     7681 gcaaggcggg agatggtatt ttcgatatcg gcacggggcg aaagcctcag agacatcatc
     7741 gacttcatgg atgagaaacg gacaagatcc ctggccctgt ctttacccca tgattcggac
     7801 cacattgccg ccgcccagga tggaacacct ccggccagag cgttatcttt aagagacctg
     7861 cttgtggaaa attttttaag aatggcgggg ccctcttcca cggcccttct caaaacagca
     7921 ttgaccatcc cggactccct ctggtttccg ctttttttta cccattccac catcgcgttg
     7981 accagaacct tcggcaaaaa gtgccttagc tccgaaacac cggcgatccc caggacaaga
     8041 gcgtcaccag cagcctgcga catggttcca aggggccttc ctgtcatttg ccttgcaata
     8101 tgcttccaca gaagcgctct tctcaaggca acgaagacaa gtgtcgttgc caggatcctc
     8161 tcgctttcgg gaagggaaga agacctcttg cggatggcct cgctcgcaaa acttcccccc
     8221 tggacatctc gccagacaag aagtgccgct tccagacctc tcattgataa accacctcgg
     8281 tatctggagc ccttcctttg accctggtat ggacatatac ctccatcacc gggacgacag
     8341 acctcacggg gcgcagatag acaccggata tctccagtga ggaaaaatca ggatcagtag
     8401 tgatgtggcg ctgtacccag gggctagcca ccggtccgat ggcgtaggcg gcaacgactg
     8461 aaagaattac tgccagaatt gagatccttc cggcaatgag caaaagaagg agaaagatca
     8521 cggccgagat ggtgttgata acaaccaggg tagtaccgca ccttttatta acggtcaggg
     8581 cccgatctcc ggcagagatc ctttgcctgg cttcccgtgc ggcatcgaga acgtacgccg
     8641 gatcggcaat accacgaatg gcgaaaccat ccttaaagga ttccccggta agaagacgac
     8701 ctgctcccct ctcctcgata acgtttattg ttccatgttc gagagcatga ttctttctcg
     8761 tgagcggatc agaaattacc cgaacaaatt cccgaggccc tacgacaagc cacgcaaggg
     8821 atctcgccgc aaaaccgaga ggaaccagca ggaaaaggaa aagaaaaaag gcaagcaata
     8881 tcagcccaag ccatgggact atgaagatca taataagggc aagaaagatc agtggcatgg
     8941 tttattcgac tctcctctcc ttgccggatc gttcaaaata aagaacggcc aaaatggaac
     9001 gggggcggca aaagccaccc cccgatactg tccaggcagg acaatggccg aagaattgca
     9061 gatctttcct aacggtttct catcccgcgc agaagaaaaa gtctgacgat ctgaagcgcg
     9121 gacatcagcg tggccgcgac atatgttagg gctgcggcat tcaggacagc ccgagcccca
     9181 ccgatctcgt catccgcaag gagccccgaa ccagacagga tcgaaagcgc ccttttgctg
     9241 gcgtcgagtt ccaccggaag ggtgacaagg tgaaagacca gaactgcgga aaaaaagatt
     9301 atccctagat ccatcagcaa cggtgtacca aaaaggaacc cgatgaaaaa aagcggcagt
     9361 gccatggaag aaccgatacc ggcgacaggg acgaaggcat tccggagacg aagaggtaca
     9421 taccccctcg catcctggat cgcgtgtcca gcctcatggg cagccacacc tatagcggct
     9481 atactgcgac tgtctattac gctgtccgat aacctgagga cctttgacct gggatcgtaa
     9541 tggtccgtca gcgagccccg tatcctctcg acagatacat tctgcagtcc gtttgaatcc
     9601 aggatcgtcc ttacggcacc actggcggta actccccgcc ttgcgttaac ggaactgtac
     9661 ctgctgaaag cgcttttaac tttagactgc gcccacaccg ccaatatgag tgctggtaca
     9721 agcaacagaa aggtagggtc aaaaataaag gggaacattc tgcttcacct ccgtgctggt
     9781 ggtatcagcc aactgtacag actatttcac atgggcaatg ctacatcagt caaaccacaa
     9841 tgtcaataaa ggtcaaacac ccttgcccgt ttatttactg taaaaaccgg ctatcccttc
     9901 tgcgaccctt tcctgttcct ctggaagaag ttccgggaat atcggcaagg caagggtttc
     9961 ctccgaaagt gcctcggaga cagggaaatc gcccttgcga tagccaagga aagaaaaaca
    10021 gggctgcaga tgaagcgaaa ggggatagta aacccttgaa gttatacccg acctctggag
    10081 ttcttccttg aggttgtccc tgtccttgca tctgattacg tattggtgat aaatatgtct
    10141 gttcccgggg gcttccacgg gcggagttac aagttcccca agattatgct ccgcgaagag
    10201 agtgcggtag atctcagcga cgagacgcct ttcctcgttc catgtctcca ggtgtctgaa
    10261 cctcaccctg aggatcgccg cctgcaactc gtcaagcctg ctgttcagcc cgacctcgtc
    10321 gtgaaaatac tgcttccccg atccgtgcac acgaaggctt ttgatctttc cgccgacctc
    10381 ttctgacccg gcggtaacca ttccggcgtc accgtaggcg cccaggttct tggtcgggaa
    10441 aaaggagaaa caccctgcat caccgatcgt tccggcccga agtattttgc cgtcgatcat
    10501 cctggttgag ccaaatgcct gtgcacaatc ctccacaaga aaaataccgc gctcctttaa
    10561 ccctgcttca aaaacttcca tccgggacat ctggccaaag aggtgaacag ggataacggc
    10621 ccttgtacgt tccgtgatct tctccaggac cccctcgggg gagatgttgt acgtgtcggg
    10681 ttcgacatct gcgaaaaccg gagtcgcacc gacccttgta atgcaactga cggtcgcgaa
    10741 aaaactgtag ggagtcgtta tgacctcgtc accgggacct atgcccaggg ccataagagc
    10801 aagaagaaga gaatccgtcc ctgaggcaca ccccacagcg aggggtacgc ccaggtagga
    10861 ttcgacctct ctttcaaaag catcaacctg gggtcccagg atgaaatgct gactttcgag
    10921 aacttctttt attgcctcca gtgtttcttc cctgacacgc tcccagtttc tggatagatc
    10981 aaatgacggc ataacaggac tgtccattaa acgctccccc ttacggtccg aaaatgattc
    11041 ttcctgaact ccacacagaa atattgtccc tggccgaagg gcgaccgtgg caacggatat
    11101 cctttttaca gcagttatca ggacggcttg ttcagcccta caagcgaaag cttcaggtgc
    11161 tctttcatga gttccactgc cttttccctg tcgcgatcct tgagagcctg ggctatggcc
    11221 ttatgttgat cgatgctcgg attatcgacg atatcataga acgggtcata gaatacgacg
    11281 taggcgttgg ttctggcaag gatgttttcc acaaactccg caaggacccg gtttccgctt
    11341 gcatcggcaa tgatcttgtg aaaattttcg ttgacttcga gatataactc aaggtttttc
    11401 tcctcgaagg ccttctcttc ctccaggatc gcgtcttcga gccttctgag gttcctttcg
    11461 cttatcctgg tcgcggccgt ggcgacggaa atgcactcca gttgttccct gacagcgtat
    11521 gtatcctcca tctccctttt ggtcggagaa gccagcctgg cgccgctgtt gggaataatt
    11581 agaacgagac cttccgaaga gagcctcctg agcgcttccc tgacgggagt cctgctaact
    11641 cccatctgaa cggctatgtt gacttccggc aaccgctgtc ccggtttcag ctgtttggtg
    11701 ataatcctgt gcctcagttc ctggtataca taatcggccg atgtagtgta tagtctgggt
    11761 tccatgatca ttttccctct cccctctctg acggagaaaa tagtattaca tgaatacatg
    11821 catgctatca tataccggat tcagtttcaa ttattttcat acatttaccg agcatatgaa
    11881 cgcgggggaa aaactctgcg ggaaaggtca taaaatgcgg ttttttgttc ttgttctttg
    11941 cctgggaacg ctggttatgt acggaagggc gatcgccggg ctctggagtt tcataatcgg
    12001 ggacccgaga ccctggatga tagtatcagg gctgatcggc ggtacccttt ccggggggat
    12061 cgccattctt ctatggaaga agaggctcag ggaactggaa tggataaggg aaccggaaga
    12121 aaccaccgaa gacggagaac ctttacactg acccggagag atccctgata ccttcgttca
    12181 caagccggga agcagcctcc ctcagggtat tgatatcgtg tcttttctct cttgcgcact
    12241 cctttgccgc gctttcgcag agcaaacatc tccggggatc tcttccgagt gattcaccgt
    12301 gaagggttcc ttccgggcca ataacatcaa tgtctatcac tgacccccaa ctccttgcct
    12361 ccaccttcat tgcgatcatt tttacctctc ggccatccgc tccgaccagg cccataagta
    12421 gcgcacaacc cgctccgtta tccagagtga caacgcaggg aaccacagcc cccgctgatt
    12481 ccagattccc gcggatcagt cggcctgtca tttcaaccag atccctgtcg ccatccagcc
    12541 ttttggggat acccgggatg ttaagcgata cctgcagcac cagaccgaac ctggagacca
    12601 cacgcctctg aagccaggct ctctcctctc tccctgcaag aagaaaatcc aggaggctca
    12661 ccgcattatc cccaaaccct ggaaagacgc tgcacagcct ctattatcat cgccggggaa
    12721 accctgctga ccgagattcg tacggcttcg atatccctgt tggggttaac aaaatgccgc
    12781 ccggggacaa ccgccacgtc cgcttcactg gctagacgcg cggcctcttc cccaccaaga
    12841 ccatgggtgc cgagccagag gtagatcccc ccctgtggct gttcgaatgt aaaacagggc
    12901 agcttttctc caatttcccg acatagtgtc gccatccttt cggacatcga ccggcgtgca
    12961 ttctccagcg aactttcaag gctgccgtcc tcgatgaatg cgtgaacgac attctggacc
    13021 agcgaggaag tcgagccgga agtcctttcc atgatcttca ggaattcgtc gcgtatatca
    13081 tctggaagca ggacatagcc gatcctcatc cccggaaaaa gcaactgact gaaagaacca
    13141 atataaataa ccctgttctc cgtatctatc gatttaagag ccggaacact ggaatcgcaa
    13201 aaacgaagtt caccgtaggc atcgtcctcg acgaccagaa aacctctctt cgaagccgaa
    13261 gaaagaacct ccttacgaat atcaatggga agtgttcttc cggtggggtt gtgaaaactg
    13321 ggtacaaggt agaggacctc tcctctctcg atgcgggaaa gagattccac gaatgcatcc
    13381 tgatccatag gaaccgaagt gacatcaatc cccgaacctg ccgccatacg cacggcctcc
    13441 gggtatgtca attcctctat ccagagtctt cttacaccct tcaaagccat cgcgtcaatg
    13501 gtcagggaca gggcgtgcct cgcgccgcta gttacaatgg cctcgtctct tttggcgggg
    13561 ataccccttc gggcagcgtg acgcaccagg gccttcctca gcccggcact accctcaaga
    13621 ggagtatcaa acataaatcc gggcccgata aaggaaatgt tttcccttgt tatggatgct
    13681 actctctccc agggaaccag atctcccgac ggcagccccg aagccagatc ccatatcgga
    13741 cccgtgtcgg aaggggcaat aagggattgg gccctcttga gacttacgaa agaacccctt
    13801 cttccctttc tttcgatgaa accatcatct tcaaggaggt cgtatgccat gacggctgta
    13861 gtcctgctta tgccgagact ctgcgccagc tccctgctac caggcaacct gtcgccgcag
    13921 gaaagggtgc cgttctgcac catcctcctg agatgcccgg caatctgcct gtacagaggg
    13981 gtcatgcctc ccggttcaag gggtatttca atcattgtgc ttctcctttt tcaggggatc
    14041 tacacaatga tacacccctg cggtctaaag acgtccggaa agatccttca gcgtctccag
    14101 gacggcattc atctcctctt ccagacctac agcaaccctg acccaggttc cggaatactc
    14161 tgaaagagat cccatttccg ggaaacggac cttgatccct ctcttttcga aggcagccct
    14221 tgcggggtcg acgggaccct tggaataaag ggttacaaag ttgccgttcg ctggaagcgc
    14281 agtccatcca ggtatccggt tggtctcttc gataaaacgg tctcttgtaa aggctatgcc
    14341 cttcactctc gcctgcatcc attcggagta tcccagggcc accctcgcgg cctcctgcga
    14401 aaaaacactt acattatagg gagggcggat cttttcgatc ctctctctaa ggcagggcct
    14461 tgaaacaccc catcccaccc ttaaaccggc aagaccccac gccttggaaa aggttctaag
    14521 cacaaaaacg ttcgggggaa gtttttccgg ctccgaaagg aaggaaccac cgcaaaaatc
    14581 cacgtaggcc tcgtctacag caagaacgcc ttttgaaagt ccggcaactc tggaaagcat
    14641 ttctgttgaa accgacagcc ccgtcgggtt atttggcctg tcgataagta tcagatcggg
    14701 tgaggccgcc gaaattgcgt cggcaagttt ttcttcgtcg agaaaaactc tgctcccgtc
    14761 aaaacaaagc ggcacagacc tggtcctggt cccgagagcc cgggaaatgt gctcgtaacc
    14821 ggagaaggat ggagaaaggg tcatgaggca tccattatcg gggacaaagg ccatgatcag
    14881 ataaagcagt atctcgtctc ctccgttgcc aatgctgatc cagtcagcgg gaaacccgca
    14941 gtgaacagaa agagcagacc tcagggcctc cgcttcctgg tccggatacc tgttgaattc
    15001 cagagaagaa agagaatgac gcaattcctc tataaaacca ggaggtggat cgaagggatt
    15061 ctcgttcttg tccagacgca cctccgaaaa ggtcgcctgg ggggaaaatg gggaacatgc
    15121 agcaactctc actatattac ctccctgttc ctttgccgtg gtgaacggct ccatcaaaac
    15181 acgattggca gtttatcagg ggaacagggg ggttgtcaat ggggcaaagc agcatggggg
    15241 caaagcagcg tgaatcggga atcgtgaatc gtgaatcgaa agggccaaag gcatacccag
    15301 atctccgccg gcgggcatat ggtgcggaac gtccgcctgc tggcggattc ctcttcgctg
    15361 cgctcagaat gacaggcttt tgatgtcatt ctgaggacca acgggacgaa gaatctggtt
    15421 taggtgccgt gaatcgtaaa tcgaaaattc aaaacacaac gcaacccgga ttcccgccgg
    15481 taggcaaagg gtgcggaacg tccgcctgct ggcggattcc gcttcgctac gctcagaatg
    15541 acaagcgttt tgctcttaag gtttttccgt tcaccgttta ccgtttacgc acttgaattt
    15601 ggtttttccg attcccgact cctgagaaga gaacagatcc attggtcctg gaacttgccg
    15661 cgggcccatt cgtactcctc aagaagcccc tcaaccttaa aaccgaggct cttcaacaaa
    15721 ctcaacgatc tttcgttccc gtctgtcacc agagcctcga ccctgttaag atccatctct
    15781 ttgaatccaa actccaggat agccgacatg gcttccttca tgtatccctt tccccaaaaa
    15841 tccttgccca gttcataccc cgtctctgcg cgaaaatgct ccttcgccca attgtgataa
    15901 ccgcaagtcc ccataaccgt gccgtcagaa gccagggtta ccgtccatct gacccccgta
    15961 ccggtttcgt aaagcccatc aagcagttcg atcatggcaa tggcctcctc gagagtctca
    16021 aagggatcca gaaccatgaa ctccaggaca acggggtcgg accagatatt atggagcatc
    16081 ggtgcatcat ctctggacaa ctgcctgagg accagcctct tagtcgttat cacgggaaaa
    16141 agactgttgt cacgggagaa aacagctttg ccatctgagc atttatcgat catgaaagaa
    16201 attattcctt ccaggagcct tcaggaatat ccacctggcc acatttgctg tccagccaaa
    16261 acttcgtggt agcggcaccc aggttattgc ggatcgcttc atcaataaag accagcgcct
    16321 tccgcagatc ttcatccctt tcaggggcca gactctccat gaaaagaagg gcccgcttcg
    16381 tctccggggt cagtttggtc ctgtcggcct cacgccagtt ccagcagcgg caaatggccc
    16441 ccgcgtcgtc caggtaaact atctccccct cacggggggg gtcgttctct tccgacccaa
    16501 gcggcaggaa tggctcacct ccccttgcct gaatcaggcg aacatcacca atcaaggcgt
    16561 caagatcctc cgccccgcag gggaaaagga acttcagcga ggttgcattg tatagatcca
    16621 ccagcgggtt gatcgagggc gggcaacttc ccttcaccgc acgttttgca agggcctcca
    16681 cggaggagcg ataacctttg ggggccccaa aggcacggaa cgcctccctc cagggagcaa
    16741 aaaacgaact ctcggcaaaa ggcgcatctc ccagccttgt agccgcttcc cggcatgcct
    16801 gctcaagcat ccctgttccg tcccacggga tgccgttgtc catgttcctt ataaccagac
    16861 cgcagacacg agcctgtggg aatagttcaa aaaccttgtc gaataccaca aatttccgca
    16921 ccgtcgttca catcccccga attagaaatc caggcatcat cacctgaaac tgtttttagt
    16981 atatgtgttt ttctcttgat aaatagagga ttggcagaaa aacatgtttc gtggttttgc
    17041 ggtttcgcgg tttcgtgatt tcgtggtttc gtggttttgc ggtttagtga ttcgtggttc
    17101 cttcttttca attcacgccc tttcccttgc ctctcccgtt ctccgtttac cgtttaccgt
    17161 ttaccgtcga ccgttcacgc ctttgatcct gctgtcatcc tgagcaaagc gaagcggaat
    17221 ccgcccaccg gcggagatct ggtttaggtg ccgtgaatcg taaatcgtga atcgtgaacc
    17281 gtgaatcgga aaggcaagga ggctggaaac tggaaactgg aagggccaag ggccagagcg
    17341 tgaggggagg ctttaccgaa ggctcaggag ccggtgagtt tcgcagccgt cgcggagggg
    17401 cagggaaagc acacctgccg ggggtttttc ccggcactgt gcgatcgtcc cgcagcaggc
    17461 ggagaaacga acgctttcag gcgagttgcc tctcgggaaa gacctcccga tgggcaagat
    17521 cagattcttc ctcccgttgt tcgtcagaat gacagacctc cgattcacaa ttcacgattg
    17581 ttcggttcca gatccacaag tcacgtccct gatttagggg ccctaggcac gaaccattgc
    17641 agtcccgcat ttgggacaac gcacactggt acaggggctt cctgaacgat ggggagccct
    17701 ctctccgcag gacgggcaga cacattcact acctggccct gcacccaacc ccgttccact
    17761 ctgattcctg ttacccatac ctcttcctgt tttggctccc tgccctcctg gtcctgttcc
    17821 gtctcctctg ggcataatta cccctcctta atatacctta atcttttaca aatattatac
    17881 cattgtctgc acattgctgt tttttcaaac ttttttctgc tacaaaaata aaaatttttt
    17941 tccattcaga caagcaacgc ctcaactcgg aacgctgctc ccaggccaaa cacttcgacc
    18001 aggttaaagg aaggaagaca gtttatctcc cgccacgcag tgccgggcaa accctcgctt
    18061 tcgctttcgc cctcgccctc gccctgcctc ttccgttcac cgttcaccgt tcacgccctt
    18121 gatccttctg tcatcctgag cgaagcggca tctcccagca ggcgactttc cgcaccatat
    18181 gcccaccggc ggggatctgg ctctgacccg gacaagacct cgaacccggg gctgaacata
    18241 cactcttttg gccccccatg gttcctggca cttcttggga acctgggagt ggcttaccgg
    18301 gaggcctggt cactctctgt gacgggggaa tccttcgatt tcggtgacga actgacccag
    18361 gcctgccttg gcagctgcaa aaaggcccag gccgctttca gggaaaagta tcgctgatcc
    18421 ggcataatcg ccagggagac cgtgttgcca ggcctccctg gcgacccttt ctggcacgag
    18481 ccctttacta ataagaacaa agacaagaga ataggagaga cacaatgcgg cgcgaatgca
    18541 agatctggag aaccttcagc tatttttgag gaggacgttc aagcaaaaac cgacccgccc
    18601 ctggctgaat atcgcgcctg gaaagggagg gaaaagaatg aatatttccg gacccgatgt
    18661 aaacatcggg agagaatcct ttgacgcggt cctgttcgac cttgacggag ttgtaacaag
    18721 gactgccgaa gtacatgccg cttcatggaa acaactcttc gatggatacc tccaacaaat
    18781 cagcagggac agcgaatgga aacccttcga catagagcat gactacagac gttatgttga
    18841 cggcatgccc cgatataaag gcgtgaaaag tttcctggcg tccagaaata tcgatctgcc
    18901 ctacggcagc ccggccgacc ctccggaaag ggagactata tgcggcctcg gaaacagaaa
    18961 aaacctgttt tttaaccggc agatcgaaaa ggacggtgta tctgtctatg gtgcgtcttt
    19021 ggaccttatc cgtttgcttc ggaaaaaagg gttcaaaacc gctgtagtta cttccagcaa
    19081 gaactgtgtc accatcctca aggcagcaaa tatagaaaac cttttcgacg tcagagtgga
    19141 cgggatccat atcgcaaatg aaaaactcca cggcaaaccg gaacctgaca tattcctcaa
    19201 ggcagcaaaa gcactgggca caacaccagg acgatctgtt gtgtttgaag acgccgaatc
    19261 cggagtttct gccgggaaaa agggaaaatt cggcctggtc atcggggttg acaggacggg
    19321 gaacagtgac cggctcctcg aaaagggagc acaccgggta gtaaccgacc tgtcgcagat
    19381 aaccgttgac ggggatcgcc ctttccctgc cctgcccgtt gcgacgctcc cttcggccat
    19441 ggaatcattc catgaaatat cagcccgcct caaggaaaga aagatctttg tggccctgga
    19501 ctatgacgga acgcttaccc ccatagttga cagaccggaa gatgcgatta tttccagaga
    19561 aatgcgtcgt aaggtcaatg atcttgccgg ggtctgcaca ctggtcgtca taagcggcag
    19621 ggacctgaag gacgtaagag acctggtaga catagacagc ctgatctacg ccggcagcca
    19681 cggcttcgac atttcgggac cacaggggca aaacatgtca ttccggcagg gggacgaatt
    19741 tctcccgtta ctgaacaaag cggaaaaagg tatccgcaac cttctggaga atatccccgg
    19801 cacatccgtt gaaaggaaga aatactccat cgccgtccac tacaggcttg ccgcggagaa
    19861 agatgtaccc cgtgttgaaa ccatcgtcga tgattccctg gcacaacacg aaggtcttgt
    19921 taaaggttca ggcaaaaaag tctttgaact tcgacccggt atcgactggg acaagggcaa
    19981 ggctctcctc tggatcatga acgctctgga attcgacccg cagactacag caccggttta
    20041 cataggggat gacacaacgg acgaggatgc attcgcggta attggcgaac acggcaccgg
    20101 catcgtggtg agcgaaggca aggcaggtga aagcactgcc gcgcgtttca ccctagcgga
    20161 cacggaagag gtcggggtct tccttcaaag actcaccagt tggatcagcg aaggagaaaa
    20221 cagatgagct catggagatt tgcatacgac ggcttcgaac cggataaaga aggtctgagg
    20281 gaagccctgt gcaccctggg taacggctac ttcgccacca ggggggcatt tgcgggatcc
    20341 gaagcgaacg atacccatta cccgggcacc taccttgccg gaggctacaa ccgtctcaaa
    20401 accgggatat ccggccgaac gatagagaac gaagacctgg taaactttcc caactggacc
    20461 cttctcaaat tccgtttccc cggtgaagac tggtccagtc tcacggatgt tgccatactc
    20521 cattatcggc aggaacttga catgcgcaaa gggatacttt cccgggacat ccgatttgag
    20581 gacagccagg ggcggatcac aaagtttgaa agccgcagga tcgtccacat ggcctatccc
    20641 cacctggctg cccaggagac aaccattacc gcagaaaact ggtccggcac ggtggaattt
    20701 ttgtccgccc tcgatggtac ggtaatcaac tacggggtcg agcgctacag ggacctcaac
    20761 atggttcacc ttgagcccat ggaagaaaag gaacttgacg aagaaaggat ccttctcaag
    20821 gtccggacca accagtctcg actggagatc gccgaagcgg cacgcaccct ggtctttgtg
    20881 gatggcgtcc agcaaacaac agaaagaacc atcatccgga agcccgggtt tatcggccag
    20941 cttttcggtg taagccttaa gaagggcgga agagcacgcc tggaaaagac ggtaagcctc
    21001 tttacctcga gggaccgtgc catttcagaa tgcggcctgg aagcatccag cgccttatcc
    21061 ggtgaaacta ccttcgaaca tatccttgaa agccacgtat catcctggga tcgcctctgg
    21121 cgccgtttcg aaatggattt ttaccccggc gacaaaaaca ggggtgaccg cacagcccgc
    21181 atcctgcacc tctacaccct tcacctgctt cagacggcat ccatgcacac catggacctg
    21241 gatgtcggca tacccagcag gggttggcac ggcgaagcct atcgcggcca catcttctgg
    21301 gatgagatct atatctttcc gctgctcaac ctccggatgc cggaaatcac ccgttcactc
    21361 ctgatgtaca ggtaccggcg cctcgatgcg gccagaaacg ccgcgaaaaa tgccggccac
    21421 cttggcgcca tacaagcctc ttttgatctg ttccagcggc gatccgagcg ccaaggcgac
    21481 aactgtcgcc accagggcgt tatccacgtt gaacagtccc ggcagcgtca acctgaattc
    21541 tcccagatct tcaaaggcct tgatcgaaga tccggcgccg catcgcctgt ggatacggaa
    21601 cacggtttcc cggtcttctt tgtgcacatc ggacgccaga aaatccacag gcagtccgtt
    21661 ccactccgct ttgccggaag gcgagtacag gatgcgtttt tcgcacagcc gagacgctgc
    21721 cactgcggcg atgatcctgt ccagaccatc agactccacg ttcagacaga cggttttgca
    21781 ttgttcgaaa aacttcagtt ttgaggaaaa ataatcttca acgtccgtgt gttcacgtcc
    21841 gccgatgtgg tcggagccaa aattaagaaa acatccgata tcgaattcca caccgagcgt
    21901 ccggtcgtat ttcagcgcct gggaagaaac ttccatagcc atgcaacaga gcccgctttc
    21961 gcggacgtgg tcaaaatgct cggccagcac cagggcttcg ggagtggtca aatgggattc
    22021 aaaacgcact tttccgtcat acgtttcgat ggaggacaga taaccgaaac gcttttccgc
    22081 cgcatcttct tccggcccgt cgaaaatgct gcgcaaaaaa tagaccgtcg tcgattttcc
    22141 cttggtcccg gtcaacccga tcaggagaaa atcccgccag ggttctccgt acagctgatt
    22201 ggccagatgc gccatcgctt ttcgtatatc ggtcactacg attccggatc cttgatcgtg
    22261 ctcctgttct gcgatccatg caatggcgcc tctgtccagt gcatcggtga gatagccttc
    22321 acgaaatcct gcgcctttgc acacgaacaa cgatccgggg atcacgtctc tggagtcgta
    22381 ggtgatgtgg cacaccggtg ccgacagcaa ggcttcgtcg gacagcgaac gacggatcag
    22441 tgctctggaa cgcaatactt tttcatattc ggacagtgtg caaatccttt tcattgtttc
    22501 tccaaatact ttttaaattt gatgaaatgt cccagctggt tttttgcaag cgcaacccgt
    22561 tttcgcaagc cgttgtccgc cggataatac aaaggattca cccaccgatc cacccggatc
    22621 agctgtttca tccgcgtccg gtacttctcc tctttcacat actcaaacgc cactttgcgc
    22681 ggtacgacca gccaaagaga atccgcgtcg acgcatcgat agggcagcac ggcgtggtcc
    22741 aaatgatcct ccaccagata gcgggccaca ttggcaccgg aggcggttac gtagtaattg
    22801 ctccggcctt gccttgtatt gatttcgaat gctttgtagc gatcgtcccg acagtcgtac
    22861 ttgatgtcaa aattgaagaa cccctcgtac ttcatatcat ccagcaaaat acgatatcgc
    22921 tccaccagtt cgcgatcgct ttccgtaagg atcacggcgt ggtttccgat cccgtggggc
    22981 gtgtgctcct ccagcagtac gtgcccgagg cacatcatga ccacgcgccc gcttctgtcc
    23041 gaataccctg tcagcactct catgtgggaa tcgtcgccgg gaatgaaatt ctggatgatc
    23101 acggaatcat cgtagccgga accgtagatt tccgcaagga tgctgcagac ctgctccgtt
    23161 gtatcgactt tatatacttt tttctgggta tggaaaggat gcgcccagta ttcgatcccg
    23221 gaggaaggct tgacgataaa aggcccgtca aagggcaacg tgaaatgaaa gccctgctcc
    23281 tgccgatgga cataggtgtc cggataatcc acaccggacc gtttgcagaa atcgtaaaat
    23341 cgctctttgt ggatcaacgt ccgcatcaga tccacatcga tgtagggtgc caccacgttc
    23401 gcagggtagg atcccttata caagctcgcc agctccacat agctgtcccc gcatccgatg
    23461 agcaggatcg tctcttgggg atgggcggag gcaaaagccc gaacaagctc cagaaaccgt
    23521 accgggtcct cggccttcgg atctgcggtg tagtcgatga tcttgctgtt cacacagggc
    23581 ccggtgaagt attttccgta ggctttcgaa cagacgccgt aggcttcgtg gaaggccctt
    23641 gccatactgt agacgttgat atccccggca aacagcagcg gtatgaaaga ttttccgttg
    23701 gcggatatgc cgcacttctt atacatcgtg atcctctccg actgtttcct ccgggatctc
    23761 ttccgtcgca ttcgggtccg gcgaagagaa ttccttcgcc agggtatgca gttcatcggt
    23821 cctgcgtacc tgcagattcc cgtttaccaa atcgtagaaa cacgcatggg acgcagtcat
    23881 gtaaatgccg agaaatacgg ttccgatgct cagcaggtac aaaagaggat ggatcggctg
    23941 cgcagctgcc tccatggaag cggacaggat c
//
LOCUS       DAOTIQ010000024        23535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2534_length_23535_cov_7.748254, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000024 DAOTIQ010000000
VERSION     DAOTIQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 23535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23535
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_23535_cov_7.748254"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1233)
                     /locus_tag="P5541_04685"
     CDS             complement(256..1233)
                     /locus_tag="P5541_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRV71308.1"
                     /translation="MDILYLNRSQIESMRIPVKEVIEAVEEGFTLKGEGMVQLPRKPE
                     VHPREDCYIHAMPSFISGIADTAGIKWVSRYPSNPEKGLPLIMGLIILNDAETGVPLA
                     VMDAGWITAYRTGAATAITANKLAPKGASTLSIIGLGVQARTQLIALRETMKGLRNIR
                     VFDIIPDHSRKFLEEMQRKAPELEIEIFSDPESATRGSDIVVTCTPIRREPERFIPAE
                     WLKKDVLAISVDYDSAFEASVMTEASFFTTDDRAQYLWMKEGGAYFSEYPSEVLADMG
                     EICAGKMEAPQNGGRRAAVLIGLASHDLVTARVIYDKACRIGIGTILEF"
     gene            1397..2245
                     /locus_tag="P5541_04690"
     CDS             1397..2245
                     /locus_tag="P5541_04690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRV71309.1"
                     /translation="MREVHVSSIEEAVKNLFIKANTSIGQDVYNALVHALGKQEDSPT
                     GRDVLEQIIKNHDISREEGLPICQDTGMAVVFADIGQDVRFVGGDFTVAVNHGVEQAY
                     SEGFFRKSLVRDPLFDRTNTGTNTPVVIHVSIVPGDRVGLLVTPKGFGSENMSAMKVI
                     TPAEGADKVVEFVRDTVVAAGPNPCPPTIVGVGVGGTIEKAALMAKRSTLRPVGKRNG
                     DPRYAALEETILDAVNSTGIGPGGFGGRVTCLDVHVDFTAGHIAGTPVVVNICCHAAR
                     HAEVTI"
     gene            2261..2842
                     /locus_tag="P5541_04695"
     CDS             2261..2842
                     /locus_tag="P5541_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRV71310.1"
                     /translation="MMVQERKIISVPLSRETALSLYAGDRVLIDGNLYTGRDAAHRKM
                     FDSLGKGDPLPFDIEGQIIYYVGPSPAPPGKIIGSAGPTTSGRMDPFTPALLDLGLRG
                     MIGKGYRSEKVIRSMVRNGAVYFGAVGGAAALIARSIIQAEVVAWPELGPEAVFRLKV
                     RSFPAIVVIDAFGNDVYRTGPPVYRAGRDGGES"
     gene            2839..3564
                     /gene="pxpB"
                     /locus_tag="P5541_04700"
     CDS             2839..3564
                     /gene="pxpB"
                     /locus_tag="P5541_04700"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRV71311.1"
                     /translation="MIKETSGQTGFPRFFWAGDSCVVAEFGDCIDMDLNARVRLGARR
                     LEEMAPSWLVETVPTYRSLAVYIDPWSTSREEAMKMVQELCEVTGSFLLPEPERLDIP
                     VCYGGEFGPDMERVCSHAGLVLEEVVELHSAPEYHVYMIGFSPGFPYLGGMDPRIETP
                     RLGNPRTAIPAGSVGIAGMQTGVYPTETPGGWNLIGRTPLRLFDPGKNDPFLLSAGMK
                     IRFVPVDRSVFQEFSEDLMEPER"
     gene            3561..4610
                     /locus_tag="P5541_04705"
     CDS             3561..4610
                     /locus_tag="P5541_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRV71312.1"
                     /translation="MITIESPGLLTTVQDRGRWGYQAFGMPVSGVMDIYSLMAGNAVV
                     CNEPGAAALEITLLGPSVRFGSSRMFCVTGGDLAPRLNGRDIPSWEGVVAREGDLLSF
                     HGAIQGARAWLCISGGIDTPPVMGSRSTCLRAGIGGHQGRKLVAGDVIPLGRPDRLWY
                     RGEGFSIPVHFRNKAEDCPAIRVIQGPQEEMISAAGLEAFYGSVFRVSPESDRMGYRL
                     EGKRVEHAGGADIISDAIPLGAVQVPGHGSPIIMLADRQTTGGYPKIAVTIGADIPIL
                     AQISPGDPLSFRKADMAEAETALRSMSEHLYHVRDMAADWRSRYGKRMVPKREGNMIL
                     RIEGKGYDVSWEILE"
     gene            4647..5417
                     /locus_tag="P5541_04710"
     CDS             4647..5417
                     /locus_tag="P5541_04710"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRV71313.1"
                     /translation="MKSRVDLNCDLGEGFGPWRMGSDEGIMDHVTSVNVACGFHAGDP
                     VVMKDTVERAVARGIAIGAHPGYPDIQGFGRREMSCTPDEIYALCLYQIGALMAFARS
                     CGVRLAHVKPHGALYNRGARDKEAAFAIARASRDADRNMVLVGLPGSAFEKAAIEAGI
                     PFASEAFADRAYMEDGSLVPRSWPGSVIHDPEEAARRVLEMVKEGRIISVEGSMLHIR
                     PDTVCVHGDTPEALRMARAIREKLEAEEIVIAPVGSGS"
     gene            5621..7321
                     /locus_tag="P5541_04715"
     CDS             5621..7321
                     /locus_tag="P5541_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRV71314.1"
                     /translation="MKGAFFVSKKEVCKDVLEGKKDYDGKVAKNLSRQPEMKESFKTI
                     GGIPLERLYTEIDLQDTDYCRDIGFPGSYPYTRGVQPTMYRGRFWTMRQYAGFATAAE
                     SNARYRYLLDHGQTGLSVAFDLPTQIGYDSDHPMARGEVGKVGVAIDSLADMEVLFDK
                     IPLGKVSTSMTINAPASVLLAMYIAVGEKQGVKASDLSGTIQNDILKEYIARGTYIFP
                     PKPSMRLITDIFGFCSDNVPKWNTISISGYHIREAGSTAAQEVAFTIADGLAYVKAAM
                     DAGLDPNVFGQRLSFFFNAHNDFLEEVAKFRAARRLWARLMKERMGVTEPKAMMLRFH
                     TQTAGSTLTAQQPENNIVRVAIQALASVMGGTQSLHTNSFDEALALPTEKSVGIALKT
                     QQIIAYESGVTNTVDPMAGCYVVESLTNEIERQAMEYIEKIGEMGGMLVAIEKGYPQQ
                     QIQDAAYQFQREVESDERIVVGVNKFQIDENGGGRELLRVDPSVGANQEKALGKMKQG
                     RDNVLVSNRLDAVRAAARDESVNLMPVIIDAVRAYATEGEICGVLREEFGEYRENIVL
                     "
     gene            7350..7763
                     /locus_tag="P5541_04720"
     CDS             7350..7763
                     /locus_tag="P5541_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRV71315.1"
                     /translation="MSDRRIRVIVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PESIVETAIQEDADAIGISILSGAHEHYFSEIISLLKSKNAEDIIVFGGGVIPEGDIP
                     GLMEMGAGAIFGPGTPTKTTIEWLVEAVGKRGVKN"
     gene            7895..8299
                     /gene="mce"
                     /locus_tag="P5541_04725"
     CDS             7895..8299
                     /gene="mce"
                     /locus_tag="P5541_04725"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRV71316.1"
                     /translation="MKPTAIDHIGIAVVSIEESLKFWEGSLGIKCAGIEEVAEQKVKT
                     AFLPLGDTEVELLEATDPDSPVAKFIEKKGEGIHHLAIRVENLEEALEKMKAMGLKMI
                     DEKPRYGAGGAKIAFVHPKTAGGVLLELSERD"
     gene            8351..9910
                     /locus_tag="P5541_04730"
     CDS             8351..9910
                     /locus_tag="P5541_04730"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRV71317.1"
                     /translation="MSERTIGELCENLERFREEAEAGGGEKAIAKQHEKGKLTARERI
                     DKLLDEGSFVELDAFVEHRCVNFGMEKKAYPGDGVVTGYGTVDGRLVYVYSQDFTVMG
                     GSLGEMHAKKICKVMDLAMQNGAPVLGLNDSGGARIQEAVDSLSGYGNIFYRNTIASG
                     VVPQLSIIVGPTAGGAVYSPALTDFIFMVDKIGILHITGPAVIKAVTGEDVTSEQIGG
                     AMAHNQISGNAHFFASTEEECFGQIRKMLSYLPSNNMEEPPFVDAGDDPYRTDMSLRD
                     LVPTNPNKGYKVQDVLTRIADGGEFFEVQPMWAKNMVTGFMRVGGYVTGIIANQAAIM
                     AGCLDIDASDKASRFIRYCDAFNIPIVTFVDVPGYLPGKAQELGGIIRHGAKLLYAYS
                     ESTVPMITVVLRKAYGGAYLGMCSKDLGADVVLAWPQAEIAVMGAEGAGNIIFKKEIE
                     SAPDPQAMRTQKIEEYRDAFATPYQAAKRGFVDRVILPEDTRGEIYKALVQCESKRQL
                     RPRRKHGVMPH"
     gene            9930..10319
                     /locus_tag="P5541_04735"
     CDS             9930..10319
                     /locus_tag="P5541_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRV71318.1"
                     /translation="MELSHIADKFSHGLGGAFALAMIAFSIVFLVLMALTFIIMGTRY
                     LADLSEGKKQTPKTPQAQQAAATSGAAPQASAGTDRNVIAAVIAAAIAASGGGRAISV
                     RPLTVSNRGKSSAWKTAGRMDLLEGFE"
     gene            10494..10877
                     /locus_tag="P5541_04740"
     CDS             10494..10877
                     /locus_tag="P5541_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRV71319.1"
                     /translation="MSKKYKITVNGTAYEVEVEDIGGSSAPAPAAAPAVAPSPAAAPA
                     PAAAPASAAAPAGGTTVTAPMPGKVLKVAVSVGAAVNAGDLLLVLEAMKMENEIQAPS
                     AGTVKEIKVSDGSPVNTGDVLVVLG"
     gene            10913..12037
                     /locus_tag="P5541_04745"
     CDS             10913..12037
                     /locus_tag="P5541_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRV71320.1"
                     /translation="MGLYFRALAEIAEQSGFAGLTTGNLIMLVVALVLLYLAIAKGFE
                     PLLLVPIATGCLLVNLPLSGIMDEGGFLRYLFFGAEHEIYPVIIFMGIGALTDFSPLL
                     ANPMTFLLGAAAQLGVFVALFGAMVLGFSVPEAASIAIIGGADGPTSIYLTMKLAPQI
                     LGAVAVAAYSYMSLVPLIQPPVIKMFTTKKDRAIRMDALRPVSKRERILFPIVCTVSA
                     GLILPASVPLIGLLMFGNLLRECGVTERLNQAAQNEILNTVTILLGLSVGATMGAETF
                     LTVATIKIILLGLVAFIFSTAGGTLFGQLMKVLSGGKINPMIGAAGVSAVPMAARVVQ
                     RISAQENPSNFLLMHAMGPNVAGVIGTAVAAGAMLTLLTN"
     gene            12225..13514
                     /locus_tag="P5541_04750"
     CDS             12225..13514
                     /locus_tag="P5541_04750"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRV71321.1"
                     /translation="MNLSMFLSNEVKPALGCTEPGAVALAASWAARYSEGDIKGIKLL
                     LSPNIFKNGLHAGIPGTGGETGNLLAAALGAMKGDPDKGLTALEGITGDDVSRALDFV
                     KTGGVTQQVLQDVPDVYVQAEVVSDSGTVSVIISGRHDRIVEIRKNGDVIKRLLEADT
                     EGGGRPPYIDDLLEKDMEWFWSSASSIDAGMEKFLIEGSRVNLKVAETGLQKPWGLGT
                     GYHLAALGGMDDLLRQVKAWAAAAADVRMAGGAWPVMSSAGSGNHGITAIIPPSLAAR
                     VWGKSERELAEALMLSHLVTGFIKAHTGRLTPVCGCAVAAGAGAAAAIVRLGGGTANQ
                     GEIAVALLLSSLQGMVCDGAKGSCALKVSTAAGESFISAQLALGGIGDPGEQGIISSN
                     FVEAARNVGNFSRNGLSGADSAIIRILQEMEASGKNS"
     gene            complement(13530..14327)
                     /locus_tag="P5541_04755"
     CDS             complement(13530..14327)
                     /locus_tag="P5541_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV71322.1"
                     /translation="MFRKILFPTDFSELSHKGLLWTAANVADGSSEIVLVHVVEPMTG
                     LDTSRMIQEAEKAMEKTVSELGTTGIKCSSLVLSGDPLETLSETAHSRGCSVSVLFAE
                     SNDVVVNFIRYMAIPHLVVKTSETKVPFQNPFNHVAVCTDLSPERTDRVLEELRNLLD
                     GRNVPMTIIHSVSLDDPSTSSDMFQIASSALEEVRESVASWNSDVKAEVLAGEPEIEI
                     LRKTREMTPGMLVVGLSTHGQLWELIIGSTGEAIIEKSPCPVLVIPT"
     gene            14426..15310
                     /locus_tag="P5541_04760"
     CDS             14426..15310
                     /locus_tag="P5541_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV71323.1"
                     /translation="MSPSPNFTWTVLSGFARIVSILPHRVSVSIGAWTGLVVWFFSRS
                     RVDRAEARCVKALQVGVTRARGIVRESYVNLGRSLAEFLRLPVLGKKVLDYVDIHGED
                     NLRIALERGKGVIFLTAHFGNWEYNASTMAVRGFPMNAIGAEQRDPRITELVVSLRSA
                     CGVKTMSKGFDLKSAVRCLREGEILGILLDQDFADNGIVIPFLGIPASTPFGPVKMAG
                     KIGSSVVPTFIVRRKDGINHDLYILPPLDFAGGKPFGEDVEASLKLCNDTLGEWIRKH
                     PDQWMWLYPRWASTTGDR"
     gene            15311..15784
                     /locus_tag="P5541_04765"
     CDS             15311..15784
                     /locus_tag="P5541_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRV71324.1"
                     /translation="MIAAVDPGREKFGWAICDEAGQLFLSGVSPVGEVCRWGGALAAA
                     DYEYLESIALEKRGPFQIENRQFLLLAGSGTGSGHVIEMLRQTGLEVFAVPEENSTLR
                     GRALFWKIHPPKGLARLLPRSLAVPWRCADDMAAWCLVLEHLERQRSKNIDRSGG"
     gene            15790..16125
                     /locus_tag="P5541_04770"
     CDS             15790..16125
                     /locus_tag="P5541_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71325.1"
                     /translation="MSYSSRLKVYIEQPGFSVGCVDLEYSEEEMKSFPMASGRLQVGK
                     ILVKLREKYGDDLRVDLIDPRSLTAMLDILKYRIKSTEPTWILDGKLIFRGVPEWEEL
                     QAIIDAFPG"
     gene            16149..17576
                     /locus_tag="P5541_04775"
     CDS             16149..17576
                     /locus_tag="P5541_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRV71326.1"
                     /translation="MKKWFSDVAIEELVLGYAFVKWIILAVFAGLIVGSAASLFLLLL
                     DKGVAGGLSLGPARLLLLPPGLLASVFLVRKMAPDAAGHGTEKVIEAIHQRSGRISLP
                     TVPVKLVATVITIASGGSAGKEGPCAQIGAGLTSAMASLMGLNDTDREKLVVCGISAG
                     FSAIFGTPVAGAIFGLEVLFIGKMYYDVMLPAFISGMISFQTASLLGVVYAEQKLSSL
                     TTLGGTTLLWATLAGVFFGLVALAHIEIVSASDKWISRIPAGPYIKAAGGGVVLLVLA
                     MLFGTRYLGLGTDFIEEAISGSNVPGGAFLLKSLFTGITLSAGGTGGILTPTFFVGAG
                     SGSFFASLFGLDGALFSSIGLVAVLAGAANTPISATIMAIELFGSEVASYAAIACIVS
                     FAISGHRSIYPSQILARPKSSVFGVSKDGLPVEFHENRINLSDTRIPSMLLEAKAYWE
                     RRVFFPRDMESPAEVKQPKDVQEGE"
     gene            complement(17561..18265)
                     /locus_tag="P5541_04780"
     CDS             complement(17561..18265)
                     /locus_tag="P5541_04780"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRV71327.1"
                     /translation="MSKKPHKVLGILGGMGPAATAEFLRLLAEMAGAEKDQDHPRIVL
                     FSNPQIPDRTAAITGGGEDPAPLLLEGLKTLQSWGAQLLAVPCNTAHVFINRFRDRLS
                     VPLVHIVEETISRAMRTSPGGAWLTATSGTSSSRIYETEAKKRDYPLLIPGPETQNML
                     EEVIALVKANRLSESAARLESCLRILWSEKDIPVIGACTELPLAYRSSAMDQEKIISS
                     LDSLALACLERIYSPS"
     gene            complement(18246..19469)
                     /locus_tag="P5541_04785"
     CDS             complement(18246..19469)
                     /locus_tag="P5541_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRV71328.1"
                     /translation="MLAEKKKMPLIWKIAIGFVAGILAGIVFGDSISFIAPVGTIFVT
                     LLQMLVVPLVFSSLVVGTASLGDPRALGRVGIKTIGLYLATTAVAIAIGLLLGNIIQP
                     GVGMGLGADLSGFKVPTPPSTSDVIIGLFPKNPISSMASGHMLQIIVFALFLGISAVF
                     AGEKGKPLIGLMDSVAETMYKMTGLVMHVAPYGVFALIAATVAKYGVSVLAPFGKVIF
                     GVYLGCILHALLVYSGLLVSIVHMSPWKFFDGVKEATLTAFVTRSSSATLPITMRVTQ
                     ENIGVSEKISSFVLPLGATINMDGTALYQGVCALFVAQAFGIPLSIGAQLGILLTATL
                     ASIGTAGVPGAGLIMLTLVLTQAGLPVEGVALIAGIDAVLDMARTAINVTGDATVATV
                     VAKTEGELADVKKAA"
     gene            19727..21808
                     /locus_tag="P5541_04790"
     CDS             19727..21808
                     /locus_tag="P5541_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRV71329.1"
                     /translation="MIMRGLIVMLLIALAASGSYAGGVVLVLSGGGTRGLAHVGVLRV
                     LEDNGISVEGIVGTSIGSIVGGLAACGYSSGELEDMLAGLDMTSLLYDRQREAQSPPG
                     EEGTDSSLSLFRLEFSERGHITGPLGGLSGKRLLERLQEWTVRCPVLDFADLPVPFAA
                     VATDLVTGEPVVLRRGNLASAMRASMAIPGLFSPWNVDGRLLVDGGLVSNAPVLVARE
                     LFPGYPVVVVDVSGKGKSRDDIRTVVDVMDQMISIMTMRNVQEELKHADLVITPDVGG
                     LPMLDVAGHEEIIKSGELAARDKLDQIISVASKTTPSSPLKASEKIIVAEVRTTGLGE
                     RFSQAVIDRYDLWVGKEADPESIIQACVDLRQRDDVKAADFSLEYLPDGRAAVVLNVE
                     KEPAWEVVAGGYASNLNPYAAVYLDTIRRDLFSEGDSLRTRFGISERWHFSARYLSPV
                     EEGYGRWEANIKGGKRIYSPPGSGTLEWEQYSASLWRHYGDGPFRASLGFAVEETRSG
                     GQTNTYTGPVLSLAFEGLDDPIDPTEGFSASLSLWWPDTDELLGRMRFFGVTSSAKDW
                     RFYIRGGAILGDGSRAWHSAYLGAREELYSRGASPLRSSNAAWAGVGIRKVFLKSWWG
                     TINLDLFATAGRTYDSSWSGVDKIWETGIAVSLPGKIFDGKILVIYDDRSDWSFGFVL
                     GRPLWENDPLP"
     gene            21909..23348
                     /gene="proS"
                     /locus_tag="P5541_04795"
     CDS             21909..23348
                     /gene="proS"
                     /locus_tag="P5541_04795"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRV71330.1"
                     /translation="MARNIAPREKDYSQWYLDVIKAAELADYAPVRGCMVIRPDGYAL
                     WESIQHFFDEAFKDSGHVNAYFPLLIPISFLEKEADHVEGFAPECAVVTHAGGEELEE
                     ALIVRPTSETVIGHMYSKWVQSWRDLPILINQWCNVMRWEKRSRLFLRTSEFLWQEGH
                     TAHASREEAVEETLRMLEIYRDIMENVLALPVLAGEKSEGERFPGADNTYTCEAMMTD
                     RKALQAGTSHFLGQNFAKAFEINFQNREGELESAWTTSWGVSTRLIGAIVMTHSDDDG
                     LVIPPRVAPVKAVILPISPDQEMVEGKLLPRAAEIAEKLNAVLGKRSVKIDKNFHMRP
                     GDRFFASLQKGIPLRLELGEKEFGKEVLHAVRRDTGERFDIPWDEVATRVPEVLEDIQ
                     KNLYEKALAFREKNTIHVTGYEEFSKIFEGSGGFVEAFFAGGREEEKKIKEETGATVR
                     CFPLGREETGTCFYTGKKGARLAVFAKSY"
BASE COUNT         5509 a         5704 c         6906 g         5416 t
ORIGIN      
        1 ccgaaaggca actcgcctga aagcgttcgt ttctccgcct gcttcggggc gaccgcacag
       61 tgccggggtg aaccctccgg caggtgtgct tttcctgccc ctacgcgacg gctgcgaaac
      121 tcaccggctc ctgagccttc ggtaaagcct tcactccggt cccggtcccg accttgccgt
      181 ttccgttcac cgttcaccgt tcacgcagtt ggctttaagg cccttgccct tcctgtctcc
      241 tggtttttta aaaatctaaa actcaagtat tgttcctatc ccgatgcggc aggccttgtc
      301 gtagattacc cttgccgtta cgaggtcgtg gcttgcgagg cctatcagca ctgctgctct
      361 ccggcccccg ttttggggtg cttccatttt tcccgcgcaa atttcaccca tatcggccag
      421 tacttcggac gggtactcac tgaaatacgc gccgccctcc ttcatccaga ggtactgggc
      481 gcggtcatcg gtagtgaaga aagaggcctc tgtcatgaca ctggcctcga aagcggaatc
      541 ataatccaca gaaatggcca gaacatcctt tttcaaccac tccgcaggaa taaaacgctc
      601 tggttcacgt cttatcgggg tacaggtaac gactatatcc gatcccctgg tcgcgctttc
      661 agggtcagag aagatctcta tttccagttc cggcgctttc ctctgcattt cctcaagaaa
      721 cttccgggag tgatcgggaa taatgtcgaa aaccctgatg ttacgaagcc ccttcatggt
      781 ctcccgaaga gctatgagct gtgttctggc ctggacaccc agacctatga tagagagggt
      841 ggaagcgccc ttcggtgcca gtttgtttgc cgttattgcg gttgccgcac cggtcctgta
      901 ggcagtgatc cagcccgcat ccatcaccgc cagaggaaca cccgtctccg cgtcgttgag
      961 gattatcagt cccattatca gaggcagtcc cttttccggg ttcgacggat atctggagac
     1021 ccatttgatg ccagccgtat cggcgatgcc cgaaatgaaa gaaggcatgg catgaatgta
     1081 gcaatcttcc cgtgggtgga cttcaggttt tctcggaagc tggaccatgc cttcaccttt
     1141 aagcgtgaag ccttcttcga cagcctcgat gacctctttt actggtatcc tcattgattc
     1201 gatctggctt cggttcaaat aaagaatgtc caattgtttt gcctcccgga taaagtatgg
     1261 aaattagaat tgttcgacct tattatacgc ctgaggggcc ttgaaaaaaa cccctgaact
     1321 gtatgaaatg aatttaccaa gaggttatac ttgcctgaaa gaagacagac catatctttg
     1381 gaaagaggta tccgggttga gagaagtcca tgtatcttcc attgaggaag cggtaaaaaa
     1441 cctgttcatc aaggccaata catcaatagg acaggatgtc tataacgccc tggtccacgc
     1501 tcttggaaaa caggaggatt cgccgacagg cagggatgtt ctcgagcaga taataaaaaa
     1561 tcacgatatt tcccgtgagg aaggtcttcc catttgccag gatacgggca tggcagtggt
     1621 ctttgccgat attggacaag atgtccggtt tgtgggagga gatttcactg ttgctgtcaa
     1681 ccatggagtg gagcaggcct attcagaagg atttttccgg aaatccctgg tcagggatcc
     1741 ccttttcgat cgaaccaata cagggaccaa taccccggtg gttattcacg tatccattgt
     1801 tcccggggac agggtgggac ttctggttac acccaagggc tttggaagcg aaaacatgag
     1861 cgccatgaag gtaattacgc ctgcggaagg ggcggataaa gtggtcgaat ttgtcaggga
     1921 taccgtggtc gcggccggcc ccaatccctg tccacccacc atcgtgggtg ttggcgtcgg
     1981 cggcacaata gaaaaggcag ctctcatggc gaagagatcc acccttcgac cggttggaaa
     2041 aaggaacggg gatcctcggt atgctgccct ggaagagacc atactggacg ccgttaacag
     2101 tacaggtatc ggacctggag gtttcggagg tcgggtcaca tgcctggatg tccatgttga
     2161 ttttaccgcc ggacatatcg ccggaactcc cgtggtcgtt aatatctgtt gccatgcggc
     2221 cagacacgcg gaagtgacca tatagaatgg ggaccaagtg atgatggttc aggaaagaaa
     2281 aataatctcc gtacctcttt caagggagac agccctgtcg ctttatgcag gtgacagagt
     2341 cctgatcgat ggcaaccttt atacgggacg ggatgctgcc cacaggaaaa tgttcgactc
     2401 tctgggtaag ggggatcctc tccctttcga cattgaaggc cagataattt attatgtcgg
     2461 gccttcaccc gccccgccag ggaagatcat aggttccgca ggacccacga ccagtggaag
     2521 gatggatccc tttaccccgg cccttctgga cctgggtttg agagggatga taggcaaggg
     2581 gtacaggtcc gaaaaagtta tccggtccat ggtcagaaac ggggcagtat atttcggggc
     2641 tgtagggggg gctgccgccc ttattgcaag gagcataatc caggccgagg tggtcgcgtg
     2701 gcccgaactg gggccggagg ccgtgttccg cctgaaagta aggtccttcc cggccatagt
     2761 ggtgatagat gctttcggaa atgatgtcta tcgtacgggg cctcccgttt acagggcggg
     2821 aagggatgga ggagaaagtt gataaaagaa accagcggtc aaaccgggtt tccgcgtttt
     2881 ttctgggcgg gagactcctg cgtggtggcc gaattcgggg actgtatcga tatggatctc
     2941 aacgccagag tgagacttgg agcgaggaga ctggaagaaa tggccccatc ctggctcgtt
     3001 gagacggttc cgacctacag gtctctggcc gtctacattg acccctggtc gacctccagg
     3061 gaagaggcta tgaaaatggt ccaggaactc tgcgaggtga cggggagttt tctcttgccc
     3121 gagccggaga ggttggatat ccccgtttgc tacggggggg agttcggacc cgatatggag
     3181 agggtgtgct ctcatgcggg gctggttctc gaggaagtgg tggagctaca ttcagcacct
     3241 gaatatcacg tctacatgat cggtttctcg cccgggtttc cctatctggg aggaatggat
     3301 ccaaggatcg aaacacccag gctcggaaat cctaggactg cgataccggc cggaagcgtc
     3361 ggtattgccg gaatgcagac aggtgtatac cctacggaaa caccgggagg ttggaacctt
     3421 atcggcagga ccccgttaag attgttcgat ccgggaaaaa atgacccctt tctattgagt
     3481 gccggaatga agataaggtt tgtgcccgta gaccggtccg tttttcagga attttctgaa
     3541 gaccttatgg agccggaacg atgataacca tagaatcgcc ggggcttttg acgactgtgc
     3601 aggacagggg gagatggggc taccaggctt tcgggatgcc ggtatccgga gtgatggata
     3661 tctattccct catggcgggt aacgcagtgg tttgtaacga acccggtgct gccgccctgg
     3721 agataactct cctgggaccc agtgtacgtt tcggttcttc ccgaatgttc tgtgtgaccg
     3781 ggggggatct tgctccaagg ctgaacggca gggatatccc ctcctgggag ggcgtcgtgg
     3841 caagagaagg ggacttgctg tccttccatg gtgctatcca gggagcaagg gcctggttgt
     3901 gcatcagcgg aggcattgat acgccgccgg taatgggaag caggtccacc tgtcttcgag
     3961 ccgggatagg aggtcaccag ggaagaaaac tggtcgccgg ggatgttatt ccccttggca
     4021 gacccgacag gctctggtac aggggagagg gattctccat accagtccat ttcaggaaca
     4081 aggcggaaga ttgtccggca ataagggtta tccagggccc ccaggaggaa atgatctccg
     4141 cggcagggtt ggaagcattt tacgggtctg tttttcgtgt ttcgccggag tcagacagaa
     4201 tggggtatcg ccttgaaggg aaaagggtgg agcatgccgg aggtgctgat atcatttccg
     4261 atgccatacc tctgggggct gtgcaggtgc cgggtcacgg gtcgccaatt ataatgctgg
     4321 cggacagaca gactaccggt ggctatccca agatagctgt aacaataggg gcggacattc
     4381 ccattcttgc ccagatttcg cccggggatc ccctttcttt caggaaagcg gatatggctg
     4441 aggcagaaac agcattgcga tcaatgtcgg aacacctcta tcatgtgcgg gatatggctg
     4501 cggactggag atcgcgctac ggtaaaagga tggttccaaa gagagaagga aatatgatcc
     4561 tgcggataga gggtaagggc tatgatgttt catgggaaat cctggaatga agtttttata
     4621 aatgaataaa ttcggaggag tcgctcatga agtcaagagt tgatctcaac tgtgacctcg
     4681 gagaaggttt tggcccatgg cgcatgggca gcgacgaagg gatcatggat cacgtgactt
     4741 ccgtgaacgt ggcatgcggt ttccacgcgg gagaccctgt cgtgatgaag gatactgttg
     4801 aaagggctgt ggcgagggga atagcgatcg gcgcgcatcc aggctaccct gacatccagg
     4861 gattcgggag aagagagatg agctgcaccc ccgacgaaat atatgcctta tgtctctacc
     4921 agatcggggc tttgatggct tttgcgagat cttgcggagt ccgcctggcg catgtgaaac
     4981 cccacggagc tctctataat cgtggagcga gggacaaaga ggccgcattt gctattgccc
     5041 gggcgtccag ggatgccgac aggaatatgg tgcttgtagg acttccgggt tcggcttttg
     5101 aaaaagccgc gatagaagcg ggcatacctt ttgcctcgga ggcttttgcc gacagagctt
     5161 acatggaaga tggatctttg gtgccaaggt catggcctgg ttcggtgatc cacgatccgg
     5221 aagaggctgc gagaagagtt ctcgaaatgg tcaaggaagg aaggattatt tcggttgaag
     5281 gaagcatgct tcacataaga cctgatactg tctgcgttca cggggataca ccggaggcct
     5341 tgcgcatggc acgggcgata agggaaaagc tggaagcaga agagattgtc atcgcaccag
     5401 ttggttctgg ctcgtgaaat cagaagggat cagttgtttt aattcgctga aatgtatgta
     5461 attgtctcgc aattcaatga aatggttgca tcaaagaccc tgttggggaa taatagtcgc
     5521 gtttgtcgtt ttattcatgt tttggttgtt atttgaagca cagttcagta tattctgcac
     5581 tttatgaatg ttgtttttaa cagcaaaggt cgatccggaa atgaaagggg cgttttttgt
     5641 gagcaaaaag gaagtttgca aggatgtgct tgagggcaaa aaagattacg acggaaaagt
     5701 tgcgaaaaac ctgtccagac agcccgaaat gaaagaatct ttcaaaacga tcggtggcat
     5761 ccctctcgag aggctctata ccgagatcga cctgcaggac accgattact gcagggatat
     5821 cgggttcccc gggtcctacc cctatacgag aggcgtccag ccaacgatgt atagggggcg
     5881 tttctggaca atgcgccagt acgcaggatt tgctaccgca gcggaatcca atgccagata
     5941 tagatacctg ctggatcatg ggcagaccgg tctttcggta gccttcgatc ttcccaccca
     6001 gataggctac gattcggatc accccatggc caggggggag gtcggcaagg tgggtgtcgc
     6061 cattgacagc cttgctgata tggaggtgct tttcgacaag ataccccttg gcaaggtttc
     6121 cacatcaatg accataaacg ccccggcttc ggtgctgcta gccatgtaca tagcggtggg
     6181 agaaaagcag ggagttaagg cttcagatct ttcaggcact atccagaacg acatcctcaa
     6241 agaatacatt gccagaggaa catacatctt cccaccaaaa ccttccatgc ggctcattac
     6301 tgatatcttc ggattctgca gtgacaatgt tccaaaatgg aacaccatat cgatctccgg
     6361 ttaccatatc cgcgaggctg gttcaacggc ggcccaggag gttgccttta ccatcgccga
     6421 cggtctcgct tatgtaaaag ccgcgatgga tgccggtctt gatcccaatg tcttcggcca
     6481 gagactttcc ttcttcttca atgcccacaa tgatttcctt gaagaggttg ccaagtttcg
     6541 tgccgcaagg cgtctttggg cccggctcat gaaggagcgc atgggggtta cggaacccaa
     6601 ggccatgatg ctgcgtttcc atacccagac tgcgggaagc actcttactg cccagcagcc
     6661 ggagaacaac atagtcagag tggctatcca ggcccttgcc tctgtgatgg gggggaccca
     6721 gtcgctccac accaacagct ttgacgaagc cctcgcgctt cctactgaga aaagcgtagg
     6781 gatagccctg aagacccagc agataattgc ctacgaatcc ggtgttacca acaccgtaga
     6841 cccgatggcc ggttgctatg ttgtcgagag tctgaccaac gagatcgaaa ggcaggccat
     6901 ggagtacatc gagaagatcg gtgagatggg tggaatgctt gtggccatcg agaaaggcta
     6961 tccgcagcag cagatacagg atgcggctta ccagttccag agggaagtcg aaagcgatga
     7021 gcggatcgtg gtcggtgtca acaagttcca gatcgatgaa aacggcggag gaagggagct
     7081 tctgagggtc gatccctcgg tgggagccaa ccaggagaag gcccttggca aaatgaaaca
     7141 ggggcgggac aacgtccttg tctcgaatcg tcttgatgcc gttcgtgccg ccgcaaggga
     7201 cgaaagtgtc aatctgatgc ctgtcatcat agatgcggtc agggcctacg ccaccgaagg
     7261 agagatctgc ggggtccttc gcgaggagtt tggcgagtac agggagaata tcgtccttta
     7321 gggacgttct ggatcaggag ggactcatga tgagcgatcg caggatacgt gtaatagttg
     7381 caaagccggg gcttgacgga catgacaggg gtgccaaggt cgtggcgagg gccctgcggg
     7441 acgccggtat ggaagttatc tacaccggcc tcaggcagac ccccgaaagc attgttgaaa
     7501 cggcgataca ggaggatgct gacgccatag ggataagcat cctcagcgga gcccacgagc
     7561 actatttttc agaaataatt tcgcttctca agagcaaaaa cgccgaagac ataatagttt
     7621 ttggtggagg agttatcccc gagggggata tacccgggct tatggagatg ggggctggag
     7681 caatattcgg tcccggaaca ccgacgaaga ccactattga atggcttgtt gaggctgtgg
     7741 ggaaaagggg agtcaagaac tgacgtctct ttcgtttttg caacacctga catttagcgg
     7801 ctcgacaagc aggtatatgt agccatatga atatcccgct tcgggattaa aaatatcggg
     7861 gatcccttgg aaaagggccg gatggaggca gataatgaaa cctacggcaa tagatcatat
     7921 cgggatagct gtcgtgagca tcgaggagtc tctcaagttc tgggagggat ctctcgggat
     7981 caagtgtgcg gggatcgagg aggtcgccga gcagaaggtt aaaactgctt ttttgccatt
     8041 gggtgatacc gaggtagagc ttcttgaggc gactgatccg gatagccccg tggcgaagtt
     8101 catcgaaaag aagggcgagg gtattcacca cctggccata agagtcgaga acctcgaaga
     8161 ggctctcgaa aaaatgaagg ccatgggact gaaaatgatc gatgaaaaac cgaggtatgg
     8221 tgcgggcgga gcaaagatag ccttcgtgca ccccaaaaca gcgggtggtg ttctgctgga
     8281 gctcagcgag cgggattgaa ctttcgacgg cggcgggccg ggaaattttt gttcaaaggg
     8341 aggaaaagag atgtctgaga ggacgattgg ggagctttgc gagaacctgg agcgtttccg
     8401 ggaagaagct gaggccggag gtggcgaaaa ggccattgca aagcagcatg aaaagggcaa
     8461 actgacggcc agggagcgaa ttgacaaact tcttgacgag ggaagtttcg tcgaactgga
     8521 cgctttcgtg gagcatcgct gcgtcaactt cgggatggaa aagaaagcct atcccgggga
     8581 cggggtggtc accggttacg gtacggtgga tggaagactg gtctatgtct acagccagga
     8641 ctttacggta atgggcggct ccctgggaga gatgcacgca aaaaagatct gcaaggtgat
     8701 ggacctggcc atgcagaacg gcgctcccgt tcttgggctc aacgacagcg gtggggccag
     8761 aatacaggag gctgtcgaca gcctcagcgg atatggaaac atcttctacc gcaataccat
     8821 tgccagtggt gtggtacccc agctttcgat catagtgggt cccacggcgg ggggtgcagt
     8881 ttacagcccg gcgctgacag acttcatctt tatggtggac aagataggta tcctccacat
     8941 aaccggcccc gcagtgatca aggctgtgac aggtgaagac gtgacaagcg agcagatcgg
     9001 tggagccatg gcccataacc agatctccgg caacgcccat ttctttgctt ccaccgagga
     9061 agaatgcttt ggacagatcc ggaagatgct ttcctatctt ccctccaaca acatggagga
     9121 accacccttt gttgatgcgg gtgacgaccc ctacaggacc gacatgagcc tgcgcgatct
     9181 tgtgcctacc aatcccaaca agggctacaa ggtacaggac gtacttacgc gtatagctga
     9241 tggcggcgaa ttcttcgaag tccagcccat gtgggcaaag aatatggtta ccggattcat
     9301 gagggtcggc ggatacgtta ccggaataat cgccaaccag gcagccatta tggccggatg
     9361 tctcgatata gatgcttccg acaaggccag caggttcatt cgttactgcg atgccttcaa
     9421 cattcccatc gtaaccttcg tcgatgttcc cggctatctc ccgggcaagg cacaggaact
     9481 tggagggatc ataagacacg gcgccaaatt gctctatgcc tacagcgaat ctacggttcc
     9541 gatgataacg gtggtcctcc gcaaggctta cggcggtgcc tatctcggga tgtgcagcaa
     9601 ggatcttgga gccgatgttg tccttgcgtg gccccaggca gagatagcgg tcatgggtgc
     9661 cgagggggca ggcaacatca tcttcaagaa ggagatcgag agcgccccgg atccgcaggc
     9721 tatgaggacc cagaagatcg aggagtacag ggatgctttc gctaccccgt accaggcggc
     9781 aaagaggggt tttgtcgaca gggttatcct tcccgaggac acgcgaggag agatatacaa
     9841 ggccctggtt cagtgcgaga gcaagcgcca gcttcgtccg cgccgcaagc acggcgtaat
     9901 gccccactag ccgaaggagg tagttgttca tggaactttc tcacatagcg gacaaattct
     9961 cccacggtct tggaggagcc tttgccctcg cgatgatagc cttttcgata gttttcctgg
    10021 tgctcatggc gctgaccttt atcatcatgg ggacgcgcta tctggcggat ttgtcggagg
    10081 gtaaaaagca gaccccgaaa acaccgcagg cacaacaggc ggcagctact tccggtgctg
    10141 ctccccaggc ttcagcgggg acagacagaa acgttattgc cgcggtcata gcggctgcta
    10201 ttgctgcctc tggtggagga agggccatat cagttcgtcc acttactgta agcaacaggg
    10261 gaaaaagttc tgcgtggaag accgctggaa gaatggacct cctcgagggg tttgagtaag
    10321 aagggtaaag ggtaaacggt caacggtaaa cggttattta gatctatgat caaggttgca
    10381 ccgttcactg tttaccgttc acggtttacg catttcatat tggaaaggcg aatgaatttc
    10441 aaaaaatttc gaatcaaaat ttgaccgaat aaataaggag gaaaaacaaa gctatgtcca
    10501 aaaaatacaa gatcaccgta aacggaacag catacgaggt tgaagtagag gatatcggcg
    10561 gatcatcggc ccctgctcct gcagccgctc ctgcggttgc cccgtctccc gcggccgctc
    10621 ccgcccccgc agctgccccc gcctctgcgg ctgctcctgc gggaggtact accgttaccg
    10681 cgccgatgcc cggcaaggtc cttaaggtgg cggtgagcgt gggagccgcg gttaacgcgg
    10741 gagatctcct tctggttctc gaagccatga agatggaaaa cgagatacag gctccttctg
    10801 ccgggacggt caaggagatc aaggtctccg acggatctcc ggtcaacacc ggcgacgtgc
    10861 tcgtggttct gggctagggc ttcaataagc ccttgccagg ggaggtacgc aaatgggact
    10921 ttatttcagg gctcttgcgg agatcgcgga acaatcggga ttcgcggggc tgacaaccgg
    10981 gaacctgata atgctggtgg tggcgctggt tcttttgtac ctggccattg ccaaggggtt
    11041 tgagccgctg cttcttgttc ctatagcaac aggatgcctg ctcgttaacc tgccactttc
    11101 ggggatcatg gatgaaggcg gatttctccg ttacctgttc ttcggcgccg agcacgagat
    11161 ataccctgtg attatattca tgggaattgg tgccctgacg gatttctcgc cccttcttgc
    11221 gaaccccatg acgttccttc tgggggctgc tgcccagctt ggagtgtttg tagcactttt
    11281 cggtgccatg gtgctcggat tctccgtccc cgaagcggca tccattgcca ttatcggagg
    11341 agcagacggt cctacatcca tataccttac gatgaaactt gctccacaga tccttggggc
    11401 ggtagcggtt gcggcctaca gttacatgtc ccttgtaccg cttatccagc ccccggtcat
    11461 caagatgttc accacaaaaa aggacagggc tatcaggatg gatgccctgc gccccgttag
    11521 caagagggag aggatacttt tccccatcgt ttgcacggta tctgcaggac tgatacttcc
    11581 tgcgtcggtt ccccttatcg gtcttctgat gttcgggaac cttctccggg agtgcggtgt
    11641 cacagaacgt ctcaaccagg cggcccagaa cgagatcctc aacactgtca ccattcttct
    11701 ggggctttcc gttggagcca ccatgggcgc ggagaccttc ctgaccgttg ccacgatcaa
    11761 gatcatcctt ctgggtctgg tggcgttcat cttctccacc gcggggggaa ctctgttcgg
    11821 gcagttgatg aaggtccttt cgggaggcaa gatcaatccg atgatcggag cggcgggagt
    11881 ttcggctgtc ccgatggcgg cgagggtcgt gcagaggata agtgcccagg agaacccttc
    11941 caatttcctg ctaatgcatg ccatggggcc caacgtggct ggggtcatcg gtacagccgt
    12001 tgccgccggt gccatgctga cccttctgac gaactagaat cgtcccttcg cgcgacaaag
    12061 tcagtaaaaa gaaaagaggg agcggattcg gcctggccgg gaacgttccc tctttttttc
    12121 ggtgtgaatg aaggggccct cctgtgttcc gaagccttga agaaggtctc tctttggggt
    12181 aaaaatgtat gggtaataca atatgctgtc cagggggagt aaatgtgaat ctttcaatgt
    12241 tcctttccaa tgaggtgaaa cctgcgctgg gatgtaccga accaggggcg gtggctcttg
    12301 ccgcctcctg ggctgctcga tactccgaag gtgatatcaa ggggataaaa ctgcttcttt
    12361 cccccaatat attcaagaac gggctccatg ccggtatacc gggaacgggg ggcgagaccg
    12421 gcaaccttct tgctgccgca ttgggggcca tgaagggtga tccggataag ggccttaccg
    12481 ctctcgaagg gataacgggt gatgatgttt cgagagctct ggattttgta aaaacaggtg
    12541 gtgttacgca gcaggtcctt caggatgtgc ccgatgtcta tgttcaggcg gaggtcgttt
    12601 cggactcggg tacggtgtcc gtaattatct ccggcaggca cgacaggatt gtggagataa
    12661 ggaagaacgg agatgtcatc aagaggctgc ttgaggccga cacggaaggc ggaggtcgtc
    12721 ctccctatat tgatgatctc ctcgaaaagg acatggagtg gttctggtcg tcggcatcat
    12781 ccatagacgc cgggatggaa aagttcctga tcgaaggctc cagagtgaat ctcaaagtcg
    12841 cggagacagg gctgcaaaag ccctggggtc tggggacggg atatcatctg gcggctctcg
    12901 ggggaatgga cgatcttttg agacaggtca aggcatgggc cgcggcagcg gcagatgtcc
    12961 ggatggcggg cggagcctgg cccgttatga gcagcgcggg aagtggcaac cacggaatca
    13021 cggcaataat ccccccttca ctggcggctc gggtatgggg gaagagcgaa agggaacttg
    13081 ccgaagcact tatgctgagt catcttgtca ccggtttcat aaaggcccac accggccgcc
    13141 ttaccccggt atgtggatgt gccgtagctg cgggcgcggg agcggcggca gccatcgtca
    13201 gacttggtgg tggtaccgca aaccagggag agatagcggt ggctcttttg ctatcatctc
    13261 ttcaggggat ggtatgtgac ggggccaagg gttcatgtgc cctcaaggta agcaccgcag
    13321 ccggggaatc ttttatttcg gctcagttgg ctttgggtgg cataggtgac ccgggagaac
    13381 agggcatcat atcgtcaaat tttgttgaag ccgcccgaaa cgtgggcaat ttcagccgta
    13441 acgggctatc aggggcagat tcagccatta tccgcattct tcaggagatg gaggcttccg
    13501 gaaagaactc ctgagagagc gcagggtctt caggtcggga tcaccaggac tggacagggt
    13561 gatttttcta ttatggcttc cccggtgctc ccgatgatca gttcccagag ttgtccgtgc
    13621 gtggatagcc ctaccacgag catccccggg gtcatttccc gggttttccg gagtatctct
    13681 atctccggtt cgcccgcgag aacttcggct ttaacatcag agttccatga ggccactgat
    13741 tccctgacct cctcaagagc agaagatgct atttggaaca tatcgctgga ggttgaaggg
    13801 tcatcaagag atactgaatg aatgatagtc attggaacgt ttcttccatc gaggaggttc
    13861 ctgagttctt cgagcacccg gtcggtcctc tccggagaaa ggtccgtgca gacggccacg
    13921 tgattgaaag ggttctggaa agggaccttt gtctctgatg tttttacgac caggtgtgga
    13981 atggccatgt atcgtatgaa gttaactact acgtcgttgc tttcagcgaa taagaccgag
    14041 acggaacacc cacgggagtg tgctgtctcc gaaagcgttt caaggggatc tccggacagg
    14101 acaagggaac tgcacttgat ccctgttgtg cccagttcag acacggtctt ctccatggct
    14161 ttttcggctt cctgaatcat tcgggaagta tcaagtcctg tcatgggttc tactacatgc
    14221 acaaggacga tctccgagga tccatcggct acattggcgg ctgtccatag aaggcccttg
    14281 tgggacagtt ctgaaaaatc tgttgggaag agaattttac ggaacatcac gatagacccc
    14341 ctttagtttt gtgctagact aaatcagttt atcagacaca accgggcagt caagaaaaaa
    14401 cctgatcttg gatggaggac gcaaattgag cccatcgccg aattttacct ggacagtact
    14461 ctcgggtttt gccaggattg tcagcatcct tccccacagg gtttcggtct ctattggggc
    14521 ctggacaggt ttggtcgtat ggtttttcag ccggtcaagg gtggacagag ccgaggcaag
    14581 atgcgtaaag gctctgcagg tcggggttac aagggctcgc gggatagtaa gggaatccta
    14641 cgtgaacctc ggcagaagtc ttgcggaatt tctgagactc ccggtgcttg gtaaaaaggt
    14701 tctggattat gtggatatac atggcgagga caacctcaga atcgcactgg aaagggggaa
    14761 gggcgtgata ttccttacgg cccacttcgg gaactgggag tataacgcct ccaccatggc
    14821 cgtaaggggt ttccccatga acgctatagg ggcagagcag agagatccca ggatcactga
    14881 acttgtcgtg tcgctcaggt ccgcctgtgg tgtcaagact atgagcaagg gcttcgatct
    14941 caaaagcgcg gtgcgatgcc tcagagaggg ggagatactg ggcatactcc ttgatcagga
    15001 ttttgcggac aacggtatag ttattccttt tttgggaatt ccggcgagta ctcccttcgg
    15061 tccagtgaag atggccggga agataggttc ctccgtagtg ccgactttca ttgttcgccg
    15121 aaaggatgga ataaatcacg atctgtatat tctgcccccc ctggactttg ctggaggcaa
    15181 acccttcggt gaggatgtag aggcgtccct taagctgtgc aacgacaccc ttggcgaatg
    15241 gataaggaaa caccccgacc agtggatgtg gctatacccc cgttgggcat caacgacagg
    15301 agacaggtaa ttgatcgcgg ccgtggatcc gggaagggag aagtttgggt gggctatctg
    15361 tgacgaagca gggcaacttt tcctttcggg ggtttctccc gtcggagagg tctgtcgatg
    15421 gggcggggct cttgcggcgg cggattatga atacctggag agcattgccc ttgaaaaaag
    15481 aggacccttt cagattgaaa accgtcagtt cctccttctt gcgggttcgg gaaccgggag
    15541 cggtcatgta attgaaatgt tgcggcagac aggtctcgag gtatttgctg ttccagagga
    15601 aaattcgacc ctcaggggaa gggccctttt ctggaagatc catcctccga aaggcctggc
    15661 caggcttctg cccagatctc tcgcggtacc atggcgttgt gccgatgata tggcagcatg
    15721 gtgcctggtt cttgaacatc tggaaaggca gagatccaaa aatattgata gatccggagg
    15781 ataacggtca tgtcatattc ttcaagactc aaggtttaca ttgaacagcc cgggttttca
    15841 gtgggatgtg ttgacctgga gtacagtgag gaggaaatga agagtttccc catggcttca
    15901 ggacgtcttc aggtcgggaa gatcctggta aaacttcgcg aaaaatacgg cgacgacctt
    15961 cgggtcgatc tgatagaccc cagaagtctg actgcaatgc tggacatact caaatacagg
    16021 atcaagagca ccgagcctac ctggatattg gacggaaaac tgatcttcag gggggttccc
    16081 gaatgggagg aacttcaggc aataattgat gcttttcccg gataataaag gaagcagggt
    16141 gttgaggttt gaaaaaatgg ttcagcgatg ttgcaattga ggaactggtt cttggatatg
    16201 cctttgtaaa gtggatcata cttgctgtat ttgccggtct aattgtgggt tccgcggctt
    16261 cgttgtttct cctgttactc gataaagggg ttgccggggg gttatctttg ggtccggcca
    16321 gattgttgct tctccctcca ggacttttgg ccagcgtctt tctggtcaga aaaatggctc
    16381 ccgatgccgc gggacatggt actgaaaaag ttatagaagc gattcaccag aggtctggca
    16441 ggatatcact tcccaccgtc cccgtaaaac ttgttgcgac tgtaattact atcgcatcgg
    16501 gtgggtccgc cggaaaagaa ggcccatgtg cccagattgg agcgggactt acttctgcca
    16561 tggcaagcct catggggctt aatgatacgg acagggaaaa acttgttgta tgtggaattt
    16621 ccgctggttt ctcggccata ttcgggaccc ctgtggccgg ggccatattt gggctcgagg
    16681 ttctttttat tgggaaaatg tactacgacg tcatgctgcc tgcctttatc agcgggatga
    16741 tctcctttca gaccgcgtca ctgcttgggg ttgtgtacgc cgaacagaaa ctttcctcgt
    16801 tgacgaccct gggggggaca accttgttgt gggcgactct ggcgggtgtg ttttttggat
    16861 tagttgccct tgcacatatc gagatcgtat cggcttcaga taaatggata agcaggatac
    16921 cagcagggcc ctacatcaag gcggcaggag gcggtgtggt tctccttgtg ttggccatgt
    16981 tatttggaac ccgctacctg ggcctgggga ctgattttat tgaggaggcc atttctggtt
    17041 cgaatgttcc gggaggagcc tttcttctga aatctctgtt tactggtata actctttcgg
    17101 cagggggtac cggagggata ttgacgccga cctttttcgt aggggcggga tcgggctctt
    17161 tttttgcatc cctgttcggt cttgatggag cgcttttcag ttccatcggt ctggtagccg
    17221 tacttgccgg ggccgcaaat acgccgatct ccgcgacgat catggcgata gaactttttg
    17281 gttccgaagt tgcttcctat gctgccatcg cctgtatagt ctcttttgcg attagtggtc
    17341 acagaagcat ctatccaagc cagatcctgg caaggccaaa atcatctgtt ttcggggttt
    17401 cgaaagatgg tctccctgtg gaatttcacg aaaacaggat caatctttcc gataccagga
    17461 tcccttcgat gcttttagag gcaaaagcat attgggagag gcgggttttc ttccccaggg
    17521 acatggagtc accggctgag gtcaaacaac cgaaagatgt tcaggaggga gagtagatcc
    17581 tttcaaggca tgccagtgcc agagaatcga ggctggatat aattttttcc tgatccattg
    17641 ccgacgaacg gtatgcaaga gggagttccg tacaggctcc aataacggga atatcttttt
    17701 ccgaccacag tatcctgaga caggattcca gtctggccgc cgactcactc agcctgttgg
    17761 ctttcaccag ggctattacc tcctccagca tattctgtgt ctcaggtccc ggtataagaa
    17821 ggggataatc ccgttttttt gcttcggtct cgtatatcct cgaagaggaa gtgccgctgg
    17881 tcgctgtcag ccaggctccc ccggggcttg ttctcattgc cctggatatt gtctcctcga
    17941 caatatgaac cagggggacc gacagccggt ccctgaatct gttgatgaaa acatgagccg
    18001 tattgcacgg aacagcaagc aactgagccc cccatgactg tagcgttttc agtccttcca
    18061 gaagcagagg ggcgggatcc tccccgcccc ccgtaattgc cgcggtcctg tcaggtatct
    18121 ggggattcga gaacagtact atccttggat ggtcctggtc tttttcggca ccggccattt
    18181 ctgccagaag acgaaggaat tccgccgtgg cggcaggtcc catacccccg aggataccaa
    18241 ggactttatg cggctttttt gacatccgcc agttcgccct ccgtttttgc gacgaccgtc
    18301 gctacagtgg catcgccggt cacgttgatg gcagtacgag ccatatcgag gacagcgtcg
    18361 atccccgcta tcagagccac tccttcaacg ggaagtcccg cctgggtcag taccagggtc
    18421 agcatgataa gaccggctcc gggaacacct gccgtgccga tcgatgccag tgtagctgtc
    18481 agaagtattc ccagctgggc tccgatcgaa agaggaatac caaaggcctg ggcaacgaag
    18541 agggcgcaga caccctggta gagagccgtg ccatccatgt tgatcgtagc gccgagaggc
    18601 agcacgaaag aggagatctt ctcggaaaca ccgatgtttt cctgggttac cctcatagta
    18661 atggggaggg ttgcagaact tgaacgtgtg acgaaagctg tcagggtggc ctccttgacc
    18721 ccgtcaaaga atttccaggg tgacatgtga actatcgaga cgaggagacc ggagtagacc
    18781 agaagtgcgt gaaggatgca tcccaggtag accccgaata tgaccttgcc gaagggagca
    18841 aggacagaga caccgtattt tgctaccgtt gccgctatca gggcgaaaac gccatagggg
    18901 gcaacatgca taaccagtcc ggtcattttg tacatggtct cggccacaga atccattaat
    18961 ccgataaggg gcttcccttt ttctccggcg aagaccgctg aaattcccag gaaaagggcg
    19021 aagacaatta tctgcagcat gtgtcccgac gccatcgatg atatcgggtt tttggggaag
    19081 agtcctatta tgacatcaga ggtagaaggc ggcgttggaa ccttgaaccc tgaaaggtcg
    19141 gctccgagac ccataccgac tcccggctgt atgatgtttc ccagtaaaag ccctatagcg
    19201 atagccacgg ccgtggttgc cagatagagc ccgatggttt tgataccaac tcttccaagg
    19261 gctcggggat cgcccagtga tgcggtcccc actacgaggc tggaaaaaac caggggaacg
    19321 acgagcatct ggagtagagt gacgaagata gtcccgacgg gtgctataaa cgaaatagaa
    19381 tctccgaaaa ctattccagc caggataccg gcgacgaaac cgatcgcaat cttccagatc
    19441 aggggcattt ttttcttctc tgccaaaata tcccactcct ctcaattatt tcaaggtgcc
    19501 ggatgaaacg gttcatctaa aacgacatca tccctcccgc cgatcacctc cgggtgaata
    19561 ttccgggaaa ggtagatata aaagaggaaa ttaaatgaat cgctgacaag attatatctc
    19621 aattgtctct ttttgtcttc ggcactgtgc ttgggaagcg ttctatctgg taatatggtc
    19681 tggaaactta ttcgtccagc cacgggaggg gtctttcttg cgcagggtga ttatgcgagg
    19741 ccttatagtc atgcttctta tcgctcttgc cgcttcggga tcatacgcgg gcggggtggt
    19801 tctggtgctt tcggggggag gaacccgggg acttgcccac gtgggagtcc tcagggtcct
    19861 ggaagataac ggtatcagtg tcgaagggat cgtggggaca agcataggct ctattgtggg
    19921 gggtctggcg gcctgcggct attcatctgg agaactcgaa gacatgctgg ccggcctgga
    19981 catgacctcc ctgctatacg acagacagag agaggcccaa tctcctccgg gtgaggaagg
    20041 gacagattca tccctttccc tgttccggct tgagttcagc gaaaggggac atataaccgg
    20101 acctctcggg ggattgagcg ggaagagact tcttgagaga ctccaggaat ggacggttcg
    20161 ttgcccggtt ctcgattttg ctgatctgcc agtgcctttc gcggcagtag cgacggatct
    20221 tgtgaccggt gagccggttg tactcagaag aggaaatctt gcctccgcca tgcgcgcatc
    20281 catggctatt ccaggcctct tttctccatg gaacgtggac ggcagactcc tggtggatgg
    20341 aggccttgtg tccaacgctc cggtgctggt tgccagagaa ctcttcccgg ggtatccggt
    20401 ggtggtcgtc gacgtaagcg gtaagggcaa gagcagggat gacattcgaa ccgttgtgga
    20461 tgtaatggat cagatgatct ccatcatgac catgcgaaat gtgcaggaag aacttaaaca
    20521 tgcggatctc gttataaccc ctgatgtagg gggacttccc atgctggacg tggcaggaca
    20581 cgaggagatc ataaaaagcg gggaacttgc cgcgagggac aaacttgacc agattatatc
    20641 cgttgcctca aaaacgaccc cttccagccc cctcaaagca agtgaaaaaa ttattgtcgc
    20701 cgaggtcaga accacagggc ttggagaaag gttctctcaa gcggttatcg acaggtatga
    20761 cctttgggta gggaaagaag ccgaccccga atctattatt caggcctgcg ttgacctgag
    20821 acaaagagat gatgtcaaag cggcggattt ctccctcgaa tatctaccgg atggcagggc
    20881 tgccgtggtt ctgaatgtcg aaaaggaacc agcctgggaa gttgttgctg gaggatatgc
    20941 ctccaatctc aacccttatg ccgcggtcta cctcgatacg atcagaaggg atctcttttc
    21001 ggaaggagat tctctaagga cccgcttcgg gatcagcgaa cgctggcatt tctcggcgag
    21061 atacctttct cccgtcgagg agggttacgg caggtgggag gcaaacatca agggaggcaa
    21121 aaggatctac tctccaccag gatccgggac actggagtgg gagcagtatt ccgcttcatt
    21181 gtggcggcat tatggtgacg gtcctttcag ggcttctctg ggttttgccg tggaagagac
    21241 aaggtcaggg gggcagacca acacttacac cggtcctgtt ctctctcttg cctttgaagg
    21301 cctggatgac ccgatagacc ccactgaggg gttttcggcc tccttgtcac tatggtggcc
    21361 cgatactgac gaactcctcg gaagaatgcg tttcttcggg gtaacgagtt cagcaaagga
    21421 ctggagattc tatatcaggg gcggagccat ccttggagat ggaagcaggg catggcattc
    21481 ggcatatctg ggagcaaggg aggaacttta ctccaggggg gcaagcccac tccgttcttc
    21541 caatgctgcc tgggcgggtg tagggataag gaaagttttc ctcaagagct ggtgggggac
    21601 aataaacctg gacctgttcg cgacagcggg gagaacatac gactcatcct ggagtggtgt
    21661 tgacaaaatc tgggagacgg gcatcgcggt ctctcttccc ggaaaaattt tcgatggcaa
    21721 gattcttgta atctatgatg accggtcgga ctggagcttc gggtttgttc ttgggagacc
    21781 gctttgggaa aacgacccgc ttccatgata tccggcttgc cccgggaggg gctttatgac
    21841 tacaatgtgc gatatgtcgt aaaagaggct gcttaaggac acgcaaacgt attgaggagg
    21901 aagagaatat ggctcgcaat attgctccaa gagaaaagga ctattcccag tggtatctcg
    21961 atgtgatcaa ggcggcggag cttgctgatt acgcgccggt ccggggttgc atggtcattc
    22021 gaccggacgg ctatgccctc tgggaatcga tacagcattt ttttgacgag gctttcaagg
    22081 atagcggcca cgtcaacgcc tatttcccgc tcctcatacc gatatccttc cttgagaaag
    22141 aagcggatca tgtggaaggc ttcgctcccg agtgcgccgt ggtgactcat gcaggaggag
    22201 aggaacttga ggaagcgctg atcgtccggc cgacatcgga gaccgtcatt ggccatatgt
    22261 acagtaaatg ggtccagtcc tggagagacc ttcccatcct gatcaaccag tggtgcaacg
    22321 tcatgagatg ggaaaaaaga tccagacttt tcctgagaac ctccgaattc ctctggcagg
    22381 aggggcatac ggcgcacgcc tcacgcgaag aagcagttga ggagacactg cgcatgctcg
    22441 aaatatacag ggacataatg gaaaacgtcc ttgcgctgcc ggtcctggcg ggggagaaat
    22501 ccgagggaga acgtttcccc ggtgccgaca acacctacac ctgcgaagcc atgatgacag
    22561 acaggaaagc tctccaggcc ggaaccagcc actttctggg acagaacttt gccaaagctt
    22621 tcgagataaa cttccagaac agggaaggag aactggagag cgcatggact accagctggg
    22681 gtgtttcgac gaggcttatc ggcgcgatag taatgaccca ttccgatgat gatggcctgg
    22741 tgataccccc ccgggtggct ccggtgaagg ccgtgatcct gcctatcagc cccgaccagg
    22801 agatggtcga gggcaaactt cttcccaggg ctgcggagat cgccgaaaaa ctcaatgcgg
    22861 ttcttgggaa aagatccgta aagatcgaca aaaatttcca catgaggccc ggagaccgtt
    22921 tctttgcaag tcttcagaag gggatccccc tgaggctgga gctcggagaa aaggaatttg
    22981 gaaaagaagt tctccatgcg gtccggaggg acacagggga aaggttcgac atcccatggg
    23041 atgaggtcgc gacaagggtt ccggaagtac tggaggatat acagaagaac ctctacgaaa
    23101 aggctcttgc cttcagggaa aagaacacaa ttcatgtaac tggttacgaa gagttcagta
    23161 aaatatttga aggcagcgga ggtttcgtgg aggccttttt cgctggcggg cgtgaagaag
    23221 agaaaaagat aaaagaggag acaggggcaa cggttcgctg ttttcccctt ggaagggaag
    23281 aaacagggac ctgtttctat acaggcaaaa aaggagcccg gctggctgtt ttcgccaaat
    23341 cgtactgatc ttttgatgaa agaacttgtt cccgtggagg tcgccagagg gtaaaatcga
    23401 aagtggaagg tctgcgcacc tgcgccggaa gcatttaatt cattcccgac agggaggaga
    23461 gaacatggtt tccgaacttg gtgtcggaaa taccaatatc cgtctttaca aaggggatat
    23521 taccggattt caggg
//
LOCUS       DAOTIQ010000025        23440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2551_length_23440_cov_7.306521, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000025 DAOTIQ010000000
VERSION     DAOTIQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23440
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_23440_cov_7.306521"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1312
                     /locus_tag="P5541_04800"
     CDS             125..1312
                     /locus_tag="P5541_04800"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRV71331.1"
                     /translation="MSWETKRLEAVADFCLGKMLDQKKNRGEPLPYLANVNVRWSEFD
                     LCDLREMPFEHHELERYGLRYGDIVMCEGGEPGRCAIWKDQRSSMMIQKALHRIRPHE
                     CLRPEFLYYFFLYRGRTGHLSPLFTGTTIKHLPREKLALVEVPVPPHSAQDSITAVLS
                     AYDDLIENNRRRIQLLEESARLLYKEWFVHLRFPGHEHVKITDGVPEGWERKCLGNLC
                     REVRETVLPKELEPKTPYIGLEHMPRRSISLREWGCVEQVVSSKHRFRQGDILFGKIR
                     PYFHKVGAALVDGIASSDAIVIRANEENLMPIVLMTVSSDAFVALTSQKMKEGSKMPR
                     ADWKQMKQYPVLLPQESLLGLFNSVISPILEQPKSLVFSNRKLIEARDLLLPRLMNGE
                     VML"
     gene            1312..5103
                     /locus_tag="P5541_04805"
     CDS             1312..5103
                     /locus_tag="P5541_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRV71332.1"
                     /translation="MPAFVANGPDIPERLLQEHEDGRVVFFCGAGISFPAGLPSFDGL
                     VKAVYLNFGGPQNEVQQAAIKAKQFDTAIGLLEDDIGDRKVVRDAILRILTPKTITSK
                     VLETHEALLTLSQNRDGRRRLITTNFDRLFEEVIARKKLVFESFKAPLLPIPKNRWDG
                     LVYLHGLLPSEPNTSEFDRLVVSSGDFGLAYLSERWAARFLSELFRNYIVCFVGYSIN
                     DPVLRYMMDALAADRLLGESPREMFAFGSYPKGKEEQAFKEWKAKTVTPILYRKYKGH
                     EYLHKTLREWAKTYRDGVQGKKMIIAQHASTPPLAPTRSDFAVGRVLWALTDKSAAKH
                     FAELNPVPPIEWLEPLSEPQFKHDDLSRFGVVPEREKNDELAFSFICRPSPYLLSSFM
                     HISDRGYRNDRWDGVMFQIASWLTRHLDDSKLILWLANQGGQLHPFFMDLVKSKIEEL
                     DELERSGENKKFNHVKNKSLKGIPSLQMRTLWDLFLSGRIKKKNHTLDIYDWFRHFKH
                     SGLTPVLRMELREFLSPKVNISAPIPWPELGISEESDSIKKLVDWNIELSSDDMHTAL
                     ENWSRKPEWHEVLPNLLNDFTSSLHDVLDLMQGLDGAGEKSDLSYIHQPSISEHPQNK
                     AFRDWTILIELARDAWIATAQTNRTKARLAAENWWQISFPLFKRLVFFASTYDDVVEK
                     RQALEWLLTDEQWWLWSLETEREAIRLIVALSSKWNVKEMGKLEQAILLGPPRSMYRE
                     DIKPERWERIIDREIWLRLAKIQNAGGLLSNGAANKLAKLIDKYPKWRLAENESDEFP
                     FWMGMGMEDEEWRQFVRVPRRRRKLVEYLKESSQSDSWREDDWNELCREKFSTTACAL
                     YSLAKEGKWPVKPWKEALHVWKEGKLGTKSWRYLAQLIYIMPDNIIQELSNPLGWWLQ
                     AQANNFQGQDEVFLGLAKKILDLEYELEEDPKKDDLVFESINHPIGLVTEALLDWWYR
                     QEPKDGQGLTEDLKNIFTMLCDTSIPKNIRGRLILTGHAISLFRVDKEWTSTYLLPLF
                     DWTISKIEARGAWKGFLWSPRIYRPLLSAIKRPLLETSTHFTSLGEHARQYASFLTFL
                     ALDSANDFSNRELGNATRTLPKEGLESVVRTLVRALEGSDEQREEYWSNRILPYLRNI
                     WPKSRDLNTPTISESFGRLCIAAGGGFPEAFNELQNWLKLIHGPDFLIHLLFEAKLHE
                     HFPEETLGFLNLIISDDAQFLPEELIQCLNEIKSSSEELSGDARLDRLYSLYERHGKS
                     G"
     gene            5364..6776
                     /locus_tag="P5541_04810"
     CDS             5364..6776
                     /locus_tag="P5541_04810"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV71333.1"
                     /translation="MNRKSLADLIALGEGFTTEFKKAGTSNLGREICAFANATGGVIL
                     IGVTDLGDIVGVDNHNRLKSEIQNIAHSAEPPIAVEVESTGKVLCINVPQQHSKPYSF
                     GGKFFMREGSSSQQMSHQEIREFFFKEGLIHFDETPCTRFSLKNDLTEEAWSVFRKRA
                     RIPEGMDTMVVLENLHLVRDGQMTHAGAWLLASDIQKFNISADVACALFMGVDKVRIL
                     DRRGFNSDVYSMIDEVVTYILSKINVEYIIKHVKREERPELPEEALREAVVNALAHRD
                     YRSTANVQVYIFKDRIEIVSPGGLPAGMTEADLGIKSIPRNPLLFAMLHRMEAVEHIG
                     SGIKRIRDLCREYGVAEPRIEVSEHWFTMIFPRPGVEPEAELGFDTPHDTPHDTPHDA
                     PQVVKLAEALVGEMNREEIMIALGLKDRMHFANYYLKPALYSDVIEMTIPDKPRSKNQ
                     RYRLSAKGKLLLASREKSRK"
     gene            6773..10027
                     /locus_tag="P5541_04815"
     CDS             6773..10027
                     /locus_tag="P5541_04815"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRV71334.1"
                     /translation="MTPIPYTEDTLVQQTTAEYLEKQLGWESVYAFNNEDFGPESLLG
                     RATGRDVVLTRYLRSSMEKLNPGLPQTAYDDAVRQIVTIAATQSLVATNSDKYSLVRD
                     GVKVTFRNEKGERVRQLIRVFDFNEPSNNHFLCVRELWVRGDLYRRRADIVGFVNGLP
                     LLFMELKNVSRDIRAAYEENLKDYRDTVPHLFHHNAFIVLANGVDAKLGSVTSRFEHF
                     HEWKRLAEDQPGAVEMETLLKGICDKHNFMDLFENFIVFDDSSGEKKKILARNHQYLG
                     VNRAVDAVQNRKSLKGRLGVFWHTQGAGKSYSMVFFTRKIHRKLGRNFTFLILTDRDD
                     LDKQIYDTFAGCGVVNNDREPCRASSGGHLSRLLAQQKSHIFSLIQKFNLEVDPDEGY
                     TQRDDIIVITDEAHRTQYGTLALNMRNALPNASYIGFTGTPLFKDDEITRRVFGGYIS
                     TYDFQRAVEDKATVPLYYDARGEKLGVAIGDVNERIAEKLEEFESEDIDVQQRLEQEM
                     KRDYHIITAGKRLDQVARDFVKHYSGAWETGKAMLVCIDKITCARMYKLIAFYWDQRI
                     KEQEKSLLKAEGEQEEAYLLRQIEWMRQTRMAVVVSEEQGEVEKFRKWGLDITTHRRL
                     IKEGMDLPEEMRRLPQYHTIQSLSLDDAFKEEEHPFRIAIVCAMWLTGFDVPCLSTLY
                     LDKPLKAHTLMQAIARANRVNEGKENGMIVDYCGILKNLRKALATFAGAGDGGRGKEG
                     GEIEPARPDGELLDDLAESIALVCAFLSEYGTSLEGIISNSGFERNAAILACKEAANS
                     NDETRKRFEVMCRAIFSKFKACINVQGINTHRARHDAISIVYRSLQQDREKADITDII
                     RSLQKVIDEAIVTKPERVASGSAPYDISRIDFDRLKKEFARSPAKKTTVQNLRQLVEN
                     RLQRLLEQNPLRTDFQRHFEEIIAEYNNEKDRVTIEKTFEELLRFYEDLNEEESRAVS
                     EGLDEESLAVFDLLRKPDLTASETNRIKKISVDLLENLKAEKLRIDHWRDKEATRDAV
                     RLSISDFLWIESTGLPVDRYSEEDVKKRAEEVYLHVFRVYPVLPSPFYQAQTVA"
     gene            complement(10416..14012)
                     /locus_tag="P5541_04820"
     CDS             complement(10416..14012)
                     /locus_tag="P5541_04820"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRV71335.1"
                     /translation="MTSASSQYTAKDIQILEGLEAVRRRPGMYIGDTGPRGLHHLVYE
                     VVDNAIDEAMAGWCSHISITIHSDGRISIEDNGRGIPIDVHPQTGRPAAEVVLTTLHA
                     GGKFDHKSYQVSGGLHGVGVSVVNALSEWLKLTVWRDGKEWRQSYNKGIPLGDLCCGE
                     STEKNGTLIEFLPDSEIFEEVIFSWDTLSGRFREMAFLNPGLAIKLKEESTEKEKIFH
                     YEGGISSFVEYLNRGKNILFKPPIVISGEKDGVTVEIGVQYNDSYIERVFAFANLINT
                     IEGGTHVSGFRTALTRAINESARRNKILKEKDPNLTGDDLKEGLTAVISVKLPEPQFE
                     GQTKTKLGNGEIKGIVDSLFYDGLMADIDERPDILKTVVEKAVRARLAREAAKKAREL
                     VRRKTALAGLDLPGKLADCSSRNPEECEIFIVEGDSAGGSAKQGRDRNFQAILPLRGK
                     ILNVEKARIDKALDNNEIRTIIQALGAGVGDDFNVNNIRYNKTFIMSVDGEEPTLVKD
                     PFGSVRQVRIGEFIDKAIDGSLCPDGYEVMCFDLETGNTGFRPLKKVIRHTIEEPLYE
                     IRTAYGRNVRVTSSHSVFAVDKGRVVLKKGSEIVEGDLLVAPRTIKIEGKGIRKLDIL
                     KELFARKDRLDSNLYVRGKAIEDMLKNEILEEYSDKPELTDRRVEIPEDVRETLKGAR
                     KTCGFTNREICQKTGIRQPITFYGWENGSNRPTVTNFQNYAKAVGLDPDNIMDKVRTT
                     DSNLENVWNKQYRGSCSNRVKPYIRIQDLSPEGVACLEDAGIALTPEHYASRAVPRNI
                     PVDDDLLTILGFFAAEGSLDQRQGIRFAIGSNNEPLRKEVCDRFSRVFGLDLHWYPGK
                     NGRSGELKILNSVLSTVFRLVFGFDGARSFSKRVPDIVFNTDKNLQKAFLRGYFLGDG
                     TIRDSGISVTTTSKDLASGVLFLLSSLDIVATLHSREPSDTEGMIRSKPVRTKHRYHT
                     ITVSALDDLRKIEPVWQDHRLAGKLWEKINNGSSKNKNRRFVPLQGDILGLKVLEVKE
                     VEPSTRWVYDFSVEKDENFICGMGGICCHNTDADVDGSHIRTLLLTLFYRFMPELIEK
                     GHLYVAQPPLYRTQTGKEVNYCYSEKELKAIQDSAKGKKLTIQRYKGLGEMNPDQLWE
                     TTMDPATRICKRVEIEDAVSAEEYFSILMGDKVEPRREFIEAHAKEVRNLDV"
     gene            complement(14009..14221)
                     /locus_tag="P5541_04825"
     CDS             complement(14009..14221)
                     /locus_tag="P5541_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71336.1"
                     /translation="MFLLLEESMAVHTGKVVALVRFGGETAVLMRDGSVMATGFTPAT
                     LARRSRNFMKKGKERARDLRQGGKIL"
     gene            complement(14302..14937)
                     /locus_tag="P5541_04830"
     CDS             complement(14302..14937)
                     /locus_tag="P5541_04830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRV71337.1"
                     /translation="MDEWTRPDPSRMAMLTIDIQREFQPGGSSGREENARTVPAAAHL
                     AKAFRRAAKPLIHIVRLYLPDASNADLCRRSRILSGEPLLVPGSVSSQIAAPLLPDPS
                     TLLDHRLLLSGGIQKIGPSEYIIYKPRFGAFFGTPLMEHLEALGVDTLAVTGSNYPNC
                     PRATIYEACDRDFRVIAVTDGISLLNEAAKRELEAIGVNCLSSAETVSLLP"
     gene            complement(15039..15512)
                     /locus_tag="P5541_04835"
     CDS             complement(15039..15512)
                     /locus_tag="P5541_04835"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRV71338.1"
                     /translation="MKEIGSMAPDFCLRDSNGDEKCLQEFRGKWVVLYFYPKDNTSGC
                     TLEAMEFSALEDEFSRNKAVVIGVSPDTCESHARFIGKHNLTVILLSDPEKKVLTGYG
                     AWRMKKMYGKENMGVVRSTVLISPEGEIARTWDNVRAAGHAQKVLDEISTVNSGS"
     gene            complement(15533..16309)
                     /locus_tag="P5541_04840"
     CDS             complement(15533..16309)
                     /locus_tag="P5541_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRV71339.1"
                     /translation="MKSIRLFLFLLLPMMLLSMPRETLFAEDLPWIEHEVRQGDTLAS
                     ISNKYGMEIYFIRWANELPGEEIPFELTTILVPREENLLIETMSEVRARRNGETTANL
                     YHSEETLPEPEPVKGTAEASPDKNTVRTAEVRPFSLSWPVEGKIYSKYGPRRGRFHAG
                     IDISAPRGTPIYAAAAGTVVRAAWRSGFGRSILIDHNNGVMTRYAHCDTMLCKAGDTV
                     AAGQKIATVGRTGRTTGPHLHFEVVINGKHQDPEKHLPAR"
     gene            complement(16414..20004)
                     /locus_tag="P5541_04845"
     CDS             complement(16414..20004)
                     /locus_tag="P5541_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRV71340.1"
                     /translation="MTATMTEVFREYLENKAALEPGQVEAVMSDSPMIVVSAGAGTGK
                     TLTLAWRFVRLVAVDRIPVDRILTITFTEKAAMEMRERIRRLMALLSSDMPEFCGAME
                     EALSRLDEAYVSTIHSFSMRVLRECGLSVDMDPNIRVISPPEENSFWQFLERAIDRED
                     LDPLTESLDPIWRERGSNLLEENTTADIINTFGAINLSRAASSAIPLFESRNISPEDL
                     WVWADNIVERDKALSRELLDSLVPLWTEAWKVWIEGILPDAGAPEIFKGDKAIFAGRA
                     LDFFKRWNTSPPGKEKLPAFALALMDKEKGLIGNLKGSTGKCMKQVKGSVLKITGEPL
                     AEYRDSRLSWVSAAKWITWGSSPEETLLREKLLRLISLFWKYFESTKLSRGTISFEDM
                     IRGARKAIVGEPSFAGKFRHVIVDEFQDTNSLQDELITSLSPSEGGSLFLVGDLQQSI
                     YRFRHAQPEIFWKRINSPGGSGCRLIDLDVTFRCRQDVMDSINGLFGETWKDGIASST
                     GKKFSPLMPPESRQWWKQRQEISASPFEIIIASDPGSEKPARTPQLREAALRVLADKI
                     VETVSTGATVWREDGAGTFSPGPAAYRDIAVLVPSRGYYGIIEKIFIEERGIPTYFER
                     NRNYFERGEVKDAVSLLQAMADPSDSLALASFLSSPLSGLSPEHASELVSGSIGTNKP
                     LIDWISEKSPDAAEWFDNCRKAGLASGPSVALALLLNESRVLASYPSWRRARVAANLR
                     RAVDLAREYEGAMGRSLSGCAAYLGDVASRGIDSKEADILGEDDDMVRVMTVHSAKGL
                     EFPVVAVTGLERGIRTRGQGTRIIPSAKLGVAATGIPEEWDYECKNDIFGGAIHDLFE
                     TREETEENERLLYVACTRARDSLILCGICPVSKGVPKPKDHSWLRTISSWLGGIEKLP
                     LVRPDEVQPGKTGIRETPEAEQGGSVPAPELPEKPLERISATAYALFRFCPYAFRMRH
                     RQGMDISWESHSGGDGGSDLGSLAHWILKRWDLRPETLPRFDPRVSLSLGSVLPPELR
                     PVWANQSRSIPLIAQLEKFSASPAAERIRCAKDAMKEAPFRVRLKDGTLMVGAIDVVW
                     KSEGRVFIRDYKTGMISGSAETLYDSQLLFYALAARKHFGDAPIDLALVSIEDPGETT
                     IDTRDVSWPDMEDNIISTAKNAGSGPFSPNLAMCPSCPWRRNCSGIHLKP"
     gene            complement(19985..22909)
                     /locus_tag="P5541_04850"
     CDS             complement(19985..22909)
                     /locus_tag="P5541_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRV71341.1"
                     /translation="MPIRIIGYHRARDIEEDLSSSGSKSGLVPFFLVPSSGDREILRD
                     IILESVSFGAGEPAILRWDDLYREVMRELDLPPEERRRQIDPPDHWLIVRHVLENLRA
                     ENDCADLPPGALKRGFIWTLGENLRELLREEVLPDTLGASLGCPSCDPTGKCPRLSTP
                     HGLLCRLYHDYLSYMESHSLADSAQTATITRIALSSHFEKASKWIRQHHFIFAGFMSF
                     TRSQSGLIREISNQGASVTIFMPETGLDIHTAREQFGKMSAPGARPALSPIKALLLSA
                     GDFRLELETVVRNLSLWVSGKGEFARRVDIPFPGWTGIGLSVDPERLEITEEILNRYR
                     VPYVASGGPKVSQTPLWQTAAAALEAKRLGFPTEETSHILSQPWISPDGFPLLKALSQ
                     GPRGEIEWKAFLAVHGDKALSDNFERIADFCALVQSGGSPSELLRSLRSVAGNGEVSI
                     WGAALSRFVMSDPGLDESARRLNAASRELVGKLESVSDMEKDIGPAGSVTLRGAEAST
                     FLSTWADRSTVWQAPKSQESLSLYAGSPPVLARHPVFIVTGLTANEWPGRLRESPLLD
                     DQRKEALHRDPGIGLSPVHLPLLREKRKQREALLRRIIASADDLCILSRPRQDGSGRP
                     LLPSMMLEAAISGDDPWITPTGGDPPFARSMKDVLPSGGETVISNIEIRENDHPRMPG
                     RLSALPPPTPWPVGVAQRASMSSIDTWLECPFKFYAGRVLGLGKTWACGFDPLAAGTM
                     IHALWEKVWTERLSSGADLFCLAERYWEETVHEQYPDLEKLPRHLGRLRKETLKMAHL
                     QQDMENSGLALARTGQKREEKFSITVDGIPFSGRYDRLDILQDGTALLFDYKTGRGNG
                     LSRSLQLAAYAAALRETGSIEISGYIFLSQRDNGVTAILEGNAAGILGKWIKKNQTKL
                     EDMIGSAKKVMEDMALSISESRFIPNYENTQACMFCDFHGLCRKSESTGRGSGEDDGN
                     DD"
BASE COUNT         5477 a         5874 c         6312 g         5777 t
ORIGIN      
        1 aagtggacga gaatttcgac ttcgaggaaa ccctgcgcga aatccatgtg gaactggaag
       61 atctcaacac cgaggctgtg cgactggcgg cggttatcag gaaaaatttt gaggagttag
      121 gggtatgagt tgggaaacca agagactcga agcagtcgca gacttctgcc tcggaaagat
      181 gcttgaccag aaaaagaatc gcggtgaacc attgccatac ctcgctaacg tcaacgttcg
      241 ttggagtgaa tttgatctgt gcgatctgcg tgaaatgcct tttgagcatc atgagttgga
      301 gcgctacgga ctgagatatg gcgacatcgt aatgtgcgaa ggtggtgaac ctggtcgatg
      361 tgcgatctgg aaagatcaac gttcgagcat gatgatccaa aaagcgcttc accggattcg
      421 gccccacgag tgcttaagac cggagtttct ctactatttc ttcctctaca gggggcgaac
      481 cggtcatcta tcgccacttt tcactggaac aacaatcaag cacctccctc gtgagaaact
      541 cgctctcgtt gaagtccctg tgccgcccca ttctgctcag gatagcatca cagctgtcct
      601 ctctgcctac gacgatctga tcgagaacaa ccggcggcgg attcagttac ttgaggagtc
      661 cgccaggttg ctttacaagg aatggttcgt ccacctccgc ttccccggcc atgagcatgt
      721 aaagatcacc gacggtgtcc cggaggggtg ggagaggaaa tgcctaggta acttatgcag
      781 agaggttcgt gagacagtat tgccaaaaga acttgaacca aaaactccct acattggtct
      841 cgaacacatg ccccgtcgtt caatttcttt aagagaatgg ggatgcgtcg aacaggtggt
      901 aagtagcaaa catcgctttc gccaaggaga tattcttttc ggaaaaatcc gtccatactt
      961 ccataaagta ggagctgctt tggtagatgg aattgcatcg tcagatgcca tcgttattcg
     1021 tgcaaatgaa gaaaacttaa tgcctatagt cctcatgacc gtttcttctg atgcttttgt
     1081 agccttaacg tctcagaaga tgaaagaggg ttcaaagatg ccacgggcag actggaaaca
     1141 gatgaaacaa tacccagttc ttctgcctca agaaagcctt ctgggtctat ttaacagcgt
     1201 gattagtcca attctggaac aacctaagtc acttgtattt tcaaacagaa aacttataga
     1261 ggctcgcgac ctgcttctgc cgcgcctgat gaacggggag gtaatgctct gatgcctgcc
     1321 tttgtcgcta acggtccaga tattcccgag aggctcttgc aagaacacga ggatgggcga
     1381 gttgtctttt tttgtggcgc aggtatttct ttccctgcag ggcttcctag ttttgacggt
     1441 cttgtaaaag cggtctactt gaattttggg ggtccccaaa acgaagttca gcaggccgcg
     1501 ataaaagcaa agcagtttga tacagctatc ggtctgttgg aggatgatat cggtgatcgg
     1561 aaagtagttc gcgatgcgat attgcgaatc ctgacgccaa aaaccattac ttcaaaggtt
     1621 cttgaaacgc atgaggcatt acttaccctt tcacaaaatc gtgacggacg taggcgatta
     1681 atcactacca actttgatcg actattcgaa gaggtaattg caaggaaaaa gctcgttttt
     1741 gaatccttca aggcaccatt gttacctatt cccaaaaatc gatgggacgg attggtctat
     1801 ttgcatggcc tattgccatc tgagccaaat acaagtgaat ttgaccgcct cgttgtatcc
     1861 agcggtgatt ttggccttgc ttaccttagc gaacgctggg cagctcggtt cttaagcgag
     1921 ctcttccgca actacatagt gtgttttgtt ggttacagca tcaacgatcc tgtattgcgt
     1981 tacatgatgg atgctctggc agcagatcgt ttgctggggg agtcgccacg cgagatgttt
     2041 gcctttggca gttatccaaa aggcaaagag gagcaggctt tcaaggagtg gaaggcaaaa
     2101 accgtaactc caattcttta tcgaaaatat aaggggcatg agtatctgca taagactttg
     2161 cgcgaatggg ccaagaccta tcgcgatgga gttcagggca aaaaaatgat tatcgctcag
     2221 cacgcaagta cgcccccact agctccaacc agatcagatt ttgcggttgg acgcgttctg
     2281 tgggcattga ccgataagtc agctgctaag cattttgcag aactaaatcc agtgcctcct
     2341 attgaatggc ttgagccact ttcagaacca caattcaagc acgatgattt gtcccgtttt
     2401 ggagtggttc ctgaaagaga aaaaaatgat gaacttgctt tcagcttcat ttgtcggcca
     2461 tcaccctatt tactctcgtc atttatgcac atttccgaca gaggttaccg aaacgacaga
     2521 tgggatggtg ttatgttcca aattgcaagt tggctcacaa ggcaccttga tgattcaaaa
     2581 cttattctct ggttggctaa tcagggaggc cagttgcatc cgttttttat ggatctggta
     2641 aaaagtaaga tagaagaact tgatgaattg gagagatccg gagaaaataa gaaatttaat
     2701 catgtcaaaa ataaatcctt aaagggaatt ccaagtctac agatgcgcac cctttgggat
     2761 ttatttctct ctggccgaat aaaaaagaaa aaccacactc ttgatatata tgactggttt
     2821 cgtcatttta aacactcagg cctaactcct gttttgagga tggaactaag ggaattttta
     2881 tccccaaagg ttaatatcag tgcaccgata ccttggcctg agttgggtat tagtgaagaa
     2941 tcagacagta taaaaaaact ggtagattgg aatatagagc tgtccagtga tgatatgcat
     3001 accgctttgg aaaattggtc gagaaaaccg gaatggcatg aagtgttgcc gaacttattg
     3061 aatgacttta caagctcatt gcatgatgtt cttgatctga tgcaaggatt ggacggcgca
     3121 ggtgaaaaga gtgatttatc ctatattcat caaccttcga ttagcgaaca tcctcagaat
     3181 aaagctttcc gtgattggac aatcttgatc gaactggctc gtgatgcctg gattgctacc
     3241 gcgcagacca acagaactaa ggctcgactg gctgcagaaa attggtggca aatctccttc
     3301 ccgctattca agcggctagt attttttgct tccacatatg acgatgtagt tgaaaaacgt
     3361 caggctcttg agtggctttt gactgatgaa caatggtggc tatggtcctt ggaaacagag
     3421 agggaagcga ttcgactaat agtggccctt tcttcgaaat ggaatgtaaa agaaatggga
     3481 aaattggagc aagcaatttt gctggggcct ccgcgtagca tgtacagaga agatattaag
     3541 cccgaaaggt gggaacgaat tattgatcgt gaaatatggc tgcggctagc aaaaatacaa
     3601 aatgccgggg ggctccttag taatggtgct gcgaataaac ttgccaaatt gattgataaa
     3661 tatccaaaat ggcgacttgc agaaaacgag agtgacgagt ttccattttg gatgggcatg
     3721 ggaatggaag atgaagaatg gcgccaattt gtacgtgtgc cccgtaggcg gcgcaaattg
     3781 gttgaatatc ttaaggaatc ctctcaatcc gattcttggc gggaagatga ttggaatgag
     3841 ttgtgccggg aaaaattctc tacaacagca tgtgctttgt attcacttgc aaaagagggg
     3901 aaatggccgg taaaaccttg gaaagaggcg ttgcacgtct ggaaggaagg gaaacttgga
     3961 acaaaatcct ggcgctatct tgcccaactg atttacataa tgccagataa tattatccag
     4021 gaactgtcta acccacttgg ttggtggcta caagcacaag caaataattt tcaaggtcaa
     4081 gatgaagtat ttttgggttt agcaaagaaa attttggatt tggagtatga attagaggag
     4141 gaccccaaaa aggatgacct tgtattcgaa tcaattaatc atcccatagg gctagtaact
     4201 gaagccctgc tagattggtg gtatcgccaa gaacctaaag atgggcaagg attgacggaa
     4261 gacttaaaaa atatttttac tatgctttgc gatacatcta tcccaaaaaa tatacgtggt
     4321 cgcctaatat tgacaggtca tgctatttcc ctgttcagag ttgataaaga atggacaagt
     4381 acttatctgc tccccttgtt cgattggaca atttccaaaa ttgaagcccg tggagcttgg
     4441 aagggcttcc tgtggtcacc cagaatctat cggccactgt tatcggcaat caagagacct
     4501 ttgcttgaga catcgactca ttttactagc ctgggcgaac atgccaggca atatgcgtcc
     4561 tttttgacct ttctggccct ggattcggct aatgattttt cgaatagaga acttggcaat
     4621 gcaacaagga ccttacctaa agaaggatta gaaagcgtgg tccggacatt ggttcgagct
     4681 cttgagggtt cagatgaaca gagggaagaa tactggagta atcgaattct tccatatctt
     4741 cggaatatct ggcctaaatc tcgtgattta aatacaccca cgatatcaga aagttttgga
     4801 aggctctgta ttgccgcagg agggggtttc cccgaggcat tcaatgaact gcagaattgg
     4861 ctaaagttaa tacatggccc agatttcttg atccatctcc tctttgaagc aaagctacac
     4921 gaacattttc cagaagaaac ccttggcttt ttgaatttga tcatcagtga tgatgcacaa
     4981 tttcttccgg aggaacttat acagtgctta aatgagataa agtcatcttc agaggaatta
     5041 tccggagatg cacgcctgga ccgcctgtat agtctttacg aaaggcatgg gaaatcagga
     5101 taattacgag gcaatcttgt ttaaatatat ctcatgccga aacttgtgat agaaggtttg
     5161 cttggtgaca ggtgattttg tgtcattatg tttggaatga caaaagaccg acaaaacagc
     5221 aaaggtcgcc cttttcagga cgacctcgtt actccttcta cacatggcag acgagctaac
     5281 cagatcatag atgacgtttg acagagaaac aagtacgaaa tcacaaaccc tatacaggac
     5341 ctgatagcgc aggaggctgc ggcatgaacc gaaaatcgct tgcggatctc atagcactcg
     5401 gcgaaggctt tacaaccgaa ttcaagaagg ctggaacctc caacctgggg cgtgagatat
     5461 gcgctttcgc gaacgccacc ggtggagtga tcctgatcgg cgtaactgac cttggagata
     5521 ttgttggcgt agataatcac aaccggctca aatcggaaat acagaacata gctcactcgg
     5581 cggaacctcc tatagcagtg gaagttgaga gtacaggaaa agtcctttgc ataaatgttc
     5641 ctcagcagca cagcaagccc tactctttcg ggggcaagtt cttcatgcgc gaaggatcat
     5701 catcgcagca gatgtcccac caggagatac gggaattttt cttcaaggaa ggtttgattc
     5761 atttcgacga aactccatgc acccgttttt ccctgaagaa cgatctgacg gaagaggcct
     5821 ggtccgtgtt caggaaacga gccagaattc ctgaaggcat ggacacaatg gtagtgctgg
     5881 aaaatctgca tctcgtcagg gacggacaga tgacgcacgc gggggcctgg ctgcttgcaa
     5941 gtgatatcca gaagttcaat attagtgccg atgtggcctg tgcgctgttc atgggcgtgg
     6001 acaaggtacg cattcttgac cggcgcggtt ttaacagcga tgtctattcc atgatcgacg
     6061 aggtagtgac ctacatcctg tccaagatca acgtggagta cattatcaaa cacgtaaaac
     6121 gcgaagagcg gccggaactg ccggaggagg cgctgcgcga ggctgtggtc aacgcgctgg
     6181 cgcaccggga ttacagatcc acggccaatg tgcaggtcta tatattcaag gacaggatag
     6241 agatagtcag ccccggaggc cttcccgcag gcatgacaga ggcggatctc gggatcaaaa
     6301 gcatcccgcg aaaccccctc ctgtttgcaa tgctgcaccg catggaagct gtggagcata
     6361 taggatccgg catcaaacgc atacgtgatc tgtgtcgtga gtatggggtg gccgaacctc
     6421 gaatcgaggt gtcggagcac tggttcacga tgattttccc gcgtccggga gtggagccag
     6481 aggcagagct cggattcgat accccgcatg ataccccgca tgataccccg catgatgccc
     6541 cgcaagttgt aaaacttgcg gaagcacttg tcggagagat gaatcgtgaa gagatcatga
     6601 ttgctcttgg acttaaggat cgaatgcatt ttgctaacta ctatctcaaa cctgcgcttt
     6661 attccgatgt tatcgagatg acgattccag ataaacctag aagcaaaaac cagcgttatc
     6721 ggctttcagc caagggaaag ttgttattgg caagtcgtga gaagagtaga aaatgacccc
     6781 gataccatac accgaagaca ccctcgttca gcagaccacc gccgagtacc tggaaaaaca
     6841 actgggctgg gagtctgttt atgccttcaa caacgaggat ttcgggccag agagcttgct
     6901 tgggcgggcc acgggccgag atgtcgtgct tacgcgttac ctgagaagca gcatggagaa
     6961 actcaatcca gggctgcctc aaacagccta tgatgacgcc gtgcgtcaga tcgtgacgat
     7021 agcggctaca caatcactgg ttgctaccaa cagtgacaag tattctctgg tccgcgatgg
     7081 ggtcaaggtg acatttcgta acgaaaaggg tgagcgggtt cgccagttga taagagtttt
     7141 tgatttcaac gaaccgtcaa acaaccactt cctctgtgtg agagagcttt gggtgcgggg
     7201 ggacctttat cgtcgacgtg ccgacatagt ggggttcgtt aacgggctgc cactgctctt
     7261 catggagctt aaaaacgtca gcagggatat ccgtgccgca tacgaggaaa acctcaagga
     7321 ttatcgggac actgtgccgc atctctttca ccacaacgca ttcattgtcc tggccaacgg
     7381 tgtggatgca aaactgggtt cggtgacaag ccgcttcgag cactttcacg agtggaagcg
     7441 tctggcggag gatcagccag gtgcagttga gatggagacg ctgctcaaag gtatatgcga
     7501 caaacacaat tttatggatt tgtttgagaa cttcatcgtc tttgacgact catcgggcga
     7561 aaagaagaag attctggccc gcaaccacca gtatctcgga gtgaacagag ccgtcgacgc
     7621 agtccaaaac agaaagagcc tcaagggcag gctgggggtt ttctggcaca ctcagggagc
     7681 tggcaagagc tattccatgg tgtttttcac ccgcaagatc catcgaaaac ttggacgcaa
     7741 cttcacgttt ctgatactga ctgatcgtga tgatctggat aagcagatat atgatacctt
     7801 tgcgggttgc ggtgtcgtga acaatgatcg tgaaccgtgc cgggcatcca gcggaggtca
     7861 cctcagtcgc ctgctggccc agcaaaaatc gcatatcttc tcgcttattc agaaattcaa
     7921 tttggaagtt gatcccgatg aaggctatac gcagcgtgac gacattattg ttatcaccga
     7981 cgaggcgcac cgaacgcaat atgggacgct ggcgctgaac atgcggaacg ccctgccgaa
     8041 cgccagttat atcggtttta ccggtactcc gcttttcaaa gacgatgaga taacaaggcg
     8101 tgttttcggt ggttatattt cgacttatga cttccagagg gcggtagagg acaaggccac
     8161 cgttcctctt tattacgacg caaggggaga aaaacttggt gttgcgatag gtgacgttaa
     8221 cgagcggatc gctgaaaagc tggaggaatt tgaaagcgaa gacatcgatg tgcagcagcg
     8281 gttggaacag gagatgaaac gggactatca catcatcact gccggcaaga ggctggatca
     8341 agtggccagg gatttcgtga agcactattc cggagcctgg gagacaggca aggcgatgct
     8401 ggtgtgcatc gacaagatca cctgcgcgcg aatgtacaag ttgatcgcat tttactggga
     8461 tcaacgcata aaggagcaag aaaagagtct ccttaaggcg gagggtgaac aggaagaggc
     8521 ctatctgctc cgccagatcg aatggatgcg tcagacacgt atggcggtag ttgttagtga
     8581 ggaacaggga gaggtggaga aatttcgcaa gtggggcctg gatattacca cgcaccgacg
     8641 cctcatcaag gaaggcatgg atctgccgga agagatgcgg cgcctgcccc agtaccacac
     8701 aatccagagt ctttccctcg acgatgcatt caaggaagag gagcatccgt tccggatcgc
     8761 catcgtttgc gcaatgtggc tgacggggtt cgatgttccg tgcctctcga ccctctatct
     8821 ggacaagcct ctcaaggcgc atacgctgat gcaggccatc gcgcgggcca acagggtcaa
     8881 cgaaggcaag gaaaacggga tgatcgtgga ttattgcggc atactgaaga acttgcgaaa
     8941 ggcgctggcc acatttgccg gagccgggga tggaggccgt ggtaaagagg gcggagagat
     9001 cgaacccgcc agacctgacg gagagcttct tgacgatctt gctgaatcaa ttgcccttgt
     9061 ctgtgctttt ctatcagaat acggaacatc ccttgagggc ataatcagca attcgggttt
     9121 cgagcgcaac gcggcaattc ttgcctgcaa ggaagctgcc aacagcaatg atgagacccg
     9181 caagcgcttt gaggtgatgt gccgggctat tttctcaaag ttcaaggcgt gtatcaatgt
     9241 ccaggggatc aacacccatc gtgccaggca cgacgcgata agcatcgttt acaggagcct
     9301 ccagcaggac agggaaaaag ccgatattac cgacattatc cgtagccttc aaaaagtcat
     9361 tgacgaggcc atcgtgacga agccggaacg tgttgcgagt ggatccgcac cttatgacat
     9421 cagcaggatc gacttcgatc ggctgaagaa agaattcgct cgaagtcctg caaagaaaac
     9481 cactgtacag aatctgagac agctagttga gaatcgtctt caacgactac tggagcagaa
     9541 cccgttgcga acagattttc agcgacactt tgaagagatc attgccgaat acaacaacga
     9601 aaaggaccgg gtgacgatag agaagacatt tgaggaactg ttgcgttttt atgaagacct
     9661 gaacgaagaa gaatcaaggg cggtcagtga ggggcttgac gaggaatcac tggcggtatt
     9721 cgatctgctc aggaaaccgg atcttacagc ctccgaaaca aaccggataa aaaagatctc
     9781 ggtcgatctt ttggagaatc tcaaggccga gaaactacgc atagaccact ggcgtgacaa
     9841 agaggcgacc cgcgacgcgg ttcgcctctc gatcagtgat ttcctatgga tcgaatcgac
     9901 aggtttgcct gttgatcggt attccgaaga agacgtaaag aaaagggcag aggaagtgta
     9961 tctacatgtt ttccgcgtct atcccgtcct gccctccccg ttctaccagg cacagacggt
    10021 ggcataattc cgcttccggt tcacgcattt gatctcgcct gtcatcctga gcgaagcgaa
    10081 gcggaacccg ccagaaggcg gacgttccgc gccctttgcc cacaggcgga gatctggatc
    10141 taacccggat tcttcgtccc ggtggtcctc agaatgacag gcaagagcaa ggagactggc
    10201 atctggagac gggataccgg aggctggaag ggcaagagcc ttgttcttgc cttttccgtt
    10261 gactgttgac cgttcaccgt tgacgccttt gattttgtca tcctgaggag catagcgatc
    10321 gaaggatctg cccttgccgt tcaccgttta ccgtttacgc ctttgctcct gctttcctgt
    10381 ctcctcccgg tctcctgttt ccttgtaatt tgatcttaaa catccaggtt tctgacttcc
    10441 ttggcatggg cctctatgaa ctctctgcgg ggttctacct tgtcgcccat gaggatgctg
    10501 aagtactcct cagcggagac cgcgtcctcg atctcgaccc ttttgcatat gcgggtagca
    10561 gggtccatgg tagtctccca gagctggtca ggattcatct cgccaagacc tttgtagcgc
    10621 tggatcgtca gcttcttgcc tttggcagag tcctggatgg ccttcagttc cttctcgctg
    10681 tagcaataat taacttcctt gcccgtctgc gtcctgtaaa gcgggggttg tgctacgtag
    10741 aggtgcccct tttcgataag ttccggcatg aagcggtaga aaagggtaag aagcagtgtc
    10801 cgtatgtggg atccgtctac atcagcgtct gtattgtggc aacagatgcc gcccattccg
    10861 cagataaagt tctcgtcctt ttcaacagaa aagtcgtaga cccatcgggt ggatggttcg
    10921 acttccttga cttccaggac cttcagtcca aggatatctc cttgtaatgg aacaaaacgg
    10981 cgattcttgt ttttgcttga accgttgttg attttttccc acaatttccc ggcaagacga
    11041 tggtcttgcc agacgggttc tatctttctg agatcatcca gggccgaaac ggttatcgta
    11101 tggtacctgt gtttcgtcct taccggtttg ctccggatca tgccttctgt atctgaaggt
    11161 tccctggagt gaagggtagc gactatatcc agagatgaaa gcaggaagag cacgccggag
    11221 gccaggtctt ttgacgttgt tgtgacgcta ataccggaat ctcttatggt tccgtctccc
    11281 aggaaataac ccctcaagaa tgctttctga aggtttttat cagtgttaaa gactatgtcg
    11341 ggaacccttt ttgagaagga cctggctccg tcaaacccga aaacaagccg gaataccgtc
    11401 gaaagaaccg aattaagaat tttcagttcc ccgctgcgtc catttttgcc cggataccag
    11461 tgaagatcca ggccgaaaac acggctgaac ctgtcacaaa cttccttccg caggggttcg
    11521 ttgtttgagc caatcgcaaa ccggatgcct tgtctctggt ccaggcttcc ttctgctgca
    11581 aaaaagccga ggatggtcag gagatcatcg tcaacaggta tattcctggg cactgctctg
    11641 gaggcatagt gttctggcgt gagggctatc cccgcgtctt caaggcaggc cactccttca
    11701 ggagaaaggt cctggattct gatgtatggt ttgaccctgt tggaacagga gccccggtat
    11761 tgcttgttcc agacgttttc aaggttcgaa tcagttgtcc ggaccttgtc cataatgtta
    11821 tccggatcga gaccaacggc tttggcatag ttctggaagt tggttacggt agggcgattc
    11881 gaaccgtttt cccaaccata aaaggtaatg ggttgcctga tccctgtttt ctggcatatt
    11941 tccctgtttg tgaagccgca agtttttcgg gcgcccttga gcgtttcccg aacatcttcg
    12001 ggaatttcga cacgtctgtc agtaagttcc ggtttatcgg aatactcctc gagtatctca
    12061 ttcttaagca tgtcctcaat ggctttaccc cggacataaa ggttggaatc aagtctgtcc
    12121 ttcctggcga aaagttcctt cagaatatcg agtttccgga tgcccttccc ttctattttg
    12181 atggtgcggg gggcaacgag caggtctcct tcgacgatct cgcttccctt tttcagtact
    12241 acccggcctt tgtcaacggc aaaaacactg tgggaagagg taactctgac gtttcttccg
    12301 taggcagttc ttatttcgta aagaggttcc tcaatagtgt gccttattac ttttttcaga
    12361 ggcctgaacc ccgtattacc ggtttccaga tcgaagcaca ttacctcgta tccatcgggg
    12421 caaagagagc catctatggc cttgtctatg aattctccaa ttcggacctg gcgaacggac
    12481 ccgaagggat ccttaaccag tgtcggctcc tccccgtcca cgctcataat gaaggtttta
    12541 ttatatctga tgttatttac gttaaaatca tcgccaacac ccgctccgag tgcctggatg
    12601 atcgtgcgga tctcgttgtt gtcgagggcc ttgtcgatgc gggccttctc aacgttcagt
    12661 atctttcctc ttaacggcag tatggcctgg aagtttcggt cacgtccctg tttggcggaa
    12721 cctcctgccg agtctccctc gacaatgaat atttcgcact cctcggggtt tcggctggag
    12781 cagtcggcca gtttgccggg aaggtccagt ccggcaaggg ccgtcttgcg gcgcaccagt
    12841 tctctggcct tcttggcggc ttccctggct agtcttgccc tgactgcttt ttccacgact
    12901 gtcttcagaa tgtcgggcct ctcgtcgatg tctgccatca agccatcgta gaagagggag
    12961 tcgacgatcc ccttgatctc cccgttgccg agttttgtct tggtctgtcc ctcgaactgg
    13021 ggttctggca gtttgacgga tatgacagcc gtaagacctt ccttgaggtc atcacccgtg
    13081 aggtttgggt ccttttcctt gaggatcttg tttctgcggg ccgattcgtt tatggcccgg
    13141 gtcagggccg tgcggaagcc cgatacatgg gttccgccct ctattgtgtt aatgagattt
    13201 gcaaaggcga agaccctctc gatgtagctg tcgttgtact ggacccctat ctctacggtg
    13261 accccgtcct tttcccctga tattacgatg gggggtttga aaaggatgtt ctttccgcgg
    13321 ttcagatatt ccacgaagga ggatatgccc ccctcgtagt gaaagatctt ctccttttcg
    13381 gtgctctctt ctttcagttt tatggccaga cccggattca ggaaagccat ctccctgaac
    13441 ctgcccgaaa gggtatccca ggagaaaatt acttcctcga atatttccga gtcggggagg
    13501 aactctatga gagttccgtt cttctccgtc gattctccgc agcagaggtc gccaagagga
    13561 atgcctttgt tgtatgactg tctccactct ttaccgtctc gccagaccgt gagtttgagc
    13621 cactctgaaa gggcgtttac gacggatacg ccaacaccgt ggagtccgcc gctgacctga
    13681 taggatttgt ggtcgaactt tcctcccgca tgaagcgttg taaggactac ctctgccgcc
    13741 ggtcttcctg tctggggatg aacatctatg gggatacccc gaccgttgtc ctcgatggag
    13801 attcgtccat cggaatggat ggttatggag atatggctgc accacccggc catggcttcg
    13861 tcgatggcat tgtccactac ctcgtaaacg aggtgatgaa gccccctggg gcccgtgtcg
    13921 ccgatgtaca ttcccgggcg gcgccggacg gcctccagcc cctcgagtat ctggatatcc
    13981 ttggcggtat actggcttga agcggatgtc ataggatctt tcctccctgg cgcaggtcac
    14041 gcgctctttc tttgcctttt ttcatgaaat tccgggatct tctggcgagg gtagcagggg
    14101 tgaaccctgt ggccataaca gagccgtccc tcattaatac ggctgtctct ccaccgaatc
    14161 tgacaagggc cacgaccttt cctgtatgca cggccatgct ttcctcaaga agaagaaaca
    14221 aatgggagtc cctcccggaa ggtcattatt acaactttcc cattatatac caaaaggggg
    14281 tgtatgaatg ttccaaccgg ctcaaggcaa aagggaaaca gtttcggcag aggagaggca
    14341 gtttacgccg atcgcttcca gttccctttt ggctgcctcg ttcaggagag atatgccgtc
    14401 ggtgacggct atgacccgga aatccctgtc gcatgcctcg tatatggtgg cccttgggca
    14461 gttcggatag tttgagcccg taacggcgag ggtgtcaacc cccagtgcct caaggtgttc
    14521 catcagggga gtgccgaaga aggctccgaa tcgtggtttg tagatgatgt attcgctggg
    14581 gcctatcttc tggattccgc cggaaagcaa aagtctgtgg tcgagaagtg tcgatgggtc
    14641 tggcagcagt ggggccgcga tctggctgga gacggaaccc ggcactaata gaggttctcc
    14701 tgacaggatg cgcgacctgc ggcaaagatc cgcattgctt gcgtcgggca ggtacaacct
    14761 gacgatgtgg atcaatggtt ttgccgctct ccggaaagct ttcgccaggt gagccgcggc
    14821 ggggaccgtt cttgcgttct cttctctgcc cgatgaacct cctggttgaa actctctctg
    14881 gatgtctatg gtaagcattg ccatccttga cgggtcaggt cttgtccatt cgtccatctg
    14941 tttccctccg caattaattt tatcatgcgg aaaagtcggg gcatcctgcc tgtcaggaaa
    15001 gagctgaaaa aaatagacca accctgactt taatggtttt acgatccgct attcacggta
    15061 ctgatttcat ccagaacttt ctgcgcgtgc ccggccgccc tgacgttgtc ccaggtcctt
    15121 gcgatctctc cttcggggga tatcaggacg gtgcttcgga ctacccccat gttctctttg
    15181 ccgtacattt ttttcattct ccaggctccg tacccggtca ggactttctt ttccgggtcg
    15241 ctaagaagga taaccgtgag gttgtgtttt cctatgaaac gggcgtggct ctcgcacgtg
    15301 tcggggctta ccccgataac aacggccttg tttcggctga attcatcctc cagggccgaa
    15361 aattccatgg cttcaagggt gcatccgctt gtgttgtctt tcgggtaaaa gtagagaact
    15421 acccatttgc ccctgaattc ctggaggcat ttttcatcgc cgttgctgtc cctgaggcag
    15481 aaatccggtg ccatgcttcc gatttctttc aaagtctgat cctcctgtcc cgttatctgg
    15541 ctggcagatg tttttccggg tcctggtgct tgccgttgat caccacctcg aagtgcagat
    15601 gggggccggt agtccggccc gtccttccga cggtagcgat cttctgtcct gccgctacag
    15661 tgtccccggc tttgcagagc attgtgtcgc agtgggcgta tcgagtcatc actccgttat
    15721 tgtggtcgat gagaatggac cttccgaaac cgctcctcca ggcggccctg acgacggttc
    15781 cggctgctgc cgcgtatatg ggggttcccc gcggggcgga tatgtctatt ccagcgtgga
    15841 atcgccctct ccttggacca tatttcgaat atatcttgcc ttctactggc catgaaaggg
    15901 agaaggggcg aacctccgct gtcctgacgg tgtttttgtc gggagaagct tcggccgtgc
    15961 ccttgacggg ttccggttcg gggagtgtct cctcgctgtg atagaggttg gctgtcgttt
    16021 cgccgttgcg cctggctctg acttccgaca tggtctcaat cagaaggttc tcttcccgtg
    16081 gcacgagaat ggttgtcagt tcaaaaggga tctcctctcc agggagttcg ttggcccacc
    16141 tgatgaagta gatctccatg ccgtatttgt tcgatatgga tgccagggtg tctccctgcc
    16201 ttacctcatg ttctatccag ggaagatctt cggcgaagag ggtctctcgg ggcatggaca
    16261 gcagcatcat gggaagcagg aggaaaagaa aaagccggat actcttcatc ggaggaccac
    16321 cctttcggca ttgggcctga attatggttc tgtcacttcc aatattattc ctgtcccttt
    16381 taccgtgaaa agggtatttc cacctattga tccttatggt ttcagatgga ttccggaaca
    16441 gttcctgcgc cacgggcagg atgggcacat tgcaaggttt ggcgaaaaag ggcccgatcc
    16501 cgcatttttt gccgtggaga taatattatc ctccatgtcc ggccatgaga catccctggt
    16561 gtcgatcgtg gtctcgcccg ggtcttctat ggaaaccaga gcaagatcta tgggggcatc
    16621 gccgaagtgt ttccgtgcgg caagggcgta gaagagcagt tggctgtcat aaagtgtctc
    16681 cgcgctgccg ctgatcatcc ccgttttata atccctgatg aagacgcggc cttcactctt
    16741 ccagactacg tcgatggcac cgaccatcag tgtcccgtcc ttcagacgaa cacggaaggg
    16801 cgcttctttc attgcgtctt tggcacaacg gatcctttcg gccgcgggag aggccgagaa
    16861 cttctccagt tgcgcgatca gcgggatgct tctggactgg ttggcccata ccggcctcag
    16921 ttccggcggg aggacgctgc caagggagag cgagacgcga gggtcgaacc ttgggagggt
    16981 ctctgggcgc aggtcccatc gtttgagtat ccagtgtgcc aggctcccca gatcggatcc
    17041 accgtcccct ccggagtgac tctcccagga gatgtccatc ccctgcctgt gtctcatgcg
    17101 aaatgcgtat gggcagaacc tgaagagagc gtaagcagtg gcacttatcc tttccagcgg
    17161 tttttctggg agttctggag ctggaacaga acctccctgc tccgcctccg gagtttctct
    17221 tatccctgtc ttcccgggtt gtacctcgtc aggccttaca aggggaagtt tttcgatccc
    17281 gcccagccac gatgagattg tcctgagcca ggagtgatcc ttcggtttgg ggaccccctt
    17341 gcttacgggg cagattccgc agagtatcag cgaatctctg gccctcgtgc aggcaacgta
    17401 gagaagacgc tcgttttcct ccgtttcctc cctcgtttcg aagagatcgt ggatggctcc
    17461 gccgaatata tcgtttttgc attcgtagtc ccactcttcc ggtattcccg ttgccgctac
    17521 acctagtttc gccgacggaa tgatccttgt tccctgcccc cgggtcctga tccctctttc
    17581 taggcctgta actgcgacta ccgggaattc cagccctttg gcggaatgaa cggtcatcac
    17641 ccttaccatg tcatcgtctt cgccgaggat gtccgcttct ttcgagtcga tgcctctgga
    17701 agccacatcc ccgaggtagg ccgcgcagcc ggagaggctt cttcccatgg ctccctcgta
    17761 ttcccttgca agatccacgg ccctccgaag gtttgccgcg acacgggccc ttctccatga
    17821 aggataggag gcgagcacac ggctctcgtt caacagcaag gccagggcga ccgaggggcc
    17881 ggaggccagg cccgcttttc tgcaattatc aaaccactcg gcagcatcgg gggatttttc
    17941 ggaaatccag tcgatcagcg gcttattggt gccgatagat ccggagacca gttccgatgc
    18001 atgttcggga gagaggccgc tcaggggcga ggacaaaaat gaggcaagcg caaggctgtc
    18061 ggagggatcg gccatggctt gcagcaggct gacggcgtct tttacctcac ccctctcgaa
    18121 gtagttcctg tttctctcaa aataggtagg gatcccgcgc tcttcgatga agatcttctc
    18181 tatgatcccg tagtaacctc ttgacgggac cagaacggcg atgtcgcggt aagcggcggg
    18241 tccgggagag aatgttccag ccccgtcttc acgccatacc gtagcccccg tcgaaacagt
    18301 ttcgactatt ttgtcggcca ggacacgcag ggccgcctct cgaagctggg gtgtccttgc
    18361 cggtttttcg gatccagggt ctgaagcaat gatgatttcg aaggggcttg ccgagatctc
    18421 ttgccgctgt ttccaccact gtcgggactc cggaggcatc aggggggaga attttttgcc
    18481 tgtggaactg gcgatgccgt ccttccatgt ttcgccgaaa agaccgttga tactgtccat
    18541 aacgtcttgc ctgcagcgga aagtaacatc gaggtctatc agcctgcacc cggacccgcc
    18601 cggggaattg atccgtttcc agaaaatttc gggctgagcg tggcgaaagc ggtagatcga
    18661 ctgctggagg tcccctacca ggaaaagaga gcccccctcc gatggcgaca gggaggtgat
    18721 cagttcatcc tgcagcgagt ttgtgtcctg gaactcgtca acgatgacat ggcggaattt
    18781 cccggcaaag gatggttcgc caacgatggc tttgcgggcc ccccggatca tgtcttcgaa
    18841 ggatattgtc ccccgggaaa gttttgtcga ctcgaagtat ttccagaaaa gtgatatcag
    18901 gcggaggagt ttttcccgaa ggagtgtctc ctccggggag gagccccagg ttatccattt
    18961 tgcggcgctt acccatgaca agcgggaatc cctgtattct gcaagaggct cccctgttat
    19021 tttcagaaca gatcctttga cctgtttcat gcacttgccg gtgcttccct tgagattccc
    19081 gataaggcct ttctctttat ccataagagc aagagcaaag gcgggaagtt tttctttgcc
    19141 cggaggggaa gtgttccatc ttttgaagaa gtccagggcc ctgccggcaa aaatcgcctt
    19201 gtcacccttg aaaatttcag gtgccccggc gtcgggcagg atcccttcta tccagacctt
    19261 ccaggcctct gtccacaaag ggaccaggga atccagaagt tcacgggaaa gcgctttgtc
    19321 gcgttcgacg atattatccg cccagaccca gagatcttcc ggagagatat ttcggctctc
    19381 gaaaagagga atggccgaag acgcggccct ggacaggttg attgctccga aggtgttgat
    19441 tatatctgcc gttgtgttct cctccagaag gtttgatcct ctttctcgcc atatcgggtc
    19501 gagagattct gttagagggt caagatcttc cctgtcgatg gctctctcga ggaactgcca
    19561 gaaggagttc tcctccggag gtgatatcac ccttatgttt ggatccatgt cgacggacag
    19621 gccgcactct ctcagcactc gcatggaaaa agaatgaatg gtggagacgt aggcctcgtc
    19681 gagacgggaa agggcttcct ccatggctcc gcagaattcc ggcatgtcag aggacagcag
    19741 tgccataagg cggcgtattc tttcccgcat ttccatggcc gctttttcag taaaagttat
    19801 ggtcagtatc ctgtcgaccg gaatccggtc aacggcgacc agtctcacga atctccacgc
    19861 cagggtgaga gtcttgcctg taccggcccc cgcgctgacc acgatcatgg ggctgtcgga
    19921 cataacagct tcaacctgcc ctggttccag ggcggcttta ttctccaggt attccctgaa
    19981 tacttcagtc atcgttgccg tcatcttcgc cgctccccct gcccgtcgat tcgcttttcc
    20041 tgcaaagccc gtgaaaatcg cagaacatgc aggcctgggt gttctcgtag ttggggatga
    20101 acctgctttc ggaaatggag agagccatat cttccatgac ttttttggca gaccctatca
    20161 tatcctcgag tttcgtctga ttttttttga tccatttgcc cagtatcccg gccgcgtttc
    20221 cttcaaggat ggctgtaact ccgttatctc tctgggacag aaagatgtat cccgaaattt
    20281 cgatggatcc ggtctccctg agagcggccg cataggcggc gagctgtaga gagcgggaca
    20341 gcccgttgcc gcggcctgtt ttgtagtcga agaggagagc ggtaccgtcc tgcaggatgt
    20401 cgagccggtc gtaccttccg gagaaaggaa tgccatccac ggtgatgctg aacttttctt
    20461 cccgtttctg gcctgtcctt gcaagggcga gtccggaatt ttccatgtcc tgctgcagat
    20521 gagccatttt cagggtttcc ttgcggagcc tgccgaggtg gcgggggagc ttttcaaggt
    20581 ccggatattg ttcatggact gtctcttccc agtacctttc cgcaaggcag aaaagatctg
    20641 ccccggaaga gagcctttcc gtccagacct tctcccagag ggcgtggatc atggttccgg
    20701 cggccagtgg atcaaacccg caggcccagg tttttcccag gccgaggacc cgccctgcgt
    20761 agaatttgaa ggggcattcc agccaggtgt cgatgctgct catggaagcc ctctgagcca
    20821 cccccacggg ccacggagtg ggcggaggaa gggccgaaag acgtccgggc attcgcgggt
    20881 ggtcgttctc ccgtatctcg atattggaga tcactgtttc gcctcctgac ggaaggacgt
    20941 ctttcatgga tctggcgaaa gggggatcac ctcctgtggg tgtgatccat gggtcgtcgc
    21001 cggatattgc tgcctcgagc atcatcgaag ggagaagggg cctgcctgat ccatcctgcc
    21061 tgggccggga aaggatgcag agatcatcag ccgaagcgat gatccttctg aggagtgcct
    21121 cccgctgctt gcgtttctcc cggagcaggg gcagatggac cggtgaaagg cctatgccgg
    21181 gatcccggtg gagggcctcc ttgcgttggt cgtcgagcaa cggggattcc cgaagtcttc
    21241 ccggccactc gttcgcggta agacccgtca caatgaagac cggatgccgg gccagcacgg
    21301 gagggcttcc cgcatagagg ctcaggcttt cctggctttt tggtgcctgc cacacagtgg
    21361 atctatcggc ccaggtcgag agaaatgtcg aggcctcggc cccgcgaagg gtcaccgaac
    21421 cagccgggcc aatatccttc tccatgtcgg aaacgctttc cagtttcccc acaagttccc
    21481 ttgatgctgc gttgagcctt ctggcgcttt cgtcaaggcc ggggtcggac attacgaaac
    21541 gggaaagagc agctccccag atggatactt ctccgttgcc ggcgacggaa cgcagtgagc
    21601 gaagaagttc cgaaggagac ccccctgact ggacgagtgc gcagaaatcg gcgattcttt
    21661 cgaaattgtc ggatagtgct ttatcaccgt gcactgcgag gaaggctttc cattcaattt
    21721 cgccccgggg tccctggctg agggctttca acagagggaa tccatcgggg gatatccatg
    21781 gctgagagag gatatgagag gtctcttccg tagggaatcc cagccgttta gcctcgaggg
    21841 cggccgcggc cgtttgccac aggggcgtct gcgaaacttt aggtccaccg cttgccacat
    21901 aggggacgcg gtagcggtta agtatctcct cggtgatttc cagcctctca ggatcgacag
    21961 agaggccaat gccggtccag cccgggaaag gaatgtccac ccttctggcg aactcgcctt
    22021 tgcctgacac ccaaagcgaa agattgcgaa cgacggtctc cagttcaagg cggaaatccc
    22081 cggccgaaag cagcagtgcc tttatggggc tgagtgctgg ccgggctccg ggagccgaca
    22141 tttttccgaa ctgctcgcgt gctgtatgga tatccagacc tgtttcgggc atgaagattg
    22201 taaccgatgc accttgattg gaaatttctc ttatgagtcc tgactgggag cgggtaaaac
    22261 tcataaatcc cgcaaagatg aaatgatgct gccggatcca tttcgatgct ttttcgaaat
    22321 ggctggacag ggcgattcgt gtaattgtcg ctgtctgggc actgtcggcc agggagtggg
    22381 attccatgta tgaaagataa tcatggtaaa gacggcagag aaggccgtgg ggtgtagaca
    22441 atcgggggca cttgccggtc ggatcgcatg aggggcatcc aagtgatgct cccagagtgt
    22501 ccggaagcac ttcctctctc aggagttcgc gaaggttctc tcccaaagtc cagataaagc
    22561 cccgcttcag agctccggga ggaaggtcgg cgcaatcgtt ttcggctctt aaattttcga
    22621 ggacatgacg aactataagc cagtgatcgg gagggtctat ctgtcggcgt ctctcttccg
    22681 gagggaggtc cagttcacgc atgacctccc tgtaaaggtc gtcccacctg aggatggccg
    22741 gctccccggc accgaaagag acactttcca gaatgatatc cctgaggatc tccctgtccc
    22801 cggacgaagg aacaaggaaa aaagggacaa gtccgctttt acttcccgag gaagaaagat
    22861 cctcttcgat atctctggcg cggtgatacc cgattatcct gatcggcatt tgctcccctc
    22921 ccgcctaata ttagcaggag gaacaagaaa ccgggaaaag agacagaggg gcaagccacg
    22981 tgagacgtga gacgtgagac gtgagacaaa aaaccaaagg cgtaaacggt aaacggtaaa
    23041 cggtaaacgg aaaacaaagg cggcaggaaa caggagactg gaaactggaa gggccgggac
    23101 cggagttaag gctttgccga aggctcagga gccggtgagt ttcgaagccg tcgcggagtg
    23161 gcaggaaaag cacacctgcc gggggccttt cccccggcac tgtgcggtcg ccccgaagca
    23221 ggcggagaaa cgaacgcttt caggcgagtt gcctctcggg aaaggcttca ctccgggccc
    23281 cggtcggtat acggatcccc ctttcgattc acaattgacg atttacgaat cacgaaaaca
    23341 aaccagattc ctcgtcattt cattcctcag aatgacaaac aataatcagg cgactggaga
    23401 caggacacgg gaggcaggag aacctgaaaa aaccaattca
//
LOCUS       DAOTIQ010000026        23417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2557_length_23417_cov_6.158120, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000026 DAOTIQ010000000
VERSION     DAOTIQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 23417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23417
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2557_length_23417_cov_6.158120"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="P5541_04855"
     CDS             <1..658
                     /locus_tag="P5541_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRV71342.1"
                     /translation="EEGADELVFLDISASLEERKTRISWVQNVADRLFIPFTVGGGIG
                     SKEEARDLIAMGADKISLNTAAVSDPSLIGRCASLLGSQAVVLAIDASRSPRGGWEVY
                     VRGGSAPTGIDLVSWAQKGESMGCGEILLTSMDSDGTRGGYDLECIEAVSSSVSVPVI
                     ASGGGGIPEHFAEAIWSGADAVLAASVFHYGSIRIPELKKYLAGRSIPVRTTGEENLV
                     "
     gene            655..1287
                     /gene="hisIE"
                     /locus_tag="P5541_04860"
     CDS             655..1287
                     /gene="hisIE"
                     /locus_tag="P5541_04860"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRV71343.1"
                     /translation="MMQYADQIRFDEKGLVPIIAQDADTGEVLMLAWGNRESLVETER
                     LKEMVFFSRSRNRLWRKGETSGNTLAVVEIRRDCDNDALLALVRPSGPVCHTGSFSCF
                     SSHGEKFAGFSCFAGKLWRYLLSRSKASPEESYTARLLSQGLSRTGQKVGEEGVETAL
                     AVATGNTEAFILEAADLVYHLMVCCLALGVSPPMIMEELEKRHVEKKTDD"
     gene            1280..2104
                     /locus_tag="P5541_04865"
     CDS             1280..2104
                     /locus_tag="P5541_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV71344.1"
                     /translation="MTDTGLVFDVDGVLVDTSLSFPAVVSAAVQIGAKDILGHPAPFR
                     LFDDEHYNIARRSPMFNDDYDIAWVFLSWIASGSALQREEGPVSRLDWERLIASCDGS
                     PEEWCGSVFQSPVDRGSVRKTCEEIYFGEEQYLLFRGVSPEIRIGGLWKRERPCLEKE
                     WESLDIPTGIYTGRNRQELQLALNLLGWDGFPPHLCITPDEGISKPSPRGLKILEERL
                     SVSRIIFFGDSESDRKAFEAYGKGLFVPIGPSFDSERGGFPDIRTALEFPWISKPQ"
     gene            complement(2098..3159)
                     /gene="citC"
                     /locus_tag="P5541_04870"
     CDS             complement(2098..3159)
                     /gene="citC"
                     /locus_tag="P5541_04870"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HRV71345.1"
                     /translation="MFGIEFRLTEKPSVAELAGIEKFLGRFGLSMEGVPDVSVFAEGP
                     SGELVGTGSLKGNVIKMMAVDPDWQEAGLSGSIISRLVERGRASGLTHFFVFTKPDSA
                     DKFHGFGFREIARYEPFATLLEMGQPGIDQFRDYLRGSRVNMGAKSRAGAVVVNCNPF
                     TKGHQYLVETGASRCDHLYVVVVETDLSSFPFRDRFALVREGTSHLENVTVIRSGEYA
                     VSPATFPSYFLKGSSVKKIASIQARLDVTLFANLFVPALGISVRFVGTEPYCPVTGSY
                     NEAMKEVLPPRGVELEEISRVTVGDGQVVSASIVREMIKADRWDVVREMVPESTLKYL
                     ESSDGKAVAELIKQSEGRH"
     gene            complement(3181..4656)
                     /locus_tag="P5541_04875"
     CDS             complement(3181..4656)
                     /locus_tag="P5541_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV71346.1"
                     /translation="MKVRLEETIISKCKKSNGERAVWWRGAWWSRKVLADLIMQYEEK
                     LRESGFGEGDRIAALMPNCPAFLALMVAVWKAGGTLTPLNLQAGMSATMDVLRHSDVS
                     AAFVPQGMDQLAEGISASGIPAMAVPLDGALPSFKSRKSEPSDPDIAVLFYTSGTTGL
                     PKGVPISHSNLHDNVSRSIEHFKDLADDDIFVNVLPNFHTLGFTTSGLLPLLGGFPQA
                     LVPTFMPPETAIESIKAADVSVMIAVPTMVALMLGAIARGTEAPTSLRLLATGGDRFP
                     EKLDGRTQQLLGVSVLEGYGLTETSPVVSLNEGLKDRKLGTVGRVLKGYEVEIRDMDG
                     NVLPVGKEGTLWLKGPSVFNGYFRAPEITAQKFKDGWFNTGDVVRMDEDGYLTIVDRL
                     TDIAIVGGFNVYPTEVEAVIQQLPGVRESAVVGVKHPVSGEIVKAYVIPTGEPAITSR
                     DVISFCRDKLAHYKVPRSVEFIEEMPRSSIGKILKRELRKR"
     gene            complement(4757..5953)
                     /locus_tag="P5541_04880"
     CDS             complement(4757..5953)
                     /locus_tag="P5541_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV71347.1"
                     /translation="MTVNADGFNLAEDLKSRAGEISHEIIAWRREFHQFPELAFEETV
                     TSSRVVDALSVMDGMEVIRGFGSPTSVLGVLGKDTGLPAVVLRADMDALAIEEESGLA
                     FSSCFPGVMHACGHDAHIASLLGAARLLSERASSLRRPVIFLFQPAEEGKAGAKKIVD
                     AGFFDKFQVKCVLGLHFWPSIPFGQMATRKGCMTALSDRIHIMINGSTSHAASPHQGV
                     DPTVIAAHILLSVQDIISREKDPQTSAVISFGRIEAGEAYNIIPGSAHMWGTLRAHEP
                     EVRDFIQGRLEEMIPLIARAHRATASVEYNRNYPCVVNDPEITSLVLEKSRTFFSENV
                     STVTEMTGQLLAGEDFAFYSLKAPSCFMLMGTGGEFGLHNPRYDVPEELIPLASAWEA
                     FLALSC"
     gene            6082..7386
                     /gene="asnS"
                     /locus_tag="P5541_04885"
     CDS             6082..7386
                     /gene="asnS"
                     /locus_tag="P5541_04885"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRV71348.1"
                     /translation="MSVPWVYISDLAKHEEKEVILKCWMYNKRSSGKIHFLQLRDGTG
                     FIQGVMVNGEVPEEQFSAARNLWLEASLEVTGKVRRDERAPSGFELTVSDLRIIHNPT
                     EEYPIGKKDHGIDFLLDNRHLWLRSANQLAVMKIRERIIWSAREFLHNGGFMLVDSPI
                     LTGAIGEGASGLFEVPYFDMGNAYLAQTGQLYAEAGAAAFGKVYTFGPTFRAEKSKTR
                     RHLTEFWMIEPEVAFYDHNDNMKLQEDLVSYIVGQVLKHCGSELATLGRDIAPLERVV
                     PPFHHITYDEAVILLQKLGSSITYGTDFGGEDETILTQQFDRPLFVECYPKKVKAFYM
                     KQHPEREDLVLCDDLLAPEGYGEIIGGSQREDSLELLHARIREENLPEESYSWYLDLR
                     RYGTFVHSGFGMGIERVVSWVCGLPHIRETIPFPRTIYRLNP"
     gene            7402..9105
                     /locus_tag="P5541_04890"
     CDS             7402..9105
                     /locus_tag="P5541_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRV71349.1"
                     /translation="MASSKWIPYKDFDGKPEYESRNWLIGSGKIGGKAKGLAFAFEHI
                     QGSELAEEVLLPSTTFVVTTEPFHDFINDNKMDWIHDEPDLEKIILAFSQGHMRESFV
                     KDLDRILGTLHDTPLAIRSSSLLEDSHLLSFAGKYFTTFAANVFDMEWRRRELSDGIK
                     TVWASLYNQAARAYRAKHGFTDRDEAMAVLVQPLIGHEHDGLFYPELAGTIFSKVYRR
                     PSPRINKEQGVTRFCFGLGTKTVDRSSARVIYLSHPNMRPEGNLAREVARYSQNSFDY
                     VDLEFGAFVSGDLKSLLPFVMQNHKFATSFVELYGDNSMYWAGSSPMIPTTPFFTFSE
                     LPKRCPHFLETAKKISAHYETEMGIPIDMEFTYETLEKKMTLVQLRPLSSYEEMASVR
                     IPKVPEEKILLKGSRMVSNGQIERTDHIVYVDPFSYGSRDNFFKIARALGKVNDSLKG
                     TRYILAGPGRWGSVNPDLGVPVDYAELCNCGCLVELGIVEKHFTPELSYGTHFFLDLD
                     VDNILYLPVFAGDDADIFNREWFENRPYETGQEEEIRIYKGDFSVFLDGDTEEGIVIE
                     N"
     gene            complement(9158..10405)
                     /locus_tag="P5541_04895"
     CDS             complement(9158..10405)
                     /locus_tag="P5541_04895"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRV71350.1"
                     /translation="MAKLNPHEMFVQQLEEAAPHISAEPEYLEILKQPKEVLEITIPL
                     RLDDGSVKVLKAWRSHHNNALGPYKGGVRFHPHVNRDEVAALSSWMTIKCATADLPYG
                     GGKGGVSVNAKELTMTELERLSRGYALGISRFVGTDIDIPAPDVYTNPQVMSWFVDTH
                     EKIHGRSEPSVYTGKPVAVGGSLGRGDATSRGGMYVLRETLRELGLLGKPLTAAVQGY
                     GNVGSYAHKLGDLVGLNFVAACDSRGGAYSAKGISYEEVSKLKRETGSIKGMKGTDGV
                     TNEELLELEVDVLVPAALEGVINAENAKNIKAKVVLELANGPVTPEAAAILEDKGILV
                     VPDVLANAGGVTVSCFEWIQGRTGDFWTEEEVHQKLDKKLTKAFQEIWKIKKEKNITF
                     RQAAYVRAVDRITLAMRFRGIWP"
     gene            complement(10603..11874)
                     /locus_tag="P5541_04900"
     CDS             complement(10603..11874)
                     /locus_tag="P5541_04900"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRV71351.1"
                     /translation="MGKRTSENVLLDIALRNFYGAAEELGLDEGLIEILGNSERKLSV
                     SIPVVMDDGTTKVFNGYRVQHSTAVGPAKGGIRFHPDVCQDECEALAMLMTWKCSLAG
                     IPYGGGKGGVECNPLEMSMGEKERVARTLAARIEPIVGAWTDVPAPDVNTGGQEMVWF
                     MDTISKMRGRLEPAIFTGKPISLWGSKGRTEATGYGVATCAIELLNAVGKEVKGTTVM
                     IQGFGNVGTYAALTLVDAGAKVVGISDITGAYHCKDGIDIHKAFKHINEHPKKLLEGY
                     TQPGLEKIPAEDLLFIDADLFIPAALEGVVNKNNADKIKVKYIVEAANGPITPEADAI
                     LDKKGILIVPDFLANSGGVIGSYFEWAQDLGGYFWTREEYNTRLIHIMKENFHRVWDF
                     TNEKKVKMRRAAFMVAIMRVADAVRMRGVFL"
     gene            complement(12172..12807)
                     /locus_tag="P5541_04905"
     CDS             complement(12172..12807)
                     /locus_tag="P5541_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRV71352.1"
                     /translation="MYFILWVIAIILVGNLLWRLASKRFSLPCPVWLHRMVEMDNPFT
                     RTNRASEIIMLLDIQPGMSVLDAGCGPGRLTLPLARAVGESGRVAALDIQEGMLERVK
                     KKLIVEGLENVDLIKGGLGDGILPSDSFDRALLVTVLGEIPEKRSAVDEIFRALKPGG
                     ILSVTEVIFDPHFLRASTVEKIAEEAGFIKGSSFGNKLAYTYHFLRSGKIS"
     gene            12959..13516
                     /locus_tag="P5541_04910"
     CDS             12959..13516
                     /locus_tag="P5541_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRV71353.1"
                     /translation="MDNLINGYRQFLNNADRGLYRDLCNGQHPHTLVIGCSDSRIIPE
                     EIFSVGPGELFVLRNIGNLCCTEEPSVASAIDYAINHLHVKRIVILSHGDCGAVKASL
                     HPEHLEENSIKRWLGAESCNGSSLEEAIKSWGIRQLQRLDSFPSVADAVKSGKLETIL
                     LYFDLASLRIDRYNGTGWMAFDQTS"
     gene            complement(14146..14268)
                     /locus_tag="P5541_04915"
     CDS             complement(14146..14268)
                     /locus_tag="P5541_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71354.1"
                     /translation="MPEEMQFLDNVVRVDQTGFLWAANIEVMSATSMKRGGCGG"
     gene            complement(14504..15463)
                     /locus_tag="P5541_04920"
     CDS             complement(14504..15463)
                     /locus_tag="P5541_04920"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRV71355.1"
                     /translation="MSQSFKVSKHDDGRRIDRIIRKIWPDLPLGAVMRAFRKGLVRLD
                     GKKVECSLHAAEGQLVDVPWDDEIALRQPLSSARKGPGPSLIEIIYADRDICLVNKPS
                     NLLSQPDTKGGESVISRVTSTIGWNDPSFSPVPAHRLDRNTSGAMVLALTGKGLRSLH
                     EAWRAGRVKKTYLALVAGDAPDEGEINTPILKTGEGNIATTDGKEGLPALTKFRKMDG
                     DGTMSLLLVELHTGRPHQARVHLASKGFPVIGDIKYGDKKKNDEWRKNGVGRPMLHSR
                     SIEFHEMDEPLGHISGKKFLAVPPEDFMNVLARKGWRLYDRRV"
     gene            complement(15466..16500)
                     /locus_tag="P5541_04925"
     CDS             complement(15466..16500)
                     /locus_tag="P5541_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71356.1"
                     /translation="MTEIQTLTTSQLKKLNPGERFRMIGVIQQIKGRTDKNNRPFWEV
                     SLIDSEGSIDAKVWSDGKWWDTVTGEKIPLEPASSDIPGLFIGSVAGAVGTVSEFRGK
                     QQYQFVELYLLDPKKYPVSGFIQSSPMELEKMEKELHEIIGSCGTVVRTFLEKVLSGD
                     LWESYRDLPAAVSHHHAYVHGLLEHTLSVTKGALALGQTYRDSGVDMDLDIVAAGGLL
                     HDLGKIESYRLNPFPSMTVPGTVIDHIALGYSLFSRLAGEFGLDKETADAIGHILVSH
                     HGRKEYGSPVLPATPEAMVVSSADELDFIMFCWKTFPESGNGEPSISDFHPSAGRRFW
                     RRKPAEEGGV"
     gene            complement(16569..17780)
                     /locus_tag="P5541_04930"
     CDS             complement(16569..17780)
                     /locus_tag="P5541_04930"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRV71357.1"
                     /translation="MKGRLEMLRELAVRSDTKMVLLVMDGLGGLPSVEGLTELESAFT
                     PNLDMAALRGETGLLEIVDTGITPGSGPGHLSLFGYDPRDHFIGRGILEAMGTGAEVL
                     PGEICARGNYSTWGEGDLILDRRAGRIDTESSSSITSLLGRSIREIDGVSVRFYPGLE
                     HRFSVVLSGEELGEKVTDADPQIEGKPMVWAEPLDDSGRKTATVINSLIRKVREVLSG
                     NEKANGCLLRGISGTPDIPLIPDLYGIRAAALATYPMYRGLARIVGMDVVDAGKTVES
                     LFQSLRKNWDSFDFFFVHVKYTDSRGEDGDFEAKKEVIEKVDTLLPDLLSLDPDVLAV
                     TGDHSTPAMMGGHSWHPSPLLVCSRFVRPDTTSQFGERSCSLGSLGLMPGSKLMGLML
                     AHAGRLAKYGA"
     gene            17962..18612
                     /locus_tag="P5541_04935"
     CDS             17962..18612
                     /locus_tag="P5541_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRV71358.1"
                     /translation="MKRIILRISLIALVVSLAASPVFAALSPESVTPIWANLTRTAGL
                     TDAGPVNIENKKEPNAWVSFSANKYSVHATTGLLETLKDEHELAGVLAHEIGHIKLGH
                     YNESINRSLLWLLLYKALGEKKVGGVDVLGAGIVLAEAGFSREQEIEADDYGVHLAAK
                     AGYDPWGLYNAFNSMKKAGYKTSPSGFNSHPPTERRMIHVKATTEAVEKQYPRKNP"
     gene            18708..19916
                     /locus_tag="P5541_04940"
     CDS             18708..19916
                     /locus_tag="P5541_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV71359.1"
                     /translation="MSDFFDPLFSRSAKNIKPSPIREILHLVRKPGMISFAGGMPDPE
                     IFPIEQFQEAANIITREGKDIMQYGTTEGYPPLKDFISEWTAPKMGRKLQHEEILITA
                     GSSQVVDLLCWALIDEGDYVVCEDPTFLGATGTMRNHKARFLTVPCDENGMQVDLLPD
                     LIEKTLAEGGRIKFIYTIVNFHNPLGCDLSLERRKKLLDIAKRYGILILEDDPYGYVR
                     FDGEDIPSIFSMDRDGIVLFACSFSKILAPGTRVAWCAGDKDLIRKMTVFKQGTDVCT
                     SVVVQAMVWEYCKLGHLDSFLPRIIDHYRNKRDRMEAAFHAHLPLDLISWQKPAGGFF
                     YWMTTPGIRADRLLEKAVDKGVAFVLGSAFYPTDSGGDNNFRMCFTFASPEMTDTGVK
                     RLGEALRELA"
     gene            19963..21174
                     /locus_tag="P5541_04945"
     CDS             19963..21174
                     /locus_tag="P5541_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV71360.1"
                     /translation="MSGVWESNFSNKAMNVRPSPIREILSVIKQPGMISFAGGMPAPD
                     VFPVEEFHLGAEALLKDGKNLLQYGTTEGLPELKEFLSEWTAPRMGRKTALDEMLLTT
                     GSQQVLDLMGWAMIDPGDYVITEDPSYLAALTAFHNHGARFIGIPTDHEGMMVDMLPD
                     KIEKARREGKNIKFIYTIVNFHNPGGCTMTIERRKKLVEMSHKYNIPIFEDDPYGYVR
                     FDGEHLPSIFSMDPDGGVIYAGSFSKILAPGTRIGWATGNKDIIRKLTVFKQAVDLCS
                     SPITQALVYEYCKMGYLDKHLPKIISNYRTKRDAMEASFRKHLPLEEISWVKPEGGFF
                     FWLDMPNIDAKTLFDKAIEKKVAFVTGTPFFSNGGGEHNARMNYTFCQPDIIDEGVKR
                     LGEAAREILEG"
     gene            21184..22548
                     /gene="mnmE"
                     /locus_tag="P5541_04950"
     CDS             21184..22548
                     /gene="mnmE"
                     /locus_tag="P5541_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRV71361.1"
                     /translation="MNGLIAAISTAWGESGISVVRVSGKGSRDLVDGFFRGGVSLGET
                     PPRFMRNGFITDNDGAEIDEVLAVWFREPKSYTGEEMAEIQCHGGTVAARRCLERCIS
                     LGARMALPGEFTRRAFMNGRIDLAQAESVLGIIRARSDRSLRSAAKCLQGSLSARVKK
                     IDEELLELSALGEASLDYPEEDIPTLEMDFFIKGLSKTGEKIRTLLSSARSGRFLREG
                     VRVAIAGRPNVGKSSLMNAFLQESRSIVTSLPGTTRDIIEEVITHKGIPLRLVDTAGI
                     RLPKDPAEQEGVERAREAVQKSDIRVWVIDGSEPLSDDDLALSTSLQGTCHVVALNKS
                     DLPQAVNVTDIIKILPGADVRVISTVSGEGIESLKDLIVLSSAGITSMDEDLNTTERQ
                     ITEMSMALDLVINTMDSLTAGAPVDTALETLSEARSCLGRIIGVDADESLMEKVFSSF
                     CIGK"
     gene            22551..22766
                     /locus_tag="P5541_04955"
     CDS             22551..22766
                     /locus_tag="P5541_04955"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HRV71362.1"
                     /translation="MYQRRYHKMPIIQVHLLEGRSGEQKKKLVSEMTGAVCSSLDVVP
                     EQVRIILSEMSPEDYSVGGTLFSDKKK"
     gene            complement(22787..>23417)
                     /locus_tag="P5541_04960"
     CDS             complement(22787..>23417)
                     /locus_tag="P5541_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV71363.1"
                     /translation="YLVVQNGPEQVVEWFKETRGMELLERQREEGKGAVLLMSHFGNW
                     ELMAAFLVQRGFQIYGVIQNPVDPGLAALIERYRKNIGGKPIPKQFMLRDPIKRLREG
                     NMVVVAGDQNWGGKGALRIPFFGRDCGTAGGPAGFSLIADVPLLPAVAFRTGSFSYRL
                     EIGPAIKKPVTGTRDEKIIEMTAAACKWNESIIERAPEQWLWMHRRWRD"
BASE COUNT         5567 a         6432 c         6027 g         5391 t
ORIGIN      
        1 ggaagaggga gccgacgaac ttgtctttct tgacatatcc gcttccctgg aagagagaaa
       61 gaccaggatc agttgggtgc aaaacgttgc cgacaggctt tttattccct tcacggtggg
      121 cggcggaata ggctcaaagg aagaggcaag ggaccttatc gcaatgggag ccgacaagat
      181 atctctcaac accgccgccg tctccgaccc ctccctcatc ggcaggtgcg cttcgctgct
      241 gggctcacag gccgttgtcc tcgcgatcga cgcgagcagg agcccaaggg ggggatggga
      301 ggtttatgtc cggggaggtt cagccccgac agggatcgac ctggtctcct gggcgcagaa
      361 gggagagtcc atgggctgcg gggagatact cctcacatcc atggattccg acggaacacg
      421 gggagggtac gacctggaat gcatcgaggc ggtctcctcc tccgtctctg tccccgtcat
      481 agcctctggc ggcggcggaa ttccggaaca tttcgccgaa gcgatatggt ccggagcgga
      541 tgcggtactg gcggcgtcgg ttttccatta cggctccatc aggataccgg aactcaagaa
      601 atatctggca ggcagatcga tacctgtaag aacgaccggg gaggagaacc ttgtatgatg
      661 cagtacgccg atcagatccg ttttgacgaa aaggggctcg tcccgataat tgcccaggat
      721 gccgatacag gcgaggtttt gatgcttgcc tggggcaaca gggaatccct tgtcgagacc
      781 gaaaggctga aggagatggt ttttttcagc agatcgcgca acaggctgtg gcgcaagggg
      841 gagaccagcg gcaacaccct cgcggtcgtg gagatccgca gggactgcga caacgatgct
      901 ctcctcgctc ttgtaagacc cagtggacct gtatgccata ccggttcctt cagctgtttt
      961 tcgtctcacg gcgagaagtt tgcgggtttt tcctgtttcg cgggaaaact ctggagatac
     1021 cttctttccc gaagcaaggc ctcgcccgaa gagagttaca ccgccaggct tctttcccag
     1081 gggctatccc gtacggggca aaaggtcggt gaagagggtg tcgaaacggc cctggccgtc
     1141 gccacaggga acaccgaagc ctttattctg gaggcggccg acctggtcta tcacctgatg
     1201 gtctgctgcc tggccctcgg tgtatctccc ccgatgataa tggaagagct cgagaaaagg
     1261 cacgtggaga aaaaaaccga tgactgatac ggggctggtc ttcgatgtcg atggagtcct
     1321 tgttgacacc tccctgtcat ttccggcggt ggtttccgcg gccgtccaga tcggggcaaa
     1381 ggatatcctc ggccaccctg cgccgtttcg cctctttgac gacgagcatt acaacatcgc
     1441 ccgaagaagc cccatgttca acgatgatta cgacatagcc tgggttttcc tctcatggat
     1501 cgcttcgggt tctgccttac agagagaaga aggaccggta tcccgccttg actgggaaag
     1561 actcattgcc agctgcgacg ggagccccga ggaatggtgt ggatccgtct ttcaaagccc
     1621 tgtagaccgc ggctccgtca gaaaaacctg cgaggagata tatttcgggg aggagcagta
     1681 tttgcttttc cgtggcgttt ctcccgaaat tcgaatcggg gggctatgga aaagagagcg
     1741 gccctgtctc gaaaaggagt gggaaagtct cgatattccc acaggaatct acaccggaag
     1801 aaacaggcag gaactgcaac tggccctgaa ccttctcggc tgggacggtt tccctcccca
     1861 cctgtgcata actcccgatg aaggaatatc aaaaccttca ccccggggcc tgaagattct
     1921 tgaggaaagg ctgagtgttt ccaggatcat tttttttgga gattctgaaa gcgacagaaa
     1981 agccttcgag gcatacggga agggcctgtt cgtgccaata ggcccttcct ttgatagtga
     2041 aaggggaggg ttcccggata tccggactgc cctcgagttt ccctggatct cgaaacctca
     2101 gtgacgcccc tcgctctgtt ttatcagctc cgccactgct ttcccgtcgg aagattccag
     2161 atatttcaag gtgctttcgg ggaccatttc cctgacgaca tcccatctgt cggcctttat
     2221 catttccctg acaattgaag cggaaaccac ctggccatca ccaacggtta ccctcgatat
     2281 ctcctcgagt tcgacacctc ttgggggcaa cacttccttc atggcttcgt tgtaggaccc
     2341 tgtcacggga cagtagggct cggtaccgac aaaacgcacg gagatcccca gggccgggac
     2401 aaaaagattt gcaaaaaggg tcacgtcaag ccttgcctgg atcgaggcga tcttctttac
     2461 cgatgatcct ttcagaaaat aggaagggaa cgtagcagga gagacagcgt attcaccgct
     2521 tcttatcact gtaacgttct ccaggtgaga ggttccttcc ctgacaaggg caaaacggtc
     2581 acgaaaaggg aaagatgaca ggtctgtctc gaccactacg acatagagat gatcacacct
     2641 cgaggcgccc gtctccacaa ggtactggtg tcctttggtg aaaggattac agttcaccac
     2701 aacggcacct gccctgcttt tcgctcccat gttgaccctg ctgcccctga gataatccct
     2761 gaactggtct atcccgggct gccccatctc cagcagggtc gcaaaaggtt cgtaacgggc
     2821 aatctccctg aaaccgaaac catggaactt gtccgctgaa tccggtttgg tgaatacaaa
     2881 aaaatgcgtt aaccccgatg cccttccccg ctcgacaagt ctcgagatga tactcccgga
     2941 aagacccgcc tcctgccagt caggatccac tgccatcatc tttattacgt tgcccttcag
     3001 gcttccggtg cccacaagtt cacccgaagg gccttcggca aaaacggaaa catcaggcac
     3061 accttccatc gaaagaccga acctgcccaa aaacttctct attcctgcaa gttcagcgac
     3121 ggaaggtttt tctgtcagtc taaattcgat accgaacaag aaccatcacc ccggcgcgac
     3181 tcagcgcttg cggagctctc tcttcaggat cttgcctatg ctggaccttg gcatctcctc
     3241 aatgaattcc acgctacgag gcaccttgta gtgggccagt ttatccctgc aaaaggaaat
     3301 gacatccctt gaagttatgg cgggctcccc ggtgggtatg acgtaagcct tgacaatctc
     3361 gccgctgacg ggatgtttga cacctaccac ggcagactcc cggactcccg gcaactgctg
     3421 gataacggcc tccacttcgg tgggataaac attgaaccct ccaacgatgg cgatatccgt
     3481 gagcctgtca acgatggtaa ggtagccgtc ttcatccatc ctgaccacat caccggtgtt
     3541 gaaccagccg tccttgaatt tttgggcagt tatctccggt gccctgaaat atccattgaa
     3601 taccgaaggc ccctttagcc agagtgtccc ctctttcccc acgggcagaa catttccgtc
     3661 catatcgcgg atctcgacct cgtatccctt aaggactctt ccgacggtcc cgagtttcct
     3721 gtccttgagc ccttcgttga gggataccac gggggatgtc tcagtaaggc cgtagccttc
     3781 caggaccgaa accccgagga gctgttgggt cctgccgtca agtttctcgg ggaaacgatc
     3841 cccgccggtc gcaagaagcc tcagcgaagt aggtgcctcg gttccccttg ctatcgctcc
     3901 gagcatcagc gcgaccattg tcggcacggc tatcatgacc gaaacatcgg cagcctttat
     3961 tgattctatg gctgtctcgg gtggcatgaa ggtggggaca agggcctggg ggaagccacc
     4021 cagaagggga agcaatccgc tggtagtgaa gccaagggtg tggaaattgg gaaggacatt
     4081 gacgaaaata tcatcgtccg caagatcctt gaaatgctct atcgatctgc tgacattgtc
     4141 gtggaggttt gaatggctga tcggaacccc cttgggcagc ccggtagtcc cggaggtgta
     4201 gaacaacacc gcgatatccg gatcggaggg ctcgcttttt ctggacttga aggaaggaag
     4261 ggcaccgtcc agtgggacag ccattgccgg tatgcctgaa gcggatattc cttcggccag
     4321 ctggtccatg ccctgaggaa caaaggcggc ggacacgtcg gagtgtcgca ggacatccat
     4381 ggttgcggac ataccggcct gcagattgag gggtgtcagg gtgcctccgg ctttccacac
     4441 cgcgaccatc agagcgagaa atgcgggaca gttaggcatt agcgccgcga tcctgtcacc
     4501 ttctccaaaa ccgctttctc tcagcttttc ttcatactgc attatgagat ccgcaagaac
     4561 cttcctcgac caccatgcgc ctctccacca gacggcacgc tctccattcg attttttgca
     4621 ttttgagatt atggtttcct caagtctgac tttcacgcgt tttccctccg aataatagga
     4681 taagttcaac cccctgaagg gggataatgt attatgcaga ctattttaac cgcaaaatgc
     4741 tctcccggac tccctgtcag catgaaaggg caagaaaggc ctcccatgcg gacgcgagag
     4801 ggatcagttc ctccggcacg tcgtaacggg ggttgtgaag tccgaactcc cctcctgtac
     4861 ccatgagcat aaaacatgag ggcgctttca aagaatagaa agcaaagtcc tcccccgcca
     4921 gcagctggcc ggtcatttca gtaacagtgg aaacgttttc ggagaagaag gtcctgcttt
     4981 tttccagaac aagggacgta atttcgggat cgttaactac gcaggggtag ttcctgttgt
     5041 actccaccga ggccgtcgcc ctgtgagccc tggctatgag agggatcatc tcttcgagcc
     5101 ttccctggat gaaatccctc acttccggtt cgtgagccct gagggtcccc cacatatggg
     5161 cacttcccgg aataatgttg taagcctctc cggcctctat ccttccaaag gagatcaccg
     5221 cagaagtctg gggatctttt tccctgctga ttatgtcctg gaccgaaaga agaatgtgag
     5281 cggcaataac cgtgggatcc actccctgat gaggggatgc tgcatgggag gtcgaaccgt
     5341 ttatcataat atggatcctg tcggaaaggg ctgtcatgca tccttttcga gtggccatct
     5401 gcccaaaggg aatgctgggc cagaaatgaa gaccaagaac gcacttaacc tggaacttat
     5461 cgaaaaaacc cgcatctact atcttttttg ctccggcctt gccttcctcg gcaggctgga
     5521 aaagaaaaat aacgggcctc ctcaaagagg aagcccgttc cgaaagaagt ctggcagcgc
     5581 caagaaggga tgcaatatgg gcatcatggc cgcatgcgtg cattactcca gggaaacagg
     5641 aagaaaacgc cagcccgctc tcctcttcga tagcgagggc gtccatgtcc gccctcagaa
     5701 ccacagccgg aagacctgta tcttttccca aaaccccaag gaccgatgtc ggggagccga
     5761 atcccctgat aacctccata ccatccatta ctgaaagtgc atcaaccacc ctcgaggagg
     5821 tgaccgtctc ctcaaaggca agttccggaa actggtggaa ctctcttctc caggcaatta
     5881 tctcgtgaga aatctcccct gcccgggact tgagatcttc ggccaggtta aatccatcgg
     5941 cattaactgt catcttgggg atcaggcctc ctgtggtttt taatatgtca ggttttacga
     6001 cccataaaaa aatataatgc tgttagtgta tcatactgtc atgcatcgag aacacgccac
     6061 gaatcataag gaggtctccc tatgtctgtt ccatgggtct acatcagcga tctcgcgaaa
     6121 cacgaggaaa aggaagtaat tctcaagtgc tggatgtata acaagcgtag ttccggaaag
     6181 atccattttc tgcagcttcg tgacggaacc ggttttatcc agggagttat ggttaacggg
     6241 gaagtccccg aagaacaatt ctcggcggca agaaacctgt ggctcgaggc ttctttggag
     6301 gtcaccggca aggtacggcg cgacgagaga gcgccctccg gttttgaact taccgtctcc
     6361 gatctgcgga taatccacaa ccccaccgag gagtacccca tcgggaaaaa agatcacggc
     6421 atagattttc ttctggacaa ccgacacctt tggctccgga gcgcaaacca gctggctgtc
     6481 atgaagatca gggagaggat aatctggtcg gcgcgggaat ttcttcacaa cggcggcttc
     6541 atgctggttg acagccccat cctcacagga gccatcggtg agggcgcttc cggtcttttt
     6601 gaggttccct atttcgatat gggcaacgca tacctcgccc agacaggcca gctttacgca
     6661 gaagccggag cagccgcatt cggcaaggtc tatacctttg gtcccacctt ccgggccgaa
     6721 aaatccaaga ccagaagaca tctgacggag ttctggatga tcgagcccga ggtggccttc
     6781 tacgatcaca acgacaacat gaaactccag gaggaccttg tatcctacat cgtcggacag
     6841 gtcctgaagc actgcggcag cgaactcgca accctcggca gggacattgc ccccctcgaa
     6901 agggtcgttc ccccgttcca tcacatcacc tacgacgagg cggtaatact tcttcagaaa
     6961 ctgggaagtt ccatcaccta cggtaccgat ttcggtggag aggatgagac catactaact
     7021 cagcagttcg acaggcctct cttcgtggaa tgctacccca agaaggtcaa ggccttttac
     7081 atgaagcagc acccggaaag ggaggacctg gttctgtgtg acgaccttct ggcgccggag
     7141 ggctacggag agataatcgg cggatcccag agagaggact ccctcgaact gcttcatgca
     7201 cggatccggg aggagaacct tcccgaggaa tcctattcct ggtacctgga ccttcgcaga
     7261 tacggcacct tcgtgcacag cggcttcgga atgggtatcg aaagggttgt ttcgtgggta
     7321 tgcggccttc cccacataag ggagacgata cccttcccga ggacaatata cagacttaac
     7381 ccatgacagg agtgatcccc tttggcatcg tcaaaatgga taccctacaa ggactttgac
     7441 ggtaaacccg aatatgaatc aaggaactgg cttatcggca gcggaaagat aggaggaaag
     7501 gcaaagggac ttgccttcgc ttttgagcat atccaggggt ccgaactcgc agaagaggtt
     7561 ctactgcctt ctaccacttt cgttgtcact acggagccct tccatgattt tatcaacgat
     7621 aacaagatgg actggataca cgacgaaccg gatctggaaa agataattct ggcattttca
     7681 cagggccaca tgcgggaatc ctttgtgaag gacctcgaca gaatactggg aactcttcac
     7741 gatactcctc tggctatacg ttcaagttca ctattggagg actctcatct actctccttt
     7801 gcgggcaagt atttcaccac ttttgcggca aatgtttttg acatggagtg gcgccgcagg
     7861 gaactctccg acggcatcaa gaccgtgtgg gcatctctgt acaaccaggc cgcaagggcc
     7921 taccgagcaa agcacggctt caccgacagg gacgaggcca tggccgttct ggttcagcca
     7981 ctgataggtc atgaacatga cggcctcttt tatccggaac tcgccggtac cattttttcc
     8041 aaggtttacc gcagaccatc ccccaggata aacaaggaac agggggttac caggttctgt
     8101 ttcggtcttg gaacaaaaac tgtagaccgc tcatctgcaa gggtgattta tctttctcac
     8161 ccgaatatgc gccccgaggg caatcttgcc agggaagtcg cgaggtacag ccagaactcc
     8221 ttcgactacg tggatcttga gttcggggca ttcgtctcgg gagacctcaa gtcccttcta
     8281 cccttcgtta tgcagaacca caagttcgct acatcctttg tggaactata cggggacaac
     8341 agcatgtact gggccggttc cagcccgatg atccctacca cacccttctt taccttctct
     8401 gaactaccca aacgctgccc ccatttcctg gaaaccgcaa agaagatctc cgctcattac
     8461 gagaccgaaa tgggcatacc aatagacatg gagttcacct atgaaaccct cgaaaaaaag
     8521 atgaccctgg ttcagttgag acccctgtcc tcttacgagg aaatggcttc cgtccgaatt
     8581 ccaaaggtgc ctgaagaaaa aattcttctc aagggatcga gaatggttag caacggccag
     8641 attgaaagaa ccgatcacat agtctacgta gaccccttca gttacggaag cagggacaat
     8701 tttttcaaga tcgccagggc cctgggaaag gtgaacgaca gcctcaaggg aacaagatac
     8761 atcctggcgg gccccggacg ctggggaagt gtcaacccgg atctcggagt ccccgtggac
     8821 tacgcggagc tctgcaactg cggatgcctt gtggaacttg gcatcgtaga gaagcacttc
     8881 acgcccgaac tctcctacgg gacccatttc ttcctggatc ttgacgttga caacatcctt
     8941 tatcttcctg tttttgcagg ggacgacgcg gacatcttca acagggaatg gttcgagaac
     9001 aggccctacg aaactgggca agaggaggaa atcagaattt acaagggaga tttttcggtg
     9061 tttcttgacg gggacacaga agaaggtatc gtaatagaaa actgaaaaaa gggggcccct
     9121 gaggggcccc ctttttattt ccagcagatt gtgttttcta tggccagatc ccccggaaac
     9181 gcatggcgag tgtgatcctg tccaccgcgc gaacataggc ggcctgcctg aaggtaatgt
     9241 tcttctcttt cttgatcttc cagatctcct gaaaggcctt tgtgagtttt ttgtccagtt
     9301 tctgatgaac ctcttcctcg gtccagaagt ctccggtacg gccctggatc cactcgaaac
     9361 aacttactgt gacaccgccc gcatttgcca gaacgtcggg aacaacgaga atccccttgt
     9421 cctcaagaat tgccgctgct tccggagtaa cgggcccgtt ggcaagttcc agaacaacct
     9481 ttgcctttat gttttttgcg ttttcagcat tgatcacgcc ttcgagagct gcagggacga
     9541 gaacgtcaac ttccagttcc agaagttcct cgtttgtaac accatcggtg cccttcatcc
     9601 ccttgatgct tccagtctca cgctttaact ttgaaacttc ctcgtaggat attcccttgg
     9661 cggagtatgc tccgcccctg ctgtcgcacg ctgccacaaa attcagacca accagatctc
     9721 caagtttgtg agcataactt ccaacgtttc cgtatccctg tacggccgcc gtaaggggtt
     9781 tacccagaag accaagttcc ctgagagtct ccctgaggac atacatgcct ccccgtgagg
     9841 tagcatcacc tctgccgagg gagccaccaa ccgctacagg cttacctgtg tagaccgatg
     9901 gttcgctgcg accgtggatc ttttcatggg tatctacgaa ccatgacata acctgggggt
     9961 tggtgtacac atccggcgct gggatatcta tatctgttcc cacgaaacga ctgatcccaa
    10021 gagcatagcc gcggctgagt ctctccagtt ccgtcatggt gagttccttt gcgttgacgg
    10081 aaactccacc cttccctccg ccgtagggca ggtctgcggt ggcgcacttt atggtcatcc
    10141 atgaagagag agctgctact tcgtcgcgat taacatgggg gtgaaaacgg acgccaccct
    10201 tgtagggacc aagggcattg ttgtgatggc ttctccaggc tttaagaacc ttcacggaac
    10261 cgtcatcgag tcttagcgga atggttatct caagaacctc tttgggctgc tttagtatct
    10321 cgagatactc tggttctgcg ctgatatgcg gcgcagcttc ctccaactgc tgtacgaaca
    10381 tctcatgcgg gttcagtttt gccataacaa ctcccccttc tctctttgcc ctcaaggtct
    10441 tgtacatccc tgtgggcaca atttgtttat atttataata gcatttttgg ttcacagcac
    10501 aagtgaaaaa ttaccgtcga aataggaaaa aaataaaccg ggatcaccca gaagggtaac
    10561 cccggttcat taaagaacgt atgctaaatg gtaaaatatt ttctagagga aaacgcctct
    10621 cattcggaca gcgtccgcaa cacgcatgat tgctaccata aatgccgcgc ggcgcatctt
    10681 gaccttcttt tcattggtga aatcccagac cctgtggaaa ttctctttca tgatgtggat
    10741 gagccgggtg ttgtactcct cgcgggtcca gaaatagcca ccaaggtcct gtgcccattc
    10801 gaagtaggaa ccgataaccc cgccggagtt ggcaagaaaa tcaggtacga tgaggatgcc
    10861 cttcttgtct aggatagcat cggcttccgg tgtgatgggg ccatttgcgg cttcgacaat
    10921 gtacttgacc ttgatcttgt cggcgttgtt cttgttgaca acaccttcga gagcggccgg
    10981 aatgaagagg tcggcatcga tgaagagcag gtcttcggca gggatcttct cgagtccggg
    11041 ctgagtatag ccctcaagga gtttcttggg gtgctcgttg atgtgcttga atgccttgtg
    11101 gatatctatg ccatccttgc agtggtaggc accggtaatg tcgctgatac cgacaacctt
    11161 cgcgccggcg tcgacaaggg taagggccgc gtaggttccg acattgccga aaccctggat
    11221 catgaccgtg gttcccttga cttccttgcc tacggcattg agaagctcta tggcacaggt
    11281 tgccacgccg taacctgtcg cctcggtgcg acccttggag ccccaaagac tgatgggttt
    11341 acctgtaaat atggcgggct cgagccttcc gcgcatcttg ctgatggtgt ccatgaacca
    11401 gaccatttcc tgtccacctg tgttgacatc gggagccgga acatcggtcc atgcgccaac
    11461 aattggttcg atccttgcag ccagagtcct ggcaacgcgc tctttttctc ccatggacat
    11521 ctcgaggggg ttgcactcta ctccgccttt tcctccgcca tagggtatgc cggcgaggga
    11581 acacttccag gtcatcagca tggcgagagc ctcgcactca tcctgacaca cgtcggggtg
    11641 gaacctgatg ccgccctttg ccgggccgac tgcggtggag tgctgcaccc tgtagccgtt
    11701 gaagactttc gtcgtcccgt cgtccataac gacaggaata gacaccgaaa gcttgcgctc
    11761 cgagttgccg aggatctcga tgagtccctc gtcaagcccg agttcttcgg ctgccccata
    11821 gaagttcctg agggcgatat caagcagcac gttctcggaa gttctcttac ccatccaaac
    11881 tgcacctcca cattcatgct tatgacccgg ggctccccgg gccatggata attggaaaac
    11941 ttgttcattt tggagcaagt tcgttaactt tagacgacct tctcttctgc ttctactata
    12001 tgtgaaatat atcccttgtc aagaggttgg tattatgtat acccgaaaac ccaaaaccca
    12061 ggcgcctgga accacaccta cacatttttc ataatatgga tatgacatcc ctgaaaggcc
    12121 ttggttacca taaaaacagg ggccgaatcg aaaaccatcc tggtcttacc gtcaggagat
    12181 cttgccggat ctgaggaaat gataggtgta tgcaagcttg ttgccgaaag aagatccttt
    12241 tataaaaccc gcctcttcgg caatcttctc cacagtactg gctctcagaa agtgagggtc
    12301 aaaaataact tccgtaacgg ataaaatacc gccgggcttg agggcccgga atatctcatc
    12361 gacagcactt ctcttttccg gtatctctcc tagtactgtt acaagaagag ccctgtcaaa
    12421 gctgtcactg gggaggatgc catccccgag tccccccttt atcagatcaa cattctcaag
    12481 accctcgaca atgagctttt tctttaccct ttcaagcatt ccctcctgaa tatccagcgc
    12541 ggccactcga ccggactccc ccacagccct cgcaaggggc aaagtcagac gacccgggcc
    12601 gcatccggca tcaagaaccg acatccccgg ctgaatatca agcaacataa tgatctccga
    12661 ggctctgtta gtccgggtaa aggggttatc catctcgacc atacggtgaa gccatacagg
    12721 acatggaagg gagaaccttt tagaggccag cctccataag agattaccta ccagaattat
    12781 tgcgatgacc cacaggataa aatacacagt tattcccccc ggaatccgaa atgggtgttc
    12841 agaccttgca ggtgagtaaa caaatgaacc tcgacaaaca aaaacatacc ggcaaaaaac
    12901 tctatgggtt ggtataattc cctctgtgaa caaaaaatgc tctgggaggg tgcgattttt
    12961 ggacaatctg attaatggct acagacagtt cctcaataat gcagacaggg gtctctatag
    13021 ggatctttgc aatggacagc atccgcatac gcttgtaata ggctgttccg acagccggat
    13081 aatcccggag gaaatatttt ctgtcggccc cggggaactt tttgtactga gaaacatagg
    13141 caacctttgc tgtaccgaag agccctctgt tgcttccgct atcgattatg ccatcaatca
    13201 tcttcatgtt aaaaggatcg tgatcctttc ccacggagat tgcggagctg tcaaggcgtc
    13261 tcttcaccct gaacacctcg aagaaaacag cataaaaaga tggcttgggg cagaaagctg
    13321 taacggctcc tctctcgagg aagcaatcaa atcctggggg atccgtcagc tgcagcggct
    13381 tgattctttc ccgtccgttg cagatgccgt caaatcgggg aaactggaga ctattctcct
    13441 ctattttgac ctggcctctc ttcgaattga cagatacaac ggtaccggct ggatggcttt
    13501 tgatcagact tcttgaaaaa atctggcgat tgcccaaatg gaaacccatg aaaacaggag
    13561 agattgccgc tcctctccag gcatgctcgc cgatctggaa cactcacctg gctggggaaa
    13621 cctttgcgag tagtttccac aacgaccttg aggcatcatc ctgagcgggg gcttgtcatt
    13681 ctgtgagcag gggtttgtca ttctgagcag agcgaagcgg tatccgcaag caggcggacg
    13741 ttccgggcac tttgcccgcc ggcggaaatc tggtttaggt gccgtgaatc gtgaatcggg
    13801 aatcgggaat cggaaaaaaa caacccggat cctcacgtca cttcgttcct caggatgaca
    13861 gcaggagcaa aggcgtgaac ggtcaacggt gtgcaaaggc aagggcgtga gaggttagac
    13921 gtgagaagtg agacgggaat cgaaggggcg tcaggaggct ggagacggga gactgggaag
    13981 gcaagagcaa aagtgtgaac gggaaacgga aggggcaaac cccggttttt tgcaaaggat
    14041 ggttttctct gcggggcacc accctctgcg tccactgtgg tgagaaaagc cgtgtcaaaa
    14101 aaagccggga gcgttttacg ctcccggccc ctttattacc ttgacctacc ctccgcagcc
    14161 accgcgtttc atcgatgtag cggacatgac ctcaatattg gctgcccaaa ggaaacctgt
    14221 ctgatcgacc ctgacaacgt tgtcgaggaa ctgcatctcc tcaggcaccc ataccgtcac
    14281 accatcactt gcaagggcaa tatatccatt ctctgccggg ggcttgccac tctctacgag
    14341 agcagcgttg cgggcccctc aaccggcacc aagtcttctt actattacaa agccttcccc
    14401 gccggaatcc ttgatcttgt ttcttgcttt ttcgctaagt tccagtttaa gatcactcat
    14461 caatataccc cccgtagaga aatattctct gttatctcgc tcattatacc ctacggtcgt
    14521 ataaacgcca accttttctt gcaagtacat tcatgaaatc ttctggagga acagccagaa
    14581 acttcttacc cgagatatgt ccaaggggtt cgtccatttc atgaaattcg atgcttctgg
    14641 agtggagcat cggtctgcct actccgtttt ttctccactc atcgttcttc ttcttgtctc
    14701 cgtattttat atccccgatc accggaaatc ctttggaagc caggtgaacc cttgcctggt
    14761 ggggtctgcc ggtatgcagt tcgacgagaa gaagcgacat ggtaccatcg ccgtccattt
    14821 tccggaactt tgtcagtgcc ggaaggcctt ccttgccatc ggttgtagca atgttccctt
    14881 cccctgtctt gaggataggg gtatttatct ctccctcatc gggggcatcc cctgcaacga
    14941 gggccaggta ggtttttttg actcttcctg ctctccatgc ctcatgaagg gaccgaagtc
    15001 ctttacccgt cagggcaagg accatcgctc cagatgtgtt tctgtccagt ctgtgggcag
    15061 gaaccgggga aaatgacggg tcattccagc ctatcgttga tgtcaccctc gatataacgc
    15121 tctctccacc ctttgtgtcc ggctgtgaaa ggagattgga aggcttgttt accagacata
    15181 tatcccggtc ggcatagatt atctctatga gggatggtcc gggccccttc ctggcagatg
    15241 aaagcggctg acggagagcg atctcgtcat cccaggggac atccaccagt tgcccttccg
    15301 ccgcgtgcaa agagcattcg acctttttcc cgtcaaggcg gacaagccct tttctgaaag
    15361 ccctcatcac cgctccaaga ggaagatcag gccagatctt ccttatgatc ctgtcgatcc
    15421 ttctcccatc gtcatgcttc gaaaccttga atgactggga cattatcaga caccaccctc
    15481 ttctgcgggt tttctccgcc agaaccttcg acctgccgag gggtggaagt ccgagatcga
    15541 cggttctccg ttgccgcttt ctggaaatgt tttccagcag aacattatga aatccagttc
    15601 gtccgcggaa gagacgacca ttgcttccgg tgtggctgga agtaccggtg agccgtattc
    15661 ctttcggcca tggtgactga caaggatgtg gcctatggca tcagcagttt ctttgtcaag
    15721 cccgaattct ccggcaagcc ttgagaatag ggaataaccg agggcaatat gatctatgac
    15781 ggtccccgga actgtcatgg acggaaaggg gttcagcctg taggactcga tcttaccaag
    15841 gtcgtgaagg agccccccgg cggcgacgat atccagatcc atgtcaaccc ctgaatccct
    15901 gtaggtctgc cccagggcaa gagccccctt cgtcaccgaa agggtgtgtt ccagcaggcc
    15961 gtgtacataa gcgtgatgat gagatactgc cgccggaaga tcgcgatagc tttcccagag
    16021 gtctcccgat aatacctttt caagaaatgt tcttaccacc gtgccacagg atccgatgat
    16081 ctcgtggagt tccttctcca ttttttcaag ttccatggga gaggactgaa tgaatccgct
    16141 tacgggatat tttttggggt ccagaagata caactccaca aactggtact gctgcttccc
    16201 ccggaattcg gaaacagtac ctactgcccc cgctacggag cctatgaaaa gcccgggtat
    16261 gtccgaggat gccggttcca gggggatttt ttcacccgtt acggtgtccc accatttccc
    16321 gtcggaccac acctttgcat caatagaacc ctccgaatcg atcagtgaca cttcccagaa
    16381 aggacgattg tttttatctg tccttccctt gatctgctgg atcaccccga tcattctaaa
    16441 acgttccccg gggttcagtt ttttaagctg ggatgtagtc aaagtctgga tctctgtcaa
    16501 cgaaaacact ccttaatatg atccatcgtc acccataaag aaatcggaac cgttccagac
    16561 gggatcactc atgctccata cttggccagc cgccccgcgt gagcaagcat aagccccatc
    16621 agtttactgc ccggcattaa tccaaggctc cccagcgaac agcttctctc accaaactgc
    16681 gatgttgtgt cgggccggac aaatctgctg cagaccaaca ggggcgaagg atgccacgaa
    16741 tggccaccca tcatggccgg agtactgtgg tcccccgtta cggccagaac atcggggtcc
    16801 agtgacagga ggtcgggaag aagagtgtcc actttttcta tgacctcttt ctttgcctcg
    16861 aaatccccgt cctcgccgcg actgtcggtg tattttacat gaacgaagaa gaaatcgaaa
    16921 gaatcccagt tttttctgag ggactggaaa agagattcta ctgtcttccc tgcatcaacg
    16981 acatccattc ctactatcct ggccaaacca cggtacattg gataggtggc aagggccgct
    17041 gcccttatcc cgtagagatc cggaatgagg ggtatatcag gggtaccgga tattcctctg
    17101 agaagacacc cgttcgcctt ttcgttgccc gaaaggacct cgcgcacctt ccgaatgaga
    17161 gagtttatta cagttgctgt ttttcttcct gaatcgtcga ggggctctgc ccagaccatg
    17221 ggctttcctt cgatctgggg gtcggcatcg gttaccttct cgccaagctc ttctccggaa
    17281 agcaccaccg agaaccggtg ctcgagcccg gggtaaaatc ttaccgaaac tccatcgatc
    17341 tcccttatgg acctgccaag gagagaggtg atggaggaac tggactccgt atctatcctt
    17401 ccggctcttc gatccaggat aaggtctccc tcaccccagg ttgaataatt tccccgggca
    17461 catatctctc cggggaggac ctccgctccg gttcccatcg cctccagaat acccctgcca
    17521 ataaaatgat cccggggatc atatccgaag agggataggt gtcccggacc gctgccggga
    17581 gttatacccg tatcgacgat ctcgagcagc cccgtctcac cgcgcagtgc ggccatgtcc
    17641 aggttagggg taaatgccga ctccagttcc gtgagaccct ccaccgacgg gagaccgcca
    17701 agtccatcca ttaccagaag gaccattttt gtatcgctcc tgaccgccag ttcccgaagc
    17761 atttcaagcc ttcctttcaa ggcaggacca cccccatgat tttggagata tcaagactct
    17821 agaaccttac cccctgctga tcgggttgtc cattacggat cggggcagta actcaaagat
    17881 aggccttttt gcggttgaaa ttttgtggcg ataaaggaaa atcgttataa taattacaaa
    17941 cctccagagg agggatataa aatgaagagg atcattctga ggatatctct gatagccttg
    18001 gtagtctctc tcgcggcatc tcccgtgttc gcagcgctga gtccggagag cgttacaccc
    18061 atatgggcca acctcacccg caccgccggt ctcactgacg cgggtcctgt caatatcgag
    18121 aacaaaaagg aacccaatgc ctgggtttct ttctcggcta acaaatattc ggtacacgcc
    18181 accacgggtc tcctggagac cctgaaggac gagcatgaac tggccggtgt ccttgctcac
    18241 gagatcgggc atatcaagtt gggtcattac aacgaatcaa tcaaccggag cctgctctgg
    18301 ctgcttctct acaaggcgct gggtgaaaag aaggtcggag gggtggacgt ccttggcgcc
    18361 gggatagttc tagccgaagc gggtttcagc cgcgaacagg agatcgaggc cgatgactac
    18421 ggcgttcatc tcgcggcaaa ggccggttat gacccgtggg gtctgtacaa cgctttcaac
    18481 agcatgaaaa aggcaggcta caaaacatct cccagcggtt tcaactctca cccgccgacg
    18541 gagagacgca tgatccacgt aaaggcaaca actgaagcgg ttgagaaaca gtatccccga
    18601 aaaaatccat aactggctct tcttggcgat agcgtgatag gatacaatgg gagcggggat
    18661 gatctccccg ctcttttttc atccatatat caagggagga gtagccattg agcgattttt
    18721 ttgatcctct tttcagcagg tccgcaaaga acatcaagcc atctccgatc agggagatcc
    18781 tgcacctcgt aagaaaaccc ggcatgatat ccttcgccgg cggaatgccc gaccccgaga
    18841 tatttcccat cgaacaattc caggaagcgg caaatataat aacccgcgag ggcaaagata
    18901 ttatgcagta tggcacaacc gagggctatc cacccctcaa ggatttcatt tccgaatgga
    18961 cagccccaaa aatgggaaga aagttgcagc atgaagaaat tcttattacc gccggttcaa
    19021 gccaggtggt cgacctcctc tgctgggccc tgatcgacga gggagattat gtggtctgcg
    19081 aagaccccac ttttctgggc gccactggca ccatgagaaa ccacaaggca aggttcctta
    19141 cggtaccctg cgacgagaac ggcatgcagg tcgatcttct ccctgacctg atagaaaaaa
    19201 cccttgccga aggcggcagg atcaagttca tatacacgat cgtcaatttt cacaatcctc
    19261 tcggatgcga cctgtccctg gaacgcagaa agaagctcct cgatatcgcc aaaaggtacg
    19321 ggatcctcat tctggaggac gacccctacg gatacgtgcg gttcgatgga gaggacattc
    19381 cgtcaatttt ttccatggac agggacggca ttgtcctttt tgcctgcagt ttctccaaga
    19441 tccttgctcc cggcacgagg gtggcatggt gcgcgggaga caaggacctt atacgaaaaa
    19501 tgacggtctt caagcagggt acggatgtat gcaccagcgt tgtcgtccag gcaatggtct
    19561 gggagtactg caagttaggg cacctggact ccttcctgcc gcggatcatc gaccattacc
    19621 gcaataagag agacagaatg gaggccgcct tccacgccca tctgcccctc gatctgatct
    19681 catggcagaa accggcaggc gggttctttt actggatgac gactcccggc atccgcgcgg
    19741 acaggcttct ggaaaaggcc gtcgacaagg gtgtcgcctt cgttcttgga agcgcctttt
    19801 accccaccga ttcgggtggg gacaacaact tcaggatgtg tttcaccttc gcctctccgg
    19861 aaatgaccga tacaggagtt aagaggctgg gtgaagcctt gagagagtta gcctgacatt
    19921 cagcccagga caagcagagc atatgacagg gaggagatcc aattgagcgg tgtctgggaa
    19981 tcgaacttca gcaacaaggc catgaacgtg cggccttcgc cgatccggga aattctgagc
    20041 gttatcaagc agccgggaat gatttctttc gcgggcggga tgccggcccc cgacgtgttc
    20101 cccgtggagg agttccacct gggagcagag gcactcctga aggacgggaa gaaccttttg
    20161 cagtacggaa caactgaggg actgcctgaa ctcaaggaat ttttgtccga atggaccgca
    20221 ccaagaatgg gcaggaaaac agcccttgac gaaatgcttc tgacaaccgg ttcacagcag
    20281 gttctggatc ttatgggctg ggccatgatc gatcccggag attatgtcat taccgaagac
    20341 ccgtcatacc ttgccgccct cacggctttc cacaaccacg gagccagatt cataggcata
    20401 cccaccgacc acgagggcat gatggtggac atgttgcccg acaagatcga gaaagcccgt
    20461 cgcgagggca agaacatcaa gttcatctat accatagtca atttccataa cccgggcgga
    20521 tgcaccatga ccatcgaaag aagaaaaaaa ctggtcgaga tgtctcacaa atacaatatc
    20581 ccgatcttcg aggacgatcc ctacggatat gtccgttttg acggtgaaca cctcccctct
    20641 atcttctcga tggatccaga tggcggtgtc atatacgctg gatcattctc caagatcctg
    20701 gcaccgggaa cgcgtattgg ctgggcaaca ggcaacaagg atattatccg gaagctgacg
    20761 gtattcaaac aggctgtcga cctgtgttcc agcccgataa cccaggctct cgtctatgaa
    20821 tactgcaaga tgggttacct ggacaaacac ctgcccaaga tcatatccaa ctacaggacc
    20881 aaaagggatg ccatggaagc cagtttccgg aagcacctgc ctcttgagga gatatcctgg
    20941 gtcaaacccg aaggcggatt cttcttctgg cttgacatgc ccaacatcga cgcaaagacc
    21001 ctttttgaca aggccatcga gaagaaggtc gcttttgtga cgggcacacc cttcttttcc
    21061 aacggaggcg gagaacacaa cgccaggatg aactacactt tctgtcagcc cgacatcata
    21121 gatgaaggcg taaagaggct tggagaagcg gcaagagaga tactggaggg atagtagagc
    21181 gaaatgaacg gactgatcgc cgcaatatcc accgcatggg gagaatccgg aatatctgtc
    21241 gtacgagtct ccggaaaagg ctccagggat cttgtggacg gttttttcag aggcggggtt
    21301 tctcttgggg aaaccccgcc ccgtttcatg aggaacgggt tcatcactga taacgacgga
    21361 gccgagatag acgaggtcct cgccgtttgg ttccgggaac ccaagagcta caccggggaa
    21421 gaaatggccg agattcaatg ccacggtggg accgtcgctg ccaggagatg cctcgaacgc
    21481 tgcatctccc tgggggcaag aatggctttg ccaggggaat ttaccagaag ggccttcatg
    21541 aacggccgca tcgacctggc acaggcagag tcggttctcg gtatcatcag ggccaggtcg
    21601 gaccggtctc tgaggtcggc ggcgaagtgt cttcagggtt ctctttccgc gagggttaaa
    21661 aagatagacg aggaattgct ggaactctcg gcccttggag aggcttccct cgactacccc
    21721 gaggaggata ttcccaccct ggagatggat ttttttatca aaggtctgtc caagacgggg
    21781 gagaaaatca ggacactgct ctcctcggca agatcaggcc ggttcctgag agaaggagtg
    21841 agagtagcca tcgcggggag accgaacgtc gggaaatcct cactgatgaa cgcctttctc
    21901 caggaatccc gatccatcgt tacttctttg cccggaacga cccgggatat catcgaagag
    21961 gtcataaccc acaagggtat ccccctccgg cttgtggaca ccgccggtat aaggcttccg
    22021 aaggatccgg cagagcagga gggtgtggag cgtgccagag aagccgtaca aaagtcggac
    22081 attcgagtct gggtcataga cgggagtgaa cctttgagcg acgacgacct cgctctctcg
    22141 acgagcctcc agggcacatg tcacgtggtc gcgctgaaca aatccgacct gccccaggcg
    22201 gtaaatgtca ccgatatcat taagatcctt cccggtgcgg acgtccgggt catttccacc
    22261 gtttccggtg agggtattga atccctcaag gaccttatag tcctctcgtc tgcgggaata
    22321 acctcgatgg acgaagacct taacaccaca gagaggcaga tcactgaaat gtcaatggct
    22381 ctcgatcttg ttataaatac catggattct ctgacggcgg gggctccggt ggacacagcc
    22441 ctggaaactc tgtccgaagc cagatcctgc cttggaagaa ttatcggcgt tgacgccgat
    22501 gaatcgctta tggaaaaggt tttctccagt ttctgtattg ggaaatagac atttaccaaa
    22561 ggaggtatca caagatgccg ataatccagg ttcatcttct tgaaggacgt tcgggagagc
    22621 agaaaaagaa actggtcagc gagatgacag gagctgtatg ttcttctctg gacgtggttc
    22681 cggagcaggt ccggataatt ctttccgaga tgtctccaga agattactcc gtcggaggta
    22741 ccctgttctc agataaaaaa aagtaggatc ctcctgacga agggtttcag tcccgccagc
    22801 gccggtgcat ccagagccac tgctccggcg ccctttctat aatgctttcg ttccacttgc
    22861 aggcagcagc agtcatctcg attatttttt cgtcccttgt tcccgtcacg ggtttcttta
    22921 tggctggtcc tatctcgagt ctgtaactga aactccccgt acgaaaggcc accgcaggca
    22981 gcaatggaac atctgctata agagagaatc ccgccggtcc acctgccgtt ccgcaatccc
    23041 tgccgaaaaa ggggatcctg agggcacctt tcccacccca gttctgatcc cccgcgacga
    23101 caaccatgtt gccttccctg agacgtttta tgggatccct caacatgaac tgtttgggga
    23161 tgggtttgcc ccctatgttt tttctgtatc tttcaatcag cgcggcgagc cccgggtcca
    23221 cggggttctg tatcaccccg tagatctgaa accctctctg taccagaaac gcggccatca
    23281 gttcccagtt cccaaaatgg gacatcagaa ggaccgcgcc tttcccttcc tctcgctgcc
    23341 tctccaggag ttccatgccc ctggtctcct tgaaccactc cactacctgc tccggaccgt
    23401 tctggacgac caggtat
//
LOCUS       DAOTIQ010000027        22168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2787_length_22168_cov_7.622213, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000027 DAOTIQ010000000
VERSION     DAOTIQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2787_length_22168_cov_7.622213"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="P5541_04965"
     CDS             <1..366
                     /locus_tag="P5541_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71364.1"
                     /translation="WEKRFKSTARLASTFETACEAMGLELFVREKQFRSPALTSVIIP
                     SGKGQTVKEALGVLGIIASEGQGEGDTCLRFAHYSPGGWPEVSLLAGSLFGAARDCGI
                     DVDPGFIKEAWKTWNREGR"
     gene            363..2015
                     /gene="serA"
                     /locus_tag="P5541_04970"
     CDS             363..2015
                     /gene="serA"
                     /locus_tag="P5541_04970"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRV71365.1"
                     /translation="MTMYKVLVTETVSEAGLELLRKDKDIKVEVRTGLSREDLLEAVS
                     DVDGMITRSGTPLDAEILGAAKKLRAAARAGVGLDNIDLAEASRHGVVIINTPTGNTL
                     AATEHTMAMMLSLVRRVPQAFNSVTSGEWKRSRFVGRQLSGKRLLIIGLGRIGTQVAL
                     RCRAFGMEVTAFDPYISAEKAERAGVELVDDLAGGLSLADIVTLHVPLTRETRGMIDG
                     ETLKTCKRGAYIINCARGGLVDEETCAENVRSGFLSGAAFDVFDGEPVRQDHPLMAED
                     IRDRVVLTPHIGANTEEAQSAVATIACSNLLATLKGKPCENAVNLPFVEQTLSDGSRA
                     FLSLARKLGFLAAHLVREPVKDIRITLRGPLFSPEDDPICFEIPYHFSPFSVAGLKGF
                     LEFSHGPEVNYMSAPLIAADKGIRVEETRTAGGTWKNQIDLSLSVPGSRENVTVSGTV
                     TEEGKQRVVNIGGYWIEFVPEGTVLLFSNHDRPGVIGKVGTLLGKAGANIANFALGRR
                     NGSGLAVGALQIDDDIPEDVIETLKTDVDLLWAVKVNFAEAL"
     gene            2016..2651
                     /locus_tag="P5541_04975"
     CDS             2016..2651
                     /locus_tag="P5541_04975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV71366.1"
                     /translation="MKRIFLIRHGETDWNREGRFQGKMDIPLNSLGREQAGAAAKALE
                     TAAIDRVVSSPLSRAFDTAQAAASRNSFSIEMHSGFREIDHGLWEGHTAEEVEKLWPG
                     MLGTWHSHPEKVIMPEGESLENVQNRAWTALEDVIAAPGERVALFSHDAVIKVLLCQI
                     LGCPLSSFWRFRVANASITLLEANAKRISLLVMGDACHLGSPWERKEQKGL"
     gene            3189..4163
                     /locus_tag="P5541_04980"
     CDS             3189..4163
                     /locus_tag="P5541_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRV71367.1"
                     /translation="MSGKRETDTLFECGGMQKRKDLLDSLGGLPEGAVEVKNSEWLYM
                     LEEETRNSLAIEGYFASEKELRAIISGKHTGAEILDYYRTAGSMYDLALQYLREGQTI
                     FDVPLVRHIHSELFREGRDSARGNFRKGKIIIKGAKVHPPEADVEQYVRCLVEIVKSL
                     RGRTGAIPFLARFHVLFESIHPFADGNGRAGRILMNYLAISLGLPPFVIKGLAPADRN
                     EYYDALEKGDTGFHEAFPPPDADKILSRLETGNFSRLEEMFCKSSIHSLNRVIAAMAG
                     KIEELLPLADLAKEMKVQPATLRKWVERDKMVAVMKNGKLYSNKRLVF"
     gene            complement(4299..4655)
                     /locus_tag="P5541_04985"
     CDS             complement(4299..4655)
                     /locus_tag="P5541_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRV71368.1"
                     /translation="MKGETSSMIAFDDFEKVDIRVGRVIDVQDFPRARKPSYRVKIDF
                     GEDIGIRGSSLQAKNDYSMEEMLGKLVVCVVNFEPKNIAGFLSEVLVLGVSHEDGGLS
                     LLEPGRVPVPLGARVF"
     gene            4788..5693
                     /locus_tag="P5541_04990"
     CDS             4788..5693
                     /locus_tag="P5541_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRV71369.1"
                     /translation="MANIILMIVCFSLGIALRKCGRLPQATPSVLNSYIIHVALPALA
                     LLHIHGLEFSAALLFPAAMAWLLFAGGYVFFRILGKTAGFSAGTMGALFLTGGLGNTS
                     FVGLPMIEAFYGRDYLGIGILADQLGSFLVLSTIGIALAAAYSSGKTSWGGIARKVLL
                     FPPFVAMVLAFALITVPFPLWLQSVLERIGGTLPPLALLSVGFQLRVGHIRDRIKPLA
                     AGLLYKLILGPALVAAVFILLLGARGTEIQVTVFEAAMAPMITGSIVAAEHDLDPSLS
                     ALMVGVGIPLSFATLPLWWWVLGAF"
     gene            complement(5690..6373)
                     /locus_tag="P5541_04995"
     CDS             complement(5690..6373)
                     /locus_tag="P5541_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRV71370.1"
                     /translation="MNTLVSMLAGFVIAFALVLLVRSIRRKWSGGAKGRETTIYSSIQ
                     QLRAIGQLSVFKVLTKEIVTEVDHTWGEIGKKYLSWILTKKKMAMIFEFVIDFRYDLR
                     SEDFQIIEEPGGEYLLRMPPCIYETSIRDIQFYDEQKSRLVPWLLPDLLNTLFGDGFS
                     EQDKNRLKDAAKRAAESQAATMIAGLGADAEQSARQTLQSLGRAFGADNMRFEFLHEE
                     QKAPEAVLS"
     gene            6697..7668
                     /locus_tag="P5541_05000"
     CDS             6697..7668
                     /locus_tag="P5541_05000"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRV71371.1"
                     /translation="MKKHKAISLFIMFIVLICLPALAEADPVIEAPALKLGTFNLRRA
                     PALAYQDEKISATLQITLDRLFLDLRNLTQGIVIVDRDKFFFTDAESRVSRMTHDGTQ
                     YIHRESPQGQIILPPGGSVRTGMVPLDNIWWNDQSKKYETITIKDEDGKEKTIKIPKE
                     RDKNDNRNWEIRELAPWPSGGRLFSSEEKYMKLYSGKRLSLLVTLLFNGHDTRYYVFN
                     FDFPVYVTAEERETAKTRSASLDQYLILGEKTKAGFIVIQGVASGSPAEKKGIKAGDL
                     LMEIDGISVTGMEVDSVREYFSKKTGENRSIIVVTGRKGIKNIAVLK"
     gene            7983..8339
                     /locus_tag="P5541_05005"
     CDS             7983..8339
                     /locus_tag="P5541_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV71372.1"
                     /translation="MPREIIFYTKPDGTRPVEDFLTSLQPEHLAKAMRVIDLLEQFGA
                     ALQRPHVAVVRFKKYAGLWELRIQSAGNASRILYFSVHKDIFVLLHGFIKKTDALPVK
                     ELEIARKRKENYLERC"
     gene            8342..8626
                     /locus_tag="P5541_05010"
     CDS             8342..8626
                     /locus_tag="P5541_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV71373.1"
                     /translation="MSIKPRTWKEVKKDLFRDPETASAYDELQEEFSFIASMIEARQK
                     KGLSQADLAARIGTTQGNISRLESGRHNPSMRFLRKVAHALEKELEVSFK"
     gene            8808..9107
                     /locus_tag="P5541_05015"
     CDS             8808..9107
                     /locus_tag="P5541_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRV71374.1"
                     /translation="MISATKKSGTTVDRKTTTMRVRVRPALKEETQEVFQNLGLTTSE
                     AFNLFLEQVRLRGGLPFPLEIPNHRTAQAMKDIRAGRSLTAADNVDDLWDKLDRE"
     gene            9117..9389
                     /locus_tag="P5541_05020"
     CDS             9117..9389
                     /locus_tag="P5541_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRV71375.1"
                     /translation="MPKPFFSAQFKRDRKRMLRQGKDDSRLKRIVRSLAEGILLEPRN
                     NDHSLSGDWCDFRECHIDPDWLLIYRMDDKNLYLARTGTHADLFKA"
     gene            complement(9951..11048)
                     /locus_tag="P5541_05025"
     CDS             complement(9951..11048)
                     /locus_tag="P5541_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRV71376.1"
                     /translation="MVNKSYTEYKPDYAIPPGDTLSDVLESKGMTQQELSTRTGMAPK
                     TINEIIKGKAPISADTALKLESVFGVSASFWLNLEMNYQEDMARIKAKKGLSADIEQA
                     KKFPYAEMAQKEWVPPTRKDEEKVINLRQFFGVASLERLEDMPFVASFRRCDTPKTSF
                     HALSAWLRKSVLNAEEIPAESFSLKALKGSIPLLRNLGKGSCTDFPDHFKRAREICRK
                     CGVALTIVPHLRHTSVNGATQWVSPVKAVVSLSFRYPYWDVFWFSLFHELGHMVKGHS
                     KKDIFVNFGNSDDDIREEEANSFAADTLIPPKQYQEFRNRGNFSDDAVEAFAKEMETS
                     PVILLGRLCKEQILSWDIFARSRFDRRLQPD"
     gene            complement(11038..11379)
                     /locus_tag="P5541_05030"
     CDS             complement(11038..11379)
                     /locus_tag="P5541_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV71377.1"
                     /translation="MDIRFRSRKLERACNEEATGQRQWGQNRARKVGQRLMELRAAGN
                     LLDFSKIPGTGFHPLHHDRQGQFAVSLGGNWRLILLPIENSSPCLPDGSVDLENISGI
                     RILEVVDYHGE"
     gene            complement(11720..12880)
                     /locus_tag="P5541_05035"
     CDS             complement(11720..12880)
                     /locus_tag="P5541_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV71378.1"
                     /translation="MEMQQVIRQLETISHRNRSSLPEPLRPFYGANPVNSRGALITGA
                     RGVGKTTFLLFSMSDDMIYISADNPLLAGIPLWDVGEAAFMSGFQGIVVDEVHFAKDW
                     SRDLKALYDSYPRHKIWASDSSSLVLREGVADLSRRFPITRVPLLSLREFAVLKGEKD
                     PGVIDPLSPDNGKIHEYLDETNVTGLFREYLEGGFRPFFTEGRYMDRLENVIGKMQFS
                     DIPFFVPQITDNHLRLMKAATGYLARSDVPVLNVERLCKEWGLGKAKLLQLFEVMETV
                     GLIRIARKENDNRVYSKGAKLFLGEPPMYSLFGGGLGVSREAFAVLAFQESGREIESV
                     RDERRGDFVVEGELIEIGGRLKKIKNADIVFRDDTELPGKGGYPLWLLGMGW"
     gene            complement(13070..13942)
                     /locus_tag="P5541_05040"
     CDS             complement(13070..13942)
                     /locus_tag="P5541_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRV71379.1"
                     /translation="MRKRTRDEYEQAIRNVQFLIQEHLDEKISMDDLARAAGFSMFHF
                     HRIFSAMVGETLGEHLLRLRMERAAFELVYSSRSIPDIAFSAGYETHESFTRAFRKRF
                     GGTPSGYRKEARCERGIGSPPGLRAVIQRQGGKPMEVRIEKFEPMTVVSVRYTGPYAG
                     CGEAWGKLCSNSRNSAAIGPKTLFIGISYDDPDITEAEKIRYDACMTVAEGFEQESGF
                     EKQAIKGGKYAVLRHTGPHDGLLACYRWFYGEWLPGSGCEPAAAPSFEIYRNFPEETP
                     PEKLITDICIPLEE"
     gene            14194..14484
                     /locus_tag="P5541_05045"
     CDS             14194..14484
                     /locus_tag="P5541_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV71380.1"
                     /translation="MTRDDIIRKIREHEKEIQDLGAERLSLFGSAVRDDLLPESDIDI
                     LVSFVMPADYRRYINLKFFLEDLLQRPVDLVMETALKPRIRKRVESEMIRVA"
     gene            14477..14818
                     /locus_tag="P5541_05050"
     CDS             14477..14818
                     /locus_tag="P5541_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRV71381.1"
                     /translation="MRDPELLLEDILECSEKIARYTKGITIENFRQNSMLQDAVVRNL
                     EIIGEAVRKLPEDFLEKHPDLPWREIAALRNILAHAYFGLDLEIIWTLAIEETPSLSN
                     QIESIIKNRSF"
     gene            14991..15272
                     /locus_tag="P5541_05055"
     CDS             14991..15272
                     /locus_tag="P5541_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71382.1"
                     /translation="MKTVSSRDLKNNPGILADSDVTVVYSRSKPMAVCVSVSEGEDVL
                     SLADAMLQMRAQRALELARIMARENGTDRIPDSEIDAEIRSLRAAQKKD"
     gene            15281..15694
                     /locus_tag="P5541_05060"
     CDS             15281..15694
                     /locus_tag="P5541_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRV71383.1"
                     /translation="MIWFVMDTNVLVSGFLRSNTPPGILLNGLVNRSFGLVLDSRIFL
                     EYRDVLARPKFCLPPAVTQPVLALINAKGHWISPTRYGGTLPDEGDRSFVEAALSAGV
                     PIITGNLKHFSSVPLLSVLSPAQALEAMREHKRGE"
     gene            complement(16135..17073)
                     /locus_tag="P5541_05065"
     CDS             complement(16135..17073)
                     /locus_tag="P5541_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV71384.1"
                     /translation="MKRVIVTGSNGQIGVELTPHLRKIYGSENVLATARRKVAGPVSE
                     GGPFELLEVRDGRRFSELIRSFKADTVIHLAGILSAKGEADPQGSWDINAGGCFTALE
                     CARENGAAFFFPSSIAAFGPTTPADDTPQVTIQRPTTIYGVAKVTGELLCDYYHLKFG
                     LDTRGLRFPGLISYEALPGGGTTDYAVHIYYEAVSKGKYTSYIAPGTQMDMMYMPDAL
                     NAVVELMEADPSRLVNRNAYNISAMSFDPEQLAASIRKVMPSFELSYDVDPVRQGIAE
                     SWPNSLDCTAAKKEWGFAPEYDLDGMTEDMLRRVKK"
     gene            complement(17070..18257)
                     /locus_tag="P5541_05070"
     CDS             complement(17070..18257)
                     /locus_tag="P5541_05070"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRV71385.1"
                     /translation="MAVPMKFLTEDLDAMKSAGLYGTIRTIESPQGAWVKIEGKKFLN
                     LCSNNYLGLCNDPRLVSKAKEYIDNYGVGPGAVRTIAGTMSIHLELEKRLAEFKGAEA
                     SIVLQSGFCANLAVIPTLVPSAEDIIYSDSLNHASIIDACRLSKAKVVRYEHSDMDSL
                     REKLEEYKGAPGKQLLVTDGVFSMDGDIAKLPEIVEICEKYGVIVVVDDAHGEGVLGR
                     GGRGIVDHFGLHGKVDVEVGTMSKAFGVMGGVVAGSKVLIDYLRQKARPNLFSSALTV
                     PDVAACQAAVDILESSDELVTRLWENGNFLKKELSAIGFNTGNSETPITPVIIGEAQD
                     AKTFSAKLFEEGVFATAIVFPTVPKGTARIRCMVSAAHSGEDLSQAVERFARVGREMG
                     VIS"
     gene            18422..19114
                     /locus_tag="P5541_05075"
     CDS             18422..19114
                     /locus_tag="P5541_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71386.1"
                     /translation="MDGAKELEKLAVRISGDISAPHHIANVLREGIYRGILEEGKPLH
                     QSQIAARLGVSPIPLREALRLLETEGLVEFRGYRGAFVTELSLEEARELYEMVAALET
                     SLMRTAFPGITRRTIEEAEQVLEKMEAVDDCIRWRDLNFLFHTLLYEPADRPLTLDMV
                     ARLRQKTDRTIRTHLDSMRKESELQHRNILEGVAAGDLERSVSALAHHLAYTSSDLQS
                     WMRSEQAGRKRV"
     gene            complement(19292..19546)
                     /locus_tag="P5541_05080"
     CDS             complement(19292..19546)
                     /locus_tag="P5541_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV71387.1"
                     /translation="MQVFTYSEARQKLSSVLDKAEKSGKVLIRRRDGRTFALVPEPAA
                     ASPLDVPWIDAQVTTEEIVSILRKQRGRKTAATRFLEKRS"
     gene            complement(19550..19942)
                     /locus_tag="P5541_05085"
     CDS             complement(19550..19942)
                     /locus_tag="P5541_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV71388.1"
                     /translation="MDIVIDASAILAVIMNEPERESIIEMTTGHTLLGPGSIPWEIGN
                     AFSSMLKQRRLSLEDACRGLEIFQTIPIRYLPVDMANAVSIAHGARLYAYDAYFLDCA
                     ARHAVPLLTLDRSLQRAASQIGIQVMGV"
     gene            20202..20501
                     /locus_tag="P5541_05090"
     CDS             20202..20501
                     /locus_tag="P5541_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRV71389.1"
                     /translation="MISATKRSGTTNDLKTTTMRVRVRPALKEETEEVFQNLGLTTSE
                     AFNLFLEQVRLHGGLPFPLEIPNHRTAQAMKDIRAGRTLTAAEDVDDLWDKLDKE"
     gene            20511..20783
                     /locus_tag="P5541_05095"
     CDS             20511..20783
                     /locus_tag="P5541_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRV71390.1"
                     /translation="MPKPFFSAQFKRDRKRMLRQGKDDSRLKEVVTALAEGIPLEPRN
                     NDHTLSGEWSDFRECHIDPDWLLIYRMDGGNLYLARTGTHSDLFKS"
     gene            20837..21064
                     /locus_tag="P5541_05100"
     CDS             20837..21064
                     /locus_tag="P5541_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRV71391.1"
                     /translation="MPVPTSVRLSEETEKRLSRLALETGRTKAFYIRKIIEDHLEDME
                     DYYLAAETLAKVRRGEEKVSGWDEVVSDLKK"
     gene            complement(21376..21648)
                     /locus_tag="P5541_05105"
     CDS             complement(21376..21648)
                     /locus_tag="P5541_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV71392.1"
                     /translation="MIRRLLITKKADSFLSRVPKKHSAQLARKIMMLLEEPRPADSIS
                     MKGSPFFRVDSGEYRIVYRFDDEFLYVSVIGKRNDGDVYKKAKRIE"
     gene            complement(21641..21898)
                     /locus_tag="P5541_05110"
     CDS             complement(21641..21898)
                     /locus_tag="P5541_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV71393.1"
                     /translation="MKTASATEAKQNFGRILEESLVEPVIIKRSGRVSAVLLSHREYE
                     RLLGIEESYWIARATDAESSGFLDEARSMKRLTGLISSSDD"
BASE COUNT         5266 a         6006 c         5853 g         5043 t
ORIGIN      
        1 tgggaaaagc gattcaaaag cacggcccgt ttggctagca ctttcgagac cgcctgcgaa
       61 gccatgggac tggagctttt cgtccgggaa aaacagttcc gttcccctgc acttacttca
      121 gtcatcattc cgtcaggcaa gggacaaacg gtaaaagaag ctctgggtgt cctggggatc
      181 atcgcctccg aaggacaggg agaaggggat acctgcctcc gcttcgccca ctactccccc
      241 ggtggatggc ccgaggtatc actgcttgcg ggaagcctct tcggagccgc aagggactgt
      301 ggcatcgacg tggatccggg cttcatcaaa gaagcctgga aaacctggaa cagggaggga
      361 agatgaccat gtacaaggtt cttgttaccg agacggtttc cgaagccggt ctcgagctgc
      421 tcaggaagga caaggacatc aaggtcgagg tccgcacggg tctttcccgg gaagacctcc
      481 tcgaagccgt ctcagatgtc gacgggatga taacccggag cggcactccg cttgacgcgg
      541 agatcctggg cgccgcgaaa aagctccggg cagctgccag ggcaggcgtg ggtctggaca
      601 acatcgacct tgccgaggcc agccgccacg gggtcgtaat aatcaacacc cctacgggga
      661 acaccctggc tgccacagaa cacaccatgg ccatgatgct ttcccttgtc cgaagggtcc
      721 cccaggcctt caactcggtg acatcgggag aatggaaacg tagcagattc gtcggccgcc
      781 agctttcggg taagagactc cttatcatag ggctgggacg gatagggaca caggtggccc
      841 tccgctgcag ggccttcggc atggaggtta cagctttcga cccctacatc tcggccgaga
      901 aggccgaacg ggcgggagta gagcttgtgg acgacctggc cgggggactc tcactggccg
      961 acatagtcac cctgcacgtc ccgcttaccc gcgaaacgag agggatgata gacggtgaaa
     1021 ccctgaaaac ctgcaaaaga ggggcataca tcatcaactg cgccaggggt gggctcgtcg
     1081 acgaggagac ctgcgccgaa aatgtgcgct ccggtttcct ctccggggca gctttcgacg
     1141 tattcgacgg cgagccggtg cgtcaggatc accccctaat ggcggaggat atccgtgacc
     1201 gggtcgtcct cacaccccat atcggggcca acaccgagga ggcacaatcg gctgtggcga
     1261 ccatcgcctg ttccaatctt ctggccaccc tcaaggggaa accctgcgag aacgccgtca
     1321 accttccatt cgtcgagcag accctctcgg acgggagcag ggccttcctt tccctggccc
     1381 ggaaactggg cttccttgca gcccacctcg tcagggaacc ggtgaaggat atccgcatca
     1441 ccctcagggg gcctctcttc tcacccgaag acgaccccat ctgtttcgag atcccctacc
     1501 acttctcccc cttctccgta gcggggctaa aaggtttcct tgagttttcc cacggcccgg
     1561 aggtcaacta catgtccgca cctctgatcg ccgccgacaa ggggatcagg gtcgaggaga
     1621 cccgcacagc cgggggcacc tggaaaaacc agatagacct ttcactctca gtgccgggat
     1681 cgcgggagaa cgtgaccgtc tccggcacgg tcacagagga gggaaaacag cgggtcgtca
     1741 acataggcgg ctactggata gagttcgtcc ccgaaggaac ggtgctgctc ttcagcaacc
     1801 acgaccgccc cggggtgata ggaaaggtgg gcaccctgct tggcaaggcc ggggcgaaca
     1861 tcgcaaactt cgccctggga cgcaggaacg gcagcggcct cgccgtgggt gcgctccaga
     1921 tcgacgacga tattcctgaa gacgtcatcg aaaccctgaa aaccgatgtg gaccttctct
     1981 gggccgtgaa ggtcaacttc gcggaggctc tctagatgaa gcgcatattc ctgatccggc
     2041 acggcgagac ggactggaac cgcgaggggc gcttccaggg gaagatggac atccccctga
     2101 acagcctcgg aagggaacag gccggagcag cggcaaaggc cctggaaacc gccgccatcg
     2161 acagggtcgt ttcaagccct ctctcccggg ccttcgatac ggcacaagcc gcggcatcga
     2221 ggaacagttt cagcatcgag atgcacagtg gattccgcga gatagaccac ggattgtggg
     2281 aggggcacac tgccgaagag gtggaaaagc tctggccggg gatgctcggc acttggcatt
     2341 cgcaccccga aaaggtcatc atgcccgaag gtgagagcct ggaaaatgtt caaaaccgcg
     2401 catggaccgc ccttgaagat gtgatagcgg cccccgggga aagagtcgcc ctcttctccc
     2461 acgacgccgt catcaaggtg ctcctctgcc aaatccttgg ctgccctttg agcagcttct
     2521 ggcgcttccg ggtcgccaac gccagcatca ccctgcttga ggcgaatgca aaaaggatca
     2581 gcctcctggt catgggcgac gcatgccacc tggggagccc ctgggagagg aaggaacaga
     2641 aggggctgta ggggaaagac aaggagacag gatgcaggag tcaggaaagc aggggcaaag
     2701 gcgtaaacgg tcaacggtca acggtgtgca aaagcaagag cgtgagacgt gagacgtgag
     2761 acgttagacg aaaaatgagt gccaaggtct ccttggttcc caaaaatggt tttctctgtg
     2821 tggtttcacc ctctgcgttc cctgtggtga gaaagttttg ggtcttgccc cgccccacac
     2881 aaagaaaggt tttctctgcg gggcttcacc ctctgcgtcc actgtggtga gaagggccgt
     2941 gaatcgaaag atcaaggagt caggagtcag gaaatctgaa gcaagaggct ttgggtgaaa
     3001 gacaaggaga caggtatcat acctttctct gcgttcgggc agagccaaag cctcccctaa
     3061 aattcgttcg ggcatcttgt cacgatggaa atatagagtt atcatcgtga cagtgaaaaa
     3121 tgccgataaa acaggccaaa tcatgaaatt ctattcgctt tgatgtgaca aagggggcta
     3181 aagccgtggt gtccggaaaa agagagaccg atactctttt tgaatgcggg ggcatgcaaa
     3241 aaaggaagga tctcctggac agccttggag ggctccccga aggcgcagtt gaggtaaaaa
     3301 acagcgaatg gctgtacatg ctcgaggaag agaccagaaa ctccctcgcc atcgaagggt
     3361 acttcgcctc tgaaaaggaa ctcagggcca tcatctccgg aaagcacacc ggcgctgaaa
     3421 ttctggacta ttaccgcacc gccgggagca tgtacgacct tgcccttcag tatctccgcg
     3481 aaggtcagac gatattcgat gtccccctcg tgaggcacat ccacagcgaa ctcttccgtg
     3541 agggaaggga cagcgcgcgg gggaacttcc gcaaagggaa aattatcatc aagggggcaa
     3601 aggtacaccc acctgaagcg gatgtagagc aatacgtgcg atgcctggtc gagatcgtca
     3661 aatccctgcg aggcagaacg ggggcgatcc ccttcctcgc caggttccac gttcttttcg
     3721 aatccatcca tccatttgca gacggcaacg gacgtgcggg aagaattctc atgaattatc
     3781 tcgcgatcag tctcgggctt cctccatttg taataaaggg ccttgctccg gcggacagga
     3841 acgaatacta cgatgccctg gaaaaggggg acaccggatt tcatgaagcc tttcctcccc
     3901 cggatgccga taaaatcctt tcaagactgg aaaccgggaa tttcagcagg ctggaggaaa
     3961 tgttttgcaa gagttccatt cacagcctga acagggtcat tgccgccatg gccggaaaaa
     4021 tcgaagaact tctgcctctt gcagaccttg cgaaagagat gaaggttcag cctgcgaccc
     4081 tcagaaaatg ggtagaaagg gacaagatgg tcgccgtaat gaagaacgga aaactctaca
     4141 gcaacaaaag actggtgttt tagaaacctt ccagatgaac tgctccaaac ccctgtggat
     4201 ccggtaatta tgatgtcttt agcggtgtaa aacgatcttt cttgattgct gaacatgcgg
     4261 gatctgaaga aagccggcag ggtttgatcc ggttacccct agaaaaccct cgcgcccagg
     4321 ggcaccggga cccttcccgg ctccagcaga gagagccctc cgtcctcgtg agatactccc
     4381 agaaccagga cctccgagag aaacccggca atgttctttg gttcaaaatt gaccacgcag
     4441 accaccagtt ttcccaacat ctcttccatg gagtagtcgt ttttcgcctg aagactggat
     4501 cccctgatcc cgatatcctc tccgaagtct atcttcaccc tgtatgaggg ttttcttgcc
     4561 cggggaaaat cctggacatc tatcaccctc ccaaccctga tatccacttt ttcgaaatca
     4621 tcaaaagcta tcatcgatga tgtttctcct ttcatggcca tttttcccat attatcaccg
     4681 aagccgacag aaagagcctc gccacatttt gtcacaggcc gaagaaaggg ctattatgtg
     4741 gcggcgaaac ccggcgcaaa aaaccggcaa agcaggaggc cctgaaaatg gcaaacatca
     4801 tccttatgat agtatgtttt tctctcggga tcgccctgag aaaatgcggc cgtcttcccc
     4861 aggcgactcc gtcggtgctg aactcctaca ttatccacgt ggctctgcct gccctggcgc
     4921 tgcttcacat tcatggactt gagttctctg ccgcgcttct ctttcccgcg gcaatggcgt
     4981 ggctcctttt cgcggggggg tatgtattct tccgtatcct tggaaagacg gcaggcttct
     5041 ccgccggcac aatgggggcg ctgtttctca caggcggact gggaaacact tccttcgtgg
     5101 gactgcccat gatagaggct ttctacggta gggattacct ggggatagga atactggccg
     5161 atcagctggg atcgttcctg gtcctttcga ccatcgggat agcgctggcc gcggcatatt
     5221 cgtcgggcaa gacgtcgtgg ggaggcattg cccgcaaggt gctgcttttc ccccctttcg
     5281 tggctatggt gctggccttt gccctgatta ctgtaccatt tccgctctgg cttcagagtg
     5341 tgctggagag gattggcggg accctgcccc ccctggcgct actttctgtc ggcttccagt
     5401 tgcgggtcgg ccatatccgt gaccgcataa aacccctggc tgcagggctt ctttataaac
     5461 tgatccttgg tccagccctt gtggcagccg tttttatcct gttacttggc gcgcggggaa
     5521 cggagatcca ggtgacggtc ttcgaggcgg caatggctcc aatgataacg gggagcatag
     5581 tcgcggcgga gcatgacctg gacccttcgc tgtcagccct gatggtagga gtcgggatac
     5641 ccctctcttt tgccaccctg ccgctctggt ggtgggtcct cggggcgttc taggacaata
     5701 ctgcttccgg tgctttctgc tcctcgtgga gaaactcgaa gcgcatatta tccgctccga
     5761 aagccctgcc cagggactgc aatgtctgcc tggcagactg ttccgcgtcg gccccgagcc
     5821 cggcgatcat tgtcgccgcc tgggactcgg cagcccgctt ggcggcgtcc ttgagccggt
     5881 tcttgtcctg ctcgctgaac ccgtctccga aaagggtgtt gagaagatcg gggagcaacc
     5941 atggtacaag cctgctcttc tgctcgtcgt agaactggat atcccgaatg ctggtctcat
     6001 aaatacaggg aggcatcctc aaaagatact cccctccagg ttcctcgata atctggaagt
     6061 cctcgctgcg cagatcgtag cgaaaatcta ttacaaactc gaagatcatg gccattttct
     6121 ttttcgtgag tatccaggag aggtatttct tgccgatctc tccccaggtg tgatcgacct
     6181 ccgtaacgat ctccttcgtc aggaccttga agaccgatag ctggccaatc gccctcagtt
     6241 gctggatgct tgaatatatc gtcgtctcgc gacccttggc gccacccgac catttcctgc
     6301 gtatggaccg gacgagcagc acaagagcaa aagcaataac aaaaccggcc agcatggaga
     6361 caagagtgtt cattcattca cacctcccgg agaggtttca aaagaaccat acactgtaat
     6421 cataaaccaa aagggccgga tcccgggcgc gacctcagga aacggacctc ttttgccttt
     6481 ctgctctgtt atttgctcta tcggtcaccc aaaaccggaa ccatacttaa aattatttcg
     6541 cctcaacaag aaacagagcg tcggggagat tccagcctct acctttcctg atggctcttg
     6601 tacgggtaac gctggactaa accccgtttc gtgaagtatg ctgaccggag aatacgcaat
     6661 aactcgtcat aacaactgcc gggagagagg gaaaaaatga aaaaacataa agcgatcagc
     6721 ctgttcataa tgttcattgt tctcatttgc ctgcctgccc tggcggaggc cgaccccgtg
     6781 atcgaagccc cggctttaaa actggggacc ttcaatctcc gcagagcccc cgcgctggcc
     6841 taccaggacg agaagatctc cgccaccctt cagatcaccc tggacaggct cttcctcgat
     6901 cttcggaatt tgacgcaggg gatagtcatt gtcgaccggg acaagttttt ctttaccgat
     6961 gccgaatcaa gggtcagccg gatgacccac gacgggacac agtatattca ccgggagagt
     7021 ccccagggac agataattct cccccccgga ggaagtgtca gaaccggcat ggtccctctg
     7081 gacaatatct ggtggaacga ccagtctaag aaatacgaaa cgatcacgat caaggatgaa
     7141 gatggcaaag aaaagaccat aaagataccc aaagagaggg acaaaaacga caacaggaac
     7201 tgggagatca gggaacttgc gccctggcct tcggggggaa gattgttcag ctccgaggaa
     7261 aagtacatga agctttattc ggggaagcgt ctctccctcc tggtgaccct actcttcaac
     7321 ggccatgaca caaggtacta tgttttcaac ttcgacttcc ccgtctacgt gacagcggag
     7381 gaaagggaaa cggcaaagac aagatcagcg tccctggacc aatacctgat cctgggtgag
     7441 aaaacaaaag ccgggttcat agtgattcag ggtgtcgcca gcggaagccc ggccgagaaa
     7501 aagggcataa aggccggaga ccttcttatg gagatcgacg gaatatccgt gaccggcatg
     7561 gaagtcgatt cggtgaggga gtacttctcg aaaaagactg gggaaaaccg gagcatcatc
     7621 gttgtgacag gaagaaaggg gataaagaat atcgcggtcc tgaaataacc ttcgagctta
     7681 ccgcggcatt agtcctctta ccgggaaagg ctatgttgaa tggagttcca ggggatgatt
     7741 ccccatccct gagcaagacc cgcaatgtgc gcatgtactg ccttctttaa tggctgtatt
     7801 ggttcgttaa ggcactggaa gccgcgaagg aaaagaacga agcctttcct cctgcaatta
     7861 ttacccgaaa aacctcggat aaacacgatt atcaaaagtg taggtgcgac ccttatgtgc
     7921 cctgtttgaa tatttcatat atgatataat tattcgtgac tgcgaaggag atttttctca
     7981 caatgcctcg ggaaatcatt ttctacacaa aaccggatgg aacaagaccc gttgaggatt
     8041 tcctgacatc actccaaccg gaacatctgg ccaaggcaat gcgggtaata gaccttctcg
     8101 aacaatttgg agccgcgttg caaaggccgc acgttgcggt agtccgattc aaaaaatatg
     8161 ccgggctatg ggagcttcga attcaatctg ctggtaacgc ttctcgaatc ctgtattttt
     8221 cggttcataa ggatattttc gttctgcttc atggattcat taaaaagacc gacgcgctcc
     8281 cggtaaaaga acttgaaata gcccgaaaac gaaaagagaa ctatctggag aggtgttgaa
     8341 aatgagcata aaaccaagga cctggaaaga ggttaagaaa gacctttttc gagatcccga
     8401 aactgcttcg gcttatgacg aactccagga ggaattctcc tttatcgcgt cgatgatcga
     8461 ggcgcgccag aaaaaaggac tctcccaggc cgatcttgcc gccagaatag gaacaaccca
     8521 gggcaacatc agcaggcttg aatcaggcag acataatcca tcaatgagat tcctccggaa
     8581 agtggcgcac gctctggaga aggagctgga agtgagcttc aagtaagcaa tctctccaga
     8641 agcaaggggt tgctctccac ccatcgttga ccgctttgtg gaatttcgtc aaaagtgtag
     8701 gtgcgacccc tataaggttc ccctataagg ttcctttctg agtatttgtt gatttgtagc
     8761 cctggtagtg ctatcttttc ctaaagtaag ggaaagaggt gtttttgatg atttccgcaa
     8821 ctaaaaaatc aggaacaact gtcgatcgca aaacgacgac catgcgcgtt agagtccgtc
     8881 cggcactgaa ggaagaaacg caggaagtat tccagaatct ggggcttacg acttccgaag
     8941 ccttcaacct gttccttgaa caggttcgcc tccgcggggg gctccccttc ccgcttgaaa
     9001 taccaaacca tagaaccgcc caggcgatga aggacattcg agcgggacgc agcctgactg
     9061 ccgctgataa tgttgacgat ctttgggata agctcgacag ggaataaaaa cctcgaatgc
     9121 caaaaccatt cttttctgct cagttcaaac gtgacagaaa acggatgctg agacagggca
     9181 aggatgattc cagactcaag aggatagtca gatctcttgc cgaagggatc ctccttgagc
     9241 cccgaaacaa tgatcactct ctttctggag actggtgcga tttcagagaa tgccatatcg
     9301 accccgattg gcttctgatc taccggatgg atgacaaaaa cctctacctg gcaaggacag
     9361 gaacccatgc cgatcttttc aaggcctgac aaggacaaat cctttggggg accctcatag
     9421 gggtcccccg tacacttttg ataaaaggaa ctcttcagga cagataatca agaatagacc
     9481 gacagcaggt aaggattgca tcttcctcag aggggaaagg aacgatcctg agggatcctt
     9541 agtcgtttcg tgacgacgta tgatgcattc tttttcatcc ctatagcctt tttacttgcc
     9601 ttttgattaa gaaagattca gcacgataaa caactttaga tcatttctac ctgtcaacat
     9661 gggtcagttt ttatgcttga cattgcaggt cagagtttta tattaactta gggtgagaat
     9721 tgcccaaagt acctttttta ttaaatttaa aaatgccacc tgcaaccttg taatatcatt
     9781 ggcttgtcta aaactaaaac cccaacaggt caggctttat catgttttgt aaaaagtata
     9841 ggtgcgaccc ctatgagatc ctatatgaga tcccctgttt tgtaaaaagt gtaggtgcga
     9901 cccctatgag ccgaccccta tgagcgaccc ccaggaatcc tttaaaccct ttagtcgggt
     9961 tggagcctcc tgtcaaagcg tgatcgcgca aagatgtccc aactcaggat ttgctctttg
    10021 cacagtctcc ccaaaaggat gaccgggctt gtctccattt cttttgcgaa ggcctccacg
    10081 gcatcatcgc tgaagtttcc cctgttcctg aactcctgat actgttttgg ggggataagg
    10141 gtatctgccg caaagctgtt tgcttcttcc tccctgatat cgtcatcgct attgccaaaa
    10201 ttgacgaaaa tatccttttt gctgtgtccc ttgaccatgt gacccaactc gtggaagagg
    10261 gagaaccaga aaacatccca ataaggatac cgaaaactaa gtgaaacaac tgccttgaca
    10321 ggggaaaccc actgagtcgc gccattgacg gaggtgtgac gcaaatgagg cactatggtc
    10381 agggcaactc cacattttct gcatatttcc cgggctctct taaagtgatc agggaaatcg
    10441 gtacaactgc ctttgccaag gttgcgcaaa agtgggattg atcctttcag ggccttcaga
    10501 gaaaacgatt ctgcaggaat ctcttccgca ttcagaaccg atttcctcag ccaggcggag
    10561 agagcgtgaa aagaggtttt tggagtgtcg cacctccgga aactggccac aaatggcata
    10621 tcctccaggc gctccagaga ggcaaccccg aaaaactgcc tgagatttat taccttttct
    10681 tcgtccttgc gcgttggcgg cacccactcc ttctgcgcca tttctgcgta tgggaatttt
    10741 ttggcctgtt caatatctgc ggaaagaccc ttttttgctt tgatccgcgc catgtcctcc
    10801 tggtagttca tttcaagatt gagccagaaa gaggcactta caccgaagac ggattccagc
    10861 ttcagggccg tatcggccga gatcggagct tttcctttga tgatttcgtt gatggtttta
    10921 ggcgccatgc cggtgcgagt cgacaactct tgctgagtca ttcccttgga ttccagaaca
    10981 tccgacaagg tatccccggg agggatagca tagtcaggct tgtattccgt ataagattta
    11041 ttcaccatga taatccacaa cctccaggat cctgatccct gaaatattct ccaagtctac
    11101 gctgccgtcg ggcaagcagg gcgaagagtt ttcgatgggg agcaggataa gccgccagtt
    11161 gccgcctagg ctgaccgcga actgtccctg tcggtcatgg tgcagtgggt gaaaccccgt
    11221 ccccgggatc ttgctgaaat caagaagatt gccggcagct ctaagttcca tcaacctctg
    11281 gccgaccttc cttgcccggt tttgacccca ttgcctttgt cccgttgcct cctcattgca
    11341 ggccctttcc agcttcctcg acctgaatct tatatccatt cacttctcct gtcaatttat
    11401 ctttacccga taggtaaagg aaagtatttc tttcgactat cgacccgttt cctccgcaga
    11461 cgatccagtt ctccctgcaa ccctgagccc ttctccagga agcaaaggta acgatcaacc
    11521 cgatgataat aaccccatat tatgctcaca tcattaagca tgctgccgac ttgaagaaca
    11581 gccactgcaa aaagaaaggg agcgcctccc cgtgcgggaa gtgctccctt tcttgccttg
    11641 ttccttctaa aggagccgga tgccttttac tcttccagcg ggatgcagat atcggtgacc
    11701 ggtttatctg caccttcctc taccaaccca tgcccagcag ccagagcgga taacctccct
    11761 tgccggggag ttccgtatcg tcgcgaaata cgatatccgc attctttatt ttcttgaggc
    11821 gaccgccaat ttcgataagc tccccctcga cgacgaaatc tccgcgcctc tcgtcgcgga
    11881 ccgattcgat ctctcttccg ctttcctgaa aagccaggac ggcaaaagcc tcccgggaaa
    11941 cacccaggcc tccaccgaac aggctataca ttggcggttc tcccagaaac agcttcgccc
    12001 ccttggagta gactcgatta tcgttttcct tgcgggctat tcggatcaaa ccgacggttt
    12061 ccatcacttc gaaaagctgc agaagctttg ccttcccaag tccccactct ttgcagaggc
    12121 gctcgacatt cagcacgggg acatccgatc tggcaagata cccggttgcg gccttcatca
    12181 gacgaagatg gttgtcggtg atctgcggca cgaagaacgg gatgtcggaa aactgcatct
    12241 tgccgatgac attctcaagc ctgtccatgt aacggccttc ggtgaaaaag gggcgaaacc
    12301 ctccctccag gtattcccga aaaaggcctg taacattggt ttcatcaagg tattcatgga
    12361 tcttcccatt atcgggagag agtgggtcga taacccctgg atccttttct cccttcagga
    12421 ccgcgaattc acgcaaggag agtagaggaa ccctggttat cgggaaccgc cgtgaaagat
    12481 ctgctactcc ttcccttagc accagcgagc tgctgtcgct tgcccagatc ttgtgccttg
    12541 gatatgaatc gtacagggcc ttgaggtcac ggctccagtc ttttgcaaaa tggacctcgt
    12601 cgacgacgat gccctgaaaa ccggacatga atgcggcttc accaacatcc cagagaggta
    12661 ttccggcaag cagggggttg tcagcggaaa tgtaaatcat gtcatcactc attgaaaaaa
    12721 gaagaaaggt ggttttccca acaccccttg cgcctgttat cagtgccccg cggctgttga
    12781 ctggatttgc accgtagaac gggcgaaggg gttccggaag actcgacctg ttccgatggg
    12841 atatggtttc aagctgccgg atgacctgct gcatttccat taggcacccc tcacttttcg
    12901 tctatgataa gccgatttta gcacaaaaac ggactacggt agtccgtttt tgtgaatatt
    12961 ggatttgatc catcgtttct cccccaaaca aagggagccc cttcccttgt ggttgggcgc
    13021 tccctttttc ttgtcattct gttctgttct taaggttccg gatgctgagt tactcctcca
    13081 gagggatgca gatgtctgtg atcagttttt cgggcggagt ctcctctgga aagttgcggt
    13141 atatctcgaa agatggagcc gccgccggct cgcaaccgct cccggggagc cattccccgt
    13201 aaaaccaccg gtagcaggcc aggagtccgt cgtgaggccc cgtgtgccgg aggacggcgt
    13261 atttccctcc cttgatggcc tgcttttcga agcctgattc ctgctcgaaa ccctccgcaa
    13321 cggtcatgca ggcgtcgtaa cggatctttt cggcctcggt gatatccggg tcgtcatagc
    13381 ttatgccgat aaagagcgtt ttgggcccta tcgcggcgct gttgcgggaa tttgagcaaa
    13441 gctttcccca ggcctccccg caccccgcgt agggtccggt gtagcggacc gaaacgactg
    13501 tcatgggttc gaatttctct atcctgactt ccataggttt tcctccctgt ctctggatga
    13561 cggcccggag cccggggggg ctcccgatcc ccctttcgca tcgagcctct ttcctgtagc
    13621 ccgaaggggt tcctccgaaa cgcttccgga aagctctcgt gaaggattcg tgggtctcgt
    13681 acccggcgct gaaggcgata tcgggaatgg accgggagga atacacaagt tcgaaagcag
    13741 cccgttccat cctgagccgt agaaggtgct cccccagggt ttcgcccacc atggcgctga
    13801 agatcctgtg aaaatggaac atcgagaacc ccgccgcccg ggccagatca tccatggata
    13861 ttttctcgtc cagatgttcc tggatgagga actgcacgtt ccttatagcc tgttcgtact
    13921 catcgcgcgt tctctttcgc atcggtcgac ctccgcctga tggaactgta ctccatatcc
    13981 tcatggcgcg cttgacacat attgcgggat ttggcaatca ggtgattttg ggcctgccag
    14041 actcgccagg ttcgcgggac caggttcccc tgaccctttc ttcccccagt tgagaaaacc
    14101 ctgtgcttcc ttcggttttt gacacaattt cttacaagat gattattggt ctataattga
    14161 taaagaatcg ttctgtcgag aagaggtaaa gcaatgactc gtgatgatat catcaggaag
    14221 atccgggaac acgaaaagga aattcaggat ctgggtgcgg aaaggctttc tctatttggc
    14281 tctgcagtca gggatgacct cctgccggaa agcgacatag atattctggt gtctttcgtg
    14341 atgcctgctg attacagaag atacatcaac ctgaagtttt tcctggagga tcttcttcag
    14401 agacccgtgg atcttgtcat ggaaaccgca ttgaagcccc ggataaggaa acgggtggaa
    14461 agcgagatga tccgggttgc gtgatccgga acttcttctg gaggatatcc ttgaatgttc
    14521 tgaaaagatc gcccggtaca cgaagggaat cacaattgag aacttcagac aaaacagcat
    14581 gcttcaggat gctgttgtcc ggaacctgga aatcatcgga gaagcagtca ggaagcttcc
    14641 ggaagacttt cttgaaaaac accccgactt gccgtggcgg gaaattgcgg cccttcgcaa
    14701 catcctcgct cacgcctatt tcgggttgga tctggaaata atatggaccc ttgcgataga
    14761 ggaaactccc tcgttaagca accagatcga atctatcatc aaaaacaggt ctttttagaa
    14821 aaaccccgac agaacccccg taaacggata aacctcatca aaggatcaag tggcatcgac
    14881 agatacgccg gccaggagca ctccagtcgc agcgatggag caccttgaat cttttatggc
    14941 gatatgatgt ataatcgggg acataatgaa ggacgttggg agggaccaat atgaaaaccg
    15001 tttccagcag ggacttgaaa aacaacccgg ggattcttgc ggattctgat gttaccgtcg
    15061 tttattcaag atcaaaaccg atggcggtct gcgtatccgt atcagaaggg gaggatgtac
    15121 tttcacttgc cgatgccatg ctgcagatga gagcgcaaag ggcgctggag cttgcaagaa
    15181 taatggcgag ggagaacgga accgacagga tcccggacag cgagatcgac gccgagatcc
    15241 ggtcactgcg ggctgcgcaa aagaaagact gagcatcccc gtgatctggt ttgtcatgga
    15301 taccaatgtt cttgtttcgg gttttctgcg ttccaacact cctcccggca ttcttctcaa
    15361 cgggctggta aatcgatctt ttggcctcgt gcttgattcc aggatcttcc ttgagtacag
    15421 ggacgttctt gccaggccga aattctgtct gcctcccgct gttacccagc cggtccttgc
    15481 tttgataaat gccaaaggac attggatatc tcccacccga tacggcggga ctctccccga
    15541 cgagggagac cgctccttcg tagaggccgc cctctctgcc ggagtaccca taatcaccgg
    15601 caacctgaag catttttctt ctgttccatt gctgtctgta ctctctccgg cccaggcact
    15661 ggaggctatg agagaacata aacgagggga ataaacaatc gttccgtgcc ctttgccctg
    15721 ggagcggaga tccgggcttt cgtgagtcgt gaatcgtgaa tcgtgaatcg tgaatcgaaa
    15781 aaccaaattc aaaggcgtaa acggtgaacg gtcaacggta aacggtaaac gggaagggca
    15841 gaagggcctg tcattctgag gaccagcggg acgaagaatc cgggtcagga acagatcctt
    15901 cgatcgctac gctcctcagg atgacaaaaa actaaaggcg taaacggtca acgggatggg
    15961 ttctgatgga attgaaccag attcccgccg gtaggcaaag ggcgcggaac gtccgcctgc
    16021 tggcggattc cgcttcgctt cgctcagaat gacaatccct tgctttttcg aatcacgaat
    16081 cacgattcac ggctcacggg tcttgcccgt tcaccgttca ccgtttaccg ctttttactt
    16141 ctttaccctc ctgagcatat cctccgtcat gccgtcgagg tcgtactcgg gggcaaatcc
    16201 ccactctttc ttcgcggccg tgcagtccag ggagttgggc cacgattcgg ctatcccctg
    16261 cctgacggga tccacatcgt aagagagctc gaacgatggc atgaccttcc ttatggacgc
    16321 ggccagctgt tccgggtcga aactcatggc cgagatgttg taggcgttgc gattgaccag
    16381 gcgcgaaggg tcggcctcca tgagctccac gacggcgttg agcgcatccg gcatgtacat
    16441 catatccatc tgggttccgg gcgcgatgta gctggtgtat tttcccttcg acacggcctc
    16501 gtagtagatg tggacggcgt agtcggtggt gccgccgccg gggagcgcct cgtaggagat
    16561 cagcccgggg aagcgaagcc cccgggtgtc cagaccgaat ttcaggtgat agtagtcgca
    16621 cagaagttcc cctgtcacct tggccacacc gtagatcgtg gtcgggcgct gtatcgtgac
    16681 ctgcggtgta tcatcggccg gtgtcgttgg cccgaaagcc gcgatagagc tggggaagaa
    16741 gaacgccgcg ccattctcgc gcgcacattc gagagccgtg aagcaccctc ccgcgttgat
    16801 gtcccacgat ccctgcgggt cggcctcgcc cttggccgaa agtatcccgg cgagatgaat
    16861 aaccgtatcg gctttgaagg agcggatcag ctcagagaac cttcttccgt cgcgaacttc
    16921 cagaagttcg aaagggcctc cctccgaaac cgggcccgca accttgcgac gggccgtggc
    16981 gaggacgttc tcactaccat atatttttcg caggtggggc gtcagctcca cccctatctg
    17041 gccgttggag ccggtgacga tgactctctt catgatatga cccccatctc cctgccgacc
    17101 ctggcaaatc gttctacggc ctgcgaaagg tcctcacctg aatgggcggc tgagaccatg
    17161 cagcgtatcc tggctgtgcc tttgggtaca gtggggaaca ctattgccgt ggcgaaaact
    17221 ccctcttcga agagcttcgc gctgaaggtc ttggcgtcct gggcctctcc gatgatgacg
    17281 ggcgtgatgg gtgtctcgct gttgcctgta ttgaacccta tggcagagag ttccttcttg
    17341 aggaaattgc cgttttccca cagccttgtc acaagttcgt cgcttgactc caggatatcg
    17401 acggctgcct ggcaggcggc aacatccggc accgtgagag cgctgctgaa gaggttaggg
    17461 cgggccttct ggcggaggta gtcgatgaga accttgctcc cggcgacgac gcctcccata
    17521 acgccaaagg ccttggacat ggtgccgacc tctacatcca ccttgccgtg gaggccgaag
    17581 tggtcgacta ttccgcggcc accgcgtcca agcacacctt caccgtgggc atcgtccacg
    17641 acgacgatta cgccgtattt ctcgcagatc tcgacgatct caggcaattt ggctatgtct
    17701 ccgtccatgg agaagacgcc gtcggtcacc agcagttgct tgcccggggc tcccttgtac
    17761 tcctctagtt tctccctgag gctgtccatg tcggagtgct cgtaacggac gaccttcgct
    17821 tttgagagcc tgcaggcgtc tatgatggac gcatggttga gggagtcgct gtagatgatg
    17881 tcctcggctg aagggaccag cgtcgggatg acggcaagat tggcgcagaa gccggactgc
    17941 aggacaatag aggcctcggc gcccttgaac tccgcaagcc ttttttcaag ctccaggtga
    18001 atgctcatcg ttccggcgat ggtccttact gcgccgggac ctactccgta gttgtcgata
    18061 tactcttttg ccttgctcac aagcctgggg tcgttgcaaa gtccaaggta gttattggag
    18121 cagaggttaa ggaatttttt accctcgatc ttcacccagg ctccctgggg gctttcaatg
    18181 gtgcggatgg tcccgtagag gccggcgctt ttcatcgcat caagatcttc ggtgagaaac
    18241 ttcattggga cagccattat cgatcactcc ctgaatcgga tttgtgatag tctgaataca
    18301 gtatcctttt gagcctgttc cggtgtcaac gaagtatagt tttgtgaggt tttcatatat
    18361 tttggcattg gataatatat tttttagggg aacaactcgg ctccagggag gttgaagcgc
    18421 tatggacgga gcaaaggaac tggagaaact cgccgtgcgg atcagcggtg acataagcgc
    18481 ccctcaccac atagccaatg ttctgcggga gggcatatac cgcggcattc tcgaagaggg
    18541 caagcctctg caccagtccc agatagccgc caggcttggt gtcagcccga tccccctgag
    18601 ggaggcgctg cgccttcttg aaacggaggg cctggtcgaa ttccgggggt acaggggggc
    18661 tttcgtcacc gaactttctc tcgaagaagc ccgcgaactc tacgagatgg tggctgcact
    18721 ggaaaccagc cttatgagga cagccttccc cgggataacc cggcggacga tagaagaggc
    18781 ggaacaggtt cttgaaaaaa tggaggccgt ggacgactgc atccgctggc gggacctcaa
    18841 ctttcttttc catacactcc tgtacgaacc cgccgacaga ccgctgaccc tggacatggt
    18901 ggccaggctt cgccagaaga cagaccggac gatacgcacc cacctggact ccatgaggaa
    18961 ggagtcggag ctccagcacc ggaatatcct cgagggggtc gcggcagggg accttgaaag
    19021 atccgtctcg gccctggccc accacctggc ctacacctcc agcgaccttc agtcatggat
    19081 gagaagcgaa caggccggaa ggaagagggt atgaacgagg aagttgagac tggccggacg
    19141 aaagctttct atatccggaa gatcatagaa gaccatcttg aagacatgga agattacttc
    19201 ctggcggctc aaccccgcgg atggggattt atttgcatac ataaggttgt attcaggacc
    19261 taccccaatg ggtcggtcct caaggggacg attaactgcg tttttccagg aaacgcgtgg
    19321 ccgctgtttt ccggcctctc tgtttcctga gtatcgagac gatctcttct gtagtgacct
    19381 gcgcatcgat ccacggaacg tccagagggg aagcagcggc aggttccggc acaagggcaa
    19441 aagtccgacc gtccctcctg cgaataagga ctttgccgga tttttcggcc ttgtcgagaa
    19501 cactcgatag tttttgcctg gcttcagaat aagtaaaaac ctgcatgatc taaaccccca
    19561 ttacctggat ccctatttga gaagctgcac gctgaagcga tcgatccaaa gtcagcaacg
    19621 gcaccgcgtg ccgcgcggca caatccagaa agtaggcatc gtaggcgtaa agtctggccc
    19681 catgggctat agaaacggcg ttcgccatat ccaccgggag atatcgaatg ggtatggtct
    19741 ggaatatctc aagcccccga catgcatctt caagactgag ccttcgttgt ttcagcatcg
    19801 acgagaaagc atttcctatc tcccacggaa tagaacccgg tcccaaaagg gtgtgtcccg
    19861 tagtcatctc aattatgctt tccctttcgg gttcgttcat tatgactgcg aggattgccg
    19921 aagcatcaat tacaatgtcc aaaacaaccc ttctttcttt ttagacaatt gtacaataaa
    19981 catgcaagag agacaaccaa acaccctcgg ctctgcttac ggtctttttt attcattttg
    20041 cagaccctgg accaccaatt accagaatcg gatcccgcag aaaatgctta accgcaaaat
    20101 tgcccttttc ttttgggctg agaggtgcct gcttaaagct tgttgatttg tagccctatt
    20161 agtgctatct tttcttaaag taaggaaaag aggtgcttcg gatgatttcc gcaactaaaa
    20221 gatcaggaac aaccaatgac ctcaaaacga cgaccatgcg cgttagagtc cgtccggcgt
    20281 tgaaggaaga aaccgaggaa gtattccaga acctggggct tacgacttcc gaagccttca
    20341 acctgttcct tgaacaggtc cggctccacg ggggactccc cttccctctt gaaataccca
    20401 accatagaac cgcccaggca atgaaggaca ttcgcgcggg aagaaccctg acggccgctg
    20461 aggatgttga cgatctttgg gataagctcg acaaggaata aaaatcccga atgccaaaac
    20521 cattcttttc ggcccagttc aaacgtgaca ggaaacggat gctgagacag ggcaaggatg
    20581 attccagact taaggaagta gtcactgctc ttgccgaagg gatcccactt gaacctcgaa
    20641 acaatgatca caccctgtct ggagaatggt ccgatttcag agaatgccac atcgaccccg
    20701 attggcttct gatctaccga atggatggcg gaaacctcta cctggcaagg acaggaactc
    20761 attccgatct tttcaagtcc tgacaagggc ttgtttcgca tggtgcagaa agaacgggag
    20821 gaaagggtga taaaaaatgc ctgttcccac ttccgtccgc ttgagcgaag agaccgaaaa
    20881 acgtctttcc cgtctcgcgc ttgagactgg acggacgaaa gctttttata tccggaagat
    20941 catcgaagac catcttgaag acatggaaga ttactatctg gcggctgaaa ctcttgcaaa
    21001 ggttcgcagg ggggaagaaa aggtctctgg ctgggatgaa gtcgtgagcg accttaaaaa
    21061 ataaccggct tcgacaaacc ccactgtcgg gcacagcgac cgttttatcc ggtggatgta
    21121 ggcatacggt caagatcaag gtttgctccc aagggttttc ccctccgagg gattctctgg
    21181 agctcgattc gcccccgaga aggggacttt gcctgaaacc gttcggggtt atccctgctc
    21241 cggggcgacc gcgcaacagt ccctcaccgg ctcatatgtc tcctgcgacc ccccggaagg
    21301 ggttcctgcc ccctattgta tgcgtaaaat aagaatcaag cccgaaatgg gttgcccggg
    21361 accgggtcct gctgtctact cgatcctctt cgcctttttg tagacatctc cgtcgtttct
    21421 tttgccgata accgacacat aaagaaactc atcgtcaaaa cgataaacga tacggtactc
    21481 tcccgaatcg acacgaaaga aagggctccc cttcatgctt atggagtccg ccggacgtgg
    21541 ttcctcaaga agcatcatta ttttccgtgc gagttgcgcc gaatgctttt tcggaacacg
    21601 ggaaaggaaa ctgtcggcct ttttggtgat gaggagccgt ctaatcatcg gaggatgaaa
    21661 tcaggcccgt cagtcttttc atggaccggg cttcgtccag gaaacccgaa gactccgcat
    21721 cggtcgccct ggcgatccag taactttcct cgatccccag gagcctctcg tattcgcgat
    21781 gagataataa gacagcagaa accctcccgg agcgtttgat aatcaccggt tcgacaaggg
    21841 attcctcaag tatcctgccg aagttctgct tggcctcggt tgcgcttgcc gttttcattc
    21901 cgaaacctcc gttccttttg actgtagggc acattatata ccaaacagac aaaatggaca
    21961 aaaaggccga aatagacaat caacaggaaa atggagcaga tgcagaccct ctttactaat
    22021 tctggagcag ggccttgagt tcggcgatct ccgccctcag atcttcgatc tgtttttcct
    22081 cgtatgattt gccgagtttc agggtaagtt caactccgac ccggtccagt ttggagcggt
    22141 tggcggagta gttcgcgaag acgttcca
//
LOCUS       DAOTIQ010000028        22116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2797_length_22116_cov_7.276279, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000028 DAOTIQ010000000
VERSION     DAOTIQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 22116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22116
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_22116_cov_7.276279"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="P5541_05115"
     CDS             <1..664
                     /locus_tag="P5541_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRV71394.1"
                     /translation="DGLMRATNVVIAGKVCVIVGYGWCGRGVAMRAKGLGARVVVVEV
                     DPHRAYEALMDGCEVMDMKGAAAEGDIFLTLTGNYGVIRKEHFAHMKDGAIMGNAGHF
                     DVEIDKKGLESLSKEIMDVRDNVRTYLMKDGRRLNLVADGRLVNLAAGDGHPIEIMDL
                     SFALQLESAIHIRKNGGKPGVYNVPREIDEAVIRTGLAAQGVVLEVLTPEQEKYLLEW
                     RE"
     gene            701..1996
                     /locus_tag="P5541_05120"
     CDS             701..1996
                     /locus_tag="P5541_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV71395.1"
                     /translation="MDTLFRDTIFLDTENGSASRGDVMVRDGVISSTGRFPGNVPEDC
                     EVFEGRGSVLVMPGFVNSHTHAAMVLLRGFGEELPLREWLEKRIWPVEAGLTPEHVYW
                     GSRGAILEMASTGTTCFADMYFEMDEVARAADESGMRCSLCRGIVGDDEKKILEGIDL
                     YRKWDRKGLTRVFLGPHAPYTVPMETMRRLTAEGNSLGTGVHTHFLETEWEISYIRET
                     FGLSPFSYLESSGLLGTPKTILAHGVWIEKEDLPLLEKSSVTIAHNPASNMKLGSGFA
                     PLPSMLSSGVCVSLGTDGAASNNRLDMWGEMRLCTLIHKGQSKDPTVVTAKEVLRMAT
                     LEGARAAGFDDVGLIREGWQADLVVVDLDSPQFQGWDMENIAGFLVYAGSSRDIIGTM
                     VAGKWLYRRDRPDHEGQEETMKQMARCRIELARAAGIVA"
     gene            2034..4682
                     /gene="alaS"
                     /locus_tag="P5541_05125"
     CDS             2034..4682
                     /gene="alaS"
                     /locus_tag="P5541_05125"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRV71396.1"
                     /translation="MQWRSGKELRETFLSFFEENGHRRFPSFPLIPDDPTLLFTIAGM
                     VPFKPYFMGKRKPDFNRATTAQKCVRTNDIDNVGRTARHHTLFEMLGNFSFGDYFKSR
                     AASFAWEFLTEKVGLDPDRLYPTIFRDDEEAFVIWNSEIGVPSERIHRMGEEDNFWSA
                     GPVGPCGPCSELIYDQGPSFSCGRPDCGVGCECDRYLEVWNLVFMQYNRLEDGTLEPL
                     PRQNIDTGMGLERLSSVVQQVAGDFETDLFRPLVDRSCEICGIRYGADKAGDMAARVI
                     SDHFRATAFMIADGILPSNEGRGYVLRRILRRAVRFGRLCGVRKPFLMDLYPVLMDVM
                     GDPYRELGDQRALIEQVVGLEEARFGRTLEQGLRLLETEIDSMRKPGGNVLSGEVAFQ
                     LYDTFGFPLELTEEVCQESGISVDRDGFSVEMEKQKERARAGARVTAGSFQGTVFDRI
                     REDYGPTDFRGYVSETIETEVIAVVRDGERSERADEGEEVLLVLSETPFYGEGGGQSG
                     DTGTIKGEGFNAKVLDTHKFAGDLVVHRSRIITGVVEERAKVLACVESIRRRSIKAHH
                     TATHLLHEALSRVLGDHVAQAGSLVTDEFLRFDFTHIKPVSRDEINRVEDIVNQQVLM
                     NTPLDVMETDMESARTMGARALFDEKYGERVRVVSVPGFSTELCGGTHVDATGDIGPF
                     RIIREEGIGAGVRRITALAGVPCLGYFRKISENLSGLESILSVPADLVGQKVSELLEE
                     NRSLNSEAVKLRTRVALSRVDELLEEKVQAGPVSVVTARYDGIEPDTARSVGDSIRKR
                     AEGVVVVIFTVSGESTQIIAMADKVAVSRGADCGKIVKKISGDLGGGGGGRPDMAQAG
                     VKNTSGIDMAIKKTPETVRSILAEKN"
     gene            4679..5098
                     /gene="ruvX"
                     /locus_tag="P5541_05130"
     CDS             4679..5098
                     /gene="ruvX"
                     /locus_tag="P5541_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRV71397.1"
                     /translation="MKRIIGLDIGKVRIGVAISDPLGTFAQGIAVLDAGKDWIGLLRK
                     TIEERNVGTLVIGLPVRTDGSLGPEAVWVDSVIKRLRAEFGELEITSWDERFTTTSAT
                     QSLLEGDVSRKGRKKVVDKVAATIMLQGYLDNRRRGT"
     gene            5095..>7524
                     /gene="mutS"
                     /locus_tag="P5541_05135"
     CDS             5095..>7524
                     /gene="mutS"
                     /locus_tag="P5541_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRV71398.1"
                     /translation="MNLPEEIKMTPMLEQYFEWKKRYPDCLLFFRMGDFYEMFFDDAS
                     IASSILDIALTARDPARKIPMAGVPFHSVESYLAKLVSSGRKVAICEQVSEPDGRNLV
                     ERQVVRVVTPGTFLPGESSAEGRIAAVAPQGNDISLALLNCASGVISAGTLSRETAKA
                     ELAGFCPKEIIAPQGFDEQARGLMPFGREYVFTPRRKEEFNPVDASRMICRRMGVSTL
                     AGFGIEDLDQACGCAGVLYTYIEETQFRSPEGVLAFSPLQELSSMRIDPSSIENLEIV
                     GEEGASLVGTLDRCRTPGGKRKLREWLLHPLLDPAEISRRQDRVSALVEASGSRAAIT
                     SSLSGCGDLDRAISRFAFGSGGPRDALTIRENLRILPRLKAALETAGIQDLLELDEGV
                     GSLFDLLDKSLKDELPRIASGGELIREGFDEDIDSLRKMKKGESQWLVNFVEELKKET
                     GIKNLKTGYNKVFGYYIEVPRSSASMVPDSFVRKQTLVSGERYVTEELKSFEERMISA
                     SDELVRREEKVFRDVCRFIVEMSSRLRCVASAMAAIDVFAAFAQISLMNRYCRPFVDH
                     GDSIFIEGARHPVVESRMAGDVFTPNDISLDSGESRIAIVTGPNMAGKSTYLRTAALL
                     VIMAQAGCFIPARSARLGVVEKVFSRIGARDEIARGRSTFMVEMIETANILNNVTAKS
                     LVVLDEVGRGTSTYDGMSIAWAVLEYLQQHAGKRPKVLFATHFHELTALSETLPGVTN
                     LSMAVEERDNGVIFLYKVVPVPADRSYGIEVARLAGIPEVVIERSRKILEGFEKRKAA
                     SMENGAEEGAEE"
     assembly_gap    7525..7624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7789..9480
                     /gene="mutL"
                     /locus_tag="P5541_05140"
     CDS             7789..9480
                     /gene="mutL"
                     /locus_tag="P5541_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRV71399.1"
                     /translation="MRPIRHLPADVSSRIAAGEVIENPASVVKEMLENSIDAEASRIS
                     VILSNGGKSLISVMDDGTGIPGEEMPLAVERFATSKISTLEDLSGILSFGFRGEALAS
                     VCAVSRMEMHSCRKGLKKGAMIRCEGGEINLHVQVDAMEGTRILVRDLFFNLPARRKF
                     LKGSAAELRKVASVVRDYSSAFPEITFVLESEGRKIWSTEGDGERVRVLGRMWGSEDV
                     RSGSETAGALEIEAYWSPVPGSRRKDVIIFVNGRRVREPAILAALSSCGEGVAGNWFF
                     SIKAPGEMVDVNVHPGKAEVRIHPSIPLFETTRAVVGSLLGGFSVGNKSIETSSGTET
                     KTAFRQGEWNDLRSLDHDGSFFSRVAEPPAYAFGETSVAAGRKGYSFIGQISSGYLLF
                     ESEGDLVLVDPHAAHERINYEKFLKARSEAVPQSIPFPEQVPPSLSLRAAEIRDSLES
                     AGFRFEERDGTLFIKALPEGCGSVSSKDPVEALRVWAGLIEKPGCFPDGKVPMASEAC
                     MASVKLGERIAIEEALKLISDLLECQEPFACPHGRPVMARLGSSDLARLFGRESK"
     gene            9477..10424
                     /gene="miaA"
                     /locus_tag="P5541_05145"
     CDS             9477..10424
                     /gene="miaA"
                     /locus_tag="P5541_05145"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRV71400.1"
                     /translation="MKIPENSELLCVIGPTASGKTALSLELARSLDGEIISVDSRQVF
                     RFMDVGTDKVSMNTRAEIPHHMIDVVDPDGYFSAADFAEGAKRIVVDIVSRGKTPILV
                     GGTPFYYRALFSGILSAELKSWPEVRKEYEARWRDGEAKFLYQRLEREDPDYASHVHP
                     NDAFRVIRALAIIDKTGKKPTYWRKRALKRKTGLSPFYIGLYPGREALYSNIEKRVRE
                     QFLKGYPEEVENLLARGYGEDLPSMRGFGYRELAAFHGGRMSLKEALEGDIRSTKAFA
                     RRQMTWFRRFSPSLWYYVPGSGITEQVRKVIGLWRTRHS"
     gene            10403..11305
                     /gene="ispH"
                     /locus_tag="P5541_05150"
     CDS             10403..11305
                     /gene="ispH"
                     /locus_tag="P5541_05150"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRV71401.1"
                     /translation="MENKTFVIPRNTELVFAEPRGFCFGVRRAISTLEEALAKFGRVY
                     ALGSPIHNSREIERLRSMGLEIVEDPSEIPQGGVVFIRAHGVSDRVVEHLTQKEANII
                     DGTCPFVRKAQERATFLAEEGYHVIILGDKEHPEVRAIREFARGPVTVVDPFDILPAG
                     NTGASRIGIVSQTTQRRESLSSLVSFVCARTPEVRVFNTICGATASRQDAVATLADKV
                     DGIIVTGGKNSANTAKLVEICENAGTPVLWIEDEGEMESGWFRDKRTIGIAAGASTPD
                     WLIHRLETKLASAKSAREDEWEDD"
     gene            11298..12863
                     /locus_tag="P5541_05155"
     CDS             11298..12863
                     /locus_tag="P5541_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV71402.1"
                     /translation="MTEEKRNQLEEEHVSSGTIEPTEITESTESNEVNDPAETMESFL
                     EQYEVGQIHRGKTVEGTVVEAAEEGWLIDVGFKCEGFLPKHEWSHRVLVESKETPSVG
                     DTVRVQVVSIKQGEESQLLVSRWRTEFDERWEAMEESLSKDEFIEVRGLRKVKGGLIV
                     DCCGIEGFIPVSHLAEEGRGVNPSRLVEDSFSAKVIERDRKKRRVVLSRRAILESEMN
                     EQRESFYKQAEIGQIVEGTVSSITSFGAFVNIGPVDGLVHNTELSWGRNSKPREIVNK
                     GDRVNVKIIGIEPENNRISLSIKQTQPDPWEEASSRFKEGDRVTGRVTNLTEFGAFVE
                     IEPGIEGLVHIGDISWQRIRHPKEALRRNQQIEVCILGIDEAGKRISLGFKQLNDPWR
                     DIDEKFQVNGDYTVKVVRLTDFGAFVELEEGIEGLIHVSQLSRKRVEKPGDILGEGQE
                     VLARIIEIKPDERRIRLSMSALEEPEPKREREVREPDKRRKKSNVKSDSSQFMDGETN
                     VTIGDLWQEALKD"
     gene            12932..13414
                     /gene="def"
                     /locus_tag="P5541_05160"
     CDS             12932..13414
                     /gene="def"
                     /locus_tag="P5541_05160"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRV71403.1"
                     /translation="MSVHRIREYPDPVLMSTAERVGEFNEELLSLVDDMWETMAEYDG
                     VGLAAPQIGFGIQVAVVGWKDRKIILVNPEVLEVEGETVQEEGCLSAPGVFEKVVRPD
                     RIVVQAQDEKGKPQRIEETGFLARAIMHEIDHLHGQLFFDRLSPLKRKFLKRKLQKRG
                     "
     gene            13417..14349
                     /gene="fmt"
                     /locus_tag="P5541_05165"
     CDS             13417..14349
                     /gene="fmt"
                     /locus_tag="P5541_05165"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRV71404.1"
                     /translation="MDLGYPVWFAGSGSFGANCLANVSEEVCISRVITAPPKPAGRKL
                     EKRITPVESRACTLGLPITRTENINTDVIIRDLFEKERPCCVVVVDFVQKIGEPFLST
                     GPPGCLNIHPSLLPAYRGSAPVQRALMNGEVITGVTVFRLVEEMDAGPVMGSVEVEIG
                     PDDTSGDLFEVLSLKGSRLLVDGLKLYSKGRIPFKEQRHEKATFAPRIQKSETKLSWE
                     KPSIYLHNLVRAMNPSPGTYVLINGKRLKIWKTRPHQESGEPGEILDSVPGNPVVACR
                     EGSIELIEVQQEGKNRADGASWIRGTSFRKGDIL"
     gene            14370..15107
                     /locus_tag="P5541_05170"
     CDS             14370..15107
                     /locus_tag="P5541_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71405.1"
                     /translation="MPAGPICGRQWPRAIAVTGALGSGKTECVLNMALSFHECGEKIT
                     IADVDIINPYFCVRQIAENLRRMGFTILTPKESARWSDMPVISPEVGWALKTEGKRLI
                     LDVGGDAVGVRALKQYSEPLEEAGYLLILVVNPFRPQTGDVSGIRNMILNMEKLSGLK
                     IGALAANPHLMDQTTSADLLWGYRQVCDAGKDIGLEVLFGTVPPHLESESDYIGKESP
                     VDLLFMTRHMMLPWEEGYMWTQAGDRS"
     gene            15176..15391
                     /locus_tag="P5541_05175"
     CDS             15176..15391
                     /locus_tag="P5541_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71406.1"
                     /translation="MAKGRINVLAEFCKSCGLCVQACPVKVLRISETINSKGYRPAEQ
                     FKEGCIACGMCATTCPDAAIEVFRIEE"
     gene            15415..16467
                     /gene="vorB"
                     /locus_tag="P5541_05180"
     CDS             15415..16467
                     /gene="vorB"
                     /locus_tag="P5541_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRV71407.1"
                     /translation="MARVLMKGTEAIAEAAIQAGCKYFFGYPITPQNEIPEYMSGKLP
                     KIGGAYVQAESEVAAINMVMGGGSTGTRIMTTSSSPGVSLMSEGISYIAGCEIPAVIV
                     NVMRGGPGLGGILPSQADYFQSTKGGGNGDYRLLVMAPSTLQEAVEVTQEAFDLAQKY
                     RNPVMILADGFMGQMMEAVEIKEHVSKDLPPREPWALGCMGERGSKRNILKSLFLNPE
                     HLEEHNIKLQEKYKLMEKEVRFEQYQCEDADIILAAFGTTARIARSAINNLRKEGVKV
                     GMLRPITLFPFPKDIMAKLAEKAKHVLVVEMNMGQMVEDVQAAICGKAPVAFFGHAGG
                     IAPSVREIEDQIRAII"
     gene            16488..17234
                     /locus_tag="P5541_05185"
     CDS             16488..17234
                     /locus_tag="P5541_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV71408.1"
                     /translation="MKETKIYERPKSWVKEVHTHYCPGCGHGIAHRLLCEVIDELGIQ
                     NDTIGTAPVGCAALMYDYIDVDFCEAAHGRAPALATGIKRTRPDKIVFTYQGDGDLAS
                     IGTAEIIHVANRGEKLTTIFINNAIYGMTGGQMAPTTLLGQKATTCPFGRDPLLAGYP
                     MRMCEMLATLETPGYITRVSVAKPKYIIEAKKAIKKAFQNQIDGKGYSFVEILSNCPT
                     NWGMAPVPAFEWTESTMIPHYPLGVFKEFN"
     gene            17250..17810
                     /locus_tag="P5541_05190"
     CDS             17250..17810
                     /locus_tag="P5541_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV71409.1"
                     /translation="MSGFFRGMIAAGFGGQGIMVLGQLVAYTAINEGRYVTWIPSYGP
                     EMRGGTANCGVAISDEEIASPVVAESDVTVIMNNPSLEKFEKTVRPGGVLAYNSDLVK
                     YDSPRKDVILLPIPAHSVASALGSDRISNIVVLGAIVRATDLISREVCIETIKDKLGS
                     RKPQFLPMNLEAFEKGQEIAEKLLKK"
     gene            17818..18375
                     /locus_tag="P5541_05195"
     CDS             17818..18375
                     /locus_tag="P5541_05195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRV71410.1"
                     /translation="MGSGEEKISGTLVYRGRIINLRVDNVRLQDGNKASREVVEHAPA
                     VGIIAEADDGDLFLVRQFRYPAGEFLLEIPAGIVEPGEKPADTARRELQEETGFDARD
                     IVEISRFYTSPGFSNEMIILYHASGLLPSKMECDPDEFLDLVRMSETELLEAADTGKI
                     RDSKTLSAVYWYLAKRSAISRKRDG"
     gene            18407..19294
                     /locus_tag="P5541_05200"
     CDS             18407..19294
                     /locus_tag="P5541_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRV71411.1"
                     /translation="MEKNDKDLALFFSYLRYERGCSENTIQAYGSDLRAWRKFCGETG
                     TAFCPPERDGLSSFFSTMTLSGKSKSSIQRAAATLRTWLRFLETEGETESDGSLPRLP
                     TREKKLPRILTEGEVNRLMTACEGDDPLSLRDRAIVELGYGCGLRAGEISGLVLRNVG
                     FDSGFLKPGGKGNKERIVPLVGKVRASLEMYISRSRETLGAPATDRVFVSVNGNPLRR
                     EDVWRIIRKRGKEAGIAESRLYPHILRHSFATHLLRRGMDLRTLQELLGHSSIMTTEK
                     YTHFDTELRDVYDKSHPRA"
     gene            19336..20172
                     /locus_tag="P5541_05205"
     CDS             19336..20172
                     /locus_tag="P5541_05205"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRV71412.1"
                     /translation="MTDYREKVREAREYVEKRFKHSPLAVFVLGSGLGAFADSIQEKI
                     EIPYEDIPGWPESTAPGHSGKLVCGFIGQTPIVAMQGRHHFYEGYSMSEVVFPVRVFG
                     EMKVPNYFGTNATGGISHSLSPGDLVLVYDHINFQGHNPLRGPNEDLWGPRFPDMTYA
                     YDRGLLELVEAAASSLNLQIRRGIYAAFPGPSFETPAEIRMMRILGADVVGMSTVPEV
                     ITARHMGMRSCVISCVANYAAGMSEKVLTHEEVLEEMDKASGRLTSLLQAVVLKLGGE
                     IR"
     gene            20169..21485
                     /locus_tag="P5541_05210"
     CDS             20169..21485
                     /locus_tag="P5541_05210"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRV71413.1"
                     /translation="MNPVSFIETKRDGLENSPGDIREFVRLALSGGMKDYQISSWLMA
                     VFFRGMTPSENRTFTEALAGSGKTVRFPDGLFAVDKHSTGGVGDKTTLVVVPLVAACG
                     VPVAKLSGRGLGFTGGTVDKLDSIPGFRSSLDMKNFIDQVEKTGCAVSGHSKDLAPAE
                     GVFYKLRDVTGTVPCIPLITSSIVSKKVAGGSSAFVFDVKCGNGAFMKDLDSAKQLAR
                     QLVDLSMDLERPAIAMITDMSRPLGRWTGNAAEVMEAIDVLRGEGPEDTLELSLRLAG
                     AMIHLGGEVSSIEEGVKIAEKSIFSGKGLEKFLEMVKVQGGDPGDFLDAGKRRNHLAP
                     SVFEIEALEAGWVTECDALLIGEAVRSIGGGRLTREQEIDPSVSCEIVTVRGEKVSKG
                     QPLARLYYRNDGPEIENAVEMVRRAFRTGERPGTEKLFLGFEGPAT"
     gene            21499..22059
                     /locus_tag="P5541_05215"
     CDS             21499..22059
                     /locus_tag="P5541_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV71414.1"
                     /translation="MSGVRNLWEEPSGGLVERSGKGDEEALSEMMLRFTPLIRYIARE
                     FKGSFLAEEDDLLQEGYISLIRAVRGYEANRGKFSSYLFSCVRNGMISYLRKNKNKNR
                     ESPLDPETEASGKGSLCWPVEELLPPDFTSLLTITEMTVLDAFLETGSVSSSAVVLGW
                     QRKKVDNAMQRIRKKLRGWRDARAEA"
BASE COUNT         5665 a         4895 c         6502 g         4954 t
ORIGIN      
        1 cgatggactc atgagagcca ccaacgtcgt tattgcgggc aaggtttgtg tgatagtcgg
       61 ttacgggtgg tgtggccggg gagttgctat gcgagccaaa gggcttggcg caagggtcgt
      121 ggtggtggaa gtcgatccac acagggcata cgaggcgctc atggatggtt gcgaggtaat
      181 ggatatgaaa ggcgcagcgg cggaggggga tatattcctt acgctcacgg gcaactatgg
      241 agtcatcagg aaagagcatt ttgcccatat gaaagatggg gccatcatgg gcaatgcggg
      301 acattttgat gtggagatcg acaagaaggg gcttgagtca ctttcgaagg agatcatgga
      361 tgttcgggac aatgttcgaa cataccttat gaaagatgga cgaagactta acctggttgc
      421 cgacggcagg cttgttaacc ttgccgcggg ggatgggcac cccatagaaa taatggacct
      481 cagttttgct cttcaactcg aatccgcgat acacataaga aaaaacgggg gcaaaccggg
      541 tgtttacaat gtcccccgag agatcgacga ggccgttatc aggactgggc ttgccgccca
      601 gggagtggta ctggaagtac tgacaccgga gcaggaaaaa tatctcctgg agtggaggga
      661 ataacccctc ttttcagggc ccaggaaggt gataaaggtt atggacaccc ttttcaggga
      721 tacgatcttt cttgacacgg agaatggatc ggcttccagg ggagatgtaa tggtccgtga
      781 cggggtcatc tcgtcgaccg ggcgcttccc gggcaatgta cccgaagatt gtgaggtttt
      841 tgagggtcgc ggcagtgtgc tggtcatgcc cggtttcgtc aacagtcata cccatgccgc
      901 aatggtcctt cttcgcggtt tcggtgaaga actgcccctg agagagtggc tggaaaaaag
      961 gatatggccc gttgaagcgg ggctaacgcc ggaacatgta tattggggat ccaggggagc
     1021 aatactggaa atggcgtcga cgggaaccac ctgttttgct gacatgtact ttgagatgga
     1081 cgaggtggcc agggccgccg atgagtctgg aatgcgctgt tccctttgca ggggaattgt
     1141 cggggacgac gagaaaaaga tccttgaagg gattgatctt taccggaaat gggacagaaa
     1201 gggtctcaca agagtctttc tggggcccca tgctccctat acggttccga tggaaacaat
     1261 gagaaggctg acggcagagg gtaattccct tggaacgggg gttcatacac attttctgga
     1321 gacagaatgg gagatatcct atatcaggga aaccttcggc ctttcgccat tttcctacct
     1381 ggaaagttcg ggtcttctcg gtactcccaa aaccatcctt gctcacggag tctggattga
     1441 gaaggaagac ttgccccttc tcgaaaaatc ttccgtaacg atcgcgcaca atcctgccag
     1501 caacatgaaa ctgggcagcg gttttgcccc cctgccgtcg atgctttcat cgggagtctg
     1561 cgtttcactg ggtaccgatg gggcggccag caacaacagg ctggatatgt ggggggagat
     1621 gcggctttgt acgctcatcc acaaggggca aagtaaagat cctacggttg ttaccgcaaa
     1681 ggaagttctc agaatggcta cgctcgaagg agcaagggcc gcaggatttg atgatgtcgg
     1741 tctcatcagg gaaggatggc aggccgatct tgtcgtggta gatcttgata gtcctcaatt
     1801 ccagggatgg gatatggaaa atattgccgg attcctggta tatgccggtt cctccaggga
     1861 cattatcggt accatggtcg ccgggaaatg gctgtatcga agagaccgtc ctgatcatga
     1921 aggacaggaa gaaacgatga agcagatggc gcgatgcagg atagaactgg ccagagcggc
     1981 cggcattgtt gcctgatgat ataatcaccc ttaaaacgaa ggagaaccca ccaatgcaat
     2041 ggagaagcgg aaaagaactg agggagacat tcctctcctt ctttgaagag aacggacaca
     2101 gaagatttcc aagttttccg ttgatacccg atgacccgac gctgcttttc actatagcag
     2161 gtatggtccc tttcaagccc tattttatgg gaaaaaggaa gccggatttc aacagggcta
     2221 ctactgccca gaagtgtgtc cggacaaacg acatagacaa tgtcggaagg accgcgaggc
     2281 atcataccct ttttgagatg cttggaaact tcagtttcgg tgattatttc aagagcaggg
     2341 ctgcttcttt tgcctgggaa ttccttacag aaaaagtagg gctcgatcct gacaggcttt
     2401 acccgacaat tttcagggat gacgaagaag cctttgtgat ctggaactcc gagatcggag
     2461 ttccctccga acgcatacac aggatgggcg aggaagacaa tttctggtcc gccggtcctg
     2521 tcgggccgtg cggtccctgt tccgaactga tctacgacca gggtccctcc ttctcctgtg
     2581 gaagacccga ttgtggagtc ggatgtgaat gcgatcgcta cctggaggtt tggaaccttg
     2641 tattcatgca gtataaccgg cttgaggacg ggactcttga accccttcca aggcagaata
     2701 tcgatacggg catggggctt gagagacttt catcagttgt ccagcaggtg gccggggatt
     2761 ttgaaacgga tcttttccgt cccctggtgg acagatcctg cgagatctgc gggatccggt
     2821 atggcgctga caaggcaggg gacatggcgg ccagggttat ttctgatcat ttccgggcga
     2881 cggcctttat gatcgccgac gggatccttc cctcgaatga gggcaggggt tacgttctca
     2941 gaaggatcct cagaagggcg gtacggtttg gaagactttg cggtgtccgc aaacccttcc
     3001 tgatggacct ctaccccgtc cttatggatg taatgggaga tccctacagg gaacttggtg
     3061 accagcgcgc tcttattgaa caggtagtgg gtctggaaga ggcgcggttc ggcaggaccc
     3121 tggaacaggg actgcgtctt cttgagacgg aaatagactc catgagaaaa ccgggaggca
     3181 atgttctttc gggtgaggtt gccttccaac tgtatgacac tttcggtttt ccccttgaac
     3241 ttacggaaga agtatgccag gaaagcggca tatcagttga ccgcgatggt ttttcggtgg
     3301 agatggaaaa acagaaagaa agggcacggg cgggagcccg ggttactgcc ggaagttttc
     3361 aggggacggt atttgacagg atcagagaag attacgggcc aacggatttc agaggctacg
     3421 tttcggaaac cattgaaaca gaagtcatag cagtggtcag agatggagaa agatcagaaa
     3481 gagccgacga gggtgaagaa gtcctgctgg ttctctctga aacgcccttt tacggtgaag
     3541 gcggtggaca gtcaggtgac acggggacaa taaaaggtga aggctttaac gcgaaagttc
     3601 ttgataccca taagtttgcc ggcgatctgg tcgttcacag atccaggata atcaccgggg
     3661 ttgtagagga aagggcgaaa gtcctggcct gcgtggagtc gataaggaga agaagcatca
     3721 aggctcacca taccgcaaca catcttcttc acgaggctct ttcgagagtg ctgggagatc
     3781 atgtggccca ggccgggtcc cttgttaccg acgaattcct gcgttttgat tttacccata
     3841 tcaagcccgt cagcagggat gagataaaca gggtcgagga tatcgttaac caacaggttc
     3901 ttatgaatac tcccctggac gtgatggaaa ccgatatgga aagtgcgagg accatgggcg
     3961 cgagggccct tttcgatgaa aaatacgggg aaagggtaag ggtcgtttca gttcccggat
     4021 tcagcacgga actatgcggg ggtacccacg ttgatgcgac cggtgacatt ggtcccttca
     4081 ggataatcag ggaagaaggt atcggtgcgg gtgtcagaag aataacagcc ctggcgggcg
     4141 tgccgtgtct cggttatttc cgaaaaattt cagaaaatct ttcgggactg gaatcgatcc
     4201 tttctgttcc ggcggatctg gttggacaga aggtctccga actgctggaa gagaacaggt
     4261 cactgaattc ggaagccgtg aagctgagaa ccagggtagc cctttcaaga gtcgatgaac
     4321 ttttggagga aaaggtccag gccggtcccg tatctgtcgt cacggcgaga tacgatggta
     4381 tcgaaccgga tactgcccga agcgtcgggg acagtatcag gaagagggcc gaaggtgtcg
     4441 tggttgttat ctttaccgtg tcgggggaga gcacccagat cattgccatg gctgacaaag
     4501 tggcagtttc ccgtggagcc gattgcggaa agatagttaa aaagatttcc ggggatcttg
     4561 gtggaggtgg cggaggccgc cccgacatgg cccaggcagg ggttaaaaat acttcaggga
     4621 tcgacatggc aatcaaaaaa actccggaaa cagtccgttc aattctggcg gagaaaaatt
     4681 gaaaagaatt atcgggcttg atatcggaaa ggtcaggata ggagtagcaa taagcgatcc
     4741 cctgggaacg ttcgcccagg ggatcgctgt tcttgatgct ggaaaagact ggatcggtct
     4801 cttgagaaag acgatcgagg aaagaaatgt cgggacgctg gtaataggtt tgccagtcag
     4861 gaccgacgga agtcttggtc ccgaagcggt ctgggtcgat tccgtcatca aaaggctcag
     4921 ggcggaattt ggtgaactgg agataacttc ctgggatgaa agattcacaa caacatcggc
     4981 tacccagagt ctgcttgagg gggacgtttc aagaaaaggc cggaaaaaag ttgtggacaa
     5041 ggtcgccgcg acaataatgc tgcagggata tcttgacaac aggagaagag gaacgtgaat
     5101 ctgcctgaag agataaaaat gactcccatg cttgagcagt actttgagtg gaagaagagg
     5161 tatcccgatt gcctcctttt cttccgcatg ggagattttt acgagatgtt ttttgatgac
     5221 gcaagtattg cctcaagcat tctggatatt gctcttaccg caagggaccc tgccaggaag
     5281 atccccatgg caggggtgcc cttccattcg gtggagtcct atcttgcaaa acttgtttcc
     5341 tctggaagaa aagttgccat ctgtgagcag gtttcggaac ccgacggcag gaacctggtt
     5401 gaacggcagg ttgtaagggt agtcactccc ggtacatttc ttccaggaga gtcctctgcc
     5461 gagggtcgta tagcggctgt ggccccacag gggaacgata tttcacttgc ccttcttaac
     5521 tgcgcttcgg gagtaataag tgccggaacc ttgtcaaggg agaccgcgaa agcggaacta
     5581 gccggttttt gccccaaaga aataatcgcg cctcagggtt ttgatgagca agccaggggc
     5641 ctgatgcctt ttggccggga gtatgttttt accccgagaa ggaaagagga attcaacccg
     5701 gtcgatgctt ccaggatgat ctgcaggaga atgggcgttt ccacccttgc cggttttggc
     5761 attgaggatc tggatcaggc atgtggttgt gccggtgtcc tgtataccta catagaggag
     5821 acccagttcc gatcccctga gggagtgctg gctttttccc ctctgcaaga actgtcctcg
     5881 atgaggatag acccgtcctc tatagagaac cttgaaatag ttggggaaga aggggcatct
     5941 cttgtcggga cacttgacag gtgcaggacc cccggaggaa agagaaaact gcgtgaatgg
     6001 ttattgcacc cccttcttga tccagcggag atatcccgaa gacaggacag ggtatcagcc
     6061 cttgtggagg cctctggatc cagggcagcc ataacaagtt ccctttccgg ttgtggcgac
     6121 cttgataggg cgatctcccg tttcgccttc gggagcggag ggccaaggga tgcgttgacg
     6181 atcagggaaa acctgaggat tcttccccgg ctcaaggcag ctctggaaac agccgggatc
     6241 caggacctgc tcgaacttga cgaaggtgtt ggatcgctct tcgatcttct ggacaagtcc
     6301 ctgaaggatg agctcccccg catcgcatcg ggaggggaac tcatcaggga gggttttgac
     6361 gaggatatag attctctccg aaagatgaaa aaaggtgaga gccaatggct tgtgaatttt
     6421 gtggaggaac taaaaaaaga aacgggtatt aaaaacctca agacgggtta taacaaggtt
     6481 tttggctact acatcgaggt tcctcgatcc tctgcttcga tggttccgga ttcttttgta
     6541 aggaaacaga cgcttgtatc gggtgagcgg tacgtgacgg aagaactgaa aagcttcgag
     6601 gagaggatga tctcggcttc ggatgaactt gtaaggcgcg aagaaaaggt ctttcgggat
     6661 gtttgccgtt ttattgtgga aatgtcctcc aggctaaggt gcgtggcttc cgccatggcg
     6721 gccatcgatg ttttcgctgc ttttgcccag atatctttga tgaatagata ctgcaggccc
     6781 tttgtcgatc atggagattc gatattcatt gagggagcca gacaccctgt tgttgaaagc
     6841 cgaatggccg gggatgtttt tactccgaac gacatttccc ttgactccgg ggaaagccgc
     6901 attgccatag tcaccgggcc aaatatggcg ggaaaatcaa cgtatctgag aacagcagca
     6961 cttctggtga taatggccca ggccggttgc tttattcccg cgagaagcgc gaggctgggg
     7021 gtagttgaaa aagtattctc ccgtataggc gcaagagatg agatagcaag gggaagaagc
     7081 accttcatgg tcgaaatgat agagaccgcc aatatcctga acaacgttac cgccaaaagc
     7141 cttgtcgttc ttgacgaggt tgggagagga acatccacct atgacggtat gagcatagcc
     7201 tgggccgttc tggagtatct tcaacagcat gcgggaaaaa ggccaaaggt tctttttgcg
     7261 actcactttc atgaactgac cgcactttcg gagacacttc caggagttac gaacctgagt
     7321 atggccgttg aggaaaggga caacggggtc attttcctgt acaaggttgt cccggttcct
     7381 gcggacaggt cctacggaat agaggttgcc cgtctggcgg gtatcccgga agtggttatt
     7441 gaaagatcca gaaagatcct ggaaggattt gaaaaaagaa aggcagcatc aatggaaaac
     7501 ggtgcggaag aaggggcaga agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnngaagaa ggggcagaag aaacagggca actctccctt ttcgggggtg cggcggagga
     7681 gataattgat gaacttgcag aaatggatac cgatagcatg acgcccttcg cggcactgga
     7741 aagactatat ttactaaggg aaaaggcaaa gggggttcgt gggaagacat gaggccaatc
     7801 aggcacctgc cggcagatgt ttccagtcgt atcgctgccg gagaggtaat agagaaccca
     7861 gcttcggtcg tcaaggagat gctggagaat tccatagatg ctgaagcctc gaggatttcc
     7921 gttattcttt cgaacggagg gaaaagcctg atctcggtca tggatgatgg gaccggtatc
     7981 cccggtgagg agatgcccct tgcggttgaa cgattcgcga cgagcaagat ctcgactctt
     8041 gaagaccttt cgggaattct gtcattcggg tttcggggcg aagccctggc aagtgtctgt
     8101 gccgtctcca ggatggagat gcacagttgc cggaagggat taaaaaaagg cgcgatgatt
     8161 cgttgcgagg gaggggaaat aaacctccat gttcaggtgg acgcaatgga aggaaccagg
     8221 atactggtaa gggatctttt tttcaatctt cccgcaagaa gaaagtttct caagggatcg
     8281 gcggcggaac tcaggaaagt tgcttctgtt gtgagggatt attcatctgc ttttccggag
     8341 ataacctttg tgctcgagtc ggaaggacga aagatatggt caaccgaggg agatggggaa
     8401 agggtccgtg ttctgggcag aatgtggggt tccgaggatg tcagaagcgg atctgaaact
     8461 gcaggcgccc tggagataga ggcctactgg tcccctgttc ccggctcaag acgaaaggat
     8521 gtcatcattt tcgtaaacgg aaggcgcgtt cgtgaaccgg cgatccttgc ggccctgtca
     8581 tcctgcggag agggggttgc cgggaactgg ttcttttcaa taaaggcccc tggagaaatg
     8641 gttgatgtta atgtccatcc cggtaaggcg gaggtaagga tccatccatc gataccgctc
     8701 tttgaaacaa ccagggctgt cgtcggaagc cttttgggtg gcttttcggt gggaaataaa
     8761 agcattgaga cttcatccgg gactgaaacg aaaactgcat ttcgccaggg agaatggaat
     8821 gatctccggt cactggatca tgacggatcc tttttttcca gggtagctga accaccggca
     8881 tatgccttcg gtgaaacaag cgtcgcagca ggaagaaaag gttattcttt tattgggcag
     8941 atatcctccg gttatctgct ttttgaaagt gagggagacc tggttcttgt agatccccat
     9001 gccgctcacg aaaggatcaa ttatgaaaaa ttcttaaagg caaggtcgga ggctgtcccc
     9061 cagagcattc cctttcccga acaggtgcct ccgtcacttt cgctgcgtgc agccgaaata
     9121 agggattcgt tggaaagtgc gggtttcagg tttgaagaaa gggatggaac acttttcatc
     9181 aaggcgctcc cggaaggatg tggctcagtt tcctcgaagg atcccgtcga ggctcttcgt
     9241 gtgtgggccg ggttaatcga gaaacccggc tgttttcccg atggaaaggt cccgatggca
     9301 tccgaggcct gtatggcatc ggtgaaactg ggtgagagga tcgcgatcga agaggcgctt
     9361 aaactgatct cggatcttct ggaatgtcag gaaccctttg cctgtcccca tggtcggccc
     9421 gtaatggcaa gacttggatc gtcggatctt gccaggctct tcgggaggga aagcaagtga
     9481 agataccgga gaattctgaa ctgctatgtg ttatcggtcc gacagcctct ggtaaaacag
     9541 ccctttccct tgaactggcg agatctcttg acggagagat catttccgta gattcccgcc
     9601 aggtctttcg tttcatggat gtagggacgg acaaggtttc gatgaatacc agagcagaga
     9661 tacctcatca catgatagat gttgtggatc ctgatgggta tttttctgcg gccgattttg
     9721 ccgaaggagc aaaaagaata gtggttgata tcgtctcaag aggtaaaacc ccgatccttg
     9781 tgggggggac acccttttat taccgagcgc ttttttcggg gattctctcc gccgaactga
     9841 agagctggcc ggaagtgagg aaagaatacg aagcccgatg gcgcgatgga gaagcaaagt
     9901 tcctttacca gcgacttgaa agagaggacc ccgactacgc ctcccatgtt caccccaatg
     9961 atgccttcag ggtgatccgg gcacttgcga taatagacaa aacaggaaaa aaaccgacct
    10021 actggcgcaa aagggctctg aaaaggaaaa ccggtctttc gcccttttat ataggcctgt
    10081 acccgggaag agaggccctt tattcaaaca tcgagaaaag ggtaagggaa cagttcctga
    10141 aggggtatcc cgaagaagtc gaaaacctcc ttgcccgagg ttacggagaa gaccttcctt
    10201 ccatgagagg ttttggctac agggaacttg cggctttcca cggtggccga atgtctttga
    10261 aggaagctct cgaaggggat attcgttcca caaaggcttt cgcgaggaga cagatgacat
    10321 ggtttcgccg gttttccccc agcctgtggt attatgtccc aggttcagga attacagaac
    10381 aagtgaggaa agtgataggt ctttggagaa caagacattc gtaattcccc gaaatacgga
    10441 actggtgttt gccgagccca gaggtttctg ttttggcgta agaagagcca tatccaccct
    10501 tgaggaagcg ctggccaagt tcggcagggt ttatgctctg ggcagtccca tacataactc
    10561 ccgcgaaatt gagagactga ggtcaatggg cctcgagata gtggaggatc catccgaaat
    10621 cccccagggc ggggtggtct ttattcgtgc acacggagtt tccgacaggg ttgtggaaca
    10681 tcttacccaa aaggaagcta acatcataga cgggacctgt ccctttgtca gaaaggccca
    10741 ggaaagggca acttttcttg ccgaagaggg ttaccatgtg ataatactcg gagacaagga
    10801 acatccggaa gtcagagcca tcagggagtt tgcccgaggg cccgttacgg tggtggatcc
    10861 ttttgacata ttaccggcgg gcaataccgg ggcgtcgagg ataggcatag tttcgcagac
    10921 gacgcagaga agggagtctc tctcttctct tgtttctttt gtctgtgcaa ggacccctga
    10981 ggtgagggta ttcaatacca tctgtggtgc cacagcctca agacaggatg cggtcgcgac
    11041 cctggcggac aaggttgatg gtattatcgt aacaggtggc aaaaacagtg caaatacggc
    11101 taaacttgtc gagatatgtg aaaacgccgg aacccctgtt ctctggatag aagatgaggg
    11161 ggagatggaa agcgggtggt tcagggataa aagaacgata gggatcgccg ccggggcaag
    11221 cactcccgac tggctgatcc acagactcga aacgaaacta gcgtctgcaa agagcgccag
    11281 ggaggatgaa tgggaagatg actgaggaga aaagaaacca gctggaagaa gaacatgttt
    11341 cttccggaac tattgaacca acagaaataa cagaatcaac agagtcaaat gaggttaatg
    11401 atccggctga aacaatggaa agctttctgg agcaatacga ggtgggtcag atccatcgcg
    11461 gcaagaccgt tgaaggtacg gtagttgagg cagccgaaga aggctggctt attgacgtgg
    11521 gcttcaaatg cgaaggattt ctgccaaagc atgaatggtc tcatcgggtc cttgtcgaat
    11581 caaaagagac tccgtctgtt ggagatactg taagggttca ggtcgtctcc atcaagcagg
    11641 gcgaagaatc ccagttgctg gtcagcaggt ggagaaccga attcgacgaa cggtgggaag
    11701 cgatggaaga aagtctctca aaggacgaat tcatcgaagt ccggggcctg agaaaggtca
    11761 agggagggct gatcgttgac tgctgcggca tagagggatt tattccggta tcgcatcttg
    11821 ccgaagaggg gcggggagtc aacccttccc gtcttgttga ggattccttt tctgccaaag
    11881 taatcgaaag ggacagaaag aagagaagag ttgtcctttc acgaagggcc atcctcgaga
    11941 gcgaaatgaa cgaacagagg gaatctttct acaagcaggc cgaaataggg caaatagtag
    12001 aagggacggt ctcttcgata acctcctttg gggcatttgt taacatagga cctgtggatg
    12061 gactggtaca caacacagaa ctttcatggg gaaggaacag caaacccaga gaaatagtaa
    12121 acaagggcga ccgggtaaat gtaaagatca ttggaataga accggagaac aaccggatct
    12181 ccctgagcat aaagcagacc caacccgatc cctgggaaga ggcatcttcg aggttcaaag
    12241 agggtgacag ggtaaccgga agggttacta acctcacgga atttggcgct tttgtcgaga
    12301 tcgagccggg tatagagggc cttgtacata tcggcgatat cagttggcag aggatccgcc
    12361 atccaaagga agccctccgc aggaaccagc agattgaggt ttgcattctt ggcatagatg
    12421 aggccggaaa aagaataagc cttggtttca aacagctgaa cgatccctgg agagatatag
    12481 atgaaaaatt ccaggtaaac ggtgattaca ccgtcaaggt cgtgaggctt actgattttg
    12541 gagcctttgt cgagcttgag gagggcatcg agggccttat ccatgtatcc cagcttagtc
    12601 gcaagagggt cgagaaaccg ggggacattc tcggagaagg gcaggaagtg ttggcccgca
    12661 tcatcgagat caaacccgat gagcgaagga tcaggctcag catgagcgct ctggaagaac
    12721 cggagccaaa acgggagcgg gaagttcgtg aacccgacaa acgcagaaaa aagagtaacg
    12781 ttaaaagcga cagcagccag ttcatggatg gagagaccaa cgttacaatt ggtgatctct
    12841 ggcaagaggc gctgaaggac taaagatcag gttggggcgc gtgatgcgcc ccaaccgcag
    12901 cattttactg gttgaaagga agcgtttcca gatgtctgtc caccgaattc gggaatatcc
    12961 cgacccggtt ttgatgagca ccgccgaaag ggttggagaa ttcaacgagg aactgctgtc
    13021 tcttgttgat gatatgtggg agaccatggc tgaatatgac ggagtaggcc tggcagctcc
    13081 gcagataggt tttggcatcc aggtcgcggt ggttggctgg aaagacagaa agataattct
    13141 cgtcaaccct gaggttctgg aagtggaagg ggagaccgtg caggaagagg gttgtctcag
    13201 cgctcccgga gtatttgaaa aagtagtccg tccggatcgt atagttgttc aggcacagga
    13261 cgagaaagga aagcctcaaa ggatagagga gaccggcttt cttgcaagag ccataatgca
    13321 tgagatagac catcttcatg ggcaactgtt ttttgacaga ctgtcaccac ttaaaagaaa
    13381 atttcttaaa aggaaacttc agaaaagggg ataaggatgg atctcgggta tcctgtatgg
    13441 tttgccggat ctggttcctt tggagcgaac tgcctggcta atgtttctga agaagtatgc
    13501 atttcaagag ttattaccgc acccccaaaa ccggcaggga ggaaactgga aaaaaggata
    13561 acacctgtcg aatcccgggc ttgcaccctt ggattaccaa ttacccggac ggaaaacatc
    13621 aatactgatg taataatcag ggaccttttt gaaaaggaac ggccatgctg cgttgtggtt
    13681 gttgattttg tacagaaaat cggggaacca tttctttcca ctgggcctcc ggggtgtttg
    13741 aatattcatc cttcactcct ccccgcctac aggggctcgg ctccggtaca gagggccctg
    13801 atgaatgggg aagtgattac cggtgtcacg gtgttcaggc tcgtagagga aatggatgcg
    13861 ggacctgtaa tggggtcggt agaagtggaa atcgggcccg atgatacttc gggggatctt
    13921 tttgaggttc tttcgttgaa gggtagccga ttgcttgtag atggcctaaa attgtacagc
    13981 aagggaagga ttccctttaa agagcaaagg catgagaaag cgacttttgc ccccaggata
    14041 caaaagagcg aaaccaagct gagttgggaa aaaccttcca tctaccttca taacctggta
    14101 agggctatga atccctcgcc cggtacctat gttttaatca acgggaaaag actcaagatc
    14161 tggaagacac gtccccacca ggagtcggga gaaccgggtg agatacttga ttctgttccc
    14221 gggaacccgg ttgtagcgtg tcgtgaggga tcaattgagc tgatagaggt tcagcaagaa
    14281 ggcaagaaca gggcagatgg agcgtcctgg ataaggggta cttcattcag aaagggtgat
    14341 atcctttgat tcccggaaca aggaaaccca tgcctgcagg gccgatctgc gggcgacaat
    14401 ggcccagagc catagcggtt acaggggcgc tcggttccgg caagacagaa tgcgttctca
    14461 atatggccct ctcttttcat gaatgcggtg aaaaaataac cattgccgat gttgatatta
    14521 tcaacccgta tttttgtgtt agacagatcg cagaaaacct gagaaggatg ggttttacta
    14581 ttctcactcc caaagaaagc gcgagatggt ctgacatgcc cgttatctca cctgaagtgg
    14641 gttgggctct caagaccgag ggaaagagac tgatcctcga tgtcgggggt gatgctgtcg
    14701 gggtcagggc cctgaagcag tacagcgaac ctctggagga ggcggggtat cttctgatcc
    14761 tggttgttaa tcctttcaga ccacagacgg gggatgtgtc cggcataagg aatatgatcc
    14821 ttaacatgga aaagctgtcg ggtctgaaaa taggtgccct tgcggccaac cctcacctta
    14881 tggatcagac cacatcagcc gatctgctct ggggttaccg gcaggtatgt gacgcaggca
    14941 aagatattgg acttgaagtc cttttcggaa cagttccgcc tcatctggaa agcgaatccg
    15001 attatattgg gaaagagtct ccggttgatc ttcttttcat gacaaggcac atgatgcttc
    15061 cgtgggaaga gggatacatg tggacccagg ccggggacag gtcatagaag gttgtctctc
    15121 cggtgttgcc ggaaggtggt aaccattttc aaaacgaaag aaggtgtgcg gttaaatggc
    15181 aaagggacgc atcaacgttc ttgctgaatt ctgcaaaagc tgcggcctct gtgtacaggc
    15241 ttgtcctgtc aaggttttga ggatatccga gaccatcaac agcaagggat accggcctgc
    15301 ggaacagttc aaggaagggt gcatagcctg cgggatgtgc gctaccacat gtccggacgc
    15361 agccatcgaa gtattccgga tcgaagaata gaaaaaggct gggaggtgtt atctatggca
    15421 agggttctta tgaaaggcac cgaggcaatt gctgaggcgg cgatccaggc cggatgtaaa
    15481 tattttttcg gctatccgat cacgccccag aacgaaatac cagaatacat gtcggggaaa
    15541 cttcccaaga taggcggagc ctatgtgcag gccgaaagtg aagtagccgc gatcaatatg
    15601 gtcatgggtg gcggctcgac gggtaccagg ataatgacaa cgtcgtcaag ccctggcgta
    15661 tctcttatgt ccgagggtat cagttacata gcagggtgcg aaatacctgc tgtcatcgtc
    15721 aacgtaatgc gcggcggacc tgggctgggt ggaatacttc catcccaggc agattatttc
    15781 cagtccacca agggcggtgg aaacggagac tacaggctcc ttgtaatggc tcccagcaca
    15841 ctccaggaag ccgttgaggt gacccaggaa gcttttgacc tggcccagaa atacagaaac
    15901 cccgtaatga tacttgccga cgggttcatg ggtcagatga tggaagcggt ggagatcaag
    15961 gaacatgtct cgaaggatct tcctcccagg gaaccatggg ctcttgggtg catgggtgag
    16021 aggggaagca aaaggaatat cctcaaaagc cttttcctca acccggaaca cctcgaggag
    16081 cataatataa aactgcagga aaaatacaaa ctgatggaaa aagaagtcag gtttgagcag
    16141 tatcagtgcg aagacgccga tatcattcta gctgctttcg gcacaacggc gcgaattgcc
    16201 cggtccgcca tcaacaacct gcggaaagaa ggagtcaagg tcggcatgct tcgaccgata
    16261 acacttttcc cgttcccgaa ggacataatg gcaaaactcg ccgaaaaggc gaagcatgtc
    16321 cttgttgtgg agatgaatat ggggcagatg gtcgaggatg ttcaggcagc gatttgcgga
    16381 aaggcccctg tcgccttttt cgggcacgcc ggcggtatcg ctccttccgt cagggagatc
    16441 gaagaccaga taagggccat aatctaggcc ggggaggtgt cgaacagatg aaggaaacca
    16501 agatctatga aaggccaaaa tcctgggtaa aggaagtgca cacccattac tgcccgggat
    16561 gcggccatgg tattgcccac aggctgctat gcgaggttat cgacgaactg gggatccaaa
    16621 atgacacgat agggacggct cctgtaggtt gtgcggccct catgtacgac tatatagatg
    16681 tcgatttctg tgaggccgcg cacggcaggg cgcctgcact ggccaccggg atcaagagaa
    16741 caaggccaga caagatcgta tttacctatc agggcgacgg agaccttgct tccattggca
    16801 cagccgagat catccatgtc gcaaaccggg gtgagaaact gaccacgatc ttcatcaata
    16861 acgcgatcta tggcatgact ggcggacaga tggctccgac cacccttctt ggccagaagg
    16921 ctacaacatg tcccttcgga agggaccctc tgcttgccgg gtatcccatg aggatgtgcg
    16981 aaatgctggc cactcttgaa acacctggct atataaccag ggtttcggtg gcaaaaccca
    17041 aatatattat cgaggcgaag aaggccatca aaaaggcttt ccagaaccag atcgacggaa
    17101 aaggatactc ctttgtcgag atcctttcca actgtccgac caattggggt atggcaccgg
    17161 tgccggcttt cgagtggacc gaaagcacta tgatccccca ttaccccctt ggcgtgttca
    17221 aggaattcaa ttaaggaggc ctgcagatta tgtccggttt cttcagagga atgatagcgg
    17281 caggtttcgg aggccagggg atcatggtac tcggtcagct tgtggcctat accgcaataa
    17341 acgaaggcag atatgttacc tggatacctt cctatggccc ggaaatgcgc ggcggaacgg
    17401 caaactgcgg agttgccatc agcgacgagg agattgcgtc tcctgtggtt gcagagtcgg
    17461 atgttaccgt aataatgaac aacccctctc tcgaaaaatt cgaaaaaacc gtcaggccgg
    17521 gaggcgtcct tgcctataat tctgatcttg tcaaatacga cagtcccagg aaagatgtaa
    17581 tattgcttcc tatccctgcg cacagtgtcg cttcggctct tggaagcgac aggatatcca
    17641 atattgtggt ccttggtgct atcgtcaggg cgacggacct gatctcccgt gaagtttgta
    17701 tcgagaccat caaggataaa ctcggctccc ggaaaccgca gttccttccc atgaacctcg
    17761 aggcctttga aaagggtcag gagatagccg aaaaactcct gaagaagtag gcctgtaatg
    17821 ggctccgggg aggaaaagat ctcgggtacc ctggtttatc gggggagaat aatcaacctt
    17881 cgggtagaca acgtcaggtt acaggatggc aacaaggcct cccgtgaggt tgttgaacat
    17941 gctccggcag tagggatcat tgccgaggct gatgatggcg acctttttct ggtgagacag
    18001 ttccggtatc ccgcggggga gtttttgcta gaaattccgg ccggcatcgt cgagcctgga
    18061 gaaaagcctg cggatacggc gagaagggaa cttcaggagg agaccggttt cgacgcaagg
    18121 gatattgtcg agatctcccg gttctacaca tctccagggt tcagtaatga gatgataata
    18181 ctttatcacg cctcggggct tttgccgtct aaaatggaat gcgatccaga tgagtttctt
    18241 gatcttgtga ggatgtcaga gacggaactt ctcgaggcgg ccgatacagg aaagatcagg
    18301 gattcaaaga cactttcggc cgtctactgg tacctcgcga aaagatcggc catctctcgg
    18361 aaaagggacg ggtaaaactc gccccttttt cacgggagcg gaatatatgg aaaaaaacga
    18421 taaggatctg gctctttttt tcagctatct gcgatacgag agaggctgtt ccgagaacac
    18481 catccaggcc tacggctctg accttagggc atggaggaaa ttctgtggtg agaccgggac
    18541 agccttttgt ccaccagagc gggatggtct ttcctctttc ttttctacaa tgacgttgtc
    18601 cggaaaaagc aagtcttcga tccagagggc tgctgcaact ctgaggacct ggctgaggtt
    18661 tcttgagacc gaaggggaaa cagaaagtga cggctctctt ccccggttgc ccacccgcga
    18721 aaaaaaactg ccccgtatcc ttacggaagg ggaggttaac aggctgatga cagcctgcga
    18781 aggtgatgat cctctctcac tcagggacag ggccattgtg gagctaggtt acggatgcgg
    18841 attgagggct ggcgagatat ccgggctggt tcttcgtaat gtggggtttg attcaggttt
    18901 tctcaagccc ggcggaaaag gtaataagga gaggatcgtt cctcttgtgg gcaaagtaag
    18961 agcaagtctg gaaatgtata tatcccgtag cagggaaacc ctgggggcac ccgccaccga
    19021 cagggtcttt gtttcggtca acggtaaccc cctgaggcga gaggatgtct ggaggatcat
    19081 aaggaaaagg ggcaaagagg ccggaattgc cgaatcccgg ctgtatcccc atatattgag
    19141 acattccttt gcgacccatc ttctcaggag ggggatggat cttcgtacct tgcaggaact
    19201 tcttggacat tcctccataa tgaccacgga aaaatacact catttcgata ctgaactcag
    19261 ggacgtttat gataaaagcc accccagggc atgatgagcc tgtacctggc gtggatgaca
    19321 ggggagggtt tcaggatgac cgattacagg gaaaaggttc gggaagccag ggaatatgtc
    19381 gagaaaaggt ttaaacacag tcctttggcc gtttttgttc tggggtctgg ccttggggcc
    19441 ttcgcggatt cgatacagga aaagatcgag attccctacg aggatattcc tggatggccg
    19501 gaatcaacgg ctccaggaca ttccgggaaa cttgtatgtg gatttatcgg tcaaaccccg
    19561 atagtggcca tgcagggaag acaccacttc tacgagggat attcaatgtc ggaggtcgtg
    19621 tttcccgtga gggtcttcgg agagatgaaa gtacccaact actttggcac caatgctacg
    19681 gggggaataa gtcattctct ttcccctgga gaccttgttc ttgtttacga tcatatcaat
    19741 tttcagggac acaaccccct tcggggaccc aacgaggatt tatggggacc gaggtttcct
    19801 gacatgacct atgcatatga caggggactt ctggaactgg tagaggctgc tgcctcctct
    19861 ctgaacctgc agatcaggag agggatatac gctgccttcc cgggcccatc ctttgagact
    19921 ccggctgaga taagaatgat gaggatactt ggggcagacg tcgtagggat gtcaactgtg
    19981 ccggaagtaa ttaccgcgag gcatatgggt atgagatcct gcgtgatctc ctgcgttgcg
    20041 aattatgccg ctggaatgtc tgaaaaggta cttacacacg aagaagtcct ggaagagatg
    20101 gacaaggcat cgggaaggct gacaagcctc cttcaggccg ttgttctgaa acttggaggt
    20161 gagatcaggt gaatcctgtc tccttcattg aaaccaaaag ggacggtctc gaaaactccc
    20221 ctggtgatat cagggaattc gtccggttgg ccttaagtgg aggaatgaaa gattaccaga
    20281 tctcttcctg gctgatggct gtatttttca ggggtatgac tccttccgag aacaggacct
    20341 tcacagaggc cctggctggg tcggggaaaa cagtccggtt ccccgatggt ctttttgctg
    20401 ttgacaagca cagtacaggg ggggtaggtg acaaaacaac cctcgtggta gtgccccttg
    20461 tggctgcctg tggtgttccc gttgccaagc tcagcggcag gggtcttggt tttacaggag
    20521 ggaccgttga caagcttgat tcgatccccg gtttccgttc gagccttgac atgaagaatt
    20581 tcattgatca ggttgaaaag accggatgtg ccgtcagcgg ccactcaaaa gacctggcac
    20641 cggcagaggg agtcttttac aagctgagag atgtgaccgg gacggtgcca tgtatccctt
    20701 tgatcacgag cagcatagta agcaaaaaag tggccggggg atcaagtgct ttcgttttcg
    20761 atgtcaagtg cggaaatgga gcgttcatga aggaccttga ttccgcgaaa caactggcaa
    20821 gacagttggt ggatctttcg atggaccttg aaagaccggc catcgcaatg ataaccgaca
    20881 tgtcgagacc tttgggaaga tggacgggaa acgccgccga agtgatggag gcgatagatg
    20941 ttctgcgagg cgaaggccct gaagatacgc tggagctttc tcttcggctg gcgggggcca
    21001 tgatccacct ggggggggag gtttcatcca ttgaggaggg tgtcaaaatc gcggaaaaat
    21061 cgattttctc aggcaagggt cttgaaaaat tccttgaaat ggtaaaagtc cagggtgggg
    21121 atcccgggga ttttctcgat gctggaaaac gcagaaacca ccttgctcct tctgttttcg
    21181 agatcgaagc ccttgaagcg ggctgggtaa cggagtgcga cgcgctgttg attggagagg
    21241 cggttcgctc tattggggga gggcgtctga ccagggagca ggaaattgat ccctctgtaa
    21301 gctgcgagat agtgacagtc aggggtgaaa aagtaagcaa aggtcaaccc ctggcgagac
    21361 tctattaccg aaatgacggc ccggagattg agaacgctgt cgaaatggtg cgccgtgcct
    21421 tccggacagg agagagaccc ggaacggaaa aactgtttct aggcttcgaa ggacctgcaa
    21481 cgtaataggt aggtcgtaat gtctggtgta aggaacctgt gggaagaacc ttcggggggt
    21541 cttgtcgaga ggtccggcaa gggggacgaa gaggcccttt cggagatgat gcttcgcttt
    21601 accccgctga tccggtatat tgcccgggaa ttcaagggaa gttttcttgc agaggaagat
    21661 gatctccttc aggaaggtta catctcactt atcagggccg ttcggggtta tgaggcaaac
    21721 cggggcaaat tttcatccta cctcttttca tgcgtaagga acgggatgat ctcatacctc
    21781 aggaagaaca agaacaaaaa cagggaatct ccacttgacc cggagacgga agcgagcgga
    21841 aagggcagtc tctgttggcc ggtagaagaa ctgcttccgc cggattttac ctcccttctg
    21901 acaattaccg aaatgaccgt tctcgatgcc tttcttgaaa ccgggtcagt atcttcttcg
    21961 gcggtggtcc ttggttggca aaggaaaaaa gtcgataatg ccatgcagag gatcaggaag
    22021 aaactgaggg gatggagaga tgcaagggct gaggcgtgag acgtgaaacg aaaaagcaaa
    22081 agaaaagggc gtgaacggta aacggtaaac ggtaaa
//
LOCUS       DAOTIQ010000029        22082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_2801_length_22082_cov_7.219503, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000029 DAOTIQ010000000
VERSION     DAOTIQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2801_length_22082_cov_7.219503"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="P5541_05220"
     CDS             <1..1367
                     /locus_tag="P5541_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRV71415.1"
                     /translation="YVLIFCIILIAAVATYIIPAGVYDRVTDPNTGRTVVDAASFKNV
                     PQNPIKPFAVMQAIPKGMNEAGWIIFLIFIIGGSFGMINGTGAIETGIAAMVRKLSGK
                     EKILIPVTMLIFSLGGATFGMAESTLIFIPMGVVLARGLGFDALVGMSMVTIGALAGF
                     AGGALNPFTVGVAQGIAGLPLFSGMGLRWITQLLFWAVGSFYILRYAMKIKSDPAQSI
                     VYELEQTVKEEESEQQHFTELTTRQKLVLLIVVVGFGFIIYGVTHGWSTSSDLTATFL
                     TMGILSGLVGGNSPGRVATDFIEGARSLTFGALVVGLTRGIVVVMESGQIIDTIIHAG
                     AQIVTFLPRSIAAVAMLAVQSCINFFINSGSGQAAATMPIMAPLADVVGLTRQTAVLA
                     FHMGDGLSNSLFPTSGVLMAGLSIAKIPYEKWVKYILPLMVALHLVAAAMMVVAAVTH
                     YGPF"
     gene            1530..2621
                     /locus_tag="P5541_05225"
     CDS             1530..2621
                     /locus_tag="P5541_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRV71416.1"
                     /translation="MKIAVLGSGNGGTAVAFDFASNGHDVYLYDLPQFNENISAIREA
                     GGILSEGRMQGFAKVHYSGHDLEKVLTGADITFVVGPSYAIAPFARACAPFLKEGQSV
                     VICPGSCGGSIVFLRNLGEDLSSEKFRLAETHTLPYAVRIVGPARIQVYNKLRGGLFL
                     GGLPRSVGSEILEKINPVYPGMTLASNIFQTFLQNSNPILHPPVVLMNAALIERTGGN
                     FMFYEEGVTPAVGKIIEALDQERISIGKALGVDIIPNTRIGFLQGYQEEPDTDYFTLY
                     SKGSGFRGIGAPSSLDHRYLTEDVARGLVLFHGVARKAGVGTPVMDSVITLVSRILQE
                     NLFDHVDRRLEEIGLSERSIEEFLKLINR"
     gene            2663..3847
                     /locus_tag="P5541_05230"
     CDS             2663..3847
                     /locus_tag="P5541_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV71417.1"
                     /translation="MDAIGSAKAIHKKVVEWRRELHRIPETDFDLVKTSQFVKDRLDE
                     FGVEHRVMGRTGVVGLVRGIKDSPTIALRADMDALPINEETGLPFASKNGKMHACGHD
                     AHTAMLLGVARILSENRESLKGNVKLVFQPAEETTGGAKPMIEEGCLENPKVDAIFGL
                     HIGHLFREVGNGQIGVRFGEMMAAVDSFIVKVRGRGGHGARPHQCVDPVTTAAEMIMG
                     LQKIVSREIDPTHGAVVTVGMIKGGTTTNVIPEEVEFRGTIRTLNPDDRQFIEKRFAE
                     MCSSIAEANRAKAEINYANYYPAVINDEEMTRVLAGAAAKIVGEENVVEIRSPSMGAE
                     DMAYYLQRTRGSFCSLGSGKKDGNNEYPHHHPKFDIDEDVLWIGPALFVQTVLDLSDG
                     MI"
     gene            4069..5403
                     /locus_tag="P5541_05235"
     CDS             4069..5403
                     /locus_tag="P5541_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRV71418.1"
                     /translation="MAITKEDLIKKARKPAEDAMKLHPFYRGKMQTAPKCCIRDINDF
                     AIWYTPGVAAPCKAIKEDIDLSYEYTNRGNLVAVVSDGTRVLGLGDIGPEAGMPVMEG
                     KALLFKYLGGVDAVAICLDTKDPDKLIETVRLIAPSFGGINLEDIAQPKCFHVLDELR
                     KDCKIPVWHDDQQGTAAVNLAGLYSGLKITGKKIDEAKVRFFGAGAASIATARILISA
                     GVRPENVIMADSKGTLHKGRTELKDVPYDVKWHFAQTTNPEGIEGGPEVLFRDADVVY
                     CYTKPGPDVVKKEWVATMAKDAMLFACANPIPEIWPWDAKEAGARIVATGRSDFPNQI
                     NNSLGFPGIFRGTLDVQASTITDEMCIAAAKTLSKCAEKNGLSEDYLIPTMDQAWVYP
                     EVATAVGMKAIEQGIARKVLSGEELLKTATEKIQYAQKMTACLTEKKFIELP"
     gene            5705..6160
                     /locus_tag="P5541_05240"
     CDS             5705..6160
                     /locus_tag="P5541_05240"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HRV71419.1"
                     /translation="MRSIRRRSLVILAVLAALVLVFAERGFWGPSAIRVPEQVSAGAE
                     LLESLYRERKGGVQAEAGGIVTRLLPDDDRGSRHQRFVVELPTGQTLLIAHNIDLAPR
                     IDGLRTGDRIEFSGEYEWNPEGGVIHWTHRDPSGKHRPGWLVHEGQKYW"
     gene            complement(6167..7138)
                     /locus_tag="P5541_05245"
     CDS             complement(6167..7138)
                     /locus_tag="P5541_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV71420.1"
                     /translation="MKILDLKITPGVSVSLIDLPLDLDGYHDFFGIWLVRDEGRERNT
                     LVDVGPTSTASILIEELHTLDVKKLDYILLTHIHLDHSGGLAELLRAFPSARVAVHPK
                     GKRHLVNPERLWESSLQVIPEMALAYGEPSPVDESCFIPEEVPVPGITAVDTPGHAPH
                     HRSFFYWTPEGQVLFAGEAASTFTRLDHLVPGAGERRYMLRPASPPRFYIETALRSIE
                     ELKGIDASLLCYAHFGCTEQVREMLDHAGEQIRLWRKLFSEFLNMKGYKFENQVEMGE
                     LLGFVVERDPWLADFSLLPADVRSREMRFMLSSAAGFLGAVLEERKV"
     gene            7335..7967
                     /locus_tag="P5541_05250"
     CDS             7335..7967
                     /locus_tag="P5541_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71421.1"
                     /translation="MRERTKTDSVYEEIKKLILRGELSSDTPISENYLAQRFGISRTP
                     IREALKRLDMEGFVRIIPNQGVFVRDVSISEVKEIYDLRIALEEFVVKELTHTLSEDD
                     LFVLEGNLARQEEALRRHDAFAFHEEDRKFHDYFMKAYGNDMITDFITNLRDRIEGIN
                     VNMLKQPGNMELFWGEHRRILEALRRRDGEGATREMDEHLKGGKERLLRG"
     gene            8067..9125
                     /locus_tag="P5541_05255"
     CDS             8067..9125
                     /locus_tag="P5541_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV71422.1"
                     /translation="MREVNCPDFAGQDIKMRKLLTGILVLALLFGLASVDAASAALKG
                     EYKLTMNVTTDTAWGQGGVKFAELVKEYTGGKVNVKVYPSAQLVGGDQMRQAEMVSSG
                     AIDFMLNSTINIAPIIPECNAFSLPFMFPSYESVDAVTGSAAGQMVFDALEKHNMVGL
                     AWGENGFRELTNNVKPIAHPDDMKGLKIRVVTPIYIDIMRALGANAIAMNWGEVFTSL
                     QQGVIDGQENPIVGIIIPNKIYEVQKHLTNWHYSYDALILAVNKNVWESFDPDIKEQI
                     KKAAVDAMHWQVHDVSRVGLGDGIEFLKSQGMTVTDPTPEQLAAFREMTKAVYEKWSG
                     NVGADIVKAFEEAVSSFK"
     gene            9228..9731
                     /locus_tag="P5541_05260"
     CDS             9228..9731
                     /locus_tag="P5541_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV71423.1"
                     /translation="MFKLLDRIEEWTCVTMLFFMAMLAFVNVIVRYLTVFSFSFSEEL
                     LVNLFVWITMLGASIATRKGSLIGVSYLFDRMPPSLKKLSTAVFVLCGVTLFGLLTMH
                     GIDMVKSEFRSGMTTYSMALPMWIFGMAVPVCAFILILRTIQRGVHEWNDLSGPQTGS
                     CPEVDQR"
     gene            9728..11014
                     /locus_tag="P5541_05265"
     CDS             9728..11014
                     /locus_tag="P5541_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV71424.1"
                     /translation="MSIDPGVFLLGLFFIFLAFKVPIAVALGMAGMAVVYWYNLGAQM
                     FAPVFFANISKFSLLAIPFFILAGVIMGRAKISDKIIRLIMLMVGPVSGGMAIVTIIT
                     ALFWGAVSGSGPATVAALGTILIPGMVKAGYDRGFAAALTSSASGLAIVIPPSIGFIV
                     YGVITGASISELFLAGIIPGILVGLFLIVAAFIISVARGYKGDRFGTAKEVLTAFKDA
                     FWALLTPVIILGGIYGGIFTPTEAAAVGVAYGLFVGFVVYRTLTLKDLYELLVDACAS
                     SAVVMLVVAFAGIFGWAMMVLGVVERVSEGVIAMSSNPFVVIALINVILIISGMLLDA
                     ISIYYLLMPIFMPVIAHFGWDPVWFGVVMTINLAIGQVTPPVAVNLYVGCNVAGVSMD
                     EISRAVVPFLLASIAALAVIVYLPGITLWLPNLLMR"
     gene            11091..12089
                     /locus_tag="P5541_05270"
     CDS             11091..12089
                     /locus_tag="P5541_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRV71425.1"
                     /translation="MSAKKVKEKKGLNKYPRTLLEQFYRTMLTIRSFEKKVEEKFLAG
                     EIPGFVHLYIGEEAIAAGVISNLSREDYIQSTHRGHGHTIAKGADLKRMMAEIFGKKE
                     GYCKGKGGSMHIADLSIGMLGANGVVGGGFNIATGAALATKMQGKNNVSVVFFGDGAS
                     NRGTFHEAANMAAVWDLPVLFVCENNQYASTTPYMTTTSVENIADRAAGYGMPGVIVD
                     GNDIFAVYEESQKLIDRARRGEGPSLLECKTYRVKGHYVGDPERYRTKEEVNKVCESN
                     DPIDRFECTVLDGKLFSREELDAIKASAGKEVEKALEFARNAEEPEISELFAGVYA"
     gene            12111..13082
                     /locus_tag="P5541_05275"
     CDS             12111..13082
                     /locus_tag="P5541_05275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRV71426.1"
                     /translation="MREITFSQATLEAMDEEMTRDERVFVMGEDIARQGGIFGQFKGL
                     PDKFGKDRVKDTPISETAIVGAAIGAALTGMRPVADMHFADFMGVCMDEIFNQMAKIH
                     YMFGGQRSVPMVLRAPDGLINQAAAQHSQSIEAWFMHIPGLKVVIPSNPADAKGLLKS
                     AIRDDSPVIYFEHKALFNTRGAVPDGEVLSPIGKAKVAREGSDVTLVSYSHCMGLSLK
                     AADILAGEDVSVECIDLRTISPIDKETILASVAKTTRLVIAHEAVKQGGVGAEISAIV
                     AEEGIDFLDAPIVRVGAPFTPVPFARPLEQAYRVTPEKIAEAIRGLF"
     gene            13117..14619
                     /locus_tag="P5541_05280"
     CDS             13117..14619
                     /locus_tag="P5541_05280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRV71427.1"
                     /translation="MEQVTMPKLGLTMTEGTVSKWKVSPGDRIKKEEVLLVVATDKLT
                     YDVTSPIDGVVLEILVKEGESVPVGKVLAVLGEAGEKVSAADRQGERPEPGPEEAVER
                     PSTSIPLSRGVGTNEDVPGPESKGQTAAASPLARKWARSFGLDIGAIEGSGPGGRVVK
                     DDVMKASTRSRATPLARKIAREAGISVQEISGTGPMGRTTAGDVKAYLASPPKERIRV
                     SPVAARLAEDLEVPLGTIKVSGRIMKGDVLEAACAAKESKTVNRRVPLTPMRKVIAER
                     MTFSASTIPSVFFNIETDFTEFIGFRKALNEELEGSGVKVSFNDILMKICARALVEMP
                     MANASYDPGRNEYILHDDINIGIAVAVEGGLLVPNVKKVQEKPLPVIAMESLELVNKA
                     RKSRLLPEEMQGGTFTITNLGMFGIHDFIPIINPPEACILAVNVVTERPVCRDGQVVV
                     RPMSMIGLTADHRILDGADAARFLARVRELVEKPWMTLVQEWQTDKWSVN"
     gene            14604..16385
                     /gene="lpdA"
                     /locus_tag="P5541_05285"
     CDS             14604..16385
                     /gene="lpdA"
                     /locus_tag="P5541_05285"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRV71428.1"
                     /translation="MERELMATMITMPKLGLTMTEGSVLEWKKNEGDTLKKGEVVLVV
                     ATEKLTYEVEAPDDGLLLRIAVKAGETVPVGAILGAIGEKGEEPDLGVAVAEEVPTSK
                     DEAASEREQERPTGSLKGTEPAPAKGDTGKRVVVIGGGPGGYVAAIRAAQLGGNVTLV
                     EKERLGGTCVNVGCIPTKALLHSAEVNRTVEDAAEAGLKVGSLEVQWDVVQERKDQVV
                     KQLSQGIKALLSAHGVEIITGEASFMDDRNILVRSGEGDRKIESDAVIVATGSEASLP
                     PISGVELGGVVTSTGALSFDKIPSRMVIIGGGVIGVEFASIYADFGCSVTIIEMLPQI
                     LPGADGEAVSALRKVLEARGITIHTGAKVGSIEAAGSSLVISADLSGREEKIGADKVL
                     VAVGRKPFTEGLNLEAAGIRIERDRISVDKSMRTSVPHIFAVGDCCSPIMLAHVAMME
                     GEVAAGNIFGLDEKMDYSTTPGCVYTSPELAWAGLTEEEAKKRGHKVRKGVFPMIGNA
                     KSLISGETRGMVKIVADEKYGEILGVHIVGARATDLISEASLAIRLEATLDEVAETIH
                     GHPTFCEATREAVLAGLERAIHSIPRV"
     gene            16418..17410
                     /locus_tag="P5541_05290"
     CDS             16418..17410
                     /locus_tag="P5541_05290"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRV71429.1"
                     /translation="MKYIENRKTDPRFNLALEEVLFQSLDEKEEGFFLLWQNEPTVVV
                     GRFQNTLEEINVPVVREKGIHVVRRLSGGGAVYHDLGNLNFTFILRTENPQNLDFRKL
                     TEPVISALWSLGVDGGLVGRNDLVIQGKKFSGNAQYMREGRLLHHGTILYDSDMDVLE
                     EVLQADPDKFKSKGVKSARSRVTNIRSHLKGEPDIEVFRRALLFTVKERWPGLEISPP
                     DPSVQEKAAALAESKYGTWDWNYGASPEFDLRKERRFGFGKVDVRLNVRKGRITSCRI
                     FGDFFGNGDIAGLENGLAGTQFSEKGLSGMLKRVDVSHYISGMDSRSFMELVLE"
     gene            18044..18253
                     /locus_tag="P5541_05295"
     CDS             18044..18253
                     /locus_tag="P5541_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71430.1"
                     /translation="MKAKAEDAISAVRRSILDDRVPPLLEEKTVVPLFFGDARSCEGY
                     AVGHLDMGADPDQQQKVIPHCVGNG"
     gene            18475..19254
                     /locus_tag="P5541_05300"
     CDS             18475..19254
                     /locus_tag="P5541_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV71431.1"
                     /translation="MNRAGSFVVLNCALAILVALFSLSVIKGGAAEVLEGPLTISTSY
                     KSLLSTPDQKGMLDLLVKEAFSRIGLEAEIVFNPTGRSLSDVNEGLADGELNRIEGME
                     KNFPNLVRVPEPNMVMDFVAFSKKEYIINGWESISDLRIGIVKGWKILEENTAGFPDV
                     TLVPTETELFNMLDKDRIDVALYDRLTGYEQIHLRGFEGMFHLEPPLASREMYLYLNR
                     EHESLAGPLAQALRSMKEDGTWDRIVTETTAHLAFGGNVGD"
     gene            19277..21736
                     /locus_tag="P5541_05305"
     CDS             19277..21736
                     /locus_tag="P5541_05305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71432.1"
                     /translation="MNWRRSSTWRFFLYTLSGFVLVSIVVTGILGYYLFRHESGHLER
                     NVEQVEEIYLPFLIPALWVTDHESLQSQIEGIVKFSYIDRVEVRNDEGDVFAAGADPE
                     PSLEVISHDLVYSNRGQYRKIGSISLFVDRNELFSHIFEWMGSLFLLQLLLAVALSSV
                     MAFVYHMVIGRQLFRLAGFVKSADLTDPGKVFKLEHPSRQGDELQLLVDHINEMRGSV
                     RSHILEIQEWRDLLQYVIQHDPNAIMVLDRDMRFVFVSERLLKNRELERERVIGRTFM
                     EVLPELPEKWIAMFRRALEGVEQSSDLDYVARPDGSMDMISWWCRPWYEPDGSIGGTV
                     LYSEVITERKQMERSLFLEKELFRTTLLSVGDGIISTDDEGRILIMNRVAEELTGWDL
                     TEAREKDFKSVFHIIDEFSRERSPDPVRQVLDSGEPVEMEENCILVSRGGQETPVEDS
                     AAPIRDKEGETTGVVVVFRDCSEKRERQREIAYLSYHDQLTGLYNRRFFEEELSRLDV
                     PRNLPLSLVMIDVNGLKLFNDAFGHTAGDELLGKVAGVLREECRADDIVARIGGDEFM
                     ILLPLTDRDHLDPLMARISDAMSRERVKDLPISVSLGGATKTDVDESVEKVFDMAEDQ
                     MYRRKISEKSSYHHRSVNLIVEGLYSKSPEEKGHSENVSALSEAIGREMGLGQPDLDD
                     LKTAGVIHDIGKIGVSDRILEKSGPLDEDEWQEMKRHPEIGYSILSTVNEYSPFAEIV
                     LSHHERWDGTGYPKGLKRGEIPLFSRILAVAEAYDAMVSGRSYRKAMGREEAVAELRR
                     CAGTQFDPEVVDVFIEKVFGK"
BASE COUNT         5236 a         5563 c         6604 g         4679 t
ORIGIN      
        1 cttatgttct gatcttctgc atcatcctga tagcggctgt agctacctat atcattccag
       61 ccggagttta cgacagggtg acagatccga atacgggcag aacggtagtg gacgctgctt
      121 cgttcaagaa tgtgccgcag aaccccatca agccctttgc cgtcatgcag gcaataccta
      181 aggggatgaa cgaggcgggg tggatcatat tcctgatctt cattatcggt ggttccttcg
      241 gcatgatcaa cgggacggga gctatcgaga cagggatagc ggctatggtt aggaagctct
      301 cgggtaaaga gaagatactg ataccagtta cgatgctgat cttctccctt gggggggcaa
      361 ccttcgggat ggcggaatcg acgttgatat tcattcccat gggggtcgtt cttgcaaggg
      421 ggcttggttt cgatgcgctg gtaggtatgt caatggtaac gatcggtgcc ctggcaggat
      481 ttgcgggtgg cgccctcaac ccgttcactg tcggggttgc acaaggtatt gcgggtttgc
      541 cccttttctc cggaatggga ttaaggtgga tcacgcaact gttgttctgg gctgtaggat
      601 ctttttatat tttgcgttac gccatgaaga tcaagtccga ccctgcccag agcatagtct
      661 atgaactgga gcagaccgtt aaggaggagg agagcgagca gcagcatttc actgaattga
      721 ccaccaggca gaaactggtg ctgttgatag ttgtagtggg tttcgggttc ataatctacg
      781 gtgttaccca tggatggagc accagctctg atctgacggc cacattcctt acgatgggca
      841 ttctctccgg cctggtcggc ggaaattccc cggggagggt tgccaccgat tttatcgagg
      901 gtgccaggtc cctgactttc ggtgcccttg tcgttgggct tacgaggggt attgttgtgg
      961 taatggagag cggccagata atagacacca taatccatgc gggcgcacag atcgttacct
     1021 tcctgccccg gtccatagcg gccgtagcca tgcttgcagt tcagtcctgc atcaactttt
     1081 ttatcaactc cggaagcggt caggcggctg cgaccatgcc aataatggcc ccgcttgcag
     1141 atgtggttgg actgacaagg cagaccgccg tactggcttt ccacatgggt gacggtctct
     1201 ccaattctct tttcccgacc tcgggagtcc tgatggcggg acttagtatc gcgaagatac
     1261 cttacgagaa atgggtcaag tacatcctgc ccctgatggt ggcgcttcac ctggtggcag
     1321 cggccatgat ggtcgtggct gcagtgactc attacggacc attctagtca ttttcaaatt
     1381 atgctttgga caggtgatca cgaagagtaa cttcttcgtg gttacctgtc ccttttccca
     1441 gagggactgg tcaggaaact ttttcagttt ttcacctcaa gttttcacac caaattgctg
     1501 gtcccaatcg tggaaggagg accctcgaaa tgaaaattgc agtgttaggt tcaggcaacg
     1561 gcggaacagc ggtcgctttt gattttgcta gtaacggaca cgatgtttac ctttatgatc
     1621 tcccgcaatt caatgaaaat atctctgcga tcagagaggc cggcggaata ttatccgagg
     1681 gccgcatgca gggttttgca aaagtacatt attctggtca tgatcttgaa aaagttctca
     1741 caggtgccga tatcactttt gttgtagggc catcctacgc aatcgcaccc tttgcgaggg
     1801 cctgcgcacc ttttctgaaa gagggacagt cggtggtcat ctgcccgggg tcctgcggcg
     1861 gatctatagt attcctgagg aaccttggtg aggacctcag ctcagaaaaa ttcaggctgg
     1921 cagagaccca cacactgcct tatgctgtac ggatcgtagg gccggccagg atccaggtct
     1981 acaacaagct ccgtggaggt ctttttctgg ggggacttcc caggtcagtg gggagcgaga
     2041 tacttgaaaa gatcaatccc gtctacccgg gaatgaccct tgccagcaat atttttcaga
     2101 ccttcctcca gaacagcaac cccatcctcc acccaccggt agtgctgatg aacgcagccc
     2161 ttatcgaaag gaccgggggg aatttcatgt tctacgagga gggggtcact cctgccgttg
     2221 gaaagatcat tgaggctctg gaccaggagc gtatcagtat aggaaaggcc ctgggagtgg
     2281 acatcatccc caacaccagg ataggctttc tgcagggata ccaggaggaa ccggacaccg
     2341 attatttcac gctgtacagc aagggtagcg ggttcagggg tatcggagca ccgtccagcc
     2401 tggaccaccg gtatctgacg gaggatgtgg caaggggtct tgtgctgttt cacggtgttg
     2461 ccaggaaagc tggagtagga acgccggtta tggactctgt tatcaccctg gtctccagga
     2521 tcctgcagga aaatctgttc gatcatgttg acagaaggct tgaggagata ggcctctcgg
     2581 aacggagcat cgaagaattc ctgaaactga tcaaccggta aagagatact gggtcaatta
     2641 tgcatttaca ggaggcattg agatggatgc cataggttcc gccaaggcga tccacaagaa
     2701 agtcgtcgag tggagaaggg aactgcacag aataccagag acggacttcg acctggtaaa
     2761 gacctcacag ttcgtcaagg acaggctgga tgagttcggg gtcgaacaca gggttatggg
     2821 gaggacggga gtggtcggtc ttgtcagggg gatcaaggac agtcccacaa tagccctgag
     2881 agcagatatg gatgcgttgc ccataaatga agagacgggt cttcccttcg catccaagaa
     2941 cgggaagatg catgcctgcg gccatgatgc acatacagcc atgctcctag gggtagcaag
     3001 gatcctctca gagaacaggg agagcctgaa gggcaacgtc aagcttgtct ttcagcccgc
     3061 cgaagagacg acgggcgggg caaaacccat gatagaagag gggtgtcttg aaaacccgaa
     3121 agttgacgcg atcttcgggc tgcatatcgg tcatctcttc agggaggtcg gcaacggaca
     3181 gatcggcgtc cgtttcggtg agatgatggc tgccgtcgac tccttcatcg tgaaggtaag
     3241 gggccgcgga ggccatggag caagacccca ccagtgcgtc gaccctgtta cgaccgctgc
     3301 ggagatgata atgggtcttc agaagatagt cagcagggag atcgatccta cccacggcgc
     3361 ggtggtcacc gtagggatga tcaagggggg gacaacgacc aacgtgatcc ccgaagaggt
     3421 ggagttcagg ggaacaatac gtacccttaa ccccgatgac agacaattca tcgagaaaag
     3481 attcgccgag atgtgcagct ccattgcgga ggccaaccgg gcgaaggcag agataaacta
     3541 cgcgaattac tacccggcgg ttataaacga tgaagagatg acaagggtgc tggctggcgc
     3601 agcggccaag atagtaggag aagagaacgt ggtggagatc aggtcgccga gcatgggggc
     3661 ggaggacatg gcctattacc tgcaaagaac aagaggatcc ttctgcagcc tgggatcagg
     3721 gaagaaggac ggtaataacg agtatccaca tcaccacccc aagttcgata ttgatgagga
     3781 tgtcctttgg ataggccctg ccctgttcgt tcaaacggtc cttgatcttt ccgatggaat
     3841 gatataacaa aaatttgaat acttgtcaga ttttccgaga atattttcgg ttccagtttg
     3901 tatttatctg gatccttgaa attttttaga tccggggagg tactcgccgg gttgaacgca
     3961 gggggaggat atatcataat gccctaagta gaagcctgcc tgaagatctt tgatcttact
     4021 ggtgggacac ggtgtcccaa actgactcct tagggggaac tgaagataat ggcaataacg
     4081 aaagaagacc tgatcaagaa ggcccggaaa ccggcggagg acgccatgaa gttacacccc
     4141 ttctacaggg gaaagatgca gacggcgccg aagtgctgca taagggacat caatgatttt
     4201 gcgatatggt acacgcccgg tgtagcggca ccctgcaagg ccataaagga ggacatcgat
     4261 ctctcctatg agtacaccaa ccgcggcaac cttgtagccg tggtgagcga tggcaccagg
     4321 gtcctgggtc tgggtgatat aggtcccgag gcgggaatgc ctgtcatgga ggggaaggcc
     4381 ctgctgttca agtacctcgg cggcgttgac gccgtggcga tatgtctgga taccaaggac
     4441 cctgacaagc tcatcgagac ggtgaggctc atcgcgccat ctttcggcgg gatcaacctc
     4501 gaggacatcg cacagcctaa gtgcttccac gtacttgacg aactgaggaa ggactgcaag
     4561 atccccgtgt ggcacgacga ccagcaggga actgcggcag ttaacctggc aggactctac
     4621 agcggactca agatcacggg gaagaagata gacgaagcga aggtacgttt cttcggggcc
     4681 ggtgctgcca gcatagccac ggccaggatc ctcataagcg ccggggtcag gccggagaac
     4741 gttatcatgg ccgacagcaa ggggacgctc cacaagggcc ggaccgagct gaaggacgtc
     4801 ccctacgacg tgaagtggca ctttgcccag accaccaacc ccgagggtat cgaaggcggc
     4861 ccggaggtcc tcttcaggga tgctgatgtg gtctactgct acaccaaacc gggtcccgac
     4921 gtggtcaaga aggagtgggt cgcgaccatg gcaaaggatg ccatgctctt tgcctgcgcc
     4981 aaccccatcc cggagatatg gccctgggat gcgaaagaag cgggagcgag gatagttgcc
     5041 acgggaaggt ctgacttccc gaaccagata aacaattccc tcggcttccc ggggatcttc
     5101 cggggcaccc tcgacgtgca ggcctcgacc attacggacg agatgtgcat agccgcagcg
     5161 aagacccttt caaagtgtgc ggagaagaat ggcctctccg aagactacct tatcccgacc
     5221 atggatcagg cctgggtcta ccccgaggtg gcgaccgccg tcggcatgaa ggccatcgag
     5281 caggggatcg ccagaaaggt cctctccgga gaggaactcc tcaagacagc caccgaaaag
     5341 atccagtacg cccagaaaat gaccgcatgc ctcaccgaga aaaagttcat agaactcccg
     5401 taggacctgt ccctggtctt accaggtcca aaatcttcct gaaggggggc ttccggcaaa
     5461 ggggggcccc cctttcacgt gataccttcc aagagttata gtgtacagga gaaccgggga
     5521 tccaggcgct ctgttcggag ggctacaaac aggagaggag agatcgggct gctgccttta
     5581 tcctgtgtac tcgacaaacc ttacgagttc aatggcacct tgggaaagtc tttcaaaatg
     5641 gtactgtgag aagcaggaat acaaccgaag aatttcttca taaaaaagac ctgtgttaag
     5701 gggagtgaga tccatcagaa ggagatctct ggttattctt gccgttctgg cggcgctggt
     5761 ccttgtcttt gccgaaagag ggttttgggg accatcagcg atcagggttc cggaacaggt
     5821 ctctgcaggt gcagagctat tggagagcct ttatcgggaa aggaagggtg gagtccaggc
     5881 agaggctgga gggatcgtta cgaggctgct tcctgacgac gacaggggga gcaggcacca
     5941 gcgcttcgtg gtggaactcc cgaccgggca gaccctgctg atagcccaca acatagacct
     6001 ggctccccgg atcgacggcc tcaggaccgg tgaccggata gagttcagtg gcgaatacga
     6061 gtggaatccc gaaggagggg ttatccactg gacccatcga gacccctcgg gaaagcacag
     6121 gccggggtgg ctcgtccacg aagggcagaa atactggtga tcctttctat acttttctct
     6181 cttccaggac ggcccccaga aaaccggcgg cgcttgaaag catgaaccgc atctcgcgag
     6241 accttacatc cgccggaaga agcgaaaagt cggcaagcca tggatccctt tctacaacga
     6301 agccgagcag ttcccccatt tccacctggt tctcaaattt atatcccttc atgttgagga
     6361 actcactgaa aagctttctc catagacgga tctgctctcc cgcatggtcc agcatttccc
     6421 tgacctgctc cgtgcaaccg aagtgagcgt agcatagcaa ggacgcatca atgcctttca
     6481 gttcctcgat cgatcgcagc gcagtttcga tgtaaaaacg gggcggggat gccggcctga
     6541 gcatgtacct tctttcgccc gctcctggga ccaggtggtc aaggcgtgta aaggtgctcg
     6601 ccgcttcacc ggcaaagaga acctgacctt ccggggtcca gtagaaaaag gagcggtggt
     6661 gaggagcgtg accaggagtg tccacggcgg ttatgccagg gacagggacc tcttcgggga
     6721 taaagcagct ctcgtctacg ggagagggtt caccgtaggc cagggccatc tccggtatga
     6781 cctgcaggct gctctcccag agcctttcgg ggtttaccag gtgacgcttt cccttcgggt
     6841 ggacggccac cctcgccgaa gggaaggccc ggagcagttc ggcaagccct ccggagtggt
     6901 ccaggtggat atgggtaagg aggatatagt ccagtttttt aacatcaagt gtgtggagct
     6961 cttcgataag gatcgaagcc gttgatgtgg ggcccacatc aacaagggta ttccgttctc
     7021 ttccttcgtc ccggacaagc cagatcccga agaagtcgtg gtagccatcc aaatccaggg
     7081 ggaggtcgat aaggctcaca gaaacacccg gtgttatctt caggtcaagg atcttcactg
     7141 tcattcacct caatagttga tagcagtttc aggttccgac ccagagcatg gaacattatc
     7201 ccggagtggc gccaatctaa caacagctcc tgttcaccga aggcttgaca atcgatgtta
     7261 agtgggctac gttttggtat atcggttaca tttcagttga ataatggttg tataccagta
     7321 aggggatgcg atccttgaga gagagaacga agaccgattc ggtttacgag gagataaaga
     7381 agttgatcct caggggggag ctatccagtg acacaccgat ttcggagaac tacctcgccc
     7441 agaggttcgg aataagccgg acgcctatcc gtgaggctct gaagcgtctt gatatggagg
     7501 gtttcgtcag gatcataccc aaccagggtg tctttgtccg cgacgtttcg atcagcgagg
     7561 tcaaggaaat atatgacctg cggatagccc tggaagagtt tgttgtaaag gaacttaccc
     7621 acaccctgtc tgaagatgat ctctttgtgc tcgaggggaa ccttgcccgg caggaagaag
     7681 ccctccgtag acacgatgcc tttgctttcc atgaagagga ccgtaagttc cacgactact
     7741 tcatgaaggc ctacggtaat gacatgatca ctgacttcat aacgaacctg cgtgaccgta
     7801 tcgaggggat caacgtgaac atgctgaaac agccagggaa catggaactt ttttggggtg
     7861 agcacagaag gatactggaa gccctccgca gaagggacgg agagggggca accagagaga
     7921 tggatgagca cctgaagggc ggcaaggaga gacttctcag gggctgatcc gtagagaggt
     7981 acggcagtga ccgtttatta tcctgagagg agggataacg acaggggcag aggagaggtt
     8041 tccccaactg ggaggtaaaa aaacctgtga gggaggtgaa ctgtccggat ttcgccggac
     8101 aggacattaa gatgaggaaa ttgctgacag gaatactggt attggctttg ctcttcggtt
     8161 tggcaagcgt tgatgcagct tcagccgcac tcaagggtga gtacaagctc actatgaacg
     8221 taaccaccga tactgcctgg ggccagggcg gggtcaagtt cgccgaactg gtcaaggagt
     8281 acaccggagg caaggtgaac gtaaaggtct accccagcgc ccagcttgtc ggcggcgacc
     8341 agatgcgcca ggctgaaatg gtctccagtg gtgctataga cttcatgctc aactccacga
     8401 tcaacatcgc cccgatcatc ccggaatgca acgccttttc gctccccttc atgttcccca
     8461 gttacgagtc cgttgatgct gtgaccggct cggcagcggg ccagatggtc ttcgacgccc
     8521 ttgagaagca caacatggtc ggactggcat ggggcgagaa cggtttccgt gagctcacca
     8581 acaatgtcaa gcccatagct caccccgatg acatgaaggg tctcaagatc agggtcgtaa
     8641 cacctatata catcgatatc atgagagccc tcggcgcgaa cgccatcgcc atgaactggg
     8701 gcgaggtatt tacctcgctg cagcagggcg ttatcgacgg gcaggagaac cccattgtcg
     8761 ggatcataat cccgaacaag atatacgaag tccagaagca cctcaccaac tggcactatt
     8821 cctacgacgc ccttatcctg gccgttaaca agaacgtttg ggagagcttc gatccagata
     8881 tcaaggagca gatcaagaag gccgccgtcg atgccatgca ctggcaggtg cacgacgtga
     8941 gccgtgtagg cctgggagat ggcatcgagt tcctcaaatc ccaggggatg acagtgaccg
     9001 atcctactcc ggagcagctt gccgcgttcc gcgaaatgac caaggcggtc tatgagaagt
     9061 ggtccggaaa tgtcggtgcc gatatcgtaa aagccttcga ggaggctgtc tccagcttta
     9121 aataacatac catgctctag ggggagccgc aggagcggct cccctgttcc cgtcccggga
     9181 gaggcatctg tcggggatca tccgatagga agggaacggt gctaaggatg ttcaagttac
     9241 ttgacaggat cgaagagtgg acctgtgtca caatgctttt cttcatggcc atgctggcct
     9301 ttgtcaatgt gatcgtaagg tacctgactg ttttctcttt ctccttctcg gaggagctgc
     9361 ttgtgaacct cttcgtctgg ataacgatgc tcggagcttc gatcgcgacc cggaaggggt
     9421 cactgatcgg cgtcagttat ctctttgacc ggatgccccc gtcgctgaag aagttatcga
     9481 ccgcggtatt cgtcctctgc ggagtcaccc ttttcggcct tctcaccatg catgggatcg
     9541 atatggtcaa gagcgagttc aggtccggaa tgaccacgta ctccatggcg ctgcctatgt
     9601 ggatcttcgg aatggccgtg cctgtctgtg cctttatcct gatcctgaga acgatccaga
     9661 ggggcgtaca tgaatggaat gacctgtctg gtccacagac ggggtcctgc ccggaggtgg
     9721 accaaagatg agcatagatc cgggtgtttt tctgttaggc cttttcttta tcttccttgc
     9781 cttcaaggta cccatagccg tcgctctcgg catggcgggg atggccgtcg tctactggta
     9841 caacctggga gcacagatgt tcgctcccgt gttctttgcc aacatctcca aattctcgct
     9901 actggccatt ccattcttca tcctcgccgg ggtcatcatg gggagggcga agatctcaga
     9961 caagatcatc aggctgatca tgttaatggt gggacccgtg agcggaggaa tggcaattgt
    10021 gacgataatc acggccctct tctggggagc cgtttcaggt tcggggccgg ctaccgtcgc
    10081 cgcccttggc accattctga tcccgggcat ggtcaaggcc ggttacgaca ggggtttcgc
    10141 tgccgccctg acctcatcgg caagcggatt ggccatagtg atccctccga gcatcggctt
    10201 tatcgtctac ggtgttatca cgggagcctc gatcagcgaa ctgttccttg cagggataat
    10261 tcccgggatc ctggtgggtc tcttccttat agtcgctgcc tttatcatct ccgtagcgcg
    10321 aggctacaag ggcgacaggt tcggaacggc aaaggaggtc ctaacggcct tcaaggatgc
    10381 cttctgggct ctgctcactc cagtgataat cctggggggg atatatgggg ggatcttcac
    10441 tcccacggaa gccgctgctg ttggagtggc ctacggcctt ttcgtaggct ttgtcgtcta
    10501 ccgtacgctc accctcaagg atctttacga actccttgtg gacgcctgcg cttcttcagc
    10561 ggtggtgatg ctggtagtcg ccttcgccgg gatcttcgga tgggccatga tggtcctcgg
    10621 ggttgttgag agggtctccg agggagtcat agccatgagt tccaacccct tcgtggtgat
    10681 agccctgatc aacgtaatac tgatcatatc ggggatgcta ctggacgcca tatctatcta
    10741 ttaccttctg atgccgattt tcatgcccgt catcgcccat ttcggttggg acccggtctg
    10801 gttcggggtg gttatgacca tcaaccttgc cataggccag gtaacgcccc ccgtggcggt
    10861 caacctgtac gttggctgca atgtggcggg ggtctccatg gacgaaatat ccagggcggt
    10921 ggttcccttc cttctggctt ctatcgcggc cctggcggtg atcgtctacc tgccgggcat
    10981 taccctttgg ctgccgaacc tgctgatgcg ttagtgaatg tcccgaacaa caagatcata
    11041 cacggcaaca ggaccggatg atcattttca ggaagagggg tgggggatcc ttgagcgcaa
    11101 agaaagttaa ggagaagaag ggcctgaaca agtaccccag gacgcttctc gagcagttct
    11161 acaggaccat gcttacaata aggtctttcg aaaaaaaggt ggaggaaaag ttcctggccg
    11221 gcgagatacc gggtttcgtt catctttaca tcggcgagga ggccatcgca gcaggggtga
    11281 tctcgaacct ctccagggag gattatatcc agagcactca ccgcggtcac ggacacacca
    11341 tagccaaagg tgccgatctg aagagaatga tggccgagat cttcggaaag aaggaaggat
    11401 actgcaaggg gaagggaggc tccatgcata ttgccgatct gagcatcggg atgctgggag
    11461 ccaacggcgt tgtcggaggc ggtttcaata tcgccaccgg cgcagctctg gccacaaaga
    11521 tgcagggcaa gaacaacgta tcggtggtct ttttcggtga cggggcctcc aatcgcggta
    11581 ccttccatga ggccgcgaac atggccgcag tctgggacct gcccgtactg ttcgtttgtg
    11641 agaacaacca gtatgcctct accactccct acatgaccac tacctccgtc gagaacattg
    11701 ccgacagggc agcgggctac gggatgcccg gggtaatagt ggacgggaac gacatatttg
    11761 cagtatacga agagtcgcaa aagctgatcg accgggcgag aaggggtgaa ggcccgtcgc
    11821 ttctggagtg caagacctac cgggtaaagg gacactatgt gggagatccg gagcgataca
    11881 ggacaaagga agaagttaac aaggtctgcg agagcaatga ccccatcgac aggttcgagt
    11941 gcaccgtcct ggacggcaag ctcttcagca gggaggagct tgatgcgata aaagcctcgg
    12001 ccggtaagga agtcgaaaag gctctcgagt tcgccaggaa tgccgaggag ccggaaattt
    12061 cggaactctt cgcgggcgtc tacgcgtaag gatgggttgg tgaaaggatc atgagagaga
    12121 tcacgttctc acaggccacg ctggaagcta tggacgagga gatgacccgt gatgaaaggg
    12181 ttttcgtcat gggtgaggat atcgcaaggc agggtgggat cttcggacag ttcaaggggc
    12241 ttcccgacaa gttcgggaag gacagggtga aggacactcc cataagtgag acggccatcg
    12301 tcggagcggc cataggagct gccctgacgg gcatgagacc tgtagcggac atgcactttg
    12361 ccgatttcat gggcgtctgc atggacgaga tattcaacca gatggcgaag atacactata
    12421 tgttcggagg ccagaggtct gtccctatgg tgctgagggc tcctgacgga cttatcaacc
    12481 aggcagcggc acaacactcc cagagcatag aggcctggtt catgcacatc ccggggctga
    12541 aggtggtcat accctccaac cctgccgatg cgaaggggct tctgaaatct gccataaggg
    12601 acgatagtcc agtgatctac ttcgagcaca aggccctttt caacacccgc ggagctgtcc
    12661 ctgacggcga ggtgttgtcc cctataggca aggccaaggt ggcaagggag ggtagcgatg
    12721 tgacccttgt atcctactct cactgcatgg gtctttccct gaaggcggcg gatatccttg
    12781 ccggcgaaga tgtatcggtg gagtgcatag acctcaggac catctctcct atcgacaagg
    12841 aaaccattct tgcctcggtg gccaagacca ccaggcttgt tattgcccac gaagcggtta
    12901 aacagggagg tgtgggagcc gagatatcgg ccatagtagc cgaagagggg atcgacttcc
    12961 tcgacgcccc cattgttagg gtaggagccc cctttactcc ggtgcccttt gcccggcctc
    13021 tagaacaggc ctacagggtc acccccgaga agatcgccga agcgatccgg ggactcttct
    13081 gagaggactc ccggtaatga catgtgagga taaccgatgg aacaggtgac catgcccaag
    13141 ctcggcctga ccatgacgga gggtaccgtt tcgaagtgga aggtctcccc tggtgacagg
    13201 ataaaaaagg aggaagtgct cctggtcgtt gccaccgaca agctgaccta cgatgtgacg
    13261 tcacctatcg atggtgtcgt acttgagata ctggtcaaag agggcgagag tgttcccgtt
    13321 ggcaaagtcc tggcggtcct gggagaggcg ggagaaaagg tatctgccgc ggatcggcaa
    13381 ggggagaggc ccgaacctgg gccagaagag gcagtggagc gaccctcaac ctcgatacct
    13441 ctgtccagag gagttggaac gaatgaggat gttcccgggc cagaaagcaa ggggcagaca
    13501 gctgcggcgt cacccctcgc aaggaagtgg gcaaggtctt ttgggctcga catcggcgct
    13561 atcgaaggta gcggtcccgg tggccgggta gtgaaggacg atgttatgaa ggcctcgaca
    13621 aggtcccgtg ctaccccgtt ggcgcggaag atcgcaaggg aggctggtat cagcgttcag
    13681 gagatatcag ggacaggccc gatgggtcga accaccgccg gagatgttaa ggcatacctt
    13741 gcttcacctc ccaaggagag gatcagggtc tcccctgtgg ccgctaggct ggcggaagac
    13801 ctggaagtac ctctggggac gatcaaggtg tccggcagga tcatgaaggg cgacgtgctc
    13861 gaggcagcct gtgcagcgaa agagagtaaa actgtgaata ggagggttcc tctcaccccg
    13921 atgcggaagg tcatcgccga gaggatgacc ttcagcgctt cgaccatccc gtcggtcttc
    13981 ttcaacatcg agaccgattt caccgagttc atcggtttca ggaaggcctt gaacgaggag
    14041 cttgaaggga gcggggtcaa ggtctccttc aacgatatcc ttatgaagat ctgcgccagg
    14101 gccctggtgg agatgcccat ggccaacgcc tcatacgatc ccgggcggaa cgagtacatc
    14161 ctgcacgacg atatcaacat cgggatagct gttgccgtag aaggtggatt gctggtcccg
    14221 aacgtcaaga aagtacagga aaaacccttg cccgtgatcg ccatggaatc gctggagctg
    14281 gtcaacaagg cgaggaagag caggcttctc ccggaagaga tgcagggggg gacctttacg
    14341 atcaccaacc tggggatgtt cgggatccat gatttcatcc ccatcatcaa cccacccgag
    14401 gcctgcatcc tggcagtgaa cgtggtcacc gaacgcccgg tctgcaggga cgggcaggtg
    14461 gtagtaagac ccatgtcaat gataggcctt acggccgatc acaggatcct cgatggagcc
    14521 gatgcagcca ggttccttgc ccgggtcagg gagctggtgg aaaaaccctg gatgaccctt
    14581 gtccaggagt ggcagacaga taaatggagc gtgaactgat ggcgactatg atcaccatgc
    14641 ccaagctggg gcttacgatg acggaaggtt ccgtcctgga atggaagaag aatgaggggg
    14701 ataccctgaa gaagggtgag gtcgttcttg tggtagcgac ggagaagctt acctacgaag
    14761 ttgaggcccc cgatgacggg ttgctcctca ggatagccgt caaggccggt gagaccgttc
    14821 cggtcggggc gatcctgggt gctatcggag agaaggggga agagccagat ctcggagtcg
    14881 ccgtagcaga agaggtccca acctcgaaag atgaggccgc ttcggaaaga gagcaagaac
    14941 ggcccactgg ttcattgaag ggtaccgaac ccgctccggc gaagggagac acaggtaaac
    15001 gggtggtggt cattggcggc ggccccgggg gatatgtagc tgccattaga gcagcccagc
    15061 tgggcggcaa tgtcaccctg gtggagaaag agaggctggg aggtacctgc gtaaacgtgg
    15121 gctgcatacc caccaaggcc cttcttcact cggcggaggt gaatcgtacg gttgaagatg
    15181 ccgccgaagc aggtttgaag gtgggttctt tggaagtcca atgggacgtc gtccaggagc
    15241 gcaaggacca ggtcgtgaag cagctctcac agggcattaa ggcgcttcta tcggctcacg
    15301 gcgttgagat catcactggg gaagcctcgt tcatggatga caggaacatc ctggtccggt
    15361 ccggggaagg tgacaggaag atcgagagtg atgccgtgat cgttgccact ggttcggagg
    15421 catcccttcc ccccatctcc ggcgtggagc tcggtggtgt agtaacaagc actggagccc
    15481 tctctttcga taagatccca tcgaggatgg tcatcatcgg tggcggtgtg ataggtgtag
    15541 aatttgcctc catatatgcc gatttcggat gcagcgtgac gatcatcgaa atgctgcccc
    15601 agatactgcc gggtgcagac ggtgaagctg tttccgccct caggaaggtt ctcgaggcaa
    15661 ggggtattac aattcacacc ggggcaaagg ttggatccat agaggcagcc gggtcatcgc
    15721 tggtcatttc tgccgatctt tccggcaggg aagagaagat cggagccgac aaggtcctcg
    15781 tggcggtggg gcgcaagcct ttcacggaag gattgaacct ggaggcggca ggaataagga
    15841 tcgagaggga caggatcagc gtcgacaagt ccatgcgcac aagtgtgccc catatcttcg
    15901 ccgtgggtga ctgctgcagc ccgatcatgc tggctcacgt ggcaatgatg gaaggagagg
    15961 tcgctgcggg caacattttc ggcctcgatg agaagatgga ctacagcact accccaggtt
    16021 gtgtctacac ctcaccggaa ctggcctggg caggccttac cgaggaagag gcgaagaaga
    16081 gaggtcataa ggtaagaaag ggtgtcttcc caatgatagg gaacgccaag agcctgatct
    16141 ccggggagac ccggggaatg gtaaagatag tcgccgacga gaagtacgga gagatcctgg
    16201 gtgtacacat agtcggcgcg agagccacgg acctcatatc cgaggcttcc ctcgccatca
    16261 ggctcgaagc caccctggac gaagtggccg aaacgatcca tggtcatccc acgttctgtg
    16321 aagctacgag agaagctgtc cttgccggtc ttgaaagggc aattcacagc ataccccggg
    16381 tctgagcagt gtcggtacct ggaatgggga ggggccaatg aaatatatcg agaaccggaa
    16441 gaccgacccc aggttcaacc ttgcccttga agaggtgctc tttcagtcct tagatgagaa
    16501 agaggagggc ttcttccttc tctggcagaa tgagcctacc gtagtggttg ggaggttcca
    16561 gaacaccctt gaagagatca acgtccccgt ggtcagggag aagggcatcc acgtggtacg
    16621 gcgtctgtcc ggtggaggtg ccgtctacca cgacctggga aatctgaact ttacctttat
    16681 cctcaggacc gaaaaccccc agaacctgga cttcaggaaa ttgactgaac cggtaatatc
    16741 ggccctctgg agcctgggcg tggacggagg cctggtcggc aggaatgacc tggtgatcca
    16801 gggcaagaag ttctcgggca atgcccagta catgcgtgag gggagactcc ttcaccacgg
    16861 caccatcctt tacgacagcg atatggacgt tctcgaagag gtgctccagg cagacccgga
    16921 caagttcaaa tccaaggggg tcaagtcggc aagaagccgt gtgaccaaca taaggtccca
    16981 cctcaaggga gaacctgata ttgaagtttt caggagggcg ctccttttca ctgtaaagga
    17041 gcggtggccc ggtcttgaga tctccccgcc ggacccatcg gtgcaggaaa aggccgcggc
    17101 cctcgcggag agcaagtacg ggacctggga ctggaactac ggggcctcac cggagttcga
    17161 ccttcggaag gaaagacgtt tcggcttcgg gaaggtggat gtcaggctca acgtccgaaa
    17221 gggaaggata acctcctgca ggatcttcgg ggactttttc ggcaacgggg atatcgccgg
    17281 ccttgagaat ggtcttgcgg gaacgcagtt ttccgagaag ggcctttcgg ggatgctgaa
    17341 acgggtagat gtgagccatt acatttcagg gatggacagc cgttcattca tggaactggt
    17401 cctggagtag ctccaggacc agttccgtaa cgttttgcat ggatcaatga tctgcaggta
    17461 atgtcgtctg tctcctcaaa ttcttcggcg gcgtggaagt tgcccccctg cggtccggcg
    17521 acgagagcgg ggatccacaa ccttcctccg gtatagcaga agtcatacac gccggaaaag
    17581 caactggact caggagcacg acccgttgct ctctccataa ccccggaaat cccagtcacc
    17641 ataggtcaag aaattgagag gttatctgag gtcagacctt agttggaggt cgtgatcttg
    17701 ctttttcttt ctacttttgc gacgggcaat accatgtcaa acattttgtt gcatgccatt
    17761 tgtagagtta aattgctata ttgacactca aaaaccgacc ctatactcaa aatccataaa
    17821 gggagtgtat cgttggtgaa gaaaagggtt attactgcgc cattggtctt atttgttctg
    17881 tcccccgtag catttgccgc agagcctatc aagaaaggtt atgttgcgaa tctgacgggc
    17941 gacgcgggga gtccacgaga agaatggcgc gatcatagca ccggagtaga tcgacgcggc
    18001 cggaggagca ctcggtcgtc cactggaact tgttgtctac gatgtgaagg ccaaggcaga
    18061 ggatgccatc agtgccgtca ggcgatcgat ccttgatgac cgggttcccc ctcttctcga
    18121 ggagaagacc gtcgtcccac tcttcttcgg ggatgcccgc agctgcgagg gctacgctgt
    18181 tggccacctg gatatgggtg cagaccctga tcagcagcag aaggtgatcc ctcactgcgt
    18241 cggcaacggc tgaattataa cagcatggag tatcggggag ttccccttga gcccacaagg
    18301 gaagtgacaa tacaggtata atagtaataa aataaaactt agaaggaatc cagtgaagat
    18361 ctgtcttcat ttcaaagacg agaggtatat aatcgccttg ttcctggttt tgggaaacaa
    18421 agacatcgca actgtccccg tcacctccta tgtcgaaaga aagggtgatg cactttgaac
    18481 agagccgggt cattcgttgt gttgaattgc gccctggcaa tactggtggc gttattctct
    18541 ctttcggtca tcaaaggagg cgccgcagag gttttggaag gtccattgac gataagcacc
    18601 tcctacaaga gccttctttc gacccctgat cagaaaggaa tgcttgacct cctggtaaag
    18661 gaggcattca gcaggatcgg tcttgaggcc gagatagtct tcaaccctac aggaaggtcc
    18721 ctctcggatg tcaacgaagg tcttgccgac ggagagttga acaggataga ggggatggag
    18781 aagaactttc ccaatcttgt aagggttccc gagcccaaca tggtgatgga tttcgtggct
    18841 ttttcgaaga aagagtacat tatcaatggg tgggaaagca tcagcgacct gaggataggg
    18901 atagtaaagg gttggaagat cctggaggag aacaccgccg gctttcccga tgtaaccctg
    18961 gtgcccaccg agacggagct cttcaatatg ctcgacaagg accgcatcga cgtcgccctt
    19021 tacgacagac tcactggtta cgagcagatc cacctgagag gtttcgaagg gatgtttcac
    19081 cttgagccac cgctggcgag cagggagatg tacctctatc tcaacaggga gcatgaaagc
    19141 cttgctgggc ctttagccca ggctctcaga agcatgaagg aggacgggac ctgggacagg
    19201 atcgtaaccg agacaacggc tcacctcgca ttcggcggga acgttgggga ttgacgttcg
    19261 gcagcggaga aagatgatga actggagacg ttccagtacc tggagattct tcctttacac
    19321 cctgtccggt tttgtcctcg tctctatcgt cgtgacgggt atccttggct attatctgtt
    19381 caggcatgaa tcgggtcacc tggagcggaa tgtggaacag gttgaggaga tctaccttcc
    19441 cttcctgatc ccggctcttt gggtgacgga ccacgagtcc cttcagagcc agatagaggg
    19501 gatcgtcaag ttcagctaca tcgaccgggt cgaggtcagg aacgatgaag gcgacgtctt
    19561 cgccgcaggg gccgacccgg aaccctccct ggaagtgata tcccacgacc tggtctattc
    19621 caaccgcggg caatacagga agatcggttc gatctccctc tttgttgaca ggaacgaact
    19681 gttctcccat atcttcgagt ggatggggtc gctcttcctt cttcagttgc tcctggccgt
    19741 ggccctctcg tcggtcatgg cattcgtcta tcacatggtg atcgggagac agctcttccg
    19801 ccttgccggc ttcgtcaaga gcgcagatct gaccgatcct ggaaaggtct tcaagcttga
    19861 acacccatcc aggcagggcg atgaactcca gctccttgtc gaccacatca acgagatgag
    19921 aggcagtgtc aggagtcata tcctggagat ccaggagtgg cgagacctgc tccagtacgt
    19981 gatccaacat gatcccaatg ccattatggt actcgacagg gacatgaggt tcgtcttcgt
    20041 gagcgagaga ctgctcaaga acagagagct cgaaagggag agggtgatag gaaggacctt
    20101 catggaggtc ttgcctgaac tgccggagaa gtggatcgca atgttccgcc gtgcgttgga
    20161 aggagtggag cagagctccg atctggatta cgtggcccgt cctgacggta gcatggacat
    20221 gatcagctgg tggtgccgtc catggtatga accggacggt tccatcggag gtacagtcct
    20281 ctactctgag gtgatcaccg aacgcaagca gatggagcgc tccctcttcc ttgaaaagga
    20341 gctcttcagg accaccctgc tgtccgtcgg tgacggcatc atctccaccg acgacgaggg
    20401 gaggatcctt attatgaacc gtgtggccga ggagctgacc gggtgggacc tgacagaggc
    20461 cagggagaag gacttcaagt cagtcttcca tattatcgac gagttttcta gagagagatc
    20521 tcccgacccg gtaaggcagg tcctggacag cggagaaccg gtcgagatgg aggagaactg
    20581 tatccttgtc tcgagagggg gccaggaaac ccccgtagag gacagtgcgg ccccgatcag
    20641 ggacaaggaa ggggagacca cgggagtggt tgtcgtcttc agggattgtt ccgagaagag
    20701 ggagaggcag agagagatag cgtacctcag ttaccatgac cagcttaccg gcctgtacaa
    20761 ccgccgcttt ttcgaggagg aactaagtcg tctggacgtt ccccgaaacc ttccgctgtc
    20821 gttggtcatg atagacgtca acggccttaa actctttaac gacgctttcg gtcacaccgc
    20881 aggagatgaa cttctgggaa aggttgccgg agtgctccgg gaggagtgtc gagccgacga
    20941 catcgttgcc aggataggag gggacgagtt catgatcctg ctgcccttga cggaccggga
    21001 ccatctggat ccccttatgg ccaggatcag cgatgccatg tcaagagagc gagttaagga
    21061 tcttcccatt tccgtgtccc ttggaggagc cacgaagacc gatgttgatg agagcgttga
    21121 gaaggtcttc gatatggccg aggaccagat gtatcgccgc aagatatctg aaaagagcag
    21181 ctatcatcac aggtcggtga acctgatagt ggaagggctt tactccaagt ctcccgaaga
    21241 aaaaggacac tccgaaaacg tcagtgcgtt gagcgaggcc ataggccggg agatgggact
    21301 gggacagccg gatttagacg atctcaaaac tgccggtgtg atccacgata taggcaagat
    21361 cggagtaagc gacaggatcc tggaaaaaag cggtcccctg gatgaagatg aatggcaaga
    21421 gatgaaaagg caccccgaga tcgggtacag tatcctcagc acagtcaatg aatacagccc
    21481 cttcgcagag atcgtgcttt cccatcatga gcgatgggac ggtacgggct atcccaaggg
    21541 cctgaagaga ggggagatcc ctcttttctc gaggatcctg gcagtcgcgg aagcctatga
    21601 cgcaatggtc tcagggagat cctacaggaa agccatgggc agggaggaag ctgtggccga
    21661 actccgtcgt tgcgccggca cccagttcga tcccgaagtg gtagatgtgt tcatagaaaa
    21721 agtctttggc aagtgatggt caagaaaaag tcgacgagtg aaggatgcag gtgataggtc
    21781 tgaattaccc cactggatct gtcagaaaca aacaagcatc acaatcctat ccccttttcc
    21841 cgctcaacag gatctcctgt aggcagatcc catactgcaa atgagatcaa aacagaggat
    21901 cttacactgt gacacaagag aaaggccctt tggcttcaga tgaacccttg cagggagttt
    21961 ccccattgct gtcgagggct gtctttgggt aggcgcatag gttcaaagtt tgaaaggagt
    22021 ttataaatgt cgcaaaacaa ccggttcgac cacaaactgg atctgcgaac tctgctaccc
    22081 ga
//
LOCUS       DAOTIQ010000030        20895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3053_length_20895_cov_6.491939, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000030 DAOTIQ010000000
VERSION     DAOTIQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_20895_cov_6.491939"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="P5541_05310"
     CDS             complement(<1..1302)
                     /locus_tag="P5541_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV71433.1"
                     /translation="MTFGPQTDTMGRDPVGKLLLRFSMPSIIGLLANALYNIVDRIFI
                     GRIVGAQGLTAVTVGFPYFGLGITLGILISVGASSLVSRSLGKGDRKMARQVLSNALT
                     MAIIAGLSLLFSGLVFGRDLLRLFGASDTVLPEALGYTRIVAAGMPFLLVAFVLNGLM
                     RAEGAPRWAMGTMLTGTITNIFLDWLFIARFGWGVNGAALGTSIAQIMAVCWVAFFYL
                     RFSTLGISKNLMVLKKEILTRVLAVGMSPGLLELSFVTLLVMLNRIMGSLGGDLAISA
                     VGIFFSLDTLFFLPALGIGDGAQPLIGYNYGAGNMDRVRRIIILAIASAALIFTLFWA
                     VAEIFPVFLVGLFSRGDAELTAIAVRGIRLGYLLLPLASLFIVTSYTFQALGKARATF
                     VLQVLRQVVLFFPMLILLPRVMGVDGVWLSFPFMDLTGFLLA"
     gene            complement(1327..1902)
                     /locus_tag="P5541_05315"
     CDS             complement(1327..1902)
                     /locus_tag="P5541_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRV71434.1"
                     /translation="MEELFALMIGFGLSAACGFRVFIPLLAMSLAAQTGNLELAEGFQ
                     WVATPTVTAALGIATFLEIGGYYVPWLDNMLDAAATPAAIIAGTFITASVMPEMTPFL
                     KWSIAAITGGSAAGVVQGATVLTRAASSFTTGGFGNPIVSTAEAGGSIIITALSLMIP
                     LVTLGLLVFLFIWAGRKIYRRFRQPTPSGSQ"
     gene            complement(1908..2885)
                     /locus_tag="P5541_05320"
     CDS             complement(1908..2885)
                     /locus_tag="P5541_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV71435.1"
                     /translation="MRKSKIEISEKTTLDLIDLPLQEAGVKDFFGIWLIRDSVSDFTA
                     IVDVGPASTVSLLIDELTELGVNRIDHILLSHIHLDHSGGLAQVLRAFPSSMVTVHPK
                     GRRHLTDPEKLWESSLQVIPDMAAAYGKPLPVDEKFFLDESEAIPGIKVLETPGHAPH
                     HLSFLYDDGEERVLFAGEAASTYNNLDDLIPGSGKGKYLLRPASPPRFYLESALESIE
                     KLRRQKATTLCYAHFGYTREVDRMLGEAKEQILLWQNIFLEYLSEHPGTEPGEDLGTL
                     VSFVIDKDPWLSEFSRLPEETGKREMGFLLSSAAGFLGAVYPQPATGVK"
     gene            3031..4056
                     /locus_tag="P5541_05325"
     CDS             3031..4056
                     /locus_tag="P5541_05325"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRV71436.1"
                     /translation="MSNVKDLDWSNLGFRYVKTDYRYISRWKDGKWDEGRLVEDNMIS
                     ISEASNVLHYGQSCFEGMKAYRRKDGRIQLFRPDQNAKRMQESCRRILMPEMPADRFV
                     DACMKVVRANESWVPPYGSGATLYLRPFMFGTGENIGVSPAPEYIFCVFCLPVGAYFK
                     GGLSPVNFIVSDYDRAAPRGTGAAKVGGNYAASLYPHSIAAGKGYADCVYLDPLTHTK
                     IEEVGAANFFGITRNNRLVTPKSESILPSVTKYSLLHVARHTLGMEAEERDVYIDRLE
                     EFTEAGACGTAAVITPIGGIEYRDHLHVFFSETEVGPVTKKLYETLTGMQFGDIDAPE
                     GWIQEVS"
     gene            4147..5349
                     /locus_tag="P5541_05330"
     CDS             4147..5349
                     /locus_tag="P5541_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71437.1"
                     /translation="MVKEQIVSEIAALLSEMQLPVETGEITDISVRTDFLDAGWSTGK
                     KKISYETFIFANEQDNTVYMYEKTTEVGKGLSFGGGAGSSFQSGKTLFRKVKNVQYGP
                     DGKAYEIKLDLGAIPKAAKETAKKHGWKFKTVLSKSKALYPEGYSSAVPAATFHPEIP
                     AGRPADAGPSSATVSGAAPEDSPKPRYNDPEKPLYAEPGGKTPSRLGGIVGLVGFVLL
                     GLFLLLLLFAGGAAITGWVLTVALFGGSFYLQRKISRKGLLLNLALLLVAGFLMMIVA
                     AGFSTTDLSSARIKNAQMTTGLEAPSMKPVDKVKAYSVDSPEMIVSAELRRAPQNTRL
                     RFVWTYTTGDIPITAYEMDSGTAGTDIYIYNNIHNGGRPWPQGEYRVDIFIDDREKPD
                     AQVDFEVK"
     gene            complement(5386..5892)
                     /gene="msrB"
                     /locus_tag="P5541_05335"
     CDS             complement(5386..5892)
                     /gene="msrB"
                     /locus_tag="P5541_05335"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRV71438.1"
                     /translation="MKRTFVLILAIALIAPLWITATSRGKNTVGEVPEVDIKVIRTEE
                     EWKDLLTSEQFRVTRKGGTEPAFSGQYYNNKEEGTYNCICCGNPLFSSGTKFDSGSGW
                     PSFYEPTSPQSVRTREDRSFGMKRVEVLCSRCDAHLGHVFNDGPPPTGLRYCINSVAL
                     EFEKTEKE"
     gene            6037..7158
                     /locus_tag="P5541_05340"
     CDS             6037..7158
                     /locus_tag="P5541_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV71439.1"
                     /translation="MKIEEFKLERTLARFQNEVEYDLSASGIYPMFIREILSPGEMEE
                     VYNDLHLKYVHTAGTEHLRKAVASFYDGMDPSSVFMTNGSAEALFLMAWGLIDPGDEV
                     AFMIPNFMLLSHVVESNGAVVHNFRLDPEKGWSLDLESLKKAVTPKTKMICVCNPNNP
                     TGTVLTRGQMQDIVDVASEAGAYLLSDEVYRGAEQTDEMTPSFWGMYDKIIVISGLSK
                     SFGLPGLRIGWVAGPREVAEKSWFYHDFLSTTVTTFSDHLATKALEPAMREKIFSRNR
                     GIVRQNLATFTEWVDSHAGVLSFTPPQAGCFAFVRYDLPVSSWDLVMDMVENNSVFVI
                     PGSCFGIENHLRINFGVDEKRLVEGLKRIDRTLSRFSGR"
     gene            complement(7500..8453)
                     /locus_tag="P5541_05345"
     CDS             complement(7500..8453)
                     /locus_tag="P5541_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRV71440.1"
                     /translation="MDISSVCIIGSGTMGSSIALNFAKSGLDVDLFDISDRALERGFS
                     LIQTALDTLVGNGLTVKADVPVILSRVRGATDLGAAAANTAFVIECAPENLEIKQRVF
                     RQLEAHFPNDTIFASNTSSLGPTRISSALSWRNRFVAANFWNPAHLLPLVEIMPGEDT
                     SEETVRFTREIMERIGKEPVVLSRETPGYIGNRLQFALLREALYLVDSGIASKEDVDR
                     AVRFGIGRRLGDTGPLETADLGGTHVFAAICETLFKELCNSTEVSPTLLDAARKGDLG
                     YSTGKGIYEWPQEKLEKIQKRRQRSLIEWLRKDMESSGENI"
     gene            8585..9286
                     /locus_tag="P5541_05350"
     CDS             8585..9286
                     /locus_tag="P5541_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HRV71441.1"
                     /translation="MARSPMAMVLSVPLLVLVLLLAALPAFSATPNERITQSVEVIRD
                     MSSQDDVETMADLVKSAKGVVIIPRYVKAAFGIGGAYGEGLILRRDAAKGAWYGPSFL
                     NITGASFGLQIGVQSTALLLVITNDRGMERFYGDKFKLGADIDIAAGPVGRSAGAGTD
                     INLKASIYSYSMSKGLFAGLSLSGALMSSDDEANTSYWGSRYNPRDILNKRATTGQIQ
                     PLLSVLNDLIKKSGR"
     gene            complement(9320..11497)
                     /locus_tag="P5541_05355"
     CDS             complement(9320..11497)
                     /locus_tag="P5541_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRV71442.1"
                     /translation="MRLVIPLEELSDDRKGSGGGKAWALSRMIREGFPVPGGVVVPVE
                     AYRRYVDLTGAGEMIMMELNRKPFASMRWEEMWDAALRIRNLFLKTPIPPELELELAE
                     TFDRRFGVVPVAVRSSAPGEDSEGSSFAGLHESFVNIRGTRDIIENIRLVWASLWSDA
                     ALLYRKELGLDPAKSSMAVVIQELVEGERSGVAFSQNFTDPTTSLIESVWGLNQGLVD
                     GTVSPDRWLLDRKSGSVLKHTPAERKKAVRSSRGGTSLKKLPRALRDRPPLGPGEVDK
                     VYNLAMKAEKWFGKPQDVEWTLRGDSIFTLQSRPVTTGVKEDERSWNLSLRRSFENLK
                     ALRKRIEGDLIPSLIREAENLSKHSLSELEEIKLAQVLEERSAAVEKWTRVYWDEFIP
                     FAHGARLFGQIYNDRIKPEDPFSFIDILRPEKMAGTERNSRLEKIAKDIRQNRELKEE
                     LEKIESGLPSVVGEVFIRKAVKELGSDSALILPGREQEMALARLLLEISGRTAPQKTR
                     PGKPKDLEGKFLSSFQDEERQLAVDLLDLARASYRLRDEDNIYLGRVERLRDEALAEG
                     MARLSAREGAILERYSATEVIKALKDTNYVPSPEIGDKPSESEERSFRVSPRQITGQP
                     AGQGLAKGKARVIRGKNDLFSFRSGEILVCDAIDPNMTFVVPLASGIVERRGGMLVHG
                     AIIAREYGLACVTGVPDATELIETGDELTVDGYLGIVVIHRKD"
     gene            complement(11494..11943)
                     /locus_tag="P5541_05360"
     CDS             complement(11494..11943)
                     /locus_tag="P5541_05360"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV71443.1"
                     /translation="MKDADLFVEITKILRSQKLGVLGTCGTAGPFCSLVGFSFEKDLS
                     HLFFSTPRSTRKYANLAECGKAALLVDNRMNRPEDLHDAQAVTVTGTVRELKDPERER
                     AEKIHIERHPYLAGFSSSPTTAIFSLEVDEYSLVSRFQNVMIMRVKR"
     gene            12097..13257
                     /locus_tag="P5541_05365"
     CDS             12097..13257
                     /locus_tag="P5541_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HRV71444.1"
                     /translation="MGIDGFLEILGGNPVFWASVAVATGVLLGGVKIRGVGLGTSGAL
                     FTGLALGWLGATVPEEYFIWNLLVFVTAVGLLSSRDIGVVIRRWGFRFMLLAVAVTGA
                     GALATWLMALLFSGAVEPLLVGGTYTGALTSSPGLGAALEATGGNSLVTIGYTIAYPF
                     GVIAVVLAVQMVPAMLKVDIPKEREDLIKIIHEHEPSSAREIDPSAPFTLISFVICII
                     GGIVLGSLRIPIPWVGEFSLGSTGGALLLALFLGARGRIGPFPMGLDVKVLSALRLVS
                     LAYFLAVMGIMAGPDIPAILARHGVALVGIGIVSAMVSLVTGFVLGRCVFRLNWVLLA
                     GAIPGAMTSTPGLGAAIESTGCDECSAGYGATYPAAIFCMVLFTKMIMLALS"
     gene            13274..14335
                     /locus_tag="P5541_05370"
     CDS             13274..14335
                     /locus_tag="P5541_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71445.1"
                     /translation="MRIDERDTIFSRMAIPEGSDFYEEYYSAHPDKRETDDMLRSLHA
                     LGSPDTPTWDPAGSALTDSIFRLLSDMRPLSEGEPSGDPVETDPGRLTRWVLGAASYL
                     GADDVGVAAVEPDFYYSRRGRRAEVWGEPVEEAHPYGVAIISAMDPSMIHKGPLAPVM
                     AESAKGYLRSGVTAIALACAIREMGYRARAHTDGNYLVIAPRVAFKAGLGVFGRSGLL
                     IHRSFGPCARISVVTTDMPLVPGHPDPLVEAVRQFCSICGRCSNHCPGRAIPGGPADP
                     GAAVPWSLDAEKCYGAWRRMGTDCGVCISTCPFTAGMDWDEMNSAKDDPSRLESILER
                     TGGRELPRPFDPDPPGWWT"
     gene            14490..15725
                     /locus_tag="P5541_05375"
     CDS             14490..15725
                     /locus_tag="P5541_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71446.1"
                     /translation="MPRSFRFVSIILLLAFMVSSGAGVVESVTFESKSTDGTQPATQP
                     KVVGGSAKSGNSQEGALLYSLEAPQAQKDRLADAINGVHLTGIQATMAIEELKALENQ
                     NLPDNEILARLVYTEECFDALEKAAKKLNDSVELVKIACERDPELKGQVVAMLEGIFA
                     SPAYAFTGNPMADSYIAQEVQGIGRAAKKSFNSFMNTLSSAASTIGNAASKVKNAVGS
                     GVSALTGAVGKVHTKIGNVVGQKNWATIMAGTKFACTYVGGTVALIVAAPVTTAGAVG
                     AVVVWTAVNVGAGVSLANDLSQIQGGRGADGLDNALTRINQGTAIIGIAGGGSPGEVF
                     VNVMGVAGNEIVGTTPWREMTVEEIASHLEPKERQEFLKNIAALKKYQKPDALPTGGD
                     GGGGGGSSGGSCGCGGRND"
     gene            16136..17335
                     /locus_tag="P5541_05380"
     CDS             16136..17335
                     /locus_tag="P5541_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71447.1"
                     /translation="MSRAEKIVCLFLVFAFFFGSGAGIIESVTFESKPTDGSKSTTSS
                     PKVTGDTPSSKKPSGGSLLYTLDAPQSQKDRLAASIENLHSTSVQAAMALKNLKDLDG
                     KTVPTEEMLARLVHAQECFAVVETAAKKLEGDVELVRMACEKDPSLKQEVMAMLEGLF
                     ASPAMAAYAPPASVNSGSKSRWGSFMDSVKSAARTVGNVAKTAGNAIADGVKSVVGAV
                     GSAHDKIGSVVGQKTWATIMSGVKFTATVAGGVVAICAIPATATVGVAVAGAVIFGAT
                     YVGASVSLANDLMAIHGDQNIDGLDNAITRINQGAGAISLGVGLATGDVTKAGEVVAG
                     VMGIGANEIVNSLPGAEDMTDEKANEFLPPEHQIRVYKKPTSTGGSGGGDGGGGGGSS
                     GGCGGCP"
     gene            17400..18287
                     /locus_tag="P5541_05385"
     CDS             17400..18287
                     /locus_tag="P5541_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71448.1"
                     /translation="MRRFSLLFALVSIFCLSFFSVMPSMADETPPVAPALSFGEPETV
                     TVKDDTLMASINWTGDYIEATGQGLPPTGKENTPQGKLLARRAATVDLQRKLLEFVQG
                     VRVDSRTTMTDYMVSDRVRTEVQGMIKNVEILEGSWDGEVYTVVGRVKLRDVRIAVVP
                     ELPEKPVAKQVPPPPATTTAATPKGRYTGLVIDASHLPLIPAMTFNVTDTSGKPVYGL
                     SFVDKERFLSSGLCSYHMTVGYARIEPRVTDSPLVIKAVKLVGPHNVDIVISSSDAAR
                     IRNSTYDFRVPCRVIVVKR"
     gene            18294..19439
                     /locus_tag="P5541_05390"
     CDS             18294..19439
                     /locus_tag="P5541_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71449.1"
                     /translation="MNKSFKAFLAFLLLVLVSSVAFSGEAFSAEMISVQAEGAAAIVD
                     GNKDKARSEAKNQAYRDALEKGVGAMVQGITEMKDFQVVKDKVFSQSQGLVKTFDISR
                     EWEEDGIYYIEADCQVSAGALDGVLGPAVIDMLGNPRVMVLVDESIEGEKPFLSTVEG
                     EVLKVFEKAGYLLVDPGQSGALDQKELDLARETGDTARLQELAKTFRADVLIHGKAQG
                     LSFTQQKIEGVTLYGVRSQVQLKAVIAQTGYVLGTEMIEKKDKGTSPQDGAVKAFQAA
                     SGPVASTIVHKVAYALVSGQAGGIPGRTIKVVIEGVNFARLRDIKQEIESLDGVTSVY
                     QRSFASQKAELDVVAEKTSEGLAVDLERLGIEISSLSANTVEGKNIK"
     gene            19436..19906
                     /locus_tag="P5541_05395"
     CDS             19436..19906
                     /locus_tag="P5541_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71450.1"
                     /translation="MNRKGVCSVLVLLMTVSLFLPAPSFAISRNDLDTIWRNNGAVEG
                     VQEFRFGYVDWVGLSVSVDGKGQLRNNSAPARIMAQRAAVTDARRNLLLLLYEIRYGL
                     PARLESIDISGKVVEPHIDSESIVEDRYEIGITLPLERLLDECVIFSATVRQGE"
     gene            19914..20714
                     /locus_tag="P5541_05400"
     CDS             19914..20714
                     /locus_tag="P5541_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71451.1"
                     /translation="MKRQASILLASILILFIVAMQGSGDSALPKGKTIAVLVRSNSSQ
                     HARSAEAILINSLIQGGYKAVDRDRLESIRQNKAAALALEGDVDAILKLSQTYGFNVL
                     VSGRATIHTPVKNEFGLFTATAVVSVTACQGSDGRQLFADTSTAKEVGYTGDEAGQKA
                     LEAAARQAARAMTEGVPSGGGAASASRIFEVEVSGIRSFAEAHAVVEALSKAGCGSAN
                     LLRFAGGRAVVKASWGGNPDSLAAALSRQRGDISLERVEGDRILMVRR"
BASE COUNT         4756 a         5265 c         5915 g         4959 t
ORIGIN      
        1 ggcaagcaga aagcctgtca ggtccatgaa ggggaaggag agccatacac cgtcgacacc
       61 cataacgcgt ggaagaagga tcagcatcgg gaagaaaaga acgacctggc gaaggacctg
      121 gaggacaaag gtcgccctgg ccttgccaag ggcctggaag gtgtaactgg ttacgatgaa
      181 aagggaagcc aggggcaaaa gcaggtagcc cagccggatg ccccttacgg ctattgccgt
      241 gagctcggcg tcgcccctcg aaaagagccc gacaaggaac acagggaaaa tctctgccac
      301 tgcccagaaa agggtaaaga tcaaggcggc cgaagctatg gccagtatga ttatccttcg
      361 tacgcggtcc atattccctg ccccgtagtt ataaccgatc agcggctggg ccccgtcacc
      421 tatcccgagg gcgggcagga aaaaaagggt atccaggcta aaaaatattc cgacggcgga
      481 tatggcaaga tctccgccca gagagcccat tatccggtta agcatgacca ggagcgttac
      541 gaaagaaagt tccagtaacc ctggagacat tcctaccgcc agaacccgtg tcaatatttc
      601 tttcttaaga accatcagat ttttgctgat cccgagagtg ctgaaacgca agtagaagaa
      661 tgctacccag cataccgcca ttatctgggc tatggacgtg ccaagagcgg ccccgttaac
      721 cccccagcca aagcgggcta tgaaaagcca gtccaggaat atgttcgtaa ttgttcctgt
      781 gagcatcgtt cccattgccc atctcggagc cccttctgcc ctcatgagtc cattgagcac
      841 aaaagcaaca agaaggaagg gcatgcctgc ggccactatc ctggtgtaac ccagggcctc
      901 cgggaggacg gtgtcgcttg ccccgaaaag ccgcagaagg tccctgccga aaaccaatcc
      961 cgaaaacaac agagagagcc cggcaattat tgccattgtc aacgcgttgg agaggacttg
     1021 ccgtgccatt tttctgtccc ctttgccgag acttcgggat acgagggaag aagcaccaac
     1081 cgagatcagg atgccaagtg taatacccag gccaaagtag ggaaacccta cagtgacggc
     1141 cgtcaagccc tgagctccga ctattcggcc gatgaagatc ctgtcgacga tgttgtagag
     1201 tgcattggcc agaagtccta tgatggatgg catggaaaat cgcaaaagca actttccgac
     1261 ggggtcacgt cccattgtgt cggtttgcgg gccgaaagtc acatcaatat cctttccagg
     1321 gtcatctcat tgcgatccgg agggggtcgg ctgtctgaat cgcctgtaga tcttgcgtcc
     1381 tgcccaaata aagaggaaga caagcagccc cagtgtcaca agagggatca tcagggaaag
     1441 ggccgtgatt atgatggaac cgcctgcttc cgctgtggag actatcgggt ttccgaagcc
     1501 gcccgtggtg aaagaggagg ctgcccttgt caggacggtg gctccctgga caactcctgc
     1561 ggcgcttccg cctgttatcg ctgcgatgct ccatttcagg aaaggagtca tctcgggcat
     1621 taccgaagcg gttatgaatg ttccagcgat gatcgcggca ggggtcgcgg cggcatcgag
     1681 catattgtcg agccatggga cgtagtaacc cccgatttca agaaaagttg ctatgccaag
     1741 ggcagcggtt accgtgggtg tggctaccca ctggaaccct tcggccagtt caaggtttcc
     1801 cgtctgtgcc gcaaggctca ttgccagaag tgggatgaag accctgaaac cacaggccgc
     1861 gcttagcccg aaacctatca tcaatgcgaa cagttcttcc atggccgtta ttttacaccc
     1921 gtggcgggct gagggtaaac agcgccgagg aatccggcag cgcttgagag caaaaacccc
     1981 atttcccttt ttcctgtttc ctccggaagt ctggaaaact ccgagagcca ggggtctttg
     2041 tcgatcacga atgaaacaag tgttcccaga tcttcgccgg gttcggtccc ggggtgttcc
     2101 gagaggtatt ccagaaagat attttgccag agaaggatct gctcctttgc ttctcccagc
     2161 atcctgtcga cttcccgtgt atacccgaaa tgggcatagc acagggtcgt tgctttttgc
     2221 cgcctcagtt tttctatcga ttccagggca ctttcgaggt agaacctggg gggggatgct
     2281 ggccgcagga gatatttccc ttttccggat ccggggatca ggtcgtcgag gttgttatat
     2341 gtgcttgccg cctctccagc gaatagaacc ctttcctcgc cgtcatcgta gagaaaagaa
     2401 agatggtgtg gggcgtgtcc gggagtttcc agcaccttga taccaggtat tgcctcgctc
     2461 tcatcgagga aaaatttttc atccacgggc agaggtttgc cgtaggccgc agccatgtcg
     2521 gggatgacct gaaggctgct ttcccagagt ttttccgggt ctgtcaggtg acgcctgcct
     2581 ttcggatgga ctgttaccat ggaagaggga aatgcccgca atacctgggc aaggcctccg
     2641 gagtgatcca gatgtatgtg gctcagaaga atatggtcga tcctgtttac tcccagttct
     2701 gtcagttcat ctatcagcag tgaaaccgtg gatgctggtc caacatccac gatggcggta
     2761 aaatcactta cactgtcccg gatcagccat attccgaaaa aatcttttac tccagcttcc
     2821 tggaggggaa gatctataag atccagagtt gtcttttcgg agatttctat tttcgatttt
     2881 ctcatggttt tctccttttg cctgtttttt gagtctgttt cattacccgt cgcactataa
     2941 taaaataaat gtactgctat gcaatcccgg gtattggaaa ataaaagaag aaaggtctgt
     3001 tttccgaccg agtctggggg aggggaaaga atgagcaacg taaaagatct ggactggagc
     3061 aaccttgggt tcaggtatgt caaaactgac taccgctaca tatcaaggtg gaaggatggc
     3121 aaatgggacg aagggagact tgttgaagat aacatgatct ccatcagcga ggcttcgaac
     3181 gtccttcact acgggcagtc ctgcttcgaa ggtatgaagg cgtaccggag gaaagacggc
     3241 aggatccagc ttttccgccc cgaccagaat gccaaaagga tgcaggaaag ttgcagaagg
     3301 atacttatgc cggagatgcc tgcagacagg ttcgtagatg cctgtatgaa ggtggtcagg
     3361 gctaatgagt cctgggtccc tccctacggg agcggagcga ccctttacct gagacccttc
     3421 atgtttggta cgggcgagaa tatcggggtt tcccctgccc cggagtatat tttctgtgtt
     3481 ttctgtcttc ctgtcggggc gtatttcaag ggagggcttt ctcccgtcaa ttttatcgtt
     3541 tcggattatg acagggcggc acccagaggg accggggcgg cgaaggtcgg cggcaactat
     3601 gccgcgagtc tgtaccccca ttccattgcg gccgggaaag gttatgcgga ttgtgtttat
     3661 ctggatcccc tcacgcatac aaagatcgag gaggtcggag ccgccaactt ttttggaata
     3721 accaggaaca acaggcttgt gacccccaaa tccgagtcga tacttcccag tgtcacgaaa
     3781 tattcccttc ttcatgttgc caggcacact cttggtatgg aagcagagga gagagatgtc
     3841 tacatcgaca ggcttgagga gtttacggaa gccggagcat gtggtaccgc ggccgtgatc
     3901 actcccatag gagggatcga ataccgggat cacctgcatg tctttttcag cgagaccgag
     3961 gttggtccgg tgacgaaaaa gctttacgag acccttacgg ggatgcagtt tggagatatc
     4021 gacgctcccg aggggtggat acaggaagta tcctgatcgg acgggcaaaa gactggtgct
     4081 ttccaaaaca gttgctgaga aggaaaatgc tgtgaaggga ttccaacttt tggaggcggg
     4141 gtggtaatgg tgaaggagca gatcgtatcg gagatcgcgg ctctgttgtc cgaaatgcag
     4201 ttacccgttg agactggcga gataacggac atctctgtca ggaccgattt ccttgacgcc
     4261 ggatggagta cgggaaagaa aaagataagt tacgagacat tcatttttgc aaacgagcag
     4321 gataatacgg tctacatgta tgagaaaaca accgaagtag gcaagggcct ctcttttgga
     4381 ggtggggcag gttcctcttt tcagagtggc aagacgcttt ttcgcaaggt aaaaaacgtc
     4441 cagtacggcc cggacggaaa ggcctacgag ataaaacttg atctgggggc gataccgaag
     4501 gctgcaaagg agacggcaaa gaaacacgga tggaagttca agaccgtgct cagcaagtcc
     4561 aaggccctct atccggaggg gtactcttct gccgttcccg ccgccacctt ccatccggag
     4621 attcctgcag gcagacccgc tgacgccggt ccttcatcag ctacggtttc cggggccgcc
     4681 ccggaagata gcccgaaacc acgctacaac gatccggaga aaccgctcta cgccgaacct
     4741 gggggaaaaa caccttcaag attggggggc atcgttggcc tggtaggttt tgtgctgctg
     4801 gggctgttcc ttctgttgct cctctttgca ggcggagcgg ccatcacggg atgggtactt
     4861 accgttgccc tgtttggggg atcattttac ctccagcgca agatttcccg aaaaggcctg
     4921 cttctgaacc tggctctgct cctggtggca ggatttctga tgatgattgt tgctgccggt
     4981 ttttcaacca cagatctttc ctcggcccgt atcaagaatg cccagatgac cacggggctt
     5041 gaggcgccct caatgaaacc cgtagacaag gtaaaggcct attcagttga ttctccggag
     5101 atgatcgttt cagccgaact caggagagct ccgcaaaaca ccaggctccg tttcgtctgg
     5161 acttacacca caggggatat tcctattacc gcttacgaaa tggacagtgg caccgccggg
     5221 accgatatct atatctacaa caacatccac aacgggggga ggccatggcc gcagggagaa
     5281 tacagggtgg acatctttat cgatgacagg gaaaaacccg acgcgcaagt tgatttcgaa
     5341 gtgaaataga tgtttcccag gagggagggg ctgtggcccc tccctctatt ccttttcagt
     5401 cttctcaaac tccagagcga cggagtttat gcagtagcgc aggcctgtcg gtggaggtcc
     5461 atcgttgaat acgtggccga gatgtgcgtc gcacctggag catagaacct cgacccgttt
     5521 cattccgaag gatctgtcct cccgggtccg gacgctttgg ggtgatgtgg gttcgtagaa
     5581 actcggccac ccgcttccag agtcgaattt tgtcccggaa ctgaagaggg gattgccgca
     5641 acagatgcag ttataggttc cctcctcctt gttgttgtag tattgcccgg agaaggccgg
     5701 ttctgtaccc ccttttcgtg tgacacggaa ctgctcggag gtaaggagat ctttccactc
     5761 ttcttctgta cggatgactt ttatgtccac ttcgggaacc tccccaacgg tgttttttcc
     5821 cctggatgta gcggtgatcc agaggggagc aatcagtgct atcgcaagga tcaaaacaaa
     5881 ggttcttttc atgtggaatc accttctttt ttaaactgga atcattataa ttataccatt
     5941 ttatggcgac ccacaggtca aacccgttgt tgaaccggtt tgatgtgctg tagaatgcat
     6001 gcatgattta catctttccc tttacggagg cctgttatga agatagagga attcaaactc
     6061 gagaggaccc tggcaaggtt ccagaacgaa gtcgaatatg acctgagcgc ttcgggcata
     6121 tacccaatgt tcatcaggga gatactgagc ccgggtgaga tggaggaggt ttataacgac
     6181 cttcacctga aatatgtcca taccgccggg accgaacatc tgagaaaggc ggtagcctca
     6241 ttctacgatg gaatggatcc ctccagcgta ttcatgacga acggttccgc ggaagccctt
     6301 ttcctgatgg cctgggggct gatcgatcca ggggatgaag tcgctttcat gatacccaat
     6361 tttatgcttt tgtcccatgt cgttgagtcc aacggcgccg tggtacacaa ctttcgcctt
     6421 gacccagaga agggctggtc tcttgacctg gaaagcctca aaaaagctgt cactcccaaa
     6481 acaaagatga tctgtgtctg caatcccaac aatcctacgg gaacggtcct gacaaggggg
     6541 cagatgcagg atatcgtgga cgtagcctcc gaagccgggg cgtacctgct ctccgacgaa
     6601 gtgtacaggg gagcggagca gacagacgaa atgacgccca gtttctgggg gatgtatgac
     6661 aagataattg taataagcgg tctgtccaag tcctttggac ttcccgggtt aaggatcggt
     6721 tgggtggccg gtccccgtga ggtagcggaa aagtcatggt tttaccacga tttcctttct
     6781 actaccgtga ctaccttcag tgaccacctt gccacgaaag ccctcgaacc ggccatgcgg
     6841 gaaaaaatat tctcccgaaa caggggtatt gtcagacaga acctggcaac cttcaccgaa
     6901 tgggtcgaca gtcatgcggg ggttctttcc ttcaccccgc cccaggccgg atgcttcgcc
     6961 ttcgtaaggt acgatcttcc cgtctcttca tgggatctcg taatggatat ggtggaaaac
     7021 aacagtgttt tcgttatacc cggaagttgc ttcggtatcg agaaccacct gaggattaat
     7081 ttcggtgtcg acgaaaaacg tctcgtggag gggctgaaga ggattgacag gacactgtcc
     7141 aggttcagcg gaagataggc tttactgtgt ctaccttctg aggtgttctg atggttccct
     7201 ttactgcggg atagcccttg atccagtaaa gcgtctcagg cagcacaatg agagatgtgg
     7261 ttcaaaatat actgcctctc gccttccggt agagatcctt ttcgctacct cctgtcgttt
     7321 tacccgttcc gaggctcagg cactggagtc ggctgcaagg aaaagaaagc gattggacaa
     7381 ggtcaggttt ctggaggaga cagaaaggca tttttcgtaa aggattccaa aataatcggg
     7441 ggcgaggttg tctcttcgcc tccaatcatg atatcccggg tccatggatc cagggttttt
     7501 tatatgtttt ccccggaact ttccatatcc ttgcgaagcc actcgatcag tgacctctgt
     7561 cttcttttct ggattttttc aagtttttcc tggggccatt catatattcc cttcccggtg
     7621 gaatatccca ggtctccctt tctggcggca tcaagaaggg tcggggatac ttccgttgaa
     7681 ttgcagagtt ccttgaagag ggtttcgcag atcgccgcaa aaacatgggt tcctccaaga
     7741 tcagcggttt cgagggggcc ggtgtcgccc agccttcttc ctattccaaa cctgacggcc
     7801 ctgtctacat cctcttttga tgctataccg gaatcgacaa ggtaaagtgc ctcacgcaaa
     7861 agggcgaact gaagcctgtt gccaatgtaa cccggagttt ccctcgaaag caccacgggt
     7921 tccttgccta ttctttccat aatttccctg gtgaaccgga ctgtctcctc tgaagtatct
     7981 tctcccggca ttatctcgac gagcggaagg aggtgcgcag ggttccagaa attggcagca
     8041 acgaacctgt tcctccatga aagtgcggaa gatatccttg tcggaccaag gctggacgtg
     8101 tttgaggcaa aaatcgtgtc attagggaaa tgtgcctcca gttgccgaaa gactctctgt
     8161 tttatctcaa ggttttcagg ggcacattct ataacaaatg ccgtgttggc cgccgctgct
     8221 ccaagatcgg ttgctcctct tactcttgag agtattacag gaacatccgc ctttactgtc
     8281 agcccgttgc cgacaagcgt atcaagcgcg gtctgtatga gggaaaaacc tctttccaaa
     8341 gcccggtccg atatgtcgaa aagatctaca tccaggccgc ttttggcgaa attaagagct
     8401 atagatgatc ccattgttcc ggatccgatt atgcagacgg aactgatgtc cattgccttc
     8461 aaaaccgatc actgcctttc ataggggaac ggagatacca gtattttatg cgataatgat
     8521 gacttgcgtg cattagagta taatcaagac agaggaaaag atcccagtta aggagatgtt
     8581 gacaatggca agatcaccca tggcaatggt gctctctgtt ccgcttctgg ttttggtgct
     8641 gcttctggcc gctctgcctg ctttttctgc gacccccaac gagagaataa cccaatccgt
     8701 tgaagttatc agggacatgt cctcccagga tgatgtcgag acgatggcgg acctggtcaa
     8761 atcagccaag ggtgtcgtaa ttataccccg gtacgtaaaa gcggcctttg gtatcggcgg
     8821 tgcctatggc gaaggcctca tactccgacg ggatgcagcc aaaggggcct ggtatggtcc
     8881 aagtttcctg aatattaccg gagcttcttt cggactccag ataggagtcc agtccacggc
     8941 tcttcttctg gtaataacaa atgacagggg tatggagagg ttttacgggg acaagttcaa
     9001 actgggtgcg gatatcgaca tagccgccgg cccggtgggg agaagcgctg gagcagggac
     9061 cgacatcaat ctcaaggcat cgatatacag ttactcaatg agcaagggcc tctttgccgg
     9121 cctgtccctc agcggggcgc ttatgagttc cgatgacgaa gccaatacct cctactgggg
     9181 ctcaaggtac aatcccaggg atatcctcaa caaacgtgcc acgacagggc agatccagcc
     9241 ccttctttca gttctgaacg acctgataaa gaaatccggc cggtgatcga caagggtggc
     9301 agccccggga ctgccaccct taatccttcc ggtggatgac tacaattccc aggtagccgt
     9361 ccactgtcag ttcgtccccg gtctctataa gttctgtggc atcgggaacc ccggtgacgc
     9421 aggcaagacc atactcgcgg gctattatgg ctccatggac cagcatcccg cccctgcgct
     9481 ccactatccc cgaagcgaga ggtacgacga atgtcatatt ggggtcgatg gcgtcgcaga
     9541 caagtatctc ccctgaacgg aaagaaaaaa ggtcattttt cccccttatt actcttgctt
     9601 tcccttttgc caggccctgt cccgcgggtt gccccgtaat ctgtctgggg gaaacccgga
     9661 aactcctttc ctctgattcg gaaggtttgt ccccaatttc cggggaaggt acataatttg
     9721 tgtctttaag agccttgata acttccgtcg cggagtagcg ttccaaaatg gccccttccc
     9781 gggccgaaag tcgcgccatc ccttcggcaa gagcctcgtc tcttaatctt tccacccgtc
     9841 ccaggtagat gttgtcctcg tccctcagcc ggtaactggc cctggccaga tcaagcagat
     9901 caaccgcaag ttggcgttct tcatcctgga aggatgacag gaattttccc tcgagatcct
     9961 ttggctttcc cggacgggtt ttctgtggtg cggtccttcc ggagatctcc aggagcagcc
    10021 gtgccagggc catttcctgc tcccttcccg gaaggattaa agccgagtca gaccccagtt
    10081 ccttcacggc tttcctgata aaaacttctc caacaacaga tggcagccct gactcgattt
    10141 tttccagttc ttccttcagt tccctgtttt gccttatgtc cttcgctatc ttttcaagac
    10201 gtgagttcct ctctgttccc gccatttttt cggggcgcag gatatcaata aaggagaagg
    10261 ggtcctccgg ttttatcctg tcgttgtata tctgcccgaa aaggcgggcg ccgtgggcaa
    10321 agggtatgaa ttcgtcccag tagactctgg tccatttttc cactgccgcg gacctttcct
    10381 ccaggacttg agcgagtttg atctcctcca gttccgaaag agaatgtttt gagaggtttt
    10441 ccgcttccct gatgagagag ggtataaggt cgccttctat ccttttcctc agggccttga
    10501 gattctcgaa acttcttcgg agtgacaggt tccaggacct ttcgtcttct ttgacccctg
    10561 tcgtgaccgg tctggactga agtgtgaaga tagagtcccc cctgagagtc cattcaacgt
    10621 cctggggttt cccgaaccat ttttcagctt tcatggcaag gttgtatacc ttgtcaacct
    10681 caccagggcc caacggcggc ctgtcgcgca aagccctggg cagtttcttc aggctcgtcc
    10741 cgccccgaga ggagcgaact gccttttttc tttccgccgg ggtgtgcttc aggaccgatc
    10801 cggattttct gtccagcagc cacctgtccg gggatactgt cccgtcgacc agaccctggt
    10861 tgagtcccca aacggactcg atcagcgagg ttgtagggtc cgtgaagttc tgcgagaaag
    10921 ccaccccgga cctttcgccc tccaccagtt cctggatcac tacggccatg gaactctttg
    10981 ccgggtcgag acctagttcc ttcctgtaaa gcagggcggc gtccgaccag agcgaggccc
    11041 agacaagccg gatattctcg atgatgtccc gggttccccg gatattcacg aaagattcgt
    11101 ggagtcccgc aaaggacgag ccctccgaat cctccccagg tgccgatgac cttacggcta
    11161 ctggtactac gccgaatcgc ctgtcgaaag tttcggccag ttccagttcc agttccgggg
    11221 ggatgggggt cttcaaaaac aggttcctga tcctcagggc cgcgtcccac atctcctccc
    11281 atctcatgga ggcaaagggt tttctgttca gttccatcat gatcatctct cctgcccccg
    11341 taaggtccac atacctccgg tatgcttcaa cgggaacgac cactcccccg ggaacgggga
    11401 aaccttccct gatcatccgg gaaagggccc aggcctttcc cccgccggag cctttgcggt
    11461 catcggagag ttcttcaagg ggtatcacca gtctcatcgc tttaccctca ttatcataac
    11521 gttctggaaa cggcttacga gggaatattc atctacttca agtgaaaaaa tggctgttgt
    11581 cggtgaggaa gagaaaccgg caaggtaagg gtgtctctcg atgtgtatct tttcggctct
    11641 ttcccgttcc ggatctttga gttcccttac ggttcctgtt acagtgacgg cttgtgcatc
    11701 atggagatcc tccggtctgt tcattctgtt gtccacaagc agggccgctt tgccgcattc
    11761 cgccaggttc gcatacttcc gggtcgacct gggtgtagag aagaaaagat ggctcaggtc
    11821 cttttcgaaa ctgaaaccca ccagactgca gaatggaccg gctgtgccgc aagttcccaa
    11881 aacccccagt ttctggcttc tgaggatttt ggttatctcc acgaaaaggt ccgcatcttt
    11941 catttttctg cccccttctg ttatccttcc ctgggtcaat cttataacct cgcgccaggc
    12001 caggcaaagg gtgctttccc ggacagtatc gtggctgtaa cattgggtgc gtggtcgatg
    12061 ggtcctgcgg gcgattttcc gtgtgggggt ataagaatgg gtattgacgg tttcctggag
    12121 atcctgggag gtaacccggt tttctgggcc agtgtggcgg tagcgaccgg ggttctcctc
    12181 ggtggagtaa agatccgggg tgtggggcta gggacatcgg gggccctttt tacgggtctc
    12241 gcgttggggt ggcttggggc aaccgtcccc gaggagtatt tcatctggaa tcttcttgtt
    12301 tttgtaaccg ccgttggcct gctctcgtca agggatatcg gagtggtgat aaggcgttgg
    12361 ggtttccgtt ttatgctttt ggctgtggcc gtcacggggg caggggccct tgcgacctgg
    12421 ttgatggccc tccttttttc cggagcagtg gagcctctgc tcgtaggggg gacctacacc
    12481 ggagccctta ccagttctcc aggccttgga gctgctctgg aagccacggg cggcaacagt
    12541 cttgtgacca taggctatac catagcctac cctttcgggg ttatcgcagt ggttcttgcg
    12601 gtgcaaatgg tacctgccat gctgaaggtg gatatcccaa aagaaaggga ggatctcatc
    12661 aaaataattc acgaacacga gccttcaagc gccagggaga tcgatccgtc tgctcccttt
    12721 acattgatat ctttcgtaat atgcattatc gggggcattg tcctgggttc tttaaggata
    12781 ccgataccct gggtggggga attttctctt ggaagcacgg gtggtgcgct gcttctcgcc
    12841 cttttcctgg gggcaagagg gcggataggc cccttcccga tgggtctgga tgtcaaggtt
    12901 ctttcggctc tccggcttgt ttctctcgca tatttcctgg cggtaatggg gattatggcg
    12961 ggacctgata tccccgcaat tctggcacgg catggcgtgg cccttgtggg aataggaatt
    13021 gtctcggcga tggtgagcct ggtgacgggt tttgtccttg ggcgatgtgt attcaggctc
    13081 aactgggtgc ttctggcggg ggctattcca ggagccatga ccagcacgcc ggggcttggg
    13141 gcggctatag agtctaccgg gtgcgacgaa tgttccgcgg gctacggggc cacttacccc
    13201 gcggccattt tttgtatggt cctttttacc aaaatgatca tgctcgctct ttcctgagag
    13261 aggagaacag gttatgcgga tcgatgaacg ggacacgatt ttttccagga tggcgatccc
    13321 tgagggttcg gatttttacg aggaatatta ttcggcccat cccgacaaga gggaaacgga
    13381 cgatatgctt cgttcgcttc atgctcttgg ctccccggat accccgacat gggacccggc
    13441 tgggtccgct ctcacagact ccatattcag gctgctttcc gatatgcgac ctctttctga
    13501 aggagaaccc tcaggggacc cggtcgaaac tgatcccggg agactgacca ggtgggtcct
    13561 tggtgccgcg tcctatctcg gggcggacga tgtcggggtc gcggctgttg agcctgattt
    13621 ttactactcc cgcaggggga gacgcgcgga agtctggggg gaacccgtag aggaggctca
    13681 tccttacggg gtagcaataa tttcggccat ggatccttcg atgatccaca aggggccttt
    13741 ggcaccggta atggccgaaa gcgccaaagg gtacctgaga tctggtgtca cggccattgc
    13801 actggcctgc gccatcaggg agatggggta ccgggcaagg gcccataccg acgggaacta
    13861 ccttgttata gcgccgcgcg tggcatttaa agccgggctc ggggtcttcg gcaggagcgg
    13921 cctgctgatc caccggagtt ttggtccctg cgccaggatc tctgttgtta cgacagatat
    13981 gcccctcgtg ccaggtcatc ctgatcccct ggtcgaggcc gtgagacagt tctgctcgat
    14041 atgcggcaga tgttcgaatc attgtcccgg aagggccatt cctggtggac ctgccgatcc
    14101 gggtgctgct gttccctggt ctctggacgc agaaaaatgc tacggtgcct ggaggagaat
    14161 gggcactgac tgcggggtat gcataagcac ctgtccattc acggcaggga tggactggga
    14221 cgaaatgaat tccgcaaaag acgatccatc aaggctcgaa agtattctgg aaagaaccgg
    14281 cggaagggag ttgccccgtc cctttgaccc tgatcccccc ggttggtgga catgagaaag
    14341 ggacaagcgg agcggaggtt tttgcaatat actcaactag ataaactact gaaagggtaa
    14401 acaaaagaaa ttttaacagg attttttctc tttggctctg gtataatgat ccttgacatt
    14461 tttttgttac cgaagggaga gaagagtaga tgccaaggtc tttcaggttt gtatcgatca
    14521 ttctgcttct ggcttttatg gtgagttccg gagccggggt ggtcgagtcg gtgacctttg
    14581 aaagcaagtc aaccgatggc acccaacccg ccacgcagcc caaggtagtg ggcggatctg
    14641 ccaagtcagg aaactctcag gagggtgccc tcctttactc tctggaagct ccgcaggccc
    14701 agaaggaccg gctggccgat gccattaacg gggtccatct tacggggatc caggcgacca
    14761 tggcgattga agaactcaaa gcgctggaga accagaacct gcccgacaac gagattctgg
    14821 cgaggctggt ttacacggaa gaatgtttcg acgcgctgga aaaggccgca aaaaagctga
    14881 acgacagcgt ggaactggtc aagatagcct gcgagaggga tcctgaactg aaaggacagg
    14941 tcgtggcaat gctggagggg atattcgcct ccccggctta tgcctttacc ggaaacccca
    15001 tggctgactc ctacattgcc caggaggtcc agggtatagg cagggcggca aaaaaaagtt
    15061 tcaactcctt catgaacacc ctttcaagcg ccgcctcgac cataggtaat gctgccagca
    15121 aagtcaagaa cgctgtgggc agtggtgtga gtgcccttac cggggctgtg ggaaaggtcc
    15181 atacgaagat aggtaacgtg gtaggtcaga agaactgggc taccataatg gccggtacca
    15241 agtttgcctg cacctacgtt gggggaacgg tcgcccttat cgttgccgca ccagttacaa
    15301 ccgcaggggc ggtaggagcc gtggtggtgt ggacggccgt caatgttgga gcgggggtct
    15361 cccttgccaa tgatctttca cagatccagg gtggtcgcgg agccgatggt ctcgacaacg
    15421 cactgacgag gatcaatcag ggaacggcta tcatcggtat tgccggaggg ggaagtcccg
    15481 gagaggtctt tgtcaacgtg atgggcgttg ccggaaacga gatcgtaggt actactccat
    15541 ggcgggagat gacagtcgaa gaaatcgcga gccaccttga gccgaaggaa cgccaggaat
    15601 tccttaaaaa tattgctgcc ctgaagaaat accagaagcc cgatgccctt cccaccggtg
    15661 gagatggtgg tggaggcggc gggtccagcg gtggaagctg cggttgcggg gggcgtaatg
    15721 actgatttgt tgttaataac cggaatgtcc gctttgagtt aaatttttct ctggaagcac
    15781 gagccgagaa atacggccga tggttcccag ttcgcacttc tcctggcaaa acggaggatt
    15841 gatttgatgc ggaaagggag gttccttttg aggcgtttca acctgctgtt tgcatcggga
    15901 agaatgcttg atttttccag cgcttcggtc tgatcttaat agggaatatg gctaaaaatc
    15961 aatttagtag tattggaggt gtcaggatat cttggagaaa aaattgatga aatgatttgg
    16021 tatggtaaaa agaatctgac aagaagccca attagacact gacaacacct tttaaactcc
    16081 tttaagataa ccatcagagg cccgttggtc ttttgggata aggagatgag aggaaatgtc
    16141 cagagctgag aaaatagttt gcctgtttct tgtttttgcg ttttttttcg gttcgggtgc
    16201 gggaatcatt gaatctgtca cctttgaatc aaaaccgaca gatgggagca aatcaacgac
    16261 ctcatcgcca aaagtgactg gtgatactcc atcttcaaaa aaaccctcag gtggttcgct
    16321 tctttacact ctggatgcgc cgcagtcaca gaaagaccgt cttgctgcat ccattgaaaa
    16381 cttgcatagt acgagtgtcc aggcggctat ggctcttaag aacctcaagg accttgatgg
    16441 taagaccgtc ccgacagagg agatgctggc aaggctagta cacgcgcagg agtgttttgc
    16501 cgtggtcgag acggccgcaa agaagcttga aggagatgtt gagcttgtaa gaatggcctg
    16561 cgagaaagat ccatcattga agcaggaagt catggcaatg cttgaaggtc tgttcgcttc
    16621 tcctgccatg gctgcttatg ccccaccggc ctcggtcaac agcggttcga aatcaaggtg
    16681 gggttctttc atggattctg tgaagagtgc agcaaggacg gtgggtaatg tcgccaaaac
    16741 agcgggtaat gccattgcgg atggtgtcaa atccgttgtt ggcgcggtgg gtagtgccca
    16801 tgacaagata gggagcgttg tcggacagaa gacttgggcg acaattatgt cgggggtaaa
    16861 attcaccgca accgtagcgg gcggagtcgt tgcgatctgc gcaatcccgg caactgcaac
    16921 agtcggtgtt gccgttgccg gtgcggtgat atttggggca acgtatgttg gtgcaagtgt
    16981 ttccctggca aacgatctga tggcaataca cggagaccaa aacattgacg gtcttgataa
    17041 tgccattacg aggatcaacc agggagctgg agctatcagt ttgggagttg gcctggccac
    17101 aggtgacgtc acaaaggctg gagaagtagt ggccggtgtg atgggtatag gcgcaaatga
    17161 gatcgtgaac tcacttcctg gagccgaaga catgactgat gaaaaagcaa acgaattcct
    17221 tcccccggag caccagatcc gtgtttacaa aaaaccgacc agcacaggtg gttctggagg
    17281 cggagatggt ggtggaggcg gcgggtccag cggtggttgt ggtggttgtc cctaggtttt
    17341 caggttttct ttggtttggg ataaacgtgg aggattgata cagaaagggg aggttccttt
    17401 tgagacgttt cagcctgctg tttgcattgg tgtcaatatt ttgcctgtcc tttttcagcg
    17461 tgatgccatc catggcggat gagactcctc ctgtcgcccc cgccctgtca ttcggagaac
    17521 cggaaacggt gacggtaaag gatgacacac tgatggcttc catcaactgg accggggact
    17581 acatagaggc gacaggacag ggactgccac ccacgggcaa ggagaacacg ccccagggga
    17641 aactccttgc gaggagggcg gcaacggtgg acctccagcg taagttgctt gagtttgtgc
    17701 agggtgtgcg cgttgactca aggaccacca tgacagatta catggtcagc gaccgggtga
    17761 gaactgaagt ccaggggatg ataaaaaacg ttgagatcct ggagggaagt tgggacggag
    17821 aggtatacac cgtggtcggc agggttaaac tgcgggatgt ccgtatagca gtcgtcccgg
    17881 aactcccgga aaaacccgtt gccaaacagg ttccaccccc ccctgccacg acgaccgcag
    17941 cgactcccaa ggggcgttac acgggactgg taattgatgc cagccacctg ccgctgatcc
    18001 cggccatgac cttcaatgtt accgatactt cgggcaagcc tgtctacggt ctttcattcg
    18061 tagacaagga gcgtttcctg agttccggac tctgctccta ccacatgacc gtaggatacg
    18121 ccaggatcga gccccgggtc accgattctc cgctggtcat aaaggctgtc aagctggtgg
    18181 ggccccacaa cgttgatatt gttatatcca gttccgatgc cgccaggatc agaaacagca
    18241 cctacgattt ccgtgttcct tgtcgcgtaa ttgttgtcaa gaggtagatg gtgatgaata
    18301 aatcattcaa ggcttttttg gccttccttc ttttggtgct ggtttcttcc gttgcttttt
    18361 caggtgaggc cttctccgcc gaaatgatca gtgtccaggc cgagggggct gcggcgatag
    18421 tggacgggaa caaggacaag gcgcgatccg aggcaaagaa ccaggcatac cgcgatgccc
    18481 ttgaaaaggg agtaggtgct atggttcagg gcattaccga aatgaaggat tttcaggtcg
    18541 tcaaggacaa ggtcttctca cagtcccagg gactggtcaa gactttcgat atttccaggg
    18601 aatgggaaga ggacgggatc tattacatag aggccgattg ccaggtctct gccggtgccc
    18661 ttgacggtgt actgggcccg gcagttattg atatgcttgg caatccccgg gtaatggttc
    18721 tggtcgatga atcgatcgaa ggggagaaac cattcctttc cactgttgaa ggggaagtcc
    18781 ttaaggtatt cgaaaaggcc ggatatcttc tcgtagaccc gggacagtcg ggagctctgg
    18841 atcagaagga acttgacctg gccagggaga caggggacac ggccaggctt caggaacttg
    18901 caaaaacctt cagggccgat gtcctcatcc atgggaaggc acagggactc tccttcacgc
    18961 agcagaagat agagggtgtt accctctacg gtgtccgttc ccaggtccag ttgaaggcag
    19021 tgatagccca gaccgggtat gtcctcggaa ccgaaatgat agaaaagaaa gacaagggga
    19081 catctcccca ggatggtgcg gtaaaggcat ttcaggcagc ctcgggaccg gtggcctcaa
    19141 caatcgttca taaagtggcc tacgcgctgg taagcggaca ggctggcggc attcccggcc
    19201 ggacgatcaa ggtcgtgata gagggggtca atttcgcaag gttgcgggac atcaaacagg
    19261 aaatcgagtc ccttgacggt gtcacgtcgg tctatcagcg cagtttcgcc agccagaagg
    19321 cagaacttga cgttgtcgcc gaaaaaacat cggaaggcct tgctgttgac ctcgagaggc
    19381 tgggtatcga gatatcatcc ctgtcggcta ataccgtcga agggaagaac ataaagtgaa
    19441 ccgcaagggg gtttgttccg tccttgttct tctgatgacg gtgagcctgt ttttacccgc
    19501 gccatccttt gccatatcac ggaacgacct ggataccatc tggagaaaca acggtgcagt
    19561 tgaaggagtc caggaatttc gtttcggata cgtggactgg gtaggtttgt ctgtttccgt
    19621 ggacgggaaa ggccaattac ggaacaactc cgcacccgca aggatcatgg ctcagagggc
    19681 agccgtcacg gatgcgagga ggaacctcct tttgcttctg tacgagattc gttacgggct
    19741 cccagccaga ctggagtcca ttgatatctc tggaaaagta gtagagcccc atatcgattc
    19801 cgagagtata gttgaagaca gatacgagat aggcattact ctgcccctcg agaggctcct
    19861 cgatgaatgc gttatcttca gtgccacggt aagacagggg gagtgagacg caattgaaga
    19921 gacaagcctc gatccttctt gcttcgatcc tgatactgtt tattgtcgct atgcagggct
    19981 ctggtgacag cgccctgccc aagggcaaga ccatagcggt acttgtgaga agtaattcgt
    20041 cacagcatgc caggtccgcc gaggccatcc ttataaacag tctcattcag ggtggttaca
    20101 aggctgtgga cagggaccgc ctcgagagca ttcgtcagaa caaggcggct gcccttgccc
    20161 ttgaaggaga tgttgacgcg atcctcaagc tcagccagac ctacggtttc aatgttctgg
    20221 tatctggaag agcaacgata catacccccg tcaagaacga gtttggcctt tttaccgcta
    20281 cagccgtggt ttcggtgaca gcctgccagg ggagcgatgg aaggcaactt tttgccgata
    20341 cgtccacagc caaggaggtc gggtacaccg gggatgaagc ggggcagaaa gccctggagg
    20401 cagcagccag acaggctgcc agagccatga cggaaggtgt cccctccgga ggaggagcag
    20461 cctctgcttc acggatcttc gaggtagagg tatcgggcat aaggtcattt gcggaggccc
    20521 atgccgtagt cgaggcgctg tcaaaggccg gctgcggaag tgcgaatctt ttgaggtttg
    20581 ccggggggcg agcggttgta aaggcttcct ggggaggtaa ccctgattct cttgcggccg
    20641 ccctctccag gcagagaggt gatatttccc ttgagagggt cgaaggggac aggatcctca
    20701 tggtaagacg ctgaagttta tagataattc cttaaggggg aattgacaat gaaaagatct
    20761 gggaggaatc tgagttttgc tttgctggcg ctggtgggga tcctgttttt tgtttttccc
    20821 ctcgtgaccg atgccgaagt gaggctcttt gttgacaaga acactgtcag ggtccatgat
    20881 cccgagcttg gccag
//
LOCUS       DAOTIQ010000031        20262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3199_length_20262_cov_7.740338, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000031 DAOTIQ010000000
VERSION     DAOTIQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 20262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20262
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_20262_cov_7.740338"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..1644
                     /gene="yedE"
                     /locus_tag="P5541_05405"
     CDS             547..1644
                     /gene="yedE"
                     /locus_tag="P5541_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HRV71452.1"
                     /translation="MFSSLNRLLMSRMGPAVTGAVVGILAPLLSFWGNPANMGICVAC
                     FSRDIAGALGMHRAAVVQYIRPELSGFILGSLAAALVYREFKPRAGSSPIIRFFLGMF
                     AMIGALVFLGCPWRAYIRLSAGDLTAIAGIVGLTVGVVIGIYFLWTGFSLGRSYQAKK
                     PMGMIMPALAVSLMALLILAPSFGPDGTGPIFFSEKGPGSQFAPIWIALGVGLIVGWL
                     AQRSRFCTVGAIRDLIMLKDGHLFKGVLAFIVAAFVTNFLLGQFNPGMEGQPVAHTNH
                     LWNFMGMVLSGLAFTLAGGCPGRQFIMSGEGDGDSGIFILGMIVGAGIAHTFSFASSP
                     AGPGPFGPGATIVGLIFCLAIGFLMREKVSV"
     gene            1657..1881
                     /locus_tag="P5541_05410"
     CDS             1657..1881
                     /locus_tag="P5541_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRV71453.1"
                     /translation="MSESTTVDARGLSCPQPVVETKKAMNAQPGGKIEVLVDTVTSRE
                     NVSRLARNQGWEVSAEDLPDGGFRVILTKA"
     assembly_gap    2319..2418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2597..3628
                     /gene="selD"
                     /locus_tag="P5541_05415"
     CDS             2597..3628
                     /gene="selD"
                     /locus_tag="P5541_05415"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2630..2632,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRV71454.1"
                     /translation="MRLTELSRSSGUAAKIGPADLEKILKDLPGQEEGFLMTSWAHGE
                     DAALWMVDEQRAGIFTLDFITPVVDDPFIFGQIAAANSLSDVFAMGGRPLVALNIVGF
                     PVNCQPLEVLTEILKGGQDRVAAAGAFLAGGHSVEDEEPKYGLAVYGEVLADRMWKIT
                     GARPGDSLILTKPLGTGLIITAFQAEMDEPGEIDAAVDSMRMLNDLPRRLPKDLSSAV
                     HACTDVTGFSLAGHLLDMLSGGGIGVRLFTGELPLLPGALEKADMGLVPAGSYRNREL
                     YSPRVDGLESVPSSLSDLIFDAQTSGGLLLAVPDEVSDEFVGLCKSSGFSHASKIGVF
                     TEGPGKISL"
     gene            3780..4289
                     /locus_tag="P5541_05420"
     CDS             3780..4289
                     /locus_tag="P5541_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRV71455.1"
                     /translation="MSKAERVLDFAMRMELEARDFYLDSLQKVSRADTRSLLSTLAEW
                     EKGHYDYLKGHKESLAGSGEWASGAPFPDEIAKGERIVREKEKGAETDPPLGEASSDF
                     SVLRMALAIETDFAKFYGNAAKNMEDPKGKEILQMLSEWEGGHQDYINEQYRALHREF
                     MDEMGFEPF"
     gene            complement(4258..4920)
                     /locus_tag="P5541_05425"
     CDS             complement(4258..4920)
                     /locus_tag="P5541_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71456.1"
                     /translation="MNHTEINVFRLTEQETKRLKAAVPAVGATLLLLFLVITCGETSV
                     TEAAFPLLFLPLVSLTSFVISMNRAEDLLGDMWFRRSLQIAGAILAATLAESLPFFLF
                     FRDWGLNWMPFIVGVILASAISGLFGAVSGALFPGKGSIASWFAAAFLQTPSLTGFAL
                     IRHPGLFLLPNQAALDLLDGAFGPLNGIDLMLPLITGTLWITAGIFVTVLLRRARTPS
                     RP"
     gene            complement(4917..5495)
                     /locus_tag="P5541_05430"
     CDS             complement(4917..5495)
                     /locus_tag="P5541_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRV71457.1"
                     /translation="MDKTKIVSRLLEVIEKDILPITREGVNEGHNIFGGAVLAWDDLS
                     LLTAGTNKRGEDPTLHGEMVTIRHFFRMKDRPSPEETVFLSTHEPCSMCLSALAWSGF
                     KKIFFLFGYEETRDDFRMGDDLQILAEVFSTTTPSRKNRFFELTPIRSMLRGIPEREA
                     LASRIETIREEYILLAERVFETEGNRPGGAAE"
     gene            5620..6630
                     /locus_tag="P5541_05435"
     CDS             5620..6630
                     /locus_tag="P5541_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71458.1"
                     /translation="MSGSKAVFSAGTDNLASSLFESRSTGVCLIQEGLVKTINPVLED
                     LTGFRSEDIQGKPWGDLVHPEDRNLFLSGSEKNLGAMENPIHFRMKSKEGGTLWMTGT
                     LTPTVFHARPALLALLADVSEHEKIHEEFRSREEDLLKTTEGTLRAIEKIVEMKDPFI
                     AGHQRRVARLSYAIARHLDFPESSAKTLRMAALVHDVGKVSIPIQVLAKPMHLTDDEY
                     SIVRMHPLIGYEILKDIDHLAFVGEIILQHHERIDGSGYPSGLTGDQMLPESRIISVA
                     DVVEAMVSQRPHRPAMGIEEALAEIRKGRGVEYDMEVADACVALFHDLGFTLEAESEG
                     AF"
     gene            complement(6641..7144)
                     /locus_tag="P5541_05440"
     CDS             complement(6641..7144)
                     /locus_tag="P5541_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRV71459.1"
                     /translation="MGLEEQVFSLSKVALAMILGGIIGLEREMIGKPTGMRTHMLIGG
                     ASALFTLLGSVIIVYFGEVRCGTDLISSDPIRVIQAIALGISFIGAGTIMQHPERRKV
                     IYLTSAASILFTAGIGIAVAIDQFLLAGGCVVIVWIVNRGLRYLDKKVLKKRNPVFRM
                     DETDRHE"
     gene            7184..8530
                     /locus_tag="P5541_05445"
     CDS             7184..8530
                     /locus_tag="P5541_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRV71460.1"
                     /translation="MISQRCPLSRANLFFLFIPVIVLAFLFSPFSSRACAVTQNALRF
                     VDESFDKPLVPVSRIMVLQQEFKERFFSPWNREIPSISPENALWAFDAWKERLPWGEN
                     LRPRSREFLESLKVNCAMKDWGSLNRKTVACRDTDLRALPTDRPFFRDPSLAGEGFPF
                     DYLQNSRVKAGEPLFAGHFSADGRWLFVETGYAAGWVDSRDAALVDEGLAQIWRESPL
                     AFVIKDDVPVKDEKGSYMFSAKIGTILPIFSAGVRGGPIRAGVPARDRDGNAFLEDTL
                     LPAGSTSNGPLEFTRWNVALMVNEMLGTPYGWGDSLGNRDCSGAMRDLFAVFGIWLPR
                     NSAAQAKAYHFVSLEGLTGEEKEKTLLLKGKPFATLLRIPGHIMLYLGEYRGKPCVFH
                     SFWGVRTSRGGIEGRHVFGEAVITTLRPGREVDDFDTSRGDLLDRLAGMTLLTVGP"
     gene            complement(8527..10236)
                     /locus_tag="P5541_05450"
     CDS             complement(8527..10236)
                     /locus_tag="P5541_05450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71461.1"
                     /translation="MNISLFVLALSLIFQTAGTLAAMRLLIVARKHPAVVLVAVTGVL
                     MLFRRLRMLLFVMSDNALPEFFLLQESLGLTVSLFLLASVLWAVPRLLGLLQTRDELA
                     EKHSTASQIIKDLAIPAFVINTDHKVTHWNKACERLTGVSAMDMIGSDEPWRAFYGEK
                     RPVLADLVLEGAGLDRVKKIYGDSCSPLEGTRGGLEAEFFLPKINGGRWLSFMAVPLF
                     GPGGTISGAIETFLDRTTEREKGLSLSRSASRLRALHEILQELAGENEIAPLMEKTVR
                     LLDREMSIINVSIVKNTSTDKEKTFTPLIIASTSIDPSEYGITSDRLRMSGKGLSVRS
                     AREKRMIVTPDVSSSPDFFQFVESSVSEIDVPIMDGERVLGVISAEGTGPFDTSDEEL
                     FTILAGHLSSLWKNIELVKEMESMALTDSVTGTPNRRALLQRLEEEENRLSRSGGKIS
                     LVMLDMNRFKEVNDTYGHFMGDAALKSAAETISDSLRSCDFAARFGGDEFVILLPDTG
                     PAEADVVIDRIIRGLGNLRVKGIPMNLSAEYGLACCPDDGRKLSSVLKSADDRMNANK
                     NRG"
     gene            10382..11320
                     /locus_tag="P5541_05455"
     CDS             10382..11320
                     /locus_tag="P5541_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71462.1"
                     /translation="MSGKPVLDSPEREKIFRRDRFTCQFCGRTFPDVLLDVDASPLFR
                     KQGKEMVTSCYECTRRNWLNRESTSRPEGTGADDAVVLEWKRQKDWYSRWLDEQNMPG
                     PKVGQEADQDSDTEQEAVVDNITRADENLKQVFAPEANLDDEIIEIEPEQEQEPEQEQ
                     EPEPEPEPEPEPEPELRLHNENDGAPDLPSGGSPQGITQKDISSGSGRVPPDLSPFWF
                     RSWLLYTLMKVSGLDPMDAEEAFIMTVTGMENAGALPEGEGLLFIDSLRDAAQGLAGQ
                     GYLVADGTKGTIRLTETGITVSRMIVPPGLSSRDGE"
     gene            complement(11365..14160)
                     /locus_tag="P5541_05460"
     CDS             complement(11365..14160)
                     /locus_tag="P5541_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRV71463.1"
                     /translation="MSMRDIFDGSPFGGGGGQKRQAPPLKLKKGVWILFGIVILLAVV
                     IPATATFYTDMLWFRAKGFSQVFWTRLIPQWILFALAAATAFLFFSLNWLRARKSAIT
                     GMTEYTDGTEVVPLRASALLVLAVSAVMAVMNGLGVRSEWMTVLQFLNRTPFGKGEPL
                     FGKDVAFYVFDLPFWSLIQGWVMGTLVTATLGVALIFFMASLPRMREENRLYIPASAR
                     THLSILGAVAVIAWGAGIWLERYGLLLSEGGIVFGAGYTDIHARLFAINIMALLSLVV
                     AALLVANTFRRTWRLAIAGIVLMVGASVILRGVVPGIIQKYVVEPNEFSKERPYIEFN
                     INNTLAAYGLDSLSVIDFTPDDSITRADIENETDTISNIRLWDYRPLLRAFKQLQEIR
                     TYYDFPDVDIARYTFDGTYRQVMLAARELDVDQIQNPTWVNRHLEFTHGFGIVMNFVN
                     EVDRAGKPVLIIQDVPPKISVPLDIDQPRIYFGEKRLPYSLVKTDVLEFDYPLGNSNM
                     RTTYEGTGGVAIGGLFNRIMFSLRFRDSQILFTNVIKPESRILMFRSIRERLNVVAPF
                     LLYDSDPYLAVMGGRLVWIQDAYTATSRYPYSNPVTISGTRQKVNYVRNSVKAVIDAY
                     DGSMFFYVADTDDPLLKTWQGIFPDLFTPIEDAHPEFCTQIRYPMGLFLIQSEIYRTY
                     HMTDPNTFYNKEDVWRLSTQTSSGNLNAYYVIMRLAGEEKAEFALIAPFMPVGRDNMI
                     AWMAGRSDVPNYGELLVYKFPKQKLIFGPSQVAALIDQDPEISAQLSLWSQRGSDVIR
                     GDILVIPLGNSLLYAQPLYLRAENSDLPELKRVIVSSGGKVAWAETVDDALSILISRL
                     SGSTGARVSTPTPTPDRPDERPPVMLGDNARDLSAQARQHFEAAQRASREGNWARYGE
                     EIEKLGEVLEALMRETE"
     gene            complement(14288..15226)
                     /locus_tag="P5541_05465"
     CDS             complement(14288..15226)
                     /locus_tag="P5541_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV71464.1"
                     /translation="MKGKTLVFMLLFAVAVVGFLVYNNTRQPAWEEVAPGDDPLAYEG
                     TVKVGAQTVNESIVLAWMGKLLVENHTGLSVDINTEFAASSVLHQAMVGRELDIYPSW
                     TGTQLTGILRYEGPNLPSEETFKMVKEGFEKTFQMTWAAPIGFNNTYVMAIPGAKAEE
                     LGLKKASDLKGHSENWKLASDENFDTRPDGYPGWTEAYGIEFGEVLPMQYSIMYRAIA
                     EGEVEAIVAYSTDSRIKKLGLALLEDDKSFFPDYSAAFVVRMDTLERYPALLSILEKL
                     SGQIDEVTMSELNGRFDDGEEPEDIAKDFLTQAGLI"
     gene            complement(15268..15966)
                     /locus_tag="P5541_05470"
     CDS             complement(15268..15966)
                     /locus_tag="P5541_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71465.1"
                     /translation="MPDQKPLLDLFLAYIIRFWPRILSLTWQHIMISSIALAITLAVC
                     IPLGIYLTRNEKAAPYVIGVANIFQTIPSLALLGFLIFIFGIGNDNAICALFLYAMLP
                     VLQNTYTGLKNVPKHLILAARGMGMTEYQILMRVQMPLGLPVIIAGIRVATVWIIGTA
                     TLASAIGGGGLGRLIFSGLASIRNEVVLAGALPATILALVADQALKMFQAHADPKRRA
                     LRLSQKTETSADRP"
     gene            complement(15966..17099)
                     /locus_tag="P5541_05475"
     CDS             complement(15966..17099)
                     /locus_tag="P5541_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HRV71466.1"
                     /translation="MVIFDKATKIYEDGTRAVDRLDLEIERGEIAVLIGPSGCGKTTS
                     LKMVNRLEELTSGKIVVGGKDIHSLDPVELRRSIGYVVQEIALMPHMSVAENIAIVPR
                     LLGWDKKRIRRRVDELLELARLDPARYRFRLPDQLSGGQKQRIGVLRALAADPEVVLM
                     DEPFGALDPISREGLQNELLELQNTVKKTIIFVTHDMEEALRMANRVVIMRQGRIEQM
                     GTPDEIQDNPATDFVRNFIGEDRLANISPDSPLDILVQEPILRVKPGRAASEVLDMME
                     DEGCETAQVVDKNGKWHGMALLWLLKQASRKGLSAADGLTRERKLLIEEATLRDAAEM
                     LADKDLPIPVVNETGEFVGIVTQAGVARLTIGRLTRKRREVKD"
     gene            complement(17083..18246)
                     /gene="hisC"
                     /locus_tag="P5541_05480"
     CDS             complement(17083..18246)
                     /gene="hisC"
                     /locus_tag="P5541_05480"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV71467.1"
                     /translation="MSVEGIRKAVLGMEPYVPGKTIEEVRRELGLDRIVKLGSNENPY
                     APFPASAAAMRDEIENINRYPDIAFEEIKELLAGIHGVDPKNIAISHGAEGMLQAMAK
                     CFLEPGDEVLLPSATYSLYRELSKLMGGRIVDVPLKDHTLDLEAVSDAATERAKLVWL
                     CNPNNPTGTVFHPGLLDGLLDSLPSTAWVVLDEAYAEFAEQDLLPDRARLVSGGRNVI
                     SIRTFSKAYGLAGARIGYAIAREEVITAIDTVSEPFNANRVGIAGAVAVLTLDREACE
                     RARQMIISDRGKTSDALKEMGLEVAPSHTNFIFFETPFPADAVAEDLLKQGIIVRPCG
                     GWGYDRAIRVTIGTGEENSAFLAALGRTIEQMKYRTGESPGKPAKEVAHGYLR"
     gene            complement(18236..19873)
                     /locus_tag="P5541_05485"
     CDS             complement(18236..19873)
                     /locus_tag="P5541_05485"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HRV71468.1"
                     /translation="MIGPNGARRVVKTLESLGVSVIFGIPGIHNLDIYTALTESDICH
                     VTARHEQGAGFMADGYARSTGETGVALVISGPGLTNILTPMAQALHDSIPMLVISSQI
                     PAPYIGRGCGFLHELPDSVALAGGVSKQSFRVNLPGEIQEIIESAWHLASSGRPGPVH
                     VEIPMDILAGKCPGDQCPSAGTSNPAMPPLPEKALNQAADLLGNASFPVIIVGGGARK
                     AREEITSLAEKSGAIVIESCAGKGIVDERNPLCLGARIHFPCVREYLGSSDLILAFGT
                     ELSPTDLWEKPLPKKGPLVQIDLDPSNFQRNGDADIGIIADAGQAASALLEMVSTDPD
                     RRERASRAASILRSRSLDELRSVTAMGTDLEAMSGICASLRGAIPEEGILFADMTGPA
                     YVALSEYPAFRPGTFLHPVGFGTLGLAMPAAIGARLARPSRPVCVLAGDGGFQFTLPE
                     LAVAVQERLPIPIVIWNDHGFGEIRRNENARHPGVFIAVDNTTPDLELLASAYGAEYS
                     RPRDAKDLASSIVQAFSADVPTLIECSPGKEGFANER"
BASE COUNT         4433 a         5431 c         5756 g         4542 t
ORIGIN      
        1 gacgggagac aggagacggg agacgggaga caggagacag gggacgggag acaggagaca
       61 ggaagggcaa gccccgtgag acgtgagacg tgagacgaaa atcaagggcg tgaacggtga
      121 acgggggccg atcgggtccc ccgatacatc cctgttccat tttgttccct ttttgatctc
      181 tttctgatat ccttctcgcg gaaaagcaaa aaacccgaaa tgaagggagg ttccgccatg
      241 gccaaccgga gtcgatgagc agtcacacga gtgcgggttc cgcgctcatt cgtcgttagt
      301 gatgactgct gcccaacttc tgtcccggaa ggtcgccgga actgtccctg attcttctcc
      361 gatggtattt tccatccttt cctctccggc tgaaaaaaga tttttctaaa acctcgggca
      421 gcgtggtcgc cgatctgcat gcgcgtacgt tcatgtgtgt tgctcttcgc ctgccggaag
      481 cttgatcctg acgagatcaa ccggagtaat aaaacatcac aaaatggaaa ggtggagttg
      541 aaggtaatgt tttcaagcct taacagacta ttgatgtccc ggatgggacc ggcggtcacg
      601 ggagcggttg tagggatcct cgcgcccctg ctcagtttct ggggcaaccc tgccaacatg
      661 ggaatatgcg tcgcctgctt cagcagggac attgccgggg ccctgggaat gcacagggct
      721 gcggtggtgc agtacatcag gccggaactt tccggcttca tcctcggatc tctagcggcg
      781 gctcttgttt acagggagtt caaacccagg gcaggttcct ccccgataat acggttcttc
      841 cttggcatgt tcgccatgat cggggcgctg gtctttctgg gttgcccctg gagagcctat
      901 atccgcctca gcgccgggga tctgaccgcc atcgcgggta ttgtcgggct caccgttggc
      961 gtggtcatag gcatctattt cctctggacc ggtttcagcc ttggacgaag ttaccaggct
     1021 aaaaaaccga tggggatgat catgccggct ctggcagtct cccttatggc tctcctgata
     1081 ttggctccgt cctttggccc tgacggaacc gggccgatat tcttctcgga aaagggacct
     1141 ggttcgcagt ttgccccgat ctggatagcc ctcggcgttg gtcttattgt gggctggctg
     1201 gcccagcgca gccgtttctg caccgtcggc gccattcgtg acctgatcat gctgaaggat
     1261 ggtcacctct tcaagggtgt cctggccttc atcgttgctg cattcgttac aaacttcctt
     1321 ctggggcagt tcaaccccgg catggaggga cagcccgtag ctcacaccaa tcatctctgg
     1381 aacttcatgg ggatggttct ttcgggtctg gccttcaccc ttgcgggcgg atgtccgggc
     1441 agacagttca taatgagcgg cgaaggtgac ggcgactccg ggatatttat cctcggcatg
     1501 atcgttggcg ccggtatcgc gcacaccttc agttttgcca gttccccggc tggacccgga
     1561 cctttcggtc ccggtgctac catcgtgggg ctgatcttct gtcttgccat cggtttcctc
     1621 atgagagaaa aagtaagcgt ataggaggga aaaaatatga gcgaaagtac aacagttgac
     1681 gcccggggtc tatcctgtcc ccagcctgtg gtggagacca aaaaggcaat gaacgcccag
     1741 cccgggggta aaatagaggt gttggtcgat acggtaacct cccgggaaaa tgtttcgagg
     1801 ctggcacgta accagggatg ggaggtttcg gcggaggatc tccccgacgg cggattcagg
     1861 gttattctta caaaggcgta aacggtaaac ggtaaacggt gaacgggaag ggcgagagcc
     1921 ctgtcattct gagcggagcg aagcggaacg tccgcctacc ggcgggaatc cgggttggta
     1981 tttaaggttt tcctgcctcc tgtatccagt ctcctgcttc ctcgcttttt ccgattcacg
     2041 attcacgact cagggaagta aaaacagatc cttcgatcgc tacgctcctc aggatgacag
     2101 caacagattc aaaggcgtaa acaatcaacg gtgaacggga agggcgagag ccctgtcatt
     2161 ctgagcggag cgaagcggaa cgtccgccta ccggcgggaa tccgggttgg tatttaaggt
     2221 tttcctgcct cctgtatcca gtctcctgct tcctcgcttt ttccgattca cgattcacga
     2281 ctcagggaag taaaaacaga tccttcgatc gctacgctnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnaa aacagatcct tcgatcgcta cgctcctcag gatgacagca
     2461 acagattcaa aggtgtaaac ggtaaacggt gaacggtgtg gatcaaaaaa tcggaaaagg
     2521 caggaccgga actggtccct actgtttacc actcactgtt taccgttcac cggtttttta
     2581 gaaaggaggc tcccccatgc ggttgacgga actttcgcgc agcagcggct gagcggccaa
     2641 gataggtccg gcggaccttg aaaaaatact taaagatctc cccggccagg aggaagggtt
     2701 ccttatgacc agctgggctc acggtgagga tgcggccctc tggatggtgg acgaacagag
     2761 agcggggatt ttcacccttg atttcataac tcctgttgtg gatgatccct tcatcttcgg
     2821 ccagatcgcc gccgccaact cgctgagcga tgtcttcgcc atgggggggc ggcccctggt
     2881 tgccctgaac atcgtggggt tccctgtcaa ttgccaaccc ctggaagtat tgacggagat
     2941 tctcaagggc gggcaggaca gggtcgcagc tgccggggca tttctggcgg gggggcacag
     3001 cgtcgaggac gaggagccca agtacggcct ggctgtctac ggcgaggttc ttgctgacag
     3061 gatgtggaag ataacaggcg cccggccggg agactccctg atccttacga agcccctcgg
     3121 caccggcctg ataataacgg cttttcaggc ggagatggac gaaccggggg agatcgacgc
     3181 ggcggtcgat tcaatgcgaa tgctcaacga ccttccgcga aggctaccga aagacctttc
     3241 cagcgcggtt catgcctgta cagacgtgac cggtttcagc ctggcggggc atcttcttga
     3301 catgctttcg ggaggcggca tcggggtcag gttgtttaca ggggaacttc cgcttttgcc
     3361 gggagctctc gaaaaagcgg atatgggact ggttccggca ggttcgtatc gtaacaggga
     3421 actctattcc cccagggtcg atggactaga aagtgttcct tcttcccttt cggacctgat
     3481 tttcgacgcc cagacatcgg ggggtctact gttggcggtt cccgacgaag tttcagacga
     3541 atttgtcggt ctttgcaaat catccgggtt ctcccatgcc tcgaagatag gggtttttac
     3601 cgaaggcccg ggcaaaatat ctctttaaaa caaatccagc aaacatgagg cggctcaccc
     3661 cgtgtttcgg gggggccgct ttttgtattc attatcccag ccatcaggcc ggtctgcccc
     3721 tgtttcattc tggtaaaata gtgatgcttt cgataaccat ttcctggagg tgtcaaaata
     3781 tgagcaaggc ggagcgtgtt ctggatttcg ccatgagaat ggaacttgaa gccagggatt
     3841 tctatctcga ctcccttcag aaggtcagcc gggccgacac aaggagcctt ctctctacct
     3901 tggcggagtg ggagaagggc cattacgact atctcaaggg gcataaagag agtcttgccg
     3961 ggtcggggga atgggcctcc ggcgcgccgt ttcccgatga gatagcaaaa ggcgaaagga
     4021 tcgtcaggga gaaagaaaaa ggtgccgaga ccgatcctcc ccttggtgag gcatcatcgg
     4081 atttcagtgt tctgaggatg gccctggcca tagagaccga tttcgcaaag ttttatggaa
     4141 acgccgcaaa aaacatggag gatccgaaag ggaaagaaat tctccagatg ctctccgaat
     4201 gggagggtgg tcaccaggac tatatcaacg agcaataccg ggcattgcac agggagttca
     4261 tggacgagat ggggttcgag cccttctgag caggaccgta acgaaaatac ccgcagttat
     4321 ccagagggtc cccgttatca gcggcagcat gaggtctatc ccgtttaggg ggccgaaggc
     4381 gccgtccagc aggtccagtg cggcctggtt gggtagaagg aagagtcccg ggtgacggat
     4441 aagcgcgaag cccgtcaggg aaggggtctg aaggaaggca gccgcgaacc atgaagcaat
     4501 gcttcccttc cctgggaaaa gtgcgcccga caccgctccg aagagaccgc tgatcgcgga
     4561 ggcaaggatc acacctacga taaagggcat ccagttgaga ccccagtctc tgaaaaagag
     4621 aaaaaagggg aggctctcgg cgagtgttgc cgccaggatg gcccccgcga tttgcaagga
     4681 acggcgaaac cacatatctc ccagcagatc ctccgccctg ttcattgaga taacaaaaga
     4741 tgtcagcgac acaagaggga gaaaaaggag cgggaatgcg gcctcggtga cgcttgtttc
     4801 cccgcaggtg atcacgagga agagaagcag caatgtagcc ccgactgccg ggaccgccgc
     4861 cttcagtctt ttcgtttcct gttcagtgag tctgaagacg ttgatctcgg tgtggttcat
     4921 tcagcagctc ccccgggtct gttcccttct gtttcgaata ccctttcggc aagcaagatg
     4981 tactcctctc ttatcgtctc tatccttgat gcaagtgcct ccctttcggg tatcccgcgc
     5041 aacatgctcc ttatgggagt cagttcgaaa aaccggtttt tccgcgatgg agtggtcgtg
     5101 gaaaagacct cggccagtat ttgcaggtcg tctcccatcc tgaagtcgtc ccttgtctcc
     5161 tcgtagccga aaaggaagaa aatctttttg aacccggacc aggcaagggc ggaaagacac
     5221 atggaacagg gttcgtgggt cgaaagaaag acggtttctt ccggagaggg tcggtccttc
     5281 atccggaaga aatgtctgat tgtgaccatt tcaccgtgaa gagtagggtc ttcccccctt
     5341 ttgttggtcc ccgcggtcag tagggaaagg tcatcccatg caagaactgc tccaccaaaa
     5401 atattgtgcc cctcatttac gccttccctt gttatgggca ggatgtcctt ctcgattacc
     5461 tccagaagcc ttgaaacgat cttggtttta tccatggaaa tccctccgat acccacaaag
     5521 atagcagaat aaccccgtag tggaaccccc gaaatttgcg acttgatctg tgtgatagaa
     5581 taatggcact tgaggatcat ccacctggag gtgggttaaa tgagtggcag caaagcggtt
     5641 ttttccgctg gaacggataa tctggcttct tctctcttcg aaagcaggtc aacgggcgtt
     5701 tgccttattc aggaagggtt ggtaaagacc atcaaccctg tccttgaaga tctcaccggt
     5761 ttcaggtcgg aggatatcca gggaaagcca tggggcgacc tggtccatcc cgaggataga
     5821 aacctctttt tgtccggttc ggaaaagaac ctgggggcga tggaaaaccc catccacttc
     5881 cggatgaaaa gcaaagaagg gggtaccctg tggatgaccg ggaccctgac cccgacggtc
     5941 tttcacgctc gccctgccct ccttgccctt cttgctgatg tctccgaaca cgagaagatc
     6001 cacgaggagt tcaggagcag agaggaagac cttctaaaga cgaccgaagg gaccctcagg
     6061 gccatagaaa agatcgtgga aatgaaggac cctttcatcg ccggacacca gagaagggtt
     6121 gccaggctct cctatgccat tgcccgtcac ctcgactttc cggaatcttc ggccaaaacc
     6181 ctcaggatgg ctgccctggt tcacgacgtg gggaaggtat ccataccgat ccaggtcctt
     6241 gccaaaccga tgcacctgac agacgacgaa tatagcatcg tcaggatgca cccccttatt
     6301 ggatacgaga tcctcaagga catagaccac ctggcattcg tcggggagat catattgcag
     6361 catcacgagc gcattgacgg ctcgggatat ccttcaggcc ttacggggga ccagatgctg
     6421 cccgagtcaa ggataatttc cgtggctgat gtggtggaag cgatggtctc acagaggccc
     6481 caccggcctg ccatgggtat cgaggaagct ctggccgaga tccggaaggg acggggagtg
     6541 gaatacgaca tggaagtggc cgatgcctgc gttgcccttt ttcatgacct tggttttacc
     6601 ctggaagcgg agtcggaggg ggccttttag ggggccttgg tcactcgtgt cggtcggttt
     6661 cgtccatcct gaagacggga ttccttttct tgaggacctt cttgtccagg taccggagac
     6721 ccctgtttac gatccagaca attacaacgc accccccggc gagaagaaac tggtctatcg
     6781 ccacggcgat tcctatcccg gcggtgaaga ggatagatgc ggcagaggta aggtagatca
     6841 ccttgcgcct ctcggggtgt tgcattatcg tccccgctcc gatgaaactg atccccaggg
     6901 ctatggcctg tataacacgg atgggatcgc tgctgatgag gtctgtcccg catcggacct
     6961 ctccgaaata gacgattatc acactcccga gaagggtgaa gagagcggac gcacccccga
     7021 taagcatgtg agtcctcatt ccggtcggtt ttccgatcat ttccctctcg agcccgatta
     7081 ttccccccag gatcattgcc aaagcgacct tggagagaga gaatacctgt tcctccagcc
     7141 ccatgaagat acctccttcc ggataaggaa accttctgga tatatgatat cccaaagatg
     7201 ccccctgagt cgggcgaacc tgttttttct ttttatcccg gtcatcgttc ttgccttcct
     7261 cttttctcca ttttcatcac gggcctgcgc tgttacccag aatgccctcc gtttcgtcga
     7321 tgaaagtttc gacaagccgc tggtcccggt ttccaggatc atggtccttc agcaggagtt
     7381 caaagaaagg ttcttctctc cctggaacag ggagatccct tccatctcac ccgaaaatgc
     7441 cctatgggct ttcgatgcct ggaaggaaag gttgccctgg ggcgaaaacc tgaggccccg
     7501 ttcccgggaa tttctggaaa gcctcaaggt gaactgtgcc atgaaagatt ggggcagcct
     7561 gaaccgcaag actgtcgcct gcagggatac ggacctgcgc gccctcccga cagaccggcc
     7621 attcttccgt gacccgtccc tcgctggcga gggcttccct ttcgactatc ttcaaaattc
     7681 cagggtaaag gccggggaac ccctgtttgc ggggcatttt tcggccgacg gaagatggct
     7741 tttcgttgag accgggtacg cggccggatg ggtggattca agggacgcgg cccttgttga
     7801 cgaaggcctt gctcaaatat ggagggaaag ccccctcgca tttgtcataa aagatgacgt
     7861 gccggtgaaa gacgagaaag gttcctacat gttttccgcg aagatcggga cgatcctccc
     7921 gatattttct gccggagtca ggggagggcc aataagagca ggtgttcctg caagggaccg
     7981 ggacggcaat gcttttctgg aggatacgct tctgcccgcg gggtcaacgt cgaatggacc
     8041 cctggagttc acccggtgga acgttgcgct gatggtcaac gaaatgctgg ggactcccta
     8101 cgggtggggt gattctctgg ggaacaggga ttgctccggc gcgatgaggg acctcttcgc
     8161 tgtctttggt atatggctgc cgagaaactc cgcggcgcag gcaaaggcat accacttcgt
     8221 ttccctcgaa gggctcacag gggaagaaaa agagaaaacc ctcttgctga aaggaaaacc
     8281 tttcgcgact ctactgagga ttccggggca catcatgctc taccttggag agtacagggg
     8341 taagccctgt gtttttcatt ccttctgggg agttcgcacc tccagggggg gtatcgaagg
     8401 gcgtcacgtt tttggcgagg cggttataac aaccctgcgc cccgggagag aggtcgacga
     8461 ttttgatacc tcccgtgggg atcttctgga tcgtctggca gggatgactc tgttgactgt
     8521 tggaccctag cccctgttct tgttggcgtt catgcggtca tcggcggact tgaggaccga
     8581 tgacagtttc ctgccgtcat cgggacagca tgcgagtccg tattcggcgg agaggttcat
     8641 ggggataccc tttaccctca ggttcccaag gcccctgata atccggtcta ttaccacatc
     8701 tgcttctgca ggcccggtgt ccggaagcag gatgacgaac tcgtccccac cgaaacgggc
     8761 cgcaaaatca cagctgcgta gtgagtccga tattgtttct gccgctgact tgagagccgc
     8821 gtctcccatg aaatgtccgt atgtgtcgtt aacttccttg aaccggttca tgtccagcat
     8881 gaccagggag atttttcctc cgctccggga aaggcggttc tcctcttcct cgagcctctg
     8941 cagaagtgcc cgccggtttg gtgtcccagt gaccgaatcc gtcagggcca tggattccat
     9001 ctcttttacc agttcgatat ttttccagag cgatgacagg tgtcccgcaa gaatggtgaa
     9061 aagttcctcg tcggaggtgt caaaaggccc ggttccctct gccgagatga caccgagaac
     9121 cctctctccg tccatgatgg gaacatctat ttcggagacg ctactctcaa caaactggaa
     9181 aaagtcggga gaagaagaca catccggggt aactatcatg cgtttctctc tggcagatct
     9241 gacggagagc cctttacccg acatcctgag cctgtcgctt gtgatgccgt attcagaagg
     9301 gtcgatcgag gtggacgcga ttataagggg ggtgaaggtt ttttctttat ctgtcgatgt
     9361 atttttcaca atgcttacat ttataatgga catctcccgg tcgagaagcc tcacggtttt
     9421 ctccatcagg ggggcaattt cgttttcccc agccagttcc tgcaatattt cgtgaagcgc
     9481 ccgcagtctt gatgcgctcc ttgagaggga aagccctttt tcccgttccg ttgtcctgtc
     9541 gaggaaagtc tcgatggcac cgctgatggt gcccccaggt ccgaaaagag ggactgccat
     9601 gaaggagagc caccggccac cgttgatctt gggaaggaaa aactccgctt ccagtccgcc
     9661 cctggtgcct tcaaggggag agcaggagtc accgtatatt ttctttaccc tgtccagccc
     9721 ggcaccctcc agaacaaggt ccgccaggac cggtctcttt tccccgtaga aggccctcca
     9781 gggttcatcg gagccgatca tgtccatggc ggatactccg gtaagcctct cgcaggcttt
     9841 gttccagtgg gtgactttgt ggtctgtgtt tatcacgaat gccggtatgg caaggtcttt
     9901 aatgatctgc gatgctgtgg agtgtttttc cgcaagttcg tcccttgttt gcagaagccc
     9961 cagcaggcgt ggcaccgccc acaggaccga agcaagaaga aacagggaga cggtcaggcc
    10021 caaagattcc tggagcagga aaaactccgg gagggcattg tctgacatga caaaaaggag
    10081 cattcgcagc ctgcggaaaa gcataagaac tcccgtaacg gcaacaagaa ccaccgcggg
    10141 gtgtttcctt gcgacgataa ggagcctcat ggccgcaagt gtcccggctg tctgaaaaat
    10201 cagtgaaagg gcaagaacaa acagggatat attcatttgg tgttcctctt tcctgtttac
    10261 ttgcaactta agctatttta aatctttaga atagtgaacg tcaacttttc gggatgccct
    10321 tctcgaggtc tcactgtgat atattgttca ggtggaaaga acactcaggg gagtgtgacc
    10381 aatgtctgga aagcctgttc tggatagtcc agaaagggaa aagatcttca ggagggacag
    10441 attcacctgc cagttctgcg gcaggacctt ccccgatgtc ctgctcgatg tggacgcatc
    10501 gcctctcttc cgcaaacagg ggaaggagat ggtgacttca tgctacgagt gcacccgcag
    10561 gaactggctc aacagggaga gcacttcccg cccggagggt accggtgccg atgatgccgt
    10621 tgtgctggag tggaaaaggc aaaaagactg gtattcccgt tggctggacg aacaaaacat
    10681 gcctggcccg aaggttggac aggaagcgga ccaggacagc gatacggagc aggaagcagt
    10741 agtggataac attaccagag ctgacgaaaa ccttaagcag gtctttgcac ccgaagcaaa
    10801 cctcgatgat gaaattatcg agatagaacc ggaacaggaa caggaaccag aacaggaaca
    10861 ggaaccagaa ccagaaccgg aaccggaacc agaaccggaa ccggaacttc ggcttcataa
    10921 tgaaaatgat ggggcacccg atttgccctc ggggggaagc cctcaaggga taacgcaaaa
    10981 agatatttcc tcaggctcgg ggagggttcc gccggatctt tcccctttct ggtttcggag
    11041 ctggcttctt tataccctga tgaaagtttc cgggctcgat cccatggacg ccgaagaggc
    11101 cttcatcatg accgtcaccg ggatggaaaa tgccggtgcc ctgcccgagg gagagggtct
    11161 tctgtttatc gattctctca gggatgcggc tcaaggcctt gccgggcagg gataccttgt
    11221 cgctgacgga acaaaaggaa ccatcaggct taccgagacc ggtataacgg tctcacggat
    11281 gatagtacct ccaggcctgt cttccaggga tggagaataa aaaagccccg ccatcaggcg
    11341 gggctttttg ctgtgggtcg ttatctattc cgtttccctc atgagagcct caaggacttc
    11401 cccgagtttt tcgatttcct ctccgtacct tgcccagttt ccttctcttg aggccctctg
    11461 ggctgcttca aagtgttgcc tcgcctgtgc cgagagatct cttgcattat ccccaagcat
    11521 gacaggaggg cgttcatcag gcctgtccgg agtcggggtc ggagtgctta ccctcgcccc
    11581 ggtggaaccg gaaagtcttg aaataaggat ggaaagagcg tcatccacag tctcggccca
    11641 ggcgaccttg ccgcccgagg aaacaatgac acgcttcagt tccggcaggt cgctgttctc
    11701 ggccctcagg tagagaggct gagcgtaaag cagcgaattg cccaggggga tgacgaggat
    11761 atcgcccctg attacgtcgg acccccgctg gctccacaga gacagctggg cggatatctc
    11821 cgggtcctgg tctatcaggg ccgctacctg tgagggaccg aagataagtt tctgcttcgg
    11881 gaacttgtag acgagaagtt ccccgtagtt gggaacatcg gagcgtcccg ccatccatgc
    11941 gatcatgttg tcccgcccca ccggcataaa gggagcgata agggcgaact cggccttttc
    12001 ctctccggca agcctcatta tcacgtagta ggcattcagg ttgccggaac tggtctgtgt
    12061 tgagagccgc cagacgtcct ccttgttgta gaaggtgttt gggtccgtca tgtggtatgt
    12121 ccggtagatc tcgctctgga tcaggaaaag ccccatcgga taacgtatct gggtgcaaaa
    12181 ttccgggtga gcgtcctcga tgggcgtgaa gagatccggg aaaattccct gccaggtctt
    12241 gagcagaggg tcgtcagtat cggcaacata aaagaacatc gatccgtcgt aagcgtctat
    12301 gacggccttt acgctgttgc ggacgtagtt gaccttttgg cgggttccac tgattgttac
    12361 gggattggag taagggtaac ggctcgtggc ggtatatgcg tcctggatcc acaccagccg
    12421 gccacccatt acggcaagat agggatcgga gtcgtaaagg aggaaagggg cgacaacatt
    12481 cagtcgctct ctgatgcttc ggaacatcag aatgcggctc tccggtttta taacgttggt
    12541 gaaaaggatc tgggagtccc tgaaccggag ggagaacatg atcctgttga agaggcctcc
    12601 gatagctacc ccgcctgtac cttcgtaggt cgttctcatg ttcgaattcc ccaaggggta
    12661 gtcgaactcc agcacgtcgg tcttgacgag agagtagggg agtctcttct ctccgaagta
    12721 gatgcgtggc tggtcaatat ccagaggtac agaaattttt ggggggacgt cctggattat
    12781 caggaccggt ttacccgctc tgtcaacctc gttgacaaag ttcataacta tcccgaatcc
    12841 gtgagtgaac tcgaggtgcc ggttgaccca ggtcgggttc tggatctggt cgacgtcaag
    12901 ttcgcgggcc gcaagcatta cctgcctgta ggttccgtca aaggtatagc gggctatgtc
    12961 cacatcgggg aagtcgtagt aggtcctgat ctcctgcaac tgtttgaagg ctctcagcag
    13021 gggtcggtaa tcccagaggc ggatgttgct gatggtgtct gtttcgttct cgatgtccgc
    13081 gcgggtaatg gagtcatcgg gggtgaagtc gatgaccgaa agagaatcca gcccgtaggc
    13141 cgccagggtg ttgttgatgt tgaattcaat atagggacgt tccttggaga actcgttggg
    13201 ttcaacaaca tatttctgga tgatcccggg tactacacct ctgaggataa cgcttgcgcc
    13261 taccatcagg acgatgcccg caattgccag gcgccaggtc ctgcggaagg tgtttgccac
    13321 caggagggcc gccactacca aagaaaggag ggccatgatg ttgattgcaa aaagcctcgc
    13381 gtgaatatcg gtgtagcccg ctccaaatac gatgccaccc tccgaaagga ggaggccgta
    13441 cctctcaagc cagatccctg ctccccaggc gataacggct accgcgccca ggatcgaaag
    13501 gtgggtcctc gcgctggcag gaatgtagag cctgttctcc tctctcatcc ttggcaggga
    13561 ggccatgaag aatatcaggg ccaccccgag ggttgccgtt acgagggttc ccattaccca
    13621 gccctggatc agcgaccaga atggaaggtc aaagacataa aaggctacat ccttgccgaa
    13681 aaggggttca cctttcccga agggtgtccg gttaaggaac tgcagcaccg tcatccattc
    13741 agaacggacg ccgagaccgt tcatgacggc catgaccgcc gaaactgcaa gcaccagcag
    13801 tgcggaggcc cgtaaaggca cgacctcggt tccgtcagta tattccgtca ttccggttat
    13861 ggcgcttttc ctggcccgga gccagttcag ggagaagaac aggaatgctg tcgccgccgc
    13921 aagagcaaaa agaatccact ggggaataag tcttgtccag aagacctggg agaacccctt
    13981 cgcgcggaac cacagcatgt cggtatagaa agtggccgtg gcggggatga ctaccgcaag
    14041 caaaatgacg ataccgaaca ggatccagac gccttttttg agtttcaggg ggggtgcctg
    14101 acgtttctgc cccccgcccc ccccgaaagg ggagccatcg aaaatatctc tcatggacat
    14161 ggcctatcgc ctcacatttt tcaggatggg tcaaacattt attccagtgc acaatgtacc
    14221 atgtcgagag aaaatcagca aaaaggccgc ccgcatcagc ggacggcccc tgttctcgag
    14281 cttctggcta tatcagccct gcctgtgtaa ggaagtcctt tgcgatatcc tcgggttcct
    14341 ctccatcatc aaatcttccg ttgagttccg acatggtgac ctcgtcgatc tgtccgctca
    14401 gtttctccag aatcgaaagc agggcggggt atctctcgag ggtgtccatc cggacaacga
    14461 aagccgcact gtaatcgggg aagaatgact tgtcatcttc cagaagagcc aggcccagct
    14521 tctttatgcg ggaatcggtg gaataggcga cgatcgcctc aacttccccc tccgcgatgg
    14581 ccctgtacat gatcgagtac tgcattggca ggacctcgcc gaactctata ccgtaggcct
    14641 cggtccaacc cgggtaaccg tcgggacggg tgtcgaagtt ctcgtccgag gcgagcttcc
    14701 agttttccga atgtcccttg aggtctgagg ccttcttcag tccgagttcc tcggcctttg
    14761 ctccggggat ggccattacg tatgtgttgt taaagcctat cggggctgcc caggtcatct
    14821 ggaaggtctt ttcgaacccc tccttgacca tcttgaatgt ctcttccgat ggaaggttgg
    14881 gtccttcgta cctgagaatg cccgttaact gggtgcctgt ccatgaaggg tagatatcga
    14941 gctctctgcc taccattgcc tggtgcagga ccgatgatgc cgcgaactcc gtgttgatat
    15001 ctacggaaag gcctgtatga ttctcgacca gcagtttgcc catccatgca agaactatgg
    15061 attcgtttac ggtctgggct cccaccttca cggtgccttc ataggcaaga gggtcatcgc
    15121 cgggagctac ctcttcccac gcgggttgcc tcgtattgtt gtagaccagg aatccgacta
    15181 cggccactgc gaacagcagc atgaacacaa gtgtttttcc tttcatgttt cccatctcct
    15241 ttcagatttg ttttcgacag gatgcgacta cggcctgtct gccgatgttt ccgttttttg
    15301 tgaaagacgc aaggccctgc gtttggggtc agcgtgagcc tggaacattt tcaatgcctg
    15361 atccgctacc agggcaagaa tcgtagcggg aagagccccg gcaagaacaa cctcgttccg
    15421 gatagatgca aggccggaga atatcagtct tccaagaccc ccgcctccga tggccgaagc
    15481 gagagtggca gtcccgatga tccagactgt tgcaaccctt atcccggcaa ttatcacggg
    15541 caggcccagg ggcatctgaa cgcgcatcag gatctggtac tccgtcatgc ccatgccccg
    15601 tgctgccagg atcagatgct tcgggacatt tttcagtccc gtataggtgt tctgcaggac
    15661 cggcagcatt gcgtagagaa agagcgcgca tatcgcgttg tcgttgccta tcccgaagat
    15721 gaagatcagg aaacccagaa gggcaaggct cggaatggtc tggaaaatgt tggccacgcc
    15781 gatgacgtag ggtgccgcct tttcgttcct ggtaaggtag atcccgaggg gaatgcatac
    15841 agccagcgtt atggcaagag ctatcgaaga tatcatgata tgctgccacg tcagggaaag
    15901 gatcctcggc cagaaacgta tgatgtaggc caggaaaaga tcaagaaggg gcttctggtc
    15961 cggcatcagt ccttaacctc ccttcttttg cgggtcaggc ggcctatggt gagtctggcc
    16021 acccccgcct gggtgacgat accgacgaat tctcctgttt cgttgactac cggtatgggg
    16081 agatccttgt cggcaagcat ttccgcggcg tcgcgcaggg ttgcctcctc gatcagcaac
    16141 ttacgttccc gcgtgagacc gtcggccgct gagagtccct ttcgtgaagc ctgtttgaga
    16201 agccagagaa gggccattcc gtgccatttg ccgttcttgt cgacgacctg ggctgtttca
    16261 cacccctcgt cctccatcat atccagcact tccgaagcgg ctcgtccagg tttgacccgg
    16321 agtatcggct cctggaccag gatgtccagg ggcgaatcgg gagaaatgtt ggccagcctg
    16381 tcctcgccga tgaagttcct cacgaaatcc gtcgcggggt tatcctggat ctcatcgggt
    16441 gtgcccatct gctctatgcg tccctggcgc atgatgacta ccctgttggc catgcgaagg
    16501 gcctcttcca tgtcgtgggt aacgaagatg atcgtctttt ttacagtgtt ctggagttcc
    16561 aggagctcgt tctgcagccc ctcgcggctt attgggtcga gggcaccgaa cggttcgtcc
    16621 atcaggacca cctccgggtc cgcagccaga gccctcagta caccaatacg ctgtttctgt
    16681 ccgccgctca actggtcggg aaggcggaac ctgtacctgg cggggtcaag cctcgcaagt
    16741 tccaggagct catcgacccg cctgcgtatg cgttttttgt cccatccgag aagccggggg
    16801 actatggcaa tgttctcggc cacggacatg tggggcataa gggctatctc ctggaccacg
    16861 tatcctatgc ttctgcggag ttcgacaggg tccagggagt gaatgtcttt gcctccgacc
    16921 actatcttgc cggaggtaag ttcctccagg cggttcacca tcttcaggga tgttgtcttt
    16981 ccgcacccgc tcggccctat aagtaccgct atctcaccgc gctctatttc aagatcaaga
    17041 cggtcgacag ccctggttcc gtcctcgtag atcttggtcg ccttatcgaa gataaccatg
    17101 ggccacctcc tttgccggtt ttcccgggga ttccccggtt ctgtatttca tctgctcgat
    17161 ggttctgccc agggccgcaa ggaaggcaga gttctcctca cctgtcccga tggtcaccct
    17221 gatcgccctg tcgtaacccc agccgccgca gggtcgaaca attattccct gtttaaggag
    17281 atcttcggcc acggcgtcgg cggggaaagg tgtctcgaaa aagatgaagt tggtgtgcga
    17341 gggggcgact tcgagaccca tctctttaag ggcatcagag gtttttcccc tgtccgaaat
    17401 gatcatttgc ctggcccttt cgcaggcttc cctgtcaagg gtcaggacag ccacggctcc
    17461 ggcaataccg acccgattgg cgttgaaggg ctcgctgacg gtgtctattg cggtaatgac
    17521 ctcctcccgg gcgatggcgt aaccgatcct tgcccccgcc agcccgtaag cctttgagaa
    17581 agtccttatg gatatcacgt ttcttccgcc ggagaccagc cttgccctgt cgggaagcag
    17641 gtcctgctcc gcgaactctg cgtaggcctc gtcgaggact acccaggcgg tcgacggaag
    17701 ggaatccagg agtccgtcaa ggagacccgg gtggaaaacg gttcccgtag ggttgttggg
    17761 gttgcagagc cagacaagtt tcgccctctc tgttgcggca tcggagacag cctcgagatc
    17821 taacgtgtga tctttgagtg gaacgtcaac gatccgtccc cccatcagct tcgagagttc
    17881 gcggtagagg ctgtaggtgg ccgaaggcag gagcacctcg tcaccgggtt ccagaaaaca
    17941 cttggccatg gcctggagca ttccctccgc cccgtgggaa atggcgatat tcttgggatc
    18001 cactccgtgg atcccggcga gaagttcctt gatctcctca aaagctatgt cggggtaccg
    18061 gttgatgttt tctatctcgt cccgcatcgc cgctgccgaa gcggggaacg gtgcgtaggg
    18121 attttcgttg gatccaagtt tgacgatcct gtcgaggccc agttcacgcc gaacctcctc
    18181 gatggtcttt cccggaacat agggttccat tccgagaacc gccttgcgga ttccttcaac
    18241 gctcattggc gaacccttcc ttcccgggac tgcactcaat cagtgtcggg acatcagctg
    18301 agaatgcctg gactatgctg ctggcaaggt ctttcgcatc ccgtggtctg gaatattcgg
    18361 ccccgtatgc ggaagcaaga agttcaaggt ccggcgtagt gttgtccacg gctataaaaa
    18421 cacccgggtg ccgcgcgttc tcgtttcggc ggatctcccc gaaaccatga tcgttccaga
    18481 tcactattgg tattggaagg cgctcctgaa cagcaacggc gagttccggg agggtgaact
    18541 ggaatccccc gtcacccgcg agaacacaga cggggcgtga aggtcttgcc agtcgtgcgc
    18601 caatggccgc aggcatggca agcccaaggg ttccgaatcc tacggggtgc aggaaagtcc
    18661 cgggccgaaa cgccggatac tcgcttagcg ctacgtatgc ggggcctgtc atatcggcaa
    18721 agaggatccc ttcttcgggg atcgctcccc tcagggaggc gcatattccg gacatggcct
    18781 ccaggtctgt ccccatggcg gtcacagacc ggagttcatc cagagacctg cttcggagta
    18841 tactggcggc cctggatgca cgctctcttc tgtccgggtc cgttgatacc atttccagga
    18901 gagccgaagc cgcctgcccc gcgtctgcga tgatgccaat atcggcatcg ccgttgcgct
    18961 ggaagttgga agggtcaaga tcgatctgta ccagcggtcc tttctttgga agcggttttt
    19021 cccacaggtc cgtgggagaa agttccgttc cgaaggccag gatcaggtcg gaactgccca
    19081 gatactccct cacgcaggga aaatggatcc ttgcccccag gcagagaggg ttcctttcgt
    19141 cgacgatccc tttgcccgcg caggattcaa ttactatcgc accggacttt tcagcgaggc
    19201 tggtgatctc ttcccgggct tttcgtgcgc cgcctccgac aatgatcacc ggaaatgaag
    19261 cgttacccag caggtcggcc gcctgattca gagccttttc gggaagagga ggcattgccg
    19321 ggttggaagt tcccgcggag gggcactggt cccccggaca ctttccggca agaatatcca
    19381 tagggatctc cacgtgtacc ggacccgggc gtccactgga agccagatgc caggccgatt
    19441 cgattatttc ctgaatttct ccggggagat tcacgcggaa actctgtttt gagactccgc
    19501 ccgcgagggc gacgctgtcg ggaagttcgt gaaggaaacc gcagcccctg ccaatgtaag
    19561 gtgcagggat ctggctggag ataacgagca tggggatcga atcatgaagg gcctgtgcca
    19621 tgggggtgag gatgtttgtg aggcctgggc ctgagatgac aagtgctacc cccgtctcac
    19681 ctgtggatct ggcataccca tctgccataa aacccgcgcc ctgttcgtgg cgggctgtaa
    19741 cgtggcaaat atcgctctct gtgagggcgg tatagatatc caggttatgt attcccggga
    19801 tgccgaaaat tacggaaaca cccagggact caagtgtttt taccacgcgt cttgcgccgt
    19861 ttggaccaat caccctacca cctcgatctg ggaaattatg ggggcttcca aaggagacag
    19921 aaagcctgtc cgacaccctt tctgggagac ggacaacgaa aaaacgcctg aaaccggaga
    19981 aaaaccactg gagtagtgcc ggagaaaaac tcttttggat agaaaaacaa aaaactccgt
    20041 taaccggaag cgagttcagg cgtaaaagtt caacccggac atgttacccg aaaagtcaca
    20101 aatcgtcaaa ttgtatggat aaggggaaca aatccaggag ccgggaatcg tggaaaaccg
    20161 aggaaacagg aaacaggaaa caggaaacag gaaacaggag acgggaaaca ggaaacagga
    20221 aacaggaaac aggaaacagg aaacaggaaa caggaaacag ga
//
LOCUS       DAOTIQ010000032        20189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3211_length_20189_cov_6.202791, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000032 DAOTIQ010000000
VERSION     DAOTIQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_20189_cov_6.202791"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..1131)
                     /locus_tag="P5541_05490"
     CDS             complement(415..1131)
                     /locus_tag="P5541_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71469.1"
                     /translation="MADTILKVQDIHCGYDSVPVIHGLSLEVAQGELVAIVGANGAGK
                     TTTMRTISGLMNPFSGSIHFEGKDISRMKAHETIRLGISYVPEGRRLFSKLTIQENLE
                     LGAFTINDRNAIDKSLKQVFELFPKLYDRRSQVAETMSGGEQQMVAIARGLMSAPRLL
                     LLDELSLGLMPSLVEKVMETVVSINRSGVTILLVEQMVQEALEVADRGYVIQTGKIVQ
                     HGTAKELLDSKEVRKAYMGL"
     gene            complement(1131..1883)
                     /locus_tag="P5541_05495"
     CDS             complement(1131..1883)
                     /locus_tag="P5541_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71470.1"
                     /translation="MALLEIRNVTMRFGSLVACNNVSFDVEKGSVVGLIGPNGAGKTT
                     LFNCISGFYKPTSGNMFFRGEEVTSLPAYEIARRGAVRTFQVVHPLKEMTVLDNVLVG
                     AFLRGADAETANRVARECISLCHLEEVSGQLAGGLTIGFKKRLEMARALATGPELLML
                     DEAMAGLTSTEIKAAVEILRNLKKRGITLLVVEHIMEAIMSVADKIVVLDGGEKISEG
                     NPEDVVEDERVITAYLGAKFSQRLKRSKEVSQ"
     gene            complement(1870..2886)
                     /locus_tag="P5541_05500"
     CDS             complement(1870..2886)
                     /locus_tag="P5541_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV71471.1"
                     /translation="MKISGKMTGKDLFWLGLLAAFAVIPLTPWFNHSSFAQHVMIQVL
                     LYSSMAQAWNILGGYCGQTSFGHSVFFGIGAYGTGMAVVRFGLIPWPGILAGAMVAAL
                     VALAISYPCFKLKGHYFAIATFAIVEIFNRLFMVWYWVGGAIGLDYGFTEPGWKNFVW
                     YQSKVGYFYGVLLLFFIVFGIVRWIEKNRLGFYLRAVREGQETAESLGVNSTRVKLSA
                     MALSAFLAALCGAFFAQYNYRVDPPMVMSLDMSMKFVLITILGGLGTFYGPLLGAALL
                     IPLQEYSRAYLGGLGGGVDLIIFGLLIIIVVVYQPKGIMGILDRFTKSIRQHREGGKQ
                     NGPS"
     gene            complement(2891..3751)
                     /locus_tag="P5541_05505"
     CDS             complement(2891..3751)
                     /locus_tag="P5541_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV71472.1"
                     /translation="MATFLQVAFDGLLNGLLYALVAAGLSLIWGVMDVINFAHGEYLM
                     IAMYLSYWLGFIFKIDPLISSIAGGAFVFIVGALTYRLIISRTIGKSAMSALLATFGL
                     SMLLKNICLNRFSPNFRLLQDTFVGGKTIDLAGIIIPVPQLVIGLVALALLFVIYRLV
                     KKTRLGWAIQATAMDKEAAELMGINTERIYLLVFGLGGACVGIAGGLLPSYLAVHPEV
                     GALFSLIAFVCVAMGGFGSIPGALLASLLIGLVEAFAGFYIAPVFKYVAVFGLYLVVV
                     MVRPRGLFGW"
     gene            complement(3920..5191)
                     /locus_tag="P5541_05510"
     CDS             complement(3920..5191)
                     /locus_tag="P5541_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV71473.1"
                     /translation="MRQNAFGKVLLLALAALLVMGTFAGAAFAEEKVIKIGTLFPLTG
                     PCALAGQRCQAAVETAAEVINNAHPEMEVPLAAQKGILDGYKFVLVHADHQGKPDVAK
                     SEAERLFDQEKVWGIIGSYNSAATKPASFVAELKQKVFMCGCSSSGALTERDFKYFFR
                     MAPTDKTESLEFVEFIEWANKTKNTDMKTVGLIYENTEFGKHAAEEGRMAAEAGGFKV
                     VADVAFNPGATNLNSEVQTLKAANPDAVFGACLGGDYTLWVRTMKQMDWLPKVALNYC
                     TGYQDPIIAGQLGDDANYFMGGTGYAPEFAELMPAVAAVEEIYKTKTDPSVPFDSDSI
                     QEAVALLVLAQAIEKSGSLDPTVVAETLHANEWESPLSMGGKVAFAPGGQNVKAMSML
                     TQLQGGQYKRIYPVDLADTEPVIPMVPWKDR"
     gene            complement(5491..6417)
                     /locus_tag="P5541_05515"
     CDS             complement(5491..6417)
                     /locus_tag="P5541_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV71474.1"
                     /translation="MSRENVIPAGFGKEFYEEIVSQLHDGLYIVDRDRVIRFWNRAAE
                     LITGFSAGEVVGRTCSSNILTHVDTEGNNLCTGNCPLSDVISGGQNREDEVFLHHKAG
                     HRVPVSVRVSPLKNEKGEVVGAVELFTDLREKEAVRIRLAELEKLAMIDNLTQIPNRR
                     FMEKEILGRIAEKDRLAVPFGVLFMDIDHFKKFNDTYGHDTGDEVLKFVARTLADNSR
                     PFDVCGRWGGEEFVGVIRNADPDELVRVAERFRMLIENSYLANNGEKLCVTISIGATS
                     HIDGESSESLLKRADSLLYKSKESGRNRVTQG"
     gene            complement(6414..7067)
                     /locus_tag="P5541_05520"
     CDS             complement(6414..7067)
                     /locus_tag="P5541_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71475.1"
                     /translation="MKWKGRFSRPVLLFLPIFFLMTRSCASGGVPVVFTETFIDTPLP
                     IVAVSINMTSGEIVEDDIPGPFMGTRFEGTFEILAHLADGTAVATPLNMIMNPQYPDE
                     PLIFLEGEWSLQFADFTGDGRPETNLGQHAGSNGNLYRVIGFLPSGEAVEMPFMDMPR
                     GIFVSDHNYSSEEIRATGSGEICFEWYDNTRGITVLERWEWDPVKKCFTRVGKEEKI"
     gene            7418..8623
                     /gene="rocD"
                     /locus_tag="P5541_05525"
     CDS             7418..8623
                     /gene="rocD"
                     /locus_tag="P5541_05525"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRV71476.1"
                     /translation="MAVNSSGQLMEMEHEYGAHNYHPLEVVISKAEGIWVEDPEGRRY
                     MDMLSAYSAVNQGHRHPRIIKALKDQADILTLTSRAFFNDRWPLFAKKLASFTGKEMI
                     LPMNTGAEAVETAIKTMRKWGYLVKGVEKDKAEIITFMENFHGRTVTIISFSVDEVAK
                     DYYGPYTPGFKVVPYDDLGTVEKAINKNTVGILVEPIQGEAGVVVPSDGFLKGLEDLS
                     RKNNLLLAVDEVQTGFCRTGKNFAYQHEGIDPDIIIMGKALGGGVFPVSAIAANKDVL
                     GVFEPGSHGSTFGGNPLACAVAAEAIDVLVDEKLAERARELGEYFMDGLKAIKSDKIK
                     MVRGKGLLIGVVLNESAGKARIYTTALKDRGLLCKETHDWIIRFAPPLVIKKEEIDKA
                     LEVIREVLG"
     gene            8800..9579
                     /locus_tag="P5541_05530"
     CDS             8800..9579
                     /locus_tag="P5541_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV71477.1"
                     /translation="MEYNKVLISGSEDHVAYLTLNRPERMNSFDSELAAELYEALAEL
                     DSSDDVRVIVIKGSGKHFCAGIDISELAGKSAMEYRDWVELMERPLYHISKIKKPVIA
                     CVHGVAAANGCGLAASADLVIASEDARMGLTAINLGLNCVGPVLAVVRCIGRKKALEF
                     LLSGELFGAREAQAMGLVNRVVPAAALEEETHKWALSFANKSPLALQTAKTAFYAAED
                     MDYEKQFAFMNEVFARLCTSRDAAEGVAAFLEKRTPEWEGR"
     gene            9603..10079
                     /locus_tag="P5541_05535"
     CDS             9603..10079
                     /locus_tag="P5541_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRV71478.1"
                     /translation="MEDTRPGWDEYFISIANVVSSRSTCLRRKVGAVIVRERQIVSTG
                     YNGAPKGTPHCLETGCLREKHGVPSGERHEICRGSHAEINAIAQAAAMGSATAGSVIY
                     CTHEPCSFCTKALINAGIRRIVFVNHYPDPIASLIREEAGIEVACIEETPATEDQR"
     gene            10086..12710
                     /locus_tag="P5541_05540"
     CDS             10086..12710
                     /locus_tag="P5541_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV71479.1"
                     /translation="MVIEITGRKKTAPKFMLDDLVGLEPIKGELRKIEAMLWLNRHRR
                     SGDLGELTLQSFHFSFRGNPGTGKTTVARLIGDIFHRYGLLRVGDVVEVDRSKLIGPT
                     PGDTETRTRKALKSALDGVLFIDEAYSLLGSRESSDPGIRALEVILKGMEDYRDALIV
                     VFAGYPVEMDALFDHVTGLRSRVPYHLEFPDYSPRELVDIAIFMAESENLALSDEASA
                     AFLAKMEERSSLPGFSNAREVRNLIDQAKASLSARLQTRRKVSRWDMKVITSVDLQDE
                     GSESFALLEQAKREMYRSPSEPGVRCAYARACSNAGLWSDVVSVLEPVRDSIPSEDLS
                     LFGRALYIIGERKRSWDVFSCLKTDGSFFQGLSAMWSGELQEASRLLKESAAQNTGDP
                     DIQLALAAGCFFEGDFSGSARSFSRGLELCGSGLPSLLLRDLPYEELRWKESSQNLAK
                     ALHFAYGNPDRARLCFTRALIDTGEKSALVSASELVFECIDRTPDDPSAHRMMSLIKE
                     SEGRPGEAAASLEISLEIEPRRTEDWRRLALLYEKTGQNEKAEEIFSDILEEDPTGGA
                     GLRLAAAAETRGDLQGANELYRKAWAAGLAGEERSICAVKLGVYSAAAGRFAEGARFL
                     MEAGNLAKEPEGAFWMARALIEEEKWQDAETLLLGVPSGGNMGNARLYWLCRTYIARR
                     DLFSAKNIQWGNDSEAYTRLARGVTAALSGDPSHAALLEGLPARDMGCDALFMLCSAR
                     ASVKDWEEARHLGNMAQISEYGPFLWERQIKRPREEAAYLAAIAEAHLGNWAAAREAF
                     RRSASALRHPGPVFAFGVSMVATGNIDEARKISTQIQMTSPSLSRKLDELITQNSGIR
                     KMMADPVDPGILDLYAFI"
     gene            12762..13343
                     /locus_tag="P5541_05545"
     CDS             12762..13343
                     /locus_tag="P5541_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRV71480.1"
                     /translation="MAVITRDEAMTMLREHNSEVAHIRHALAVEAAMRHFAKLHGEDE
                     GKWALAGLVHDIDWETTSEDPSRHTHVGAGWLKDAGYPDDIVRAVLAHGWGICSDVEP
                     LSAMEKHLYAVDELTGFVTAVALVRPGKSLQDLTVKSVKKKWKDKAFARGVDRDVIEK
                     GTSILDTPLEDLIENVIEALKPVEREIGLGEGC"
     gene            complement(13340..13816)
                     /locus_tag="P5541_05550"
     CDS             complement(13340..13816)
                     /locus_tag="P5541_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71481.1"
                     /translation="MKDRSTLLAVVIILLLSAVSGTVFWKYTKVVDQNRELREEISVA
                     KSELDKVRAVMESSNMKHFPPGPDWERIKTSQRPDPKTWLTESILSRDDLIPWEGVLG
                     GTMRIYDPSSVWFLGPNWCLAWAEDGHIGGYMLLRFETGTEDKEPFWRLIDSEMPR"
     gene            13917..15479
                     /locus_tag="P5541_05555"
     CDS             13917..15479
                     /locus_tag="P5541_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRV71482.1"
                     /translation="MKKLFLKVPELTARSLVLAAAGSVLIAGSSSYVALRIGALPWPT
                     IFVAVLSMAILKPFGATLREINVAHTGMSAGGLVAGGLAFTLPGVWMADPGSSMGVWT
                     AVAAALVGASLGVVFTGLLRKHFIEELDLPFPMGVAAAQTLQAGDQGGKGALTLFASC
                     GFAGVIAYLRDAAGVLPPLIGRGPLFSVWLSPMAVGIGFIIGPLYMGTWAAGAILAHW
                     FMVPVGLASGWFTDGATAAAFRNSLGIGVIVGAGIAVLFRGAAKSFLRTLATSAGNLR
                     FAAAASALSAVVLTFAAGVPLVPSLLATAGVWITVVMAATITGQTGIDPMEIFGILVL
                     LAVRSLWDIGGQEAFLVTVVVAVATGLAGDAMQDFRAGAILGTDHRAQLVSEAVGGIS
                     GAVAAAFTIFLIREAFGAMGPGTDLAAPQAFAVKSMIEGLPDRGAFILGLLSGLFLTV
                     AGVPCMTVGIGMYLPVAISLAAGAGGLSRFLANRFFPGSGERFSLVASGFLGGEGIAG
                     VLYAIWKVATLG"
     gene            15493..16437
                     /locus_tag="P5541_05560"
     CDS             15493..16437
                     /locus_tag="P5541_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRV71483.1"
                     /translation="MWRIGHAQDHEKGTGCTVLLFPEGACASCDVRGGAPGTRETDLL
                     APECLVDRITAIVLSGGSARGLSSADGVMRYCEEKGWGFDTGFGIVPIVPAACLFDLP
                     CGESGAWPDIFMGREACLSARGLDESPMGNVGAGTGATVGKYVGLDKAMKSGFGWSKK
                     TSGSLEVSAVVAVNAFGAIRGTSGNFIAGAVDEGRIIDPCSLFEKGGIWSGWGKNTTI
                     GAIVTNASLDKAQCRRVAIMGQTGLGATIFPVNSPFDGDTVFCAATSETHAEMILVGA
                     LAADAMAEAVRNAVTGTRSAFGLPATVDLEETNSVNNR"
     gene            16530..17165
                     /locus_tag="P5541_05565"
     CDS             16530..17165
                     /locus_tag="P5541_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV71484.1"
                     /translation="MTIRVILADDHPLTRKGLKDYIEKESDLDLVGEASDGTGAWELI
                     GKELPDVALLDIRMPGENGVSLARRIRDSGLKVIPVMLTSFDSQQYVIASLRAGARGY
                     ILKTVTPDELSRAIRIVAKGGLYLDGEVARSMEESDSAFESLSAREREVLLYAARGLS
                     SKEIARDLIISERTVQTHLGSIYDKLGARNKTEAMLLALKYGVVALEELIE"
     gene            17162..18532
                     /locus_tag="P5541_05570"
     CDS             17162..18532
                     /locus_tag="P5541_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRV71485.1"
                     /translation="MRRHLLIFLTLVIMIPSFSALLIGGIGLVQHERTMERVARSYVQ
                     DMAESVASRIEMGWNRTRTSPLSDRERFLRLRQITWGLSIPGWMAVVDVHGNILLSSS
                     GAEVLPLIWDRGIPVGSAMEVDAKDGDRYTLAAYPAGETGWYVVIAVSWDKLLGPMLR
                     FSRWPVMVGLIGLLGLVSVFALWKWLVAPLRILGNEVSSLQWGRDMPVADHPGAVFEI
                     RRLRQVLHQLASSAVDRAELVKRYVGDLVRVQEEERSRIAREIHDGPLQDVTALIHQI
                     RLALMESLPDGRDREHLDNAEEGARHAVREMRSLCDELSPPWLDLGLGQALEELGERL
                     SRYLQVEIVVEVDCCEDLPPEVTLAFFRVVQEAVHNSARHGKASRVAVSLGKAEDGVI
                     LDIEDNGTGFDANVNYEELRVRGHRGLANMAERMTLIGGTLLVEKGREGGTRVRCMLP
                     VTSVSV"
     gene            complement(18513..19028)
                     /locus_tag="P5541_05575"
     CDS             complement(18513..19028)
                     /locus_tag="P5541_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRV71486.1"
                     /translation="MDPFGALLIFAARLLDVSLGTFRILLLVRGKKMQAGITAFFESA
                     VYIIALGYVLQEGITGPLQLLAYAGGFAVGSYLGASLEERILSSFVLVEVVAPVTEEI
                     FGIVDLLREEGYGTTVVSGSGKTGERLILKVICQRNDINHVARIVGTKGFLFTSDVRG
                     VWGGYFRQKRK"
     gene            19159..>20189
                     /locus_tag="P5541_05580"
     CDS             19159..>20189
                     /locus_tag="P5541_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV71487.1"
                     /translation="MRLRVLGAAGEVTGSSYILEAGPSRIIVDFGLHQGGNEGKNSEP
                     LEFDPKSVDAVLLTHAHLDHTGRVPLLVKKGFSGKVIATAPTVELTDVIWADSAKIQK
                     EDAEWKSRKNSRKGLPPEEPLYDDDDVEKAVKMLVPVTYDEIIEPAPGFKVRFRDAGH
                     ILGSSNLEIWMNDNGEEIKIVFSGDLGPQKTVMERNPSVIEDADYVVIESTYGNREHR
                     TNEESRQEFREAMKEMLSDKGKVLIPTFVVDRAQRVLYELMLMQIEGILPSNVPIYFD
                     SPMGAKATAVYTKYISLLSSEIQSQVRSGNDPFSPENLNIVSSVEESKKINEIGQAVV
                     LAGSGMCNGG"
BASE COUNT         4762 a         5141 c         5673 g         4613 t
ORIGIN      
        1 ttctggaacc tttgctcagt gaagcgaggc aacttcgcca ggaagggata tccggaaccc
       61 ttcctgtaag ccctgagtgc tatggatgta tggattgtgt cgccgcctgc ccaagggggg
      121 ctctaaggat agagaccctc gggatagctg gcaaaaaacg tgagtagtgg gtttgtaatt
      181 tgtgaatcgt gaatcgtgaa tcgtgaatcg aaaaagtcaa aagcaaaacc agatccttcg
      241 cttcgctcag gatgacagca gggtcaactg cgtggacggt gaacgggaag gggaaagacc
      301 tcccgacggg tagcaggggc aaattgtcaa tggtcaacgg taaaaaataa acagaagggg
      361 ccccgacaaa ggttttactt ggtccggccc cttctctgca agaataattg aaacctaaag
      421 gcccatgtag gcttttcgga cttcctttga atccagcagt tccttcgcgg ttccgtgttg
      481 aacgatcttg cctgtctgta tgacgtagcc cctgtcagct acttccaggg cttcctgaac
      541 catctgttct acaaggagga tcgtcactcc tgaacggttg atagaaacaa cggtttccat
      601 aactttttcc acgagggacg gcatcaggcc gagagaaagt tcgtcaagca gtagaagacg
      661 gggggccgac atcaggcctc ttgctatggc aaccatttgc tgttctcctc cgctcatggt
      721 ctcggccacc tgggagcgcc tgtcatataa tttcggaaaa agttcaaata cctgtttcag
      781 gcttttatct atggcattgc gatcgtttat cgtaaatgcc ccgagttcaa ggttttcctg
      841 gatagtcagt tttgaaaaaa gcctcctccc ttcggggaca taacttatgc cgagacggat
      901 cgtttcatgg gctttcatac gggaaatgtc ttttccctca aaatgaatgc tcccgctgaa
      961 ggggttcatc agcccggaga tcgtcctcat ggtggtcgtt ttccccgctc cgtttgcccc
     1021 gactatcgca accagttctc cctgggcaac ctcgagagag agcccgtgaa taacaggcac
     1081 ggagtcgtag ccgcaatgaa tgtcctggac cttcaggata gtgtcagcca tcactggctc
     1141 acctctttcg atcttttcag gcgctggctg aatttggcac ccaggtatgc ggtgatgacc
     1201 ctttcgtcct ccacgacatc ttcagggttg ccttcagaaa tcttttcccc gccgtcaagg
     1261 accactattt tgtcggcgac ggacataatg gcctccatga tgtgttcaac tacaagaagt
     1321 gtgattccgc gttttttcag gtttctgaga atttccacgg cggcctttat ctccgtagag
     1381 gtcagaccgg ccatcgcctc gtccagcatg agcagttctg gtccggtggc aagggcgcgc
     1441 gccatctcga gacgtttctt gaaaccaatt gtcagacctc cggcaagctg gccgctcact
     1501 tcctcaaggt gacaaagaga aatgcactct ctggccaccc tgttggccgt ttcggcatct
     1561 gcgccgcgaa ggaaagctcc tacaagaacg ttatcgagga cggtcatctc cttaagggga
     1621 tgaactacct ggaaagttcg cacagctccc ctgcgtgcga tttcgtaagc aggaaggctg
     1681 gtgacttcct cgccccggaa gaacatgttg ccggaggttg gtttgtagaa tcctgaaata
     1741 cagttgaaaa gtgttgtctt ccccgcgccg ttgggaccga tgagcccaac cacagatccc
     1801 ttttcgacgt cgaaggagac attgttgcat gccacgaggg atccgaacct catggtaacg
     1861 ttgcggatct caagaagggc cattctgttt gcccccttcc ctgtgctgtc tgatgctttt
     1921 cgtgaaacgg tcgaggatac ccattatgcc tttgggctga taaaccacaa ctatgatgat
     1981 cagaagacca aaaataatca ggtccacacc gccgccaagg ccgccaagat aagccctgga
     2041 atattcctgt aatggaatga gaagagccgc accaagcagg ggtccgtaga atgtgcccag
     2101 tccgccgaga atggttataa ggacgaactt cattgacata tcgagtgaca ttaccatcgg
     2161 agggtctaca cggtagttgt actgggcgaa gaatgccccg cagagtgcgg ccagaaaggc
     2221 agacagagcc atagctgaaa gtttgaccct tgtgctgttg acccccagtg attcggcggt
     2281 ctcttgtccc tccctcacag ccctcagata gaagcccagg cggtttttct cgatccatcg
     2341 tactattcca aagacaataa agaagaggag aaggaccccg tagaaataac cgaccttgga
     2401 ctggtaccaa acgaaatttt tccagccggg ctcggtaaac ccgtaatcaa ggcctatagc
     2461 acctccgacc caataccaga ccatgaacaa ccggttgaaa atttccacta ttgcgaaggt
     2521 ggcgatggcg aaatagtggc ctttcagttt gaagcaaggg taactgatag ctaatgcgac
     2581 caatgctgca accattgctc ctgcaaggat tcccggccaa ggtatcaggc cgaaccggac
     2641 tacggccatt cctgtgccgt atgctccgat cccgaaaaat acggagtggc cgaaggaagt
     2701 ctggccgcaa tatcctccca ggatgttcca ggcctgggcc atgctcgagt agagcagtac
     2761 ctggatcatt acgtgctggg caaaggagct gtggttgaac catggtgtca gtggtataac
     2821 agcaaatgct gccagaagac ccagccagaa cagatccttt cctgtcatct ttcctgaaat
     2881 tttcatggaa tcaccacccg aaaaggcccc tgggcctgac cattaccact acaaggtaga
     2941 gtccaaacac ggcgacgtat ttgaacaccg gtgctatgta aaaaccggca aatgcttcga
     3001 cgagcccaat aagcagggat gccagaaggg ctccgggaat gctgccgaat cctcccatgg
     3061 cgacacaaac aaaggcgata agactgaaga gcgcccccac ttcagggtgt accgcgaggt
     3121 aagaggggag aagaccgccc gcaattccca cgcaggcacc gccgagtccg aagacaagga
     3181 gatatatcct ttcggtgttg atccccatca attccgcggc ttccttgtcc attgccgtag
     3241 cctgtattgc ccacccgagc cttgtctttt taacaagcct gtagattacg aaaagtagag
     3301 ccagcgcgac gagcccaata acgagttggg ggacaggaat aataattccg gcaagatcta
     3361 tggttttccc tccgacaaac gtatcctgca gaagccggaa gttcggggaa aaacggttaa
     3421 ggcagatgtt cttgagcagc atggaaagcc cgaaagtcgc aagaagggcg gacattgcag
     3481 acttgccaat ggttctgctg ataatcagcc ggtaggtcag ggctcctact atgaaaacga
     3541 aggctccacc ggcgatggaa gagatcagcg gatcgatctt gaaaatgaaa ccaagccagt
     3601 aactcagata catagctatc atgagatatt cgccatgggc gaagttgatc acatccatga
     3661 cgccccatat cagactaagg cccgctgcca ccagcgcata gagtagcccg ttcaggagtc
     3721 cgtcaaaagc gacctggaga aacgtggcca ttcatgttac acccccagtt catcgatcat
     3781 ttcggtttat atcctttggt gcatcgaaac aagagaggca acagagaaac ccctgctgcc
     3841 tctctggcag atcctttaac cgatgttcaa agaatcgggt ccagtaaatc cagactgaaa
     3901 atgagattcc aggccaggat tagcgatctt tccagggaac catgggaata actggttcag
     3961 tatcggccag atctacaggg tagatcctct tgtactgtcc gccctggagc tgggtgagca
     4021 tgctcatggc cttgacattc tgtccgccgg gagcaaaggc gactttacca cccatggaga
     4081 gtggggattc ccactcgttg gcatggaggg tctcggcaac aactgtcggg tcaaggcttc
     4141 cggatttttc gatagcctgt gccagaacaa ggagcgcgac ggcttcctgg atggagtcgc
     4201 tgtcaaaggg gacgctgggg tcggtcttgg tcttgtagat ctcttcgaca gcggcaacgg
     4261 ctggcatcag ttcggcaaac tcaggggcat aaccggtgcc gcccataaag tagttggcgt
     4321 cgtctccgag ctgaccggcg atgatgggat cctggtagcc ggtgcagtag ttaagggcga
     4381 ccttagggag ccagtccatc tgcttcattg tgcggaccca aagggtatag tcgccgccaa
     4441 ggcatgcgcc gaaaacggca tcggggtttg cggccttaag cgtctggacc tcgctgttga
     4501 ggttggtggc gccggggttg aaggctacat ctgcaacgac cttgaaaccg ccagcttcgg
     4561 cagccatgcg tccctcttcg gcagcgtgtt tcccgaactc ggtgttctcg taaataaggc
     4621 caaccgtctt catgtcagtg tttttggttt tgttggccca ttcaatgaat tcgacaaatt
     4681 caagagattc ggttttgtca gttggagcca tgcggaagaa gtatttgaag tcgcgctcag
     4741 tcagggctcc tgagctggag cagccgcaca tgaaaacttt ctgtttcagt tccgcaacga
     4801 aacttgccgg ttttgtggca gcgctgttgt agcttccaat aataccccag accttttcct
     4861 ggtcgaaaag gcgctcggcc tcggattttg cgacatcggg tttcccctga tgatcagcat
     4921 gcacaaggac aaatttatag ccatcaagga ttcctttctg tgcagcgagg ggaacctcca
     4981 tttcggggtg cgcgttattt ataacctcgg ccgcggtctc gactgccgcc tggcaacgct
     5041 ggccagccag agcgcaaggg ccagtgagcg ggaaaagggt tcctatcttt attacctttt
     5101 cttcagcgaa agctgcacct gcgaaagtgc ccataaccaa aagcgctgcc agggccagta
     5161 acagaacctt gccaaaagcg ttttgcctca ttttctgatt cctccctctc aactataaaa
     5221 tcgtggccta cagactacat tgcaacccga ttctcattat cattattcta gtcctaaaat
     5281 tgggaaaagt taatagggaa ctgcaaaaag tgaccagaat ggacctgaag agcaggcgtc
     5341 aggaagctgg gaacaggaga ctggaagggc aagagcttgt cattctgagc gtagcgaagc
     5401 ggaatccgcc agcaggcgga ctttccgcac cctttgccca ccggcgggaa tctggttcag
     5461 accccgtgaa ttgtgaatcg tggaaccctt ttacccctgg gtaaccctgt ttcttcctga
     5521 ttccttgctt ttgtagagaa gagaatcggc cctcttcagc aggctttcag aagactcgcc
     5581 atcgatatga ctagtggcgc cgatggatat ggtcacgcac agcttttcgc cgttattggc
     5641 aagatatgag ttttctataa gcattcgaaa cctttcggcg accctgacga gttcatcagg
     5701 gtcagcgttg cggataacac ctacaaactc ctctccgccc catcggccgc aaacgtcaaa
     5761 gggcctgctg ttgtcagcaa gtgtccgggc cacgaatttc agtacctcgt cgccggtatc
     5821 atgtccgtag gtgtcgttga atttcttgaa atggtcgatg tccataaaaa ggacaccgaa
     5881 aggaactgca agacgatcct tttccgcaat acgtcccagt atctcttttt ccataaagcg
     5941 ccggttcggg atctgggtca ggttgtcgat catagccaat ttttccagtt ccgccaatct
     6001 tatcctgacg gcttcctttt cgcggagatc cgtgaatagc tctacggctc cgacaacctc
     6061 tcctttttcg ttcttcaggg ggctgaccct taccgagacc gggaccctgt gccctgcctt
     6121 gtggtgaagg aacacttcgt cctctcggtt ctgtccacct gaaataacgt ccgaaagggg
     6181 gcagttgccg gtgcagaggt tgttaccctc cgtgtcgacg tgggtcagaa tattgctgga
     6241 gcaggttctt ccaactactt ctccggcact gaaaccggtg atcagttccg cagccctgtt
     6301 ccagaaccgg ataaccctgt cgcggtcaac gatgtacagg ccgtcatgga gctggctcac
     6361 tatctcttca taaaactcct tgccgaatcc ggcgggtatt acattttctc tgctcaaatt
     6421 ttttcctcct taccgaccct ggtgaaacat tttttaacgg ggtcccattc ccatctttcc
     6481 aggacggtga tacccctggt gttgtcatac cattcaaagc aaatttctcc cgatccggtt
     6541 gcccggatct cttcgctgga ataattatgg tcgctgacga atattccccg cggcatgtcc
     6601 atgaaaggca tttccacggc ctctccagag ggaaggaacc ctatcaccct gtaaagattc
     6661 ccgtttgatc cggcgtgctg cccaaggttt gtttctggcc ttccatcccc tgtaaagtca
     6721 gcgaactgga gtgaccattc tccttccagg aagatcagtg gttcgtccgg gtattgggga
     6781 ttcatgatca tgttcaatgg tgtcgcgacg gcagttccat ctgccagatg ggctaggatc
     6841 tcgaaggttc cctcaaatct cgtccccatg aacggcccgg ggatgtcatc ctcgactatc
     6901 tcccccgatg tcatgttgat cgagacggcg acgatgggaa gaggtgtatc gatgaaggtc
     6961 tccgtaaata ccaccggcac gcctccagat gcgcaagatc tggtcatcag aaagaatatc
     7021 ggcaaaaaca gaagaacagg gcgggaaaat ctccccttcc atttcattat ccggtgccct
     7081 cccatctttt ctgaagttcc aataaccagg cttccctggg gcttcacttt attgcaagca
     7141 ggaaataaag aaccgggata attttaaccc ctttcgcctt tgaccaaaaa ggccaaaaag
     7201 taaaccctta tcataataaa gtatctttct ttatttctta ctcgcagttt aaaagacctg
     7261 tgggaaaaag gaaacccgaa aaatttcttt gacaagccgt taaacacagg ataaaattat
     7321 ttatgaacac aaaatcattt ttttgaaccc agtttcaaaa aggggttaaa aggtaaccgc
     7381 aagttagaaa aagtttatta gagagaggtg taaaaaaatg gcagtcaaca gttcaggaca
     7441 acttatggag atggagcacg agtacggagc gcacaactat cacccccttg aggtagtcat
     7501 ttcaaaagcc gaagggatct gggtggagga tcccgaagga cgtcgctaca tggatatgct
     7561 ttccgcctat tcggcggtga accagggtca caggcacccc aggataatca aggcgcttaa
     7621 ggatcaggcc gatatactca ccctgacctc aagggcattc ttcaacgaca gatggcctct
     7681 gttcgcaaag aaactggcct ctttcacagg gaaagagatg atccttccca tgaacaccgg
     7741 ggccgaggcg gtcgagacgg ccatcaagac tatgcgcaag tggggttacc tggtaaaggg
     7801 agtagaaaag gacaaggccg aaataataac ctttatggag aacttccacg ggcgcacggt
     7861 caccattatt tccttttcag tggacgaagt ggcaaaagac tattacggac cctacactcc
     7921 gggattcaaa gttgttccct acgatgatct gggtactgtg gagaaggcta ttaacaaaaa
     7981 caccgtaggg atactcgtcg aaccgatcca gggcgaagcc ggagtagttg taccgagtga
     8041 cggattcctc aagggtctgg aggatctttc ccggaagaac aaccttcttc tggcggtaga
     8101 cgaagtccag accggtttct gtcgcaccgg gaaaaacttc gcctaccagc acgaaggaat
     8161 cgaccccgat atcatcatca tgggcaaggc ccttggcggt ggggtattcc ccgtctcggc
     8221 catagcggcc aacaaggatg tccttggtgt cttcgaaccg ggcagtcatg gttcgacctt
     8281 tggcggcaac cctcttgcct gtgctgttgc ggccgaggca atagatgtac tggtcgatga
     8341 aaagcttgct gaaagggcca gagaactcgg cgagtatttt atggatggtc tcaaggccat
     8401 caagtccgac aagataaaga tggtcagagg caaggggctc cttatcggtg ttgtccttaa
     8461 cgaatcggca ggcaaggcga ggatctacac taccgccctc aaggacaggg gattgctctg
     8521 caaggagact cacgactgga tcatcaggtt cgctccgcca cttgttataa agaaggaaga
     8581 gatcgacaag gccctggagg ttattcggga ggtactgggt tagggaaaag acctggccta
     8641 ttttgtcgag ctgactgaat tgtttttttc aggggatccc gaaaacaggg gtcccctttt
     8701 ttggtctcaa tttttcaagg ggcatcgaaa aaatcatttt tgaagtatcc ttgaatcagg
     8761 aacatgacct ttcgaacatc tgaggaggag gttaaaccca tggagtacaa caaggtgctg
     8821 atcagcggga gcgaggatca tgttgcttac ctgaccctga acaggccgga aagaatgaat
     8881 tcctttgaca gcgagctggc ggcggaactt tacgaagctc tggccgaact tgattcttcc
     8941 gatgatgtcc gtgtaatagt gataaaagga tcggggaagc atttttgcgc cggcatagac
     9001 atttccgaac tggcgggtaa aagcgccatg gaataccggg actgggtaga gctgatggag
     9061 agacctcttt accatatttc aaagataaag aaacccgtca tcgcctgtgt gcatggagtc
     9121 gctgccgcga acggttgtgg tcttgccgcc tcagcggacc ttgttatcgc ttccgaggac
     9181 gcaaggatgg ggctcaccgc tatcaatctt ggcctcaact gtgtcggccc ggttctggcg
     9241 gtagtcaggt gtatcggcag aaaaaaggct ttggaattcc ttctttccgg agaacttttc
     9301 ggagccaggg aggctcaggc catgggcctg gtgaacaggg tcgtccctgc cgcggctctc
     9361 gaagaggaga ctcacaagtg ggcattatcc tttgcgaaca agagccccct ggccctccag
     9421 acggccaaga cggcctttta tgccgcagaa gacatggatt acgaaaaaca gttcgctttc
     9481 atgaacgaag tttttgcgag actctgtact tcgcgggacg ccgccgaagg tgtcgccgcc
     9541 ttccttgaaa aacgaactcc cgaatgggaa ggaagataga ccaggggtgg aggtggaaac
     9601 aagtggagga cacgcgccct gggtgggatg aatatttcat ttctatagcc aacgttgtct
     9661 ccagtagaag cacctgtctc aggagaaagg tcggggccgt catagtgagg gagcgccaga
     9721 tagtaagcac tggctataac ggggctccca agggcacgcc tcattgtctc gaaactggat
     9781 gcctcaggga aaaacatggc gttccttcag gcgagaggca cgagatatgc agggggtcac
     9841 acgcggagat caacgccata gcgcaggcgg ccgccatggg ctccgcaacg gctggttctg
     9901 tcatatactg tactcacgaa ccctgttcat tctgtaccaa ggccctaatc aacgcgggca
     9961 taaggagaat agtgttcgtg aaccactatc ccgaccccat tgcctccctt ataagggaag
    10021 aggctggtat tgaagttgcc tgtatcgaag aaaccccggc cacagaggat cagaggtgag
    10081 aatccttggt tattgagatc accggaagaa agaaaacagc cccaaagttc atgctggacg
    10141 acctggtggg acttgagccg atcaaggggg agctcaggaa gatcgaggcc atgttgtggc
    10201 tgaaccgcca caggagaagc ggtgaccttg gcgagctgac gcttcagtcc ttccatttca
    10261 gtttcagggg taatccggga acaggaaaga ccaccgtagc aaggcttata ggagatatct
    10321 ttcacagata tggccttctt agggtcggtg atgtggtaga agttgacagg tccaagttga
    10381 tcggaccgac tcccggagat acagagacca ggaccaggaa agcattgaaa agcgccctcg
    10441 acggtgtttt gttcatagac gaggcataca gtctcctcgg atccagagag agttccgacc
    10501 cggggataag ggccctggag gttatcctga aaggtatgga ggattacagg gatgccctga
    10561 tcgttgtatt tgccgggtat ccggtggaaa tggacgccct cttcgaccac gttacaggtt
    10621 tgaggagcag ggtgccctat cacctggaat ttccggatta ttccccaagg gaactggtgg
    10681 atattgcgat cttcatggcg gagtcagaga atctggctct ttcggacgaa gcttccgccg
    10741 cattcctggc gaaaatggag gagcgttcga gccttcccgg gttcagcaac gcaagagagg
    10801 tccggaacct catagaccag gcaaaggcca gcctttcggc acgcctccag acgagaagga
    10861 aagtttcccg gtgggacatg aaggttatca cttcagttga cctccaggat gaaggttccg
    10921 agagttttgc tcttctcgag caagccaaaa gggagatgta caggtcgccc tccgaacctg
    10981 gtgtccgttg tgcttacgcc agggcttgtt cgaatgccgg tctctggtcc gacgtggtgt
    11041 cggtgctcga acctgttcgt gattcgatcc cttcagagga cctttccctc tttggcaggg
    11101 cactgtacat catcggagaa agaaaacgtt catgggatgt cttttcgtgt ctgaaaactg
    11161 acgggtcctt tttccagggg ctctccgcga tgtggtccgg agaactccag gaggcttcca
    11221 gacttctcaa ggagtccgcg gcccaaaaca cgggtgatcc ggatatccaa cttgccctgg
    11281 cagcgggatg tttctttgag ggagattttt ccggatcggc aaggtccttt tccaggggac
    11341 tggaactttg cggctcaggt ttgccgtcac ttctcttgcg tgatctcccc tatgaagaac
    11401 tgcgatggaa ggaaagctcc cagaaccttg ccaaggccct tcatttcgcc tacgggaatc
    11461 ccgaccgggc cagactctgt tttacccggg ccctgatcga tacgggcgaa aagagtgccc
    11521 tcgtttctgc ctcggaactg gtctttgagt gcatcgacag aacccctgat gatccttctg
    11581 cccacaggat gatgtccctg ataaaggaat cggaaggtcg ccctggagag gccgccgctt
    11641 ctcttgaaat atcactagag atagagccgc gcaggaccga agactggaga agactggctc
    11701 ttctttacga gaaaacaggt cagaacgaaa aagcggagga gatcttttcc gatatcctcg
    11761 aggaggatcc taccggtgga gcaggtctcc gtcttgccgc ggcagccgaa acccggggtg
    11821 atctccaggg ggcaaatgaa ctttaccgca aagcctgggc tgctggcctt gcgggggaag
    11881 aacggtccat atgtgccgtg aaacttggtg tctattccgc agcggcgggg cgttttgccg
    11941 agggggccag attcctgatg gaagccggga accttgcaaa agaaccggaa ggggccttct
    12001 ggatggcaag agcgctcata gaggaagaaa aatggcagga tgccgagacg cttcttcttg
    12061 gtgtgccttc agggggaaat atgggaaatg ccagacttta ctggctatgt cgcacatata
    12121 tagcaagaag agatcttttc agcgcaaaaa acatccagtg gggcaatgac agcgaggcat
    12181 acacaagact tgccaggggt gtcaccgcgg ccctttcggg tgatccttcc cacgccgcac
    12241 tgctggaagg acttccggcg agagacatgg ggtgcgatgc cctgttcatg ctctgttcgg
    12301 ccagggcctc agtgaaagat tgggaggagg ctcgacacct cggaaacatg gcacagattt
    12361 ccgaatacgg acccttcctc tgggaacgtc agatcaaaag acccagggaa gaggccgcgt
    12421 atcttgcggc cattgcagag gcccatcttg gaaactgggc cgcggcgaga gaggccttcc
    12481 gaaggagcgc ctcggccctg aggcatccgg gccccgtctt tgcctttggc gtttccatgg
    12541 tcgctaccgg aaatatcgat gaagcccgta aaatttctac gcagatacag atgacttcac
    12601 cttccctttc acgcaaactg gatgaactca tcacccagaa cagcgggatc agaaaaatga
    12661 tggccgatcc ggtggacccg ggtattcttg atctttacgc ttttatctga tcggatcaaa
    12721 ttatcgggaa agaaaaatat atttgtcggg aggaaacaat tatggcagta ataacaagag
    12781 acgaagctat gaccatgctc agagagcaca acagcgaggt ggcgcatatc aggcatgccc
    12841 ttgccgttga agccgcgatg aggcattttg cgaaactcca tggagaagat gagggtaaat
    12901 gggctctggc tggtctggtg cacgacatcg actgggagac caccagcgaa gatccttcac
    12961 ggcacaccca tgtcggggca ggatggctca aggatgccgg ttatcctgat gacatagtaa
    13021 gggcggtcct tgcccacggg tggggaatat gctctgatgt tgagccattg tccgccatgg
    13081 agaaacatct ttacgccgtc gacgaactga cgggattcgt taccgcagtc gctctcgtgc
    13141 ggcctggcaa atccctgcag gacctgaccg tcaagtcggt gaagaaaaaa tggaaggaca
    13201 aggcctttgc aaggggagtg gacagggatg tcatagaaaa aggcacatcg atactcgaca
    13261 cgcccctgga agacctcata gagaacgtga tcgaggcact aaaacccgtg gaaagagaga
    13321 taggtctggg cgagggctgc tagcgcggca tttcggagtc gatgaggcgc cagaagggtt
    13381 ctttgtcctc agtaccggtt tcgaaacgca ggagcatgta ccctcctatg tgcccgtcct
    13441 cggcccaggc caggcaccag tttgggccga gaaaccaaac cgatgacggg tcgtagatcc
    13501 tcatcgtgcc gccaagaacc ccttcccagg ggataaggtc atccctggac agaatgcttt
    13561 ccgtcagcca tgtcttcggg tcgggcctct gactggtttt tattctttcc cagtcgggcc
    13621 ctggcgggaa atgtttcatg tttgaagact ccatgaccgc ccttacttta tcgagttctg
    13681 acttggcgac agagatctcc tccctcagct ccctgttctg gtcgacgact ttcgtgtatt
    13741 tccagaagac cgttccggat actgccgaaa gaagcagtat tatcaccact gccagcaagg
    13801 tcgacctgtc tttcattttc cgatccccct tccagccgtt ttactgcgat ttgtgatatt
    13861 gtctgatcgg gtcacatact gatagtttat cattgaaacg aaaaggagag agtgttttga
    13921 agaaactgtt cctcaaggtt ccggaactta ctgcaagatc ccttgttctt gcggcggccg
    13981 gttctgtttt gatagccgga agcagttcct acgttgctct caggatagga gcccttccct
    14041 ggccaacgat ctttgtggcc gttctgtcca tggccattct caagcccttc ggggccactc
    14101 tgagggagat aaacgttgcc cacaccggaa tgtcggccgg aggtctggtt gccggaggac
    14161 tggcgtttac ccttcccggg gtctggatgg cagatccggg ctcttctatg ggcgtctgga
    14221 ccgctgttgc ggcggcgctg gtcggtgcct ccctcggggt agtgttcaca ggtcttctca
    14281 ggaaacattt tatcgaggaa ctggacctcc cctttcccat gggagtggct gcggcccaga
    14341 cgctccaggc cggtgaccag ggtggcaaag gggccttgac cctgttcgct tcgtgtggat
    14401 ttgccggagt gattgcatac ctcagggatg ccgcaggtgt tcttccgccc ctcataggca
    14461 gggggcctct cttcagcgtc tggctttccc ccatggctgt cggtataggt ttcatcatag
    14521 ggccccttta catgggtaca tgggcggcag gagcaattct ggcccactgg tttatggttc
    14581 ctgtcgggct ggcatctggc tggttcactg acggggccac cgccgcggca ttcaggaaca
    14641 gcctcgggat aggtgtaata gtcggtgcgg gaattgcggt cctgttcagg ggcgcagcaa
    14701 agtcgtttct caggaccctt gctacctctg cgggcaacct tcgctttgcg gcggcggcct
    14761 cggccctttc ggcagtcgtg ctgacctttg cggcaggagt gcccctggtc ccttctcttc
    14821 tcgccactgc cggagtctgg ataacggttg taatggcagc gaccataacg gggcagacag
    14881 ggatagatcc catggaaata ttcgggatac tggtgcttct cgcggtcaga agcctttggg
    14941 atataggggg acaggaagcc tttctggtta ccgttgtcgt tgctgtagct accggtcttg
    15001 ccggtgatgc catgcaggat ttcagggccg gggctatcct cggaacggac cacagggccc
    15061 agctggtcag cgaggccgtc ggcggcattt ccggtgccgt ggcagcggcc tttactattt
    15121 ttcttattag ggaggccttc ggcgcgatgg gacccgggac cgatctggct gctccccagg
    15181 cttttgcggt caagagcatg atagaaggac taccagacag gggggccttc attcttggcc
    15241 ttctttccgg actttttctt acagtggcgg gagtgccgtg catgacggta ggtataggga
    15301 tgtacctgcc tgtagcaatt tctcttgccg ctggagccgg agggctgtca cggtttcttg
    15361 cgaacaggtt ctttcccggt tcgggcgaaa gattttccct tgtcgcttcc ggatttcttg
    15421 gcggagaggg tatagccgga gttctttacg ccatctggaa ggttgcgact ctgggatagg
    15481 gaggttattc aagtgtggcg gataggtcat gctcaggatc acgaaaaagg gaccggatgc
    15541 actgtcctgc tttttcctga aggggcgtgc gcgtcctgtg atgtgagagg cggagccccg
    15601 ggaacaaggg agacggatct tctagccccg gagtgtcttg tcgacaggat aacagcgata
    15661 gtcctttctg gcgggagcgc gagggggctt tcttcggccg atggagtaat gcgttattgc
    15721 gaggaaaagg ggtggggatt cgataccggg ttcggaatag tgccgatcgt tcccgccgcc
    15781 tgtctttttg atcttccatg tggggagagt ggagcctggc cggatatttt catggggcgc
    15841 gaggcctgcc tttctgccag agggctcgat gaaagcccca tgggcaatgt gggggccgga
    15901 acgggggcca cagtcgggaa atacgtcgga cttgacaagg cgatgaaatc cgggtttgga
    15961 tggagcaaaa agacatcggg atccctggag gtatcggcag tagtggcagt caatgctttc
    16021 ggtgctatca ggggcacatc cgggaatttc attgccggag cggttgacga gggcaggatc
    16081 attgacccct gctccctttt cgaaaaaggc gggatctgga gcgggtgggg caaaaacacc
    16141 accattgggg ccatagttac aaatgcctcc ctcgacaagg cccagtgcag gagagtggcg
    16201 ataatgggtc agactggcct tggagccacc atattccccg tcaactctcc ctttgacggc
    16261 gatacggttt tctgcgccgc gacatctgag acccatgccg agatgatcct cgtcggtgcc
    16321 ctcgccgccg atgctatggc agaggctgtc cggaacgcag tgaccgggac caggtcggct
    16381 ttcggtttgc ccgcaacagt cgatctcgaa gaaacaaatt ccgtaaacaa taggtaagcc
    16441 agcggatacg cgtttccctg ttacaggaga agatcaattg cggcaggggc tttttcctga
    16501 gagcctatcc ttcattcgga ggtgtcccaa tgactatcag agtgatcctg gcagatgacc
    16561 acccgctgac ccgcaagggc ctgaaggact acatagaaaa agaatctgac cttgatcttg
    16621 taggggaagc ctccgatggc acgggggcct gggaactgat cggcaaggaa cttcccgatg
    16681 tggcccttct ggacataagg atgccgggag agaacggagt ctcccttgcc aggaggatca
    16741 gggattcggg cctcaaggtc ataccggtca tgctgacttc ctttgattcc caacagtatg
    16801 ttattgcttc cctgcgggcg ggggccaggg ggtatatcct gaaaacggta actcctgatg
    16861 aactgtcaag ggcgataagg atagtggcga aaggcggact ctatctcgat ggagaggtcg
    16921 cccggagcat ggaggagagc gactccgcct ttgaatcgct ttcggccagg gaaagggagg
    16981 tcctccttta tgcggcaagg ggactttcca gcaaggagat cgcccgtgac ctcataatca
    17041 gcgaacggac cgtccagacc catctcgggt cgatctacga caagcttggt gccaggaaca
    17101 agaccgaggc aatgctgctt gccctgaaat acggcgtagt ggctcttgag gaactaattg
    17161 aatgaggaga catctcctca tttttctgac acttgtaata atgatcccct cattttcggc
    17221 ccttcttata ggggggatcg gccttgtcca gcatgagagg acaatggaaa gggtggccag
    17281 gtcctatgtg caggatatgg ccgaaagcgt tgcctccaga atagagatgg gctggaacag
    17341 gaccaggacc tctcctctga gcgaccggga gagattcctt cgcctcaggc agataacatg
    17401 gggtctttcc atccccggat ggatggccgt agttgatgtc cacggcaata ttcttctttc
    17461 ctcctcggga gccgaggtgc ttcctctcat ctgggacagg ggaattcctg taggttcagc
    17521 catggaggtc gacgcaaaag acggagacag gtataccctt gccgcctacc cggcagggga
    17581 gacgggatgg tacgtagtga tcgccgtatc ctgggacaaa cttctcggcc ccatgctgag
    17641 attcagcagg tggccggtaa tggtgggtct gataggcctt ctgggtcttg tttctgtctt
    17701 tgctctctgg aagtggctcg ttgctcccct gcgtatcctg gggaacgagg tatcctcttt
    17761 gcaatggggg cgtgatatgc ctgtggccga ccaccccggc gctgttttcg aaataagacg
    17821 tctccggcag gttctgcatc agctggcgag ctccgctgta gacagggcag aactggttaa
    17881 aagatatgtt ggagatcttg tgagggttca ggaggaggag cgttcccgta tcgccaggga
    17941 gatccatgat gggcccttac aggatgtcac tgcgctgatc caccagatca ggctggccct
    18001 tatggagtca ttgcccgatg gtcgcgacag ggaacacctt gataatgcgg aggagggtgc
    18061 ccgccacgct gtcagggaga tgagatccct gtgtgatgaa ctatccccgc catggctgga
    18121 tctgggactt gggcaggccc tcgaggaact gggggaaagg ctttcaagat acctgcaggt
    18181 tgagatcgtc gtagaggtgg actgttgcga ggatcttccc cccgaggtca ctctggcctt
    18241 tttcagggta gttcaggaag cggtacacaa ctccgcccgc cacggcaagg cttcccgtgt
    18301 agcggtatcg ctgggtaagg ctgaagacgg ggtgatactg gatatagagg acaatggaac
    18361 cggattcgac gccaacgtca actacgagga actcagggtc agggggcaca ggggtctcgc
    18421 caacatggcc gaaagaatga ccctcatagg cggaaccctt ctggttgaaa agggaagaga
    18481 agggggcacc agggtgaggt gcatgctgcc agttacttcc gtttctgtct gaagtaccct
    18541 ccccataccc ccctgacatc ggaagtaaag agaaatcctt tggtgccaac gatccgggcc
    18601 acgtggttga tatcatttct ctggcagatc accttcagta tcagtctttc tcctgtcttg
    18661 ccggagcccg aaacaacggt ggtgccgtag ccttcttcac ggaggaggtc aactatcccg
    18721 aagatctcct cagttacggg ggccacgacc tccacaagaa caaaagagct gagaattcgc
    18781 tcttccagag atgctcccag gtaactgcct acggcgaaac cgccggcata ggcaaggagt
    18841 tgaaggggtc ctgttatgcc ttcctggagg acatatccaa gggcaataat ataaacggca
    18901 gattcgaaaa aggctgttat tccggcctgc atctttttac ccctgaccag aaggagaatc
    18961 ctgaaagttc caagagaaac gtccagaagc ctggcggcaa atataagaag tgcaccgaat
    19021 ggatccatgt gaaagacctc cctttggata ctgggaattt gctcatgccg gtaccatccc
    19081 gtcatgagat tctagtatag aatgtaagca attgaaagaa aaatcgaaag ggggggagcc
    19141 cccccagggg gcaaactgat gagacttcgt gttcttggag cggccggaga ggttaccggc
    19201 tccagttata ttctcgaggc aggaccgtca aggataatcg tagatttcgg gcttcaccag
    19261 ggaggtaacg aaggaaagaa cagcgagccc ctggagttcg atccgaaaag cgtcgacgct
    19321 gtcctgttaa cccatgctca tctcgatcac acgggaaggg tgccccttct tgtaaaaaaa
    19381 ggtttcagcg gaaaggttat tgctacggct cccacggtgg agcttacgga tgtcatctgg
    19441 gccgactcgg ccaagatcca gaaggaggat gccgagtgga aaagccggaa gaacagcagg
    19501 aaaggacttc cccccgagga acccctttat gacgatgatg atgtagagaa ggcagtcaag
    19561 atgcttgtgc cggtgaccta cgacgagata attgagccgg ctcccggttt caaggtaagg
    19621 ttcagggatg ccggtcatat tctcggcagt tccaacctgg agatatggat gaacgataac
    19681 ggggaggaga tcaagatcgt cttttctggt gatcttggcc cccagaaaac cgtcatggag
    19741 aggaacccat cggtcataga ggatgcagat tacgtggtca tcgaatccac ctacggcaac
    19801 agagagcaca ggacaaacga ggaaagccgc caggagttcc gggaagcgat gaaggaaatg
    19861 ctcagtgaca aaggcaaggt tctgatcccg acctttgttg ttgacagggc ccagagagtt
    19921 ctttacgaac tgatgctcat gcagatcgag ggaatacttc cctcgaatgt tccaatttat
    19981 tttgattccc cgatgggggc taaagccacg gcagtctaca cgaaatacat ctcccttctt
    20041 tcttcggaga tacagtcgca ggtgcgcagc ggcaacgacc ccttttctcc ggagaacctg
    20101 aatatagtat cgagtgttga agaatcaaaa aagataaacg agataggtca ggctgtcgtt
    20161 ctcgccggga gcggaatgtg taatggggg
//
LOCUS       DAOTIQ010000033        20102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3236_length_20102_cov_6.628373, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000033 DAOTIQ010000000
VERSION     DAOTIQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 20102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20102
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3236_length_20102_cov_6.628373"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5541_05585"
     CDS             complement(<1..214)
                     /locus_tag="P5541_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HRV71488.1"
                     /translation="MSDYSFDLLPERKGTCALKWDGMNDRFGPVPKDALPMWVADMDF
                     LAPSFIMEAIRNRASEGVFGYAQAPLS"
     gene            complement(242..637)
                     /locus_tag="P5541_05590"
     CDS             complement(242..637)
                     /locus_tag="P5541_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRV71489.1"
                     /translation="MTGLFLKNYFFTLIPFVALDAVWLGLVAPKFYRSQIGYIMAERP
                     NWFAAAAFYLLFIAGMVFFVTGREGSISQAVLRGAVFGFICYATYDLTNLATLKGWPI
                     LVTVVDMTWGTVLGGGTALLAVWLKGFFS"
     gene            779..1552
                     /locus_tag="P5541_05595"
     CDS             779..1552
                     /locus_tag="P5541_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71490.1"
                     /translation="MPLRLLRLLPLLSRLLAGKRRELAKGDNGPAQPEKTLDNPDIQK
                     TGPFSRGEAIEFQGFDKKIVHSEDIFSGFLFVLFTSGVVLTGIIAASVPSDPDPLTES
                     GLSVVTLLFFVFLFLAINFSSLRIRMTFDHLTARYGVFSYRADWLAVKDIIIERNNSG
                     ASPNRGIRMRSIGEGTWRLYYSTGLPRVILVFEDGAIRELAFSSNSPEKAMTAARECI
                     RRKKARYESAAHKGEVHPGSGPEIPRPLHLAKQKSGKNQ"
     gene            1549..2322
                     /locus_tag="P5541_05600"
     CDS             1549..2322
                     /locus_tag="P5541_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71491.1"
                     /translation="MNRLTLFIISTMLAISFLFCPDSAALARTYDELAEQYQSIARYR
                     DLVAEFEITLLEKAQQLSEKELWLFVTEMENHAGQRASNGLSLIRKIFPEGDPANWHE
                     ISGFFMPVEISRPLAALDAVYFTCMALVSMDKPEAAWLARQLLSSLRNSSRGAFLAIR
                     TAPKEYLFLTSRISASTGPAPVGGWPQAEITGQLPFARPPSSFTTQEYAIMKNMIFLN
                     ASGQPVPGTGPYAWDRKNGRIHRVTESRDDLQWWWLQGR"
     gene            2444..3808
                     /locus_tag="P5541_05605"
     CDS             2444..3808
                     /locus_tag="P5541_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV71492.1"
                     /translation="MSKDREYMLANDPVGKLIMNLSLPAVVGMFVNGLYNLVDTIYIG
                     HGVGSLGIAALSVSFPIQMIIGGTGAMLGIGTASLISRSLGAKDYETAQHAFGNNLLL
                     IGVTGVIYVLIGTVFLPRILSLFGASPEILPYASEYMGIVFIGSPLIIFAMSMNNVIR
                     SEGAAKIAMWSMLVGAIANIFLDPIFIFGLGMGIRGAAIATVLSRILVIAWIGRFFLS
                     GKSIISFRRTDMVPRLRVLQKILVVGFPALLHHGSSSFVFGLINQILGFYGGNIAVAV
                     FGVNNRIIIFSAMPIIGIAQGMQPIVGYNYGATQYRRTLEAIATSNRIALAFCSGISL
                     LLLAFPVYALRVFTSDPAMLAIGPQAMRLMVAGFFLAGYNKVAGSVFQALGKAFPAFL
                     INTARPILFFVPLLMVMPRFIGINGVWLSFAVADVLSFALTVCFLVPETRKLRSLEPL
                     PEGA"
     gene            3816..4301
                     /locus_tag="P5541_05610"
     CDS             3816..4301
                     /locus_tag="P5541_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRV71493.1"
                     /translation="MDGTAGKLLSIIHRNLCSFMDRSFSDCDIGHGPRRFLVEISFFP
                     GHTQEEISERLLMDKTTTARAVKQLELRGYITRTRDPEDRRRYRLQTTTKGDEMIPQV
                     LEALEFAQNTLYEGLNQKELADAARILRKMADNALNLRERTFCRLSEKQDSQPTGKCG
                     K"
     gene            complement(4230..5813)
                     /locus_tag="P5541_05615"
     CDS             complement(4230..5813)
                     /locus_tag="P5541_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71494.1"
                     /translation="MYCSCRPPCPDGALFREECCRQCDRKTRRTILTAAIIASSLSFI
                     DLSAVNLALPAIQKDLSASAAHIQWIIQLPALFLASLLLIGGSLGDHFGLKKVLAWGL
                     GIFVISSTGCAMAGGIRSLLFFRATLGIGGAIIIPASFSLISYFFEIDERGKAIGIWS
                     GTTGLTVVIGQVLGGAMTTFISWRTIFLLPIPLVAIVYERMRSIPPAPPVSKDRQGGF
                     IEAFSLFVFLGAIVLIFIELPQRGFADSMIFSLMIVIAVAGSVFLLSVRKGANHIASV
                     DMYTLPGLAGANVITFVLYGSFTGSLFFLPLYLVQIQGFTEVQAGLALSPFVIVIFLL
                     ARVGGFLTDRKGPVTPILSGCILIAFSFLLLARAGIRAEYIKDLLAPVIIMGSGFALV
                     SPAISTAVMNSVQRQNLGAAAGLNTTVSRVAGLAGLALMGLIMTISFKHSFAAELVSA
                     GVRPSLSETLLPLAGDLGGMSVPEDLGQEIAIIVVGSRDLAFVFGFRVLCITSALLSA
                     FSGWLAILFFRKPAKRSFS"
     gene            complement(6062..7750)
                     /locus_tag="P5541_05620"
     CDS             complement(6062..7750)
                     /locus_tag="P5541_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71495.1"
                     /translation="MRSIKCYALAMIIALLMVLSSPLRSDCSPEAMTVAPDPVGVAND
                     FITGLTGSAPAALGLASDGSEILSIGQDILKGDHKGALSRLGLFLSEKSVGTVPVIGQ
                     FYAIGKLGKTLGDMTMKWAGEKNFNEIFESVREAAVTDSAGGWPRNLEAAKRDDFFYL
                     VFNERFKVFDTYLEKKTGIKDPEARENLAIEMILSRGRMEDLFDQYGLTGKNRTYEKL
                     QMEIEMEAEIEARFALRKEEERIAAILDREKDNGAEEPSAKPGIDQKKVSLPEPEMPE
                     SYPQQQAEKTIPQPAEEADPSPVAVAKGEPEKEKNETQESKVAGGPLSWSIDASTDGP
                     ETTVFRVSITNISDSLLEDIVHDVQPLNEVVSGGVGFGTGSKAMVLLPGASGQFTVLS
                     MGDTEGVLITLRGKGKTLATITAMSVHETKEKKSKGEEQVYFQGTFWDLKGNLKGTIN
                     LIINGRAVTGDLSGSYRDHEQNLTISASIEGAYDPEKAMISCSFSGRAIGKAKFEMPD
                     QYDMETQDIDVNEIISGSIRGIHGTVGLSGSWVGASSFIDLEGRWEAKPGLTGK"
     gene            complement(7758..8891)
                     /locus_tag="P5541_05625"
     CDS             complement(7758..8891)
                     /locus_tag="P5541_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV71496.1"
                     /translation="MIARKIFSAAAVLFFLTNPAVIFADEAQPFFDRAMRYVSQQDFE
                     RAVISFERALEERPGYAEAHYNLGVALEALGRTEEAVSHYESTLSLMPEYAGAMNNLG
                     NAYMALGQTEKAREMLQRAVDTDPSMTIAWYNLGNVLLEQEKVEEAAKKYTRVIELEP
                     KAAKAHFQLGRIYTKLGRFKEAIESYERTLDLSPGSKDIMFNLSAAYGASGDREKEID
                     ILLELVDLDPENSLVFYNLGTSLFRVGRFRESVEYLKRSTELEPSYTHSWNNLSQSLL
                     RLKDYPAAVEAAREAVRTRPDFAMAHASLGFALLGMELFQDAKTSFAESIRLLPEYAE
                     PRFGLAIAALALGDNETALEQKQILEGLNPQLAEKLAQIISKQ"
     gene            complement(8931..9797)
                     /gene="folD"
                     /locus_tag="P5541_05630"
     CDS             complement(8931..9797)
                     /gene="folD"
                     /locus_tag="P5541_05630"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRV71497.1"
                     /translation="MSTDREKNIARILDGKAVAASVRNDIKNRVRELEKIGKTPPGLA
                     VVLVGDDPASKVYVGQKEKACLEVGFRSFLHRLPASASGGDVLSLVDSLNRDAKVHGI
                     LVQLPLPGQIDETFVLEAIDPSKDVDGFHPANMGRLVMGLPSVEPCTPRGIMHLLSEY
                     RIETSGKKAVVLGRSNIVGKPVAHMLMAADATVTVCHSRTPELETYTREADIIVAAIG
                     RPSFLKPHMVKEGCVVVDVGINRLENGLVGDVDFKGISEKASWITPVPGGVGPMTIAM
                     LMLNTFERYLQN"
     gene            complement(9815..11494)
                     /locus_tag="P5541_05635"
     CDS             complement(9815..11494)
                     /locus_tag="P5541_05635"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRV71498.1"
                     /translation="MINKVPSDIEIAQNAPIRPITEIAAKLGIGGEDLVMYGPYKAKL
                     TRGVWERIKGNRDGKLILVTAITPTPAGEGKTTTTVGLGQGLAAKGKKAAIALREPSL
                     GPSFGVKGGAAGGGYSQVIPMEDINLHFTGDIHAVTTAHNLVSAMIDNHLQQGNDLGL
                     DPRRIVWRRVLDLNERALRNVIVGLGGKAHGVPRESGFDITVASELMAILCLSTDLMD
                     LKERVGRIIIGYTYDNRPVTAKEIGAAGSVAVLMKDALKPNLVQTLEGVPAFVHGGPF
                     ANIAHGCNSVQATRHGLKLADYFVTEAGFGADLGAEKFFDIKCRMAGLKPDAVVLVAT
                     VRALKMHGGLPKTELTSENLDALRAGLPNMEKHIENLNSFGVPVVVAVNRFPTDSDAE
                     LELVRSSCEVLGASVSLSEVWEKGGKGGVDLADKVIEACSEKSTFHYLYEADETPRQK
                     IEKIAKNIYGAGDVQYTDVALKDLDNISSMGLDDLLVCMAKTQASISDDPAKTGRPDG
                     FTLTVREVRVSAGAGFIVPITGSIMTMPGLPKKPAALGIDIDAEERITGLF"
     gene            complement(11605..12936)
                     /locus_tag="P5541_05640"
     CDS             complement(11605..12936)
                     /locus_tag="P5541_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV71499.1"
                     /translation="METGIAVSLVLFLFLILAGLPLGWVFLASTATGLILMGSRLTFM
                     AGTFFHSVFTPVVMAIGFFVFAGALISEGGLADRIVRFSYALVGKAKGGLIVVGIVAT
                     LFMSALTGSSVPCISALIPLLVPRLEKYGYMKKYTTAVLCSSSFLGYLIPPSVPALIY
                     CLVAQQSVAALFLATVFPGLLLALGYGILNYFIVDRYTFPTDEKPDLPDNFKDSMKEL
                     LSATWTALPALGCPAIILVGIYGGIFTPNEAGAIAVIYCMVVGFFIYRDLKIGNFARA
                     VTSTLVTLGMIALLLGGGTVLTRLLTREGVAQQLASMMLGLFESKFMIMISINLFLLL
                     LGMFLDGIPILILAVPLILPLARELNVNLVHLGSIIIVNIGLGVMTPPFAMSIFVGSR
                     LSGVPAGELIKPMMLFLLCVGIPVLLMTTYIPFLSCWLPTLALGQQVVGLW"
     gene            complement(12936..13460)
                     /locus_tag="P5541_05645"
     CDS             complement(12936..13460)
                     /locus_tag="P5541_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV71500.1"
                     /translation="MLNFINRQLGTLEKWICSVGLLVSTFLVFAQVLNRYWLHYEIMW
                     IGDLALYIFVFSYISAIAFATFRRGHISVEMFKTKFLKDRPMGLAVYDLVMDTLSLVV
                     VLIFSIPVREFFLRSLKYPEYGTLVRWFNQGWLIYALFGVVILSSIHLLLQIKEDISE
                     ITRLRRVDPEGGSQ"
     gene            complement(13558..14637)
                     /locus_tag="P5541_05650"
     CDS             complement(13558..14637)
                     /locus_tag="P5541_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV71501.1"
                     /translation="MQKKTGFRRFICFALVVGLFMALSAAPSMAKSAEYKWKFAQTTV
                     RPTQAISMRLFCDLVKKYTDGRVEIKFYENGIMGSHNEIFHGVQQGDIEIGNFAPYVN
                     LVPGGYFNWMPWTISSWEESEIAFGRPNGVLYKVLQDAYDEVGMYVLYTNSQGPYGLG
                     NNVREIRKPEDLTNLKFRVSSSLGSVMGLVNMGKGTGMTVETVDWGELYNALSRGVVD
                     GCWSMWPSLIEERHYEVLKYYTSLDWMWDGNQIVINKELWNSLPKDIQDAVVKAAVEA
                     EAHQYKLSRAAVESMQEQLKNSDGFNIYYPTEAEKEVFRKKADMLTVWNELCKPWLDE
                     KYPGQNMTEKILAELDAIRVKVHGK"
     gene            complement(14753..16354)
                     /locus_tag="P5541_05655"
     CDS             complement(14753..16354)
                     /locus_tag="P5541_05655"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRV71502.1"
                     /translation="MSLDVIIRGGTVFDGSGAKGEIADIGIEGDVITAVGNLADVEGV
                     DTIDARGRVVCPGFIDTHSHSGIGVLADPASAHNTMQGITTCLIGQDGMGAAPIVDEY
                     KEPWKKSMAGLEGSYDIEWDWKSVREYLKRLDDAGTGPNFAYLAPHSNIRLSVMGLND
                     RDPSEEELKKMRNLLEKCIEEGSFGMSTGMIYPPCSYAKTAEFIKMGKVLKDTDTLFV
                     THQRSEADTILDSMDEIIQIGKESGCRIHFSHFKVAGKKNWDKFPGIMEKLDQCGKEG
                     INVSVDQYPYVAGSTILSVLLPPWAHDGGTDKILERLGDPEARKRMTRDILGGIHGWD
                     NFVDFAGLDGIFITFVKTSRNSDAVGKSLVELGEMRGKEPLEAVYDLLLEEENVAGLV
                     DFYGHEDHVRQIMRRPEQNFCTDGILGGKPHPRVYGSYPRVLGKYVRDEKVLDMATAI
                     NKMTGKPASVLGIRDRGLIKKGLAADIVVFDPRTVIDRGDYSDPVRFPSGIDHVLVNG
                     KSVVRDGSHVPAKAGKVIRYAGRKA"
     gene            complement(16436..17899)
                     /locus_tag="P5541_05660"
     CDS             complement(16436..17899)
                     /locus_tag="P5541_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV71503.1"
                     /translation="MIDLAMNDEQLKRTVERARERNIIIPTFEQMKNPEKIPAKIKER
                     LKKVGLWDVDSTNLFRITWKNEPVKSGGLHGKVNFLELPKEITGVDARIVALVGKWFP
                     TGSHKVGPTVGCLVPRLVTGQFDPTTQKAIWPSTGNYCRGGAYNSQLLACESIAILPE
                     GMSRERFDWLKQVAGEVIATPGSESNVKEIFDKTWELKRTRNDVVAFNQFEEFGNYLW
                     HHEVTGSAVEEGFRLVARPGDRFFGVTFTSGSGGTLAAGDYMKKIFPNSKIAVGEALQ
                     CPTLLQNGFGAHRIEGIGDKHVPWIHNVKNTDMVIDVDDADSMALIRLFNEPAGKAYL
                     MSKGVSREMVDQLPLMGISSAANVIMAIKMAKYYELTGKDVIFTVFTDSMEMYGSRLK
                     EMEEEYGDYSETDAAVDYNRSILGLGTDWMKELTYYDRKTIHNLKYYTWIEQQERELD
                     ELNAQWYDAENYWGSIPSLAAKIDEKIDRFNQMTGLL"
     gene            complement(17925..19181)
                     /gene="glyA"
                     /locus_tag="P5541_05665"
     CDS             complement(17925..19181)
                     /gene="glyA"
                     /locus_tag="P5541_05665"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRV71504.1"
                     /translation="MFGKGSECLRKVDSAVAAIIDAEMKREREGIELIASENVVSPAV
                     LAAMGSVLTNKYAEGYPRKRYYGGCEVVDQAEDLARERAKELFGCDHANVQPHSGSQA
                     NMGVYFSVLKPGDTILAMSLSHGGHLTHGSPVNFSGQLYNVVSYGVSKETETIDFDQV
                     ASLAREHKPKMIVCGGSAYPRTIDAVKFREIAKDVGALLLFDIAHIAGLIATGNHPDP
                     MPYCDFVTTTTHKTLRGPRGGMIMCTAEFAAAVDKAIFPGMQGGPLMHVIASKAVAFE
                     EALQPSFRDYQKQVVTNAKALADSLLKRGFRLVSGGTDNHLILVDLTERGVTGKDLEI
                     ALDKAGITVNKNTVPSEKRSPFVTSGIRIGTPAVTTRGFREPEMDLLAGWIDRTARGV
                     EDEKELAAVRAEVLELCGKFPLYEGF"
     gene            complement(19213..>20102)
                     /gene="thrC"
                     /locus_tag="P5541_05670"
     CDS             complement(19213..>20102)
                     /gene="thrC"
                     /locus_tag="P5541_05670"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRV71505.1"
                     /translation="VGVAKALDFGQKTVACASTGNAASSLSGFAAVSGLKSYIFVPAS
                     APSAKVTQLLVYGSAVVLVKGDYQAAFDLATEAIDKWGWYNRNCAINPFLIEGKKTVA
                     LEIAEQLEWDIPDVVMVSVGDGCIISGVFKGFSDLLELGITDRIPRIIGVQAQGASPI
                     FSAVEKGLERVQFGPADTVADSISVGAPRNWAKALRAVRKSGGKMVAVTDGEILEAIP
                     ELARETGVFAEPAGAASFAGLKKAAMQGDIGQGDRVAFIVTGNGLKDIPSAQRSVAAP
                     FEIAPDIGELARLPGLATD"
BASE COUNT         4849 a         5537 c         4787 g         4929 t
ORIGIN      
        1 ttgacaaagg cgcctgggca taaccgaata caccttccga agccctgttc cgtatcgcct
       61 ccattatgaa tgaaggggca aggaagtcca tgtccgcgac ccacatgggg agagcatctt
      121 tgggaacggg gccaaaacgg tcgttcattc catcccactt cagcgcacag gtgccttttc
      181 gttccggaag aagatcaaaa gaatagtccg acaaaggtat cacatccgtt cccaagttta
      241 ttcaactgaa aaaacccttg agccagaccg ctaaaagggc cgtgccccct cccaggacag
      301 taccccaggt catatccact acggttacga ggatcggcca gcctttcagg gtggccaggt
      361 tagtaaggtc gtatgtggca taacaaataa aaccaaaaac cgcacccctc aggacagcct
      421 gcgaaatact gccctccctg cctgttacaa agaagaccat acccgcaatg aagaggaggt
      481 aaaaggctgc cgccgcaaac cagttgggcc tctctgccat tatgtaaccg atctgggatc
      541 tgtagaattt tggtgccacc aaacccagcc atacagcatc cagagcgacg aacggaatca
      601 gagtaaagaa ataattcttc aggaaaagtc cggtcatttt cattcacctc cattggtcgt
      661 atttcccctg ataccattct acgacaaaca agggttcacc ggcattccct catacccggg
      721 aatagtgtat cctgataaaa aggcacataa acatagatcc caaaaagggg gaacaattat
      781 gcccctcagg cttttaaggc ttttacccct tctctcaagg cttttagccg gtaaaagaag
      841 ggagctggcg aaaggcgaca atggcccggc tcagccggaa aagacccttg ataacccaga
      901 tatccaaaaa actggccctt tctccagagg cgaagcaatt gaatttcagg gatttgacaa
      961 aaagattgtc cattccgagg atattttttc gggttttctc tttgtcctgt tcacttcagg
     1021 agtcgtcctg acaggcatta ttgccgcatc tgtcccttca gacccggatc ctttgaccga
     1081 gtccggtctc tctgtggtaa cactgttgtt ctttgtcttt ctgtttctcg caatcaattt
     1141 cagcagcctc cgaatccgga tgacctttga ccatctgact gcaaggtacg gtgtttttag
     1201 ctaccgggcc gactggcttg ctgttaaaga cataataatc gaaagaaata acagcggggc
     1261 gtcaccaaac cgcggaatac ggatgagatc cattggtgaa ggaacatggc ggctttacta
     1321 cagcacaggc cttccgaggg tgatactggt ttttgaggat ggcgctattc gggaactggc
     1381 tttcagcagc aacagtccgg agaaggcgat gaccgcggca agggagtgta tcagacgcaa
     1441 aaaagcgaga tatgaatcgg ccgcacataa gggagaggtt catcctggat ccgggcccga
     1501 aatacctcgc cctcttcacc ttgcaaaaca gaaaagtggc aaaaaccaat gaatcgcctc
     1561 acattattca ttatctcaac gatgctggcg atatcgtttc ttttttgtcc ggattcggcc
     1621 gcactcgccc ggacttacga cgaattggcc gagcaatacc agtcaattgc cagatatagg
     1681 gatcttgtcg ccgaatttga gatcaccctt ctcgaaaagg cccaacaatt gagcgaaaag
     1741 gaactctggc ttttcgtaac agaaatggaa aaccacgccg gtcaaagagc atcaaacggt
     1801 ctctccctca tcagaaagat cttcccggaa ggagatcccg ccaattggca tgaaatctcc
     1861 ggttttttta tgcccgttga gatttccagg cccctcgccg ccctggatgc ggtgtatttc
     1921 acctgcatgg cccttgtctc aatggacaaa cctgaagcag cctggctggc ccggcagctt
     1981 ttgtccagcc tccgcaattc ttccagaggt gccttccttg caattcgaac ggccccgaaa
     2041 gaatacttgt ttctgacatc cagaataagc gcatcaacgg gtccagctcc cgtgggaggc
     2101 tggcctcagg cagaaataac cggccagctt cctttcgccc gtcctccatc ctcattcaca
     2161 actcaggaat acgcaataat gaaaaatatg atattcctta atgcatcagg ccagcctgtg
     2221 ccgggaacgg gaccctatgc ctgggacaga aagaacggcc gcatacaccg tgtgacagaa
     2281 tcaagggacg accttcaatg gtggtggcta cagggaagat agatcccctt taaataatac
     2341 ttccggttca acctttggag ggagaaaaga ctttaaaagg ttgcatatac aaccgattta
     2401 tactatgatc cctccgttcc tgaaataaga acggagggtt tttttgagta aagaccgtga
     2461 gtacatgctg gcgaatgatc ccgtgggaaa acttatcatg aacctttcac tacctgccgt
     2521 cgtgggaatg ttcgtcaacg ggctctacaa ccttgtcgat accatctaca tcggccatgg
     2581 cgtgggttcc cttgggatag ccgctctttc agtctccttc ccaatacaga tgatcatcgg
     2641 tggaaccggg gcaatgctgg gcatcggcac cgcctctctg atatcgcgca gcctgggagc
     2701 aaaggattat gagactgccc agcacgcctt tggcaacaac cttctgttga tcggtgtgac
     2761 cggtgtcata tacgtcctta tcggaacggt ctttcttccc cggatacttt cccttttcgg
     2821 agcatcccct gagatacttc cctacgcctc cgaatacatg gggatagttt tcatcggcag
     2881 cccccttatc atcttcgcca tgtccatgaa caacgtcatc agatcggaag gggcagcaaa
     2941 aatagccatg tggtcaatgc tggtcggagc tatcgccaat attttcctgg atccgatctt
     3001 tatattcggt ctaggaatgg gcatacgcgg ggcggcaata gcgacggttc tttccagaat
     3061 ccttgtcata gcctggatcg gacgattctt tctctctggg aaaagcatca tttctttcag
     3121 gcgcacggac atggttcccc gcttgcgtgt cttgcagaag atcctggtag ttggattccc
     3181 ggcccttctt caccatggtt cctcaagttt tgttttcggt ctgataaacc agatacttgg
     3241 tttttacggg gggaatatag cggtagctgt attcggcgtg aacaatcgca tcattatttt
     3301 ctcagccatg ccaattatcg gaatagccca ggggatgcag cccatcgtcg gttacaatta
     3361 cggagccacc caataccgac gaacactgga agcaatagcc acttccaacc gcatcgctct
     3421 tgctttctgt tcgggcattt ctctcttgct cctggctttc ccggtttatg ccctgagagt
     3481 attcacctcc gatcccgcca tgctcgctat tggacctcaa gcaatgaggc taatggtggc
     3541 cggcttcttc cttgcgggct acaacaaggt ggcaggaagt gttttccagg ctctcgggaa
     3601 ggcatttccc gcattcctta taaacactgc cagaccgata ctctttttcg tccctcttct
     3661 catggtcatg ccccgtttta tcgggattaa cggcgtatgg ctctcattcg ccgttgcgga
     3721 tgttctttct ttcgctctga ctgtctgctt tcttgtccca gagacccgga aattgaggtc
     3781 ccttgaaccc cttccggaag gggcttgatc gtaaaatgga cggaaccgca gggaaattgc
     3841 tttcgattat ccaccggaat ctttgttcgt tcatggacag atccttcagc gactgcgata
     3901 tcggacacgg cccaagacgt tttctggtag aaatttcttt tttcccggga catacacagg
     3961 aggagatcag cgagaggctt cttatggaca agaccactac tgcaagggca gttaaacaac
     4021 tggaattgcg gggctatatt actcgtaccc gtgaccccga agaccggaga cgctaccgcc
     4081 tgcaaactac caccaaaggg gacgaaatga tcccccaggt tctcgaagca ctcgaatttg
     4141 cacaaaatac cctttatgaa gggctaaacc aaaaggaact ggctgacgcg gcaagaattc
     4201 tccggaagat ggctgacaac gctctgaatc taagagaaag aacgttttgc cggctttctg
     4261 aaaaacagga tagccagcca accggaaaat gcggaaagta gtgctgacgt tatacacagc
     4321 accctgaacc cgaagacaaa agcgaggtcc ctggaaccaa caacgataat cgcaatttcc
     4381 tgccccaggt cttccggcac ggacatgccc ccaaggtctc cggcaagggg aaggagtgtc
     4441 tctgacaagg aaggccgaac ccccgcagag acaagttcag ccgcgaaaga atgcttgaaa
     4501 gatatggtca ttataaggcc catcagcgca aggccggcaa gacccgcgac ccgggaaact
     4561 gtagtattca gacccgcggc cgccccgaga ttctgtcttt gaacagagtt cattacggcc
     4621 gttgaaatag cgggcgaaac gagagcaaac cctgaaccca ttataatgac tggcgccaga
     4681 aggtccttaa tgtattcagc cctgatgccg gcacgggcga ggagaaggaa agaaaaagcg
     4741 atcagaatac aaccggagag gatgggcgta accgggccct tcctgtcggt aaggaaccct
     4801 cctacccttg caagaaggaa tatcacaata acgaaagggg aaagagcaag tcccgcctgg
     4861 acctcggtga aaccctgtat ctgtacgaga tacaggggga gaaaaaagag agatcccgta
     4921 aaggatccgt atagaacgaa ggttattacg ttggctcccg caagacctgg tagtgtatac
     4981 atatcaacac tggcaatatg gtttgctcct tttctgacgg agaggagaaa aacagaacca
     5041 gccacagcga taacgatcat taaagaaaat atcatagagt cagcaaatcc tctttgaggc
     5101 agttcaataa aaatcaagac aattgctccg agaaaaacaa aaagagaaaa agcttcgata
     5161 aaacctccct gcctgtcttt tgataccgga ggggccggtg gaatgcttct cattctttcg
     5221 taaacgatcg ccaccagagg aatgggtaaa agaaatattg ttctccacga aatgaacgtc
     5281 gtcatcgctc cccccagcac ctgaccgata accactgtca gccccgtcgt acccgaccat
     5341 attcctattg cttttcctct ttcgtcgatc tcgaaaaaat aactaataag agaaaagctg
     5401 gcgggaataa tgatcgctcc accgatcccc agggtagcac ggaaaaaaag aagagatctg
     5461 atccctcctg ccatcgcgca tccggtagat gatataacga agatacccag gccccaggcc
     5521 aggactttct tgagcccgaa atgatcacca aggctccctc cgatcaggag cagggatgcc
     5581 aggaacaggg caggcaactg tattatccac tgaatatgag ccgcagaagc cgagaggtct
     5641 ttttgaatcg cgggaagtgc caggtttacc gccgaaagat ctatgaaaga caggctcgaa
     5701 gctataatag cggccgtaag tattgtcctt cttgtctttc ggtcacactg cctgcagcac
     5761 tcttcgcgga agagcgcacc atcagggcag ggaggtcgac aggagcagta catttcatca
     5821 cccctcaggg ttacaatgta tcctgtgcaa tctcaggcac tttattgttt catgatcttt
     5881 catgaggagt cccgtccgaa catgatagca cttgtaataa tatccctggc tgcaatggca
     5941 ataaccatat tgtctttcag aagttcccgt caatgcccga aaggttgcca cgggtgcggt
     6001 caatgcgcac ggatcaaccg ccagatcagg aaagaaacag aagaatcccc atttcctccg
     6061 actacttgcc ggtcagacca ggctttgcct cccatctgcc ctccaggtca atgaaactgc
     6121 tcgcccccac ccaactcccg gatagaccga ccgtaccatg gattcccctt atgctcccgg
     6181 agataatctc attaacatca atatcttgtg tttccatatc atattggtcg ggcatctcaa
     6241 attttgcctt tcctatcgcc ctgccagaaa aagaacatga gatcatggcc ttctccggat
     6301 catatgctcc ttctatagat gcactgatgg tcaggttctg ttcgtgatcc cggtatgaac
     6361 cggaaaggtc accggtaacc gcacggccat ttatgatcaa attgatggtt cctttcaggt
     6421 tccccttgag atcccaaaaa gtcccctgga aatatacctg ttcttctccc ttgcttttct
     6481 tttcctttgt ttcatgaaca gacattgcag ttatagtggc aagggttttg cctttaccac
     6541 gaagggttat aagaacccct tcggtatcgc ccatggacag gaccgtaaat tgtccggaag
     6601 cacccggaag aagaaccatc gctttagatc cggtgccaaa tccgactcct ccagacacca
     6661 cttcgttgag aggttgaacg tcgtgcacga tgtcctcgag aagagaatcg gagatgttgg
     6721 ttatactaac tcggaacacg gtggtttccg ggccatcagt ggaggcatca atactccagg
     6781 aaaggggccc gccagcaacc ttgctctcct gggtctcatt tttttctttt tccggttcac
     6841 ccttggcaac cgctacagga gaaggatccg cttcttctgc aggttggggt attgttttct
     6901 ctgcctgctg ctgtgggtag ctttccggca tttccggttc cggcaatgat acctttttct
     6961 ggtcaatccc gggtttggcg gaaggctctt ccgccccgtt atccttttct cgatccagga
     7021 ttgccgcaat cctttcctcc tcttttctga gggcgaaacg ggcctctatc tcagcctcca
     7081 tctctatttc catttgcagc ttctcgtatg tcctgttttt tcccgtgaga ccatattgat
     7141 caaaaagatc ttccatccgg cccctcgaga gtatcatctc tatagccaga ttctccctgg
     7201 cttccggatc ctttattccg gtttttttct ccaggtaggt atcaaagacc ttgaaacgct
     7261 cattgaagac gagatagaaa aaatcgtctc ttttcgctgc ttccagatta cggggccacc
     7321 cgccagcaga atctgtaacc gctgcttccc tgacagattc aaaaatctcg ttgaaattct
     7381 tctcgcccgc ccatttcatg gtcatgtcgc ccagagtctt gcccaacttc cctatcgcgt
     7441 agaattgtcc tattaccgga accgtcccga ctgatttctc ggaaaggaaa agacccagtc
     7501 tggaaagagc ccccttgtga tcacccttga ggatgtcctg tccgatcgaa aggatctctg
     7561 agccgtccga ggcaagaccg agggccgcag gggcagaacc agtcaggccg gtaataaaat
     7621 catttgccac gccaaccgga tccggggcaa ctgtcattgc ttccggagaa caatctgaac
     7681 gcaaagggga cgaaagaacc atgagcaggg caataatcat cgcaagggca taacatttta
     7741 ttgatctcat atctgcctca ctgtttcgag atgatctggg ccagtttttc ggccagttga
     7801 ggattcagcc cttcaagaat ctgcttctgc tccagggctg tctcgttatc accgagggct
     7861 aacgccgcta tggccagacc gaagcggggc tcggcatact ccggcaaaag acgtatggat
     7921 tccgcgaagg aggtttttgc atcctggaac agttccatgc caagcaacgc aaaaccaagg
     7981 cttgcatggg ccatggcaaa atcaggcctc gttctgacag cctctctggc agcctcaaca
     8041 gctgcaggat agtctttaag tctcagaagt gactggctga ggttgttcca tgaatgagtg
     8101 taggatggct ccagttccgt tgatctcttc aggtattcca cagattccct gaagcgtcca
     8161 accctgaaca gagaggtccc gaggttgtaa aatacgaggc tgttttcggg atcaagatcg
     8221 acaagttcaa gaaggatgtc aatctccttt tcccgatccc ctgatgcccc gtacgcggcg
     8281 ctgaggttga acatgatatc cttgcttccg ggagacagat ccagagtcct ctcgtaggat
     8341 tcgatcgctt ccttgaaccg gccaagtttc gtgtatatcc ttcccaactg gaaatgtgcc
     8401 ttcgcggctt tcggctccag ttctatcacc cgtgtgtatt ttttagcggc ttcttcaacc
     8461 ttctcctgct ccagtagaac attcccaagg ttgtaccagg caatcgtcat tgatggatca
     8521 gtatctacgg ctcgctgcag catctcccgg gctttttcgg tctggcccaa agccatgtag
     8581 gcattaccca gattgttcat ggccccggca tattcaggca tcagtgagag ggtcgattcg
     8641 tagtgggaga ccgcctcttc tgtccttccg agagcctcca gggccactcc aaggttatag
     8701 tgagcctcgg cataaccggg tctctcctca agagcgcgtt caaaggatat tacagccctc
     8761 tcgaaatcct gctgcgaaac atacctcatc gccctgtcaa aaaaaggctg ggcttcgtcc
     8821 gcaaaaatga cggccgggtt tgtcagaaaa aacaagaccg cggccgccga aaaaattttt
     8881 ctcgctatca tggctctcca cctccgggat ggatctgaat cgccattcta tcagttttgg
     8941 agatatcttt caaaggtatt gagcatcagc attgctatgg tcatggggcc tacaccaccg
     9001 ggaacagggg ttatccagga tgccttttcg cttattccct taaaatcgac atctccgaca
     9061 aggccgtttt cgagcctgtt gatccccacg tcaacaacta cgcacccttc cttgaccata
     9121 tgaggtttca ggaaggatgg acgtccgatc gcggcgacta tgatgtcggc ttccctggtg
     9181 tacgtctcaa gttcaggggt tctgctgtgg catacggtga cggtggcatc ggcagccata
     9241 agcatgtgtg caaccggttt gcccactata ttgctccttc ccagaaccac tgcctttttc
     9301 ccggaagtct cgatccggta ctcactcaac aggtgcatta tccctctcgg ggtacagggc
     9361 tccaccgacg ggagacccat caccagccgg cccatgttgg cggggtggaa tccatcgacg
     9421 tccttggacg ggtcgatagc ttccagaaca aatgtttcgt cgatctggcc cgggagcggg
     9481 agctgaacca gtattccgtg gactttcgcg tctcggttca gggaatccac aagagagagt
     9541 acatctccgc cactggctga agcaggcaac ctgtggagga aggatctgaa tcccacctcc
     9601 aggcaggcct tctctttttg tccaacatat acttttgagg caggatcatc tcccacaagg
     9661 acaacggcaa ggccgggggg agttttgcca attttttcaa gttccctgac tctgtttttg
     9721 atgtcattcc ttacactagc ggcaacagcc ttcccgtcca ggatacgggc aatgtttttt
     9781 tctctatcag ttgacatggt cagattaacc gagcctagaa aagacccgtg atcctctctt
     9841 cggcatctat gtctatgccc agggctgcag gttttttcgg aagacccggc atagtcatta
     9901 tgcttcctgt tattggaact ataaaacctg ctccggcaga gaccctgact tctctaacgg
     9961 taagagtgaa accgtcaggt cttcctgttt ttgcggggtc gtcagatatg gaagcctggg
    10021 tcttcgccat acacacaagc agatcgtcaa gacccatgga agaaatgtta tccaggtcct
    10081 tcagggcaac atctgtgtac tggacatctc cggcaccata aatatttttc gcaatctttt
    10141 ctatcttctg ccggggcgtt tcgtccgcct cgtagagata atgaaaggta ctcttttccg
    10201 aacaggcttc gatcacctta tcagccaggt ccacaccgcc cttgccacct ttctcccata
    10261 cttcagaaag ggagacagag gcacccagga cctcacagct ggatctgaca agttcgagtt
    10321 ccgcgtccga atccgtgggg aacctgttga cggccacgac aaccggaacg ccgaaggaat
    10381 tgagattttc gatatgcttc tccatgttgg gaagccccgc ccgaagagca tcaaggtttt
    10441 ctgatgtgag ttcggttttt ggaagaccac catgcatctt gagtgccctt acggtcgcga
    10501 ccagtactac cgcatcgggc ttcagacctg ccatcctgca cttgatgtcg aaaaatttct
    10561 ccgccccaag gtcggctcca aaaccggctt cggtgacgaa gtaatcggca agtttcagac
    10621 catgccgtgt ggcctgaaca ctgttgcatc cgtgagcgat gttagcgaag ggaccaccat
    10681 gaacgaaggc agggaccccc tccagggtct ggaccagatt gggcttcagt gcatccttca
    10741 taagaacagc cacggaacca gccgcaccga tctcctttgc tgttacaggt ctgttgtcat
    10801 aggtataacc tattattatc cttccgaccc tctctttaag gtccataaga tctgtggaaa
    10861 gacaaagtat cgccatgagt tccgaagcaa cggtaatatc aaagccgctc tccctgggaa
    10921 caccgtgagc ttttccgcca agtccgacaa taacgttccg caaggctctt tcgttcaggt
    10981 caagaaccct tctccagact attcttctag gatcgaggcc aagatcgttc ccctgctgca
    11041 ggtggttgtc aatcatcgca gagacaaggt tatgggcagt cgttacggca tggatgtccc
    11101 cagtgaagtg aaggtttata tcctccatgg gaataacctg ggaataaccg ccgcccgcag
    11161 ccccgccctt gactccgaaa ctcgggccaa gacttggttc cctgagggca atagcggcct
    11221 tcttcccttt tgccgcaagt ccctgcccaa gaccgaccgt tgtcgtggtc ttcccctcac
    11281 ccgcaggggt cggagtaatg gccgtgacca ggatcaactt gccatccctg tttcccttga
    11341 tcctttccca tactcccctg gtcagtttgg ccttgtacgg accatacatc accagatctt
    11401 ccccaccaat cccaagtttg gccgcgatct ccgttatagg ccttattggt gcattctgcg
    11461 cgatctcaat atccgaaggg accttgttta tcattcccaa tttggctgga cctccctctc
    11521 tctgacgtct ggaaaacctg aatttcagga attatatatc ctttccgttt ttgtacaaaa
    11581 taaagcttta cataacgaag ttacttacca tagacccact acctgctgtc ccagagccag
    11641 ggtcggaagc cagcatgaca gaaaggggat ataggttgtc atgagaagga ccggaatacc
    11701 aacgcaaaga aggaaaagca tcatgggctt gataagttcc cctgccggga cacccgaaag
    11761 cctggacccg acaaaaatag acatggcaaa tggcggcgtc attactccaa gtccgatgtt
    11821 tacgattatg atcgaaccca gatgcaccag gttcacgttc agttccctgg ccagcggcaa
    11881 aataagagga accgcaagaa taagaattgg tatgccatca agaaacattc caaggagcag
    11941 aaggaacagg ttgatggaga tcatgatcat gaacttgctc tcaaagagac ccagcatcat
    12001 gcttgcaagt tgctgcgcaa caccttcacg ggtaagcagc cttgtaagga cggtaccgcc
    12061 gccaagcagg agcgcgatca tacccaatgt aacaagtgta cttgttacgg ctctcgcgaa
    12121 attgccgatc ttgagatccc tgtagatgaa aaatcccacg accatacagt aaataacggc
    12181 tatcgctcca gcctcattag gggtaaatat gcctccatag atgcctacga ggatgatggc
    12241 aggacacccg agagccggaa gtgcagtcca ggttgcggaa agcagttcct tcatggaatc
    12301 cttgaagttg tcgggcaaat ccggcttctc atcggtaggg aaggtgtacc tgtccacgat
    12361 gaaatagttg agaatcccgt aacccagcgc cagaagaagc ccgggaaaaa ccgtcgcaag
    12421 gaaaagggcc gccaccgact gctgggcgac aaggcagtag ataagtgcag ggacgctggg
    12481 aggtataagg taccccagga aactggatga gcaaaggacg gcagttgtgt atttcttcat
    12541 atacccgtat ttttcaagcc tgggaaccag gaggggtata agagccgaaa tgcacggcac
    12601 cgaggaaccc gtcagggcac tcatgaatag ggtcgcgaca atacccacca ctatcagccc
    12661 gccttttgcc ttgccgacaa gggcgtagga gaacctcact atcctgtccg caagaccccc
    12721 ctctgaaata agggccccgg cgaagacgaa aaatccaatg gccatgacga ccggcgtaaa
    12781 tacggagtgg aaaaatgttc ctgccatgaa cgtcagcctc gaccccatca ggatgaggcc
    12841 tgttgcggtg ctggcaagga aaacccagcc aaggggcaga cctgccagga tcaggaaaag
    12901 gaaaagaaca agggatacgg caattccggt ctccattact gactgcctcc ttctggatcc
    12961 accctgcgga gcctggtgat ttccgaaatg tcttccttta tctggagcag aaggtggatg
    13021 gaagacagga tcaccactcc aaacagggcg tatatcaacc agccctggtt aaaccaacgg
    13081 acgagtgttc catattcggg gtacttcagg gacctgagga agaattccct tacaggaatc
    13141 gaaaagatca gcactactac cagggaaaga gtgtccatta caagatcgta aactgcaagg
    13201 cccatcggcc tgtctttaag aaatttggtc ttgaacattt caacggatat atgtcccctg
    13261 cgaaaagtgg caaaagcgat ggcggaaata tatgaaaaaa caaagatgta aagtgccaga
    13321 tctcctatcc acatgatctc gtagtgaagc cagtatctgt taaggacctg cgcaaacaca
    13381 aggaaggtgg agacaagaag acctaccgag cagatccatt tttccaacgt accaagttgt
    13441 ctgttaataa aattcagcac caagcccaca ctccttgaaa gatccataaa ctggaggatc
    13501 gtagccgaaa gggctgccgc cgaaaaaaga cggcagcccg ctaaatttac tggatattta
    13561 tttgccgtgt acctttaccc ggatcgcatc aagttccgca aggatcttct cggtcatgtt
    13621 ctgaccgggg tatttttcgt caagccaggg tttgcaaagt tcgttccaga cagtaagcat
    13681 gtctgctttc ttcctgaaga cttctttttc tgcctcagtc ggatagtaga tattgaaccc
    13741 gtcagagttt ttcagctgtt cctgcatgct ctcgacagcc gccctggaaa gcttgtactg
    13801 atgagcctcc gcctcaaccg ctgccttgac tacagcatcc tggatatctt tggggagtga
    13861 gttccagagt tccttgttga taactatctg gttgccgtcc cacatccagt cgagagaggt
    13921 gtaatacttc aggacttcat aatgcctctc ctcgatgaga gatggccaca tgctccagca
    13981 tccgtcaaca acaccgcggc ttagggcatt gtaaagttcg ccccagtcaa cggtctcaac
    14041 ggtcatcccg gtgcctttgc ccatgtttac caggcccatg acgctaccaa gagatgagga
    14101 gacacggaat ttaaggttgg tgagatcttc cggtttgcgg atttccctta cattgttgcc
    14161 aagtccatag gggccctggc tgttggtgta aaggacatac atgccaactt cgtcataagc
    14221 atcctgaaga accttgtaaa gaacaccgtt cggcctgccg aaagcgatct ctgattcttc
    14281 ccatgaggat atggtccatg gcatccagtt gaaataaccg cccggcacca ggttcacata
    14341 gggagcaaag ttgccgatct cgatgtcgcc ctgctgcacg ccatggaaaa tctcgttgtg
    14401 gctacccata ataccgttct catagaactt gatctccacg cggccatcgg tatatttttt
    14461 gactaggtcg caaaacaggc gcatcgaaat ggcctgagta gggcgaaccg ttgtctgggc
    14521 aaatttccac ttgtattctg cgctctttgc cattgacgga gccgccgaaa gcgccatgaa
    14581 aagaccgaca acaagagcaa aacagatgaa gcgcctgaat cccgtctttt tctgcatgat
    14641 caaacctccc aggatgtttt gaaaaatcca agtaaacttt taccgcgcgt ttctttcaca
    14701 aaaagaggta ttttggggct tccctatcgg cacctccttc ctctgcccgg ttttacgcct
    14761 ttcttccggc gtaccttatg acctttccgg ccttggccgg gacatgagat ccatccctta
    14821 ctacactttt gccattcacc aggacatggt ctattcccga tgggaacctg accggatcgg
    14881 agtaatctcc cctgtctatc actgttctcg gatcaaaaac aacgatatca gcggcaagac
    14941 ctttctttat cagtcccctg tccctgatac ccagcacaga ggcgggcttc ccggtcattt
    15001 tattaatggc cgtcgccata tcaagtacct tttcgtccct gacgtatttc cccaggaccc
    15061 ggggataact tccgtatacg cggggatggg gtttgccccc aagaattccg tcggtgcaga
    15121 agttctgttc cggcctccgc ataatttgcc tcacgtggtc ttcatgccca tagaagtcga
    15181 ccagacccgc cacgttctcc tcctccagga ggagatcgta caccgcctcg agaggctcct
    15241 tgcccctcat ctctcccagt tccacaaggc ttttcccgac ggcatcgctg tttctggatg
    15301 tcttcacaaa ggtgatgaat attccatcga gacctgcaaa atccacgaaa ttgtcccatc
    15361 cgtgtatccc gccaagaatg tcccgggtca ttctctttct cgcctccggg tcgccgagac
    15421 gttcaaggat cttgtccgtt ccgccatcat gtgcccatgg agggagtaga acgctgagga
    15481 tcgtgctgcc ggcaacatag gggtactgat caacggatac gtttatccct tcttttccac
    15541 actgatcgag tttctccatg ataccgggga atttatccca gttctttttt ccggcaacct
    15601 tgaaatgcga aaaatggatc cggcatccgc tctccttgcc tatctgaata atctcatcca
    15661 tcgagtcgag aattgtgtcg gcctcgcttc tctggtgagt tacaaaaaga gtatccgtat
    15721 ctttcaggac tttccccatc tttataaatt cagccgtttt ggcgtaactg caggggggat
    15781 atatcatacc agtggacata ccgaaagaac cctcttcgat gcatttctcc aggagattcc
    15841 tcatcttttt caactcttct tctgaagggt cccggtcgtt gagccccata actgatagtc
    15901 tgatgttgct gtgaggagca agataggcaa agtttggtcc cgtaccagcg tcatcgagtc
    15961 tcttcaggta ttcccttacg cttttccagt cccattcgat gtcataactt ccctcaaggc
    16021 ctgccatgga ttttttccat ggctccttgt actcatccac gatcggagcc gcacccattc
    16081 cgtcctgccc gatcaggcat gttgtgatcc cctgcatcgt gttatgagca gaggcgggat
    16141 cagccagaac ccctatcccg gagtggctgt gggtatcaat gaagccgggg cagaccaccc
    16201 ttccccttgc gtcgatggta tctacgcctt ccacatcagc aagattgcca acagcagtta
    16261 taacgtcacc ctcgattccg atgtcagcaa tttcgccttt cgctccgctt ccatcgaaaa
    16321 cagtacctcc cctgataatc acatccagag acaatacgtt cactcccctc ttccaggccc
    16381 gtacagcagg atcacaaaaa ggcccgggga ggttcccccc cccaggcccg acaaactaca
    16441 atagtcccgt catctggttg aacctgtcga ttttctcgtc gatcttcgca gcaagggacg
    16501 ggatgctccc ccaataattt tcggcatcgt accattgggc gttgagttcg tccagttcgc
    16561 gttcctgctg ttctatccag gtgtagtact tgaggttgtg tatcgttttc ctgtcgtaat
    16621 aggtcagttc cttcatccag tcagtgccga ggccaaggat gctgcggtta taatccactg
    16681 cggcatctgt ttcactgtaa tccccatatt cctcttccat ctctttgagc cttgaaccgt
    16741 acatctccat ggagtccgtg aaaacggtga aaatcacatc tttccccgtc agttcgtagt
    16801 acttggccat cttgatagcc ataataacgt tagcggcgct ggagatcccc ataaggggga
    16861 gctggtcgac catctcccgt gatacccctt ttgacatcag gtaggctttc ccggccggct
    16921 cgttgaacag gcgtatcaaa gccatgctgt cagcatcatc gacgtcaatt accatgtccg
    16981 tgttcttgac attgtggatc cacgggacat gcttgtcccc gatgccctct atccggtgag
    17041 cgccaaaacc gttctgcaga agggtggggc actgaagggc ctccccgacg gcaattttcg
    17101 agttcgggaa gatctttttc atgtaatccc ccgccgccag agtgccaccc gaaccggagg
    17161 taaacgtaac accgaagaat ctgtctccag gcctggcaac cagacggaat ccctcttcca
    17221 cggccgaacc ggttacttca tggtgccaga ggtagttgcc gaactcctca aactggttga
    17281 aggccaccac atcattcctg gtccgtttga gttcccatgt cttgtcgaaa atctccttga
    17341 cgttgctttc acttcccgga gtggctatga cttctcctgc aacctgcttg agccagtcaa
    17401 agcgctccct gctcatacct tcgggaagaa tggcaatgga ctcgcaggca agaagttggg
    17461 agttgtacgc ccctccacgg cagtagttgc cggtggaggg ccatatagcc ttctgggtag
    17521 tggggtcgaa ctgacctgta acgagccttg gaacaaggca tccaacggtt gggccaacct
    17581 tgtgggaacc tgtgggaaac catttcccga caagagctac gatcctggca tcaacgccgg
    17641 tgatctcctt aggcaattca aggaagttga cttttccgtg aaggccgccg ctcttgactg
    17701 gttcgttctt ccatgtgata cggaaaaggt ttgtggaatc tacgtcccag agcccgacct
    17761 ttttgagtct ctccttgatt ttagccggaa tcttttccgg gtttttcatc tgttcgaagg
    17821 ttggaatgat tatatttctc tccctggctc tttcaacagt ccttttgagc tgttcgtcat
    17881 tcattgcaag atcgatcatc gtttgcatct ccttcgttgt attctcaaaa cccctcgtat
    17941 agggggaatt tgccgcaaag ctcgagaacc tcggctctga ccgctgcgag ttccttttcg
    18001 tcttcgaccc cacgggccgt cctgtctatc cagcctgcga gaaggtccat ctcaggctcc
    18061 ctgaaacccc tggtggttac ggccggggta ccgatcctga taccgctggt gacaaaggga
    18121 ctgcgttttt ccgaaggaac ggtgttcttg ttcacggtga tccctgcctt gtccagagcg
    18181 atctcgaggt ccttgccggt cacacctctc tcggtcaggt ccacaaggat aaggtggttg
    18241 tccgtgccac ccgaaacgag gcgaaaacca cgtttcaaaa gcgaatcagc aagagctttt
    18301 gcgttggtta ctacctgctt ctggtaatcc ctgaaagacg gttgaagtgc ctcttcaaaa
    18361 gcgacggcct tcgaggctat gacgtgcatc agggggcctc cctgcatccc gggaaatatt
    18421 gccttgtcga ctgccgcggc gaactcggca gtacacatta tcattccgcc cctggggccc
    18481 ctgagggttt tgtgtgtggt ggtcgtcaca aaatcacagt atggcatcgg atccggatga
    18541 ttacccgtgg ctataagacc agctatatga gcaatatcaa agagaagaag ggctccaaca
    18601 tcctttgcga tctcccggaa ttttacggca tcaattgtcc ttgggtaggc gcttcctccg
    18661 caaacaatca tttttggctt gtgttctctg gccagagatg cgacctgatc gaaatcgatc
    18721 gtctctgtct cctttgacac tccataggaa acgacattgt aaagttgccc tgagaagttc
    18781 accgggcttc cgtgggtaag gtggcctcca tgggaaaggc tcatcgccag gatcgtgtct
    18841 ccgggcttga ggaccgagaa atagacgccc atgttggcct gtgatccgga gtggggctgg
    18901 acgtttgcat ggtcacaccc aaacagttct tttgccctct ctctcgccag gtcttcggcc
    18961 tggtcaacaa cttcacagcc tccgtagtac ctcttgcgag gatatccctc cgcatacttg
    19021 ttcgtaagaa ccgatcccat cgccgcaaga acagcgggag atactacgtt ctccgaagct
    19081 ataagctcta tcccttctct ctccctcttc atttccgcat cgattatcgc tgcgacagcc
    19141 gaatcgactt tacgaagaca ttcgctccct tttccgaaca tgacgatctc ccctctcgat
    19201 gtttcccgtt ttttagtctg ttgccaatcc aggcaacctt gcaagttctc cgatatccgg
    19261 cgcgatttca aagggagcag caaccgatct ctgtgctgag gggatgtctt tcagtccgtt
    19321 tccggtaaca atgaaggcta ccctgtcacc ctgcccgata tcaccctgca ttgccgcctt
    19381 ttttaatccc gcgaaagagg cggctccagc cggttcggca aaaaccccgg tctccctcgc
    19441 gagttccgga atggcttcca ggatctcacc atccgttaca gccaccattt tccctccgct
    19501 cttgcgaact gcccttagag ccttggccca gttacgggga gctcccaccg atatgctgtc
    19561 ggctacagta tccgccggac caaactgaac cctttccagc cccttttcaa cggcactgaa
    19621 aattggtgat gcaccctgcg cctgaactcc tatgatcctg gggatcctgt cggtaattcc
    19681 cagttccagg aggtcggaaa aacccttgaa gactccactg ataatgcatc cgtcgccgac
    19741 cgataccatt accacgtcgg ggatatccca ttcaagttgt tcggcaatct ccagggctac
    19801 ggtctttttg ccctctatga ggaagggatt gatggcacag ttgcggttgt accaacccca
    19861 cttgtctatt gcctccgtag cgagatcgaa tgccgcctgg taatctcctt tcacgagaac
    19921 aacggcggaa ccgtatacaa gaagttgggt cacctttgct gacggggccg acgctggcac
    19981 aaaaatgtaa ctctttagtc ccgaaaccgc cgcgaaaccc gaaagagagc tggcggcatt
    20041 gccggtggaa gcacaggcca cggtcttctg tccaaaatca agggccttgg caacgcctac
    20101 cg
//
LOCUS       DAOTIQ010000034        20030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3253_length_20030_cov_7.713342, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000034 DAOTIQ010000000
VERSION     DAOTIQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_20030_cov_7.713342"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /gene="flgK"
                     /locus_tag="P5541_05675"
     CDS             complement(<1..1676)
                     /gene="flgK"
                     /locus_tag="P5541_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRV71506.1"
                     /translation="MSGTFSGLEIGRRALDYFRQGIETAGHNISNADVEGYSRQRVEA
                     APADPYAAVGFNSPGSPGQVGTGVEVSAISRIRDSFLDAQFRQESIEGGYWEALSRTM
                     EYLEMFVCEPSGTGLSASLDNFVQALHEFQKRPDSGPAREVLLREAENLSSLMGQISG
                     NFSEYRDSLNTEIELRVGEANSLIDRIAALSGQISRVQAMGNSPNDLMDRRDLLVEQL
                     ARLVDVSVYYSSVSNEYLVDVGGKNLVQGSEARHLVLVPQEGNGGLFDVQVENNLFST
                     ADKPEVAVYTVLRKAAEGIHTMEVEHSATETRWAAGAVSGVGCFASATESLGLDGGFT
                     LQVGTDGMVASSGSVPGGVVLDAPGPGDALIHLLRIGAGGAEKTIEMTWDGVSSVWML
                     DGTVDAGEELTLSELASYINSAWSGPVTARVDGGRIFLESPDGYLVSVTDARGRVSDA
                     LGMTRSSPAVSIEVNSTDSLQTIANRINSAYGSEGGPARPEEWLHATVEQTHGGLWYL
                     KLESNLVGEAYRINVGPEDGISLYTARNLGLVDPQGATRYSAIARDALFTV"
     gene            1810..2508
                     /gene="flgA"
                     /locus_tag="P5541_05680"
     CDS             1810..2508
                     /gene="flgA"
                     /locus_tag="P5541_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRV71507.1"
                     /translation="MKKSLFFAALLALLAFSAPAISMEPVSLEGLQVRWGEGTETLSG
                     MLRQRGGFPWSGITMEPGSFPFSGEIVSPKRIYPGTSSAVFRIRTWGGGTESFVARLT
                     WHAPMVLALEEISRGSVITGSNAAVQVAPYRRGYGKVFCSLPQVEGKRASRDIRAGEP
                     LSGRNIEVILLVERRDPVVVISKSGAVIARLRGVALEGGDLGDVITVRVPRYRNEIPA
                     TVIDRGLVEAAAGL"
     gene            2530..3105
                     /locus_tag="P5541_05685"
     CDS             2530..3105
                     /locus_tag="P5541_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRV71508.1"
                     /translation="MRIVKILFLMVAATLVFSAPAIAESLWAEGSSLFQDHRPDKVGD
                     IVTVVVSEKTTAKDSAKTDLTKKNDTTLADGFGLLDFIKKLGFSSSSEMSGGGTRDRS
                     HSFNATITCIVTEVLPNGNIVLEGTKEIRVQKENMSMKITGIARPQDVKAGNILESDR
                     LADVSVNVRGIGSISDVQQPGFLTRLLNIIF"
     gene            3115..4236
                     /locus_tag="P5541_05690"
     CDS             3115..4236
                     /locus_tag="P5541_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRV71509.1"
                     /translation="MKQLKKIFIAMLISLSALLAAGILPAGGQVHPEVRLKDLGRFEG
                     VRPNQLVGMGLVTGLQGTGDKGELVAAMMGNMVENFGISMNRKDLKSKNSAVVTITCE
                     LPPFARSGETIDVTISTVGDAKSLEGGVLLQTPLKAANGSVYAVAQGPVSIGGFSASA
                     AGSSLSKNVSTTGRIPNGAIVERETSWGAGTDGRIRFFLKRPDFTTADRVAAIINRNY
                     GSVATVTDAGCVDIAIPHAFRGNPSGFIASSELLRVRPDSVARVVINERTGTVTVGGD
                     VRISEVAVAHGNLTVSVGIDRTVSQPEPLSLGETKVIETATITADEERGSMALLNATT
                     TVDDVVKSLNALGATPRDIITILQAIDRAGALQGELVIM"
     gene            4249..4563
                     /locus_tag="P5541_05695"
     CDS             4249..4563
                     /locus_tag="P5541_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71510.1"
                     /translation="MPEKILPVVPAGTSSNRKDNARIEKACRDFEAIMVAALMKETSA
                     GKASRGTDGERNTFGALEETAWEMTAGAVSSRGEGFGLWRQLYESITKGTSGGEGHES
                     DK"
     gene            4547..5026
                     /locus_tag="P5541_05700"
     CDS             4547..5026
                     /locus_tag="P5541_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71511.1"
                     /translation="MNPTNSTNDIAALVDILVRSSRNQERTLGDLLEILQRESEALRQ
                     GRAELIPEILGELQDGSSRAMRCEAERDSSARKLAEVLGCPPAAGEICSRLDPSLCGE
                     LLEASRGLMCAVSSLSDINRLISRQADEHGRLAELVVENMRAMTPATDSSGGLDTTA"
     gene            complement(5047..5586)
                     /locus_tag="P5541_05705"
     CDS             complement(5047..5586)
                     /locus_tag="P5541_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRV71512.1"
                     /translation="MDGVAQKVPERDYSHNVGMGDTLLARVPGILSSLGLGSCLAVAL
                     FDDRAGIAGMVHIMLPREFRSPRGDLPDKPANPKPGKFADRALRNLVDLLEKEGAARS
                     RLRAKVAGGAKMFSISRGPNLLSIGEQNIEVVLEELKILKIPLEGKDTGGNKGRSVTF
                     FTETWEMEVRAIGQTPFRI"
     gene            complement(5586..6209)
                     /locus_tag="P5541_05710"
     CDS             complement(5586..6209)
                     /locus_tag="P5541_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRV71513.1"
                     /translation="MDQEGMNLVQMDLLKEIANIGTGHSATALSQMLGRGVDMFVPDV
                     EQIPLEEISSFFGDPELPVCAVLTRCDENLPLNLMLIMPESDSRALSEIMLSSITLPS
                     DLAMAEALRESALSEAGNIILGAFISAVGDFMKVELPLSVPAVAHDMLGAILDVIISI
                     FGVTGDTAFLADTRMTIPEEGNRVFSARVFLVPDPDSLGEFMRLLGF"
     gene            complement(6232..7299)
                     /locus_tag="P5541_05715"
     CDS             complement(6232..7299)
                     /locus_tag="P5541_05715"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRV71514.1"
                     /translation="MIPGGRIRVLVTDDSAFMRIVLGDFISAAPDMEVVGKARNGPDA
                     LEKIARLRPHVVTMDVEMPGMDGLQTLRAIMETSPLPVIMISGRTGRGTETTIMALES
                     GAFDFIRKPSGQDPEELAQLAQELQDKIRLAFASVAVLPGMEETPQERVPRSAATLPS
                     GGFGLLVIAASTGGPQTLAKLMPGLPSTFPAPVAIVQHMPRDFTRSFAERLDQISGLS
                     VMEAQNGMPLLPGMAVVAQGGLHLELVGEKGDIRCVLLDSPPKNYVRPSADILFSSAA
                     SIPGIIPAGIILTGMGKDGTEGAKELRDKGGFVIAESRETAVVYGMPRMAAEAGAVDR
                     VLPLHLMAGSLAEMFPWGDKK"
     gene            complement(7296..8135)
                     /locus_tag="P5541_05720"
     CDS             complement(7296..8135)
                     /locus_tag="P5541_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71515.1"
                     /translation="MDKKVVFVALAIRLGLESEGIPFISADAEPDRGGLDLVKVIFPS
                     RQGLADLDGFLKGNFIVRESEVNERPLRARMDPPPARYLVFPGPERMVLPEWKSFADA
                     PVTVEAITLFMDALDRSLGLLPLPPDRKFLDEMVSMAGALEERRLDIRPAEDLGSQCE
                     EAEGRLSSDDLALFFRTSPGITGRWFSAAAESGVNIQGAVTPLGQESDTPGFTALLKY
                     LRRRKVADIHGLSTLTEERLGSWDLLYQALEISRVRGIMTSASPTVFEFAHALFLASE
                     AHE"
     gene            complement(8253..9995)
                     /locus_tag="P5541_05725"
     CDS             complement(8253..9995)
                     /locus_tag="P5541_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV71516.1"
                     /translation="MDWFWNLKTKHKLLAGFLSVALIIAVVGFVGLFNMYRINEGVKT
                     MYHDRLVPIQDLGEIEKNIVAIRADFWQAMSVSDLDIVQEAFNFFDLHFEAVNKALYQ
                     YNQSYMEEDEKALVEELMVTVREYEKSGNAFKKMIIFGETHDKLLKFLVEEIRPKRLT
                     TEDIARELLALNREIADGISVSAEKAYSVSLALIIGLTAVAVIFAIGAGMFIGKVIAH
                     PLIRLSGVLDGFSRYDLTLEEDNPLEEYRYWQDEIGIIAGGISALQKSFVDILKGIDS
                     QSNQVASSSEEMMATSEQTATAAEEIAKTIEEMAEGASDQARDTELGSRRVAELGDLI
                     EQDVHFMKELNLSADEVASLKEEGAAVLGDLLEKTRESNDAAKLIFGIISETNDSAEG
                     IKAASGVIKSIADQTNLLALNAAIEAARAGEHGRGFAVVAEEVRKLAEQSNDSVQEIE
                     GIINELASKTHKAVSTMEEVRGIVSAQTECVNGSMEKFEGIAGAIEKTRGVIEKLNVS
                     GREMEGKKAEIIEVIQNLSAIAQENAAGSEEAAASIEEQTAAVQELSGASQELAKLAE
                     EMQAAIGQFRFQTS"
     gene            complement(10143..10457)
                     /locus_tag="P5541_05730"
     CDS             complement(10143..10457)
                     /locus_tag="P5541_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71517.1"
                     /translation="MGDSDKVTRKPPVEPLKVQNTLYDRRGLAFHAAPAAGTESLEQA
                     VRLLEKVCGCFGREIFGSINGDEVLFRDRKGTVAAIGAARALSIASELRVILGALLDR
                     EA"
     gene            10588..11646
                     /locus_tag="P5541_05735"
     CDS             10588..11646
                     /locus_tag="P5541_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV71518.1"
                     /translation="MGRKKHGGVCFLSTGSRFPVKNAQETEGATLEIVPKHDGQEEPG
                     PGDISREMPEIFSEDDIDAVQTLYDIFHGDIRDIPLLTAEEEKELSRRIWEEDDKEAF
                     DRFVLSNLRLVIACAKKVRDRMGDQSILSFMDLIQEGVMGLMTAVEKFDYRRNTRFST
                     YGIPWIYQRIKMSLLQHRYGMSIPGFASTSVHSMSRHIQAYRKGKIGDIPDNIDVGRI
                     RDLANIVSSMISISTDQDDDRTGGGYSLNPEKVCNDHFWDPWDLPGGTRCDEEVENGI
                     FCENITAILESTLTEEEYGILCRRFGIGEFNSPRRLSDIAKEYGKSAEHVRIVLNRSI
                     KRLRENGALKNACNSWGI"
     gene            11621..12136
                     /locus_tag="P5541_05740"
     CDS             11621..12136
                     /locus_tag="P5541_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV71519.1"
                     /translation="MPAIHGEYDLFAGDRQQEGVVDLDSIVVFRLGSQKYGVPIGSVK
                     EIIPCSDITPAPGTPPWFLGFMNVRGDLVPVVSLTRYIGMEYQTCNGMERILILMSER
                     GTRRGFQADEVTSIEPFSPDDIQSVRDRGNGSGFPRELIKGVSDTGKELVVVLDVERL
                     LAGEGVEDETA"
     gene            complement(12147..12608)
                     /locus_tag="P5541_05745"
     CDS             complement(12147..12608)
                     /locus_tag="P5541_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71520.1"
                     /translation="MPSRSSSPEIQDLAFVDSSGRYIDGADFSFVNLRFPRLEKILQE
                     MILGGERNRRLVFQTGEVRPLNIEMLDPKGNFWKIWQEKETGNGVEEDPAIRFLFDGS
                     EGIIRPADYRDRLLRLEGNRKTPQLLISSFLMHWLSGLLALHSEVNLADLA"
     gene            12752..13273
                     /locus_tag="P5541_05750"
     CDS             12752..13273
                     /locus_tag="P5541_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71521.1"
                     /translation="MGRSTSEKSCANPADGTDNIQSIPERKVKSPAGRKQGYRMQAWQ
                     KELQLTSIRNRDLENFNSFLRDFAGVLQSFRGKEFFILSDTPQRNHLVPHERVFLLRD
                     VLKRDGGLSCNTLFLYQNVSLEWERVCLKVFDNYTLVREKKLFPHEEEAKTEMYRLIG
                     RLARTHEQDFPPY"
     gene            13400..13966
                     /locus_tag="P5541_05755"
     CDS             13400..13966
                     /locus_tag="P5541_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV71522.1"
                     /translation="MQESGKVRMAPVVSGEYVRKLRISLGLTQIQFAKIMEVGNITVA
                     NWEKNGIDGTRSSSFPNFKYLTTMLRQSMNHPGVVEPSRFVRYLRMAADHELMACYIP
                     FIGELETDYLNVINSGSLTGVLFALLFDKELQRRGIQEPSGIIPGEDTLSDSSFNEDP
                     ELLEGLEKVKKKSAPEGSGGRPKKPEKH"
     gene            complement(13983..14531)
                     /locus_tag="P5541_05760"
     CDS             complement(13983..14531)
                     /locus_tag="P5541_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71523.1"
                     /translation="MEGFFFRSGKYLRIPILDPGNVSYVSRIELIVSLISGLVEKWES
                     FYDVETSEVPQEMEQISYSIRRLAESLSSEEITHLEKQDFMVYARDIAGNFSSGGTDR
                     KKMQRAMRVICEGLGHVQSSLDYGYPAFVDPSPECMEPLIELVPVPSSAHGGDCSQKT
                     LQFGVEEEWDFSPLSILCAREG"
     gene            complement(14802..16445)
                     /locus_tag="P5541_05765"
     CDS             complement(14802..16445)
                     /locus_tag="P5541_05765"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HRV71524.1"
                     /translation="MARKDLTVSDILNIPVLKDCKLLAGSHGTAKRIVSRVSVMDSPD
                     VANWMWGGEFLLTTGYVVRDNPLSLKFIIEELCRRNVAALGIKLGRFLTSLPDEILAA
                     AEEMDFPLLCIPGHLAFSDILGPVVCQINTRQNESSEKQTSRLLFQDHVSRSFLELML
                     AGGGVKAILHHLGALINVDVAFFDRFSGAWASPEEKSHFVENISSISSRSMRETFHSF
                     PLDLDEVNYGHIIIDHPREAPFPESWKWTLDYAKIAILLNIQKENARKEARLRYKDNF
                     LQGLVLGHINRQEDIHEYLFVLGPEFLPPFVVILGDTDTEGGPSKVTGISGSVDDPVS
                     LRDEIWSSLNNFLGAYFGRIHSTMVGERHVSLVSLHPDPGEKIHLLEMLLGQFKENFS
                     SERGKTFSIVVGGSVRDIGRISSSFRQASCCMEFVRATGSTDALLVWEKMGLLRLLMN
                     TSNREEIKIYVDQYLGAFGFLKPSVAREYLETLYVLCRNGWNLRSSAAEMHLHYNTLR
                     YRIRQISEIVSFDIEDPETRQELLIALKLYFANKEMHIF"
     gene            complement(16670..17137)
                     /gene="fliS"
                     /locus_tag="P5541_05770"
     CDS             complement(16670..17137)
                     /gene="fliS"
                     /locus_tag="P5541_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRV71525.1"
                     /translation="MLSRDRDIRQQYLNQEVIAASREKLLFLTYDIAIRACRNGASAI
                     ESGEIERANSEIQTAEDSIRELQFALRRERAEEVAESLNRIYDFMHDELVQANVAKDP
                     EKVRVVASMLEDLASTWEEALGKILEEGSLTRDEEKMLRSGVGISAGGLDVSL"
     gene            complement(17162..18910)
                     /locus_tag="P5541_05775"
     CDS             complement(17162..18910)
                     /locus_tag="P5541_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRV71526.1"
                     /translation="MDVSKTGMPLPGQIPAGEKPQKVDHGSGQASDGVNFESILSGEI
                     HAGPCDALGKGKCVSPSSGPVAGHTPERDLVTPAISAPEIEEIFRPAEAEMSRMGRRE
                     TIPAIDGETMAILEKCFGEMLVEREVPGIPDFEPAEGVSAVDESPGRRLLEGFVRLKM
                     DIDGFRPGGPEDPPETGPDEEGKSGDFATRAHEAGVLVFGDIPQRSISNLDGETSAPS
                     IASEPISFATKTKETSAPSIASEPISFETKTKETSAPSIASEPISFETKIGETSAPSI
                     ARIFHETPPGSKPEPGYADKKVEMTEVRKVSMKTEQSGISLQDVELKTAARNAYLGTR
                     INTPAENFEEGFPRGNVSRPVLPSGVGSTQVVDKTTATLTKTGVDPVPGNSEKTPGVL
                     KGLEGFREMDSFEARHLTRAKEDDPLRSEAVEFAVPVAGSRDPLGQVERFSPAVVGES
                     LARFVEVIRDRKGHRGTLELDPPELGRLRITLESTRDSLNLHLVVEKAHSRSMIEESL
                     GALRDSLARQGISLGETTVDVGGHAPQGRGSSPWVPGEQGGIPLTGAADAEDVTEQEQ
                     VVARLDIERGLFHWIA"
     gene            complement(19007..>20030)
                     /gene="fliD"
                     /locus_tag="P5541_05780"
     CDS             complement(19007..>20030)
                     /gene="fliD"
                     /locus_tag="P5541_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRV71527.1"
                     /translation="AGNSLSDVADAINEAAATSAGEGFAPAMTARILDNTLVISSSVT
                     GADFGLTVFGDDDNVLQGLGVWNVGGSGEFSRILQEPVDAVFEMDGLEITRSSNSIDD
                     LLEGVTFEIASEGSARVDISLDAGSAVESVRTMTEAYNEVMDWVNVRLSEKKVDEPQS
                     DLERRRGLLNGDPLLWNCKNNLRGITGQFRPLSNGESRLLSFAGISTESVDYGKSGKL
                     QFDETAFMEAMLEDPGSVMEVIQSFAAEMKTFTEGMISRSTISIGGTTAKKGRIYSQI
                     DTLERQSLDIDRRVANFEARLEMERASLEALYSNMESRIAMISKQASYITSLSQLGNY
                     GSGSDS"
BASE COUNT         4525 a         5659 c         5388 g         4458 t
ORIGIN      
        1 acggtgaaaa gggcatccct ggcaatggct gagtaccgcg ttgccccttg gggatctacg
       61 aggccgaggt tgcgggccgt gtagaggctg atgccgtcct cagggcccac gtttatcctg
      121 taggcctcgc cgaccaggtt gctctcgagt ttcaggtacc agagaccgcc atgggtctgt
      181 tccaccgtgg catgcagcca ttcctccggt ctggccgggc caccttcgga accgtaggcg
      241 ctgtttatcc tgttggcgat ggtctgaagc gagtcggtac tgttaacctc tatggacacg
      301 gctggggacg atctggtcat ccccagcgcg tcggagactc tccccctggc atcggtaacg
      361 gataccaggt agccgtcggg gctctcaagg aatattctcc caccgtcgac cctggcggtg
      421 acaggaccgc tccaggcgct gttgatgtag cttgcgagtt cggaaagggt cagttcctcc
      481 cccgcgtcga cggtaccgtc cagcatccag accgacgaga cgccgtccca ggtcatctct
      541 atggtttttt ccgcaccccc ggcacctatc ctcagaagat gaatcagagc atcgccaggg
      601 cccggggcgt ccagaacaac ccctccgggg acactgccgg aagaggccac catcccgtcg
      661 gtaccgacct gaagggtgaa cccaccgtca aggccgaggc tctccgtggc ggaggcaaag
      721 caccccaccc cggaaacggc tcccgctgcc caccgggtct ctgtggccga gtgttccacc
      781 tccatggtgt ggatcccctc cgcggccttt ctgagaacgg tataaacggc aacctccggt
      841 ttgtcggcag tggagaagag gttgttctcc acctggacgt cgaacaaacc gccgttcccc
      901 tcctggggga cgaggaccag gtgcctggcc tcgcttccct ggacaaggtt cttacccccc
      961 acgtcgacga gatattcgtt gctgacggag ctgtagtaga ccgatacgtc cacgagacgg
     1021 gccagttgct ccacgaggag gtctctcctg tccatgaggt cgttggggct gttccccata
     1081 gcctgtaccc tggatatctg tccgctcagg gccgcgatcc tgtcgatgag ggagttggcc
     1141 tcccctaccc tcaattctat ctcggtgttc agggagtccc ggtactcgga gaagttgccg
     1201 ctgatctgcc ccatcaggct ggagagattt tcggcctccc tcaggagaac ctcgcgggcg
     1261 gggccggaat cgggcctttt ctggaactcg tggagggcct ggacgaagtt gtccagcgag
     1321 gcggaaagtc ccgtcccgga gggctcgcag acgaacatct cgaggtattc cattgtcctg
     1381 gacagggcct cccagtaacc gccctctatc gactcctgcc ggaactgggc atcgaggaac
     1441 gagtccctta tccgggatat tgcgctgacc tcgacaccgg tgccaacctg cccgggggag
     1501 ccgggggaat tgaagcccac tgccgcgtag gggtcggccg gagccgcctc caccctctgg
     1561 cgggagtatc cctcgacatc ggcgttgctt atgttgtgac cggccgtttc tatcccctgc
     1621 cggaagtaat ccagcgccct cctgccgatc tcgagccccg aaaaggtacc gctcatgaat
     1681 gatcccccct ctttttggtt atcggcctgg aaaggggcat agaacagatt tcatcttttc
     1741 gcacccacgt ccgatactca atgtgagagg ttttgtgcag atcggcagcc aggaaggggg
     1801 aatggcaatt tgaagaaaag cctgtttttt gcggcgcttc tcgcgctgct tgccttctcc
     1861 gccccggcaa tatccatgga gcccgtctcg ctggaggggc tccaggtccg ctggggtgaa
     1921 gggaccgaga ccctttccgg gatgctcagg caaagaggtg gctttccatg gagcggcata
     1981 accatggaac cggggagttt ccccttctcc ggagagatcg tcagtcccaa aagaatatat
     2041 ccgggcacct cttccgcagt attcaggatc cgtacgtggg gaggcggaac ggagagtttc
     2101 gtggcaaggc tcacctggca tgcccccatg gtcctggcac tggaagagat ctcccggggc
     2161 agcgtcatca cgggctcgaa cgcggccgtc caggtggccc cgtacaggag aggttacggg
     2221 aaagttttct gcagcctgcc tcaggttgaa ggcaagaggg catcccggga cattcgggcc
     2281 ggggaacccc tttccggaag gaacatcgag gtgatcctcc ttgtggaacg gcgggacccg
     2341 gtggtggtca tctccaaaag cggggccgtc atcgccaggc tcaggggagt ggccctcgag
     2401 gggggcgacc tgggtgatgt cataaccgtc agggtcccca ggtacaggaa cgagattccg
     2461 gccactgtca tcgacagggg cctggttgag gccgccgctg gtctttagcg gcgtatgggg
     2521 gtgttagaca tgaggatcgt aaagatcctg tttctgatgg ttgctgccac tcttgttttt
     2581 tcggcgcccg ccatcgcgga gtcgctctgg gccgaaggct cttcgctttt ccaggaccac
     2641 aggcccgaca aggtgggtga tatcgtcacg gtggtcgtgt cggagaagac caccgcaaag
     2701 gattccgcaa agacggacct gacgaaaaag aacgacacga cccttgccga cggtttcggt
     2761 ttgctggatt tcatcaagaa actgggcttt tcctcctcaa gcgagatgag cggaggcggc
     2821 accagggaca ggagccacag tttcaacgcg accatcacct gcatcgtaac agaggtgctt
     2881 ccgaacggga acatagttct ggaggggaca aaggagatac gggttcaaaa ggagaatatg
     2941 tcaatgaaga taacggggat cgcgaggccc caggacgtga aggccggcaa tatccttgaa
     3001 agcgacaggc ttgccgatgt gagcgtcaac gtcaggggca taggaagcat aagcgatgta
     3061 cagcagccag gcttcctcac acggctcctg aacattatct tttaggtgaa aatcatgaag
     3121 cagttgaaaa agatctttat cgcgatgctg atttcgttat cggcccttct tgcggccggg
     3181 atccttccag ccggaggcca ggtgcatccc gaggtcaggc tgaaggatct tggaaggttc
     3241 gagggtgtcc ggccaaacca gcttgttggt atgggcctgg ttacaggact ccagggaacg
     3301 ggagacaagg gggaactggt ggcggcaatg atgggcaata tggtggaaaa cttcggaata
     3361 tccatgaacc ggaaggatct caagagcaag aacagcgccg tcgtaacgat cacctgcgaa
     3421 ctccccccct tcgcaaggag cggtgagacc atcgacgtga cgatcagcac cgtgggtgac
     3481 gccaagagcc ttgagggtgg cgtcctgctc cagactccgc tgaaggcggc caacggatcg
     3541 gtctacgctg tagcccaggg acccgtttcg ataggagggt tctccgcatc ggcagcaggc
     3601 tccagccttt cgaaaaacgt ctccaccacg gggcggatcc ccaacggggc catagtcgag
     3661 agggaaacct cctggggggc cggtacggac ggcaggatac gatttttcct gaagaggccc
     3721 gatttcacaa ccgccgacag ggtggcggcg atcatcaacc gcaattacgg ttccgtagcc
     3781 acagtaaccg atgcgggatg cgtggatatt gccatccccc acgcttttcg gggaaacccc
     3841 tcaggcttca tcgcctcttc ggaacttctt cgggtcaggc ccgattccgt cgccagggtg
     3901 gtaatcaacg agagaacggg gacggtaacc gtgggaggag atgtgaggat cagcgaggtg
     3961 gccgtagccc acgggaacct gacggtttcc gtcgggatcg acaggaccgt cagtcagccg
     4021 gaacctcttt ccctggggga gacaaaggtc atagagaccg cgacgataac cgccgatgag
     4081 gagaggggaa gcatggccct ccttaatgcc accaccaccg tggacgacgt ggtgaagagc
     4141 ctcaacgcac tgggggcgac cccgagagat atcataacga tcctccaggc catcgacagg
     4201 gcgggggccc tgcagggcga actggtaatc atgtagggag gggcaaagat gccagaaaaa
     4261 atcctgcccg tggtaccggc gggcacctcg tcgaacagga aggacaacgc aaggatagag
     4321 aaggcctgca gggatttcga ggccatcatg gtggccgctc tgatgaagga gacctccgcc
     4381 gggaaagcct cccgtggaac tgacggcgaa agaaacactt tcggcgccct ggaggagacg
     4441 gcatgggaaa tgacagcagg ggcagtatca tccaggggcg aagggttcgg gctctggaga
     4501 caactttacg aaagcatcac gaaaggaacc tcgggaggtg aaggacatga atccgacaaa
     4561 tagtacaaac gacatcgccg ccctggtgga catattggtg cgcagcagcc gcaaccagga
     4621 gagaaccctc ggggatcttc tggagatact tcagagggag agcgaggctc tgaggcaagg
     4681 aagggcagaa ctgatcccgg agatcctggg cgaactccag gatggctcca gcagggcaat
     4741 gagatgtgag gccgagaggg attccagcgc cagaaagctt gccgaggtcc tggggtgccc
     4801 tcccgcagca ggcgagatct gcagcaggct tgatccttct ctttgcgggg aactcctgga
     4861 ggcatcaagg ggtctgatgt gcgccgtctc ttctctgagt gacatcaacc ggctgatatc
     4921 gagacaggcc gacgaacacg gccgcctggc ggaactcgtg gtcgagaaca tgagagccat
     4981 gactccggcg acagattctt cggggggtct cgacacgaca gcctgaaaaa gggaataaac
     5041 ttcggactag atgcggaagg gggtctggcc tatggccctg acctccattt cccatgtttc
     5101 ggtgaaaaat gtgacgctcc ttcctttgtt ccccccggta tcctttccct ccaggggtat
     5161 cttcaggatc ttgagttcct ccaggacaac ctctatgttc tgttccccta tagaaagcag
     5221 gttgggaccc ctggatatgc tgaacatttt ggctcctccg gcaacctttg ccctgaggcg
     5281 agacctcgca gcgccttctt tctccaaaag atccacaagg tttctgagcg ccctgtcagc
     5341 aaacttcccc ggcttggggt ttgcgggctt atcggggaga tcacccctcg gggatctgaa
     5401 ttccctgggc aacataatat gcaccatgcc cgcgatgcct gccctgtcgt caaaaagagc
     5461 caccgcaagg caggaaccaa gcccaaggga gctcagaatt ccaggcaccc ttgcgagaag
     5521 ggtatctccc atccccacgt tgtgagagta atccctctcc gggacctttt gcgcaacgcc
     5581 gtccatcaga aacccagaag cctcatgaat tccccgagcg agtcgggatc gggcaccagg
     5641 aagaccctgg cggaaaagac ccggttcccc tcctcgggta tggtcatcct ggtgtcggca
     5701 agaaatgccg tgtcccccgt cacgccaaag atggagatga tcacgtccag tatggccccg
     5761 agcatgtcat gggccacggc ggggacagaa agggggagtt cgaccttcat gaaatccccc
     5821 acggcgctga tgaaggctcc caatatgata ttgcccgcct cggagagggc cgattcccta
     5881 agggcctccg ccatggccag gtccgagggc agggttatgc tggagagcat tatctccgag
     5941 agagccctgg aatccgactc gggcattata agcatcaggt tgaggggcag gttctcatcg
     6001 cagcgggtca gcacggcaca gaccggcaac tccggatccc cgaagaagga ggatatctcc
     6061 tccagcggta tctgctcgac atcagggacg aacatgtcga ctccgcgccc cagcatctgt
     6121 gaaagggccg ttgcagaatg tccggtccca atgttggcta tctccttcag gaggtccatc
     6181 tgtaccaggt tcataccctc ttgatccacg ccgacggccc ctcccttccg gtcatttttt
     6241 atccccccag ggaaacattt ccgcaaggct ccccgccata aggtgcaggg gcaaaaccct
     6301 gtccacagcc ccggcttcgg ccgccatgcg gggcattccg taaaccacgg cggtctcccg
     6361 ggattctgct atcacgaaac cgcccttatc cctgagttct ttcgcccctt cggtgccgtc
     6421 cttccccatg cccgtaagaa tgatccccgc aggaatgata ccggggatac tggcggcact
     6481 ggaaaagaga atatccgccg atggccttac ataattcttt ggaggagaat caagaagaac
     6541 acatcttatg tccccttttt ccccaaccag ttccaggtgc aaaccgccct gcgccaccac
     6601 ggccatcccc gggagaaggg gcatgccgtt ttgggcctcc ataacgctaa gtcctgatat
     6661 ctggtcgagc ctttcggcaa aggaccttgt gaaatccctc ggcatgtgct gcacgatcgc
     6721 cacgggcgcg ggaaaggtgg aaggaagtcc aggcataagc tttgccaggg tctgaggtcc
     6781 tcccgtggat gccgcaatga cgagcaggcc aaaccctccg gagggcagcg tggcggcaga
     6841 tcgcgggacc ctctcctgag gggtttcctc catcccgggc aggacagcga cggacgcgaa
     6901 ggccagcctt atcttgtcct gcagttcctg cgccaactgg gcaagttcct ctggatcctg
     6961 accagaaggt tttcgtatga aatcaaaggc acccgattcc agcgccatga tcgtggtttc
     7021 ggtcccccta cctgtccggc cgctgatcat gatgaccggg agggggcttg tctccattat
     7081 cgccctgagg gtctgaagac cgtccatgcc cggcatctcc acgtccatgg tcaccacgtg
     7141 aggccttagc cgggctatct tttcgagggc atcggggccg ttcctggcct ttccgacaac
     7201 ctccatgtcg ggggcagccg atatgaaatc acctagaacg atcctcataa aggctgaatc
     7261 atcggtcaca agaaccctaa tcctgcctcc agggatcatt catgtgcctc cgaggcgagg
     7321 aaaagagcat gggcaaactc aaagaccgta ggcgaggcgg aggtcatgat gcccctgact
     7381 cttgatatct ccagcgcctg ataaagaaga tcccagctcc cgagcctttc ttctgtaagg
     7441 gtcgagagcc cgtgaatgtc cgccactttc ctccgtcgaa gatatttcag gagggcggta
     7501 aatccgggag tatccgattc ctgtcccaaa ggcgtaacag caccctgtat attgaccccg
     7561 ctctccgcgg cggccgagaa ccagcgtcct gtgattcccg gactggtacg gaaaaacagg
     7621 gccagatcgt cactggacag tcttccctcg gcctcttcgc actgactgcc aagatcttcc
     7681 gcgggacgga tatccaaccg cctctcctca agagccccgg ccatggagac catctcgtcc
     7741 agaaatttcc tgtctggagg cagggggagc aggcctaggg atctatcaag agcgtccatg
     7801 aagagggtta ttgcctcgac agtgaccgga gcgtccgcga aggatttcca ctccggcagg
     7861 accatccttt ccggacctgg aaaaacaaga taccttgcgg gggggggatc catccgcgcc
     7921 ctgagcggcc tctcgttcac ttcactttcc ctgacaatga aattgccttt caggaacccg
     7981 tccagatccg caaggccctg gcgcgaaggg aaaatcactt tcaccaggtc caggcctccc
     8041 ctgtcgggtt ccgcatcagc ggatataaag ggaatcccct ccgattccag acccagcctg
     8101 atcgcaaggg ccacaaaaac cacttttttg tccatggggc tcacctcttt ttgacagtat
     8161 ccacccgcaa gagaagcggg cactgcgggt ttaatactat catcaagggc agaaccgcaa
     8221 aagcgagaac ccggggaccg cgggaaaccc ttttatgaag tctggaacct gaactgccct
     8281 attgctgcct gcatctcctc ggcaagtttc gcaagttcct ggctggctcc agacagttcc
     8341 tgaacagcag ccgtctgttc ctctatcgac gcggcggcct cctcgctgcc ggccgcattc
     8401 tcctgggcta tggccgaaag gttctggata acttcaatta tctcggcctt cttgccctcc
     8461 atctctcgtc ccgaaacgtt cagtttctct atcaccccac gggtcttctc tatcgctccc
     8521 gcgatgcctt cgaatttttc catcgaaccg ttgacgcact ccgtctgggc cgacactatc
     8581 ccccttactt cttccatggt cgatacggcc ttgtgagtct tggatgccag ttcgtttatt
     8641 attccctcta tctcctggac ggagtcgttg gactgctccg ccagtttcct tacctcttcg
     8701 gcaaccacgg cgaatccacg gccgtgctca cccgcacggg ccgcctctat cgccgcgttc
     8761 agcgcaagaa ggttggtctg gtccgctatg ctctttatta caccgcttgc cgcctttatt
     8821 ccttccgcgc tgtcgttggt ctcgctgatt atgccgaata tgagtttcgc ggcatcgttg
     8881 ctctccctgg tcttttccag gagatctccc agaacagcgg caccttcctc cttgagagac
     8941 gccacctcgt cggcgctcag gttgagttcc ttcatgaagt gaacatcctg ctcaatcagg
     9001 tcaccaagtt ccgcgaccct tctggacccc aattcagtgt ccctggcctg gtcactcgca
     9061 ccctcggcca tctcctctat agtcttcgct atctcctccg ccgccgtcgc agtctgttcg
     9121 ctcgtcgcca tcatctcttc cgaggacgac gccacctggt tcgactggga gtcgataccc
     9181 ttgaggatgt caacgaaact cttctgcagg gccgatatcc cccccgcaat gatccctatc
     9241 tcgtcctgcc agtacctgta ctcctcaaga gggttgtctt cttccagcgt caggtcgtag
     9301 cgggaaaatc cgtccagcac acccgagagc cggatcaaag gatgggcaat gaccttgccg
     9361 atgaacatcc ctgccccaat ggcaaatatc acggccacag ccgtaagacc gattatcagc
     9421 gccagagata ccgaataggc cttctcggcg gaaacactga tcccgtcggc aatctccctg
     9481 ttgagagcca gcagttccct cgcaatatcc tccgtcgtca aacgttttgg tcttatctct
     9541 tcaacgagga attttaaaag tttgtcatgc gtttcaccaa agatgatcat ctttttaaaa
     9601 gcattccccg atttttcata ctcccttacg gtaaccataa gttcttcgac cagggctttt
     9661 tcgtcttctt ccatatagct ctggttatac tgatacaggg ccttattgac ggcctcgaaa
     9721 tggagatcaa aaaaattgaa ggcttcctga actatgtcca gatcggatac ggacatggcc
     9781 tgccagaagt ccgcccggat cgccacgatg ttcttctcga tctcgcctaa atcctgtatt
     9841 ggaacaagcc tgtcatggta catggttttg acgccctcat tgatgcggta catgttgaaa
     9901 aggcccacaa agccgaccac cgctatgata agagcgacgg aaaggaaccc cgccagcaac
     9961 ttgtgtttcg tcttgagatt ccagaaccag tccatctgtt gcaacctccc ctctcccgga
    10021 tcgctcctcc aaaggctcta catctaccag tttaagagag ggacactgcg gaaacagaaa
    10081 ccatagccct tccggggtca ccccctggcc atggacagga tcggacagaa aggccccttg
    10141 atttacgcct ccctgtcgag aagggctccc agaataaccc gcagttcact ggctatggaa
    10201 agcgcccttg cggccccgat ggcagcgaca gtaccctttc tgtctctgaa aagaacctcg
    10261 tcgccgttta tgctgccgaa aatctcacga ccgaaacatc cgcaaacctt ttcgagcagg
    10321 cgcacagcct gctccagaga ttctgttccg gcagcgggcg ctgcatgaaa ggccaatccc
    10381 ctcctgtcgt agagagtgtt ttgcaccttg aggggttcta ccggcggttt cctggtgacc
    10441 ttgtcggaat cgcccatgat atccacctcg tcctgtaaat attgatccct ttgaagagta
    10501 taagaggaaa gcaggcaagt aacgctaatt aaggcggcct cctcttgccg atacctattt
    10561 aaaacgggtc cagggaggga gccgaatatg ggaaggaaaa agcacggagg cgtttgtttc
    10621 ctttctacgg ggagtcgttt ccccgtaaag aatgcgcagg aaacagaggg ggcaacactt
    10681 gagatcgtcc caaaacacga cgggcaagag gaacccggcc caggggatat atcccgggag
    10741 atgccggaga tcttttccga agatgacatt gatgcagttc agacactgta cgatattttc
    10801 cacggagaca tcagggatat tcctttgctg acggcggagg aagagaagga actctcgaga
    10861 agaatatggg aagaggatga caaggaggct tttgacaggt tcgtcctgtc aaatttgcgg
    10921 cttgtcatag cctgcgcaaa aaaggtgagg gaccggatgg gcgaccagag tatcctctcc
    10981 ttcatggacc tgatccagga gggagtaatg ggcctgatga cagccgttga aaaattcgac
    11041 taccggagga acacccgttt cagcacttac ggcattccct ggatatacca gagaataaag
    11101 atgtctctcc ttcagcacag gtacggaatg tcgatcccgg gtttcgcttc gacctctgtc
    11161 cattccatgt cgaggcacat ccaggcctac agaaaaggca agatcgggga tataccggat
    11221 aatatcgatg tgggcaggat ccgggacctg gcaaacatag taagttccat gatctccata
    11281 tcaacggacc aggatgacga cagaaccggc gggggatact cgctgaaccc ggaaaaggtg
    11341 tgtaacgacc atttctggga cccctgggac cttccgggag gaaccagatg cgatgaagag
    11401 gtcgaaaacg gcattttttg cgaaaatatt acggctatac ttgaaagcac gctgacagaa
    11461 gaggagtacg gcatcctctg ccgcaggttc ggcattgggg aattcaattc tccaaggagg
    11521 ctctccgaca tagcaaaaga atacggaaag agcgccgagc atgtgaggat cgtcctaaac
    11581 cgctcgataa aaaggctcag ggaaaacggg gccctgaaga atgcctgcaa ttcatgggga
    11641 atatgatctt tttgccggtg accggcaaca ggaaggggtt gttgatttgg acagcatagt
    11701 ggttttcagg ctggggtcac agaaatacgg ggtgccgata ggctccgtga aggaaataat
    11761 accctgctcc gatattacac cggccccggg aactccacca tggttcctgg gattcatgaa
    11821 cgtaagaggg gatcttgtcc ccgtggttag cctgacccgc tatattggca tggagtatca
    11881 gacatgcaac ggcatggaaa ggatactgat ccttatgtcc gaaaggggta ccagaagagg
    11941 gtttcaggcc gatgaggtaa catcgataga acccttctcg cctgatgata tacaaagcgt
    12001 cagggaccgt ggaaacgggt caggtttccc cagagagttg attaagggcg tttccgatac
    12061 cgggaaagaa ctggtcgtgg tcctggacgt ggagaggctt ctcgcgggag agggggttga
    12121 agatgaaact gcctgagacc acggactcat gcaaggtctg ccaggttaac ttcgctgtga
    12181 agagccagca accccgagag ccagtgcatc aggaaagatg aaatcagcag ttgaggagtc
    12241 ttgcggttcc cttcgagccg aagaagcctg tccctgtagt cagcggggcg gataattcct
    12301 tctgaaccgt cgaaaaggaa acggatcgcc gggtcctctt cgactccgtt gcctgtttct
    12361 ttttcttgcc agattttcca gaaattaccc tttggatcga gcatttctat gttgaggggt
    12421 cggacttcac ccgtctgaaa aaccagcctc ctgttccttt cacccccgag gatcatctcc
    12481 tgaagtattt tctccagccg gggaaatcgt aaatttacaa aggagaaatc ggcaccatca
    12541 atatacctcc cgctcgagtc gacaaaggca agatcctgta tctctgggct gctgcttctt
    12601 gaaggcactt cccctgcctc cctccggaag attattgaaa cggcttgttc catttgccgg
    12661 gaatatcgct aaaagcctta attagcgtca ccgcaaaggc cgatacctaa aacgaaggcg
    12721 caacgaacgc aacctccgga ggtgttctaa cttgggcaga tcgaccagcg aaaaatcctg
    12781 cgcaaatcct gcagacggga cagataacat ccaaagcatc cccgagagaa aggttaaaag
    12841 cccggccgga aggaaacaag ggtacaggat gcaggcatgg caaaaagaat tgcagttgac
    12901 atcgatcagg aacagagatc tggagaattt caacagtttt ctgagggatt tcgcaggggt
    12961 cctgcagagt ttcaggggga aggagttctt tattctgagt gacactcctc aaagaaacca
    13021 ccttgttccc catgagagag tcttcttgct gagggatgtt ctcaaaaggg atggaggtct
    13081 ctcctgcaac actcttttcc tttaccagaa cgtttccctc gagtgggagc gggtatgcct
    13141 gaaggtcttc gataattata cactggtccg ggaaaagaaa ctttttcccc acgaggaaga
    13201 ggcaaaaacc gagatgtacc gactgattgg ccgccttgcc agaacccacg aacaggactt
    13261 tcccccgtat tagggacaat tatggtgttt aagtatatgt ttatttgtgt gatgctttgt
    13321 attattatat agaggccatg ttcgtgtgga gacaccccgg tttcttcaag aaaatctgaa
    13381 tcatggagtg tgaaggatca tgcaagagag cggcaaggtt agaatggctc ctgtcgtttc
    13441 gggtgaatat gtcagaaaac tccgtataag cctgggactg acgcagatcc agttcgccaa
    13501 aataatggaa gtcgggaaca tcacggtggc caactgggag aagaacggaa tagacggtac
    13561 cagaagttcc agtttcccca acttcaagta cctgacgacg atgctcaggc aatcgatgaa
    13621 ccaccctggt gttgttgaac cctccaggtt cgtgagatat ttgcgcatgg cggcagatca
    13681 cgaacttatg gcctgttata tcccattcat cggggaactg gagacggatt acctcaacgt
    13741 tatcaactcg ggaagcctga cgggagtcct ttttgcgctc cttttcgaca aggagctcca
    13801 gaggagaggt atccaggagc cgtcagggat aattcccggg gaagataccc tttctgattc
    13861 atcgttcaac gaggatcctg agcttctcga gggccttgag aaggtcaaaa aaaagtctgc
    13921 ccccgaagga tccggtggca gaccaaaaaa gcctgaaaaa cactgaatgg gatgagtacc
    13981 ctttaaccct cccgggcgca aagaatggag agcggggaaa aatcccattc ttcctccacc
    14041 ccgaactgca gggttttctg ggaacaatcc ccgccatggg cggaagacgg taccggaacc
    14101 agttcgataa ggggctccat acattcggga gaagggtcaa caaaggcggg atagccataa
    14161 tccaggctcg actggacatg gccaagacct tcgcagataa cccgcattgc cctctgcatc
    14221 tttttccggt ctgttccccc actggaaaag tttccggcaa tgtccctggc atataccatg
    14281 aagtcctgtt tttcaaggtg ggtgatctct tcagaagaga gcgattcggc gagcctgcgg
    14341 atggaatagg atatctgctc catttcctgc ggaacttctg aagtttccac gtcataaaat
    14401 gattcccatt tttcgacaag gccggaaatc aaagatacaa tgagttcgat cctcgaaaca
    14461 taggaaacgt tgcccggatc aagaatcggg atcctcaggt atttgccaga ccggaaaaag
    14521 aacccttcca tttctgtatc cctcctcgct ttttcaggaa aattatgtgc agaagtacct
    14581 cgagccaggc gataccagaa taatccggcc accacagtga cggtattccg attttccgat
    14641 tggctctccg ggtaccacaa aaaaccctca ggaaggagta acagatagaa ggtagcacca
    14701 acgactgaac ttgtattcat cagaaaggaa caaaaaaaac cgtggtcgtt taaaaaacag
    14761 ttattaaaag gtttctgctc caggttttag aaacaaaatt gctaaaaaat atgcatttct
    14821 ttgtttgcaa agtacaattt taacgctatc aggagttcct gcctcgtttc gggatcctct
    14881 atatcgaagg atactatctc ggagatctgt cttatcctgt acctgagtgt gttgtagtga
    14941 agatgcattt cggccgcact cgacctgagg ttccatccgt tcctgcagag aacatagagg
    15001 gtttcgagat attccctggc aacagaaggc ttgaggaaac cgaaggcccc aaggtactgg
    15061 tctacataga tttttatctc ttccctgttg gaggtattca tcagaagcct cagaagcccc
    15121 atcttctccc agaccaagag ggcatcagtg gaaccggtgg ccctgacaaa ttccatacag
    15181 cacgaagcct gcctgaagct ggatgatatc cggccaatat ctctcactga acctcctacg
    15241 actatcgaaa aggtctttcc cctttctgaa gagaagttct ccttgaactg ccccagaagc
    15301 atctcgagaa gatgtatctt ctctcccggg tccgggtgga ggctgacaag agagacgtgc
    15361 ctttccccca ccatggtgga gtgtatcctg ccgaaatagg cgcccagaaa attgttgagg
    15421 cttgaccata tctcatcgcg gagagaaaca ggatcatcta ctgaaccgga aattcccgtc
    15481 acctttgagg gccccccctc ggtatcagtg tcgcccagga tgacgacgaa gggggggagg
    15541 aattccggac cgagaacgaa caggtactca tgaatgtcct cctgcctgtt tatatggccg
    15601 aggaccagcc cctgcaggaa gttgtccttg tacctgagcc ttgcctcttt tctggcgttc
    15661 tctttctgga tgttcaggag tatggctatc tttgcataat caagggtcca cttccagctt
    15721 tcgggaaaag gggcctctct ggggtgatcg ataatgatat gtccgtagtt cacctcgtcc
    15781 aggtccaggg ggaaagaatg aaaggtttcc cgcatactcc tcgaggaaat agatgaaatg
    15841 ttctccacaa aatggctttt ctcttcggga gaggcccagg cccccgaaaa gcggtcgaaa
    15901 aatgccacat ccacatttat cagggcccca agatggtgca ggatcgcttt aacccctccc
    15961 cccgcgagca ttagctcaag gaaggagcgg gagacgtgat cctgaaagag aaggcggctg
    16021 gtctgctttt cggaagattc gttctgtctt gtgttgatct ggcaaacaac aggtccaaga
    16081 atatcggaaa atgcaagatg gcccggtatg cagagaaggg gaaaatccat ctcttcagca
    16141 gcggccagga tctcatcggg aagggaggtc aggaagcgtc ccagttttat ccccagggca
    16201 gcgacgtttc gccggcagag ttcctcgatg atgaacttca gagagagagg gttatcccgg
    16261 accacgtaac cggtggtaag gagaaactcg cctccccaca tccagttggc aacgtcagga
    16321 gaatccatga cgctcacacg gctcactatt ctctttgccg tcccatggga gcctgcgagg
    16381 agtttgcagt ccttaagaac aggaatattc agaatatccg atacggtcag atcttttctg
    16441 gccattagcc ctctctcgtg aacaagactg gatgcttgcc gataagatta accttggtct
    16501 catttattgt caactattga cgttatccct gtcttctgcc aggaatattt tcccgtcttt
    16561 gctatctcta ccaaaacatt ccctcaaaat ttttgttaac aaataacaaa acgggagggg
    16621 gcccccctcc cgttgaacag cctcctgtat ttttttttaa cgatgggttt cagagggaaa
    16681 cgtcaagccc cccggcagaa atccctacac cggatctgag catcttctcc tcatcccggg
    16741 tgagggaacc ttcctcaagg atcttcccaa gggcttcctc ccaggtcgac gcgaggtcct
    16801 ccagcatgga tgcaacgacc ctgaccttct cgggatcttt cgctacattg gcctggacaa
    16861 gttcatcgtg catgaagtca tagatgcggt tcagactttc ggccacttct tctgcccttt
    16921 cgcggcgcag tgcgaactgg agttcccgta tggaatcctc cgctgtctgt atctccgagt
    16981 tggccctctc tatttcccct gactctatgg ccgaggcacc gtttctgcaa gctcttattg
    17041 cgatatcata ggtaaggaaa agcagttttt cacgcgatgc ggcgatcact tcctggttaa
    17101 gatactgctg gcggatgtcc cggtctctgg aaagcaatgc ttctatcccc tttctccggt
    17161 tttacgctat ccagtggaaa aggcctctct ctatatccag cctcgccacg acctgctcct
    17221 gttctgtcac atcttcggcg tccgcggccc cagtcaaggg aataccgccc tgttccccgg
    17281 gcacccaggg ggatgaacct ctgccctggg gcgcatgccc gccaacatcg acagtggtct
    17341 ctccgaggct gatcccctgc cttgccaggg agtccctgag agctcccagg gattcttcta
    17401 tcatacttct ggaatgagct ttctccacga ccagatgcaa gttgagactg tctctggtcg
    17461 attccagggt gatacgaagt ctgcccagtt ccgggggatc aagttccagt gtgcccctgt
    17521 gccccttcct gtcccttata acctctacaa accgggcaag gctttcaccc acaacggccg
    17581 gggagaacct ttcaacctga cccagaggat cacgggaacc ggcaacgggg acagcaaact
    17641 caactgcttc agaacgcagg gggtcatctt cctttgcccg ggtcaaatgt cgtgcctcga
    17701 aagagtccat ttccctgaag ccttcgagcc ccttcaatac tccgggagtt ttttcggagt
    17761 tgccgggtac agggtctacc cccgttttcg tcaaggttgc agttgttttg tcaaccacct
    17821 gagttgatcc aacacccgag gggaggaccg gtcgggaaac attgccacgg ggaaacccct
    17881 cttcaaagtt ctccgcaggg gtattaatcc ttgtccccag gtatgcattt cttgctgcag
    17941 ttttcagctc cacatcctgc aaagagatgc cgctctgctc tgtcttcatc gaaacctttc
    18001 ggacttcagt catttcaact tttttgtctg catatccggg ttcgggcttc gatccgggag
    18061 gggtttcatg aaagatcctg gctattgatg gagcggatgt ctccccgatc tttgtctcaa
    18121 aggagatcgg ctcgctggcg attgacggag cggatgtctc tttggtcttt gtctcaaagg
    18181 agatcggctc gctggcgatt gacggagcgg atgtctcttt ggtctttgtc gcaaaggaga
    18241 tcggctcgct ggctattgac ggagcggatg tctctccatc aaggtttgaa atgcttcgct
    18301 gcgggatatc cccgaagacg aggacgcccg cctcatgggc gcgagtcgca aagtcaccgc
    18361 ttttcccttc ttcatccggt ccggtttcgg ggggatcttc gggtcccccg ggcctgaagc
    18421 catcgatatc catcttcaac ctgacaaaac cctcaagcag tctcctgccc gggctttcgt
    18481 caacggcgga cacaccttca gccggttcga aatccgggat accgggaacc tccctttcga
    18541 ccagcatctc cccgaaacat ttttcaagga tagccattgt ctcgccatct atcgcgggaa
    18601 tcgtttcccg gcggcccatc ctggacattt ccgcctctgc cggcctgaag atctcctcta
    18661 tctcgggagc gctgatcgcg ggggtaacca gatccctttc tggagtgtgt cccgctacag
    18721 gaccggagga gggggataca catttcccct tgcccagagc atcacacggg ccagcatgga
    18781 tctctccgga aaggatagac tcgaaattaa ccccgtcact tgcctgtcca cttccgtggt
    18841 caaccttctg aggcttttcc cccgccggga tctgaccggg aagaggcatt ccggttttgc
    18901 tgacgtccat gaccgacccc cctttccatg gttatattcc accgccatat cttaagtttc
    18961 ggccatcggg agcgggtaaa acagtccctt cctcccttgg ttcatttcaa ctgtcgctgc
    19021 cgctgccgta gttccccaac tgcgataatg aagttatgta tgacgcctgc ttgctgatca
    19081 tggctatcct tgactccatg ttcgaataga gggcctccag agaagctctc tccatttcaa
    19141 gacgagcctc gaaattcgcc acccttcggt cgatgtccag ggattgcctc tccagtgtgt
    19201 ctatctggct gtagatcctg cccttttttg ctgtggttcc tcctatgctt attgtcgatc
    19261 tcgaaatcat gccctcggta aaggtcttca tctccgcggc aaaggactga attacctcca
    19321 taaccgatcc cgggtcttca agcatggctt ccatgaaggc cgtttcgtca aactgcaatt
    19381 tcccgctttt tccgtaatca acagattccg tcgatatacc tgcaaaagag agcagccggg
    19441 attctccgtt cgaaaggggc cggaactgac ccgttatgcc gcgcaggttg tttttgcagt
    19501 tccatagcag gggatcaccg ttaagcagac ccctccttct ttcaagatcg gattgtggct
    19561 cgtccacctt tttttccgaa aggcggacat tgacccagtc catgacctcg ttataggctt
    19621 ccgtcatggt tcgcaccgat tcgacagccg acccagcgtc aagggaaata tccaccctcg
    19681 cggagccttc cgaggctatc tcgaaagtca caccttcaag caggtcgtcg atactgttcg
    19741 atgacctggt gatctcgaga ccgtccattt cgaagaccgc atcgacaggc tcctgcaaaa
    19801 ttctcgagaa ttctcccgaa ccgccgacgt tccagacacc caggccctgg agaacattgt
    19861 catcatcacc gaatacggtc agaccgaagt ctgcccctgt aacactgctg gagataacca
    19921 gggtattgtc cagtatcctt gctgtcatgg cgggagcgaa accttccccc gccgacgttg
    19981 ctgccgcttc gtttatcgcg tccgcaacgt cggaaaggct gtttcccgcc
//
LOCUS       DAOTIQ010000035        19447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3373_length_19447_cov_8.841842, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000035 DAOTIQ010000000
VERSION     DAOTIQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_19447_cov_8.841842"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1049
                     /locus_tag="P5541_05785"
     CDS             351..1049
                     /locus_tag="P5541_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV71528.1"
                     /translation="MTDRKLRHTRIYEQVVNELKELIASGELQPGDPLPPERQMMAQM
                     GVSRSSLREAFRVMELLGLIDSVPGKGRFVRKPRGETADSRNMPLEDEAVLELMEARR
                     VLDPAIAAAAAMHSSPSDLMKLRKVLSRTEKNIENVGKRSQDDFDFHLALAESTHNFV
                     FVNIVKMTFNLIMATHDRIYSLLADKEAFLKEHQEIYGAIIDHDVARARTLSASHIER
                     VYKTLQEALALEVK"
     gene            1099..1653
                     /locus_tag="P5541_05790"
     CDS             1099..1653
                     /locus_tag="P5541_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71529.1"
                     /translation="MRDRIIKMIPEIDWIQDKDLRTKVVATVEDALKTGGWEPEDMEK
                     IPFTLLIPDCPASLNVHNRGVTRMAKKIFEEYNSLYKDNGGFQLDHDTLIAGAILHDV
                     GKLVEYEKKDGKAVKSQMGKDLRHPFSGVGLAMRNGIPSGICHCIAVHAKEGDGGHRS
                     PEAVVINKCDFINFETIKSFLGLL"
     gene            1687..2967
                     /locus_tag="P5541_05795"
     CDS             1687..2967
                     /locus_tag="P5541_05795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HRV71530.1"
                     /translation="MGKTMIEKIMKRAAGREVNVGERVWCKIDLSTARDFGGANCVLQ
                     FEEVTDKKGKVWDPDKVAFTFDLQAPSHSEKVSTNQKIIRDFAKKQGIKKVFDINWGI
                     GQHVMLENGLVKPGDVILGTDSHMNLLGAVGAFATGVGNTDIVASWFEGTNWFRVPET
                     MKITVNGSFKKGVCMRDLLTSIVGTLHADGMFFLSVEFYGDTIEKSSLADRITLCSMV
                     TEMSGKIGLIMPNGKVLDWLVERAGKEVLERVSSLAADPDAVYCQELEFNVNDLEPMT
                     SCPDAPDNVKKVREVAGAHVDQVHIGSCSNGRFEDITAAFEVLKAANFRIAPGIRAII
                     TPSTREVMLQCAEAGYIQKFLEAGVVFTNPTCALCTAEHYGALPSGDVGMSTTNRNFI
                     GKVGKGSHTYLASPMTAMATAVRGVVTDPRDILG"
     gene            2982..3509
                     /gene="leuD"
                     /locus_tag="P5541_05800"
     CDS             2982..3509
                     /gene="leuD"
                     /locus_tag="P5541_05800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301535.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRV71531.1"
                     /translation="MDKTILRGKAWVFGDDIDTDLIYHNKYLAETDPKNMPQYSFEYL
                     PGKENFAKEVKEGDFVVAGTNFGCGSSREHAVYCLKYAGVPVILAESFSRIYYRNAIN
                     NGYPVLFVKGLSQAIKDGKIKDGDELEVNLLKGEIKDVTSGNTFHGDAVADLEKDIMK
                     AGGLIEYLKEKAAKK"
     gene            3529..4785
                     /locus_tag="P5541_05805"
     CDS             3529..4785
                     /locus_tag="P5541_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRV71532.1"
                     /translation="MGKTFAEKVLGKSAGESVSAGQVVIVEPHFCMSHDNAAPISKTF
                     KKIGVSKVWKPDHLVFILDHAVPAPTDKHAENHMQIRAFVKEQGIKHFYDVTSKGGVC
                     HQIMCEEGFALPGLVMVGSDSHTCTYGALGAFSTGIGRSEMAGAWATGQIWFRVPESL
                     KINVTGKFKKGVSAKDLILKIIGDIKADGADYMSVEFHGPAIEAMSIAERMTLCNMGI
                     EMGAKNAVCPPDKKVLDFIGPIAKTDAWEALWADDDAVYAQVLNYDLGDLEPCVAKPH
                     TVDNYAPVSEVKGTQIHQAFLGSCTNARIEDLREAAAVLNGKEVAVRTIVIPASWSVY
                     RQAMKEGLFDVFLDAGCVIGNPGCGPCMGNHEGILAPGETCISTANRNFKGRMGNKES
                     NIFLASPMTVAASALKGEISDPREVL"
     gene            4790..5281
                     /locus_tag="P5541_05810"
     CDS             4790..5281
                     /locus_tag="P5541_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRV71533.1"
                     /translation="MKITGKVWKYGDDVNTDVIFPGKYTYTVSDPAEMGQYALEDLDQ
                     DFTKNAKPGDIIVGGKNWGCGSSREQAVTCIKMRGIGAIIAKGFARIYYRNALNEGLP
                     IVVSAEAVDALEKGQEVTIDFDKSEIVTPKGTFSFPPYPDFVKGLIEDGGLIPHVKKS
                     LGL"
     gene            5765..7000
                     /locus_tag="P5541_05815"
     CDS             5765..7000
                     /locus_tag="P5541_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRV71534.1"
                     /translation="MARNVMQVKEKKDRYQVAYIPGDDSGFDVMEAAMIVMQALDAPI
                     DWVRADMGWCMWEKSCKEFPEGDKRRNTVPQDTIDKVRKADAALMAAITSKAGVKGFK
                     SAILQMRQIFDLYINFRPAKLLPGLECPLKGDPKIDIKMFRENTEDLYAAVEWRPLPK
                     EMFDLHPGMERFKDKSEVAVSWRVYSKEGCDRIIRAAFEYAKANGLKSVVCCNKANVI
                     RATDGMMKERFLEIAKEYPDIESREENADATAMWIIKNPQDYQVVVTSNVFGDILSDE
                     ASQLVGGMGFAPSGNIGLEAAIFEPSHGSVPKYAHQYKINPCGLLAAAVMMLDYLGLK
                     EHAARLEKAMGDVLVEGKSLTYDIWRDDKADKDWEKKAVSTLEMAKAIAGKIDPGFDK
                     KADAICAKAREMCAWEHLA"
     gene            7310..7585
                     /gene="citD"
                     /locus_tag="P5541_05820"
     CDS             7310..7585
                     /gene="citD"
                     /locus_tag="P5541_05820"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HRV71535.1"
                     /translation="MKTAQAGTLESMDCIVTVSESEKGRGITVSMEGASIARFGEAIV
                     SSTKDTARKLGIRDVSVSIQDNGAGDLVLRARVEAAIKRLTAGGDAL"
     gene            7582..8463
                     /locus_tag="P5541_05825"
     CDS             7582..8463
                     /locus_tag="P5541_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRV71536.1"
                     /translation="MRLRRTKLYLPGNNPNMLLRGHLFEPDGVILDLEDAVAMSEKDS
                     ARILVRDVLKRGDFGNCEVTVRINGMDTDLWRTDIETVVPCGIDGIRIPKVENPKDMA
                     LMDEALSEIESKAGIPAGRTLVFCLLETALGIWKAFDIATASKRIAAICPGGEDLRAD
                     LKTTRSENSEELIGPRRMVVLAAHAAGVDALDTVYANITDDAGLRRETEWVKQLGFQG
                     KSVIHPNQIQVIHDVFTPTAKEIEQAKKIVDAAADAASRGLGAVQVDGKMVDKPVVRR
                     AEYTLHRAGLKSGGEKQ"
     gene            8460..10010
                     /gene="citF"
                     /locus_tag="P5541_05830"
     CDS             8460..10010
                     /gene="citF"
                     /locus_tag="P5541_05830"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRV71537.1"
                     /translation="MTVNAAGRNIPDSIPGYGEVNHFQGHDKRTPEGNRYGKKNRASF
                     NSKTDKILPDIRAAISASGLESGMTVSFHHHLRNGDYVVNAVMDACAEMGIKDLVIFP
                     TALFGVHKHLTRHIQSGVIRQIQGSVNGPIGQLASEGGMEVPVVLRSHGGRPRAVTSG
                     DVVIDVAFIAAPTSDRYGNISGAVGKSACGSLGYAHSDAEYAECVVAITDNLVQYPAP
                     NIHIPQYYVDYVVPVESIGDPAGIVSGTTRVTRDPMRLLIARNAAKLIESSQYFRNGI
                     SFQTGAGGISLAVAAYVREAMRERGIKGSFGLGGITEFFVDMMKEGLFERLMDVQSFD
                     LAAVKSIGENPNHIEISADWYANPWNAGCAVNMLDTVVLGATEVDRNFNVNVNTEADG
                     ALLHGIGGHQDTAAGAQLTIITTPLLRGRIPCAVDSVYTVTTPGEVVDAIVTEFGVAI
                     NPKRQDLIDSAREAGDVPLVSMDELIEKAKGLTGNMSPVETDERIIGVVEYRDGTVID
                     VVRQLKKK"
     gene            complement(10330..11655)
                     /locus_tag="P5541_05835"
     CDS             complement(10330..11655)
                     /locus_tag="P5541_05835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRV71538.1"
                     /translation="MRVCIIGTGYVGLVTGACLAENGNDVWCVDVDRKKIEGLREGRI
                     PIYEPGLEDIVRRNVSQNRLRFTSDLKEGLDDSLFVFIAVGTPPMEDGSADLGYVFAA
                     AAQIGTLMDGYKIIVAKSTIPVGTTERVKEIIDEKLRERKSDIEFDVAFCPEFLKEGA
                     AVEDCLRPDRIVIGTSNERTAEFLKELFAPFVMKENRFYTMSIPSAEMTKYASNAMLA
                     TRISFMNELSRLCEKVGADVDQVRVGMGTDSRIGSSFLYAGLGYGGSCFPKDVQALIH
                     CAASSGVDLSILKAVEETNREQRRIFIRKITGHFGEDLSEKVFALWGLSFKPHTDDMR
                     EAPALTIAEALLSRGGSIRAFDPVAMDRAKELLPEDGSVIFCDDAYEALDGTSALVLV
                     TEWPQFRRPDWDRIKELMKPDSAVFDGRNQLSPARVRELGFSYYGIGRV"
     gene            complement(11780..12145)
                     /locus_tag="P5541_05840"
     CDS             complement(11780..12145)
                     /locus_tag="P5541_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRV71539.1"
                     /translation="MDKLGMLMILGSALFNAAASSMMKTGFGNQHDLLEKGFWGAVSR
                     IATNPWAIAGVVFFGISFMFMSAALTRVDLSVAYPVMSGLVFVLVLAVSVFFFSEQVT
                     PIRLMGVFLILSGVLAISR"
     gene            12259..13920
                     /locus_tag="P5541_05845"
     CDS             12259..13920
                     /locus_tag="P5541_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV71540.1"
                     /translation="MFSSHRKNILFLLILTTILYLVALGSHGLLDPDEGRYSEIPREM
                     IESGDYITPRLNYVAYFEKPVLHYWLTALSFKVMGQNEFAGRFWPAIMALGGVFVTYW
                     LANRMNGARSAFLSAWILATSLVYFAIGQINITDMPLSFFMTLSLAGFWMGHRRDRRF
                     FLLFYAGMALATLTKGLIGIVLPAGVTFWWIVLTRKWSVIKDTLYLPGIALFFLIGTP
                     WFVLVCLENPDFFNFFFIQEHFLRYTTKMHGRFEPWWWFIPILLVGLIPWTGFIPGAV
                     KKAFPSTLSKPTHQERGNLFLLLWFAIIFAFFSLSSSKLIPYIIPVIPPLAILAGNFL
                     DGLMDRGKTRELFMGMLFNTMVLLPFGIALAAYPFLDQRYGTMLLPFVAPVAAVLIFL
                     VILSWIFYARGESRKVVLTLCIFAFLNIATFKNVFGFYDRIMTARDVAATIREYILPG
                     DIVAQYREYDQGLPFYLKQRIVLVEWVGELEFGSKRGDQSDWFLDTQGFLERYWKSDE
                     RVILVMREEQPLEFLEETGVTPVILFRSDRKIVVANKEMSSADEK"
     gene            13910..15076
                     /locus_tag="P5541_05850"
     CDS             13910..15076
                     /locus_tag="P5541_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRV71541.1"
                     /translation="MRSEFLPFARPDISEEAIADVVDSIRSGWITTGPKTQRFEKSFG
                     EYVRSPYSMAVNSATAGLHLAMLAFGIGPGDEVITTPMTFAATVNTIVWVGAKPVLAD
                     IDPVTLNIDPQNIERVLTPRTRAIVPVHFAGRPCRMDEIESIAHDHGLVLIEDAAHAL
                     GAEYRGRRIGTALGDTHAAIFSFHPTKNITTGEGGMLCTGNEEIAEKVSVLRQHGMSK
                     GAWNRYAVTGNPHYDILFPGLKYNMLDIQAAIGNDQLLHLEKFNNRRRQIVDRYRREL
                     GGLDTLTLPGESGKGDIHSWHIFTPLVMIEKTGFSRDEFMARMREKNIGTALHYQAIH
                     LFSYFADNFGWKRGDFPNAEYVSDRIVSLPLFPAMTDDDLAEAIEATREICGRR"
     gene            15080..16021
                     /locus_tag="P5541_05855"
     CDS             15080..16021
                     /locus_tag="P5541_05855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV71542.1"
                     /translation="MEEKNMEISVVIPVYNEEESLPPLFERLFPVMESLGREWEVIFV
                     NDGSRDRSLGILLGCVDRFPGRVKVVDFNGNFGQHMAITAGFSASRGQVVVTMDADLQ
                     NPPEEIPRLLAETDRGHDVVGTIRSMRKDPFFRKFASKIVNRITNQITGLNLNDYGCM
                     LRAYDRRVIDLINGCGETTTFIPALAQRFSLNPVEIEVGHSERETGTSKYSLFRLIRL
                     NFDLMTGFSLFPLQAVTMLGLLVAGFSFIFTIYMILRRIMVGPEAEGLFTLMTINFFL
                     LGVTMFCVGIAGEYIGRIFQEIRKRPRYVINRIYGDE"
     gene            16014..16883
                     /locus_tag="P5541_05860"
     CDS             16014..16883
                     /locus_tag="P5541_05860"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HRV71543.1"
                     /translation="MNERPRTVVFAYSEVGHRCLGALIRSGANIRGVFTYKDDPGENT
                     WFGSVAELAEENGIPVFTDVDLKSTGGGQLVRELDPEILFSFYYRDMIPRSILEIPRL
                     GAFNMHGSLLPKYRGRACVNWAIIHGETETGPTLHWMTVRPDEGDIVDQEKVSIEPED
                     TAMTVMLKIAGAAETILLRNLTLIEAGKAPRTPQDHSRATYFGGRKPEDGRLDWNATA
                     KEICDLVRAVTHPYPGAFTDIFGDRMFIWKATPLEDDTVHPPGTTISREPLVIAAGKG
                     AVRVDEKSQERNG"
     gene            16978..18012
                     /locus_tag="P5541_05865"
     CDS             16978..18012
                     /locus_tag="P5541_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HRV71544.1"
                     /translation="MNILILGANGFIGSHLCEHLLKNTEWTIQGFDIKDFNLGNCLGK
                     DNFSFRKGDFYADDEWIEEAVNSCDAVLPLVGIAKPAFYITNPVMTFHLDFEANLKIV
                     RMCVKHGKRVIFPSTSEVYGMSTDKELKEDESPIILGPVCKSRWIYSCSKQMMDRVIA
                     AYGQEEGLRYTLFRPFNWIGSRLDTFTDARDHKARSITQILYDILNDREISLVGGGRQ
                     RRSFTWIGDGVKCLEAIIRNDNKCADGEIFNIGNPANNASIKELALMIIRVLKEFPQH
                     AEKAERAQLREIDSRDYYGKGYEDVQDRVPSVAKAKLLLGWEPETMFEEAVKKTLEAT
                     LRENDLQVVS"
     gene            18043..19425
                     /locus_tag="P5541_05870"
     CDS             18043..19425
                     /locus_tag="P5541_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRV71545.1"
                     /translation="MNIPANIFREYDIRGNAEEELTSNNVRAIGLAFGTFLAREGIRK
                     VTVGGDIRLSTERIRHDIIAGLTSAGIDVVNLGTVTSPLLYWSLFHLNLGGGVMITGS
                     HNPKEFNGLKLAFGKTTLYGDQIQEIRRMIQADDLIASSRPGEVTDLDLTEDYLGMLR
                     SKITLGPRKLKVVTDPANATAGPMVNRFLRDIGCDVIPLFEEPDGTFPNHHPDPTKRE
                     YLVDLIETVRREGADLGIGFDGDADRIGVVDNNGDVVWGDILMILFWREILPLHPGAD
                     AIVEVKSSQALVDEISTLGGNVVWWKSGHSLIKAKMKELDALFSGEVSGHMFFADEFY
                     GFDDSFYAAGRLLRILSNTEKKLSEILSDVPVYYSTAETRINCPDDKKFGVIERIRED
                     ALKDHEAITVDGVRIIYPNGWGLVRASNTQPVIVARAEGKTREELKEYSGDLKKRILN
                     AGVANFDWEY"
BASE COUNT         5047 a         5206 c         5255 g         3939 t
ORIGIN      
        1 gggggggtac cgaaacgatt tttttccagg gtgacttaaa gtaaagaacg cactgctacc
       61 gcagagttcc tgcggagcac atgctcagat cggaccgact gcagtacttc agggcattcc
      121 agttgaagag ttgcgaaaag ttgagaactt tacttttcgc catgtatggc cggtctttat
      181 accccccggt tccggttgat ggtaacgtaa acatggtcat gtgtcaactt tttgtatgca
      241 ctaaagaaaa tgtcccccgt gtctgaacat ttagtcttct tgacacccct tgcacaaatg
      301 gtctaccggg tatataaagt gtactgggtg gtccacagag gaggtaagaa atgaccgaca
      361 gaaaactgag gcatacaagg atatacgaac aggtcgtcaa cgagcttaag gagttgattg
      421 cttcgggaga actccagcct ggtgacccac ttcctcccga aagacagatg atggcccaga
      481 tgggcgtgag cagaagttcc ctgagggagg ctttcagggt gatggaactc ctcggtctga
      541 tagacagcgt cccgggtaag gggaggttcg tacgcaaacc gcggggagag acggccgatt
      601 ccaggaacat gcccctggag gacgaagccg tcctggagct catggaagcc cgcagggttc
      661 tggaccccgc tatcgctgca gccgcggcaa tgcactcctc cccttcggat ctgatgaagc
      721 ttcgcaaggt gctctcccgg accgagaaga atatcgagaa cgttggcaaa cggtcgcagg
      781 acgatttcga ctttcacctg gcacttgcag agtctaccca caattttgtt ttcgtaaaca
      841 tagtaaagat gactttcaac ctgatcatgg ctacccacga ccggatttat tcccttctgg
      901 ccgacaagga agccttcctc aaggagcacc aggaaattta cggcgcaata atagatcacg
      961 acgtggcaag ggcccggacc ctgtcggcca gccatatcga aagggtctac aagactctgc
     1021 aggaagcact ggccctcgag gtcaagtagc aataaagagc ggcaaggctt cgaaataaat
     1081 ttctggaggg atgttcgcat gagagacagg atcatcaaaa tgatccccga gatcgattgg
     1141 atccaggaca aggacctcag gacaaaagtc gtagcgaccg tcgaggatgc cctgaagacc
     1201 ggtggatggg agccggaaga catggagaag atccccttta cgcttctgat cccggactgc
     1261 cccgcatcat tgaacgttca caacaggggc gttacgagaa tggcaaaaaa gatcttcgag
     1321 gaatacaact ctctttacaa ggacaacggt ggattccagc ttgaccacga cacccttatc
     1381 gccggcgcca tactacacga cgtgggcaaa cttgtggagt acgagaagaa ggacggcaag
     1441 gctgtgaagt cccagatggg caaggacctc cgccacccct tctcgggcgt cggcctggcc
     1501 atgcgcaacg gtataccttc cggtatatgt cactgtatcg ccgtccacgc caaagagggt
     1561 gacggaggcc atcgttctcc tgaggccgtc gtcatcaaca agtgcgattt catcaacttc
     1621 gagacgatca agtcgttcct gggactgctt tagcattaaa gaaaatattc gggaggtgcg
     1681 tgacccatgg gcaaaactat gatcgaaaag atcatgaagc gggccgcagg acgtgaagtc
     1741 aacgtaggag aaagagtctg gtgcaaaata gacctttcca ctgcccgcga cttcggaggc
     1801 gccaactgcg tccttcagtt cgaagaggtt accgacaaaa agggcaaagt ctgggatcct
     1861 gacaaggtag ccttcacttt cgatcttcag gctccgtcac attcggaaaa agtttcaacc
     1921 aaccagaaga taattcgcga ttttgccaaa aagcagggca tcaaaaaagt ttttgatatt
     1981 aactggggca tcggccagca tgtcatgctc gagaacggac tggtcaaacc cggtgatgtc
     2041 atccttggaa cagacagcca catgaacctg ctcggcgccg tgggtgcttt cgccaccggc
     2101 gttggcaaca ccgacatagt ggcctcgtgg ttcgagggga ctaactggtt ccgggttcca
     2161 gagaccatga agataaccgt taacggttcc ttcaaaaaag gggtctgtat gcgcgatctc
     2221 cttaccagca tcgtaggaac ccttcacgcc gacggaatgt tctttctctc cgtcgagttc
     2281 tatggggaca ccatagagaa gtcttccctg gccgacagga tcaccctctg ctcaatggtg
     2341 acagaaatga gcggaaagat cgggctcatc atgcccaacg gcaaggttct ggactggctc
     2401 gtggaacgtg cgggaaaaga ggtccttgaa cgtgtaagca gtcttgcggc agatccagat
     2461 gccgtgtact gccaggaact ggaattcaat gtaaatgacc tggagccgat gacatcctgc
     2521 cccgatgctc ccgacaacgt gaagaaggtt cgcgaagttg ccggtgccca cgtcgaccag
     2581 gtccacatcg gctcctgctc caacgggagg ttcgaggaca tcaccgcagc tttcgaagtt
     2641 ctgaaggctg ccaacttcag gatcgccccc ggaatccgcg ccataatcac tccgtcgact
     2701 cgtgaagtaa tgcttcagtg cgccgaagcc ggctatatcc agaagttcct tgaagcggga
     2761 gttgtcttca ccaacccgac atgcgcacta tgcactgccg agcactacgg ggcccttccc
     2821 agcggtgacg tgggcatgtc aaccacaaac aggaacttca tcggcaaggt cggcaaggga
     2881 agccacacct accttgcaag tccgatgacc gccatggcca cggccgttcg cggcgttgtg
     2941 accgatccca gggatatcct cggataggga ggggaagaga aatggacaag acaatccttc
     3001 gtggaaaggc ctgggttttt ggagatgaca tcgacactga cctcatctac cacaacaaat
     3061 acctggccga gacagacccg aagaacatgc cgcagtactc tttcgagtac ctgcctggca
     3121 aggagaattt cgccaaagag gtaaaagagg gagattttgt tgtagcgggg accaacttcg
     3181 gttgcggatc gtctcgtgag catgccgtat actgcctgaa atatgccgga gtaccggtaa
     3241 ttcttgccga gtctttctca cgcatctact atcgcaacgc catcaacaac ggttaccccg
     3301 tccttttcgt caaggggctg agccaggcga tcaaggacgg aaagatcaag gacggcgacg
     3361 aacttgaggt aaatcttctc aagggagaga tcaaagatgt cacgagcggc aacaccttcc
     3421 acggagatgc cgtagcggac cttgagaagg acatcatgaa ggccggtggc ctcatagagt
     3481 acctcaagga aaaggccgcc aaaaagtaga gagggggaca gatcctaaat gggcaagaca
     3541 tttgcggaaa aagttctggg caagtccgcg ggagagtctg tatcggcggg ccaggtcgta
     3601 atagtggaac ctcatttctg catgagccac gacaacgcgg cccccatctc caagaccttc
     3661 aagaagatcg gggtcagtaa ggtatggaaa ccggaccacc tggttttcat ccttgaccac
     3721 gcggtaccgg cacccacaga caaacatgca gagaatcaca tgcagatcag ggctttcgtg
     3781 aaggaacagg ggattaaaca cttctatgac gttaccagca agggcggggt ttgtcaccag
     3841 atcatgtgcg aagagggatt cgcccttccc ggacttgtca tggtgggaag cgacagccac
     3901 acgtgcactt acggagcctt gggcgcattc tcaaccggaa tcggccgaag cgagatggca
     3961 ggcgcctggg caaccggcca gatatggttc agggtccccg aatccttgaa aataaacgtg
     4021 acgggcaagt tcaaaaaagg cgtgtccgcc aaggacctga tccttaagat catcggcgac
     4081 ataaaggccg atggagcaga ctacatgtcc gtcgaattcc acggtcctgc aatagaagcc
     4141 atgtcgatag ccgagaggat gaccctatgc aatatgggca tagagatggg tgccaagaac
     4201 gccgtatgtc ctcccgataa aaaggtcctg gattttattg gcccgatcgc caaaaccgat
     4261 gcctgggaag cgctctgggc agatgatgac gcggtatatg cccaggttct gaactacgat
     4321 ctgggcgacc tggaaccctg cgtggcgaaa ccccacacgg tggacaacta tgcaccggta
     4381 agcgaggtca aaggaacgca gatacaccag gccttcctcg gaagctgcac caacgcacgc
     4441 atcgaggatc tccgtgaggc agcagccgta ctcaacggca aggaagtcgc cgtaaggacc
     4501 attgtcatac cggcatcctg gagcgtctac cgccaggcaa tgaaagaagg gcttttcgac
     4561 gtatttctcg atgcgggctg cgtgatcggg aacccgggat gcggcccctg catgggcaac
     4621 cacgagggca ttttggctcc gggggagacc tgcatctcaa ccgccaacag gaacttcaag
     4681 gggcgcatgg ggaacaagga gagcaacata ttcctcgcaa gcccgatgac agtagcagcc
     4741 tccgccctaa agggcgagat ctccgacccc agggaggtgc tgtaaaacca tgaagatcac
     4801 cggaaaggtc tggaaatacg gggacgacgt caacaccgac gtcattttcc cgggaaaata
     4861 cacttacact gtctctgacc ctgccgagat gggccagtat gcccttgagg atctcgacca
     4921 ggatttcacc aaaaacgcaa aacccggaga cattatcgtg ggcggaaaga actggggatg
     4981 cggatcctcg cgcgaacagg cggtgacctg tatcaagatg cgcggaatag gggcaattat
     5041 cgccaagggt ttcgcccgca tctattaccg caacgctctc aacgagggac tcccgatcgt
     5101 ggtttcagcc gaagcagtcg acgcccttga gaaggggcag gaagtgacca tcgatttcga
     5161 caagagcgag atcgtgacgc cgaaggggac attctctttc ccgccttacc ccgattttgt
     5221 gaaggggctc atcgaggacg gcggcctgat cccccacgtc aagaagagtc tggggctgta
     5281 aagacaggcg acaggcaaca ggcgacagga ggcggggcaa acaagaaccc gcgagacgtg
     5341 agacgtgaga cggatggatc cgtaaaccgt gaatcgtgaa tcgttagtcg aaaaggtcta
     5401 aagcaaagac ctgaacggta aacggaaaaa cagaaactga aaaccttgaa tcgtgaactt
     5461 tgaatcgaaa accgcaaagg cctgaacggt gaacggggaa aaaccttgaa ccgttagaag
     5521 taagaagtga gaggacagga ccttgaactg ccggaagctt tactggaaac ataggagcct
     5581 gtgaggggac caggcgtcgc ggaggggcag ggacagcgca ggagtacgtt ttttggacga
     5641 ctttgcgcga tcgcaccgga gcagccgggt tcgcgaacga tctcaggcga gtcgtttccg
     5701 ggaaaatctt ccggcagcgc aaaaacccca gatcacgatc tacgaaaaat gaggaggcaa
     5761 taatatggcc aggaacgtta tgcaggttaa ggagaagaag gacaggtacc aggtagctta
     5821 tatcccgggg gatgactcag gcttcgacgt aatggaagcc gcgatgatcg tcatgcaggc
     5881 ccttgacgca cccatcgact gggttcgcgc cgacatgggg tggtgcatgt gggagaaatc
     5941 ctgcaaggag ttcccggagg gtgacaagag aaggaacacc gtcccccagg atacgatcga
     6001 caaggtcaga aaagccgacg ccgcccttat ggccgccatc acctccaagg cgggagtcaa
     6061 ggggttcaaa tcggccattc tccagatgag acagatattt gacctttaca tcaacttccg
     6121 tcccgcaaag cttctgcccg gccttgagtg ccccctcaaa ggggatccca agatcgatat
     6181 caaaatgttc cgcgagaaca ccgaggacct ttacgctgcc gtcgaatggc gcccgcttcc
     6241 caaagagatg ttcgacctgc accccggcat ggaacgtttc aaggacaaat ccgaggtagc
     6301 cgtatcctgg cgcgtttatt ccaaagaggg ctgcgacagg atcatcaggg ccgccttcga
     6361 gtacgccaag gccaacggcc tcaagagcgt cgtctgttgc aacaaggcca acgtcatccg
     6421 cgctaccgac ggcatgatga aggaacgctt cctcgagatc gccaaggagt atcccgacat
     6481 cgaatcccgc gaggagaacg ccgacgccac agccatgtgg attatcaaga acccgcagga
     6541 ttaccaggta gttgtcacct ccaacgtctt cggtgacatt ctctctgatg aggcatccca
     6601 gctcgtaggc ggcatgggct ttgcccctag cggaaacatc gggttggagg ctgcaatttt
     6661 tgaacccagc cacggctcgg tcccgaaata tgctcaccag tacaagatca acccctgcgg
     6721 cctgcttgca gcggcagtga tgatgctcga ttatctcggc ctcaaggagc acgccgccag
     6781 gcttgagaaa gccatgggcg atgttctggt agaaggcaaa tccctcacct acgacatctg
     6841 gcgcgacgac aaggccgaca aggactggga gaagaaggcc gtttcgaccc tcgaaatggc
     6901 aaaggctatc gccgggaaga tcgaccccgg ctttgacaag aaggccgacg caatctgcgc
     6961 gaaggcccgc gagatgtgtg cctgggagca tctggcttaa aaaccgtgag acgtgagacg
     7021 gaagggcaaa accaaaaggc gttaacagta aacggtcaac ggtaaacgga aaaacagaaa
     7081 ctgaaaacct tgaatcgtta actgtgaatc gaaaaccgca aagacctgaa cggttaacgg
     7141 gaatgcagaa cctgaaaacc tgcaataccg tgaatcgaaa atcgggaccg ggaaacaggc
     7201 aacctggaaa tagcaatgag cccggggctc accttcgggt gggccccgga cgcttcaaag
     7261 cggagaaagg caagatgaag tgcgacgctt catgcaggga ggtaagaaga tgaaaacagc
     7321 acaggctggc acccttgaat caatggactg tattgttacg gtaagcgagt ccgaaaaagg
     7381 gcgcggcata acggtttcca tggaaggggc aagcatagcc cgtttcggag aagccatagt
     7441 cagcagcacc aaggatactg caaggaaact cggaataagg gatgtatctg tatccatcca
     7501 ggacaacggc gcgggcgacc ttgtgctcag ggccagggtc gaggccgcaa tcaaaagact
     7561 gactgcaggg ggggatgccc tgtgaggctg agaagaacca agctctacct gccgggaaat
     7621 aaccccaaca tgctcctgag gggacacctt tttgagcccg acggcgttat ccttgacctt
     7681 gaggatgccg tagcaatgag cgaaaaggat tccgcgagga tactggtaag ggatgtactt
     7741 aagaggggcg atttcggcaa ttgcgaggtc accgtaagga taaacggaat ggataccgac
     7801 ctctggagaa ctgacatcga gaccgtcgtc ccctgtggga tagatggtat ccgcataccc
     7861 aaagttgaga acccgaaaga catggccctt atggatgagg ccctttccga aatagagagc
     7921 aaggcgggga tacccgcagg aaggaccctt gttttctgcc tgctcgagac agccctggga
     7981 atatggaaag ccttcgatat tgccacggcc tcgaaaagaa tagccgccat ttgccctgga
     8041 ggagaagacc tccgagccga cctgaaaacg acccgttcag aaaacagcga ggaattgata
     8101 ggcccaagga gaatggtcgt tcttgccgcg catgccgcgg gggtcgacgc tcttgacacg
     8161 gtctacgcaa acattaccga cgatgccggt cttcgcaggg agaccgaatg ggtcaagcag
     8221 ctcggttttc agggcaagag cgtgatccac ccgaaccaga tccaggtgat ccacgacgtc
     8281 ttcaccccca cagcaaagga gatcgaacag gctaaaaaga tagtggatgc agcggccgat
     8341 gcagcctcca ggggactggg agcggtacag gttgacggca aaatggtcga caaaccggtc
     8401 gtaaggcgtg cggagtacac cctgcataga gcaggactga agtccggagg tgaaaaacaa
     8461 tgaccgttaa cgcggcggga aggaatattc ccgactccat accgggctac ggggaagtga
     8521 accatttcca gggtcatgac aaaagaaccc ccgaaggcaa ccgctacggc aaaaaaaaca
     8581 gggcttcctt caacagcaaa accgacaaga tcctccctga tatcagggca gccatcagcg
     8641 cttcggggct tgaaagcggc atgaccgtat cgttccacca ccatctcagg aacggcgact
     8701 acgtggtcaa cgccgtaatg gacgcatgcg ccgaaatggg tatcaaggac ctggtgatct
     8761 tcccgactgc cctcttcggg gtccacaaac atttgaccag gcacatacag agtggggtta
     8821 tccgccagat ccagggctcg gtcaacgggc ccataggcca acttgcctca gagggaggca
     8881 tggaggttcc ggtggtgctt cgaagtcacg gaggacgccc cagagccgtc acaagtgggg
     8941 acgtggtcat cgacgtggcc ttcatcgctg caccgacatc cgacaggtac ggaaacatct
     9001 cgggcgccgt gggaaaatca gcttgcggat cgctgggata tgcacattct gacgcggaat
     9061 acgctgaatg cgtggtcgca ataactgaca atctagtcca gtaccctgcc cccaatatcc
     9121 atatacctca gtactacgtc gactacgtgg ttcccgtgga atccataggg gacccagcgg
     9181 ggatagtcag cggcacgacc cgagtaacca gggaccccat gaggcttctg atcgccagaa
     9241 acgcggccaa acttatcgaa tccagccagt acttccgcaa cggtatctct ttccagacag
     9301 gagcaggcgg catatccctg gcagtggcgg cctacgtaag ggaagccatg agggagagag
     9361 gtatcaaggg gagcttcgga ctcggtggaa taacggaatt tttcgtcgac atgatgaagg
     9421 aaggcctctt cgaaaggctt atggatgtgc agtctttcga cctcgctgct gtaaaatcca
     9481 tcggcgagaa cccgaaccac atagagatct ccgcggactg gtacgcaaac ccctggaacg
     9541 cagggtgtgc cgtaaacatg ctcgacacag ttgttcttgg agccacagaa gtcgatcgga
     9601 acttcaacgt caatgtcaac accgaggctg acggcgcatt gcttcacgga atagggggac
     9661 accaggacac cgccgcggga gcacaactga cgataatcac cactcccctg cttcgcggga
     9721 ggatcccctg cgccgtagat tcggtttata cggtcaccac acccggagag gtcgtcgacg
     9781 cgattgtcac cgagttcgga gttgcgatca accccaaaag acaggacctt atagattctg
     9841 ccagggaagc aggagacgtc ccccttgtct ccatggacga actgatagaa aaggccaaag
     9901 gccttaccgg aaacatgtcg cccgtcgaga ccgatgaaag gatcataggc gtggtggaat
     9961 accgtgacgg caccgtcatt gacgtggtta ggcagttgaa gaaaaagtaa taaaccggtg
    10021 aacggtgaca ggtgaacggt aaacggtaaa aacaataaca gcaagatcat atacaggaag
    10081 gccgggagtg ttcccggcct tcctgtaatt tgctttgtat gttctgctgt tgccatcttt
    10141 aaatggtttt gttcagaggt tcctttactg tttactgttc accgtttacc gttgacgccc
    10201 ttgaatttgg tttttcgagt cacgcgtcac gcttatgcct ttgctgtcgc ctgatgcctg
    10261 tctcctgacg cctctgtttt atgccttttc gtctcacgtc taacttctca cgccttagag
    10321 gtttccactc tacaccctcc ctatcccgta atacgagaac cccagctcgc ggacccgagc
    10381 aggcgacaac tggttgcggc cgtcaaatac agctgaatcc ggcttcatca gttccttaat
    10441 ccggtcccag tcaggcctgc ggaactgggg ccattcggtt acaaggacga gggcactggt
    10501 cccgtcaagg gcttcatagg catcatcgca gaatattacc gaaccatcct caggaagcag
    10561 ttccttcgcc ctgtccatgg ccacaggatc gaaagccctt atggatccgc cccttgaaag
    10621 aagcgcctcg gcaatagtta gtgccggtgc ttcccgcatg tcatcggtgt gaggcttgaa
    10681 acttaatccc cataacgcga agactttttc cgaaagatcc tccccgaaat gtcccgttat
    10741 tttccttatg aaaatgcgcc gctgctccct gttggtctcc tccacagctt tcagaataga
    10801 gaggtctacc ccactggatg cagcacaatg gatcagggcc tgaacatcct tggggaaaca
    10861 ggaaccgccg tatcccaacc ccgcatagag gaaagaggag ccgatccggc tgtcggttcc
    10921 catacccacc cggacctggt caacatcggc accaaccttt tcgcagagac gggaaagttc
    10981 attcataaaa ctgatccttg tcgcaagcat cgcgttggag gcatattttg tcatctcggc
    11041 agagggaata gacattgtgt aaaaacggtt ttccttcatc acaaaggggg caaacaattc
    11101 cttcaggaac tcggccgtcc tttcgttgga tgtccctata actatcctgt ctgggcgcag
    11161 gcaatcctct actgctgcgc cttccttgag aaactcgggg cagaaggcaa cgtcgaactc
    11221 tatgtcactt tttctttctc tgagtttttc atctatgatc tctttgaccc tttccgtcgt
    11281 cccgacaggg atggtggact ttgcgacgat tatcttgtag ccatccataa gggttccgat
    11341 ctgggcggcc gcagcaaaca cgtagcccag gtctgcggat ccatcctcca ttggcggagt
    11401 ccctacggct ataaaaacaa aaaggctgtc gtcaagtcct tccttgagat cggaagtaaa
    11461 gcgcagcctg ttctgggaaa cgttccttcg aacaatatcc tccaggccag gctcatatat
    11521 tggtatacgc ccctcacgga gaccctcgat cttcttcctg tcaacgtcaa cacaccagac
    11581 gtcgttgcca ttctcggcaa gacatgcccc tgtgacgaga ccaacgtaac cggtaccgat
    11641 gatgcagact ctcatgatgg acctcccaaa agcgtgagat taaagatacg tgagacgtga
    11701 gacgttagac gtgagacgaa aaatcagaga aaaatgccgt ggatcgtgga tcgaaaaatt
    11761 caaaggcgta aacggttttc tacctgctta tcgccagtac ccctgacagt atcagaaaca
    11821 ctcccatcag cctgataggc gttacctgct ctgagaagaa gaagaccgac accgccagta
    11881 caagaacaaa gaccagacca gacatcacag ggtaggccac agaaaggtct acccgggtca
    11941 gggcagcgct catgaacatg aaagatatcc cgaaaaacac cacacccgcg atcgcccagg
    12001 ggtttgtcgc aatgcgggaa accgcccccc aaaacccttt ttccaacaga tcgtgctggt
    12061 tcccaaagcc agtcttcatc atactgctcg ctgcggcgtt gaacaaagcg gatccgagga
    12121 tcatcagcat ccccaacttg tccaaaaaac attcctcccc ctggatggga taaactgaac
    12181 attaccgcta taataatcca gacccgaata ccatggaccc aaagggccaa agattttcaa
    12241 tacctggagg tagttctatt gttctcttcc cacaggaaaa acatactgtt ccttctgatc
    12301 ctgacgacaa tactatacct ggtagccctc ggcagtcacg gactcctcga tcccgacgag
    12361 ggacgctact cggagatccc tcgcgaaatg atagaaagcg gagactatat tacaccaagg
    12421 ctgaactatg tggcctactt cgagaagccc gtccttcact actggcttac ggcgctctcc
    12481 ttcaaggtaa tgggacagaa cgagttcgcg gggcgattct ggccagcgat aatggctctt
    12541 gggggggtat tcgttacata ctggcttgca aacaggatga acggggcccg atcagcgttt
    12601 ctttctgcct ggatactggc aacttccctt gtctattttg cgatcggcca aatcaacatc
    12661 accgatatgc ccctatcctt tttcatgacc ctttcgctgg cgggattctg gatgggacac
    12721 cgccgggaca ggaggttctt cctcctcttc tacgccggca tggcccttgc caccctgacg
    12781 aaggggctta taggcatagt cctccctgcc ggagtcacct tctggtggat cgtgcttacc
    12841 cgtaaatgga gcgtgataaa ggacaccctc taccttccag gaatagccct tttcttcctg
    12901 atcgggacac catggttcgt cctggtatgc cttgaaaatc ccgatttctt caacttcttc
    12961 ttcatccagg aacacttcct gagatacacc acgaaaatgc atgggcgttt cgaaccctgg
    13021 tggtggttca tcccgatcct gctcgtgggg cttatcccct ggacaggatt cattcctggg
    13081 gctgtcaaga aggcattccc ctcgacgctg tcaaaaccga cacaccagga gaggggtaat
    13141 ctttttctcc tcctatggtt tgcgatcatc tttgccttct tctctctctc aagttcaaaa
    13201 ctgatcccct acataatacc cgtgatccct cccctggcca tcctcgcggg caatttcctc
    13261 gacggcctca tggaccgggg aaagaccagg gaactcttca tgggaatgct cttcaacacc
    13321 atggtccttc tccccttcgg gatagccctt gcggcatatc cgttcctgga ccagaggtac
    13381 ggtacaatgc ttctcccctt cgtggctccc gtcgccgcag tactcatctt cctggttatt
    13441 ctttcctgga tcttttacgc caggggagag tcgcggaagg tcgtcctgac cctgtgcata
    13501 ttcgcgttcc ttaacattgc cacattcaag aacgtgtttg gcttctatga ccggatcatg
    13561 acagcacggg atgttgctgc cactataaga gagtatatcc tccccggaga catagtcgcc
    13621 cagtacagag aatacgacca gggcctcccc ttctacctaa agcagagaat tgttcttgta
    13681 gaatgggtag gagaactgga attcggctca aaaagagggg accagtcgga ctggttcctg
    13741 gacactcagg gttttctgga gagatactgg aaatccgacg agagggtaat cctggtgatg
    13801 agggaggaac agcctcttga gttccttgag gaaaccggcg taactcccgt aatcctcttc
    13861 cgctccgaca ggaaaatcgt agtcgcaaac aaggagatga gttctgccga tgagaagtga
    13921 gttcctgccc ttcgccagac cggacataag tgaggaagcg atagccgacg ttgtcgactc
    13981 cataaggtcg ggatggataa ctaccgggcc caagacacaa cgttttgaaa agtccttcgg
    14041 ggagtacgtc cgatccccct actccatggc cgtcaattcc gctaccgcgg ggctgcatct
    14101 ggccatgctc gctttcggga tcggccccgg agacgaagtg atcacgactc cgatgacatt
    14161 cgccgcaaca gtcaacacta tcgtttgggt gggagcaaaa ccggtacttg ccgatattga
    14221 cccggtcacc cttaacatcg atccccaaaa tatcgaaagg gttctaactc cgaggaccag
    14281 ggccatcgtt cctgttcatt ttgccggaag accctgcaga atggacgaaa tagaatccat
    14341 agcccatgac cacgggcttg ttttgataga ggacgccgcc catgcccttg gagcagaata
    14401 caggggaaga cggataggga cagccctcgg agacacccac gccgccatat tcagcttcca
    14461 ccccacaaag aacatcacca ccggcgaagg tgggatgctc tgcaccggga acgaggaaat
    14521 agccgagaag gtctcggttc tccgtcagca cgggatgagc aagggggctt ggaaccgcta
    14581 cgccgtaacg ggcaacccac actacgacat tctcttcccc ggcctcaagt acaatatgct
    14641 ggacatacag gcggcgatcg gcaacgacca gctgcttcat ctcgaaaaat tcaacaacag
    14701 aaggagacag atagttgaca gatatcgccg ggaacttgga ggtctggaca cccttaccct
    14761 tcccggggaa tccgggaagg gcgatattca ctcatggcac atctttaccc ctcttgtaat
    14821 gatcgaaaaa acgggttttt cccgtgacga attcatggca agaatgaggg agaaaaacat
    14881 cggcacggcg ctccactatc aggctatcca cctcttttca tacttcgccg acaattttgg
    14941 atggaagagg ggcgattttc caaatgccga gtacgtcagc gacaggatag tctctcttcc
    15001 tcttttcccg gcaatgacgg acgacgacct cgcggaagca atagaggcca ccagagagat
    15061 ctgcgggcgg agatagacta tggaagaaaa aaacatggaa atttcagtcg tgattccagt
    15121 ctacaacgag gaagaatctc tccccccgct ctttgaacgc ctttttccgg tcatggagtc
    15181 actgggaaga gaatgggaag taatattcgt gaacgacggc agcagggacc gctccctggg
    15241 gatcctgctg ggttgtgttg accgctttcc cggaagagta aaagttgttg atttcaacgg
    15301 aaatttcgga cagcacatgg ccataaccgc ggggttctcc gcctcgaggg ggcaagtcgt
    15361 ggtaaccatg gacgccgacc ttcagaaccc ccccgaggag atcccgaggc tgcttgccga
    15421 gaccgacagg ggacacgacg tggtcggcac cataagatct atgcgaaagg accctttctt
    15481 ccgcaaattc gcgtcgaaga tcgtcaacag gataaccaac cagatcacgg gcctcaacct
    15541 caatgactac ggctgcatgc tccgggccta tgaccgaagg gtcatagacc ttatcaacgg
    15601 gtgtggagaa accaccacct tcatcccggc cctggcacaa aggttctccc ttaaccccgt
    15661 agagatcgag gtaggacatt ccgagaggga aacaggcaca tcaaaataca gcctcttccg
    15721 actgatccgt ctgaatttcg atctcatgac aggtttttcg ctctttcctc ttcaggctgt
    15781 gacgatgctg gggcttctgg tcgcggggtt cagcttcatc tttaccattt atatgattct
    15841 gcggcgaatc atggttggac cagaggcgga gggactcttc actctcatga ctatcaactt
    15901 tttcctgctt ggtgtcacca tgttctgcgt gggcatagcg ggagaataca tcggcagaat
    15961 atttcaggag atacggaaac gcccccgata tgtcataaac aggatatacg gcgatgaatg
    16021 aaagaccccg gacagtcgtt tttgcctaca gcgaggtggg acacaggtgc ctcggagcgc
    16081 tgatccggtc aggagcaaac atcaggggcg tcttcaccta caaggacgac cccggcgaga
    16141 acacgtggtt cggctccgtt gccgaactgg cagaagagaa cggaatcccc gtatttaccg
    16201 acgtggatct gaaatcaacc ggtggggggc aactcgtccg ggaactggac ccggagatac
    16261 tcttctcctt ctactacagg gacatgatcc cccgttcgat cctggagatc ccgcgactgg
    16321 gagctttcaa catgcacgga tcactcctcc ctaaatacag gggaagggcc tgcgtgaact
    16381 gggcgataat tcatggagaa acggaaacgg gcccgacgct gcactggatg acagtacgcc
    16441 ccgacgaagg ggacatcgtc gatcaggaga aagtttccat agaacccgaa gatacagcga
    16501 tgacggtaat gctgaagatc gccggagcag ctgaaacaat actcctgagg aacctgactc
    16561 ttattgaggc tggcaaggca ccgcgaacac cccaggacca ttccagggcg acatattttg
    16621 gcggacggaa acccgaggac ggccgactgg actggaacgc gacggcaaaa gagatatgcg
    16681 acctggtgcg agccgttact cacccctacc ctggagcctt caccgatatt ttcggggaca
    16741 ggatgttcat ctggaaggca acacccctgg aagatgacac cgtacacccc ccggggacaa
    16801 caatatcgag agagcccctt gtcatagccg cgggaaaagg cgcggtaagg gtagacgaga
    16861 aaagccagga gcgtaatggg taagttttaa ttgttttttc cgttcactgt tcactgttta
    16921 ccgttcacgc tttttgagtt ctagattcac gatccacgaa aacggggagg tttcaatttg
    16981 aatattctta ttctcggcgc caacggattt ataggatcgc atctttgtga acaccttctg
    17041 aagaacacag aatggacgat ccagggcttt gacataaagg atttcaacct cggaaactgc
    17101 ctggggaaag acaatttttc ctttcgaaag ggcgatttct acgccgatga cgaatggata
    17161 gaggaagccg taaatagttg tgacgccgta ctcccactgg ttggaattgc caaacccgcc
    17221 ttctatatta cgaacccggt catgactttc cacctcgact tcgaggcaaa cctcaagatc
    17281 gtccgaatgt gcgtgaaaca cggcaagcgt gtaatcttcc cgtccacatc ggaggtctac
    17341 ggtatgagca ccgacaagga gctcaaggag gatgaaagcc ctatcatcct tggccccgta
    17401 tgcaaatcca gatggatata cagttgcagc aagcagatga tggatcgtgt catcgcggcc
    17461 tatggccagg aagaggggct ccggtacact ctcttcaggc cattcaactg gataggctct
    17521 cgactggaca ccttcaccga tgccagggac cacaaggcca gatcgataac acagatcctt
    17581 tacgatatcc tcaacgaccg cgagatatcc cttgttggtg gaggcagaca gaggagaagt
    17641 ttcacctgga tcggtgacgg ggtcaaatgc ctggaagcca ttatcaggaa cgacaacaaa
    17701 tgcgccgacg gggaaatatt caacataggt aaccccgcca acaatgcctc catcaaggaa
    17761 ctggccctca tgatcatcag agtcctcaag gaatttccgc aacatgccga aaaggcagag
    17821 agagcgcagc tcagggagat cgactccagg gattactacg gcaaggggta tgaggacgtg
    17881 caggaccgcg ttccttcagt agccaaggcc aaattgctcc ttgggtggga accggagacc
    17941 atgttcgagg aagcggtcaa aaaaactctg gaggccaccc ttcgggaaaa cgacctccag
    18001 gtggtatcat gaaaaaaaga ttttatcagg ggaggtcgcg ccatgaatat tcctgccaac
    18061 attttcaggg agtacgacat caggggaaac gctgaagaag aactcacgag caacaacgtc
    18121 agggcgatcg ggctggcttt cgggaccttc ctggcaaggg agggaatcag gaaggttact
    18181 gttggagggg acataagact ctccacggag aggatccgcc acgacataat tgcaggactt
    18241 acatcggcgg gtatcgacgt ggttaacctc ggaaccgtaa caagccctct tctctactgg
    18301 agcctctttc acctgaacct cggcggtggt gtaatgatca ccggcagcca caaccccaag
    18361 gaattcaacg gtctgaaact ggccttcggc aagacaactc tctacggtga ccagatccag
    18421 gaaattcgcc gaatgataca ggccgacgat ctcatcgcct cttcccggcc gggagaggta
    18481 actgatctgg atctcacaga ggattacctt ggaatgctcc gatcaaaaat aactctcggg
    18541 ccacgcaaac tgaaggtcgt aacggacccc gcaaacgcta cggccggacc aatggtaaac
    18601 aggttcctca gggatatcgg atgtgatgtc atcccgctct tcgaagaacc tgacgggact
    18661 ttcccgaacc atcatccgga ccctacaaaa agggaatacc ttgtagatct gatcgagacc
    18721 gttcgccgtg aaggagcgga cctcggtatt ggttttgacg gtgatgccga ccgcattgga
    18781 gtggtcgaca acaacggaga tgtggtctgg ggggacatcc tcatgatcct cttctggaga
    18841 gagatccttc cactccatcc cggtgcagac gccattgtcg aggtcaagag ctcgcaggct
    18901 cttgtcgatg agatcagcac cctggggggc aacgtggtct ggtggaaatc cggtcactca
    18961 ctgatcaagg caaagatgaa ggaactggac gccctgttct cgggcgaggt ttcgggacac
    19021 atgttcttcg ccgacgaatt ctacggtttc gacgactcct tctacgcggc gggcagactt
    19081 ctgaggatac tctccaacac cgaaaagaaa ctgtccgaga tcctttcgga cgtccctgtc
    19141 tattattcaa cagccgaaac aaggatcaac tgccctgatg acaaaaaatt cggggtcatc
    19201 gaaaggatac gggaggatgc cctgaaagac cacgaagcca taactgttga cggggtacgg
    19261 ataatctacc cgaacggctg gggacttgta agggcctcga acacccagcc cgtaatagtc
    19321 gcaagagcag aaggcaagac ccgcgaggaa ctgaaggaat actccggcga ccttaaaaaa
    19381 agaatcctta acgcaggtgt cgccaatttc gactgggaat attaaataac ttaaattcgc
    19441 gaatcgg
//
LOCUS       DAOTIQ010000036        19208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3426_length_19208_cov_7.294053, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000036 DAOTIQ010000000
VERSION     DAOTIQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 19208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19208
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_19208_cov_7.294053"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1082)
                     /locus_tag="P5541_05875"
     CDS             complement(84..1082)
                     /locus_tag="P5541_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV71546.1"
                     /translation="MVKDTTKHRTEHINHGQENEQDHSGHHHHDVSDTSDRRLAISLA
                     LNIGITIAEIVGGILSNSLALLSDAVHNFSDASSLGVSWLARRISRMDRTPNHSFGFK
                     RAEILASLVNTVALMGIGAFLMVESIKKFLHPEPVVGRIMLVVAIVGLLGNLVTAWLL
                     HSDSKKSLNIRAAYLHIVMDTLSSVGVIAAALLMMAYGWFWLDPMLTLLVSLYVLRES
                     WPLLKTTIHILMQGTPGNINARKLIDRLEESPDVLDVHHVHLWTTDGTDIFLEAHVSL
                     CSDPRPDTDIVIGNLSEIASSEFGIDHVTFQPEFARCVGGRCSGLDTCNREGSTID"
     gene            complement(1101..2270)
                     /locus_tag="P5541_05880"
     CDS             complement(1101..2270)
                     /locus_tag="P5541_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRV71547.1"
                     /translation="MNSDLKKFLYIAGAFLAFYFMPIESTRLHRAAIEALAMMHEYAR
                     EHVLLCLVPAFFIAGAISVFVSQQAVIRYLGSKAKRWVAYSVAAVSGTILAVCSCTVL
                     PLFAGIYKRGAGLGPASAFLYSGPAINVLAIILTARVLGLELGIARAAGAIIFSVVIG
                     LLMALIFRKEETERQESFAELPIPEATRPLWKTAMFMAFMVVFLVFANWAAPRIPVGF
                     WAAAHRAKWVIAGIALLLTVLSARLWFSSEERSGWYGSTWGYALQVLPLLFGGVLVAG
                     FLLGRPGHEALIPGAWVAALVGGNSLFANFFASIVAAFMYFATLTEVPILQGLIGAGM
                     GKGPALALLLAGPALSLPNMLVIRSILGTKKTVVYTCLVVILSTTAGMFFGTFFA"
     gene            complement(2348..2581)
                     /locus_tag="P5541_05885"
     CDS             complement(2348..2581)
                     /locus_tag="P5541_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRV71548.1"
                     /translation="MKIQILGTGCPKCKKLAEIMESAAKELGIACEIEKVTDIMKIMD
                     FGVISTPGLAINGKVVLAGQIPSIDEAKRILSR"
     gene            complement(2626..2961)
                     /locus_tag="P5541_05890"
     CDS             complement(2626..2961)
                     /locus_tag="P5541_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV71549.1"
                     /translation="MDEIVSRKMVEIFKALAHPVRLRISRELLRREMCVCDISDLFSL
                     DRTTISKHLAVLKSSGVVKDRKEGLFVFYSLNLECLGLMIGCISNSIENELRGHPDNL
                     ESAKLETLR"
     gene            complement(3098..3592)
                     /locus_tag="P5541_05895"
     CDS             complement(3098..3592)
                     /locus_tag="P5541_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71550.1"
                     /translation="MTKIFRTTFTVLLLFSSIASAEIGISDWNSAGISLFMKKTVVVT
                     LLGSPDGNEGTMEWYDISNNEGLERVSLEYLDTDMVQAMALSFRPGSIDLDTLGSIVQ
                     GAFPGIPEVHRDEHMAVFIGRSTDTDETVYFLAMADAPDKDKGPELITMSEKANRFFL
                     DRKE"
     gene            3714..3920
                     /locus_tag="P5541_05900"
     CDS             3714..3920
                     /locus_tag="P5541_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71551.1"
                     /translation="MELQVPKCPFCGADGVVPVVYGYAPFDLLLRAEKGEVFLGGPCI
                     EEGQPQWKCNLCGRFFAVLGAPES"
     gene            3917..4390
                     /locus_tag="P5541_05905"
     CDS             3917..4390
                     /locus_tag="P5541_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRV71552.1"
                     /translation="MKRYVALAMFVLLPLAAGGLGSIATARSVAEWYPLLVKPWWNPP
                     SWVFGPVWTVLYIFMGVAAWRVWCSAGSFGSARSAMILFLLQLALNAAWSWLFFGFRM
                     PGAALAELLLLWVFIVATIRTFFVYDLVAAWMMVPYLAWVSFAGALNAAIWILNR"
     gene            4542..5036
                     /locus_tag="P5541_05910"
     CDS             4542..5036
                     /locus_tag="P5541_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRV71553.1"
                     /translation="MVIFVLSVAAYLLLAWSGGGIDLIEVGIAVILGTVMAVAARSWN
                     PERSWNLAGLNPFRWTGFVFYLFLPFLWGMIKANFDVAYRVITGKIRPGIVRIDSGLK
                     GSMAQTMLANSITLTPGTLTVDVDNSGETGIFYIHWINVTDEDPPEKKVYGSFGKWAR
                     RVAE"
     gene            5033..5293
                     /locus_tag="P5541_05915"
     CDS             5033..5293
                     /locus_tag="P5541_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRV71554.1"
                     /translation="MTVTIFSMAAFITGICALVMFGRLIAGPTVPDRVVALDSINTLV
                     IALMILLAVVYDSVVMVDVAIVYAALSFVGTMFIARHVEGGV"
     gene            5290..5667
                     /gene="mnhG"
                     /locus_tag="P5541_05920"
     CDS             5290..5667
                     /gene="mnhG"
                     /locus_tag="P5541_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRV71555.1"
                     /translation="MIVQNAPVFLLAIGVFFNVLGTIALYRFPDVYTRLHGTTKCTTF
                     GSMFTSAAVVFYAVQMHSSTGEGRFGTLAVHVVIAILALLISNPTGAHAIARAAHRSG
                     VLPRFAVVDRLAEDEKKKGGAPA"
     gene            5664..5927
                     /locus_tag="P5541_05925"
     CDS             5664..5927
                     /locus_tag="P5541_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRV71556.1"
                     /translation="MNGIAHIAVLTLIALSAFYAVWFRDLLSSVVSLAVFSLLLSLEF
                     FILQAPDVAIAEAAIGAGLSTTIYIIALRACKALRKNNGGDQV"
     gene            5924..6199
                     /locus_tag="P5541_05930"
     CDS             5924..6199
                     /locus_tag="P5541_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71557.1"
                     /translation="MKKRALFMLAILVVAGTFWGALDRIHPFGDPVRAPMDDYYLDNA
                     QQERSVNNVVTAIVFDYRGFDTLGEAAVLFTAVCSVLALFRKGREGR"
     gene            6196..6693
                     /locus_tag="P5541_05935"
     CDS             6196..6693
                     /locus_tag="P5541_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRV71558.1"
                     /translation="MNPLSIVVRTICDVFAWFMGIFGVYVIIHGHLTPGGGFQGGAIV
                     ATMIAFLLVSHGGKKLLAFARPGFYSTIETTGLLLFICAGFFGITTTFFYNFLAGQGG
                     LFGSVVPSGPNSGVINSAGTISLMNMAVGLEVIGGLSLIILYMFKGIRLFEDAEGSGE
                     TGHDR"
     gene            6683..7030
                     /locus_tag="P5541_05940"
     CDS             6683..7030
                     /locus_tag="P5541_05940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HRV71559.1"
                     /translation="MTGNAPFLVVGIIFFLGLYAILFERNLIKIAIGISLLEASANLL
                     LIVLGYRTGGSIPVFTLAGEVKTMVLPTPQALTLTAIVIGLATSALLLSLIMLLYRHY
                     GTLDVREIRRLRG"
     gene            7027..8592
                     /locus_tag="P5541_05945"
     CDS             7027..8592
                     /locus_tag="P5541_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV71560.1"
                     /translation="MNWQIHLPALALAIPLLGAFASPVAGLGGRSIRNAWMVFISIAT
                     TAICLLLLNRVLAEGVMVYVMGAESWNLTLPSGLSWPIRIILQVDAFSAFMAVVGSVA
                     ALAGAFFSLQFMDKFTGLEKFSALYFLLVTGMLGMQLTGDLFNFFVFLEIASVASFGL
                     IAFWRDRPEAVEASFKYMVISTLSAMFVLIAVGFLYGRYNAVNIAAVARMMHMGYAEK
                     IALVFLVVSLTMKCGTVPMHMWTPDAYAEAPAGVTCLLVGVSQASLYGLFRVCFSLYG
                     VSLGSTVVPWTIIVMGVLSMFIGVTMAVIQKEVKRLMAYHSVSQIGYILMALGVGLLA
                     IRDPQAMAEYGFTAMKGGIFHIINYTMYKGLLFLTAGALFYSSGSRNLNEMGGLARVM
                     PHTTFMFVIAAAAIAGIPPFNGFVSKLLIYQSTFAVYPILAVVALVTSVLTLASFVKV
                     FQTAFLGPARKKFEHATEAPGSMLIGMSILTIIIVAITLFPTWTLSHLVEPAAKALVD
                     QAGYISAVMGGGL"
     gene            8592..8930
                     /locus_tag="P5541_05950"
     CDS             8592..8930
                     /locus_tag="P5541_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRV71561.1"
                     /translation="MWPKVYSGFGYWDVYVWLIFFGISSVLVLWLRSLGRADYKEGTE
                     QDEIFYGSNEVPEDGGDMGVPASSAYWGFVKALKPYYDGLVEIHSGIVSDYVGFFVLT
                     AAVLALLVLM"
     gene            8943..9404
                     /locus_tag="P5541_05955"
     CDS             8943..9404
                     /locus_tag="P5541_05955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRV71562.1"
                     /translation="MKTERYLDILTKSIWVFHCNSGSCNGCDIEIVATITPRYDIERF
                     GMKLVGTPRHADALLVTGPVTKYMTGRLRRIYAQMPDPKVVIAIGNCACSGDVFYKSY
                     NLDGPVDNIIPVDVYIHGCPPRPEAIIEGAAKAILKLENLKEQLQKAGAAR"
     gene            9401..9952
                     /locus_tag="P5541_05960"
     CDS             9401..9952
                     /locus_tag="P5541_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRV71563.1"
                     /translation="MKPRCPEYVKESMDPKQVEDCIREKFGDAVRDVELRDHKGGAKN
                     KVAFKHLWLTIDRKRFTDLVDALMEIDFLHFHITSGDDVGNAVQLNYHFSIFRSAGYG
                     RRLGITVTVDVPKKDLVMPSLWSRIPGIEYSEREIREMLGVDFDGLPNKALIFLPEDW
                     NEEIKPWRRDETGPKPEDIRELS"
     gene            9964..11202
                     /locus_tag="P5541_05965"
     CDS             9964..11202
                     /locus_tag="P5541_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRV71564.1"
                     /translation="MAEGANKTYKVPIGPVHVGLKEPVTAWLDVDGERIVNATIRPGA
                     IHRGIEFMARDRNPIQIIYLAERICGICSFSHIVAFVRAVEDAASIEVPARAQYIRAL
                     VLELERIHSHILWAGVACYTFGYDSAFHLGMILREKVMDVLEAMTGNRVNYGTGTIGG
                     VRRDITPAVNKALRDMIDFYKNEFGVFYDVVTEDPIAKARMRNVGVLSKEDAITHCAL
                     GPTARGSGLRVDLRWSSPYEAYCDMEVTPVVPQDHTGEVYGDVFDRFLVRVLEVYQSI
                     DIIEKILAGIPSGNLMWEPKLPKLLNYLKDADGTGWSAIEAPRGDDTHSIKLTGGQEN
                     ITWWKVRAPTYANAVSWPIMFKDNELADSPLIINSIDPCISCMERMVVTDRSSGSEQT
                     VTKNDLLRLCREKTGRVMGA"
     gene            11199..12191
                     /locus_tag="P5541_05970"
     CDS             11199..12191
                     /locus_tag="P5541_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRV71565.1"
                     /translation="MILLMFKILAGVALLVFASLLSFVFEGVDRIVHARMQLRIGPPL
                     FQPFLDLLKLLGKENIVPRRAIPWAFNGAPWVSASAAMMILLYVPMGSLPPILSGSGD
                     LILILYLLGLSAVAMAVGGFASGSTYANVGAQREMTLMMSYELPLAVVIVSLAWFSYR
                     SGMPGEPFSLETFVGMPVWSVAGWTGIFGLLCLLVSLMAVVPAEVGKVPMDIAEAKTE
                     ILEGLICEYSGRNLALMKVAFSLRTLVMCAVVVSLFLPWSLGKMLQIEGPLFFLADFL
                     FFWIKVFVVQILGVTLIRTAFGRYKIWQASQFYWLHIAGLSLAGMVLLSLDVVL"
     gene            12221..12838
                     /locus_tag="P5541_05975"
     CDS             12221..12838
                     /locus_tag="P5541_05975"
                     /inference="COORDINATES: protein motif:HMM:NF006221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71566.1"
                     /translation="MIQILRQWSEDPATNPFPVAHMPDNLTEALKAAAEGTIQLNDPV
                     KTPEGFRGRIGYDRNACIGCKMCIKVCPANAIESLADEKKVIFHMDRCCFCAQCTEIC
                     PVSCIWMTHEFAFSSYDRKAEIVTDSGSKPVSQVEGKSSKKKDQGTKYEIDPEKCIGC
                     TKCARTCPVQAISGKVKEPHLIDDEKCVGCGACAEACPVKAIHVK"
     gene            12914..13957
                     /locus_tag="P5541_05980"
     CDS             12914..13957
                     /locus_tag="P5541_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRV71567.1"
                     /translation="MGKVLVIFTGGTIASGFLGDRKGPDPSVSRTLQGYVSSFFEKKG
                     VEVVCLEPWGIPGLDSSNLDPGHWIEMARIIERSVSEGTDGVLILHGTDTMAHSAAWL
                     SLCFSGVGIPIILTGSQLTLDYTPEDVTVNLRGAAQVVCSDLTGVWIYCNWKLVQGNR
                     AHKARAQHPDAFIPIGGLPLYFSPEWARDGEITDLPHPRGRMPYSLKKILGTSPDEAR
                     KAGKSVRWLLCAPGTDPVPSGNEKIIGLLGFGAGNAPTSILEKIASFYPDKEKPQIIA
                     CSQAEGDVKDPAAYHDVGIAGLSSRGFDVWGQMDYPVEFIHALCCYSLMASPQDPGKI
                     LGKFLKKYQVRAL"
     gene            complement(13968..15284)
                     /gene="gltX"
                     /locus_tag="P5541_05985"
     CDS             complement(13968..15284)
                     /gene="gltX"
                     /locus_tag="P5541_05985"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRV71568.1"
                     /translation="MNEKIRVRFAPSPTGALHIGGAHTALFNWLWARRNGGCFVLRIE
                     DTDLARSTAEHEQTILQGMRWLGIEWDEGPDTGGEHGPYRQSERIAIYREHAERLLIS
                     GQAYRDGEAIIFSVPHGRTVAFDDAVYGHIEMASETLKDIVLIKSDGFPTYNFAVVVD
                     DHTMDISHVIRGEDHISNTPKQILLYQALGWNIPVFAHLPMILGKDKKKLSKRHGATS
                     VYEYREMGYHPDAIFNYFALLGWNPGDDREIFTREEAVGMFDLSRVTKKAAVFDMDKL
                     NYINQIHISRMPPVERARHVRPFWEEAKLHPESKSDEWLARALDLLEGRGQTFKELAE
                     YTDYLLSFEPVKNRYDASELNEEQRETLERFFAAMFELPEWNPEILEVKAKTWCEENS
                     TKLKDVAMPLRWAITGRKVSPGIFHIVVFLGRDEVEKRLKYYGLLQ"
     gene            15378..17921
                     /locus_tag="P5541_05990"
     CDS             15378..17921
                     /locus_tag="P5541_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRV71569.1"
                     /translation="MSGKTLLLVDGHGLAFRAFYALPELNAPDGTPTNAILGFANMLV
                     KTFEEVRPELAAVVFDAPGPTFRHEAYEAYKEGRQPTPEEFKRQLPFIKDFSGRMGLR
                     VVERPGIEADDVIASTARSAAEQGIRVVILTADKDIFQVLAPGLSVMRPVRGVSEFRV
                     YDEKIFEEEWGFPPGAMADYLAMVGDSVDNIPGIRGVGDKTARALLASYGSLEDIYEH
                     LEDLKPALRKKMKEGHETAFSSRELTRLRFDGALETGELEIGPLERGPLREMFGRFAM
                     KKLAGRILGDEKSEEEQDPLVVAGESSMLQEWGSIDELLQEEELAFSWSGRGNYPAGF
                     SIGNICLCSKDGRYWTEEATPDLLEKLSYWAKTRYVLTSGYKEICAASPGLFPDPGKI
                     WDSVLAHYVLHPELPPDPVPGPSFEDCSKLWRLRDEQEPLIRSMSLDKVMRDIDTPLC
                     PVLAAMERHGIRVERNILGDLEEELDLKSGEISAEIDSMAGSHINLNSPKQVAWLLFE
                     KMGFTPVKKNKTGYSTDVSVLEELAEISSSKATVPRMMLEYREITKIITGFIQPLLKG
                     VDPVTGCVHTTLEHAFTGTGRLSSRDPNLQNLPSFGNLAGRLRSALRPRNKNNIFVAG
                     DYSQVELRILAHICGEERLKDAFAQGRDIHSETALWIFGEGNGSVDPEQRRMAKVVNF
                     GLLYGMSAHGLSARMGIGREEAASVIERYFKAFPKVRDYMENSAREARERGYTLTLFG
                     RRRPLNEVSTIEGRGGGALGRVSVNTPLQGSAADIARIAMIRYSHLVEESGLEAPLVL
                     QVHDSLVCECPRESVERVSVIMREAMEGSAVLSVPLEVHIKTGETFEEM"
     gene            18027..19091
                     /locus_tag="P5541_05995"
     CDS             18027..19091
                     /locus_tag="P5541_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRV71570.1"
                     /translation="MKALRTKTRAIMIIVVVVFVLSIFTMYLTGSSRSSRKSPEGDFP
                     VASVGGQNVMASQIVAGVQNFAEQSGQTDFSPESIVEMRKNVLNSIAMQHMLQKEASR
                     LKIKAPEDEIDMAVRRIENQFPTKEAFQQYMQNMGIRMKDLREQIGMQLAQRMVLDAG
                     AGNVEVSEEETREFYDQTRELFFRQPEGFNVMFARFVSMEVAEKARRELLNGGSWDDV
                     IKKYEEETIDHTLSGEPAFVTAKEFSEGSLKALNATLPGKVSEPLELSKTDVVILVKE
                     KKLAERVVPYEEASADVRSMIAEQKKQVKQNEYLETLLAGADIRMLDKGFFEVPAEPE
                     DDVSEGDTEGTEEIEDPKEE"
BASE COUNT         4356 a         4706 c         5281 g         4865 t
ORIGIN      
        1 ggtcttccag tggatggagg atgcggggcc catgccggaa ggtgactacc tcttcatggc
       61 cgagggtgtc ggccgcctca ggttcagtct attgtagacc cttcacgatt gcaggtatca
      121 agaccggagc atcttccccc cacacatcgg gcaaactcgg gctgaaaggt gacatggtct
      181 atgccgaact cactggaagc tatctcactg aggttcccga tgacaatatc agtatcgggc
      241 cgtgggtcgc tgcacagaga aacatgtgcc tcaaggaaga tgtcagtgcc gtcggtggtc
      301 cagaggtgta cgtggtgaac gtcaagcaca tccgggcttt cctcgaggcg gtctatcagc
      361 tttcgggcgt taatattacc cggggttccc tgcatgagta tgtgtatggt tgtcttgagg
      421 agtggccagg attcacggag aacgtaaagt gagaccagca gggtcagcat cggatccagc
      481 cagaaccagc cgtatgccat catgaggagt gctgcggcga taacccctac ggaggacagt
      541 gtgtccataa ctatgtgaag ataggctgcc ctgatattca ggctcttttt tgaatcgctg
      601 tgcaggagcc atgctgtaac gaggtttcca agcaacccga cgattgcaac gacaagcatt
      661 atcctaccga cgacgggctc ggggtgcagg aactttttga tcgattcaac cataagaaaa
      721 gcgccgatgc ccatcaaggc tacggtattt acgagggacg ccagtatctc cgcccgtttg
      781 aaaccgaagg aatggttcgg agtcctgtcc atgcgggata tccgccttgc cagccagctg
      841 accccaagtg aggatgcatc cgaaaagttg tggacggcgt ctgagagtag tgccaggctg
      901 ttcgagagaa ttcctcccac tatctctgca atggttatgc ctatgttcag tgccagggat
      961 atggccagcc tcctgtcgga ggtatcggat acatcgtgat gatgatgtcc ggaatgatcc
     1021 tgctcgttct cctgtccgtg attaatatgt tctgtccggt gtttggttgt gtcctttacc
     1081 atatggcagg gctcccttcg ctaagcgaaa aatgttccaa aaaacatgcc tgcagttgtg
     1141 gacagaatta ccacgagaca ggtgtagaca accgtttttt tcgtgccaag aatgctgcgg
     1201 attacaagca tgttcggcag tgaaagtgct ggcccggcga gaagaagcgc cagggccggg
     1261 ccctttccca ttcccgcccc gataagaccc tgcaggatcg ggacttccgt cagagtcgcg
     1321 aaatacatga aagccgcgac gatggaggca aaaaagtttg caaaaagaga gtttcctccc
     1381 accagggccg cgacccatgc ccccgggatc agagcctcgt gaccgggtcg gcccagcagg
     1441 aatccagcca ccaggacacc cccgaagagc aggggcaata cctgcagggc atatccccag
     1501 gttgagccgt accatccgga gcgttcttca ctcgaaaacc ataacctggc tgagaggacc
     1561 gtcaggagca gtgcgatgcc ggcgatcacc cactttgcac ggtgggctgc cgcccagaat
     1621 ccgacaggta tcctgggggc tgcccagttg gcgaagacca ggaatacgac catgaaggcc
     1681 atgaacatgg ctgtcttcca tagggggcgc gttgcttcag ggatcggtaa ctcggcgaaa
     1741 gattcctgtc tttcggtctc ctccttgcgg aagataaggg ccatcagaag ccctatcaca
     1801 acgctgaata ttatcgcccc tgcagccctg gcgattccca gttccaggcc gagaaccctg
     1861 gcggtcagga ttatggcaag tacgtttatt gccgggcccg agtataggaa cgccgaagcc
     1921 gggcccagtc ctgccccgcg cttgtagatc cccgcgaaaa gaggcagaac ggtgcatgag
     1981 cagaccgcaa gtatcgttcc agataccgcc gcgacagagt aggcgaccca tcttttggct
     2041 tttgaaccaa ggtagcgtat gaccgcttgc tgggaaacga acacgcttat ggcccccgcg
     2101 atgaagaagg caggaacaag gcagagcagc acatgttccc tggcatactc gtgcatcatt
     2161 gccaaagctt ctatcgcggc ccggtgcagt cttgtgcttt cgatgggcat gaagtagaag
     2221 gcaagaaaag ccccggcaat gtaaagaaat tttttcagat cggaattcat tttatcgccc
     2281 ctttaaagtg gcaaaaatcc cacataaaat atctttaaaa aacccgcaaa aatgcgggtt
     2341 tttggattta cctggaaagg atgcgtttag cctcgtctat ggagggtatc tgtcccgcca
     2401 gaacgacctt gccgtttatt gccagaccgg gagtggatat cactccgaaa tccatgatct
     2461 tcatgatgtc cgttactttt tcgatctcgc aggctatccc cagttctttt gccgcgcttt
     2521 ccattatctc ggccagtttc ttgcattttg gacatcctgt gccaagtatc tggatcttca
     2581 tctgccgttc tcctttctgt taatcgtgtt attgttcaga tcagtttaac ggagggtttc
     2641 aagtttcgcc gactccaggt tgtccgggtg gcccctcagt tcgttctcta tggagtttga
     2701 aatacatcct atcatcaatc ccaggcattc aaggttcagg gagtagaaga cgaagagtcc
     2761 ctctttcctg tcctttacta cgccggagga tttaaggacc gcgaggtgtt tgctgattgt
     2821 cgtcctgtcc aggctgaaaa ggtcggagat atcgcaaacg cacatctccc tacggagcag
     2881 ttcccgggat atccggagcc tcacaggatg agcaagtgcc ttgaaaatct caaccatttt
     2941 cctggagacg atttcgtcca ccctggcacc tcttctccat aaaacgatat gtggtaatat
     3001 aaccacataa ggggcttatg tcaaccccgt tcaaaaggga tttgtgggta ggttcagggt
     3061 ggtattcccg ggaaaaggtg gatggatcat gttgccgtta ttccttcctg tcaaggaaaa
     3121 aacggtttgc cttttccgac atggtaatga gttccggacc tttgtccttg tcgggggcgt
     3181 ctgccatggc aaggaaatag acagtttcat cggtatccgt tgatcttccg atgaagacag
     3241 ccatatgttc atcccggtga acttccggaa tcccggggaa cgcgccctgc acgatagatc
     3301 cgagagtatc aagatcgatc gatccgggtc tgaaggatag cgccattgcc tggaccatgt
     3361 ccgtgtccag atactcaaga gagactcttt cgaggccttc attatttgaa atatcatacc
     3421 attccatggt tccctcgttc ccgtcgggtg agcccaggag agtcacgacc actgtttttt
     3481 tcataaaaag ggatatcccg gccgagttcc agtcggagat accgatttcc gcggaagcta
     3541 ttgacgaaaa taacagcaaa actgtgaaag tggtacggaa tatttttgtc attctgtttc
     3601 atttcctttc ctgaaagagt tacaataccc catgtgaatt gtttcaggcc actgacacag
     3661 ggtaacatgc atgacagttt cctgaaagag taaaatccgg aggtatccga aagatggaac
     3721 tgcaagtccc aaagtgccca ttttgcgggg ccgatggtgt tgtcccggtt gtgtacggtt
     3781 atgctccttt cgatctgctc ctgagggccg aaaaaggaga ggtgtttctg ggaggtccat
     3841 gcatagagga aggacagccg caatggaagt gtaatttatg cggccgtttt tttgctgttt
     3901 tgggggcgcc cgaaagttga aaagatacgt ggcacttgca atgtttgttc ttttgccgct
     3961 tgcggcgggg ggccttggtt cgatagcgac tgcccgttca gtggcagagt ggtacccgct
     4021 tctggtcaag ccctggtgga accctccttc atgggtcttt ggccctgtct ggacggtact
     4081 ttatatcttt atgggcgtag cggcgtggag ggtatggtgc tctgcgggca gtttcggttc
     4141 agcgcgttcc gccatgatcc tctttttatt gcaactggca ctgaatgcgg cctggtcatg
     4201 gctgttcttc ggtttcagga tgcccggagc agctttggct gaactcctgc tgctatgggt
     4261 ttttattgtg gctacaataa ggactttttt cgtttacgac cttgtcgcgg catggatgat
     4321 ggttccatat cttgcctggg tatcctttgc cggagctctc aacgctgcca tatggattct
     4381 gaacaggtag tttcatgtat tgttttctaa tgtataatgt atatccggag tcttgtcaga
     4441 ctggcatttt ccccctttcc tctgtatctt ttcgtacatg gcgggggttt taaaaacctg
     4501 atggatcaag tttttgaaaa cggaattggg aggtgaggcg tgtggttatt tttgtcctct
     4561 ccgtcgctgc ttatcttttg ctggcgtgga gtggaggcgg tattgatctt atcgaggttg
     4621 gcattgccgt gatccttgga acggtaatgg ctgtggccgc caggtcctgg aatcctgaaa
     4681 ggtcctggaa tcttgcaggt ttaaacccgt ttcgctggac aggatttgtt ttttatcttt
     4741 ttctgccttt tctctgggga atgatcaagg caaattttga cgttgcctac agggtcatta
     4801 cagggaaaat acgcccgggt atagtgagaa ttgactcggg tctcaagggc agcatggccc
     4861 agaccatgct cgccaactcc attaccttga cgccgggaac gctcaccgtg gatgtggata
     4921 attccggaga gactggcata ttctatatcc actggattaa cgtcaccgac gaagatcccc
     4981 ccgagaaaaa ggtttacggc tctttcggga aatgggcgag gagggttgcc gaatgacagt
     5041 taccattttc tccatggccg ctttcattac gggtatatgt gcgttggtga tgttcggaag
     5101 gcttatcgcc ggaccaaccg tacctgacag ggttgttgct cttgattcaa tcaatactct
     5161 agtgatcgca ttaatgatac tccttgcggt tgtgtatgac tcggttgtta tggttgacgt
     5221 tgctattgtc tacgccgccc tttcctttgt gggaacaatg tttattgcac gccacgtaga
     5281 agggggtgtg tgatcgtgca gaatgccccg gtttttcttc tagccatcgg cgtgtttttc
     5341 aatgttctcg gcacgatagc actctatcgt ttccctgatg tttataccag gcttcacggg
     5401 acgaccaaat gtactacttt cgggtcaatg ttcacctcgg ctgcggtggt tttctatgcg
     5461 gtccagatgc attcctctac cggtgaaggg cggtttggaa cccttgcggt tcacgtggtt
     5521 atagccattc tggctctttt gatatccaac ccgacaggag cccacgccat agccagggca
     5581 gcacaccgaa gcggtgttct tcccaggttt gccgttgtgg acagacttgc cgaagacgag
     5641 aaaaagaagg gcggtgctcc cgcatgaacg gtatcgcgca catcgctgtt cttactctga
     5701 tagcactttc cgccttttat gcggtctggt tccgggatct tctttcctcg gtagtatcgc
     5761 tggcggtttt cagccttctg ctttctctgg agttcttcat actgcaggct cctgatgtag
     5821 cgatcgcgga agcagcgata ggggctggcc tgagtacgac catctacata attgctctgc
     5881 gggcctgcaa ggctctcagg aaaaacaacg ggggtgacca ggtatgaaaa aacgagctct
     5941 cttcatgctg gcgatccttg tggtcgcagg cacgttctgg ggagccctgg acaggatcca
     6001 ccccttcggc gatcctgttc gcgccccgat ggacgactac tatctggata acgcccagca
     6061 ggaacgttct gtcaataacg tcgtaacggc tatagttttt gactacaggg gttttgatac
     6121 cctgggagag gcagctgttc tcttcacggc ggtctgttcc gtactggcgc tcttcaggaa
     6181 ggggagggaa ggacgatgaa tcctctttcg atcgtcgtaa ggaccatatg cgatgtcttt
     6241 gcctggttta tgggcatatt tggagtttat gtaataatcc acggtcacct tacccctgga
     6301 ggggggtttc aggggggagc gatcgtcgcc acgatgatag ccttcctgct tgtatcccat
     6361 ggaggcaaaa aactgctggc ttttgccagg cccggtttct attccacgat agaaaccacg
     6421 ggtcttcttc tctttatctg cgccggtttt tttggtatta ccaccacctt tttctacaac
     6481 ttccttgcag gccagggggg acttttcggt tctgtcgtcc cctccggtcc caacagtggg
     6541 gttattaaca gcgccggaac aatatccctg atgaacatgg ccgtcggtct tgaggtcatc
     6601 ggaggtcttt ccttgataat cctctacatg ttcaagggaa ttcgcctgtt cgaagacgct
     6661 gaaggaagcg gggagacggg ccatgacagg taatgcgcca tttctcgttg tcgggatcat
     6721 tttctttctg ggcctttacg cgatcctttt cgagaggaac cttatcaaga tcgctatagg
     6781 catctctctt cttgaagcgt cggccaacct gttgcttatt gttcttggtt accggaccgg
     6841 aggaagtatc ccggtgttca cccttgccgg agaggttaaa accatggttc ttccgactcc
     6901 gcaggcgctc acactgacgg cgatagttat aggcctggct acatctgcgc tgctcctttc
     6961 gctgataatg cttctttacc gtcattacgg gacacttgat gtccgcgaga tcaggaggct
     7021 gagaggatga actggcaaat tcaccttccc gcgcttgctc tggccatccc tcttcttgga
     7081 gcttttgcct cgcccgtcgc tggtcttggc ggacgatcca tccggaatgc gtggatggta
     7141 ttcatatcca ttgccacaac ggcgatctgc ctgcttctcc ttaacagggt tctcgccgaa
     7201 ggtgtaatgg tttacgtcat gggggcagag agctggaatc tgactcttcc ctcgggcctg
     7261 agctggccca taaggatcat tctccaggtc gatgctttca gtgcgttcat ggctgttgtc
     7321 ggttccgtgg ccgccctggc tggagccttc ttttcacttc agttcatgga caaattcact
     7381 ggtcttgaaa aattttctgc cctttatttc ctgcttgtaa cgggaatgct gggaatgcag
     7441 cttactggag accttttcaa tttctttgtg tttctcgaga tagcctcggt ggcttccttc
     7501 gggcttattg cattctggag agacaggccg gaggcagttg aagcatcatt caagtacatg
     7561 gtcatatcga ccctttccgc catgttcgtg cttatagccg ttggattcct ttacggccgg
     7621 tacaatgcag tcaacatcgc tgcagtagcg agaatgatgc acatgggtta tgccgaaaag
     7681 atcgctctgg tgtttcttgt tgtttccctg actatgaaat gcgggaccgt tccgatgcat
     7741 atgtggactc ccgatgccta cgccgaagcg cccgcgggag ttacctgcct tctcgtcggg
     7801 gtcagccagg cttcacttta cggtctcttc cgggtctgct tctctctata tggtgtaagc
     7861 ctcgggagca cagttgttcc gtggacgatc atagtgatgg gcgtcctttc catgttcata
     7921 ggtgtgacca tggccgttat ccagaaagag gtaaagcgtc tcatggccta tcattcggtc
     7981 tcgcagatag gttacatcct catggccctt ggagtaggtc tcctggcgat cagggatccg
     8041 caggccatgg ctgaatacgg tttcaccgcg atgaagggtg gtattttcca cataataaac
     8101 tacacaatgt acaaggggct gcttttcctc accgccggtg ctcttttcta ttcatccggt
     8161 tcgagaaatc tcaacgaaat gggaggcctt gcgcgggtga tgccccatac gacattcatg
     8221 ttcgtcatag cagccgcggc tatcgcaggg atacctccgt tcaacggttt cgtttccaaa
     8281 ctgttgatct accagtcaac atttgcggtt taccccatcc tggcggtggt ggctctcgtg
     8341 acctctgttc ttacccttgc ctcttttgtc aaggtttttc agacggcctt cctgggtccc
     8401 gcgagaaaaa aattcgaaca tgctaccgaa gccccgggtt cgatgcttat cgggatgtcc
     8461 attcttacga ttattattgt ggccattacg cttttcccga cctggaccct ttctcacctt
     8521 gttgaacctg cggccaaggc tctggtagac caggccggct atatttccgc ggtcatggga
     8581 ggtggtctct aatgtggccc aaagtctatt ccggtttcgg gtactgggat gtctatgtct
     8641 ggcttatctt tttcggaata tcttcggtgc ttgtcctgtg gctgaggtct cttgggagag
     8701 cggactacaa ggaagggacc gaacaggacg agatctttta cggaagcaat gaggttccgg
     8761 aagacggcgg tgatatgggt gttccggcgt cgtctgccta ctggggcttt gtgaaggccc
     8821 tcaagcctta ttatgacggt cttgtcgaaa tccacagcgg cattgtaagt gactacgtgg
     8881 gctttttcgt cctcactgcg gcagtccttg cgttgctcgt cctgatgtag gggggtgtga
     8941 cgatgaaaac agaacgctat ctcgacatcc ttaccaagtc gatatgggtt ttccactgca
     9001 acagcggctc ctgcaacggc tgcgacatcg agatcgttgc gaccattacg ccaaggtacg
     9061 atatagaacg cttcgggatg aagcttgtcg gcacaccaag gcatgcggat gcgctccttg
     9121 tgaccggtcc agtcacaaag tacatgacgg gcaggttgcg ccggatctat gcccagatgc
     9181 ctgaccccaa agttgtcatt gcaattggca actgtgcatg ttctggtgat gttttttaca
     9241 agtcttacaa tcttgacggt cccgttgaca acataatacc cgtcgatgtc tacattcatg
     9301 gatgtccccc gagaccggag gccataattg aaggtgccgc caaggcgata ctcaaacttg
     9361 agaacctgaa agaacagctt cagaaggcag gtgccgcaag atgaaaccgc gttgcccgga
     9421 atatgtaaaa gaaagcatgg atccaaagca ggtagaggat tgtattcgag agaaatttgg
     9481 tgatgcggtc cgtgatgtgg aactccgcga tcataagggt ggcgccaaaa acaaggttgc
     9541 tttcaagcac ctctggctta ccatcgacag gaagcgtttt acggatctcg tcgatgccct
     9601 tatggaaata gactttctcc actttcacat cacttcgggg gatgatgttg gcaacgccgt
     9661 ccagctcaac tatcactttt ctattttcag gagcgctggt tacggcaggc gactcgggat
     9721 cacagtcacc gtcgacgtgc caaagaagga tctcgtaatg ccttctctct ggagcaggat
     9781 tccaggaatc gaatacagcg agagggagat ccgggagatg ctgggtgttg acttcgatgg
     9841 cctgcccaac aaggccctga tcttccttcc tgaggattgg aatgaagaga tcaaaccctg
     9901 gcgtcgtgat gagaccggtc ccaagccgga agatatcagg gaactttcct aggaggtgcc
     9961 gaaatggctg aaggagcgaa caagacctac aaagtgccaa tcggcccagt ccacgttggt
    10021 ctgaaggaac ctgtgactgc atggcttgat gttgatggtg agcgtattgt caacgcaacg
    10081 atccgcccgg gagccattca tcgcggtatc gagttcatgg cccgcgacag gaaccctatc
    10141 cagataatct atctggcaga acggatctgc ggcatatgct ctttttcgca tatagtcgcc
    10201 tttgtgaggg cagttgagga cgccgccagc atcgaggtcc ctgcccgtgc gcagtatatt
    10261 agggcgctgg ttctggagct tgaaaggatc cattcccata ttctgtgggc cggcgttgcc
    10321 tgctacactt ttggttacga ttccgcgttc cacctgggga tgatcctcag ggaaaaggtg
    10381 atggatgttc tcgaggctat gaccggaaac cgggtaaact acggtacagg tacgatcggc
    10441 ggggttcgga gagacattac gccggctgtg aacaaggcac taagggatat gatcgatttt
    10501 tacaagaacg agttcggagt cttctatgat gtggtaactg aggatccaat agccaaggcc
    10561 agaatgagga acgtgggtgt gctttcaaaa gaggatgcaa taactcactg cgcccttggt
    10621 ccgaccgcca gaggaagcgg cctgagggtt gatctcaggt ggtcttcccc gtacgaggcc
    10681 tattgtgaca tggaggtaac ccctgttgtc ccacaggatc atacggggga ggtttacggg
    10741 gacgtgttcg acagattcct ggtcagagtt ctggaggtct accagtcaat agacattata
    10801 gagaagatat tggccgggat tccatcaggc aaccttatgt gggaaccaaa actccccaag
    10861 ttgctcaatt accttaaaga tgccgatgga acagggtgga gtgcaataga ggctccccgg
    10921 ggagacgaca cgcactccat aaaacttaca gggggccagg agaatattac ctggtggaag
    10981 gttcgtgccc cgacatatgc gaatgccgtc tcgtggccga tcatgttcaa agacaatgaa
    11041 cttgccgatt cgcccctgat cataaacagt attgacccgt gtatttcatg tatggaacgc
    11101 atggtagtga cggatcgctc ctccggttcg gagcagaccg tcaccaaaaa cgatcttctc
    11161 aggctttgcc gcgagaaaac ggggagggtg atgggagcat gattcttctt atgttcaaga
    11221 tcctcgcagg ggtggcgctc ctcgtgtttg cctctcttct ttcattcgtg ttcgaagggg
    11281 tagacaggat agtccatgcc aggatgcagc tgaggatcgg gccgcccctg tttcagccct
    11341 tcctggacct cctcaagctt cttggcaagg agaacatagt gccccgcagg gctattccat
    11401 gggctttcaa cggtgccccg tgggtttctg cttctgccgc gatgatgatc cttctttatg
    11461 tgccgatggg ttccctgcct ccgattctaa gtggaagcgg ggatctcatc cttattcttt
    11521 atcttctggg gctttccgca gttgccatgg cagtgggggg ttttgccagc ggatcgacct
    11581 atgcgaacgt tggagcccag cgcgagatga ctctcatgat gagttacgaa ctgcccctcg
    11641 ctgttgtgat agtctccctg gcgtggttct cctatcgttc gggtatgcct ggagagcctt
    11701 tcagccttga aaccttcgtc ggaatgcccg tctggagtgt tgccggctgg acaggaatat
    11761 tcggtcttct ctgtcttctg gtgtcactta tggcagtggt ccccgccgaa gtgggaaagg
    11821 ttccgatgga catagccgag gccaagaccg agatactgga agggctcata tgcgaatatt
    11881 caggccgcaa ccttgccctg atgaaagttg cattttctct tcgcacgctt gtaatgtgcg
    11941 cagttgttgt ttcactcttt ctcccgtggt cgctcgggaa aatgcttcag atagaggggc
    12001 cgctcttctt cctggccgat ttccttttct tctggatcaa ggtttttgtt gtacagatac
    12061 ttggagtaac ccttatccgg acagctttcg gccgatacaa aatatggcag gcatcgcagt
    12121 tttactggct tcacatagcg ggcctttccc ttgccgggat ggtccttctt tcgctggacg
    12181 tagtccttta aagggggggt tgtaaatgct caacagaatg atgatacaga tattgagaca
    12241 gtggagtgag gatcccgcca ccaacccctt tccggtggcg cacatgccgg ataatctcac
    12301 cgaggccctc aaggccgcgg ccgaggggac aattcaactt aatgatccgg tgaaaactcc
    12361 tgagggattt cggggaagga tcgggtacga cagaaatgcc tgcataggat gcaagatgtg
    12421 cataaaggtc tgtcctgcca acgccatcga gtcccttgct gacgaaaaga aggtgatttt
    12481 ccacatggat cggtgctgtt tctgtgccca gtgcacggag atctgccccg tttcgtgtat
    12541 atggatgacc catgaatttg ctttttcttc atacgatcgc aaggccgaga tcgtgaccga
    12601 ttctggaagc aagccggtct ctcaggtgga gggaaaatca tccaaaaaga aggaccaggg
    12661 aacaaaatat gagatcgacc ccgagaagtg tatcgggtgc accaaatgcg caagaacctg
    12721 cccggtccag gcaatatcgg gtaaggttaa ggagcctcac ttgatcgatg atgaaaagtg
    12781 tgtcggatgc ggggcctgcg ccgaagcgtg cccagtcaag gctattcacg taaaatagac
    12841 tacggcagaa gctgatgacc ttccggcggg agcctttagc ggaggccccc gccttttttt
    12901 tcggaggtgg cgaatgggga aagtgcttgt catctttacc ggaggtacaa tcgccagtgg
    12961 ttttctggga gacagaaagg gccctgatcc gtcggtttcg cggactcttc agggatatgt
    13021 ctccagcttt ttcgaaaaga aaggagtgga agttgtctgc ctggagccat ggggtatccc
    13081 tggtcttgac agttcgaacc ttgacccggg gcactggatc gagatggccc gtattatcga
    13141 gagatctgta agtgaaggaa ccgacggggt tctgatcctt cacgggacgg ataccatggc
    13201 tcactccgcc gcatggttga gcctttgctt ttccggtgtc gggattccta taatcctgac
    13261 ggggagccag ttgacacttg actatacgcc ggaggacgtt acggtcaatc tcagaggtgc
    13321 ggcgcaggtg gtctgttctg atctgacagg agtatggata tactgcaact ggaaacttgt
    13381 ccaggggaac agagcccaca aggccagggc ccaacaccct gacgcattca tacccatagg
    13441 aggtcttccc ctttatttca gcccggaatg ggctagagac ggggagatca ccgatctgcc
    13501 tcatccaaga ggaagaatgc catattcact gaaaaagatc cttggcacct cccccgatga
    13561 agcaaggaag gcggggaaat ccgtaaggtg gttgctgtgt gctccgggaa ctgatcccgt
    13621 tccgtctggc aacgagaaaa taattggcct gcttggattc ggggcaggca atgccccgac
    13681 atcaatactt gaaaaaattg cctctttcta tcccgacaag gaaaagccac agatcattgc
    13741 ctgcagtcag gccgaaggag acgtaaagga tccggcagcc tatcatgatg taggcatagc
    13801 gggtctttca tctcgcggtt tcgatgtctg gggacaaatg gattaccctg ttgaattcat
    13861 ccatgcgctt tgctgctact cgcttatggc ctcgccccag gatcccggaa agatccttgg
    13921 taaattttta aagaaatatc aagtaagggc gctctgagag agcgccctta ctgtaaaagg
    13981 ccgtaatatt tcaatctttt ttcgacttcg tccctaccga ggaacactac gatgtggaag
    14041 atcccggggc ttacctttcg cccggttatt gcccatctga gaggcattgc tacatccttc
    14101 agttttgtgg aattttcctc acaccacgtc tttgccttta cctcaagtat ctcggggttc
    14161 cattcaggta actcgaacat ggctgcaaag aacctttcaa gggtctctct ttgttcttcg
    14221 ttcagttccg aggcgtcata gcggtttttt acgggctcga aggagagaag atagtctgtg
    14281 tattctgcca gttccttgaa ggtctgacct cttccctcaa gaagatccag agcccgggcg
    14341 agccattcgt cacttttcga ctccgggtga agctttgcct cttcccagaa tggacgtacg
    14401 tgcctggccc gttcaaccgg cggcattctg gaaatatgga tctggttaat ataattgagt
    14461 ttatccatgt cgaagaccgc ggcttttttt gttacccttg aaagatcgaa catgccgacg
    14521 gcttcttcac gggtgaatat ctccctgtcg tcaccgggat tccatccaag aagcgcaaaa
    14581 tagttaaaga tcgcgtccgg gtgatatccc atttccctgt attcgtaaac gctggtagct
    14641 ccgtggcgtt tggaaagttt tttcttgtcc ttgccaagta tcatgggtag atgggcaaat
    14701 acaggaatgt tccagccaag tgcctggtac aggaggatct gcttcggagt gttagagata
    14761 tgatcttcgc cgcgtattac atgagagata tccattgtgt ggtcgtccac caccacggca
    14821 aaattgtagg tgggaaatcc atcgcttttt atcaggacta tgtccttcag ggtttcgctg
    14881 gccatttcga tatgaccgta aacggcgtcg tcgaatgcaa ctgtcctgcc atggggaact
    14941 gaaaaaataa tggcttctcc gtcccgatac gcctgacccg agatcagaag cctttcggca
    15001 tgctcccggt atattgctat cctttccgac tgcctgtagg ggccgtgttc cccacctgta
    15061 tcgggtcctt catcccattc gatacccagc catctcatgc cctggagaat ggtctgctcg
    15121 tgttcggccg tggatcttgc gagatcggta tcttctattc gaaggacgaa gcagccgccg
    15181 tttcttcggg cccatagcca gttgaaaagg gctgtatgag ctcctccgat atgcagcgcc
    15241 cccgtagggc tgggtgcgaa acgtacgcgg attttttcgt tcattattgt tcatccttcc
    15301 atgatggtat gctagtcgat attgaaaacg cacagtgcag tatacctcaa aaatggcggt
    15361 caaaggagat gtagacattg tcggggaaaa cccttcttct ggtggatggc catggccttg
    15421 ccttccgtgc tttttatgct cttccggaac tgaacgcgcc tgacggaact cccacgaacg
    15481 cgatcctggg ttttgcgaac atgcttgtca aaacctttga agaagtacgg cccgaactgg
    15541 ccgcggttgt ttttgatgcc cccggaccca ctttcaggca cgaagcctat gaagcctaca
    15601 aggaaggcag gcagcctaca cctgaagaat tcaaacggca acttcctttt atcaaggatt
    15661 tctctggcag gatgggcctc cgggtcgtag aaaggccagg aattgaggcc gacgatgtga
    15721 tcgcatccac agccagatcc gcggcagaac agggtatcag ggttgtaata cttaccgcag
    15781 acaaggatat ttttcaggtc cttgccccgg gactttcggt catgagaccc gtgaggggag
    15841 ttagcgagtt cagggtctac gacgaaaaga tctttgaaga ggagtggggc ttcccgcctg
    15901 gggctatggc cgattatctg gcgatggtcg gagacagcgt agacaacatt ccgggcataa
    15961 ggggagtagg ggacaaaacg gccagggcgt tgcttgccag ctacggatca ctggaagata
    16021 tatacgaaca tctcgaagac ttgaaaccgg cccttcggaa aaaaatgaaa gagggccatg
    16081 aaacggcttt cagcagcagg gaattgacaa gactaaggtt cgatggagca cttgaaacgg
    16141 gagaactgga gatcggtcca ctggaaaggg gaccactcag ggaaatgttt gggcgtttcg
    16201 cgatgaagaa actggcaggg cgtattctcg gcgatgaaaa aagcgaagaa gagcaagatc
    16261 cgctggtggt tgccggggag tcctcaatgt tgcaggagtg gggatccatt gatgagttgt
    16321 tgcaggagga agaactggct ttttcctggt cgggcagggg caactaccct gctggttttt
    16381 ccatcggaaa tatctgcctg tgctcaaagg acggaagata ctggacagaa gaggctaccc
    16441 cggatttgct ggaaaaactg tcttactggg caaagacccg ttatgttctc acaagcgggt
    16501 acaaggagat atgtgccgcg tcaccgggac ttttccctga tccagggaaa atatgggatt
    16561 ccgtattggc tcattatgtc cttcacccgg agttgccacc tgacccggtg cccgggcctt
    16621 ccttcgagga ctgttcgaaa ctctggcgtc tcagggatga acaggaaccc cttataaggt
    16681 cgatgagcct tgacaaggta atgagagata tcgatacacc cttatgcccc gttcttgcgg
    16741 ccatggaacg ccacggaata agggtcgaaa ggaatattct gggcgatctt gaggaagaac
    16801 tggacctcaa aagcggggaa atttcggcgg agatagattc tatggctgga tcacacataa
    16861 atctcaactc tccgaaacag gttgcctggc tgctcttcga aaaaatgggc tttaccccgg
    16921 tcaaaaagaa caaaaccggg tactcgacag atgtttcggt actggaagaa ctggcggaga
    16981 tctcctctag caaggcgaca gttccccgaa tgatgcttga gtacagggag ataaccaaga
    17041 taattacagg tttcatccag cctcttctta aaggtgttga ccctgtaaca ggctgtgttc
    17101 atacaacatt ggagcatgct ttcactggga ccgggcgcct cagcagcagg gacccaaacc
    17161 ttcagaacct tccctccttc ggcaatctgg cgggcaggct cagaagcgcg ttaaggcccc
    17221 gaaacaagaa caacatattt gtagccggtg attactcgca ggtggagctc aggatccttg
    17281 cgcatatttg tggagaggaa aggctcaaag atgcttttgc ccagggaagg gacattcatt
    17341 ccgaaacggc cctctggatc tttggcgagg gaaatggttc cgtagaccct gagcagagac
    17401 ggatggcaaa ggtcgtcaac ttcgggcttc tatacgggat gagcgcccat ggtctttccg
    17461 ccaggatggg aataggcaga gaagaagcgg cttcggttat agagagatac ttcaaggcct
    17521 tcccgaaggt aagggactac atggagaatt cagcccggga ggccagggaa agaggttata
    17581 cgctgaccct tttcggaaga agacgtcctc tcaatgaggt ttccaccatc gagggaaggg
    17641 gagggggggc cctcggaaga gtttccgtga atactcctct tcaagggagt gccgcggaca
    17701 ttgcgcgtat tgccatgatt cgttattcac atttggtgga ggagtcggga ctggaagcgc
    17761 ctctcgttct tcaggtccat gactcactgg tatgtgaatg tccccgggaa tctgtcgagc
    17821 gggtttccgt gataatgagg gaggccatgg aaggatcggc tgtgctctcg gttccgcttg
    17881 aagtccacat aaagacgggg gaaacatttg aagagatgtg actttttctt tgacaccggt
    17941 tcctttcaaa cgtatcattg aacacgctga aaaaaacagg ctttgtcagt ttcgcctgtg
    18001 cataaggagg aaaacagacc gtgataatga aggcactaag gacaaaaacc agggccatta
    18061 tgataattgt ggtggtcgtt ttcgtgctgt cgatattcac aatgtacctt acgggcagca
    18121 gtcggtcctc acgaaaatcc cccgaaggag atttccccgt cgcttcagtt ggcggacaga
    18181 atgttatggc ttcccagata gtggcaggag tccagaactt tgcggaacag tcgggacaga
    18241 ctgatttctc ccctgaatca atcgttgaaa tgcgcaagaa tgtactaaac agcatcgcca
    18301 tgcagcacat gcttcagaaa gaagcgtccc gactgaagat aaaggcgcct gaagatgaaa
    18361 tcgacatggc agtaaggagg atagaaaacc agttccccac gaaagaggct tttcaacagt
    18421 acatgcagaa catgggtatc aggatgaaag atctccggga gcagattggc atgcaattgg
    18481 cccagaggat ggttctggat gcaggtgccg ggaacgtgga ggtcagcgaa gaggaaaccc
    18541 gtgagttcta tgaccagaca agggagcttt tcttccgcca gcctgagggg ttcaatgtta
    18601 tgttcgcaag gttcgtatcc atggaagttg cagagaaagc ccgccgggaa ttgctgaatg
    18661 gcggttcatg ggatgatgtc ataaaaaaat atgaggaaga aaccatcgat cacaccctgt
    18721 ctggcgaacc ggcttttgtg acggcgaaag aattttctga aggaagcctc aaggccctga
    18781 acgcaaccct ccccggtaaa gtttccgagc cactggagct gtcaaagacg gatgttgtca
    18841 ttctggtcaa ggagaaaaaa cttgcggaga gggtcgttcc ctacgaagaa gcaagtgccg
    18901 atgttcgatc catgatcgcc gagcagaaaa aacaggttaa gcagaatgaa tatcttgaga
    18961 ccctccttgc tggagcggat ataaggatgc tggataaggg attttttgag gttcccgcag
    19021 aacccgaaga tgatgtctct gagggagata cggaaggaac tgaagagatc gaagatccga
    19081 aagaggaatg atggagactc tccttgacac gttcttcaag ggtgcataga atagaccgcg
    19141 catgattgct cccggtggga tggccgagcg gctgaaggca ctcgcctgct aagcgagtag
    19201 gggggctt
//
LOCUS       DAOTIQ010000037        19075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3465_length_19075_cov_8.437487, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000037 DAOTIQ010000000
VERSION     DAOTIQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 19075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19075
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3465_length_19075_cov_8.437487"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5541_06000"
     CDS             complement(<1..304)
                     /locus_tag="P5541_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71571.1"
                     /translation="MRREAEDQGGRRLASGDRKAWAKAGGGKAKGRSEGFTEGSGAGE
                     FRSRRGGAGKAHSSGWFLPALCGRPVAGGETNDFRRVAFGKDFTTHSHKTIPRPFSP"
     gene            632..1876
                     /locus_tag="P5541_06005"
     CDS             632..1876
                     /locus_tag="P5541_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRV71572.1"
                     /translation="MEKAEKQSSGLVRSVRAMWEKVPFYRSRMEDMGVCPEDIVSVED
                     LHKLPFMTKSDLRDAYPMGILACDRDDVVRVHASSGTTGKPTVVPYTRNDITTWEECM
                     GNCLTTAGVTRGDVFQVILGYGLFTGALGFHYGAEKLGAMVIPTGGGFTGRQLMLMED
                     LGTTVFTSTPSYALHLCEEVRKNGLREKLRLRLAILGGEAWTEQMRDRIENELGVIAL
                     DSYGLSEVIGPGVAMECPERAGLHPDWEHFIFEIVDPETGRVLPEGKEGELVVTSLTK
                     EAMPVIRYRTRDLTRFLPGRCPCGRDDMRLERIKGRCDDMLIIRGVNVFPSQVEAVLG
                     KIEGLSLHYRLDVFQPNGLKELNVLCEAGRELDEVAMGRMQKEVTKKLHENLGIRVGF
                     SLLSPGTLERSAGKAVRVTQVA"
     gene            1902..2057
                     /locus_tag="P5541_06010"
     CDS             1902..2057
                     /locus_tag="P5541_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71573.1"
                     /translation="MMERDMVEKREVCCGSCVNDSSLVKPIDIDVDFEGLAIAWKEVS
                     ETSGKRV"
     gene            complement(2054..3268)
                     /locus_tag="P5541_06015"
     CDS             complement(2054..3268)
                     /locus_tag="P5541_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71574.1"
                     /translation="MITPGLVETLFSAASMTRWNDHARPAQFTELAKQAHKMTIAWTI
                     ARCEEDNGKTVDWVALVEGGLFEFFQRVILTDIKPPVFHRMMANHGPKLNQWVLEKTA
                     PDLSPLSGGVFGRFRRYLEEPGFSAREKHILRAAHYLATQWEFRMIYAQNSMLWGIEE
                     TRKEIEDQVNEHLDLSGVKEILMRQGMADKGVFAFIDLCGQLRFQVRWAQIPRVPATS
                     VLGHLLIVASLSFFSALEEGASPRRLRNDFFGGLFHDLPEVLTRDIVSPVKRSVGGLE
                     GLIKEYEQEAMENRLLPLLPEKWRDEIRYLTKDEFSNRARDPLTSNIVSGIDPSDMNR
                     IYNTDDRDPVDGLLIDACDKLAAMAEASLSIRFGVRPPALVEGRRNIFDSYRKTEIGS
                     TDFGTLFGAFMD"
     gene            3359..4240
                     /locus_tag="P5541_06020"
     CDS             3359..4240
                     /locus_tag="P5541_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRV71575.1"
                     /translation="MNISGWFAEFNLEVDLIFEYLRRERNLDFSGYSPLMIERCIKNR
                     LEASGALDLTGYLLMLENNRMELDSLLDSLTIGVSEFFRDPMVFSAIETFVLPTIFRD
                     KVVSGDHSIRIWSAGCSTGEEPYSVAILSKESHFPGKLPVDIDIFGTDIDEKDLETAK
                     TGEYPMERLNNVRMGLFGKYFEPQVDHYRVKKGIRDMVCFSYHDLLSQKEQVPGISVF
                     GAFDMILCRNVLIYLDKKYQEIVLERLDRALRPGGFLVLGEAESLDAARRMRFGQICD
                     FCRVFRKNPVHRYGGGS"
     gene            4243..7875
                     /locus_tag="P5541_06025"
     CDS             4243..7875
                     /locus_tag="P5541_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV71576.1"
                     /translation="MVDRLMFRGIAKKFLFWFLVVAVVPLGISLWVITTQMIGSARQN
                     VSENLAAIRDMKVSHFRQWLDNKQVNVKVLAEDTAVRDLVEKIETGQGNREELLQKAR
                     EVLGRQLSLKPDFLDFFLVDSRTGVIFLATEPSIEGLNRSGDAYFTGPLEKNGTYIKD
                     IYRSEALGGEPTMTISTPVYCHQHGGEHITAVLVGRLDLENSLYALLRAREGLGQTGE
                     MVLVNRESIALNTLAKAPDAPLRIKITAEPARLAAQGGTGVVEEKDYAGEKVLSAYTH
                     LPELGWGFVVKMDQTEIYAPIAAMVRRILITGLAFFLLTVALALAVSRGLARPLESMA
                     KISEKIARGELDARNTDEERNDEIGDLAGSINTMAERLQYRFGLLEGLSSVTEAAVGS
                     YDREGFARAVFEVLMARIHAHMGVFYVLSGDGKTFEPSFSAGMEPKAREPFDRETLEG
                     ILGFSSLRDDVVVIRQIPDAAPFVFRGPGGVVEAREIMALPLLVSGSLEGLICLGSLT
                     SFQWEDEELVRECRHSLSTGLDRILASERMQEMTAQIKARNEELVSQTEELTRQSEEL
                     QEQNVELEHQRKMIEEANRLKSEFLSNMSHELRTPLNSILALSRVLQRRGGQKEEDEA
                     KYLDVIERNGRQLLDLINDILDLSKIESGRVEIRPSLFSLSDTVISLLETLRPVAIEK
                     EIFLEADIPKDLPLVKTDDSRLRQILRNLLDNAVKFTPEGGVAISVRNEGGEFVIRVK
                     DTGIGIPKEELPFIFDEFRQVDGSSSRQFEGTGLGLAIAKKSAWLLAGRLTAESREGE
                     GSTFTLSIPMELDSFSQKAIEGPFMAGGEGKKVIVVEDDVSVASTISSLLSRIGYEAI
                     VARSGHDALRLVRQVNPVAMTLDIIMPEMDGWEVLQKIKGDPETSALPVVIISVCDEV
                     DTAKALGADGYLCKPVIERDLSAEIQRVCGKDSGRVIVAGEASGRRTILMVEDNDIAA
                     RQVKHLIEEEGIAFVEIAPGGQQALEYMKENTPDAIILDLMMPGVDGFSVLDQLRGRE
                     TTAGIPVLILTAKDLTREDLSRLRSNNVQQLVQKGDVDREEILRKIRLLFGETVATNG
                     DIRPDVLYIEDNPDNMTTMRAILGADYRLTGASNGKEGLKKAAALKPRVILLDLSLPD
                     MDGFEVLEKLKSDAATRDIPVAAVTARAMKGDRRRALEAGCKAYVTKPVDPDEMRPVL
                     ERLLMS"
     gene            7890..8945
                     /locus_tag="P5541_06030"
     CDS             7890..8945
                     /locus_tag="P5541_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV71577.1"
                     /translation="MIILSIDDKKDNLVTLEAVLGDLLPKASVISVQNGLKGIEQARR
                     MKPDVILLDLRMPVIDGYEVTRKLRDIRETKSIPIIALTALDTSTENKVKALDAGVDA
                     FLVKPLDETELIAQLRAMFRLRESEAMLQKERDILEDKVRERTRELEMSSDALERALE
                     ETVYVLAKTVESKDPYTSGHQRRVAELGERIATRLGLDEFVRKSVFMAGMIHDIGKIQ
                     VPSEILSKPGKISAAEFDIIRQHPEAGYTILKPVHFPWPVADIVLQHHERIDGSGYPR
                     GLKDGNIMIEARILSVTDVVEAMSSHRPYRPSLGIDQALEEIRQGSGTLYDPDVVAVC
                     LELFENEGFEFPEEVSF"
     gene            8942..9418
                     /gene="ybaK"
                     /locus_tag="P5541_06035"
     CDS             8942..9418
                     /gene="ybaK"
                     /locus_tag="P5541_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRV71578.1"
                     /translation="MKKTNAARLLEKSGVDFELWNYEVDPADVSARAVAAKIGLPPEQ
                     IFKTLVARGDRTGIMLACVPGDSELDLKALAAASGNRKTEMVHLNEVLPLTGYIRGGV
                     SPLGTKKTYPVFIDESALGFERICVSAGARGVQIFIRPGDLADAAGAKQAPIAKQE"
     gene            complement(9427..10134)
                     /locus_tag="P5541_06040"
     CDS             complement(9427..10134)
                     /locus_tag="P5541_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71579.1"
                     /translation="MNALRVEDLHVHYGTIHAVRGVSFHVDEGEIVSIVGSNGAGKST
                     IMWTLAGVTPSSKGTVELFGKPRPSKPHEIVAQGLALVPERRRLFSSLTVEENLVMGA
                     YRRKGDRGEKESLEKCFHLFPILRERIKQRSGTLSGGEQQMLAISRALMSDPGILLMD
                     EPTLGLAPLLVETVIETIVRLRDEGITVLLVEQNAARALEISDRGYVIEAGSIVKSGP
                     GPELSDDPDIRRVYLGG"
     gene            complement(10115..10915)
                     /locus_tag="P5541_06045"
     CDS             complement(10115..10915)
                     /locus_tag="P5541_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71580.1"
                     /translation="MSPRDPLLSVKGLSRDFGGIRAVDSFTFDVREGTITGIIGPNGA
                     GKTTVFNLVTGIYRPSAGSIMFCGEDIGGFRPDRIVRMGIARTFQNIRLFNRRSCLEN
                     VLTPLMQREKFSFLGALAGSPGNRNKDRKLRGRALELLSELGLERHASCQAGTLPYGL
                     QRKLEIARALATEPSLLLLDEPAAGMNPEETRSLARLISEVREKFQITILLIEHHMDL
                     VMNICSPVIVMNFGTLLATGTPGEIRENQAVVDAYLGKGKIKNERAAG"
     gene            complement(10912..11769)
                     /locus_tag="P5541_06050"
     CDS             complement(10912..11769)
                     /locus_tag="P5541_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV71581.1"
                     /translation="MDYILSIVILACINMIAVLGLSIFTGFTGLFSLGHAAFVAVGAY
                     TSAILTYYYEVPFILALAAGAAAAGAVSLIIGIPTLRAKLRSDYFAIAILGFGEALRV
                     LLENFEITNGARGLPGIESFTTLPIVLVCLTAGVFVARNFLRSRWGAACIAIREDPVA
                     AEMAGVRLFRTRLLSLVVSAVYCGLSGGLLAHFLSFIQPVMFTLLQSTQLLAAVIAGG
                     MGSISGPLIASFVFIALPEALRVAQMWRLVAYGLLLVLIMVYRPQGLMGYSELSTLIS
                     RILKRGEAR"
     gene            complement(11772..12644)
                     /locus_tag="P5541_06055"
     CDS             complement(11772..12644)
                     /locus_tag="P5541_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV71582.1"
                     /translation="MFLQTLITGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLG
                     AYAGLALATRLSLGFGLVLAGSIAAAGIIAVLNERLAYSSLRKRKAPSLYLMISAMGC
                     AVFLENLVYATIGSRFYAFPEFFGRQVINLGSSSISLLDVAAFLFALAAILALHLFIS
                     RTRTGIAIRAGVSDMTMCSLMGVDLDRLIAIVFLLAGGFAGVAGVFLGIKYLVYPTMG
                     WVTNKAYIAAVIGGLGSLPGALAGGLILGVVETFVSTYVSSVIRDVFSFTLLILFLVL
                     RPNGLLGQDSEEKV"
     gene            complement(12747..13901)
                     /locus_tag="P5541_06060"
     CDS             complement(12747..13901)
                     /locus_tag="P5541_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV71583.1"
                     /translation="MKKIVAVLGVLLVLTLAGSAMAADVVKIGHTVSLTGGASMWGQS
                     EARALDMLVKKINEGGGVLGKQIEIVRYDNRNDAVESVNVARRLVSDKVVAVIGPAQS
                     GNSIATAPVLEKAKIPMVVTTATNPYVTIDQKTDKVRPFAFRPCFIDPFQGTVAARFA
                     ITDLGAKKGAILYDVGSDYGQWLARYFEEAFIDKGGEIVAKEAFRTDELDYRAQLGKM
                     KDLAPDVIFIPTSQKEAAMAAKQARDLGIKATLLGTDNWGSPDLIELGGSAIDGGYFV
                     NLTDLADPDIQDFVAEYKAEFGEEPVLPNPVMAQDALIMIVDAIKRAESTDGTKMAEA
                     LSTTKDLTVTSGVVTIDPSNHNPLDKPAVIQQVDVPGKTFKFVKKYVSVE"
     gene            complement(13986..14738)
                     /locus_tag="P5541_06065"
     CDS             complement(13986..14738)
                     /locus_tag="P5541_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRV71584.1"
                     /translation="MSPEFAEQEYFVLHLLSVSAEPLGAGTIKEELARNGMDMSEASV
                     GRFIRDLDNRGITERVGFQGRVLTPLGEERLGELVSEREKIVSTGAFLEAVKSREKDM
                     LLEILVARRAIERELAALAAESRSEKDIAVLREILNRQNDLISRGEPMASTDPEFHRE
                     VARISGNKVLQSAIEIIRKSGEGASFFEYIRTRVGSRVGSDHEPVFRAIEAGDPDAAA
                     AAMALHIDNIIRDVRTYWKDHMRELARPGGGP"
     gene            complement(15037..16737)
                     /locus_tag="P5541_06070"
     CDS             complement(15037..16737)
                     /locus_tag="P5541_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRV71585.1"
                     /translation="MKAARALLEMLRGYGVEYVFGLPGETTLGLYNEWKQFEGIEHVM
                     ARDERHAVFMADGYARVSGKPGVCEAPSVGATHMIPGIVEAMKSCVPLIAITSDIPLH
                     MEKHNMLTGFDQTALFAPFVKESLTAFRGDEIPFLVQRAFRVAVSGCPGPVHLRLPMD
                     VLTQEISSSLPQAQFRFAQCPGTRPVAEHLDVEKAARALAQSKKGVMICGQGTLTSKA
                     WDEVKATAETFGLAVGTTINGKGSIPEMHPLSIGVIGARGSTHFSNSFLEEADLVFFV
                     GSSTDSVGTNGGILPGETGKIRFIHLNISEKELGNSFGGGILLHGDVKSTLRAILDFA
                     EREALPAEDRQKDILDRRSTPKRRAFGNEGPIDPRAATEAISELMPEKTVIVADPGMS
                     AVYPAAFWKIPEAGRFFLTNFAMGALGYGLPAAIGASCALPEGSTVLNFMGDGSFGFV
                     AGEMETLARLGKNIKVILFNNSNFGWIRGTNHFSFGGPDFSPSFSKIDYVMLADSFGI
                     KSYRASTLPELNSALEEFFADTGPAFLEMVAPSPEECVPPVPDWASKAKESGRGSDYW
                     "
     gene            complement(16769..17407)
                     /locus_tag="P5541_06075"
     CDS             complement(16769..17407)
                     /locus_tag="P5541_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV71586.1"
                     /translation="MTKRATPSHPILETIAERWSPRAFTKDIPSAGELASIFEAARWA
                     PSCFNGQPWRFIVSRADDPDTFGKALSCLREQNASWAQNAPVLGFLIASENFEYNGKP
                     NPWSDLDCGLAMGQLALQAQSMGWSTHIMAGFHPDRVREIFGVPEGFDPLVAFVVGKP
                     GDPKTLPQDLLEREVAPRERKSITETVFSPEWGKKYFHGQETSDQDNKTDMN"
     gene            17552..18943
                     /locus_tag="P5541_06080"
     CDS             17552..18943
                     /locus_tag="P5541_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV71587.1"
                     /translation="MKYDVIIVGAGPSGLFAARELAAKGKKVAIIDKGKKISERSCPL
                     KEGKTKTCAHCKPCNVICGWGGAGSYSDGKLTLTPEFGGNLESYIGRKELLELIDVVD
                     REFVRHGASMELFSPDPDFARDVAIRASTVGLRIIPAKIRHMGTDRSKDILQSMFEEI
                     SRSCDVIMDTTVESVVLKEGRAAGVMLSGGETVESPVVLMAPGREGSFWMEKTVKSLG
                     LKIKSLPVDIGVRVEIPSGIAEAITDQFYEVKAILDTPTFDDQVRTFCMCPRGEVTTE
                     YLAQHDILTVNGHSNRDGNGQTGNTNFAILVSTDFTEPFKDPNGYGSHIARLANMLGG
                     GVIVQRLGDLKHGRRSTPARIERGLVDPTLKTAEPGDLSFVLPYRHLKGILEMIQALD
                     TIMPGINGKNTLLYGVEVKFYSLKLDLDADLMTNIPGLFASGDGAGVTRGVIQASASG
                     IVAARGILKALRC"
BASE COUNT         4305 a         5079 c         5440 g         4251 t
ORIGIN      
        1 gtggtgagaa gggccggggg atcgttttat gagagtgcgt agtgaagtct tttccaaagg
       61 caactcgcct gaaatcgttc gtttctccgc ctgctacggg gcgaccgcac agtgccggta
      121 aaaaccaacc gctactgtgt gcttttcctg cccctccgcg acggctgcga aactcaccgg
      181 ctcctgagcc ttcggtaaag ccttcactcc gtcccttggc cttgccgcct cccgcttttg
      241 cccatgcctt cctgtcgcca gacgccagtc tcctgcctcc ctggtcttcc gcttcacgtc
      301 tcacgtctca cgtctcacgt cttacggctt ttttgccctt cctgcctcca gcctcccgtt
      361 tcctgtttcc ccccactcct gtttccccac cctccttgac tccccccaaa aaaagtgcta
      421 taaaattcct caatacataa aagcggaggc aacgatgaaa agaaacgtag cagtctcctg
      481 caacttcgga ttcgtttatt tttacgccag cgtgggaccc cctctgaggg ccggcgccta
      541 ggcgtctatg ccttaagagg ccggggtccc caaaagggga ccccggcctt tttttattca
      601 ttttcatcat atttcaaagg agctgggtgc agtggagaaa gcggagaagc agtcgagcgg
      661 tttggtcaga tcggtaagag ccatgtggga gaaagtcccc ttctaccggt ccaggatgga
      721 ggatatggga gtttgcccgg aagatatagt ttccgtggaa gatcttcaca agctcccctt
      781 tatgacgaaa agtgacctca gggacgccta tcccatgggg atccttgcct gcgacagaga
      841 tgatgttgtg cgcgtacacg cttcttccgg cacgacgggc aagcccacag tcgttcccta
      901 tacgagaaac gatattacga cctgggaaga atgcatgggg aattgtctca cgaccgcggg
      961 tgtaaccagg ggtgatgttt tccaggtcat cctgggatac ggcctcttca ccggagccct
     1021 tggtttccac tatggagcgg aaaaactcgg agccatggtc attcccaccg gcggaggttt
     1081 cacggggcgc cagctgatgc tgatggagga cctcggaacc accgtattca cgagcacccc
     1141 atcctatgcc cttcacctct gcgaggaggt gcgcaagaac ggcctgaggg aaaaactgcg
     1201 cctgcgcctc gcgatcctgg gcggagaggc ctggacggag cagatgaggg accgcataga
     1261 gaacgaactt ggcgttatcg ccctggattc ctacggactg tccgaggtca taggccccgg
     1321 ggtggcaatg gagtgccccg aaagggcggg gcttcaccca gactgggagc actttatttt
     1381 cgagatagtt gaccccgaga caggtcgagt tcttcccgag ggaaaggaag gagagctggt
     1441 agttacctcc ctcaccaagg aggccatgcc cgttatccgg tatcgtaccc gggatcttac
     1501 ccgattttta cccgggcggt gtccatgcgg acgggatgac atgcgtcttg aaaggataaa
     1561 gggtcgctgc gacgacatgc ttatcatcag gggtgtcaac gttttcccgt cccaggttga
     1621 ggctgtcctc ggtaagatcg aaggtctctc cctgcactac aggcttgatg tgttccagcc
     1681 caacgggctc aaggaactga acgtcctttg tgaggctgga cgggaacttg acgaggtcgc
     1741 catgggcagg atgcagaaag aggttacgaa aaaactgcat gagaacctgg gcataagggt
     1801 cggattcagc ctgctgtccc ccggtaccct ggagcgttcg gcaggcaagg ctgtaagagt
     1861 cacccaggtg gcgtaactaa aggttcaagg aggagagaaa gttgatggaa agagatatgg
     1921 tcgagaagag agaggtttgc tgcggttcct gtgtcaatga tagttcattg gtaaagccca
     1981 tcgacattga tgtcgatttc gaggggctgg ccattgcgtg gaaagaggtg agcgaaactt
     2041 ccggcaaaag agtttaatcc atgaaggcgc cgaagagggt cccgaaatcg gtggacccta
     2101 tttcggtctt ccggtaactg tcaaagatat tcctgcgtcc ctcgacaagg gccggaggac
     2161 ggacgccgaa cctgatagag agagatgcct cggccatggc ggccagtttg tcgcaggcat
     2221 cgataaggag tccgtccacc gggtcccggt cgtcggtgtt gtagattctg ttcatgtcgg
     2281 atgggtctat tccggaaacg atattcgagg tgagcgggtc acgggccctg ttcgagaact
     2341 cgtccttcgt gaggtagcgt atctcatctc tccatttttc ggggagcagg ggcagtagcc
     2401 tgttctccat ggcctcctgt tcgtattcct tgatgagccc ctcaagcccc cccaccgagc
     2461 gttttacggg ggagactata tcgcgggtga gaacctcggg gaggtcgtga aagagccctc
     2521 caaagaagtc gttgcgaagc ctccggggag aggctccctc ctccagggca gagaagaagg
     2581 agagggaagc cacgatcaag agatgcccca ggaccgatgt cgcgggaacc ctcggtatct
     2641 gggcccagcg aacctggaac cggagctgtc cgcagaggtc gatgaaggcg aacactccct
     2701 tgtcggccat tccctggcgc atgaggatct ccttcactcc cgagagatcg aggtgctcat
     2761 tgacctgatc ctcgatctcc tttcgtgttt cctcgattcc ccacagcatg ctgttctgtg
     2821 cgtatatcat gcggaattcc cactgggtgg ccaggtagtg tgcggccctg agtatgtgtt
     2881 tttcgcgcgc cgagaacccg ggttcttcca gataccggcg gaagcggccg aaaacccccc
     2941 ctgaaagagg tgaaagatcg ggagccgtct tctcgaggac ccactgattg agtttgggtc
     3001 cgtgatttgc catcatcctg tgaaataccg gaggttttat gtctgtcaga atgaccctct
     3061 ggaaaaactc gaagagtcca ccctcaacaa gggcgaccca gtcaacggtt tttccgttat
     3121 cctcctcgca gcgtgcgatg gtccaggcta tggtcatctt gtgagcctgc tttgccagtt
     3181 cggtgaactg tgccgggcgg gcgtggtcat tccacctggt catggaagca gccgagaaaa
     3241 gggtttcgac gagccccgga gttatcatcc ttttacccct tcctgtggtc cgatttctgt
     3301 agaatattat cgctatgatt gtataataag cgatacaaat tttactatcg gtggagagat
     3361 gaatatttcg ggatggttcg cggaattcaa cctggaggtc gacctgatat ttgaatacct
     3421 aaggcgggaa aggaaccttg atttctccgg ttatagtccc ctcatgatcg aacgctgcat
     3481 aaaaaaccgc cttgaggcct ctggagcctt ggacctgacc ggatatcttc tgatgcttga
     3541 aaacaacaga atggaactgg attccctcct cgattcactt accatcggcg tgagcgagtt
     3601 ttttcgtgac cccatggttt tctccgccat agaaactttt gtccttccga caatttttcg
     3661 agacaaggtt gtttccggag accacagcat caggatatgg tcagcgggat gctccacggg
     3721 ggaggaaccc tactcggtgg ccattctttc caaagaatct cacttcccgg gaaagttgcc
     3781 agtcgatatt gacattttcg gtaccgatat cgatgaaaag gatctggaaa cagcaaaaac
     3841 gggagagtat cccatggaga ggctgaacaa tgtacggatg ggtcttttcg gaaaatattt
     3901 cgagccccag gttgatcatt acagggtaaa aaaaggtatt cgcgatatgg tctgtttctc
     3961 ctaccatgat ctgctttcgc aaaaggaaca ggttccgggg atcagcgtct tcggtgcttt
     4021 cgacatgatc ttatgccgaa atgttttgat ctacctcgac aaaaaatacc aggagatcgt
     4081 actggaaagg cttgaccgtg ctctcaggcc ggggggtttc ctggtactgg gagaggcgga
     4141 atccctggat gccgcgaggc gaatgcgttt tggacagata tgtgattttt gcagggtctt
     4201 ccgcaagaat ccggttcacc gatacggggg agggtcatag tgatggtcga cagattgatg
     4261 ttccggggta tagcaaaaaa attccttttc tggttcctgg tggttgccgt tgtccccctt
     4321 gggatatccc tctgggttat aacgacgcag atgatcgggt cggcccggca gaacgtatcg
     4381 gaaaacctgg cagccataag ggacatgaag gtatcccatt ttcgccaatg gctggataac
     4441 aaacaggtca acgtaaaagt tcttgccgaa gatacggcgg tcagagatct tgtggagaaa
     4501 attgagacgg gtcaggggaa cagggaggaa cttctgcaga aagcaaggga ggttctagga
     4561 aggcagctct ctctaaagcc cgattttctt gattttttcc tggtcgatag caggaccggt
     4621 gttatatttc ttgcgacgga gccatcaatc gaggggctca accgatccgg ggacgcctat
     4681 tttacaggcc ctctggagaa gaacgggacc tacatcaagg atatctaccg ttccgaggcc
     4741 cttgggggag aaccgacaat gaccatatct actcccgttt actgccatca gcacggggga
     4801 gaacacatta ccgccgtgct tgtagggagg ctggatctgg aaaattctct ctatgccctt
     4861 ctgcgcgcgc gggaggggct tgggcaaacg ggggagatgg tccttgtcaa cagagaatca
     4921 atagccctta acactctcgc aaaggctccc gatgcgcccc tgaggatcaa gatcactgcc
     4981 gaacccgcca ggcttgcggc gcaaggcggg accggagtcg tcgaggagaa ggattacgcg
     5041 ggggaaaagg tcctttcggc ctatacgcac cttcccgaac ttggttgggg tttcgtggtc
     5101 aagatggacc agactgagat ctatgcgccc atcgcggcta tggtacgcag gatcctgata
     5161 acggggctgg cctttttctt actgaccgtg gctctcgctc tggccgtttc ccggggtctc
     5221 gcgaggcctc tggagtcgat ggcaaaaatt tccgaaaaaa ttgccagagg cgaactggac
     5281 gccagaaaca ccgatgaaga acggaacgac gagatagggg accttgccgg ctctatcaat
     5341 accatggcag aaagactcca ataccgtttc ggtcttcttg agggcctgtc cagtgtaacc
     5401 gaggccgcgg tgggcagtta cgacagggaa ggcttcgccc gggctgtttt tgaagtgctt
     5461 atggccagga tccatgcgca catgggcgtg ttctacgtcc tctctggcga cggcaaaacc
     5521 tttgagcctt ccttttcggc ggggatggaa ccgaaagcga gggaaccttt cgaccgcgag
     5581 acacttgaag gtattctggg tttttcgtct ctcagggatg acgtggttgt cataaggcag
     5641 attccggacg ccgctccatt tgttttccgg gggccaggcg gtgtcgtgga ggccagggag
     5701 ataatggcct tgcccctttt ggtctcgggg agccttgaag ggctgatatg ccttggatcc
     5761 ctgacatcct tccagtggga ggatgaagaa cttgtcagag aatgcaggca ctctctctca
     5821 acggggctcg acaggatact ggcatcggag cgaatgcagg agatgacggc ccagataaag
     5881 gccaggaacg aagaactggt cagccagaca gaggaactta cccggcagtc ggaagaactg
     5941 caggagcaga acgtggaact cgagcatcag cgcaaaatga tagaggaggc gaatcgtctc
     6001 aagagcgagt tcctctcgaa tatgagccac gaactccgca cgcccctcaa ctccatattg
     6061 gctctctcca gggtccttca gcgcaggggg ggccaaaaag aggaggacga ggcaaaatat
     6121 ctggacgtga tagagaggaa cggcaggcaa ttgctggacc tgataaacga tattctggat
     6181 ctctcgaaga tagaatcggg acgtgtggag atcaggccct cactcttttc cctcagcgat
     6241 acggtcatct cgctcctgga aaccctgagg ccagtagcca tagaaaaaga gatcttcctc
     6301 gaggcggata tcccgaaaga tctcccgctt gtgaaaaccg atgattccag actcagacag
     6361 attctcagaa atctcctgga caacgccgtc aagtttaccc ccgaaggggg agttgccatc
     6421 tccgtaagga acgaaggcgg ggaattcgtc atcagggtga aagatacggg cataggtata
     6481 ccaaaagaag aactcccctt tatttttgac gagttccgcc aggtcgatgg ttcctcctca
     6541 aggcagttcg agggtactgg tcttggtctt gccattgcaa aaaaatcggc ctggttgctg
     6601 gcgggtagac taaccgccga gagccgggag ggcgaagggt ctaccttcac cctgagtatt
     6661 cccatggagt tggattcctt ttcccagaaa gccattgagg gtccatttat ggccggagga
     6721 gaagggaaaa aggttatcgt agttgaggat gacgtatcgg tagcctcaac catatcttct
     6781 cttctttcaa ggataggcta cgaggctatt gtcgccagaa gcggccatga tgctcttcgc
     6841 ctggtccgtc aggtgaaccc cgtggccatg acccttgaca taataatgcc cgaaatggat
     6901 ggatgggaag tccttcagaa gatcaaggga gatcccgaga cttccgctct ccctgttgtt
     6961 attatttctg tctgtgacga ggtggatacg gcaaaggcac tgggagccga tggatacctc
     7021 tgcaagccgg taattgagcg ggatctttcc gccgagatcc aacgggtttg cggcaaggac
     7081 agcggaaggg tgatagtggc cggggaagca tctggccgtc ggactatcct gatggtggag
     7141 gataatgaca tagcggccag gcaggtgaaa cacctgatcg aagaggaggg catcgccttc
     7201 gtggagatcg ctcccggggg tcagcaggct ctggaataca tgaaagagaa cacccccgac
     7261 gccataatcc tggacctgat gatgcccgga gttgacggtt tctcggttct ggaccagttg
     7321 cgcggcaggg agaccacggc gggaataccg gttttgatat tgacggccaa agacctgaca
     7381 cgggaagatc tttcgaggct tcgcagcaac aatgttcagc aacttgttca gaaaggggac
     7441 gtcgacaggg aggagatcct gaggaagatc cggctcctgt tcggggagac ggtggccaca
     7501 aacggggata tccgacccga tgttctctat atcgaggata acccggacaa catgaccaca
     7561 atgagggcta tcctgggagc cgactacagg ctgaccggtg cttccaacgg caaagagggg
     7621 ctgaaaaaag ccgcggccct gaagccgagg gtgatacttc ttgacctttc tcttccggat
     7681 atggatggtt tcgaggtgct tgaaaaactg aaaagtgacg cggccacgag agatatcccc
     7741 gtcgcggctg tgacggccag ggcgatgaag ggtgatcgca ggagagccct tgaagctgga
     7801 tgcaaggcat acgtaacaaa gcccgtcgat cccgacgaaa tgcgccccgt acttgagcgc
     7861 ctgctcatga gttgatgggg gggtcctgag tgatcattct ttctattgac gacaaaaaag
     7921 acaacctcgt aaccctcgag gctgttcttg gcgaccttct cccgaaggcc agcgtaatca
     7981 gtgtccagaa cggattaaag gggatagaac aggcccggag aatgaaaccc gatgtgatac
     8041 tgctggacct gcggatgcca gtcattgacg gctacgaggt taccagaaag cttcgtgaca
     8101 taagggaaac gaaatcgatt cccatcattg cgctgacggc tctggacact tctaccgaaa
     8161 acaaggtaaa ggccctggat gcgggtgtgg acgcgtttct cgtaaaacct cttgatgaga
     8221 ctgaacttat tgcccagtta agggccatgt tccgtctccg ggagagcgag gcgatgcttc
     8281 agaaagagag ggacatactc gaggacaagg ttcgcgaacg gacccgggaa ctcgagatga
     8341 gttccgatgc cctggagagg gcccttgagg agacggtata cgtccttgcc aagactgtgg
     8401 agtcaaaaga cccgtacacc tcgggtcacc agaggcgggt ggcggaactt ggggagcgga
     8461 tcgctaccag attgggactt gacgaatttg tccgtaaatc tgtttttatg gcgggaatga
     8521 tccatgatat cggcaagata caggttccca gcgagatact gagcaaaccc ggaaagatca
     8581 gcgccgcgga gttcgacatc attaggcagc acccggaggc aggttacact atattgaagc
     8641 ctgtccattt tccctggccg gtggccgata tcgtcctgca gcatcacgag cgcatcgatg
     8701 gctccggcta cccgcgcggg ctgaaggacg gaaatatcat gatcgaggcc cggatactat
     8761 ccgtaactga tgttgtcgag gccatgtctt cccacagacc ctatcgtccg tccctcggta
     8821 tcgaccaggc tcttgaggag atcaggcaag ggtctggtac cctctacgac cccgatgttg
     8881 tagcagtctg tctcgaactc ttcgaaaatg agggttttga gtttcccgag gaggtctctt
     8941 tttgaagaag accaacgccg ccaggcttct ggaaaaatct ggtgtggatt ttgaactctg
     9001 gaactacgag gtggatcctg ccgatgtcag tgccagggct gttgccgcaa agatcgggtt
     9061 gcccccggag cagatattca agaccctcgt cgcaagaggg gacaggactg gcatcatgct
     9121 tgcctgcgtg ccgggtgatt cggaactgga cctcaaggcc ctggctgctg cttccggaaa
     9181 cagaaagacc gagatggtcc acctgaacga agtcctgcct cttaccggtt acatcagggg
     9241 aggggtatcg cctttaggca ctaaaaaaac ctacccggtc ttcatcgacg aaagcgcctt
     9301 gggttttgaa cggatatgtg tcagcgccgg ggcgagagga gtccagattt ttatcaggcc
     9361 cggagatctt gcggatgctg ccggagcgaa gcaggccccg atagctaagc aggaataggt
     9421 ataattttaa ccgccaagat agactctgcg gatatccggg tcatcggaga gttccggccc
     9481 gggaccggac ttgactatac tgccggcctc gataacgtaa cccctgtcgc tgatctccag
     9541 ggcgcgcgcg gcattctgct ccacaagaag taccgtgatc ccttcgtcac ggagcctgac
     9601 gatggtctcg attacggtct ccacgagaag aggggccagt ccaagggtag gctcgtccat
     9661 caggagtatc cccgggtcgc tcatgagtgc ccgggaaatg gcaagcatct gctgttcacc
     9721 gcccgaaaga gttcccgacc gctgtttgat cctttctctc aggatcggaa aaaggtgaaa
     9781 acacttctcc agagactctt tttcgcccct gtctcccttc cgtcgatagg ctcccatcac
     9841 caggttttcc tccacggtca gggaggaaaa gagccgacgt ctctccggaa ccagggcaag
     9901 gccctgggcg actatttcgt ggggttttga tggcctgggc ttaccgaata attctacggt
     9961 gccttttgaa gacggggtta cccccgcgag ggtccacatg atcgttgatt ttccggcgcc
    10021 gttggagccg actatagaga ctatctctcc ctcatccacg tggaaggaga ctcccctgac
    10081 agcgtggatc gttccgtaat ggacatgaag atcttcaacc cgcagcgcgt tcattcttta
    10141 tcttgccttt ccccaggtat gcatccacca cagcctggtt ttcccttatc tccccgggtg
    10201 taccggttgc caggagtgtc ccgaagttca ttacgataac aggtgagcag atgttcatca
    10261 ccaggtccat gtggtgctcg atcagcagta tggtgatttg gaacttttct cttacctcgg
    10321 aaataagcct cgccaggctt cttgtttctt cggggttcat gccggcggct ggttcgtcca
    10381 gcagcaggag cgaaggttcc gtggctaggg ccctggctat ctcgagtttg cgctgaaggc
    10441 catatggaag agtccctgcc tggcaggaag cgtgcctctc gaggccgagt tccgaaagga
    10501 gttccaaagc cctgccccgg agttttcgat ccttgttcct gttcccggga gatcccgcca
    10561 gtgctcccag gaaagagaat ttctccctct gcatgagtgg agtaaggaca ttctcgagac
    10621 agcttctcct gttgaagagt cgaatgttct ggaaagttct cgctatcccc atccgtacga
    10681 tcctgtcggg ccggaatccc ccgatgtcct ctccgcaaaa cattatgctt cccgcggagg
    10741 ggcggtagat gccggtaacg agattgaaga ccgttgtctt gccggcgccg ttgggcccga
    10801 tgatgcccgt gatagtccct tcacgcacat caaaggtgaa ggaatccacg gctcttattc
    10861 cgccgaaatc cctcgaaaga cccttcaccg aaagaagggg gtcccgcggg ctcatcgcgc
    10921 ctcacccctt ttcaatatcc tgctgatgag ggttgacagt tccgaatagc ccatcagccc
    10981 ctgggggcgg tagaccatga tcaggacaag caggagaccg taggcgacca ggcgccacat
    11041 ttgtgccacc cttagcgcct ccggcagggc tatgaagacg aaggatgcta taagggggcc
    11101 ggatatgctc cccataccac cagcgatgac ggcggccagt agttgggtgg actgcaggag
    11161 agtgaacatg actggttgga taaatgacag gaagtgtgcc agaaggccac ctgaaagccc
    11221 gcaatagacg gcgctgacaa cgagggagag gagtctcgtg cggaaaagcc ttacacccgc
    11281 catttctgcc gctacggggt cttcccggat cgctatgcag gccgcgcccc agcgcgaacg
    11341 gaggaagttc cttgccacaa aaactcctgc cgtaaggcaa accaggacta tcggcaaggt
    11401 ggtgaagctt tcaattcccg gaaggcccct tgctccgttg gtgatctcga aattttcgag
    11461 gagcaccctc agggcttccc cgaaaccgag aatggcgatc gcgaaataat cgctcctgag
    11521 ttttgcccga agggtcggga taccgattat gaggcttaca gctcctgcgg cggcagcccc
    11581 cgcggcaagg gccaggatga aagggacctc atagtaatag gtcagaatgg ccgaagtata
    11641 tgctcctaca gccacaaaag ccgcgtgccc cagggagaaa agcccggtga accctgtgaa
    11701 tatggacagg ccgagaacgg cgatcatgtt gatgcaggca aggataacga tggaaaggat
    11761 atagtccatg tctagacctt ctcctcgctg tcctgtccaa gcaacccatt ggggcggagg
    11821 accaggaaca ggatcagcag ggtgaaactg aatacgtccc tgataacgct tgagacatag
    11881 gtggagacga aagtctcaac gacccccagg ataaggcccc cggcaagcgc cccgggaaga
    11941 ctcccgagcc ccccaattac ggcggctatg tatgccttgt tggttaccca gcccatcgtg
    12001 gggtatacaa ggtactttat cccgagaaat actccggcga cacccgcgaa tcctcctgcc
    12061 agaagaaaga ctatcgctat gagcctgtcg agatcgaccc ccatcaggga gcacattgtc
    12121 atgtccgaga ctccggccct tattgctatg cccgtccttg tccggctgat gaaaaggtga
    12181 agagcaagta tggctgccag agcgaacaga aaggccgcca catcaagaag gcttatgctg
    12241 ctgcttccca ggttgatgac ctgccgtccg aagaactccg gaaaggcata aaaacgggaa
    12301 ccgatcgtgg cgtagacaag gttctccaga aaaacagcgc aacccatggc acttatcatc
    12361 aggtaaaggg agggtgcctt gcgcttgcgc aggctggagt aggcgaggcg ctcgttgagt
    12421 acggcgataa tccccgcggc cgcaatgctg cccgccagaa ccagcccgaa accaaggctc
    12481 agtctggtcg ccagggcaag tcccgcgtag gcgcccagca ttatcacggc accgtgagcg
    12541 aagttgctga aattgagcac gctgaaaacg agggagtatc ccacggccat cagggcgtag
    12601 atgccaccta tcgaaagacc cgtgatcaga gtctgaagaa acaatccggc ctgcacatcc
    12661 tctccgggtc cccgtccact ggaagggtat ctgaaagagg gagccgggcc ctttcaggtc
    12721 ggctccccga taaaaacctt tttctattat tccacagaaa cgtatttttt gacgaacttg
    12781 aaagtctttc cgggaacgtc gacctgctgg atcacagccg gcttgtccag ggggttgtgg
    12841 tttgaggggt ctatggtcac gaccccgctt gtcacagtca ggtctttagt tgtggaaagg
    12901 gcctccgcca ttttggtccc gtcggtgctt tcggctctct ttatggcgtc tactatcatg
    12961 atcagggcat cctgggccat gaccgggttg ggaagtaccg gctcctcgcc gaattcggcc
    13021 ttgtattcgg cgacaaaatc ctgaatgtcg gggtcggcga ggtccgtcag gttgacgaaa
    13081 tagccaccgt cgatggcgct gcccccgagt tcgataaggt ccgggctacc ccagttgtcg
    13141 gtcccgagga gggtcgcctt gatgccgagg tccctggcct gtttcgctgc cattgcggct
    13201 tccttctggc tggtagggat aaaaattaca tccggagcga gatccttcat cttgccaagc
    13261 tgcgcgcggt aatcgagttc gtcggtgcgg aaagcctcct tggcaacgat ctcgcccccc
    13321 ttgtctatga aagcctcctc aaagtaacgt gccagccact gaccgtaatc ggagcccacg
    13381 tcatagagga tggctccctt tttcgcgcca aggtccgtta tggcaaaacg ggcggcaaca
    13441 gtcccctgga aggggtcaat gaagcagggg cggaaggcga agggtcgcac cttgtctgtt
    13501 ttctgatcta tagtcacgta ggggttagtg gccgtggtaa cgaccatggg tatctttgcc
    13561 ttttcaagga ccggagcggt ggcgatggag ttgccgctct gtgccgggcc tattacggcg
    13621 accaccttgt cagacacaag ccgtcttgcg acgttcaccg attccacagc gtcgttgcgg
    13681 ttatcatacc gcacgatctc gatctgcttt ccgagaactc cgcccccctc gttgatcttc
    13741 ttcaccagca tatcaagggc ccttgcctcg gactgtcccc acatcgaggc tcccccggtc
    13801 agcgacaccg tatggccgat cttcaccacg tccgccgcca ttgccgaccc cgcgagggtc
    13861 agaacaagca gcacgcccaa taccgctacg atcttcttca actctttcta cctccctttt
    13921 tctctacgta taatttccct tagacaggat ccgtatcctt ttttaaccgg gttctgagtc
    13981 cctgttcatg gaccaccccc cggtctggca agttctctca tgtggtcttt ccaataggtg
    14041 cggacatccc tgatgatatt gtcgatatga agtgccatgg cggcggcggc cgcatcaggg
    14101 tcgcctgctt caatggcccg gaacacgggc tcgtggtcgg atccgaccct gctgccgacc
    14161 cttgtcctga tatactcgaa gaaagatgct ccctctccgc tcttgcggat gatctcgatg
    14221 gccgactgaa ggaccttgtt gccggagatc cttgccacct cccgatggaa ttccgggtcc
    14281 gtggaagcca tcggttcgcc tctggagata aggtcgttct ggcggttgag gatctcccgc
    14341 agcacggcga tgtccttttc agaacgtgat tctgcggcaa gagctgccag ttctctctcg
    14401 atggcccgtc gggccaccag gatctccaga agcatatctt tctcgcggga tttcacggct
    14461 tcaaggaatg ccccagttga aacgatcttt tccctctcgg aaaccagctc gccgaggcgt
    14521 tcctcaccca ggggagtaag cactctgccc tggaatccga ccctctcggt aattccccgg
    14581 ttgtccaggt cccgaatgaa acgtcccacc gaggcctcgc tcatatccat gccgttgcgg
    14641 gccagttcct cttttatggt tcctgcgcca agaggttctg ccgaaaccga aagaaggtgg
    14701 agcacaaaat actcctgttc agcgaactct ggactcatga ggcctcctga agggcgccgg
    14761 tcatcactga tgagcgactt ttcgcatcag tttatacctt tccccgcatt tgtcaaactc
    14821 gttcgggaag cgggaaggca aaaaacatag ggagaaagga aaagctccag gactttgtgg
    14881 ctcctggtgt tacagggttg accttgttgt tgttggggtc aatccagtca acttggtcaa
    14941 taaggccttt ggttctcccg aaaaaaggtt ttctctgcgg tgtatcaccc tctgcgtcct
    15001 catgtggtta gaaaggtttg acgggtgtca acgttattac cagtagtcgc tccccctgcc
    15061 ggactctttt gccttgctgg cccaatcagg tactggaggg acacattctt ccggggatgg
    15121 agccaccatt tcaagaaaag ccggtcccgt gtctgcaaaa aattcctcga gtgcggaatt
    15181 cagttccggg agtgtggagg ctctgtaact cttaataccg aaactgtccg ccaacattac
    15241 atagtctatt ttgctgaagg atggcgagaa atccgggccg ccgaagctga aatggttggt
    15301 gccccgtatc cacccgaaat tgctgttgtt gaaaagtatc accttgatgt ttttccccag
    15361 cctcgcaaga gtttccatct ctcccgcgac gaaaccgaaa cttccgtcac ccataaagtt
    15421 gaggaccgtc gatccttcgg ggagggcgca tgatgccccg atggccgcag ggaggccgta
    15481 acccagggcg cccatggcga agttggtaag gaagaagcga cccgcttcag ggatcttcca
    15541 gaatgcggca ggatacacgg cgctcattcc cggatccgct acgatgactg tcttttcggg
    15601 catcagttct gatatggctt ccgtcgccgc cctcgggtcg atggggcctt cattgccgaa
    15661 tgcccgcctt ttaggtgtcg accttcgatc caggatatcc ttttgccgat cttctgcagg
    15721 cagggcctct ctttcagcga agtccagaat ggcccgcagg gtgcttttta cgtccccgtg
    15781 gagaagtatt cccccgccga aagaattacc aagctctttt tcgctgatgt tgaggtggat
    15841 aaatcggatt ttccctgtct ccccgggcag gatacccccg ttcgtgccga cggaatcggt
    15901 gctgctgccg acgaaaaaga caaggtctgc ctcctccaga aaggagttgg aaaaatgagt
    15961 gcttccccgt gctccgatga cgccaatgga gagagggtgc atttccggga tgctcccctt
    16021 tccgttgatg gtagtcccga cagcaagtcc aaaagtctcc gccgtcgctt taacttcgtc
    16081 ccaggctttg gaggtcagtg tgccctggcc gcagatcatc acgccctttt tgctttgagc
    16141 cagcgcgcgg gccgcctttt ccacgtcaag gtgttcggca acggggcggg ttcccgggca
    16201 ttgtgcaaaa cgaaactggg cctgtggtag agaggaagag atctcctgag tcaggacatc
    16261 cattggcagt ctcaggtgta ccgggccggg gcagcccgaa acagccaccc gaaaggctct
    16321 ctgtaccagg aatggtattt cgtctcctct gaaagccgta aggctctcct tcacgaaggg
    16381 agcgaagagg gcggtctggt cgaagccggt gagcatgttg tgtttttcca tatgcagggg
    16441 tatatcgctg gtaatagcta tcagagggac gcaggatttc attgcctcga ctattcctgg
    16501 gatcatgtga gtagccccca cgctgggagc ctcgcacaca ccgggctttc cgctgacgcg
    16561 agcgtaaccg tctgccatga aaacggcatg ccgttcgtct cttgccatta cgtgttctat
    16621 tccttcaaat tgtttccact cgttataaag accgagggtt gtctcgccgg ggaggccaaa
    16681 aacgtattca actccgtatc ctcgcaacat ttccagaaga gcccgggctg ctttcatcga
    16741 agatattctc tcccgtatcg gatatggttc agttcatgtc tgttttattg tcttggtcag
    16801 atgtttcctg gccatggaaa tatttctttc cccattcggg agagaaaacc gtctcggtga
    16861 tgcttttccg ctccctggga gcgacctcgc gttccagcag atcttgtgga agggtcttcg
    16921 ggtcccctgg ttttcctacg acgaaagcaa cgagggggtc aaatccctct ggtactccga
    16981 agatctcgcg aaccctgtca gggtggaatc cggccataat atgggtgctc caccccattg
    17041 actgggcctg cagggccagt tgccccatcg cgagcccgca gtccaggtct gaccaggggt
    17101 tcggtttccc attgtattcg aaattctcgc tcgcaatgag aaaacccaga acaggggcat
    17161 tctgtgccca cgaggcattc tgttctctca ggcaggaaag ggccttgccg aaagtgtccg
    17221 gatcatccgc cctggagacg atgaaacgcc agggttgtcc gttgaaacat gaaggagccc
    17281 agcgggccgc ttcgaagatc gatgccagtt ctcccgcgga gggaatatct ttggtaaatg
    17341 ccctgggact ccatctctcg gcaatggttt cgaggatcgg atgggaaggg gtcgctcttt
    17401 ttgtcatttt atccaccctt tcgtcacgaa aatcagaggc ttttttgtgt cgcaagttca
    17461 ttgtatcaaa acatgttccg acgggtcatg atgtcgaaga gagttataat atcagtgtgc
    17521 ggattcagga aaaacctcga gaggtgagac aatgaaatac gatgtgatca ttgtgggagc
    17581 agggccttcc ggcctttttg cggcccgtga gcttgcggcg aagggcaaaa aggtggcaat
    17641 tatcgacaag gggaaaaaga tcagcgagcg aagctgcccc ctgaaggaag gaaaaacaaa
    17701 gacctgtgcc cactgcaaac cctgtaacgt aatttgcggc tggggtggcg cgggatccta
    17761 cagcgacggc aaacttacgt tgaccccgga attcggcggc aacctcgaaa gttatatagg
    17821 ccgcaaggag ttgcttgaac taatagatgt ggttgacagg gagtttgtca ggcatggagc
    17881 cagcatggag cttttctccc ccgatcctga ctttgccaga gatgtcgcca tcagggcaag
    17941 cactgtgggg cttcgaataa tccctgcgaa aatcagacac atgggtaccg acagatccaa
    18001 agacattctt cagagcatgt tcgaagagat atcccggtcc tgtgacgtga tcatggatac
    18061 cactgtggaa tcggtagtgc tgaaagaggg gagggctgcc ggggtaatgc tttccggcgg
    18121 agaaactgta gaatccccgg tagtgcttat ggccccgggg cgcgagggct ctttctggat
    18181 ggaaaagaca gtcaagtccc tgggactgaa gataaaatcc ctgcccgtcg atatcggagt
    18241 tcgtgtcgag atcccctcgg ggatcgcgga ggcaattaca gaccagttct acgaggtcaa
    18301 ggccatactc gatactccca cctttgacga ccaggtcagg accttctgca tgtgcccccg
    18361 tggagaagtg acgacagagt atcttgccca gcacgacatc cttaccgtca acgggcacag
    18421 caaccgcgac gggaacggcc agaccggcaa caccaacttc gcgatccttg tctcgacaga
    18481 ctttaccgaa cccttcaagg accccaacgg ttacggaagc cacatcgcaa ggctggcaaa
    18541 catgcttggc ggaggggtca tagtgcagag gctcggagac ctgaaacacg gccggcgttc
    18601 cactcctgcc aggatagaaa gggggctcgt tgaccctacc ctcaaaaccg cggaaccggg
    18661 agacctctcc tttgttctgc cctacaggca tctgaaggga atattggaga tgatacaggc
    18721 cctggacacg atcatgcctg ggataaatgg aaaaaacaca ctgctttacg gggtggaggt
    18781 caagttctac agcctcaagc tggatcttga cgccgacctc atgactaata ttcctggtct
    18841 ttttgcttcc ggcgatggcg caggggtgac ccggggagta atacaggcat cagcaagcgg
    18901 gatagtcgcg gccaggggga tattgaaggc cctgagatgt tagacgggag acgtaagacg
    18961 aaaagacgtg aatcgggaat cgaaaaaata aagtgcgtca acggtaaacg gtcaacggtg
    19021 aacggaatga acctgaagag caggcgtcag gaagctggag gcaggagact ggaag
//
LOCUS       DAOTIQ010000038        17471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3924_length_17471_cov_7.760278, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000038 DAOTIQ010000000
VERSION     DAOTIQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 17471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17471
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_17471_cov_7.760278"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..387
                     /gene="rrf"
                     /locus_tag="P5541_06085"
     rRNA            273..387
                     /gene="rrf"
                     /locus_tag="P5541_06085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1531..2271)
                     /gene="deoC"
                     /locus_tag="P5541_06090"
     CDS             complement(1531..2271)
                     /gene="deoC"
                     /locus_tag="P5541_06090"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRV71588.1"
                     /translation="MIDLTDIELLSQRARQKAARAEAGFYDRPSPNSIRESIDHTLLF
                     PAPVAEEISVLCEEAVRFGFASVCVPMTSLPEAVRILTGTGVRVGTVAGFPLGASPLS
                     IKASEIRWAIEHGADEIDTVINIPAARESNRQMIREELFALREAAQGKTLKVILEMPL
                     LNDTQIITTLRLAEQAGVDYVKTCTGFAGSVTYYHVALLRTAASATMKIKASAGIRTY
                     AAAEILMGLGADRLGTSSATKFLEPLSS"
     gene            complement(2268..2627)
                     /locus_tag="P5541_06095"
     CDS             complement(2268..2627)
                     /locus_tag="P5541_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71589.1"
                     /translation="MEENHRPTDRTVAENCARTASYVFESVSRSARLSSLATPEMQDM
                     FGEWVDMIGRQIISNIEIPGKIDVNAMAEEIGISTSTLLGLLLFLNRSGKISITEVGL
                     ENGCGSVDDICNCMKGK"
     gene            2782..3810
                     /locus_tag="P5541_06100"
     CDS             2782..3810
                     /locus_tag="P5541_06100"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRV71590.1"
                     /translation="MDILLLGLGNVGRAFCELVENKSALLKKKHGIDCRFVGIATRTR
                     GSVVIPDGISVRELLDREASTGRLSSGTQSDTASSMVADMDYDLLVVCTSTTVRSPEV
                     PMAYIRGALNRGINVITSNKGPLFLAAGELCDLARKKRARFLFEAAVMGGTPFFSFVR
                     HCLGGSRIISCEGVMNTTSNYILDLMRGGSSFDDALLDARRKGFAEADPTFDVEGWDS
                     AVKGSIIAQVLMNSSPVPLESMDIEGITQVSPGLLFETARSGGAVRLLTKIDSESGRV
                     SVSVGPEVLPDSNPLSRVHGATNGVLFVTDTLGEVFVAGGGVGPMHTAYSLLRDLIES
                     AEPGNAGF"
     gene            3797..6175
                     /locus_tag="P5541_06105"
     CDS             3797..6175
                     /locus_tag="P5541_06105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71591.1"
                     /translation="MRDFKDLPGKLLLLLGFCGVTLALISSVMKLPMLFAASASLLVF
                     SFLNTGLPLWAKMLLPAGIFSGGFMVAGFHPFEMMIFALLLTLLSLLWHESRKAILTE
                     SFLVEKMSLSLAGAVTPFEVAEVAVESFSAMEAMENVAVFVHDQKKRRLLLLSGTRSY
                     RAGVSVPVERGIIGRAYRERKSQFISDVMNDPDYIDIGPASESKASLPLIWNDSVAGV
                     LNVEFLSARDMTPFRLRLIEKIVPILASALAISTRRERTEKILRRVLDSYREQKSRRR
                     WLAARQEREEQLNRSNEEREKLALLLLEIFREPGSLIEVESLCGKLVEQIFEKLGYPN
                     IYIVIRDNAKNGHGAGKTFRLAAYCGLSQAQYVHVLMDDNLKGIWGHVIATREAYLCR
                     NCAEDPAYIMGNPATRSELAVPIALRDTVWGLIDLQSDETDAFSEEDVKVLSFIATNL
                     AILFENSNAISQLEERGDRMRLLHDVVQSLALSPTVDDLCERVVESVSQKMGFEAASI
                     YRATDGEIPQLVASSAIPSSEYPSVNARMTRNTGLVGKTAREGKLRNTPDVYLDDSWI
                     PIVPSARSQLDIPIESGGTIFGVLVFEDVDVNAFSSMDEELFSIMARHIAVAWKMHTI
                     LDSLKEQALRDPMTGLWNSRYLRQRIEEEISRSKRHGSPFSVVMIDVGNFKSINDRYG
                     HLEGDYVIQEIGKKILAAVRQYDALARYGGDEFAIVLPDTSRQDAEKLAARIGVMDIV
                     TSTGDPEKIFLDSGVAVYGEDGEDVVGILKKADRSMYRMKETREREKRKGIL"
     gene            6176..7003
                     /locus_tag="P5541_06110"
     CDS             6176..7003
                     /locus_tag="P5541_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV71592.1"
                     /translation="MISVDLHTHSTFSDGTFSPEALARRAAKRHIAIISLTDHDTTSG
                     LDRFMKACRKEGVQGIPGVELSAEASFTLHILGYRIDIEEKVLQEKLESLRVGRDKRN
                     FQIVAKLRELGAEITMEDVEAESGGEVIARPHIARVLVRKGFVKDPASAFKRFIGRDC
                     PAFVPRVRLDPEECLSVIRGAGGLPVLAHPGLMALDEPVMLELIGRLSDKGLWGLECI
                     SGHHGSGEIFYWLKIANRFGLFPTAGSDFHGLDRPGTDLGVSVTETLLPWARLGARI"
     gene            7084..8118
                     /gene="ltaE"
                     /locus_tag="P5541_06115"
     CDS             7084..8118
                     /gene="ltaE"
                     /locus_tag="P5541_06115"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRV71593.1"
                     /translation="MNIIDLRSDTVTRPTEEMREKMASAEVGDDVYGEDPTVNRLEEE
                     SAAVTGHEAGLFVTSGTQGNLVALLTHCSRGDGAILGSNSHIYIYEGGGLSAMGGVLP
                     LIAPDTEGIVSPESIDGWCRPSNVHFAPARLLCLENTHNRAGGVAVSVESFGASVDMA
                     RSRGLSIHLDGARVFNAAVARGVDVKEYTARVDSVQFCLSKGLGAPVGSMLCGSSAFI
                     GAARHWRKRLGGGLRQAGVIAAAGLVALDKMTKRLGEDHANADLLSRLLTSGGLSVEK
                     VEMPTNMVYVSLDKSGPDAETLAGKCASRGILFGAVSERRFRLVTHFGITSKDVESAA
                     RIILEELKDS"
     gene            8118..9476
                     /locus_tag="P5541_06120"
     CDS             8118..9476
                     /locus_tag="P5541_06120"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRV71594.1"
                     /translation="MDRPERSEVESIARLLIDGAMSRGASMADVMYTCGNSAHFSLRD
                     AMPEKDSCGSSLRVGCRAIDREGRQGVADMNSLMRKNLETLLDDCMSNCAASEPEQDL
                     SLYRGSATGTEVPVFDPEVVSLSHEEREARCFEMHRLTADLDERIVSVRQASFVSGFG
                     ETLYASSEGVMKWQKDTFAACSVTVVMADGENHEMGGASSEGTRIGDVDHLSIAREAV
                     ERTAMIIGGTPLVTGVYDVVLDPDASASLVESIGELFLASAVYKNRSLLKGRMGEKIG
                     SGSLSIVDDGTIPWRTGSSSWDGEGVPSSRTVLMDRGVVSSYLYDLKYARRFKAKPTG
                     NASRSPGSLPGVGFSNLHLLPGERKIKDILSDNVGGILVSELMGVHTIDPVSGDFSLG
                     IKGALIEGGGVPGRAVAGMTIAGNLVTLLENITEIGSELRFFGDIGACPVVVKSVSMA
                     GC"
     gene            9484..11106
                     /locus_tag="P5541_06125"
     CDS             9484..11106
                     /locus_tag="P5541_06125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRV71595.1"
                     /translation="MLFLIAAGVAALPVPMSEAADQWELVEGDSVLGKVPVLAQNGYF
                     FVAVDEAARLLSLGAEEKGETLVLRGPGGNLQIFPGAAAAVTSGEIIPISHEVLRSRG
                     HWWVEPHSTLSMISRVSGKDGTSGVLSWRGEGSGQQTLVSYTPLQEPRKSPVSSGNLS
                     PVTVRSIRWGRQDFGLRVVLDLSAPAAVKVQTDKGSVTLSIPGILAKGTPGAVSPYPS
                     EIRTGVTQFGDRAVLVFAHNSSRVKHFSLDSPHRLVVDFYGPLPLAAGTAPQLPEEVP
                     APGSTETAVETEPSISTPLPGPVTTTVHPVKGPNKGVRTVVIDAGHGGKDPGAVANGI
                     REKDINLKVALLMANILKKQGLRVVLTRSTDVYLKLADRTNIAVRENADVFVSLHCNA
                     LPKGRNAEGVEIYLMALPSDKHAMELALIENRELVDGGMEKDEASDKRTRTLLKILGD
                     MQQNVKITESTSFAEVLYRTGESRKLPMRRVAQAPFYVLRGAAMPSVLVEMGFLTNLT
                     EASKLKNAKYQQQMAEALSAGIVEFLKRNDYN"
     gene            11154..11837
                     /locus_tag="P5541_06130"
     CDS             11154..11837
                     /locus_tag="P5541_06130"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRV71596.1"
                     /translation="MTDLRDYERRTRRPLDDEDEDERYLEEEENGKAPVVFRVAAWVS
                     LVVLLFAAGYWGTSMILKYLDKKQIIVQEQVVSDTDGARDVLRDNSASGLPGRKSGFD
                     IFIPRGEVLVSSPVTHVSGLMEDDVKAVIMNLLETMKAENTISEQVRVLHVFRNGDLL
                     YLDLNDRFLSALENMSSGKAALVMTSIVRSVVHNFPPVVKVRFLINGKDPELKKPVDL
                     TKPWQLKTS"
     gene            11816..12619
                     /gene="rph"
                     /locus_tag="P5541_06135"
     CDS             11816..12619
                     /gene="rph"
                     /locus_tag="P5541_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRV71597.1"
                     /translation="MAAEDILTPRIDGRSPDDLREVTITRGVNLFAEGSARVSFGNTV
                     VLCTASVEEKVPAFLRGTGRGWVSAEYSMLPRATKERTQRDSTRGRISGRSHEIQRLI
                     GRSLRSAVDLSSLGERTLWIDCDVIQADGGTRTAAISGGFVALVDALRHLYAKGEISC
                     IPLAAFVAAISVGKTDGILVADLCYEEDSLAEVDCNIVMNEFGDFIEVQGTAEGTPFP
                     RQDLDRMLDLAEKGIRSIIGVQKKVLDLDEAEMKAIEDARDRICKLQQG"
     gene            12582..13175
                     /gene="rdgB"
                     /locus_tag="P5541_06140"
     CDS             12582..13175
                     /gene="rdgB"
                     /locus_tag="P5541_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRV71598.1"
                     /translation="MREIVFASSNRDKLRELESLLGSSGILLLFGPDLYGAEIKVEEN
                     CSSYAGNAMQKAAVWARVMGIPALADDSGLEVRSLDWGPGVRSARVASDDVGRVSWLL
                     RRMSGKSDRSARFVAALALVDPSAGEWILAEGICNGEISSEPSGNEGFGYDPVFIPDG
                     YSKTIAELGPEVKSGISHRAIAARAISCMLENRSMVE"
     gene            13217..13444
                     /gene="secG"
                     /locus_tag="P5541_06145"
     CDS             13217..13444
                     /gene="secG"
                     /locus_tag="P5541_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRV71599.1"
                     /translation="MKVFLTVVHIIISISVMAVILMQQRKQGGFTGVFGGGTQQDAAG
                     GQWQRFSVLTKATIFLMALFLVTSLVLVLMH"
     gene            13461..14501
                     /locus_tag="P5541_06150"
     CDS             13461..14501
                     /locus_tag="P5541_06150"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRV71600.1"
                     /translation="MGFSRLERLSDIAGVEAEGDRLFSATHEEIMSGRTTDVYFLKTR
                     DILESCGRLDAPVVAEIFARKSGVFAGIPEVLRLLEGSGVEILSLKEGDLFGPKEVVA
                     RISGPYSAFGLFETVILGMLASSTGWATAARECVDAAEGKPVLSFGARHVHPAVAPVM
                     ERAAVVAGGCVGASCILGAQLAGVQPKGTIPHAAILIAGDTLEVARVYDRSLPEGEPR
                     IILVDTFRDEAEESLRIYEALGERLSGIRLDTPSERGGVTPDLVSEIRSRLDMAGAGD
                     VEVIVSGGLNPERIRILSAAGAASFGVGSYIAHAAPRDMTMDLKVVDGKPLAKRGRIP
                     GITENPSFSRML"
     gene            14651..15088
                     /gene="rplM"
                     /locus_tag="P5541_06155"
     CDS             14651..15088
                     /gene="rplM"
                     /locus_tag="P5541_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRV71601.1"
                     /translation="MAVNTTSTYVAKKEQVERKWFVVDATDKVLGRLAAQIAMILQGK
                     NKPTYTPHVDTGDFVVVINADKVKITGRKLERKFVYRHSGYKGSLKGTSYGTFMEKYP
                     DRLVERVVKGMLPKNKLRFEKKLKVYAGADHPHAAQKPEPLEL"
     gene            15118..15513
                     /gene="rpsI"
                     /locus_tag="P5541_06160"
     CDS             15118..15513
                     /gene="rpsI"
                     /locus_tag="P5541_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRV71602.1"
                     /translation="MKQSEYYWGTGRRKAALARVRVRSGSGTVKINERSAEDYFPRVI
                     WQSEALEALKTSGLEGKVDVFVNASGGGLTGQSGAVRMGLARALLKMNPDLRPALKKS
                     GLLTRDSRMVERKKFGQKGARGKRQFSKR"
     gene            15888..17243
                     /gene="mgtE"
                     /locus_tag="P5541_06165"
     CDS             15888..17243
                     /gene="mgtE"
                     /locus_tag="P5541_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRV71603.1"
                     /translation="MKDRLDMLYRQIDSLIIEHKFTEAKETLSTMEPADIGEMLVDAP
                     PSRRVLIFRILQKDLAIDVFEYMEGPEREELLDSFTEREAAEIIEEMSDDDRTALLDE
                     LPAKTVKKLMQHLSPEERKIANRLLNYPPDSAGRIMTAEYIGLKASMTAMEALQHIRR
                     EARSKETIYTCFVMDSERKLKGVVNLEDIILAQAETPVSDIMDDDPVFVATVTDQEKL
                     AQIISRYDLHVLPVVDKEGRLVGIVTFDDVLDILEEEATEDFERMAGIQPVDDGYLET
                     GLLSLVKKRLPWLVICILAQAISASVLRRYSLTIESVVALTFFIPLLIGTGGNTGTQS
                     ATLIIRGMTLGDISVKDLGFIFFRESMAAILLGVPLGVLAIGRAYMLGTGPEVAVVVA
                     LALVSVIMIGNLSGVLLPFIARAVKIDPAVMSGPFITTVVDVMGLLVYFEIARLVLRV
                     G"
BASE COUNT         4071 a         4357 c         5014 g         4029 t
ORIGIN      
        1 atatctgcgt gaatcgtgag acgtgagatc aaaagcgtaa acggtaaacg gtaaaggcaa
       61 gccccgtgaa tcgtgagtcg tgaatcgaaa aggcaatagc taattctttg tcattctgag
      121 gaccaacggg acgaagcgga atccgccagc aggcggacgt tccgcgccct ttgcccaccg
      181 gcgggaatct ggtttaggtt cggtgaatcg taacggtaaa ggtcagggtc aaaagcatca
      241 caaaaccaaa tatgtgaaca caaggaaaag ttttcggtgg ccatggcgga ggggacacac
      301 ccggtcccat gccgaacccg gcagttaagc cctccagcgc cgatggtact gcggtctaaa
      361 ccgtgggaga gtaggacgcc gccggaaact tttctttttt tgtatgagac cgtgagacgt
      421 gagacgtgag acgaatggaa aaacaaggag acaggagact ggagactgga ggcagggaag
      481 ggcaaaagcc gtaagacgtt agacgtgaga attgagacga aaaagcaaag accgaaaaac
      541 gtaaacggta aacggtaaac ggtgaacggt gaaccaaaaa aacaggtaag gcacaatata
      601 tgaaccagaa cgaccatgga acaacaaaaa ccacgaaaat atataagggc cggaaagccg
      661 gataaagctt cccggccctt ttttgtgtca acggaagttg gtttgaggtt ttggtctgtt
      721 ttttgaactt accgctcacc gttcatcgtt cacgcagttg atccagtctg tcatcctgag
      781 cggagcgaag cggaacgtcc gcctaccggc ggggatctgg gcttgccttt gactttttcg
      841 attcacgatt aacgattcaa ggcccctctc accacagggg tcgcagaggg tgaagccgcg
      901 cagagaaaac cattcttttg tgaggaccgt ggacgcaaaa atttgtcatt ctgaacccga
      961 agggtgaagc ggaatccgcc agcaggcgga cgttccgcgc cctttgccca ccggcgggaa
     1021 tctggttttg cccagtctcc tttctcctcg gttttccgcc cctcctgtct ccagcagcca
     1081 gtctccagtc gacttgcttt tccgctcctc ccgttgaccg tttaccgttg acgcagttga
     1141 tccagtctgt catcctgaat ccgcgtatag tgcggatgaa ggatctggtt cgaaacctga
     1201 attagaaaca gattcttcgt catttcattc ctcagaatga caagcacttg cccttcatgc
     1261 ctccttgact gtctccttgc tttttcgccc ttcccgttca ccgttcactg ttgaccgttt
     1321 acgcctttcg gtctttgctt tttcgtctca attctcacgt ctaacgtctt acggcttttg
     1381 cccttccctg cctccagtct ccagtctcct gtctccttgt ttttccattc gtctcacgtc
     1441 tcacgtctta cggctttttg cccttccctg cctccagtct cctgatgccc gtctccagtc
     1501 gccatgcctt cccgattcac ggtttttctt ctaactactc aacggttcca ggaactttgt
     1561 cgcactggag gtgcccagcc tgtcggcacc gagacccatc agtatctcag cggcggcgta
     1621 agttcttatg cccgccgatg ccttgatctt catggtggca gaagcagcgg tccgcagtaa
     1681 ggccacgtgg taataagtta cgctgcccgc aaagccggta catgtcttta cgtagtcaac
     1741 acctgcctgt tccgcaagcc tgagtgtggt aatgatctgg gtgtcgttta gaagaggcat
     1801 ttccagtatt accttgagcg tctttccctg cgcggcctcc cggagggcga agagttcctc
     1861 tcttatcatc tgcctgttcg attcccttgc ggccggaatg ttgatgacgg tgtcgatctc
     1921 gtcggctcca tgttcgatcg cccagcgtat ctccgaggcc ttgatagaaa ggggagatgc
     1981 tccaagggga aaacctgcca cagtcccaac cctgacccct gtgcccgtca gtatacggac
     2041 ggcctccggg agggatgtca tggggacgca gaccgaggcg aacccgaaac ggaccgcttc
     2101 ctcgcaaagg acagaaatct cttctgcgac gggagcggga aagagcaagg tgtgatctat
     2161 ggattccctt atggaatttg gtgagggcct atcataaaaa cccgcctcgg cccgtgccgc
     2221 cttttgtcgg gctctctggg aaagcagttc aatgtctgtc aaatcgatca cttgcctttc
     2281 atgcaattgc agatatcgtc gacacttcca catccgttct ccagccccac ctcggttatc
     2341 gagatcttgc cggaacggtt caggaaaaga agaagaccca gaagtgtgga tgtcgagatc
     2401 cctatctcct ccgccatcgc gttaacatca attttcccgg gtatttcaat attggatatt
     2461 atctgacggc cgatcatatc gacccattca ccgaacatat cttgcatctc gggagtggca
     2521 agggaagata atcttgcgga tctggatacc gattcaaaaa cataggatgc tgtcctggca
     2581 caattttccg caactgttct gtcagtgggt cgatggtttt cttccaattg catcacgcct
     2641 ccaagtggtt atgcttgatg atctgctgta tcctcatggt tgcaaagcat caaccggatc
     2701 ctgtaaatat tcctgaaacc aatgatatca tagacttgac cggcaaccag gccgagtcaa
     2761 gtcatggggg tcgaaggaaa aatggatatt cttttgcttg gcttgggaaa cgttggaaga
     2821 gccttctgtg agcttgtcga aaacaagtca gccctgttga agaaaaaaca tggtattgac
     2881 tgtcgttttg taggcattgc cacccgtacc cgcggttcgg tcgtcatccc cgacggaata
     2941 tccgtccgcg aattgcttga cagagaggcc tctaccggaa ggttgagctc cgggactcaa
     3001 tctgacacag cttcttccat ggttgcggat atggattacg acctgctcgt cgtatgcact
     3061 tccacgaccg tccggtcacc ggaggttccg atggcctaca tcaggggggc tctgaatcgc
     3121 ggcatcaacg ttataacttc aaacaagggg cctctgtttc ttgcggccgg cgaactttgc
     3181 gaccttgcaa gaaaaaaaag ggcaagattc ctgttcgagg cggcggttat gggagggacc
     3241 cccttctttt cttttgttag gcattgtctg ggaggatccc ggatcatctc ctgcgagggt
     3301 gtaatgaata caacgtcaaa ctacatactg gaccttatgc gtggcggctc ctctttcgat
     3361 gatgctcttc tcgatgctcg aagaaaaggg tttgccgaag ccgacccgac tttcgatgtt
     3421 gaaggatggg acagtgcggt caagggttcc ataattgccc aggtgcttat gaactcaagc
     3481 cccgttccgc tggagagcat ggatattgaa ggtatcaccc aggtaagccc gggactcctc
     3541 ttcgagactg cgagatcagg tggggcggtg cgtcttttga cgaagatcga ttctgagtcg
     3601 ggcagagttt ccgtttcggt cggaccggaa gtgctgccgg attccaaccc cctttccagg
     3661 gttcacggtg ccaccaacgg ggtccttttt gttaccgata cccttggaga ggttttcgtc
     3721 gcgggcgggg gtgttgggcc tatgcacacc gcttactccc ttctcaggga cctgatcgag
     3781 tcggcggagc ccggcaatgc gggattttaa ggatctaccc gggaaactgt tgcttttgct
     3841 tggtttttgc ggggtaaccc ttgccctgat ttcctccgtc atgaaactac ccatgctgtt
     3901 tgccgcatct gcaagccttc tggttttctc atttctgaat acgggactcc ccctgtgggc
     3961 gaaaatgctt ctcccggcag gaatattttc cggcggattt atggtcgcgg gattccatcc
     4021 tttcgaaatg atgatctttg ctcttttgct gacccttctc tccttgcttt ggcatgaaag
     4081 cagaaaagcg atactgacgg aaagcttcct tgttgaaaaa atgagcctgt cccttgccgg
     4141 agcggtgacc ccttttgagg tagccgaggt cgctgtcgaa tccttcagcg ccatggaggc
     4201 catggaaaat gttgcggtat ttgtccacga ccagaaaaaa agaaggttgt tactcctttc
     4261 ggggacgaga agttacaggg ccggagtgtc cgttcccgtt gaaagaggca tcatcggaag
     4321 ggcatacagg gaaagaaagt cccagttcat ttccgatgta atgaacgacc ctgattacat
     4381 tgatataggc cccgcatcag aatccaaggc ttcccttccg ctgatatgga atgattcggt
     4441 tgcaggtgtc ctgaacgtcg agttcctttc tgcaagggac atgactccct tcagactcag
     4501 actcattgag aagatcgtgc ccatacttgc ttcggccctt gccatctcga cgcgccggga
     4561 gaggaccgaa aagatcctga ggcgagtcct tgactcttat cgcgaacaga aatcccggcg
     4621 tcggtggctg gcggcgaggc aggaaaggga ggagcagctg aacagatcca acgaggagag
     4681 agagaaactc gctcttctgc ttctggaaat attccgggag ccggggtccc ttatcgaggt
     4741 cgaatccctt tgcgggaaac tggtcgagca gatctttgaa aaactcggct atcccaatat
     4801 atacatcgtg atcagggata acgcgaaaaa tggccatgga gcgggaaaaa cattccgtct
     4861 tgcggcgtac tgcggccttt cccaggccca gtatgtgcat gtcctgatgg acgacaacct
     4921 caagggaata tgggggcatg tcattgccac cagagaagct tatctctgcc gcaactgcgc
     4981 cgaagaccct gcctacatta tgggcaaccc cgctaccagg agtgaacttg cggtcccgat
     5041 agctctgagg gataccgtct ggggtctaat agatctccag agcgatgaga cggatgcttt
     5101 ctccgaggag gatgtcaagg tactttcctt cattgccaca aaccttgcga tacttttcga
     5161 aaactcaaat gccatttcgc agctggaaga acgaggagac aggatgaggt tgctccacga
     5221 tgttgtccag tcacttgcgc tttcgccgac ggtggatgat ctttgcgaaa gggtggttga
     5281 atcggtctcc caaaagatgg gtttcgaggc cgcttcgatc tacagggcca ccgacgggga
     5341 gattcctcaa cttgtcgctt catcggcgat cccttcttcg gaataccctt cagtgaatgc
     5401 ccgaatgacc agaaatactg gcctggtggg aaagactgcc agagagggca aattgcgcaa
     5461 cacccctgat gtataccttg acgactcatg gatcccgata gtcccctcgg caagaagcca
     5521 gctggacata cccatagagt caggagggac gatattcggg gtgctggtgt tcgaggatgt
     5581 cgatgtaaat gccttttcct ccatggatga ggaactcttt tcgataatgg cccggcatat
     5641 tgccgtggcg tggaagatgc atacaatact tgattcactc aaggagcagg ccctcaggga
     5701 tcccatgacg ggactctgga actccaggta cctgaggcaa agaatagagg aggagatatc
     5761 cagatcaaaa aggcacggaa gccccttttc cgtcgtgatg atagatgtcg ggaacttcaa
     5821 aagcataaat gacaggtacg gccatcttga gggagattac gtcattcagg agatcgggaa
     5881 aaaaattctt gcggcggtac gtcagtatga tgcccttgcc cgctatggag gggacgagtt
     5941 tgcaatagtc cttccggata cgtcaaggca ggatgcggag aaacttgccg cacggatcgg
     6001 tgttatggat attgtgacct caacgggtga tccggaaaaa atctttctgg attccggggt
     6061 cgctgtatat ggagaagacg gcgaagacgt cgtcgggata ctcaaaaaag ccgacaggtc
     6121 catgtacaga atgaaagaaa ccagagaaag ggaaaagcgg aaggggattt tatagatgat
     6181 atccgtcgat cttcatacac acagcacctt cagcgatgga accttttcac ccgaagctct
     6241 tgcccgacgg gcggcgaaaa gacatatagc aataatcagc ctgacggacc acgataccac
     6301 cagcgggctt gaccgtttca tgaaggcctg ccgcaaggag ggggttcagg ggatccccgg
     6361 ggtggaactg tctgccgagg cctcttttac acttcatatt ctcggttaca gaatagatat
     6421 tgaagagaag gttctccagg aaaaactcga aagcctcagg gtaggcagag acaaaagaaa
     6481 ctttcagata gtcgcaaagc tcagggaact gggagccgaa atcaccatgg aggatgtgga
     6541 ggccgaatcc ggaggagagg tcatcgctcg tcctcacata gccagggtgc ttgtgcgcaa
     6601 ggggtttgta aaggatccgg catcagcgtt caaaaggttc ataggaagag attgccctgc
     6661 ctttgtgccg agagtcaggc tcgatcccga ggagtgtctt tcggtgatac gaggtgcggg
     6721 tggactgccg gtcctcgctc atcctggatt gatggctctg gatgagcctg tgatgctgga
     6781 actgatcggc aggctatctg ataaaggact ttggggtctt gaatgcatat cgggtcacca
     6841 cggctcgggg gaaattttct actggctcaa gatagctaac aggttcgggc tctttccgac
     6901 ggcaggttct gattttcacg gactggatcg cccgggaacg gacctcgggg tttctgtcac
     6961 agaaacactg cttccctggg caaggctggg ggccaggata tgagcccgta ttggtgatat
     7021 catatctgca atgatcggcc agaggctcga cacaggtctt ttttaagggg ggtttcagta
     7081 tttatgaata ttattgatct cagaagcgat accgttacaa gacctaccga ggaaatgaga
     7141 gaaaaaatgg catctgccga ggtcggcgac gatgtttacg gggaggaccc aacggtcaac
     7201 aggctggagg aggaatccgc cgccgttacc gggcatgagg cggggctctt tgtcacctcc
     7261 ggcacccagg ggaacctcgt ggcacttctt acccactgtt cccgtggcga tggggccatt
     7321 cttggttcca acagccatat ctacatctac gaggggggtg gtctttcggc catgggcggt
     7381 gtccttcctc ttattgcgcc cgataccgaa ggcatagttt ctccggaaag tatagatggc
     7441 tggtgcagac cctccaacgt gcatttcgct ccggcccgcc ttctctgtct ggaaaacacc
     7501 cacaacaggg ccggaggtgt tgcggtctct gtggagtcct tcggcgcctc tgtggatatg
     7561 gccagaagcc gggggttgtc tatacatctt gacggggcaa gagtctttaa cgctgccgtg
     7621 gcaaggggtg tcgacgtcaa ggaatacacc gccagggtgg attcggtcca gttctgcctc
     7681 tccaagggac ttggagctcc cgtcgggtcc atgttatgcg gcagttcggc tttcattggt
     7741 gctgcgagac actggcgcaa gcgcctcgga ggcggactgc ggcaggccgg ggtaatagcc
     7801 gctgccggtc ttgtcgccct ggataaaatg acaaaaagac tcggggaaga tcatgcgaac
     7861 gcggacctgc tttccaggct gcttacttcg ggaggcctgt cggtcgaaaa agtggagatg
     7921 cccactaaca tggtctacgt cagtctcgat aaatccggcc cggatgcgga aacccttgcc
     7981 gggaaatgcg catcccgggg gatattgttc ggggcagtgt cggaaagaag gttccggctg
     8041 gtgactcact ttgggataac ttcaaaggat gtggaaagcg cggcccggat aatactcgaa
     8101 gagttgaaag attcctgatg gaccgcccgg aaagaagcga ggtcgagtcc attgcccggt
     8161 tgcttataga cggggcaatg tccagggggg cctccatggc cgatgtcatg tatacctgcg
     8221 ggaactcggc acacttctct ctcagggatg ccatgccgga aaaggacagc tgtggatcct
     8281 cgctgcgagt aggctgcagg gccatagaca gggaaggcag gcagggagtt gccgatatga
     8341 acagccttat gaggaaaaac cttgagacgc tgctggacga ttgcatgtcg aattgcgcag
     8401 cttccgaacc ggagcaggat ctctcccttt acagagggtc ggctaccggc actgaggttc
     8461 cggtctttga ccctgaggtt gtttctcttt cacatgaaga gagagaggcc aggtgtttcg
     8521 aaatgcatcg cctgaccgca gatcttgacg aaagaatcgt ctctgtcagg caggcttcat
     8581 ttgtcagcgg tttcggcgag accctgtacg catccagcga aggggttatg aagtggcaaa
     8641 aagacacatt cgctgcctgt tccgtcaccg tggttatggc cgatggtgag aatcacgaga
     8701 tgggtggagc ttcttccgaa ggtacaagga tcggagacgt cgaccacctt tcgatagcca
     8761 gggaagcagt agaaaggacg gccatgataa ttggtggaac acctctcgtg acgggagtat
     8821 atgatgttgt gctggatccg gatgcgtcgg cctctcttgt tgaatcaata ggagaacttt
     8881 ttcttgcttc cgcggtctac aaaaacaggt ctctcctgaa aggaagaatg ggagaaaaga
     8941 tcggaagcgg gtctttgtcc atcgtggatg acgggacgat tccctggaga acaggttcat
     9001 cttcgtggga tggtgaaggt gttccctcca gcaggacagt tcttatggac aggggagtgg
     9061 tctcttctta tctctatgat ctcaagtatg cccgaaggtt caaggccaag ccgacgggca
     9121 acgcatccag aagccccggc tcacttccgg gagtcggttt ttcaaatttg caccttctgc
     9181 cgggagaaag aaaaataaaa gacattctct ccgacaatgt gggggggata ctggtgagtg
     9241 aactcatggg agttcacact atcgatccgg taagcgggga tttctccctc gggatcaagg
     9301 gggctcttat cgaaggggga ggggtaccgg gaagggcggt tgcgggtatg accatagccg
     9361 gcaacctcgt tacgcttctc gaaaacatta ccgagatcgg tagcgaactc cgtttcttcg
     9421 gtgatatcgg agcttgtccg gtggtggtga aaagtgtttc gatggcgggt tgctaggatt
     9481 gtcgtgttat tcctgatagc agcgggggtt gctgctctcc ctgttcccat gtccgaagcg
     9541 gcggatcaat gggaacttgt ggagggagat tcggtgcttg ggaaagtacc ggtgctggcc
     9601 cagaacgggt acttcttcgt cgctgttgac gaggccgcca ggcttctgtc tcttggcgcc
     9661 gaggaaaagg gcgagaccct tgttctgaga gggcccggag ggaacctgca gatattcccc
     9721 ggggcagccg ccgcggtaac ttccggcgag ataatcccca tatcccacga ggtcctcaga
     9781 tcccggggac actggtgggt ggaacctcac agtactctgt ccatgatcag cagggtttcg
     9841 ggaaaagatg gcacttccgg tgtactgtca tggcggggag aaggttccgg gcaacagacc
     9901 ctggtttctt acacccccct tcaggaaccc cggaaaagcc ccgtatcttc gggcaacctg
     9961 tctcctgtca ccgtgaggtc gataaggtgg gggaggcagg atttcgggct tcgggtggtg
    10021 cttgatcttt cggctcccgc ggcggtcaaa gttcagacag acaaggggtc ggtcacgctt
    10081 tcgatacccg ggatccttgc aaagggaacc cccggagcgg tttcccccta tccttcagag
    10141 ataagaacgg gagtgaccca gttcggagat cgggcggtcc ttgtcttcgc tcacaactct
    10201 tccagggtga aacatttcag ccttgactct ccccacagac tcgtggtgga tttttacggc
    10261 cccttgcctc ttgctgcagg gacagccccg caactgccgg aggaagttcc ggcccctggt
    10321 tctacagaaa cagccgtgga aactgagcca tcgatctcca ctcctctgcc tggacccgtc
    10381 accaccaccg ttcatcctgt aaaaggtccc aacaaggggg tcagaacggt ggtgatcgat
    10441 gccggtcatg gggggaagga cccgggggcg gtggcgaacg gcataagaga gaaagacata
    10501 aacctcaagg ttgcccttct tatggcgaat attctcaaaa aacagggact gagggtcgtc
    10561 cttacgagga gcaccgacgt ttatctgaaa ctggccgaca ggacaaatat tgctgtaagg
    10621 gagaatgccg atgttttcgt gagccttcac tgcaacgccc ttccaaaggg aagaaacgcg
    10681 gagggggttg aaatatatct catggctctt ccttccgaca agcacgccat ggaactggct
    10741 cttatagaaa accgggaact ggtcgacgga ggtatggaga aggacgaagc ctcggataaa
    10801 cgtacaagaa ccctgctgaa gatcctcggg gacatgcagc agaacgtcaa gataaccgaa
    10861 agcacctctt tcgccgaggt cctttatcgg accggcgaat cccggaaact tcccatgaga
    10921 agggtggctc aggctccttt ctatgtcctc aggggagccg cgatgccgtc ggttctcgtt
    10981 gagatgggct ttctcacaaa tttgacggag gcatcaaaac ttaaaaacgc gaaataccag
    11041 cagcagatgg ccgaagccct ctcagccggg atcgtggagt ttctgaagag aaacgattat
    11101 aattgatcgc tgtccggcca gggtgaaacc gtgccgatac ggagggagat accatgactg
    11161 atctcaggga ctacgaaaga agaacgaggc gcccccttga tgatgaagat gaggatgagc
    11221 gctatctgga ggaagaagag aacggcaagg ctcctgtagt tttcagggtc gctgcctggg
    11281 tctctctggt ggttcttctg tttgcggctg gatactgggg gacctcgatg atcctgaaat
    11341 acctagacaa gaagcagatc atcgtgcagg agcaggtagt gagcgacacc gacggagcca
    11401 gggacgtact ccgggacaac tccgcttcag gtcttccggg cagaaaatcc ggtttcgata
    11461 tcttcatacc caggggtgaa gtacttgtat ccagccctgt cacccacgta tcggggctaa
    11521 tggaggatga tgtcaaagcc gtcatcatga accttctgga gaccatgaag gcagagaaca
    11581 ctatctcgga gcaggttcga gtcctccacg tgtttcgcaa cggagacctc ctgtatcttg
    11641 acctcaacga tcgctttctg tcggctctgg agaatatgtc atccggaaaa gcggctctgg
    11701 tgatgacatc cattgtccga agtgttgtcc acaattttcc ccccgtcgtg aaagtgcgtt
    11761 tcctgatcaa cgggaaagat cccgaactga aaaaacctgt agatctgacg aagccatggc
    11821 agctgaagac atcctgactc caaggatcga cggaaggtcc cccgatgatc ttcgggaagt
    11881 aacgatcact cgcggggtaa acctttttgc agagggttcc gcccgggtat ccttcgggaa
    11941 taccgtagtt ctctgcacgg catccgtgga ggagaaggtg cccgcttttc tccggggaac
    12001 aggtcgtggc tgggtgtcag cggagtattc aatgcttcca agggcaacaa aagaaaggac
    12061 ccagcgtgac agcacccgag gccggatcag cggcaggagc cacgagatac agcggctgat
    12121 aggaagatct ctaaggtctg cggtggacct ctcttctctc ggagaaagga ctctgtggat
    12181 tgattgtgac gtgatccagg ccgatggcgg gaccagaaca gcagcgatat ccggtggttt
    12241 cgtggccctc gtcgacgctc ttcgccacct ctatgccaaa ggcgagatca gctgcatacc
    12301 ccttgcggct tttgtggccg cgataagcgt ggggaaaacc gatggcattc tggttgctga
    12361 tctttgctac gaggaagata gccttgccga ggtggactgc aacattgtga tgaacgaatt
    12421 cggggatttc atagaggttc agggaacggc cgaagggact cccttcccga ggcaagacct
    12481 cgacaggatg ctggatctgg cagaaaaagg gatccgttcg ataattgggg tccagaagaa
    12541 agtcctggat ctcgatgaag cggaaatgaa agcgatagag gatgcgagag atcgtatttg
    12601 caagctccaa cagggataag ctaagggaac ttgaatcact gctcggttcc tccggtattt
    12661 tgttgctttt tgggccggac ctttatggcg cagagataaa ggtcgaagaa aactgttctt
    12721 cctatgcggg gaatgccatg cagaaagcag cggtctgggc ccgtgtcatg ggtattccgg
    12781 ccctcgcgga tgacagcgga cttgaggtcc gcagtctcga ttgggggcct ggcgtaagat
    12841 ctgcaagggt cgcttctgac gatgtcggga gggtctcctg gcttttgcgg cgaatgagtg
    12901 ggaaaagtga cagatctgcc cgttttgtgg ccgcccttgc gctggtggat ccttcggcgg
    12961 gagaatggat cctggccgaa gggatatgca acggagagat atcttcagag ccttcgggaa
    13021 atgaggggtt tggctatgat cccgttttta tccccgatgg ctacagcaaa accattgcgg
    13081 aactgggccc cgaggtgaag tccgggatat ctcaccgggc aatagcggca agggctattt
    13141 cctgtatgct tgaaaatcgt agtatggtag aataagcatt cgtttcatgg aacgaattgt
    13201 ttgggaggtg cagcgtgtga aggtttttct cacggtcgtc catattatta tatccatctc
    13261 agttatggcc gtaatcctta tgcagcaacg caagcagggt ggttttactg gcgttttcgg
    13321 tgggggaacc cagcaggatg cggccggagg tcagtggcag cgtttctccg ttctgaccaa
    13381 ggcaacaata ttcctgatgg cccttttcct ggtaacatcc ctcgtccttg ttcttatgca
    13441 ctgacccggg gatgagggtg atgggctttt ccaggctcga aaggctttcc gacattgcag
    13501 gagtggaggc tgaaggggac aggctgttca gcgcaactca cgaagagata atgtccggca
    13561 ggaccacaga tgtctatttc ctcaagaccc gggacatcct cgagtcctgc gggaggcttg
    13621 atgccccggt ggtagccgag atcttcgcga ggaagagcgg cgttttcgcc ggtataccag
    13681 aggttctcag gcttcttgaa ggaagcggtg tggagatact ctcccttaag gaaggggacc
    13741 tgtttggtcc aaaggaagtg gtagccagga taagtggtcc atattccgcc tttgggttat
    13801 tcgagactgt gatccttgga atgcttgcat cttccaccgg ttgggctaca gcggcccggg
    13861 agtgtgtaga tgcggcggag ggcaaacccg ttctctcctt cggcgccagg catgtccacc
    13921 ctgccgttgc ccctgttatg gaaagggcag cagtggttgc cggtggttgc gttggcgcaa
    13981 gttgtattct tggagcgcaa ctggctggag ttcagcccaa agggaccata cctcatgcgg
    14041 cgatcctcat agccggggac accctggagg ttgccagggt ctacgatcgt tctcttcccg
    14101 agggtgaacc caggatcata ctcgttgata cattccgtga cgaggcggag gagtcgttga
    14161 gaatatacga ggctctgggc gagaggctgt ccgggatccg tctggataca ccttccgaaa
    14221 gaggtggtgt aacgccggat ctggtctcgg agatacgcag caggctggac atggctggag
    14281 ctggagatgt tgaggtcatc gtttctggag gcctcaaccc ggaaaggatc aggatacttt
    14341 ccgcggcagg agcggcatct ttcggagtag ggagttatat tgcccatgct gcccccaggg
    14401 atatgacgat ggatctcaag gtcgtggacg gaaaacctct ggccaagaga ggaaggatcc
    14461 ccggaataac cgaaaacccg tccttttcca gaatgctctg atccccctgg aacaggtcgt
    14521 ttttccttga cacccgggga acgcttcgat agaataatca ccgctctgct tttttgctga
    14581 cagcgcaggg tatgtgaata tgtgtgtccg ccgtcaaatc ggcggccgga taaggagtgg
    14641 aataaagcaa atggctgtga atacgacctc tacctacgtg gcaaagaagg aacaggttga
    14701 aagaaagtgg tttgttgttg acgctacgga caaggttctc ggcaggctcg ctgcccagat
    14761 cgcgatgata cttcagggca agaacaagcc gacctacacc cctcacgttg acaccggtga
    14821 ctttgtggtt gtcataaatg ccgacaaggt gaagatcacg ggcaggaaac ttgagagaaa
    14881 gtttgtttat cgccacagcg gatacaaggg cagcctcaag ggtacttcct acggtacttt
    14941 tatggaaaag tatcctgatc gccttgtgga gcgtgtggtc aagggaatgc tcccgaaaaa
    15001 caaactacgc tttgagaaaa aactcaaagt ttatgccggt gcggatcatc cgcacgcggc
    15061 ccagaagccc gaaccgctgg aactttaatc cgggacggag aaaggaggaa aaccgtaatg
    15121 aaacagtccg aatattactg gggcaccggc aggcgcaagg ccgctcttgc cagggtgagg
    15181 gttcgctctg gaagcgggac ggtcaagatc aacgaacgct ccgcggaaga ttactttccc
    15241 cgagttatct ggcagtcgga ggcccttgaa gcactgaaga cttccgggct ggaaggcaag
    15301 gtcgacgtct tcgtcaacgc aagcggcgga ggtctcaccg ggcaatccgg agcggtaaga
    15361 atgggcctgg ccagggctct tttaaagatg aatcccgatc tccgtccggc actcaagaaa
    15421 tcgggtcttc tcaccaggga ttccagaatg gtagagagaa agaaattcgg tcagaaaggc
    15481 gctcgcggta agaggcagtt ctccaagcgt taatttgcgg ccgaatatgg cggatagatc
    15541 aggcgcccgg attccgggcg cctttttgtt gacccagggt cttttcgggg atacgataac
    15601 gtcgggatga accctccggg aacgaaggag atgaaaatgg aagatagcca aagtaccagc
    15661 caaaacgccg aaagcagcaa cgatacgagg gtgatcacgg gaccgtaata caaagaggtt
    15721 ccccattttc ccaaaagacc gataccatcc cttcccagtc ctaaaaaacc ccctcgtgag
    15781 taattaacag ttaccgacag agaactgtgc cggaacatgc aggtaatgaa tgcggttttg
    15841 cgtttccgcc tttcttcccg tttgaccggt aaacaggggg tgcagccatg aaggaccgac
    15901 tggacatgct atatcgccag atagattcac ttatcatcga acataaattt acggaggcaa
    15961 aggaaactct ctcgacaatg gaacctgccg acatagggga gatgctcgtt gatgcccccc
    16021 cctccagaag agttcttatc tttcgcatcc tccagaagga tcttgccatt gatgtgttcg
    16081 agtacatgga aggtccagag agagaagaac tcctcgatag ttttaccgaa agagaggcgg
    16141 ccgagatcat agaagaaatg tccgatgacg acaggacggc ccttctggac gaacttcccg
    16201 ccaaaacggt aaaaaaactg atgcagcatc tatctcccga ggaacgcaaa attgccaatc
    16261 gccttctcaa ctaccctccg gattcggccg gaaggattat gacggctgaa tacataggtc
    16321 tgaaggcctc aatgaccgca atggaagctc tccagcatat ccgcagggaa gcaaggagca
    16381 aggagactat atatacctgt tttgtcatgg atagcgagag aaaactcaag ggtgttgtaa
    16441 accttgaaga tatcatcctt gcccaggcgg agaccccggt gtcggacata atggacgatg
    16501 accctgtttt tgtcgcaacc gttacggacc aggaaaaact ggcccagata atttccaggt
    16561 acgatcttca cgtcctgccc gtggtagaca aagagggaag actggtcggt atcgtgactt
    16621 tcgacgacgt actcgacatc cttgaagaag aggccaccga ggactttgaa cgcatggctg
    16681 gtatccagcc cgtggacgac gggtatctcg agacgggtct cctttcgctt gtaaaaaaga
    16741 ggttgccctg gcttgtaata tgtatcctgg cacaggcgat aagtgcctct gtgctcagga
    16801 gatattccct taccatagag agcgtcgtgg ccctgacttt cttcatcccg ctcctgatag
    16861 gtacaggtgg gaacacgggt acacagtcgg ccacccttat tatccggggt atgacccttg
    16921 gggatatatc cgttaaagac ctggggttta tcttttttcg tgagagcatg gctgcaattc
    16981 ttctgggtgt acccctgggt gtgcttgcga taggaagggc gtacatgctg gggaccggtc
    17041 ccgaagttgc tgtggttgtt gcccttgctc ttgtatcggt catcatgatc gggaatcttt
    17101 ccggcgtgct gctccccttc atcgcccggg ccgtcaaaat agacccggca gtgatgtctg
    17161 gacccttcat cacgaccgtg gtggatgtaa tgggtctcct cgtctacttc gagatcgccc
    17221 gcttggtgct gagggtaggc tgaagctcgc gaggtgcgtg agacgtgaaa cgtgaaacgt
    17281 gagacgagaa aattgcggag caagagcctt gtcattctta agagacgtga gggtttgtct
    17341 ttggcttttt cgattcacga ctcacgatta acgactcacg gggcttgccc ctcccgttca
    17401 ccgtttcccg ttcaccgttc acgccgttga ccctgttgtc accctgagga acgaagtgac
    17461 gtgaggatct g
//
LOCUS       DAOTIQ010000039        17305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_3978_length_17305_cov_7.449449, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000039 DAOTIQ010000000
VERSION     DAOTIQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 17305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17305
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_17305_cov_7.449449"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..806
                     /locus_tag="P5541_06170"
     CDS             195..806
                     /locus_tag="P5541_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV71604.1"
                     /translation="MENLKRKGFTLIELLVTIVIVGILAGSMMLLLGSSSASSEATRI
                     VANLRTLKAAADFYFFESGEWPNQSAAGYGIYNIKILSPYLDKTPKNDFADSTLSQAW
                     GTLAKNRFFVYVTGPGSDPNYSTSDYHIYVVANVSDDYADFEVRKKLASMSPGAGIYN
                     GNLSENFGNPSLAVYSSNPSTISAIGNSTSNTPGERFLMMRVR"
     gene            complement(825..1106)
                     /locus_tag="P5541_06175"
     CDS             complement(825..1106)
                     /locus_tag="P5541_06175"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV71605.1"
                     /translation="MKNPDREKISKLEELPNVGRSISADLVSIGICNPGELKGRDPLE
                     LYREVCEKSGQRQDPCLLDVFMSVISFMEGNDPLPWWAFTEDRKKKYQI"
     gene            1180..1707
                     /locus_tag="P5541_06180"
     CDS             1180..1707
                     /locus_tag="P5541_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRV71606.1"
                     /translation="MIDLHYGRQAFDYDCGATALQTVMAYYGVEVRLDELMEALGTDE
                     NGTDVHNMIRTAIRYGFKVEARSAWTLREIKLMIDNGAPPIVLLQAWAEQPKTLKEWE
                     INFNDGHYAILIGHAKGVLLFEDPASFRRTWLREREFMARWHDRDEKTAEVFDHFAMF
                     LLGRDPVLRTPQYMG"
     gene            1812..3560
                     /locus_tag="P5541_06185"
     CDS             1812..3560
                     /locus_tag="P5541_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRV71607.1"
                     /translation="MDKSENFKDPGNFSIPRQRQIDIEASPLSLLVIDRIGIVHLCNG
                     KARDTLGYKGKPERLTRFNDLWDKKLANVPKRILKRLNDDPSTPYSSHLVFKSCEGCP
                     LPCIVDIHSIRLEEPGDHFLVSFQLASEANKGQPQKPDILHPPTPVSKPVATSPRKTE
                     KIKEVISPPKAKGQEEEVNDMDEPEREESIVIMGAFPCGEEIFPKPPDSDKGPGKPAE
                     YGFPPVDLDYPVLDCEGLLANMLPLRASDIHLKTGFPPLFRMGNLKLHAASNMDPLGK
                     NDISRLLMEMMDRETYEKIINGIETDFVFATSNGARFRMNAFLSMGTPSLSIRRINNL
                     IPDIETLGLPPVMKKMAYSKSGLLLVTGPTGSGKSTTLAAIVEEINQNLNVHVITLED
                     PVEFSFSSKKALITQRQIGKDTESFEKALSKVLRQDPDVIMIGEMRDLETISLAVTAA
                     ETGHLVLATLHTRDAPGSIDRIVDVFPAEKQDQIKAELSNTLLGVCSQQLLPRVGGGR
                     ILVTEMLVGTTGVRNRVRVGGTQQLRNTMLTSPEEGMHTFEQSMARLVNSREITLDIA
                     LEHAGDPKELKRFLSS"
     gene            3594..4601
                     /locus_tag="P5541_06190"
     CDS             3594..4601
                     /locus_tag="P5541_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71608.1"
                     /translation="MREENHNRRAPGLLAGTVICIIIMTLVFLGKAQEYSGEYLRGTL
                     DGKNSAYLEKTEDKALSGFLLLSGIKAGLSIIEGSTANLALADIQFGDIVQSVLDAVD
                     ITWKILFATLGILMSMRFLLVLADQSAWFFLSYGAGMIALSFVSGLIILKTSTLPRIF
                     TTFERVSRKAGLFLLMIFTFLYFLIPVSIFGASLLSRSITDTTSVEAMVYIEKYSTEI
                     SGILDTNSENSEKTGEGKNEDSLLKKMIDSPARVINYPRELLSGIKNTIVGQTKALTL
                     KMVSLLVCFLFDVLLFPLIIFVLSYMFLKSLVSSLFGRWNRTDTLEDLQRLSSFLKDR
                     E"
     gene            complement(4646..5551)
                     /locus_tag="P5541_06195"
     CDS             complement(4646..5551)
                     /locus_tag="P5541_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV71609.1"
                     /translation="MMADKLVSNARELTNTEYFYGHRACAGCGGSLALRLAMKVLGPR
                     TFVAIPANCMAAVSFLYPQISFTTNSMITPFPATAAVLSGIEAGARALGLKDYNVVGF
                     AGDGGTADIGIQALSGSIDRRDRVIYVCYDNEAYMNTGIQKSGLTPFGSKTTTTPAGK
                     NIPGSVGRKKDMFEIVAAHGIEYAATASIGYPEDFLKKIGKAAICKTTSYVHVLAPCS
                     TGWGFPVEKTVEMGKDAVDCGLWYLAEYENEKFVLNRKPKEFSPIGDYLKKQARFRHL
                     KEKEIDYIKAGRDKKWKKILENWKG"
     gene            complement(5541..6728)
                     /gene="porA"
                     /locus_tag="P5541_06200"
     CDS             complement(5541..6728)
                     /gene="porA"
                     /locus_tag="P5541_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRV71610.1"
                     /translation="MPGKRIFVSGDEAVAEAVRLAKPHVISAYPITPQTVAVERISDF
                     VEDGTLKSEYLHVESEHTAMSAALGAAAVGARTFTATSSQGLLYMAEVLPYVSGARFP
                     IVMMNANRTLATPWALLADHRDSLSQLDSGWIQVYCENAQETLDMVLQAYRIAEDPRV
                     MAPMMVNLDGFILTHTYEMVDLPDQEEVDAFLPPYITENKMDLDNPRTVCYAAMDDYM
                     EFHVKQELDMRKAPGVIQEVDADFAKRFGRSYGGMVEEYRCDDAEVVLATLGSVTGTA
                     RAVVDLMRDEGRKVGLLKIRFMRPFPREEIAKVAGKVRAMGVLDKDISVGYEGIVFTN
                     VNSSLCGSGKAPAMLNFIGGLGGRNISKGHIREMFEELFRAAEGERCDRIQFINVRCE
                     YDG"
     gene            complement(6718..7029)
                     /locus_tag="P5541_06205"
     CDS             complement(6718..7029)
                     /locus_tag="P5541_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71611.1"
                     /translation="MDRPRIVEYSKPSRVEDYPCGAFKAGHLVQGNAGWRVFRPVIDH
                     EKCVSCLRCFLVCPDGAIDKSGEKLEIDYSFCKGCGVCAHECKVGAISMAREGDESDA
                     G"
     gene            complement(7022..7573)
                     /locus_tag="P5541_06210"
     CDS             complement(7022..7573)
                     /locus_tag="P5541_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV71612.1"
                     /translation="MHEIRWHGRGGQGGFTVARLLGMAASVFEGKYSLAFPSFGPERR
                     GAPVLGFTRICDDPITDRSMMDQCDYAVFLDETLIGPGTLGGIREEGVILVNSVNPEQ
                     YSKMTDRKIVAFDATTLALEILGRPITNTSMLGALVAVSGVVSLDSVKEAIRQGMNPS
                     LVEKNCVVVEKACGMVREVITNG"
     gene            complement(7602..8237)
                     /locus_tag="P5541_06215"
     CDS             complement(7602..8237)
                     /locus_tag="P5541_06215"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71613.1"
                     /translation="MKAHKEEIYRVLRNRIIGLDYKPGEVLSDRDLVAGFGTSRTPVR
                     EAILKLQKDGLVETVPRVGTFVASIDIKSVRHAYEMKKNLEGLAAELAASRAGEEQIR
                     GLLDLAGAFAGLDNFRDYRECIEKDSRFHELTHEASGNPLLMKTLENLSLITVRFLQH
                     IQYVENEYEWYKDSINAIAEAIRRRDPAKARKEAEQHTAAFLTKLASYFFG"
     gene            complement(8329..9384)
                     /locus_tag="P5541_06220"
     CDS             complement(8329..9384)
                     /locus_tag="P5541_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRV71614.1"
                     /translation="MRARGSRAFTLVELLIVLVIIGIFAGSLMLVMGSGADRSKATRV
                     ISDMAVLKRAAMVRFLDSGSMPSDPEELRPYVAKYHLAGPDGIPYGISTGESGSGIAA
                     NLENAPAGVRDQLARSASRVALYGDPTFTEYYSGGNVAYTPFSRIGTPTGDGNPSFSP
                     GEADFSRIYNAALNETRWITTDGGLTAEGSGRMLFADQEWGDFEMKIDTQFLALAPNP
                     GFAVYYRATDNGGNLPQAGYSFQFEPRYQLPDGSWTGTGYFRVRKYENGNESTQPATH
                     VRITDILGPGYDMNANYQVTIRAEGERHQVFISDGSTEYKVFDYTDAGPSSGQSGLRL
                     WSNTEVSLQNIEITPLD"
     gene            complement(9493..10476)
                     /locus_tag="P5541_06225"
     CDS             complement(9493..10476)
                     /locus_tag="P5541_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRV71615.1"
                     /translation="MEIMLLSLREIESLNIHVSEVMKVVERGFALKGEEKMEMPAKIG
                     IHPRKDCFIHAMPCYVGGDVDAAGIKWVAGYPINQARGLPYITGIMCLNESETGFAKA
                     IMDANWITAWRTGAATGICARHMADPSSETIAVIGLGVQGRTNTMALAEALPKLKTVK
                     IYDAFEAQVAKYTSLVEPDLKGIDVVPCKSVEETVKDADVVVTCTPIVADPERFVKSK
                     WLKEDVLAVAVDYDSAFDADVMTGATAFVCDDMNQYLWTQEHGVYFQKGYPTESQILG
                     DMGHLCAGKKKVERKGRRGAVLMGIASHDIMTANLIFDQAKARGVGRMVEI"
     gene            10606..11862
                     /locus_tag="P5541_06230"
     CDS             10606..11862
                     /locus_tag="P5541_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV71616.1"
                     /translation="MAVFDVVVIGGGPAGMIAAGRAAEVGASVALLEKNPELGKKLLI
                     TGKGRCNFAHNEEDPLKIAEAFGRNGRFLISALNRFGLYETLDFFRSRGVEPVAERGN
                     RFFPAAGQNAYTVRSALMEYLTEKDVTIMTGTSVRSLEGKNGEINHAVTAGSRKVTGR
                     NFIVSTGGLSYPSTGSTGDGMQWARDMGHNITPTEPSICPVEAKESFCKELQGLSLRN
                     VSVSVWQNGKKTDERSGEMLFTHFGVSGPIIMDLSRTISLLLKKGPVTLFLDMKPALD
                     ETKLDGRLLRDFSKLAGKEISTCVRGLVPRAMVPVILKLAGIHEDKSIDAITREERRN
                     LVEALKNISITPVALLGYRWAIVTAGGVSLKDVDPKTMASKKLENLFFAGEVLDLDAP
                     TGGFNLQACWTTGYVAGEEAARKALS"
     gene            complement(11924..12340)
                     /locus_tag="P5541_06235"
     CDS             complement(11924..12340)
                     /locus_tag="P5541_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71617.1"
                     /translation="MRKLSCLLCVLFFVVVAIPAAASVIDLSGPWILEFPQGQGLAVL
                     QPTGGDPTSYTGQVTIPHPAYSSGKLTFNVKMLTAPSYVVPGNIITFQPTTVANIGFF
                     LMNLSSSSNGIAWIMHNAGSENYIINLANERAPAHR"
     gene            12435..14159
                     /gene="ade"
                     /locus_tag="P5541_06240"
     CDS             12435..14159
                     /gene="ade"
                     /locus_tag="P5541_06240"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRV71618.1"
                     /translation="MSVPSLLAVSRGDIPAELVLKGALVANVLSMELEEADVAIHEGR
                     IAGVGKGYEGKQNLDLSGMTLAPGFFDAHCHIESTMLVPSSFAGLVAARGTSAVAADP
                     HEIANTCGMAGIEYMFLDAMNCPVDFFYTAPSCVPASSFETPLEPLGAEMVSEMFSRG
                     WCHSLGEVMNFPAVIAGGEDIWRKITAASGRVKNGHAPGLTGRNLCAYLVSGCDSDHE
                     CTLASEALEKLRRGMWLMIREGATEKNLEELAPLIREDERRASRCMLVSDDLTASYIR
                     DRGHMDEKVRLAVACGISPLSALRMVTLSPATYFGLRDRGAIAPGYLADIVAVDDIES
                     CRVRRVWKNGRSVATYGNLERPCGGTEITDAIRVSGADLTSPKPEDLGIPVKSGNRIR
                     VIGVLPGQVITEHRVMEPRIISGKATADPSRDVALMAVMGKNRGTGRLSQGFVQGLGL
                     KEGAIGSSVAHDAHNFVVAGMDELSISTAFRKLVSSGGGLVAARGTEVTAFLSLPAGG
                     LMNPGRPDEVIESLKNLEMAASELGTTIENPFMTMSFLCLSVIPELKLTDSGYVDFSR
                     GGAQEILA"
     gene            complement(14211..14780)
                     /locus_tag="P5541_06245"
     CDS             complement(14211..14780)
                     /locus_tag="P5541_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRV71619.1"
                     /translation="MIKPFIALMLGSSLAMDALAVSVAMVLCARVAVRVSSVTRVAGA
                     FGLFQALMPMAGWVLAERSMSVIGEIDHWIAFALLAFVGGKMVFDGLRNGEECPFSND
                     PSRGVPLLMLSLGTSIDAMAVGVSFDPLGLAPFFTSVVIGFVTFGIVSAGMVLANRIG
                     KSFGEKMTVAGGLILIAIGGRILVTHIGG"
     gene            complement(14777..15442)
                     /locus_tag="P5541_06250"
     CDS             complement(14777..15442)
                     /locus_tag="P5541_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71620.1"
                     /translation="MGILPYWDQNVDLEISPWAPYGKDFNPISVNSMHSDQLGAIASL
                     ALGMDSELMFLESSVTGQVGALFRENLMMPGRRRGQDGGFSVMFGYSQVMEALLGMFA
                     QAYPGLRAGLAVCGVWDRSMLVDERKVIVLVGETLFLNEAGCLNALQQVMLAASTDSG
                     SSSFFAEQFGYRRFSIPFETDDVTNLAILKLLFDFEFEATRAVVGPPPEVSLPVLFKE
                     SPL"
     gene            complement(15490..16305)
                     /locus_tag="P5541_06255"
     CDS             complement(15490..16305)
                     /locus_tag="P5541_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRV71621.1"
                     /translation="MPDLCEERIQFLADHFEEMYHRYNRPEFIDPDPVGVVRSYPDLP
                     DREVAAVVSSCLAYGRAAGIVKSARAILDPMGPSPHGFLTGSSPLEIRMICQNFRHRF
                     TDCNDIYELLLSIRDILFEWGSIEALFASGLGKDEKVTGGLRKLYRSLQKNSGKRKNS
                     LLPNVSRGSACKRYNLMIKWMVRNDGIDPGGWRCLSPASLILPLDTHMIRICQELGLV
                     TRKTPDMVTANQATRSFRLIAPQDPTKYDFTLTRFGIRPDLKMSDLLGQCGHS"
     gene            complement(16298..>17305)
                     /locus_tag="P5541_06260"
     CDS             complement(16298..>17305)
                     /locus_tag="P5541_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRV71622.1"
                     /translation="VLPSGDLDYLVLNHAEMDHTGSLPSVMRELRPEKVICSGACMDA
                     IIEHFGKQDWPFHVVKTGDSLSLGKRTVRFIETKMLHWPDSMFSYLEEDHLLVSSDAF
                     GQHYATGERFDDEADQSELMKQAAKYYANIILPYSGLVLKLLENVKAMGIKIEMIAPD
                     HGLIWRKKVPDILQSYTDWASGKVKAKAVVAYDTMWGSTAKMAHAVIDGLISRGVEVK
                     ATDLKGWHRSDVMTELLDSAALVLGSPTLNNGYLPRMADLLCYARGLRPMCRVGASFG
                     SYGWGGESVKLLNEELGRMKIDLAHEGLKIKYVPSPGDLEECREMGISVGRKIMELPG
                     A"
BASE COUNT         4128 a         4730 c         4293 g         4154 t
ORIGIN      
        1 tcccaaagtc ccccggctcc ttctgcattg tatcaccgct ttttacgggt gcgtcttttc
       61 aaatccggat ttcccttgta tccttcgaat ggtttgtata ttatcctgct atgcagaaaa
      121 tttcctttta ttgatatgaa gcagcgggga cacttggaac accagcctga ccggcggcgg
      181 atagggaggt aaccgtggag aatctgaaaa ggaagggttt cacccttatc gaactgctgg
      241 taacgatagt catcgtcggt atcctggccg gatcaatgat gcttttactg gggagttcct
      301 ccgcatcgtc ggaggcaacc cgtattgttg ccaacctgcg taccctcaag gccgcggccg
      361 acttctattt ttttgagagc ggagagtggc caaaccagtc cgccgccggt tatgggatat
      421 acaacataaa gattctctct ccctatctcg acaaaacccc aaaaaacgat ttcgctgaca
      481 gtactttaag tcaagcatgg ggaactcttg cgaaaaaccg tttttttgtc tatgtcacag
      541 ggccgggatc agatccaaac tactctacct ctgactatca catctatgta gtagcaaatg
      601 tcagtgatga ttacgcggat tttgaggtca ggaagaagct ggcctcgatg tcaccgggag
      661 ccggaattta caacggcaac ctcagtgaaa atttcggcaa tccttctctt gctgtttatt
      721 cctctaaccc ctcgacaatt tctgccatcg gtaacagcac ttcaaacact ccgggggaga
      781 gattcctgat gatgcgggtc aggtaggatc ccgaccagcc cgatttatat ctggtatttt
      841 ttctttctat cttcggtgaa agcccaccag ggtagagggt catttccttc cataaaactt
      901 ataacggaca tgaatacgtc gagaagacag gggtcctgcc tctgtccgga cttctcgcat
      961 acctctctgt aaagttccag gggatcgcgc cccttcagtt ccccggggtt gcaaatacct
     1021 atcgaaacca ggtccgccga gatagatctg cccacgttcg gcagttcctc aagtttcgat
     1081 atcttttccc tgtccgggtt cttcattttc ctgccctccc tgtatctgcc aggtggacct
     1141 ataatatcaa ttattcctta cctgagagga ggctactcct tgatcgacct tcattacggc
     1201 agacaggctt tcgattacga ttgtggagcc acagcccttc agacggtaat ggcttactac
     1261 ggagtggaag ttcgcctgga cgaactgatg gaagccctcg gaactgacga gaacggaacc
     1321 gacgtacaca acatgatacg caccgccata aggtacggct tcaaggttga agcacgctcc
     1381 gcctggacgt taagggaaat aaaattaatg attgataatg gtgccccccc aatagtgctt
     1441 ctccaggcat gggcggagca accgaagacc ctcaaggagt gggagataaa tttcaacgac
     1501 ggccactacg ctattcttat aggccacgcc aagggggttc ttctctttga agacccggcg
     1561 tcattcagga ggacatggct gagagaacgc gaatttatgg cgaggtggca cgaccgggat
     1621 gaaaaaaccg ccgaagtttt tgatcatttc gccatgtttc tccttggcag ggatccggtc
     1681 ctccggacac ctcagtatat ggggtaggta gcgttaattg ctacctggta ttccttcgtg
     1741 tataaaagga gtgaaggaat ttagacaacg ttatctcttt tcccgacagc aatagcccgg
     1801 aggagatcac cttggacaaa agtgaaaatt tcaaggatcc tggcaacttc tccatcccca
     1861 gacagagaca gatagacatt gaagcaagtc cgctttcgtt gcttgtcatc gaccggatcg
     1921 ggatcgtcca tctctgcaac gggaaggctc gcgacaccct cggttacaag ggaaaaccgg
     1981 aaagactgac aaggtttaac gacctttggg acaaaaaact tgccaatgtt cccaaaagga
     2041 tcctgaagcg ccttaacgat gacccttcaa ccccttacag cagccacctg gtcttcaaaa
     2101 gctgcgaagg atgccccctg ccgtgcattg tcgacattca ctccatacgc cttgaggaac
     2161 ctggcgacca tttcctggta tctttccagt tggcttcgga ggccaataag ggccaacccc
     2221 aaaaaccgga tatccttcac cccccaacgc cggtgtccaa accagtcgct acttcaccgc
     2281 gaaaaacaga gaaaataaag gaagtcatat ctccgcccaa ggcgaaaggc caggaggaag
     2341 aggtgaatga tatggacgaa ccagaaaggg aagaatctat agtcattatg ggtgcgttcc
     2401 cttgcggcga agagattttt ccaaagccgc ctgactctga caaaggcccc ggaaaaccgg
     2461 cagagtacgg gttcccccct gttgatctgg attacccggt ccttgattgt gagggcttac
     2521 tggcaaacat gttacccctc agggcaagcg atattcacct taaaacaggc tttcccccgc
     2581 ttttccggat ggggaacctt aaactgcatg ccgcctcaaa tatggatccc ctgggcaaga
     2641 atgatatttc ccgactgctt atggagatga tggatcggga aacctacgag aaaataatca
     2701 acggtatcga gacagatttt gtttttgcca ccagcaacgg cgcaaggttc cggatgaatg
     2761 cctttctctc catgggaacg ccttcgctga gtatccggag gatcaacaac ctgatacctg
     2821 atatcgaaac cctcgggcta cctccggtca tgaaaaaaat ggcatactcc aaaagcggcc
     2881 tgctcctggt cacagggccc accggcagcg gtaaatccac gacccttgcc gcgattgtag
     2941 aagagatcaa tcagaacctg aacgttcacg ttatcaccct tgaggatcct gtcgagttct
     3001 ccttctcttc gaagaaagcc cttataaccc agcggcagat agggaaggac acggaatctt
     3061 tcgaaaaagc attgtcaaag gtcctgcgac aggatccgga tgttatcatg atcggcgaaa
     3121 tgagggatct cgagaccatc tctctcgcag ttaccgccgc agaaacggga cacctggttc
     3181 ttgccactct ccatacaagg gacgctcccg gctcgatcga cagaatagtc gatgttttcc
     3241 ctgccgagaa gcaggaccag atcaaggccg aactatccaa cacccttctg ggagtatgct
     3301 ctcaacaact tcttccccgg gtcggcggag ggaggatcct ggttacggag atgctcgttg
     3361 ggacaacagg agtcaggaac agggttcgcg tcggggggac ccagcagtta cgtaacacga
     3421 tgctcacaag tccggaagaa ggcatgcaca ctttcgaaca gagcatggcc agactggtca
     3481 acagcaggga gataactctt gatattgccc ttgaacatgc cggggaccca aaggaactaa
     3541 agaggttcct ttcttcctga atcatcctga tgaaataaca ggtggtaaaa atattgagag
     3601 aagaaaacca taatcgcagg gcccctggcc tgctggccgg gacagttatc tgcatcatta
     3661 tcatgacact ggtgtttctc ggcaaagccc aggaatattc cggtgaatat ttaaggggaa
     3721 cccttgacgg gaaaaactct gcctacctgg aaaagaccga ggacaaggcc ctttccggat
     3781 ttcttctact ctcggggatc aaggccggac tatccataat cgaggggagc accgcaaatc
     3841 tggcacttgc cgatatccag tttggagata ttgtccaatc ggtcctggac gccgtcgata
     3901 taacatggaa gatccttttc gcaacccttg gaatactgat gtccatgagg ttccttctgg
     3961 ttctggcgga ccagtcggca tggtttttcc tgtcttacgg ggcaggaatg atcgccctgt
     4021 cttttgtgtc aggattgatc atcctgaaga catcgactct tccccgcatt ttcaccacct
     4081 ttgagagggt ttccagaaaa gcagggctct tcctgttaat gattttcacc tttctttatt
     4141 tccttatccc tgtttccata ttcggggcat ccctgctctc caggtcaata acagacacca
     4201 cttccgtcga ggccatggtt tacatagaaa aatacagtac tgaaatttcg ggcatcctgg
     4261 ataccaatag cgaaaatagc gaaaaaacgg gagaaggaaa aaacgaagac agtcttctta
     4321 aaaaaatgat cgattctccc gccagagtga tcaattatcc ccgggagttg ctttcgggga
     4381 taaaaaacac aatagtagga cagaccaagg ctctgaccct caagatggtc agccttcttg
     4441 tctgctttct tttcgatgtg cttcttttcc cgctgatcat ctttgtcctt tcgtacatgt
     4501 tcctgaaaag ccttgtatcg agccttttcg gaaggtggaa ccgcaccgat acccttgaag
     4561 atctgcaaag gctatcctcg tttttaaaag accgtgaata gccataaaaa tcgggggcac
     4621 cccaaaaggc acccccgatt tttttttatc ctttccagtt ctccagaatc tttttccact
     4681 tcttgtctct tccggccttt atgtaatcta tctctttctc tttgagatgc ctgaatctgg
     4741 cctgtttttt gaggtaatcc ccgatgggag aaaattcctt cggcttcctg ttgaggacga
     4801 acttttcgtt ctcgtattcg gccaggtacc agagtccgca atccaccgcg tctttaccca
     4861 tctccacggt cttctcaacc gggaaacccc atcccgtcga gcatggagca agaacatgga
     4921 cataggacgt ggttttgcat attgccgcct tgccgatttt tttcaaaaaa tcctcagggt
     4981 agcctatgct tgctgtcgcg gcatattcga tgccgtgggc agccactatc tcgaacatgt
     5041 ccttcttgcg acccaccgag ccggggatgt tcttcccggc cggagtggta gtggtctttg
     5101 atccgaaggg agtcagcccg ctcttctgta tccctgtgtt catgtaggcc tcattatcgt
     5161 agcagacata aataaccctg tctctcctgt caatggaacc ggaaagagcc tgtatgccga
     5221 tatcagcagt ccctccgtca ccggcaaagc cgacaacatt gtagtcctta aggcccagcg
     5281 cccgggcccc ggcctcgatc ccggagagca ccgcggccgt agcggggaaa ggcgtgatca
     5341 ttgaattggt cgtaaaggat atctggggat aaaggaaact gaccgctgcc atgcagtttg
     5401 ccggtatggc aacaaaggtc cttgggccca aaaccttcat ggcaagtctt agcgcaaggc
     5461 tgccgccgca tccggcgcac gccctgtgcc catagaaata ttccgtgttg gtgagttccc
     5521 tggcgttgga gaccagttta tcagccatca tattcacacc tcacgtttat gaactggatc
     5581 ctatcgcatc tctctccttc tgctgcccgg aaaagttcct cgaacatttc cctgatatgg
     5641 cccttggaga tgtttctgcc tccaagtcca ccgatgaaat tcagcattgc cggggctttg
     5701 cctgaaccgc aaagggatga attcacatta gtgaagacta tcccttcata tcccacagaa
     5761 atatccttgt ccagaacacc catggcacga acctttccgg caaccttcgc tatctcttcc
     5821 ctggggaatg gacgcatgaa gcggatcttt aacagcccga ccttgcggcc ctcgtccctc
     5881 atcaggtcga caaccgccct ggccgtcccc gtaacgcttc caagggtcgc aagaaccacc
     5941 tcggcatcat cgcagcggta ttcttcgacc atccctccat aactgcgtcc aaatcttttt
     6001 gcgaaatcag cgtcaacctc ctggatgacc cccggagcct tacgcatgtc aagttcctgc
     6061 tttacatgaa actccatgta atcatccatc gcggcataac agacggtcct ggggttgtcc
     6121 aggtccattt tgttttcggt aatgtagggt ggcaggaaag cgtcaacttc ttcctgatcg
     6181 ggcaggtcca ccatttcgta ggtgtgagta agtatgaacc cgtccaggtt gaccatcatg
     6241 ggcgccatta cccgcggatc ttccgctatg cggtaagcct gcaggaccat gtcgagagtc
     6301 tcctgggcat tctcgcaata gacctgtatc cagccactgt caagttggga aagggaatcc
     6361 ctgtgatccg caagcaatgc ccacggcgta gcaagagtgc ggttggcgtt catcattact
     6421 atggggaacc tggctccgct gacataagga agaacctccg ccatgtagag cagcccctga
     6481 gaggatgttg ccgtaaaggt ccttgctccc acggctgctg ctccaagtgc cgccgacatc
     6541 gccgtgtgct ccgactctac gtggagatac tcggacttga gggtcccatc ctcgacaaaa
     6601 tcggagatcc tctccaccgc aacggtctgt ggggttatcg ggtaggctga tatcacatga
     6661 ggttttgcga gccggactgc ctccgccaca gcctcgtcgc cggaaacaaa tatcctttta
     6721 cccggcatcg ctctcatctc cttccctggc catgcttatg gccccgacct tgcactcgtg
     6781 ggcacatacg ccgcatccct tgcagaaaga gtagtcaatc tccagttttt ctcccgattt
     6841 gtctatcgcc ccatccgggc aaacaagaaa acaccttaga caggacacac atttttcgtg
     6901 gtctattacc ggcctgaaga ctctccatcc cgcgttgccc tggaccaggt gtccggcctt
     6961 gaaagctccg caagggtaat cttcaaccct tgatggttta ctgtattcaa ctatcctggg
     7021 cctatccatt ggttattacc tccctgacca ttccacaggc cttctccact accacgcagt
     7081 tcttttctac aagggaagga ttcatcccct gccttatggc ttccttgaca gaatccagag
     7141 aaacaactcc ggaaaccgcc acgagagccc cgagcatcga cgtattggtg attggccttc
     7201 ccaggatctc cagggcaagt gttgtggcgt caaaggccac gatctttctg tcggtcatct
     7261 tactgtactg ttcgggatta acgctgttga ccaggatgac cccttcctcc ctgatacctc
     7321 ccagagtgcc ggggccaata agagtctcat cgaggaacac ggcataatcg cactggtcca
     7381 tcatgcttct gtcagtgata gggtcatcgc agatcctggt gaaacccagg accggggctc
     7441 cccttctctc cggcccaaag gacggaaaag ctagagagta cttcccctcg aatactgatg
     7501 ccgccatccc gagaagccgg gctactgtaa aaccgccctg acctcctctt ccgtgccatc
     7561 ttatctcgtg caattgattt accccctctt gtctctcatt tctagccaaa gaaataactc
     7621 gccagtttcg tcagaaacgc ggcagtgtgc tgttccgcct cctttcgagc ttttgcagga
     7681 tctcttctcc tgatagcctc ggctatggcg ttaattgaat ccttgtacca ttcgtattcg
     7741 ttctcaacgt actggatatg ctgaagaaaa cggactgtta ttaggctgag gttttcgagg
     7801 gtcttcataa gcagagggtt accggaggct tcatgggtaa gctcgtggaa cctgctgtct
     7861 ttctcgatgc attccctgta gtcacgaaag ttatccaggc ccgcaaaggc tccggccagg
     7921 tcaagaaggc cccggatctg ctcctctccc gctcttgatg cggccagttc ggcagccaga
     7981 ccctccaggt ttttcttcat ctcataggca tgcctgacag actttatgtc tatggaggca
     8041 acaaaagttc caactctcgg cactgtttcc acgagcccgt ctttctgaag tttaaggatg
     8101 gcttccctca cgggagtacg acttgttccg aatcccgcga caaggtccct gtcggagagg
     8161 acctcaccgg gcttgtaatc caggcctatt atcctgttcc tgagaacgcg gtatatctct
     8221 tccttgtgag ctttcattat gccgcctctg atatgggttt attatacctc tagtatatca
     8281 gttgttgctc aacaggcata tcctttgatg caaacattcc cttgaggatc aatcgagtgg
     8341 ggttatttct atgttctgga gggatacctc ggtatttgac caaaggcgca gtccgctctg
     8401 accggatgac ggccccgcgt cggtataatc gaagaccttg tattctgtgg aaccgtcgct
     8461 gataaagacc tggtgtcttt ctccttcggc tcttattgtc acctggtaat tggcattcat
     8521 atcatagccg gggccgagaa tatcggtaat cctgacgtgg gtcgctggct gtgtcgactc
     8581 gttaccgttt tcatactttc gcacgcggaa ataacctgtc ccggtccagc ttccatcagg
     8641 gagttgatat cttggctcga actggaaaga gtaacctgcc tggggtaaat tgcctccgtt
     8701 atcagtggcc ctgtagtaaa ccgcaaatcc cgggttggga gcaagagcca ggaattgcgt
     8761 atcgatcttc atttcgaaat ctccccattc ctgatctgca aaaagcattc ggccagaccc
     8821 ttccgcggtc agtccgccat cagtcgttat ccaccgggtc tcattcaggg ccgcgttgta
     8881 tattctgctg aaatccgctt ctccgggcga aaatgacgga ttaccgtccc cagttggcgt
     8941 ccctatcctg gagaaaggag tataggctac atttccgcca ctataatact cagtaaaggt
     9001 agggtcgccg taaagggcaa ccctggaggc cgatcttgcc agttgatccc tgacacctgc
     9061 gggagcattc tcgagatttg cggctatccc gcttccgctt tctccggtgg aaattccgta
     9121 aggaattccg tcgggtccgg caaggtggta ttttgccacg tagggcctca gttcctccgg
     9181 atctgagggc atggatccgc tgtccaagaa acgcaccatg gcggcccgtt tgagaactgc
     9241 catatcgctg atcaccctcg tggcctttga cctgtcggcc ccggaaccca tgacaagcat
     9301 aagggacccg gcaaatatgc cgatgattac caatactatc agcagttcaa caagggtgaa
     9361 ggctctcgat cctcgggctc tcaaggcata cacctccctc accagaatat tagcataatt
     9421 acagtttatc ttgtaaccag acataatcag gggccctgcc ggaaaggacc cctgactggt
     9481 ttgatcagga atttatatct ccaccatcct gcccacgcct cttgcctttg cctgatcgaa
     9541 gatcaggttc gccgtcatga tgtcgtggct tgcgataccc atcaggacag ctccccgtct
     9601 cccctttcgt tcgacttttt tctttcccgc gcaaaggtgc cccatgtcgc ccagtatctg
     9661 agattcagtg ggataaccct tctggaagta gacaccgtgc tcctgggtcc acaagtactg
     9721 gttcatgtcg tcgcagacga acgctgtcgc tccggtcatg acatcggcat cgaaggcaga
     9781 gtcgtaatcg acagctacgg ccagaacatc ttctttaagc catttcgatt ttacgaatct
     9841 ctctggatcg gcaacgatcg gagtacaggt aaccactaca tcggcatctt tcaccgtttc
     9901 ttcgacactt ttacagggaa cgacatcaat acccttcagg tcgggttcaa ctaggctagt
     9961 gtattttgca acctgggcct cgaacgcatc gtatattttt acggtcttca gttttgggag
    10021 cgcctcggca agagccatgg tgtttgttcg tccctgcaca ccgaggccaa taacagctat
    10081 cgtttcggaa gaagggtccg ccatgtgtct tgcgcagatg ccggtggccg cccccgttcg
    10141 ccatgccgtt atccagttcg catccatgat cgccttggca aaaccagttt cggattcgtt
    10201 gagacacatg atgccagtga tgtacggaag ccccctggcc tgatttatgg ggtatcccgc
    10261 cacccacttg ataccggcag catccacatc gccgccgaca taacatggca tggcgtgaat
    10321 gaagcaatcc ttgcggggat gaatgccgat ctttgccggc atctccatct tctcttcacc
    10381 tttaagcgca aatcctctct cgaccacttt cattacttcc gatacgtgaa tattgagaga
    10441 ttcgatctct ctgagggaca acagcatgat ttccatccac tccgcctcct gtccttattg
    10501 atatgccgat cacttttcct gtccagatgt ttttcataaa atcactcttg attatagttt
    10561 cctgttatca tgtcgaaaag cccgaataag ggaggaaagc catttatggc tgttttcgat
    10621 gtagtggtta taggcggtgg cccggccgga atgatcgcag cgggacgtgc ggcggaagtc
    10681 ggagcctcgg tcgcgctgct ggaaaagaac cctgaactgg ggaaaaaact gcttatcacg
    10741 ggcaagggca gatgcaattt cgctcacaac gaggaggatc ccctcaagat agccgaagca
    10801 ttcggtcgaa atggccgttt tctgatctcg gcactgaacc ggttcggcct ttatgaaact
    10861 ctggactttt ttcgcagccg cggggtagaa cccgtcgccg agcgcggaaa caggtttttc
    10921 cccgctgccg ggcagaacgc ctataccgtc aggtctgctt tgatggaata cttgacggag
    10981 aaggatgtaa cgataatgac gggtacatca gtccgatccc tggaagggaa gaacggggag
    11041 atcaaccatg ccgtaacggc cggttcccgg aaggtgaccg gaaggaattt catcgtaagc
    11101 accggtgggc tatcttaccc ttcgaccggg tccacagggg acggcatgca gtgggcccgg
    11161 gacatgggac acaacatcac ccccactgaa ccttcgatat gtcccgtgga ggccaaagag
    11221 agtttttgca aggaactcca gggcctgagc cttcgaaatg tttcagtttc cgtctggcag
    11281 aacgggaaaa aaacggatga aaggtcgggc gagatgctct tcacacactt cggcgtcagc
    11341 ggaccgataa taatggacct atccaggacg atatcccttc ttctgaaaaa aggcccggtt
    11401 actcttttcc tcgacatgaa acccgccctg gacgaaacga aactggatgg acgccttctc
    11461 cgggatttca gcaaacttgc cggtaaagag atcagcactt gcgtcagggg gcttgtacca
    11521 agggcaatgg taccggtcat ccttaaactg gctggtattc atgaagacaa aagcattgat
    11581 gccataaccc gggaagagcg acgaaacctt gtcgaggcat tgaagaacat atccattacc
    11641 cctgtggctc tcctgggtta ccgctgggcg atcgttaccg cgggcggggt ctctttgaag
    11701 gacgtggacc caaagacaat ggcttccaaa aagttggaga accttttctt cgctggagag
    11761 gtcctggatc tcgacgcccc aacgggcgga ttcaacctgc aggcctgctg gacaacggga
    11821 tacgtagccg gggaagaagc cgccaggaag gctctctcat gaagcaggca ggctctcacc
    11881 cagggtgaga gcctgccatg gaaacatatg tgtttcgcgg aacctacctg tgtgccggcg
    11941 ctctttcatt ggccaggttg ataatatagt tctctgatcc ggcgttgtgc attatccagg
    12001 cgataccgtt actggaactg ctaagattca tcaggaagaa cccgatgttc gccacggttg
    12061 ttggctggaa agttataatg ttccccggta cgacataact cggcgcggta agcatcttga
    12121 cattgaaggt tagttttcca ctggaatagg ccgggtgagg gattgtgacc tgccccgtgt
    12181 agctcgtggg atcgccccct gtcggctgaa gaacggcaag tccctgccct tgcgggaatt
    12241 caagtatcca ggggcccgac aagtcaataa cgctcgcagc ggccggaatc gcgacaacca
    12301 caaaaaacag cacgcataac aggcaggaaa gttttctcat tattacttcc cctttctcgg
    12361 catgacactg gttttatgac attttaaagt atcaaaagtc cgacagtcaa ctactgaaac
    12421 agggagatga tcatatgtcc gtaccttcgc tgcttgcggt ctcaaggggc gatataccgg
    12481 ctgaactcgt cctgaaagga gcactcgtag caaatgtcct gtcaatggaa cttgaagagg
    12541 ccgacgtggc tattcacgaa ggacgtattg caggggtcgg caagggttac gaaggaaaac
    12601 agaacctgga cctttcggga atgacccttg ctccaggctt tttcgatgct cactgccaca
    12661 tcgagagcac catgcttgtc ccatcctcct ttgcgggact ggtggcggca agaggaactt
    12721 ccgccgtagc ggctgaccca cacgaaatag ccaacacctg cggtatggcc gggatcgaat
    12781 acatgttcct cgacgctatg aattgtcccg ttgatttctt ttataccgcc ccttcgtgtg
    12841 tgccagcttc gtctttcgag acccccctgg aacctctcgg agcagaaatg gtgtccgaga
    12901 tgttctcgag aggctggtgc cattctctcg gcgaggtaat gaacttcccg gccgttatag
    12961 caggcgggga ggatatctgg cgaaagataa cggcagcttc aggcagagta aagaatggcc
    13021 acgcccccgg gctgacgggc cggaaccttt gtgcctatct tgtcagcggc tgtgattcgg
    13081 accacgagtg cacccttgca agcgaggccc tcgaaaagct ccggaggggc atgtggctta
    13141 tgatacgaga gggagcaact gaaaaaaacc tggaggaact ggcccctctg atcagggaag
    13201 atgaaagaag ggcatcccgg tgtatgctgg taagtgatga tcttaccgcc tcctatattc
    13261 gtgacagggg acacatggac gagaaagtcc ggctggcggt tgcgtgcggt atatcccctc
    13321 tttccgccct tcgtatggta accctgtctc cggcaaccta tttcgggctt cgcgaccggg
    13381 gagcaattgc tccgggatac cttgccgata tagtggcagt ggatgacatt gaatcgtgcc
    13441 gggtgaggag ggtctggaaa aacggaaggt ctgtagcaac atacggcaac ctggaaagac
    13501 cctgcggcgg aacggagata acggatgcaa taagggtttc cggagcagat ctgaccagcc
    13561 ccaagccgga agatctggga atccccgtga aaagcggcaa caggataagg gttatcggcg
    13621 ttcttcccgg tcaggtaata acggaacaca gggtcatgga acccaggata atctcaggca
    13681 aggcgacggc agacccgtca agagacgtcg ctcttatggc cgtaatggga aaaaacaggg
    13741 gaacaggaag gctttcacaa ggttttgtcc aagggttggg tctgaaggaa ggggcaatcg
    13801 gttcttctgt ggctcatgac gctcacaatt tcgtcgtagc ggggatggac gaattgtcca
    13861 taagcactgc tttcagaaaa ctcgtttcct caggtggggg actggtcgcc gcaagaggta
    13921 cggaggtaac ggccttcctt tcgcttcccg ccggggggct gatgaacccg ggcagaccag
    13981 acgaagttat tgaaagcctg aaaaaccttg aaatggcagc atccgaactt ggaacaacca
    14041 ttgaaaaccc tttcatgaca atgagctttc tgtgcctttc ggttatcccg gaactcaaac
    14101 tgaccgattc gggttacgtc gatttttccc gggggggagc ccaggaaata ctggcctgaa
    14161 taattatcca gaaaaatttt ttcgcgggac tggggcttgt cctccgtccc ctagccccca
    14221 atgtgggtca ctagtatcct gccccctata gctatcagta taagaccacc tgctactgtc
    14281 attttttctc caaaactttt ccctatgcgg ttcgcaagga ccatcccggc cgaaacgatc
    14341 ccgaaggtga caaagcctat tacgacagac gtaaaaaaag gtgccagtcc cagaggatca
    14401 aaacttaccc ccaccgccat cgcgtcaatg cttgttccca gagacagcat caacaggggg
    14461 accccgcgtg aaggatcgtt gctgaaagga cattcttccc cgttcctgag accatcgaag
    14521 accattttgc cccccacaaa ggccagcagc gcaaaagcta tccagtgatc tatctcccca
    14581 atgacagaca ttgatctttc tgccagtacc catccggcca tcggcatcag ggcctggaaa
    14641 aggccgaaag ccccagccac ccttgtgaca gatgaaaccc tgacagcgac acgggcacac
    14701 aaaaccattg ccacggaaac agccagagca tccatggcaa gactggatcc caacattaac
    14761 gcaataaagg gtttgatcaa aggggagact ccttgaacag gaccggaagg gaaacctccg
    14821 gagggggacc caccactgcc cttgtagctt cgaactcaaa atcgaaaagc agtttgagaa
    14881 tcgccagatt ggtaacgtca tcggtctcga agggtatgga gaatcttcga tatccgaact
    14941 gctccgcaaa aaatgagctg gaaccgctgt cagttgaggc cgccagcatt acctgctgaa
    15001 gtgcgttcag gcaccctgcc tcgttgagga acaaagtctc tccaacaagg actatgacct
    15061 tgcgctcatc aaccagcatg gatctgtccc atacaccaca tacggcaaga ccggccctga
    15121 ggccgggata agcctgggca aacattccaa gaagggcttc cataacctgg gagtatccga
    15181 acatgacaga gaatccgcca tcctggccac ggcgtctccc tggcatcatg aggttttccc
    15241 gaaaaagggc acctacctgc cctgttacgg aactttccag gaacatgagt tcgctgtcca
    15301 ttccaagggc aagagacgca atggctccca actggtcact gtgcatggag tttaccgata
    15361 tggggttgaa gtcttttccg tatggagccc atggagatat ttcaagatct acgttctggt
    15421 cccagtaagg tagtattccc atcctctttg aaatgacccc ctgttatttt cttctgaagg
    15481 gattatacat taagaatgtc cgcattgacc cagaaggtcg gacatcttga gatccggtct
    15541 gatcccgaaa cgggtaaggg taaaatcgta tttcgtcggg tcctgaggcg ctatcagccg
    15601 aaaagacctt gtcgcctgat tcgccgtcac catatcaggg gtctttctcg tcacaaggcc
    15661 aagttcctgg cagatcctga tcatatgggt gtcgaggggc aggatgaggg aggccggtga
    15721 aaggcatctc caacctccag gatctatccc gtcatttcgc accatccact tgatcatcag
    15781 attgtacctt ttgcatgcgc taccccttga gacattcgga agaagagaat tcttccgctt
    15841 tcccgagttt ttctgaaggg aacggtacaa cttgcgaagc ccgcccgtaa ctttttcatc
    15901 ctttcccagg cctgaagcaa agagagcctc gatcgaaccc cattcgaaga ggatatcccg
    15961 aatcgacaga agcagttcgt aaatatcatt gcaatcggtg aaacggtgcc tgaaattctg
    16021 gcagatcatt cgaatctcaa ggggggaaga ccctgtcagg aacccatggg gcgaaggacc
    16081 catcggatcc agtatcgccc tggcgctttt cactatcccc gccgcacggc cgtaggcaag
    16141 gcacgaggac acaaccgccg ccacttccct gtcagggaga tcaggataag accggacaac
    16201 acctactggg tccgggtcga taaattcagg ccggttgtac cgatgataca tctcctcgaa
    16261 atgatccgcg aggaactgta tcctctcctc gcaaagatca ggcacctggc agttccatga
    16321 tctttcttcc cacagaaatg cccatttccc tgcactcttc aagatcacca ggggaaggaa
    16381 catactttat cttcaggccc tcatgggcaa ggtctatctt catccggccg agttcctcgt
    16441 taagcagctt tacagattct cctccccagc cgtaggaccc gaaggatgct cccaccctgc
    16501 acatcggcct tagccccctg gcataacata ggagatccgc catgcgcgga aggtaaccgt
    16561 tgttcagcgt cggcgaccca agaaccaggg ccgcggaatc aaggagttct gtcataacat
    16621 cactccgatg ccaccccttc agatcagtag cctttacctc gactccccgg gatatcagtc
    16681 cgtcgatgac tgcgtgggcc attttggctg tgcttcccca catggtgtcg tatgctacaa
    16741 ctgccttggc tttcaccttg cctgatgccc aatccgtgta ggactggagt atatcgggaa
    16801 ctttcttcct ccatataaga ccgtggtcag gggctatcat ttctatcttt attcccatag
    16861 cctttacgtt ctcgagaagt ttaagaacca gaccggagta gggcaggatt atgttggcgt
    16921 agtacttggc tgcctgtttc atcagctctg actgatcggc ttcatcgtca aaacgttcgc
    16981 ccgtcgcata atgctgacca aaggcatcgc tagataccag gagatggtcc tcctccagat
    17041 acgagaacat gctgtcgggc cagtgaagca tctttgtctc gataaacctt acggttctct
    17101 tccccaggga aagggaatcc cctgttttga caacatggaa gggccagtcc tgctttccaa
    17161 aatgttcaat tatggcgtcc atacaggccc cggaacagat tactttttcg ggccgaagtt
    17221 cccgcattac cgaagggaga cttccagtat gatccatctc ggcgtgatta agcaccaggt
    17281 agtccaggtc tccagagggt agaac
//
LOCUS       DAOTIQ010000040        16527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4228_length_16527_cov_8.724138, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000040 DAOTIQ010000000
VERSION     DAOTIQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_16527_cov_8.724138"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..2660)
                     /gene="clpB"
                     /locus_tag="P5541_06265"
     CDS             complement(54..2660)
                     /gene="clpB"
                     /locus_tag="P5541_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRV71623.1"
                     /translation="MDLNKLTIKSQEALSSAQNIAVTMGHTEVDGEHLLLALLRQNGG
                     LIPGLLSRMSISPEKVAEETEKDLSLRSRVSGPGVEPGKINITMRLSKLLVAAEDTAT
                     RMKDEYVSVEHLFMGLIEEGDSTSAGRILRGSGVTSQSFLKVLRETRGNQRVQSADPE
                     NTYEALGKYGRDLVDMARSGKLDPVIGRDEEIRRVIRILSRKTKNNPVLIGEPGVGKT
                     AIAEGLAQRIVRGDVPEGLKDRSVFALDMGSLLAGAKYRGEFEERLKAVLNEIRESEG
                     RIILFIDELHTIVGAGKAEGAIDAGNMLKPMLARGELHCIGATTIDEYRKHIEKDAAL
                     ERRFQPVMVEEPSVEDTVSILRGLKERFEVHHGIRIQDNALVASAVLSDRYITDRFLP
                     DKAIDLVDEASAMIRTEIDSLPADLDAATRRVMQMEIEEAALAKETDKGGIKRLSNLR
                     KELQDARAETDSLRARYDSEKQGIAKLQSIREQIENTRREIERAERDYDLNKAAELRH
                     GKLPELERRLVSEEELLASRQGGEKLLREEVTAEEISRIVSKWTGIPVSRLLEGEREK
                     LLRLDDILHERVIGQEEAVSLVADAVLRARSGIKDPKRPIGSFIFLGPTGVGKTELAR
                     TLAEALFDTEENMIRIDMSEYMEKHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVV
                     LFDEIEKAHQDVFNVLLQILDDGRLTDSHGRTVNFTNTIIIMTSNIGASDLMEGITTE
                     GDIPDGVRERVMRQVRSSFRPEFLNRVDDIVLFKPLRMDEIEQIVDLLAAGLQERLQG
                     MGITLEITPSARRHIAREGYDPVYGARPLKRFISRQVETPVARKIIASSLAEGAKITV
                     DEEGGKIVTCNA"
     gene            complement(2686..3621)
                     /locus_tag="P5541_06270"
     CDS             complement(2686..3621)
                     /locus_tag="P5541_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRV71624.1"
                     /translation="MKFRDYYEILGVSRNATQDEIQKTFRKLARKYHPDVNKASDAEK
                     RFKEINEAYEVLKDPEKRKKYDALGENWKNGQDFSPPPGWDGVFQFGGEGGSGSFSDF
                     FEAIFGRMGGFGPMGGEPFHGRSMRRRGRDLESRIELTVDELVSGGKKHLEFEVLEKD
                     AAGRPLRKKKSLDVTIPRGVTEGSRIRLAGQGEKGAGGASAGDLLLEVHIKKDPCFEV
                     NGQDLRTTVDITPWGAALGSFADVKVPGGSVRMKIPPGTQSGQVMRLKGKGLPGKGDC
                     SGNILVTVRIVVPKKLTPREKELFEALAKESEFRP"
     gene            complement(3699..5597)
                     /gene="dnaK"
                     /locus_tag="P5541_06275"
     CDS             complement(3699..5597)
                     /gene="dnaK"
                     /locus_tag="P5541_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV71625.1"
                     /translation="MSKTVGIDLGTTNSVVAVLEGGHPTVIANAEGSRTTPSVVAFTK
                     DGERLVGALAKRQAVLNPAGTVSSIKRFMGRNHDEVGEEMKIVPYTVVKGSNNSVKIK
                     VEGKEYAPEEISAQVLGKLVRDAAAWLGEKVTDAVITVPAYFNDAQRQATKNAGKIAG
                     LNVLRVVNEPTAAALAYGMDKKNNETVMVFDLGGGTFDVSILDVGEGLCEVRSTAGDT
                     HLGGDDFDKCVVDWLAEEFKKQEGIDLRKDRQSLQRLYEAAEKAKCELSSRTSTDISL
                     PFITADANGPKHLSLSLSRAKFDDLTRHLVERCLEPMKQALRDAKLEAGDINEVILVG
                     GSTRIPAVQEMVRKFTGGKNLNMTVNPDEVVAMGAAVQAGIIKGEVKDVLLLDVTPLS
                     LGIETLGGVMTKIIERNTTIPCRRSEVFSTAEDNQPAVDVLVLQGEREMSKDNRRLGN
                     FKLEGIRPAPRGLPQIEVTFDIDANGILNVSAKDKETGREQKITISNSTNLDESEIDR
                     MIEEAKANAEADKKLREWADARNELEAVSYQVERQISELGDAVPPHEKARGEELVRES
                     KDAVSREEEVSRLRSLTQDLKQVAAALSQHSGTPSGDSESSGTNAGGSTGSGQAEDDV
                     IDAEFNEG"
     gene            complement(5858..6232)
                     /locus_tag="P5541_06280"
     CDS             complement(5858..6232)
                     /locus_tag="P5541_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71626.1"
                     /translation="MNKKSILLALAAVFVIGSVAYAHGGGFGSMGGFGPGMDFDGSGR
                     AAKMGNRNLAMERNSQNIQIPDEIRAKMQELQRTHLEMRLALTQEKPDVNKARLLFGK
                     AQKLQNELSKWRFEEYVKTLSK"
     gene            complement(6338..7846)
                     /locus_tag="P5541_06285"
     CDS             complement(6338..7846)
                     /locus_tag="P5541_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRV71627.1"
                     /translation="MLFSKTLVPTARENPRGISDQSVAKLARAGFVVPDQESRRLELL
                     PMGFMLWKKVISVVSDLARESGFQVVSFDDLGKGSDSISGKIVTSYRHLPLSFIGGDV
                     KKLDAAGFAADKELSKEVRDAFIAIAAQTAGMAGLKVFFGESEGGEVTYASVPCEKGS
                     WHGRGGFVCNSCSWCGDESSPLSLPEAPSGGDAPLEEVETPGADTIAELCRQLGTEPR
                     ETLKTMFYAAGTGDAKEIVAVLARGDHQINDVKLSQVLGGKDVRFADPLEIREAVGDL
                     AGYLGPVGLPGSIRVIADRHVEGCRRLVAGANRPGFHIMNVCWGRDFEARLVADIVSV
                     DESFPCPSCGASISPANIATVASARIGKGASQLSFQDDAGHSCLATRWEGSISMDPLV
                     LAIAGGEKTPSGFAPLDVNVVIASMRNDDAVGLAHLIAEKLEDRGLKVLIDDRDERAG
                     VKFSDAELLGIPVTIVAGRESAERVVEVWQPDGTRGEMPADAAIERAVRWVR"
     gene            8057..8584
                     /locus_tag="P5541_06290"
     CDS             8057..8584
                     /locus_tag="P5541_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71628.1"
                     /translation="MAFSLEPDIIKGSHPEDSFRTGLLQEMLGMLSSLQQEEKIDEFF
                     ILPDFGFDLGIFVSREGTTRPVFFNLKMYMGAKPRAVEIGDQNGSGAEMELLQLNTSR
                     SAMASETFRWILVDITKPKGNRRYTIFTTDQAKEALMGGLNKKKQNSIKLGSVMTFPL
                     TWDELSEKVSSFLAG"
     gene            8656..9108
                     /locus_tag="P5541_06295"
     CDS             8656..9108
                     /locus_tag="P5541_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71629.1"
                     /translation="MSRRASRLEEYFRWAQGKRILVFDPPFWGFHDLFVDKSGSVMLV
                     CLKAEGEAFVFCGDERGAARMQKYGPGPTLNAEEDLEQGIMEWIIYDDFIVYRGPFFP
                     ISRHPYYLGKVAATFPFDEEIRRENQGELISSLFDWYRKHGGEAPKKG"
     gene            complement(9110..10117)
                     /locus_tag="P5541_06300"
     CDS             complement(9110..10117)
                     /locus_tag="P5541_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRV71630.1"
                     /translation="MKEIPVVAVTCSPDREIFPLFDEVIGKRAKVCFIHGAGSEEREK
                     TLERSYAVITTSCAPDELSGSEAGLLKPGSLLQLLFAGADNVPFDVLNENTLVASNTG
                     AFAEPIAEHVLAMTLALVKKIIPRNEALKKGVFDQSGFNGTLRGKVCGILGFGGNGRA
                     VAKAMRSMGMKVAAVNRGGRTEEDVDYIWKNDRLEDLLEISDVLVLALPLTRDTRGII
                     GSRELSMMKKEAILVNIARGAVVDQGALYSHLIDNPDFGAAIDTWWSEPSHHGKFLLD
                     HPFLDLPNFVGSPHVADNVAGMMPAATRFALQNVVDFLEGGTVRGLLDRADYMGMGKP
                     E"
     gene            complement(10154..10969)
                     /locus_tag="P5541_06305"
     CDS             complement(10154..10969)
                     /locus_tag="P5541_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRV71631.1"
                     /translation="MKFCYFDESGMGEEPILVVAGIDVDASRMRTTRKDWADFLDSLS
                     QRTGRPVSEFHTRNFYRGNRLSRTIDGNERAEVISAILDWLSVRRHKITYSAISKARF
                     ESSRSDPRLKDLETPWSAAAFHSVLTLQKAHQGLKGSRRQTVLVFDREVAEEERFSEL
                     IKDPPEWSGEYYGQEHGATPLDTLLEAPLFADSRLVLLIQVADMVAYLLRFYAELQEG
                     LSNESYPGEKQQIEEWAIRIFGMSLPGSFRYPEKCPDGPCGLFWELAPETLRR"
     gene            11119..11625
                     /locus_tag="P5541_06310"
     CDS             11119..11625
                     /locus_tag="P5541_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV71632.1"
                     /translation="MSIRNSDSIKNLLIRRGRINDIPVIAKFIRELAEYEGIADRISF
                     TQESLIDTLFGEKPAAETAMAIVNKEYVGFAVFYYTFSTILGERGLHLDDLYIRPEWR
                     GKGIGTSMMKWLASVAIYRNCGRFEWWCMKTNTTSLDYYSRSGARIIDDIHVLRLEGD
                     EIVKFLKK"
     gene            complement(11647..12114)
                     /locus_tag="P5541_06315"
     CDS             complement(11647..12114)
                     /locus_tag="P5541_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71633.1"
                     /translation="MSWYVRTQKGWGLRWVFLGAFLVLLYRARVRAGSWGNITDSAEF
                     WPLFTSGIILSLILLVFTVMTVTVKESEIEVSMGLELIRKKIPISQILSVTEMMIPWH
                     SVGFKKISGGWLYSVAVSKGLDITLQGGRRVVIGSDDIEGLAEAIRSRMAVPQ"
     gene            complement(12138..12554)
                     /locus_tag="P5541_06320"
     CDS             complement(12138..12554)
                     /locus_tag="P5541_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRV71634.1"
                     /translation="MAGKKKIPVDGSSEPISNTVLGDLLVEKGLVKEKTVKSSGNTGQ
                     KLSPAADGGMPDLSRVGKIVLRVQRKGRGGKTVTLLSCAGLARSGLDPLAREMRKALG
                     CGSRVEEEGIVLQGDVQERAYIWLSERGATRIVGGS"
     gene            complement(12766..12939)
                     /locus_tag="P5541_06325"
     CDS             complement(12766..12939)
                     /locus_tag="P5541_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71635.1"
                     /translation="MKKYLVLSTVLVVLAAVAFFALSQRHENILNLSPEAAEELAGMG
                     GQGILSPEEISSG"
     gene            complement(12983..13345)
                     /locus_tag="P5541_06330"
     CDS             complement(12983..13345)
                     /locus_tag="P5541_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV71636.1"
                     /translation="MLLIVRLLRFLFPLFIGFVMLRLFSRLFYTGAWQGGSRSYSPPP
                     PGSKAPRNPYEVLGCSPRDSDETVRKAYRKLVAKYHPDKFIGLELEKEFVELAARRFQ
                     EIQEAYDHIRESRGFSKN"
     gene            complement(13345..13572)
                     /locus_tag="P5541_06335"
     CDS             complement(13345..13572)
                     /locus_tag="P5541_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71637.1"
                     /translation="MSRAFVNEDSEALVNREAIEQEKKLRDWLRIQEKKLKYLETDPE
                     AEKIDQELREKWIRETREDIERARRQLDGNP"
     gene            complement(13625..15499)
                     /gene="htpG"
                     /locus_tag="P5541_06340"
     CDS             complement(13625..15499)
                     /gene="htpG"
                     /locus_tag="P5541_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRV71638.1"
                     /translation="MPDSKQSFTFQAEAKQLLDLMIHSVYSNPDIFLRELVSNASDAL
                     DKLRFEALTNEALSGLTGDLHIRLDADPEARTLTISDNGTGMTRGDLVEFIGTIARSG
                     TKEYLKILRDKGSSVLPEDLIGQFGIGFYSSFMVAEKVVLLTRKAGEEKAWRWESPGD
                     GTYSLQEDAREEQGTTITLFLKEKSEGGPEKDYSDRQVLKEIIGKYSDFVAWPIRMKD
                     KDGAEETLNSMKAIWTRPESEVSEEEYEEFYRHITRDWNKPARRIFFKAEGGMEFRAL
                     LYIPSRAPLDVFVRDMARGIHLYIRRVFIMDECKELVPEYMRFLRGMVDSEDLPLNIS
                     REMLQQSRQAETIRKSITRKVLDSLSAMLSGDRDAYRTFWSEFGKVLKEGIFSDSRNR
                     EKIMEISLFDTTTAEEPVTLDEYVSRMPEGQDAIYYITAPTSAIGKNSPHLEAFTEKG
                     FEVLVLADPVDEVWTPAVTEYKSRKFVSVARGVVDLGDSGKDVESQGENTDARWDPLL
                     KELEKILGEDVKGVRISGRLKNSPSCLAGEEFDLSPQMEAILRAAGQDVPRVKRNLEI
                     NPSHPLVAKLDRIQEENPADPKVALFARVLYGLASISEGGRVEDPAAFSREVAELLSE
                     "
     gene            complement(15714..16142)
                     /locus_tag="P5541_06345"
     CDS             complement(15714..16142)
                     /locus_tag="P5541_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRV71639.1"
                     /translation="MRGMVPFGGSRNSIFDEMDEVFRGFSDAIRPMTRRFVPVDLYEE
                     GDELFLSVDAPGFDPSQIEIKTFSDRVDIRSKAEGSEEGPEERVWHIRRPARGINLGV
                     SLPTEVDPEQARASFKNGVITVRMPKSRAVQGRVLELREE"
BASE COUNT         3542 a         4735 c         4074 g         4176 t
ORIGIN      
        1 taaatggtga acggtaaaac caaataataa attctgcatc ttccgtttac tgtttacgcg
       61 ttacaagtta cgatctttcc cccctcttcg tcgacggtta tcttcgctcc ttcagcaagg
      121 ctgctggcta tgatcttcct ggcaacgggc gtctcgacct gccgggagat gaatctcttc
      181 agcggccttg ccccgtatac agggtcataa ccctcgcgcg cgatatgcct gcgggcggag
      241 ggggttatct ccagggtaat acccattccc tgcagtcgtt cctgcaggcc tgctgcaaga
      301 agatccacta tctgctctat ctcatccata cgcagaggct tgaacaggac aatatcatcg
      361 acacggttga ggaactccgg ccggaaggac gacctcacct ggcgcattac cctttcgcgg
      421 acaccatcgg ggatgtcccc ctctgtcgtt atgccttcca tgaggtctga agcgcctatg
      481 ttgctggtca ttataattat cgtattggtg aagttcaccg tccttccgtg gctgtccgtg
      541 agcctcccgt catccagtat ctggaggagc acgttgaaga catcctggtg cgccttttct
      601 atctcgtcaa agaggacgac cgaatagggc tttctcctga cggcctcggt caactgtcct
      661 ccctcatcgt agccgacgta acccggggga gcacccacga gcctggagac ggagtgcttc
      721 tccatatatt cggacatgtc tatccttatc atgttctctt ccgtatcgaa cagggcctcc
      781 gcgagggtac gggcaagttc cgtcttcccg actcccgtgg ggccgaggaa tatgaaggat
      841 ccgatcggtc ttttgggatc cttgatcccc gatctggccc ggagcacggc atctgccacc
      901 aggctgacag cctcttcctg cccaatgacc ctttcgtgaa gaatgtcgtc gagacggagc
      961 agtttttcac gttctccttc cagcagcctc gaaacaggta tgccggtcca ttttgatact
     1021 atccgtgata tctcctctgc cgtgacttcc tcacgaagca gtttctctcc tccctgtctg
     1081 gaggccagca gctcttcctc ggagacgagt ctcctttcca gttccggaag ttttccatgc
     1141 cggagttcgg ccgctttatt gagatcgtaa tcccgttcgg ctctttcaat ctcccgtcgg
     1201 gtgttctcga tctgttccct gatgctctgg agttttgcta ttccctgttt ttcggaatca
     1261 tacctggctc tcagcgagtc cgtctccgcc ctggcgtcct ggagttcttt ccggaggttt
     1321 gaaagacgct ttatacctcc cttgtctgtt tcctttgcca gggcagcttc ctctatctcc
     1381 atctgcatga ccctgcgtgt ggcggcatca agatctgccg gcagggagtc tatctcggta
     1441 cgtatcatgg cagaggcctc gtcgacaagg tctatcgcct tgtcggggag aaacctgtcg
     1501 gtaatgtatc gatcggagag gacggcagac gctacaaggg cgttgtcctg gatacgtata
     1561 ccgtgatgga cctcgaaacg ttccttgagt cccctcaata tggaaaccgt atcctcgacg
     1621 gagggctctt ccaccatcac cggctggaat ctgcgttcca gagcggcgtc cttctctata
     1681 tgtttgcggt attcgtctat cgtagtagcc cctatgcagt ggagctcgcc ccgggccagc
     1741 atgggtttca gcatattgcc ggcgtcgata gcgccttcgg ccttgcccgc gccgacgatg
     1801 gtgtgcagtt cgtcgataaa gagaattatc cttccttcgc tctcccttat ctcgttaagt
     1861 acggccttga gccgttcctc gaactcgccc ctgtacttgg cccccgcaag aagagagccc
     1921 atgtccagag cgaatacaga gcggtccttg agcccttcgg ggacatcacc cctgactatc
     1981 ctctgggcga gaccttccgc tatcgccgtt ttcccgacac cgggctctcc aataaggacc
     2041 gggttgttct tggtcttccg gctcaggatc cttattactc gccgtatctc ctcgtctcgt
     2101 ccgataacgg ggtccagttt gccagagcgg gccatgtcaa caaggtcgcg gccgtatttc
     2161 ccaagggcct cataggtgtt ctcaggatcg gcgctctgga ctctctggtt cccccttgtc
     2221 tcccggagaa ccttgagaaa ggactgggat gttacaccgg atccgcgcaa aatacgtccc
     2281 gcgctggtgg agtctccctc ctcgataaga cccatgaaaa gatgctccac cgatacatat
     2341 tcatctttca ttctcgtggc cgtatcttct gcggccacaa gaagtttcga aagcctcatc
     2401 gtaatattta tcttcccggg ctcgactccg gggccggaga ccctgcttcg gagggaaagg
     2461 tctttctctg tctcctcggc gaccttttcc ggtgagatgc tcattcgtga aagaagacct
     2521 ggtataagtc caccgttttg gcgcagaaga gcaagcagca gatgttcgcc atctacctcg
     2581 gtgtgaccca ttgtgacggc gatgttctgc gcgcttgaaa gagcttcctg tgattttatt
     2641 gtcagtttgt tcagatccat tgtgcttgcc tccttgcccc tatgatcagg gcctgaactc
     2701 cgattctttg gccagggcct caaagagctc tttttcgcgg ggggtgagtt tcttcgggac
     2761 aactatcctt accgttacga gaatgtttcc ggaacagtcc cccttgccgg gaaggccctt
     2821 gcctttcaac ctcatgacct ggccgctctg ggtcccgggg gggatcttca ttctgaccga
     2881 gcccccgggg acctttacgt cggcgaatga accgagggca gcaccccagg gagttatgtc
     2941 gacggttgtg cgaaggtcct gtccgtttac ttcaaaacat gggtcttttt ttatgtggac
     3001 ctccaggaga agatcccctg cggaggctcc acccgcaccc ttctccccct ggccggccag
     3061 ccggatcctg gagccctcgg ttacacctcg agggatcgtg acgtcaagag attttttctt
     3121 tctgaggggc cttcctgcgg catctttttc gagaacttcg aattccaggt gttttttccc
     3181 ccccgagaca agttcatcaa cggtgagttc gatcctggat tccaggtccc tgccgcgtct
     3241 cctcatggaa cggccatgga agggttctcc ccccatgggg ccgaaacctc ccatgcgccc
     3301 gaatatggcc tcgaaaaagt cactgaagga tcccgaaccg ccctcccctc caaactgaaa
     3361 tactccgtcc cagccaggag gaggggagaa gtcctgaccg tttttccagt tttctccgag
     3421 agcatcgtat ttctttctct tttccggatc tttcaggact tcgtatgcct catttatctc
     3481 tttaaagcgt ttttctgcat ctgaagcctt gttgacatcg gggtggtatt tccttgccag
     3541 tttcctgaaa gtcttctgta tctcgtcctg ggtcgcgttt cttgatactc ccagaatctc
     3601 gtagtagtcg cgaaatttca ttcaaacacc cctccacccc gatgtcggcc ctcagatcga
     3661 ccgttttgtg ccaacggcac agttatattc tatccgcatc agccctcgtt gaattcagca
     3721 tcaattacgt catcttcagc ctgcccgcta ccggtgcttc ccccggcatt ggtcccggaa
     3781 ctttcactat ccccggaagg agtgccggag tgctgcgaga gggcggccgc gacctgttta
     3841 aggtcctggg tcagggatct cagcctggag acttcttcct ctctggaaac ggcatctttg
     3901 gattccctga ccagttcctc tcccctggct ttttcatggg gaggtacggc atctcccagt
     3961 tcggatatct gcctctccac ctggtaggaa actgcctcga gttcgttcct ggcatcggcc
     4021 cattccctga gttttttatc ggcttcggcg ttggcctttg cctcctctat catgcgatct
     4081 atctcgctct cgtccaggtt ggtcgaattg cttatcgtta ttttctgctc ccggccggtc
     4141 tccttgtctt ttgctgacac gttcaggatc ccgttggcat ctatatcgaa ggtcacctcg
     4201 atctgcggca atcccctggg agcgggccgg ataccctcaa gtttgaagtt cccgagcctt
     4261 ctgttgtctt tggacatctc cctctctccc tgaagaacaa ggacatccac tgcgggctgg
     4321 ttgtcctccg cagtcgagaa gacctcggat cttcggcagg gtatggtggt attcctttct
     4381 attatctttg tcatgacccc gccgagagtc tctatcccga gagaaagtgg cgttacatcg
     4441 aggagcagga catccttgac ctcgcccttg attattcccg cctggaccgc tgctcccatc
     4501 gccacgacct cgtcgggatt gacggtcata ttcagattct tgcctccggt aaatttcctg
     4561 accatctcct ggaccgccgg tatcctcgta gagcctccta ccaggatgac ctcgttgatg
     4621 tctcccgcct cgagtttcgc gtccctgagg gcctgcttca tcggttcaag acatctctcc
     4681 acaagatgcc gggtaaggtc atcgaacttc gcccgggaaa ggctcaggga aagatgcttc
     4741 ggcccgttgg catcagcagt gataaaagga aggctgatat ccgtcgatgt tctgctggag
     4801 agctcacact tggccttttc ggccgcctcg taaagcctct gcagcgactg cctgtccttt
     4861 ctcagatcta tcccttcctg ttttttgaac tcttcagcga gccagtccac gacgcatttg
     4921 tcgaagtcgt caccgccaag gtgggtatct cccgccgtgg agcgcacctc gcatagacct
     4981 tcacccacat ccagtatgga tacgtcgaag gttccccctc cgaggtcgaa aaccattact
     5041 gtttcgttgt tttttttgtc cataccgtag gccagagcgg cggctgtggg ttcattgact
     5101 acccgaagca cattaaggcc cgctatcttg ccggcgttct tggtcgcctg cctctgggca
     5161 tcgttaaagt atgccggaac agttatgacg gcatcggtga ccttttctcc cagccaggcc
     5221 gcggcatccc gaaccaactt tccaagaacc tgggcgctga tctcctcggg ggcatactcc
     5281 ttgccttcta ccttgatctt aacggaattg ttgcttccct tcacgacggt gtagggaact
     5341 atcttcattt cctctccgac ctcgtcgtgg ttcctgccca taaagcgttt gatggaagat
     5401 accgttccgg cagggttgag cacagcctga cgttttgcca gcgcgccgac cagcctttct
     5461 ccatcctttg tgaaagccac caccgatggc gttgtcctgc tgccttcggc attggcaatt
     5521 actgtagggt gccccccctc gaggaccgct acaacggaat tagtggtacc aagatctatt
     5581 ccgacggttt tagacatttc ttttcccccc tgaaagcagg ccgactttat gagtttcaat
     5641 ttggcctgac taatattgac caacacaagg acaattatat aggtcaacat taatcaggca
     5701 attcacctgt acctggtcta tggcgacagg gcggaaatat ttttcgtttt tgggtttttt
     5761 agggtttctt tgactcttta tgggtatttt tggggtgtaa ggtgcacaaa aaagcgggac
     5821 ccctaacgga gtcccgcctg ccgggtcagt tttttctcta cttgctcagg gtcttgacgt
     5881 actcttcaaa acgccacttt gagagttcgt tctggagttt ctgggccttc ccaaagagaa
     5941 gcctggcctt gttgacgtca ggtttttcct gggtaagagc cagcctcatt tcgaggtgag
     6001 ttctctgcag ttcctgcatt ttggcacgga tctcatcggg tatctggata ttctgggagt
     6061 ttctttccat ggccagatta cggttgccca ttttagccgc ccgtcccgag ccatcaaaat
     6121 ccatccccgg accaaaacca cccatgctgc cgaaaccgcc accgtgggca taagctacgg
     6181 aaccgatcac gaaaaccgcc gccagagcca gaagaatact ttttttgttc atccctttca
     6241 cctccatcag tctcgctcaa tatccctgat ggggtcattc taggacctcc tgtttccctg
     6301 gtaatgggta gaaaagccga ttcccgaatg gggaaaacta ccgaacccag cgaaccgctc
     6361 gctctatcgc cgcatcggcg ggcatttctc ccctggtccc gtcgggctgc cagacttcta
     6421 caactctctc agcggattcc ctcccggcaa cgatcgtaac cggtatgccc agaagttcgg
     6481 catcggaaaa cttgactccg gctctttcgt ccctgtcgtc gatcaggacc ttgaggccac
     6541 gatcttccag tttctccgca atcaggtgcg ccaggccaac ggcatcgtcg ttcctcattg
     6601 aggcaataac tacgttcaca tccagcgggg cgaatccgga gggtgttttt tctccacccg
     6661 cgatcgcaag caccagaggg tccatgctga tcgatccttc ccaccgggtg gccaggcagg
     6721 agtgcccggc gtcatcctgg aaagaaagct gtgaggctcc ctttccgatc ctggccgagg
     6781 caacagtggc aatgttcgcc ggtgaaatgg aagcgccgca ggatggacag gggaatgact
     6841 catctacact tacgatgtcg gcgaccagcc gtgcctcgaa atcacgaccc cagcagacgt
     6901 tcatgatatg aaatcccggc ctgttggctc cggcgacaag cctccggcag ccttcgacat
     6961 gcctgtcggc aattaccctt atgctccccg gcaaacccac cggacccagg taccccgcca
     7021 ggtcgcctac ggcctccctt atctccagcg gatctgcaaa gcggacatct tttccaccaa
     7081 gcacttgaga aagtttgacg tcgttgatct ggtggtcccc cctggccaga acggcaacaa
     7141 tttctttggc atcccccgtt ccggcggcgt agaacatggt cttgagggtc tcacggggtt
     7201 ctgttcccaa ctgacggcat agttccgcga tggtgtcggc accgggagtt tcaacttcct
     7261 caagaggcgc gtctccccca gacggggctt cgggcagaga aagcggagag gactcgtcgc
     7321 cacaccagga acaggaatta cagacaaaac cgcctcttcc atgccaggat cccttttcgc
     7381 aagggacaga ggcgtatgtc acctctcccc cttcagattc tccgaaaaag actttcagcc
     7441 cagccattcc tgcagtttga gctgcaattg caatgaaagc atctctaacc tctttcgata
     7501 actctttgtc cgcggcaaat ccggcggcat ccagtttttt gacatctccg ccaatgaaag
     7561 aaagaggcag atggcgatag ctcgtcacaa tctttcccga aattgaatcg gacccctttc
     7621 caagatcatc aaaggagact acctggaaac cgctctccct cgcgaggtcc gatacaacgg
     7681 atattacttt cttccagagc ataaatccca tcggaagcag ttccagtctc ctgctctcct
     7741 ggtcaggaac cacgaaaccc gctctggcca gttttgccac ggactgatcc gagatccctc
     7801 tggggttttc cctggccgtt gggacaagag tttttgaaaa tagcattttt actacctccc
     7861 ggttggtgtg caatatcgtc ttccccctgt ggggctccct aaagtatagg ccatgatggg
     7921 gcgtaaggtc attcccacct gtattggggt ataatcgaat ccgaagtttt ccgatctgtc
     7981 gaatcagaga acttcccccg aacaacatga tccgggggat tcatattctg aaaaacccga
     8041 aaggacggtg gttttcttgg ctttcagcct tgaaccggac atcattaaag gctctcaccc
     8101 ggaagacagt ttcaggacgg gactcctcca ggagatgctt ggaatgctct cctccctcca
     8161 gcaggaggaa aagatagacg agtttttcat tctcccggat ttcgggttcg accttgggat
     8221 ctttgtcagc agggaaggaa caacacgccc ggtctttttc aaccttaaaa tgtacatggg
     8281 cgcgaaaccc agggccgtcg aaataggaga ccagaacgga tcgggagccg aaatggaact
     8341 acttcaactg aacacatcaa gatccgccat ggcttccgag acattcaggt ggatactcgt
     8401 ggatataacc aaacccaaag ggaacaggcg ttacaccatt ttcaccacgg accaggcaaa
     8461 ggaagctctt atgggcggcc tcaacaaaaa gaaacagaac agtataaagc tgggttcggt
     8521 gatgaccttc cctcttacct gggacgagtt gtcggaaaag gtctcttcgt ttctggcagg
     8581 atagcccgct aaattccgat gtccttattg ggttctcttt tcagaaagaa aaaacttccc
     8641 ctctccccgg atgagatgtc gcgacgcgct tcccgcctgg aggagtattt ccgttgggcc
     8701 caggggaaaa gaatcctggt ctttgatccc cctttctggg gtttccacga tcttttcgtg
     8761 gacaaaagcg gcagcgtgat gctggtctgc ctgaaagccg agggcgaggc tttcgtcttt
     8821 tgcggcgacg aaaggggcgc ggcaaggatg cagaagtacg ggccgggccc aaccctgaat
     8881 gccgaggaag acctcgagca ggggatcatg gaatggatca tctacgatga cttcatcgtt
     8941 taccgtggcc cctttttccc catcagccga cacccatact accttggaaa ggtcgcggcg
     9001 acattcccct tcgatgaaga gatccgtcgg gaaaatcagg gagaacttat ctcgtcactt
     9061 ttcgactggt acagaaaaca cggcggggaa gccccgaaaa aagggtaaat cactcaggtt
     9121 tgcccatacc catgtagtcg gccctgtcaa gaaggcccct tacagttcca ccctccagaa
     9181 agtcaaccac gttctgaagc gcgaaacgtg tcgccgcggg catcattccg gcaacattat
     9241 cggctacgtg gggagatccc acgaagttgg gcaggtcaag gaaggggtga tccagaagga
     9301 acttcccatg gtgagagggt tccgaccacc atgtatctat cgccgcccca aagtccggat
     9361 tgtcgatgag gtggctgtat agggcgccct gatccacgac agctcccctg gctatattga
     9421 ccaggatcgc ttcttttttc atcatggaga gttcacgact tccgataata cccctcgtat
     9481 ctctcgtaag gggaagggcc agaacaagga catcggatat ttcaaggaga tcttccagcc
     9541 tgtcgttctt ccagatataa tcgacgtcct cttcggtcct tccgcccctg ttgaccgccg
     9601 ccaccttcat ccccatggat ctcatggcct ttgcgaccgc cctgccgtta cccccgaaac
     9661 ccagaatacc gcagaccttc cccctgaggg tcccattgaa accgctctgg tcaaaaacac
     9721 cttttttcag cgcctcgttc ctcgggatga tctttttcac cagcgcaagg gtcatggcca
     9781 gaacgtgttc ggctattggt tcggcaaaag ccccggtgtt ggaggcgacc agagtgtttt
     9841 cattcaatac gtcaaagggg acattgtccg ctccagcgaa aagaagctgc agcagagagc
     9901 cgggcttcag gaggcctgcc tcgctaccgg aaagttcgtc gggggcacaa cttgtggtaa
     9961 tgaccgcata ggacctttca agggtttttt ccctttcttc cgaacccgca ccgtgaatga
    10021 aacagacttt ggcacgttta cctatcactt catcaaaaag ggggaagatc tcccgatcag
    10081 gggaacatgt cacggccaca acaggtattt ctttcaagag agaacctcct tcgagttttt
    10141 ccgatttgcg gtttcaacgc ctcagggtct cgggcgcaag ttcccagaac aggccacatg
    10201 ggccatcggg gcatttttca gggtacctga aacttccggg aagagacatc ccgaatattc
    10261 ttattgccca ttcctctatc tgctgttttt ctcccgggta actttcattc gagagtccct
    10321 cctgaagctc tgcataaaac cgcagaaggt aggcgaccat gtcggccacc tgtatgagca
    10381 gaaccagtcg cgagtcagcg aaaagcggcg cttccaggag ggtgtcaagg ggggtcgccc
    10441 catgctcctg accatagtat tctccggacc attcaggcgg atccttaata agttcgctga
    10501 agcgctcctc ctcggccacc tccctgtcga agacaagaac ggtctgtctt cgtgaaccct
    10561 tcaggccctg gtgagccttc tgcagcgtga ggacactgtg gaaggcggcc gcactccatg
    10621 gagtctcgag atcttttagc ctggggtcgg atctggaact ttcgaagcgt gctttactga
    10681 tggcactgta ggttatcttg tgacgccgaa ccgacagcca gtcaaggata gcagagatca
    10741 cctccgccct ttcgtttccg tcgatggtcc gggaaagcct gttccccctg tagaaattcc
    10801 ttgtatggaa ctcgcttaca ggccttcccg tcctctgtga gagggaatcc agaaagtcgg
    10861 cccagtcctt cctggttgtc ctcatgcgac tggcatcaac atctatgcca gctacaacaa
    10921 ggattggctc ctcccccatt ccgctttcat cgaagtaaca gaatttcatc tttgcgaccc
    10981 ccaaggtcag taaaggtcta aggtttcttc tcttttattt tttgtttttt tgcaaccctt
    11041 tgaaaaattg cccttttgaa ttataaatga cttacttcca gagtatcatt ctaccaattc
    11101 cgacggagga ttgagatcat gtctatcaga aacagcgact ccatcaagaa tctgctcata
    11161 aggagaggcc ggatcaacga tattcctgtt attgcaaagt tcatacgaga actggcagag
    11221 tacgagggaa tagcggaccg catttcattt acacaggaaa gccttatcga tactcttttc
    11281 ggagaaaagc cggccgcgga gacggcaatg gccatcgtaa acaaggagta tgtgggattt
    11341 gccgttttct actatacttt ctcgacaatt ctcggcgaac ggggtctgca tcttgatgac
    11401 ctttatataa gaccggaatg gcgcggcaag ggtatcggga cgtcgatgat gaaatggctc
    11461 gcgtcggtcg ctatctatcg gaattgcgga agattcgaat ggtggtgcat gaaaacaaac
    11521 accacctccc tggattacta ctccaggagt ggggccagga tcatcgatga tatccatgta
    11581 ctgaggctgg aaggcgatga aatagtgaaa tttctgaaga agtgaatctt cgctcgcgta
    11641 ccggggtcat tgaggcacgg ccattctcga cctgatggcc tctgcgaggc cttcgatatc
    11701 atcggaacct atgacgaccc gtctcccacc ctgaagggtt atatccagcc ctttcgatac
    11761 ggcaacgctg taaagccatc ccccggatat cttcttgaaa ccgaccgaat gccagggaat
    11821 catcatctcg gttactgaaa gtatttgcga tatggggatt tttttacgga tcagttcaag
    11881 ccccatggag acctctatct cgctctcctt gacggtaacg gtcattaccg tgaagaccag
    11941 aaggatcagc gaaaggatga tccccgaggt aaaaagaggc cagaattcgg ctgaatctgt
    12001 tatgtttccc cacgatcccg ccctgaccct tgccctgtaa agaaggacca ggaacgcgcc
    12061 aagaaatacc catctcaagc cccagccctt ctgggtcctt acataccatg acattgatct
    12121 gttcctcccg gaattattca ggagccgccg acaattctgg tcgctcccct ttcggagagc
    12181 cagatgtagg cccgttcctg gacatcgccc tgaagcacta tcccttcttc ttcgacacgg
    12241 gatccgcaac ccagagcctt tctcatctcc ctggccagag gatccaggcc agaccttgca
    12301 agaccggcac aggaaagaag ggtcaccgtc ttgcctcccc ttcctttgcg ctgaaccctc
    12361 aacactatct tacccaccct ggaaagatcg ggcatccctc cgtccgctgc cggagaaagt
    12421 ttttgccctg tgttccccga tgattttacg gttttctcct ttaccaggcc tttttcaacg
    12481 aggaggtctc caagaactgt attggagatg ggctccgagg acccgtcgac agggatcttc
    12541 tttttgcctg ccattttctc agacctcttt tcagggacct ggaacaaaat tggtcgcata
    12601 taccaccagg ccttcactgg catacccgat atccaggtct atacccctgc cccgaaactt
    12661 tagcgacctg tcgacccaaa gaagtacccc gctaccctcg acaagtctgt atctacctgt
    12721 atcctctgga gtgccgcccc gaaccagggc gacgaccgtg cggcctcagc cactggaaat
    12781 ttcctcgggt gaaaggattc cctggccacc catccccgca agctcttcag ccgcctcggg
    12841 cgacagattc agaatatttt catgtctctg tgaaagggca aaaaaagcca cggcagccaa
    12901 tactaccagg accgtcgaca ataccagata ctttttcatg gactctctcc tcctctcggc
    12961 tatctgttgt catggtcttt tactaatttt ttgaaaaccc tctggactcc cgaatatgat
    13021 cgtaggcctc ctgtatttcc tggaacctcc ttgcggccag ttctacaaat tctttttcaa
    13081 gttcaaggcc tatgaattta tcgggatggt acttcgcgac aagtttacgg taggctttcc
    13141 ggacagtctc atcggaatcc cggggagaac accccagaac ctcgtagggg tttcggggtg
    13201 ccttgctccc tggcggagga ggagagtaac tccggcttcc gccctgccag gcccccgtgt
    13261 agaagagtct ggaaaatagc ctgagcatga caaacccgat aaacagcggg aacaggaacc
    13321 tcaaaagccg tactatcaat agcatcaggg gttaccgtcc agttgccggc gagcccgttc
    13381 gatatcctcg cgggtctccc tgatccattt ttccctcagc tcctggtcta tcttttcggc
    13441 ctcaggatcc gtttcaagat atttcagttt cttttcctgt atgcggagcc agtcccgaag
    13501 ttttttttcc tgttcaatgg cctcgcggtt cacaagggct tcgctgtcct cgttgacaaa
    13561 ggccctggac atatagccca cccccctgaa gattataaac tctcagcccc aagttgccct
    13621 ccgcttattc ggatagcagt tccgcgacct ctcggctgaa ggcggccgga tcctcgaccc
    13681 ttccaccttc ggagatagag gcaaggccgt acaggacacg agcgaacagt gccaccttcg
    13741 gatctgcggg gttttcttct tggatccggt cgagtttggc aacaagcggg tgggacgggt
    13801 tgatctcaag gtttctttta actcttggaa catcctgccc ggcagccctg agaatggcct
    13861 ccatctgcgg cgagaggtcg aactcctctc cggcgagaca ggagggggaa tttttcagtc
    13921 ttcctgatat tcgcaccccc ttgacatcct ctccaaggat cttctccagc tcctttagca
    13981 agggatccca cctggcatcg gtgttttccc cctggctttc cacgtctttt ccagaatcgc
    14041 ccagatccac tacgcccctt gcgacggata caaattttct gctcttgtat tctgtgacgg
    14101 caggcgtcca gacttcgtcg actgggtcgg ccagaacaag tacttcgaac cctttttccg
    14161 taaaagcctc caggtgtggg ctgtttttgc caattgccga agttggtgcc gtgatgtaat
    14221 agatagcatc ctgaccctca ggcatacggg agacatattc atcaagagtg acaggttctt
    14281 cggcggtggt cgtgtcaaaa agggatattt ccattatttt ctcccggttg cggctgtccg
    14341 agaaaatacc ctccttaagg acctttccga actcactcca gaaggtacgg taggcatccc
    14401 tgtcgccgga gagcatggcg gaaaggctat ccagaacctt tcttgtaata cttttacgta
    14461 tagtctcggc ctggcggctc tgctgaagca tttcccggga aatattcaga ggcaggtcct
    14521 cggaatccac catccctctg agaaacctca tgtattctgg gacaagttcc ttgcactcgt
    14581 ccatgatgaa gacccggcgg atgtaaagat gtatcccccg tgccatatcc ctgacgaaaa
    14641 catcaagggg agcccgcgag ggtatataaa gaagagccct gaactccatg cccccctcag
    14701 ccttgaagaa gatcctcctg gcaggcttgt tccagtccct cgtgatgtgt ctgtagaatt
    14761 cctcgtattc ttcctccgaa acctcgctct cgggcctcgt ccagatggcc ttcatcgagt
    14821 tcagtgtctc ttccgcgccg tccttgtcct tcatgcggat cggccaggcc acaaaatcgg
    14881 agtattttcc aatgatctcc ttcagaacct gcctgtcgga ataatccttt tcgggcccac
    14941 cttcactctt ctccttcagg aaaagagtta tcgtggttcc ctgctcctcg cgggcatcct
    15001 cctggaggga atatgttccg tcaccggggg attcccatct ccaggccttc tcttctccgg
    15061 ccttgcgtgt aaggaggacc actttttctg caaccatgaa agaagaataa aaacctatac
    15121 caaactggcc gataaggtcc tcgggcaaaa cggaagatcc cttgtcacgg aggattttca
    15181 ggtattcttt ggtacctgaa cgggcgatgg tgccaatgaa ctccacaagg tcgccgcggg
    15241 tcatgcccgt accgttgtcg gagatggtca gcgtacgggc ttcagggtcc gcatccagcc
    15301 ttatgtggag gtcaccggtg agtcccgaga gagcctcatt tgtgagggcc tcaaatctca
    15361 gcttgtccag tgcatccgag gcattggata ccagttccct gaggaaaata tccggattcg
    15421 agtaaacaga gtggatcata agatcgagga gttgtttcgc ctcggcctga aatgtgaaag
    15481 actgtttgct atcgggcatt tttagaacct ccttgacaaa aacttaaata atcaggattc
    15541 aaaaggcgta aacggtcaac ggaaaacgga aaaagggcaa gaatcaagtg cgtacaccct
    15601 gaactgagaa tcattgatcc gccactcggc ggaattcaaa caaaaagggc ggcgggataa
    15661 ccgccgcccc gagattgtag cctatcggag gaattttcgc atacccttgg gtattactct
    15721 tccctgagtt ccagaaccct gccctgtacg gccctgctct ttggcatccg taccgttatc
    15781 acgccgtttt tgaaggatgc cctcgcctgc tcgggatcga cctctgtcgg gagagatacg
    15841 ccaaggttta tgcctctggc gggcctgcgg atatgccata ccctttcctc ggggccctct
    15901 tccgatcctt cagccttgga cctaatgtct accctgtcgg agaaggtctt tatctcaatc
    15961 tgggagggat caaaaccggg agcgtcaacg ctaagaaaca gctcgtctcc ctcctcataa
    16021 agatcaacgg gcacgaaacg acgggtcata ggcctgatag catcggaaaa accgcggaaa
    16081 acttcatcca tttcgtcaaa aatcgagttt cttgatcctc caaacggaac catacccctc
    16141 atttatatcc cctccttgaa atctcctgat ggcgagaata tatcaattta tgtcagggta
    16201 ggtcaaatta ctaggtcagt aatggttaag agttttggct ttgtatggct aaatattctg
    16261 gttttttggc tggttatttt gattttttat tgttttctcg ggatatcggg tgaggaaaca
    16321 ggagacggaa gactggaggc aggaagggca aaaaagccgt tagacgtaag acgtgagaag
    16381 tgagacgacc aaattcaact gcgtaaacgg tcaacggtaa acggtaaacg gttaaagagc
    16441 gagacgtcag gaaactggag gcagggaagg tctttggttc tcacaaagaa aagaagggtt
    16501 ttcaggcgag ttgcctttcg gaaaaga
//
LOCUS       DAOTIQ010000041        16358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4284_length_16358_cov_7.410538, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000041 DAOTIQ010000000
VERSION     DAOTIQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_16358_cov_7.410538"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..962
                     /locus_tag="P5541_06350"
     CDS             405..962
                     /locus_tag="P5541_06350"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRV71640.1"
                     /translation="MDQLFINPEDADFAKKDFIFRVSTATVDVEESLFTFFDNYDRII
                     MTTDKEFVPLHNDKDEVFLNKYELQLFYGGDKTQGKGKVNDYNFIMKRGVCRGDTMAM
                     SLAKGSLVYPRKGNDKCDETLEIVYCARGRLTFRYNDIEEYLVLGDVLVIDHGTMANK
                     YVLSNEDDQFCDLVITMARIDHLEK"
     gene            complement(1073..1471)
                     /locus_tag="P5541_06355"
     CDS             complement(1073..1471)
                     /locus_tag="P5541_06355"
                     /inference="COORDINATES: protein motif:HMM:NF021602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HRV71641.1"
                     /translation="MTKPGKQQGSGLAIRHEEYSGILPRPGDFSGYESVLPGSAERIL
                     KMAEEQSMHRREMEKIVIRSRSRDSLCGIICGFLIGVTTVLSGAFCIIKGHPYPGTFL
                     GTAGLVGLVGVFIYGTSSNRKEREQKFSQR"
     gene            complement(1455..1658)
                     /locus_tag="P5541_06360"
     CDS             complement(1455..1658)
                     /locus_tag="P5541_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71642.1"
                     /translation="MSFTDQLFAMPGFFGGMASALDMGNTLTIFNESCTPEEADAMAI
                     RSDWKIVGQDLRSAMQVVDDETR"
     gene            2009..3745
                     /locus_tag="P5541_06365"
     CDS             2009..3745
                     /locus_tag="P5541_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRV71643.1"
                     /translation="MSDMELAKLTEPGIRKVAGERIFSRGEAYYAGGAVHETVREGSA
                     IRALCEGSRSSPYRVRAVLSESGIESASCTCPMDGFCKHIVAMLLKFIDEPDDFREMP
                     PLEDILSSLSREEMASVISSMVECEPRLVTLVTRAAAKTGAANDLDGLRHDIREAMFG
                     DDPFQILMRLTGIMSMAHKLAEKGDWHGAGSVYHELLRELAPVYEEYLLPLDDDGDIA
                     ALACDCVEGLEVSLREKGISKEARYEWFESLLDAMLADIHIGGVSFAEGSLDVLMDFA
                     GEEDWLSLEPTVLDLIPASEGWTREALVNVLAAWRKKWVRSDEAREVIREKGSSRQKV
                     FSLVQDGRFEEAVSLARAHLEDQPGVMLDLADALVVAKAPECAVELLLQYASSEKQGC
                     YRFLCYRQWLAEYYRKSRNHKKALEWQRKLFFDDPSLEMFSVLQKLGKTMKVWQEVRA
                     EALDYLETIKRFGVLIDIALYEGDIQTALDLLPRLPRASCPDPRLDVAQAAEKTLPLE
                     AIRLYKDLAEKAIALRHRESYGMAARYLVCVKKLYEKLDRIGEWREDLASLKNRYTRL
                     PALQNELKKAGL"
     gene            3762..4988
                     /locus_tag="P5541_06370"
     CDS             3762..4988
                     /locus_tag="P5541_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71644.1"
                     /translation="MSSNIKHKWVFRPRFRRGAFGWRSQPAITRIKEAVSEIKKVASK
                     NPILGGEGAVLFLEKVSGALENIDSSSGAIGSAVNWAIESLVPIVANAPADGALREGW
                     LERLWSAVQADDMSYIESLADYWGELCATPECASRWADSFIETVRMVWGPGKELSGYY
                     HGIPACLSSLFKAGRNDEIMDLLKQAPYKTWHQRIWGVKALFATGKRSEAIRFAEGSR
                     ESNQPDFRISEACEEILLASGMVEEAYERYALTANQKTTYLATFRAIAKKYSHKEPAV
                     ILKDLVARSPGNKGKWFAAAKSAGLYEEAISLANSSPCDPGTLARAARDMAETRPLFA
                     KDAGIAALRWLAAGYGYEITSYDVVAAFDHTVKAAKKAGCLSETIEMIREMVSGEKPC
                     DRLVSTILERELKLID"
     gene            complement(5081..5824)
                     /locus_tag="P5541_06375"
     CDS             complement(5081..5824)
                     /locus_tag="P5541_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRV71645.1"
                     /translation="MTGKGKVREVNEDSLIVGSRLIGCGEMLEPKICQIADVPFLAVA
                     DGVGGGPGGKEASFGALEAILENISLLNCEDPEPVLDKAVEKLIEISALKPELSGLAT
                     TLAALYINLPLNCFMAFGCGDSRVYRQNGPFLQQITKDHSLVREMADSGLITGEEMLT
                     HPMRNVITSSISGRNEKKTRYSSFSQSLKEKERFFLCTDGVWEYLDIKNLERCLQTKD
                     IFSAAKEIADLIMSSPATDNFTFIVTDIG"
     gene            complement(6495..7070)
                     /locus_tag="P5541_06380"
     CDS             complement(6495..7070)
                     /locus_tag="P5541_06380"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRV71646.1"
                     /translation="MQKEPEKKECCSCGSGKDNSQKNNKDIISLGSFDVAGKGPGSYL
                     GGVIKLRADLKKDLLDSAENFLKASDRCLNGCKIEEGVEILTVPGTVCSALSCELFLK
                     YIILIERDREFKDHDLVFLFGQCRRETQSALSELIADILNILERNKDHFVDARYHHER
                     DYFPFRPQELLQTAEVFSKFVKSKYPHGEST"
     gene            complement(7249..7605)
                     /locus_tag="P5541_06385"
     CDS             complement(7249..7605)
                     /locus_tag="P5541_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71647.1"
                     /translation="MKKMIAAVCFISLVLGIAMPAFAFFDNGEIAPGIFFDDVEVNNN
                     GQVKGVVVNKTDQYICLSASILFCDIFKKAVGDAYVSCLDVLPKGKAVFQTVLLNGSG
                     KDAKHAHKIVWIVKRL"
     gene            complement(7625..7963)
                     /locus_tag="P5541_06390"
     CDS             complement(7625..7963)
                     /locus_tag="P5541_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRV71648.1"
                     /translation="MKPIGTESFDEDKVKQGSYALKTVGHSLEGDGIMEGDIVLVNPY
                     QGDDIKPEWIMYVIFDGSPMLKHVIYHRSGAVELRGSNPKESPRLIPPEMVSGYFTIC
                     GRVVRLKRNF"
     gene            complement(8713..10218)
                     /locus_tag="P5541_06395"
     CDS             complement(8713..10218)
                     /locus_tag="P5541_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRV71649.1"
                     /translation="MTFSPSMTSGVTGAKNRSKYISPQSGMCSLCTEECPGTCEITQA
                     AVLGKMTVYPTTTGANQIASEKDYPVDFSHFNINGRCFGSMGTEADHEHAEIFNVDLA
                     SEYGTDNIIKLDLPVVLPALVKMNWVDYFGGAAMAGVTCMVGEDARNKDPELKIEKGK
                     ITSFPMLKKIHDSFYNYHRGPGQMVLQCNVDDHLVGVPEIALNKYGFEALEFKFGQGA
                     KGTQPMNRIKDYDEAVKKVERGDLVFPDPFDPKIIELKKKGYCPNFYTYGRLPMWTEE
                     SMAERISELRAIGMKNVYFKMAGFDVIDVERVINMAIENRVDMITFDGAGGGSGYSPA
                     KMMNEWCLPTVVLEKVVVDICAKLQDQDKTLPAMVMTGGFISEDQIFKSLAFGDGFIS
                     AVGVCRGAMAAAMTGKTVGDEIKAGRTPELFKKFGSTIDEVFHDLADLRGIYGKEADD
                     FSTGAIGVYSYLNKLGCGLRHFAALNRKFRLEYLDRSDLIPLTYYANDLLS"
     gene            complement(10432..11325)
                     /locus_tag="P5541_06400"
     CDS             complement(10432..11325)
                     /locus_tag="P5541_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71650.1"
                     /translation="MKIIRYLATGILIALAAAGTAFGDAAPDPLNSGLAPSARTHRVW
                     MADEEVNIHLGEETVSVEAIFRFANLSEKKTTLEVGFPTDYRDDVEGLEVFVNGNVVA
                     FREEKEKLFYPESIDEDGITVNWALWDMTFPGKTETELRVTYRVKPRKNHDYLITPYR
                     DFIEKIVTGMPQEPSADSAVGRLLAGMETFSTGYILVTGSGWYGSIGEAVINVFHEAK
                     GPGVIRWFHPEEDYRFLPDRLQWKFYYLDPTFNIELEFNPSFTLDEEISLVSEALASD
                     GKNQGLKDLMNFLQELRGKMK"
     gene            11508..12812
                     /locus_tag="P5541_06405"
     CDS             11508..12812
                     /locus_tag="P5541_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRV71651.1"
                     /translation="MKFALCDCNNFYASCERVFRPDLRDRPVVVLSNNDGCIIARSAE
                     VKAMGIKMGAPYFKERYRLESAGAAVFSSNYALYGDMSRRVMETLGTMVPDVEIYSVD
                     EAFLLLDSAPLRGSTAEELCRHVKKQVFRWTGIPVSLGLGATKTLAKAANRIAKKESS
                     CGGVFAIPEGVGADVFLAGLNVEDVWGVGFRYAPMLKRSGIRTALDLKMADDEWIRRR
                     MTVCGLRTVWELRGTVCHPFRACPDPAKSIRSSRSFGEPVISLHHLREAVASFAERAC
                     ETLRSQCSTAGCISVFIATSPHREDPQYANAATRRLAMDSDFTPAIITAAMECLEEIY
                     VPGLQYVKAGVLLRELRSALTRQSGLFDVAETGRRVRLMAAIDRINGDLGRGRISVAA
                     SGRWKPRVWDMKQDLVSFCYTTRWEDLAGVRVVGGEVPPCRN"
     gene            12800..13150
                     /locus_tag="P5541_06410"
     CDS             12800..13150
                     /locus_tag="P5541_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71652.1"
                     /translation="MQELKITGFPSPAASWSEKPLDILNLLAPNPVSTFFMRLEEDVP
                     QWALLEGDILVVDRSLEPRTGSLAVVVENGALVLRVIGRSGARRSFALPEGKESSGID
                     IETWGIVRGVARLW"
     gene            complement(13164..13715)
                     /locus_tag="P5541_06415"
     CDS             complement(13164..13715)
                     /locus_tag="P5541_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRV71653.1"
                     /translation="MKNRSESSRNFAQWFSFVTIRLMHDNRLLEKFVHPAEALRNLGL
                     REGDRVFEPGCGPGFYTVGAAKAVGKSGEVVSFDVNPYACKFAREKVQKLSLGQVRVE
                     NHNAADSGLGDRTIDFAFVVGVPHVVGGEHNLMDELGRVVKPGGTMAYRPGRGNKARL
                     VEAAVKRGFRLETTSRRFLVFRK"
     gene            complement(13712..14197)
                     /locus_tag="P5541_06420"
     CDS             complement(13712..14197)
                     /locus_tag="P5541_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV71654.1"
                     /translation="MIGEKKALEMIEKILWMTEEYVQLAAMPKHYGTGDLFHNLDIHI
                     IHTIGKNRGINVTGLARKHSISKSAVSQAIAKLQRRGLVRRYQVPENRKEVLFELTDR
                     GERAFEAHLDFHMRAETPYINELARFSREESAMVEKVIDLLMRRARHVRTLISNKEAE
                     E"
     gene            14591..16234
                     /gene="hcp"
                     /locus_tag="P5541_06425"
                     /gene_synonym="priS"
     CDS             14591..16234
                     /gene="hcp"
                     /locus_tag="P5541_06425"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRV71655.1"
                     /translation="MYCFQCQETAKNQGCTVRGVCGKPEGTADLQDLLIYVCKGISVF
                     GEELRKSGTVDRDAGRFICRALFVTITNAAWDDDSIIEWIREGLRVKEAIAGRVGDLP
                     GKLPDCATWKADSKEEIMTKSMSDEVRVTATENDDVRSLRELVIIGSKGVAAYAEHAE
                     VLGFEKDEIYEAIMEGLASTTKDLSLDEMVAMVMKTGENAVTTMALLDEANTSTYGSP
                     EISEVNLGVRNNPGILISGHDLKDMDELLKQTEGTGVDIYTHGEMLPANYYPAFKKFE
                     HFVGNYGGSWWHQNEEFESFNGPVLLTTNCLIPLKKENTYLDRLFTTGVVSYPGAKHI
                     PDRPKGGKKDFSGLVKMAKKCAPPEELEKGKIVGGFAHNQVMALADKVVEAVKSGAIS
                     RFVVMAGCDGRFRSRDYYTEVARNLPRDTVILTAGCAKYRYNKLDLGDIGGIPRILDA
                     GQCNDSYSLAVIAMKLKEAFGFADINELPISYDIAWYEQKAVAVLLALLSLGIKGIRL
                     GPTLPAFLSPNVVKVLVDKFDIKPIGTVEEDITAMMAGK"
BASE COUNT         3930 a         3924 c         4309 g         4195 t
ORIGIN      
        1 gcttagaccg gcaagtatcc tgtagattgc ctttctctct ttacgaaggt cttcgttgac
       61 tgctggcatt gtctgatgcc tccctgcctg tatcatgtgg cataaagtcg cctgtttgtt
      121 tattgtatca gccagttttg tattgttcca gcaaaccctg cgtttttcgg agcaactctg
      181 tcaccatagg tctcaggagc ctgcactttt gttggttacg ctgcttctgt tggtaagatc
      241 gagtagaaaa gcagttataa gcgcgtagtg gcagtcctta ccccatcaag ctttctatcg
      301 agcaggaggc aaaaagagat gaaagaaaga cctttcctga aggatacccc ctattggatc
      361 atcagaaagg ataaggtgaa aaaaggtcgt ggtccggaga caccgtggat cagctgttca
      421 ttaaccctga ggatgctgat tttgccaaaa aggattttat ctttcgggtc tccacggcca
      481 cggtggatgt cgaggaatcg cttttcacct tcttcgataa ctacgaccgg atcatcatga
      541 ctaccgacaa ggaatttgtc ccgctccaca atgacaagga tgaggttttt ttgaacaaat
      601 acgagctcca acttttttat gggggagaca aaacccaggg caaaggaaag gtcaatgact
      661 acaacttcat tatgaaaaga ggggtctgcc gcggggatac tatggccatg tccctggcaa
      721 aggggagttt ggtctatccc cggaagggga acgacaaatg cgatgagacc cttgaaatag
      781 tctattgcgc cagggggagg cttaccttta gatacaacga tatcgaggag tatcttgtcc
      841 tgggggatgt cttggtgatc gaccacggga ctatggccaa caagtatgtg ttatccaatg
      901 aagatgacca gttttgtgac ctggtgatca cgatggccag gatcgatcat ttggaaaagt
      961 agaaatctca tgggcatcag gccgtacacg taaaggtttt cctcagtgag cagaccggtg
     1021 tcaccggccg tgccatcgaa gagatcgcgg caagggtgaa gagggtaagg gattaacgtt
     1081 gggagaattt ctgttcacgt tcttttcggt ttgaacttgt tccgtagatg aatacaccaa
     1141 ccaggccgac aagcccggcg gtgccgagaa aggttcctgg gtaggggtgt cctttgatga
     1201 tgcagaaggc tccggaaaga acggtggtta ctccgatgag aaatccgcag atgatcccac
     1261 acaggctatc acggcttcgg cttcgaataa cgattttttc catttcgcgc ctgtgcatgg
     1321 actgttcttc ggccattttg aggattcttt ctgccgaacc tggaagaacg ctttcataac
     1381 cggaaaagtc tcccggtctt gggaggatac cggagtattc ttcgtgcctg atggcaagtc
     1441 cagagccttg ctgcttaccg ggtttcgtca tcgacgacct gcatggcgga cctgaggtcc
     1501 tgccccacga ttttccagtc ggatcggata gccatagcgt cggcttcttc gggtgtgcag
     1561 gattcgttga agatggtgag cgtgttgccc atatcaaggg ctgaggccat tccaccaaag
     1621 aatcccggca ttgcaaaaag ctggtcggta aaagacattt atttgatcct cctggttgct
     1681 attgagtctg aattttacca caaggtgtct taacggaaca agttaatcac gagtgtacgc
     1741 atggggaaca gactgatgta attcgaaaaa tgcaaccagt ggtatcaggc cgtacacgct
     1801 aaggttttcc tcagtgtggc cgagttattc gtaaaagtgc aaccggcggc gtcagacctg
     1861 cacaatttac aagacagaat gcgacaaaaa agcgcacata tgtctacctg caaaattgac
     1921 gttctcctga cgcgggtcct atactttcaa ggcaagcagg ggcgaaatag aagaaaacac
     1981 tattcatcaa cgcgtctggg gaggctgtat gtctgacatg gagctggcaa aacttacgga
     2041 acctggaata cgcaaagtcg cgggtgagcg aatcttctcg cgtggagaag catattacgc
     2101 cggaggcgct gttcacgaaa ctgttcgaga gggcagtgcg atccgcgccc tgtgcgaagg
     2161 ttcacgctct agcccctaca gggtgcgggc ggttttgagc gagagcggta tcgaaagtgc
     2221 ttcctgcacc tgtcccatgg acggtttctg taaacatatt gtagcgatgc ttctcaaatt
     2281 catagatgag ccagatgatt ttcgggaaat gccccctctt gaagatattc tttcttctct
     2341 gagcagggaa gagatggcct cagtgatctc aagcatggtt gaatgcgaac cacggctggt
     2401 tactcttgtg acgagagccg ccgccaaaac aggcgccgca aatgaccttg atggactgag
     2461 gcacgatatc agggaggcaa tgttcgggga tgatcccttc cagatcttga tgcggctcac
     2521 gggtattatg agtatggctc ataagcttgc ggaaaaagga gactggcatg gagccggatc
     2581 tgtgtaccat gagttattgc gggagcttgc gccagtttac gaagagtatc ttctcccact
     2641 cgatgatgat ggggatattg ccgccttagc ctgcgattgc gtagaggggc ttgaggtatc
     2701 tctgagggaa aaaggtattt caaaagaagc aaggtatgaa tggtttgagt cgctgctcga
     2761 tgcaatgctg gcggatatcc atatcggcgg cgtatccttt gctgaaggaa gtctcgatgt
     2821 gttgatggat tttgccggag aagaagactg gctttcgctg gagccgaccg tacttgacct
     2881 tatccccgca tccgagggct ggacgaggga agcattagtg aatgtccttg cagcctggag
     2941 gaaaaagtgg gtgaggagtg atgaagcccg cgaggtaatc cgcgagaagg gttcatcgag
     3001 gcaaaaggtg ttttccctgg tgcaggatgg caggtttgaa gaggcagtgt cattggcaag
     3061 agcccatctc gaagatcagc ccggcgtcat gctggatctg gctgacgcac tggtcgtggc
     3121 aaaagctcct gagtgcgctg tcgagcttct tttgcaatat gcaagttcgg aaaaacaggg
     3181 ctgctacagg tttttatgtt atcggcaatg gcttgccgaa tattacagaa aaagcagaaa
     3241 ccacaaaaag gctctggaat ggcagcggaa gctgtttttc gatgatcctt ctttagagat
     3301 gttttcggtg ctgcaaaagc tgggcaaaac gatgaaagtc tggcaggagg ttcgggcgga
     3361 agctcttgat tatttggaaa caataaagag gttcggtgtg cttatcgata ttgcccttta
     3421 cgaaggagat atccaaacgg ctcttgatct gctgcctcgg ttgcctcgtg cgagttgccc
     3481 ggatcccagg ctcgacgtag cacaagccgc cgagaagacc ctgcctctgg aagccataag
     3541 gctgtacaag gatcttgccg aaaaagccat cgctttgcgg catcgggaat cctacggaat
     3601 ggccgctcgt tatctggtgt gcgtcaagaa actgtatgag aaactggatc gcatcggaga
     3661 atggcgggaa gacctggctt ccttgaaaaa cagatataca cggcttcccg cactccagaa
     3721 tgaattaaaa aaagccggat tgtaaggtgg ggtgacagaa gatgtcttcg aatatcaaac
     3781 acaaatgggt ctttcgtccg cgcttcaggc gaggtgcgtt cggatggcga agccagcctg
     3841 ccatcacccg catcaaggag gctgtttcgg agatcaaaaa agtggcttca aaaaacccga
     3901 tccttggagg cgagggtgct gtcctttttc tggagaaggt ttccggggct cttgagaata
     3961 tcgacagttc ttcgggcgct attggaagtg ccgtcaactg ggcaatagaa tcactggttc
     4021 cgatcgttgc aaacgcgcct gcggatggtg cgcttcgtga gggatggctg gaacggctgt
     4081 ggagtgcggt gcaagcggac gatatgtcct atattgaatc gcttgccgat tattggggtg
     4141 agctctgcgc aacccctgag tgtgcctccc gctgggccga cagtttcatt gaaactgtca
     4201 gaatggtctg gggccctgga aaggaattgt caggttatta tcatggaatt ccggcctgtt
     4261 tgagctcgct tttcaaggcc ggaagaaacg acgaaataat ggatcttctc aagcaggccc
     4321 cttacaagac atggcaccaa agaatatggg gagtaaaagc tctttttgca acagggaaga
     4381 gaagcgaagc tatccgtttt gccgagggat caagggagtc gaatcaaccg gatttccgga
     4441 tcagcgaggc ttgcgaggag atactgctgg cgagcggcat ggtggaagag gcttatgaac
     4501 gctatgcgct tacggccaat cagaaaacca cgtatcttgc gacgttccgc gcaatcgcaa
     4561 aaaagtattc tcacaaagaa cccgcagtga tattgaaaga tcttgtggcc cgttctccgg
     4621 gcaataaggg taagtggttt gctgcggcta aatctgctgg cctgtatgag gaagctatca
     4681 gccttgcgaa tagttcgcca tgcgaccccg gaacccttgc acgcgcagcc agagatatgg
     4741 cagaaacccg gccattgttt gcaaaagatg cggggatagc agcgctgcgg tggcttgcgg
     4801 cagggtatgg ttatgagatc acgtcttatg atgtggtggc ggcttttgat catacggtga
     4861 aagcggcgaa aaaagcgggg tgcctgtccg aaaccattga aatgatccgg gaaatggttt
     4921 ccggggaaaa gccttgcgat cgcttggttt ccactatttt agagagagag ctgaaattga
     4981 tcgattaaga cagaaatggc ctcatttgat cactggagca acaggtggtg tcagacctgc
     5041 acaattgata tcttctggtt gttcgtttac ggagttcctt ttatccaatg tcagtgacaa
     5101 taaaagtgaa gttgtcagtg gccggagaag acatgatcaa atcggcgatc tctttggctg
     5161 cactaaaaat atcctttgtt tgaaggcaac gttcgagatt ttttatatcc aaatattccc
     5221 agacaccgtc agtgcataga aaaaagcgtt ctttttcctt aaggctctgg gaaaagctgg
     5281 agtacctggt cttcttttcg tttctgcctg agatggatga tgtaatgaca ttgcgcatag
     5341 ggtgggtcaa catctcttct cctgttatga ggccgctatc ggccatctcc ctgacgaggc
     5401 tatgatcctt tgttatctgc tgaaggaaag ggccgttctg ccggtagacg cgactgtctc
     5461 cgcatccgaa agccatgaaa cagttaaggg gaaggttgat gtaaagggca gccagagtgg
     5521 tggcaagccc ggataattcc ggtttcaggg ccgatatttc gataagtttt tccacagcct
     5581 tgtccaggac cggctcgggg tcttcgcaat tgagcagtga gatgttttcc agaatggctt
     5641 caagggcacc aaacgaagcc tctttacctc caggtcctcc ccccactccg tccgcaacgg
     5701 ccagaaatgg gacatcagct atctggcaga tcttcggctc caacatctcg ccgcagccga
     5761 tcagcctgga acccacgatg agagaatcct cgttaacctc cctgaccttg ccctttcctg
     5821 tcatgaacac agaagaaata atttccccgc ccccccttca ctgatcattt gttcgcttct
     5881 ttgagtcaca agaattctta atattttcat tggtaacctg tgaatgtttt tctggcacaa
     5941 aaccgaataa tgtaaatgcc ctgtcaaggg tttcgtcaat gggtgtccgg ttttaccctg
     6001 aggaagggtt tggttttaca tctgaatgta ttcttgatct ggcctatgca gatgttgtgg
     6061 tccgtaaaac ggatttccaa aatcaggaag aatcaggtcg gatagaggga aaaggatgcc
     6121 taaacactgc cagactgaag gaaatctacg ggaagatatg cgattctgag ggatattccc
     6181 ccaagttgaa gcatcagata cgaacaaatt tcaacaaggc aggtatttcc agacttccgg
     6241 aattgccagg gagaagaaac cgacgttaat cagaaaaatt tccactccca cagccatctg
     6301 tagacgatga tcctgttccc ggcgtagaag gcaacaaaca ttggcaagtc gaagaagaac
     6361 caccagacct tatagagatc ttcctttccg gcgaagctgc gttgaagtaa cgaataaaag
     6421 agataacaac caccatcgct ttacggcttg atagatattc ttttcccgtt gtgcacgcac
     6481 agtcttggag agggttaagt agattccccg tgcgggtatt ttgattttac aaactttgag
     6541 aatacttcag cagtttgcag cagttcttgc gggcggaagg gaaaataatc tctttcatga
     6601 tgatatctgg catcgacaaa atgatccttg ttcctttcta aaatattgag gatgtctgct
     6661 ataagttcgg aaagtgctga ttgtgtttct cttctgcatt gtccgaataa aaaaacaaga
     6721 tcatggtctt tgaattccct atctctttct atgaggatta tatatttaag gaaaagctcg
     6781 catgaaagag ctgaacatac tgtgccaggg acagtcaata tttccacgcc ttcttctatt
     6841 ttgcagccgt tgaggcaacg gtcagatgct ttgagaaaat tttctgcaga atccaaaaga
     6901 tcctttttca aatccgcgcg cagcttgatt acaccgccca aatagcttcc agggcctttt
     6961 cccgcaacgt caaagcttcc tagagagatg atgtctttgt tgttcttttg agaattatcc
     7021 ttgccggatc cgcaagaaca gcactctttc ttctcgggtt ccttctgcat ttcatgtacc
     7081 tctttccttt tcagaccgat gacacgttta caggtagtga agtagcagga gaccatctgt
     7141 ggtagagtcg gggcgattcc ttttgattcg cgcttcgggg ttctacggca ggcgcaagat
     7201 aatacagccg tgggtcgaat ggtttgatgg agtgagtaaa catcgatcct ataacctctt
     7261 tacaatccac actattttat gcgcatgctt tgcgtctttt ccgcttccat tcaggagcac
     7321 ggtttggaaa acggccttgc cttttgggag aacgtctaag caggaaacat acgcatcacc
     7381 aaccgctttt ttgaaaatat cgcaaaacag aattgaagcg gacaggcaaa tatattgatc
     7441 tgtcttattc acaaccacac cctttacttg gccattgttg ttgacctcaa catcatcgaa
     7501 gaaaatgcca ggagcaattt ctccgttatc gaaaaaagca aaggcgggca tggctatccc
     7561 caatacaagc gagataaaac aaacagcagc aatcattttt ttcatgattc aaactccctc
     7621 cccgttagaa attcctcttc aatctgacga ctcttccgca gatggtgaag tagccggaga
     7681 ccatttcggg cgggatgagc cgcggagatt ctttggggtt tgatcccctt agctctaccg
     7741 cgccggagcg gtggtagatg acgtgcttga gcatgggaga gccgtcaaag atgacgtaca
     7801 tgatccactc cggttttatg tcgtcgccct ggtacgggtt caccaggaca atgtcccctt
     7861 ccatgatccc gtcgccttcc agggaatgcc ccacggtttt gagcgcatag cttccctgtt
     7921 tcaccttgtc ttcgtcgaat gattccgtgc cgatgggttt cacatcgtcg ggacaccagc
     7981 cgtcggggcc gcagcccacg gatatgtcga acacaggaac ggatgtttgc gggggaagtg
     8041 caaactcgga aatagctgat tttagagttg tgggggaatt gatacccaaa atgtaatcaa
     8101 cagggacttg gaaaaaggaa gcaagggacg ctaacacacc agcatcggga gttcttttgg
     8161 aagtctcccg gttgatcacg gtgttacggg aaacccgcaa gttctctgcg ggttcttgcc
     8221 tagcaatcga cttatcttgc cttagctcct taatcctgat agcaactacc ccaatatgaa
     8281 ccacccctta attctatttt ccacgaatga aggcatcatg caagccccaa tatactttct
     8341 tatagattga taaacgtatg ctttcaggtt aacccctcag cacatagcgc acatgtatat
     8401 atgctgaagg gagtgaggtt tattggaaaa tcttgttcgg ggacatccag attcccgcac
     8461 taaggggaca cccactttcc cgcaggtgta tacactctct ctcttttggc acgttggtgt
     8521 gatcttaaat caaaaatgag taactccaga gtggccgagt tgaaccgaaa aacgtgcgac
     8581 gccggattac gaagccggaa gccccccaat actgaaaaac caaaggcagc ccccggatca
     8641 tccagggact gcctttttgt gtttttaatt tttcacgaaa gaattgatat tttctgatgc
     8701 gataaatttt atttagctca aaaggtcgtt tgcgtaatag gtaaggggga tcaggtcgct
     8761 ccggtcaaga tactccagtc tgaacttgcg gttcaaagcc gcgaaatgtc tcaggccaca
     8821 tcccagttta ttgagatagg agtaaacccc gatcgctccc gtggagaagt catctgcttc
     8881 ttttccatat attccacgga gatcggccag gtcatgaaaa acctcatcta tggtgctgcc
     8941 gaattttttg aaaagttcag gagtcctgcc tgcctttatt tcgtctccga ccgtcttccc
     9001 tgtcatggca gccgccattg cgccgcggca gacgcctacg gccgatataa atccatcgcc
     9061 gaaggcaagg gacttgaata tctggtcttc actgataaat ccgccggtca tgaccattgc
     9121 gggaagggtt ttgtcctggt cctgaagttt tgcacagata tcaacgacaa ctttttcaag
     9181 aacaactgtc gggaggcacc attcgttcat catcttggcc ggactatagc cggagccgcc
     9241 gcctgcgccg tcaaaggtaa tcatgtcgac cctgttctct atcgccatat tgatgacacg
     9301 ttcgacatcg atcacatcaa aaccggccat cttgaagtag acgtttttca taccaatggc
     9361 acgcagttcg gagatccgtt ctgccatgga ctcttccgtc cacattggca gccttccgta
     9421 agtgtaaaaa ttggggcagt agcccttttt cttaagttcg attatttttg gatcaaatgg
     9481 atcggggaag acaagatctc ccctctcgac tttttttacg gcctcatcat agtctttgat
     9541 cctgttcatc ggctgggtcc ccttggcacc ctggccgaac ttgaattcca gggcctcgaa
     9601 gccgtattta ttcagggcta tttcgggaac acctacaagg tggtcatcga cgttgcactg
     9661 aagcaccatc tgtcccggtc cccgatgata gttatagaaa ctgtcatgaa tcttcttgag
     9721 catggggaat gatgtgatct tgcccttttc tatcttgagt tcagggtcct tgttccttgc
     9781 gtcttcacct accatgcagg taactcccgc cattgcggcg ccgccgaaat aatcgaccca
     9841 gttcattttc accagggctg gcagaacaac gggaagatcc agcttgatta tattgtcggt
     9901 tccatattcg gaagccagat ctacgttgaa gatctctgcg tgctcatggt ccgcctctgt
     9961 tcccatggag ccaaaacacc gaccatttat gttgaaatga gaaaaatcta cggggtaatc
    10021 cttttccgac gcgatctggt tggctcccgt ggtcgtgggg tagaccgtca tcttcccaag
    10081 aaccgcagcc tgggttatct cacaggttcc cgggcattcc tcggtacata aagagcacat
    10141 tccagattga ggagaaatat acttgcttct gttttttgct cctgtaacac ctgaagtcat
    10201 acttggcgaa aacgtcataa ataaaccctc ctactaagga atttgtctta tgccccatct
    10261 gtttcccctg tctgatcggt gtcattgtac cacttgattt ttatcgggtt atacacagga
    10321 aacccggaag gtgcatgccc tccgggtaaa aatcctgttt gtggccttca ggggaaaatc
    10381 atgccgtcga atggttcttc tcatagttga agggtcggtc gggcaagatc cttatttcat
    10441 ctttcctctc agttcctgaa ggaagttcat gagatctttc agtccctggt tttttccgtc
    10501 tgaggcgagg gcttcgctga ccagggatat ctcctcgtcc agagtgaagg aggggttgaa
    10561 ctccagttcg atgttgaaag tggggtcaag gtagtaaaac ttccactgca gcctgtctgg
    10621 caggaaacgg taatcctctt cggggtggaa ccatctgatg acccctggtc ccttggcttc
    10681 gtgaaatacg tttatgactg cctccccgat gcttccgtac cagcctgaac ctgtcactag
    10741 tatgtatccc gtggaaaagg tctccattcc ggccaggagc ctgccgactg ccgagtcagc
    10801 cgacggttcc tggggcatgc ccgtcacgat cttttcgatg aagtccctgt atggtgttat
    10861 caggtagtcg tggttctttc gtggttttac cctgtaggtg accctcagtt ccgtctcggt
    10921 cttccccggg aaggtcatat cccagagggc ccagttgacg gttattccat cctcgtcgat
    10981 agactccggg tagaagagtt tttccttctc ctcgcggaaa gcaacgacat tgccgttgac
    11041 gaatacttcc aggccttcaa catcgtcacg gtagtcggtt gggaaaccca cctcgagggt
    11101 ggtctttttt tccgaaaggt tggcgaatct gaagatcgct tccaccgata cggtctcttc
    11161 cccgagatgt atgttgacct cctcatcggc catccatacc cggtgagtcc tggccgatgg
    11221 agccagtccc gagttcagtg gatccggggc cgcatctccg aaggcagttc ctgccgcggc
    11281 cagcgctata agaattcctg ttgccaggta tctgattatc ttcaaaatcg taaccccccc
    11341 gatactttat tgactacctg tagtataact gcttgctacc cgtttttccc ggatcgaggc
    11401 cttgccgccg ggtggttttt tatgataccc cgtgacatcc gcccctcttc tggtgtataa
    11461 atagtaaaca taaaacgtct ttggtgactt ttggcaggtg tttttaagtg aaattcgccc
    11521 tctgtgactg caataatttt tatgcctcct gtgaaagggt tttccgcccc gacctcagag
    11581 acaggcccgt tgtcgttttg tccaacaacg acggttgcat tattgcccgc tcggcggagg
    11641 tcaaggctat gggcatcaaa atgggtgcac cctattttaa agagaggtac cgcctcgaaa
    11701 gtgccggagc agcggttttt tcttccaact atgcacttta cggggatatg tcgagaaggg
    11761 ttatggagac gctggggacc atggttccgg atgtggagat atattctgtc gacgaggctt
    11821 tcctgttgct ggattcggct cctttgcggg gcagcacggc cgaggagttg tgtcgccatg
    11881 tcaaaaaaca ggtgttccgg tggacgggga tccccgtgtc cctcggcctg ggtgccacaa
    11941 aaacccttgc caaggcagcc aacaggatag ccaaaaaaga gagttcctgc ggaggtgtct
    12001 tcgcgatacc tgagggtgtc ggagcagacg tttttcttgc cggtttgaat gtggaagatg
    12061 tatggggtgt gggtttccgc tacgccccca tgctcaaaag gagcggcata agaacggcac
    12121 tggatctcaa gatggccgac gacgaatgga taaggaggcg tatgaccgta tgcggccttc
    12181 gaacagtgtg ggaactcaga ggtaccgttt gccacccgtt cagggcctgt ccggatccgg
    12241 caaagagcat aaggtcatcc cggagctttg gagagcccgt gatttccctg catcatttgc
    12301 gtgaggccgt ggcgtctttc gcggaacgcg catgcgagac tctccgaagc cagtgttcta
    12361 cagccggatg tatatctgta ttcatagcta caagtcccca cagggaagat cctcaatatg
    12421 ccaacgctgc cacaaggagg cttgccatgg actccgattt tacaccggcc atcattaccg
    12481 ctgccatgga atgcctcgag gagatttatg tgccaggtct tcagtacgtc aaggctggtg
    12541 tactccttcg cgaactgcgc tctgccctga cgcgccagag cggtctcttt gacgtagcgg
    12601 agacgggtcg gagagtcagg ctcatggcgg caatagaccg gatcaatggc gatctcgggc
    12661 gcggaagaat atctgttgcg gcctcagggc gatggaagcc ccgtgtatgg gacatgaagc
    12721 aggatctcgt atctttttgt tacacgacca ggtgggagga tctcgccggg gtccgcgtgg
    12781 tcgggggaga ggtgccgccg tgcaggaact gaagatcacg gggttcccgt ctccggcggc
    12841 ttcctggtcg gaaaaacctc tggatatcct gaatctgctc gcccccaacc ctgtgtccac
    12901 atttttcatg cgcctggagg aagatgtccc ccaatgggct cttctggagg gggacatcct
    12961 tgtcgtagac cggtcactgg aacctcgcac aggaagtctt gccgttgtgg ttgagaatgg
    13021 cgccctggtc ttgagggtca tagggcgctc gggtgcccgc agatccttcg cgttgcccga
    13081 agggaaagaa agcagcggta tcgatatcga aacctggggg atcgtcaggg gtgtggcgag
    13141 actgtggtag gggggcttgc tggtcatttt ctgaaaacga gaaaacgcct tgatgtggtt
    13201 tccagccgaa atcctctctt cacggccgct tctaccaggc gggctttgtt tcccctgcca
    13261 gggcgatagg ccattgtgcc gccgggtttt acgactcgac cgagttcatc cataaggtta
    13321 tgctccccgc ccacaacgtg cgggaccccg acaacaaaag cgaaatctat ggttctatcg
    13381 ccgagtcccg aatccgcagc gttgtggttt tccacccgga cttgccccag tgagagtttc
    13441 tgaacctttt cccgagcaaa tttgcaggca taggggttta cgtcgaaaga tacgacctcc
    13501 cccgatttgc cgacggcttt tgccgctccc acagtataga acccgggtcc gcaacctggc
    13561 tcgaagaccc gatccccttc cctgagcccg aggttcctga gggcctccgc ggggtgaaca
    13621 aatttttcga gaagcctgtt atcgtgcatg agccgtattg tgacgaaaga gaaccactgc
    13681 gcaaaattcc tgctgctttc tgatctgttt ttcattcttc tgcctccttg tttgatatca
    13741 gggtgcgtac gtgcctggct cgtctcatca gaagatcgat aactttttcc accatggcgg
    13801 attcctctct ggaaaatctg gcaagttcat taatgtacgg agtttctgcc cgcatgtgaa
    13861 aatccaggtg tgcctcaaag gcccgttctc cgcggtcggt gagttcgaac agaacctctt
    13921 tgcggttttc aggtacctgg tatctccgaa ccagcccccg ccgctgcagt tttgcaatag
    13981 cctgggaaac agcacttttg ctgatgctgt gtttgcgggc cagtccggtg acgttgattc
    14041 cccggttttt cccgatggtg tggatgatat ggatgtccag gttatgaaaa agatctccgg
    14101 taccatagtg tttcggcatg gccgcaagct ggacgtattc ttcggtcatc cagagaattt
    14161 tttcgatcat ttccagggcc tttttttctc cgatcaagaa ttggaacctc ccccctaaag
    14221 gatcaagttc gtttacgtgc ttaacaaaaa tggtagatca tgttttttat tttgtcaacc
    14281 aggtttacaa aatattttat gttcaccggt gacctgttcc ggctccgctt ttgcagatta
    14341 tcgcaattgt aattcgcttt gatctctgta tgtcaaagta ataaaatata aatacaggtg
    14401 ccggtttagg cgaactcgca tacgcatacc ggagaatgtt tgagtgtgcc ggttctgtaa
    14461 cctgtcgatg attaccctgt cttgaggatt tgcgacatac agaagtattt acaagcccgg
    14521 ataaaagtgt aaaatcaata ccgaaatacc tgaatatgga tcaaaaagtt tttctctggg
    14581 gggctgcgga atgtattgtt ttcagtgcca ggaaacagca aagaatcagg gttgtacggt
    14641 aaggggcgta tgcgggaagc cggaggggac tgccgatctc caggatcttc tcatctatgt
    14701 atgcaaggga atatccgtct tcggtgaaga gttgagaaaa tccggtaccg tcgacaggga
    14761 tgcggggcga ttcatctgca gggcgctttt cgtgacaata accaatgctg cctgggatga
    14821 tgattccata atcgaatgga taagggaagg gctcagggtt aaagaagcca ttgccggcag
    14881 ggtcggcgat ctcccgggga aattgccgga ttgcgctacc tggaaagcgg acagcaagga
    14941 agagattatg accaagtcca tgtcggacga ggtacgcgta acggctacag aaaatgatga
    15001 cgtccgttcc cttagggaac tggtcatcat agggagcaag ggtgttgccg cctatgccga
    15061 acacgctgaa gttctaggtt tcgaaaagga cgagatatat gaggcgatta tggaaggtct
    15121 tgcgtccaca acgaaggatc tttccctcga cgagatggtt gcgatggtaa tgaagacggg
    15181 tgagaatgcc gtcaccacaa tggctcttct tgacgaggcc aacacgagta cctacggcag
    15241 tcccgagatc agcgaagtca atctcggtgt caggaacaat ccaggcattc tgatttctgg
    15301 acacgatctt aaggacatgg atgaacttct aaaacagaca gagggaaccg gtgtcgacat
    15361 ctatacccat ggcgaaatgc ttcccgccaa ttattatcct gctttcaaga agttcgaaca
    15421 tttcgtgggc aactacggtg gttcctggtg gcaccagaac gaggagttcg agtccttcaa
    15481 cggccctgtt ctgcttacga ccaactgtct tattcccttg aaaaaggaaa acacctacct
    15541 tgacaggctt ttcaccacgg gtgtggttag ttatcccggc gccaagcata ttcctgacag
    15601 gccgaagggc ggaaagaagg atttttccgg gcttgtgaaa atggcgaaaa agtgtgctcc
    15661 gcccgaggaa cttgaaaagg gcaaaattgt gggagggttc gcccacaacc aggtaatggc
    15721 cctggcagac aaggtcgttg aggccgtcaa gtccggtgcc atcagtcgct ttgtcgtaat
    15781 ggcggggtgc gacggccgct tcagatccag agattattac acggaggttg ccaggaacct
    15841 gcccagagac actgtcattc ttacggcagg ttgtgccaaa tatcgttaca acaagcttga
    15901 tctaggcgat atcggtggaa tacccaggat acttgacgcg ggccagtgca acgattccta
    15961 ctcccttgcg gtcattgcca tgaaactgaa agaggccttc gggtttgccg atattaacga
    16021 actgcccatc tcctacgata tagcctggta cgagcagaaa gccgtggccg tactgttggc
    16081 cctgctttct ctgggcataa aggggatccg gctgggaccg acactgccgg cgttcctctc
    16141 gcccaacgtg gtaaaggtgt tggtggataa atttgacatc aagcccattg gcaccgtcga
    16201 agaagacatt accgcaatga tggcgggcaa gtagttttat ttttcaatga acggtgtttc
    16261 ccgattattc ccacctcttc atttctgccc tgagcatcca gaaggcaagc agaaagcctg
    16321 tcaggtccat gaaggggaag gagagccata caccgtcg
//
LOCUS       DAOTIQ010000042        16030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4393_length_16030_cov_6.862535, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000042 DAOTIQ010000000
VERSION     DAOTIQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_16030_cov_6.862535"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="P5541_06430"
     CDS             complement(<1..513)
                     /locus_tag="P5541_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV71656.1"
                     /translation="MLKVARERELIRLCQEFVRLPSTSGNERQMAFFVRDTMLAMGFN
                     RADVDPYGNVTGSVVLGEEGKCLLFESQMDHVDVADPFEWDLYPFGGFKRNGKIYGRG
                     ATDQKGSLAAMICAASFLREDAPAGCSGKIVVAATVHQERAEGASSRLVDINHRPDLV
                     VSGEASALAIE"
     gene            638..1552
                     /locus_tag="P5541_06435"
     CDS             638..1552
                     /locus_tag="P5541_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRV71657.1"
                     /translation="MITLFQLSSFCCVVEEGSFSAAAERLFVSQPSVSQHISALESHF
                     GASLFNRQKRKISLTPEGRLLYSSAREILDGIEDVGQRISNLKSIHTGDLKTGFSPYA
                     ASCIAPRVISRFTAKFSAVRISLVTGDMVDLEKKISDNDLEMAITERKLNHNMDPSMN
                     SHTLGYEDLVFAASPNLYISSIPAGPRDLELLPAITCTGNNPLGPYLDDFALQNQIRF
                     NRRIQTDNPETAGLLAAEGLGFALLTRSFAEAEQKEGRLRIIELDTAPPARLEILAIY
                     HRTRGLTYPGWEMLKLLENEVSPPLKEG"
     gene            1672..2115
                     /locus_tag="P5541_06440"
     CDS             1672..2115
                     /locus_tag="P5541_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase"
                     /protein_id="HRV71658.1"
                     /translation="MGKESIAQKIREKLKDIPEEFKDMKEAYCLDTVIAERKAFLSKQ
                     RLSYIARFKVDETAKEVTFTERLKESRSGLGAGDPDDTGPGLSFIKNTVSSGVDGLEG
                     IVDQQSTLFGKKYDYSFDHKLVRSTVREVATSEGYSFTYKIWGKL"
     gene            2159..2542
                     /locus_tag="P5541_06445"
     CDS             2159..2542
                     /locus_tag="P5541_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71659.1"
                     /translation="MRKEILMRSLFVFAVLAIFAVSAYAVTTHTVYARVVSGTGAIQA
                     VLEPGEFTVPSGSTAVIKKFQHDNPATGYHNEKLGKNIYSVTQGIYMVDGNGNALMQL
                     PAGQYRFVVGGSPRATGSLVYELHP"
     gene            2632..3018
                     /locus_tag="P5541_06450"
     CDS             2632..3018
                     /locus_tag="P5541_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71660.1"
                     /translation="MRKTRFSIGVLAFFALFAMAVSVCAVSGPYTLHATVVSGQESVH
                     AARKGAEFLVPPGSTAVITHYWWHDPGSGQTSDRLRSNIFSVEKAIYMVDNNGDGIRQ
                     LPPGTYRFIIGGPVGATGKLVYELHP"
     gene            complement(3096..3818)
                     /locus_tag="P5541_06455"
     CDS             complement(3096..3818)
                     /locus_tag="P5541_06455"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71661.1"
                     /translation="MITINSKGKSNSLRFVVYDFFRDEMQKGNLIPGSMINLKLISES
                     LGISMTPLREALVQMETEGFVSIIPRRGVVLNGLSLRKIRNIYSILGALEAVALRETF
                     PQIDETVVERARKNNEEMRKCLEENDPRGYLARNNRFHGIWIDRTDNEELVRQIKVMK
                     ERLYEFPSNEDLIHEWEESSLREHEEILRLVERGDVENTANYLQFVHWSYENQEHFIK
                     RFYSRHLESLEKKRGAGIYAPG"
     gene            4095..5210
                     /locus_tag="P5541_06460"
     CDS             4095..5210
                     /locus_tag="P5541_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRV71662.1"
                     /translation="MRKLFVCFLILALSLSVASAALAATKVGVMTGTTSQGEEEYQAA
                     QEMIKKYPGRIIHVTYPDKFMDEQETTIQQILSMAADPDVKAIALVQGVPGSAAAIDK
                     VKEIRPDMFFINIVPHEDPMLSAQKADIVLETDNPRRGVTIVELADKMGAKTFCHISF
                     PRHMSYPLLSERRDIMKAECEKRGIEWVFVNAPDPTGDAGIAGAQQFILEDVPRQVEK
                     YGKDTAFFSTNCGMQEPLIRSVLTTGAIYPEQCCPSPYHGYPGALGIEIPEGKAGDVA
                     FILDAITEKVVAGGGAGRFATWKIPINMAFTYAAVDYGFDIADGKVKGVDIEHMKKLL
                     TKHAGNAQFSTYNNVATGEKADNFLLVVGESIIFGGK"
     gene            5325..6923
                     /locus_tag="P5541_06465"
     CDS             5325..6923
                     /locus_tag="P5541_06465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV71663.1"
                     /translation="MTGPRLEMKNIRKEYFGNKVLKGINITVEPGEIHAIVGENGAGK
                     STLMNVLFGMPVIHSTGGFKGKILFNGKPAPSSSPEDAMALGIGMVHQEFMLLPGFTV
                     MENIKLNREPTIPNIWSKFLGEKMKTLDIQKMRNDARASLEKLDLGIDEMLRVEGLPV
                     GFMQFIEIARELDKDNLQLLVLDEPTAVLTEADADTLLETVKRLSKQGISVLFITHRL
                     DEVLKIADKITVLRDGEQVATMKPEEATVGKLAELMVGRKIQAVTGKDKCETDMECPE
                     PILSIRNLHVEMPGEELHGVDLDVTPGEILGIGGLAGHGKIGIANGIMGLYPSTGKIV
                     LNSKPVPLNNPIEALNSGMAFVSEDRRGIGLLLEDSIEMNIIVQAMQSQGKFLKKGII
                     QLMDFATSRQHTEKMIKDLDIRCTGPDQITRRLSGGNQQKVCIAKALTLEPRVLFVSE
                     PTRGIDVGAKRLVLNLLVELNRKAGVTIVMTSSELAELRSVCDRIAIIYKGRLEGILP
                     PDDSNERFGLMMAGALTHHMKEVS"
     gene            6923..8008
                     /locus_tag="P5541_06470"
     CDS             6923..8008
                     /locus_tag="P5541_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71664.1"
                     /translation="MPEDSLVTEKTTLKERALDFYRDFGVARLIIFAFLVFLLASALF
                     LKLPMEQILSSVLTRFGMNGILVLAMIPSIQSGAGPNFALPLGITCGIVAATLSIELD
                     MAGFSGLFFSIGLAAILGAITGFFYGVLLNKVKGQEMTVGTYFGFSIVSLMCIFWFLA
                     PYKSPEMIWPYGGSGLRVTIAIQDRLGKVLDNFLSFDILGVTIPTGLLLMFGLFSLAL
                     WTFSRTKTGIALSTVGSNPRFAESSGLNVNRYRIIGSSISHALGAVGIVIYAQSFGFI
                     QLYMAPLFMAFFAVASILIGGASLKRATVTHAIIGTFLFQSILVVSMPVANVVVADNI
                     ADVIRIIISNGIILYALTRREIGGDVS"
     gene            8041..9144
                     /locus_tag="P5541_06475"
     CDS             8041..9144
                     /locus_tag="P5541_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71665.1"
                     /translation="MLQEIEETTYKERIWKYYLRKNVVPVFFLVLCVFGGYYSGLSLP
                     FLINEIITRMARNSFLVISLIIPILAGMGLNFGIVLGAMAGQFGYIATANFQMAGFSG
                     FAVTTLISIPLSILFGWLTGKLFNQAKGKEMITGIILGFFANGIYQLICLYFLGNFIP
                     IRVPTMLLSTGIGLRNTLDIKSMHYALDNLWKIRINIPGFIFPIIIPVFTLLAVALLC
                     LAIHFLIRTKLGQEFRAVGQNRHIAEVAGIKVDRTRVIAIIFSTVLAGIGQVIFLQNL
                     GNINTYGSHVQVGTFAVAAILIGGASVSKATIGQALLGTLLFHTLFIVSPLAGKNLIG
                     DAMIGEYFRVFVTYGVIGLALALHAWQARQKRR"
     gene            9160..10260
                     /locus_tag="P5541_06480"
     CDS             9160..10260
                     /locus_tag="P5541_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRV71666.1"
                     /translation="MAANSRFSNAFDLVRKNGYDAFVMAPSADLEYLTGLSPHPGERF
                     NGLFILADGRSFAVVSRLYLQEYQQTLPGSLTLYDWGDEEWFFPALKKAFSDHDIAEG
                     AVIALNDSIMAVDAVEIAERQKVRLANGWHTLEPLRLIKDKEEIACLKKAGDIADKAL
                     KSLLQFIRPGVSEKTLRRKLLDLLDDHGATSQAFTPIVARGENAAMPHYPGGDGVIRE
                     KDSLLIDFGCRYRGYYSDMTRTIFIGEPDHETRELYDIVLRSQDLGEKTARPGITAED
                     LDISARSVIEEAGYGDFFNNRLGHGIGIAIHESPNIMRGNQMTLAKGMAFSIEPGIYI
                     PGRIGIRIENCVVLTENGCEPLTLFPKDLLVV"
     gene            10274..11650
                     /locus_tag="P5541_06485"
     CDS             10274..11650
                     /locus_tag="P5541_06485"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRV71667.1"
                     /translation="MEYEAMLERYRPRIISNLQEMIRIRSVEAPAVDGKPFGEGVDQA
                     LRLALETASILGLRAVNRNGMIGYAEYGSGKEMVAVLAHLDVVPEGKGWTFPPFGAEI
                     HDRKMYGRGTTDDKGPAIAALWALKFLAEENHRLSKRIRIIFGTNEESGFRGIKWYAE
                     NEEIPVAGFTPDIHEGIVFAEKGGLHVQMDIPVNREGRVFLEDFRSGEAVNIVPDLAE
                     AVFSGEISELDSWADALESQKGYLPLGGEVIREKERLIIRAKGKAAHASLPENGINAI
                     AALCCFLGRSPEPGIFDTLSQLFRDPAFGKELGIEMTDEVSGPLTCNPGLASFSEGTL
                     SITLDIRHPVTFTGDHVEEKIRGILGPLGINIRVTSRANPLHVPKDSQLIKDLSDVFF
                     EVTGKRPELKAMGGGTYAKALPNVVAFAPFPNGLPELAHQADEYIDIEEFLASILVIK
                     NALIRLAR"
     gene            12166..12996
                     /locus_tag="P5541_06490"
     CDS             12166..12996
                     /locus_tag="P5541_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV71668.1"
                     /translation="MEEARLLRLKTMSNLIRKDIVKMVLDAGSGHPGGSLSVADIVSV
                     LFFDVMTHDPSNPCWEGRDRFILSKGHACPAVYAALAEGGYFPREELNTLRKFGSRLQ
                     GHPDMCKLPGLEASTGSLGQGLSIASGLALAMKIDGKSNRVYCLMGDGELDEGQIWEA
                     AMTASHFGLDNLCGIVDRNGLQIDGRTDDIKTLEPLADKWRAFGWNVIEIDGHNVTEI
                     SGAMNRFRHTHFRPTVLIAHTIKGKGVSFMENQAGWHGKAPSKEQAEAALMELSQEVV
                     "
     gene            12997..13977
                     /locus_tag="P5541_06495"
     CDS             12997..13977
                     /locus_tag="P5541_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV71669.1"
                     /translation="MKDLHLAKNMLDEPERIPTRFGYGDGLVELGKTNPSVVVLGADL
                     SSSLCVDRFRDAFPDRFIQTGIAEQDMMSFAAGLSLAGKIPYVSTYGVFCTGRAWDQL
                     RTTVAYARLNVKVGAGHGGVSVGADGATHQALEDISITRTIPNVTVIVPADYNETVKA
                     TIHSALIRGPVVIRFGREKVPVVTTKETPFTVGRAEIFHEGEDVSLIACGQMVYESMV
                     AAEILAKKGVSARVINCHTVKPMDRETILAAARETGAIVTAEEHQVMGGFGSAIAEVV
                     CQSCPVPIRMIGIQDTFGQSGDPEELMKAYGLTGQDIAKTALDAIAMKKG"
     gene            13984..15180
                     /locus_tag="P5541_06500"
     CDS             13984..15180
                     /locus_tag="P5541_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV71670.1"
                     /translation="MSISFAERTKRLGTETAFAVGAEAAQLASTGVKVFPFHLGDLNI
                     PTPEHIAEASSKAIKDGKTGYCPTPGILPLREALAADISKSRNLDLTADNVSIQTGGK
                     PVIGKFIMSLMNPGDEVLYPNPGYPIYESMINFNEGIGKPYGFIETATGFALDMDAIE
                     RQITPRTKLLIYNNYHNPTSAESDENEMEKLADICVKNDLYVLSDEAYFDMLYEGTGK
                     SITRFPGLFERTVILYTFSKKFAMTGWRLGAAIGPKPIIDAISLFNVNDESCTNNFVQ
                     WAGLAAITGSMEPYKKMIGILRERRDAAVEMLNKMNGVSVSTPDSTFYLFPNVTGAMR
                     KLGLTDVEEFRKLVLHKTGVSFTTRDHFGTPLPGETQKYIRLAYSGINTPEIIEGLGK
                     MKELLG"
     gene            complement(15725..15839)
                     /gene="rrf"
                     /locus_tag="P5541_06505"
     rRNA            complement(15725..15839)
                     /gene="rrf"
                     /locus_tag="P5541_06505"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         4051 a         4159 c         4046 g         3774 t
ORIGIN      
        1 ctctattgca agggcggaag cctctccgga aacaacgaga tcagggcggt ggttaatatc
       61 tacaagcctg gaagaggctc cctcggcgcg ctcctggtga accgtggcag caactacgat
      121 cttgccactg caccccgccg gagcatcctc tctgagaaaa gacgccgcgc agatcatggc
      181 ggccaggctg cctttctggt cagttgcacc tcttccgtag atcttgccat tccttttgaa
      241 tccgccgaag gggtacaaat cccattcaaa tgggtcggca acatcgacat gatccatctg
      301 ggactcgaaa aggaggcatt tcccctcttc cccaaggacg acacttcctg taacgttgcc
      361 gtagggatcc acgtcggccc ggttgaagcc cattgcaagc atggtgtccc tgacaaagaa
      421 agccatttgc ctttcgtttc cggaggtgct ggggagccgg acaaattcct gacaaagcct
      481 gataagttcc ctttccctgg ccaccttgag cataaccgca cctgtttaga cagaatcttg
      541 ctttttactt ttttcattgt aatgttcttt ctattattat caataaagga tgtttgatat
      601 tagtgatagg ttttgtctta tgaaaggagg tgtaaagttg ataacccttt tccagttatc
      661 atccttctgt tgtgttgtcg aggagggatc cttcagtgct gcagcggaga ggctttttgt
      721 ctcacagcct tccgtcagcc aacacatttc tgcacttgaa tcacattttg gagcaagcct
      781 cttcaacagg cagaaacgaa agatcagcct gactccggag gggcgcctgc tttattcttc
      841 ggccagagaa attctggacg gaatagagga cgtaggacag agaatatcga accttaaatc
      901 cattcacacg ggggacttga aaacaggctt ctcgccttat gctgcatcct gtatcgctcc
      961 ccgggttatc tcaaggttta ctgccaaatt ctccgccgtg aggatatcac tagttactgg
     1021 cgacatggtc gaccttgaaa aaaagatcag tgacaacgac ctcgaaatgg caataacaga
     1081 aagaaaactc aaccacaaca tggatccgtc tatgaactct cacaccctgg gctacgagga
     1141 cctcgttttc gcggcctccc caaatcttta tatatcatcc atccctgccg gaccgcggga
     1201 tctggaactg cttccggcga ttacctgcac aggaaacaac cctctggggc cctatcttga
     1261 cgatttcgct ctccagaacc agatacgatt caacagaagg atccagaccg ataacccgga
     1321 gacagcgggc cttcttgccg ctgaaggact gggctttgcg ctccttacaa ggtctttcgc
     1381 ggaagcagag caaaaagaag ggcgcctgag gataatcgaa ctggatacag ccccgccggc
     1441 ccgcctggaa atcctggcaa tataccacag gacccgcggt cttacttatc ccgggtggga
     1501 aatgctgaaa ctgctggaaa atgaagtgtc acctcccctc aaagaaggct gagatgttcc
     1561 cggccaaggg accctgcatt tcttttttcc tttatcgtta tgacccgttg cgctgctaaa
     1621 ccaaaaggaa tataattccc aattatcaga cccgggaaga ggaggatagc catggggaaa
     1681 gaaagtatag cccaaaagat ccgtgaaaaa ctcaaggata ttcccgaaga gttcaaggat
     1741 atgaaagagg cctattgtct tgacacggtg atcgcagaaa gaaaggcgtt cctttcgaaa
     1801 cagcgacttt cctacatcgc gcgcttcaaa gtcgatgaaa ccgctaaaga ggtaaccttt
     1861 accgagaggc tcaaggagag cagatccggc ctgggggcag gagaccccga tgacaccggt
     1921 cccggtctca gttttataaa gaacaccgtt tcttccgggg ttgacggact ggaaggcata
     1981 gtcgaccagc aatccacact gttcggaaag aagtatgatt acagtttcga ccataaactg
     2041 gtacgatcca ctgtaaggga ggtggcaacg tcagaagggt attcctttac ctacaaaata
     2101 tggggaaagt tataaaaaag aacctgtaaa acgatctcga taataaggag cgggaacaat
     2161 gaggaaagaa attctgatga ggtcactttt cgtgttcgcc gtgcttgcga tctttgcagt
     2221 ttccgcttac gcggttacga cacataccgt gtacgcaaga gtggtatccg gaaccggggc
     2281 catacaggcc gttctcgaac ctggtgaatt cactgtccct tccggctcca ctgcggtaat
     2341 caaaaagttc cagcacgaca accccgctac aggctatcac aacgaaaaac tcggaaagaa
     2401 tatctactcc gtcacacagg gtatctacat ggtggacggc aacggaaatg cccttatgca
     2461 gcttccggca gggcagtaca gattcgtagt tggaggttca cccagagcca cgggctctct
     2521 ggtctacgaa ctgcatccgt agggaatccg tagggagatt gacaacacct ttaaccagtt
     2581 ttatgattta cccgtgtgtt gttacatcta tctgaaggga gagaaaaggc gatgagaaaa
     2641 accaggtttt ctattggagt ccttgccttc ttcgctcttt tcgccatggc tgtttcagtc
     2701 tgtgcggtat cgggacctta cacccttcac gctactgtcg tcagcggcca ggaatctgta
     2761 cacgctgcga ggaagggcgc tgaattcctt gtgccaccgg gttctaccgc ggttattacc
     2821 cattactggt ggcatgaccc aggctcagga cagacaagcg acaggctccg gagcaacatt
     2881 ttttcagttg aaaaggctat ctacatggtc gacaacaatg gtgatggcat aagacaacta
     2941 cctccgggaa cctatcggtt cataatcggg gggccagtag gcgccacagg caaactggtc
     3001 tacgaactgc atccgtagtc actttatctt acaaggggaa agagaaaggg ggggcaaggg
     3061 agaaagaccc tcacctcccc tttttaacac ggggattatc cgggagcgta aattcctgct
     3121 cctctttttt tttcaagtga ttccaggtgc cgggaataga agcgcttgat aaaatgctcc
     3181 tgattctcat aactccagtg aacgaactgg agataattgg ccgtattttc tacatctcct
     3241 ctttcgacaa gcctgaggat ctcttcgtgc tcacggagac tggattcttc ccattcgtgg
     3301 atcaggtctt cgtttgacgg gaattcatac agcctttctt tcatgacttt tatctgcctc
     3361 acaagttcct cgttgtcggt ccggtcgatc catatcccgt ggaacctgtt gttccttgcg
     3421 aggtagcctc ttggatcatt ttcttcgaga cattttctca tctcttcgtt gttcttcctg
     3481 gcacgttcca ctactgtttc atcaatttga ggaaaggttt ccctgagagc tacagcttcg
     3541 agcgccccga gtatggaata aatatttctt atctttctga gggaaagccc gttgagaaca
     3601 acccctcgcc tggggattat agagacgaaa ccttcggtct ccatctgaac cagagcttct
     3661 ctaaggggtg tcatgcttat ccccaggctc tcgcttatta atttgaggtt gatcattgat
     3721 ccgggaatga ggttgccttt ctgcatctcg tcacggaaaa aatcgtagac tacaaatctt
     3781 agtgaattac ttttcccttt actgtttatt gtgatcatga tctgatcctc ctcaactttt
     3841 cccgtgaaaa gccttgactc tctgataccc ttctggtaca taatatatga gaaatatatt
     3901 ttaaatatat ttctttgtcc acctttgaaa tgtttcattt tttctttctg aagagtttat
     3961 caaattcagt caagggggcg atgccggggc agaaaatctg ggtgtcgtta ggattgaccg
     4021 tttttagggt ccctctccca caatttattt cgggaaaagg ggtaatttct tcattgaagg
     4081 aggagtgaaa caccatgaga aaactgtttg tgtgtttttt gattctcgct ctttctcttt
     4141 ctgtagcgtc tgccgctctg gccgcaacca aggtcggtgt tatgacaggc accacgtccc
     4201 aaggtgaaga ggaataccag gctgcccagg aaatgatcaa gaaataccct ggaaggatta
     4261 tccacgtaac ctaccccgac aagttcatgg atgagcagga gaccacgatc cagcagatcc
     4321 tcagcatggc cgccgatcct gacgttaagg ccatagctct tgtacagggt gtccctggca
     4381 gcgctgccgc tatcgacaag gtcaaggaga tccgtcccga catgttcttc atcaacatag
     4441 ttccccatga ggatcccatg ctctcagccc agaaggctga catagtactt gaaacagata
     4501 atccgaggcg aggggtcacc atcgttgaac tcgcagacaa aatgggagcc aagactttct
     4561 gtcatatttc cttcccacgt cacatgtcct acccactgct ctcggagaga cgcgacatca
     4621 tgaaagcgga gtgcgaaaag cgcggcatcg aatgggtctt tgttaacgct ccggacccca
     4681 ccggtgacgc aggaatagca ggcgcacagc aattcatcct tgaagacgtt ccccgccagg
     4741 tcgagaagta cggcaaggac acggccttct tcagcaccaa ttgcggcatg caggaaccac
     4801 ttataaggtc ggtccttact accggggcca tctatcccga acagtgctgc ccaagcccgt
     4861 atcacggtta tccgggagct ctcggtattg agatccccga gggcaaggcc ggagacgtgg
     4921 ctttcatcct tgatgccatc actgaaaagg tcgtcgcagg aggcggtgcc ggaaggttcg
     4981 ccacatggaa aatacctatc aatatggcct tcacctacgc agctgtggat tacggctttg
     5041 acattgccga cgggaaggtc aagggcgttg atatcgaaca catgaagaaa ctgctcacca
     5101 aacacgccgg caatgcccag ttcagcactt acaacaatgt tgcaaccggg gagaaagccg
     5161 acaacttcct tctcgtagtg ggcgagtcca tcatcttcgg cgggaaatag cgcaacgagc
     5221 atctacatca atccatttga ataaaaccaa aaaagagaaa gacggggcct cgtaacttct
     5281 ggcccctctt tctctgtcaa aattgaaaat aaggaggatt ccggatgacg gggcctcgtc
     5341 ttgaaatgaa aaatatccgc aaagagtatt tcgggaacaa agttctcaag gggatcaaca
     5401 taaccgttga accgggagag atccatgcca ttgttggaga gaacggagcc ggtaaatcaa
     5461 cccttatgaa tgtccttttc ggaatgcccg tgatccatag cacagggggg ttcaagggga
     5521 agatcctttt caacggcaag ccggcgccct cttcttctcc ggaagatgcc atggcactgg
     5581 gcattgggat ggtacaccag gaattcatgc tgcttcccgg tttcaccgtc atggagaaca
     5641 tcaaactgaa ccgggaaccg accataccca acatctggag caaattcctg ggtgaaaaga
     5701 tgaagaccct agacatccag aagatgcgga acgatgcaag ggcctccctt gaaaaacttg
     5761 acctcggtat agacgaaatg ctcagggtgg agggccttcc agtgggattc atgcagttca
     5821 tagagatagc acgcgaactc gacaaggaca acctgcaact cctggttctg gacgaaccga
     5881 cagcagttct tacggaggct gacgctgaca cccttcttga aactgtaaaa cgcctttcaa
     5941 aacaggggat atctgttctt ttcattactc acaggcttga cgaggtcctc aagatagccg
     6001 acaagataac cgttctgcgt gacggtgagc aagtagcaac catgaagccg gaagaggcca
     6061 cagtcggaaa acttgccgaa ctgatggtcg gaagaaagat ccaggccgtg acaggaaaag
     6121 acaagtgtga aaccgatatg gagtgccctg aaccgatcct ttcaatacga aacctgcatg
     6181 tcgagatgcc cggggaagaa cttcacggtg tcgacctcga cgttacacct ggggagatcc
     6241 tgggaatagg aggcctggct ggccatggga agatagggat cgccaacggg atcatggggc
     6301 tttacccatc caccggtaag atcgtcctaa acagtaagcc cgtacccctg aacaatccca
     6361 ttgaagcttt gaacagcgga atggccttcg tgtccgagga caggaggggt atcggccttc
     6421 tgctggaaga ttctattgag atgaacataa ttgtccaggc tatgcagagc cagggaaagt
     6481 ttttgaaaaa gggtatcatc cagcttatgg attttgcaac ctccaggcag cacactgaaa
     6541 aaatgataaa agaccttgat atccgctgca cgggacccga tcagattaca aggcggctca
     6601 gcgggggaaa ccagcagaag gtctgtatcg ctaaagccct gaccctcgaa ccccgggtgc
     6661 ttttcgtgtc ggaaccgacc agagggatcg atgtgggggc aaagcgcctg gtcctcaacc
     6721 tccttgtgga actgaacagg aaggccgggg tgaccattgt aatgacctcg tcggaactgg
     6781 cagagctcag gtcggtctgc gacaggatcg ccataatcta caagggacgt ctcgagggga
     6841 tactcccccc ggatgacagc aacgaaagat tcggcctcat gatggccgga gcgctgacac
     6901 accatatgaa ggaggtctcc tgatgccgga agacagcctc gttacagaaa aaaccacgtt
     6961 gaaggagagg gcccttgact tttaccggga ctttggcgta gcccgcctaa taatttttgc
     7021 gttccttgtg ttcctcctgg cttcagccct gttcctcaaa ctgcctatgg agcagatact
     7081 ttcgagcgtt cttacccgtt tcggcatgaa cggtatcctt gtccttgcta tgatcccatc
     7141 aatccagagc ggtgcaggac ccaattttgc ccttcctctc ggcataacct gcggtatagt
     7201 ggctgctacc cttagcatag aacttgacat ggctggtttt tcgggtctct tcttctcaat
     7261 agggttggca gccatcctgg gggctataac aggtttcttc tacggggtac ttctgaacaa
     7321 ggtcaagggg caggagatga ctgttggaac atattttggt ttttctattg tctccctgat
     7381 gtgcattttc tggttccttg ccccctacaa aagccctgag atgatctggc cttacggcgg
     7441 atcaggccta agggttacca tcgccattca ggacaggctg gggaaagtgc tggacaattt
     7501 cttgtccttc gacatcctgg gggtaacgat acccacaggc ctgctcctga tgttcggctt
     7561 attcagcctg gccctctgga ccttctccag aacaaagact gggatcgccc tttccacggt
     7621 tggatcgaac ccaagattcg ccgagtcgtc cgggcttaac gttaaccgct acaggataat
     7681 cggatcttca atatcccacg cccttggagc ggttggaata gtgatatacg cccagagctt
     7741 cgggttcatc cagctttaca tggcaccact tttcatggcc tttttcgccg ttgcgtccat
     7801 tcttatcgga ggagcaagcc tcaaaagggc caccgtcacc cacgccatca tcgggacatt
     7861 ccttttccag tccatactgg tcgtgtccat gccggtggca aacgtcgtcg tggcggacaa
     7921 catagccgac gtgataagga taatcataag caacgggatc atcctgtacg cccttacccg
     7981 cagggagatc gggggtgacg tgagttgacc gacgacaaca gggaccagtc caagaaactc
     8041 atgctccagg aaatcgaaga gactacttac aaggagcgca tctggaagta ctacctgagg
     8101 aagaacgtag tacctgtatt cttcctcgtc ctgtgtgtct ttggcggcta ctacagcggc
     8161 ctcagccttc cctttctcat caacgagata ataacccgta tggctcgaaa cagcttcctg
     8221 gtcatttcgc tgataatacc gatccttgca ggaatgggac tcaatttcgg gatagtcctg
     8281 ggggccatgg ccggacagtt cggatacatc gcgacggcca atttccagat ggcaggcttc
     8341 agcgggtttg cagtcacgac cctgatctcg atccctcttt cgatcctttt cggatggctc
     8401 acgggcaaac ttttcaacca ggccaaaggc aaagagatga tcacaggtat tatcctcggt
     8461 ttcttcgcca acggtatata ccagctgatc tgcctgtact ttctgggcaa cttcatacct
     8521 atcagggtac cgacgatgct tctttctacc gggatagggc taaggaacac tctggatatc
     8581 aagagcatgc attatgccct ggacaacctg tggaagatac gtatcaacat cccggggttc
     8641 atcttcccca ttatcatccc tgtcttcaca ctgctggcag ttgcgctcct gtgtctagcc
     8701 attcatttcc tgataaggac aaaacttggg caggagttcc gcgcggttgg acagaacagg
     8761 catatcgccg aggtcgccgg gataaaggtc gacaggacaa gggttatagc cattatcttt
     8821 tccacggttc tggccggaat aggccaggtc atcttccttc agaacctggg taacattaac
     8881 acttacggca gccatgtgca ggtcggcaca tttgcggtcg cggccatact gataggtggg
     8941 gcctccgtca gcaaggcgac catagggcag gcgctactgg gtacccttct tttccacacg
     9001 ctctttattg tctcacccct cgccggcaag aaccttatcg gcgacgcgat gatcggagaa
     9061 tatttcaggg tttttgttac ctacggggtt atcgggcttg ccctcgccct tcatgcctgg
     9121 caggctcgcc agaagcgcag atgaaggagg ccccttgaga tggctgcaaa ctccaggttt
     9181 tccaacgcct ttgatcttgt caggaaaaac ggttacgacg cctttgtaat ggcaccttcg
     9241 gcagaccttg agtaccttac aggcctttcg ccgcatcccg gggagagatt caacggtctt
     9301 ttcattttgg ccgatggcag atcttttgcc gtggtatcaa gactttacct ccaggaatat
     9361 cagcaaacac tccccggatc attgaccttg tacgactggg gagacgagga atggttcttt
     9421 ccagccctca agaaggcttt ctccgatcat gatattgctg aaggagccgt catcgccctc
     9481 aatgattcga ttatggccgt ggatgccgtc gagatcgcag aacgccaaaa ggtcagactg
     9541 gcaaacggat ggcacactct tgaaccgctc cgccttatca aagataaaga agaaatcgcc
     9601 tgcctgaaaa aggccggaga tatagccgac aaggccctta aatccctgtt gcaattcatc
     9661 cgacccggcg tttcagagaa aaccctccgc cgtaagcttc tggatcttct tgatgaccac
     9721 ggcgcaacat cccaggcttt cactccgata gtggcccgcg gcgagaacgc cgcaatgccc
     9781 cactaccccg gtggggatgg agttatcagg gaaaaagatt ctctgttaat cgattttggt
     9841 tgcagataca ggggatatta ttctgatatg accagaacaa tatttatcgg tgagccagac
     9901 catgaaacca gggaacttta cgatatcgtc ctcaggtcac aggatctggg agaaaaaacc
     9961 gcacgcccgg gaattacggc cgaagatctt gatatttcag caagatctgt cattgaggag
    10021 gctgggtacg gagatttttt caacaaccgt cttggacacg gcatcggaat agcgatccac
    10081 gaatcaccaa atatcatgag gggaaaccag atgacccttg caaagggtat ggctttcagc
    10141 atcgaaccgg gaatttacat accgggcagg atcggtatcc ggatcgaaaa ctgtgtcgta
    10201 ctgacagaaa acggatgcga acccctgacc ttgttcccca aagatctcct ggtagtctag
    10261 aaggaggatg gatatggaat atgaagccat gctcgaaaga tacagaccca gaataatatc
    10321 aaatctgcag gaaatgatcc gcataaggag cgtagaggcc cccgccgttg atgggaaacc
    10381 cttcggcgag ggtgttgacc aggctctccg cctggctctt gaaaccgctt ccatcctcgg
    10441 tctcagggca gtcaatcgga acggcatgat cggttatgcc gagtacgggt cgggcaagga
    10501 gatggtagcg gtccttgctc atctggatgt tgtccccgag ggaaaggggt ggactttccc
    10561 ccctttcggc gcagagatcc acgaccgaaa aatgtatgga cggggaacca ccgacgacaa
    10621 ggggcccgcc attgccgccc tctgggctct taaatttctg gcggaggaaa accatcgtct
    10681 ttcgaaaagg ataaggatca ttttcgggac taacgaggag tccgggtttc gcggcatcaa
    10741 gtggtatgcc gagaacgaag aaatccccgt tgcgggcttt acccccgaca ttcatgaggg
    10801 gatcgttttc gcggaaaagg gcggcctcca tgttcaaatg gatattcctg taaaccgtga
    10861 aggcagggtc tttctcgaag atttccgctc cggtgaggcg gtaaacatcg tccccgacct
    10921 tgcggaggct gttttctcag gagagatttc ggaactggat agttgggccg atgccctgga
    10981 aagccagaaa ggataccttc ctctcggggg agaggtaatc cgcgaaaaag agcgcctgat
    11041 aatcagggca aaaggcaagg cagcccatgc gagcctgccc gaaaacggaa taaacgcgat
    11101 cgcggcgtta tgctgttttc tgggcagatc ccctgaaccg gggatcttcg ataccctttc
    11161 gcaacttttc cgcgaccccg ccttcggaaa agaactggga atagaaatga cagacgaagt
    11221 ttcaggacca ctcacgtgca accccggact tgcctctttc tcggaaggca cccttagcat
    11281 caccctcgac ataaggcatc ctgtaacttt caccggtgat catgtcgagg aaaaaatccg
    11341 gggcatcctg ggacccctcg ggataaatat cagggttaca tcaagggcaa acccccttca
    11401 tgtcccgaag gattctcaac tgataaaaga cctctcggat gttttttttg aggttaccgg
    11461 caaacgtccg gagctcaagg caatgggagg aggtacctac gcaaaggccc tgcccaacgt
    11521 tgtagccttt gctcctttcc ccaacggtct cccggaactg gcacaccagg cagatgagta
    11581 catcgacatc gaagaatttc ttgcatccat acttgtaata aagaatgcct tgataaggct
    11641 tgcgagatag ttccacttta atgtggaaaa aatactacaa aaaagggggc ggacttatcc
    11701 gcccccgccc atttcatggt cgggtcttcc ggagttgtgc tcttccatcc agtcaaccac
    11761 tccctgttcc ttacagtgtc gcctctccgc caatcagcgg caacgccgtt tcctgtttcc
    11821 tgtttcctgt ttcctgtttc ctgtttcctg tttcctgttt ccttgctgtt tgaggcaggt
    11881 tccaaaactc tccggaaaac ccgactccgg ggaccacttc accccgatga aaagatacta
    11941 cactatttgg cggaaaatag caagggtttt ttctaaagtt gtcgcaaaaa acgttagtcg
    12001 tgaatcgtga gtcctgaatc ggaaaggcaa ggaagcagga gaccggagac aggaaaacct
    12061 aaaggcgtga acggtaaacg cctcacgccc ttgttttttc ccgtcccttt gatattgtta
    12121 tcttaaaaga tcttttcaat aatcgtttcc ccgggaggtc aagatatgga agaagccaga
    12181 ctgcttcgtc tcaagacaat gtccaacctt atcaggaagg acatcgtcaa aatggttctg
    12241 gatgccggct ccggacatcc aggcggttcc ctttcagtcg cagatatcgt atcagttctt
    12301 ttttttgatg ttatgactca cgacccgtcc aatccttgtt gggaaggcag ggaccgtttc
    12361 attctttcca agggacatgc gtgccccgcc gtatacgcag cattggcaga ggggggctat
    12421 ttcccaagag aagaactgaa cacccttcga aagttcggct cacgcctcca gggtcatccg
    12481 gatatgtgca aacttcccgg tctggaagcc tctactggaa gcctgggaca gggcctttcg
    12541 atagcttccg gacttgccct tgccatgaaa attgacggta aatccaacag ggtctactgc
    12601 cttatgggtg atggggaact cgatgaagga cagatctggg aagcagcaat gacagcatct
    12661 cacttcggcc tcgataatct ctgcgggata gtagatcgca acggccttca gatcgatgga
    12721 agaaccgatg atataaagac cctggaacct cttgccgaca agtggcgcgc ttttggctgg
    12781 aacgttattg agatcgatgg gcataatgtt acggaaatat ccggagcaat gaacaggttc
    12841 cgccataccc acttccgccc caccgttctt atcgcccata cgatcaaggg caaaggtgtc
    12901 tctttcatgg aaaaccaggc cggttggcac gggaaggccc cgagcaaaga gcaggcagag
    12961 gccgccctta tggaactgtc acaggaggtt gtttgaatga aagatcttca tcttgcaaaa
    13021 aacatgctgg atgaaccgga aaggatccct accaggttcg gttatggaga cggtcttgtt
    13081 gaactgggga agaccaatcc ttccgtcgtc gtccttggag ccgacctgag ttcttccctt
    13141 tgcgtcgatc gttttcgtga tgcctttccc gacaggttca tccagaccgg gatagcagag
    13201 caggatatga tgtcttttgc cgcagggctc tcactggctg gcaagatacc ctacgtcagc
    13261 acctatgggg ttttctgtac cggaagggca tgggaccagc tcaggaccac cgttgcatac
    13321 gccagactta acgtgaaggt gggtgcagga cacggcggcg tctctgtagg agcagacggg
    13381 gctacacacc aggccctgga ggatatttcc ataacccgca ccataccgaa cgttactgtt
    13441 atagttcccg ccgattacaa cgaaaccgta aaggctacga tacattccgc attaatacgg
    13501 gggccagtgg tgatccgttt cggacgagaa aaggtaccgg tagttacgac taaagaaaca
    13561 ccattcacgg tgggcagggc agagattttc catgaaggcg aggacgtatc cctgattgcc
    13621 tgcggacaga tggtttatga gtcaatggtc gctgctgaga ttctggccaa aaagggcgtt
    13681 tccgcaaggg taataaactg tcacaccgtc aaacccatgg acagggaaac catccttgcg
    13741 gcggcaagag aaacaggagc cattgtaacc gccgaggagc accaggtaat gggaggattc
    13801 gggagcgcca tagcagaggt ggtctgccag agctgccctg ttccgatcag gatgataggc
    13861 atccaggaca cattcggcca gtcaggagac cccgaggaac ttatgaaagc ctacgggcta
    13921 accggtcagg atattgcaaa aaccgcactt gatgccatag ccatgaagaa agggtgatag
    13981 gtgatgtcca tatcttttgc cgaaaggaca aagcgccttg ggaccgagac agccttcgct
    14041 gtaggagcag aggcggccca gcttgcctcg acgggagtaa aagtcttccc atttcacctg
    14101 ggggatctta atatacccac accagaacat atagcagagg catccagcaa ggctataaag
    14161 gatggaaaga cgggttactg ccccacccct ggaatattac ccctgaggga agctctggcc
    14221 gccgacattt cgaagtccag gaacctggat ctgaccgcag acaatgtcag catccagacg
    14281 ggcgggaaac cggtaatcgg caaatttatt atgtccctca tgaaccccgg tgacgaggtc
    14341 ctgtatccaa acccgggtta ccccatctac gaatccatga tcaacttcaa cgagggtatc
    14401 gggaaaccgt acggattcat tgaaacagca acaggctttg cccttgatat ggatgccata
    14461 gaaagacaga tcacccccag aacaaaacta cttatctaca acaattacca caacccaacc
    14521 agcgccgaat cagatgaaaa tgagatggaa aagctcgctg atatctgcgt caaaaacgac
    14581 ctctatgtcc ttagcgacga ggcatacttc gatatgcttt acgaaggcac cggcaaaagc
    14641 ataacaaggt tcccgggcct tttcgaaagg accgtcatcc tctacacctt ctccaaaaag
    14701 ttcgccatga ctgggtggag gctcggggcg gcaataggac caaaacccat aatcgacgca
    14761 atatccctct tcaacgttaa cgatgaatcc tgcaccaaca acttcgtcca gtgggccgga
    14821 ctcgcagcca taacgggatc catggaaccc tacaaaaaaa tgatcggaat actcagggaa
    14881 cggcgagatg cggccgtcga aatgctgaat aaaatgaacg gcgtttccgt ttcgactccc
    14941 gactccacct tttacctctt ccctaacgtg accggggcaa tgcggaaact gggattgacc
    15001 gatgttgagg aattccgcaa actggtgctt cacaagaccg gagtttcatt cactaccaga
    15061 gaccactttg gaactcctct tcctggtgaa acccagaaat acatccgcct ggcatactcg
    15121 ggcatcaaca ccccggagat catcgaaggg cttggaaaaa tgaaggagtt gctggggtaa
    15181 tcaagaacaa ggctacaggc agcaggcgtc aggcggcagg aaaaccttaa agcccgtgag
    15241 gcgtaaaccc gtgaatcgtg tagtctgtca ttctgagaac caacgggacg aagaatccgg
    15301 gttgcgttgt gttttgaact ttccgattca cgattcgcga ttcacgaaaa caaaccagat
    15361 ctccgccagc aggcgggaat ctgggtcaag gacagatccc cgccggtagg cggacgttcc
    15421 gcttcgctgc gctcaggatg acaacggggc aagggcgtca acggtcaacg gtgaacggtg
    15481 aacggtaagt tcaaaaaaca gaccaaaacc tcaaaccaac ttccgttgac acaaaaaagg
    15541 gccgggaagc tttattcagc tttccggccc ttttttattt tcgtggtttt tgtgtcccat
    15601 ggtcgttctg gttcatatat tgtgccttta cctggtttta ccgtttactg ttcaccgttc
    15661 acgcttttga tcttcctgtg gcctgttgcc tgccgccctg ggtttcaaat aaaaaaagaa
    15721 aagtttccgg cggcgtccta ctctcccacg gtttagaccg cagtaccatc ggcgctggag
    15781 ggcttaactg ccgggttcgg catgggaccg ggtgtgtccc ctccgccatg gccaccgaaa
    15841 acttttcctg taataccgtt aatcgttatt cgtgaatcgt taatcgtaaa tcgcaaagat
    15901 cttgcccttg ctcctggttt tacgtctcac gattcacgat tcacggggct tgccttttcc
    15961 gtttaccgtt taccgtttac cgtttaccgt ttacgctttt gatctcacgt ctcacgattc
    16021 acgcagatat
//
LOCUS       DAOTIQ010000043        15893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4441_length_15893_cov_6.792145, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000043 DAOTIQ010000000
VERSION     DAOTIQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 15893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15893
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4441_length_15893_cov_6.792145"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..1660)
                     /gene="trpB"
                     /locus_tag="P5541_06510"
     CDS             complement(461..1660)
                     /gene="trpB"
                     /locus_tag="P5541_06510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRV71671.1"
                     /translation="MKSMRKEVEARKGYFGTFGGSYVPEQLQPRLDELEAALSSAMGD
                     DLFAMEFTRLLKEYVGRPSALTYCENISRKYGGGSLFLKREDLNHTGAHKINNTIGQA
                     LLAKRMGKKEIIAETGAGMHGTATATVAALMGMKCTIYMGAVDVQRQAPNVLRMKALG
                     AEVVSVKHGQQTLKEAVDAALEAYVMNPDTFYLLGSAVGPHPFPTMVRGFQSVIGREA
                     REQFLAREGKLPDHVVACVGGGSNAIGIFSAFLDDPDVGIVGVEPAGRGLETGEHAAT
                     LMKGKPGIIHGFRTYVLTEDDGEPSPVYSISAGLDYPGVGPEHAHLMESGRASYVSVT
                     DDEAVEAFQVLCRSEGIIPALESSHAVAHALKMLPALSKEETVLVNLSGRGDKDLDSV
                     LPLLGLE"
     assembly_gap    1686..1785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1786..3310
                     /locus_tag="P5541_06515"
     CDS             <1786..3310
                     /locus_tag="P5541_06515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71672.1"
                     /translation="LLLVSSAVLLFLSGSTWIRRKNAGYPAVFLSLCMAGAALYNFGY
                     AMELSADNMDEVMTWVRFQHLGIEAIAPTWLLFSLHISGNGRVITSRRLVLLISVPVF
                     LFLSAQTLGDLNLYHISPRIDSSGAFSFFTYDRGLVAWISVTYMTFCVMASTVLFSIM
                     FHRTSPAFRKQAMIFLSGSIIPWLVMVLYMFGASPRGLDIVPFALTLSGAIFSFGLFR
                     YRILDIVPLARDAVFECMNDGVLVLDNLDRIIDFNSCLKDIVPEINPDSVGTCVFETL
                     AKYPDLLRCIGSDPSDELETDFKSPDSPACFKCRASNLVDWQGYQAGKIIMLSNFTQA
                     RQLMGQLEKLAALDGLTGIYNRRHFEDVAAREVYRFQRYGGSISLIMLDLDHFKNIND
                     AHGHAAGDAVLVSAAKTLASCLRKSDIMGRFGGEEFIVLLPETDRAAAIAIAEKMRQA
                     LESLMVRFQEQSFRVTASFGVTGVTSPESATPEIFFERADRALYEAKEKGRNRVCAA"
     regulatory      3386..3470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3733..4287
                     /locus_tag="P5541_06520"
     CDS             3733..4287
                     /locus_tag="P5541_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71673.1"
                     /translation="MQDKGKVKMAPVVSGEYVRKLRISLGLTQLQFARLMEVGNITIA
                     NWEKNGLDGTRSSSFPNFKYLTTLLKNSMRHPEMLEAEKLIRYLRLASEHELMAYYIP
                     YIDQMETDYLNVINSGSLTGVLFALLFDKELEKKGLSAPGDAGAGISGLKGFLQTEDE
                     PLLEEISREAAKTKTPGKKQGKGA"
     gene            complement(4304..4720)
                     /locus_tag="P5541_06525"
     CDS             complement(4304..4720)
                     /locus_tag="P5541_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV71674.1"
                     /translation="MKNIIQISEAASLALHGMGLLAIKGGRLSVREMALVTGSSEAHL
                     SKVFQRLSREGLVTSVRGPGGGFILARPAAEITLLDIYSAIEGEPKLEPCLLGKVKCP
                     FGKCIFGEMIPEITGRFIDHLSRTTLAEMANPSPGK"
     gene            complement(4826..6106)
                     /gene="glgC"
                     /locus_tag="P5541_06530"
     CDS             complement(4826..6106)
                     /gene="glgC"
                     /locus_tag="P5541_06530"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRV71675.1"
                     /translation="MIYGKHGRVLAVVLAGGKGERLMPLTKYRAKPAVPFAAKYRIID
                     FALSNLINSGLFSIYVLVQFKSQSLNEHIERGWQFGGAMRDRDHFVTLAPAQMWRGEH
                     WYQGTCDAVFQNLHLVTLYDADHICIFAADHIYKMDVEQMLDYHMEKKADVTVAANVV
                     PRSEASQFGCIQVDGSGKIIGFVEKPKDPPEIPGRPGYTYVSMGNYIFEREALEESVL
                     DDAQNENSSHDFGKDVLPVLHEKCSVYAYDFSTNVLPGRDRPYWKDVGTIKAYWEGHM
                     DLCRPNSDMTLYNPRWPIRTVSFSDPPAYSYPEGGKNCSEIGTLRAEGSRILGAVVHR
                     SVLSRQTLIRAGTIIEESIIGQGVEIGEGCRLRKVIVDAHNKIPPGTVIGHDLDEDRK
                     HYHVDEEAGVVVVGMPVMQLRKYLEIPVIPILER"
     gene            complement(6109..7608)
                     /locus_tag="P5541_06535"
     CDS             complement(6109..7608)
                     /locus_tag="P5541_06535"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRV71676.1"
                     /translation="MQTDTPLGSRISVLHVTPEMAPLSKIGGLADVAGSLPSALRENG
                     VDARVITPAWPGVMRRLAELGCPVDPGKPSVCVPLQWRAWCGSLSSTVVNGVPVYLLE
                     HPLLFENPSIYPAMLDKVSALPFAFLSLAALEPVFPDEWKPDIIHVHDWASCIIPAAL
                     KWHRYYSLLAKKPATLLTIHNLAHQGSLPIDVLEEWGMGPGAYGLDGLEYFGEANLLK
                     GGITSADLITTVSPSYAGEILTPAFGEGLEGVLKYRKEKIKGVLNGLDTISWDPLRDP
                     ALPARFGQGNMRGKKNCKNALLERCGWEASEGAVMVSVGRLVRQKGMDLVLECLDRIL
                     EGGGHLAVIGTGELVLEKAFSEAAATRPDRVFFLPGFDEGLARLAYAGGDIFLMPSLF
                     EPCGLSQMIAMRYGTVPVARKTGGLADTISDVDEQPGGTGFLFGESNPDALADAVARA
                     RARFSSPRQWSAVRKRCMEGDFSWKSSARAYVNLYRSLVLEGSAIKREG"
     gene            complement(7685..8254)
                     /locus_tag="P5541_06540"
     CDS             complement(7685..8254)
                     /locus_tag="P5541_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRV71677.1"
                     /translation="MSAQIMSRAWSSRTSQLIQGFLFGLVFGFLLQKGGVTKYDVIAG
                     QLLLRDFTVVKIMLTAVLVGMTGVYTMKWLGWVELYPKSGSLGMNVIGGLIFGAGFAI
                     LGYCPGTIAGAVGNGYMDALLGGVAGIMLGAGIFAALYPWLNEGVLKWGFYGKATLPE
                     IFKTGERKVVLIVAVVIIVILAVLEVARL"
     gene            complement(8271..8783)
                     /locus_tag="P5541_06545"
     CDS             complement(8271..8783)
                     /locus_tag="P5541_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRV71678.1"
                     /translation="MDYLKEIRWSPYVVGAGIGVLSWLSFLISRKPLACSTTFAKASG
                     MVEKMIRGEIVDSKPYYQKEKLVIDWQWMLVTGIVFGSLFSALLSSDFHFQLVPALWA
                     SRFGTGAVKRILASLLGGVFLGFGARWADGCTSGHGISGTMQLALSSWISAACFFIGG
                     IVTAKILFGF"
     gene            complement(8803..10254)
                     /locus_tag="P5541_06550"
     CDS             complement(8803..10254)
                     /locus_tag="P5541_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV71679.1"
                     /translation="MLLKHFFTGKIAHSSYILAGKNTCAVIDPQRDVDIYISEARSMG
                     VEITHILQTHLHADFISGHMDLAKKTGASIYVAKSAGCTFDHVPLSGGDRVEIEDMRL
                     DVLETPGHTPEHLAYVVTDISRSDSPVGAFVGDTLFVGDVGRPDLFPDMAEELAGKLY
                     HSLHEKLLKLPDYVEIYPAHGAGSLCGRAMGAKWTSTIGYERQFNPALQIGDKKDFVR
                     SLTENMPPAPDHFSRCSDINRHGPTLVSALPVMEELDPKSFLEKMKDPGTVLLDTRSY
                     FAFAGQHLEGAWHLDLNGNFPTFAGWVMPLDKDILLVADDFKSAQMANTWARRVGVDR
                     IVGYLDGGMANWTAQGLPSSHIEAISAGDLHEMIDGKSNFILLDVRAPLEFEDSHIEG
                     AVNIQVSDLRTRYVELEKEKPVVVMCSSGNRSSLGASILKQHGFRHVMNLAGGMSGYS
                     AAGFVKECRACMNPHGSRFFAKYSEVRKHWDIS"
     gene            complement(10316..>11584)
                     /locus_tag="P5541_06555"
     CDS             complement(10316..>11584)
                     /locus_tag="P5541_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRV71680.1"
                     /translation="FFSVTPVGVMLLIAGTGYFMIFGKYVLPGQGRGEVPLSVQDRII
                     EAFHLPHHIGHYRIPEESSLVGISTEKSGAWDVYNVNILGISEGMDTEYAPWRDSVFR
                     AGQELALLGSRDSVEAFAFDHGLRSLEEPRRFAGLDDPGRAGFAEVIIPPRSEMVAKT
                     IRQFSLRKRFAVEPVMLFSKGEEIRGDFSDHKIDPGDTLIVHGLWENITALREEAGFV
                     VATPFVAERRDRSRIGVALACFLGSIALVLAGAPISMAFFSGAIAMVLAGVISIQEAY
                     NAIDWKVVFLLGGLIPLGTAMQKTGTAALLAEKLMTLVQGGHPVLLVLTVALLSTVFS
                     LLMSNVGAVMVLAPLVMNMGVIGGVDPRPLALLAAVCVANSFLLPTHQVNAFLMSPGG
                     YTNADYIKAGGGMTLVFIVVAVTAFLVLFI"
     assembly_gap    11585..11684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11685..11887)
                     /locus_tag="P5541_06560"
     CDS             complement(<11685..11887)
                     /locus_tag="P5541_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71681.1"
                     /translation="MMDSSMLVVLTILMVTVFFMIFDVFRIDIVAILCMLALGWTGIL
                     TPGEVFSGFSSNAVIAMMAVMILG"
     gene            12179..12964
                     /locus_tag="P5541_06565"
     CDS             12179..12964
                     /locus_tag="P5541_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRV71682.1"
                     /translation="MNTLLLVLLMVFLYMSLFFLIAVVRKDNSLADIAWGGGFVLIAF
                     VTFLLAPGYAEARQVLATGLVTLWGLRLAIYIHFRNLGRGEDYRYAKWRKDWGKNFLI
                     RSFLQVFMLQGALMILIASSVIFINLNPARGLTFLDALGLAIWCVGFFFESVGDFQLF
                     QFKKDPGNKGRVMRYGLWKYTRHPNYFGEATMWWGIFLMALSLKGGLVAVLSPLAITF
                     LLLKVSGIPMLEKKMAETQEGYNEYMKSTSSFFPWFPGKNAGR"
     gene            13040..13912
                     /locus_tag="P5541_06570"
     CDS             13040..13912
                     /locus_tag="P5541_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71683.1"
                     /translation="MKNRVCLLIMTALLLWTLPAAADHSASGTVSGPTPYVYTIKCDP
                     GERFLVEVTSTQPTSVNILSMTPNSRADGGWAFNAVKSSQQAYSHLLDHSAPSGEPRN
                     NASHWHYRVSILASTYEPADFTLSVSLFKDGSDDFNKRAREQLVDLGKNLDQENKSLK
                     EKIAAMSWLKDTVEELNERKARLMENYEEIERIIALVKSETDPQEKQRLVDIGKTLVA
                     EGKADEAQYKDDYQKIKADLELYNSMIRKRKAVQDLGNSLEDPFNRKDYAACVAIANG
                     SDIARELGWVILEK"
     gene            13933..14496
                     /locus_tag="P5541_06575"
     CDS             13933..14496
                     /locus_tag="P5541_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71684.1"
                     /translation="MKIPLYGVLLLFVPVLLFCAAFPVLAGEAVSPCDLFTRPDAEGL
                     FGCPVSEGVFRVATSPAGGSCRYSYEKDGNVYGITVRVADTVSITEEGIYDSAKDVFD
                     RQVRARKASEEASKKYKEIENLGDGAFWEGTSLWMLLEDSLVIIKVNSFLPGSFSGQE
                     QMDSAREEQDLSFSLKAAEIILQRLNK"
     gene            14517..15584
                     /locus_tag="P5541_06580"
     CDS             14517..15584
                     /locus_tag="P5541_06580"
                     /inference="COORDINATES: protein motif:HMM:NF027559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HRV71685.1"
                     /translation="MARNVAFAIIASLLLAVSPVTAATEVPLADIPGSQDCDLLGRFE
                     GSVIVSFEKKDFDEFTAPLSPLEPVKDKRDPHNNILFEPAEKMTLEGEHWRIVYLIPE
                     GASPLEVIRNYQQEILDAGGETLFECKGSECGGDPNRGSSGGGGDMSLAMYLRPDKSI
                     KDIEFSPGSCAQRERITDQRYLAATLPEGELVSVLTYSINPGNACKLFRDRTVAAVDI
                     VIPKKREQKMVTVKAEEMASQISSTGSIALYGIFFDTNKWDVKQESEPTLEEIAKFLK
                     GNPSISILVVGHTDNVGTFDFNLELSRKRAGSVVSALAEKFGIDRKRLFPVGVSFASP
                     VASNSTEEGRSKNRRVELVEN"
BASE COUNT         3687 a         4421 c         4012 g         3573 t
ORIGIN      
        1 ttcccgcaaa accggcgata aacttgaaaa gggtgagaag aacattcacc ccaagcccga
       61 cccatgagac aaaaagtgct ttttcggttc tctggttatt caatcggaaa atccccccat
      121 cggggcaatg tgtcgtttcc gacaatttta ccccaacggg gggatacttt tgtccttcca
      181 gctgtttcct tgtcttgcct ggctttttgt tctttttgtc attctgagga tcaacgggaa
      241 tgacaaaacc aaaggcgtaa acggtcaacg gtcaacggtg aacggtgaac ggaaagggca
      301 gatctccgcc ggtaggcgga cgttccgctt cgatcgctac gctcctcagg atgacagtcc
      361 tttcttgtca ttctgaaccc gaagggtgaa gaatctggtt tttcgattca cgattcccga
      421 ttcacggttc acggggttta ccagttacga gttacggaat ttattccaat cccagcagcg
      481 ggagtaccga atccagatcc ttgtcccctc tcccggaaag gttcaccaga accgtctctt
      541 cctttgaaag agccggaagc attttcaggg cgtgcgccac ggcatgggaa ctctcgagag
      601 ccggaatgat cccctcgcta cggcagagaa cctgaaaggc ctcaacagcc tcatcatcag
      661 taacagagac gtaggatgcc cggccgcttt ccatgagatg cgcgtgctcg ggaccaaccc
      721 cgggatagtc aagacccgcc gagatggagt agacggggga tggttcgcca tcatcttcgg
      781 tcagcacgta ggttctgaat ccatgaataa tccccggttt ccctttcatc agggtcgcgg
      841 cgtgctcccc tgtctccaga ccccttccgg cgggttccac accaactatc cccacgtccg
      901 gatcgtcaag gaatgccgaa aaaataccta tggcgttgct tccaccaccg acacaggcaa
      961 ccacatggtc gggcagtttc ccctctcttg caaggaactg ctcccgtgcc tccctgccta
     1021 taacgctctg gaatcccctg accatggtcg ggaaggggtg gggcccaacg gcagagccca
     1081 gcaggtagaa ggtatccggg ttcattacat aggcctcgag tgccgcgtcg acggcctcct
     1141 tgagggtctg ctgtccatgt ttcacggaca cgacctcggc cccaagagcc ttcatgcgca
     1201 aaacgttggg agcctgtctc tgcacatcga cggctcccat gtagatggtg cacttcattc
     1261 ccataagggc agccaccgtg gctgtcgcag taccatgcat tcctgccccg gtctctgcga
     1321 tgatctcctt tttgcccatc cgtttcgcaa ggagcgcctg cccgatggtg ttgttgatct
     1381 tgtgggcgcc agtatgattg agatcctcgc gcttgaggaa aagactgcct ccaccatatt
     1441 tgcgggatat gttttcgcag tatgtcaaag ccgacggcct tcccacgtat tccttcagaa
     1501 gcctggtgaa ttccatcgca aaaagatcgt cacccattgc agatgataaa gccgcctcca
     1561 gttcgtcaag cctcggctgc aattgctccg gcacatagct cccgccgaaa gtcccgaaat
     1621 atccctttct tgcctcaact tccttcctca tactcttcat tccctcttcc tcctctgctc
     1681 atcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttac tgctcgtatc
     1801 ttcggcagtt ctgctgtttc tttccggaag cacatggatc aggaggaaaa acgccggtta
     1861 ccccgctgtt ttcctctctc tctgcatggc tggggccgct ttatacaatt tcggctacgc
     1921 catggaacta tcggcggata atatggatga agttatgacc tgggtcaggt tccagcacct
     1981 cggtatagag gcgatcgcac cgacatggct cctgttctct ttgcatatat caggaaatgg
     2041 aagggttata acctcgcgac gtctcgtgct tcttatctcg gtgccggtat ttctcttttt
     2101 gtccgcccag accctggggg acctgaatct ttaccacatc tctccccgca tcgactcttc
     2161 gggggctttc tctttcttta cctacgacag gggacttgtt gcctggataa gcgttacgta
     2221 tatgactttc tgcgtcatgg ccagcactgt ccttttttcg ataatgttcc acagaacatc
     2281 tcctgccttt cgcaaacagg caatgatatt tctctctggg tcaatcatcc cgtggctggt
     2341 catggtcctt tatatgttcg gggcttctcc cagaggcctc gacatcgttc ccttcgctct
     2401 gaccttgagc ggggctatct tttcttttgg gctgttcagg taccggatcc tggatattgt
     2461 ccccctggca agggatgctg tattcgagtg catgaacgat ggagtcctgg tcctcgacaa
     2521 tcttgacaga ataatcgatt tcaattcctg cctgaaagat atagtgccag agatcaatcc
     2581 ggattccgtc ggaacctgtg tctttgaaac tttggctaaa taccctgatt tgctccgatg
     2641 cataggaagc gacccgtcgg atgaactgga aactgacttc aagagccccg atagccctgc
     2701 ctgtttcaag tgccgggcat cgaatcttgt cgactggcag ggatatcagg cgggaaagat
     2761 cataatgctg agcaatttta cacaggctcg ccaactgatg gggcaattgg aaaaactggc
     2821 ggctctcgac ggccttaccg gcatttataa ccgaagacac ttcgaagatg tggcggcgag
     2881 ggaagtttac cgcttccaga gatacggcgg atcgatctcg ctgattatgt tggatctcga
     2941 ccatttcaag aacatcaacg acgctcacgg acatgccgca ggcgatgccg tcctggtctc
     3001 ggcagcgaag accctggctt cctgcctgcg gaagtcggac attatgggac gtttcggggg
     3061 agaggaattc atcgtacttc ttcccgagac cgatcgggcc gccgcaatcg cgatagccga
     3121 gaaaatgaga caggcccttg aatctctcat ggtacgtttt caggaacagt cgttcagggt
     3181 tacagccagt ttcggcgtaa cgggagtcac atccccggaa agcgccaccc ccgagatttt
     3241 tttcgaacgt gctgaccggg ctctttacga agccaaggaa aagggacgca acagggtctg
     3301 cgctgcgtga acccttgccc ctgaagcaat tacatcaacg ttcgagtctt gcaatattgc
     3361 atttgtctat tataatgtaa gtaagaaccg aaaaaggcaa aatcgtcgaa aggcgatgac
     3421 gcaaaaccgc gggtctaacg cttattagcc atgacagccg ggttgccgag tttcagaggg
     3481 tttttccaga agcttccggc cccttacgga ggcttatttt tttgggaggt tcattctcat
     3541 gcgcccggtc acggcaatcc tgactcatgg cattatcaga tcatcctcga caaaaccgca
     3601 cagcgagtca aaggcgtctc cttcctgtga cagggaccat ccctctcgat tgattcaact
     3661 ccttaacaaa tatttgcggg gtgtctatcc tgcccacccg ctgcgaaccc actttgaagg
     3721 gagaagcaaa caatgcagga caaaggtaag gtaaagatgg ccccggtggt atcgggggaa
     3781 tacgtaagga agctcaggat aagcctgggt cttacacaac tgcagttcgc ccgactcatg
     3841 gaggtcggca acatcaccat tgccaactgg gaaaaaaacg gcctggacgg aaccaggagt
     3901 tccagtttcc cgaacttcaa gtacctgacc acgctgttga aaaactccat gaggcacccg
     3961 gaaatgctgg aagccgagaa acttataaga tatctccggc ttgcgtcgga acatgaactg
     4021 atggcgtact acatacccta tattgatcaa atggagacgg attatttaaa cgtgatcaat
     4081 tccggaagtc ttacaggggt cctcttcgcc ttgctcttcg acaaggagct cgagaaaaaa
     4141 ggtttgtcgg cccccggtga cgccggggcg ggcatctcgg gactaaaggg ttttctccag
     4201 accgaggatg aaccattgct cgaggagata agccgcgaag ccgcaaagac aaagacaccc
     4261 ggtaaaaaac aggggaaagg cgcctgatcg cctttcccct gttttattta ccgggggagg
     4321 ggttggccat ttccgcgaga gtggttcggg agaggtgatc tataaaacgg cctgttattt
     4381 ccgggatcat ctccccgaaa atacatttcc cgaaagggca ttttactttg cccagcagac
     4441 agggctccag cttcggctcc ccctctatgg cagaataaat atccaggagt gttatttctg
     4501 ccgccggacg ggccagaata aaaccaccac cgggtccacg tactgatgtc actaaacctt
     4561 cccgggacaa cctctgaaaa acctttgaaa gatgcgcctc cgaagaaccg gtcacgaggg
     4621 ccatttcgcg gacgctcaag cgccccccct tgatggccag aaggcccatt ccgtggagcg
     4681 ccagcgaggc agcctcggat atctggatta tgttcttcaa ttaacacctc gaaatcacaa
     4741 atatgcccct ctggagggac tggctggaga taaaaacaat aaccgataga agtaccttta
     4801 tactaaatta tacaacatgg tggaactagc gctccaggat cggaataacc ggtatttcga
     4861 ggtattttct caactgcatt actggcatgc caaccactac tacgcccgct tcctcgtcca
     4921 cgtggtagtg tttccggtcc tcgtcaagat cgtgacctat taccgttccc ggcgggatct
     4981 tgttatgggc gtccacgatg accttcctca gcctgcaccc ttcccctatc tcgacgccct
     5041 ggccgatgat gctctcttca atgattgtcc cggccctgat aagggtctgt ctcgaaagga
     5101 cagacctgtg gactacggca ccgaggatcc tgctcccctc tgctctcagg gtccctatct
     5161 ccgagcagtt ctttcccccc tcgggatatg agtaggcggg tgggtcggag aaggaaaccg
     5221 tccggatcgg ccacctcggg ttgtagaggg tcatgtccga gttgggtctg caaagatcca
     5281 tgtgcccctc ccagtaagcc tttattgtcc cgacatcctt ccagtacggc ctgtcccttc
     5341 ccggaaggac atttgtcgag aaatcgtagg catagacaga gcatttctcg tgaagaaccg
     5401 ggagaacatc ctttccgaaa tcgtggctgc tgttttcgtt ttgagcatcg tcaagaaccg
     5461 attcttcgag ggcttccctt tcgaaaatat aattccccat cgagacgtag gtgtagcccg
     5521 gccttccggg aatctccgga ggatctttcg gcttttcgac aaaaccgatt attttcccag
     5581 agccgtcaac ctgaatgcac ccgaactgcg aggcctcgga cctgggaacc acgttggcgg
     5641 cgacggtcac gtcggccttc ttctccatgt ggtagtcgag catctgctcg acatccatct
     5701 tgtagatgtg gtctgccgca aatatgcata tatggtcggc atcatagaga gtaacgaggt
     5761 ggaggttctg aaagaccgcg tcgcatgtcc cctggtacca atgctctccg cgccacatct
     5821 gggcgggagc aagggtcacg aaatggtccc ggtcacgcat ggctcccccg aactgccacc
     5881 ccctctcgat atgttcattc agggactggc tcttgaactg gacgaggacg taaatcgaga
     5941 agagcccgct gttgatgagg ttggagaggg caaagtcaat gatcctgtat tttgcggcga
     6001 agggaactgc aggcttggcc ctgtatttgg tgagtggcat cagccgctcc cccttgcctc
     6061 ccgcaagcac caccgcaagg actctcccgt gtttgccata gatcatgtct atccctcccg
     6121 tttaattgca gatccttcga gaacaagaga tcgataaagg tttacgtagg cacgggcaga
     6181 acttttccac gaaaaatccc cctccatgca acgctttcgg accgccgacc actgccgggg
     6241 agaggagaac ctggcccttg cccttgctac cgcatcagcc agagcatcgg ggttgctctc
     6301 tccgaagagg aaccctgtcc ccccgggctg ttcgtcaaca tcggatatcg tgtctgcaag
     6361 gccccctgtt tttctcgcca ccggaacggt cccgtaacgc atggcaatca tctgggagag
     6421 accacagggc tcgaacaagg aaggcatgag aaagatgtct cccccggcgt aggcaagtct
     6481 ggccagtccc tcatcgaaac caggcaggaa gaagaccctg tcaggtctcg tcgcggctgc
     6541 ttccgaaaaa gctttctcca ggaccagttc ccctgtgccg atcaccgcga ggtggccccc
     6601 tccctcaagg atcctatcga ggcactcaag gaccaggtcc atccccttct gcctgacaag
     6661 ccttcccacc gagaccatta cagctccttc ggaggcttcc catccgcatc tttccaatag
     6721 agcattcttg cagttttttt tgcccctcat gttaccctgg ccaaatcggg ctggcagagc
     6781 cgggtccctc aagggatccc aggaaatagt atcgagaccg ttaagaacac cctttatctt
     6841 ttcctttcga tatttcagca ctccctcaag accctctcca aaggctgggg tcaatatttc
     6901 cccggcatag gagggactca cggtagtaat aagatcagcc gatgttatcc cccctttgag
     6961 taggttggcc tccccgaaat actccaggcc atccaggcca taagccccgg gacccattcc
     7021 ccactcttcc agaacatcaa tgggcaggct cccctggtgg gccaggttgt ggatcgtaag
     7081 gagagttgcc ggtttttttg cgagaaggga gtagtacctg tgccatttca aagccgcggg
     7141 gataatgcag gatgcccagt catgaacatg gattatgtca ggcttccatt cgtcaggaaa
     7201 caccggttcc agcgccgcca gcgagaggaa ggcaaaggga agagccgaga ctttgtccag
     7261 cattgcagga tatatggaag ggttctcgaa aagcaggggg tgctcgagaa ggtaaacggg
     7321 gaccccgttt actacggtgc tactcaggga tccgcaccag gccctccact gcaagggcac
     7381 acatacagaa ggcttcccgg ggtcaacggg gcacccaagt tctgcaagtc gcctcattac
     7441 cccaggccag gccggcgtaa tgacccttgc gtccacgccg ttttccctca aggcagaggg
     7501 cagcgacccc gcgacatcag caaggccccc gatcttggat agaggcgcca tttccggggt
     7561 tacatggagt acggagatac gggagcccag tggagtatcc gtctgcatcg cctcccggct
     7621 acaggaccct ttgtataaat gctattttac tatcattaaa aagttttgcc aatttacaga
     7681 aaatttacag ccgtgccact tccaaaactg ccagaatcac gatgatcacc acagccacta
     7741 tcagaaccac cttgcgttcc ccggtcttga agatctccgg gagggtggcc tttccgtaga
     7801 aaccccactt gaggaccccc tcgttcagcc aggggtaaag ggccgcaaag atcccggcac
     7861 ccaacatgat ccccgcgact ccaccgagaa gggcatccat gtagccgttc cctacggccc
     7921 ccgcgatcgt tcccggacag tagcccagaa tggcaaaccc ggccccgaag atcaggccgc
     7981 cgatcacgtt catccccaga gaaccggatt tcggataaag ttccacccag cccagccatt
     8041 tcatcgtgta gacccctgtc atacctacaa gcaccgcggt aagcattatt tttactaccg
     8101 taaaatctct cagcagaagt tgccccgcta taacatcgta ttttgtgaca ccgcccttct
     8161 gaagcagaaa accaaaaacc aggccgaaca gaaaaccctg tatcagctgg cttgtccgac
     8221 tgctccaggc acgtgacatg atctgtgctg acatctgctc ataccccccg tcaaaacccg
     8281 aagaggatct ttgccgtaac gatccccccg atgaagaagc aggcagccga gatccagctg
     8341 gacagggcca gctgcattgt cccgcttata ccgtgaccgc tggtgcagcc atcggcccac
     8401 cttgccccga aaccgaggaa caccccaccc agcagggaag ccaggatcct cttcaccgct
     8461 cctgtaccga accttgaggc ccatagggcg ggcaccaatt ggaagtgaaa atcactggac
     8521 agaagagccg aaaagaggga cccgaaaact atccccgtaa caagcatcca ctgccagtcg
     8581 atgacaagtt tctctttctg gtaatagggt ttgctgtcga cgatctctcc gcggatcatc
     8641 ttctcgacca tcccgctcgc ttttgcgaaa gtcgtggaac aggcaagagg ctttcgggat
     8701 atcagaaaac tgagccagct cagcacaccg ataccggctc ccacaacgta gggcgaccaa
     8761 cggatctcct tcaggtaatc cacttcctct tccccccttc tctcaggata tgtcccagtg
     8821 tttccttact tccgaatatt ttgcaaaaaa cctggaaccg tgggggttca tacaggcccg
     8881 gcattccttg acgaacccgg cagcgctgta gccggacatg cctccggcaa ggttcatgac
     8941 atgcctgaac ccatgttgct tgagaatact cgccccgagg ctcgaacggt tgccggaact
     9001 gcacatgacc acgaccggtt tttccttttc caactccaca tatcgggtgc ggaggtcaga
     9061 gacctggatg tttacagcgc cctcaatgtg gctgtcctcg aactcgaggg gcgccctgac
     9121 atccagaaga atgaagttgg attttccgtc gatcatttca tggagatccc cggcggagat
     9181 cgcctcaata tggcttgacg ggagcccctg ggcggtccag ttcgccatgc cgccgtcgag
     9241 atacccgacg atcctgtcca cgccaaccct tcgggcccag gtgttggcca tctgggcact
     9301 tttgaaatca tcggctacca gcaggatatc cttgtccagg ggcattaccc aaccggcaaa
     9361 ggtggggaag ttgccgttga gatccaggtg ccatgccccc tcgaggtgct gacccgcaaa
     9421 ggcaaaatag ctcctcgtat caagaagaac cgtgcccggg tctttcatct tctcgaggaa
     9481 actcttcgga tccagttcct ccatgacggg aagggctgat accagggttg ggccgtggcg
     9541 gtttatatcg ctgcaccggc tgaaatggtc cggagccggg ggcatgttct ctgtcagtga
     9601 ccgtacgaaa tcttttttgt ctcctatctg cagggcaggg ttgaactggc gctcgtaacc
     9661 tatggtgctg gtccactttg cccccattgc ccttccgcag agagagcccg caccatgggc
     9721 aggatatatc tccacgtaat caggcagttt aaggagtttc tcgtggagac tgtgataaag
     9781 ttttcccgcc agttcctccg ccatgtcggg gaaaagatcc ggccttccga catcacccac
     9841 gaaaagagtg tcccccacaa aggccccaac gggactgtct gacctggaaa tatcggtcac
     9901 gacataggcg aggtgctccg gagtatgacc cggggtctcc agaacatcga ggcgcatgtc
     9961 ctctatctcc actctgtctc ccccggacag tggaacgtga tcgaaagtgc acccggccga
    10021 ctttgccaca tagatcgaag ctccggtttt ctttgccagg tccatgtgac cggagataaa
    10081 atcggcgtgg agatgagtct gaagaatatg tgttatttcc acccccatcg aacgggcttc
    10141 gctgatgtag atgtccacat ccctctgggg atcgataacg gcacaggtgt tcttgcctgc
    10201 cagaatgtag gaactgtggg cgatcttccc tgtgaagaag tgtttcagaa gcatgatcct
    10261 tcctcctctc cgatttataa gtattgacct tgaaagactc tccgtgactt caatttcaaa
    10321 tgaaaagaac cagaaatgca gtaacggcga caacgataaa aaccagcgtc atcccccctc
    10381 cggccttaat ataatcagca ttggtgtacc cacccggcga cataaggaag gcgttgacct
    10441 ggtgggtagg caaaagaaac gagtttgcca cgcagacggc agcaagaagc gcaagggggc
    10501 gggggtcgac accaccgatc actcccatgt tcatgaccaa tggtgccaga accatgacag
    10561 cccccacgtt ggacatcagc agggagaaaa cagttgaaag cagcgcaacg gtaaggacca
    10621 ataaaacggg gtgacccccc tgtacaaggg tcatcagttt ctccgccagc agtgcggccg
    10681 tgcctgtttt ctgcattgcc gtgcccaggg ggataagccc ccccaaaagg aaaacgacct
    10741 tccaatcaat ggcattgtag gcttcctgga tcgatattac gccagccagc accatggcaa
    10801 tggctccgga gaaaaaggcc atcgatatcg gggcccctgc cagcaccagg gctattgatc
    10861 ccagaaaaca ggccagggcc accccgatcc tgctccggtc ccttctctct gctacgaagg
    10921 gcgtggcgac aacaaagccc gcctcctccc tgagtgccgt gatattttcc caaaggccgt
    10981 gcactatgag tgtgtcgccc ggatcgatct tgtgatcaga aaagtcaccc ctgatctcct
    11041 cacccttgct aaaaagcatt acgggctcga cggcaaagcg ttttcgcagg gagaactgcc
    11101 ggatcgtctt tgcgaccatc tcggacctgg gaggtattat cacctcggca aatcccgccc
    11161 tcccgggatc gtcaagcccc gcaaaccttc ggggttcttc aagagatctt aatccgtggt
    11221 cgaacgcaaa ggcttccaca ctatcccgac ttcccagaag ggccagttcc tgccccgcac
    11281 ggaaaacgga atctcgccag ggggcatatt cggtatccat tccttcggat attcccagaa
    11341 tattgacgtt gtaaacgtcc caggctcccg acttctccgt ggagatacca acaagggaac
    11401 tttcttcggg aattctataa tgcccgatat gatgaggaag atgaaaagcc tcgatgatcc
    11461 tgtcctgcac tgataatggg acctctcccc gcccctggcc tggaagaaca tacttcccga
    11521 agatcatgaa atatccggtg cccgcgatca gaagcatcac cccgacaggg gtaacgctga
    11581 agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccagt atcatgacgg
    11701 ccatcattgc tatcaccgca ttgcttgaaa aaccgctgaa gacctccccg ggggtcagga
    11761 tccccgtcca tccgagggca agcatgcaca agatagccac aatgtcgatc ctgaacacat
    11821 cgaaaatcat gaaaaacacc gtaaccataa gtatcgtcag aacaacaagc atggaactgt
    11881 ccatcatgtc ccgcccccct tttaatatgg gccggtcctt tcctgacatc ctttcagata
    11941 cattgtaata tctctctgaa gcgttgtctc gctctgctct ttgttcggtt tttccagaaa
    12001 ggctcgaact ccatctccga tgttgagaaa acccgggcgc ccttcccaaa accctggaat
    12061 gataatatca tctatatgaa tctagaaact gctatccgtt tgtcgcaata acatgggatg
    12121 gcatcctgag accctttatc agtccgggat cagcccggac aatctggagg tggcctcgat
    12181 gaatactctt cttcttgtat tgctgatggt ttttctttac atgtctcttt tcttccttat
    12241 agcggtagtg aggaaggata attcccttgc ggatattgcc tggggaggcg gctttgtcct
    12301 gatagccttt gtcactttcc tgctggcccc cggatacgcg gaggccagac aggtactggc
    12361 gactggcctt gtgacactct ggggcttgag gcttgctatc tatatccact tccggaacct
    12421 tggcagggga gaggactacc gctacgcaaa gtggcgaaaa gactggggca agaatttcct
    12481 tatcaggagt ttccttcagg ttttcatgct ccagggagca ctgatgatcc tgatcgcctc
    12541 atcggtgatc ttcatcaacc ttaacccagc cagggggctt acctttctcg acgctctggg
    12601 gctggcgata tggtgcgtag gttttttctt tgaatccgta ggggatttcc agctcttcca
    12661 gttcaagaag gaccccggaa acaaggggag ggtgatgcgt tacgggctgt ggaagtacac
    12721 ccggcacccc aactattttg gcgaagcgac catgtggtgg ggtatcttcc tgatggctct
    12781 ctctctcaag ggaggcctcg tcgcggtatt aagccctctg gcgataacct tcctgcttct
    12841 gaaggtatcg ggcataccga tgctggagaa gaagatggcc gagacccagg aaggttataa
    12901 tgagtacatg aaaagcacca gctcgttctt cccctggttc ccgggaaaaa atgccggcag
    12961 atgaaaccgg ctgttctttc ggggccagtg gccgtgaatt ggacccgcga gaaaacaccg
    13021 atgacaagga gatgggaaag tgaaaaatcg ggtatgcctg cttatcatga ctgcactttt
    13081 attgtggact ctgcccgcgg cggcggatca ttcggcatcg ggaacggttt ccggacccac
    13141 cccctatgtt tacaccatca agtgcgaccc cggtgaacgc ttcctggttg aagtcacttc
    13201 cacccagcca acctcggtga acatcctttc aatgacgccc aattctaggg cagacggagg
    13261 ttgggccttc aacgccgtga agagcagcca gcaagcctac tctcatcttc tcgatcacag
    13321 cgccccctcg ggagaaccca ggaacaacgc ctcccattgg cattacaggg tttcgatcct
    13381 ggcctcgaca tacgaacccg cggattttac cctttctgtt tccctgttca aagacggttc
    13441 ggatgatttc aacaaacgcg ccagggaaca gttagtagat ctcggcaaga acctggatca
    13501 ggagaataaa tcactcaaag aaaagatagc agccatgagc tggttgaaag acacggtgga
    13561 ggaactgaac gaaagaaaag caaggcttat ggagaactat gaggagatag aaagaatcat
    13621 cgctcttgtg aaaagcgaga ccgatcccca ggaaaagcaa aggcttgtcg atatcgggaa
    13681 aactcttgtg gcagaaggca aggctgacga agcccaatac aaagatgatt accaaaaaat
    13741 aaaggctgac ctggaacttt acaattcgat gatcaggaag cgaaaggctg ttcaggacct
    13801 ggggaactct ctcgaagacc ccttcaacag gaaagattac gccgcctgtg tcgccatagc
    13861 caacggatcg gacatcgcca gggaactcgg ttgggttatt ctggaaaaat aatcgaaaaa
    13921 ggggtggcgt ccatgaaaat cccgctgtac ggtgtcctgc tgctatttgt tccggtcctt
    13981 cttttttgtg ccgctttccc ggttctggcc ggagaagcgg tttccccctg tgatcttttt
    14041 acaagacctg atgccgaagg tctgtttggc tgccctgttt ccgagggcgt ttttcgtgtt
    14101 gcgacctccc ctgcgggagg atcgtgcaga tactcttacg aaaaggatgg caacgtttac
    14161 ggcatcactg tccgggtggc cgataccgtt tcaataacgg aggagggcat ctacgactcc
    14221 gcaaaagatg tcttcgacag gcaggttcgt gcccgcaagg caagtgaaga ggcttcgaaa
    14281 aaatataagg agatcgaaaa cctcggggac ggggctttct gggagggaac atctctctgg
    14341 atgcttctgg aggattcgct ggtaatcata aaggtcaatt cttttctccc cggttccttc
    14401 tctggccagg aacagatgga ttcggcccgg gaagagcagg atctgtcctt ttccctgaaa
    14461 gctgcggaga tcatcctcca gcgcctgaac aaatgagaaa gaaaggggac ctgaagatgg
    14521 caaggaacgt tgcgttcgcg attatagcgt cgctgctgct ggcagtctct cctgtgacgg
    14581 ccgccacaga ggtgcctttg gccgatattc cgggatcaca ggattgcgac ctgctcggcc
    14641 gtttcgaggg atccgttatt gtttccttcg agaaaaagga tttcgacgaa ttcacggctc
    14701 ccctctcccc gctggaaccc gtcaaggaca aaagggatcc ccataacaat atcctgttcg
    14761 aacccgcaga aaaaatgacc ctcgaagggg agcactggcg aatagtctat ctaatcccgg
    14821 aaggtgcctc ccccctggaa gtcatcagga actaccagca ggagatcctt gacgcggggg
    14881 gcgagaccct tttcgaatgc aagggcagtg aatgcggagg tgaccccaac agggggagca
    14941 gcgggggagg cggtgacatg agcctcgcca tgtacctccg tcccgacaaa agcatcaaag
    15001 acatcgaatt ctcacccggt tcctgtgccc agagggaaag gatcaccgac cagagatacc
    15061 tggcagcgac tcttcccgaa ggggaacttg tctcggtcct tacatacagt atcaacccgg
    15121 gaaacgcctg caagcttttt cgggatcgga cggtagcggc cgtggatata gtcatcccaa
    15181 agaagcggga acagaaaatg gtgacggtga aggccgagga gatggcatca cagatatcca
    15241 gcaccggcag cattgccctt tacggcattt tctttgatac caacaaatgg gacgtcaaac
    15301 aggagtcgga acctaccctc gaggagatcg cgaaattcct caagggcaac ccttccatct
    15361 caatcctggt agtgggtcac accgataacg tggggacctt cgatttcaac ctggaacttt
    15421 ccagaaaaag ggcaggctcc gttgtttcgg cccttgcaga aaaattcggt atcgacagga
    15481 aacggctttt cccggtggga gtatctttcg cctcccctgt ggcttccaac agcaccgaag
    15541 agggaaggtc aaagaaccgc cgggtggaac tggtggaaaa ctgacggacg gacggacagg
    15601 atgggctcct gaaggagccc atccccggac gtaactcctc tgaaacccct tagtgattaa
    15661 aacatagagg cgagacatgg atccagggac tccatcgcac ccttcctttt gtgaacatgg
    15721 cagtcgccga ttatacttgc catgtgtcgg gtttaaggac ccgatcttaa taaaggagtg
    15781 atgtgcttgt tcgctctttt gaatccttat tcgatcattg agattttatt ggtggtcctg
    15841 tccggcatcg ttattgccac cgcttttttg atgtttcgta gcacccgttc cgg
//
LOCUS       DAOTIQ010000044        15853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4454_length_15853_cov_7.012027, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000044 DAOTIQ010000000
VERSION     DAOTIQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_15853_cov_7.012027"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(606..2210)
                     /locus_tag="P5541_06585"
     CDS             complement(606..2210)
                     /locus_tag="P5541_06585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV71686.1"
                     /translation="MRKNAFVLHNISAWTADPLKPEADALIVDGEKISAVGGLDEIMS
                     HPASANAEWIDAGGATLIPGMTDSHLHLTALSRQRAATSLFEATSKENLLEMIRAKAE
                     KMAPDEWIYGVRFDNSRWEDTSLPTIKELDGLGIPNPVLLLRVCAHIHVVNSRALREA
                     GLSSEDLSGAGVVRDDSGCFTGTLHESFAQPVVEAMKRNSMSGPGEASSLRETMIQLV
                     SEGITSIHTCSAASYGLEENIEAYKHLHDSGQLPLRVTLYADHPVPEWSRSGEGDDWF
                     RYGGKKFFLDGSLGGRTAAMTFPYKDEQSTKGMLNMDTPEFCSMVRMEHEMGNQVQVH
                     AIGDAAIDQFIEAVEGVTDLPPLSGGLRHRIVHVQVCRPDQMKKLKRVGAVCDIQPVF
                     VPSDIHITEPRVGKERLDWAYAWKEMLRMGLLLTGSSDAPVEPTNPWRGIWAAVNRVD
                     DNGNPAGGWLPEQKLTLDEALALYTTNPPRAIGTAGRFGMIRAGMAADLVILDRNIHR
                     TPPEKLLEVKPAMTMVGGKIRFVGEG"
     gene            2652..2825
                     /locus_tag="P5541_06590"
     CDS             2652..2825
                     /locus_tag="P5541_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HRV71687.1"
                     /translation="MKEQAICVVCECAIDFAPGTLKGEILVCPDCGTELEVVCTTPPG
                     LAEAPQVEEDWGE"
     gene            2818..3708
                     /gene="lysX"
                     /locus_tag="P5541_06595"
     CDS             2818..3708
                     /gene="lysX"
                     /locus_tag="P5541_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HRV71688.1"
                     /translation="MSKDRIRILYTRLRKEEKLLAEASQRSGVDFTLEDVRDWVMPQE
                     ICGNDIFLCRCIGHWQNLEVARILHHAGARTVNAPEIMEICGDKIATSSILKKAEIPQ
                     PGFLVAFSRDAAVEAAGRLGYPVVFKPSVGSWGRLLAKVGDSDMAESIAEHKDHLGPA
                     HQVFYIQEFIQKPGFDIRAFIVGGEPVCAIRRYSDHWITNTARGGKTEEHPLDKDLRE
                     ILCRVHDVIPGDFLAVDCFLTDSGLLVNEVNDGAEFRNSIDPTGRDIAGDVIALCSSL
                     LKRKNLKRKEKKYSGPGTSV"
     gene            3801..4856
                     /gene="argC"
                     /locus_tag="P5541_06600"
     CDS             3801..4856
                     /gene="argC"
                     /locus_tag="P5541_06600"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRV71689.1"
                     /translation="MNSERSFSVLIYGAGGMVGGELLRIITGHPRFKIAGAVSARKVG
                     KPLAMIHPHLRHACPDTVFISPEAARDTPADIAFTSLPHGSASESVAEILLRGIPVVD
                     LSADFRLKDLGRYERWYGGPHPRPDILDVAVYGLPELHRKELPGASIVSGVGCNAAAS
                     ILALYPFARSGLIKEALLDVRAGSSEGGSKANQGSHHPFRSRALRIVEPFSHRHLAEV
                     SQELSLPEENLTMRLTAVENVRGVQVCAEIFLHEHLREADLWKLFRESYAEEPFVDLC
                     PARPMHLRFPDPRLVTGSNRVLIGFSTTGKGTRCLVVVALDNLMKGSAGSAVQSANVV
                     LGIPETTGLLMQPVYPV"
     gene            4874..5710
                     /locus_tag="P5541_06605"
     CDS             4874..5710
                     /locus_tag="P5541_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HRV71690.1"
                     /translation="MPTGVIKIGGGEGNNPSRVLKEIATRHDRGESWVLVHGVSSQMD
                     SLCRAVGIRPHYVISPSGFRSRYVGDEEMELFEAAALAETSRLCSFLVTLGVPAMPLD
                     PVTTGFITGETKNCIRELSGGKVRVLRGNRSGKVVGVDVSPLNWMLKRGYLPVVPPLG
                     RDRETGRGMNIDGDRAAAAIAGEIGASIMVLLTNVPGLMRDPDDRATLIPEIRSNEWK
                     EAEAVARGNMKRKVLACREALERGVSEAVISDSRVELPLASATEGGGTRFCRADCTVI
                     AV"
     gene            5677..6798
                     /locus_tag="P5541_06610"
     CDS             5677..6798
                     /locus_tag="P5541_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV71691.1"
                     /translation="MQSGLYGNRGLTVGGGEGSFIFDSEGRRYIDFFCGHGSSLFGHA
                     HPVLVNALKRAAEVPWSTGAGLIEKVRVEASEALMKFLPGFRVYWGNSGTEAIEAALK
                     ICALNRPGRARFLALRRSFHGRTAGALSLTFNPRYREPFSNLLFSAEHFRAEDLPGAI
                     DERTSAVFIESVQGEGGVHLLSRGTGKAITDRCRETGALLVADEIQTGFGRCGSFLAS
                     PDLGIQPDVVCLSKGVAGGLPAGVTLWREELSDFLPNTQGSTAGGNPLVCAVALAALD
                     LLEKERYPARATENGAYFRELIEEIDSPQIVEVRGIGLLTGVEVKVKAPVLVRKLQER
                     GVLALAAGPLVVRFLPPFVAEKEQFLSTVRVFREVLEDL"
     gene            6795..7865
                     /locus_tag="P5541_06615"
     CDS             6795..7865
                     /locus_tag="P5541_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV71692.1"
                     /translation="MKRSTEISLLAELVQTPSTSGNEGAASNVLMERLPLFGWERVEK
                     DQVGNVVARRGSGAQELVLLGHIDTVPGGPCFGLHGQVLRGRGSVDAKGPLCSFIIAG
                     AAVHIPEAWSITVIAAVGEETDSRGIKHRIDLHKPCACIVGEPTGGDGVVLSYRGRIL
                     CELHGEDRGGHRSWSPGPLTATVRAASEIFNWIESIDPDLPLTSRLSGSVVSMSGEES
                     SGRSASVVLDIRLPLGRKIGEVIEKIQNISCSFGVSAEIHESVAAHGVRPHDPVVGAL
                     RSAIISSGSDPRLLKRSGSADFNMAAAWNCPMAVYGPGDSLLEHTEEEQIEIDEYLAS
                     IDILRSAIPNVFRILGTERVQP"
     gene            8091..9422
                     /locus_tag="P5541_06620"
     CDS             8091..9422
                     /locus_tag="P5541_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRV71693.1"
                     /translation="MKITKEELLKKAHQPARDAMELHPFYRGKMQTAPKCCIRDINDF
                     AIWYTPGVAEPCKAIKEDPELAWEFTNRANTIAVVSDGTRVLGLGDIGPEAGMPVMEG
                     KALLFKYLGGVDAVPLCLDTRDPDKLIETVRLLAPSFGGINLEDISQPKCFYILDELR
                     KECKIPVWHDDQQGTAAVNLAGLYSGLKITGKKIEDIRVGFLGAGAASVATARILISA
                     GVDPARVLMADSRGILHGGRTDLDQKYDVKKHFAEITNPKGISGGPEVLFEGMDVVYC
                     YTKPGPDVVKKEWVAKMARDAMLFACANPVPEIWPWEAKEAGVRVVATGRSDFPNQVN
                     NSLIFPAVFRGTLDVRATTITDEMCIAGAKTLSDCAEKIGMTEEYLIPTMDQSWVYPE
                     VAASVGMKAIEQGLARVKMTREELLATAAEKIGYAQGMTRCLTDNGFIKLP"
     gene            complement(9426..11054)
                     /locus_tag="P5541_06625"
     CDS             complement(9426..11054)
                     /locus_tag="P5541_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV71694.1"
                     /translation="MPTRVAVVLAYFAGILIVGLLARSRAKSTPEDYFLADRKLGPLL
                     LVATMAATNFSAFTVFGTSGAGYRDGYSFFPIMGFGTGFMALSFWVLGRKIRQIGIKH
                     NTITPSELIGSIYKSPFLSMLFAIVMIAFTIPYLALQPMAAGYALEELLGLPYMWGCC
                     LVTSIIVAYTLCGGMRAVVWTDLLQGTFMALLLLVALGTVSLHHGGFIAANRAVMESV
                     PELFSRPGGAGTYTLGIWFSYMLLWFFCDPMFPQLFQRFFSARDERALSLTMLLYPLI
                     CSFIFFLPVSMGVLGHLSFPGLTGSQSDRIIPMMLTAISGDFMASLIIAAALAALMST
                     MDSQLLTLSSIFSRDILPVFSRKTRHGDLAGKVFVVVLASAGLVLAYRPPETILILAT
                     QTFSGLAVLFPTVFFGLYLKRVFPLSAILSIATGEATLLGFYLGWLSPGPFLPVIWVM
                     LVAFSSYLAIHFATAWKLGIEKTGSADWLKNPYVFLSAGLFVLAMDIWNWNRSLPTLW
                     SIPLWVWYFGALSVLQTLMMFFMTRRKKDRVHNL"
     gene            complement(11132..12487)
                     /gene="mgtE"
                     /locus_tag="P5541_06630"
     CDS             complement(11132..12487)
                     /gene="mgtE"
                     /locus_tag="P5541_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRV71695.1"
                     /translation="MKERLKRLNGIIDSFLLEHRFPEIKEALSEMEPADISAILAGTT
                     RARMVLVFRLLPKDLAIEVFEYMEGREREELLNSFTDREAAEIIEEMSDDDRTELLEE
                     LPAKTVRKLMQVLSPEERKIANRLLNYPEDSAGRIMTAEYIGLKASMTATESLQHIRR
                     EARSKETIYTCFVMDAQRHLLGVVNLEDIIMAQPDTLISDIMDKDPVRVATTTDQEQL
                     ARIMSRYDLHVIPVVDLEERLVGIVTFDDVLDIVEEEATEDFELMAGIRPVEDSYLQT
                     GILTLARKRFTWLLVCIITQAVTSTILQNNEAVLQNVIALAFFIPFLIDSGGNTGTQS
                     STLMIRGMTLGEIKWGNIGLVFMRETATGLLLGTALALVGIIRAMTMGIGMDVAITVA
                     ISLVAVVMIGNLMGVILPFAARALRFDPAIVSGPFITTVVDVSGLIVYFGIASRVMGI
                     G"
     gene            13347..>15853
                     /locus_tag="P5541_06635"
     CDS             13347..>15853
                     /locus_tag="P5541_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV71696.1"
                     /translation="MAQLEHIEAIEKRLWSAADTLRSNSNFASNEYFLPVMGLIFLRH
                     AYSRFLAVKSSVEETLPRRGGKARPLSKEDFSQKSAIFLQPKAQFDHLVALSDSSDRA
                     RAIIEAMESIESDYENLRGVLPKSEYQELDNDVLGQLLRTLNPDELKKVNGDVFGRIY
                     EYFLTQFADLKAHDGGEFFTPVSLVSLIANVLEPENGVVLDPACGSGGMFVQSARFVE
                     RRRENPVDRLTFYGLEKNATTIRLAKMNLAVHGLEGDIQKAITYYEDPHELLGKANFV
                     MANPPFNVDEIDADKVRTDPRLPFGLPGVNKGGKVSNGNYVWISYFYSYLNENGRAGF
                     VMSSQASSAGGGEAKVRQKLVETGDMDVMIAIRPNFFYTRTVPCELWFIDRGKPDQPS
                     PLAPLPEGEEGNSYHYRGGYNFSGLVELAREFRKNQTPAEDCFWSIVQNRKFLGLKFR
                     RQHQVGDYVVDFYCKEASLAVELDGPVHDLQTKRDAKRDAYLKSQGVHVLRIKNGDLL
                     EKTQATLEQLANRIFSSSEIPLPLGEETGEGDVALNHKGNVLMIDARNVYRKVTRKIY
                     DFSPEQEQNILAIVWLYRGQTEKYLDLVGGYCRRMLAEGEGCFAARDDSGEKTKPLPS
                     FIASLTALREALQAFLANPSKDVAFEELQKELEEAVSVFKGDVETFREHLSEQQSTWE
                     GQKITNGELKKAVDRLVSLGESSRDLVKQTDLIFKIASRFMEAIPQAFAPRQSSGGNG
                     IGQMKRQLRDLDVARALAVDQLKQVRYFWKQARWLTERFPEAKLCDVPGLVKLVDKAE
                     IEANDWSLTPGRYVGVAPEEVDENFDFEETLREIHVEL"
BASE COUNT         3643 a         4077 c         4612 g         3521 t
ORIGIN      
        1 aaggcaacca gggagcggtt tgagaggttt gtgtaattgg atcgtaacgc gtgacgcata
       61 gaagccgtga atcgtgaatc gtgaatcgtg aatcggctga aaccagggaa acaggagact
      121 ggcatcagga ggcaggaaag caggagcaaa ggcgtgaacg gtcaacagtg aacggtgaac
      181 gggaagggcc ggggccggag agaagtcttt accgaaggct caggagccgg tgagtttcgc
      241 agccgtcgcg gaggggcagg gaaagcacac ctgccggggg ttttcggcac tgtgcggtcg
      301 ccccggagca ggcagagaaa cgaacgcttt caggcgagtt gcctctcgga aagacttcac
      361 tcagggcccc ggccagtatc ctggtggaat ttgaaccaga ttcccgccgg taggcaaagg
      421 gcgcggaacg tccgcctgct ggcggattcc gctttgctgc gctcagaatg acaagcaaaa
      481 gcaagaagac tggagactgg agacgggata ctggaggcag gaagggcaag ggccttaccc
      541 tcgccgcttc gcttcacacc gttaaccgtt cacagttgac tgtttacgcc tttgacccat
      601 tcgagtcacc cttcaccaac aaaccggatc tttccgccga ccatcgtcat tgcgggcttt
      661 acttcgagca gtttttccgg aggggtcctg tgaatgttgc ggtcgaggat gaccaggtcg
      721 gcggccattc cggcccttat catgccgaat ctcccggctg ttccgatggc gcggggcggg
      781 tttgtcgtgt acagggccag ggcctcgtca agggtcagtt tctgttcggg cagccaccct
      841 ccggcggggt ttccattgtc atcgaccctg ttgactgccg cccagatgcc ccgccagggg
      901 ttggttggct ccaccggcgc gtcgcttgac cctgtgagca gaagtcccat ccggagcatc
      961 tccttccagg cgtaggccca atccagcctt tctttcccta ctcggggctc cgttatatgg
     1021 atatcgctgg gtacgaacac gggctggata tcgcagaccg ccccgactct tttcagtttt
     1081 ttcatctgat ccggcctgca aacctggaca tgcacgatcc tgtgccgcag ccctccggaa
     1141 agagggggaa ggtccgtcac tccctcgacg gcctcaatga actggtctat ggccgcatcg
     1201 ccaatggcat ggacctggac ctggttgccc atttcgtgtt ccatccgaac catggaacag
     1261 aactccgggg tatccatgtt cagcatgccc ttcgttgatt gctcgtcctt gtagggaaat
     1321 gtcatggccg ctgtccgccc gccaagagag ccgtccagaa agaacttttt gcccccatag
     1381 cggaaccagt cgtcgccctc tccgcttctg ctccattcgg gaaccgggtg gtcggcgtag
     1441 agggtgaccc gaagcgggag ttgtccgctg tcgtgaaggt gcttgtaggc ctcaatgttt
     1501 tcctccaggc cgtaggacgc agcgctgcat gtatgaatgg aggttatgcc ctccgaaacc
     1561 agttgtatca ttgtttcccg cagagaactg gcttctcccg gaccgctcat ggagttccgt
     1621 ttcattgcct ccacgaccgg ctgggcaaaa ctctcgtgca gggtccccgt aaagcagcca
     1681 ctatcatcgc ggacgactcc cgcccccgaa agatcttccg aagaaagccc cgcttcccga
     1741 agggctctgg agttgacaac gtgaatatgg gcgcagactc taaggagaag gaccgggttg
     1801 ggaatgccaa ggccatccag ttcttttatt gtcggaagag atgtgtcctc ccagcgggaa
     1861 ttgtcgaagc gcaccccgta tatccattca tcgggagcca tcttttcggc ttttgcccgg
     1921 atcatttcca ggagattctc cttcgaggtc gcctcaaaga gcgacgtggc cgccctctgc
     1981 cttgaaagcg ccgtgaggtg caggtgagag tccgtcatgc cgggtatcaa ggttgcccca
     2041 ccggcatcta tccattcggc attcgctgag gccgggtggg acatgatctc atccagcccg
     2101 ccaacggcgg aaattttttc tccatcaacg ataagtgcat cggcctcggg tttaagagga
     2161 tcagcagtcc aggcggagat attgtgcaag acaaaggcgt tttttctcaa gtcgtttctc
     2221 ctctcagtat ttaacgatgc catgatatca cttgcagact catgaagcag atggcggagc
     2281 atgtcaactc gatgacttga ctttggaaga aaaaatctct acacttccgt gtaacaatcg
     2341 aaatggttcg atccagtgta gcgaacaaag gcgatgaagc ggggcgcttt cccgggaaca
     2401 agctcttcag agagggatct ccaccggctg aaagagatcc cgggcatacc ggaaaagccc
     2461 aaaacccgtt gagccggatc ggaaagggga aacttcccgg agtacgaatc ccggtttggg
     2521 acaccgtcat cagtcccgag aggagtgcat gcgaatgcgc tcaaaaaggg tggtaccgcg
     2581 ggttaatggc ccgtcccttt agtggggcgg gccgtttttt atttcaagcc aggaagggaa
     2641 aggtgaaagg gatgaaagaa caggcgatat gtgttgtttg cgagtgtgcc attgattttg
     2701 caccgggaac tttaaagggc gagatccttg tctgcccgga ttgcgggact gaacttgagg
     2761 ttgtttgcac gaccccccct ggtcttgccg aagcgcccca ggtggaggag gactggggtg
     2821 agtaaggatc gtatcagaat cctttacacg aggcttcgca aagaggaaaa gttactggca
     2881 gaggcctcgc agaggtcagg cgttgacttc accctggaag acgtcaggga ttgggtaatg
     2941 cctcaggaga tatgcggtaa cgatattttt ctttgccgct gcattggtca ttggcaaaat
     3001 ctcgaagttg cgaggattct ccatcatgct ggagcaagga cggtcaacgc gccggagatc
     3061 atggagattt gtggcgacaa aatagccacc agctcgatcc tcaaaaaggc cgaaatcccc
     3121 cagcccgggt tccttgtcgc gttttccaga gatgcagctg tagaggctgc cggtcgactt
     3181 gggtaccccg ttgttttcaa gccttccgtc gggagctggg ggcggcttct tgccaaagtt
     3241 ggtgattccg acatggcgga gtcaatcgca gagcacaagg accatcttgg accggctcac
     3301 caggtttttt acatccagga attcattcag aaaccgggtt ttgacatcag ggctttcatt
     3361 gtggggggtg agcctgtgtg tgctatcagg agatactcgg atcactggat caccaacaca
     3421 gcccgtgggg ggaaaacaga agaacaccct ctcgacaaag atctccgtga gatactctgc
     3481 cgggttcatg atgtcattcc gggagatttt cttgctgtgg attgtttttt gaccgattcc
     3541 gggctgcttg taaacgaagt aaatgatggc gccgagttcc gcaattccat tgacccaacg
     3601 ggcagggata tcgctggtga tgtcatagcc ctctgttcgt ccttgctcaa gcgtaaaaat
     3661 cttaagcgaa aggagaagaa atattcaggt ccggggacat ctgtgtaggc taccaaaaaa
     3721 gcagcacaaa gatcacagaa acccggaccc gtggaaagcc tttccacggg tcattttttt
     3781 tgggaagagg tgttagtgac atgaattctg aaagatcatt ttcggtattg atttacgggg
     3841 cgggcgggat ggttggggga gaacttttgc gaattatcac cgggcacccc cgatttaaaa
     3901 ttgccggggc ggtttctgcc cgcaaggtgg ggaagcccct ggccatgatc catccccatc
     3961 ttcgccacgc ctgccccgat acggttttca tttcccctga ggctgctcgg gatactcctg
     4021 ccgacattgc cttcacgtca cttccgcatg gttccgcaag tgaaagtgtg gctgagattc
     4081 tcctgagagg gattccagtg gtcgacctgt cggccgactt caggttgaag gatttggggc
     4141 ggtatgaaag gtggtacggt ggcccccacc ccaggcccga tattcttgat gttgcggttt
     4201 acgggttgcc cgaactccac aggaaagagc ttcccggggc ttcgatcgtt tccggcgtcg
     4261 ggtgcaatgc cgcagcctcc attttggcac tatacccatt tgctcgcagc ggactgataa
     4321 aggaggccct tcttgatgta agggcaggtt catcggaagg tggctcaaag gcgaatcagg
     4381 gcagtcatca cccgttcagg agtcgtgccc tgcgtattgt cgaacctttc tcccacagac
     4441 atctcgcgga ggtttcccag gagctgtccc tgccggaaga gaatttgact atgcgcctga
     4501 ccgctgtaga aaatgttcgg ggtgtgcagg tctgtgccga gatttttctg cacgagcatc
     4561 tcagggaggc cgacctttgg aagttgttca gggaatctta cgcggaagaa ccctttgtcg
     4621 atctttgccc ggctcgacct atgcatctgc gttttccgga tccccgtctg gtcacgggaa
     4681 gcaacagggt tttgataggt ttttctacaa cgggaaaagg gacccggtgt ctggttgttg
     4741 tggccctgga caacctcatg aagggctctg ccgggagcgc tgtccagtcg gcaaacgtgg
     4801 ttctcgggat tccagaaaca accggattgc tcatgcagcc ggtatacccg gtctagtaaa
     4861 gggagtgaag agaatgccca caggagtaat caagatcggg ggaggcgagg gcaacaaccc
     4921 ctccagggtg ttgaaagaga ttgcaacaag acacgaccgc ggagaaagct gggtgcttgt
     4981 tcatggagta agttcccaaa tggattctct ttgtcgtgcc gtggggatca ggcctcacta
     5041 cgtgatcagc ccctccgggt tcagaagcag gtacgtcggc gatgaagaga tggagttgtt
     5101 tgaggctgcc gcccttgctg agacaagccg tctctgctcg tttcttgtaa cactgggagt
     5161 gcctgccatg ccgttggatc cggttaccac ggggttcatt accggcgaaa caaaaaattg
     5221 tatcagggaa ttatccgggg gaaaggttcg ggttctgcgc ggcaacagga gcggcaaagt
     5281 cgtgggtgtg gatgtctccc cgctgaactg gatgttgaaa cgggggtatt tgcctgttgt
     5341 tccaccactt ggccgagaca gggaaaccgg caggggaatg aatattgacg gagacagggc
     5401 tgctgcggct atcgcgggtg aaattggggc cagcataatg gtccttctta caaatgtccc
     5461 gggtctgatg agggatcccg acgacagagc gacgttgatc ccggagatta ggtcgaatga
     5521 atggaaagag gccgaagcgg tggcaagggg aaacatgaaa agaaaggttt tagcttgcag
     5581 ggaagccctt gaaaggggag tttcggaagc ggtcatatcc gatagtcgtg tcgaactccc
     5641 tcttgcaagt gccactgaag gaggggggac aaggttttgc agagcggatt gtacggtaat
     5701 cgcggtctga cggtcggggg tggagaaggg tctttcatct tcgatagtga ggggagacgc
     5761 tacatagact ttttctgcgg gcacgggtct tctctttttg gccatgccca tccggttctg
     5821 gtcaatgccc tgaaaagggc tgccgaagtt ccctggtcga ccggtgccgg ccttatcgaa
     5881 aaggtaaggg ttgaggcgtc cgaagcgttg atgaagttcc ttccgggatt cagggtctac
     5941 tggggcaata gtgggacaga ggcaattgag gcagccctca agatttgtgc cctcaaccgc
     6001 cccgggaggg cccgatttct tgccctgagg cgaagtttcc acggccggac cgcgggcgcc
     6061 ctcagcctga cattcaatcc cagataccgg gagccttttt ccaatctgct gttctccgca
     6121 gagcatttcc gggcagaaga tcttccggga gccatagatg agaggacctc tgctgtgttc
     6181 atcgaatctg tacagggtga gggtggagta cacctgttgt ccaggggaac aggaaaggcc
     6241 attaccgaca gatgcagaga aacaggtgcg cttctggtgg ctgatgagat ccagactggc
     6301 tttggcaggt gtggttcatt cctggcaagt ccggatttgg gtatccagcc tgatgttgtt
     6361 tgcctttcca agggcgttgc gggggggtta ccggcaggtg ttaccttgtg gcgcgaggaa
     6421 ctgagtgatt ttctgcccaa tactcaaggt tcaacggccg gggggaaccc tctggtttgt
     6481 gccgttgccc ttgccgccct ggatctcctg gaaaaggaaa gatacccggc ccgtgcgacg
     6541 gaaaatgggg cctacttccg ggaacttata gaagagatcg atagtccgca gatagttgag
     6601 gtgaggggga ttgggctttt gacaggtgtc gaagtcaagg tcaaggcgcc ggttctggtc
     6661 agaaaactcc aggaacgggg ggtccttgct cttgccgcag gccctttggt cgtgcgcttt
     6721 cttcctccgt ttgtggcaga aaaggagcaa ttcctttcaa ctgtgcgtgt atttagagag
     6781 gtgctggaag atctatgaaa agatcgactg agatatccct gctggccgaa ctggtccaga
     6841 ctccgagtac cagcgggaat gaaggtgcag cgtccaatgt tttgatggag cggcttccct
     6901 tgtttggttg ggaaagagtc gaaaaggatc aggttggtaa tgttgtcgcc aggaggggaa
     6961 gcggagccca agaacttgtg ctgctggggc atattgatac cgtcccggga ggcccttgct
     7021 ttggccttca cggtcaggtg ctgaggggaa ggggttcggt tgatgccaag ggtcccctgt
     7081 gctcctttat tatcgcgggg gcagcggtcc atattccaga ggcatggtcg ataaccgtta
     7141 tagccgccgt aggagaggag accgattcaa gggggataaa acacaggata gatctgcaca
     7201 aaccctgtgc ctgtatcgta ggggagccta caggagggga tggcgtggtc ctttcctaca
     7261 gggggcgcat cctttgcgaa cttcacggtg aggatagagg ggggcaccgt tcctggagcc
     7321 ctggacccct tactgccacg gttcgggcag cttcggaaat ttttaactgg atcgagagca
     7381 tagatcccga ccttcctctt acgtctcgtt tatcaggttc ggtggtttcc atgtcaggag
     7441 aagaatcatc cgggagatct gcgagtgtcg ttctggatat acgtctgccc cttggtcgaa
     7501 agatcgggga agtgattgaa aagatccaga acatatcctg ttctttcggg gtttcggccg
     7561 aaatccatga atcggttgcg gctcacgggg tcaggcccca cgaccccgtg gtgggagcat
     7621 tgagaagcgc cattatatcg tccggatcag atccgaggct tctcaagagg agcgggagcg
     7681 ccgatttcaa catggcagca gcctggaatt gtcccatggc cgtgtacggt ccaggcgact
     7741 ctctactcga gcatacagag gaggaacaga ttgaaattga tgaatatctg gcgtcaatag
     7801 acattttgag gtcagctatt cccaatgtgt tcaggatact gggaacagaa agggttcaac
     7861 cctagagatc cacgggcagg aaatgccgtg cgccagacct ttcataaacg actcaagggc
     7921 tatctttctt atgctacggg agggctattc cccctcccgg gaatattaca gcaaaatcga
     7981 ggtccagccc tgaaaccgtt gaggaatttt atcgcttttg cggatagaat tatccctgtt
     8041 gcggaaatcg taacattcag agggattttt taaagcctgg aggggacgtt ttgaagataa
     8101 ccaaggaaga actgctaaaa aaagcgcatc agcctgcaag ggatgcgatg gaattgcacc
     8161 ccttctacag gggcaagatg cagaccgccc caaagtgctg cataagggac atcaacgact
     8221 ttgccatctg gtacacgccc ggagttgccg agccgtgcaa ggccataaag gaagacccgg
     8281 aactggcctg ggaattcacc aacagggcca acaccatagc agtggtgtct gacgggacga
     8341 gagtcctcgg tctcggcgat atcggacccg aggctggaat gccggtcatg gaggggaagg
     8401 ctctcctatt caaatacctc ggcggggtcg acgccgttcc tctctgtctc gatacaaggg
     8461 atcccgacaa actgatcgag accgtcaggc ttcttgcgcc ttctttcggg gggataaacc
     8521 tggaggatat atcccagccc aagtgtttct acattcttga cgaactccgg aaagagtgca
     8581 agatccccgt ctggcatgat gaccagcagg ggaccgcggc ggttaacctg gcgggtcttt
     8641 acagcggcct caagataacc ggcaagaaga tcgaagatat cagggtcggg ttcctgggtg
     8701 ccggggctgc aagtgttgcc acggcaagaa tactcataag cgcgggggtg gatcccgcca
     8761 gggtactcat ggccgacagc aggggcattc ttcacggtgg aaggaccgac ctcgaccaga
     8821 aatatgatgt caagaagcac ttcgccgaaa ttaccaatcc gaaaggcatt tcggggggac
     8881 cggaggtgct tttcgagggg atggatgtgg tctactgtta cacgaagccc gggccggacg
     8941 tagtgaagaa ggaatgggtg gcgaaaatgg cccgggacgc catgcttttt gcatgcgcca
     9001 accctgttcc ggagatatgg ccgtgggagg cgaaggaggc cggagtcagg gtagtggcca
     9061 ccggaaggtc cgactttccg aaccaggtca acaactcgct gattttccct gccgtattcc
     9121 gggggaccct ggatgtgagg gcgacaacga tcaccgacga gatgtgtatc gccggcgcga
     9181 agactctttc tgattgcgcg gaaaagatcg ggatgactga agaatacctt atcccgacga
     9241 tggaccagtc ctgggtctac ccggaggtcg ccgcctcggt ggggatgaaa gctatcgaac
     9301 agggccttgc tcgggtaaag atgaccaggg aggaacttct cgcgactgcg gccgaaaaga
     9361 tcgggtacgc gcagggaatg acgcggtgtc tcaccgacaa cgggttcata aaactgccgt
     9421 gagtcttaca ggttgtggac ccggtccttt tttcgccggg tcataaagaa catcatgagt
     9481 gtctggagga ccgaaagggc accgaagtac catacccaca gggggatgct ccagagggtt
     9541 ggaagagacc tgttccagtt ccagatgtcc atggcaagca cgaaaaggcc tgcggagagg
     9601 aagacgtagg gattcttgag ccaatcggcc gatccggtct tctcaatgcc cagtttccag
     9661 gcagtcgcga agtggatcgc aagatatgag gagaacgcga ccagcatgac ccagatcacg
     9721 ggcaggaaag gcccgggtga taaccatcca aggtagaagc caagcagggt tgcctcgcct
     9781 gtcgcgatcg acagaatggc cgaaagaggg aaaacccttt tcaggtagag accgaaaaaa
     9841 accgtgggga agaggaccgc tagccccgaa aaagtctggg tagccagtat cagtatcgtt
     9901 tccgggggtc tgtacgccag gacaagaccg gcagaggcaa ggaccacgac gaagactttt
     9961 cccgcgaggt cgccatgtct ggtctttctg ctaaatacag gaagtatatc cctgctgaat
    10021 atggaactca gggtaagcaa ctgggaatcc atggtggaca tcagtgctgc cagtgccgcc
    10081 gcgataatca gcgacgccat aaaatctccg gagattgccg taagcatcat cgggattatc
    10141 ctgtcgctct gggagcccgt aagtccgggg aaggaaaggt gtccaagaac acccattgaa
    10201 acgggcagga agaagataaa ggaacatata agcgggtaaa ggagcattgt tagcgataag
    10261 gctctttcgt ccctggcgga aaagaatcgc tggaaaagtt gggggaacat ggggtcgcaa
    10321 aagaaccaca ggagcatgta actgaaccat atacccagag tataggtccc cgctccgccc
    10381 ggccgcgaaa agagttccgg gacggactcc attaccgcgc gatttgccgc aatgaagcct
    10441 ccgtgatgca gtgaaaccgt tcccagggcc accagcagga gcagggccat aaaggtcccc
    10501 tgcagcaggt ctgtccagac tacggctctc atccctccgc agagggtgta ggcgacgatg
    10561 atagaagtaa caaggcagca accccacatg taagggagtc cgagaagttc ctcaagagcg
    10621 taaccggctg ccattggctg taatgccagg tagggtatcg tgaaggcgat catgactatg
    10681 gcgaaaagca tcgaaaggaa agggcttttg tagatcgaac cgatcagttc ggacggggta
    10741 atcgtattgt gttttatccc gatctgtctg attttccggc ccaggaccca gaaactgagg
    10801 gccataaagc cggtcccaaa acccattatc gggaaaaaag agtacccgtc ccggtatccg
    10861 gcaccggagg ttccgaaaac ggtgaaggct gagaaattcg tcgcggccat tgtggcgaca
    10921 agcaacaatg gccccagttt gcgatcggcc aggaaatagt cctcgggtgt ggattttgcg
    10981 cgggagcggg caagcaaccc aacaatcagt atccccgcga agtaggcgag caccacagca
    11041 acccttgtcg gcatgcaata acctctgtct ctaatctctg tatttactat agtgccatat
    11101 ctcccgcggc gtcaaatgga gatggaaaag gttatccgat ccccattaca cggctggcta
    11161 tcccgaagta gacaataagt ccgcttacat cgacgacggt cgttatgaac gggcctgata
    11221 ctattgcggg atcaaagcgt agcgctctgg ccgcgaatgg gagaatgact cccatcaggt
    11281 tcccgatcat tactaccgcg acaagggaga tggcgacggt aatggccacg tccattccta
    11341 tccccattgt catggccctg atgatcccga ccagggccag ggctgttcca agcaagagtc
    11401 ccgtggcggt ctcccgcatg aaaaccagac caatattgcc ccattttatc tcgccgaggg
    11461 tcattcccct gatcataagg gtagatgact gggtgcccgt gttccctccg ctatcgatca
    11521 ggaacggaat gaagaatgcc agagctatca cgttctgcag gacagcctcg ttattttgga
    11581 gtatggtgga ggtcactgcc tgagttatta tgcataccag aagccatgtg aagcgttttc
    11641 ttgccagtgt caggatcccc gtctggaggt agctgtcctc gacgggccgg atgccggcca
    11701 taagttcgaa gtcttccgtg gcctcctctt cgacgatgtc aaggacatca tcgaaggtta
    11761 ctattccaac gaggcgctcc tcaaggtcga ctacgggaat aacatgcagg tcgtagcgtg
    11821 acatgatccg ggcgagttgt tcctggtctg tggtggtcgc gaccctcaca gggtccttgt
    11881 ccatgatgtc cgaaatcagc gtatccggct gtgccattat gatgtcctcg aggttaacca
    11941 cccccagaag gtggcgttga gcgtccatca cgaaacaggt atagatggtc tccttgctcc
    12001 tggcctctct cctgatgtgc tgaagagatt ccgtggctgt catggaggcc ttgagcccta
    12061 tgtattcggc ggtcattatc cttcccgctg agtcctcggg atagttcagg aggcgattgg
    12121 cgatcttcct ctcttccggc gaaaggacct gcatgagttt cctgaccgtc tttgccggca
    12181 gctcctcgag aagttctgtc cggtcgtcgt cggacatctc ttcaatgatc tctgccgctt
    12241 ccctgtcggt gaaactgttc aggagttctt ccctctctcg cccttccata tattcgaaga
    12301 cctcgatggc aagatccttg ggcaggagtc ggaataccag gaccattctt gcccgggtgg
    12361 tcccggccag aatggcgctg atatcagcgg gttccatttc tgaaagggcc tctttgattt
    12421 ctgggaaccg atgttcgagg agaaaggaat caataattcc gtttagacgt ttcagacgtt
    12481 ctttcatgaa gccgtcaccc ccacgcgcca caaggcttag gttggacctg aaacagaaca
    12541 ggccgcaact gccctggcac taaaaatcga cgattttccg gtactttggt tctcttgagg
    12601 gtgttcggga gggaaatttg aacggtgatg cgtggaaggg aaagttatgt ccgcgtcagg
    12661 ctcgccccgg gatcaccctc ttttctgctg ggactgctgg taaaggaata ctttggcttt
    12721 cttccatctg aatctccttt aaaggtctta cccataattt ccaatcaatg ctaaaccacc
    12781 agagacacaa ggtcaacaaa gggaaaaaac caggcaggtg tgccgttgac cgtttacgtc
    12841 tttgattttg cctgtcatcc tgaggagcgg aagtgacgtg aggatctggg tttgtctttg
    12901 gctttttcga ttcacgactc acgattaacg actcgcgggg cttgcccctc ccgttcaccg
    12961 ttcacgcagt tgaattttcg gcccttgccc ttgtttttcc attcgtctca cttctcacgt
    13021 ctaaccccct tgggcgggct ccggtcgtcc agcgaagaag atgccataga cgaatccact
    13081 atctgtaaaa ccatccagaa taggtggacc gcctcccgga gaccccttgg gcgggctccg
    13141 gtcgtccagc gaagaagatg ccatagacga atccactatc tgtaaaacca tccagaatag
    13201 gtggaccgcc tcccggagac ccctcacgcc cctgcacttg cctttcccct ttggccttgc
    13261 tttatttgtc gactatgttg tattttactt gtaaaaccgc cgcgagttcc ctgtgctgtc
    13321 aagatactac caacaatgga ggagacatgg cccaactcga acacatcgaa gccatcgaaa
    13381 aacgactctg gagcgcggcg gacacgttgc ggtcgaactc caactttgcc agcaacgagt
    13441 atttccttcc cgtaatgggg ctgatcttcc tgcgccatgc ctacagccgc ttcctggctg
    13501 tcaagagttc cgtcgaagaa accctgccac ggcggggagg caaggcacgc cccctctcca
    13561 aggaggactt ctcccaaaag agcgcaattt tcctccagcc aaaggcacag ttcgatcatc
    13621 ttgtagctct cagtgacagc agcgaccgcg caagggccat catcgaagcc atggaatcga
    13681 tagagagcga ttacgaaaac ctgcggggcg tgctccccaa aagcgaatat caggaactgg
    13741 acaacgatgt tctcggtcaa ctccttcgta ctctgaatcc tgatgaactc aagaaggtca
    13801 atggcgatgt tttcggccgt atctacgaat actttctgac ccagttcgct gatcttaaag
    13861 cccatgatgg aggtgagttc ttcaccccgg tttcgctggt ttcgctcatc gccaacgttc
    13921 tggaaccaga aaatggcgtt gttctggacc ctgcctgtgg ttcgggagga atgttcgtcc
    13981 agagcgcccg atttgtggaa cgaaggcggg aaaaccctgt ggacaggctc accttttacg
    14041 ggctggagaa gaatgccacc acgatacgcc ttgcaaagat gaacctggct gttcacggcc
    14101 ttgagggcga tatccagaag gccatcacct actacgaaga cccccatgaa ctgctgggca
    14161 aggcaaactt tgtcatggcc aatccgccgt tcaacgtcga cgagatcgac gccgacaaag
    14221 tcaggaccga cccgcgcctg cccttcgggt tgcccggtgt caacaaggga ggcaaggttt
    14281 caaacggcaa ctatgtctgg ataagttact tctacagcta cctcaacgaa aatgggcggg
    14341 cagggtttgt gatgtcctcc caggcttcca gtgctggagg gggcgaggcg aaggttcggc
    14401 agaagctcgt cgagaccgga gatatggacg ttatgatcgc catccgcccc aacttcttct
    14461 acacacggac agtgccttgc gagctatggt tcattgatcg gggcaagccc gatcaaccct
    14521 cacccctggc ccctctccca gagggagagg aggggaactc ttatcattat cgtggtggtt
    14581 ataacttcag cggcttggtg gagttggcgc gagagtttag aaaaaatcag acgcctgccg
    14641 aggattgttt ctggagcatt gtacagaaca ggaaatttct gggccttaag ttccgcagac
    14701 aacaccaggt gggggattat gttgtcgatt tctactgcaa ggaagcatcg ttggcggtag
    14761 aacttgatgg ccccgttcac gatttacaga caaagaggga cgccaaacga gatgcatatc
    14821 taaaatccca gggtgttcat gtccttcgga tcaaaaacgg agacctgctt gaaaaaacgc
    14881 aggcgacgct ggagcagttg gccaatcgca ttttttcttc ttctgaaatt cctctccctc
    14941 tgggagagga aacaggtgag ggggacgttg cccttaatca caagggcaac gtcctgatga
    15001 tcgacgcgcg aaacgtctac cgcaaggtca cccgcaagat ctacgatttt tcgcccgaac
    15061 aggaacaaaa catccttgca atcgtctggc tctaccgggg tcaaaccgaa aaatatctcg
    15121 atcttgtagg cggctattgc cgccgaatgt tggccgaagg cgaagggtgt ttcgccgccc
    15181 gggatgacag cggcgaaaag acaaaacctc tgccgtcgtt tatcgcctct cttaccgctc
    15241 tgcgggaagc gttacaggca tttttggcga acccatccaa ggatgttgct tttgaggaac
    15301 ttcaaaagga actcgaagaa gccgtgtccg ttttcaaggg tgacgttgaa actttccgtg
    15361 aacatctttc cgaacagcaa tcaacctggg aaggacagaa aatcacaaat ggcgaactta
    15421 aaaaggccgt agaccgattg gtgtcactgg gcgaatcaag ccgggatctg gtcaagcaga
    15481 cggatcttat cttcaaaatt gcctcccgct ttatggaagc aattccacag gcattcgcac
    15541 cccgacaatc ttccggtgga aacggcatcg gtcagatgaa gaggcagctt cgagacctgg
    15601 atgttgcccg cgctctggcg gtcgatcagc tcaagcaggt tcgctacttc tggaaacagg
    15661 cccgctggct gacggagcgc ttccccgaag caaaactgtg cgacgtgccg ggtctcgtca
    15721 aactcgtgga caaagccgag atcgaagcca acgactggag cctcaccccc ggccgttacg
    15781 tcggcgtcgc ccccgaggaa gtggacgaga atttcgactt cgaggaaacc ctgcgcgaaa
    15841 tccatgtgga act
//
LOCUS       DAOTIQ010000045        15689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4521_length_15689_cov_7.402072, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000045 DAOTIQ010000000
VERSION     DAOTIQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 15689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15689
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_15689_cov_7.402072"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..781
                     /locus_tag="P5541_06640"
     CDS             26..781
                     /locus_tag="P5541_06640"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HRV71697.1"
                     /translation="MIFPFQAFEIASLEELAEGVMEDPETKAKICLKCGRTFETGEVF
                     EVDGRFWEAEAAARIHSESEHGPQFGYLLDLLKQWRGVSDPQARLLEAFFKGDDDKKI
                     SSELGISRSTVRNHRFRMKERSRQAKVFLAIMKILEERQKPEARLVDFPANARMSDER
                     YAITREEYEKILRKYFRDDGTLVRFPLKARERVALLRKIAGLLRPGERYSERELNGIL
                     SEVTEDHVLVRRCLVDYGFVSRMRDGSAYWIEL"
     gene            complement(904..2370)
                     /locus_tag="P5541_06645"
     CDS             complement(904..2370)
                     /locus_tag="P5541_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV71698.1"
                     /translation="MTNRRPGSVLLFVLVLSGIVFSLFEPAEAQVDAAALSRAMKSIA
                     VLTAETKDGKESRGLAFLMAGEGRAVTAAHLLKNASRVTLRFQDGAESLSPGIIAVDE
                     KRGLAMIEVPASGRQGLTLVQTSISPGTMVHCGAVRDGTYGFVQLSVSEIHQGASGVE
                     RYALSGEGPSGNSGSPVLDQKGNVTGMVIETTEGRLLVPSVFIAAIDASSPLKSWGGE
                     IPEMVDDPQADPGPGPMDEIDQAILDFFVILYDHDSVYRWGQETTNGKGYLQGVPEDV
                     YHYQTKLEMAIKKLVGADTNDPLRKKIIMNLKEVGNNQVAAVNYFIQAVVTGQGAKDW
                     GAQSQDLNKRARAAMNIAGEVLLSELPAIHELYERSTVLGEKMHRDIKYYLGIEKRVA
                     SFAIGAVTTIEKPFRLIVLYSETFGEALGLRSGDTILSAAGSRFDSEGSMEDFKAVIL
                     QNLGKTIDVVVERNGEQTTLKMKIPEEIPEKYVLPQIF"
     gene            2522..3193
                     /locus_tag="P5541_06650"
     CDS             2522..3193
                     /locus_tag="P5541_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71699.1"
                     /translation="MITASENWRASFPETSFGIMVIEGILNSGDNPGFEDRKALLEEE
                     LRMRHRGKTRKQIGKEPPFEAYEKFYRTFGKGYPVLHQVETVALKGEPVLCPSPLVTA
                     MFMAELKNGLLTAGHDLDKVSIPLSLDISKGTEAYQSMGGKERNLLKGDMFLSDQRGI
                     LSSVIYGPNNSSSMTAKTTRALFTVYGVPSVSSMQLRAHLEEIDEFVRLFSPQAVRRE
                     LFMLD"
     gene            complement(3251..3955)
                     /locus_tag="P5541_06655"
     CDS             complement(3251..3955)
                     /locus_tag="P5541_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV71700.1"
                     /translation="MRKFVIDKKVFVLFPLARVCGLIVEELDNSITWDGKEMLEQACR
                     EAALRLGEVPFAESPFFAPWRAAFRTFGAPKGYRSSVEALAKRAVKGSCPPSVNSLVD
                     LYNVTSLKFLFPCGAEDLDAVVGDIRLTAAQGGEPFLPLGAEEDDPPREGEIAYLDDV
                     GAICRCWNWREADRTKLTPRTKRAMLFMENLAPERDPELREALAYIGQAVRERLGAKT
                     RSFWLDGGCMEVDLPE"
     gene            complement(4028..4999)
                     /locus_tag="P5541_06660"
     CDS             complement(4028..4999)
                     /locus_tag="P5541_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV71701.1"
                     /translation="MPGFLRRRQFKWILGALLFAMALLGGLFCIDMYRAYGRIKGESR
                     ILASPYGDIEYVEGGDGPDVLVIHGSGGGYDQGKIIVRSVLGDDFHWVVPSRFGYLGS
                     TFHEGATFEEQARAYGFLLDHIGIGQAAVVALSHGGPSALLFAAMYPERVSSLTLISC
                     GVASSSTGGQVQADRKGDLLVTIYRHDLLYWSLTRFFRKQFIGLMGASGEVFAGLSPE
                     QRELVDQVIYYMNPSSQRSAGAAFDNSADMPNERIKAIQSPTLVFHALDDMLQLFHNA
                     EYAEANIPLARLVSFDKGGHLLMIVEQERIREEIKRHILESSRSNNS"
     gene            5285..7009
                     /gene="ade"
                     /locus_tag="P5541_06665"
     CDS             5285..7009
                     /gene="ade"
                     /locus_tag="P5541_06665"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRV71702.1"
                     /translation="MHKFSLLAASRGDIPADLVLKGARIANVLSMDYEDADVAIHEGR
                     IAGIGKGYEGKENLDLSGMILAPGFFDAHCHIESTMLVPSSFAGLVAARGTSAVAADP
                     HEIANTSGMAGIEYMFHDSEYAPVDIFYTAPSCVPASSFETPLEYLGADEVAEMFSRG
                     WCHSLGEVMNFPAVISGENDIWDKIRVSSGRIKNGHAPGLAGRDLCAYLVSGCDSDHE
                     CVLASEALEKLRRGMWLMIREGATEKNLEELAAIVRDDEKRACRCMLVSDDLTAAYIR
                     DRGHMDEKVRLAIAFGISPMAALRMVTLSPANYFGFRDRGAIAPGYIADIVAVEDLES
                     CKVRRVWKEGRLVSSDGVLNMPCKKAEITDAVRVSSSSLTVPRTEDLAVPIREGQKIR
                     VIGVIPGQVTTEHRILEPRIISGKATADPSRDIALLAVMEKNRGTGRLSLGFVQGLGL
                     EEGALGSSVAHDAHNYVVAGMDELSIRTAFEKLVSAGGGLVAARGNQVKAFLSLPVGG
                     LMSPDDPEEIIEGLKVVEKAAADLGSTIENPFMTMSFLCLSVIPELKLTDSGYVDLSR
                     GGAQGVTA"
     gene            7410..8390
                     /locus_tag="P5541_06670"
     CDS             7410..8390
                     /locus_tag="P5541_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV71703.1"
                     /translation="MRRNLLITSVLLFILVFCTQVVAQEHKLIFGHMGTTDTAYHAVA
                     EFVAERVTELTNGNVKIEVYPNAELGDEQDLFEQVMMGVTDFTIVNPGVTVEFSQTMN
                     FFNFPFLYSSREHWERVFTSPLVQKLSERVEKEAGVMILGVIGGGERFVVSRKPIQKV
                     EDLDGFLMRLAPASITIDTWSSLGVQPTVVAYNEIYSALQMGVIDGLENEPEWILRMR
                     FYEQAPYLVRTAHEIVSRPIVMSVDSFKGLPKEYQDAVLTAAKEGAELGRELGIKLDK
                     ESLDELVNKYDVRVFDVDIDKIRGKTAGVIEKNAKQLQLYDLVEEVSELR"
     gene            9094..10404
                     /locus_tag="P5541_06675"
     CDS             9094..10404
                     /locus_tag="P5541_06675"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV71704.1"
                     /translation="MFDVILQPQALILILFVVFGFLAIIGVPIAFALGISSLSVILLD
                     PRLTIWPFFQRSFYAIDSFVLLAVPLFMFTGTLMNSSRFTDKLIEFSRCIVGPVRGGL
                     AHVNIIASMLFAGISGSTNADTAGIGSILIPAMKKEGYPISISTAVTAASSVMGVIIP
                     PSLLMVVWSSITETSVAGLFLGGVIPGILIGFSQMGIIAVLAKKDNYPRTSFPGFKKL
                     LASSKHGLIALGAPVLIIGGILGGFFTPTEAAVAGIIYCLFFSVVFYRQLTIAGFIEA
                     CWETARITSLALFCVAMASIFGWLISYYRLPRILMEYVTIENPIFLLMFTTVVMLLFG
                     TFMDALPAMAILGPLFLPIIRQAGIHPVHFGVVSVVALALGFITPPYGLSLLISCKIA
                     GIPITKAFKDTMLFFGVALVLIILMILFPQIVLGLPQLIAPRFM"
     gene            10625..12652
                     /locus_tag="P5541_06680"
     CDS             10625..12652
                     /locus_tag="P5541_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV71705.1"
                     /translation="MKELKRETLFINNNEFLFYRCHTLLVGSGAAALNCACHLEDFGI
                     HDLVIATEHLGGGTSNNSGSDKQTYYKLSLFGEECDSVYEMARTLFSGGAMHGDIALI
                     EATMSTPEFFHLVQGGVPFPHNCFGGYVGYKTDHDPKQRATSAGPWTSSQMFKALLAQ
                     VREKGIPILDRHEVIALLTTGQGEEKRTIGALAIDKTKVHLGLPSIVIFQAENIVFGT
                     GGPGGLYKNSVYPNGHLGSTGVALEAGAKGVNLTEWQYGLASIQFRWNVSGTFQQVIP
                     RYFSTEPDGSDEREFLKRHFPSMRQLATAIFLKGYQWPFDPRKIKDHGSSLIDILVHE
                     ETVGKGRRVFMDYRRNLSPEPGFGVFSLDDLETEAYTYLKKSHALLPTPIARLEQMNP
                     MAIQLFKDNGIDLTCEPLEVAVCSQHHNGGLRGNIWWESNLRHFFPVGEVNGTHGIYR
                     PGGSALNSGQVGGYRAADFIAHRYQSISFCKEEFLDLAKAQIEGTLELAKRALNCVGR
                     KGQTISAFRQEFQERLSRAAAHIRALGPIRNAIQEAESQFARIESGNISLESNKGLVD
                     FFQNRQLCLTQLAVLVSILSYLERGGGSRGSYLVLDEDGEPILEKLGFEWKFKPELKE
                     LRDYVQVYRYEKGEHLIEWETVRPIPEDNYWFENVWQSHLGKDIYQDEKGE"
     gene            12843..13613
                     /locus_tag="P5541_06685"
     CDS             12843..13613
                     /locus_tag="P5541_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV71706.1"
                     /translation="MSEQRSKTLQLLERTFAILDYISLAEKPCMLTEIVEKTGISKPS
                     VHRILSTLHHYRIVVKDRHNQYRMGPKIIQWGRACKGDSNLLSIASPYIEKIWNITGE
                     TIHLVSFEDGYAYYLMKKESRYPLQMRSRRGDTILLHSTAAGKAILFSLPFDELLSFL
                     KRTPLDKRTENTITDPKIFIKQREEFMSKGFTEEEEENERDIRCIGVPILNDKGDPLG
                     AVSITCPIFLCDDKRSEELGELLKREASKISQEFGYSD"
     gene            13856..14884
                     /locus_tag="P5541_06690"
     CDS             13856..14884
                     /locus_tag="P5541_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRV71707.1"
                     /translation="MAKIVTFGEIMLRLSPPERELLLQTPQFVATFGGAEANVAVSLA
                     NYGEDVSFVTALPENPIADAAIAELRKLKVGTDDILRTGERIGIYFAQAGSVMRPSKV
                     VYDRSHSSISQVERGDFDWDRILQEATWFHVTGITPAIAEGTAEVTLEALGKCKEKGI
                     NISCDLNYRKKLWKWGKPSVEVMAEVTKFVDVLIANEEDCQKCLGIEVDVDVTSGSLD
                     VDKYRNLASRVMETYTGIKYLAVSLRESISADHNNWSGVLATRDGFHQSRKFNITHIV
                     DRIGGGDAFGAGLIWGLNHLENEQVALDFAVAASAIKQTIYGDFNRITLNEVLNLMKG
                     DASGRVQR"
     gene            14938..15594
                     /locus_tag="P5541_06695"
     CDS             14938..15594
                     /locus_tag="P5541_06695"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HRV71708.1"
                     /translation="MGKTEKFEILGRIHSKGIVAVIRAESADSGRKIADAVFEGGIPA
                     IELTMTVPGALSIIETMAEYYKGRDIILGAGTVMDPETARLCILGGAQYVVSPILNPE
                     VVKMCNRYGIPAMPGIGTVTELVKAMELGVDVVKCFPGEVLGPAFIKAVHGPVPEAKI
                     MPTGGVSLSNIQKWLEAGAFALGMGSALTKPDGRKDDYQAITRTAQKVIRQIVDFRKT
                     "
BASE COUNT         3981 a         3738 c         4063 g         3907 t
ORIGIN      
        1 aaacaagcag gagggggttt tgtttatgat ttttcctttt caggcttttg agatcgcatc
       61 cctggaagag cttgcggagg gggtgatgga ggaccctgaa acaaaggcaa aaatatgtct
      121 taaatgcgga aggaccttcg agacaggaga ggttttcgag gtggatggaa gattttggga
      181 ggccgaagcc gcggcgcgga tccactccga gagcgaacac ggaccccagt tcggttacct
      241 gttggacctg ttgaagcagt ggaggggtgt ctcggatcca caggccagac ttctcgaagc
      301 ctttttcaag ggtgatgacg acaagaagat ctcttcggaa ctcgggataa gccgctccac
      361 agtcaggaat catcgcttca ggatgaagga aagatcaagg caggccaagg tcttcctggc
      421 gataatgaag atcctcgaag agcgccagaa gccggaggca aggctggtgg atttccctgc
      481 caacgcaaga atgtctgacg aacggtacgc catcacccgg gaggagtatg aaaagatcct
      541 caggaagtac tttcgggacg acgggaccct ggtccgattc cctcttaaag ccagggagcg
      601 ggtggctctc ctgagaaaga tagcggggct tttgaggcca ggtgagaggt actctgaaag
      661 ggaactgaac gggatccttt cagaggtcac tgaagatcac gtacttgtca ggagatgcct
      721 ggtggattac ggtttcgtca gtcgaatgcg tgacgggagc gcctactgga tcgagctctg
      781 atccgggttt aaaaccacct gacgcaactt ctcaaaaccc caggggaaac agaagggggc
      841 aaaaaagagc tcaaaggcag accctcctct tttttggccc ccatttattt atggtctgaa
      901 aacttaaaag atctgcggaa gcacatattt ttcagggatc tcctccggga tcttcatctt
      961 aagggtcgtc tgttccccgt ttctctccac caccacgtca atggtcttcc caaggttttg
     1021 gaggatcaca gccttgaagt cctccatgga cccctcggaa tcgaatcttg acccggccgc
     1081 ggaaaggata gtatcaccag atctcaggcc cagcgcttcc ccaaatgttt cgctgtataa
     1141 cacgatcagg cggaagggtt tttcaatggt cgtcacggct cctatcgcaa aggaggccac
     1201 ccgtttctca atgccgaggt agtacttgat gtccctgtgc atcttttcgc ctaacactgt
     1261 cgatcgttcg taaagttcat gtatcgccgg gagctccgac agtagcactt ccccggctat
     1321 attcatggca gccctggccc gtttgttcag atcctgggac tgggctcccc aatcctttgc
     1381 cccttgccct gtcactacgg cctgaataaa gtaattcact gcggccacct ggttgttccc
     1441 tacttccttg aggttcatta ttatcttttt tcgcagaggg tcattggtat ctgctccgac
     1501 cagttttttt atggccatct cgagcttggt ctgataatgg taaacatcct ccggtactcc
     1561 ttgcagataa cctttcccat tggtcgtctc ttgcccccac ctgtaaacac tgtcgtggtc
     1621 atagagaatg acaaagaaat cgagaatggc ctggtctatc tcgtccattg ggcctggacc
     1681 tggatctgcc tggggatcat cgaccatctc agggatttca ccgccccagg acttgagagg
     1741 agaagatgcg tcgatggcag caatgaagac cgagggaaca agaagccttc cttcagttgt
     1801 ctcgataacc atgcctgtta cgttcccctt ctgatccagg acaggtgagc cgctgttccc
     1861 ggaaggaccc tctccggaga gggcgtacct ttccacaccc gaggcaccct ggtggatctc
     1921 ggagaccgac agctgtacga acccgtaagt tccgtctctt accgcgccac agtgtaccat
     1981 cgtcccaggg ctgatgctgg tctgcaccag tgtgaggccc tgccttccgg aggcaggtac
     2041 ttcgatcatc gccaggcctc ttttttcgtc aaccgcaata atgcccggag aaagggactc
     2101 agccccatcc tggaaacgga gcgtgaccct ggaagcgttc ttgagaaggt gagccgccgt
     2161 cacagccctt ccttctccag ccattagaaa tgccaggcct ctgctttcct ttccgtcttt
     2221 cgtttcagcg gtgaggacgg caattgattt catcgccctg gaaagggctg cggcatccac
     2281 ctgggcctcc gccggctcaa acagcgaaaa gactatccct gacagcacga ggacaaaaag
     2341 caaaacagaa cctgggcgcc tgttagtcat cgctccaacc actccagtct tcccggatcc
     2401 tgagaccgga ccgggcaaaa tacgctaccg agtaaatctt acatcaacgg ggcgtggaag
     2461 gttctttgtt tgcctatatt atatattagg ctagaacgag aagaagggag ggaccagtcc
     2521 gatgatcact gcttctgaga attggagggc gagtttcccg gaaacctctt tcgggatcat
     2581 ggtgatcgag ggcatcctga actcgggaga caaccccggg ttcgaagacc ggaaggcctt
     2641 gcttgaagaa gagctccgca tgcgacatcg tggcaagacc aggaagcaga tagggaagga
     2701 accgcccttc gaagcctacg aaaagttcta ccgaacattc ggaaaaggtt atccggtgct
     2761 tcaccaggtc gaaaccgtgg cactgaaagg ggaacctgtc ctctgcccct cacctcttgt
     2821 aacggcaatg ttcatggcag agttgaagaa cggccttcta acagccggcc acgatctgga
     2881 caaagtatcc atcccccttt ccctggatat ttcgaagggg acagaggcct atcagtctat
     2941 gggggggaag gaacggaacc tcctgaaggg cgacatgttc ctctccgacc agaggggcat
     3001 tctttccagc gtcatttacg gacccaataa cagcagttca atgactgcaa aaacgacccg
     3061 tgcgctattt accgtctatg gagtgccgtc ggtctcctcg atgcagttgc gggctcacct
     3121 ggaagagata gatgaattcg ttcgcttgtt cagcccccag gcggtccgca gggaactttt
     3181 catgcttgat tgaccagcgg atcgattctg gtttcctgga tctgcctctg ggacctgatc
     3241 ctctcttggt tcactccgga agatccactt ccatgcatcc gccgtcaagc cagaaactcc
     3301 tggtctttgc gccgagtctc tcccggaccg cctggccaat ataggccaac gcttcccgta
     3361 gctctgggtc ccgttctggc gcaaggtttt ccataaacag catggcacgc ttcgtccggg
     3421 gggtcagctt ggtcctgtcg gcttcgcgcc agttccagca tcggcagata gccccaacgt
     3481 catccagata agctatctct ccctcgcgag ggggatcgtc ctcctccgcc ccaaggggga
     3541 ggaacggttc accaccctga gcggcggtca agcggatgtc gccaaccacc gcatcaagat
     3601 cctccgcacc gcaagggaag aggaacttca gcgacgtcac gttgtacagg tccaccaggg
     3661 aattgaccga tggcggacag ctccccttca cggcgcgttt tgcaagggct tccacggaag
     3721 aacggtaacc cttgggggct ccaaaggtcc ggaatgccgc cctccagggg gcaaagaaag
     3781 ggctctccgc aaaaggaacc tctcccagcc taagggccgc ttcccggcag gcctgctcga
     3841 gcatctcttt cccgtcccag gttatgctat tgtccagttc ctcaacgatc agcccgcaaa
     3901 cccgggccag agggaaaaga acaaagacct ttttgtctat cacgaatttt cgcaatatcg
     3961 ttcatcctct cacagatccg gatacttcgt atcttaccag tcgaagtgct tgaattggat
     4021 acaagtccta tgagttattt gatcttgagc tttccagaat atgcctcttt atctcctcac
     4081 ggatcctctc ctgctccacg atcataagaa ggtgcccgcc cttgtcgaaa ctgacgagcc
     4141 tggccaaggg gatattagct tcggcgtatt cggcgttgtg aaagagctga agcatatcgt
     4201 ccaaggcgtg aaacacaaga gtgggggact gtatggcttt tatcctctca tttggcatat
     4261 ccgcactgtt gtcaaaggcc gcaccagcgg acctctgcga agaagggttc atgtaatata
     4321 ttacctggtc gaccagctct ctctgttcgg gtgaaagacc ggcaaaaacc tccccgcttg
     4381 cccccatcag cccgatgaac tgcttcctga aaaaccttgt caggctccag tagagaagat
     4441 cgtgcctgta gatagtgacc agcagatccc cctttctgtc cgcctggacc tggccacccg
     4501 ttgacgaaga tgccacaccg caggagatca gggtcaagga ggagacacgt tcggggtaca
     4561 tggccgcgaa aagcagggcc gaaggacctc catgggacag agctacaaca gcggcctgac
     4621 cgataccaat atgatccaga agaaatccat aagccctggc ctgctcttcg aaggtggcac
     4681 cttcatggaa tgtggatcca aggtatccga aacgggaagg tacaacccag tggaagtcat
     4741 ccccaaggac agaacggaca atgatcttgc cctggtcgta tccgcccccg ctcccatgga
     4801 tgaccagaac gtcagggcca tctccaccct cgacgtactc aatatccccg taaggagaag
     4861 cgaggatccg gctctcaccc ttgatccgtc cgtaggcccg atacatatcg atacagaaga
     4921 gccctcccaa aagggccatg gcaaaaagca gggcccctag gatccattta aactgccttc
     4981 tccgaagaaa acctggcatc ctgctgatca cctccgcagg gcaatctgcg ggtaacatcg
     5041 atatatggag gattgtagac ccaaacaagc tgatacggac caaagtgcca gatcttcatc
     5101 aataacaatg gccaggcctg ctggtacata acacataaaa cggggatcgg acaggaaacc
     5161 ttttccttcc cccttttttg cagtgctaga ataaggaaga gctcttcccc ttaaaatacg
     5221 ggtgttcaat aacagcaaac agaaagaatt caccggtcag agatcattcc aggagatgat
     5281 attcatgcat aagttttccc tgcttgcggc gtcaagaggc gatatccctg ctgatcttgt
     5341 cctgaaaggg gcacgaatag caaacgtcct ttcaatggat tacgaggatg ccgacgtggc
     5401 gatccacgaa gggaggatcg ccggaatcgg taaaggttac gaggggaaag aaaaccttga
     5461 tctttcgggg atgatcctcg ctccgggctt ttttgatgcc cactgtcaca tcgagagcac
     5521 catgctcgta ccatcatctt tcgccgggtt ggtggcagcg agaggaactt ccgccgttgc
     5581 cgccgaccct cacgaaatag caaacacctc cggcatggcc gggatcgaat acatgttcca
     5641 tgacagtgaa tatgcccctg tggacatttt ctacactgct ccttcgtgtg tgccggcctc
     5701 gtccttcgag acccccctgg agtatcttgg cgccgacgag gtggccgaga tgttctcaag
     5761 gggctggtgc cattctctgg gggaagtaat gaacttcccg gccgtaatta gcggggaaaa
     5821 tgatatctgg gacaagatcc gggtttcctc tgggaggatc aagaacggcc acgctccggg
     5881 acttgccggc agggacctgt gcgcctatct tgtcagcggc tgcgactcgg accacgaatg
     5941 cgtccttgcc agcgaagctc tcgagaaact caggagggga atgtggctga tgatccgtga
     6001 gggggccacc gaaaagaacc tggaagagct ggcagcgatc gtcagggatg acgaaaaaag
     6061 ggcatgtcgc tgcatgcttg taagcgacga cctgaccgcg gcctacatcc gcgacagggg
     6121 acacatggac gagaaggtca ggctggccat tgccttcgga atatccccca tggcagccct
     6181 gcggatggta accctttctc ctgcgaacta tttcggtttt cgagaccgtg gcgccatcgc
     6241 tccggggtac atcgccgaca tcgtcgccgt ggaagacctg gaatcgtgca aagtcaggcg
     6301 ggtatggaaa gagggcaggc ttgtatcctc cgacggggtc ttgaacatgc cctgcaaaaa
     6361 ggccgagatc acagatgccg tgagagtttc aagttccagc ctcactgttc ctcgtaccga
     6421 agacctggcg gtcccgatcc gcgaaggcca gaagatcagg gtgatcggcg tcattccggg
     6481 acaggtaact acagagcaca ggatactgga gccaaggatc atctccggaa aagccacagc
     6541 agacccttca cgggacatcg cccttcttgc agtaatggaa aagaacaggg gaactggaag
     6601 gctttccctt ggcttcgttc agggactcgg ccttgaagag ggcgcccttg gctcctccgt
     6661 cgcccatgat gcccataatt acgtcgttgc gggaatggac gagttgtcca taaggaccgc
     6721 cttcgaaaaa ctggtctcag caggtggggg acttgtagcc gcaaggggta accaggtgaa
     6781 agcattcctt tctctgccag ttgggggttt aatgagcccg gatgaccccg aagagatcat
     6841 tgaaggcctg aaggttgtag agaaggctgc ggccgacctt ggatctacca tcgaaaaccc
     6901 ctttatgacg atgagcttcc tgtgcctttc ggtaattccc gaactcaaac tgaccgattc
     6961 gggttacgtc gatctctccc ggggaggagc ccagggggtt acagcctgaa atcaacggat
     7021 caaaaacctt ccgggagcct ttgaatcaag cttcaaacaa aaaggggcca gatctgctct
     7081 tttgtaaaag agcaggcttt cctcccagga cttgttccta ctccagaact atggttcttc
     7141 tacaatcctc agaagaaaaa tgcggcctaa cccaatcgga tcctgccggt tttctcagtt
     7201 aatgaacaca ttcctttaca gcgatcaaca ttttgacaaa tattcgaaga tagtagatac
     7261 taaaacagat atccggtaaa cggaatatag gtccgcctgg cggacttttt gtcaggatgt
     7321 tacccaaatt ccagtcagat tgaggaaagg gggttgtaga ctaaggaatc tggttcaaaa
     7381 aggagctctc tcagagagga ggaaatcaaa tgagaaggaa tttattaata acatctgtgt
     7441 tgctttttat tctagttttc tgtactcaag tcgtagctca agaacataaa ttaatattcg
     7501 gacatatggg gaccaccgat actgcatatc atgctgttgc agaattcgtt gcggaaaggg
     7561 tgacggagtt aaccaacggc aatgtgaaga tcgaagtata tccaaatgct gaattgggcg
     7621 atgagcagga tcttttcgaa caggtaatga tgggtgtaac agatttcact atagtgaacc
     7681 ccggagtgac tgtcgagttc tctcagacaa tgaatttttt caatttcccc ttcctgtata
     7741 gcagccgtga gcattgggaa agggttttta cctcacctct agtacaaaaa ttgagcgagc
     7801 gggtggaaaa agaggctggg gtaatgatcc tgggggttat tgggggagga gagaggtttg
     7861 ttgtcagccg gaaaccgatc caaaaagttg aagatcttga tggtttctta atgaggctgg
     7921 cacctgcaag cattaccatt gatacctggt cgagcttagg ggtccagcca acggttgtcg
     7981 cttacaatga aatttattcc gctcttcaaa tgggtgttat cgacggtctg gaaaacgagc
     8041 cggaatggat cttgcggatg agattttacg aacaggcccc ttatctggtt cggacagctc
     8101 acgagatagt ttcacgaccg attgtaatga gcgtggattc tttcaaaggt ttaccaaaag
     8161 agtatcagga tgccgtctta actgcggcga aagagggagc cgaactcggc agagaactgg
     8221 ggattaaact tgataaggaa tctttggacg aactagttaa taaatatgat gtccgcgttt
     8281 tcgatgtgga tattgacaag atcaggggaa aaacagcagg tgtgattgaa aaaaatgcca
     8341 aacaacttca attgtacgat cttgtggagg aagtatcaga actgcgttaa gcgaccaagc
     8401 aaaacatgtg gctgtccttt cttccacagg taagacgaaa ggatagcctg aagaactcga
     8461 ttggagactg gggaatggtc tttgaatgaa tcggtaaata tatttgagga ggccggggaa
     8521 ctggcctcca aatacttttg aggtggagaa ttagtggaca agattacccg attttgtcta
     8581 ttctacgcaa caattctaaa aaaaatcgtc tctggtttgg tccccgtatt gatcctgacc
     8641 gtgacatatc agattgttgc tcgtcatatc tcctatatac cacgtttttt gtggaccgag
     8701 gagatatgtc gcttcacgct taattggctt gttatgcttg gctcggcatt ggctgttcga
     8761 aatagtgacc actttgatat cgacatcctc tccaatctga caccccgggg cagagaaatc
     8821 cagttttggg ttgtaaattc actaattatt ctatctgctc ttctttttac agtttttggt
     8881 tgggggtttg ctgtttctgg tttacggcgt cgttcattgg ccgcaggatt atcaatggtt
     8941 tgggtttaca gttcatttat ggtttttggg gtatcgtctc ttctatttca gttagaaaaa
     9001 gttgtccgac atttcaataa gagcgagact ttatcacatt aaggttttcg cgaggcgttt
     9061 ttaaatgacc tttggccgag ggagataatt attatgttcg atgtaatcct tcaaccacaa
     9121 gcattgattc ttattctttt cgtagtcttt ggttttctgg ccataattgg agttccgatt
     9181 gcatttgctt tgggcatttc gtccctctcg gtaattcttc tggacccgcg attgaccata
     9241 tggccctttt tccaacggag tttttatgcc attgattcct ttgttttgtt ggccgtgcca
     9301 ttgtttatgt ttacagggac attaatgaat agctctcgtt tcacggataa actcattgaa
     9361 ttttcccgtt gtatcgttgg accagtcaga gggggattag ctcatgtcaa tataattgcc
     9421 agtatgcttt ttgccggtat ttcagggtcc actaatgctg atactgccgg aattggttca
     9481 atattgatcc cggccatgaa aaaggaggga tatccgatct ccatatcgac tgccgtaacc
     9541 gctgcttcgt cggttatggg agtcattatc ccccctagtc ttctgatggt agtgtggtcc
     9601 tcgataaccg agacctccgt tgccggcctt tttttgggcg gagtaattcc tggaattctt
     9661 atcggtttca gtcagatggg gataatagca gtactggcta aaaaagataa ttatccccgc
     9721 acctctttcc caggatttaa gaagctatta gcctcaagca aacacggtct gatcgcttta
     9781 ggagccccgg tcctgataat cggaggaatt cttggaggct tttttacacc aactgaagcc
     9841 gcagtggcag gaattattta ctgtctgttc ttttctgttg tgttttatcg tcagttgacc
     9901 attgcgggct ttattgaagc ttgttgggaa accgcgagaa ttacgtccct ggccctattt
     9961 tgtgtagcaa tggcctcaat tttcgggtgg ctaatttctt attatcgtct tccaagaata
    10021 cttatggagt atgtaaccat agaaaacccg atattccttt tgatgtttac cactgttgtg
    10081 atgttactct ttggaacctt tatggatgct ttgcctgcta tggcaatact gggcccgttg
    10141 tttttgccga ttattcggca ggcggggatt cacccggtac attttggagt tgtttccgtt
    10201 gttgctctgg ctttgggctt tatcactcct ccttacggtc tttcgttgct aatttcatgc
    10261 aaaattgccg gtattccgat tacaaaggcc ttcaaagata ccatgttatt ctttggggta
    10321 gctctggtac tcattatttt aatgatcctt tttccccaaa ttgtattagg tctgccccag
    10381 ctgatcgcac ctagatttat gtagctgtca aacctgtttc ccttcttatc accaagacag
    10441 aaccagctat cgcggtttgg gaagatctgg gctcttcgct gaaataaatt aagggccgat
    10501 tccggggaaa atgcatcttt tttaaattcg tgccatttct ggattttcct ttaagtggag
    10561 atagatctta cattgggaat accggcaaga gatggaacgc ctgttcaatt aggaagataa
    10621 gggagtgaaa gaactgaaac gggagacgct gttcataaac aataacgagt ttctttttta
    10681 ccgttgccat acactccttg tcggttcagg cgccgcagca ttgaattgcg cttgccattt
    10741 ggaggacttc ggaattcacg atctggtaat tgctactgaa catttaggcg gtggcacttc
    10801 aaataactcc ggctcagata agcagaccta ttacaaactc tctttattcg gtgaagagtg
    10861 tgattccgtt tatgaaatgg cccgtacatt gttttccggc ggagcaatgc atggtgacat
    10921 tgctttaatt gaagcgacaa tgtccacccc tgaatttttc cacctggtcc aaggaggggt
    10981 tcctttcccg cataattgtt ttgggggata tgtgggttat aaaaccgacc acgatcccaa
    11041 gcaaagggca acatctgccg gaccatggac ttcatcccag atgttcaagg ctctgttagc
    11101 acaagtacga gagaaaggca ttcccattct tgaccgccat gaagtaatcg ctttgctcac
    11161 cactggacag ggggaagaga aaagaaccat tggcgccctt gccatcgata aaaccaaagt
    11221 ccacctcggt ttaccgtcga tagtgatctt tcaggccgaa aatattgtct ttggaacagg
    11281 cggtccggga ggtttataca aaaattctgt ttaccctaat gggcatctgg gtagtaccgg
    11341 cgtcgccttg gaagcaggag cgaaaggggt taatttaacg gaatggcagt acggactggc
    11401 atcgatccaa tttcgctgga atgtttcggg tactttccag caagttatac ccaggtattt
    11461 cagtacagaa cctgacgggt ccgacgaaag ggagttcttg aagcggcatt ttccttcaat
    11521 gcgccaattg gcgacggcca tcttcctaaa aggttatcag tggccctttg atccgaggaa
    11581 gatcaaagat catggctctt ctctgatcga cattcttgtg catgaggaga ctgttggaaa
    11641 aggaaggcgg gtatttatgg attaccggcg taatctttcg ccggaaccag gttttggcgt
    11701 cttttcactt gatgaccttg aaacagaggc ttatacctat ttaaaaaaat cccatgcgct
    11761 cttaccaact cctatagcca ggttggaaca gatgaatccc atggcaatcc agttatttaa
    11821 agataatggc attgacctca catgcgaacc cttggaagtt gcagtgtgtt cccagcatca
    11881 caacggcggc ctgagaggga atatttggtg ggaatcaaat ctccgacact ttttcccagt
    11941 gggtgaggtc aacggaacgc atggcattta ccgacccggc ggatcagcac tgaattccgg
    12001 acaagtggga ggatacaggg cagcggattt tattgcccac agatatcaga gcatcagttt
    12061 ttgcaaggaa gaatttcttg atctggcgaa agctcaaatt gagggaacac tggaattagc
    12121 caaacgggct ttgaattgtg tcggtcggaa ggggcaaaca atttcagctt ttcgccagga
    12181 attccaggag cgactatcaa gagctgccgc tcatatcaga gcactcgggc ctatcagaaa
    12241 tgcgatccag gaggcagaat cccagtttgc aaggattgaa tcagggaata tcagtttgga
    12301 aagtaacaag gggcttgtcg atttcttcca aaaccgacag ctttgtctta cccaattagc
    12361 cgtccttgtc agtatcctgt cctatcttga aagaggagga ggaagtcgag gttcctacct
    12421 ggttttggat gaagacgggg aaccgatact ggagaaactg ggcttcgaat ggaaatttaa
    12481 acctgagcta aaagaattac gtgattatgt tcaagtgtac agatatgaaa agggtgagca
    12541 tctgatcgag tgggagactg tccgcccaat tcctgaagat aattattggt ttgagaatgt
    12601 atggcaatcc catttgggaa aggacatata tcaagatgaa aagggagaat gaattcaacc
    12661 cgagatggtt ctgggctaca tcatcttcga aatattctga cggaccctga aaatgatctt
    12721 cctgaatata cgttctcggc ccaagcggtc acttgacaga aggtgctggt agtacagcaa
    12781 tgtaattcgg atttacggac atacaagctc gagcggattt aattctggga aatgagggtg
    12841 ctatgagcga acaaaggagc aaaacacttc aacttctcga gaggacgttt gcgatcctgg
    12901 attacatatc ccttgcggaa aaaccatgca tgcttacgga aattgtagaa aagaccggaa
    12961 tttccaagcc atcggtgcac agaatcctct ctacccttca ccattacaga atcgttgtta
    13021 aagacaggca caatcaatac aggatgggac caaaaattat ccagtggggt agagcgtgca
    13081 aaggggattc caatctgttg agtattgctt ccccctatat tgaaaaaata tggaacatta
    13141 ctggtgaaac aattcatctg gtgagtttcg aagacgggta cgcctactat ctgatgaaga
    13201 aagaaagtag atatcctctc cagatgagat ccagaagagg ggacaccata ctgctccatt
    13261 ccactgcggc aggaaaagca attctgtttt cgctaccctt cgacgagctc ctttcttttt
    13321 tgaaaagaac acccctggat aagcggacgg agaatactat taccgatcca aagatattca
    13381 tcaaacaaag ggaagagttc atgtccaaag gatttaccga ggaggaagag gaaaacgaga
    13441 gggatatccg ctgtattggt gtccctattt tgaacgacaa aggcgaccct ctgggcgctg
    13501 tgagtatcac ctgtccaatc ttcctgtgcg acgacaagcg gtctgaagag ctgggggaac
    13561 tgcttaaacg ggaagcaagc aaaatctcgc aggagttcgg ttattcagat tgaggcctta
    13621 ttgaggaaat aggggaagag atccgtgttt tgagcaggta cgatctttca gcaaggggag
    13681 cacctttccc cattgaaaaa attcgtaatt ccacactgct ttttaaagcg gtcgcctgaa
    13741 ctgatcacag taatgagaaa aatgtcctgc ggaagcgcaa tcttcaaagc ttttcacagt
    13801 cctgcccctt ccttgggatg gttctggtga agtctttttt gggggtgatc cttcaatggc
    13861 aaagatagta acttttggtg agataatgct tcgcttgagt cctcctgaac gcgaattatt
    13921 gcttcaaact ccgcagtttg ttgccacttt tggaggagcc gaggcgaacg tggcagtttc
    13981 tcttgcgaat tacggagaag atgtttcttt tgttacggct cttcctgaaa accctatagc
    14041 cgatgcagcc atcgctgaac tgaggaaatt aaaggttggg accgatgaca tcctcagaac
    14101 tggagagagg attggcatct actttgccca ggcgggatcg gtaatgcgtc cttcaaaagt
    14161 agtctacgac aggagccatt cttccatttc gcaggtagag cgtggtgatt ttgattggga
    14221 ccgaattcta caggaagcaa cctggttcca tgttaccggt attaccccgg cgatagcaga
    14281 agggacagcg gaagtcaccc tggaagcctt gggaaaatgc aaggaaaagg ggatcaacat
    14341 ttcctgcgac ctgaactacc gcaagaagct ttggaaatgg ggcaaaccct ctgtggaagt
    14401 catggcggaa gtgacaaaat tcgtcgatgt tttgatcgcc aatgaggagg attgccaaaa
    14461 atgcctgggg atagaggttg acgttgatgt cacgtctggt tcgctggacg ttgataagta
    14521 caggaacctg gcctccaggg tcatggaaac ctacaccggg atcaagtatc ttgccgtaag
    14581 cttgagagag agcatcagcg cggatcataa caactggtcg ggtgtgttgg ccacacggga
    14641 cggtttccat caaagcagaa aattcaacat aacccacatc gttgatcgga taggtggagg
    14701 agatgctttt ggcgccgggc ttatctgggg tcttaaccac ctcgagaacg agcaggtggc
    14761 tctcgatttc gccgttgcag catcagcgat caaacaaaca atttatggtg attttaacag
    14821 gattaccctc aacgaagtac tgaaccttat gaagggggat gcaagcggca gggtgcaaag
    14881 atagagctct cttctttgta aaggacccct gagagcaaca agggagggaa tatcattatg
    14941 gggaaaaccg agaagttcga aatattgggg agaatacatt cgaagggcat cgttgccgtc
    15001 attcgcgctg aaagcgctga cagcggaaga aagatagctg atgctgtttt tgagggagga
    15061 atacccgcta ttgagttgac catgactgta cccggcgcct tgagtatcat tgagacaatg
    15121 gcggaatact acaaaggcag agacattatt ctcggagcag gtacagttat ggatcccgaa
    15181 acagcacggt tgtgtattct tgggggagca cagtatgttg tttcaccgat cctcaaccct
    15241 gaagttgtga agatgtgcaa tcgctatgga attccggcca tgcccgggat aggaacagtg
    15301 acagaacttg ttaaggcaat ggaactgggt gtggatgtgg tgaaatgctt cccgggagag
    15361 gtgttgggac ccgcctttat caaggccgtt cacgggccag ttcctgaggc aaaaataatg
    15421 cccactggag gtgtctccct gtcaaatatc cagaagtggc ttgaggccgg agcatttgcc
    15481 ctggggatgg gaagcgctct gacaaaaccc gatggtcgaa aagacgatta ccaggcaata
    15541 acccggactg cgcagaaagt aatcagacag atagtggact tcagaaaaac ataggggaac
    15601 ataggcagga acataggtgt cagacctaca cggcctgttg cccaaatagt ttatatcaag
    15661 gcgcaagtgg ctgaataccc ttaaaatag
//
LOCUS       DAOTIQ010000046        14817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4858_length_14817_cov_7.723479, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000046 DAOTIQ010000000
VERSION     DAOTIQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 14817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14817
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4858_length_14817_cov_7.723479"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..951)
                     /locus_tag="P5541_06700"
     CDS             complement(64..951)
                     /locus_tag="P5541_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV71709.1"
                     /translation="MEMVRVSGNTWYVPGPTNPGVFAPGKDAVLIDSGNDESMGRKLL
                     AAVRERGWELNVILNTHSNADHVGGNNFIQKRTGCRVAATRVEAALIGDPWLEPSFLW
                     GAFPFREIRGKFLQAEPSTVTDVIPSAGPILDTGLEAVPLPGHFLDMIGVRTPDGVFF
                     VADSLFSSAILDKYGLVVCFDVAGQESTLDLLEKAEADLFIPCHAEPAKDIAPLVRRN
                     RDALLQAREIILKTCETPSSREDLLAELVRRFGINLTPAQYVLTHITVSAHISFLADK
                     GLLVPLFKDGRMLWGKGPE"
     gene            1028..1906
                     /locus_tag="P5541_06705"
     CDS             1028..1906
                     /locus_tag="P5541_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRV71710.1"
                     /translation="MTDLILIDGHALAYRAYFGVRTRHVTSGGVLVNAVHGFGKLLVS
                     LIEGAGAPAAAVVFDSHAPVFRKSIYPSYKANRPAPPEDFLPQIPLMKEFAKNLGIPV
                     ICEDGFEADDLIASAAVREAHSGGRVTIVTPDKDLFQTLGEGNITIMRPAGGDVFTKM
                     DRSLFRKQFGFEPSSMTDYQALTGDRCDNIPGVAGVGPVTAKGLIRRFPDLETIYESL
                     PSISFSVRSRLERDREMAFLCRSLTRLRLDAPLPECLDAGEPDIPGSRDFCSRNNLAV
                     LSGLCSAFNPWPAGRA"
     gene            1915..3726
                     /locus_tag="P5541_06710"
     CDS             1915..3726
                     /locus_tag="P5541_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRV71711.1"
                     /translation="MFPHRSTFLPVNRDDMGARGWDEVDFVFISGDACVDHPSFANAI
                     IPRFLESRGYRVGIIAQPDWRSLKDFLVMGRPRLGALVSAGNLDSMLSHFTAAKKPRS
                     DDPYSPGGKAGFRPQRATTVYCNRVREAWKDLPLVIGGVEASMRRFAHYDYWSDSVRR
                     SVLVDSRADLLVFGMGERAIGEIADLLSKGVPVGQIRDVAGTCFKSRSEDVPVDSVTI
                     PSFAEVSTDNQAFCEAFRLQHMEQDPFRGRSIVQDNGAWNVVQNSPAIPLSVKEMDDL
                     YNLPYSRSPHPMYESSGGVPAIEEVRFSITAHRGCFGSCSFCSITSHQGRIIQRRSDE
                     SIIREAMTITRMPDFKGYIHDVGGPTANFHVPACSKQTRVGPCPDRECLWPEPCPGLE
                     RSHSAYFSLLRKLRDLPGVKKVFIRSGIRYDYLLASKNPDLIRELCEHHVSGQLKVAP
                     EHVSEEVLGLMRKPGKEVFIRFSEAFDRANRQIGKEQYLVPYLMSSHPGSTLANAVEL
                     AEFLRDIGYHPEQVQDFIPTPGTLSTCMFHTGLDPMTGEQVHVPRSAHEKKLQRALLQ
                     YRSPKNRKLVEEALSLAGRNDLVGNHARALLRPQRRR"
     gene            complement(3732..4178)
                     /locus_tag="P5541_06715"
     CDS             complement(3732..4178)
                     /locus_tag="P5541_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV71712.1"
                     /translation="MRKALVAIDGSSITRPLVKFSFERAKKEKLEGLDFIYVFQPYPA
                     ALFPGMPVPVALEEGHSEATKKALEELVRKEKETIGADKVAVEMIFPYGSPSAEIIRQ
                     AEEFPYRSIFIGHRGMGSFERFFIGSVAARVVEHAPCTVIVFRPRD"
     gene            complement(4207..6084)
                     /locus_tag="P5541_06720"
     CDS             complement(4207..6084)
                     /locus_tag="P5541_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71713.1"
                     /translation="MRIARLLIPLIVVCISLSVSPTPAIASQQVSIGVYQNKPLIFRE
                     GGVVKGIYADFLNAVAKENDWDIRWVDGTFSDNLERLKKGEIDLMAAIAWSAARAGEY
                     DFTRKAVISNWGVFYVPRGIAPDSILGLEGRKIAVVRGDVYYAAFRELALKFGIECFY
                     EEVDDYADVMIEIEGARVQAGLVSRLYGLANERDFLVERTSLFIEPTELRFAATKGRH
                     MTLLQILDFTLVKMRTDPGSPLSMSIQRWLSSDLSREHIPEWVRWLAGIAVGALVLVS
                     LVSVVLRRKVVARTRELLQNRKELAREKTLLEKLFEDSPDALVLEGAEGNVVIRINRG
                     FTTLFGFSSEEACGKTLNELVVPEHLLSEGEELDEMANSGKPVYLETLRERKDGSIVD
                     VSIISVPIPLDGDLWGSYTIYRDITASKEAQKVLKENLAKMRRTWEQTIEVLATASET
                     RDPYTAGHQRRVSALAEAIALEMGLDRDTVDSIRMAGLVHDIGKINVPAEILSKPGAL
                     GDVEFALIRTHPEIGYEIMKGVDLPWRLADIIRQHHERLDGSGYPAGLKDKEIMIEAK
                     IVAVADVVEAMSSHRPYRASKGLEAALMEIESNRGVLFCPDVVDACLRLFRKKGFTFP
                     Q"
     gene            complement(6206..7459)
                     /locus_tag="P5541_06725"
     CDS             complement(6206..7459)
                     /locus_tag="P5541_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRV71714.1"
                     /translation="MNELAPLAPMIAALVAGFLLGLMAAFLLRIMHLKNARDIAEELF
                     RESEAERKDQVDAVVDHVKAAFGNLSLDALSRSTEEFLKLAHSRLQSERERGCHDLDQ
                     KKGLIDRQLEEMTRKMEAVSGLVRDLEKDRSAKFGELADRLREVSERTADLTRTTGTL
                     REALSSSRVRGQWGERMAEDVLRLAGFIEGINYCKQKTLAESGRRPDFTFMLPKGLTL
                     NMDVKFPYDNYISWLEAPNENERTRFCKAFMKDVRIKIKEVAGRDYIDPGQSTVDYAL
                     LFIPNDQIFAFIHEQEPSILDDGLRQRVIFCSPVTLYAVLAVIRQAVDNFTLEQTSNE
                     ILSLFGAFKKQWEEFLKKLEHLGKQIEGAHDDYESLISTRRRRLDKPLERIEEIRTLR
                     NIRIAPMVEDGFESQGELFEDTLEE"
     gene            7554..8135
                     /locus_tag="P5541_06730"
     CDS             7554..8135
                     /locus_tag="P5541_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV71715.1"
                     /translation="MLVLAFNASPRPDGNTAFLLGKALSEIEKEGIRTEMIHIGGRPL
                     RGCTSCGECQKRLDRRCALPDDGINGWIERMIEADGVILGSPTYFADVTSEMKAFIDR
                     AGYVCRANGSLLQRKAGAAVIAVRRCGSIHAFDTINHFFMISQMIVPGSSYWNMGIGR
                     HPGEAALDEEGVSTMETLGKNMAWLLARIASRD"
     gene            8203..11046
                     /locus_tag="P5541_06735"
     CDS             8203..11046
                     /locus_tag="P5541_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV71716.1"
                     /translation="MALSKRKNEPFKGKLEDHLRLLVESSPEGMVICDASDRIVFANP
                     EFCRMFGWDRSEITGIPINQIVASSPDVLEEASMVSSSVLSNPHVIPDTYRTRRNGSK
                     IPVSLLAGPISDGEKVIGVYGIYRDTSPRKQAEAQAKREKAFFVNLFENAPEAIVICD
                     RQEKATRCNRAFTEMFGFSSEEIAGKDLNDLLTNDEELRREARNIDVLAWSGDEPVKV
                     DTWRTRRDGTMIPVRVLQFPFRVDEETLVDYTIYRDRTAEIRAEAALKREKYFFEALF
                     ELSPDAIIVCTREGKILRANGSFYSLFGYEIEDRIIGTDLFELVTKKPESKAEAKELD
                     RRFWEGESIDRDVVRQRKDGTDINLHLNQVLLETDDGDLVDYIVYRNIDDRKKTEAAL
                     RESQQQFLHIVEDQDEFIIRFSPDRILTFANSAYCRRFGKTREELTGRSIFDHVDSEE
                     AERLTKKHFDQLTPDNPTNVDESWSLLPDGEVRLEQWQNRGIFDENGNLVCVQAVGRD
                     ITAQRETEKALRESEKRYAAIVEGQVEMVCRFLPGGEITFVNDAFCSFFGVTREQTLS
                     RPLCLPLGPEGWDALNERKEGLSIQDDPVFVEHPCTIPSGEKKWIRWSRKAIFGEDGS
                     MKEIQVVGRDFTRVKENEEELKHLNDVLRSIRGVNRIIFRHNDSETLIRDICALMIET
                     RGYRNVWIGTLGAGGKLATITEAGLGPDGRPLSETTRNVMLSSWGLSVLENPGVTIQR
                     RVTTITDCPLMDRYEESGIMASRLEYDNRVFGLISVSIPGDIQLDPEEKILFSELAWD
                     VSFALHTIEVTRDREEKTETLLGKARQLELLVDHHQDGLWEWDVQAGRVILNSSYETS
                     LGYAAGEMERSYSASIAKIHPDDRPATLEQLEKVISDKTGGDIYRMVYRMRRKDGNWQ
                     RLLDRGYVVRRDRHGKALRMVGTHTNLSSPEED"
     gene            11052..11723
                     /gene="sdaAB"
                     /locus_tag="P5541_06740"
     CDS             11052..11723
                     /gene="sdaAB"
                     /locus_tag="P5541_06740"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRV71717.1"
                     /translation="MRLADVIGPVMIGPSSSHTAGAARLGGLAKRIWGRPVTEVTLFL
                     RGSFAATSRGHGTDLALLGGLTGMAPDDPRIPDAFSEARKAGISWEFNQENVEGAHPN
                     SVRFVFSDGSGTGMEISGSSIGGGAVVLTEIDGFSLRLSGDLAALVTFHEDRPGVVAA
                     VTGSLSRAGRNIASLNLHRQGRGGRAAMVIELDENYLEGLEEMALATHPAINRVLAIP
                     GGEDN"
     gene            11725..12585
                     /gene="sdaAA"
                     /locus_tag="P5541_06745"
     CDS             11725..12585
                     /gene="sdaAA"
                     /locus_tag="P5541_06745"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRV71718.1"
                     /translation="MWSLAELEEGCSKTGLSFPEVIIARDSERSGASPESIRKTMGSR
                     LIDMERSVREALSGEHRGKFIGDDSTLLETYGSKGSPLSGGLVIEASRIALAVSTYNA
                     SMGRIVAAPTAGSCGIIPGMLFACNSLFPTPREKLVDALVVAGGIGQVIARRATLAGA
                     SGGCQAECGAAAAMGSAALVFLRGGSPEAITHGAGITIKSILGLVCDPVAGLVEIPCV
                     KRNGSLVSLGMICADMALSGIRSAIPPDEIIDAMRSVGNSLPDTLRETGMGGVAATPT
                     GIRLKEEFSS"
     gene            12582..13046
                     /locus_tag="P5541_06750"
     CDS             12582..13046
                     /locus_tag="P5541_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV71719.1"
                     /translation="MRRKEKEITERSQIEAILKRAKMCRVAFFDNEYPYMVPLCFGFD
                     GQSIWFHGFNGGKKMECLRKNPRVCFEVEEDIEIVENPDPCEWTVKYRSVVGFGTATI
                     VEETEAKRAGLDVIVTQFAGHPIHFPDQAMAAVAVVRIDIESMTGRRNGYTE"
     gene            13083..13721
                     /locus_tag="P5541_06755"
     CDS             13083..13721
                     /locus_tag="P5541_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRV71720.1"
                     /translation="MFSAIIAWIVATIGRLGYPGVVALMFLESSFFPFPSEVVVPPAG
                     YLASRGEMNLWLVIAAGIAGSILGALFNYWISLTFGRPFFRKFGKYVFVSEKALDRAD
                     EFFAKHGHISTFIGRLLPGIRQLISLPAGLARMNIPLFLFFTTLGSGIWVVILAFVGF
                     WVGNNQELVHQYMHTATYISIAACAVLGTGYVIHYKRKNKRAGNPQTTDRKQ"
     gene            13909..14661
                     /locus_tag="P5541_06760"
     CDS             13909..14661
                     /locus_tag="P5541_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HRV71721.1"
                     /translation="MPELFDKHGGFRKLHSFTLATIVQIETLRFCRRFLTFDHRKTGD
                     KFYDPKGRQYDQMTQAARSGRQNIIEGSERSSTSKDTEMKLTDVARASLSELRGDYEI
                     FILDNGQLPWSVHSSEAKALNAISLDSAPFSDDIVHESAKHAREQRKKYAPWLDSDDA
                     VVVANAMLIIIGRALNMLKSQIAAQGKAFEETGGFSERLTAKRIEKREKQKTEASPDC
                     PLCGKPMRQRKSAKGNFWGCSAFPDCKGTRPM"
BASE COUNT         3403 a         4240 c         4095 g         3079 t
ORIGIN      
        1 cggtcgcccc gaagcaggcg gagaaacgaa cgctttcagg cgagttgcct ttcggaaagg
       61 acttcactcc gggccttttc cccagagcat tctgccatct ttgaacaggg gaaccagcaa
      121 ccccttgtcg gcgaggaagg atatgtgggc cgatacggtg atgtgggtca gaacgtactg
      181 ggcgggagta agatttatgc cgaatcgccg gaccagttcc gcgagaaggt cctccctcga
      241 ggagggtgtc tcgcaagtct tgagaatgat ctccctcgcc tgcaggagcg catccctgtt
      301 tcggcggaca agaggggcga tatccttcgc cggttccgcg tggcagggga tgaaaagatc
      361 cgcctcagcc ttttccagaa gatccagggt gctctcctgt cccgctacgt cgaagcagac
      421 gacaagaccg tacttgtcca gtatggcgga cgaaaagagg ctgtcggcga caaaaaacac
      481 tccgtcaggc gtcctgacgc cgatcatatc cagaaaatga cccggcagcg ggaccgcttc
      541 aagccccgta tcgagtattg gacccgcgga agggatcacg tcagtaaccg ttgaaggctc
      601 cgcctgcaga aacttgcccc ttatctccct gaaggggaac gccccccaca gaaacgacgg
      661 ctcgagccat ggatccccga tcagggccgc ctcaacccga gttgccgcaa cacggcatcc
      721 ggtcctcttc tggatgaagt tgttcccccc gacgtggtct gcattggaat gggtattgag
      781 tatcacgttc aattcccacc cccgttccct gaccgcagca agcaactttc tgcccattga
      841 ctcgtcgttc ccgctgtcga tgagcaccgc gtccttccct ggcgcgaata cccccgggtt
      901 ggttggcccc ggaacgtacc aggtgtttcc agaaacccgt accatctcca ttgttatatt
      961 tcctcccgac ctgttacgat aatggctgcg ggaaaaacag tcttcccccg cgtgaaagga
     1021 gcccctcttg accgatctta ttctcatcga tggccacgcc ctggcctaca gggcatactt
     1081 tggcgtccgc acccgccatg ttacttctgg cggagttctc gttaatgccg ttcacggctt
     1141 cgggaaactc ctcgtaagcc ttatcgaggg cgcaggcgca ccggcagccg cagtcgtttt
     1201 tgattctcac gctcccgtat tccgaaaaag catatatcct tcctacaagg cgaatcgccc
     1261 ggcccctccg gaggatttcc tcccgcagat acctttgatg aaggaattcg cgaagaatct
     1321 gggaatcccc gtgatctgcg aggacggatt cgaggcggac gaccttatag cctcggcggc
     1381 cgtccgggag gcacactcag gaggacgcgt aaccatagta acccccgaca aggatctctt
     1441 ccagaccctg ggagagggca atatcacgat catgcgccct gcggggggag atgtcttcac
     1501 aaagatggac agatcccttt tcaggaagca gttcgggttt gaaccgtcat ccatgacaga
     1561 ctaccaggcg ctgaccggcg acaggtgcga caacattccg ggagttgcgg gagtaggacc
     1621 agtgaccgcg aaaggcctta tcaggcggtt ccccgatctg gaaactatct acgaaagcct
     1681 cccttccatc tctttttccg taagatcccg cctcgagaga gaccgggaga tggcctttct
     1741 ctgccggagc cttacccggc tccgactgga cgcccccctt ccggaatgcc tcgatgcggg
     1801 tgaacccgac atccccggat cgcgtgattt ctgttccaga aacaatcttg ccgtactctc
     1861 ggggctttgc tcggccttca acccctggcc ggcaggaagg gcctgaggat cgaaatgttt
     1921 ccccatcgtt cgacctttct cccggtaaac agggatgata tgggagccag gggctgggat
     1981 gaggtcgact ttgtcttcat ctccggagat gcctgtgtag accacccgag tttcgccaac
     2041 gccattatcc cgcgctttct cgaaagcagg ggttacaggg ttgggatcat agcccagcca
     2101 gactggagaa gcctgaagga ttttctcgtc atgggaagac cccgcctcgg cgcccttgtc
     2161 tctgccggaa accttgactc gatgctgtcc cacttcaccg ccgctaaaaa gcccagaagc
     2221 gacgacccct actctcccgg cggaaaggcg ggcttcaggc cccagagagc gacaacggtc
     2281 tactgcaaca gggtgaggga ggcctggaag gaccttcccc ttgtgatcgg cggcgtagag
     2341 gcaagcatgc gtcgcttcgc ccactatgat tactggtcgg acagcgtccg caggtcggta
     2401 ctggtggact caagggcaga cctcctggtc tttggaatgg gcgagcgggc aataggagag
     2461 atagccgatt tgctctcgaa gggggttcct gtcggccaga tccgcgacgt ggccggaacc
     2521 tgcttcaagt caaggagtga ggatgtcccc gttgactcgg tcacgatccc ttcttttgct
     2581 gaggtctcaa ccgacaacca ggctttctgc gaagctttcc gccttcaaca catggagcag
     2641 gatccattca ggggaaggtc cattgtccag gacaacgggg catggaacgt cgtccagaac
     2701 tcccccgcga tccccctttc ggtgaaagag atggacgatc tttacaacct gccctattcc
     2761 aggtcgcccc atcccatgta cgagagttcc ggcggagtac ccgccataga ggaggtccgt
     2821 ttcagcatca ccgctcacag gggctgcttc ggaagctgtt ctttctgctc cataacctcc
     2881 catcagggaa ggatcatcca gagaagaagc gacgagtcca tcattcgcga ggcaatgacc
     2941 ataacccgga tgcccgattt caagggatac attcacgacg tcggaggccc gacggcgaac
     3001 tttcatgtcc cggcatgttc aaaacagaca agggtcggac cctgccccga cagggaatgc
     3061 ctctggccag agccatgccc cgggcttgag cgaagtcatt ccgcctattt ctcgttacta
     3121 aggaaactca gggacctccc cggggtcaaa aaggtcttca tccgttcggg aatccgctac
     3181 gactaccttc tggcttcaaa aaacccggat ctgataagag aactttgcga acatcacgtc
     3241 agcggacaac tgaaggttgc cccggaacac gtttcagaag aggtactggg gctgatgcgc
     3301 aaaccgggaa aggaggtatt cataaggttc agtgaggctt tcgacagggc aaaccgccag
     3361 atcgggaaag aacagtacct ggtgccatac ctgatgtcga gccacccggg ctccaccctg
     3421 gccaatgccg ttgaactggc agagttcctc agggatatcg gataccaccc ggagcaggtc
     3481 caggacttca tcccgacccc cggtactctc tctacatgca tgttccatac cggtctcgat
     3541 ccaatgaccg gcgaacaggt ccacgtcccg cggagcgccc acgaaaagaa actccagcgg
     3601 gcccttcttc agtaccgttc accgaagaac cgcaaacttg tggaggaagc cctgtcactg
     3661 gccggcagaa atgaccttgt cggcaatcat gcccgcgccc ttctgagacc acaaaggcgc
     3721 aggtagaaaa gtcagtccct tggccggaaa actatgaccg tgcagggagc atgctccact
     3781 acgcgggccg ccacgcttcc gatgaagaag cgctcgaagc ttcccattcc ccggtgacct
     3841 atgaatatgg acctgtaggg gaattcctcg gcttgccgga ttatttcggc ggaaggactt
     3901 ccgtaaggga agatcatctc cacggcaacc ttgtccgcac cgatcgtctc cttttccttc
     3961 cggaccagct cttcgagagc ctttttggtt gcctccgaat gcccctcttc aagggcgaca
     4021 ggtacgggca ttccaggaaa aagcgccgcc gggtagggct gaaatacata gatgaaatcc
     4081 agcccttcca gtttctcttt ctttgccctc tcaaaggaaa atttcacaag cgggcgtgtt
     4141 atggaacttc cgtcgatcgc cacaagagcc tttctcatct catctcacct ccatagttgt
     4201 tctgtgctac tgagggaacg tgaacccctt cttcctgaag agcctgagac acgcatctac
     4261 cacatcggga cagaagagaa ctcccctgtt gctttctatc tccatcagcg ccgcctcaag
     4321 ccccttcgaa gcgcggtagg ggcggtgaga ggacatcgcc tcgaccacat cggcgacggc
     4381 aacgatcttc gcctcaatca taatctcttt atctttgagt ccagccgggt aacccgatcc
     4441 gtcgagtctc tcgtggtgct ggcgaattat gtcggcaagc ctccatggga ggtctactcc
     4501 cttcatgatc tcgtacccta tctcggggtg agtccttata agggcgaact ccacatctcc
     4561 gagggcgccc ggtttgctga ggatctccgc aggcacgttt attttgccta tgtcgtgcac
     4621 aaggcccgcc atgcggatcg agtccacggt gtcgcgatcc agtcccatct caagggctat
     4681 agcctccgcc agtgcggaaa ccctcctctg gtgaccggcg gtatagggat ctcgcgtctc
     4741 cgaggccgtc gccagaacct ctatggtctg ttcccaggtg cggcgcatct tggccaggtt
     4801 ctctttaagg accttctgcg cctccttcga tgctgttata tcccggtaga tcgtatagga
     4861 cccccaaaga tctccgtcca ggggtatggg gacagaaatg atcgacacat cgacaataga
     4921 accgtccttc ctctcgcgaa gggtctccag atacaccggc tttccggagt tggccatctc
     4981 gtccagttcc tccccctcgg agagaaggtg ttccggcacc acaagttcgt taagggtttt
     5041 cccgcaggcc tcctcggaac tgaagccgaa aagggtcgta aaccccctgt tgatacggat
     5101 cacgacgttc ccctccgcac cctccagcac cagcgcatcg ggggaatctt cgaaaagttt
     5161 ctccaggagt gtcttctccc tggcaagttc ttttctgttc tggagcaatt ctcttgtcct
     5221 ggcgacgacc ttccgccgga ggaccacgct caccagcgag acgaggacaa gggcacccac
     5281 agcaatgccg gcaagccatc ttacccactc gggaatatgt tcacgtgaaa ggtcggacga
     5341 aagccatctc tggatcgaca tgctgagagg ggaccccggg tcggttctca tcttcaccag
     5401 agtgaaatcc agtatctgga gcagggtcat gtgtctgccc ttggtggccg caaaccggag
     5461 ttcggtgggc tcgataaaga gggacgttct ctccacgaga aaatctcttt cgttggcaag
     5521 cccgtaaagg cggctgacca gacccgcctg gaccctggct ccctcgatct cgatcatgac
     5581 atcggcataa tcgtcgacct cttcgtagaa acactctatg ccgaatttca gcgccagttc
     5641 cctgaaggcc gcgtagtaga catcacccct tactacggcg atctttctgc cttccaggcc
     5701 aagtatcgag tccggcgcga tccccctggg cacgtaaaag actccccagt tggagatcac
     5761 ggccttccgt gtgaaatcgt actccccggc acgggcggca ctccaggcta tggccgccat
     5821 aagatctatt tcgccctttt tcagcctttc gagattatcc gagaaggtcc cgtcaaccca
     5881 gcggatgtcc cagtcgttct ccttcgccac ggcgttgaga aaatctgcat atataccctt
     5941 cacaacacca ccttcacgaa agatcaaagg cttgttctgg tatacaccta tggagacctg
     6001 ttgggaggca atagcgggtg tgggagaaac agacagcgat atgcagacaa cgatgagagg
     6061 gatcaaaagg cgagctatgc gcatcgtcgt tccccctctc cacaaacaga ccttacggtc
     6121 tggaatcatc gtgaccattt tatcaattca acaaggcaat gacaacagcg attaattgaa
     6181 aacactgtat ctgtcatcgg tgcgcctatt cctcaagggt gtcctcgaag agttctccct
     6241 ggctctcgaa cccgtcctcc accattggcg cgatccggat gttacggagg gttcgtatct
     6301 cttctatccg ctccaacggc ttgtcaagcc gtctccttct tgtggagatc aacgactcgt
     6361 agtcatcgtg ggccccttcg atctgctttc cgaggtgctc aagtttcttg aggaattcct
     6421 cccactgttt tttgaaagcg ccgaagaggg aaagtatctc gttggaggtc tgttcaaggg
     6481 taaaattgtc caccgcctgg cgaataaccg ccagtaccgc gtacagcgtg acgggggaac
     6541 agaagatgac cctctgacga aggccgtcat ccaggatcga tggctcctgt tcgtggataa
     6601 aggcaaagat ctggtcgttc gggatgaaaa gaagggcata gtccactgtg ctttgccccg
     6661 gatcaatgta atctctcccg gccacttcct ttatcttgat ccttacgtct ttcataaaag
     6721 ccttgcagaa acgggtcctt tcattttcgt tcggtgcctc aagccaggag atgtagttat
     6781 cgtagggaaa ctttacatcc atgttgagcg tgagcccctt gggcaacatg aaggtgaagt
     6841 cgggccttct tcccgattct gccagcgtct tctgtttgca ataattgata ccttcgatga
     6901 atcccgcaag acggagcacg tcctccgcca ttcgctctcc ccactgaccg cggaccctgg
     6961 aactcgaaag agcctctctc agggtcccgg tggtgcgggt caggtccgcc gtcctttcgc
     7021 tgacctccct gagcctgtcc gcaagttcgc cgaactttgc cgagcggtct ttttcaaggt
     7081 ctcgcacaag tccggagacg gcctccattt tcctggtcat ttcctcaagt tggcggtcta
     7141 tcagcccctt cttctgatca aggtcgtggc agcctctctc cctctccgac tgaagacgtg
     7201 agtgggcaag tttgaggaac tcctcggtag acctcgaaag agcatcgagg gagagattgc
     7261 cgaaggccgc cttgacgtga tccacgacgg catccacctg atccttcctc tcggcctcgc
     7321 tctccctgaa aagttcttcg gctatgtctc tcgcgttctt caggtgcatt atgcgaagaa
     7381 gaaatgcggc catcagccca agcaggaaac ccgccacaag agccgctatc atcggggcaa
     7441 ggggagccag ttcattcacg ggacatcatc tccacttttt tcctccatta tatacgccga
     7501 tgtactgtta caattgccgg gtcacagaaa cattacaagg aaggagtgat atcatgctcg
     7561 tattggcctt caacgcaagc ccaaggccgg atggaaacac cgcctttctc ctgggaaaag
     7621 ccctttcaga gatcgaaaaa gaaggtatca ggaccgaaat gatccatata gggggaaggc
     7681 ccctgcgggg atgcacatcc tgcggagagt gccagaaaag actcgacagg cgttgcgccc
     7741 ttcccgacga cgggatcaac ggatggatag aaagaatgat cgaagccgac ggagttatcc
     7801 tgggaagccc cacctacttc gccgatgtga cctcggagat gaaggccttc atcgaccggg
     7861 ccggatatgt ttgcagagcc aacggaagcc ttcttcaacg gaaagccgga gcagcggtca
     7921 tagcagtccg ccgctgcggt tcgatccacg ccttcgacac catcaaccat ttcttcatga
     7981 taagccagat gatagttccc gggtcaagtt actggaacat gggtatcgga agacatcccg
     8041 gggaagcggc cctcgatgag gagggagtct ccaccatgga aaccctgggg aaaaacatgg
     8101 cctggcttct ggccaggata gcctcaaggg actgatacct ccctcatccg tgctagaata
     8161 tttgttgttt tctgaactcc ccggccggag gaggttcatt gtatggcact ctcaaaaaga
     8221 aagaacgagc ctttcaaggg aaaactcgaa gaccatctca ggcttctcgt agaaagttcc
     8281 cccgagggaa tggtgatatg tgacgccagc gaccggatag tcttcgcgaa cccggagttt
     8341 tgccggatgt tcggctggga caggtcagag atcaccggaa tacccataaa ccagatagta
     8401 gcttcctctc cggatgttct cgaagaggcc tcgatggtct catcttcggt tctttcgaac
     8461 ccccatgtca ttccagacac atacaggacg cgccgcaacg ggagcaagat ccctgtctcc
     8521 ctccttgccg ggcccatttc cgacggagaa aaagtgatcg gtgtctacgg aatataccgg
     8581 gacacgtctc ctcgcaaaca ggccgaagcc caggcaaagc gcgaaaaggc attcttcgta
     8641 aatcttttcg agaacgcccc ggaagccata gtcatctgtg acagacagga gaaggcaacc
     8701 cggtgcaaca gggccttcac cgagatgttc ggattctcct ccgaagagat agccggcaag
     8761 gatctcaatg atctattgac caatgacgaa gaactgcgca gggaggcgcg caacatcgac
     8821 gtgctggcat ggtccgggga tgaacctgtt aaggtggaca cctggcggac tcgccgggac
     8881 gggaccatga tccccgtcag ggtccttcag ttccccttca gggtggacga ggaaaccctc
     8941 gtagattaca ccatataccg ggaccgtacc gcagagatca gggccgaggc cgcgctgaag
     9001 agggagaagt atttttttga ggctcttttc gaactctctc cggacgccat catcgtctgt
     9061 acacgggagg ggaagatcct gcgggcgaac ggctcctttt actccctttt cgggtacgag
     9121 atcgaggacc gtatcatagg cacggatctc ttcgagctgg ttacaaaaaa accggagagc
     9181 aaagccgagg caaaagaact ggaccgacgt ttctgggaag gcgaaagtat cgacagggat
     9241 gttgtccgtc aaaggaagga cggaacggac atcaacctcc accttaacca ggtgcttctg
     9301 gagaccgacg acggtgatct tgtcgattac atcgtctaca gaaacatcga cgaccgcaag
     9361 aaaaccgagg ccgcactgag ggaaagtcag cagcaattcc tccacatagt cgaggaccag
     9421 gacgaattca tcatccgctt cagccctgac aggatcctga catttgccaa ctcggcatac
     9481 tgtcgccggt ttggaaagac ccgcgaagaa ctgacggggc gctcaatatt cgaccacgtt
     9541 gacagtgaag aggccgaaag gctcaccaaa aagcattttg atcagctcac gccggataac
     9601 cccaccaacg tggatgaatc ctggtcgctt ctccctgacg gggaggtccg cctggagcaa
     9661 tggcagaacc gcgggatatt cgacgaaaac ggaaatcttg tctgcgtcca ggccgtggga
     9721 agggacatta ccgcccagcg ggagaccgaa aaggctctgc gcgaaagcga aaaaagatat
     9781 gccgcgatcg tggaggggca ggtcgagatg gtctgccgtt tccttcccgg aggagagatc
     9841 accttcgtga acgacgcttt ctgctctttc ttcggggtaa cgagagaaca gaccctttcc
     9901 aggcctcttt gtctcccgtt gggacccgag ggctgggatg cgctgaatga acgcaaggaa
     9961 ggactttcga ttcaggatga tcctgtcttc gtcgagcacc cctgcaccat accctccggc
    10021 gaaaaaaaat ggataagatg gtcaaggaag gccattttcg gcgaagacgg ctctatgaag
    10081 gagatccagg tcgtcggcag ggatttcacc agggtcaaag agaacgagga ggaactgaaa
    10141 cacctcaacg acgtcctcag atccatcagg ggagtcaacc ggataatttt cagacacaac
    10201 gatagcgaaa ccctgatacg ggatatttgc gcccttatga tagagacccg gggctacagg
    10261 aacgtatgga taggcactct gggtgcaggg ggaaaattgg cgacgataac cgaagcgggc
    10321 ctgggaccag acgggagacc tctctccgag acgaccagaa acgtcatgct tagcagttgg
    10381 ggtctgtcgg tcctggaaaa tcctggagta acgatccagc gcagggtgac caccataacg
    10441 gactgtcccc tgatggacag atacgaggaa tcgggaatta tggcatcgcg actggagtac
    10501 gataaccggg ttttcggctt gatctccgtt tcgatccccg gagatattca gctggatccc
    10561 gaggagaaga tactcttttc cgaactcgcg tgggatgtct cttttgcact gcacacgata
    10621 gaggtgaccc gggacagaga ggaaaaaacc gaaaccctcc tggggaaggc ccgccagctg
    10681 gagttgctcg tggatcacca ccaggatggc ctgtgggaat gggatgtgca ggcagggagg
    10741 gtgatcctga attcaagtta cgaaacctca ctgggctatg ccgcggggga gatggaacgc
    10801 tcctactccg cctcaatagc gaaaatccat cccgatgacc gccccgccac cctggagcaa
    10861 ctcgaaaaag tgatttcgga caagaccggc ggggatattt accggatggt ttatcgcatg
    10921 cgcagaaagg atggcaactg gcagagactg ctggaccgtg gttacgtggt tcgccgtgac
    10981 aggcatggca aggcccttcg tatggtaggc acccacacga acctttcatc cccggaggag
    11041 gactaacacc gatgagactt gccgacgtaa taggacccgt gatgatagga ccttcatcca
    11101 gccacaccgc gggggcggca aggttgggcg gcctcgcgaa acgaatatgg gggaggcccg
    11161 tgaccgaggt gactctcttc ctgagaggga gtttcgccgc gacatccagg gggcacggaa
    11221 cggacctggc gctcctcggc ggcctcacag gcatggcgcc cgacgacccg cgcattcccg
    11281 acgccttttc cgaagccagg aaagcgggaa tttcctggga gttcaaccag gaaaatgttg
    11341 aaggcgccca ccccaactct gtgcgtttcg ttttttccga cggaagcggt acagggatgg
    11401 agatctcagg ttcttccatc ggcggcggag cggtcgttct gaccgagatc gatggattca
    11461 gcctgaggct ttcgggagat ctggcagcac tggtgacctt ccacgaagac aggccggggg
    11521 tcgtcgcggc cgtgactggc agcctctccc gggccggacg caatattgcc tctctcaacc
    11581 ttcaccgcca gggacgtggc ggcagggcag cgatggtgat agagcttgat gaaaactacc
    11641 ttgaaggact ggaagagatg gccctcgcga cacacccggc cataaacagg gtcctggcca
    11701 ttcccggagg agaggataac tgacatgtgg tcccttgcag aactcgaaga ggggtgctcg
    11761 aaaacagggc tgtcgtttcc ggaagtaatc atcgcccgtg acagcgaacg ctccggggcc
    11821 agcccggaat ccatccgaaa aacaatggga tcacgcctta ttgacatgga aagatccgtg
    11881 agggaggccc tttcaggtga acacaggggc aagtttatcg gtgatgattc cacccttctg
    11941 gaaacctacg gaagcaaagg ttccccactc tcgggaggcc ttgtcattga agcatcaagg
    12001 atagctctcg ccgtatccac ctacaacgca tccatggggc gcatagtcgc ggccccgact
    12061 gccgggagtt gcggaattat cccggggatg ctatttgcct gcaacagcct cttcccgacc
    12121 ccgcgcgaga aactggttga cgctcttgtc gtggcaggag gaataggcca ggtgatcgca
    12181 aggagggcaa ccctcgcggg agcctccggt ggatgccagg cagagtgcgg cgccgcggct
    12241 gcaatgggct ccgcggccct tgtattcctt cgggggggct ccccggaagc cataacccac
    12301 ggggcaggaa taacaataaa atccatactt ggcctggtct gcgaccccgt cgcgggactt
    12361 gttgaaatac catgcgtgaa aaggaacggc tcgctggtct ccctcggcat gatatgcgcg
    12421 gacatggccc tctcgggaat acgctcggcg atccctccgg acgagatcat cgatgccatg
    12481 aggtccgtgg ggaactctct tccggacact ctcagagaaa caggaatggg cggggttgcc
    12541 gcaactccga ccggcataag gctaaaggag gagttttcat cgtgagacgc aaggaaaagg
    12601 agataaccga aagatcacag atcgaggcaa ttctcaaaag ggcaaagatg tgccgcgtgg
    12661 cttttttcga taacgagtac ccctacatgg ttcccctctg tttcgggttc gacggtcaat
    12721 ctatctggtt ccacggcttc aatggaggga aaaaaatgga atgcctccgc aaaaacccca
    12781 gagtctgctt cgaggtagag gaagatatcg aaatagtgga aaaccccgat ccatgcgagt
    12841 ggacggtcaa ataccgcagc gtcgtaggct tcggcacggc aaccatagtg gaggagacag
    12901 aggccaagcg ggcaggcctt gacgtgatcg tgacacagtt cgccggacac ccgatacact
    12961 tcccggatca ggccatggct gccgtagcgg tagttcgcat cgacatagaa tccatgaccg
    13021 gcaggcgaaa cggctacacc gaatagaccg gaccgaatag accaaaagag gagagagaag
    13081 agatgttttc ggcaataatt gcatggatag tagccaccat tggcaggctg ggctacccgg
    13141 gtgtcgttgc gctcatgttc ctggaatcct cgttttttcc cttccccagc gaggtggtgg
    13201 tccccccggc aggatacctg gcgtcccggg gtgagatgaa tctttggctg gtaatagcgg
    13261 cggggatagc gggaagcatc cttggcgccc tcttcaacta ctggatatcc ctgacattcg
    13321 gccgaccctt cttccggaaa ttcggcaaat acgtcttcgt aagcgaaaag gccctggaca
    13381 gggccgacga attcttcgcg aagcacggcc acataagcac attcataggc aggcttctcc
    13441 ccggcatccg gcaactcatc tcgctcccgg ctgggcttgc aaggatgaac atccccctgt
    13501 tcctcttctt caccactctg ggttcgggga tctgggtcgt gatcctcgcc ttcgtgggtt
    13561 tctgggtagg gaacaaccag gagctggtcc accagtacat gcacaccgcc acctatatat
    13621 ccattgccgc ctgcgcggtt ctgggcacag ggtacgtgat ccattacaaa agaaagaaca
    13681 aaagagcggg gaatccccag acgacagaca ggaagcagta aaagctgaag ggctttgtgg
    13741 cccctggtgt gaccggattg accctgttga ccctgttgac ttgttgtcgt tgaggccaat
    13801 cctgacaacg gggtcaatgg ggttctgtca ttctgaggaa tgaaatagtg atctttatta
    13861 caaaggaatc gtcgaggtcg acgggcttcc aactttcggg aggtagttat gcctgagctg
    13921 ttcgataagc atggaggttt ccgcaaatta cactccttca cccttgcgac gatcgtgcag
    13981 atcgagacgt tgcggttttg ccgccgattt ctgaccttcg atcaccgcaa gacgggcgat
    14041 aaattctacg accccaaagg ccgtcaatac gaccagatga cccaggccgc ccggagcggc
    14101 cgccagaaca tcatcgaagg atcggagaga tcctcaacct caaaggacac cgaaatgaaa
    14161 cttaccgacg tcgcacgggc cagcctcagc gaactccgcg gcgactacga gatcttcatc
    14221 ctcgacaacg ggcagctccc gtggtcggtc cactcgtcgg aagcgaaagc actgaatgcc
    14281 atctcgctgg attcggcgcc cttctccgat gacatcgtcc acgagtctgc gaaacacgcg
    14341 cgggagcagc gcaagaagta tgccccgtgg ctcgattccg acgacgccgt ggtggtggcc
    14401 aacgccatgc taatcatcat cggccgcgcc ctgaacatgc tcaagagcca gattgccgca
    14461 caaggcaaag cctttgaaga gacaggcggc ttcagcgaac gactcaccgc aaaacgcatc
    14521 gagaagcggg aaaagcagaa gacggaagcc tcgcctgatt gcccgctatg cggaaagccc
    14581 atgcgccagc gcaaatcggc gaagggcaat ttctggggtt gctccgcctt cccggattgc
    14641 aaaggaacga ggccgatgtg ataaagaaaa agaagctctt gttgaccctg ttgaccttgt
    14701 tgacttgttg accctgttga ctttggggtc aatcctgtcg gcagggtcaa tgggatcttg
    14761 tcagagagtg cgtaatattt tgtgtaaagt ccactgtcgg ggttgagtcc tagtgtt
//
LOCUS       DAOTIQ010000047        14658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_4929_length_14658_cov_8.250976, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000047 DAOTIQ010000000
VERSION     DAOTIQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_14658_cov_8.250976"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5541_06765"
     CDS             <1..187
                     /locus_tag="P5541_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRV71722.1"
                     /translation="VLDEIGEVGDRWGELGLGEPELWPYGHHGKNLYLVKMIKQKPCP
                     ERFPRNPGKANKSVWWR"
     gene            193..963
                     /locus_tag="P5541_06770"
     CDS             193..963
                     /locus_tag="P5541_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV71723.1"
                     /translation="MKTIAVANQKGGVGKTTCCVNLAAELGRQGKKVLLLDIDPQGNS
                     TSGLGVDASNIKKSMYHVILDEVPPTSAIIPSPWEGVWVLPATIDLAGSEVELAGVMS
                     RETRLARHMNSMKDFDIGFIDCPPSLGLLTVNALVAADSLMVPIQCEYYALEGVGQLM
                     RTVSLVQRYLNEDLALDGVVLTMFDSRTNLSREVADEVKNQFKEALFETIIPRNIRLS
                     EAPSFGKPVCYYDPGSAGAVAYRNLAGEVLDRWLAQER"
     gene            942..1829
                     /locus_tag="P5541_06775"
     CDS             942..1829
                     /locus_tag="P5541_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRV71724.1"
                     /translation="MARTRALGKGLGALIPGAGTNYEESSQITSEVLELALENIKPSP
                     NQPRKEFSREGLEALAESIREHGVVQPVVVRRLNGGYEIVAGERRWRAAGMAGLEMIP
                     VRVIEADEHKVMELSLVENLQREDLSPLEAAKGIQELIHRFSLTQEQAAKKLGWSRVA
                     VTNKLRLLQLPDEVLKMLHAGSLTEGHARALLSIELEEERVHFARQCAEKGISVREME
                     EMVRKTTKDIGTPLKKSKQTVLIPEPVLRVSERYGIEVKMTGKGDKVRVLLAGLSEKE
                     AERLFEMIDRSGEQLFPGK"
     gene            1850..2071
                     /locus_tag="P5541_06780"
     CDS             1850..2071
                     /locus_tag="P5541_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71725.1"
                     /translation="MGRTGEDLAAFSRVLSSALLLCGFIVFGVAGGRALVSRGYPGWT
                     LPAAIAAGTLFGAWQAWSFLKTGIKKRDK"
     gene            2620..3114
                     /locus_tag="P5541_06785"
     CDS             2620..3114
                     /locus_tag="P5541_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRV71726.1"
                     /translation="MEQLFAPWRMAYIDKADKQEGCIFCDFPKENKDRERLILARGKK
                     VFVILNAFPYNPGHLMVAPYRHEGEYSLLTEDEFIEMHRFGQRCIEVLRKGMNPQGFN
                     MGINLGKIAGAGFAGHVHLHVVPRWNGDTNFMPVTGKTKVLPVSLDETWESLRKVWEE
                     HVGK"
     gene            3101..3757
                     /locus_tag="P5541_06790"
     CDS             3101..3757
                     /locus_tag="P5541_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRV71727.1"
                     /translation="MSENDRYIEPARETEAELKEKRSLFIGRVIRAYDEIGAKDAVKE
                     VSRKFPDATHNCWAYRVGFPQTVEYYSDAGEPSGTAGKPILGAILRSGLVNVVVVVTR
                     YFGGTKLGVRGLIDAYGKSASMALEAAGKRNRVQSRLAFLRCPYEHAQMVTRQLVDLG
                     IPEEEISHDWGEMVGLRVSVPVSLAKDAESLFEGYGERGLITNWKWQNRLEGSFDDVS
                     "
     gene            3744..5249
                     /locus_tag="P5541_06795"
     CDS             3744..5249
                     /locus_tag="P5541_06795"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRV71728.1"
                     /translation="MMFHEDHSSFSEKLRHPVSRSLLRDLSDEELRAMAAHELVPNNQ
                     GSSLYVTRIRSRSGAFTEVVYEIDEALVALLEQVWGYLRWQQLIRVTARIGSADRQPL
                     FTNMYVTRRYARIAQMFAMNFAVEFDQDYADLVTVVVPEWHQRKVIVMPRLRTTYILG
                     SDYYGEAKMATLRMAMHLARETMNALGLHAGSKVVKVNTPRGLEEKGVLVFGLSGTGK
                     TTITTTDHHLVEPEGVEVLQDDINILLPNGSALGSEANFYIKTDNVTKQPPLLSAARE
                     RCALIENCWVDEDGEIDFNNHAITTNGRAVVPRVAISNTSSRIDLERVDCLLFNMRRY
                     ELPPIGRLVSPEQAAAYFMLGESTITSAEDPSRVGQSKRVPGFDPFIIDNPQLNGNRL
                     LRILRDNPGITCYVLNTGRIGGKDGANMTPEVTFTAIKGALRESIEWGYDDILGYEIP
                     KALDIPDAENYDPYKWYSREQYAEMIGALRRERKEYLVKFKGLAPEVIDGV"
     gene            complement(5823..5969)
                     /locus_tag="P5541_06800"
     CDS             complement(5823..5969)
                     /locus_tag="P5541_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71729.1"
                     /translation="MRERSTEKAEGQAIARKSARAGEKALPKAQEPVSFAAVAEGQEK
                     HTCR"
     gene            6184..6729
                     /locus_tag="P5541_06805"
     CDS             6184..6729
                     /locus_tag="P5541_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRV71730.1"
                     /translation="MRFPAKTMTLTALFAVLTAVGAFIRVPFPPVPITLQTLFVFLSG
                     TLLGSRMGALSQILYVGMGLIGLPVFTGGGGPQYILHPTFGYLLGFIAGAWTIGFISE
                     RARKNSFTVFMIACLSGTAVIYSLGAAGLYINLNYVAGKATTLRQVLEFGVIPFVAGD
                     ILKMLGTAVLASRVKDRLTRV"
     gene            6841..7716
                     /gene="yedA"
                     /locus_tag="P5541_06810"
     CDS             6841..7716
                     /gene="yedA"
                     /locus_tag="P5541_06810"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HRV71731.1"
                     /translation="MDSNLGTARSRVFIAMAVLYAVWGSVFLANRFSLESFPPFMLNA
                     VRFLSAGSFLYFFLRLKGERPMSFQGWLESFLVGALLFIGGAGLLCVGQQWVASGLSA
                     TLIATVPLWTVLFAGIWGKLPTRMEWTGLALGICGVALMNMEKGISGEPLGALALIGA
                     AVSWGLGSVLNNRFRELGRPIASATEMIAGGVLLLAVSLALGEKVSLPLTTKAIMGMI
                     HLSVFASIIGFSIYRYLLRTVRPALATSYALANPVTATILGTLFAGESISLVALASMG
                     VILAGVTLVFRARSI"
     gene            8315..9040
                     /locus_tag="P5541_06815"
     CDS             8315..9040
                     /locus_tag="P5541_06815"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRV71732.1"
                     /translation="MSLETIAECLTVGKTAVLTGAGMSTASGLPDFRGKDGLWKNRDP
                     RLLASISAMDNNRDEFVEFYRHRIRMLQGVEPNEGHRILAGWERMGLLEGIITQNVDG
                     LHQAGGSKNVHELHGSLKVVRCSSCRARHDSEVFLERADCPECGGMLRPGVVLFGEML
                     PVGPMEAAGELSEKASCFLVLGSSLEVSPANGYPMIAKRSGAKLFIINMTPTPLDHLA
                     DEVIHNDIVDALTNIDKYLNRES"
     gene            complement(9243..10070)
                     /locus_tag="P5541_06820"
     CDS             complement(9243..10070)
                     /locus_tag="P5541_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRV71733.1"
                     /translation="MTICYADGRFTEIEKASLPLSDLAFQRGVGVFETIRIYDGHPMA
                     MTTHLQRLADSARGCRIALPLAIEEMKKIILEGAAQYSKNGRARPFITGGDILDREKG
                     FTSPRFFVFFEPLDAPSQSSYTEGVALHPVDASRPMPSIKSINYLGSYLPLTDDPEAL
                     EILYCPDGHITESSHSNAFMVAGGKIITAPLDRVLGGTMRNMAIEIARDEGFTVLERC
                     PLVKELSECAEFFITGSVKEILPVVRIGNNRIGTGKPGPVSAHLRKTYLQNLERWLE"
     gene            10213..11301
                     /gene="buk"
                     /locus_tag="P5541_06825"
     CDS             10213..11301
                     /gene="buk"
                     /locus_tag="P5541_06825"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRV71734.1"
                     /translation="MTAEVLAINPGSTSTKIAWFAGEERIWQETVNHDPGEISAFEKV
                     ADQYEFRKQTIEDSVLKHGSSLDDLDAVVGRGGVLDPIPGGTYGVDEELISDLRRGKP
                     WEHASNLGGIIADAIARPRDIPSYIVDPVAVDELDDISRLTGLPEFPKRSLGHALNIK
                     ATVRRAAKELGRSWDDLDVIVAHLGGGITVCPHRHGRMIDLNNANEFGPFSPERAGGL
                     PAGDLARLCYSGDYNMLDLRKRLVGNGGLKAYLGTSDMREVSSLVKEGDLKAGLVYRA
                     MALQISKEIGACSVALFGKVDAILLTGGVVHDSSFVELIMSMVQWIAPVLVYPGEDEM
                     AALAQGALRVLSGEEKALVYRDCVLRRV"
     gene            11306..12211
                     /locus_tag="P5541_06830"
     CDS             11306..12211
                     /locus_tag="P5541_06830"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HRV71735.1"
                     /translation="MSFRTLDFLLERSMTGKTMVLAVAAPHGEDILGAVAEAKEKGVV
                     SPILIGERKKMEKIAEEHSIDLSTFNIIEEPDDGKAAELAVRTVSSGRADLLMKGNVK
                     TATLLKAVLDKEWGLRSGSLLSHVFLFQVPKIDRVICMTDGGMSMYPDLKAKQGIIEN
                     AVSCYHKLGVECPKVAALAAVEVVNTDMPCTIDAAVLAQMNRRGQIKGCLVDGPLALD
                     NAVSEESARHKGIVSDVAGHADILLVPDIEAGNLIGKVMLYMSGGRGAGVIVGARKPI
                     VLTSRFDTAETKLLSIAFGAVLAKG"
     gene            12739..13662
                     /locus_tag="P5541_06835"
     CDS             12739..13662
                     /locus_tag="P5541_06835"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HRV71736.1"
                     /translation="MKRITSLVELLAYSKEIGPKKIAVALAEDAEVMEAVENARKEGV
                     ANAFLVGDADKLKKVTDQMGIDLANYEVVDVKEGQAAIGVEAVKLVSSGKADILMKGM
                     ISTANFLRGVLNKEVGLRSGGTLSHVYIHQVEGFDRLFFICDPAFNISPDIHTQVNII
                     NNTVGLAHAFGIDVPKIAALAAVEVVNHDMPCTVDAATLTQMNRRGQIKGCLIDGPLA
                     LDNAISEESAKHKGIVSDVAGKADILHVPDIEAGNMLAKAIVYFSNNKTAGLVLGAKA
                     PVVLTSRADSPETKLLSIASAVVLSAYGKKK"
     gene            13691..>14658
                     /gene="buk"
                     /locus_tag="P5541_06840"
     CDS             13691..>14658
                     /gene="buk"
                     /locus_tag="P5541_06840"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRV71737.1"
                     /translation="MESLFILAINPGSTSTKVALFKGTEEIWSDTQRYDAARIREFPN
                     IPAQESFRLEEIRKALGRMKTDVGALDAVVGRGGLLRPIPGGTYAVNVAMLEDLRSCR
                     FGSHASNLGGPLARNLADEAGGKPAFIVDPVVVDELMDEARLSGIPEIERRSIFHALN
                     QKAVARRAAAEIGKPYEEVNLVVAHMGGGISVGAHKKGMVIDVNNALDGDGPFAPERA
                     GSLPAGELVKYAFSGNVELGEIIRKMVGGGGLVAHLGTNDLREVERRIGEGDEQADLV
                     FRAMAYQVAREIGARSAVLSGEVDAVVLTGGLAFSEKFVEAVRERVS"
BASE COUNT         3480 a         3691 c         4267 g         3220 t
ORIGIN      
        1 ggttttagac gagatcgggg aggttggcga tagatggggt gaactgggtc tcggtgaacc
       61 ggaactgtgg ccatatggcc accatggtaa aaacctgtat cttgtaaaaa tgataaaaca
      121 gaagccttgt ccggaaaggt tccccaggaa ccctggcaag gccaacaaga gcgtctggtg
      181 gaggtgacct gtgtgaagac aatagctgta gcaaaccaga agggcggggt cggcaagaca
      241 acctgctgtg tcaatctcgc cgcggaactc ggcaggcagg gaaagaaggt tcttctcctt
      301 gatatcgatc cccagggcaa cagtaccagc ggcctcggtg ttgatgcctc gaacatcaaa
      361 aaaagcatgt accatgtgat actcgacgaa gtcccgccaa caagcgccat cattccttct
      421 ccatgggaag gagtgtgggt tctcccggca acgatcgacc ttgcgggttc cgaagtagaa
      481 ctggctggtg tcatgagccg ggagacgcgc ctcgccaggc acatgaactc catgaaagac
      541 tttgatatcg gttttatcga ttgccctccc tctcttgggc ttcttaccgt taacgctctc
      601 gttgcggccg acagccttat ggttcccatc cagtgcgaat attacgccct tgaaggggtg
      661 gggcagctga tgaggaccgt ctccctcgtg cagcgctacc tcaacgagga ccttgcgctg
      721 gacggggtcg ttcttaccat gttcgattcc cgtaccaacc tttccagaga agtcgcggac
      781 gaggtaaaga accagttcaa ggaggccctc ttcgagacga tcatacccag aaatatccgc
      841 ctttccgagg cacccagttt cggcaagcca gtctgttatt acgatcctgg cagtgccggt
      901 gccgttgcct acagaaacct tgccggggag gtgttggaca gatggctcgc acaagagcgc
      961 taggaaaggg actcggcgcc cttatccccg gggccgggac caactatgaa gagagttccc
     1021 agataacctc agaggttctg gaactggccc tggaaaacat caagccatcc ccgaaccagc
     1081 ccagaaagga attcagcagg gagggcctcg aagcccttgc ggaatccatc agggagcacg
     1141 gtgtcgtcca gcccgttgta gtcaggaggc tgaatggcgg ttacgagata gtcgcgggag
     1201 agagaagatg gcgggccgcc ggaatggctg ggctcgagat gatccccgtc agggttatag
     1261 aggccgacga gcacaaggtt atggagctgt ctctggtcga gaacctgcag agggaagatc
     1321 tttcccccct cgaggcggct aaagggatcc aggaactcat ccacagattt tctctcacac
     1381 aggaacaggc agcaaagaaa cttgggtgga gccgtgttgc tgtaacgaac aagttgagac
     1441 ttctccaact ccccgatgag gttctgaaga tgcttcatgc agggtcgctt accgaaggac
     1501 atgccagggc acttctctcg atagaacttg aagaggaacg ggtccatttc gcccgacagt
     1561 gtgcggagaa gggaatatct gtccgcgaga tggaagagat ggtccgaaag accacgaaag
     1621 atattggaac acccctaaaa aaatccaagc agacggtact gatacctgaa ccggtgctga
     1681 gagtttccga gcgttacggg attgaagtca aaatgaccgg gaaaggtgac aaggtcaggg
     1741 tccttctcgc tggcctgagt gaaaaagagg ccgaaaggct tttcgagatg attgacagaa
     1801 gtggggagca attatttccc gggaaataaa attctttggg aggtgtccca tgggcaggac
     1861 gggcgaagat ctggcggcat tttccagagt tctatcttcg gcgcttctcc tctgcggatt
     1921 cattgtcttt ggagttgccg gaggaagggc ccttgtttca aggggttatc ccgggtggac
     1981 ccttccggcc gcaatagccg ccggaacact tttcggagcc tggcaggcct ggagttttct
     2041 gaaaacagga atcaaaaagc gtgacaagtg agtcgtgaat cgtaaaggca aggaatcagg
     2101 agactggata caggaggcag gaagggcaag gacgggaagg gcaagggcgt gagacgtgag
     2161 acgttagacg aaaaaatcaa aggacaaaaa cagatccttc gatcgctacg ctcctcagga
     2221 tgacaagcat caggtcaagg gcgtgaacgg tgaacggtga acgggaaggg caagggcgtg
     2281 agacgtgagg agtgaggcgt gaggagtgag gggtttttaa acccacaaaa agatccaggt
     2341 tttctctgtg tggtttcacc ctctgtgtcc actgtggtga gaggaaccgt aagtcgtgaa
     2401 tcgtgaatcg aaagatcaaa caccaaaacc agattcttcg tcccgttggt cctcagaatg
     2461 acaacaaggt caagggcgta aacggtgaac agtaaatgac ttgactttga ccctggcatt
     2521 ctgtacttac cattcaccat ttaccgttga ccgtttaccg gtttttgtcg ttgtttcacg
     2581 tgaaacatgc atcttccggc ctgacaggag gggatttttt tggagcaatt gttcgcgccg
     2641 tggagaatgg cctatatcga caaggctgac aaacaggagg ggtgcatttt ctgcgacttt
     2701 cccaaagaga acaaggatag agaaaggttg atcctggcgc ggggtaaaaa ggtgttcgtc
     2761 attctcaacg ctttccctta taaccccggg catctcatgg ttgcccccta cagacacgaa
     2821 ggagagtatt cccttctcac ggaagatgag tttattgaaa tgcatcgttt cgggcaaagg
     2881 tgcatcgagg tcctgaggaa agggatgaac ccccagggat tcaacatggg aataaatctg
     2941 ggaaagatag cgggggccgg ttttgcgggc catgtccatc ttcatgttgt tcccagatgg
     3001 aacggggaca ccaacttcat gccggtgaca ggcaagacaa aggtcctccc tgtatccctt
     3061 gacgagacct gggaaagcct caggaaggta tgggaggaac atgtcggaaa atgaccgtta
     3121 tatagaaccg gcaagggaga cggaagccga gttaaaggaa aaaaggtccc tttttatagg
     3181 gagagttata cgagcctacg atgaaatcgg agccaaagat gcggtaaaag aagtgtcaag
     3241 gaaatttcct gatgcgactc acaattgctg ggcctacagg gtgggtttcc ctcagaccgt
     3301 agaatactac tccgatgccg gggaaccttc cggtaccgcc ggaaaaccta tactaggggc
     3361 gatcttgcgt tctggcctgg tcaacgtggt tgtggttgtc acacgttatt ttggagggac
     3421 caaactcggt gtgcgggggc ttatagatgc ctacggcaaa agtgcgtcta tggctctaga
     3481 ggcggccggg aagaggaaca gggtccagtc gagattggct tttctgaggt gcccctacga
     3541 gcatgcccaa atggtaacaa ggcaattggt ggacctggga atacccgagg aagaaatttc
     3601 ccacgactgg ggggaaatgg ttggcttgag ggtgtcggtc ccggtatccc tggcaaagga
     3661 cgccgaatcc ctttttgagg gatacggcga aagaggatta atcaccaact ggaaatggca
     3721 aaaccgactg gagggatctt tcgatgatgt ttcatgaaga ccactcgtcc ttttccgaga
     3781 aactcaggca tcctgtaagc aggagcctgc tcagggatct gtccgatgaa gaactcaggg
     3841 caatggcggc ccacgaactt gttcccaaca accagggctc ctctctgtat gttacaagga
     3901 tcaggtcccg gagtggtgcc tttacggagg ttgtctatga gatcgacgaa gcccttgtgg
     3961 cccttctgga gcaggtctgg ggataccttc gatggcagca gttgatccgt gttactgcaa
     4021 ggataggttc ggcggaccgt cagccccttt tcacaaacat gtatgttacc cggcgttatg
     4081 cccgaatagc ccagatgttc gccatgaatt ttgccgttga gttcgatcag gactacgccg
     4141 acctcgtaac ggtggttgtt ccagagtggc atcagagaaa ggtcattgtc atgccccggc
     4201 tcaggaccac atacattctt ggaagcgact attacggaga ggccaagatg gcgaccctgc
     4261 gtatggccat gcaccttgca agggagacta tgaatgctct cgggctgcac gcgggaagca
     4321 aggtggtaaa agtgaacact ccgcggggcc tcgaagaaaa gggggtgctg gtcttcggcc
     4381 tctcgggaac aggaaaaacg acaataacca ccaccgatca ccaccttgta gaacccgaag
     4441 gagtggaggt tctccaggac gacatcaaca tccttcttcc aaacggaagt gccctcggct
     4501 ccgaggcgaa cttctacatc aagacggaca acgtgacgaa gcagcctccg ctgctgagcg
     4561 cggcgagaga gagatgtgcc ctcatcgaaa actgctgggt cgatgaagat ggagagatag
     4621 atttcaacaa ccacgccata accactaacg gaagggccgt ggtccccaga gtcgccatct
     4681 cgaacacttc gtccaggata gacctggaaa gggtcgattg tctcctgttc aacatgcggc
     4741 gctacgaact acccccgatc ggacgtctgg tctccccgga gcaggccgcg gcttatttca
     4801 tgcttggaga atcgacgata accagcgccg aggatccctc cagggtcggc cagtccaaga
     4861 gggttcccgg attcgatcca ttcatcatcg ataatcccca gttgaacgga aacaggttgc
     4921 tgcggatact gagagacaat ccgggtatca cgtgctacgt gctcaatacc ggcaggatag
     4981 ggggcaagga cggggccaat atgaccccgg aggttacctt caccgccatc aaaggcgcgc
     5041 tcagggaaag catagagtgg ggttatgatg acattctcgg ttacgagatc ccgaaggccc
     5101 tggatattcc cgacgcagag aattacgacc catacaagtg gtactccagg gagcagtacg
     5161 cggaaatgat cggtgccctc cgcagggagc gcaaagagta ccttgtcaaa ttcaagggcc
     5221 ttgcccctga agtgatcgat ggggtttagg agccgtgaag cgtaagtaga aaaggcaagg
     5281 aagcaggaga ctggatacag gaggcaggaa gggcaagagt aagggcgtaa acggtgaacg
     5341 gtgaacggtg aacgggaagg gcaaggccag atccttcgat cgctacgctc ctcaggatga
     5401 caagcatcag gtcaagggcg taaacggtga acggtgaacg gtgaacggaa tgggcggaaa
     5461 agcaaggaat caggagactg gatacaggag gcaggaaaat cttaggttcc gggttctcac
     5521 aaagaaaggt tttctctgtg tggtttcacc ctctgtgctc tccgcggtga gaaggatttg
     5581 ggtcttgccc ttgcccctca caaagaaagg ttttctctgt ggtgagaagg tctcagggtc
     5641 agatccttcg tccccttggt cctcagaatg acaagcataa gcaaggagac tggcatctgg
     5701 aggcaggaga ctggaagggc aagatcgtga gccggagtga agtctttccc gaaaggcaac
     5761 tcgcctgaaa gcgttcgttt ctccgcctgc tgcggggcga tcgcacagtg ccggtaaaaa
     5821 ccctaccggc aggtgtgctt ttcctgcccc tccgcgacgg ctgcgaaact caccggctcc
     5881 tgagccttcg gtaaagcctt ctctccggcc cttgcgcttt tccttgctat tgcctgtcct
     5941 tctgcctttt ccgttgaccg ttcacgcatt tgatctcgtc tgtcatcctg agcgcagcga
     6001 aggatctggg tttgcctttg actttttcga ttcacgattt gcgattcccg attcacgtgg
     6061 ctaaacctgt ctcccgccac atccccttcc ctttgtctag taattcgaac tccactgagg
     6121 tataatctcc tccatgttat gtcaaccatc ctttttgtaa gttgacaatg gagggacaaa
     6181 attatgcgtt ttcccgcaaa aacaatgact ctaaccgccc ttttcgctgt tctgaccgcg
     6241 gtaggggcat ttatacgcgt tccctttccg cctgttccga taacgcttca gacgcttttc
     6301 gtctttctgt ccgggaccct tctgggatca cgtatggggg ctctttcgca aatactctat
     6361 gtaggaatgg ggcttatcgg ccttccggtc tttaccggtg gaggagggcc gcagtatatt
     6421 cttcacccga cgtttggata ccttctgggt tttatcgccg gggcctggac tataggtttt
     6481 atctcggaaa gagccagaaa aaactcgttc acggtcttca tgatcgcgtg cctttcagga
     6541 actgctgtta tctactccct gggtgctgcg ggactttata tcaatctgaa ttatgtcgcg
     6601 gggaaagcga caaccctgag gcaggttctg gagttcggtg taatcccttt tgtcgccgga
     6661 gatatcctca agatgctggg gacagctgtg ctggcctcga gagtcaaaga tcggctaacc
     6721 cgtgtttgaa tagcagattc cgaataatgg tttgacctcc cgatggaaat agccgggcaa
     6781 agagttaaaa tggccgggaa tcgggccgac ggtctcgtcg ccctccgggg ggctcttcag
     6841 gtggatagca acttgggcac agccagatca agggttttca tcgctatggc cgttctttac
     6901 gcggtatggg gttctgtttt cctggccaac cggttttcgc ttgaaagctt cccgcctttc
     6961 atgctgaatg ccgtgagatt tctttccgca ggcagtttcc tttatttttt ccttcgttta
     7021 aaaggagaaa ggcccatgag ttttcaggga tggctggagt cctttctggt cggggcactt
     7081 ctgtttatcg ggggagccgg gcttctctgc gttgggcagc agtgggtcgc atcggggctt
     7141 tccgctacgc tgatcgcgac ggttccgctc tggacggttc tttttgctgg gatctgggga
     7201 aaactgccca caaggatgga gtggaccggc cttgccctcg ggatttgcgg ggtcgcgctc
     7261 atgaacatgg aaaaggggat cagtggagaa cccctcgggg ctcttgcctt gatcggtgcc
     7321 gctgtaagct gggggctggg ttcggtcctg aataaccgtt tcagggagct tggcaggccc
     7381 atagcctcgg cgacggagat gatagccggg ggggtcctcc tccttgcggt ctcattggcg
     7441 ctgggcgaaa aggtttccct gcccctgacg acgaaagcaa taatgggaat gatccacctc
     7501 tccgtttttg cctccattat cggtttttcc atttacaggt atctgctcag gacggtccgc
     7561 ccggcactgg caacaagtta tgccctggcc aaccctgtca cggcaaccat cctgggaacc
     7621 ctctttgccg gagaatcgat ctcccttgtc gctctcgctt caatgggggt aatactggcg
     7681 ggagtgaccc tggtgttcag ggctagatct atctgaaaag ccgtggatcg aaaaccggaa
     7741 aaatgcgtaa acggtgaacg gaaagggcgg aagagcaagg gcgcgagacg tgagacgtaa
     7801 gacgaaaagg cgaggcggca ggaaacagga gacaggagac aggagacagg aaaatcttag
     7861 gttccgggtt ctcacaaaga aaggtttcct ctctctcgcc cttctctgtg acctccgcgg
     7921 tgagaaggat ttgggtcctg cccttgcccc tcacaaagaa aggttttctc tgtgtggttt
     7981 caccctctgt gctctccgcg gtgagaagga tttgggtcct gctcttgccc cacacaaaga
     8041 aaggttttct ctgtgtggtt tcaccctctg tgctctccgc ggtgagaagg atttgggtcc
     8101 tgctcttgcc cctcacaaag aaaggttttc tctgtgtggt ttcaccccct gtgctctgtg
     8161 gtgagaaggt ctcagggtca gatcactcat ccgcaaaaaa cgcgacctca ggatgacaga
     8221 cagaaccaag gaggcaggag actggcgtct ggtgacagca aggcatgggc aaaagcgtaa
     8281 acggtcgacg gaaattcaaa aaggagggaa agacatgagc cttgaaacga tagcagaatg
     8341 cctgaccgta gggaaaacag cagttctgac cggggctgga atgagcacag cctctggcct
     8401 gcctgacttc cggggtaagg atggcctctg gaagaacagg gatccaaggc tccttgcctc
     8461 catttctgcc atggataaca acagggatga gtttgtggaa ttctaccgcc acaggataag
     8521 gatgcttcag ggcgtagagc ccaacgaagg gcacaggatc cttgcgggtt gggaaagaat
     8581 ggggttgctg gaaggaatca tcacccagaa tgttgatggc ctgcaccagg ctggcggttc
     8641 gaaaaatgtc cacgaacttc atggcagcct gaaagtagtg agatgttctt cctgcagggc
     8701 tcgacacgac agcgaagtct ttcttgaaag ggccgactgc cctgaatgcg gcggaatgct
     8761 ccgccccggg gttgttcttt tcggcgaaat gctccccgtg ggtccaatgg aggcagctgg
     8821 agaattgtcg gaaaaggcct cctgttttct tgttcttgga tcctctctcg aggtctctcc
     8881 cgcaaacggt tatccaatga tagccaaaag gtccggagcg aaactcttca taataaacat
     8941 gacgcccact cctctcgacc acctggccga cgaagtgatc cacaacgata ttgtcgacgc
     9001 gttaacaaat attgataaat acctgaatcg tgagtcgtga gtcgtaaagg caaggaatca
     9061 ggagactgga tacaggaggc aggaaaaaca aaaaaccaat tcagattctt cgctgcgctc
     9121 agaatgacaa gcccttgcct ttcagtttcc cctgtctcca gacgccagtc tcctgcctcc
     9181 ctggtcttcc gcttcacact gttcaccgtt gaccgtttac gcccttgccc ttgccctcgc
     9241 ccctactcca gccatctttc cagattctgc agataggtct tcctcaggtg cgccgatacc
     9301 gggcccggtt ttcccgtacc tatacggtta ttcccgatcc ggacaacagg aagtatctcc
     9361 ttgacgctgc ccgttatgaa aaattccgcg cattcggaga gttccttcac caaagggcac
     9421 ctttccagaa ccgtgaaccc ttcatccctg gcgatctcta tggccatgtt cctcatggtt
     9481 cccccgagaa ccctgtccag aggggccgtt atgatctttc caccggcgac catgaaggcg
     9541 ttgctgtgag aggattcggt tatatggccg tcggggcagt agagtatctc cagcgcttcc
     9601 gggtcatcgg taagggggag gtagctgcca aggtagttaa tgctcttgat gctcggcatc
     9661 gggcgtgaag cgtccacggg atgcagggcc acaccctccg tgtaactgct ttgggacggc
     9721 gcgtcaaggg gctcgaaaaa aacgaagaag cggggactgg taaaaccctt ttccctgtca
     9781 aggatgtctc ccccggtaat aaaaggtcga gcccttccgt ttttggagta ttgcgcggcg
     9841 ccctccagga ttattttctt catctcctcg atcgcaaggg gcagagctat tctgcagcct
     9901 cttgcggaat cggcaaggcg ttgcagatgg gttgtcatcg ccatcggatg accgtcatat
     9961 atccttatcg tctcgaatac gcccactccg cgctggaagg caagatccga aaggggcagg
    10021 gatgcctttt caatttcggt gaaccttcca tcggcgtaac aaatagtcat tgattgtgcc
    10081 tcccgggact ttgtttttac gaacggccaa cagtatactc catttgggtg atcctgaagg
    10141 taaaacagag gtaaaacaag tgtataattt ttcatgaaat aaacgcatat acggattttg
    10201 ggagggaaag cgatgacggc tgaggttctt gctatcaacc cgggatcgac cagcacgaag
    10261 atagcctggt tcgccggtga ggagagaatc tggcaggaga cggtcaacca tgacccggga
    10321 gagatatccg cctttgagaa agtcgcagat cagtacgagt tcaggaaaca aaccatcgag
    10381 gactctgtgc tcaagcatgg gagcagtctc gatgatcttg atgccgtagt agggagaggc
    10441 ggagttctcg accccattcc gggaggtacc tacggggtgg acgaagaact tatctccgat
    10501 ctccgacggg gcaaaccatg ggaacatgct tccaatctcg gcggcattat tgcggacgca
    10561 atagcccggc cccgggatat cccttcctac attgtcgatc ctgtcgcggt ggacgaactc
    10621 gacgacattt cacgattaac ggggctcccg gagttcccaa agaggtctct aggccacgcg
    10681 ctcaatatca aggcgacagt ccgccgggcc gcaaaggaac tgggccggtc gtgggacgat
    10741 ctggatgtga tcgtagccca ccttggcgga ggaataactg tctgtcccca ccgacatggc
    10801 aggatgatag acctgaacaa tgccaacgag ttcggcccat tttcccccga gcgggcggga
    10861 gggctgcctg caggggatct ggcgaggctt tgctacagtg gtgattacaa catgcttgat
    10921 ctcaggaaac gccttgtcgg taacggtggg ctgaaggctt atctcgggac cagtgacatg
    10981 cgtgaagtct cctcccttgt caaagagggg gatctgaagg ccggtctcgt atacagggca
    11041 atggccctcc agatatcaaa ggaaatcggg gcatgctccg tggccctttt cgggaaagtg
    11101 gatgccatcc tcctgacggg aggtgtggtc cacgacagca gtttcgtcga actcataatg
    11161 tccatggtcc aatggatagc cccggtcctg gtctacccgg gggaggacga aatggcagcc
    11221 cttgcccagg gggcgctaag agttctctcg ggtgaagaaa aagccctggt atacagggat
    11281 tgtgtattaa ggagggtata ggtaaatgtc tttcagaact ctcgattttt tgcttgaaag
    11341 aagcatgacg ggcaagacaa tggtcctggc ggtagccgct cctcacgggg aggatatcct
    11401 tggggcggtg gcggaggcaa aggaaaaggg tgtagtctcg ccgatcctca taggcgagag
    11461 aaagaagatg gagaaaatag ccgaagagca ttccatagat ctttcaactt tcaacataat
    11521 tgaggaaccc gatgacggaa aggcggccga gcttgccgtc cggacggttt cctcgggaag
    11581 ggcagacctt ctgatgaagg ggaacgtaaa gacggccacc ctgctcaagg ccgttctgga
    11641 caaggagtgg ggtttgcgct cggggtcctt gctctcccac gtcttccttt tccaggtccc
    11701 gaagatcgac agggtaattt gcatgaccga cggaggcatg tccatgtacc ccgacctcaa
    11761 ggcaaagcag ggaataatcg agaacgctgt ctcctgctat cacaaactcg gcgtcgagtg
    11821 ccccaaagtg gccgccctgg cagccgtgga agtggtcaac accgacatgc cgtgcaccat
    11881 tgatgccgct gtccttgccc agatgaatcg ccgcggccag atcaaggggt gtctcgtgga
    11941 cggccctctt gccctcgaca acgcggtcag cgaggagtcg gccaggcaca agggaatagt
    12001 ctccgatgtt gctggacatg cggacatctt gctggttccc gatatcgagg caggcaacct
    12061 gataggcaag gttatgcttt atatgtccgg tggacgggga gcgggagtca ttgtcggcgc
    12121 ccgaaagccg atagtgctta cgagccgctt cgatacggcc gaaaccaaac tgctgtccat
    12181 cgctttcggc gcggtattgg cgaagggata aattcaagga aacaaggagg caggagacag
    12241 gaaacaggaa acaggaaaca ggaaacagga aacaggaaac aggaggcagg agacaggaaa
    12301 caggaaacag gaaagcaaaa ccaagggcgt caacggtcaa cggtcaacag tgaacgggaa
    12361 gggcagaagg gcctgtcatt ctgaggacca gcgggacgaa gcggaatccg ccagcaggcg
    12421 gacgttccgc gccctttgcc taccggcggg aatctgggcc tgtgttttga ccttccgtct
    12481 cacttcttac gtctaacttc ttacgctctt gcctttgcac accgttcacc gttcacgcac
    12541 ttgaccctgc tgtcatcctg aggagcgcag cgaccgaagg atctgttgtt cttttaaggt
    12601 tttcctgcct cctgtatcca gtttcctgat tccttgcctt tacgattcac gactcacgac
    12661 tcacgattca cggttttgta tcaccctgtt ttcagagtaa aatatacgtt atacatattt
    12721 gttgggaggg ttttgttgat gaaaaggatc acctcgcttg ttgaactgct cgcttattcc
    12781 aaggagatag gacccaagaa gatcgctgtc gccctggccg aagatgccga ggtcatggaa
    12841 gcggtcgaga acgcgcgcaa ggaaggggtc gccaacgctt tcctggtagg cgatgccgac
    12901 aagttgaaga aggtcacaga tcagatgggc atcgaccttg ccaattacga ggtcgtggat
    12961 gtaaaggaag gacaggcggc gataggagtg gaggccgtga agctggtttc ctccggcaag
    13021 gcagacatcc tcatgaaagg aatgatctcc actgccaact tcctcagggg cgttcttaac
    13081 aaggaagtcg gcctgaggag tggtggaacc ctttctcatg tctacatcca ccaggtggag
    13141 ggtttcgaca gactcttttt catctgcgat ccggcattca acatttctcc cgatattcat
    13201 acccaggtca acatcatcaa caacactgtc ggcctggctc atgctttcgg gatcgatgtc
    13261 cccaagatcg cggcactggc cgccgttgag gttgtcaacc acgatatgcc ctgcactgta
    13321 gatgccgcca ctctgaccca gatgaatcgc cggggtcaga tcaagggttg cctcatcgac
    13381 ggtcctctgg ccctcgacaa cgccatcagc gaagagtcag ccaagcacaa gggaattgtc
    13441 tccgatgtag cggggaaggc ggatattctt catgtaccgg atatcgaagc cggaaacatg
    13501 cttgccaagg cgatagtcta tttcagcaac aacaagacgg ccggacttgt cctcggagca
    13561 aaggccccgg tagtgcttac aagcagggcc gattcgcctg agacaaaact tctctcgata
    13621 gcgtcggcgg tagtcctttc ggcctacgga aaaaagaagt aaccggtttt tgagggcagg
    13681 agggttcccg atggaatcgc ttttcatact tgcgataaac ccaggctcaa caagcacgaa
    13741 ggtcgctctt ttcaagggga ccgaggagat ctggtcggat acccagagat acgacgcagc
    13801 ccgaatacgg gagtttccca acatcccggc ccaggagtcg ttccgcctgg aggagatacg
    13861 aaaggccctg ggcaggatga agaccgatgt gggtgctctt gacgcggtgg taggcagagg
    13921 cggtctgctt cgcccgatcc ccgggggaac ctacgccgtt aatgttgcca tgctcgagga
    13981 ccttaggtcg tgcaggtttg gctctcacgc gagcaacctc ggcggccctc ttgcccgaaa
    14041 cctggccgat gaggctgggg ggaaaccggc gttcatagtg gatcccgttg ttgtggacga
    14101 actgatggac gaagcccgtc tttcgggcat tcccgagatc gaaagaagat ccatcttcca
    14161 tgcgctcaat cagaaagccg tagcgaggcg tgccgcagcg gagatcggaa agccttacga
    14221 agaagtaaac cttgtcgttg cccacatggg tggaggcatt tccgtcgggg cccacaaaaa
    14281 gggaatggtc atagacgtca ataacgctct tgacggggac ggcccctttg cccccgaaag
    14341 ggcaggatct ctgccggctg gcgaactcgt taaatatgcc ttctcgggga acgtggagct
    14401 tggagaaata atcagaaaaa tggttggcgg cgggggcctt gtcgcacacc tgggcaccaa
    14461 cgatctcagg gaagtggaaa gacgcatcgg tgaaggagac gaacaggcag atcttgtttt
    14521 cagggctatg gcgtaccagg tggccagaga gatcggagca aggtccgctg tcctgtcagg
    14581 agaagtggac gctgtggttc ttacgggagg actcgctttc agcgagaagt tcgtagaggc
    14641 ggtcagggag agagtctc
//
LOCUS       DAOTIQ010000048        13537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5441_length_13537_cov_8.461282, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000048 DAOTIQ010000000
VERSION     DAOTIQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5441_length_13537_cov_8.461282"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1527)
                     /locus_tag="P5541_06845"
     CDS             complement(79..1527)
                     /locus_tag="P5541_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV71738.1"
                     /translation="MTTILIWSAYALVLILVARLSKGRDVLLPGKVSVLVQALAYVAT
                     YVSAVALVGFAGLCYQWGLQMVLIAAGNMWLGTWFVYRFLAWPTRLWQRKLRAKTPAK
                     MLSNATGSPGIKVYLGLLSSILLVVYASAVFKGAAIMMAGALPVSQTVALWALVGLVA
                     VSVSWGGLRGVLFTEALQGGIMVLGAVALLAATLRAIGGPFEGISALAALAPTAQANN
                     GFASLSSGQGGLFILSLVMVTSVGVWAQPQLVQRHFALKSAKEARRVAPFAMLAIAVV
                     VGGTYFSGAFSRLILGSDAGAVDGIIPLLVNRLLPEAGAQLFALAIISASLSTASALM
                     HIVAAGFGHDVLDRELKGREWQILVALCAIASGVFATRNLQIIAIICTTSWTIIAAAI
                     LAPYLALLAIGDRISKAAVWASSLGGLVSAMVWYAAAYAPTSKGILHMAAPGIIGSVH
                     PFFVSIPVSLIIVAVMTAPEWIGAREKALENS"
     gene            complement(1915..3285)
                     /locus_tag="P5541_06850"
     CDS             complement(1915..3285)
                     /locus_tag="P5541_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV71739.1"
                     /translation="MNNQDTPEYNIFFEKLFENSPEGVVIQGPDGTVQKANRAFCEMF
                     GYNLEEVLGQDLDYLVARDKDIRTEASRRTVDAVSGSQFSFETIRQKKDGTRFHVTAF
                     GVPILKDGKTVAIYGMYRDISDRKNAEEALRRSEERYRAIIEQQRDIIVRFTPDWNIT
                     FANEAYCRYYGLTADKALGRSFLSHIPETDRGKTIKHLQGLSKENPVEKTEEVTILPS
                     GETRWQQWIDTALFDSSGNIVEYQSVGRDITDRVLMEDSLRQSTEKLEKTLDDTVRLL
                     AGTMRIRDVYTADHQEGVARLAKAIAEEMGFSDERVKGIWVAGMIHDIGKLYIPGEIL
                     NKPTSLSNLEYEIVKRHAEVGAETLQEVSFPWPVSRIVGQHHERLDGSGYPNGLVGDE
                     ILEETRILSVADVVEAMRSHRPFRAAYSLDKALDTVREGSGKIFDERVVASCLKIFEK
                     GFEFEA"
     gene            3379..4164
                     /locus_tag="P5541_06855"
     CDS             3379..4164
                     /locus_tag="P5541_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRV71740.1"
                     /translation="MRIAVLIKQVPDTDEVKIDETSGTLVREGTGTIINPLDLHALES
                     AFLLKRFNGASVSVFSMGPMQAETALRESLALGADDAFLLSDPAFAGSDTWATARVLA
                     SALISFGPYDLVLAGEKATDGETGQVGPEVAVMLGVPFSTCVSSFNISGGFANLVRTV
                     EEGTESQRIPLPCLLTVLRDINEPSMPTLAGKMRARRSDISKVDAATINIEHSSVGLT
                     GSPTRVVKVFRPRVTRGAEHFDADNIDKGVERFIEILKERSLL"
     gene            4174..5181
                     /locus_tag="P5541_06860"
     CDS             4174..5181
                     /locus_tag="P5541_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRV71741.1"
                     /translation="MKTGDVMVVGEVRSGVVQPVTMELISRARILADLQGSDVVVLLM
                     SDELESGPGECAAFGADRILLALAPEFGLFNQDIQYRTVVHAVMETKPAVVLAPATTS
                     GRTIMPAAAAALGTGLTADCTGLDIDFDTMDLIQTRPAIGGNVMASIRTPNHRPQMAT
                     VRPRTFSPGEPDRTREAKLLRLDIPQEILDSPVEALGAEPAEGDESNIQDMDVIVSGG
                     KGVARAGSFDLLRELASLLGGGVGASRAAVDNRWIGYPHQVGLSGKAVSPRVYIAAGI
                     SGSVQHLAGIQTADFIVAVNRDPDAPIFRVADLSICGDLFDVIPRMIDAVRSRIEKRK
                     S"
     gene            5178..6602
                     /locus_tag="P5541_06865"
     CDS             5178..6602
                     /locus_tag="P5541_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRV71742.1"
                     /translation="MMGSRSYSPVDEEVIKELEQIFGPGNVSFEPGKIRAYSRDEVGE
                     QFWDRDYLAEAVVFPETTEQVSSLMKLASKRFIPVTPRGAGTGLSGGAVPAFGGIVVS
                     FEKMNRILECDPINLTATVEPGVITADISRAASSHGLQYAGDPCSGDASFIGGNVAEN
                     AGGNKVIKYGSTGANILGLEVVLPSGEVTWFGGKRRKDVTGLDFVHLVAGSEGTLCLV
                     TKIIVKLVPLPECIVDLLVPFRTMEEAISFVPRILTEGRITPASVECMNRNSLEIVSR
                     YTGTALPFQEAGAHLIIQLEGNDPEYLADEYERLGQICLSSGAQEVFVADNRNARDRL
                     WKARKSIAEAVMAFSTRYAKEDVVVPTSEVPELMREVDRIGRKYGLDGVNYGHVGDGN
                     IHVNFLMEEDRPDWKEVIESARMELYDATVRLGGTLSGEHGIGMKRRKYMRTFLDGAH
                     IDLIRNVKKAFDPLEIMNPGKIIE"
     gene            6704..6880
                     /locus_tag="P5541_06870"
     CDS             6704..6880
                     /locus_tag="P5541_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71743.1"
                     /translation="MKRENVIISNLACTSDLCSMVEFDAQPLRDMMDDLESGWVESRA
                     QVPHCDKECGGCGR"
     gene            complement(6952..7353)
                     /locus_tag="P5541_06875"
     CDS             complement(6952..7353)
                     /locus_tag="P5541_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71744.1"
                     /translation="MLYFQFLALIFGTVMLTLAPFLVLRGERWVELFRDIMYPEKQPV
                     WIWVTGAAAIILVLLTWYVELTTKIPYSWIMTVFITLAIPKFYLLTFQYSRTRSFLSA
                     LMDKGRVFTLGMAGVLYLFGFLVICLGIFAF"
     gene            complement(7388..7777)
                     /locus_tag="P5541_06880"
     CDS             complement(7388..7777)
                     /locus_tag="P5541_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71745.1"
                     /translation="MARKKDSAFGKKMILFLAAFAFAFIVSWHFYLDRVSAKAEAIGL
                     VQSQHLPKEKTDEFISKVHEGGKFKLMGWGIEETDEKNVFIVSYTARRLNEDGFKVGD
                     PIGYWFKVDPAEGTCVPLKPEGTVAPD"
     gene            7907..11248
                     /locus_tag="P5541_06885"
     CDS             7907..11248
                     /locus_tag="P5541_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71746.1"
                     /translation="MISKKIGSMAMIALSVAALVGLWSIGIATNAMAAPSWLQVGQPV
                     PDQFTFSGEIPSIEEVRDAFVRVWGNDPEKWPEEFRRIHEIQVAFNKRAMSSSKFKEQ
                     FGRDALEKIKVQREDPVGYWVRSLYDGWGFEEWFNSEHLTVQRVPRALLVRFLEELFP
                     LMWRDIRSSVHVLEFMLGLPDSKFIPYNNYDSQVNDLRKRDVLEYNQSIEKTAERYIR
                     NNDYLMLLLMPDRMDTNWVGDAGFEASLVSRARKLSARVQEAVKNMGDRFRDAFYEAV
                     PRMTYSWNEEPWSENHVVIGGAPSRVSANVDLLGKSTRIEHPYLGDKKTIKEQKVASG
                     GAMKLQWGTAPVHSSEFDRGAVVEKISPRYLIVRKQYGESSEFVLSLYLDHISGFGHE
                     VAFTPAEGPSFPDEIGKEMMEAVTAAFFEAAEILYGESSGRTTLATKDTKSVSPPRAE
                     PGEQSRIKKIVIDPPKSMEIIASGKNNEGTHFNVEVWDERERIPQVEVSVKKPVIGTL
                     SSRNATGGDEKRLIVKTGPLGYAEITYTPPSIPEMKLLGIIQKDIRIVAEDPRSGVTS
                     SMSLRVSRPKDIDISLEHSVLPADPAFTNAVDFRFDGWEQGGDPEEKYRVRIRALSGK
                     GVLSPGGDPSGGRSSLVLELKADQQHRLFYRWMGEGTKGGPVPETLVFEVPELEIKGF
                     VNFSVGFAPGLYSSRQSYSEVEQPGLFVPLQVYVQDEYHPDLDMSEFFRAFELTPVLD
                     ISQIGFEPVATEGSKSREILEDILKHVKGAKLPKDVLSLKPESWSLMRGSGSRWFIVG
                     ETVGEGVSQGKAFPGIIPWEYGDYTFRISMNLEGPSGEAIEPLRQTMTRILHVRPFES
                     GVSEGQAEMILPMILSFSAMFPGEDAQIFAARGRNLLQRGEFETAAVVLGENFSQRLS
                     WQPFPDQPASLEKERLRQLVSQAHGVTGAAVPEETVGDSLSLAKQNFLCTVAGVYAEN
                     FLSWGYDLSLLIDSPQIGTDRPELRLLEMIKGFLEGYGEYGIVAITRKNIESFSVYTE
                     TGERLREFGGQVFGGGSQYERIFCGEHSIVVPFRLGENLVVTFKGTGEPVEAIKILPN
                     GINVQRYGSRPGSETINVYGDVVRP"
     gene            11273..11695
                     /locus_tag="P5541_06890"
     CDS             11273..11695
                     /locus_tag="P5541_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71747.1"
                     /translation="MGEKILKVVNRDRCTGCFSCMYACSRHVKGVGGTGKSAMRVRAY
                     SGTEGAFSIRVCMRCIHPDCAAACPTGALIPAPGGGVRLDREKCVSCGACVRACTIAA
                     LQWDEEERVPIPCIFCGQCVAYCPNGVIALVERTSGGE"
     gene            11698..13473
                     /locus_tag="P5541_06895"
     CDS             11698..13473
                     /locus_tag="P5541_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV71748.1"
                     /translation="MLQGRILHVDLTYGKTHIEEEPELFAKHLGGTAVATELLDRFGR
                     TDLDPLDPRAPVILAIGPFSALYPVATKTVSVFRSPLTGELGESHAGGRLAAALRETG
                     VDAVVIRGASKDPVFLHVDGHEISLRSARTFWGQSSPATDRLLREALEKKGRKLSLVR
                     IGPAGERLSPMACATVDNSRHFGRLGLGAVLGSKKLKAIAVSGKGSVAVENPKSYRDI
                     YHDIYEKVVRSDEMKKYHDLGTPANVLTLNAINGLPTRNFSQGFFENAQKISGEAFLE
                     EVNVQHMACAHCPCGCIQVAELREQFAPSHYSTVRVSYDYELIFAVGTQLSISTPREI
                     LRLIVFIEKQGWDCMSMGNTLAWATEAFLSGVIDENNTAGLSLSFGDTSTYLEVMRRV
                     SEGQGEFFRDLEKGTAFCSSKYGGGDFAMHYGGLEPAGYLTGENAVMTWIAGVRHSHL
                     DDNGYGIDQKLILGEISMEDQVKMQVREAQWRMVLNSLVICLFARGVYDASTISKGLE
                     ALGLDWSPEKLLDLGARALKAKYAWKKKCGFSPERITIPEKMLRVRTSNGLIDRERMK
                     KRLELFLRYAGLPCPCLSSEKRATT"
BASE COUNT         3151 a         3559 c         3814 g         3013 t
ORIGIN      
        1 aaacagtaaa cggaagatgc agaatttatt atttggtttt accgttcacc atttaccgtt
       61 tacgcttttt cagtttctct agctattctc taatgctttt tcccttgccc caatccactc
      121 cggggcggtc atgacggcca cgataatcag cgatacggga atgctcacga agaaagggtg
      181 gacactccct attattcccg gcgcagccat gtgcagtatt cccttcgagg tcggggcgta
      241 tgccgcggcg taccagacca tcgcgctgac cagtccgccc agggacgaag cccataccgc
      301 cgccttgctt attctgtcgc ctatcgcaag aagtgcgagg tatggagcaa ggatggcggc
      361 cgcgattatc gtccagctcg ttgtgcagat tattgctatg atctgaagat tcctggtcgc
      421 gaaaactccg ctcgcgatgg cgcagagcgc caccagtatc tgccactcac gacccttgag
      481 ttcccggtca aggacatcgt gcccgaaacc ggccgcgaca atatgcatca atgctgaggc
      541 tgtcgaaaga gacgccgaga taatggccag agcaaaaagc tgtgcccctg cctcaggtag
      601 aagtctgtta accagaagag gtatgatccc gtccacggcg ccggcatcgg acccgagtat
      661 tagccttgaa aatgctccgg aaaaataggt cccgccgacc accacggcta ttgccagcat
      721 cgcgaagggg gccacccggc gtgcttcctt cgcgctcttg agagcgaaat ggcgttgcac
      781 gagctgaggc tgtgcccaga cgccgacgga tgtgaccatg accagcgaga gaatgaagag
      841 ccccccctga ccggagctaa gagaggcgaa gccgttgttg gcctgtgccg tcggagcgag
      901 cgcggccagg gcacttatgc cctcaaaagg acctccgata gcccttaagg tggctgccag
      961 aagagctacc gcaccaagga ccatgatccc accctgcagt gcctccgtga aaagaacccc
     1021 ccttaaaccc ccccaggaga cgctgaccgc aaccaggcca acaagtgccc atagcgcgac
     1081 ggtctgggag acggggagag cgccagccat cattattgcg gcccccttga atacggcaga
     1141 ggcgtatacg acaagaagaa tacttgaaag aagccctaga tataccttga ttcccggtga
     1201 ccctgtagca ttggaaagca tctttgctgg tgtcttcgcg cggagctttc tctgccaaag
     1261 ccttgtgggc cacgccagga agcgatagac gaaccaggtg cccagccaca tgttccccgc
     1321 ggcgatcaga accatctgca gcccccactg gtagcagagt ccggcaaagc ctacgagagc
     1381 aacggccgaa acgtaggtgg cgacgtaagc aagtgcctgg accagaacgg agaccttacc
     1441 gggaagcagt acgtcccttc cttttgaaag tcttgccaca agaatcaaca caagagcgta
     1501 ggcggaccag ataagtattg ttgtcatgac cgctcaccgt tttttgcgat cttccataca
     1561 gaccagatcg ctccccatat cagtagccct gttgctgctg aaacccagat gtccatctga
     1621 ctcatctctc actcacctcc gcaagataaa aaaagggccg ccgatccgaa gatcggcggc
     1681 cctggttctg ctttaaaaac tgctgttctc ctgttaaagc aacggccgac gtaccccgga
     1741 tccaaatgat tgggggagat agtaatagcc gtaataatag ttgccggcaa acgtcgctgt
     1801 tgcttttgtt ccaggaactg tcatgcaagc ctccaagtgt tctttaataa tttcagagag
     1861 cctatcagta attttatgaa gtgtcaagta tctccggatt gttggcttaa cctgttacgc
     1921 ctcgaactcg aatccttttt cgaatatctt caggcaagag gccacgaccc tctcatcgaa
     1981 gatctttcct gaaccttccc ggaccgtatc aagggccttg tccagggagt aggccgctct
     2041 gaagggcctg tgagagcgca tcgcctccac aacgtccgcg accgacagta tccttgtttc
     2101 ttccagtatt tcatccccaa ccagtccgtt gggataaccc gatccgtcca gtcgctcgtg
     2161 atgctgcccc acgatcctgg agacaggcca ggggaaagac acctcctgga gggtctccgc
     2221 ccctacctcg gcgtgacgct tcacgatctc gtattcaaga ttcgagaggg aagtcggctt
     2281 gttgagtatc tccccgggaa tgtagagttt gccgatatcg tgaatcatcc ccgctaccca
     2341 tatccccttg accctctcat cggagaatcc catttcttcg gcgatcgctt tcgcgagacg
     2401 ggcaactccc tcctggtggt cggcggtgta gacgtcgcga atcctcatcg tgccggcaag
     2461 aagccggacc gtatcgtcga gagttttttc cagtttttcc gtgctttgcc tgagagaatc
     2521 ctccattagt acacggtcgg tgatgtcccg ccccacggac tggtattcga caatgttgcc
     2581 ggaactgtcg aacagggccg tatcgatcca ttgttgccat ctcgtctccc cggaaggaag
     2641 gatggtcacc tcctccgttt tctccacggg attttctttg gagaggccct gcaggtgttt
     2701 tattgttttt cccctgtcgg tttccggaat gtgcgaaaga aaggatcttc ccagggcctt
     2761 gtcagccgtc aggccgtaat aacggcaata ggcctcgttg gcgaaggtga tattccagtc
     2821 aggagtgaac ctgacgatga tatctctctg ctgttctatg atggcgcggt agcgttcctc
     2881 gcttcggcga agggcctcct cggcgttctt gcggtcggat atatcacggt acattccgta
     2941 gatggcaact gtttttccgt ccttgagtat gggaacgccg aaagccgtta cgtggaacct
     3001 ggttccgtcc tttttctgac ggatcgtttc gaaggagaac tggctaccac ttaccgcatc
     3061 gaccgtgcgc cttgaggcct cggttcttat gtccttgtcc cgggccacaa ggtagtccag
     3121 atcctgaccc agaacttctt ccaggttgta gccgaacatc tcgcagaacg cacggttggc
     3181 cttctggaca gtcccgtcag gtccctggat cacgaccccc tcgggagagt tttcgaaaag
     3241 tttctcgaaa aaaatgttat attctggagt atcctgatta ttcactatag gagcccctcc
     3301 tttgcggtgg ggttattact ggaatttagt atatcatggg tcggctttgt gtttttctca
     3361 aggcccggga ggtgttccat gcgtatcgcc gttcttatta aacaggttcc cgataccgac
     3421 gaggttaaga tagacgagac cagcgggacc cttgtaaggg aaggtacagg aaccataatt
     3481 aatcccctgg accttcacgc cctggagtcg gcttttttgc tcaagcgctt taatggagcg
     3541 tctgtctcgg tcttttccat ggggcccatg caggccgaaa cggccttgag agaatctttg
     3601 gctctggggg cggatgatgc ttttttacta agtgatccgg cctttgccgg gtcagatacc
     3661 tgggcaaccg caagggtcct tgcttctgcg cttatctctt ttggccccta tgaccttgtt
     3721 ctggctggtg aaaaggcgac cgacggagag acggggcagg ttggccccga agtggcagtt
     3781 atgttgggcg ttcctttctc aacctgtgtg agttctttca atatatccgg aggttttgcg
     3841 aacctcgttc gaaccgtcga ggagggtacc gagtctcaga ggatccccct tccgtgcctg
     3901 ctgacggtac tccgggatat caatgaacct tccatgccga ccctggcggg gaaaatgcgt
     3961 gcccgacgca gcgatatcag caaggtcgat gccgcgacaa taaacattga acactcatcc
     4021 gtcggcctga ccggctcgcc taccagggta gtgaaggtgt tccgccccag ggtcacccgt
     4081 ggagcggagc attttgacgc agataatatt gacaagggtg tagaacgttt tattgagatt
     4141 ctcaaagagc gttcgctgct gtgaggtgat gcaatgaaaa ccggagatgt tatggttgtc
     4201 ggcgaagtca ggtcgggggt cgtccagccc gtaacgatgg aattaatctc aagggcaagg
     4261 atcctcgcgg atttacaggg gtcggatgtg gtcgttcttc tcatgtccga tgagctggag
     4321 tctggcccgg gcgagtgcgc tgcctttggt gccgacagga tattactggc cctggctccg
     4381 gagttcggcc tcttcaacca ggatatccag taccgaaccg ttgtccacgc ggtaatggag
     4441 acaaaaccgg cggtggttct ggcacccgct acgacctccg gcaggactat tatgccggcg
     4501 gccgcggctg cgctgggaac aggactgacc gccgattgca caggtcttga tattgatttc
     4561 gataccatgg accttatcca gacacgtccc gcgatcgggg gcaatgtcat ggcctcaata
     4621 cgcaccccga accatcgtcc tcagatggcc accgtcaggc ccaggacttt ctctcccggc
     4681 gagcctgaca gaaccaggga agcaaagctg ctgagactag atatcccgca ggagatactt
     4741 gatagtcctg tcgaggccct gggtgcggaa ccggcagaag gtgacgaatc caacattcag
     4801 gacatggacg taatagtttc cggcggcaag ggtgttgcga gggcggggtc tttcgacctt
     4861 cttcgcgaac ttgcttccct ccttggcgga ggtgtgggcg caagccgggc cgccgtagac
     4921 aacaggtgga taggctaccc ccatcaggtg ggtctctcgg gaaaggccgt atccccaagg
     4981 gtctatattg ccgcgggaat atccggctcc gttcagcatc ttgcgggcat tcagacagcg
     5041 gattttattg ttgccgtaaa cagggacccc gacgcaccca tatttcgagt tgcggatctg
     5101 tcaatatgcg gggatctttt cgacgtcatc ccaaggatga tcgatgctgt cagatcccgg
     5161 atagagaaga ggaaatcatg atgggatctc gctcctattc ccctgtcgat gaagaagtaa
     5221 taaaagaact ggagcagatc ttcggacccg gcaatgtctc tttcgagccc gggaagatca
     5281 gggcctactc cagggacgag gttggcgaac agttctggga ccgggactac ctggcagagg
     5341 ccgttgtttt tccggaaacc acggaacagg tctcctcgct catgaaactg gcatcgaaaa
     5401 gatttatccc cgtaacgcca agaggcgcgg gtacgggact ctcgggaggg gctgtgccgg
     5461 cttttggagg gatcgttgtc tccttcgaaa aaatgaaccg catactggaa tgcgacccaa
     5521 tcaacctcac cgctaccgtc gagccggggg tcatcactgc agatatcagc agggccgctt
     5581 cttctcatgg tcttcagtac gccggagatc cctgcagcgg ggacgcttct ttcataggag
     5641 ggaacgtggc ggagaatgca ggaggcaaca aggttatcaa gtacggctcg acaggggcca
     5701 acattcttgg tctcgaggtg gtgctcccat cgggtgaagt gacctggttc gggggcaaga
     5761 gacgcaagga tgttacgggc ctcgattttg tacaccttgt ggcaggttcc gaggggaccc
     5821 tctgcctggt gacgaagatc atcgtgaaac tggttcctct ccccgaatgc atagtcgacc
     5881 tcctcgtccc tttcaggaca atggaggagg ccatatcctt tgtaccaagg atccttaccg
     5941 agggaaggat cacacctgcc tcggtggagt gtatgaacag aaattccctt gagatagtct
     6001 ctagatacac tggtacggcg ctgcccttcc aggaagctgg tgcccacctg ataatccagc
     6061 tggaggggaa tgacccggaa tacctggccg acgaatacga gcgattgggg cagatctgcc
     6121 tttcttcggg agcacaggag gtctttgtag ccgacaaccg caacgccagg gaccgcctgt
     6181 ggaaagcccg taaaagcata gccgaagcgg taatggcttt ttctacaagg tatgccaagg
     6241 aagatgtcgt tgttccgacc agcgaggttc ccgaactgat gagagaggtc gacaggatag
     6301 gcaggaaata tggccttgac ggagtaaatt acggtcatgt gggggatggc aacattcacg
     6361 tgaattttct gatggaagag gataggccgg actggaaaga ggtcatcgaa tcggcccgca
     6421 tggaattata tgatgctacg gtgcgcctgg gaggaaccct ctcgggggaa cacgggatag
     6481 ggatgaagcg ccgcaaatat atgcggacat tcctggacgg ggcgcacatc gacctgatca
     6541 ggaatgtcaa aaaagccttt gaccctctgg agataatgaa ccctggaaag atcattgaat
     6601 agggacaagt cccccgaacc cttgtgtcac atgctctcca tatgatatga taatggcata
     6661 aaattcagaa acaaataaca gttttattct ggaggaataa gacatgaaaa gggaaaacgt
     6721 gataatttcc aacctggcat gcacttccga tctttgcagc atggtggagt ttgacgctca
     6781 gcccctaagg gacatgatgg atgacctcga gtcaggctgg gtggaatcca gggcccaggt
     6841 tccccactgc gataaggaat gtggcggctg cggtcgctga catagcctct tgcgttaata
     6901 aaaaagatcc agcccagaag ggctggatct ttttttggaa actcagacga atcaaaacgc
     6961 gaatattccc aggcagatca caagaaaacc gaacaggtag aggacccctg ccatcccaag
     7021 ggtaaatact ctccccttgt ccatgagtgc tgacaaaaaa cttcttgtcc ttgagtactg
     7081 gaacgtcaga agatagaact tcgggatcgc cagggttatg aaaacggtca tgatccagga
     7141 atacggtatt tttgttgtca gttccacata ccatgtcaag agaacaagta ttatcgcggc
     7201 ggccccggta acccagatcc atacgggctg tttctcgggg tacataatat cgcggaaaag
     7261 ctcgacccag cgttcccctc tcaaaaccag aaaaggagcc agcgtcaaca ttactgttcc
     7321 aaagatcagc gcaagaaact gaaagtacag catttcctct caccctcccc ctggattgtc
     7381 gcctgtttca atccggtgct acggttccct caggtttcaa tggcacgcag gtgccctcgg
     7441 cgggatcgac cttgaaccag tatcctatgg ggtctccaac cttgaatccg tcctcgttaa
     7501 gtcttctggc ggtatagctc acaataaaca catttttttc atctgtctcc tcgatacccc
     7561 agcccatcag tttgaacttt ccgccctcat gcaccttcga gataaattca tcggtctttt
     7621 cctttggaag atgctggctc tgaacaagac ctatggcctc tgcctttgcg cttaccctgt
     7681 ccaggtagaa atgccacgag actataaagg caaatgcgaa agccgcaaga aacaaaatca
     7741 tctttttccc aaatgcgctg tccttttttc ttgccatggg atccctcacc ttccccacaa
     7801 ctattatgcc ataacgtttc ctgcttgaaa cccgtcgaat tgcatcattg tccggattcg
     7861 ctctccctgt taggctaaaa tatgaaaaca gggggtcgat gcagatatga tttcaaaaaa
     7921 gatagggtcc atggcaatga tcgctctttc agtggccgct ctggtcggtc tctggagcat
     7981 tgggatcgcc accaacgcaa tggcggctcc ttcctggctg caggtgggac agccggttcc
     8041 agaccagttc actttcagcg gggagatccc ctcgatcgaa gaggtcaggg atgctttcgt
     8101 ccgtgtctgg ggcaacgacc ccgaaaagtg gccggaagag ttccgccgca tacacgagat
     8161 ccaggtggct ttcaacaaaa gggcaatgtc gagcagcaag ttcaaggagc aattcggcag
     8221 ggatgccctt gagaagatta aagtccagag ggaggaccct gtcggatact gggtgcgctc
     8281 cctctatgac ggctggggtt tcgaggagtg gttcaacagc gaacacctga ccgtccagag
     8341 agtcccgaga gctttgcttg tccgttttct ggaagagctg ttccccctta tgtggcggga
     8401 catccgcagt tcggtccatg tcctggagtt catgctcggc ctcccggact caaaattcat
     8461 tccctacaac aattacgact cccaggtgaa tgatctgcgc aagcgcgacg ttctggaata
     8521 caaccagtcg atagagaaaa ctgcggagag gtatataagg aataacgatt accttatgct
     8581 tcttctgatg ccggacagga tggacaccaa ctgggtcggc gacgccgggt tcgaggcatc
     8641 actggtaagc agggcgagga aactctcggc cagggtccag gaagcggtaa agaacatggg
     8701 agaccgtttc agggatgctt tttacgaggc ggttccgagg atgacctatt cctggaacga
     8761 ggaaccctgg tcagagaacc acgtcgtgat aggaggggcc ccgtccaggg tctcggccaa
     8821 cgtggatctt cttggcaaga gcacccgcat agaacacccc taccttggag acaaaaagac
     8881 gataaaggaa cagaaagtcg caagtggtgg agccatgaaa ctccagtggg gcactgctcc
     8941 agttcattcg tcggagtttg acaggggagc cgttgtggaa aaaatttccc caaggtacct
     9001 tatcgtcagg aaacaatacg gtgaatccag cgagtttgtt ctctcccttt acctggatca
     9061 tatttccggt tttggtcacg aagtcgcctt cacgcccgct gaagggccct cgttcccgga
     9121 tgagatcggg aaagaaatga tggaagctgt caccgcggcc ttttttgagg ctgccgagat
     9181 cctctacggg gaatcgtcag gaaggacgac acttgccaca aaggatacga aaagtgtttc
     9241 cccacccagg gccgaacctg gagagcagag caggataaaa aagattgtca ttgatcctcc
     9301 gaaaagcatg gagataattg ccagcggaaa aaacaatgaa ggaacccatt tcaatgtcga
     9361 ggtctgggat gagagggaga ggatcccgca ggtggaagtc tcggtcaaaa aacctgtcat
     9421 aggtacactt tcttccagga acgctaccgg tggggacgaa aaacggctta tcgtcaaaac
     9481 cggacctctc ggttatgccg agattacgta cactcctccg tcgatcccgg agatgaagtt
     9541 gctcggcata atacagaagg atatcaggat tgtggcggaa gaccccagaa gcggtgttac
     9601 atcttccatg tcactgaggg tttccaggcc gaaagacata gacatatcgc tggagcactc
     9661 tgttttgccc gctgaccctg ctttcacgaa tgctgtggat ttccgttttg atggatggga
     9721 acaaggcggg gacccggaag agaaatacag ggtcaggatc agggccctca gcgggaaagg
     9781 ggtcctttct ccgggtggtg atccgtcagg gggcaggagc tccctcgtcc tggagctgaa
     9841 agccgaccag cagcacaggc ttttttacag gtggatgggt gaaggcacca aaggaggtcc
     9901 cgtgccggag accctggttt ttgaggtgcc cgagctcgag attaagggct ttgtcaactt
     9961 ctcggttgga tttgctccgg ggctttactc ctccaggcag agttattccg aggttgaaca
    10021 accggggctt tttgttccgc tccaggtcta cgtccaggat gaatatcatc ccgatcttga
    10081 catgtctgag tttttccggg ctttcgaact taccccggtt cttgatataa gccagatcgg
    10141 ttttgagcct gttgccacgg agggctccaa aagcagggag atcctcgaag acattctcaa
    10201 acatgtcaaa ggagcgaaac ttccaaaaga cgtgctttcg ctgaaacctg agtcctggtc
    10261 actcatgagg ggcagtggtt cccgctggtt catagtggga gagacagtcg gggaaggcgt
    10321 ttcacaggga aaggcttttc ccggaataat cccgtgggag tacggagact atacgttcag
    10381 gatcagcatg aacctggagg gtccttcggg ggaagctata gagcccctga gacagaccat
    10441 gacgaggata cttcatgttc gacccttcga atccggtgtt tccgagggac aggccgagat
    10501 gatcctgccg atgatactgt ccttttctgc catgtttcca ggggaggacg cacagatttt
    10561 cgcggcccgt ggaagaaacc tccttcagag aggtgaattc gagacagcgg cggtagtgtt
    10621 gggagaaaac ttttcgcaac ggctttcatg gcagcccttt cctgaccagc cagcatcttt
    10681 ggaaaaagaa aggttaagac aacttgtctc ccaggcccac ggtgttaccg gtgctgctgt
    10741 tccggaggaa actgtagggg actctctttc tctggcgaag cagaacttcc tttgcaccgt
    10801 tgcgggggtc tacgccgaga acttcctgtc ctggggctat gacctctcgt tgctgataga
    10861 ttcaccccag atcggaaccg acaggcccga actgaggctt cttgaaatga tcaaggggtt
    10921 cctcgaagga tacggtgagt acggcatcgt ggcgatcacg agaaagaaca tcgagagctt
    10981 ttccgtttat acagaaacag gagaaagact cagggagttt ggcgggcagg tgttcggggg
    11041 aggaagccag tacgaaagga tcttttgcgg cgaacactct atagtggttc ccttcaggct
    11101 cggcgaaaat ctggttgtga ccttcaaggg taccggagag cccgttgagg cgatcaaaat
    11161 cctgcccaac gggatcaacg tccagcgtta cggcagccgc ccgggaagcg agaccataaa
    11221 cgtctatggt gatgtcgtga ggccttagag cgcgaaaagg aggggtgcgg aattgggtga
    11281 aaaaatcctg aaagtggtca accgggacag gtgcactggc tgtttcagct gtatgtatgc
    11341 ctgctcaagg cacgtgaagg gtgtcggggg aacaggcaag tcggccatga gggtccgcgc
    11401 ctactccggc accgaggggg ctttctcgat ccgggtctgt atgaggtgta ttcatcccga
    11461 ctgtgcggca gcatgtccga caggggcgct tatccccgct cccggcgggg gagtgcgcct
    11521 cgacagagaa aaatgcgtct cctgcggggc ctgtgtaagg gcctgcacca tagcggccct
    11581 tcagtgggac gaagaggagc gcgtcccgat cccctgcata ttctgcggcc agtgcgtggc
    11641 ctattgcccc aacggggtca tagcactggt tgagcgcaca tccggggggg aatagacatg
    11701 cttcagggca ggatacttca cgtcgatctt acctatggca agacacacat agaagaagag
    11761 ccggaactct tcgcgaagca tctcgggggg acagccgtgg cgacggaact actggaccgc
    11821 ttcggccgca cagatctgga cccactcgat ccccgtgccc cggtcatact tgccattggt
    11881 cccttcagtg ccctgtaccc cgtggcgaca aagaccgttt cggtctttcg gtcgcccctc
    11941 acgggagaac tgggagaatc tcatgcggga ggaaggctgg cggccgcttt aagggaaaca
    12001 ggggtcgatg ccgtggttat cagaggtgct tcaaaggatc cagtctttct gcatgtcgac
    12061 gggcatgaga tcagtctccg ctcggcgaga accttctggg ggcagtcgtc tccggcgacg
    12121 gacaggttgc tgagggaggc actggaaaag aaggggagga agctctcttt ggtaaggatc
    12181 ggtccggccg gggagaggct ctcacccatg gcgtgcgcta ccgtcgacaa ctccaggcat
    12241 tttggccgcc tggggctggg ggctgttctg ggttcaaaaa aactcaaagc cattgccgtc
    12301 agcggaaagg gatccgtagc ggtagagaac cccaaatcct accgtgatat ctatcacgat
    12361 atctacgaaa aagtagttcg ctccgatgag atgaaaaagt accacgatct tggtacgccg
    12421 gcgaacgtcc tgaccctgaa tgctatcaat ggactgccca caagaaactt ctcccagggt
    12481 tttttcgaga acgcacaaaa aatatccggc gaggccttcc tggaagaggt caacgtccag
    12541 cacatggcgt gcgcgcactg cccctgcggc tgcatccagg tggccgagct cagggagcaa
    12601 ttcgcgccct ctcattacag caccgtgagg gtctcctacg actacgaact gatcttcgcg
    12661 gtagggactc agttgagcat atccacgccg agggagatac tcagactcat tgtttttatc
    12721 gaaaagcagg gttgggactg catgagcatg ggcaacaccc tcgcatgggc gacggaggcc
    12781 ttcctctctg gtgtgataga tgaaaataat acggcaggac taagcctgtc ctttggcgat
    12841 acgtccacat acctggaggt catgaggcgc gtaagcgagg ggcaggggga gttcttccgg
    12901 gatctcgaaa aaggcacggc cttctgttcg tccaaatacg gcggcggaga ctttgccatg
    12961 cactacggag ggctggaacc ggcgggctat ctcaccggag agaacgccgt aatgacatgg
    13021 atagccgggg taagacactc gcacctcgac gacaatggct atggtatcga ccagaagctg
    13081 atcctcgggg agatatccat ggaggaccag gtgaagatgc aggtgcggga ggcccagtgg
    13141 agaatggtgc tcaactccct ggttatctgt ctctttgccc ggggagtata cgatgcctca
    13201 acaatatcaa aaggtctgga ggctctgggg ctggactgga gccctgaaaa acttcttgat
    13261 cttggagcaa gggcactgaa ggccaagtat gcctggaaga aaaagtgcgg cttcagcccc
    13321 gaaagaatta ccatcccgga aaaaatgctt cgtgtcagga ccagcaacgg cctcatcgac
    13381 agggagagaa tgaaaaaacg gctggaactt ttcctgcgct atgctggact gccttgtccc
    13441 tgtttgtcat cggagaaaag ggcaacgacg tgatgaccct gttaccagta cctctggccc
    13501 cggtgctcca gccagccggg ggtatgtttc cccgatg
//
LOCUS       DAOTIQ010000049        13427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5497_length_13427_cov_7.508750, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000049 DAOTIQ010000000
VERSION     DAOTIQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5497_length_13427_cov_7.508750"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="P5541_06900"
     CDS             complement(<1..721)
                     /locus_tag="P5541_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRV71749.1"
                     /translation="MAMRRISRLSLSCLLELSVIALICSPVFASGGPEVQMVFGAAEI
                     SNTVTGASRPLVQGDMLQAGDVIVVEGKGLVVLEEKDGTIRVRVTGGSSLGYDGVTPE
                     GLNQYEVPDGMNWFKVTPGTELDVKTPTLVASVRGTEFGVMVADGIASVAVLSGAVET
                     RDSIGRESMQGAGSSVSVSDMDMAIEEKIEGPRREREKEKESQGKGQSTGQAEPGEED
                     SAPGKSGDAPGKSGDAPGKSGD"
     gene            complement(778..2886)
                     /locus_tag="P5541_06905"
     CDS             complement(778..2886)
                     /locus_tag="P5541_06905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRV71750.1"
                     /translation="MRFKKTRFAILILVPALFFGAIGFLRPTPVERVERQVFDRNMSS
                     ISGLCPGSNIVIIAAGERSLDSLGRWPWSRGVHARLLGRIGDARVVLLDILFPEESDP
                     VEDSVLAEVIRMTGNTVLAMHFVSDSSGKDIAVFPCVSLLSASGNIGFTNVSPDIDGL
                     FRYYIPFRKSGSSYVPSLSVAALPLVLGERPKLESSRRGNLVLHTGVSSVPVDPSGRL
                     WVRFRGEPFPEYEFVDVLEGRVPADVFRDKIVLVGATAAGIEDFLMVPLPEGIEAIPG
                     VRFHAEALNTLLQGGVPVRADPLLDGFAAFILSLAGSLAAWHFRPRKGMILAALLLFF
                     MLVSTRLIFVYHGWWISLITPVISLAISFSALLYVRFRILHRDWEIKTLSIGSIYRLM
                     LTGMDSGADFGEYLGKIWPSVERDLGMKLLAPSMTPAEAVAATGVESGSKMKPGEILV
                     SRSARQKPVNRMLVQTMRDSGTGNPSYTLVGWDSDIPEEQVKSVAVWIMSSSWFYGML
                     EESERNRRLLLDTIKAMIAALDAKDPATAGHSERVAELSSSVAKKLGLDDGLVDAVYL
                     GSVIHDVGKIGIPDSILGKEGPLSDEEYRVVREHPSIGGRILETVSLPEAALRAVRNH
                     HERLDGSGYPQGLSGGEIDMASRIVSAADVFDALLSDRPYRKSWTVKEACAHMKALAG
                     KEFDEAVVEALLEVVAEEAV"
     gene            2987..3406
                     /locus_tag="P5541_06910"
     CDS             2987..3406
                     /locus_tag="P5541_06910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRV71751.1"
                     /translation="MIKLEGSKGCFVCDRTGTNARSLGVTLYWDEDSKRTVIPFTPKT
                     DWCGYEGIVHGGIITALCDDVMAWSARQASGEWSVTANISVRFLRPVKSGESYTASGS
                     ASAVEGRKIRTEARITNEAGKICADAKAVFVAVKNKA"
     gene            3526..3948
                     /locus_tag="P5541_06915"
     CDS             3526..3948
                     /locus_tag="P5541_06915"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HRV71752.1"
                     /translation="MEASTIRIRHYAPEGAACKPVQETFDTIKSLVEQLGPKLEKMGF
                     PVTLESVSSGNDTGKHNMVTMECMGVEFPETPLDEILGFEASVKPCESAAGECRVIVV
                     GDESYQAVPAGLIADGLLRVALSSTGGCGSGCGTCGGG"
     gene            4012..4533
                     /locus_tag="P5541_06920"
     CDS             4012..4533
                     /locus_tag="P5541_06920"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV71753.1"
                     /translation="MTEPWITTKGGDKGTTSLGDGSRVPKDHPRVNLYGTIDECQAAI
                     GLARASCCYEDIRQVLFEIEETMGSVMGFLALFPGIPEPDPAQLDTIVEKVRGIIGET
                     FRFVRPGDSVPGAALHMARTIARRGERLAVAMYRNGEIDETPYAWINRLSDAIYALSL
                     WTDKANRECENKN"
     gene            4612..5295
                     /locus_tag="P5541_06925"
     CDS             4612..5295
                     /locus_tag="P5541_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV71754.1"
                     /translation="MLNDPLSPARNVDLRQIVYEKIKEAIVEGLIRPGERLSEVDLAD
                     KLAVSRTPVREAIRQLAQTGLVTLEPRRGAFVSLPTVKDASDLYDLRTHLEIIAVRLV
                     TKNQPVDELERWRPVFSAMTDLTDTNHYLAEDRKFHATLYETAGNRYLTMMLHNIADL
                     INLCRHYSVEGIPLSTFAHEHISIIDAIETGDPDMAEEELRAHLERSRGGLLNYLREH
                     PELTATGEG"
     gene            5305..6189
                     /locus_tag="P5541_06930"
     CDS             5305..6189
                     /locus_tag="P5541_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV71755.1"
                     /translation="MNDTQWLGYTLAFLAAVSWAVAPVLYRRGVDHVSYAGLGAIRCS
                     GYLVSAALFLLFTMGPGAFAIPDAKTLGFIFLCSTIWLVLGDFFYFSALHKLGVSICV
                     PITSAYPLLVVPASWIFLGERVNPAVFGAAVLIVGGLVLLSPKEKSEEGRRQIRSGLL
                     VAFLAMSCWTFGILSNKILMGMIPVPQLEWWRAISVTVGSWIMYMAVDRARGLGNISF
                     RAFVEMGVAGALGLTIGNLLFTYSLSYTPVDIVACIASVRPFLAAIFATFVLRERLTF
                     RIAGGIVLVVGGVMAITL"
     gene            complement(6070..7470)
                     /locus_tag="P5541_06935"
     CDS             complement(6070..7470)
                     /locus_tag="P5541_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRV71756.1"
                     /translation="MNDERTLWDDPHTGQGVPPSKWETPLADRMRPSDLSEIAGQGHL
                     LAEDAPLARTLASGRVPNCILYGPPGVGKTSMARLMASVTGREFLEINAVSANIAALR
                     DLVSESARLRKMSGRTPVAFVDEIYHFNRQQQNALLPSVEKGELVLMGTTTENPWFEI
                     NKTLLSRLVIYELRPLSVDDLVLLMKRAIADRERGLGDLELTASPETLSELALGSGGD
                     ARQVLTRLELAALAVAAGGGSVIDPETVRKAAPRALQRYDRDQDDHHRIISALIKSMR
                     GSDPDAAVYWLARLVAGGEDIRFISRRLMIFAAEDVGLADPRAISIAASASWAADMVG
                     LPEARIILSEAVIYLAAAPKSNSAYLAVNSALSAIEKGDIQEVPGHLRPGGEGYRYPH
                     DSPGHWVKQEYMERKRRFYYPQDLGEEAKMARRLASKGDRHDPPYDQHDAAGYSEREP
                     LPEDESREDRGQKRSD"
     gene            complement(7467..8441)
                     /locus_tag="P5541_06940"
     CDS             complement(7467..8441)
                     /locus_tag="P5541_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRV71757.1"
                     /translation="MNISPPFWHPALSKGFILDWDGVLADTQLDFSPIRARYFNGERL
                     PLVEAAGRLKASEREALERDLYDLEMEGARKATPVRGSHELLEWLVSSGIPWAVVSRN
                     CRDSIFEAADRCGIELPGTVLSRDEGPLKPDPRALWAAAGSIKVDPGNCVMIGDFIYD
                     LYGAIRAGMRAVLVERTEPGWERWADISFGRLEDMVSSLRDPEPLVTREYRELVGEKG
                     RTWLDKAWGIEVSVPEESPEVFLVAMEAARLGIGRFGISGHSRISLEQWEEAPFLPIS
                     AVDSPMAECMSRTLRERYPQVSVSWSDYPRSLPEDPSLVERAIRSWLK"
     gene            complement(8513..8690)
                     /gene="ssrS"
                     /locus_tag="P5541_06945"
     ncRNA           complement(8513..8690)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5541_06945"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(8744..10123)
                     /locus_tag="P5541_06950"
     CDS             complement(8744..10123)
                     /locus_tag="P5541_06950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV71758.1"
                     /translation="MSKVCEWMRSNDLQGICGEILSFIPMPLMAGRVLEDDLIVEEVN
                     DNLRSFLHDSIMDLPESAETLFSSISDPKDMSPLDEAARGKGWSRRRWQVVKGECELE
                     LFCWGDGGDKFVAFFDDMTPHEVHTRLMTMKERILFLINLYDAKGMIPQLLSELVEHL
                     GVDWAGLFVWDGARENWVLSGDEANPAEFRQESFRNAVTFSRRLSEARGGPFGHQFGG
                     LPIQDGSLWWIPWAEDAGEWEMLMSEAGFGSLFAGVLLTGSSPAVLLCLSRESGCFAR
                     INRDVLESLWPIFLSLAERNRAVSGITKLYTRDPVTGLYAQNMMYRIMKQEMNRSRRY
                     RYPLTLIMMKIANMNVIRSRGGIEAVDETVQRVSRHALKSLRNVDIGGRIGDDTLLLL
                     LPHTPAEGANIVSGRLASNAPGLSPFPAVPLEVEIKSAVMDESCMGPEDLLAKLGLSW
                     SADAMSSKG"
     gene            10221..11006
                     /locus_tag="P5541_06955"
     CDS             10221..11006
                     /locus_tag="P5541_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV71759.1"
                     /translation="MKIPVESITAKGTFGDGEVPVHIFRPRAQNPGSPLVVLLHGVHG
                     CAGPEPGNKYGELARMLSSAGITCAVTETSRTRRDRETFGTDREGWARAAFTGKTFLE
                     DHADAIAGTEAAAAAVGTNNIWVFGFSLGGIHTVLASGEKGGFSFKPSGIALGGVGSI
                     INPEASSSLSLPILETVPPEKTLIEAAARLRTGKVVSFYGSLDATFPEDSCRRLVEAV
                     PLPCERKRFIVMEGVDHAFRSIRGIPSIKPLEMISRVMALSVF"
     gene            complement(11003..11785)
                     /locus_tag="P5541_06960"
     CDS             complement(11003..11785)
                     /locus_tag="P5541_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRV71760.1"
                     /translation="MSFAVTVIFISFSALLGASMGSFLNVVASRTVEDRPWWGGERST
                     CDSCGKELSPGELVPLLSWVFLSGKCRSCGAKIPLRYFGVEAAGAVTGALIAWRWGFS
                     PTAAFASVVACGLFLNALTDLYSGYVYDLFAWGLGAAGLLMRVGGGLNGLIDGLLGAV
                     LGFAVIAAIIILSRGGMGWGDASLMAGTGAALGWKLTAWGLYLGFMTGGVVAVSLLVL
                     KLVKRKDAIPLGPFLALGGLLSMLSGPWFFRFLGMTLSWPWS"
     gene            complement(11863..12306)
                     /locus_tag="P5541_06965"
     CDS             complement(11863..12306)
                     /locus_tag="P5541_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71761.1"
                     /translation="MQMPMQISLDEILSMLVARVDSLSFNEENLKTKFNILGRAMYKK
                     GLISDEDIEDSIREEHRILKDLGVIQEMPADDVIEAISQGILQWIKGDVEAIKKAMEE
                     YEKKVEEASKKQAGKPKIDVASPAVLQQLDKMSGKNPRGGGKLIL"
     gene            12421..13302
                     /locus_tag="P5541_06970"
     CDS             12421..13302
                     /locus_tag="P5541_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRV71762.1"
                     /translation="MRIRKKIVHATVAVRREIPTFALMTFGATIVAFGIMSLTIPYRF
                     PDSGVSGIAVLSSYLFGISPAWIVGIANLVLLAWGLKELSPRFIGWTVYAVMLLTLLL
                     KLFEGIPYPHLDERLLVAILAGVVKGLGGGLVLRSGSSLGGTDIIVVVLRKRYGVEVG
                     KFTFYINLAILGISVFIVGLEGAIYGLVSIFANGLVTDNVLRSFDRRRQVIVISNTPR
                     EIAAFITGEMHRGVTILHGEGGYTHEERPALLCVLTPRQTMDLKNYLSVHDPKAFMIV
                     SDASEVMGRGFKNWTEL"
BASE COUNT         3049 a         3987 c         3584 g         2807 t
ORIGIN      
        1 catcgccaga tttaccgggg gcatcaccgg attttcccgg agcatcgcca gatttccctg
       61 gagcggagtc ttcttcaccc ggctcagcct gccccgttga ctggcccttt ccctgggact
      121 ctttttcttt ttctctttca cgtctgggcc cttcgatctt ttcctcgatg gccatatcca
      181 tgtcggatac gctgaccgat gaccctgctc cctgcatgga ttcccgccct atggagtcac
      241 gggtctccac ggctcctgaa aggaccgcca cagaagctat gccatcggcc accatcacgc
      301 cgaactcggt tcccctgacc gatgcaacca gcgtaggagt cttgacatca agttcggtcc
      361 cgggagtcac cttgaaccag ttcatgccat ctggaacctc gtactggttc aatccttccg
      421 gagtcacacc atcataaccg agggaagatc cgccggtaac ccggacacgt atggtaccat
      481 ccttttcctc cagcacgaca agaccctttc cttcaaccac gatcacgtca ccggcctgga
      541 gcatatctcc ctggaccagt ggcctggaag cccccgtaac ggtattgctt atctccgccg
      601 ctccaaagac catctgaacc tcgggccccc cggaggcaaa aaccggcgag cagatcaaag
      661 caataacgga aagttcaagg agacaactta aagataacct tgaaatcctc ctcatggcca
      721 tcaattccct tcccttgagg taactttgta cgcttggttg aaatcactat tattatatca
      781 gactgcctct tcggcaacta cctcgagaag ggcctcgacc accgcttcgt caaactcttt
      841 tccggcgagg gccttcatgt gggcacaggc ttccttcact gtccaggact tcctgtatgg
      901 cctgtcgctg agcagggcat caaaaacatc cgccgccgag actatccgcg aggccatatc
      961 tatctccccg cccgaaaggc cctgcggata acccgacccg tcaagcctct cgtgatggtt
     1021 tcttacggct ctcagcgcgg cttcgggaag agatacggtt tccagtatcc tgccccctat
     1081 tgatggatgc tccctcacaa ccctgtactc ttcatcggag aggggaccct ccttgccgag
     1141 gatgctgtcc gggataccga tcttcccgac atcgtgtatc acagatccaa ggtagacggc
     1201 atccaccaga ccatcgtcca gaccaagttt ttttgccaca ctggatgaca gttccgccac
     1261 cctttcggag tgtcccgccg tcgccggatc tttggcatcg agtgccgcga tcatggcctt
     1321 tatggtatct aacagaaggc gcctgtttcg ctcactctcc tcgagcattc cgtaaaacca
     1381 cgacgaggac attatccata cagccacgct tttgacctgc tcctccggga tatcactatc
     1441 ccagcctacg agagtgtaag acggatttcc ggttccggag tctcgcatag tctggacaag
     1501 catcctgttg accggtttct gccttgcgct tcttgagaca agtatttctc ccggtttcat
     1561 cttgctgccg ctttcaaccc ccgtcgccgc aacggcttcg gcaggagtca ttgatggcgc
     1621 cagcagtttc atgccaaggt cgcgttcgac agatggccag atcttcccca ggtattctcc
     1681 gaaatcggcc cccgaatcca tccccgtcag cataagcctg tagattgatc caattgaaag
     1741 agtttttatc tcccagtccc tgtggaggat cctgaagcgc acgtataaca gggcagagaa
     1801 tgaaatagca agggagataa cgggagttat cagtgatatc caccatccat ggtaaacaaa
     1861 gatcaggcgg gtagagacca gcataaagaa gagcaacagc gccgccagga tcattccctt
     1921 cctcggacga aaatgccatg ccgcaagaga tccggcaagg gagaggataa aagcggcgaa
     1981 gccgtccaga agagggtcgg ccctgaccgg cacgcctccc tgaagaaggg tgttaagggc
     2041 ctccgcatgg aacctgacac ccgggatggc ctctattcct tccggcaatg gaaccatgag
     2101 aaaatcttca attcccgctg ccgtggcacc tacgagcacg atcttgtccc tgaagacatc
     2161 cgccgggaca cgcccctcca gcacatcgac aaattcgtat tcgggaaatg gctccccgcg
     2221 aaatctcacc cagagccttc ctgaaggatc aacagggaca gaagagaccc ccgtatgaag
     2281 aacgaggttg cctcttctgg aactttcgag tttcggcctc tctccaagca caagggggag
     2341 cgcggcaacc gagagggagg ggacatagga tgatccgctc tttctgaaag ggatgtagta
     2401 acggaacagg ccgtcgatat ccggcgatac gttcgtgaaa cctatgttcc cggaagccga
     2461 gaggagcgaa acgcatggaa agacggctat atccttaccg gagctgtctg acacgaaatg
     2521 catcgcaagg actgtgttgc ccgtcatgcg gataacctct gccaggacgg aatcctcgac
     2581 ggggtcactt tcttcgggaa aaaggatgtc gagcagcacc accctggcat caccgatacg
     2641 gccaagaagc cgggcatgta cccctctgct ccagggccat ctcccgagac tgtccaggct
     2701 cctctccccc gccgcgatta ttacgatatt gctcccgggg cacaatcccg aaatggaaga
     2761 catgttcctg tcgaaaacct gcctctccac cctttcaacc ggagtcgggc gcaaaaaccc
     2821 gatggcaccg aaaaaaagag cggggacaag tatcaaaatc gcgaaacggg tttttttgaa
     2881 ccgcaaacgc atcccctcag atcaaaggtc gaatttgaat tcattaagta tatcccttta
     2941 agtgcgttta taatataatc cgcgaaggcg gtgatagtta gtggccatga tcaaacttga
     3001 aggttctaag gggtgctttg tctgcgacag aacaggcacc aacgcccgct cgctgggagt
     3061 caccctctac tgggatgagg attcaaaaag gaccgttatc cctttcactc ccaagaccga
     3121 ctggtgcggc tatgaaggaa tagtccacgg gggtataata acagctcttt gcgacgacgt
     3181 tatggcatgg tcagcaaggc aggcatccgg agagtggtcg gtaacggcaa atatctccgt
     3241 tcgttttctg cggccggtaa aatcggggga aagttacact gcatcgggaa gcgcctcggc
     3301 agtagagggg cggaaaatcc gcaccgaggc aaggatcaca aatgaagctg gcaagatctg
     3361 cgcagacgcc aaggcggtgt ttgtggcagt aaaaaacaag gcgtaaacag taaatagtga
     3421 acagtaaacg gtaatggcaa ggaacaagat caggatattt tttgaagaag tttaccgttc
     3481 accgtttacc attcacggtt tacgaatatc agggaggtaa caataatgga agcatcaacg
     3541 atcaggatca ggcactacgc acctgaagga gctgcgtgca aacctgtgca ggagactttc
     3601 gataccatca agtctcttgt ggagcagctt ggaccaaagc tggaaaaaat gggtttccct
     3661 gtaacgctcg aatcagtttc gtccggaaac gacaccggaa agcacaacat ggtcaccatg
     3721 gaatgcatgg gggtcgaatt cccggaaact ccgctcgatg agattctcgg ctttgaggcc
     3781 tcagtgaaac cctgcgaatc tgcagccgga gaatgccgcg ttatcgttgt cggggatgag
     3841 tcctaccagg cagttcccgc cggccttatc gcagacggcc tgctgagggt agcgctttca
     3901 tccacagggg gctgcggaag cggctgcgga acctgcggcg gcggctgagg catctaattt
     3961 acggctgcag tctgcagcca actcaaaatc cagatccacg gaggctcttt catgacagaa
     4021 ccctggatca ccaccaaggg cggtgacaag ggaaccacca gtctgggcga tggatctcgt
     4081 gtgccaaaag accacccgag ggtgaatctt tacggaacca ttgatgaatg ccaggcagcg
     4141 ataggcctcg ccagggcttc ctgctgctac gaggatatcc gccaggttct tttcgagata
     4201 gaggagacca tgggctcggt catgggattc ctggcactat ttccgggtat ccccgaacca
     4261 gatccggcac aactggatac cattgtggaa aaggtccgcg gaatcatagg ggaaaccttc
     4321 cggttcgtac gccccgggga ttcggttccc ggtgcggccc tgcacatggc tcgaacaata
     4381 gcccgtcgtg gcgaaaggct ggcagtggcc atgtacagaa acggggagat agacgaaact
     4441 ccctacgcct ggatcaaccg tctctccgac gcgatctacg ccctttccct ctggaccgac
     4501 aaggcaaacc gcgaatgtga aaacaaaaac taactggtgc tggctttttg aaaaatgtcg
     4561 agtgtataat ggacagagta tacgctcgac attttttcgg gaggcggtct catgttgaat
     4621 gatccactat ctccggcgcg caatgtcgac ctccggcaga tcgtctatga gaagatcaag
     4681 gaagccatag ttgagggtct cattcgcccg ggagaaaggc tttcggaggt agacctcgcg
     4741 gacaaactgg cagtctccag gacccccgtc cgggaggcca tcaggcaact ggcccagact
     4801 ggtctggtaa ccctggaacc acgccggggg gcttttgtgt cacttcccac ggtcaaggac
     4861 gcgtcagacc tttacgacct caggacccat ctggaaataa tcgcggtgcg cctggtcaca
     4921 aagaaccagc ctgtcgacga actcgaaagg tggagacccg tcttttcggc catgacggat
     4981 ctgaccgaca ccaaccacta cctggccgag gacagaaagt ttcacgccac cctgtacgag
     5041 accgccggga accgctacct tacaatgatg ctccacaaca tagcggacct cataaacctt
     5101 tgcaggcatt attcggtgga ggggatcccc ctgagcacct tcgcccacga gcatatctcc
     5161 attatcgatg ccatcgagac gggcgacccc gacatggcgg aagaagaact tcgcgcccac
     5221 ctcgaaagat ccaggggcgg acttctcaac tacctgagag aacacccgga actgacagcg
     5281 accggtgaag gttaagtccg ggacatgaac gatacccagt ggctgggtta taccctggct
     5341 tttttggccg ctgtttcctg ggcagtggct cccgtactct accggcgagg ggtggatcat
     5401 gtttcctatg cgggtctggg agccataaga tgttcgggat acctcgtcag tgcggccctt
     5461 ttcctgctct tcaccatggg acccggggca ttcgccatcc ctgacgcaaa gactttgggg
     5521 tttatctttt tgtgctccac catctggctt gtcctgggcg acttcttcta tttttcggct
     5581 ctccacaaac tgggggtctc tatatgcgtc cccataacct cggcctaccc cctgctggtg
     5641 gtcccggcct cgtggatatt cctcggagag agggtcaacc ccgccgtttt cggcgctgca
     5701 gtccttatcg tcggagggct tgttctcctt tcaccgaaag aaaaaagcga ggaaggaaga
     5761 cgccagataa ggagcgggct tctggtggcg ttccttgcga tgtcctgctg gacctttggc
     5821 atcctcagca acaaaatact tatgggaatg atccctgttc cgcagctgga gtggtggcgc
     5881 gccataagtg ttaccgtcgg atcctggatc atgtacatgg ccgtagacag ggcaagggga
     5941 ctggggaata tctccttcag ggcctttgta gaaatggggg tcgccggggc gcttggcctg
     6001 accatcggca atcttctctt cacctacagt ctctcttata cacccgtgga catagtggcg
     6061 tgtatcgcct cagtcagacc ttttctggcc gcgatcttcg cgactttcgt cctcagggag
     6121 aggctcacgt tccgaatagc cggcggcatc gtgctggtcg tagggggggt catggcgatc
     6181 accctttgag gccagccgcc ttgccatctt cgcttcctcg cccagatcct gaggatagta
     6241 aaaccgcctt tttctctcca tatactcctg ctttacccag tgcccggggc tgtcatgggg
     6301 gtatctgtac ccctcgcctc cgggcctgag gtgcccggga acctcctgaa tatcaccttt
     6361 ttcgatggcc gacagggcgc tgttcactgc caggtaggca ctgttgcttt tcggtgccgc
     6421 ggcaagatag atcaccgctt ccgataatat gatcctggcc tcgggaagcc ccaccatgtc
     6481 cgcggcccac gaagcggatg cagctatgct tatcgcccgc gggtcggcga gccctacatc
     6541 ctcggccgcg aatatcatca accgtcttga aatgaaacgg atatcttctc cccctgccac
     6601 gagcctggca agccagtata ccgccgcgtc cgggtcggaa ccacgcatgc tcttgatcag
     6661 tgcggaaatg atcctgtggt gatcatcctg gtcgcggtcg tatcgctgca gggctctggg
     6721 cgccgccttt cggaccgttt cggggtcgat cacggatcct ccgcccgccg cgaccgccag
     6781 ggccgccagt tccagccggg tcaggacctg tcttgcatcg cccccggaac caagggccag
     6841 ctcactcaat gtttcgggag aggcggtcaa ttccaggtct ccgagacctc tttccctgtc
     6901 ggctatcgcc cttttcatca gcagcaccag gtcgtcaacg gatagaggcc ggagttcgta
     6961 gatcacgagg cgcgacagaa gggtcttgtt tatctcgaac caggggttct ccgtggtagt
     7021 ccccataaga acaagttcgc ccttctccac cgagggaaga agggcattct gctgttgcct
     7081 gttgaagtga tagatctcgt ccacgaaagc gaccggcgtc ctgccgctca tttttctgag
     7141 tcttgcggat tcggatacaa ggtcacggag agctgctatg ttggccgata ccgcgttgat
     7201 ctccaggaat tccctgcccg tgacggatgc catcagccgc gccatcgatg tctttcccac
     7261 tcccggcggg ccgtagagga tgcagtttgg cacacggccc gaagcgagag tcctggcaag
     7321 gggcgcatcc tccgccagca ggtgtccctg tccggctatt tccgaaagat ccgaagggcg
     7381 catccggtcg gcaaggggag tttcccactt gctcggcgga actccctgcc cggtatgtgg
     7441 atcatcccaa agggtccttt catcgttcat ttcagccatg atctgatcgc tctttccaca
     7501 agcgaaggat cctccggcag ggatcgggga tagtcgctcc acgagacaga gacctgcggg
     7561 tatctctccc gaagggttcg agacatacac tccgccatgg gagagtcaac cgccgagatg
     7621 ggaaggaacg gagcctcctc ccactgctcc agggagatac gggaatggcc cgatatcccg
     7681 aacctgccga tcccgaggcg ggcagcctcc atggccacca ggaacacttc gggagattct
     7741 tccggaacgg agacctctat cccccaggcc ttatccagcc atgtcctgcc cttttccccg
     7801 accagttcac ggtactccct cgtaacaagg ggttccggat ctctcagaga agataccata
     7861 tcctccagcc tgccgaagga tatgtcggcc catctctccc agccaggctc ggtccgctcg
     7921 accaggaccg ctctcatccc cgccctgatc gcgccgtaga gatcgtagat gaaatcccct
     7981 atcatcacac agttgccagg gtccactttt atcgaaccgg cagcggccca gagcgctcgg
     8041 gggtcgggtt tgagaggtcc ctcgtccctg gagaggaccg tgccggggag ttcgataccg
     8101 catctgtccg cggcctcgaa tatcgagtcc ctgcaattcc tcgagaccac cgcccagggg
     8161 ataccggaag aaaccagcca ctccaggagt tcatgagagc ccctgacggg tgttgccttt
     8221 cgggctcctt ccatctccag gtcgtagaga tcgcgttcca gggcctcccg ctcagaagct
     8281 ttgagacgtc ccgcggcctc caccaggggc agcctctcgc cgttgaagta ccgggccctt
     8341 ataggcgaaa aatccagttg cgtatccgca agaaccccgt cccagtccag aatgaaacct
     8401 ttggagagag ccgggtgcca gaatggggga gaaatattca aaaatccacc tccgaataaa
     8461 gaaaaagcgg ggcatggcaa gacccgaagc cgccattccc cgctttataa aatgcgcccc
     8521 cgcgagtgcc gtgacacgaa ggccttgacc cgatcctaaa aagtggaagc ctcacccgcc
     8581 tcaactccgg cagcgccttg acgccgccat cgccggcagg tagaacagcc tcccataacc
     8641 gggtgttggc tcaaggcact tcaatatgtt cacgaacacc gcagggcctg ttctcttttg
     8701 tcgtctacga agtcattata gcacaatggg gcaagactat tctctaaccc ttactggaca
     8761 tggcatcggc agaccacgaa aggccgagtt ttgccagcag atcctcgggc cccatgcatg
     8821 attcgtccat tacggctgat ttgatctcga cctccagggg aaccgccgga aaaggcgaaa
     8881 ggccgggcgc gttggaggca agccttccgg aaacgatatt ggccccttcg gcaggcgtgt
     8941 gaggaagaag caggagaaga gtgtcatccc cgatcctgcc cccaatgtca acgttccgca
     9001 gacttttcag ggcatgcctc gagactctct gaaccgtctc gtcgacggcc tctatgccgc
     9061 ccctggaacg aataacgttc atgttggcta tcttcatcat tatgagggtc agcggatacc
     9121 gataacgcct ggagcggttc atctcctgtt tcattatccg gtacatcatg ttctgggcgt
     9181 aaaggcctgt gacgggatct ctcgtgtaca gtttcgtgat gcctgaaacg gcgcggttcc
     9241 tctcggccag ggaaaggaat attggccaca gagactccag aacatccctg ttgatcctgg
     9301 caaagcaccc cgattcccgg gaaagacaaa gaagcaccgc ggggcttgac cctgtcagaa
     9361 gaacaccggc aaacagtgac ccgaagccag cctcggacat gagcatttcc cactccccgg
     9421 catcttctgc ccagggtatc caccagagag aaccatcctg tatgggcagc ccgccgaact
     9481 ggtggccaaa tggcccgccc cgtgcctcgg acagcctgcg ggagaaggtc acagcgttgc
     9541 ggaaggactc ctgcctgaac tctgcagggt tggcctcgtc accagagagg acccagttct
     9601 cccgcgcccc atcccatacg aaaagaccag cccagtcgac gccaaggtgc tccacaagtt
     9661 ccgacagcaa ctgggggatc ataccctttg cgtcatagag gtttatcagg aagagtatcc
     9721 tctccttcat ggtcatcagc ctcgtatgaa cctcgtgggg cgtcatgtca tcgaagaagg
     9781 cgacgaattt gtctcctccg tcgccccagc agaagagttc cagttcacac tctcccttta
     9841 caacctgcca tctcctgcgc gaccagccct tgcccctggc cgcctcgtcc aggggtgaca
     9901 tatccttcgg atcgcttatg ctggagaaaa gggtttcggc gctctcaggc aagtccataa
     9961 tggagtcgtg gagaaaactt ctcaggttgt cgttgacctc ttcgacgatc aggtcatctt
    10021 ccaggaccct gccagccatg aggggcatcg gaataaacga aaggatctca ccgcatatcc
    10081 cctggaggtc attagatctc atccactcgc aaaccttcga catgaaaggc ttccccctcg
    10141 ccggaacagg ttaaccacaa cactatgata aactgaaagg cagaactttt gcaaaatatt
    10201 gcccgaaagg ggcccgcgac ttgaaaatcc ctgttgaaag cataaccgca aaaggaactt
    10261 tcggggacgg ggaagtgccc gtccatattt tccggccccg agcccagaac ccggggtccc
    10321 cgctcgtggt gctcctccac ggtgtccatg gctgcgcagg gcccgagccg gggaacaaat
    10381 acggcgaact cgccaggatg ctttcctcag ctggcattac ctgcgccgta accgaaacca
    10441 gccggacgag acgcgacagg gagacctttg ggacagaccg ggaagggtgg gcgcgggccg
    10501 cattcaccgg caagaccttc cttgaggacc acgccgatgc catagccggg acggaagccg
    10561 ccgcggccgc tgtcggcacc aataatatct gggtcttcgg cttctccctt ggagggatac
    10621 acacggttct ggccagcgga gaaaagggcg gtttctcctt taaaccctcg gggatagcgc
    10681 tggggggggt cggatccatc atcaaccccg aagcatcttc ctcgctctct ttgcccatac
    10741 tcgaaaccgt gcctccggaa aaaaccctga ttgaggcggc agcgaggctc cggacaggta
    10801 aggtcgtatc attttacgga tcccttgacg ccacgttccc ggaagattcc tgcaggcggc
    10861 tggttgaagc ggttcccctg ccttgcgaaa gaaagaggtt catcgttatg gagggcgtcg
    10921 atcacgcctt ccgcagcata aggggcatcc cttcgatcaa acctctcgaa atgatatcca
    10981 gggttatggc gctttcggtc ttctaactcc agggccagct taaggtcatc ccgaggaacc
    11041 ggaaaaacca tggtcctgaa agcattgaaa gaagaccccc cagggcaaga aaaggtccaa
    11101 ggggtatggc atcctttctt ttcaccagtt tcagcacaag cagggaaaca gccacaacac
    11161 cgcccgtcat gaaaccaagg tagaggcccc aggcggtcag tttccaccca agggcagccc
    11221 ccgtcccggc catgaggctt gcgtcacccc atcccatccc accgcggctc aggatgatta
    11281 tcgccgctat taccgcaaaa cccagaacgg cccccagcag gccatcgatc aggccattaa
    11341 ggccgcctcc gaccctcatc agtagccctg ccgccccgag accccaggca aagaggtcgt
    11401 agacatatcc gctgtaaaga tctgtgaggg cattcagaaa aagaccacaa gctactaccg
    11461 aggcaaaggc agccgtcggg gaaaatcccc atctccaggc gataagggcc cccgtgaccg
    11521 ccccggcagc ctcaactcca aaatatctca gcgggatttt tgccccacat gaccggcatt
    11581 ttcccgaaag gaagacccag gaaagcagag ggaccagttc cccgggagaa agttccttgc
    11641 cgcaggagtc gcatgtggac cgctcgccgc cccaccacgg acgatcctcc actgtgcgcg
    11701 aggccacgac gttcaggaac gatcccatgc tggcccctag cagggccgaa aaagaaataa
    11761 atatcacagt aacagcaaaa gacatcttca acaccatccg cgacaggagt ccgtaaaaaa
    11821 ggccccggca aaaaagccgg gaccctggaa aaacatctaa aactagagta tcagtttgcc
    11881 gccacccctg gggttcttgc cgctcatctt gtcgagctgc tgcagcacag ccggagaagc
    11941 cacgtctatc ttcggcttgc cggcctgctt tttcgaggct tcctcgacct ttttctcgta
    12001 ttcctccatg gcctttttga tcgcttccac gtctcccttt atccactgaa ggatcccctg
    12061 ggaaatcgcc tcgataacgt cgtcggccgg catctcctgg attacgccaa gatccttgag
    12121 gatacggtgc tcctcgcgaa tggaatcctc gatatcctca tcggatatga gccccttctt
    12181 gtacatggcg cgccccagaa tattgaactt tgtcttcagg ttctcctcgt tgaaggagag
    12241 gctgtctact cttgccacaa gcatggacag gatctcgtcc aggcttatct gcatcggcat
    12301 ctgcatatca ggatatgccc ccttatattg agatcgggaa caattgtaac acgaccaccc
    12361 tgagggtggc ttataatcat ttaggcgaaa cttttctaaa aatcagggag gaacgggcgt
    12421 atgcggatca ggaaaaagat tgttcatgca acggttgccg tcaggagaga gatccccaca
    12481 ttcgcgctaa tgaccttcgg cgcgaccata gtggccttcg ggattatgtc tctgacaatc
    12541 ccgtaccgtt tccccgactc gggagtatcc gggatagcgg tgctctcgag ttatcttttt
    12601 ggcatttccc cggcctggat agtgggtatc gcgaaccttg tcctccttgc gtggggcctg
    12661 aaggaactat ctccccgttt tatcgggtgg acggtatacg ccgtcatgct cctgaccctc
    12721 cttctgaaac tgttcgaggg gataccctac ccacacctcg acgagaggct tctcgtggcg
    12781 atccttgcgg gcgtagtgaa ggggctggga ggggggcttg tcctccggtc gggaagttct
    12841 ctcgggggaa cggacatcat cgttgtggtc ctgcgaaaga gatacggcgt ggaggtggga
    12901 aagttcacct tctacataaa cctcgcgata ctgggaatat ccgtcttcat agtcggcctc
    12961 gagggagcca tctacggcct ggtcagcata ttcgccaacg gcctggtaac ggacaacgtg
    13021 ctgaggtcct tcgaccggag acggcaggta atcgttataa gcaacactcc tcgagaaatt
    13081 gcggctttca taactggaga aatgcaccgc ggagtgacca tacttcacgg ggaggggggc
    13141 tatacacacg aggaacgccc ggcgctttta tgcgtcctga ccccgcgtca gaccatggac
    13201 ctgaagaact acctgtccgt ccacgacccg aaagccttca tgatcgtctc cgacgcttcg
    13261 gaggtcatgg gaaggggatt caagaactgg acggaactgt aggaacaagg cgtcaggcga
    13321 ctggagacag gagacaggaa agcaaaatta aaggcgtaaa cggtcaacgg tcaacggtga
    13381 acggaaaagg cagaaggtcc tgtcattctg aggaccaacg ggacgaa
//
LOCUS       DAOTIQ010000050        13267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5589_length_13267_cov_6.292537, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000050 DAOTIQ010000000
VERSION     DAOTIQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_13267_cov_6.292537"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..782)
                     /locus_tag="P5541_06975"
     CDS             complement(63..782)
                     /locus_tag="P5541_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71763.1"
                     /translation="MSGGKGITVEGLKKRYGSGETAVDALKEVNMVVAPGEVVGLIGP
                     SGSGKTTLLKCLGAVIEPTAGKMTIGDNVIYEEKWKIKDLRALRRDKIGFVFQAPYLI
                     PFLDATDNVALLPMLAGKPNGEARDRARELLDALDVAHRARAMPSELSGGEQQRVSIA
                     RALANQPPVILADEPTAPLDSERAMAVIRILNSMAKQYEAAIIVVTHDEKIIPTFRRI
                     YRIRDGKTEEEAGEGRSTEPT"
     gene            complement(788..1990)
                     /locus_tag="P5541_06980"
     CDS             complement(788..1990)
                     /locus_tag="P5541_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71764.1"
                     /translation="MISLAGRDILHGWGKFVLTGFGLGLLIGVTLTMGGVYRGMVDDA
                     RVLLDNSGADLWVVQQDTLGPYAESSSIYDDLWRSVAGMKGVDAAANVTYLTTQIRRG
                     EEDVRAMVVGIEPGVLGGPGQPRYLVAGRHITRSHYEAVADEASGFRLGDSIGIRRNS
                     YRVVGITRRMVSSGGDPMVFIPLKDAQEAQFLKDNDFILQQRRRTEANPSLNRPGIPG
                     LLESVLASQTGNSTVNAVLVRLAPGADPEETAEHIRRWKRHEVYTREQMEEILLGKLI
                     ATSSRQIGMFLVILAIVSAAIVAFIIYTLTMGKIREIAVLKLIGTKNRTIVGMILQQA
                     LGLGVLGFMIGKISATIWAPIFPKYVLLEGRDSVAGFIAVMIMCVASSVLAIRMALKV
                     DPAEAIGG"
     gene            complement(1987..3177)
                     /locus_tag="P5541_06985"
     CDS             complement(1987..3177)
                     /locus_tag="P5541_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV71765.1"
                     /translation="MKGLKGKGKTIFLLLGFLVLAVFFFYFMMRSGPLAPVEVTATVV
                     EVRSLTPALYGIGTVNSQASYRIGPTIPGRVKDLSVRVGDLVEKGQVLGAMDPVDWEQ
                     RLAAQDLSIRRLEASLQAGEARIAEVAARGNYASELAKRYDNLLKVGAVSLDAAQAKR
                     QEAQALAALENSARAERDAIRADLARAREDRKGLEQQKDNLLLVSPAEGLVTARNVEP
                     GTTVVAGQAVVEVIDRENLWISVRFDQAMASGLTPGLPASISIRSRGDTVFPGRILRV
                     EPLADAVTEELMAKAVFDTVPDVLPPLGELAEVTVTLPPLPETAVVPDSALQRLNGDL
                     GVWVLDNGSITFRPVKTGRRDLDGFVQIMSGLEKGENIVQYMKKALSPRSRVKVVENL
                     EAKE"
     gene            complement(3174..3788)
                     /locus_tag="P5541_06990"
     CDS             complement(3174..3788)
                     /locus_tag="P5541_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV71766.1"
                     /translation="MVQKGKNLPAQERKRVIVETVISLAAEGKPSDITTAAIAERMNL
                     TQGALFRHFPTKEAIWKAVLDWVGKSLFSRLDKAAADAGSPLEALEAIFLAHVQFASE
                     HPGAPRLLLSEFQRTEESPVRSLVHSLLKKYDSRVVALLEEGKMTGDVDPALDSPFGA
                     ALFTGAIQGLVLQSQIEEDKRDGLLKAHGVFAIYRRGIERRDPA"
     gene            complement(3975..5072)
                     /locus_tag="P5541_06995"
     CDS             complement(3975..5072)
                     /locus_tag="P5541_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71767.1"
                     /translation="MNKAKGPWIHRFMIYFFTVVLAVLVFWVLGFLVEDIGAIRGPQY
                     TEIEQRYVDQDLVDRAKTLMAEITDVDRAISDKREEMRIVNDSSQNLQNTINQLIELQ
                     KLAVQRSVSLSDKEQTNLSESLAHFLESQKRYQVYNTELSRLTDRKRSLESERSAIEK
                     KIAGQSLPAGEEFRVLREKHDLRLAGYQLLILLPMLAAGAIFVVRKRGSVYFPLFLGF
                     AAATLLKTAFVIHRYFPARYVKYVLVGGLLIAVIKLLVYFIRVIAFPKADLLMKQYRE
                     GYERFFCPVCDFPIRRGPRKFLFWTRRTVGKLELPRGSDEDSPYSCPACGTVLFEECP
                     KCHNIRYSLLTFCQHCGAVKEIEHGSDNAKV"
     gene            complement(5352..7085)
                     /locus_tag="P5541_07000"
     CDS             complement(5352..7085)
                     /locus_tag="P5541_07000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRV71768.1"
                     /translation="MRSRPSVFALLLLLSLVLVHPGESCTTILVTKGASSDGSVMISH
                     SDDNDLMDQRIIRVPAQDHAQGALRPVFCTAAAIGAFPQYNSFSYPRMVTGDRGRGYD
                     TAGPASIPLGHIPQVEKTYAYFDGSYGIMNEHQLMFGECTDGSKVTLDPEPGKRIFYS
                     AELSRVALERCRTAREAIRVIGELIEKYGYYGTGETLPVGDTEEGWVMEMAPSPEGVG
                     GLWVAKKVPDGEVFVGANEFRIREVDPDDPDMMFSKDLHEIAQKHGWWKPEDGKLDWL
                     KTVSLGEYNHPYYSLRRVWRVLSLVAPSRNFTPWVEDGFTKDYPFSVKPDKRLSVRDV
                     MGLHRDHFEGTEFDLTKGVAAGPFGYPDRFYGPYDGKGDVGDPSRKLEGAWERPLSVS
                     YCGYVYVNQARGWLPDPIGGIMWLGLDKPTDTVFIPFHVGVTSLPRSVEVCNTEKFSR
                     ESAWWTFNFLSNYQGLKYSYMHKEIVVLQEQLEKGFLVFLPEVEKRALQLYEKDPSEA
                     VKYLTELAENYTASVMKEWWDLSEQLIVKYNDGLLNEPGNMGQPLGYPGEWLETTSWP
                     NGPTTYQNPES"
     gene            complement(7172..8278)
                     /locus_tag="P5541_07005"
     CDS             complement(7172..8278)
                     /locus_tag="P5541_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71769.1"
                     /translation="MSLGSLIMFPVILSLCFSFNLSTAEAYEPPVYVDYEYGDLKEVI
                     VGLPFGMTPSLSAPWFENAMKVLPPDEAEYARKTAGMLWTDMIDPKTGKSETEKLEEE
                     NLALIGILNSLGVKVYRPREITVDFIKENYGSDVLENGFSQDFPRDNMAVIGNSLIEF
                     NLRTPLRKVDISGFREILLERCTKEGVRWFSMPHTELLAPPSPDTPLLEGGDVIVLGR
                     TILVGNTLNPSVGSNEAGFRWLKNILGSSYDVVRVPLREDVLHLDCVLSVPRRGLAIV
                     CEEAFVDGLPDHIKGWDLIKVDIESVRRLTVNGIPVDGENYIMSFNEHNDNRYLQAEL
                     EKRNIKVHRVFFGTHNGQGGSLRCATQPVIRKIR"
     gene            complement(8537..8932)
                     /locus_tag="P5541_07010"
     CDS             complement(8537..8932)
                     /locus_tag="P5541_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRV71770.1"
                     /translation="MTGLFLKNYLFTLLPFVALDAVWLGLVAKKFYRSQIGYVMAERP
                     NWIAAAVFYLLFIAGMVFFVTGREGTLQEAAFRGAVFGLICYATYNLTNLATIRRWPV
                     IVTVVDMTWGTILGGSTAFIAVWLKGLFS"
     gene            complement(9506..11020)
                     /locus_tag="P5541_07015"
     CDS             complement(9506..11020)
                     /locus_tag="P5541_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV71771.1"
                     /translation="MSISFQHVSFAYPGNASPIFGDVTVHFAEGWTGIVGANGAGKTT
                     FLQLAMGQIEAQTGTIKRKGSMVFCPQRTDDMPENLSRFVSATDAEAWVLRGELQVEE
                     NWATRWPTLSHGERKRAQIGVALWQRPDVLLLDEPTNHIDLIARELLVATLSSFNGIG
                     LLVSHDRDLLDLLCQRCIFLDPPEVVVRPGNYSKAVSDAQREESSLIARREILQQNLE
                     RLKIESRRRYEKASRAGRRSSKRNLARGDSDGRARIDLARVSGQDGQAGRLARQPESR
                     IARLHEKISGIKIKKRYDTYFWLEGSVSPRKCLFSIAGEIIDLDGQRRLKVPEFSIIR
                     DDRIAITGENGLGKSTFVRFILDSMDLPGERLVFLPQEIDLAQTRKIMEDVHRLSREQ
                     LGTVMTVVSALGSRPERLLNNLDASPGELRKVLLALGVIRHPYLIVMDEPTNHLDIPA
                     IECLEDALRVCPCALLLISHDLRFLSRVARKRWHLQQHGREITFAMKELERTGY"
     gene            complement(11139..11729)
                     /locus_tag="P5541_07020"
     CDS             complement(11139..11729)
                     /locus_tag="P5541_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV71772.1"
                     /translation="MVRKEGAIDLGVEKGSREYFDLVASKWDEMRRSFFSEEVREGAY
                     RAAGLVPGRVAADIGCGTGFITEGLLEKGMKVIAVDQSPAMLAQLLSVMSSPYLDIRQ
                     GEAESLPIDDDSVDYGFANIFLHHVESPPVAIREMTRIIRPGGVLVITDMDEHDFDFL
                     LTEQHDRWAGFDRSDIREWFLGAGLRDVKVGPVGST"
     gene            complement(11943..12392)
                     /locus_tag="P5541_07025"
     CDS             complement(11943..12392)
                     /locus_tag="P5541_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HRV71773.1"
                     /translation="MSQNNDRITGRDDPPDDGEIAGWIGNEDFGYWKRTMEWIEGSYP
                     DVFVPEWIYGGRKHGWSLRFKRSKSFCTLIPEKGRFALLIVFGAEERKKVEAIREGLS
                     LHAREEYDKAETYHDGKWLLLAIDSEKALQDAFSLLTVKRKPKRMNI"
     gene            complement(12524..>13267)
                     /locus_tag="P5541_07030"
     CDS             complement(12524..>13267)
                     /locus_tag="P5541_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71774.1"
                     /translation="GARIRLAPQKDWEVNQPALLEMVLQTLERIQEAFNSAQSGRKKV
                     SIADLIVLGGCAGVEKAAKDAGHDVTVPFTPGRTDATQEQTDAESFAVLEPVADGFRN
                     YTKTRYAVPAEKLLVDRAQLLTLTAPEMTVLVGGMRVLDTNFGQSGYGVFTKRPGTLT
                     NDFFVNLLDMGTMWKAVSEDEDLFEGRARATGELKWTGTRVDLIFGSNSQLRALAEVY
                     GCEDSREKFLHDFVKAWDKVMNLDRFDLA"
BASE COUNT         2934 a         3907 c         3337 g         3089 t
ORIGIN      
        1 tattcagcca cttgcgcctt gatataaact atttgggcaa cagcccgtgt aggttctgac
       61 acctacgtgg gttcagtgga acgcccctct cccgcctcct cctccgtctt tccgtcccgg
      121 atacggtaga tcctgcggaa ggtgggaatt atcttctcgt cgtgggtgac cacaataatg
      181 gccgcctcgt actgcttcgc catggaattc agtatgcgaa tcactgccat tgcccgttcg
      241 ctgtccagag gagcagttgg ttcatcagcc aggatgacag gcggctggtt cgccagggcc
      301 cgggcaatgg atacacgctg ctgctctccc cctgacagct ccgagggcat ggccctggcc
      361 cgatgggcta catcgagggc atccaggagt tctcttgccc ggtcccgtgc ttcgccgttc
      421 ggttttcccg ccagcatggg gagcagggcc acgttgtcgg ttgcgtccag aaaaggaata
      481 agataggggg cctgaaagac gaacccgatc ttgtccctgc gcagcgccct caaatccttt
      541 attttccact tttcctcgta gatcacattg tccccgatgg tcatctttcc cgccgtgggc
      601 tcgataaccg ccccaaggca cttgaggagg gtggttttac ccgaccccga gggaccgatg
      661 agacccacca cctctccggg tgcgaccacc atattgacct ctttcagggc atccacggcc
      721 gtctctcccg acccatagcg cttcttaagc ccttcaaccg taattccttt ccctccggac
      781 atatccttta tccccctatg gcctcggcgg gatccacctt aagggccatc cgaatggcga
      841 gaacgcttga ggcaacgcac atgatcataa cggcgatgaa ccccgccacc gagtcccgtc
      901 cttcaagcaa cacatacttg ggaaagatcg gcgcccagat ggtggcggag atcttgccga
      961 tcatgaaacc aagcaccccg aggccgagag cctgctgaag gatcatcccc acgatggtgc
     1021 ggttttttgt tccgatgagc ttgagaacgg cgatctcacg gatcttcccc atggtgaggg
     1081 tgtagatgat gaaggccacg atggcggcgc tcacgatggc gaggatgacg agaaacatgc
     1141 cgatctgcct ggaagaggtg gcaataagtt tgccgagcag gatctcctcc atctgctctc
     1201 gagtgtacac ttcgtggcgc ttccatcgac ggatatgctc cgcggtctcc tccggatccg
     1261 caccgggagc gaggcgaacg agcacggcgt tcacagtgga atttccggtc tgggaggcca
     1321 gcacgctctc gagcaaccct ggaattcccg gccggttcaa tgaggggttg gcttctgtcc
     1381 gccgacgctg ctgaaggatg aaatcgttat ccttcaggaa ctgtgcctcc tgggcatcct
     1441 tcagggggat gaagaccatt gggtctcctc ccgaggagac cattcgcctg gtgatcccca
     1501 caacccggta ggaatttctg cgtattccga tggagtcgcc gaggcggaat ccggatgcct
     1561 cgtccgccac cgcctcgtaa tgactccggg tgatatgcct tcccgccaca agataccggg
     1621 gctgccccgg cccgccgagg accccgggtt cgatccccac caccatggcc cggacgtcct
     1681 cctccccccg gcgtatctgg gtggtgaggt aggtgacgtt ggccgcagcg tccacccctt
     1741 tcatcccggc cacgctccgc cagagatcgt catagatgct ggacgattcg gcgtagggtc
     1801 cgagagtatc ctgctgaacc acccaaagat ccgctccgct gttgtcgagc agaaccctgg
     1861 cgtcgtccac catcccccgg tagactcctc ccatggtgag ggtcactccg atcaaaagac
     1921 ccagtccgaa gccggtgagc acgaactttc cccatccgtg gagaatgtca cgcccggcga
     1981 ggctgatcat tcttttgcct ccaggttctc cactaccttc acgcggctgc gaggagaaag
     2041 ggcttttttc atgtattgaa ctatattctc ccctttttca aggccgctca tgatctggac
     2101 gaaaccatcg aggtctcttc gtcccgtctt cacaggccgg aaggtaatgg acccgttgtc
     2161 gagaacccac acgccgagat ctccgttcag gcgctggaga gcgctgtcgg gaacgacggc
     2221 ggtctcggga agaggaggaa gagtcaccgt gacctcagca agctcgccca gggggggcag
     2281 cacatctggg accgtgtcga agaccgcctt ggccatcagc tcctccgtga cagcatccgc
     2341 caaaggttcc acccgcagta tcctccccgg aaacacggtg tctccccggg agcggatgga
     2401 gatggaggcg ggaagaccgg gagtgaggcc cgaggccatg gcctggtcaa accggacgga
     2461 tatccacagg ttttcccggt cgatgacctc cacaaccgcc tggcctgcca ccactgtcgt
     2521 ccccggctcg acgttccggg cggtaacgag tccttccgcg ggagagacca gcaagagatt
     2581 gtccttctgc tgctccagtc ccttacggtc ttcccgggcc cgggccaggt ctgcccggat
     2641 ggcgtcccgc tcggcccggg cggaattttc cagcgcggca agtgcctggg cctcctgccg
     2701 ttttgcctgg gcggcgtcga ggctgaccgc gcccaccttc aacaggttgt cataccgctt
     2761 cgccaattcc gaggcgtagt ttcccctggc ggcaacctcg gcgatacggg cctcccctgc
     2821 ctgaagggag gcttccagcc tccggatgga taggtcctga gcggcgagac gttgttccca
     2881 gtccaccgga tccatggcac caaggacctg tcccttctcc acgaggtcac ccacccgcac
     2941 agacagatct tttacccgtc cgggaatggt ggggccgatc cggtaggacg cctgggaatt
     3001 cacggtacca atgccgtaga gggccggggt cagtgagcga acttccacca ccgtagccgt
     3061 cacctccaca ggggccaggg gaccagaacg catcatgaaa tagaagaaga acaccgccaa
     3121 cacaagaaaa cctaagagaa gaaagatcgt cttgcctttt cctttcaggc ccttcatgcc
     3181 ggatccctcc tctcgatgcc ccgccggtag atcgcgaaga ccccgtgggc tttcagaaga
     3241 ccgtcccgtt tgtcctcctc gatctgtgac tggaggacaa gaccctggat ggcccctgta
     3301 aacagggcag ccccaaaggg ggagtccagg gcagggtcga catccccggt cattttcccc
     3361 tcctccagaa gggcaaccac ccgggaatca tattttttga gaagggaatg gacaaggctt
     3421 cgtacggggc tttcctctgt gcgttgaaac tcgctcaaaa ggaggcgtgg cgctcccgga
     3481 tgctccgagg caaactggac atgggcaagg aagatcgcct caagggcttc gaggggggag
     3541 cctgcatcag ccgcggcctt atcaaggcgg gagaagaggc tctttcccac ccagtcgaga
     3601 acggccttcc agatggcttc tttcgtgggg aaatgccgga agagggctcc ctgggtcaga
     3661 ttcatccttt cggcgatggc ggcggtggtg atgtccgagg gtttaccctc ggcggcaagg
     3721 gagatcaccg tctctacgat gacccttttc cgctcctggg cgggaagatt tttgcccttt
     3781 tgaaccatga aaacacccct tctcctaaat agtaagtaat cgcttactat cttaaggaga
     3841 agtgtggaat ctgtcaatgg agatatattc attccaaagg gcagcgacac ctttttggtg
     3901 tcaggccgta cacatttgac acaaccgggc tttggaaggt tttcgctaag ttctttcctt
     3961 actggtatca agaatcatac ttttgcgtta tcagaaccgt gttctatctc cttcactgca
     4021 ccgcagtgct ggcagaaagt aagcagagaa tacctgatat tatggcactt cgggcactcc
     4081 tcgaacagaa ccgttccgca ggccgggcat gagtatggac tgtcttcatc cgatcccctg
     4141 ggaagctcca acttgccgac cgtcctgcgc gtccagaaca agaatttccg tgggcccctc
     4201 ctgatgggaa aatcgcatac cgggcagaag aacctttcat agccctcgcg atactgcttc
     4261 atcagcaggt cggctttagg aaaggcaatg accctgatga aatacaccag gagcttgata
     4321 acggctatga ggagcccacc tacaagaaca tacttgacgt atcgggcagg gaaataccta
     4381 tgtatgacga aagctgtctt cagaagcgta gccgccgcga atccaaggaa aagcggaaag
     4441 taaacgctgc cacgtttcct gaccacaaaa atggcaccag cggccagcat ggggagaagg
     4501 atcagtaact ggtaccccgc cagacgaaga tcgtgtttct cccgaaggac ccggaattcc
     4561 tcccctgcag gcaatgactg cccggctatc ttcttttcaa tggccgatct ttcggactcg
     4621 agagaccttt tacggtcggt gagccgtgat agttccgtgt tgtaaacctg gtaacgcttc
     4681 tggctttcaa ggaaatgggc aagactctcg gaaaggttgg tctgctcctt gtccgaaaga
     4741 gagacggacc tctggactgc cagtttctgg agctctataa gctggttgat ggtattttgg
     4801 aggttctggg agctgtcgtt gacgatacgc atttcctcgc gcttgtcgga aattgcacgg
     4861 tctacgtctg taatttccgc catgagggtt ttcgccctgt cgaccagatc ctggtctaca
     4921 tatctttgtt ctatctcagt atattgagga cctcgaatgg ccccgatatc ctcaacgagg
     4981 aatccgagaa cccagaatac aagaacagcc aggaccacgg taaaaaagta gatcatgaag
     5041 cggtggatcc atggaccttt cgccttattc acagtctcat ctcctcgcgc agccattggg
     5101 gtcacaccta cgctattgac tactcaagat cttccaccat ccgtagcagt tctccttccg
     5161 gatgcctgtc cttttcaaac ctgagttcaa gcctgaggca ggaacgattt acgcacgatt
     5221 cagatatccc gtcaattttt ccggtctcat tcagccttgc cccgctgaaa caatggcaaa
     5281 gatatacccc tgtcgctctg gcaaacctta cgttcccacc aattttgtca aatgtgtacg
     5341 ggctgacacc tctaggactc gggattctga taggtggttg gcccgttggg ccaggacgtc
     5401 gtttccaacc actccccggg atatcccagg ggctgcccca tattaccggg ctcgttcaga
     5461 aggccgtcgt tgtacttaac aatgagctgt tcgctcagat cccaccattc cttcattaca
     5521 gaagcggtat agttttcggc caattccgtc aggtatttca ctgcttctga aggatctttc
     5581 tcgtacagtt gcaacgctct cttctcaact tcgggaagaa agacgagaaa acctttttcg
     5641 agctgctcct ggaggacaac gatctctttg tgcatatagc tgtacttgag tccctgatag
     5701 ttcgaaagga aattgaaggt ccaccatgcg ctctcacggg agaatttctc agtattgcag
     5761 acttcaacag atcggggaag cgacgtcaca ccgacatgaa aggggataaa gacagtatca
     5821 gtgggtttgt ccagccccag ccacatgatc cccccgatcg ggtccggaag ccatccgcga
     5881 gcctggttca cgtaaacgta accgcaataa gatacggaga gcggacgctc ccaggcgcct
     5941 tccagttttc gtgacggatc gcccacatct cctttcccat cgtagggacc gtaaaaacgg
     6001 tcgggatatc cgaacggacc tgccgcaaca ccctttgtga ggtcgaactc tgtgccttcg
     6061 aagtgatcgc gatgaaggcc catgacatcc ctgacggaga gccttttgtc aggtttgaca
     6121 gaaaacgggt aatcttttgt aaatccatcc tccacccacg gggtaaagtt cctggaaggt
     6181 gcaaccaggg ataagacccg ccacacccgt cgaagcgagt agtatgggtg gttgtattcc
     6241 ccgagactca ccgttttcag ccagtcgagt tttccgtcct ctggcttcca ccatccatgc
     6301 ttttgggcaa tttcgtgaag atccttcgag aacatcatgt caggatcatc ggggtccacc
     6361 tcccggatgc gaaactcatt ggcccccacg aagacctccc catcgggaac cttcttcgca
     6421 acccacagac cacctactcc ctcgggagag ggtgccatct ccattaccca cccttcctca
     6481 gtatccccaa caggaagtgt ttctcccgta ccgtagtaac cgtacttctc tatgagttcc
     6541 ccgatcaccc tgatagcctc acgagccgtg cggcagcgct caagagctac cctggacagt
     6601 tcggcagaat agaaaatccg ctttcccggc tccggatcaa gggtgacctt cgatccgtcg
     6661 gtacattccc cgaacattag ctggtgttca ttcataatcc cgtagctgcc gtcaaaatac
     6721 gcataggtct tttccacctg gggaatgtgc ccaagaggaa tggaagcggg acctgcggta
     6781 tcgtaacctc tacccctatc accggtaacc atgcgcggat aggagaaact gttgtactgc
     6841 gggaatgcgc ctatggcagc agcagtgcag aagaccgggc ggagtgcgcc ctgggcgtgg
     6901 tcctgggctg gaacacggat aatgcgctgg tccataagat cgttatcgtc agagtgcgat
     6961 atcatcaccg agccgtcgga ggaagcgccc tttgtgacga ggatagtggt gcaactctcg
     7021 cccggatgta caagtaccag agagagcagc agaagcaacg cgaaaacaga tggtctggat
     7081 ctcattgaca cttcctcctt gaaatgaaat tgtgcaccct aagccggagg gaaaggccag
     7141 cgtcccgagc gacgggacca acccgccccg gttacctgat ttttcttatg acaggctggg
     7201 tcgcacacct gagtgagccg ccctgaccgt tgtgggtccc gaaaaacacc ctgtggactt
     7261 ttatattcct cttctcgagt tcagcctgca gatacctgtt gtcgttgtgt tcattgaagg
     7321 acatgatgta gttctcaccg tcgaccggga tcccgttcac tgtaagcctc cgcacgctct
     7381 cgatatcgac cttgatcagg tcccacccct tgatgtggtc gggcagccca tcaacgaaag
     7441 cttcttcaca aactatcgcc aggcctctcc gaggaacgga gagaacgcag tcgagatgga
     7501 gaacgtcctc cctcagggga acccgtacaa catcatagga gcttcccagt atgttcttca
     7561 gccacctgaa gcctgcttcg ttagatccca cgcttggatt cagagtgttg ccgaccagga
     7621 tagtcctgcc cagtacaatt acgtctccgc cttccaggag cggggtatca ggagagggcg
     7681 gggcaagcaa ttccgtgtgc ggcattgaga accaccgcac tccctccttc gtgcatcttt
     7741 ccagaaggat ttccctgaac cctgaaatat cgacctttct gagaggtgtt cgaagattga
     7801 actctataag gctgttacct atcacggcca tgttgtcacg tgggaaatcc tggctgaacc
     7861 cgttctccag aacatcggac ccataatttt cctttatgaa atcaacggtt atctctctcg
     7921 gccgataaac tttaactcca agggaattta aaatgcctat cagggcaagg ttttcctcct
     7981 ccagcttctc tgtttcgctt ttgccggttt tggggtcgat catatcggtc cagagcattc
     8041 ccgctgtttt tcttgcatac tccgcctcgt ccggcggcaa taccttcatg gcgttttcaa
     8101 accagggcgc gctcaatgag ggcgtcatcc cgaatggaag gccgacgata acttccttca
     8161 ggtctccgta ttcatagtct acgtagaccg gcggttcgta tgcttccgct gttgacaggt
     8221 tgaatgaaaa acacaagctc aaaatgaccg gaaacattat cagtgatcca aggctcattc
     8281 gtgatccctc catgagatgt ggatgcttgc ttgaaggtgc catgcctgca caattgaata
     8341 ttctagtcta atggcattga aaggatcttc tttctttctc cagggtgcta ttctattttt
     8401 ccagattctt cgaaccgctt ccaggtctgg gtcattcccg attttgatat ccgcaaatgt
     8461 ctccgatctc tttcagatag ggtccgttgc caaaaaacat caaaagtcta cggcctggcc
     8521 acgctggatt cgtacttcaa ctgaaaagac cctttaacca gactgcgata aaggcagtac
     8581 tgcctcccag gatcgttccc caggtcatgt ccacaaccgt cacaattacc ggccatctcc
     8641 tgatcgtcgc aaggttcgta agattatatg tcgcgtaaca gatcaaaccg aaaaccgctc
     8701 ccctgaatgc tgcttcttgc agagtccctt cccttccggt cacaaagaag accattcccg
     8761 caatgaaaag gagataaaaa actgccgcag caatccagtt tggtctttct gccattacgt
     8821 agccgatctg tgatctgtag aatttctttg ccacgaggcc cagccagaca gcatccagcg
     8881 caacgaacgg caacaatgta aagagataat tcttcaggaa aagtccggtc attcttttca
     8941 cctccgttgg ttgatccacc attttcaaac attctaggac aaaacggggt tgaccggaat
     9001 ttactgaaag gagcagcggg gtcaggcgtg attaactccg ggttgctgaa gcaaaagtgt
     9061 atctcttgtg aggatcaaag gcgatatact tttattgctg catctcctcc cctgaggcgt
     9121 caaaccgatg ggcccagaaa aaagttgatt tacagcaact ccattggcgt cagactcgcg
     9181 cgccctgtcg ctcaatttag cccccagatt ttccagggcg aaccctttcc gcaatgttta
     9241 tgttggtgta aaagacattg ctgttttccg gctcctggat ccccatcttg gcgaacaacc
     9301 cctggcaata tctgttttaa gggcattcag ctccttgtgc cctgatattc gcaattcggg
     9361 caacaggtcg tacacttttg actatttcag gagcttcgaa acttctgagg gatcaaacct
     9421 gcacttttga ctatttcatt acttttgtct ttccggtgtc ctctggaggg agccaaattt
     9481 gtcccctcag gatacccctc aaggatcagt agcccgtcct ctctagttcc ttcatggcaa
     9541 aggttatctc ccttccatgc tgctgcagat gccatcgttt tcgggcaaca cgggagagaa
     9601 agcgcaggtc atggctgatc agcaaaagag cacagggaca aaccctcaat gcatcctcaa
     9661 ggcactcgat tgccggaata tcgagatgat tggtcggctc atccatgact ataagatagg
     9721 gatggcggat cactccgaga gccagcaaca ctttgcgaag ttccccgggg ctcgcatcca
     9781 ggttgttcaa cagcctttcc ggccttgatc ccagggcact caccacggtc atcaccgttc
     9841 cgagctgctc ccgggaaaga cgatgaacgt cttccattat ctttcgagtc tgggcgaggt
     9901 cgatctcctg ggggagaaat accaggcgtt caccgggaag atccatactg tcgagaatga
     9961 aacgaacgaa agtactcttc cccaacccgt tttcgccggt aatggcaatg cggtcgtccc
    10021 taatgatcga aaactctgga actttcagcc ttctttggcc gtcaaggtcg atgatctccc
    10081 ccgcaatgga aaagaggcat ttgcggggag aaacagaacc ttccagccag aaataggtgt
    10141 catagcgttt cttgatcttt atcccggaaa tcttttcatg cagtctggca atacggcttt
    10201 caggctgtct tgccagccgc ccggcctgcc cgtcctgccc tgaaaccctg gcaagatcga
    10261 ttctggcacg accatcgcta tcccctcgcg ccaggttcct ctttgaactc cttcttcccg
    10321 cccgggaggc tttttcataa cgtcgtctgg attcgatctt cagccgctca aggttctgct
    10381 gcagaatttc acgccttgcg atcaggctgg attcttccct ttgggcatca gagactgctt
    10441 tcgaataatt gcccggcctc accactacct ccgggggatc cagaaaaata cacctttggc
    10501 atagaagatc cagcaaatcc cggtcatggc ttaccagcag tccaatgccg ttgaatgagg
    10561 aaagcgtcgc gacaagaagc tcccgggcga tcagatcaat atgattggtg ggctcatcca
    10621 gaagaaggac atcgggtctc tgccacaggg cgactccgat ctgagcccgt tttcgttcac
    10681 cgtggctgag ggtcggccag cgggtcgccc agttctcctc aacctgcagt tctccacgca
    10741 gcacccatgc ctctgcatca gttgcagata cgaaacgcga cagattctcg ggcatgtcat
    10801 cggtacgctg cgggcaaaac accatgctgc cctttcgttt tatcgtccct gtctgtgcct
    10861 cgatctgtcc catggcaagc tgcagaaaag ttgtcttccc agccccgttc gcgccgacga
    10921 tccccgtcca accttctgca aaatgtaccg ttacatcccc gaatatcggg gacgcgttcc
    10981 cgggataggc gaatgaaacg tgttgaaacg atatcgacat agtagacctc tccaggcctt
    11041 gttctcggga ttgactcaaa aacaggctat ccttttttcc cgctggcgac aaatacactg
    11101 acagcaccga cggtgctgcc ctcatccgat aatgcggatc aggtggaacc aacagggccc
    11161 accttcacat cccggagccc tgcgccaagg aaccattccc ttatgtcgct cctgtcaaaa
    11221 ccggcccagc ggtcatgctg ctcagtcaaa agaaaatcaa aatcatgttc gtccatatct
    11281 gttattacaa gcactccacc tggtctgatg atcctggtca tttcccttat cgcaacagga
    11341 ggtgattcca catggtgcag aaatatgttg gcaaagccgt aatcgacgga atcgtcatct
    11401 atgggaagcg attcggcttc gccctgcctg atatccaggt aagggctgct catgaccgaa
    11461 agaagttgag caagcatggc aggtgactgg tcaacagcta tcaccttcat ccccttctcc
    11521 agcaggccct ccgtaatgaa tccagtcccg cacccaatat cggcggctac cctgcccggg
    11581 acaagtcccg ctgccctgta ggccccctcc cgcacctctt cgctaaaaaa acttcgccgc
    11641 atctcatccc acttcgaggc gaccaggtcg aaatattccc tgctcccttt ttctaccccc
    11701 aaatcgatcg ccccttcttt ccttaccaaa ggtcagaagc ctgccatcct ctcttgtaat
    11761 catactgcat tacggatatg ccttcaacaa ggttcgtcaa taccaccggt gagatcaact
    11821 gaaaaaccat ttaggggaaa agttgcaggc atgcccccgt ccggggtccg caaaacatgc
    11881 tgatcccgtt gctggtgccc ccaggtttaa tatgggccct atggaagaaa ttcagtaaca
    11941 ggtcagatgt tcatcctttt tggtttccgc ttgacagtta aaagcgagaa ggcgtcctgc
    12001 agtgccttct cgctgtctat cgcaaggagc agccatttcc cgtcgtgata tgtttcagcc
    12061 ttgtcatatt cctccctggc gtgaagtgac aggccttctc ttattgcttc gacctttttc
    12121 cgttcttccg cgccaaagac gataagcaat gcgaaacgcc ccttctcggg gatcaacgta
    12181 cagaaagatt tcgatctctt gaacctgagg gaccagccgt gtttcctccc gccgtagatc
    12241 cattccggga caaagacatc ggggtaggat ccttcgatcc attccatcgt ccgcttccag
    12301 tatccaaagt cctcattgcc tatccagcct gcaatttctc catcatccgg aggatcatcc
    12361 ctgcccgtta tacgatcatt attttggctc atcgtccttc aggattgttg cctgtcagat
    12421 ccggaaaggc ccaaaaacct gaacaggagg cctccgcaag gatcgatcct ttaaccagtt
    12481 tctccaatgt gtacggctcg aaacctgtaa ggttactctg ggatcaggcc aggtcgaaac
    12541 ggtcaaggtt catcacctta tcccacgcct ttacaaaatc gtgcaggaac ttctcccggg
    12601 agtcctcgca tccgtagacc tcagccaaag ccctgagctg ggagttcgaa ccgaatatga
    12661 ggtcgacacg ggtgccggtc cacttgagtt ctcctgttgc acgagcccgg ccctcgaaca
    12721 ggtcttcgtc ttccgatacc gccttccaca tagtgcccat gtcaagcagg ttcacaaaga
    12781 aatcattcgt gagagtccct ggccgctttg tgaagacacc gtacccggac tgtccaaagt
    12841 ttgtatccag aacgcgcatc ccgccaacga ggaccgtcat ctcaggagca gtcaacgtca
    12901 gaagctgcgc tcgatcgacc agcagttttt ccgccggtac ggcgtacctg gttttggtgt
    12961 agttgcggaa cccgtccgca actggttcga gaacggcaaa tgactctgcg tcggtttgct
    13021 cctgcgtcgc atccgtgcgt cccggcgtga agggaacggt cacatcgtga ccggcatcct
    13081 ttgcggcttt ttcaacgcct gcgcatccac ccagcacgat aaggtcggct attgaaacct
    13141 tcttccggcc ggactgcgcg ctgttgaacg cctcctggat ccgctcaagt gtttgcagca
    13201 ccatttccag cagggccggc tggtttactt cccagtcctt ttgcggtgcc agacggatac
    13261 gggcccc
//
LOCUS       DAOTIQ010000051        13135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5661_length_13135_cov_6.653364, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000051 DAOTIQ010000000
VERSION     DAOTIQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_13135_cov_6.653364"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>1123
                     /locus_tag="P5541_07035"
     CDS             80..>1123
                     /locus_tag="P5541_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71775.1"
                     /translation="MKENRSSIALYAAAFIQMVGVGLIVALLPSKVIALSGSMANVGL
                     IASAFAVPFVLLQLPVGNLGDRFGHKRFLVAGYVIAGLTGFLYFKAESVNSLLFGRVL
                     QGFAEIPVWALAPALLSIFFAESKGEAIGRYNASIHLGLTAGSLLSVYVHSVWSGNEA
                     FLLYAASGFTGALIVAFMARGAEQGRQAIPGSGADLRAVFRALYMISRPAIHGGVIIY
                     GAFYGIFLTVIPAVLLSVKGFSQSEVGAFFALFYIAISVSQVAAGRVADKIGPNPTIF
                     AGLLLVASGVSAFMLFSGAAVLATLFVASFGLGMFCVSAMVLLNEAVPVSLKGSVSGV
                     FYLLWGIGFFLAPP"
     assembly_gap    1124..1223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1224..2200)
                     /locus_tag="P5541_07040"
     CDS             complement(<1224..2200)
                     /locus_tag="P5541_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HRV71776.1"
                     /translation="MILIDPQRGVTQLSLLGAVAALSVSRGTESCTLPDRLALPGNTV
                     ITIRLSMEEKAGSLHASISFSVEGKHSDLSGFSCDDACRLLEEQGSHKPLEKDDAIDF
                     IRALHRMEAESGKPGDVLSWPERALSAFSFFRIFNELDMPMESLPAGIGTERNEGVVS
                     IASEYRIPLKGIPSNGTRTTTSGLLILARSVIFREALPSFIPIRQAQGTDPEGGCVRT
                     ILTHESPEEEIWIAEASMDDATGEEMGRALEEMQSVSLEAHILQGMGKKGRPLFILRA
                     LASLEQLDEVLDRFFKDTPTIGVRYWPVGRTRMHRETKEGQLVVDGISLP"
     gene            complement(2202..3011)
                     /gene="larE"
                     /locus_tag="P5541_07045"
     CDS             complement(2202..3011)
                     /gene="larE"
                     /locus_tag="P5541_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRV71777.1"
                     /translation="MNRLDKADKVRAFFRDNPSTLVLLSGGVDSAVLALLASEGAPGE
                     TKALTFRTPLVKEDEIEMAEAVAEMLDIDLQIEDIDVTSDPQVSANPGDRCYFCRKIL
                     HGEATKYARTAGLTAIADGVQADDLVEGRPGVRSAAEDGILHPLAEAGLTKSEIRELA
                     RSAGLPNSERPAAPCIATRFPPGVPISRVWLERLEKGEAFLAGESFENSRIRLFPPGL
                     ASIEIEEHDMQRFWEIRKRVFETLHEIGFPVVSLDIEGLKRGKMERFLEVE"
     gene            3321..4085
                     /gene="larB"
                     /locus_tag="P5541_07050"
     CDS             3321..4085
                     /gene="larB"
                     /locus_tag="P5541_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRV71778.1"
                     /translation="MDEREIRLLADKLRNGEISVDTFVRSFKGLPFRDLGEVKLDTHR
                     ALRGAFPEIVYCPGKSPDQLKRIAETFSELPGRALFSRMSQEQHELVSTILPGLEYLP
                     VPRIGRLWPDRDLPSKGLVLVTTGGSSDVPVAEEAAVTAETIGCEVRRLFDVGIAGLH
                     RLIAHMDVLFDADAIVAVAGMDGALPGVVAGIASCPVVAVPTSVGYGVAMNGIGPLIT
                     MLNSCALGVSVVNIDNGLGAGYTAALIANRSSKNIH"
     gene            4098..4814
                     /locus_tag="P5541_07055"
     CDS             4098..4814
                     /locus_tag="P5541_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HRV71779.1"
                     /translation="MKKILYLNCFSGISGDMMLGLLLDLLGKVDINPFLEGLALEGYV
                     VEASRSKRGGISGVDVRVRCDEKHCHRGLKEVLEVIEKSDITPEASHKSETAFRLLAE
                     AEGAVHGMPPESVHFHEVGAVDSIVDIVGSCILMDVLAPGLVISSPVNVGSGTVKCAH
                     GELPVPAPATMKLLEGIPVFSRGQPMERTTPTGAVLLKTFVDSFGTIPPGVVERTGYG
                     LGDRESDLPNVLHGLMIMAN"
     gene            4871..6346
                     /gene="mptA"
                     /locus_tag="P5541_07060"
     CDS             4871..6346
                     /gene="mptA"
                     /locus_tag="P5541_07060"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HRV71780.1"
                     /translation="MKNSPVLQRAYLLLGANLGDRQGNILRALQLIGGRNMIEEISSF
                     YETMPAGHQDQPWFINLACRIETDMDPSDLHRFVKSVELLMGRDDHNKGFPRPIDIDV
                     LFYETASPLGDGFRKTYPGLGERAFAIAPLAEIAFGIIPPGFTKNVGEIAKEMDMSGI
                     RRVENSLRLKLERDIQQENPRVPASLSRVGVTGLKRILRIENGGKVSLFYAEMDLFAW
                     LGPNQAGVHMSRFSDVLEDLVEEITLEASPNIETLAGRLARKISLTQQGGRSEVVIRA
                     KFPIRKITPISGKNVEELYTFIGISACAGESQRTAVGVEVEGMTVCPCAQDMVRSHSR
                     KLLLGDGFSEEETDRILGLVPLASHNQRGRGTLLVGGNRPLKAEHLVHILEASMSSET
                     YELLKRPDEFFVVNKAHKNPMFAEDVVREVLRNLLDMYPDLHDDTFVLVRQENLESIH
                     QHNAFAERSGTLKAIRSEIKQGFSSVEDLSLQEWLKGGKDR"
     gene            complement(6347..6730)
                     /gene="queD"
                     /locus_tag="P5541_07065"
     CDS             complement(6347..6730)
                     /gene="queD"
                     /locus_tag="P5541_07065"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRV71781.1"
                     /translation="MFLKKDFSFDAAHKLEKYHGKCEALHGHTYRFQVTLEGRPDSEG
                     MIMDFIELKKIVGDRVLSRLDHSYLNEIIPQPTAENIGLWIWSELESEILRENCCLWS
                     VQVWETPSSSIVIERKDIQVKGRHS"
     gene            6917..8053
                     /locus_tag="P5541_07070"
     CDS             6917..8053
                     /locus_tag="P5541_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRV71782.1"
                     /translation="MKGPSRHFTLFSVVVFIAVLCRAFAGIASGLTVTDAWGREVAIP
                     ENVSKVICSGPGCMRYLVYLQAEDLAVAVDDMEKARNMFESRPYFIAHPELKDKPLFG
                     EFRGHDNPELIVALDPAPQVIFKTFGNMGHDPVELQKKTGIPVIVLEHGQLGVGKKQM
                     DDTLLMMARVIGKEERAKEVIAFFDSAIEDLRERTADIPEEKRPTCYIGGVAYKGPHG
                     IISTEPAYPPFIFVNAVNIAADPSKTKSDQLAQTTFSVESLLSMDPDRIFVDLSTLQG
                     GTEVNGLFQIRDGAPFQVLTAVEKGEIYGVLPYNWYSQNHGSILADAYFIGKVLYPDR
                     FEDIDPVKKADEIYAFLVGKPVFEEMDRSFGGYVFKKIDLSKVE"
     gene            8058..9128
                     /locus_tag="P5541_07075"
     CDS             8058..9128
                     /locus_tag="P5541_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV71783.1"
                     /translation="MHLEEGTVPAGYSKYIQAKVFLLLLGLVLLLVLAVFSISTGPVS
                     YDPLEVLRTLFMDHVSRQLDVVVFNIRLPQVLAAILAGAGLSVAGVVMQSVLGNPLGS
                     PFTLGISQAAAFGAAFSVMVLGSGFIQSSASDAITVVRPGMTTASAFAAAMIATGVVV
                     MIARIRRGSPEVMILAGVAIGSLCTAATMFLQYFADDVQLAAMVFWTFGDLARAGWRE
                     IAVISFVGGLCLFYFVVNSWNYNAIDAGDETAASLGVNVKVVRFLGMTLASLVTAVII
                     SFVGVIGFVGLVCPHMARRLTGDDNRFLIPGSCLMGSILLLAADTAARVMMAPNVLPV
                     AILTSFVGAPMFLFLLVRGYRK"
     gene            9125..9883
                     /locus_tag="P5541_07080"
     CDS             9125..9883
                     /locus_tag="P5541_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71784.1"
                     /translation="MILSVSNVSFRYNSRPVLDDVVFNLGRNELLAILGPNGVGKTTL
                     LKCLNGILKPTAGAIMVEDRDLLQMGQMEIARELAYVAQQGQGGRFTAFDAILLGRRP
                     HITWRVGKEDIRKVDAIIHALGIDHLSLRHLDEMSGGELQKVSIARALVQEPRILLLD
                     EPTSSLDLKNQQEILSTIRHIVTGHGMAAVMTMHDINSALRFADRVIFMKGGEIFAVT
                     DLKGITPQMVRDVYDIPVTIESVRGYPVVVPVGN"
     gene            10251..10862
                     /locus_tag="P5541_07085"
     CDS             10251..10862
                     /locus_tag="P5541_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRV71785.1"
                     /translation="MRSKISEEELKAVIDFHGHWCPGLATGIRVAEVALEALGRAGDE
                     DIVAVAESDSCAVDAVQFLTGCTVGKGNLIIHNIGKMAFSFYRRRDGKAFRIVSRSNV
                     ARGTSDEYRKLQDKKNQGTITSEEAARLQELRQEHCARILGSDIDDIFVTGEPLEPVP
                     PFAPMKKSLVCDECGEMIMETRARLSDGRTLCPPCFERLVPRP"
     gene            10920..12068
                     /locus_tag="P5541_07090"
     CDS             10920..12068
                     /locus_tag="P5541_07090"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRV71786.1"
                     /translation="MGRSFALRVVFFALLSILFGWGMTTLSEGVIASKWTFEDSWVVK
                     MFEVSEVPVEETEEGVLFDEWRTEALDIDVLFLSGEYRGTRAVARVEHLKDNKLDLRP
                     GLHYILSYNEFEDGSRIFFVSDIFRAPVFVGLFTGAVIFFCLVTGLTGIRALVGLFLS
                     FVILVKLLLPGLLKGYPPQALGVAAVAGVALVTILSVVRRRRCWPSAFSGALGGCVSA
                     LVFGWGSIFMLRLSGLSTATASLLASTVPGIDLRGILLASIIIGASGAVLDVAVSVTS
                     AMAEMKDHDPGIEARVLFQSGINVGKEVLGSMVNTLVFAYFGNSLVLSLLILEAEPIP
                     IAMLNDSVIAEEVIRALAGTAGLLLTIPLTALFGALMMSGGSVQSGEG"
     gene            complement(12195..>13135)
                     /locus_tag="P5541_07095"
     CDS             complement(12195..>13135)
                     /locus_tag="P5541_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRV71787.1"
                     /translation="GFDSLTGAGIVLAGAGAGFAAAFMNPFTVGVAQGIAELPLFSGM
                     GYRIFCYISMLALTVAFIYWYAGRVKKDPARSLMYEIDKTREEVLDLNVMKEFTASHQ
                     LVLLVLAGTMVLLVFGVMKYGWYIQEISGLFLGMAILVGIVGKLGLNGFAINLADGMA
                     ALTTGALVVGFARAILVVLTQGNILDTILYGAATAVASLPSIFSAMGMYVFQCLLNYI
                     VPSGSGQAAVSMPIMAPLGDLVGVTRQVAVLAYQFGDGISNIFTPTSGYFMAGLALAK
                     IPWDRWAKWFLPLILLQYLLGAILVTIAHFIKLGPF"
BASE COUNT         2891 a         3349 c         3682 g         3113 t
ORIGIN      
        1 ccctcttcgg taaaatcgtg ggatcagtaa tttcgcaagg aaaaattccc tggcaccaaa
       61 aagagaggcg aataaagtat tgaaagaaaa caggtcatcc atagcactat atgcagccgc
      121 gttcatccag atggttggcg tgggattgat cgtggcactt cttcccagca aggtcatcgc
      181 cctttctggc tcaatggcaa atgtgggcct gatagcttcc gcctttgcgg ttccctttgt
      241 ccttctgcag ctccccgtcg ggaacctggg ggacaggttc ggccataaga ggttccttgt
      301 tgccggttac gtcatagccg gcctgacagg cttcctgtac ttcaaggcgg agagcgttaa
      361 cagccttctt tttggaagag tcctccaggg atttgcggag atccctgtct gggccctggc
      421 tcctgcgctt ctgtcgatct ttttcgccga gtccaagggt gaggctatcg ggaggtacaa
      481 tgcctcgatc catctgggac ttacagcagg cagcctccta agcgtatatg tccattccgt
      541 gtggagcgga aacgaagcct tcctcctcta cgctgcctcg ggctttacgg gagctcttat
      601 tgtcgcgttt atggcaagag gggctgaaca gggcagacag gcgattccgg gaagcggtgc
      661 ggatctcagg gcagtttttc gagccctgta catgatctcc cgacctgcca tccacggggg
      721 agtaatcatc tacggagcct tttacgggat attcctgacg gtgataccag cagtactcct
      781 gagcgttaaa gggttttccc agagtgaagt gggagccttt ttcgcccttt tctacatagc
      841 gataagcgtt tcacaggtcg ctgctggaag ggtggcggac aagatcggtc cgaacccgac
      901 aatattcgcc gggcttctcc ttgtagcctc gggcgtttca gccttcatgc tcttcagcgg
      961 ggcggcagtc ctggctacgc tcttcgttgc cagtttcggc ctggggatgt tctgcgtctc
     1021 ggccatggtc ctgcttaacg aggccgttcc cgtatccctc aagggttccg tttcgggggt
     1081 cttctacctt ttatggggta tcggtttctt cctggcgcct ccgnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnggcaggc tgatcccgtc aacgacgagt tgtccctctt
     1261 tcgtctcacg atgcatcctg gtccgtccca caggccagta gcgcaccccg atcgtcgggg
     1321 tatctttaaa gaagcggtcc agaacttcat cgagctgttc caaagaagcc agggcacgca
     1381 ggataaaaag gggtcttccc ttctttccca tgccctggag gatgtgagcc tccagcgaga
     1441 cggactgcat ctcctccagt gcccttccca tctcctctcc ggtggcatca tccatcgatg
     1501 cttcggctat ccatatctcc tcctccgggc tttcatgagt caggatcgtc ctgacgcatc
     1561 caccctcggg gtcggtcccc tgcgcctgcc tgatcgggat aaaagacggc aaagcctccc
     1621 tgaagatgac tgacctggcc aggatcagga gcccggaggt ggttgtgcgg gtcccgtttg
     1681 aagggattcc tttcaggggt atccggtatt ctgaggcaat ggataccacg ccttcattcc
     1741 gttctgttcc gatacccgcc ggaagcgatt ccatcggcat gtcaagttcg ttgaagatcc
     1801 tgaaaaacga gaacgcggac agagcccttt cgggccatga cagaacgtct ccaggcttcc
     1861 ccgactcggc ctccatccgg tgcagtgccc ggataaaatc tatcgcatca tctttttcca
     1921 gaggtttatg ggatccctgt tcttcaagaa ggcggcatgc atcatcacag gaaaagcctg
     1981 acaggtcaga atgtttgcct tcaactgaaa aagagatcga ggcatgaagg gagccggcct
     2041 tctcttccat ggaaagccgt attgtgataa ccgtattccc gggcagtgca agacggtctg
     2101 gaagggtgca actttctgtg cctcgcgaaa cagacagggc ggcgacggcc cccaatagtg
     2161 aaagttgtgt caccccgcgt tgagggtcga taaggatcat ttcattccac ctccagaaag
     2221 cgctccatct tgccgcgctt caggccttcg atatcgaggg aaactaccgg gaaaccgatc
     2281 tcatggaggg tttcaaagac ccttttcctg atctcccaga accgctgcat gtcatgctct
     2341 tcaatctcga tcgaggccag gccgggaggg aaaaggcgga tccttgaatt ttcaaaactc
     2401 tccccggcaa ggaatgcctc acctttttcc agacgttcta accagacccg ggaaattggg
     2461 accccgggag ggaatcttgt agcaatacat ggcgcggcgg gtctttcgct gtttggaaga
     2521 cctgctgagc gggccagttc ccggatctcg ctttttgtga gacctgcctc agcgaggggg
     2581 tggaggatcc cgtcctccgc ggcagacctt acgcccggcc tgccttcgac caggtcgtca
     2641 gcctggactc catcagcgat ggcagttaaa cctgcggttc tggcgtattt cgtggcctcc
     2701 ccgtggagga tcttcctgca gaagtagcag cggtccccgg ggtttgcaga gacctgggga
     2761 tccgaggtaa catctatgtc ctcaatttgg aggtctatgt caagcatttc ggcaactgcc
     2821 tcagccattt ctatttcatc ctcctttacc aggggggtcc ggaaagtcag ggcttttgtt
     2881 tcgcccgggg ccccttcgga cgccaacagg gcaagaacgg cgctgtcaac tccgcccgag
     2941 agaagcacaa gcgtcgaagg gttatccctg aagaatgccc ggaccttgtc ggctttgtcc
     3001 aatctgttca aggatgttca tccttccttt tctgtctgga ccctcaatca gaaacgatac
     3061 cacaattctt gtcattgatg cggtaggcaa tcccgggatc ctttctcaag gttgtgctga
     3121 tccaaaagat catggaccgg aattgatcca acaggatctc gtttcaggaa ggacaggctg
     3181 tctggagaga gacaactttt cgagtataat tatctgaaat ccctaaatca aggttttgag
     3241 gcgctcaggg gtgtattgca acaacagtga agatgttttt ccccctggtg tctcaacaat
     3301 gaaatggagc atgtaagccg atggacgaaa gagagatccg cctgctggcg gacaaactga
     3361 gaaatggcga gatctcggtg gataccttcg taagatcatt caagggtttg cctttccgcg
     3421 atctcgggga agtaaagctt gacactcacc gggctctgag gggtgccttt cctgaaatag
     3481 tctactgtcc cggcaagtca ccggaccagt tgaagcgaat tgctgagacc ttttccgagc
     3541 tccccggcag ggctctgttt tcccgaatgt cacaagagca gcatgaactt gtttcaacga
     3601 tccttccagg tttggagtat cttccggtac ccaggatagg gcgcctttgg cctgaccggg
     3661 accttccgtc gaagggactt gtccttgtga ccaccggcgg gagcagcgat gtccccgttg
     3721 cggaggaggc ggccgtcacg gccgagacca tcggctgcga ggtcaggagg ctgtttgatg
     3781 tgggtatcgc aggacttcat cgcctgatag cgcacatgga cgtccttttc gatgcagacg
     3841 caattgtggc cgtggcaggc atggacgggg ctcttcccgg ggttgtggcc gggatagcaa
     3901 gttgcccggt ggtggctgtg ccgaccagtg taggttacgg tgttgccatg aacgggatag
     3961 ggcctctcat aacaatgctc aactcatgcg ccctgggagt ttcggttgta aatatcgaca
     4021 atgggctcgg ggccggctac acggctgccc ttatagccaa cagaagttcg aaaaatattc
     4081 actgagggag aatcccgatg aaaaagatcc tttaccttaa ttgcttttct ggcatcagtg
     4141 gtgacatgat gcttgggctt cttctggacc ttcttgggaa ggtcgacatt aaccctttcc
     4201 tggaggggct tgctcttgaa ggctacgttg tggaagcctc cagaagcaaa agaggcggca
     4261 taagcggggt tgatgtcagg gtcagatgcg acgagaagca ttgccacagg ggattgaaag
     4321 aagtgcttga ggtaattgaa aagagcgata ttacccccga agcatcccat aagtctgaaa
     4381 ccgctttccg gctgctcgcg gaggctgaag gcgcagtcca tggaatgccc cctgaaagcg
     4441 ttcatttcca cgaggtcggt gctgtggatt ccatagtcga tatagtcggt tcatgcatcc
     4501 tgatggatgt ccttgccccg gggctggtca tatcctcgcc ggttaacgtg ggttcgggga
     4561 ccgtgaaatg cgcccacgga gaactacccg taccggcacc agcgacaatg aaactgcttg
     4621 aaggcattcc cgtattctct cgcggacagc cgatggaacg tactaccccg accggggcag
     4681 tcctgctgaa gacatttgtc gacagctttg gcaccatccc cccaggggtg gtcgaaagga
     4741 cgggttacgg gctcggtgac agggaatcag atctgccaaa cgtacttcac ggtctgatga
     4801 tcatggcaaa ctgaccaccc ttcccttgca gtttcatgga tgagttgacc ttactgaaag
     4861 ggggggtttg atgaagaata gccccgtatt gcaacgtgcc tatctcctcc ttggggcaaa
     4921 tcttggcgac agacagggta acatcctaag ggcactgcag ttaattggcg gaaggaacat
     4981 gatcgaggag atctcttcct tttacgagac catgcccgca ggccatcagg atcagccgtg
     5041 gttcatcaac ctcgcctgca gaatagaaac ggacatggac ccttccgacc ttcatcgttt
     5101 tgtcaagtct gtagaactcc ttatgggaag ggacgatcac aataaaggtt ttcccaggcc
     5161 cattgacata gatgttttgt tctatgaaac tgcctctccc ctgggggatg ggtttcgcaa
     5221 gacttatccc ggccttggcg aaagggcttt tgccatagcc ccgctggcag agatagcttt
     5281 cgggatcatt ccgccaggct ttaccaaaaa tgtcggagag atagcaaaag aaatggatat
     5341 gtccgggatc aggagggttg agaacagcct tcggctgaaa ttggagagag atatccagca
     5401 ggaaaatccc agggtccctg ccagcctgag ccgggttggg gtcaccggat tgaagaggat
     5461 ccttcgcata gaaaacggag gaaaggtctc gttgttctac gccgaaatgg accttttcgc
     5521 gtggttgggg ccaaaccagg caggggttca catgtcccgc ttctctgatg tgctggaaga
     5581 tctagttgag gagataaccc ttgaagcttc gccaaatatt gagactttag cggggaggtt
     5641 ggccagaaag atatccctta cccaacaggg tgggaggtcc gaagtagtca tcagggcaaa
     5701 gttccctatc cggaagataa ctccgatctc cggcaagaac gtggaagagc tgtatacctt
     5761 tattggcatc tctgcctgtg ctggtgaaag tcaaaggaca gccgtcggcg tcgaggtaga
     5821 agggatgacc gtatgtccct gtgcccagga catggtccgg agccactcca ggaaactgct
     5881 attgggagac gggttcagcg aagaagaaac cgaccggatc ctgggacttg ttcctttggc
     5941 ctcgcacaac cagcgcggac gagggaccct tctcgtcggg ggtaacagac ctctgaaggc
     6001 agaacacctg gtccacatac tggaagcctc gatgagttcc gaaacctacg aactgctcaa
     6061 acggcccgac gaatttttcg ttgttaacaa ggctcacaaa aaccccatgt tcgcagagga
     6121 tgtggtgcgg gaagtgctca gaaatcttct ggatatgtat cccgaccttc atgacgacac
     6181 ttttgttctt gtcaggcagg agaatcttga gagcatccac cagcacaatg ccttcgcaga
     6241 aaggtcgggg accttgaagg ccatccgttc agagatcaaa cagggatttt catccgtaga
     6301 ggatctctct ctgcaggagt ggctcaaggg gggcaaagat cgctgatcaa gagtgtcggc
     6361 cttttacctg aatgtccttt ctttcaataa caattgaaga agagggagtc tcccacacct
     6421 gtactgacca gaggcaacag ttttcgcgaa gtatctcact ctccagttca ctccatatcc
     6481 agaggcctat gttctccgca gtcggttgag ggatgatctc gttaaggtat gagtggtcga
     6541 gccgtgacag gaccctgtct ccgacgatct tcttcagctc tataaaatcc atgatcatcc
     6601 cttcactgtc gggcctgcct tccagggtaa cctggaaacg gtaggtgtgg ccgtgaaggg
     6661 cttcgcattt cccgtgatac ttctccagct tgtgagccgc gtcaaaagag aaatcctttt
     6721 tgaggaacat gatctcccac tccctgtttt ttcggcttta ccccaggaga aacaatatgt
     6781 cctgattata aatgatgatc ctcatttgct tcttaagcat tttcagttgc cctggatggc
     6841 tttaccctct tgctcttgca agtattacga aatagaataa tcgtaataac agaaaaatgg
     6901 ggtgacagca ttgtgtttga aaggaccttc caggcacttt acacttttct ctgtcgttgt
     6961 tttcattgcg gttctctgta gggcatttgc aggcatcgct tcagggctaa cagttaccga
     7021 tgcctgggga agggaagtgg cgatccccga aaacgtgtcc aaagtgatct gttccggtcc
     7081 cgggtgcatg cgatatcttg tctacctcca ggcagaggat ctggccgtgg cagtagacga
     7141 catggagaag gccaggaaca tgttcgaatc ccggccatat tttattgccc accctgaatt
     7201 gaaggacaaa ccacttttcg gtgagttcag ggggcacgac aacccggaac tgatagttgc
     7261 ccttgacccg gccccacagg tcatcttcaa gacctttggt aacatggggc acgacccggt
     7321 ggaattgcag aagaagacgg ggataccggt tattgtcctt gaacacgggc aactgggtgt
     7381 gggcaaaaag cagatggatg atacactttt gatgatggcg cgtgttatcg gcaaggagga
     7441 gagggcaaaa gaggtcatag ccttcttcga cagtgccatt gaggatctca gggaacggac
     7501 tgccgacatc cccgaagaaa aacgaccgac ctgctatata ggtggcgtag catacaaggg
     7561 gccccacggg ataatttcga ccgagcccgc ctatcctccg ttcattttcg tcaatgccgt
     7621 gaacattgcg gcggacccat cgaagacgaa gtctgatcag ctggcgcaga cgacattttc
     7681 cgtcgagagc cttctgtcga tggatccgga caggatcttt gtcgatcttt ctacccttca
     7741 gggaggaact gaggtcaacg gcctctttca gatacgcgac ggggcaccct tccaggtcct
     7801 cacggcggtg gaaaaaggtg agatctacgg ggtcctgccc tataactggt attcccagaa
     7861 ccacgggtct atccttgccg acgcatactt catcggcaag gtcctttacc cggacagatt
     7921 tgaggatatc gacccggtaa agaaggctga cgagatatac gcgttccttg tcgggaagcc
     7981 tgtattcgaa gagatggaca ggtctttcgg cggttacgtc ttcaaaaaga tagatctttc
     8041 aaaggtggaa taggtaaatg catctggaag aagggacagt cccggcggga tactccaaat
     8101 acatccaggc aaaggttttt ttgctcctgc tgggactcgt ccttctcctg gtgcttgcgg
     8161 ttttctcgat atccacagga cctgtgagct atgatcccct ggaagttctg agaactcttt
     8221 ttatggatca cgtctccaga cagcttgacg tagtggtttt caatatccgg ctcccacagg
     8281 tccttgccgc tatcctagcc ggagcgggtc tttcggttgc aggtgtggtg atgcagtcgg
     8341 ttctgggaaa ccctctaggt tccccgttca cgcttgggat atcccaggcg gctgctttcg
     8401 gcgcggcttt ttcagtgatg gtattagggt ccggctttat ccagagttcc gcatcggacg
     8461 cgatcaccgt ggtccggcca ggtatgacga ccgcttcggc cttcgccgcc gcgatgatag
     8521 ctacgggagt ggtggtcatg atcgcccgga tccgaagggg ttccccggag gtcatgatcc
     8581 ttgcaggtgt agcgataggt tccctctgca cggcagcaac aatgtttctg cagtactttg
     8641 ccgatgatgt ccagttggct gccatggttt tctggacctt cggcgacctc gccagggccg
     8701 ggtggaggga aatagcggtc atttctttcg tcgggggtct ttgccttttt tatttcgttg
     8761 tcaattcatg gaattacaac gctatagacg cgggcgatga gacggctgca agccttgggg
     8821 tcaatgtgaa ggtggtccgc ttcctgggaa tgacccttgc ttcgctcgtc actgctgtca
     8881 tcatatcttt tgtgggtgta ataggttttg tgggtctcgt atgtcctcac atggcaaggc
     8941 gattgacagg ggacgacaac aggttcctca tccccggttc ctgcctgatg ggctccatcc
     9001 tccttctcgc tgccgatacc gcagccaggg tgatgatggc tcccaatgtt ctccctgtgg
     9061 ctatactgac ctcttttgtc ggagcgccga tgttcctctt ccttcttgtc agggggtacc
     9121 gcaaatgatc ctcagtgtca gcaacgtctc ctttcgatat aacagcagac ccgtactgga
     9181 cgatgtcgtt ttcaacctgg gcaggaacga actgctggcc attcttggcc ccaacggtgt
     9241 gggcaagacc acattgctca aatgtctgaa cggtatcctg aaaccgacgg ctggagccat
     9301 catggtggag gacagggacc ttcttcaaat ggggcagatg gagatagcca gagagcttgc
     9361 ctatgtggcc cagcagggcc agggtggcag gtttacagcc tttgacgcga tcctcctcgg
     9421 acggagaccc catattacct ggagagtcgg caaggaagat atccggaagg tcgacgctat
     9481 cattcacgcg cttgggatag atcacctctc cctgaggcat cttgacgaaa tgagcggggg
     9541 ggaactgcag aaggtaagca tcgcgagagc cctggtccag gaaccgagga tcctcctgct
     9601 ggacgaacct acgagcagcc ttgacctgaa aaaccagcag gaaatacttt cgacaataag
     9661 gcatattgtc acgggtcacg gaatggctgc ggtcatgaca atgcatgata taaactccgc
     9721 cctgcgtttt gccgacaggg tcattttcat gaagggcggg gaaatatttg cagttactga
     9781 cctcaaaggg ataacgccgc agatggtcag ggatgtttac gatattcctg tcaccataga
     9841 gagcgttcgc ggctatcccg tggtggtgcc ggtggggaat taatagacgt taatcgtgaa
     9901 tcgtaaatcg ggaacctaaa ggcgtaaacg gcacccagtc gctatcgctc cgggcactgg
     9961 gtgaacggta aacagtgaac ggtgaacggt cacggaaaaa ccggaaagca aaggcaaagc
    10021 cttgtcattc tgagcggagc gaagaatctg gttttgtccc ttcgattcac gatttacaaa
    10081 aggacagatc cctccccttg cgggttcggg atgacagctc cttcggttca cgactcacga
    10141 ctcacgattc acgattaacg aaaggaacag acatctcacc cttcggggtt cgggatgaca
    10201 ggcccttcga gaaattggca caatagaatt aatccaatgg aggtaatgtt gtgagatcaa
    10261 agatatctga agaggaactg aaggctgtta ttgattttca cggccactgg tgtccggggc
    10321 ttgccacggg gatacgggtg gctgaggtgg cccttgaagc attgggtcgg gctggggacg
    10381 aagatattgt agccgtcgcc gagtcggata gctgtgccgt cgatgctgtt cagttcctga
    10441 caggttgcac cgtgggtaaa gggaatctca taattcataa tatcggcaag atggccttca
    10501 gtttttatcg cagaagagac ggcaaggctt tcagaatagt gagcaggtca aatgttgcga
    10561 ggggtaccag tgatgagtac agaaaacttc aggacaagaa aaatcaggga acgatcactt
    10621 cggaagaggc tgcaagactt caggaactca ggcaggagca ctgtgcccgt atccttggtt
    10681 ccgacatcga cgatatcttt gtaacagggg agcctcttga gccggtcccg ccctttgccc
    10741 cgatgaagaa aagcctggtc tgcgacgaat gcggcgagat gatcatggag acaagggcca
    10801 ggttgtctga cggacgtaca ttgtgccctc cctgttttga aaggcttgtc ccccgtccgt
    10861 gaggtttatc ctggtgtaga cggttcggga accttatcct tgtctggatg gtgataggcg
    10921 tgggcagatc cttcgcgctc agggtagtgt tttttgcgct tttgtcgatc ctgttcggat
    10981 gggggatgac gaccctgtca gagggggtca ttgcttcaaa atggactttt gaggacagct
    11041 gggtagtgaa aatgtttgaa gtatccgagg tccctgtcga agaaacagaa gagggcgttc
    11101 tttttgacga gtggaggacc gaggccctgg acattgatgt gctctttttg agcggtgagt
    11161 atcggggcac aagggcggta gccagagttg agcatctgaa ggacaataaa ctggatctcc
    11221 gcccgggatt gcactatatc ctctcttaca acgaattcga agacggaagc aggatctttt
    11281 tcgtcagcga tatcttcagg gcgccggttt tcgtcggact gttcacagga gcggtgatct
    11341 tcttctgcct tgtgacaggg ctcactggga tacgggccct tgtcggtctt ttcctttctt
    11401 ttgtaatttt ggtcaaactt cttcttccag gacttctcaa gggatacccg ccgcaagccc
    11461 ttggtgttgc cgctgtagcc ggtgtggccc tggtaaccat cctgtctgtt gtcagaaggc
    11521 gccggtgctg gccttccgcg ttctcgggag cactaggggg ttgtgtatcg gctctggttt
    11581 tcggctgggg ctcaatattc atgcttcgcc tttccgggct ttctacggcc acggcatccc
    11641 ttctcgcaag tactgtcccg ggaatcgacc tcaggggcat acttcttgca tccataatta
    11701 tcggggcttc aggggcggtg ctggatgtgg cggtttctgt aacttcggcc atggcggaga
    11761 tgaaggatca cgatcccggc attgaggcca gggtactttt ccagtcaggt attaacgtgg
    11821 gaaaggaagt cctgggaagc atggtcaata cactggtctt cgcctacttc ggcaactccc
    11881 ttgtcctctc gctgctgatc ctggaggctg aacccatacc catagcgatg ctcaacgatt
    11941 ctgtcatcgc cgaagaggtc ataagggcac tggccggaac ggctgggctg cttctaacca
    12001 ttcccctgac ggcccttttc ggggccctga tgatgtcagg cgggtccgtg cagagcgggg
    12061 aaggataagt ttctgcagaa ctttggcagg gctcttttcg cgagtatgct gttatgaggg
    12121 atctctgaag aatggaaagc ggagcccttc atgtgagagg gccccgcgcc ttttgtgttg
    12181 atgggaaatc ggtcctagaa cggccccagc ttgataaagt gtgcgatggt gaccaggatc
    12241 gcccccagca ggtactgaag aagaatgagc ggaaggaacc atttcgccca acggtcccag
    12301 ggtatcttgg ccagagcaag accagccata aaataccccg aagtgggtgt gaagatattt
    12361 gatattccgt cgccgaactg gtatgcaagg accgcgacct gccttgtgac gcctacaagg
    12421 tcccccagcg gggccatgat aggcatggat acagccgcct ggccgctgcc tgaggggaca
    12481 atgtagttga gcaggcactg gaatacgtac atccccatgg ccgagaatat tgaggggaga
    12541 gaggcgacgg ccgtcgctgc accgtagagg atcgtgtcca gaatgttgcc ctgggtcagc
    12601 acaaccagga tagcccttgc aaacccgacg accagggctc cagtggtgag agcggccatc
    12661 ccgtcagcca ggttgatcgc gaacccgttc agtccaagtt tgccgacgat ccctaccagg
    12721 atagccatcc ccaggaaaag tccggagatc tcctggatgt accacccgta tttcataacg
    12781 ccgaaaacca ggagaaccat cgtaccggca agtaccagaa gaacaagctg gtgagatgcc
    12841 gtgaactctt tcatgacatt caggtcaagg acctcttccc gggtcttgtc gatctcgtac
    12901 atgaggctcc gggcgggatc ttttttgacc cgtcccgcat accagtagat gaaagctact
    12961 gtcagagcca gcattgagat gtagcagaag atccggtacc ccatacccga gaaaaggggc
    13021 agttcagcga tgccttgcgc aacccccacg gtgaaggggt tcatgaaggc agcggcaaat
    13081 cctgcgcctg ctccagcaag gacgatgcct gcgccggtca gggaatcaaa gccaa
//
LOCUS       DAOTIQ010000052        12892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5799_length_12892_cov_9.143102, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000052 DAOTIQ010000000
VERSION     DAOTIQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_12892_cov_9.143102"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1181)
                     /locus_tag="P5541_07100"
     CDS             complement(204..1181)
                     /locus_tag="P5541_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRV71788.1"
                     /translation="MKIDPQMSDVLLAARFLKGRIRHTPTERSEPLSRLSGGEIFLKW
                     ENHQICGSFKIRGALNKMFSLSLEERGKGVVTASSGNHAQGIAMAARSLGVRAVICVP
                     GVCPQTKRDAILMLGGESVELRVVGHLYDDAEDEAHRLQKEEGMTYVSSYEDHFIVSG
                     AGTLGLELIMDEPEIDMVISPAGGGGLLNGVAIPVKTARPEAEIWGVQSVASNPYVVS
                     WPGGKVLDVEYKDSLADGLTGSIPQSLLDLAKKRVTGFVEVQESDIAQAIGFLHSKHH
                     QVVEGAGAVGVAAILAGKIPVKGKRVGVVISGGNIDDGRLLQILNDYRE"
     gene            1362..1856
                     /locus_tag="P5541_07105"
     CDS             1362..1856
                     /locus_tag="P5541_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71789.1"
                     /translation="MKKAISLLAVLFAFTAITAPRLDASTSLNNANALSLYMYKGQVQ
                     SYVGYPDSVEYNSNYRRNLEFYATPDGYDVYQVFTGYMSNGQLGVLGNVFRPGRTTLM
                     ALANQLRGKGLTQIASDGRSFLFQGKDPRTGRLMYAIVEDWTSSGLGPVLTLLTEEVY
                     RQIM"
     gene            complement(1849..3024)
                     /locus_tag="P5541_07110"
     CDS             complement(1849..3024)
                     /locus_tag="P5541_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV71790.1"
                     /translation="MSIYLNNGATTWPKPPGVAEAVSGFITGRGANLARGSSSGRDID
                     TLDLVTSTRMKLADFFGGYSKADPRYVTFTSNVTESLNVVLKGYLRPGMHAATTSMEH
                     NSVIRPLRRLESEGVKVTVLECDNRGLLDPADLESLFRSQKVDLFVFSHASNVCGTIQ
                     PLEDIASICRSAGVPCVLDSAQTAGVVPIDASGLGLAAMCFTGHKGLMGPQGIGGIVW
                     APEFAKTVSTFIEGGTGSFSDEEIHPVKMPDRFEAGTPNLPGTAGLFAAIDWISSTGV
                     AKISEREKGLGRRLYEGIRGLKGLKIYGMDDERNRLAVYGMNFSRIDNGTAAMELSER
                     WGIETRPGLHCAPRAHRTLGSFPAGALRISVGYFNTEEDIDTAVKALESLARENPAT"
     gene            complement(3417..4532)
                     /locus_tag="P5541_07115"
     CDS             complement(3417..4532)
                     /locus_tag="P5541_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV71791.1"
                     /translation="MPFRAAILYASVGTGHKTAALALEKWFGLEFPGSKVLCLDTLSF
                     SSPIVRGVYTRSYLEMVRRIPHLWGYFYDLTDEPDARSGVIATLGELTEKLNIQKLRK
                     TLIDFSPDAVIFTHFFGAAALAGEFTPEVPVYYVNTDFLSHVFHRNPSFSGWFVPTEE
                     TLSQHREDGLPRERVFLTGIPVDPSYASLPDKREARELLGLPEKEKTALVMGGGIGMG
                     SMEAVVSSLADGGFIVHALCGNNEKIRKRLLEKYDNHGRVTVCGFVDEMPLRFVSADI
                     IFMKPGGLSSSEALCTGTPVVITDPIPGQEQRNSDYLLERGAVRVLLDYRLSAEKAQK
                     ILGSKTEAALMRRAALKIARPLAGKEIVRKIVDIEGQ"
     gene            complement(4538..5551)
                     /locus_tag="P5541_07120"
     CDS             complement(4538..5551)
                     /locus_tag="P5541_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRV71792.1"
                     /translation="MSRAGLHIISHTDLDGVVAAAVAWYWNFPSRSPLRVSLTGYGEV
                     DFLVLESAARGEPFLVLDLFCQKPETIDNIDSFFPSGEEPLFFDHHQSTIDRYGSRPW
                     VFADSRFCAAKVYHDWLMEKAAPGKERERIEPLKEIVEVANDRDLWLNRIPESRLWQA
                     LITLCGPWSVFGRIIRDPAAALSGSERSFATSFVEEQEERFRKALAASARTGDDLLFV
                     EPGNLEFGDVSDFCGLVLDRMESPPRVAAVLSRRPAGDWNVSLRSRDGFAGRVTGLLR
                     DGKKVRGGGHDDAAALYFPPQFKAGQIRDSIVSAVRSAVESDAPSGLSLGDVLREAMN
                     KGR"
     gene            5839..7161
                     /gene="dnaA"
                     /locus_tag="P5541_07125"
     CDS             5839..7161
                     /gene="dnaA"
                     /locus_tag="P5541_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRV71793.1"
                     /translation="MEETITKTWQKILEIAEENLPKGAADIWLKTCLPVSLTDMVLTL
                     DVPNVFVREQIQSRFIERLIEITSRQGLADKIVLEVGGGAKKDEIRRAEKITRETERP
                     KNGLNPDYQFDSFVVGKSNRLAHAASLAVAESPGVAYNPLFFWGGVGLGKTHLLHAIG
                     NFVTKHNEGLKISYISSEKFINEFIMSIQNNKTHEFKVKYRSVDVLLIDDIHFLANKE
                     STQEEFFHTFNSLHDAKKQIVLTSDKPPKDIHNIEERLVSRFEWGLVTDIQPPDLETR
                     MAILRKKAAIKNYYVPDDVIDFLAQNIPSNIRELEGSLNSVMFFADLNNESITVDNAS
                     RWLKDLIRRSSRGQVTIDLIQQITAEAFGIPVSALSGNKRTSEIALARQVAMFLSRDL
                     TGTSLQQIGYAFNRKDHTTVIHACKKIEEGCKKDQRLKNMVDNIREKL"
     gene            7382..8524
                     /gene="dnaN"
                     /locus_tag="P5541_07130"
     CDS             7382..8524
                     /gene="dnaN"
                     /locus_tag="P5541_07130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRV71794.1"
                     /translation="MKLLVDKTKFLKSWSLTERGAGHRSTISVLSGVKCEAGPGGVVL
                     KATDLKTSISCHAEGVKVEEPGSVIFPVKIIGEFFKKAPGESFTVEVKGGKATMVSGS
                     SVYKFSTYPVSEFPSLPTSANGQPFFRSLVGDMRRILEEGTFSGSPGEEFPQYLSAGL
                     IQTEESKVRVVSTDGRRLSLSQGEIKIEGEPDQVLLPLSGLRELQRALATVEAEQEIV
                     VSRDDSQVFFVTDGIEFSVRRVESRFPPYEKVLSSERTTWITINRETFIEALERTEVI
                     VRDFSRMVVFSLSPGGVLKLQAKAPEVGEAFEEIEGDCDGEVLKIAFNVKYLLEGLKA
                     LHGSSAHLSFNGSNGQMTLSRPGEDSFMYVLMPITLPEEGSAEEIQ"
     gene            8521..9576
                     /gene="recF"
                     /locus_tag="P5541_07135"
     CDS             8521..9576
                     /gene="recF"
                     /locus_tag="P5541_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRV71795.1"
                     /translation="MKINISHWEGFRNLKPRRLEWEEGLHLLLGPNGSGKTNILEAFH
                     IMTGWGSLRGERVSDLKRWEGTERGTFLSGEFCGEEEFILRVGIYNRILMKKGNDPIR
                     ASQLRPLVPSLLFLPGDLSLVEGSPATRRRLLDRLCALLYPLYAFRLHEYGKALKQRN
                     ASLKAGRNQTATARVMEPLATWIWACRLAATDLIGNGLERPDSFLPRGVKVELKRGGM
                     GLEDLHEDFWESLKASRSRESATFSCPVGPHRDDLIIKFGEREASRCFSRGYRRRIAV
                     ALLLSSGRAVERKTGRKPVLLLDEVMAELDLEGRSGLIETLLGTGWQVFATTAEICTP
                     QWPGKIWKVENGDISVS"
     gene            9589..11496
                     /gene="mnmG"
                     /locus_tag="P5541_07140"
     CDS             9589..11496
                     /gene="mnmG"
                     /locus_tag="P5541_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRV71796.1"
                     /translation="MEGQLLEDRIFDVIVVGGGHAGCEAALASSRMGSRTLLLNLLLD
                     NAAMMPCNPSIGGPAKGHLTREIDALGGEQAMAADGSTIHIRVLNTSKGAAVRTLRAQ
                     CDLWDYHRWYRRVCDEQENLVVIQEEVTDIWVEEKMIRGVKTRIGSIYEAKKVIIATG
                     TFLGGRVHIGLSNFGSGPLGQIPATELSKSLLETGITVGRLKTGTTPRIHAETVDWAS
                     LERQDSAPEPLCFSHWGTPRIYSGYACHATRTTSLTHEIINGSLDRSPLFTGAIEGKG
                     PRYCPSIEDRVVRFPDKESHLVFLEPVARGSCEIYMQNFTTSLPLDTQVRMVRSLPGC
                     EKARIMRPGYAIEYDFIPPTQLRPWLESREIKGLYFAGQTNGTSGYEEAAAQGILAGI
                     NASLACRGEEPLVIERSRGYMGVLVDDLVTRGTTEPYRMLTSRCEFRLLLRHDNADRR
                     LAPLGRDLGLIGNERWKMLQARWKEIDEEIERLKKTRVPRGIETEKLLEEAGAGSFVP
                     GSTAAEILKRPGVTWEMIARIAPPGQELSPEATERATIEIKYEGYISRQISQVDRMRR
                     MDQVRIPEELDYGSIRGLLNESRQKLAAARPSTLGQAGRISGVTPADIMLIEVYMEQR
                     RREAKNGRQER"
     gene            11477..12016
                     /locus_tag="P5541_07145"
     CDS             11477..12016
                     /locus_tag="P5541_07145"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRV71797.1"
                     /translation="MAVRRGRRKGEVSAVGSLLLSSLSKEQSRMLLLADLATRWEEVV
                     GPNRGGKSSPGSLVDGNLVVTAVSPSCAQDIRMRAGDLVLKIREKWGLDVSSITVRVG
                     KVKKDRHLPGKARKSSVVLPEPESIDECREKIRGVIPREDVEEGLARLMAQYRKRFGG
                     GRAGEDRGENGNGWRKKIR"
     gene            11991..12737
                     /locus_tag="P5541_07150"
     CDS             11991..12737
                     /locus_tag="P5541_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRV71798.1"
                     /translation="MDGERRSADLGLSVLGVALAVGIVFSSILVSSTIKDVKMANQTI
                     VVKGYAQKEVKSDIAVWNGRFTSRDPDLVTAYARLERDLKVVVDYMARSGIPEENISV
                     SAVSTRIQNRRTPQGYETNEIEQYVLDQTVSVSSNNVDLVSSLSRESTILIREGIEFF
                     SYSPEYYYSKIEDMKIQLLGEATLNARQRAEQLAVNSGGKVGPLRAATQGVFQITPVY
                     STDVDDWGRYDTTTINKAVKAVVTIQYSID"
BASE COUNT         3359 a         3170 c         3379 g         2984 t
ORIGIN      
        1 cccttccagt ttccagtctc ctgtttcctg ccgcctttgt ttcccgttca ccgtttacgc
       61 ccttgaccct gctgtcatcc tgagcgcagc gaaggatctg tttttgtcct ttgatttttt
      121 cgtctaacgt ctaacgtcta acgtctcacg cccttgccct tccccttcac cgttcaccgt
      181 tcacgccttt aaggttttcc gattcactcc ctgtagtcgt tgagtatctg gagcaacctc
      241 ccgtcgtcta tgttgccacc ggagataact actcccacac gtttgccctt tacagggatc
      301 ttcccggcga ggatggccgc taccccgact gccccggcgc cttcaacgac ctggtgatgt
      361 tttgagtgga gaaaaccgat ggcctgggca atatctgatt cctggacttc gacaaacccg
      421 gtcactctct ttttcgcaag atccagcaaa ctctgaggta tcgaaccggt aaggccgtcg
      481 gcgagggagt ctttgtactc cacatcaagg accttgcccc ccggccagga aaccacatag
      541 gggttggacg cgacggactg cactccccag atctccgctt ccggcctggc agttttgacg
      601 ggaatggcaa ccccgttcag tagacctccg ccacctgcag gagaaatgac catatcgatt
      661 tctggttcat ccatgatcag ttcaagaccc agagtaccgg ccccactcac gataaagtgg
      721 tcctcgtagg aagagacata ggtcatcccc tcctcttttt gaaggcgatg tgcctcgtcc
      781 tcggcatcgt cgtaaaggtg tcccactact cttaattcaa cggattcacc acccagcatc
      841 aaaatggcat ctctcttcgt ctgagggcat accccaggaa cgcagatcac tgccctgacg
      901 cccaacgacc tggctgccat agcgataccc tgtgcatggt tgccgctgga tgctgtcacc
      961 acaccctttc ccctttcttc cagggaaagg ctgaacatct tgttgagggc acccctgatc
     1021 ttgaaagagc cgcagatctg atggttctcc catttcaaaa atatttcccc accggaaaga
     1081 cggctcaaag gttcggatct ttccgtggga gtgtgtctga tacgcccctt caggaaacgc
     1141 gcggcgagaa gaacatcaga catttgagga tctatcttca tatttagcac ctcctggcat
     1201 ttttcaatat tcagtataca tgaagcgggc ctgtcggggt aaattcagga tcgggaaacc
     1261 atttttgcag ttctttcctt gtcagaactt tctctcggta atacaataaa tttaggatat
     1321 gaaaggtgca aaaattttat atatatggag gatataagag catgaaaaaa gccatatcgt
     1381 tactggctgt tctgttcgca tttacagcaa taacggctcc gcgcctggat gcatcgactt
     1441 ccctcaacaa cgcgaacgcc ctgagcctat acatgtacaa ggggcaggtg cagagttacg
     1501 tcgggtaccc tgattctgtg gaatacaaca gcaactacag acgcaatctg gaattttacg
     1561 ctacccccga cggctacgat gtatatcagg tgtttaccgg ctatatgtcc aacgggcagc
     1621 ttggggttct gggaaatgtt ttcagacctg gcaggacaac acttatggct ttggcaaacc
     1681 agttgcgggg gaaggggctg acacagatag cgtccgatgg gagatctttc ctctttcagg
     1741 gaaaagaccc ccgcaccgga cgcctgatgt acgcgatagt cgaagactgg acgtcctctg
     1801 gtctcggacc tgttctgact cttctgaccg aagaggttta caggcagatc atgtagcagg
     1861 gttctccctt gccagggatt caagggcctt tacagcggtg tcgatatctt cctcggtgtt
     1921 gaagtaaccg acgctgatcc tgagggcccc tgccgggaag cttccaagtg tccggtgggc
     1981 cctcggggca cagtgaagcc ccggccgagt ctctattccc catctttcag aaagttccat
     2041 ggccgctgta ccgttatcga tccgggaaaa gttcatccca tagacggcaa gccggttacg
     2101 ttcgtcatcc ataccgtata tcttcaaacc cttcagaccc ctgatacctt catacagcct
     2161 tctcccaagt cctttttctc tttcggaaat ttttgctacg ccggtggagg atatccagtc
     2221 aatggcagca aaaagtccgg cggtacctgg aaggttgggc gtccctgcct caaagcgatc
     2281 gggcattttt acgggatgga tctcttcgtc cgaaaaactg cccgttcctc cctcaatgaa
     2341 tgtcgagact gtttttgcaa attcgggagc ccagactatc ccgcctatgc cctggggccc
     2401 cataagtccc ttgtgcccgg taaaacacat ggccgcaagg ccgaggcccg atgcgtcgat
     2461 cgggacaacc ccggcggtct gggcggaatc aaggacacag ggaaccccgg ccgaccggca
     2521 gatggatgcg atatcctcca gtggctggat agtcccgcaa acgttacttg cgtgggagaa
     2581 gacaaagagg tcaacctttt ggcttctgaa cagagattcg agatccgcag ggtcgagaag
     2641 gccccgattg tcacattcaa gcacagtgac cttgactcct tcgctttcca gcctcctgag
     2701 gggcctgata accgagttgt gctccatact ggtggttgcg gcgtgcatcc cgggtctcag
     2761 gtaacccttt agaaccacgt tcagggattc cgttacattg gaggtgaaag ttacgtatct
     2821 ggggtcagcc ttgctgtagc ccccgaaaaa atccgcaagt ttcatccttg tggaggtcac
     2881 gagatcaaga gtatcgatat cgcgccccga tgatgaaccc cttgcaaggt tggcaccccg
     2941 tcccgtgatg aaaccgctga ccgcctcggc aacaccgggc ggtttgggcc aggttgtggc
     3001 cccgttgttc aggtagatcg acaatgccca tcactctcct tttcgatatt ggatatcatt
     3061 ttgatgatat ccgaccctac caaggatatt ccatcaacat aggtaataca accgtttact
     3121 ctatgccctg atggggtatg tttaagctgt acagcatcat tttttgggag gtggtatcgc
     3181 aatgaagtct tcaaattctc cgtttattgg ttcagtttgg cttcctggca tttatgacat
     3241 ggctcggcta cagacaccag ttgcttggag ggagccctga aggaagccgg ttggcccacg
     3301 gacacgagcc ccgacacctc gatcaagagc ctttcggatg aattggggaa aagccccaat
     3361 gacataagat aggcagtgaa aaaaatgtca aaataagagc atggcaacgt tgtcttttac
     3421 tgaccttcaa tatccactat ctttctgaca atttctttgc cggccagggg tctggcaatt
     3481 ttcagggcgg cccttctcat gagcgccgct tctgttttcg aaccaaggat cttttgtgct
     3541 ttttccgctg aaagcctgta gtcgagcaac accctgaccg ctccacgctc aagaagataa
     3601 tcgctgttcc gctgttcctg tccaggtatg ggatctgtta ttacaacagg ggtgcctgta
     3661 cacagggctt cggatgatga aagtccgccg ggtttcatga atataatgtc ggccgaaaca
     3721 aaacggagtg gcatctcgtc aacaaatccg caaactgtaa ccctgccgtg gttgtcgtat
     3781 ttctccagga gccttttcct gatcttctca ttgttcccgc agagggcgtg gacgatgaag
     3841 cctccgtctg ccagtgaaga gacgacggcc tccatggagc ccatccctat acccccaccc
     3901 ataacaagag ctgttttttc tttttcgggt aacccgagaa gttcccttgc ttctctttta
     3961 tcaggtaaag aggcatagga ggggtcgaca ggtatgccgg tcaaaaaaac cctttcccgt
     4021 ggcagtccat cttcacgatg ctgcgacaga gtctcctccg tcggaacgaa ccagcccgaa
     4081 aaggaagggt tgcggtggaa aacgtggctg agaaaatcgg tattcacata ataaacgggg
     4141 acctcgggag tgaactcccc ggcaagagca gcggctccaa aaaaatgggt gaaaataacg
     4201 gcatccgggg aaaaatcgat gagggtcttc ctgagtttct gaatgttcag tttttctgtc
     4261 aactctccga gtgtggcgat aactccgctt ctcgcatcgg gttcgtctgt gagatcatag
     4321 aaatatcccc agagatgggg gatccttctc accatttcca ggtaagatct cgtataaacc
     4381 cctctcacta tcggggaaga gaaagataga gtgtccagac aaaggacttt actcccggga
     4441 aactccaggc cgaaccactt ttccagggcc agggcagcag tcttgtgacc tgtcccgacc
     4501 gaggcgtata gaattgcggc acgaaagggc atttctctta cctccccttg ttcatggcct
     4561 ccctgagaac atcgccgaga gaaagtcccg agggagcatc ggattccacc gccgatctga
     4621 cagcggacac tatggaatcc ctgatctgcc cagccttgaa ttgcggagga aaataaaggg
     4681 ccgccgcgtc atcgtgcccg ccgccacgga cctttttccc atccctgaga agtcctgtca
     4741 cccttcccgc gaaaccgtcc cggctcctga gggaaacgtt ccagtctccg gcgggacgcc
     4801 ggctcagaac agcggcaaca cggggagggg actccattct gtccagcaca agcccgcaaa
     4861 aatccgagac gtcgccaaac tcgaggtttc caggctcaac gaaaagcagg tcgtccccgg
     4921 ttctggcaga ggcggcaagg gcttttctga atctttcctc ctgctcctct acaaaagagg
     4981 tggcaaaaga tcgttctgaa ccggaaaggg cagcggccgg atcacgaatg atccgcccaa
     5041 aaaccgacca gggaccgcaa agtgttataa gcgcctgcca gagacggctc tctggaatcc
     5101 ggttcagcca gaggtccctg tcgttggcaa cttcaactat ttctttaagg ggttctatcc
     5161 tttctctttc tttcccggga gccgcttttt ccatcagcca gtcatggtaa accttcgctg
     5221 cacaaaaccg gctatcagca aaaacccagg gtctgcttcc gtacctgtct atggtgctct
     5281 ggtgatggtc aaagaataaa ggctcttctc cggatgggaa aaaactgtca atattatcta
     5341 tcgtctccgg tttctggcag aaaagatcca gaacaagaaa gggttccccc ctggcggcag
     5401 attccaaaac aagaaaatca acctcaccgt accctgtcag ggaaactctc aaagggctgc
     5461 gggatggaaa gttccagtac catgcaactg ctgctgcaac aacaccgtca agatcggtgt
     5521 gactgattat gtgcaaaccg gctctgctca ttattgatct cctttttatg gagttgttta
     5581 tgattataga acatgtccgg agttgggata tgtgaataag attgtgaata actttgtaga
     5641 gcccagtggt aggtttcgtt atctttggtc aaattcattc aatttacagt ctttttcttg
     5701 caacccctca aaacagaggt tttctccggc acccaaacaa agaggacaaa atgtggatat
     5761 gtggataaca tttgtggatt catctatggt aacattgtgg ccgaaaccat tcgtattcta
     5821 aaagagggtt gaaagatttt ggaagaaact atcactaaaa cgtggcagaa aattctcgaa
     5881 atagccgagg aaaacctccc taagggagcc gcagacatct ggctcaagac ctgtcttccc
     5941 gtatccctga ccgacatggt ccttactctg gatgtcccca atgtattcgt ccgcgaacag
     6001 atccagtctc gtttcatcga aagacttata gaaattactt cccgccaggg cctggctgat
     6061 aaaatcgtcc tggaagtcgg tggaggggca aagaaggacg agatacgcag ggccgaaaaa
     6121 attacccggg aaaccgaacg cccaaaaaac ggtcttaatc ccgactacca gttcgactct
     6181 ttcgtcgtag gaaaatccaa caggctggct cacgccgcaa gcctcgctgt ggccgagtcg
     6241 ccgggtgttg cctataaccc cctctttttc tggggtgggg tcggacttgg aaaaactcat
     6301 cttcttcacg caatagggaa ttttgtgaca aaacataacg aaggtctgaa aatttcctac
     6361 atcagttccg aaaaattcat aaacgagttc atcatgtcca tccagaacaa caaaacccac
     6421 gaattcaagg tcaaatacag aagcgtcgat gtgcttctca tagacgacat tcattttctg
     6481 gcgaataaag agagcaccca ggaggaattt tttcatacct tcaacagcct gcacgacgcc
     6541 aagaaacaga tagtcctgac atccgacaaa ccaccaaagg atattcacaa catagaggaa
     6601 aggcttgtaa gccgttttga gtggggactt gtaacagata tccagccccc cgatctggag
     6661 acaagaatgg ctatcctgcg caaaaaagcg gccatcaaaa actattatgt tccggacgat
     6721 gtcatagatt tcctggctca gaacataccc agcaacatca gggaactgga aggatcttta
     6781 aacagcgtga tgttcttcgc cgatctgaac aacgaatcca ttactgtgga caatgcttca
     6841 agatggctaa aggatcttat tcgcaggtct tcaagggggc aggtaaccat agatctgatc
     6901 cagcaaataa ccgccgaggc ttttggtatt cccgtatcgg ccctctccgg taataaacgg
     6961 acttcagaaa tcgcacttgc aaggcaggtg gccatgttcc tgagcaggga tctgacagga
     7021 acaagcctcc agcaaatagg ttatgccttc aacagaaagg atcacacgac agtaatccac
     7081 gcatgtaaaa aaatagagga aggatgcaaa aaggatcaaa gattgaagaa tatggtggat
     7141 aatatcaggg aaaagttgtg attcaactgt ggattcattt gaaaccatcc acagtgtaaa
     7201 gttcctgttc ttttattctc ctgtgttgtg gaaaggtatc cacaaaaaat ttctttctta
     7261 tacacagtaa aagggcaaag gtttagcctt tttttatctt ttccacaaaa aacacaccat
     7321 tattactaga actactgctt ttttatttct ataacataaa tagagaaggg gtgactatga
     7381 attgaaactt ctcgtggata agacaaaatt tcttaaaagc tggtccctta ctgaaagagg
     7441 tgccggtcac agaagtacca tatctgttct ttcaggtgtc aaatgtgagg caggaccagg
     7501 gggtgtggtt ctaaaggcca cggatcttaa aacctctatt tcctgccatg cggaaggggt
     7561 aaaggttgaa gaaccgggaa gcgtaatatt tcctgtcaag ataattgggg aattttttaa
     7621 gaaggctcca ggtgaatctt ttacagtaga ggtaaaaggc ggaaaggcta ccatggtttc
     7681 cggaagcagc gtctataaat tttcaacata tccggtatct gaatttccca gccttccgac
     7741 ctccgccaat gggcagcctt ttttccgatc acttgttgga gatatgagaa gaattcttga
     7801 ggagggaacg ttctccggtt cgccgggaga ggaatttccc caatatttga gtgccggcct
     7861 catacagaca gaggaaagta aagttagagt tgtttcaaca gacggaagga gactttctct
     7921 ttcgcaggga gaaataaaga tagaaggtga accggatcag gttcttcttc ccctttcggg
     7981 actcagggaa ctacagagag cccttgccac ggttgaagca gaacaggaaa ttgttgtatc
     8041 cagagatgat tcacaggtat tttttgttac cgatggtatt gagttttccg tcagaagagt
     8101 ggaatcccgt tttcctccat acgaaaaagt tttgtcctct gaaaggacca catggataac
     8161 gatcaacagg gaaaccttta ttgaagccct ggaaaggacc gaagtaatag tcagggattt
     8221 cagcagaatg gtggtttttt cactttctcc aggaggagtc ctgaaattgc aggcaaaggc
     8281 tccggaggtg ggtgaggcat ttgaggaaat cgaaggagac tgcgatggag aagttctcaa
     8341 aatagccttt aatgtcaaat acctgctgga gggactaaag gctctgcacg gaagttcggc
     8401 acatctctcc tttaacggat caaacggcca gatgactctt tccaggccgg gagaagacag
     8461 tttcatgtat gttctcatgc ctatcacact ccccgaggag ggctccgcgg aagaaataca
     8521 atgaaaatca atatatctca ttgggaagga ttcagaaacc tgaagccccg ccgccttgaa
     8581 tgggaggagg ggcttcatct gcttctgggc cccaacggat cgggaaaaac caacattctc
     8641 gaggcttttc acataatgac agggtggggt tccctgcggg gagagagggt ctcggacctt
     8701 aaaaggtggg aggggacaga aaggggaacc tttctgtcag gagagttctg cggagaagaa
     8761 gaatttatat taagggtcgg aatatataac aggatattga tgaagaaggg aaacgatcct
     8821 atcagggcgt cgcagttgcg acctcttgtt ccgtcactgt tatttttgcc gggagatctg
     8881 tccctggtag aaggttctcc cgccacaaga cgaagattgc tggatcgcct ttgtgcttta
     8941 ttgtatccac tttatgcctt ccggcttcat gaatacggaa aggcgctgaa acaaagaaat
     9001 gcttcattaa aggcagggag aaatcaaacg gctacggcga gggttatgga acccctcgca
     9061 acatggatat gggcatgccg ccttgcggcc acagatctta ttgggaatgg ccttgaaaga
     9121 ccagattcat ttcttccccg gggagtaaag gtcgaactca aaaggggagg gatgggactt
     9181 gaagatcttc atgaagattt ctgggagtcc cttaaagcat cccgttccag agagtcggca
     9241 actttttcat gccctgttgg accacaccgg gatgatctga tcataaaatt tggagagagg
     9301 gaggcctccc gctgcttcag ccggggctac cgcagaagga tcgccgttgc gctgctgctt
     9361 tcttcaggga gggccgttga aaggaaaacc gggagaaaac ctgtccttct tctcgatgag
     9421 gtaatggctg aactggacct tgagggcagg tcggggctta tagaaaccct tcttggaacg
     9481 ggctggcagg tttttgccac gaccgcggaa atatgcacgc cgcaatggcc gggaaaaata
     9541 tggaaggtcg agaatggaga tatatccgta tcatgatatt tgggttctat ggagggacag
     9601 ttattggaag accggatatt tgatgtgatc gttgttggag ggggacatgc tgggtgcgag
     9661 gcagccctcg cgtcgtcccg aatgggttca agaactttgc ttctaaacct gctcctggac
     9721 aatgcggcaa tgatgccctg caacccttct ataggaggtc cggccaaagg gcatcttacc
     9781 agggagatcg atgcccttgg aggagagcag gccatggctg ccgatggatc caccatccat
     9841 ataagggtac tgaatacatc aaaaggcgcg gcggtcagaa ctcttcgtgc ccaatgcgat
     9901 ctttgggatt accaccgatg gtacaggcgt gtctgcgatg aacaggaaaa cctcgtcgta
     9961 attcaggaag aggttacgga tatctgggtc gaagaaaaaa tgatacgcgg agtaaaaaca
    10021 agaattggca gtatatacga agcgaagaaa gtgataatag cgacggggac cttcctcggg
    10081 gggagggtac atataggcct ttcaaatttc ggatcgggcc cccttggaca gatccctgcc
    10141 acggaactga gcaaaagtct tctggaaacg ggcatcaccg tgggaagact caagaccgga
    10201 acgactccca ggatacatgc cgaaacagtg gactgggcat ctcttgaaag acaggacagc
    10261 gctccagagc ccctttgttt cagccattgg ggaacccccc gtatctacag cggctatgca
    10321 tgccatgcga ccaggactac atcactgaca cacgagatca taaatggctc cctcgacagg
    10381 tcacccctgt tcactggagc catagagggt aaagggccga gatattgccc atcgatagag
    10441 gacagggtgg tgcgctttcc tgacaaggag agccacctgg tatttcttga accggttgcc
    10501 aggggcagtt gcgagatata catgcagaat ttcaccacca gcctgcctct tgatacgcag
    10561 gtcagaatgg tgagatcttt gcccgggtgc gaaaaggcaa ggataatgag gccgggatat
    10621 gcaatagaat acgacttcat acccccaacg cagttgagac catggcttga atcaagggaa
    10681 atcaagggtc tttacttcgc ggggcagacc aacggaacat ctggatacga agaggcggcc
    10741 gcacaaggca ttctcgccgg cataaacgca tcccttgcct gcagggggga ggaacccctg
    10801 gtgatagaaa ggtccagagg ttacatgggg gttcttgtgg atgatctcgt tacaagggga
    10861 actacggaac cttaccggat gctgaccagc cgatgtgagt tccggctgct tctcaggcat
    10921 gacaacgccg acaggaggct ggcccccctg gggcgagacc tcgggctgat cgggaacgaa
    10981 agatggaaga tgctccaggc aagatggaaa gagatcgacg aagagataga aaggctcaaa
    11041 aaaacccgtg tcccccgggg aatagaaaca gaaaaacttc tcgaagaggc gggggcgggg
    11101 tcctttgtcc ctggttcgac ggcagcagaa atcctcaaaa gacccggcgt gacatgggag
    11161 atgatagcca ggatagctcc cccggggcag gaactctcgc ccgaggcaac ggagagggcc
    11221 acgatagaga taaaatacga gggctacata tcaaggcaga tatcccaggt tgacaggatg
    11281 aggcgtatgg atcaggtgcg tatcccggag gaactggact atggttccat tcgtggtctc
    11341 ctgaacgaaa gcaggcagaa gctggccgcc gcaaggcctt ctacactggg ccaggccgga
    11401 aggatctcgg gtgtcacccc cgctgatata atgcttattg aggtgtacat ggaacagagg
    11461 agaagggaag caaaaaatgg ccgtcaggag aggtagaaga aagggagaag tatcagctgt
    11521 cggatctctt cttctttcat ctctttcaaa agaacagtcc cgcatgcttc ttctggcaga
    11581 tcttgcgacc aggtgggaag aggttgttgg ccccaaccgg ggagggaaga gttccccggg
    11641 ttctcttgtt gacgggaacc tggtggtgac agccgtttcg ccttcgtgtg cacaggatat
    11701 caggatgcga gcgggagatc tggtcctgaa gatcagggaa aaatggggtc tggacgtttc
    11761 gtcgataacg gtgagagtcg ggaaggtaaa aaaagatcgg catcttcccg gaaaggcacg
    11821 aaagagttct gtcgtgttac ctgagccgga aagcattgac gagtgcaggg aaaaaatcag
    11881 gggagtcatt cccagggagg acgtcgagga aggactcgcg aggctgatgg cacagtacag
    11941 gaaacggttc ggcggaggcc gggccggaga agacaggggg gaaaacggaa atggatggag
    12001 aaagaagatc cgctgacctt gggctctcgg ttctgggagt tgctctcgct gtgggtattg
    12061 tattttcatc aattcttgtc tcttcgacga taaaagatgt aaaaatggcc aaccagacga
    12121 tcgtggtcaa ggggtacgca cagaaagaag tcaaatcgga tattgccgtc tggaatggtc
    12181 ggtttacctc gagagacccg gacctggtta ccgcctatgc gaggcttgag agggacctga
    12241 aggttgtggt cgattacatg gcccgcagcg gaataccgga ggagaacatc tccgtatctg
    12301 ctgtttcgac caggatccag aaccggagga ccccgcaggg atacgaaacc aacgaaattg
    12361 aacaatacgt tcttgatcag acagtatcgg taagttccaa taatgtggac ctggtctctt
    12421 ccctctccag ggaatccacc atcctcatcc gtgagggaat agagttcttc tcctattcgc
    12481 cggagtacta ttattcgaag atcgaggata tgaaaataca gctgctgggg gaggccaccc
    12541 tcaatgccag gcaaagggca gagcagctcg ccgtcaacag cggaggaaag gttggccccc
    12601 tgagggctgc cacccaggga gtctttcaga taacacctgt ctactcgacg gatgtcgatg
    12661 actggggcag gtacgacacc accaccatta acaaggcagt aaaggcagtg gtcacgatac
    12721 agtactcgat tgattagtga gtcgggaatg ggaaaatccg tgagacgtga gacgagagac
    12781 gaaaaatcaa gggcgtaaac ggtaaacggt aaaacataaa aaacaaggaa gcaggagact
    12841 ggcgtcagga gacaggaaag caaaaccaac tgcgtgaacg gtaaacggta aa
//
LOCUS       DAOTIQ010000053        12621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5948_length_12621_cov_7.070508, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000053 DAOTIQ010000000
VERSION     DAOTIQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5948_length_12621_cov_7.070508"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="P5541_07155"
     CDS             <1..245
                     /locus_tag="P5541_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRV71799.1"
                     /translation="NPLPALVGGAVWYLVIRFSGYVSVGSMVSLATVPVVFLIMGQPA
                     AYFCMSAALALLAVLRHRSNISNLLEGKEGRVGSEE"
     gene            323..802
                     /locus_tag="P5541_07160"
     CDS             323..802
                     /locus_tag="P5541_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRV71800.1"
                     /translation="MSSLTMIIEKHIQEMLETTDEESVFLSRKNLAEHFGCVPSQINY
                     VIRSRFTPERGYIVESQRGGHGYIRVIRLCLDMPEERANHIDELVGDTVNEQEMRRIL
                     ANLQHRKMLTSRERLLVEVAVRFQEEIGRDLLDLSPYKRDSLHAELLRRLLRSLALG"
     gene            814..1350
                     /locus_tag="P5541_07165"
     CDS             814..1350
                     /locus_tag="P5541_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRV71801.1"
                     /translation="MKCERCNEREAEIHIRHQKGKEIEEHHLCRQCAEQMARDGLIPD
                     MTFDFPVESFLGGLFPVHGQPLPDTEEGHAADEGVTCGRCGLDAASFRKTGKWGCPEC
                     FSVFHESVGPLLRKIHGSDVHRGIRPAKACVRESCRGDVDSLRKALREAVEMEEYERA
                     AVLRDKIRETEENNEDGS"
     gene            1334..2407
                     /locus_tag="P5541_07170"
     CDS             1334..2407
                     /locus_tag="P5541_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRV71802.1"
                     /translation="MKTGARDLADAHLGWLDGTGEFADVVISCRVRLARNLSDFVFSW
                     RASPDTLHSIADAFRLRVSNGDLSDFSESLVIDSLDPLARGILVEKHLISPALARGGP
                     GREVAISREGNISVMVNEEDHIRIQAFRDGLGVRDAWEDARKIDQVLEESGYAFDGRS
                     GFLTTCPTNIGTGLRASVMIHLPALSVLRKMGKIVADCGRIGLTVRGMFGEGSDSAGS
                     LFQISNQVTLGQTEEELLDKVAGAASGLVMQERSARTKLLDVKGAELEDKAWRAWGLL
                     RYARSVDRRESLELLSLARMCSGTGILPKVPSGTWNRLLMDVQQAHIQARADRVLDAA
                     ELDRGRADVLRRLFESIPENGAI"
     gene            2426..4912
                     /locus_tag="P5541_07175"
     CDS             2426..4912
                     /locus_tag="P5541_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRV71803.1"
                     /translation="MWQFFTERGKKVIQLAHREALRLGHDVIGTEHILLGLAVEGEGV
                     AAQILSSSAVTIDELRGMVEQYVGRGEQKNKPVDLPLSPRAKKVLDLSMREARNMSVN
                     YVGTEHILLGLISEGEGIAAQMLNTIGITPEKIRSDIAAALSAGDGSSPEAPDGSSDD
                     NSGKGSIRSKTPTLDQLGIALTEMAQRNELDPVIGRSKEIQRLIQILSRRTKNNPVLI
                     GDPGVGKTAIVEGLAQKIIAGDIPEVLRGKRVVQLNVGNLVAGTKYRGEFEERMRKLV
                     KELKDCRDVILFIDEIHTIVGAGGAEGAVDAANILKPSLARGEFQVVGATTLEEYRKN
                     IEKDAALERRFQPVTVEEPNVEDSISILKGLRDRYEAHHRAKITDEALEAAARLSARY
                     ITERHLPDKAIDLIDEAAARARLKTMEAPEDIKEMERRMERLRKEKESFVASQEFEKA
                     ASLRDDERQLVEEIEDRRKEWQRVRNQEEPVVGAHEIAGIVSEWTGIPVLQLTEEESA
                     RLIRMEDEIHKRMVNQSEAVSGVSRAIRRARSGLKDPKRPIGSFLFLGPTGVGKTELA
                     RALSEFLFGSEDAMVRFDMSEYMERHEVAKLIGAPPGYVGYEEGGKLTEALRRRPYSV
                     VLFDEVEKAHPDIFNILLQILEDGRLTDGQGHTVDFRNSVVIMTSNIGAKDVMKGTSL
                     GFAAPGQGESPDWDRVRTGILDSVRKTFRPEFINRVDDMIVFEPLGREDLLKILDLML
                     REVSNRLMERGINIDVSDEARLLLLEKGYDPRYGARPLRRAVQRMIEDRLADMMLEGR
                     ITEDSRIGVSVLDGDLSLVPEESRPISEES"
     gene            4957..5820
                     /locus_tag="P5541_07180"
     CDS             4957..5820
                     /locus_tag="P5541_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRV71804.1"
                     /translation="MFKIDVFWIMLLFFFVFPMAKQWMLERSRVSAIRNLEKKRGSRL
                     ITLIHRQDSAGFLGMFTRNFINIEDSEQVLRAIRLTSSDTPIDLIVHTPGGLLLAASQ
                     IAAALNKHEAKVTVFVPHYAMSGGTLIALAADEIVMDRHAVLGPVDPQIGQYPAVSIL
                     KTVEEKPIAEIDDETLILADISRKAIEQTRKFVVNLLVDNVPREEAERLAQILTEGRW
                     THDYPISSEEAAELGLFVTTDMPTEICEVMGLYPQAGQRRPSVQYVPMPYQGNPGKSS
                     GSGSIQHGRNF"
     gene            5832..6713
                     /locus_tag="P5541_07185"
     CDS             5832..6713
                     /locus_tag="P5541_07185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV71805.1"
                     /translation="MSRFSKRTILLGLVALLVLSSTGLAADRGPVVTVGSETVAEPEM
                     IRLIVEQSGANEMMAPFVLAQLSLEDRNSFADQMVMALLLSEAARTKGLHLDPSVASQ
                     LRWNAVNILAKSYVSSVSGKWDLGRPVLEAWFAEHEKDYATPESAHVRHILVETEAEA
                     TNVLLEVYGRDADFGTVAMKYSKDPGSAQKGGDLGWVFRGQTVGEFDEMAFSLEPGNT
                     GGPVETKFGWHIIQVLDRAPARAPVFDEAINQVREDLQQHYLAMEVERLGGSLGVEVD
                     RDILSTLGGFPAYSATE"
     gene            complement(6764..7612)
                     /locus_tag="P5541_07190"
     CDS             complement(6764..7612)
                     /locus_tag="P5541_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRV71806.1"
                     /translation="MQNGSKGMKIPLFQVDAFSPSPFGGNPAAVCLLEEFPSEATMKN
                     IAMEMNLSETAFVIPRDSGWKDDPDSFSIRWFTPTVEVPLCGHATLAASHVIFSEQEK
                     NGGRISFESASGTLFAELSMKGITLDFPADRPVPAEQPAEVMAAMGITGPEEVLIAKE
                     ALMLLVRVGDEDTVRNLSPDFEAMLRADRGPEKYEGVIVTSPSGRYDFVSRYFGPWEG
                     LNEDPVTGSAHTVLAPYWSGVTGKKDFSAFQASRRGGELHLALSGERVRISGPACVVL
                     RGELQI"
     gene            7710..8372
                     /locus_tag="P5541_07195"
     CDS             7710..8372
                     /locus_tag="P5541_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRV71807.1"
                     /translation="MILERARFDVARYGKIMSEKGLVTGTGGNLSIREGDLVAISPSA
                     VDYSLIMPEDVVVTDMKGVVVEGRLKPSSEIPLHLAVYGSRQDVLSVVHTHSPKACVM
                     ACLGWEIPVFHYLVALAGKKVPVAPYALFGTDGLARNVVDSLGEEKAVLMANHGLLAV
                     GKDLPEAFRLAETVEVVAGIYASVKSLGEPKLLTEAETEALARAFAEYDSVSRGNKPG
                     RI"
     gene            complement(8369..9820)
                     /locus_tag="P5541_07200"
     CDS             complement(8369..9820)
                     /locus_tag="P5541_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV71808.1"
                     /translation="MNASLILAGTAVWFVAGSLLSWHARKKLGQGMSEYFLANRKLGG
                     FVSAMTYSATTYSAFMMVGLVGLTYSSGIGALGFEMTYLAMTVILMVLFAPRYWAAGR
                     IFNLVTPSELLSKRYESPAAGAVSAILCLVMLIPYASVQLMGIGYLFETLSGGTIPFI
                     TGMLIAAAVSLVFSWWAGLRSVAITDALQAVIMLFASLILLYFIAFRLFPEGFSNALA
                     MNSDLLKLKWSFPLFVGLTLPWAFFAVTNPQVVQRLYAPASAANLRKMVLGFSAFGFI
                     YTVLCVVFGLAAAARFPGLDKPDNAMAMLLGSVPVPIALLVTLSIMGAAVSTMNSVIL
                     TLSSMFGRDVVKALAPGITEKSELAICRTLIPVITVACFFFAKMKLGLIAVLSSMASG
                     GLLMQLPAILGVFFWKRATAAGALSSMIAGGLTVGYMYIQGLKPLGHWPPLWGLLLSG
                     TVFVMVSLATTPPKETDSFMGSIRESLESMFGN"
     gene            complement(9817..10023)
                     /locus_tag="P5541_07205"
     CDS             complement(9817..10023)
                     /locus_tag="P5541_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71809.1"
                     /translation="MNSREKIWVIFALVYGTANIAIPFTFLREVASLGGAFMFWNVIT
                     LAVLLAGAWVTSSWSGTPRSGASK"
     gene            complement(10041..10547)
                     /locus_tag="P5541_07210"
     CDS             complement(10041..10547)
                     /locus_tag="P5541_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HRV71810.1"
                     /translation="MLSRENRLRQIVRILEESEEPVSGIHLAEEVDISRQAVVQDIGL
                     LRDRGARIMATSRGYVLRGDSRRRVFAVRHGADEIPEELNTIIEAGGRVLDVVVDHPV
                     YGEIRGNLDLATREDVIRFVNLFKSSGQSPLLSLSNGFHLHTVEAPDEITLDAVENAL
                     SEKGFLEA"
     gene            10668..11282
                     /locus_tag="P5541_07215"
     CDS             10668..11282
                     /locus_tag="P5541_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRV71811.1"
                     /translation="MRDLQKGDAPAFRWSRVAVHEAGHAVMAMLLGLDVVECDITRGR
                     RGRGRSGRCVVVDPDPVGLRRFLVSMGGILAEHLVFGDDRGGGKDREDAGVSLFVYSS
                     KYRQKGSDPGAEAIILEVSELFREQASRTVLMDIASLLDRDGRVDGGRLLVSGNRIGE
                     SLDISGIEKKIADFAEAVPAPGWKDMLLGWISNIKEIIERIRGF"
     gene            11328..12128
                     /locus_tag="P5541_07220"
     CDS             11328..12128
                     /locus_tag="P5541_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRV71812.1"
                     /translation="MRSMIEMEKMNPFEAREKIRLGEWTGPTAGISGGFVQANLVVLP
                     RDWAFDFLLFCQRNPKPCPLLDVTEPGDPVPRLLAPGSDIRTDLPKYRVFRDGILDDE
                     PLDVKSIWRDDLVGFLIGCSFTFEGALMEAGVPVRHVEEGVNVPMFITSIPCRPAGRL
                     SGPMVVSMRPVPPEMVARAVTVTAMYPAVHGAPIHVGSPGSIGITNISRPDFGDPVTV
                     REGETPLFWGCGVTPQAALMASRPPFAITHSPGHMFISDRRDSEYAVF"
BASE COUNT         2908 a         3252 c         3769 g         2692 t
ORIGIN      
        1 ggaatccgct tcccgccctt gtcggcgggg cggtctggta cctggtgatt cgtttctcgg
       61 ggtacgtttc ggtaggttcc atggtatctc ttgcgactgt ccctgtcgtg tttctgatta
      121 tgggccagcc ggcagcatat ttctgcatgt cagcggctct tgccctcctc gctgtgttgc
      181 ggcacaggag caatatttcc aacttgcttg aagggaaaga gggacgcgtc ggaagcgaag
      241 aatagaaggg tttttgtctt gacgaatatt gaccttaacc ctataatttc acttgtcaaa
      301 gaaggtcaaa gggggaaagg tcttgtccag tcttacgatg ataatagaaa agcacatcca
      361 ggaaatgctg gaaacaaccg acgaagagtc cgttttcctt agccggaaga acctcgctga
      421 gcatttcggg tgtgtcccca gccagatcaa ctacgtcatc aggagccgtt ttaccccgga
      481 gcggggttat attgtcgaaa gccagagagg cggccatgga tacataaggg tcattcgctt
      541 gtgtctcgac atgccggaag agagggcgaa ccatatagat gaacttgtcg gggatactgt
      601 caacgagcag gagatgagga ggatactggc caatctccag cacaggaaaa tgttgacatc
      661 ccgggagcgc cttcttgtgg aagtggctgt aaggttccag gaagaaattg gccgtgatct
      721 cctggatctt tctccctaca aacgtgactc cctccacgcc gaacttcttc ggcgtcttct
      781 tcgcagcctt gccctcggat aggatgtgga gccatgaaat gcgagagatg caacgagcgc
      841 gaggcggaaa tccacataag acaccagaag ggcaaggaaa ttgaagagca tcatctctgc
      901 cggcaatgcg cggaacagat ggccagagac gggctcattc ccgatatgac ttttgatttc
      961 cccgtcgaaa gttttctcgg ggggcttttc ccggtgcatg gacaacccct tcccgatacc
     1021 gaagagggac atgccgctga cgaaggagtc acctgcggga ggtgtggtct tgatgccgcg
     1081 tcgttccgca aaacaggcaa gtggggatgt cccgaatgtt tctcggtctt tcacgaaagt
     1141 gtcgggccgc tcctgaggaa gattcacgga tctgatgttc atcgcggcat aaggccggca
     1201 aaggcctgtg tcagggagtc ctgcagaggt gatgtcgatt ccctccggaa ggctcttcgc
     1261 gaggctgtcg agatggaaga gtacgagagg gccgccgttc tgagggacaa aatcagggag
     1321 acagaagaaa acaatgaaga cgggagctag ggatcttgcc gatgctcacc ttggatggct
     1381 tgacggaaca ggtgagtttg cggatgtagt gatttcatgc agggtcaggc ttgccagaaa
     1441 cctgtccgat tttgtgtttt cgtggcgcgc ttcgcctgat actctgcatt ctatcgctga
     1501 tgcctttcgt ctcagggtat cgaatggaga tctttcggat ttttccgaaa gccttgttat
     1561 tgattctctc gatccccttg cgagggggat cctcgtagag aagcatctca taagcccggc
     1621 ccttgcgaga gggggtcccg ggcgcgaggt cgcgatcagc agagaaggga acatctctgt
     1681 catggtcaac gaggaggatc atatccgtat ccaggccttt cgtgacggac ttggggttcg
     1741 cgatgcctgg gaggacgcaa ggaaaataga ccaggttctc gaggagtcgg gttatgcctt
     1801 cgatggcaga agcggtttcc tgaccacctg cccgaccaat atcggtacgg ggctccgggc
     1861 ttcggtaatg atccatctcc ctgcgctttc cgtccttcgc aagatgggta agattgttgc
     1921 cgattgcgga agaattggtc tcacagtcag gggaatgttc ggggaaggca gtgattctgc
     1981 tggttcactg ttccagatat cgaaccaggt aaccctggga cagaccgagg aggaactcct
     2041 ggacaaggta gcgggagcgg cctcggggct ggttatgcag gaaaggtctg ccaggacgaa
     2101 gctccttgac gtaaagggtg cggaactcga ggacaaggca tggagggcct gggggcttct
     2161 ccgttatgcc aggagcgtgg atcgaaggga atccctggaa ctgctttcgc tggccaggat
     2221 gtgttccggg acggggatcc tcccgaaggt tccatcgggg acctggaaca ggcttctcat
     2281 ggatgtgcag caggcccata tccaggcacg ggctgaccgt gtgcttgatg cggcggaact
     2341 ggaccgcggg agggccgatg tgttgagacg gctttttgag agcattcccg aaaacggtgc
     2401 aatttaatac tggaggtatt gccggatgtg gcagtttttt actgaaagag gcaaaaaagt
     2461 aatacagctg gcccacaggg aggccctcag gctggggcat gatgtcatag gtaccgagca
     2521 tatactgctt ggactcgcag tcgagggaga aggagtggcc gcccagatac tcagctcttc
     2581 cgctgtcacc atagacgaac tcaggggaat ggtggagcag tatgttggtc ggggagaaca
     2641 gaaaaacaaa cccgtcgatc tgccgctgag tccaagagcg aaaaaggtgc tggatctctc
     2701 gatgagagag gccaggaata tgtctgtaaa ttacgtcggg accgaacata ttctccttgg
     2761 tctgatctcc gagggtgagg gtatagccgc ccagatgctg aacactatag gaataactcc
     2821 cgaaaaaata aggtcggaca tagccgccgc cctttccgcg ggtgacggat catctcccga
     2881 agcgcctgat ggatcttcgg atgataacag cggcaagggt tccatacgct caaagactcc
     2941 gacccttgat caactgggta tcgctcttac cgagatggcc cagcgaaacg aactcgatcc
     3001 ggtcataggg aggagcaagg agatccagag gcttatccag atactctccc ggcgcaccaa
     3061 gaacaacccg gtccttatag gggacccggg tgtcggcaag acggctattg tcgagggtct
     3121 cgcccagaag ataatagctg gcgacatccc ggaagtcctc agggggaagc gcgtggtgca
     3181 acttaacgtg ggcaacctgg tggccggaac aaaataccgt ggagagttcg aggaacggat
     3241 gaggaaactg gtcaaggaac tgaaagactg ccgtgacgta atactcttca tcgacgagat
     3301 ccataccatt gtgggtgcag gcggggctga gggcgccgtc gatgcggcca atattctcaa
     3361 gcccagcctt gcgagagggg agtttcaggt cgtaggggct accaccctgg aagaatacag
     3421 gaaaaacata gaaaaggacg ccgctctcga aagacgcttc cagccggtaa cggtggagga
     3481 gcctaatgtc gaggactcca taagcatact caagggactc agggacaggt acgaggctca
     3541 ccacagggcc aagataactg acgaggccct ggaagcggca gcgagacttt cggccagata
     3601 tatcaccgaa aggcatcttc ccgacaaggc catcgacctg atcgacgagg cggccgcaag
     3661 agcaaggtta aagactatgg aagctcctga ggacatcaag gaaatggaaa ggcggatgga
     3721 gagactccgc aaggagaagg aatcctttgt agcctcccag gaatttgaga aggcagcatc
     3781 cctcagggac gacgaaagac aacttgttga ggagatagag gaccgccgca aagagtggca
     3841 gagagtcagg aaccaggaag aacccgtagt gggggcccac gaaattgccg gaatcgtctc
     3901 cgaatggacc ggtatccctg ttctgcaact gacagaggag gaatccgcaa gactcattcg
     3961 catggaggat gagatccaca agcgtatggt caaccagagc gaggccgtca gcggtgtctc
     4021 gagggcaata agaagggcga gaagcgggct caaggacccc aaaaggccga tagggagttt
     4081 cctgttcctt ggcccgaccg gggtcggtaa aacggaactt gccagagctc tttcggagtt
     4141 cctcttcgga agcgaggatg ccatggtccg cttcgatatg agcgaatata tggaaaggca
     4201 cgaagtggca aaactgatcg gggctcctcc cggctatgtc ggttacgaag aaggcggaaa
     4261 actgacggaa gccctgagga gacgccccta ttcggtggtt ctttttgacg aggtggaaaa
     4321 ggcacatcct gatattttca acattcttct gcagattctg gaggacggac gccttacgga
     4381 cgggcagggg catacggttg acttcaggaa cagtgtggtc ataatgacca gcaatatcgg
     4441 ggccaaggac gtaatgaagg ggaccagcct gggtttcgcg gctccgggcc agggggaatc
     4501 cccggactgg gaccgggttc gcacaggtat actagattcg gtcaggaaga ccttcaggcc
     4561 cgagttcatc aacagggtgg acgatatgat agtatttgaa ccccttgggc gggaggacct
     4621 gctgaagatc cttgacctta tgcttcggga agtttcgaac cgcctcatgg aacgggggat
     4681 caatatagac gtctccgatg aggcaaggct cctgctgctg gaaaaaggtt acgatcccag
     4741 gtatggggcg aggcccctca ggagggctgt ccagaggatg atagaggatc gcctggcgga
     4801 catgatgctg gaagggcgga tcacggagga tagccgcata ggtgtttccg ttcttgacgg
     4861 tgatctttcg cttgtccccg aagaatcacg cccaatatca gaagagagct gacccgggat
     4921 cttctatccg gcagtaaagt gacgggagtg actgaaatgt tcaagatcga tgtcttctgg
     4981 ataatgctac ttttcttctt cgttttcccg atggcaaagc agtggatgct ggagaggtcc
     5041 agggtttcag ccataagaaa tctcgaaaag aaaaggggca gccgccttat tacgctgata
     5101 cacaggcagg acagcgccgg ttttctgggg atgttcacga ggaacttcat caatatcgag
     5161 gattcggaac aggtacttag ggccataagg ctgacctcct cggacactcc catagacctg
     5221 atagtccata ctcccggggg gctcctcctg gcagcgtccc agatcgccgc ggcccttaac
     5281 aagcatgagg cgaaagtaac agttttcgtg ccccattacg cgatgtccgg cggaaccctg
     5341 atagcactgg ccgccgatga gatagtcatg gaccgtcatg ctgtccttgg ccccgtcgat
     5401 ccccagatcg gtcagtatcc tgccgtctcg attctcaaaa ccgtagaaga aaaacctatt
     5461 gccgagatcg acgacgagac ccttatactt gcggacattt ccaggaaggc catagaacag
     5521 accaggaaat tcgtagtcaa tcttcttgtc gataatgtgc ccagggaaga ggccgaacgt
     5581 ctcgcccaga tcctgaccga agggcgatgg acccacgatt accccataag cagtgaggag
     5641 gcggccgaac tgggcctttt cgtgaccacc gatatgccca ccgaaatatg cgaggtcatg
     5701 ggtctttacc cgcaggcggg acagcgcagg ccctcggtac agtacgtccc catgccctac
     5761 cagggaaatc cggggaagtc atccggcagt ggctccattc agcacggccg gaatttttaa
     5821 ggagtgatag aatgagccgt ttcagcaaga gaacgatcct tctgggtctg gtagcgcttc
     5881 tggtcctttc ttcaacgggt ctggcagcag acagaggccc tgtggtaacg gttggcagcg
     5941 agacagttgc cgagcctgaa atgatccgcc ttattgtgga gcagtcaggg gccaacgaaa
     6001 tgatggctcc cttcgtgctt gcccaactgt ccctggagga caggaattcc ttcgcggacc
     6061 agatggttat ggcccttctg ctttcggaag cggcaaggac aaaggggctc cacctcgatc
     6121 cgtcggttgc ctcgcaatta cgttggaacg ccgtgaacat tcttgccaag tcctatgttt
     6181 ccagtgtatc gggcaaatgg gatcttggca ggccggtcct tgaggcatgg tttgctgaac
     6241 acgaaaaaga ctatgccact cctgaatcgg cccacgtaag gcatattctc gtggagacgg
     6301 aagcagaagc gaccaatgtt cttctcgagg tgtacggcag ggatgccgat tttggtaccg
     6361 tcgccatgaa gtacagcaag gatcccggca gtgctcagaa aggcggggac ctgggctggg
     6421 ttttcagggg acagacggtg ggagagttcg acgagatggc tttctccctg gagccgggca
     6481 atacaggtgg tccggtggag acgaagttcg ggtggcacat cattcaggtt cttgatcgcg
     6541 cgccagccag agcgccggtt tttgacgagg ccatcaacca ggtcagggag gacttgcagc
     6601 agcactatct tgccatggag gtcgagcgtc tcggcggttc cctgggtgtc gaggtcgaca
     6661 gggatattct ctccactctc ggtggttttc ccgcatacag cgcgaccgag tagaaagttt
     6721 gtttgcgaag acggggcggc cgaaagcagg tcgccccgtc tttttatatc tggagttctc
     6781 ctctcaggac cacgcaggcc gggcctgata ttctgaccct ttcaccagag agagcgagat
     6841 gcagttcacc gccgcggcga gaggcctgga aggcgctgaa atcttttttt ccagtgacgc
     6901 ctgaccagta tggagccagt acggtgtgag ccgatcctgt tacggggtcc tcgttcaggc
     6961 cttcccacgg accgaaatac cttgatacaa agtcgtaccg tcctgagggg gacgtcacta
     7021 ttaccccctc atacttctcg gggcccctgt cggctcgaag catcgcctcg aaatccgggg
     7081 acaggttccg gacggtgtcc tcatcgccaa ccctgacgag aagcatcagc gcttcctttg
     7141 cgatgaggac ctcttccggc cccgttattc ccattgctgc catgacctct gccggctgct
     7201 ctgccggaac cggcctgtcc gccggaaaat caagggtgat tcccttcatc gagagttcgg
     7261 caaaaagggt gccagaggcg ctctcgaagg agatccttcc gccgtttttt tcctgttcgc
     7321 tgaagatgac gtgggaggcc gcgagggtag catgtccgca gagagggacc tccacggtcg
     7381 gagtgaacca cctgatgctg aacgaatcgg gatcatcctt ccatccactg tcccgtggga
     7441 tcacgaaagc cgtctcggag agattcatct ccatggcgat gtttttcatg gttgcctctg
     7501 aagggaattc ttccagcagg cacaccgcgg cggggtttcc cccgaatggg gagggactga
     7561 aggcgtcgac ctggaaaaga ggaatcttca ttcctttcga tccattctgc atttgtctgc
     7621 cccctttcat gttttccacg agtatagccc acgatgatgg cgtctgtccc ccggtttggg
     7681 atattatcct tggaagggga ggggacggca tgattctgga aagagcgcgc ttcgatgtgg
     7741 caaggtacgg caaaataatg tccgaaaagg ggctcgtcac ggggacagga ggcaatttga
     7801 gcattaggga aggggacctt gtagcgatct cgccgagcgc ggtggattac tccctgatca
     7861 tgcccgaaga tgttgtggtg acggacatga agggcgtggt ggtcgagggc aggctgaaac
     7921 cgtcaagcga gatccctctg caccttgccg tttacggctc caggcaggat gtcctgtcgg
     7981 tggtgcatac ccactcacct aaggcatgtg tcatggcctg cctcgggtgg gagatacctg
     8041 tttttcacta tctcgtggcc cttgcgggga aaaaggtccc cgttgctccc tatgcccttt
     8101 tcgggacaga tggactggcc cgtaacgtcg tcgattccct gggagaagag aaagctgtcc
     8161 tgatggccaa ccacgggctg ctagctgtcg gaaaagatct tcccgaagca ttcaggctgg
     8221 ccgaaacagt ggaagtcgtc gccgggatat atgcgtcagt aaagtccctg ggagaaccaa
     8281 aactgctcac ggaagcagaa acggaagccc tggccagagc ttttgccgaa tatgattccg
     8341 tgagcagggg aaacaaaccg ggccgtatct agtttccgaa catggattcc aggctttccc
     8401 tgatggatcc catgaaagag tccgtttcct ttggcggagt cgtagcaagg ctcaccataa
     8461 cgaataccgt cccggagaga agaagccccc acaggggcgg ccagtggccg aggggtttga
     8521 gtccctgaat atacatgtac cctactgtca gccctccggc tatcatgctg gagagtgcgc
     8581 cggccgccgt agccctcttc cagaagaaga cccccagtat tgccggcaat tgcatcagca
     8641 gtcctcctga agccattgac gaaagtacgg cgatcagccc cagtttcatt ttcgcgaaga
     8701 aaaagcatgc cactgtaatg accgggatga gggtccggca tatcgcaagt tccgattttt
     8761 ccgtgatacc tggggccaat gccttcacga catcccttcc gaacatggaa ctcagggtca
     8821 aaataaccga gttcatggtt gaaacagctg cacccatgat gctcagcgtc acgagaagcg
     8881 cgatcgggac cgggaccgag cccaggagca tggccatggc attgtccggt ttgtccagcc
     8941 ccgggaaacg tgccgccgcg gccagtccga aaaccacgca gagaacggtg tatatgaacc
     9001 cgaaggcaga aaagcccaga accatttttc gcaggttggc cgcgctggcc ggagcgtaaa
     9061 gccgctggac cacctgcggg ttggttacgg cgaagaaggc ccatggaagg gtcaacccga
     9121 cgaacagggg gaaggaccat ttcagtttca ggaggtcgga gttcatcgca agggcgttgg
     9181 agaaaccttc cgggaaaagg cggaaggcta tgaaatagag aaggatcagg ctcgcgaaaa
     9241 gcatgataac ggcctggagg gcatctgtga tcgccacgga gcgcaatccg gcccaccagg
     9301 aaaagaccag ggacacagcg gcggctatca gcatgccggt aatgaaaggt atggtcccgc
     9361 cggaaagagt ctcaaagaga tagcctatcc ccatcaattg aaccgaagca taaggtataa
     9421 gcatgacgag acacagaatc gccgagaccg cccccgccgc cggactttcg tatcttttgg
     9481 aaagcagttc agaaggagtc accagattga atattcgtcc tgccgcccag tatcttgggg
     9541 caaagagcac catgagaatg actgtcatgg cgagataggt catttcgaaa ccgagggctc
     9601 ctatcccgga tgaataagtg agaccgacga gcccgaccat catgaaagcg ctgtaggtcg
     9661 tcgcgctgta ggtcatggcc gagacgaatc cccccagttt acggtttgcc agaaaatact
     9721 cggacatccc ctggcccagt ttctttcggg catgccagga aagaagagac ccggcaacga
     9781 accagacggc ggtacctgcc agaataagag aggcgttcat ttcgatgccc cgcttcgtgg
     9841 ggtgccgctc caggatgatg tgacccatgc tcccgcaagc agaaccgcca gtgttatgac
     9901 gttccagaac atgaaagcgc ccccgaggga tgccacctcg cggagaaagg tgaagggaat
     9961 ggctatattt gctgttccat agaccaatgc gaaaattacc cagatctttt cacggctgtt
    10021 cattttttga cctccccggg ctatgcttca agaaagccct tttctgaaag tgcgttctcg
    10081 actgcgtcga gggtaatttc gtccggagcc tccaccgtgt ggagatggaa tccgtttgaa
    10141 agagagagaa ggggagactg gcccgaactt ttgaaaaggt ttacgaaacg gatgacgtct
    10201 tcacgggttg ccagatcgag gttcccccgg atctctccgt ataccggatg gtcgactacc
    10261 acgtccagga cccttcctcc cgcctcgatt atcgtgttaa gttcttccgg gatctcgtct
    10321 gcgccgtgcc tcacggcgaa gactctcctc cggctgtcgc ctctcaggac ataaccccgg
    10381 cttgtggcca tgatccgggc tccccggtct ctgaggagtc cgatatcctg tacaaccgcc
    10441 tggcggctta tgtcgacttc ttcggccaga tgtatcccgc ttacgggttc ttcggattct
    10501 tccagaatgc ggacgatctg cctgagccga ttttcccttg aaagcaaagg ctcacctcct
    10561 caattaatgc aagtagcagc cactagcgga taatagcata gagccgcagg ctatggcaag
    10621 ctgattccgg actgggcctt gaggagcaag tgggttaaca tgtgtgaatg agggatttgc
    10681 agaaaggtga tgccccggcc ttcaggtgga gcagggttgc cgttcatgaa gccgggcatg
    10741 ccgtaatggc gatgcttctc gggctggatg tcgtcgagtg cgatattacc aggggaaggc
    10801 ggggccgcgg aagatccgga aggtgtgtag ttgtcgatcc ggatcccgtg ggattaaggc
    10861 gcttccttgt ctccatggga ggtatactgg ccgagcacct ggtctttgga gacgaccgcg
    10921 gtgggggaaa agacagggag gatgcagggg tctctctttt tgtctactcg tcgaagtaca
    10981 ggcagaaggg atcggatcct ggagcggagg caattattct tgaggtctct gaactgttcc
    11041 gggaacaggc aagcagaacc gtcctgatgg atatagcttc cctacttgac cgtgacggaa
    11101 gagttgatgg cggcaggctg ctggtttccg gcaacagaat cggggaatct ctggatatct
    11161 ccgggatcga aaaaaagatc gcggattttg ccgaggctgt ccctgctcca gggtggaagg
    11221 atatgttact gggttggata tcgaacataa aagaaatcat tgaaagaatc cgtggcttct
    11281 agatgctgct ggcaggtcag actacagggc agggagatga agaccgaatg agaagcatga
    11341 tcgaaatgga aaagatgaac ccctttgaag cgagggagaa gatacggctg ggggaatgga
    11401 caggacccac ggcgggaatc tcaggcgggt ttgtccaggc caatcttgtg gttcttccca
    11461 gggactgggc ttttgatttt ctcctttttt gccagaggaa cccgaaacca tgtcctcttc
    11521 tggatgtgac cgagcccggt gaccctgtac cgcgccttct cgctcccgga tccgatatca
    11581 ggaccgatct gccgaaatac agggttttca gggacgggat cctggatgac gaaccgctgg
    11641 atgtcaaatc gatatggagg gacgacctgg tgggttttct catcgggtgc tcctttacct
    11701 tcgagggagc cctgatggag gccggtgttc cggtacgaca tgtcgaggaa ggggtcaacg
    11761 ttcctatgtt cataacctcc atcccgtgcc gtccagcggg aaggctctcg gggccaatgg
    11821 tggtgagcat gcgccccgtc cccccggaaa tggtcgcaag ggcggtcacc gttacggcaa
    11881 tgtatcccgc tgttcatggt gccccgatac atgtcgggtc tccggggtcc atagggataa
    11941 ccaatatctc gcggccggat ttcggtgacc ctgtaaccgt gagggaaggt gagacacctc
    12001 ttttctgggg ttgcggagta accccgcagg ctgcactgat ggcctccagg cccccctttg
    12061 ccataactca ttcgccgggg cacatgttta tatccgacag aagggacagc gaatacgcgg
    12121 ttttctaaaa aaactgtcac gcccccgctt ttgcctgtcc cgtttaccgt tgaccgtttc
    12181 ccgtttacgc agttgaattt aaggatcttg ccttccctgc ctcccgtatc ctgtatccag
    12241 acgccttgct tttgcctgtc atcctgagcg cagcgaagcg gaacgtccgc ctaccggcgg
    12301 agatctgacc ccaagacctt ctcaccacag agatacgcag agggtggggc cccgcagaga
    12361 aaaccaattt ttgggagaac cggggaccca agatttgtca ttctgaaccc gaagggtgaa
    12421 gaatctggtt ttggtgtttg atctttcgac tcacgattcg cggcactcaa atcagattcc
    12481 cgccggtagg caaagggcgc ggaacgtccg cctgctggcg ggttccgctt cgctccgctc
    12541 agaatgacaa gcctcttgcc cttcctgttt cctgcctcca gtctcccgac tcctgccgcc
    12601 tcgccttttc gtctcacctc t
//
LOCUS       DAOTIQ010000054        12590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_5971_length_12590_cov_6.960909, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000054 DAOTIQ010000000
VERSION     DAOTIQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5971_length_12590_cov_6.960909"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="P5541_07225"
     CDS             complement(<1..566)
                     /locus_tag="P5541_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRV71813.1"
                     /translation="MSALWKAAGDFLLFVLNSLYSFTHSYGIAIIVLTILIRIVLHPL
                     QHKQLVSMQKMQKLQPRMKILQEKYAGDKETLNKEIMKLYKEHNVNPAAGCLPLLVQL
                     PILILLFRVLMNLDLGGAGFLGISLEGSVLSTLGSAVGLPADVTGIGAVLSGIMGNPS
                     GLLRLGVYAGNFVLLVLVGFLTWLQQKL"
     gene            complement(572..856)
                     /gene="yidD"
                     /locus_tag="P5541_07230"
     CDS             complement(572..856)
                     /gene="yidD"
                     /locus_tag="P5541_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRV71814.1"
                     /translation="MTRLPSRIAICLIRFYQVFISPLLGDNCRFYPSCSSYAMESISR
                     FGFSRGLVLSFLRIIKCGPWNSGGFDPVPDEFPRFFSGFSENKEYSPRGR"
     gene            complement(853..1254)
                     /locus_tag="P5541_07235"
     CDS             complement(853..1254)
                     /locus_tag="P5541_07235"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRV71815.1"
                     /translation="MRIRFGYPRQYRLRKGWQFDLVFRTGRRVNGALVRLLFLPSPDG
                     TTAFGVAAGKHQGSSPVRSRSKRVLRESIRRLRPWVREGFWVICMLRNSALGRNARDV
                     YEEMARLFRRSGLMEKDWPGVDWMSDRSVSE"
     gene            complement(1255..1389)
                     /gene="rpmH"
                     /locus_tag="P5541_07240"
     CDS             complement(1255..1389)
                     /gene="rpmH"
                     /locus_tag="P5541_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRV71816.1"
                     /translation="MKQTFQPHNRRRKRRLGFLQRSSSPGGRRILRNRRSKGRKRLAV
                     "
     gene            complement(1504..3453)
                     /locus_tag="P5541_07245"
     CDS             complement(1504..3453)
                     /locus_tag="P5541_07245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRV71817.1"
                     /translation="MSRTGTGLITPSMEDIFSEKGLLSRNLGPFEYRPQQEDLARSVW
                     EALESGKGDILLAEAPTGTGKTHALLVPAVYWSLRNDTRVLFLTSGIPLQEQIVRRDL
                     PELCRCLGIKEFPFGLIKGKSNYLCRLRGKELSSRELMPLLEPEAAAELEAWMESTET
                     GDFSEISLPPSHPVLSAVAAGSDICRGNACPFRDTCFLKKVFQQASEWRITVANYHLF
                     FSYAASVDSGFPVPFDAVICDEAHRLTEAARTAATKRSSAEDWLRLLDYGRARVLPAL
                     DWLSTEKISDAISAAERVKLEAETLFSGWKKKMSHGEVFGTGARPPEGAEELVGSTEE
                     MLRIVGKPPDSSEKDKNREKHAVELAQWREGMLEILDSFRWCSAVGRFPEWAYWWDGR
                     SIMSAPSDCSGFIREWFFPKPLHALVAVSATMAIGNDFSFWESETGLRGTEKMVLDSP
                     FPLEEQMKVWIVDMGLRVTDEAYDRNVAAVVEKLCEENGGSTLVLLSSMRLMRSVAEK
                     LGKTPRPWPVLVQGELPRNELLRRFREEISSVLVGSVSFREGIDVPGDGLTQVIIDRI
                     PFPHPGDPLLVTRRAMEGPAIFTEMILPLAKLLLKQAAGRLIRSRIDRGKVVILDGRV
                     LGRRDWRITEALPKVVYRKVKVAVR"
     gene            complement(3450..4097)
                     /locus_tag="P5541_07250"
     CDS             complement(3450..4097)
                     /locus_tag="P5541_07250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRV71818.1"
                     /translation="MNAGLLTAVAAAPGLALLWWFYNRDIIEPEPLSLVFSAYLRGML
                     LVIPAGLLEMLVEDFLVFSPFLSVFAGVALVEEYFKWIALRRYIVSPECDECYDGIVY
                     GTSVALGFATLENIFYVVGSINPWAVAGWRALLSVPLHALCGLAMGYEAARQKMSGYE
                     KFSLWRILWFPVAAHGFYNYLLLTGTVGGVLAAMLLVAVMWITGFRLMRRSRNCP"
     gene            4225..4497
                     /gene="rpsT"
                     /locus_tag="P5541_07255"
     CDS             4225..4497
                     /gene="rpsT"
                     /locus_tag="P5541_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRV71819.1"
                     /translation="MPNKKSAEKRVRTSERNRIYNRFWTTRCKNSARKVLEAVETKDM
                     EMAVKRLDEAQSALDKAVVKGVIHRNTAARRKSRLTRAVKSLAPQA"
     gene            complement(4522..5493)
                     /locus_tag="P5541_07260"
     CDS             complement(4522..5493)
                     /locus_tag="P5541_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71820.1"
                     /translation="MPQFIAVAGTGPAQRRLLSDCTAELKKGGCGSVERFSGDSWQDL
                     LDISRTGGLFEENRAVIVESADALGPFPEEFFGEIEGSAACVTFLLVFDGDPSKMLPT
                     RQRKEITVRKPAAVPYFFGERVRWVEREARSRKIYINKGAINLLVEFVEDPEVILSEL
                     IKLAAAAGEKLIDEDLASSLIMDDGGRQMLKLLDGICRGETSAVIDALSILRRREVPI
                     KIISALHKRMRMAMYLSLAGEKWREAAETALEITKYQAGLAKKSIDLFPRNALRDFVL
                     AMARESALFKTGLGSGWDGIELEIIRLLASAGKKERHGENSVAAIAG"
     gene            complement(5493..7979)
                     /gene="leuS"
                     /locus_tag="P5541_07265"
     CDS             complement(5493..7979)
                     /gene="leuS"
                     /locus_tag="P5541_07265"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRV71821.1"
                     /translation="MGYDFVSIEKKWQKRWEKEGTFNVEKDSSREKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMLSRHLLMRGKNVLHPMGYDAFGLPAENAAIKFSTHPNKWTWDN
                     IEHMTDQLKRMGCSYDWRRRVETCNPNYYRWTQWLFLQFFKRGLAYRKTAPVNWCESC
                     GTVLANEQVINDGHCWRCSTPVVKKNLEQWFLRITDYAQELLDSLDDLPGWPERVKLM
                     QRNWIGRSEGVRLSFKLEGLQTDIETFTTRVDTIFGVTFLALSPEHPAVEEIAALSPE
                     GARIRKFAAECMDSSERDRTAAGGEKKGIDTGLVAVNPANGEKVPVWIANYILMEYGT
                     GAIMGVPAHDQRDFDFARKFEIPVRPVITPPGQNILDGESMTVAYEDDGIQCNSGKFD
                     GLSTSEALPAMTEWFAENGWGKREINFRLRDWLISRQRYWGAPIPVIYCDKCGVVPVP
                     EEDLPVELPLEIEMKPGENPLAGADNWKKARCPRCGGEGTRETDTMDTFICSSWYFLR
                     FASPWTEDAPFEKDDTDYWMSVDQYIGGIEHACLHLIYARFFTKFLADLGMLKVREPF
                     RNLLTQGMVIKDGAKMSKSKGNVVDPDEIISRWGADTARLFILFASPPEKDLDWSDQG
                     VEGSHRFLQRVWRLVETNLEGLKKTSSDTVPMSAITENRCRDLKRRIHYTIDRVTRDI
                     QDEKQFNTAVARLMELSNTLGAFSPKSPAEWSLFREGVDTLLVCLTPIAPHICEELWE
                     ITGKKTLISLEEWPVIESEALVADTVTIVFQVNGKVREQFELPAGMDPEEMKKEILSR
                     PEVKSRLEGKEILRVIPVPDRLVNVVVKNR"
     gene            complement(8150..8932)
                     /locus_tag="P5541_07270"
     CDS             complement(8150..8932)
                     /locus_tag="P5541_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRV71822.1"
                     /translation="MLRGLFEIIFSMGSYVGALVIIVVLLAAALKIIPEYQRGVVFRL
                     GRFVGSKGPGLVIVIPVIDKLFKVDLRVVTLDVPYQEVITKDNVPVKVNAVVYFRVID
                     PAKSVIEVENYIMATSQLSQTTLRSVVGRAELDDVLSARDKINLELQQIIDERTDPWG
                     IKVSAVEVKELELPEGMKRAMARQAEAERERRAKIIAAEGELQAAEKLTQAAVKMEES
                     AITIQLRYLQTLREVASENNSTTLFPIPIDMMKAFFEGKGKK"
     gene            complement(8967..10313)
                     /locus_tag="P5541_07275"
     CDS             complement(8967..10313)
                     /locus_tag="P5541_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRV71823.1"
                     /translation="MKKHLLLPMLISIIVLSGVTGLPLSASGDQEGGAVIVSAKIEGV
                     VGIALESHLEEVMEVATEKEAAMLVLVMDTPGGLSSSMKGVIQQIEKAPFPVVVWVSP
                     SGSRAASAGAFIVQAAHIAAMAPGTNMGAAHPVVASGGDIPDEDMKTKVLSDFAAQIR
                     ALAEQRGRNPEAAEAMVTESVSYTSTEALEAGVIDLVAADLDEIVAWSKGKKFIIGGA
                     ETVVDTSGYRVVEIEMSPRLRALQFISRPDVAYLLLVAGVFAIVFEVLSPGGFVLGVS
                     GGMLVLFGAYGLRMIPFNWAGIVFLLAGVAVMILDLAVGGIGILSLLGAGAVIVGGLI
                     LFRAPGGELLNMSYSLMTGAVVIITGFFILAAWAVWKSLGNRPVSGSEGLAGAEVLLI
                     TDLSPAGSVMCRGEIWKARSADNSNIESGSAGVVERVDGLTLVVKMTKAGGSGEER"
     gene            complement(10310..11647)
                     /gene="radA"
                     /locus_tag="P5541_07280"
     CDS             complement(10310..11647)
                     /gene="radA"
                     /locus_tag="P5541_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRV71824.1"
                     /translation="MARRQQRFVCAECGHISSTWAGRCPSCQEWGSMVEDRPVVSRGK
                     ESGNSVIPRGIGEITIPERLSSGMGELDRVLGGGFVPGSSVLLGGEPGIGKSTLLLQA
                     CTAMAAKGMKVLYVSGEESLSQVAARAGRVGGRPGDGLGIISSANLDESLGSMDGHSI
                     AVFDSVQSFTTEEGGSFPGTTTQVRAAAQKIMERCKAMDVPALIVGHITKQGSIAGPK
                     LLEHMVDVVLLFAGDRSSPYRLLRSAKNRFGSTDELGVFEMTGKGLVPVQDPGRLFWS
                     TAKEPVSGVAMGVILEGSRPFVAEIQALVAPTPFPYPKRTARGVDAGRLQLMLAVLDR
                     RCGISSGSLDVFVNVAGGMTVKDPYSDLALCMAVGSASLDAPLPPDCCFIGEVGLAGE
                     VRPALRTPLRLREAGRLGFRKGVISRYETDEMPEGIDVIRVGNLKEALRVVKK"
     gene            11785..>12590
                     /locus_tag="P5541_07285"
     CDS             11785..>12590
                     /locus_tag="P5541_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV71825.1"
                     /translation="MYHWLIMSLFAASSIFVTLVFILVGIVSARGSGATDYSMAGRKS
                     TVTGVSGILLGSLVGGASTVGTVQMAYQWGISAWWFTLGGGIGCLVLGIWFAVPLRRS
                     GMETIPGFLSLSYGPKAGLVAVTASASGTFLSIVAQFLSGAGLLGGVVSAGPLVSFSL
                     VAILVVSFIFLGGLRSFSSIGAGKIILLYFTLSICAAKAVLLLVSFDGTPVSMPVQPF
                     FSLFGQGIGNGLGSGASLVTGVLCTQIYIQAVFSASSPSTARKGAILSAIL"
BASE COUNT         2763 a         3801 c         3055 g         2971 t
ORIGIN      
        1 ctgagtttct gctgcagcca ggtcagaaag ccgaccagca ctagaagaac aaaattgccc
       61 gcatagaccc caagtctgag aagaccggac ggattcccca ttattccgga aagcacagca
      121 ccgataccgg tcacatctgc gggaagccct accgcgctgc caagtgtcga aaggactgaa
      181 ccttccaggc tgatacccag aaatcccgcg cctccaagat cgaggttcat aagaacacgg
      241 aaaaggagaa tgagtatagg aagctgaacc agcagcggga gacaccccgc ggcagggttg
      301 acgttatgct ccttgtagag cttcattatc tccttgttga gggtttcctt gtcgcctgcg
      361 tacttctcct ggagaatctt catccggggc tggagtttct gcatcttctg catgctgacc
      421 aactgcttgt gctgaagcgg gtgaagcacg atcctgatga gaatggtaag gacaatgatc
      481 gctatgccgt acgaatgtgt aaaagaatac agactgttaa gaacaaaaag caggaaatct
      541 cccgccgctt tccaaagtgc actcaaagga ttcaccttcc tctgggagaa tattctttgt
      601 tttcggaaaa accggaaaaa aaccgcggaa actcatccgg gacagggtca aatccaccgc
      661 tgttccaggg accgcatttt attatacgca aaaaggagag gaccaaaccc cttgaaaacc
      721 cgaatcgcga gatgctctcc atggcatagc tggagcacga ggggtaaaac cggcagttat
      781 cacccagcag gggcgatata aagacctgat agaacctgat caggcagatg gcgatccttg
      841 atggaaggcg agtcattctg acacactcct gtccgacatc cagtccacgc caggccagtc
      901 cttctccatc aggccggatc ttcgaaaaag tcttgccatc tcctcgtaga catccctcgc
      961 gttccttcca agggcagagt ttcttagcat gcatatgacc cagaaacctt ccctgaccca
     1021 ggggcgaagc ctccttatgg attccctcag aacacgcttg cttctggacc tcacagggga
     1081 agaaccctgg tgttttcctg ccgccacacc aaatgccgtg gtcccatcgg gggaaggcaa
     1141 aaacagcaac cgcaccaacg cgccattcac ccggcggccg gtgcggaaaa caaggtcaaa
     1201 ctgccagccc tttcgaagcc tgtactggcg cggatagccg aatcgaatcc gcaactagac
     1261 cgccagccgc ttcctgccct tgcttctcct gttcctcagt atacgcctgc cgcccggaga
     1321 actggagcgc tgaaggaaac caagccttct ctttcttctt ctattgtgtg gctggaaggt
     1381 ctgtttcaag ttcgacacct cctcctgcaa ttgtcataca ccataatacc accggaaaaa
     1441 aaccagcccg gatactatag cataccggga aaccggcaac aagtacgaaa ctccagattc
     1501 acgctacctg accgccacct tcactttcct gtaaaccacc ttcgggagcg cctcggtgat
     1561 cctccagtcc cgcctgccga gaacccggcc atctagaatg accacttttc ccctgtcgat
     1621 ccggctccgg ataagcctgc ccgcagcctg cttgagaagc agttttgcca ggggaaggat
     1681 catttcggtg aagatcgctg gaccctccat tgcccttcgt gttacaagga gagggtcgcc
     1741 aggatgcggg aagggtatcc tgtcaattat gacctgggtc agaccatcac ccggaacatc
     1801 tataccctca cggaaagaga cgctccccac cagcaccgat gatatttcct ccctgaaacg
     1861 cctgagcagt tcattcctcg gaagttcccc ctggacaagc accggccatg gtctgggggt
     1921 tttccccagt ttctccgcga cagatctcat cagcctcatg gaactcaaca ataccagcgt
     1981 actgcctccg ttctcctcgc acagtttttc cactactgct gcaacatttc tgtcgtaggc
     2041 ttcatcggtt accctgaggc ccatatccac tatccagacc ttcatctgct cttccagcgg
     2101 aaaaggggag tcgagaacca ttttttctgt gccacgtagc ccggtctcgc tctcccagaa
     2161 agaaaagtcg ttccctattg ccatggtcgc cgatacggca acgagcgcat gaagaggttt
     2221 gggaaaaaac cactccctta tgaacccaga gcaatcggaa ggagcactca ttatcgacct
     2281 gccatcccac cagtaggccc attcagggaa ccttcccact gcggagcacc agcgaaagga
     2341 atccaatatc tccaacattc cttccctcca ctgggcaagt tcaactgcat gtttttccct
     2401 gtttttatcc ttctccgaag aatccggagg tttccccacg atcctgagca tttcttcggt
     2461 acttccgaca agttcttcgg ccccttccgg gggtcgcgct cccgtcccaa atacctctcc
     2521 gtgactcatt tttttcttcc atccggaaaa aagggtttcg gcctcgagtt tcactctttc
     2581 agctgccgag atcgcatcgg atatcttctc tgtggaaagc cagtcaaggg ccggtaatac
     2641 cctggctctt ccgtaatcaa gcaatcgcaa ccagtcctct gcggaacttc tcttcgtagc
     2701 ggcggttctt gcagcctcgg tcagcctgtg agcctcatca caaataacag catcgaaggg
     2761 taccgggaac ccgctgtcca ctgaagcggc gtaggaaaag aaaaggtgat agttggctac
     2821 agttattctc cattcagagg cctgctgaaa cacttttttc agaaaacagg tgtctcgaaa
     2881 ggggcatgcg ttccccctgc atatatcgct tccggccgct acggccgata gcacgggatg
     2941 ggatggcggc aacgatattt cggagaaatc ccccgtctcg gtactttcca tccaggcctc
     3001 gagttcagcc gccgcctctg gctcaagcag tggcattaat tccctggacg aaagttcctt
     3061 cccccggagc ctgcacaggt agttgctctt tcccttgatg agaccgaagg ggaattcctt
     3121 tataccgagg cagcggcaaa gctctggaag gtctctcctg actatctgtt cctggagagg
     3181 tatccccgac gtgagaaaaa gcaccctggt atcgtttctg agagaccagt agacggccgg
     3241 gacaaggagg gcatgggtct ttcctgtccc ggtaggagcc tctgcaagaa ggatgtcacc
     3301 tttgccagac tccagcgcct cccagacgga cctggcgaga tcctcctgct ggggtctgta
     3361 ctcgaagggt cccaggtttc ttgaaagaag ccccttctca gaaaagatgt cttccatgga
     3421 tggcgttatc aagccagtac cggttctgct catggacagt ttcgcgacct cctcatgagc
     3481 ctgaagcccg taatccacat aactgccacc agaagcatgg cggcaagcac tcccccaact
     3541 gtcccggtca gaagaagata gttatagaac ccgtgagcag ccacgggaaa ccagagtatc
     3601 cgccataagg agaacttttc ataacctgac atcttctgtc ttgcggcctc gtagcccatg
     3661 gcaagcccgc aaagggcgtg aaggggaaca gaaagaagcg ccctccaccc cgccaccgcc
     3721 caggggttga tcgagccgac aacatagaag atattctcca gagttgcaaa acccagagca
     3781 acgctggtcc cgtaaactat cccgtcataa cattcatcgc actccggcga gacaatgtac
     3841 ctgcgcaagg cgatccattt gaagtattcc tcaaccagcg ctaccccggc aaagactgaa
     3901 agaaagggag aaaaaacaag aaaatcctcg acgagcattt ccagaaggcc agcgggaata
     3961 accagcaaca tcccccttaa ataggcggag aaaaccagtg aaaggggttc cggctctatg
     4021 atgtccctgt tgtagaacca ccagaggagc gccaggccag gcgcggccgc gacggctgtc
     4081 aatagtccgg cgttcatttt ttttgcgcct ccttctggag attcgtgata gaataatccg
     4141 gtttcaccga taatattctc cgcttgttgc ggataacgca agcgccatga taaaatgtgg
     4201 cctgtttgca aagggggtga catgatgccg aacaagaaat ctgccgagaa gagagtccgt
     4261 acttccgaaa ggaacaggat ctacaaccgg ttctggacga ctcgctgcaa gaacagcgcc
     4321 aggaaggtac tggaggccgt tgaaaccaaa gacatggaga tggccgttaa aaggctggat
     4381 gaggcgcagt ccgctctgga caaggctgtg gtcaaggggg ttattcaccg caacactgca
     4441 gcgcgccgga aatcaagact gaccagagcc gtcaagtctc tcgcacccca ggcctgatgc
     4501 gccgcaaacc cctttatatg atcatccggc aatcgccgcc acggagtttt ctccgtggcg
     4561 ttcttttttc ccggcactcg ccagaagcct tattatctcc agttctatgc catcccatcc
     4621 gcttccgaga ccagtcttga agagagcgct ttcccttgcc atggcaagga caaaatcacg
     4681 gagggcattg cggggaaaca ggtctatgga ttttttcgca agtcctgcct gatatttcgt
     4741 tatctccagg gcagtctctg ctgcctcccg ccatttttcc ccggccaggg aaaggtacat
     4801 ggccatacgc atcctcttgt ggagggcact gatgatcttg atcggtactt cccgtcgcct
     4861 gaggatcgag agagcatcta tgaccgcaga tgtttctcca cggcagatcc cgtccagaag
     4921 tttcagcatc tgccgtcctc cgtcatccat gatcagcgaa ctcgcaagat cttcgtcaat
     4981 gagtttttct cccgcggctg ccgcaagttt tatcagttcc gaaagtataa cctctgggtc
     5041 ctccacaaac tccaccagga gattgatcgc ccctttgttt atgtatatct tcctggacct
     5101 ggcctcccgt tcgacccacc tgacgcgctc gccaaaaaaa tagggaaccg ccgccggctt
     5161 cctgacggtt atttcctttc tctgccgtgt gggcagcatc tttgaaggat cgccatcaaa
     5221 taccagcaga aaggttacac aggcggcgga tccctctatc tccccaaaga actcttccgg
     5281 gaagggaccg agggcatccg ccgactcgac tatgacggca cgattttctt cgaaaagccc
     5341 cccggtcctg gaaatatcaa gaaggtcctg ccaggaatcc ccggagaagc gctcaacaga
     5401 gccgcatccc ccttttttga gttccgctgt acaatcagaa agaagccttc tctgtgccgg
     5461 gcccgtcccg gcgacggcta tgaattgagg cactacctgt ttttcaccac cacgtttaca
     5521 agcctgtccg ggacggggat cacacgcagg atctccttgc cttcaagcct gcttttgacc
     5581 tccggccggg aaagaatctc ttttttcatt tcttcgggat ccatccctgc cgggagttcg
     5641 aactgctccc tgacttttcc gttgacctgg aaaactatcg taaccgtatc ggctaccagc
     5701 gcttcacttt cgataaccgg ccattcctcc agggatatga gagtcttctt tccggttatt
     5761 tcccagagct cctcacagat gtgcggggct atcggcgtga ggcaaaccag aagcgtatcg
     5821 actccctccc tgaacaacga ccattctgcg ggggactttg gagagaatgc cccgagggta
     5881 tttgaaagtt ccatcaacct tgctacggct gtgttgaact gtttctcgtc ctgaatatcc
     5941 ctggttaccc tgtctatggt gtagtgtatc ctgcgcttga gatccctgca cctgttttcg
     6001 gtgatggccg acatgggcac ggtatcggat gaggtttttt tcagcccttc caggtttgtc
     6061 tcgacaagtc tccagaccct ctggagaaac ctgtgggatc cctctacccc ctgatctgac
     6121 cagtccaggt ctttttcggg cggcgaagca aaaagaatga acagcctcgc tgtatcggct
     6181 ccccacctgc tgattatttc gtcggggtcc accacattgc cctttgattt ggacatcttt
     6241 gccccgtcct tgatgaccat cccctgggtg aggaggtttc ggaagggttc ccttaccttg
     6301 agcattccca gatcggcaag gaattttgtg aagaaccttg cgtatatcag gtgcaggcag
     6361 gcgtgctcta ttccccctat atactggtcc acggacatcc agtaatcggt gtcatctttt
     6421 tcaaaggggg catcctctgt ccatggagag gcaaatcgca ggaaatacca tgatgagcat
     6481 atgaaggtat ccatcgtgtc ggtctccctg gtcccctcgc ccccgcacct gggacacctg
     6541 gctttcttcc agttatccgc cccggccaga ggattctccc ccggcttcat ttctatctcc
     6601 aggggaagtt ccacgggcag gtcttcttcc ggcaccggga caaccccgca tttgtcacaa
     6661 tatataacgg gtatgggagc accccagtat cgctgcctgg atatcagcca gtcgcggagc
     6721 cggaagttga tctccctttt gccccatcca ttttcggcga accattctgt catcgccgga
     6781 agggcctcgc tagtggaaag tccgtcgaac ttgccggaat tgcactgtat gccatcatct
     6841 tcataggcaa cggtcatgct ttctccatcg aggatatttt gtcccggggg agtgatgaca
     6901 ggccttaccg gtatttcgaa ctttctcgcg aagtcgaagt ctctctggtc gtgggcaggg
     6961 acacccatta tcgctccggt cccgtattcc atgaggatat agttggctat ccagacgggg
     7021 accttctcgc cgttggcggg gtttaccgcc acaagtcccg tgtctatacc ctttttctcg
     7081 cccccggcgg ccgtgcggtc cctttcgctg ctatccatac actcggcggc gaacttcctg
     7141 atccgggctc cctcgggaga aagagccgct atctcttcga cagcggggtg ttctggcgag
     7201 agggccagga aggtcacacc gaaaatagtg tcgactcggg tggtgaacgt ctctatatca
     7261 gtctgaaggc cctccagttt gaaggagagt ctcacccctt cggagcgccc tatccagttc
     7321 ctctgcatga gtttgaccct ctccggccag cctggcagat cgtcgaggga atcaagaagt
     7381 tcctgggcat agtcggtgat cctcaaaaac cactgctcca ggttcttttt tacgacgggg
     7441 gtgctgcatc tccagcaatg gccatcgttt atgacctgtt cgttggcgag gacggtcccg
     7501 caactctcgc accagttgac aggcgccgtc ttcctgtagg caaggcctct tttgaagaac
     7561 tgaaggaaca gccactgggt ccatcggtag tagttcgggt tgcatgtctc gacccttcgt
     7621 ctccagtcgt aactgcaccc cattctttta agttgatccg tcatgtgctc tatgttgtcc
     7681 catgtccact tgttgggatg agttgagaac ttgatggccg cgttctccgc ggggaggccg
     7741 aaggcatcgt aacccatggg gtgaagaaca tttttgcctc tcatcaggag atgccttgaa
     7801 agcatatccc ctatcgagta gttcctgaga tgccccatgt gaagggcccc gcttggatag
     7861 ggaaacatct ccaggcaata gaatttttcc cttgatgagt ctttctcaac gttgaaagta
     7921 ccttcctttt cccagcgttt ctgccatttt ttttcgatag acacaaaatc gtagcccatt
     7981 caggaatcct cctcgatgcg gagcactatg gcgcttccgg ttttcgcaat tggaacatat
     8041 tatatccgtc cggacctcaa gtcgcaaaaa aattgcggga gaagcgcgac agcctccccc
     8101 gcggtttcga tgcaaaaacc accccgtcgg ggcaactttc ccataacggc tattttttcc
     8161 ctttaccttc gaagaaggcc ttcatcatgt ctatgggaat cgggaaaagc gtggtcgagt
     8221 tgttttccga tgcgacctcg cgcagcgtct ggaggtatct caactggatc gttatcgctg
     8281 attcctccat cttgaccgca gcctgggtga gtttctcggc tgcctggagt tctccctcgg
     8341 ctgctattat cttggcccgg cgttcgcgct ccgcttccgc ctgccttgcc atggctcttt
     8401 tcataccctc ggggagttca agttccttga cttccacggc gctcaccttt attccccatg
     8461 ggtcggtcct ctcatcgatt atctgctgaa gttcgaggtt gatcttgtcc ctggccgaaa
     8521 ggacatcgtc cagctccgcc cttcccacga ccgacctgag ggtcgtctgg gacaactggc
     8581 ttgttgccat aatatagttt tctacctcga tgactgattt ggcgggatcg ataacccgga
     8641 aatagaccac cgcgtttacc ttgacaggaa cattatcctt cgttattacc tcctgatatg
     8701 ggacatcaag ggtaacgacc ctgagatcga ccttgaaaag tttgtcgatg accggtatga
     8761 ctatcaccag gccaggcccc ttcgaaccga cgaaccttcc aagtctgaaa acaacgcccc
     8821 tctggtactc cgggattatt ttcagagccg ccgcaagcag gaccactatt atcacaagtg
     8881 ccccgacata acttcccatg ctgaatataa tctcgaacag tcctctcaac attttctttc
     8941 ccccctttta ccgggatact gtcccgttat cgttcttcgc cggatccacc ggctttggtc
     9001 atttttacaa ccagggtaag gccatcgact ctttccacca caccggcaga acctgactcg
     9061 atgttgctgt tatctgccga gcgggctttc catatctccc ccctgcacat tacgctacca
     9121 gcgggagaga gatcagttat cagcaatact tccgcaccgg caagaccctc gcttccagaa
     9181 acaggccggt tcccgagcga tttccagacc gcccaggccg ccagtatgaa aaagcccgtt
     9241 attatgacaa cggcgcccgt catcagactg taagacatgt ttagcagctc ccctccaggg
     9301 gccctgaaga gtatgagacc cccgactatt acggcccctg ctccaaggag actgagtatt
     9361 cctatccccc ctactgcaag atcaaggatc atcaccgcaa cccctgccag cagaaaaact
     9421 attcccgccc agttgaaggg gatcatcctc agcccgtaag ctccgaaaag caccagcatg
     9481 cctccggaaa ctcccagcac aaaacctccg ggagagagaa cctcgaaaac tatcgcaaaa
     9541 acccccgcga caagaagaag gtaggctaca tccgggcggg atatgaactg cagcgccctc
     9601 agcctcgggc tcatctctat ctctactacc cgatatccgg aggtgtcgac gacggtctct
     9661 gcccctccga tgataaactt tttccccttc gaccaggcga ctatttcgtc cagatcggcc
     9721 gcgacaaggt cgataactcc cgcttcaagg gcctccgtgg aagtatagga tacactctcc
     9781 gtgaccattg cctccgccgc ctcggggttt ctgcctctct gctctgccaa agccctgatc
     9841 tgggcagcga aatcgctcag gacctttgtt ttcatgtcct cgtcggggat atcccctccc
     9901 gaggcaacta ccgggtgtgc cgcccccata ttggttccag gagccatcgc ggcgatgtgc
     9961 gccgcctgga caatgaaggc cccggccgaa gcggccctcg aacctgaggg agaaacccac
    10021 acgaccacag ggaagggcgc tttttctatc tgttgtatga cccccttcat ggaactcgag
    10081 agtcctcccg gcgtatccat aaccagaacg agcatggccg cctctttttc cgtcgcgacc
    10141 tccatgactt cctccaggtg cgactccagt gctattccaa ccacgccttc gatctttgct
    10201 gaaacgatca cggccccccc ttcctgatct cccgatgcgg acaagggaag accagtcact
    10261 ccggacaaca caatgatgct gatcaacatg ggtaacaaca ggtgtttttt cattttttca
    10321 ccaccctcaa tgcctctttc agattgccga cccggatcac atcgatcccc tccggcattt
    10381 cgtcggtctc gtatctgctt atgacccctt tcctgaaacc gagcctcccc gcctcccgaa
    10441 gccggagagg cgttctgagc gctggcctga cctcccccgc aagaccgacc tcacctatga
    10501 aacagcagtc cggtggaaga ggggcatcga gagaggccga accgactgcc atgcagagag
    10561 ccagatcgga gtaggggtcc tttacggtca tacctccggc gacattcacg aatacatcga
    10621 gagagcccga ggagataccg catctcctgt ccagtaccgc cagcataagc tggagccttc
    10681 ccgcgtccac ccccctggcc gtccttttag ggtaggggaa cggagtcgga gccaccaatg
    10741 cctggatctc cgccacaaag ggcctggaac cttcaaggat cacccccatg gccactcccg
    10801 acaccggttc tttggctgta ctccagaaaa gccttcccgg gtcctggacc gggaccaggc
    10861 ctttccccgt catctcgaat acacccagtt cgtcggtgct tccaaaacgg ttcttggcag
    10921 atctcagaag cctgtaaggg gaacttctgt ctccggcgaa gagcagcacc acgtccacca
    10981 tatgctcaag aagtttgggc ccggctattg aaccctgttt cgttatatgc cccactatca
    11041 gagccggaac atccatggcc ttgcatcttt ccatgatctt ctgcgccgca gccctgacct
    11101 gtgtcgtggt tcccgggaaa cttccaccct cctctgtagt gaatgactgc acgctgtcga
    11161 agacagctat cgagtgtcca tccatggacc ccagcgactc gtcaaggttc gccgaactga
    11221 tgatcccgag accgtccccc ggcctgcccc ctacccttcc agccctggcc gcaacctggg
    11281 atagcgattc ctctccggat acgtagagaa ccttcatccc ttttgctgcc attgcggtac
    11341 aggcctgcag cagaagagtt gatttgccta tgcccggctc tccgccaagg agcacggatg
    11401 accccggcac aaaacctccg ccaagaactc tgtcaagctc tcccataccg ctggaaagcc
    11461 tctccgggat ggttatctct cctatccctc tcgggatgac ggagtttccc gactccttcc
    11521 cgcgcgacac caccggacga tcctccacca tggaacccca ctcctggcag gagggacatc
    11581 tcccggccca ggtagaactt atatgtccgc attccgcgca tacgaacctc tgctgcctcc
    11641 tggccataag gtctctcctg tcatcgacct gtccgtgtcg atactgttat tatgattctt
    11701 tatcatagcg catcggtctt ttttttgcat ccttttcatt tcaaaaaaga gcagggttag
    11761 tcaaactttc atgactcttt tttcgtgtat cattggctga taatgtccct tttcgccgct
    11821 tcatccattt ttgtgaccct tgtctttatt ctggtcggga tagtctccgc tcgcgggtcg
    11881 ggagcgacag attattcaat ggccgggaga aaatccaccg tgacaggcgt ttcggggatc
    11941 cttctcggct cgctggtagg cggagcatca acggtaggaa ccgtacagat ggcctaccag
    12001 tggggaatat cggcatggtg gttcactctc gggggaggta tcggatgcct ggtgctgggc
    12061 atctggttcg ccgttccgct aagacggtcc ggaatggaaa ctatcccggg atttctgtcc
    12121 ctgtcttacg gccctaaagc ggggcttgtc gctgtaacgg catccgccag cggtactttc
    12181 ctgtccatag tagcccagtt cctttcggga gccgggcttc tcgggggagt ggtatctgcg
    12241 ggccccctgg tatccttttc attagtagcc atccttgtgg tgtctttcat cttcctcgga
    12301 ggcctcagga gtttcagctc aataggtgca ggaaaaatta tcctcctgta ctttaccctt
    12361 tcaatatgtg ctgccaaagc cgtccttctt ctggtctcct tcgatggtac acctgtttcg
    12421 atgccggttc agccattttt cagcctcttc ggacagggga tcggcaacgg cctgggttcg
    12481 ggcgcttctc tggtcaccgg cgttctttgc acccagattt acattcaggc tgttttttcc
    12541 gcatcatccc cctcgacagc aagaaaagga gccattctct ctgccatact
//
LOCUS       DAOTIQ010000055        12499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_6021_length_12499_cov_7.354950, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000055 DAOTIQ010000000
VERSION     DAOTIQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_12499_cov_7.354950"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..227
                     /locus_tag="P5541_07290"
     tRNA            142..227
                     /locus_tag="P5541_07290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Leu,seq:cag)
     gene            400..4020
                     /gene="rpoB"
                     /locus_tag="P5541_07295"
     CDS             400..4020
                     /gene="rpoB"
                     /locus_tag="P5541_07295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRV71826.1"
                     /translation="MAELITVNNRRQRLTFGRARDLVEIPDMVSIQRDSFRWFFQFDD
                     NGGLVDSEERTVHGLQELFDEIFPIESYDGSFALEFVDYFIDPPQITQEDSCQRDLSW
                     SRSVRATIRLVNRKTEEIKEEEVYLGDFPMMTDRGTFIINGTERVVVSQLARSAGVYF
                     SSEESVPGQEKYAAKVIPDRGAWLEFDMTPGELLSVNIDNRKKLPVTILLKAFGIENN
                     DGILKVFGAVEEQADLVEDEVRGRLVAETINDDSGKPLIRKNERITKEILEKLWEMGR
                     TRVWIWRTDPSIEATLDRDETTNRDEAVMELFRKLRPNEPARMENANEYVNGLFFDTR
                     RYNLGRVGRYKLNRRLQLDMPESKRLLTIEDVVQIVRKLMALRDGDERTDDIDHLGNR
                     RVRAVGELLQNQVRVGLLRMERIARERMTTIPDLTGATARDLINVRPVSAALREFFGS
                     GQLSQFMDQTNPLAELTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEG
                     PNIGLVTSLSIYSRINEYGFLQAPKRRVVGGLVTDEVVYLGADEEDEVYIGRANTPIN
                     EDGSFAEKEVYVRYRGDIAEVVPEEIDYLDLSPKQIVSASTALIPFLEHDDANRALMG
                     SNMQRQAVPLIRAEAPVIGTGIEARVARDSGSCVVARSSGTVVKVDARQIEVLPEGGL
                     EPQTYNLIKFRRSNQGTIIHQKPLVGKGDIVRKGDVIADGQAVENAELALGTNVLVAF
                     VSWEGYNYEDAILLSDRLVKEDKFSSIHIEEYEVEARDTKLGPEEITRDIPNVGEDAL
                     KDLDDTGIVRVGAEVVAGDILVGKVTPKGESDQSPEEKLLRAIFGEKAREVRDTSMRI
                     PHGEGGKVVAVKVLSRSENGDELSPGVNEVVKVYVARIRKITVGDKMAGRHGNKGVVS
                     RILPVEDMPYLPDGTPIDVVLNPIGVPSRMNLGQVLETILGFVATQNGMHVSTAVFEG
                     AKENEILELVDEVRKEKVPELTPDGRILLYDGRTGEPMKLPVTVGTMYMMKLIHLVDD
                     KIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGASHVLQEMLTVKSDDIR
                     GRLKTYERIVKGLNLSDPGVPESFRVLVKELQGLGLDVEIAYKDGSVGELLLEEDDEP
                     SHSQSRTRPREEPKKPGVKSFEDVFEDSGAHGPAPVKEGPEGIFESPDDTDDDPEEGA
                     DE"
     gene            4021..8988
                     /gene="rpoC"
                     /locus_tag="P5541_07300"
     CDS             4021..8988
                     /gene="rpoC"
                     /locus_tag="P5541_07300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRV71827.1"
                     /translation="MNQHEITGVKIKLASPERIRGMSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTKSYECACGKYKRSGPKFKGVVCDRCGVEVADNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGTATKDLEKVVYFAPTRRRDPVMKVVIEGRRTDLIKRGDLA
                     SVCEERIHRHYDHKFKSEEANRVTHVSDVSVNAGDLVPASQVGAFKRDFGDKVCKVEP
                     AFRVAETSSDGVFQEGQILSVTEREKILTENDSLVTERAMAGDEEGFVVTGVVHLPFK
                     KGDVISQSERQLFEEKYPGRFSVTGETVTIEDPCYIVINGGSSPFQRGDIILEREQRL
                     CSAYDKEFEAGIGAEGVLALLSRLNLGELVASLREEIGETTGQKKRKLVKRLQVAEDF
                     RKSEGNPEWMVFQVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRKLQEL
                     RAPEIIIRNEKRMLQESVDALIDNGRMGKAVLGAGNRPLKSLSDLLRGKKGRFRQNLL
                     GKRVDYSGRSVIVIGPNLKLNQCGLPKQMALELFKPFVINKLVEQGLSPNVKSAKRAI
                     ERGREDVWGILEEVIKGHPVMLNRAPTLHRLGIQAFEPVLMEGKALRLHPLVCTAFNA
                     DFDGDQMAVHVPLSIEARVEAKTIMLSAKNLLSPASGKPVVTPTQDIVLGIYYVTGMI
                     DGKKGEGKSFLGIEDALSALDHGFVDVNSKIRVKYRNEWIITSPGRALFNSILPVELR
                     YINKQMGKKSLGALIDAAYDRVGQDSLVLMLDDIKSLGYRWSTISGISFGLGDVLIPP
                     QKKQIVSDALEKEEVLSAQYGMGVLTEDEYLRQKETLWSEASKEAADSILANMDQSNP
                     IKMMMESGARGSKSQVAQMAGIRGLMADPSGKIIDYPIVSNFREGLNMLEYFISTHGA
                     RKGLADTALRTAKSGYLTRRLVDVAQDLIITADDCETTQGVCIHPLLQDGKMMISLSE
                     RIIGRTSLKDITDPESGELIVAGGEMIDPSRAAMIEKAGLEEVWVRSPLTCALQDGIC
                     KKCYGMDLSSRREVLTGETVGVVAAQSIGEPGTQLTMRTFHTGGVRITGEDITQGLPR
                     IEQLFEVRRPKKVAFLSDRDGIVSEIREMEGKRKVFISSVEEGVESKSSFNIPASQPL
                     LVEEGQTVKKAQPLTEGHVDPQQLLEVEGLEAVQNYLVDNIQSVYLSQGVSVSNKHIE
                     VILRKVAPVNRVRVIEEGDSSFVSMELAWVLDLEREIAKIREDNERFLVEAFEVMNGR
                     KLVDVVGQGNIETAISLKGQVLDDKAVRQILRPGSGVSELVVEDKTGLVRVIVGAASF
                     RRAIAGLELIESVDLDEGETLDAGTILTSAQMTKVTDARPSRLLVRDIGILDEMKDRA
                     YLAENVISGDEIVAHADRVIDAVAAENIRNSNMETVKVWRSPETVSISDAIQKMLIDQ
                     YWARPILQAIDSEGNSIKDIPQFIDGSVVKGLDEGDISAVEMDGEILSRDAIVRKVLS
                     DVAYGKVLLEPVTGRQGEELVPAGSEVNNQVLDAMVEANPEEIVIRPILTQTETRRLL
                     QGVTFVRRLREEPTWKPVIHGITKAALATDSFLSAASFQQTAQVLAGAAVRGDMDSLK
                     GLKENVIIGHLIPAGTSFDSYKDVGVIENETDPEGEPGNEPPLAV"
     gene            9072..9356
                     /locus_tag="P5541_07305"
     CDS             9072..9356
                     /locus_tag="P5541_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRV71828.1"
                     /translation="MPLNKLAVPNRVVGVKAVRKKLDAGKLSVLFLARDARVSLVSGL
                     ARDARNAGIPVEWVDSMIVLGRACAISRGAAAAGVTDLRETGSPETRGYF"
     gene            9386..9757
                     /gene="rpsL"
                     /locus_tag="P5541_07310"
     CDS             9386..9757
                     /gene="rpsL"
                     /locus_tag="P5541_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRV71829.1"
                     /translation="MPTINQLIRKGREEKKVRSKAPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGFEVTSYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVDGRKQSRSRYGARRPK"
     gene            9797..10267
                     /gene="rpsG"
                     /locus_tag="P5541_07315"
     CDS             9797..10267
                     /gene="rpsG"
                     /locus_tag="P5541_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRV71830.1"
                     /translation="MPRKGHVKNRETKSDSRFQSLEIEKFINAIMLDGKKSVAEKIMY
                     GALDLAGKRLDIEPMEVFRKAMENVRPVVEVRPRRVGGATYQVPVEVQPRRAQALATR
                     WILQYSRGKKGMPMVERLAREFTDACKGEGSSVKKREDTHRMAEANRAFAHYRW"
     gene            10322..12385
                     /gene="fusA"
                     /locus_tag="P5541_07320"
     CDS             10322..12385
                     /gene="fusA"
                     /locus_tag="P5541_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRV71831.1"
                     /translation="MSGISLEKIRNIGIAAHIDAGKTTTTERILFYTGRKHKLGEVHE
                     GAATMDWMEQERERGITITSAATTCQWRDFSINIIDTPGHVDFTVEVERSMRVLDGAV
                     AVFCAVGGVEPQSETVWRQADKYRVPRIAFVNKMDRVGADFSLVLSQMKERLGAKAVA
                     LQIPIGSEDTFGGVVDLVSNKAIIYHEDLGVEPRVVDIPAELVEEAALGRDAMIESLA
                     DLDEEIMALYLDGKPVDEDLLKKVIRKNTIELAIFPVLCGSAFKNKGVQALLNSVVDY
                     LPSPMDLPPIKGVHPSTGDVIERKADPDGPLAALAFKIVVDPFVGRLVYCRVYSGTIR
                     SGNSILNTTTGSRERVGRILRMHANKREDIEEAGAGEIIALPGLKATKTGDTLTDEKK
                     PVVLETVEFPDPVIHLSVEPMSKADQAKLGKGLSGLSEEDPTFMVHTDEDTAQTIISG
                     MGELHLEIIVDRLKREYGVEVKVGRPQVSYRETVRNPSSGECKFVKQTGGRGQYGHVV
                     LEIEPLPDHKGFVFEDKIVGGAIPREYIPAVQKGISETIGSGVLGGFPVIGIRVALVY
                     GSYHEVDSSEMAFKMAASMAFKDAMRSASPVLMEPIMDVEVVTPDEYVGDIMGDLSSR
                     RGHVEGMEMRGNARIVKSFVPLSEMFGYATDLRSKTSGRASYSMQFSHYEEVPRDVTE
                     KVLKH"
BASE COUNT         3177 a         2997 c         3755 g         2570 t
ORIGIN      
        1 ttcaccgttc accgtttacc gttcgcgctc ttgaatctgg tttttcgatt cacgactcac
       61 gattcacgtt ttttgcgatc ttctccttgc caaaagaact tggcgttgct aaaatcaacg
      121 ccgttgatcc ggaatatgaa tggggaagtg gcggaacggt agacgcgctg gcttcaggag
      181 ccagtgtccg caagggtgtg ggggttcgaa tccccccttc cccaccatag ttgcgatcca
      241 acccggaaaa gatatggggt ggagcgatgg atacggtgaa agaaaaccga aagggttgtg
      301 ctgtgcctat tgacacagcg cggccctttc gttatccttc tagtttgtca cgtcttgttc
      361 caccaaatcc cctaaagaca gggagggtgt gcttctccca tggccgaatt aatcacagtc
      421 aataaccggc ggcagcgtct aacctttggt cgcgcccggg atcttgtcga gatcccggac
      481 atggtgtcga tccagcgcga ttcattcagg tggtttttcc agttcgacga caatgggggt
      541 cttgtagatt ccgaggaaag gaccgtccac gggttgcagg aactcttcga cgagattttc
      601 ccgatcgaga gctacgatgg ctcctttgcc ctcgaatttg tcgattactt tatcgacccg
      661 ccacagataa cccaggagga ttcctgccag agggatcttt catggtcccg ctctgtcagg
      721 gccacaatca ggctcgtcaa ccgcaagacc gaggagatca aggaagagga ggtctacctc
      781 ggggatttcc ccatgatgac agacaggggc acgtttatta tcaatggcac cgaaagagta
      841 gttgtcagcc agttggcccg ttccgcaggg gtttatttct catccgagga atccgtgccg
      901 ggtcaggaaa aatacgctgc caaggtaatc ccagacaggg gtgcctggct cgaatttgac
      961 atgactccag gtgaactttt gtccgtcaac atcgataacc ggaaaaaact tccggtcacg
     1021 atccttctca aagccttcgg aatcgagaac aatgatggca tcctgaaggt tttcggagcc
     1081 gtcgaggaac aggccgatct ggtcgaggac gaggttcgtg gcagacttgt cgccgagacg
     1141 ataaacgatg attcaggtaa acctcttatc agaaagaatg aaagaataac aaaagagata
     1201 cttgaaaagc tatgggaaat gggcaggacg agggtctgga tatggcggac agacccctcg
     1261 atcgaggcga cactggaccg tgatgagaca actaaccggg atgaagccgt aatggagttg
     1321 ttccggaaac ttcgacccaa cgaaccggcc cggatggaga atgccaacga atacgtcaac
     1381 gggctcttct tcgataccag gaggtataac ctcggccgtg tggggcgcta caaactgaac
     1441 cggagacttc agctggatat gcccgaatcg aagcggcttc tcactattga ggatgtcgtc
     1501 cagatcgtca ggaaactcat ggctctccgg gatggcgatg aaaggaccga cgacattgac
     1561 catcttggca accgcagggt cagggccgta ggagaactgc ttcagaatca ggtgagggta
     1621 ggccttttga gaatggagag gatagccagg gagagaatga ccaccatccc tgacctcacc
     1681 ggtgctacgg cgagagacct tataaacgtc cgtcccgtgt ctgccgccct gagagagttt
     1741 ttcggctccg ggcagttgtc gcagttcatg gaccagacaa acccactggc agaacttact
     1801 caccgcagaa ggctttccgc tctggggccg ggtggtctga gcagggagag agccggtttt
     1861 gaggcccgtg acgttcatta cacccactat ggacgtgttt gccccataga gactcccgaa
     1921 ggccccaaca ttggtcttgt tacatctctt tcgatctact ccaggattaa cgaatacggg
     1981 ttccttcagg ctccaaaaag aagggtagtc gggggtctgg tcaccgacga agttgtttat
     2041 ctcggagcgg atgaagagga tgaagtatac attggaaggg cgaacactcc catcaacgaa
     2101 gacgggtctt ttgccgaaaa agaggtctat gtccgttaca ggggcgatat tgccgaagtc
     2161 gttccggagg agatagatta cctcgacctt tcgccgaaac agatcgtctc cgcctcgacg
     2221 gcgctgatcc cgttcctcga acacgatgac gcaaaccgtg ctctcatggg gtcaaacatg
     2281 cagaggcagg ccgtgcctct tatccgtgcc gaggctccgg tcatcgggac cggtatcgaa
     2341 gccagggtcg caagggactc gggctcatgc gtggtggccc gttcttccgg aacagttgta
     2401 aaagttgacg caaggcagat agaggtactt ccagaaggtg gcctggagcc acaaacctat
     2461 aaccttatca agttccgcag atccaaccag ggaacgataa tccaccagaa accactggtc
     2521 ggcaagggtg atattgtcag gaaaggtgac gtgatcgccg atgggcaggc agtggagaac
     2581 gcggaacttg ccctcggcac caacgtcctg gtcgcgtttg tatcctggga gggttacaac
     2641 tacgaggatg ccattctgct cagtgacagg ctggtaaagg aggacaagtt ctcttcgatc
     2701 catatcgagg agtatgaggt cgaggcgagg gacaccaagc tgggcccgga ggagataacc
     2761 agggacattc cgaatgtcgg cgaggatgcc ctcaaggatc tggacgatac cggcatagtc
     2821 agggtcggag ccgaggttgt ggccggagac attcttgtcg gaaaggtcac gccaaaggga
     2881 gaatcggacc agtctccgga ggagaagctc ctcagggcca tatttggaga gaaggcgaga
     2941 gaagtccgcg acacttccat gagaatacct catggcgagg gaggcaaggt cgttgcggtc
     3001 aaggtcctca gtcgctccga gaacggtgat gagctgagcc ccggggtcaa cgaggtcgtc
     3061 aaggtctacg ttgccaggat aagaaaaatt accgttggcg acaagatggc gggaaggcac
     3121 gggaacaagg gggttgtctc caggatcctt cctgtggagg atatgcccta tcttcctgat
     3181 ggaaccccca ttgacgttgt gctcaacccc ataggtgtgc caagccgcat gaaccttggg
     3241 caggtgctgg agaccattct gggtttcgtg gccacccaga acggaatgca tgtttccacg
     3301 gccgtcttcg agggagccaa ggagaatgag atacttgaac tggtagatga ggtcaggaaa
     3361 gagaaagtcc cggaactgac ccccgacgga agaatacttc tctatgatgg ccgtaccgga
     3421 gaaccaatga aacttcccgt cacggtcgga acgatgtaca tgatgaaact tatccatctc
     3481 gttgatgaca agatccacgc aaggtccata ggaccataca gccttataac ccagcagccg
     3541 ctcggaggga aggcccagtt tggcggccag cgcttcgggg agatggaggt ctgggccctc
     3601 gaaggatacg gcgcatccca cgttctgcag gagatgctga cggtcaagtc cgatgatatc
     3661 agggggcggc tcaagaccta tgaaaggatc gtgaaaggcc tcaatctttc ggatccggga
     3721 gtgcccgaga gcttcagggt gctggtcaag gaacttcagg gcctcggtct ggatgtggag
     3781 atagcctaca aggacggttc ggtaggtgaa cttctccttg aagaggatga tgaaccatct
     3841 cattcacagt ccaggacacg gcccagggaa gaacccaaaa agccaggcgt aaaaagcttt
     3901 gaggatgttt tcgaagattc cggggcccac ggtcccgcac cggtgaaaga gggcccggag
     3961 gggatattcg aatctccgga tgacacggat gatgacccgg aagagggggc tgatgaatag
     4021 atgaaccagc acgaaataac cggtgtgaag atcaagctgg ccagtcccga gaggatcagg
     4081 gggatgtcca gtggtgaggt aaagaaaccg gagaccataa actaccggac cctgcgtccc
     4141 gagaaagatg gtctcttctg cgaaagaata ttcgggccca cgaagagtta cgaatgtgcc
     4201 tgcggaaaat acaagaggag cggccccaag ttcaagggtg tcgtctgcga caggtgtggt
     4261 gtcgaagtgg ccgataaccg tgtccgccgc gagagaatgg gacacataga gcttgcggca
     4321 ccggtggtcc atatctggta cctcaggggt attcccagca ggctgagcct tcttctggga
     4381 actgccacca aagacctgga gaaggtcgtc tactttgcgc cgacgcggag aagggacccg
     4441 gtgatgaaag ttgtcatcga gggcaggagg accgacctca taaaacgtgg ggaccttgcg
     4501 tccgtatgcg aggagaggat ccacaggcac tacgatcaca agttcaaatc agaggaggcc
     4561 aaccgggtaa cccatgtcag tgacgtatct gtcaatgcgg gtgaccttgt cccggcttcc
     4621 caggtagggg ctttcaagag agattttggc gacaaggtct gcaaggtgga gccggccttc
     4681 cgtgtggcag agacatcttc agatggcgtt ttccaggagg ggcaaatact ctcggtgacg
     4741 gagagggaaa agatcctgac cgaaaacgat tctctcgtga ccgagagagc catggccgga
     4801 gacgaagagg ggtttgtcgt aacgggagtg gtgcatcttc ccttcaaaaa gggggacgtt
     4861 atctcccaga gtgaacgcca gcttttcgaa gagaaatatc ccggacgttt cagcgtaacc
     4921 ggtgaaactg tgacaataga agatccctgc tatatagtca tcaacggagg atcctcaccc
     4981 tttcagcgtg gagacattat tctcgagagg gagcaaaggc tctgctccgc ctacgacaag
     5041 gagttcgagg ccggaatagg cgcggaaggc gtacttgcgc tactatccag gctcaacctg
     5101 ggtgaactgg tcgcttccct gagggaagag atcggtgaga caacgggaca gaagaaacgc
     5161 aaacttgtga agcgacttca ggtcgcggag gatttccgca agagcgaggg taatcccgag
     5221 tggatggtgt tccaggtcct gcccgtcatt ccccctgacc tgagaccgat ggttcagctg
     5281 gacggcggca ggttcgccac ttccgatctt aacgatcttt accggcgggt tataaaccgc
     5341 aacaacaggc tgagaaaact gcaggaactg agggcacccg aaataataat ccgcaacgag
     5401 aaacgcatgc ttcaggagtc ggtagatgcc ctgatcgaca acggcagaat gggcaaggcc
     5461 gttctcggtg ccggcaacag gcctctgaaa agcctttcgg accttctcag gggaaagaag
     5521 ggaaggttcc gccagaacct tctcggcaaa cgtgttgatt attccggcag atcggtaata
     5581 gtcatcggcc cgaacctgaa acttaaccag tgcggcctgc cgaagcagat ggctctggaa
     5641 cttttcaagc cctttgtgat caataaactt gtagagcagg gactgtcccc caacgtaaag
     5701 agcgcgaaac gagctatcga aagaggaagg gaagatgtct ggggtatcct cgaagaggtc
     5761 ataaagggac atccggtaat gttgaacagg gccccgaccc ttcaccgact gggcatccag
     5821 gctttcgaac ctgttctgat ggagggaaag gcccttcgtc tgcaccccct tgtctgcacg
     5881 gctttcaacg ccgactttga cggggaccag atggccgttc atgttcccct ctcgatagag
     5941 gcgagggtgg aggcaaagac gataatgctc tcggcgaaga acctcctttc ccccgcaagc
     6001 ggtaagccgg tcgttacacc gacccaggac atagtccttg gaatatatta cgtcactgga
     6061 atgatcgacg gtaaaaaagg cgaggggaaa agtttccttg gcatcgaaga cgcgctttcg
     6121 gccctggatc acggctttgt ggacgtcaac agcaagataa gagtcaaata caggaacgaa
     6181 tggatcatca cttctcctgg aagagctctt ttcaacagta tccttcccgt ggaactaagg
     6241 tatatcaaca agcagatggg gaagaagtcc ctcggcgccc tgatagacgc ggcgtacgac
     6301 agggtgggac aggattctct cgtgttgatg ctcgacgata tcaaatccct cggctacagg
     6361 tggtccacaa taagcggtat cagtttcggt cttggcgacg ttctcatccc gccgcagaag
     6421 aaacagatag tgtccgatgc actggagaag gaagaggtac tctcggcgca gtatggcatg
     6481 ggagtcctga cggaggacga atatctgagg cagaaagaga ccctgtggtc cgaggcctca
     6541 aaagaagcgg cggattcaat actggcaaac atggaccagt cgaacccgat caagatgatg
     6601 atggagtccg gggccagggg aagcaagagc caggtcgccc agatggcggg tatcaggggg
     6661 ctgatggctg atccttccgg aaagatcata gactacccga tagtttccaa cttccgtgag
     6721 gggcttaaca tgctcgaata cttcatctcg acccacgggg caagaaaggg tcttgccgat
     6781 acggcgcttc gtacagccaa gtcgggctat cttacgagaa ggctcgtcga tgttgcgcag
     6841 gatcttatta ttaccgctga cgactgcgaa acaacccagg gggtctgcat ccacccgttg
     6901 ctccaggatg gcaaaatgat gatatccctg agcgagagga tcatcgggag aacatcgctg
     6961 aaagatatta ccgatcccga aagcggtgaa ctgatagtcg ccggaggtga aatgatcgat
     7021 ccttccaggg cggcaatgat agaaaaggca ggcctcgagg aagtctgggt aaggagccct
     7081 cttacctgtg ctctccagga tggcatttgc aagaaatgct acggcatgga tctctcgtca
     7141 cggcgtgaag tacttaccgg agagaccgtt ggtgttgttg cggcccagtc tataggggag
     7201 cccggtaccc agcttacgat gaggacattc catacaggtg gggtccgtat caccggtgag
     7261 gacattactc agggtctccc gcgaatagaa cagcttttcg aggtaagacg tccgaaaaag
     7321 gttgccttcc tctcggaccg tgacggaatt gtttccgaaa taagagagat ggaagggaag
     7381 cgcaaggtgt ttatctcttc agttgaagaa ggggttgaat ccaagtcatc cttcaacatt
     7441 ccggcatcgc agcccctgct tgtcgaggaa gggcagacag tcaagaaggc gcagcctcta
     7501 accgaaggcc atgtcgatcc acagcagctt ctggaagttg aaggcctcga ggctgtccag
     7561 aactacctgg tcgacaacat tcagtctgtt tatctctccc agggggtatc tgtgagcaac
     7621 aagcatatcg aggtcatatt gcggaaggtc gctcccgtaa acagggtgag ggtgattgaa
     7681 gagggggaca gttcctttgt gtcgatggaa ctggcctggg tattagatct ggagagggag
     7741 atagccaaga tacgtgagga caacgagcgc ttccttgttg aggctttcga ggtcatgaat
     7801 ggccggaaac tggttgacgt cgtcgggcag ggcaacatcg aaacagcgat ctccctcaag
     7861 ggacaggtcc tggatgataa ggctgtccgg cagatactgc ggccaggttc gggagtttcg
     7921 gaacttgtag tcgaggacaa gacaggtctt gtccgtgtga tcgtgggcgc agcctccttc
     7981 aggcgtgcca tagctggcct ggaacttatc gagagcgtcg acctggatga aggggagaca
     8041 ctggatgccg gaacaatcct cacttcagca cagatgacga aagttaccga tgccaggcct
     8101 tccaggttgc tggtcaggga tatcgggata ctcgacgaaa tgaaggaccg tgcatatctc
     8161 gccgagaacg tgatatccgg tgatgagatc gttgctcatg ctgacagggt catcgatgct
     8221 gttgctgccg aaaatatcag gaacagcaac atggagacgg tgaaagtgtg gcgctctccc
     8281 gaaacggtga gtatctctga cgcgatccag aaaatgctta ttgaccagta ctgggccagg
     8341 ccgatactcc aggcgataga ttccgagggc aattcaataa aggatattcc ccaattcatt
     8401 gatggcagtg tagtaaaggg tctggatgag ggagacatat cagccgtcga aatggacgga
     8461 gagatccttt ccagggatgc tatcgtaagg aaggttctaa gtgatgtcgc ctacggcaag
     8521 gttctcctgg aacctgtaac cggcagacag ggagaagaac tcgtccccgc cgggagcgag
     8581 gtcaacaatc aggttctcga tgctatggta gaggctaacc ctgaagagat agtaataaga
     8641 ccaatattga cccagactga gacaaggagg ctcctccagg gagtgacttt tgtcagacgc
     8701 cttcgcgagg aaccaacctg gaagccggtc atccacggaa taaccaaggc cgcccttgcc
     8761 accgacagct tcctgagcgc ggcatcgttc cagcagaccg cccaggtcct tgcaggagct
     8821 gcggtgagag gtgacatgga tagcctcaag ggcctcaagg aaaacgttat aatagggcac
     8881 ctgatccctg cgggaacatc ttttgattcc tacaaggatg tcggggttat cgagaatgaa
     8941 acagatccgg aaggagagcc agggaacgag cctccgcttg cggtatgatc ctgactcgtg
     9001 gtttttttcc ttgacacgct cctttgccgt tgttattatt ccttggtgtc cttttcaagg
     9061 gggggttttc tgtgcccttg aacaagttgg ctgtgcccaa cagggtggtc ggggtgaagg
     9121 ctgtcagaaa aaaactcgac gcgggaaagt tgtcagtgct tttccttgcg agggatgctc
     9181 gagtcagcct tgtctcaggc cttgccaggg atgcgcgtaa tgcgggaatc cccgttgaat
     9241 gggtcgacag tatgatcgtt ctcggtcgtg cctgcgcgat cagtcgggga gcggctgcgg
     9301 ccggtgtgac ggacttgcgg gaaacaggat ctccggaaac ccggggatat ttttaaaacg
     9361 gcatacctaa gaaggaggga gttttttgcc gacaatcaat cagttgatcc gaaaagggcg
     9421 cgaggagaaa aaagtacgtt ccaaggcgcc tgctcttcag ggcaatccgg cccgtagagg
     9481 ggtttgtacc agggtatata ctgttactcc caagaagccg aactcggcac tcagaaaagt
     9541 tgcgagggtt cgtcttacga acggtttcga ggttacttcc tatattccgg gagtagggca
     9601 caaccttcag gaacactcgg tcgtgctggt tcgcggaggg cgtgtaaagg acctcccggg
     9661 cgtgcgttat catatcatca ggggaacgct cgactgcggc ggcgtcgacg gcagaaagca
     9721 gagccgttcg aggtatggcg ccaggcgtcc caagtaacca taatctgtaa acgtcagcag
     9781 ggagagtgtc gacgatatgc caagaaaggg acacgtaaaa aacagggaga caaagagcga
     9841 ttcaagattt cagagtcttg agatcgagaa gttcatcaat gccataatgc ttgacggcaa
     9901 gaaaagcgtg gcggagaaga ttatgtacgg cgcgcttgat ctggccggca aaaggctaga
     9961 catagaaccc atggaagttt tcagaaaggc catggagaac gtcaggcctg ttgtggaagt
    10021 gcgcccgagg cgagtcgggg gagccacgta ccaggttccc gtcgaggttc agcccagaag
    10081 ggcgcaggca cttgcgacga gatggatcct tcagtactcg agggggaaaa agggaatgcc
    10141 catggtggag agactcgcca gggaattcac cgacgcctgc aagggcgaag gaagttcggt
    10201 caaaaaacgc gaagataccc acagaatggc cgaggccaac agggcattcg cgcattatcg
    10261 ctggtaacgg aaggtttttc aggggtgaat ccgcgcaggc gctgggaatg gtgaatagga
    10321 aatgagcggt attagtctgg aaaagatcag aaatataggt attgcggctc atattgacgc
    10381 cggaaaaaca acgacaacgg agcgcatcct tttctatacg gggcggaagc ataagcttgg
    10441 agaagtccat gaaggtgctg ccactatgga ctggatggaa caggagcgcg agcgcgggat
    10501 cactatcacg tctgctgcta caacgtgcca atggagagat ttcagcatca atattattga
    10561 tacgcccggg cacgtggatt ttaccgtgga ggtcgagagg tccatgagag ttctcgatgg
    10621 agcggtggct gtattctgtg cagtgggcgg cgtagagccc cagtccgaaa cggtctggcg
    10681 tcaggctgac aagtatcgtg ttcccaggat agccttcgtg aacaagatgg accgggtcgg
    10741 tgccgatttc agcctcgttc tgtcacagat gaaggaaagg ctcggggcaa aggccgtcgc
    10801 actccagata ccaataggga gcgaggacac cttcggtggc gtagtggatc ttgtaagtaa
    10861 caaggccatt atctaccatg aggaccttgg cgttgaaccc agggtggttg acataccggc
    10921 ggaacttgtg gaggaagcgg ccctgggaag ggatgccatg atagaatcgt tggccgatct
    10981 ggatgaagag atcatggctt tatatcttga tggaaaaccc gttgacgagg atctgctaaa
    11041 aaaagttatc cggaagaata ccatagaact ggccattttc ccggttcttt gcggttctgc
    11101 cttcaagaac aagggtgttc aggctctttt gaattcagtt gtggattacc tgccgagccc
    11161 aatggatctt cctcccatca agggcgtcca cccttcgacg ggggacgtta tcgagaggaa
    11221 ggctgacccc gatggtcctc tggcggctct tgcgttcaag atcgtggtgg accccttcgt
    11281 gggcagactc gtttattgca gggtctactc agggacgatc aggagcggga acagtatcct
    11341 gaatactacg acagggagcc gcgaaagagt aggacgcatc ctccggatgc acgccaacaa
    11401 gagggaagat atcgaagaag ccggtgcggg tgagatcata gctcttcctg gacttaaggc
    11461 aaccaagacg ggagacacgc tcacggacga aaaaaaaccg gtggttctgg agacagtgga
    11521 gttccctgat ccggtcattc atctttcggt ggaaccgatg agcaaggcgg accaggcgaa
    11581 actgggaaag ggtctgtcgg gcctttctga agaggatccc acattcatgg ttcacacaga
    11641 tgaggatacc gcccagacca taatatccgg gatgggcgag ctccacctgg agataatcgt
    11701 ggacaggctg aaacgcgaat acggggtgga agtaaaggtt gggcgccccc aggtttccta
    11761 cagagaaacc gtcaggaacc cctcatccgg ggagtgcaag tttgtaaaac agaccggtgg
    11821 aagagggcag tatggacatg tggttctcga aatagaaccg ctccctgatc acaaggggtt
    11881 cgtctttgag gacaagatcg tcgggggagc aatacccaga gagtatatcc cggcagtcca
    11941 gaaaggtatt agcgaaacga taggcagcgg ggtcctggga ggtttcccgg taatagggat
    12001 cagggtggcc ctcgtttacg gcagttacca cgaggtcgac agttctgaaa tggctttcaa
    12061 gatggcagct tcgatggcct tcaaggatgc gatgagatca gccagcccag tgcttatgga
    12121 gccgatcatg gatgtcgagg tagtgacacc cgatgaatat gtcggcgata tcatgggtga
    12181 tctttcctca agaaggggcc acgttgaggg tatggagatg aggggcaacg ccaggatagt
    12241 caaatccttt gtgcccctct cggaaatgtt cggttacgct accgatctca ggagcaaaac
    12301 ctccggaagg gcgtcctatt cgatgcagtt ctcgcattac gaagaggtcc cgagggatgt
    12361 tacggaaaaa gtactgaagc actagaacta gaaacggaaa agaaagcaaa aaaatcagga
    12421 agaaacaata taggggaggg tttcaaaaaa tggcaaaaga gcattttgac aggtccaagc
    12481 cgcatctgaa catagggac
//
LOCUS       DAOTIQ010000056        11807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_6463_length_11807_cov_7.632488, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000056 DAOTIQ010000000
VERSION     DAOTIQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_11807_cov_7.632488"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..915)
                     /gene="dapF"
                     /locus_tag="P5541_07325"
     CDS             complement(85..915)
                     /gene="dapF"
                     /locus_tag="P5541_07325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRV71832.1"
                     /translation="MNYEGLKFTKMQGNGNDFIIIDNRSRSFGDDSLRDMAINLCRRR
                     ISIGADGLMAIEKSDDADFKMRLFNCDGSEGEMCGNGARCLARCAFEKGIAPKKMTIE
                     TLAGPVSAQVTPPYATLDMGLLDMTSVERGQNKNISGEDFPYSHLTVGVPHTVIFQDP
                     GDRRSPGDLAPIAIVFQSDRDLFPLGTNVNFLKVSGPNLLDVRTRERGVNDFTLSCGT
                     GSTAAAAAAWMEGLVSSPVDVTNPGGVNTVLIEENEPGVARLFLRGLTAFVAEGSVLS
                     "
     gene            complement(932..1969)
                     /locus_tag="P5541_07330"
     CDS             complement(932..1969)
                     /locus_tag="P5541_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV71833.1"
                     /translation="MTGEEPGQETLLTGFERAMALAGAATLKEALPICLEALLEAAGE
                     HLDCGWVYLLNETSDTFTLRCSFGVGQSFSDANRSWAPDSVQGRALLRNIHWYGQFHD
                     VFGERDTLLSSEGLRALAVLPVFRKDVVEGCFCLASHSFETVPVNIRCDLEALSRWFD
                     KTISRLQVSESRIRESWDLLALFDAMAEMVFAFGLDGEILWMNRTARDELGFERDDAK
                     RVHLLDIHPTEYHPDAARTFRRMLSREQQTSALPFLKRNGSVVHATMLGVPGRWRGEE
                     VLFGISSINETSFPVAGDMALALASIRKEIGMIAAKFDERLSRIEKAVLADEETGEGL
                     VSAARKTGINP"
     regulatory      complement(2061..2147)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2171..3139)
                     /locus_tag="P5541_07335"
     CDS             complement(2171..3139)
                     /locus_tag="P5541_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71834.1"
                     /translation="MELDFIKGHMGGNTIILLDGRQVPDESDVLETALKVLDRLHLCA
                     DQAGFIYPSKGENCLRALIVDITNRDFIPACGGFTQVLGRALVDTDLASRFGITLTEP
                     LTKVTLELASGKSSIAIHIRKGSFVKVVSDMTSFARMLLEDGVAEVDLDGIKAFKSGY
                     YLVQGAEDLSENYLGADFEQMNQSAVKALSEAQALFRQKKYSSSLHLSLFDTSPKVGG
                     DLRAVFPHGIATGNIEPTCGTGSTALALALLVTGEGEKLGLVKDNKLTVKLETGGDPV
                     IGGPDITTVEVRMQGSEVSEAAFSHSFTEITAQGKILVWTGADSGV"
     gene            complement(3586..4803)
                     /locus_tag="P5541_07340"
     CDS             complement(3586..4803)
                     /locus_tag="P5541_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HRV71835.1"
                     /translation="MRRNGIFLCAALLFSALLCGTALGAGFALYDFSARGNALGGAMV
                     GRADDPSAIAYNPAGITQIPGSSYMTGLAFIMPRGTVTQVGGSAVDMKEHTFIPPHFY
                     YTRQMSDKVWFGVGMMTRFGLGSDYPKTWFGNENNYYANIESVSLNPNIAWKVNDSFS
                     LSLGAEVLWMEYSQNKMGTSSAYDAQLKGDDIGYGWNVGMHFKLDEATRIGLHYRSQV
                     KLTISGTVDTQSAIPIFQDHTSAKGDITLPEMYMFGISKQFSPKTNIEVGAIYTGWSS
                     YDEMTIRYDPPLFGSVPSKTSPKNYKNVWRYQLGIEYKKSPEWTWRFGYTFDQSPIPD
                     STIDYQAPMSDRHLLSVGFGYTKNDRTWDFAYTYLMADDRTIAARPGDGVLASQTSNM
                     DANIFVVSYSVKF"
     gene            complement(4891..5496)
                     /locus_tag="P5541_07345"
     CDS             complement(4891..5496)
                     /locus_tag="P5541_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV71836.1"
                     /translation="MKDREETRMKILQAVGNVLSSEGFQGIGINNIARRAGVDKVLIY
                     RYFGDLPGLLREFASSGDYWPSMSELIEGAEKASAPPDAGDLAVALLKGYIREIGGSA
                     SAQELLLGELVSRNELSEASARKRERQGADLIEALVSAAPDAGGRDVAAMGALLSAGL
                     TFLMLRARTSDAYLGIDISSGEGWERIERAMEELVRGVLRS"
     gene            5644..7224
                     /locus_tag="P5541_07350"
     CDS             5644..7224
                     /locus_tag="P5541_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV71837.1"
                     /translation="MMDKKFYPWQIEAFDRVKGRDAVLAAPTGSGKTWVAYLWAGLMD
                     MEGNTWPPGSKRVIFTAPIKALSNERYMELRRMGLDVGIETGDFKKNESAPVICCTQE
                     IYSLKYAGTGGLKVVVDEFHYIFDDPDRARTYIEGIRDTHPDSPILVMSATFGGAQSV
                     GDYLDRITGRRFEVYEGRERVTKLVFTPRKPARFERLHDALVFVFSQKGAVDLAYKIA
                     RKRERKGKAHRERLYELASILDVDNVHMPLLRGVGVYHGGMLPKEKLLVESAFRERIL
                     DVVCGTNALALGVNLPAEYVVFAQLVRYHDREPISRNEFSQMAGRAGRKGLFDTGFVT
                     WLKSSPFESRGVDTGAVFRHLVGAQPEKATVSLRPDYGKLLKKRRTVIDEAEYIASFS
                     LPPSDPRIIEGQLIAGLKFIERSIREMAEPEAREHFRETLADIWYGEMEIGENLEMAH
                     LFLKDGKADALTAAQVINLFEKSYLKSLLKVKRFSNHIPPGYQITGMQYLDRTVEELD
                     GTIYGFEEKIQEIEESAR"
     gene            complement(7256..8191)
                     /locus_tag="P5541_07355"
     CDS             complement(7256..8191)
                     /locus_tag="P5541_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71838.1"
                     /translation="MAKVKKTLIMMVSVVAIACLAMSVSAWEGPMVFSRRDGIIYLIA
                     EKGLTAVDVAMGLDPDIGPDGRSIVYVTEEIASDGAESSFIWIKNLENMSVRSVLKGG
                     GRIRLPEWSPDGSEIAFLMFDSEYFWSIHVVRPDGTGFRRVAGSKPGGEGPVLSGSVN
                     WWPDGKSLIAQDGLFVYRFGLDGNLMEKTPVEKFTGDKYATTSTDRFLPHPEREGLWA
                     FTMSVPGTPLFEKTFNEPNQALFIIDMASGEKKRLTPEDMLAMDINWSPDGKMIFFSG
                     YRDANMNESYPFRIYSLDVETGEITEVCPGEQPGL"
     gene            complement(8250..8873)
                     /locus_tag="P5541_07360"
     CDS             complement(8250..8873)
                     /locus_tag="P5541_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRV71839.1"
                     /translation="MAKEHKGNFEYQIKTHPRAKRVTLRVNAPEGLVIVVPKGFSKDK
                     IAEIIEEKRSWIEKALYRVLPASSREDISGPRETFFSPAFGRNLELDRNDARGSLRLL
                     AVENITPLAWKISGETGLRPEKFTIKNQKSVWGSCSTKNNINLNQKLLFLEPWLVRYV
                     ILHELCHLRYRNHSAGFWNFLAELDPRCLSHRKEIKEAQRQVPHWAY"
     gene            9041..9529
                     /locus_tag="P5541_07365"
     CDS             9041..9529
                     /locus_tag="P5541_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV71840.1"
                     /translation="MGAKNEARELREATRELFRNLSLLDRDRASCCGVTVAQCHAIVE
                     IGRLGKVSPGMLAETLRLDRSTVTRLADSLVAMDFITREQDPEDRRSLVLALTEKGEQ
                     FHSLTEQAMEDFYRSIIQKMPPEERRALITGISAISESYRTGECKCFRNEDKPAYSGE
                     GE"
     gene            9536..10348
                     /gene="arsM"
                     /locus_tag="P5541_07370"
     CDS             9536..10348
                     /gene="arsM"
                     /locus_tag="P5541_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRV71841.1"
                     /translation="MDDIREKVRKKYAEAIRNKRSCCGGSSSCCGGTEAGDPITAGIY
                     EGAVLERIPGGVEGQSFGCGSPVEMAGLKPGETVLDLGSGAGLDAFLAGGEVGVSGKV
                     FGLDMTDEMILTAESNRIHWGLENVEFLKGFIEEIPLPDESIDVVVSNCVINLSPDKD
                     AVFREIFRVLKPGGRISVSDVVFLRPIPEEIRASIDAWAGCVAGALGREEYEQKLAEA
                     GFTGISVSPTRVYDFTPSQLEEMFPDLSGAKSLDAGGVAAGAWISAMKPRPI"
     gene            complement(10286..10783)
                     /locus_tag="P5541_07375"
     CDS             complement(10286..10783)
                     /locus_tag="P5541_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71842.1"
                     /translation="MENSLSEAMERLKRQAGLLDEPIEELRSLQSRLEGEAERFLESV
                     ARAAGGRPLKFSWGSLGCVIRKGANGALCDCRVEEGDEEIGELAADLSLDLESATRVY
                     ILSHREEITEACAQALEDQRQTLRDSIDHIRSILDLGGEDPDTSDGPGLHGGDPGSGS
                     NSAGI"
     gene            complement(10818..11801)
                     /locus_tag="P5541_07380"
     CDS             complement(10818..11801)
                     /locus_tag="P5541_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV71843.1"
                     /translation="MKNQRTEKALFVSWVGLVVNVGLTIFKFIAGFAGNSAAMVADAV
                     HSLSDFATDIVAVIGFKVTGRPVDRSHDYGHGKFETLTTVIVGVTLLAVAAGIFYSGG
                     RRVLLVLKGETIPRPGMIALIAAGLSIAVKEWLYFYTKRTAIQISSDALMAKAWDHRS
                     DALSSVGTLFGIGGAIFLGPGARVLDPVAALIVAAVIVRVALPITIRSLSELLEESLD
                     AEQEDEIMGAILVVPGVEGAHHLRTRRIGANVAVEVHILVDRDLRITEAHDIATMAEK
                     AVRSIHGEDSFVSVHIEPVSEEIPESIPFDDGHNGCENGPGHHERDFKWQE"
BASE COUNT         2722 a         3330 c         3094 g         2661 t
ORIGIN      
        1 tgcccttcca gtctcctgtt tccagcttcc tgactcctgc tcttcaggtt cattcttttg
       61 ctttttcgat tccctatccc ctattcacga cagaacagat ccctcggcaa caaacgcggt
      121 cagcccacgc aggaacagcc ttgccactcc aggctcattc tcctctataa gcacagtgtt
      181 cacgcctccg gggtttgtga catcaaccgg agaggataca agcccctcca tccatgcagc
      241 agcggcagcc gccgtggatc cggtcccgca ggaaagggtg aagtcgttaa cgccccgctc
      301 ccgggtcctg acatccagta agtttggacc agaaactttg agaaagttca cgttggttcc
      361 cagggggaag aggtctctgt ctgactggaa gacgatagct atcggcgcaa gatccccggg
      421 gcttctcctg tcgcccgggt cctggaatat gaccgtgtgg gggaccccta ctgtcagatg
      481 gctatatgga aagtcctcgc ctgagatgtt cttgttctgc cccctctcga cggaggtcat
      541 gtcgagaaga cccatgtcca gggtcgcata ggggggtgtc acttgcgccg atacaggccc
      601 cgccagagtt tctatcgtca tttttttggg agcgatcccc ttctcaaacg cacaacgtgc
      661 aaggcacctc gctccgttgc cgcacatctc accctctgaa ccgtcacaat tgaagagcct
      721 cattttgaaa tcggcatcat cgcttttctc tatggccatc aggccgtcgg cgcctatgga
      781 tatcctcctg cggcacaggt taatagccat atctctgaga gagtcatctc cgaaagatct
      841 gctcctgttg tcgataatga tgaaatcgtt gccgttaccc tgcatcttgg tgaatttcag
      901 gccttcataa ttcattgaca aacctccgcc atcaggggtt gatcccggtt tttcgcgccg
      961 ccgaaaccag accttcgccg gtctcttcat ccgcaagaac ggccttttcg atgcgggaaa
     1021 ggcgttcgtc gaatttcgca gctatcatgc ctatctcctt ccgtatggaa gccagcgcta
     1081 aggccatgtc tcccgcaacc gggaaggaag tttcattgat actgcttatc ccgaaaagta
     1141 cctcctcgcc cctccatctg ccggggactc ccaacatagt ggcgtgaacc acgcttccgt
     1201 tccttttcag gaaagggagg gcagacgtct gttgctcccg ggaaagcatc cttcgaaagg
     1261 tcctggctgc atcggggtga tattccgtgg gatgaatatc cagcagatgg accctctttg
     1321 cgtcgtctct ttcgaaaccc agttcatccc tggccgtcct gttcatccag aggatctctc
     1381 cgtcaaggcc aaaggcaaag accatctccg ccatggcatc gaaaagagcc aggaggtccc
     1441 aggattctct tatcctgctt tcgcttacct gtagcctgct tatggttttg tcgaaccatc
     1501 tggacagggc ctcaaggtcg cacctgatat tgacgggaac tgtctcgaag gagtgggagg
     1561 caaggcagaa acacccttcg acgacatcct tgcggaacac agggagcact gccagcgcgc
     1621 gaagaccttc cgatgaaaga agagtatctc tctcaccaaa aacatcatgg aattgtccgt
     1681 accagtgaat atttcggaga agcgccctcc cctgaaccga gtcgggagcc caggagcgat
     1741 tggcatcgga aaaagactgg ccgacaccaa aggagcatct aagggtaaag gtatcggaag
     1801 tttcgttgag aagatacacc cagccacagt caagatgctc ccccgctgcc tccagaagag
     1861 cctcaaggca gatcggaagt gcttccttga gcgtggcggc cccggcaagg gccatggcgc
     1921 gctcgaatcc cgtcagcaga gtttcctggc caggttcttc tcccgtcatc ttcggtatct
     1981 cccggtcatg aagacttcca taaaaacaca cgcggccccc gaaaaaaggg accgcgcgat
     2041 ggattccagt atttttagaa ttcggcggcc cggcgatcat ggccctttgg ggctttagac
     2101 ccgcggcttt gcgtcaccgt ctttcggcgg ttttgccttt tcgaaacggt cgatcctttg
     2161 gtaaccccga ttatacgccc gaatctgccc ctgtccagac caaaattttt ccctgcgccg
     2221 tgatttctgt gaaactgtgc gaaaaggcgg cttctgatac ttcgctaccc tgcatccgga
     2281 cctcaacagt ggtaatgtcg gggccaccga tcacgggatc tccccccgtc tccagtttta
     2341 cggtcagttt attatccttg accaggccaa gtttttcccc ctcacccgta accagcagag
     2401 ccagagcaag ggcggtgctg ccggtcccgc aggtgggctc gatattgccc gttgctattc
     2461 cgtgagggaa gactgcccga aggtcgcccc ccactttcgg cgaagtgtca aagagggaga
     2521 ggtgcagaga gctggagtat tttttctgtc ggaaaagagc ctgagcctcc gagagcgcct
     2581 tgacggcgga ctggttcatc tgctcgaaat cggcaccgag gtagttttcc gaaagatctt
     2641 ccgccccctg gaccaggtag tacccggact tgaaggcctt gatcccgtcg aggtccacct
     2701 cggcaacacc atcctcaagg agcatccgcg caaaagatgt catgtcgctg acgactttca
     2761 cgaaagatcc ttttctgata tgaatggcta ttgaagattt ccccgaggca agttccaggg
     2821 tcacctttgt cagcggctcc gtcagggtga tgccgaaacg ggacgccagg tccgtgtcta
     2881 caagggccct acccaggacc tgtgtgaacc ccccgcaggc agggatgaag tccctgttgg
     2941 tgatgtcgac gatcaaagcc ctgaggcagt tttcgccttt ggacggatag ataaaaccag
     3001 cctggtcggc acagagatgc aacctgtcga gaaccttcaa agccgtttcc agaacatccg
     3061 actcgtccgg aacctgcctg ccgtccagaa gaatgatagt gttgcctccc atgtgtccct
     3121 tgatgaagtc cagttccata ttccttattc ctccccgggt tgcctgtgtc ctgtaatttt
     3181 acacgaaaag gcaaggagac tggagacagg agtcgggaaa ctggaagggc aaaagtctgt
     3241 cattctgagc gcagcgaaga atctgttttt tcgtgagtcg tgaatcggaa cacccgtgaa
     3301 tcgttaatcg tgagtcgtga atcggaaaac caaggagaca ggaagctgga aacaggagac
     3361 tggaagggca aagccttgtc attctgagga atgaaatgac gaagcggtat ccgccagcag
     3421 gcggacgttc cgcgcccttt gcctaccggc gggaatctga gtcagaacca gatccttcgc
     3481 tacgctcagg atgacaaagc gcacgccctc ggcctttccc gcctcatgtc tccagatgcc
     3541 agtctccttg cttttttacg cttttttccc ttgagcgcca cgcccttaaa acttcacgct
     3601 gtaactgacc acaaaaatgt tggcatccat gttgctggtc tgagaggcaa gtactccatc
     3661 tccgggacgg gcagctatcg tcctgtcatc ggccataagg taggtatagg caaagtccca
     3721 tgtcctgtcg ttcttcgtgt agccaaaacc gaccgagagg aggtgtcgat cgctcatcgg
     3781 cgcctgataa tcgatcgttg aatccggtat gggcgactga tcgaaagtgt agccgaagcg
     3841 ccatgtccat tcggggctct tcttgtactc tattcccaac tggtaccgcc agacattttt
     3901 gtagttcttt ggggaagttt tgctcggaac cgaaccaaat aagggggggt cgtacctgat
     3961 tgtcatctcg tcgtaactac tccatccagt ataaatggct ccaacttcta tgtttgtttt
     4021 tggcgaaaat tgtttgctga ttccgaacat atacatctcg ggaagggtaa tgtctccttt
     4081 tgcggaagta tgatcttgaa aaatgggaat tgcactttgt gtatcaacag ttccggaaat
     4141 tgtcaatttc acttgactcc tatagtgaag gccaatccgg gtggcttcgt caagtttgaa
     4201 gtgcattccg acgttccaac cataaccaat atcatcgcct tttagttgag cgtcataagc
     4261 ggaagaagta cccattttat tttgtgaata ctccatccat agcacctcgg ccccaaggga
     4321 gagggagaag gaatcattca ccttccaggc aatattcggg ttcaatgaaa ccgattcgat
     4381 attggcgtag tagttattct cgttcccaaa ccacgtcttt ggataatcgc tcccgaggcc
     4441 gaaccgggtc atcattccca ctccgaacca gaccttgtca ctcatctgcc gggtgtagta
     4501 aaagtgggga gggatgaagg tgtgctcttt catgtcgacc gcggaacctc caacctgggt
     4561 aaccgtcccc ctgggcatga tgaaggcgag acccgtcatg taactgctcc ccggtatctg
     4621 ggttattccc gcagggttat aagctatggc agaagggtcg tcggcgcgac cgaccatcgc
     4681 cccgccgagg gcgttgcccc tggcgctgaa atcgtagagg gcaaaccccg ccccaagcgc
     4741 ggtgccgcac aaaagagcag agaacaataa cgcggcgcaa agaaagatcc cgtttcttct
     4801 caaatgatga agcctccttt ttggatcgtt gatgggttat gggcgccgca ttttcatggg
     4861 gacagccccc tttttttagg cacgtctgct tcaggaacgc agaacacccc gtaccagttc
     4921 ttccatcgcc ctctctatcc gctcccaccc ttccccggaa gagatgtcaa taccgaggta
     4981 agcgtcggag gtgcgggcac ggagcatcag gaaggtcagc ccagccgaaa ggagggcacc
     5041 catggcagcc acatctcttc caccggcgtc aggcgctgca cttacaagag cctcaatgag
     5101 gtccgccccc tgtctttctc tttttctggc ggaggcctct gatagttcat tgcgggagac
     5161 aagttctccc agcaggagtt cctgggccga cgcagatcct ccaatttccc ttatatatcc
     5221 cttgagaaga gcgacggcga ggtcccccgc atccggggga gcagatgctt tttcggctcc
     5281 ctctatcagt tcactcatcg atggccagta gtcaccgctt gaggcaaact cccgaagcag
     5341 gccgggaagg tctccgaagt agcgataaat caggaccttg tccacacccg ccctccgggc
     5401 gatgttgttt atcccgatac cctggaaacc ctcactggag agaacattcc ccactgcctg
     5461 gaggattttc atcctggtct cttccctgtc cttcatggca cctcctgtca ccagccggtg
     5521 acaactaact atatgagatt attaccaggc acgtcaagaa gaatggggga agcaggccgg
     5581 gagtggatgt cttctttcat agtatgatta tgttttgaat acggcgtctt aaggagcatg
     5641 acgatgatgg acaagaaatt ctatccatgg cagatcgagg cattcgacag ggtaaaggga
     5701 agggacgccg ttctggccgc gcccaccggc tccggcaaga catgggtggc ctacctctgg
     5761 gcaggactta tggatatgga gggcaatacc tggcctcccg ggtcgaaacg cgtaatattc
     5821 accgcaccca tcaaggccct cagcaacgag cgttacatgg aactccgaag gatgggtctg
     5881 gatgtgggaa tagagaccgg ggatttcaaa aaaaacgaaa gcgcccccgt aatatgctgc
     5941 acccaggaga tttactccct caaatacgcc ggcactggcg gcctcaaggt cgtcgtggac
     6001 gagtttcact atattttcga tgaccccgac cgggccagga catacatcga agggatcagg
     6061 gacacccacc cggactcccc gatactggta atgtcggcaa ctttcggggg agcccaatct
     6121 gtcggggact acctggacag gatcaccggg agacgtttcg aggtctacga gggacgcgaa
     6181 agggtgacaa aactggtttt caccccccga aaaccggccc ggttcgagag gcttcacgat
     6241 gccctggttt tcgttttctc ccagaaggga gccgtggacc tggcctataa aattgcccga
     6301 aaaagggaaa gaaaagggaa agcccacagg gagaggctct acgaacttgc gagcattctg
     6361 gacgtcgaca atgtccacat gcctcttctg aggggtgttg gggtttacca cggcggcatg
     6421 ctccccaagg agaaacttct cgtcgagtct gccttccggg agaggatcct ggacgtcgta
     6481 tgcgggacca acgcccttgc ccttggcgtg aacctcccgg cagagtacgt ggtgtttgcc
     6541 caactggtcc gctaccatga ccgtgaaccc atctcacgca acgagttctc ccagatggcg
     6601 ggcagggcgg gaagaaaagg attgttcgac acgggcttcg tgacctggct gaaaagttcc
     6661 cccttcgaat ccaggggcgt cgataccggg gccgtattca gacacctggt aggggctcag
     6721 cctgaaaaag cgacggtttc cctgagaccc gattacggca aactcctgaa aaaaagacgc
     6781 accgtcatcg acgaagccga atacattgcg tccttctccc tccccccttc agacccgagg
     6841 atcattgaag ggcagttgat agcggggctg aagtttatcg aaagatccat acgggaaatg
     6901 gccgaaccgg aggcaagaga acattttcgc gagaccctgg ccgatatctg gtacggagag
     6961 atggagatcg gtgaaaacct cgaaatggcc cacctattcc tgaaggacgg aaaagccgat
     7021 gcccttaccg ccgcccaggt catcaacctc ttcgagaaga gttatctaaa gtcgcttctc
     7081 aaggtgaagc gcttctccaa ccatattccg ccgggatacc agataacggg gatgcagtac
     7141 ctcgacagga cggtggaaga actggacggg accatctacg gattcgagga gaaaatccag
     7201 gagatagagg agagcgccag gtagggctct cctctatcct atgtcaggca agatcttaca
     7261 ggccgggttg ctcccccggg caaacttccg ttatttcacc cgtttccaca tccagtgagt
     7321 agatcctgaa agggtaggat tcgttcatgt ttgcgtccct gtaaccgctg aaaaagatca
     7381 tctttccgtc cggtgaccag ttgatgtcca tagccagcat gtcctccggt gtgagcctct
     7441 tcttttctcc ggaagccatg tcgataatga acagggcctg gttcggctcg ttgaaggtct
     7501 tctcgaaaag cggcgtgccc ggaaccgaca tggtaaaggc ccaaaggccc tcacgctcgg
     7561 ggtgcggcag aaaacgatcc gtagaggtcg tggcgtactt gtcccctgta aatttctcta
     7621 cgggcgtctt ctccatcagg ttcccatcga ggccaaaccg gtaaacgaag agcccgtcct
     7681 gagcgatgag gcttttcccg tctggccacc agttgaccga gccggaaaga acggggccct
     7741 caccgcctgg tttgctcccc gcaacccttc ggaacccggt cccgtcgggg cggacaacgt
     7801 ggatgctcca gaaatattca ctgtcgaaca tcaaaaaggc aatttcagaa ccatcgggag
     7861 accactccgg gagccttatc ctgcccccgc ctttcaggac cgagcggaca ctcatgttct
     7921 caagattctt gatccagatg aaagatgact cggcgccatc gctggcgatc tcttcggtca
     7981 cgtagacaat ggatcttcca tcgggtccta tgtcaggatc cagtcccatt gccacatcca
     8041 ccgccgtaag ccccttttcg gcaatcaggt agataatgcc atcccttcgg gaaaagacca
     8101 tcggcccttc ccaggcagac acagacatgg caagacaggc aatggccacg actgacacca
     8161 tcatgatcag agtttttttc acttttgcca tagtttcacc tcccggcact attctaacct
     8221 gctgacaacc aaatttgcgt catgccccgt caataagccc aatgcggcac ctggcgctgg
     8281 gcctctttga tctccttcct gtgggacagg cagcgcggat ccagttccgc gaggaagttc
     8341 cagaatccgg cagagtgatt ccggtacctg aggtggcaaa gttcatgcag tataacgtag
     8401 cggacaagcc agggctccag aaaaagcagt ttctggttca ggttgatgtt gtttttcgtg
     8461 gaacagctcc cccagacact cttttgattc ttgatggtaa atttttccgg ccgaagcccc
     8521 gtctccccgg aaattttcca ggcaagaggc gttatgttct ccaccgctag caggcgaaga
     8581 gaccctcttg catcatttct gtcgagttca aggttcctgc cgaaagcagg cgagaaaaaa
     8641 gtctcccgag gaccggaaat atcttcccgg gaactcgcag ggagaactct gtaaagggct
     8701 ttctctatcc aggacctttt ttcctcgata atttcggcta ttttatcttt tgagaaaccc
     8761 ttcggcacca ctatcacaag accttccggc gcgtttaccc tcagggtaac cctctttgcc
     8821 cttggatgag tcttgatctg atattcgaag ttccccttgt gttctttggc caatgataaa
     8881 ttatcctccg tctctggttt tttagaaccg cacggccgag cccggtccat gatatcaccg
     8941 atccatagcc acagaatcag gacttaagaa atcttataca acaaaccgct tgcaaagctc
     9001 aatagtaggg atatacaatt actttcgctg gaggtgtgcc atgggggcca aaaatgaggc
     9061 aagagaacta agagaggcga cgcgggaact tttcagaaat ctttctctgc tggacaggga
     9121 tcgtgcttca tgctgcgggg taacggtggc ccaatgtcac gccattgtag agatagggcg
     9181 tctcggaaaa gtctctcccg gaatgctcgc cgagaccctg agacttgaca gaagcaccgt
     9241 caccaggctg gctgacagcc ttgtggccat ggatttcatc accagggagc aggacccgga
     9301 agaccggaga tccctggttc tggcattgac tgaaaaggga gaacaattcc acagcctgac
     9361 ggagcaggca atggaagatt tctacagatc gataatacaa aagatgcctc ctgaggaacg
     9421 aagggctctc attactggta tttcagccat atcggagtct tacagaacgg gagagtgcaa
     9481 atgcttcagg aacgaggata aaccagcata ttcaggtgaa ggagaataga cggtaatgga
     9541 cgatatcagg gaaaaggttc gcaaaaaata cgccgaggcc atacggaaca agagatcatg
     9601 ctgcgggggc tcatcatctt gctgtggagg gactgaagca ggggacccca tcactgcggg
     9661 catctacgaa ggagccgttc ttgaaaggat acccggaggt gtcgagggcc aatccttcgg
     9721 ttgcggcagc cccgtggaga tggcaggact taagcccggt gagactgtcc tggacctggg
     9781 aagtggcgca ggtctggacg cttttctcgc gggcggagag gtgggagttt cgggaaaggt
     9841 cttcggcctt gatatgaccg atgagatgat ccttacagcc gaatccaacc ggatccactg
     9901 gggactggaa aacgtcgagt tcctgaaggg gttcattgag gaaatacctc tccccgacga
     9961 aagcatcgac gtagttgtct cgaactgcgt cataaacctc tcacccgaca aggatgccgt
    10021 tttccgcgag atattcaggg tcctcaagcc tggagggagg atatccgtat cggatgtcgt
    10081 attccttcgc cccattccgg aggagatccg cgccagcatc gacgcgtggg cagggtgcgt
    10141 cgccggagcc cttggaaggg aagaatatga gcaaaaactt gctgaggccg ggttcaccgg
    10201 aatatcggtc tctccgacca gggtctacga tttcacaccc agccagcttg aggagatgtt
    10261 ccctgacctc tccggggcaa agtcgctaga tgccggcgga gttgctgccg gagcctggat
    10321 ctccgccatg aagcccaggc ccatctgagg tatccggatc ttcaccgcca aggtccagga
    10381 tcgaacggat gtgatcgata ctgtcgcgca gggtctgcct ctggtcttcg agcgcctgcg
    10441 cgcacgcttc cgtgatctcc tccctgtggg agagaatgta gacacgcgtc gctgattcaa
    10501 ggtcgagtga aaggtcagcg gcaagctctc cgatctcctc gtctccttct tcgacacgac
    10561 aatcgcataa agcaccgtta gcgcctttgc gaataacgca acccagagaa ccccacgaaa
    10621 atttcagggg gcgaccgcct gcagcccttg caacagattc aaggaaacgt tcagcctctc
    10681 cttccagcct gctctgaagg ctcctcaact cttcaatggg ttcatcaaga agacctgctt
    10741 gcctcttcag cctctccatg gcttcactca aagaattttc caaaagatat ctcctcactt
    10801 tatggtatca gtgatagtca ctcctgccat ttaaaatccc tttcgtgatg gcccggtccg
    10861 ttctcacagc cgttatgtcc gtcatcgaaa ggtatgctct ccggaatctc ctccgatacc
    10921 ggctcgatat gaacagaaac gaatgagtcc tcaccgtgga tacttctcac agctttctct
    10981 gccatggtcg caatatcgtg tgcttcagtt atgcgaagat ctctatcaac cagaatatgc
    11041 acctcaactg cgacattggc tcctatgcgc cttgtcctca ggtgatgcgc cccctcaact
    11101 ccaggaacta caagaatggc gcccatgatc tcgtcttcct gctcggcgtc aagggactcc
    11161 tcgagaagtt cgctcaggct cctgatcgtt atcggaagcg ccaccctcac gatgaccgct
    11221 gccacgatga gggccgcgac agggtcaagc accctggccc ctggccccag gaagatcgct
    11281 cccccgatgc caaaaagcgt ccccaccgag gagagcgcgt cagagcggtg atcccaggct
    11341 tttgccatga gagcatcgct ggagatctgg atggccgttc tttttgtgta gaagtaaagc
    11401 cattccttga cggcaatgga cagacccgcc gcaataagag ctatcattcc aggccggggt
    11461 atcgtctctc ccttgaggac aagcagcact ctcctgcctc cgctgtagaa gattcccgcc
    11521 gccacggcaa ggagcgtgac tcccactatc actgtcgtaa gggtctcgaa tttgccgtgc
    11581 ccgtagtcgt gggacctgtc gacaggacgg cccgtgacct tgaagccgat gacggccacg
    11641 atatccgtcg cgaaatcgga aagggagtgc accgcgtcgg cgaccattgc cgcgctgttc
    11701 cccgcaaaac ctgcgataaa cttgaatatg gtgagaccga cattcaccac aagcccgacc
    11761 catgaaacaa agagggcttt ttcggttctc tggtttttca atcggaa
//
LOCUS       DAOTIQ010000057        11371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_6780_length_11371_cov_7.815394, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000057 DAOTIQ010000000
VERSION     DAOTIQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_11371_cov_7.815394"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="P5541_07385"
     CDS             <1..951
                     /locus_tag="P5541_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV71844.1"
                     /translation="MAWSLTRDPDSLFAFFHSSMDVPGGYNIQGLHDEALDRVLEKLR
                     WAPDRETADKAARRAQKMLSEITPWIPIYSRYMVTAVRDGWKGIIETERTTADNIWTL
                     INMEPGNGGDRPIMWCLQEEPRSLNPFSTSSVYDWQVLGLVYDSLIAIDPEDLEDIPW
                     LARSWEVETTGSGSTAKTRLTFALRSGIRWHDGVLFTSRDVRKSLEFLKSREIPRYFD
                     NVRDIESIETPDERTVIVNMSNTSFWHLHNIGGSPVFPAHIIENVKDWESWQPSREKH
                     PSVEGLTMLIGTGPFVFREYRPGEYVHFTRFDDFRLPWRD"
     gene            955..1932
                     /locus_tag="P5541_07390"
     CDS             955..1932
                     /locus_tag="P5541_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71845.1"
                     /translation="MNSYWIRRFAGALAVLLLIITANFFLFRVMPGDAVSTIVDPRFS
                     PEAKEKMRHLFGLDLPLHQQFFIYVRRMLSFDFGISFLSQRPVWDEIKSRIPNTLALL
                     GTSLLLSSAIGTWLGVKAARKRDGFLEKAVIFSGSISFSFPSFFVQLVLLLLLAYTFP
                     VFPLRGTVSIPMPAGTLPRVIDYIHHLILPAGSLVLLGFGSWAIYVRNLMVKTLGEDF
                     ILLARARGLSEDRIIWNHAFRSTLPPILTIFLLALPGIVSGAVITETVFSLHGVGKFL
                     LDSILGHDYPSAGAAFYILGLLTIASNLAADAACTLADPRIRVGKRAER"
     gene            1937..3121
                     /locus_tag="P5541_07395"
     CDS             1937..3121
                     /locus_tag="P5541_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71846.1"
                     /translation="MPRWSFAAFFIIVAAAIAGPSLLQTDPFESVARPFAPPVWTGGE
                     EGPALAVSLSPSKTLFEKKWNWSPPENISIRGTLSFEEDPSASLVWTGPGGERFVLAV
                     SGDTREWLIDIDARDVSFKASLGVPPFGNATSVLFRNHENIRVEIEGTTPSAGKIEIR
                     TPGGKWGLMGTDQRGRDVLSLFTEGIRISLIVGLAATLISSALGLLVGLSSGYLGGKI
                     DSFIMRGVDVLLSIPVLPILMVIVGIWGKGLWQLILILGLFSWMGTARTVRSLALTLR
                     ESCYVENLRSIGASPWYILWRHLVPEALPVLLAAVTLGVPGAILAEAGLSFLGLSDPR
                     IISWGRMLHEAHGFGAFTAGAWWLILPPGIGISVICLVFLDMGRALEEIADPRLVKGN
                     RK"
     gene            3118..3903
                     /locus_tag="P5541_07400"
     CDS             3118..3903
                     /locus_tag="P5541_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71847.1"
                     /translation="MICIRDLSVTYNTNARKVRAVREASIDIHHSAVTAIVGESGGGK
                     SSLIYSILGLLPPEATATGKILFEGSDILSAPEATRRDFRGIKVALVTQGAMNSLTPV
                     ISVGEQIAETLRTRLGTRRREAAVKASELLEMVGLGPSFAVRFPHELSGGEKQRVVIA
                     LAISCSPVFLLADEPTSALDVITQAEIITLLGGMVRETGMGLLLVTHDLPLASSVSDS
                     ISVMHQGKIVESGPAEKIMTSPLHPHTRDLVFAANLATGRGFQ"
     gene            3900..4835
                     /locus_tag="P5541_07405"
     CDS             3900..4835
                     /locus_tag="P5541_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71848.1"
                     /translation="MRSPLLVAQKITVSFGSARNQVQALAGTDLEIFPGESLAIVGES
                     GSGKTTLLRACLGLTAISGGYASLFGKDLSLCSRGELVFLRRRSGYVPQDPFGCVPPT
                     LSALDAVSEPMLIADSPGKKRASERAGELLVECGLADREIWKERVRFSLSGGQRQRVS
                     IARALALDPELLLADEPASMQDAATRKGIMEILRKRCEKGMGLVMATHDLLLASASAS
                     RVMVLYRGHVVETGPSKDLLSTPAHPYTRALGAALPGMGHSPQILVRRTDSLSGKTGC
                     VFSARCPRRTEKCSTPPFLIQIGAGRTVACWNPGG"
     gene            4909..5688
                     /locus_tag="P5541_07410"
     CDS             4909..5688
                     /locus_tag="P5541_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV71849.1"
                     /translation="MNVRELEAVKQWFMDYPERFRDSEGNLHRMHELKRDHSQRVAGY
                     CRFIAFEIGWSPREINTAEAIGWLHDVGRFPQWKEFGTFYDPASIDHGAKGREVLEKE
                     EILSCLPPEERTAILDSVILHNRREIPEETAPSTMPYARMIRDADKLDIFDLVYGHLS
                     AGTIRKLIPGISGEKAASPAIIEEITRDGRASYQNVRTIPDFILTQVSWVSNINYAPT
                     FRCIAEHKLIERLRSFLPAHHPGIEGIMESAIRRVARGCAG"
     gene            complement(5657..7846)
                     /locus_tag="P5541_07415"
     CDS             complement(5657..7846)
                     /locus_tag="P5541_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV71850.1"
                     /translation="MKKTDISFQGKEFYETLFEESPVGIVIQGIDGRVERANARFCKM
                     FGYSIEEVTGARLDDLVAFEPDLACSAAKLSTDVMRGRELALETERMRKDGSRFPVII
                     RGVPIVQGGKISAVYALYEDISETKEIENDLRRERAHFERIMLDSPDGIAVFDGEGTI
                     IRCNPAFEDLFGLESGTGVGSSLSEAVGSGGKIDQVKKNIEELRIRKRMDHESVRTRK
                     DGKDIFVAVRSAAISPDDNNEYMAVYRDISERKRSEEVLATERSYFENLFVNSPMAIA
                     LVDAKGIIQRVNSSFEDLFGYSDSECRGEYLDVLIAPEDSGDDAAQLTDQAAGGGSVR
                     VERVRCRKDRTCIDVQIIAVSFPGFGGERVVYAIYQDITERKIFEEHARFTSYHDSLT
                     GLFNQAFVEEEIQRLDTARQLPISVIMADLDNLKLVNDAFGHVEGDKLIIKTGNLLRS
                     CCRQEDIVARCGGDEFLILLPGTPMQDAGKICDRIREGCSSKWGESFIPLSIALGLAA
                     KTDVTEDFVQVIRKADEEMYLDKLMKSERSRQTIFERVETFLEGDHRRKSHIDRLANI
                     ASLFGEFMSLRKDEIDRLRLLARFHDVGLISVPADILFREGPLGPDEMEMIRKHPERG
                     YHIARAIPQITHVAEGILSHQEKFDGSGYPRGLAGESIPLAGRIMHLLCAYEAMTGWR
                     SYAPALSRREALEEIEEKTGSQFDPRLAGLFLKMMEEPSPRTPGLPF"
     gene            complement(7833..8303)
                     /locus_tag="P5541_07420"
     CDS             complement(7833..8303)
                     /locus_tag="P5541_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRV71851.1"
                     /translation="MKRNPTVILVRGALIAGVYAAVTLIFAPVSFGPVQVRVSEALTV
                     LPWIWIEAIPGLFVGCLIANLMGGFGVIDVIFGSAATLAAAFLTSRMPTRRMAPIPPV
                     VVNMVVVGAYLSVLLELPLISTMIYVGLGQAVACFGLGLPLLSFIEGRFPNEKN"
     gene            complement(8404..10032)
                     /locus_tag="P5541_07425"
     CDS             complement(8404..10032)
                     /locus_tag="P5541_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV71852.1"
                     /translation="MIENSRQGILELLVDLVSVPSISCSQAEVEMGRHIMSRLSELEY
                     FRDHPDSLRLEAVPDDPSGRGVVLALVEANPPTPDTVILAGHYDVVDTDDFGPLEDLA
                     FSPLEYTAALAEKGLEGEAGDDLRSGDYLFGRGVMDMKSGLATAMALLAEASAGGLFR
                     TNLLFIAVPDEEVGSAGMRAAVPMLARMQENRGLRYLGAIMTEPSSAGAPDAEDEVIS
                     LGSVGKLMPFFYCVGMGSHVGQYFDGFSASLLASHCSLLVEGNPALTDSAREETAPPP
                     SCLFMRDLRNGYSVTLPDRAALFFNMMTLRRTPSDVIKEMKAIAEKAFRNAIEQLEES
                     AAELGRKSPVITPAVVTFGEFASSTMESLGEDIKERIRKTVKAMDPLMDDRLRSLALI
                     TEMLAWTGHPAPMIITGFLPPYYPHRENAGESAGDLRARKVAQRVIETARKDYGVAMS
                     IREFFQGICDLSYLGFQGSAFDVFCMAANTPGWGSIYRIALKEMMGLDVPVLNLGPSG
                     KDPHKSTERLCLSYSLEVFPELLREAVKAFGETE"
     gene            10160..11347
                     /locus_tag="P5541_07430"
     CDS             10160..11347
                     /locus_tag="P5541_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71853.1"
                     /translation="MNFRIRRPSFNPRLAVLTATHFLNDIHSSFLPTFMPLIVERLGI
                     SYAQAGLLKSLSGIIHMVVQPLAGYVSDIFSRPYAIIAGPIITAIGASLFPMAPSYGL
                     ALVFVGLWGFGSAIFHPLGYGSVGYVGDPGKLAFFIAVFSVGGILGATLSPLYAIALA
                     KTVGTGPLLLAVTLVPVSAGAFLVMRYVPTLKAEGASEKPSPLGFARSFGKTFHVIFP
                     LWIICVCRDTAVEGIRFFLPLLIASRGGNIVNIGTVLFWISATGAISPMIGGRLADRF
                     GTRKVIEFTMALAPLFLVPAAMMNGIPSIALYMAGNALLQGILPVTSAASQEMAPGAR
                     STAASMVTGLSFGLAGLLTAPIGAMADSFGLTWVLVSMGVLPLLPMPVFWKWWKEKEG
                     DQE"
BASE COUNT         2632 a         3180 c         2895 g         2664 t
ORIGIN      
        1 atggcctgga gccttacgag ggatccggac agcctcttcg cttttttcca ttcgtccatg
       61 gatgtccctg gaggatacaa catccagggc ctccatgacg aggctcttga cagggttctg
      121 gagaaacttc gctgggcacc ggaccgtgaa actgccgata aggccgcaag aagggcacaa
      181 aaaatgctct cagagataac tccatggatc cctatctact cccgatacat ggtcacagcc
      241 gtaagagacg gctggaaagg gataatcgag acagaaagaa cgaccgcgga caacatctgg
      301 accctcatca acatggaacc gggaaacggg ggcgataggc ccatcatgtg gtgtctgcag
      361 gaggaaccaa ggtcgctcaa tcctttcagc acaagttctg tctacgactg gcaggtcctt
      421 ggcctggtct acgactctct tatcgcgatt gacccggaag atctcgagga catcccgtgg
      481 cttgccagat catgggaggt agagactacc ggttccgggt caacagcaaa gacccggtta
      541 accttcgcgc tcaggagcgg aatcaggtgg catgacggtg ttcttttcac ctccagagat
      601 gtaaggaaat ctctggaatt cctgaaatcc cgcgagatac caaggtattt cgacaacgtc
      661 agggacatag agagcataga aacacccgac gaaagaaccg tgatcgtcaa catgtccaac
      721 acctccttct ggcacctgca caacatcggt ggatcacccg ttttcccggc tcatataatt
      781 gaaaacgtca aagactggga gtcatggcag ccctcaaggg agaagcaccc atcggttgag
      841 gggcttacta tgctcattgg cacgggaccc tttgtttttc gggaatacag gcccggtgaa
      901 tatgtccact tcacccgttt cgatgacttc aggcttccct ggagagacta gaaattgaac
      961 agttactgga tccgaaggtt cgcgggagca ctggcagttc tcctgctgat tattacggca
     1021 aactttttcc tcttccgcgt tatgccaggc gatgccgtca gcacgatcgt ggacccccgt
     1081 ttttcccccg aggccaagga aaaaatgcgg catctctttg gcctcgatct gcccctccac
     1141 cagcaattct tcatatatgt caggaggatg ctctccttcg atttcgggat ctccttcctg
     1201 tcccagaggc cagtatggga cgagatcaaa agcaggatcc ccaataccct ggcgcttctc
     1261 ggaacctctc tccttctctc ttcggccatt ggtacatggc tgggagtcaa ggcagcaaga
     1321 aagagagacg gttttctcga gaaggccgtc atcttttcgg gatcgatatc cttctcgttc
     1381 ccctctttct ttgtccagtt ggtactgctg ctccttcttg cctacacctt ccctgttttt
     1441 cccctgagag gaacagtatc gatcccgatg cccgcaggaa ccttgccgcg tgttatcgat
     1501 tacattcatc acctgatact ccccgcaggg tctcttgtgc tccttggttt cgggtcatgg
     1561 gccatctacg tgagaaacct catggtcaag actctgggtg aagattttat tcttcttgcc
     1621 cgggcaagag gactctccga ggaccggatc atatggaacc atgctttccg ttcgaccctc
     1681 ccgccaatac tgacgatatt ccttctcgcc ctgcccggca tcgtttcggg ggcggtaatt
     1741 acagagacag ttttttctct gcatggggtc ggaaaattcc tcctcgactc gatacttgga
     1801 cacgactacc cctcggcagg tgctgcattc tacatactcg gtctactcac gatagcctca
     1861 aacctcgcgg ccgatgctgc ctgcaccctg gctgacccac ggataagggt cgggaaaagg
     1921 gctgaaaggt aaaatcatgc ccagatggag ttttgccgct ttttttatta ttgtcgcagc
     1981 ggccatagcg ggcccttccc tgcttcagac agatcctttt gaaagcgtcg caaggccctt
     2041 tgcccctccg gtctggactg gaggagagga gggacctgcg ctggctgtct cactgtcgcc
     2101 ttcgaagact ctcttcgaaa aaaaatggaa ctggtcccct ccggagaaca tctccatcag
     2161 gggaaccctt tcctttgagg aagatccttc ggcctcgctt gtctggaccg gcccaggggg
     2221 tgaacgtttc gtactggcag tatcaggcga tacccgggaa tggctgatcg atatcgatgc
     2281 cagggacgtt tccttcaagg cttcactggg tgtgcctcct ttcggaaatg ccacgtcagt
     2341 ccttttccgg aatcatgaaa acataagggt cgaaatagaa gggaccaccc cgtcggccgg
     2401 caagatagag atcaggactc ccggaggaaa atggggcctg atggggacag accagagagg
     2461 aagggatgtc ctgtccctct tcacggaagg cataaggata tctctcatag taggtttggc
     2521 tgccaccctg atatcctctg ccctgggact ccttgtcggc ctttccagcg gatatctcgg
     2581 tggaaagatc gattccttta tcatgagggg cgttgatgtt cttctttcaa taccggtcct
     2641 gcccatactt atggtcatcg tggggatctg gggaaagggc ctttggcagc tgatccttat
     2701 actcggactt ttctcatgga tgggaactgc caggacagtg agatctctcg ccctgactct
     2761 cagggaatcc tgttacgtcg agaatctccg ctcaataggg gcgagcccat ggtacatcct
     2821 ctggaggcat ctggtaccag aggctcttcc tgtccttctt gcagcggtca cactgggagt
     2881 gcccggagca attcttgctg aggcagggct ttcattcctg ggcctttcgg acccaagaat
     2941 aatctcctgg ggaaggatgc tccacgaagc ccacggcttc ggcgccttca cggcaggtgc
     3001 atggtggctt atccttcctc ccgggatcgg aatttcggta atctgtcttg tatttcttga
     3061 tatgggacga gccctcgagg agatagcgga cccgcggctc gtgaagggta acagaaaatg
     3121 atctgtatcc gcgacctttc ggttacttac aatacgaatg ccaggaaagt ccgcgcagtc
     3181 agggaagctt ctatcgatat ccaccactca gcggtaacag ccatagttgg agaatccggc
     3241 ggcggaaaga gcagtctgat ctactctata ctgggcctcc ttccccccga agcaacagcc
     3301 acagggaaaa ttctctttga gggttccgat attctttctg cacctgaagc taccagaagg
     3361 gatttcaggg ggataaaggt ggctcttgtg acccaggggg caatgaactc ccttactccc
     3421 gtgatctccg tgggagagca gatcgcggaa acccttcgaa caagactggg aacccgaaga
     3481 agggaagccg cagtaaaagc ctcggaactc cttgaaatgg tcggccttgg gcccagcttc
     3541 gcggtcagat ttccacacga actgtcggga ggggaaaaac agcgggtcgt aatagctctt
     3601 gctatatcct gctccccggt ttttttgctg gctgacgaac ccaccagcgc cctggacgtt
     3661 attacccagg ccgagataat aacattgctt ggcggaatgg tccgtgaaac cgggatggga
     3721 ctccttcttg taactcacga cctccccctt gcctcctcgg tatcggacag cataagcgta
     3781 atgcaccagg gaaagatagt agagagcggc ccggctgaaa agataatgac ttcgcccctc
     3841 caccctcaca caagggatct ggtctttgcg gcaaaccttg ctacggggag agggttccaa
     3901 tgagatcacc tctgttggta gcacaaaaga tcaccgtgag tttcggttcc gcaagaaacc
     3961 aggttcaggc ccttgccgga acggatctgg agatctttcc tggagaaagc ctcgcgatag
     4021 tgggtgaatc cggtagcggc aagaccacct tgctgagggc atgccttgga ctgacagcca
     4081 tatccggcgg atatgcctcc cttttcggaa aggacctttc actgtgcagc cggggggaac
     4141 tggttttcct ccgccggaga tccggatacg tgccgcagga ccctttcggt tgtgttccac
     4201 caactctgag cgcccttgat gcggtatccg aaccgatgct catagccgac tcacccggga
     4261 aaaaacgggc atcggaaaga gcaggagaac ttcttgtcga atgcggcctc gcagacaggg
     4321 aaatctggaa ggagcgagtc aggttttccc tctctggcgg ccagagacag cgcgtcagca
     4381 ttgcgagagc cctcgcgctt gacccggaac tgctcctggc tgatgaaccg gcaagcatgc
     4441 aggacgccgc cactcgaaaa ggaatcatgg aaatccttcg caagcgttgc gaaaagggca
     4501 tgggtcttgt aatggcgacc catgatcttc tcctcgcgtc ggcttccgcc tctcgggtaa
     4561 tggtccttta caggggacac gtggtggaaa cgggcccatc caaagacctg ctttccacac
     4621 ccgctcaccc atacacaaga gccctgggag cagcacttcc gggaatgggc cattctccgc
     4681 aaatacttgt tcgtcgtact gattccctct ccgggaaaac cggctgcgtt ttttcggcga
     4741 gatgcccccg gagaacagaa aaatgctcca cccccccttt cctgatacag atcggagcag
     4801 ggagaaccgt cgcatgctgg aacccgggtg gttaaaaatt ctgaaagtgg tatcatttct
     4861 gtttgttgaa ccatagcaag gcaacttgca ccgaagggct gtgaaaaagt gaacgtaaga
     4921 gaacttgagg ctgtaaaaca gtggttcatg gattatcccg agaggtttcg ggacagtgaa
     4981 ggaaacctcc accggatgca cgaactgaaa agggatcaca gccagagggt tgcaggatac
     5041 tgccgcttca tagccttcga gatcggctgg tcacccaggg agataaatac ggctgaggcc
     5101 ataggatggc tgcatgacgt gggacgattc ccacaatgga aggaattcgg tacattctac
     5161 gacccggctt ctatcgatca cggggcaaaa ggacgcgaag tgctggaaaa ggaggagatc
     5221 ctttcctgtc ttcctccgga ggagagaacc gctatcctgg attcggtgat acttcacaac
     5281 aggagggaaa tacccgaaga aacggcccca tccaccatgc cttatgccag aatgatccgt
     5341 gatgcggaca aacttgatat attcgacctg gtttacggac atctctctgc cggaaccata
     5401 cgtaaactca tcccgggcat ctccggcgag aaagcggcct cccctgcaat tatcgaggag
     5461 ataaccaggg atggcagggc ttcataccag aacgtaagaa ccatcccgga ttttatcctg
     5521 acccaggttt catgggtatc gaacataaac tatgccccca cgttcaggtg catcgcggaa
     5581 cacaagctga tagagaggct ccggagtttt cttcctgccc accaccccgg catagagggg
     5641 ataatggaat cggcgatcag aagggtagcc cgggggtgcg cgggctaggc tcctccatca
     5701 tcttcaggaa aagccccgca agacgggggt cgaactggct tccggttttt tcttctatct
     5761 cttccagggc ctccctcctc gacagtgccg gagcatagga acgccagccg gtcattgcct
     5821 cgtatgcgca cagaaggtgc attatccttc cggcaagagg tatagattcc cctgcaagac
     5881 cgcggggata accgctgcca tcgaactttt cctgatgtga gagaataccc tccgccacgt
     5941 gagttatctg tggaattgcc ctcgcgatat ggtaacccct ctcgggatgt ttacggatca
     6001 tctccatctc atcagggccc agtggtccct ctctgaaaag gatatcagcc ggaacggaga
     6061 tgagccctac atcgtggaaa cgggcaagaa gccttagtct gtctatctcg tcctttcgca
     6121 aggacatgaa ctctccaaaa agggaggcaa tatttgccag tctgtcaata tgacttttcc
     6181 tcctgtggtc cccctccagg aaagtttcaa ccctttcgaa tatcgtctgc cgtgacctct
     6241 cgctcttcat aagtttgtca aggtacatct cttcgtcggc cttccgaatg acctgaacaa
     6301 aatcctcagt cacgtcagtc tttgccgcca gacccaaggc tatgctcagg gggataaagc
     6361 tctctcccca tttactgctg catccctccc ttatgcggtc acaaattttt cctgcatcct
     6421 gcatgggtgt ccctgggaga agtatcagga actcatcccc tccgcacctt gccacgatat
     6481 cttcctgtcg gcagcacgac ctgagcaggt tgcccgtttt tattatcagt ttgtctccct
     6541 caacgtgtcc aaaggcgtcg ttgactagtt tcagattgtc aaggtctgcc atgatcacgc
     6601 tgatcggcaa ttgcctggct gtgtcaagac gctggatctc ctcctccacg aaggcctggt
     6661 tgaacaggcc agtaaggctg tcatgataac tggtaaatcg ggcatgctcc tcgaatattt
     6721 tgcgctctgt aatatcctgg tagatggcgt agacaactct ttcccccccg aaaccgggga
     6781 atgaaacggc gattatctgg acatcgatac atgtccgatc cttcctgcat cttactctct
     6841 ccacccggac actgccccca ccggctgcct ggtcagtcaa ctgggcggcg tcatccccgg
     6901 agtcttccgg ggctataaga acatcaaggt attcccccct gcactcactg tcgctatagc
     6961 cgaaaagatc ttcgaaagat gagttgaccc tctgtatgat tcctttggca tcgacaaggg
     7021 ctatggccat agggctgttc acgaaaagat tctcgaagta actcctttcg gtggccagaa
     7081 cctcctcgga ccttttcctt tcagaaatgt ccctgtaaac agccatatat tcattattat
     7141 cgtcaggaga aatagccgcg gaccggaccg ctacaaagat atccttaccg tctttcctgg
     7201 tcctgacgga ctcatgatcc attcgctttc taatcctcag ttcttcgata ttctttttca
     7261 cctggtctat tttgcctcca gatccgacgg cttcggacag ggaagatccc actcccgtac
     7321 cagattcaag gccgaacaga tcctcaaagg caggattgca gcggattatc gttccctccc
     7381 catcaaatac agcgatacca tctggggaat caagcattat tctttcgaaa tgcgcccttt
     7441 cgcgacggag atcattctcg atctcttttg tttctgaaat atcctcgtaa agggcataaa
     7501 ctgctgatat ctttccccct tgaactatgg gaacccccct gatgatgacg gggaagcggg
     7561 aaccatcctt acgcatcctt tcggtctcaa gggccagttc cctgcctctc atgacatccg
     7621 tcgataattt ggccgcagag caggccagat ccggttcgaa agccaccagg tcgtcaagtc
     7681 gagctcctgt cacttcctcg atggagtaac cgaacatctt gcagaacctg gcgttggccc
     7741 tttcgacccg gccatcgatt ccctggatca ctatcccgac gggagattcc tcgaacaggg
     7801 tctcgtagaa ttcttttccc tggaatgaga tgtcagtttt tttcattggg aaatctccct
     7861 tcgatgaatg aaagaagcgg cagcccgagg ccgaagcagg ccaccgcctg acccaaccct
     7921 acgtatatca tggtggagat aaggggcaac tccagaagca cagaaaggta ggctccaacc
     7981 acgaccatgt tgaccacgac cggggggatc ggcgccattc gcctggtcgg cattctggag
     8041 gtaagaaagg ccgctgcaag ggtggcagcg cttccgaaaa tcacatcgat aactccgaaa
     8101 ccacccatca gattggcgat aagacagccc acgaagagtc ccgggatagc ctctatccag
     8161 atccagggaa gaacggtaag tgcctccgat accctcacct ggaccggccc gaaagaaacc
     8221 ggtgcgaaga tcaacgttac cgcagcatat acacccgcga tcagggctcc tctcacaagg
     8281 atcacagtag gatttctttt catttgactc tcctggttgg ttccttttac ggaaatattg
     8341 ttataaacac tcaatgaata tctgttagta taagattaaa gacgtgacga aacaagtagc
     8401 cccctattcg gtttctccga aagccttaac tgcctccctc agcagttctg gaaatacctc
     8461 cagtgaatag gatagacaaa gcctctcggt cgatttgtgt ggatccttgc cagagggacc
     8521 caggttaaga acgggaacat caaggcccat catctccttc agggctatcc tgtagatgct
     8581 tccccatccc ggggtattcg cggccatgca gaacacatca aaggcacttc cctggaaccc
     8641 cagataactc agatcacaaa ttccctgaaa aaactccctg atcgacatgg caaccccgta
     8701 atccttcctt gcggtctcaa taaccctttg tgcgaccttt ctcgctctca ggtcaccggc
     8761 cgattcacct gcattttccc tgtgagggta atagggcggc agaaatccgg tgattatcat
     8821 cggagcggga tgacctgtcc aggcaagcat ttccgtgatc aaagcaagag acctcaacct
     8881 gtcatccatc aagggatcca ttgctttaac ggtctttcgg atcctctctt tgatatcctc
     8941 acctagagac tccatcgtcg aggaagcaaa ttcaccgaag gtaaccacag cgggagtaat
     9001 taccgggctc tttctgccaa gttccgctgc cgactcttct agctgttcga tggcattcct
     9061 gaaagctttt tcggcaatgg ccttcatctc ctttatcaca tccgaaggag ttcttcttaa
     9121 ggtcatcatg ttgaagaaaa gggctgccct gtccggaagt gtcacggagt atccgttccg
     9181 aaggtctctc atgaaaaggc aggaaggcgg cggggccgtc tcctccctgg cactgtccgt
     9241 cagggcgggg ttcccctcga caagaagcga acagtgagag gcaaggagag aagcactgaa
     9301 accgtcgaaa tattgcccga catgacttcc cataccaacg cagtagaaaa aaggcatcag
     9361 tttgccgacg gatcccaggg aaattacctc atcttcagca tccggtgcac cggcgctgga
     9421 gggttccgtc attatggcac ccaggtaacg caaccccctg ttttcctgca ttctggcaag
     9481 catcggcaca gccgccctca ttccggccga cccaacctcc tcgtcaggaa cggcaataaa
     9541 aagcaggttg gttctgaaaa gacctccggc cgaggcttcg gcaaggagag ccatcgccgt
     9601 tgccagaccg cttttcatgt ccattacccc acgaccaaag aggtagtctc cggatcgaag
     9661 atcgtctcct gcctctcctt caagcccttt ttcggcaagg gccgctgtgt attccagggg
     9721 agaaaaggca agatcttcaa ggggaccgaa atcgtcggtg tcgacgacat cataatgccc
     9781 ggcaaggatc acagtgtccg gtgtcggcgg gtttgcctcg acaagggcaa gaacaacgcc
     9841 ccttcctgat gggtcatcgg gaaccgcttc aaggcggaga ctgtctggat ggtccctgaa
     9901 atattccagt tcggaaagcc ttgacataat atgcctgccc atttcaacct cggcctgtga
     9961 acaggagata ctgggaaccg atacaaggtc cacaagaagt tcaaggatcc cctgtctgct
    10021 gttttcgatc actaagagtc actcccttgc aagtcaggat cagtctaaaa cagacaatga
    10081 ataatcatac cgcatattta tgcatgcaat gatacaatga cggtcgaagc aatatataca
    10141 tagaaaagga gcttttataa tgaatttccg catcagacgc ccctccttca acccaaggct
    10201 ggcggttctc acggcaacac atttcctgaa cgacattcac tcctccttcc tgccgacatt
    10261 catgcccctt attgtcgaac ggctcggaat atcctacgcc caggcggggc ttctgaagtc
    10321 cctttcgggg atcatacaca tggttgtgca gcccctggca ggatatgttt cggatatttt
    10381 ttccaggccc tacgccataa tcgccggccc cataataacc gccattggag cctctctctt
    10441 ccccatggct ccaagctatg gcctggccct tgttttcgtg ggcctgtggg gcttcggaag
    10501 cgcgatcttc cacccccttg gctacggaag tgtcggctat gtgggagacc ctggcaaact
    10561 ggcattcttc atcgccgtat tctcggtggg cgggatcctg ggcgccactt taagccctct
    10621 ttacgctata gcccttgcca aaaccgtggg gacaggacca ttactgcttg cggtgaccct
    10681 tgtccctgta tcagccggcg cttttctggt aatgcgttac gtccccacac tgaaagctga
    10741 gggagcatca gaaaaaccct ctcctttggg tttcgccagg tcattcggga aaactttcca
    10801 tgtaatcttt ccgttatgga tcatctgcgt ctgccgcgat acggctgtgg aaggaatacg
    10861 cttctttctg cctcttctga tagcgtcgag ggggggaaac atcgtcaata tcgggacggt
    10921 gctcttctgg ataagcgcga caggagcaat atctcccatg ataggagggc gtctcgcgga
    10981 ccgcttcgga acaagaaagg ttattgagtt cactatggct ttagccccgt tgtttctagt
    11041 ccctgcggca atgatgaacg gaataccctc gatagcgctc tacatggcgg gtaacgccct
    11101 tctccagggc atcttacccg tgaccagcgc cgcgtcgcag gagatggcgc cgggggcgag
    11161 gagtactgcc gcttcaatgg taacaggact ctcttttgga ctagcggggc ttctgaccgc
    11221 tcccatcgga gctatggctg actctttcgg gctgacatgg gttcttgtct ccatgggtgt
    11281 tcttccactg ctcccgatgc cggtcttctg gaaatggtgg aaagaaaaag aaggggatca
    11341 ggaatagcgg aaaagcaaga gcgtgaggcg t
//
LOCUS       DAOTIQ010000058        11051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_7027_length_11051_cov_8.145689, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000058 DAOTIQ010000000
VERSION     DAOTIQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7027_length_11051_cov_8.145689"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1043
                     /locus_tag="P5541_07435"
     CDS             228..1043
                     /locus_tag="P5541_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV71854.1"
                     /translation="MKRFGVFFAVSALLFGMFAGSAMAGQIQVNGSTTVLPIAQAAAE
                     AYMEAHPDVNISISGGGSGNGIKAIIDGTTDIANASRFIKQSEVEAAVKNGSYPVPFA
                     VAVDAIIPVVHPSNPVKDLSIDQLRDIYMGKITNWKEVGGPDKKIAAVTRDTSSGTFE
                     VWEEKVMNKEKIMPAAQVVASNGAMVQSVAGNPAAVGYIGIGYLDKSTKALLVNGIEG
                     TSKNALNGSFPVSRYLYMFTKGWPTGEVMDFMNFILSDAGQKIVAESGFVSLR"
     gene            1137..2018
                     /gene="pstC"
                     /locus_tag="P5541_07440"
     CDS             1137..2018
                     /gene="pstC"
                     /locus_tag="P5541_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRV71855.1"
                     /translation="MTTSLKEDRIPRLVIMAVASLGVAIMLFILYSLVRDSIPILAHV
                     TLSDLLLGIHWYPTYDPPEFGMLPLLVGSLSVTFAASLLSLPVSLALAVFLSEVCPAT
                     LREILKPVIEILGFFPSVVLGFIGMVVIAPWMVTNFDIITGLNLLNASVLMGIMIIPI
                     VTSLAEDSLNSVPRDIRDASFALGATRIETVLRSVIPAALPGLMTAALLGVMRAIGET
                     MVVLMAAGGAAIIPRSLMDPVRPLTSAIAAEMGETPFRSDHYYALFFAGLLLLLLTLA
                     LNMTALWIESRGKRWRS"
     gene            2015..2878
                     /gene="pstA"
                     /locus_tag="P5541_07445"
     CDS             2015..2878
                     /gene="pstA"
                     /locus_tag="P5541_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRV71856.1"
                     /translation="MSTGRMAKDRAATILLWSVMILVCAILALILAFLVSKGWSAISW
                     EFLTKKPRSMMTAGGISTPIVGTLQLVLVAMAFSFPVGVFTAVYLVEYARDDWFTRML
                     RLAVRSLAGVPSVVFGLFGLAFFVILMNFGSSLLSAGLTLGCLALPVIVGASESALLA
                     VPRDYRDASYALGATQWQTIRKVVLPTAFPSIITGAILSVGRVAGETAPIIFTGAAFY
                     APRFAKSLFDQVMALPYHILILATAGTNIEKTRPIQYGTVLVLLFFVLGMTMTGVLWR
                     ARLRIRQRAQR"
     gene            2900..3658
                     /gene="pstB"
                     /locus_tag="P5541_07450"
     CDS             2900..3658
                     /gene="pstB"
                     /locus_tag="P5541_07450"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRV71857.1"
                     /translation="MGNDKFVIQGLDLFYGTNQALKDINLKIPERAVTALIGPSGCGK
                     STFLRCLNRMNDFITTCRITGTVELDGGNIYDPETDLILLRRRIGMVFQRPNPFPMSI
                     FDNVAYGPRLHGDRNKGSLRDIVEESLQVSALWNEVRDKLHTPALALSGGQQQRLCIA
                     RAVAVQPEVLLMDEPTSALDPMATARIEELVRELRTRYTVVIVTHNMQQAARISDTTD
                     FFLMGEMIESGPTQMMFTSPKDKRTEDYITGRFG"
     gene            3682..4380
                     /gene="phoU"
                     /locus_tag="P5541_07455"
     CDS             3682..4380
                     /gene="phoU"
                     /locus_tag="P5541_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRV71858.1"
                     /translation="MTSLTGRKEFDESLLTIEQNLLKLGSLAEDAISNSIWALQKQDE
                     ALAMKVIQGDDVLDAITQEINEQALMMIARYQPVALDLRTISSILHMATDLERIGDLG
                     SGVAKVARKIFHEPLIKPLIDIPRMGERIREMTDEALKAFMNRDSALAREVCAMDDEV
                     DDLDRQIFRELLMLMMQNPRNIEQATHLILISRNLERAGDHVTNLCEHIVYMTTGSFV
                     KAGDFRRPHEGKTD"
     gene            4384..5094
                     /locus_tag="P5541_07460"
     CDS             4384..5094
                     /locus_tag="P5541_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV71859.1"
                     /translation="MPERILVVEDEKTIAELVAEALKRRGYRVELAHDGDSGLLAAET
                     SLPDLVILDVMLPGMDGWEVCRRIRENPSTRNTPVLMLTARREERDLLAGFDVGADDY
                     MKKPFSVNELAARVRSLLRRAKGGAEEGSITSAGPLIVDNDREEAFLDGAPLDLTATE
                     FRILEMLAANVGRVVSRDALLARVWGYAAADTRTVDMHVFRLRRKIEKDPENPRLIHT
                     VRGRGFRLQEEGVADENS"
     gene            5081..6832
                     /locus_tag="P5541_07465"
     CDS             5081..6832
                     /locus_tag="P5541_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV71860.1"
                     /translation="MRTLKGRMTDLIATVLVLALVSGWFLVGNSVEERMISQTENDLL
                     SRVTILAGVLQDSGVKGVESNVTRWAGDARTRVTLITMDGKVIFDSEADPATMDSHAG
                     RPEVREALLSGTGINTRYSRSIESDQIYVARLAARPDGEALVVRISLSLSEVESAVAS
                     ARKRILVSLAFAGIASLIAGLFFIRQVTRPLEELTISALETKNGAKLRFPSSGSLEIQ
                     RLATALGGMSERLDEVLEDLRREKAYLRSLLEALPTGVLVVDMQKKIRYANAALGKLL
                     RDIPDRMDGCFYTGAIRKPELIDLIDSAFGGEDRRESFVVRDRTETFLEAQSVNIGQG
                     VLVVLNDFTERHRLEETRRTFVADAGHELQTPLTSIRAAAELLLDEGGPNPEATRELA
                     EKIVLQQERMTTLVDDLLLLSRLESVVTPEKGELVELDRVMEEAVSYHRELPQASSIR
                     WETTINGPAPFTGRPDELDRAFGNLLDNAVKYTRKRFGDSPGGVIKVSLEHHGDLWKI
                     MIEDNGTGIDESVREAVFERFQRGEKSRSRSAGYSGGYGLGLAIVKRIVESHGGKIEA
                     LKSDEGALLAVTLPCNV"
     gene            6949..7686
                     /locus_tag="P5541_07470"
     CDS             6949..7686
                     /locus_tag="P5541_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRV71861.1"
                     /translation="MIRKILRDSVLSLLIGCTVSFLVYTTAGDLLAESPADAVSRWSR
                     ARVVSKRVLALDATVAAALGEMDLEPKDIVEWHMDDPESNLRKLWQMKPADQKNASVI
                     ALYIIHQNRNIDAETVWRQAVSFVHYSKKYDVPLTLAVAVANTESHFNPDARSSYGAA
                     GVMQVVWRIHSDLLKVHGGIRSERDLHDPEKGIAAGTLLLSRYLQAYGSTQGALGRYY
                     GGPVENYWPKVSRNINRVVSYGLEPGI"
     gene            complement(7744..8814)
                     /gene="corA"
                     /locus_tag="P5541_07475"
     CDS             complement(7744..8814)
                     /gene="corA"
                     /locus_tag="P5541_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRV71862.1"
                     /translation="MPKNRRKRILRHQKPGLPPGELVHVGDRKTEDVAISLFHYSASS
                     LEEKQSCLLNELPSPPPPGEVAWLNVIGIHDTEALATIGEKYGIHPLVLEDICNTGQR
                     AKAEDYGDYQYVVVKMVSWNETAMEIEVEQVSLVLFGTVLISFQELPGDEFGPVRQRL
                     RLGKGRLRKQGADYLAYSLLDAIVDGYFFLIEKIGDNVELVETELLERPDSGTAGHIH
                     ELKREMIFLRKAAWPLREAVSALERSDRFSGSGTAIYMRDLYDHVIQVIDGVETIRDI
                     LAGMLDIYLSSVSNRMNEIMKVLTIIATIFIPLTFIAGVYGMNFHYMPELGWKWAYPV
                     LLGVMGVTAVGMLRFFRRKGWI"
     gene            complement(8842..9627)
                     /gene="xth"
                     /locus_tag="P5541_07480"
     CDS             complement(8842..9627)
                     /gene="xth"
                     /locus_tag="P5541_07480"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRV71863.1"
                     /translation="MGIFSIATFNVNSVRSRLPILDRWLDDNRVDCLCLQETKVEDGS
                     FPAGFFEEKGYTVQYRGEKSYNGVAIASLVPPDEYGFGLGDGEDPSGETRIAWCRFGE
                     LSVVNAYVPQGKSIDHPDYPMKLAFFKRLQELLSGLGAPDKPLLLLGDLNVAPSDIDV
                     TNPGNKRDHVCFHSGVREAFHELCSVGLVDIFRKHRPGEGEFTFWDYRVKGALERNIG
                     WRIDHILGTRVQEGNSLDCFCDREPRSWEKPSDHSVVVARFSK"
     gene            9708..10211
                     /locus_tag="P5541_07485"
     CDS             9708..10211
                     /locus_tag="P5541_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRV71864.1"
                     /translation="MDPDRKHRHWFMVLFAILAAGTTGIRFFTGSSWIDSLFYTVITL
                     STVGYGAPPGLPPGGKVFVILLILIGIGTAGLAIGEITRYFVVERMLSVMGKRRDKRV
                     KDLRNHWILAGLGRVGMEVARHLENDNIPFLALEIADRKVASAREKDGSSSRATSARR
                     PCWRKRE"
     gene            10190..10723
                     /locus_tag="P5541_07490"
     CDS             10190..10723
                     /locus_tag="P5541_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRV71865.1"
                     /translation="MLEEAGITKARGLILTLSDDSDNVYATLTAKALNPDMRVIARAN
                     DPQSTKLLLRAGAERAMNLADAEAAAIARASINPAVADFLEMVNISRDLGLDFSAATL
                     HPESPVAGKTLAEAPLRFEYNAMVIAIKKEKGSIIYNPPGNEILEGGDELILFAEMDK
                     MEELRSLLCRPGGTGPE"
BASE COUNT         2527 a         3087 c         2987 g         2450 t
ORIGIN      
        1 gtctcctgtc tcctgtctcc tgtctcctgt ctcctgtctc ccgtctcccg tctcctgtct
       61 cctttctcct tgctgttctg tcatgtcttt atcagtttac ataagtgttt atgtttctgt
      121 tacaataccg ttacaaagcc gatgaaaaga cgttacaaca cttccttata ctttctccca
      181 gaaaggccgg caaaaggccg gtaccctttt caaggaggaa aataaaaatg aagcgtttcg
      241 gagtgttttt tgcggtttct gctcttcttt tcggcatgtt cgcagggtcg gccatggctg
      301 gccagatcca ggtaaacggc tccaccacgg ttcttcctat cgcccaggcg gcagctgagg
      361 cctacatgga agcccacccc gatgtcaaca tctccatctc gggcggcggc agcggcaacg
      421 gcatcaaggc gatcatcgac ggcaccaccg acattgcaaa cgcctccagg ttcatcaagc
      481 agagcgaagt tgaagcagcc gtcaagaacg gctcctatcc ggttcccttc gcggtagccg
      541 tcgacgccat cataccggtg gtacatccct ccaaccccgt taaagacctt tccatcgacc
      601 agcttcgcga catctacatg ggcaagatca ccaactggaa agaggtcggt ggacccgaca
      661 agaaaatagc cgctgtgacc cgcgacacca gttccggcac ctttgaggtc tgggaagaga
      721 aggtcatgaa caaggaaaag atcatgcccg ccgcccaggt cgtggcatcc aacggagcca
      781 tggtgcagtc cgtggccggc aacccggcag ctgtaggcta catcggcatc ggctacctcg
      841 acaagagcac aaaggctctc cttgtaaacg gcatagaggg gacctcaaag aacgcgctca
      901 acggttcttt ccctgtttca cgttaccttt acatgttcac aaagggctgg ccaactggcg
      961 aagtcatgga tttcatgaac ttcatcctca gtgatgcagg ccagaagata gtagcagagt
     1021 ccggtttcgt ctcccttcgc tagaccctga tacataatta ctgtttcccg gcccgaaccc
     1081 atgctgacat aatgggttcg ggcccttatt cacttcacaa aaaggagggt tgaaccgtga
     1141 cgacatctct taaagaggac aggatacccc gactcgttat tatggccgtc gcctctctcg
     1201 gcgtggcgat aatgcttttt attctttact ccctggtccg tgacagcatt cccattcttg
     1261 cccatgtcac cctgtccgac cttcttctcg ggattcactg gtaccccacc tacgacccgc
     1321 cggagttcgg gatgcttccc ctgctggtgg gatcactttc tgttacattc gccgcctctc
     1381 ttctgtctct gccagtcagc cttgccctgg ctgtctttct ttcggaagtc tgcccggcga
     1441 cccttaggga aattctcaag ccggttatag aaatccttgg gtttttcccc tcggtggtcc
     1501 tcggttttat cggtatggtg gtcatcgccc catggatggt tacaaatttc gacatcatta
     1561 cggggcttaa ccttctaaac gcttctgtcc ttatggggat aatgatcatc cccatagtaa
     1621 cgtcccttgc agaggacagt ctcaactccg tccccaggga cattcgggac gcatccttcg
     1681 ccctcggcgc cacaaggatc gagacagtcc ttcgcagcgt gatccccgct gccctgcccg
     1741 gtcttatgac tgccgcactt ctgggagtaa tgagagcgat cggagagaca atggttgtcc
     1801 ttatggcagc agggggggca gcaattattc cgcgttccct gatggaccct gttcgcccgc
     1861 tgacctcggc aatagccgca gagatggggg agaccccttt ccgctcagac cactactacg
     1921 ctctcttttt tgccgggctg ctgctgcttc tgctgaccct cgccctcaac atgacggccc
     1981 tctggatcga gagccgcgga aagaggtggc gctcatgagc accggacgca tggcaaagga
     2041 cagggccgcg acaatactgc tctggtcggt aatgatcctt gtttgcgcga tccttgccct
     2101 tatcctggcc tttctggtct cgaaggggtg gagcgcgatc agttgggaat ttttgacgaa
     2161 aaaaccaaga agcatgatga ccgccggtgg tatctcgaca ccgatagtcg gcacccttca
     2221 actggtgctg gtcgcgatgg cgttctcctt ccccgtcggt gttttcacgg ctgtttatct
     2281 ggtggagtat gctcgtgacg actggtttac aaggatgcta cggctggccg ttcgaagcct
     2341 cgcgggggtc ccctcggtgg tattcggtct tttcggcctc gccttctttg tcatcctgat
     2401 gaacttcggt tcgtcccttc tctcggcagg acttacattg gggtgccttg ctcttcctgt
     2461 aatagtgggc gcttcagagt cggcgttgct ggccgtgccc agggactacc gggacgcctc
     2521 ctatgccctt ggggccactc agtggcagac catccgcaag gtggtgcttc ccactgcctt
     2581 tccctcgata ataacgggag ccatcctgag cgttgggagg gtggcagggg agacggcccc
     2641 gatcatattc accggagccg cgttctatgc tccccgtttc gccaaaagcc tcttcgacca
     2701 ggtaatggct cttccgtatc acatactcat tcttgcgaca gcgggaacca acatcgagaa
     2761 gaccaggccg atccagtacg ggacggtcct ggtcctgctt tttttcgtgc tcggaatgac
     2821 aatgacgggc gtgctctggc gggcaaggct gcggatccgc cagcgagccc agcgttaggg
     2881 acggaaggtg gttcttccaa tgggaaatga caaattcgta attcagggcc tggatctttt
     2941 ttatggaacc aatcaggccc tcaaggacat aaacctgaaa attccggaga gggccgttac
     3001 ggccctgatc ggcccctcag ggtgtggaaa gagcaccttc ctgcggtgtc ttaaccggat
     3061 gaatgacttt atcaccacct gccgaattac agggacagtg gaactggacg gaggaaacat
     3121 ctacgacccg gagaccgacc tgatcctgct gaggcgtcgt atcggaatgg tcttccagag
     3181 acccaacccc tttcccatgt ccatattcga caacgtggcc tacggcccca ggcttcacgg
     3241 cgacaggaac aagggctccc ttcgggatat cgtcgaagag agtctccagg tatcggccct
     3301 ctggaacgag gtgagggaca aactgcacac ccccgccctt gccctctcgg gggggcagca
     3361 gcagagacta tgcatcgccc gcgcggtggc agtccagccg gaggtccttc ttatggacga
     3421 gccgacctcg gccctcgacc ccatggctac agcccgcata gaggagctgg tccgtgaatt
     3481 gcgaaccagg tacaccgtcg ttatagtgac ccacaacatg cagcaggccg ccagaatatc
     3541 tgacaccacc gacttcttcc tgatgggaga gatgatcgaa tcggggccga cgcagatgat
     3601 gttcacttcc cccaaagaca aaagaacaga ggattatatt accggccggt tcggataggc
     3661 tggcagagga gtgatcgggc aatgacaagt cttacaggac gcaaagagtt cgacgagagt
     3721 cttcttacta tcgagcaaaa cctgcttaaa ctcggatctc tggccgaaga tgccataagc
     3781 aattcaatat gggcccttca gaaacaggac gaagctcttg ccatgaaggt aatacaggga
     3841 gacgatgttc tggacgcgat cacccaggag ataaacgaac aggccctcat gatgatcgca
     3901 cgataccagc ctgtggcgct ggatcttcgg accatatctt ccatcctcca catggcgact
     3961 gacctggaga ggattgggga cctgggcagc ggggtagcaa aggtggcaag aaagatcttc
     4021 cacgaacctc tgataaaacc tcttatcgat attcccagga tgggcgagag gatcagggag
     4081 atgaccgatg aggcgctgaa ggccttcatg aacagggact cagcccttgc gcgcgaggta
     4141 tgtgcaatgg acgacgaggt ggacgacctg gacaggcaga tcttccggga gctgctgatg
     4201 ctgatgatgc agaacccgcg aaatatcgaa caggcaacac acctgatcct gatctccagg
     4261 aaccttgagc gggccgggga ccacgtaact aacctttgcg aacacatcgt ctacatgaca
     4321 accggttctt tcgtcaaagc gggagacttc cgaaggcccc atgaagggaa gactgattag
     4381 aaaatgcccg agaggatcct tgtcgtcgag gatgaaaaaa ccatagcgga gctggtggct
     4441 gaggccctca agaggcgggg ttaccgcgtc gagttggccc acgacggtga cagtggtctg
     4501 cttgcggcgg agacatccct gcccgatctg gtgattcttg acgtaatgct ccctggaatg
     4561 gacggatggg aggtctgcag gaggatcagg gaaaacccct ccaccagaaa cacgcctgtc
     4621 ctcatgctga cggcaaggag ggaagagagg gatcttctgg caggtttcga tgtgggagcc
     4681 gacgactaca tgaagaaacc cttctcggta aacgaactcg ccgcaagggt cagatctctt
     4741 cttcggcggg caaaaggggg cgccgaagag ggctccatta caagcgcagg tcccctgata
     4801 gtagataatg acagggaaga agcctttctc gacggggcac ccctggacct taccgctacg
     4861 gagttccgta tccttgagat gctcgcggcc aacgtcggac gtgtcgtctc cagggatgct
     4921 ctccttgccc gggtctgggg atacgccgcg gcagatacga gaaccgtgga catgcatgtt
     4981 ttcagactga gaagaaaaat agaaaaggat cccgaaaacc cgagacttat ccacacggtc
     5041 cggggacggg ggttcaggct tcaggaagag ggtgtcgccg atgagaactc ttaaaggccg
     5101 tatgaccgac ctcatagcta ccgtgctggt ccttgccctg gtctccggct ggtttcttgt
     5161 cgggaacagc gtggaagaga gaatgatctc ccagacggaa aatgatcttc tttccagagt
     5221 gaccatcctg gctggagtgc tccaggactc tggggtcaag ggggttgaat ccaacgttac
     5281 gaggtgggcc ggcgatgccc ggacacgggt aacactgatc accatggacg gtaaggtgat
     5341 cttcgacagc gaggcagacc ccgcaactat ggacagtcat gccgggagac ccgaagtaag
     5401 agaggctctt ctctcgggca ccggtattaa caccaggtac agcagatcca tcgagagcga
     5461 ccagatatac gtcgctcgcc ttgctgccag gccagacggc gaggcccttg ttgtaaggat
     5521 ctcactctcc ctctccgagg tcgagagtgc cgttgcctcc gcaagaaaaa ggatcctcgt
     5581 ctctctggca tttgccggca tagcgtcact cattgcgggg ctctttttca ttcgacaggt
     5641 tacccgcccc cttgaagaac tgactatttc cgcactcgag acaaaaaacg gcgccaaact
     5701 gcgtttcccc agctctggca gccttgaaat acagcgcctt gccacagcac tggggggaat
     5761 gtccgagagg ctcgacgagg tccttgagga tcttcgccgg gaaaaagcat atctgagatc
     5821 tctacttgaa gctctcccca cgggtgtgct tgtcgtcgac atgcagaaga aaatacgcta
     5881 cgcgaacgcc gcccttggca aactgctcag ggatatccct gacaggatgg acgggtgctt
     5941 ctatacaggg gctatcagga aacctgaact gatcgacctg atcgacagcg ccttcggcgg
     6001 agaggatcgt cgcgaatctt tcgttgtccg ggatcgaacc gagacattcc tggaggccca
     6061 gagcgtcaac ataggtcagg gtgttctggt ggtactcaat gactttaccg agagacacag
     6121 gttggaagaa acaaggagaa ctttcgtcgc cgacgcgggg cacgaactcc aaacccctct
     6181 cacctccata agggcggcag ccgaactgct tctcgacgaa ggcggaccga accctgaagc
     6241 aaccagggaa ctggctgaaa aaatagtgct tcagcaagag aggatgacaa ccctggtgga
     6301 cgatcttctt ctgctttccc ggctcgaatc ggtggttaca cccgaaaagg gagagctcgt
     6361 cgaacttgac agggtcatgg aagaggctgt ctcctaccac agagagctcc cccaggcttc
     6421 ttcaatacgc tgggaaacaa ccataaatgg gcccgcacct tttacgggga ggcccgacga
     6481 actcgaccgg gccttcggga acctgctgga caacgcggtt aaatatacaa ggaagagatt
     6541 cggggattcc ccaggagggg tcatcaaggt ctctctcgag catcacgggg acctatggaa
     6601 aataatgata gaagacaacg gcaccgggat agatgaaagt gtcagggaag ccgttttcga
     6661 aaggttccag agaggcgaaa agagccgctc cagaagtgcc ggatactccg ggggatacgg
     6721 cctgggactt gccatcgtca aaaggatcgt cgaatcacat gggggaaaga tcgaggccct
     6781 caaaagtgat gagggagcac tcctggccgt tacattgccg tgcaatgttt aaaaaaacgt
     6841 aagattttac accagggttg ctttttttgc aagtcccggt ataataccct ggttggtata
     6901 tcgctaacaa atgtataaat tatgcaaaaa cccgaagggg gtcatctttt gatccgaaaa
     6961 atcctgaggg attccgtttt gtcgttgttg atcggttgta ccgtctcttt cctggtctac
     7021 acgactgcag gcgatttgct ggcggagtcc ccggcggatg ccgtctccag atggagtcgg
     7081 gcccgtgtcg tctcgaagcg cgttctggct cttgacgcca ccgtcgccgc tgctcttggt
     7141 gaaatggacc ttgaaccgaa agacatcgtt gaatggcaca tggacgatcc ggaatcgaat
     7201 ctcagaaagt tgtggcagat gaagcctgcc gaccagaaga acgcttccgt gatcgcgctc
     7261 tacattatcc accagaatcg caacatcgat gcggagacgg tctggagaca ggctgtttct
     7321 ttcgtacact acagcaaaaa gtacgatgtt cctctcactc ttgccgttgc ggtcgccaac
     7381 acggagagcc atttcaatcc tgatgcgaga agttcatacg gcgctgccgg tgtcatgcag
     7441 gtggtatgga gaattcactc cgatctcctt aaggtccacg gagggatccg cagcgaacgg
     7501 gatcttcacg atccggaaaa aggcatcgct gcaggcacac tgcttctttc caggtacctc
     7561 caggcatatg gaagcaccca aggagccctt ggaaggtatt acggcggtcc tgtggaaaat
     7621 tattggccaa aggtttccag aaatatcaat cgtgtagtaa gttacgggct agaacccggt
     7681 atttagaaaa ctttgtccac aggcttatcc acattttgtg gataagcctt tttttgcgaa
     7741 atcctagatc cagcccttcc ttctgaaaaa acgcagcatt ccaaccgccg ttactcccat
     7801 aacaccaaga aggacagggt aggcccattt ccaccccagc tccggcatgt agtgaaaatt
     7861 cataccgtaa acccccgcga tgaaggtcag tggaatgaaa atggtggcga ttatcgtaag
     7921 gaccttcatg atctcgttca tcctgttgct gacacttgat aaataaatgt cgagcatccc
     7981 cgccagaata tcccggatag tctctacccc gtcaattacc tggatcacat gatcgtagag
     8041 atccctcatg tagatcgccg tgccgcttcc tgagaatctg tcgcttcttt caagggctga
     8101 cacggcttcc ctcaggggcc aggcggcctt cctcaggaaa atcatctccc tcttgagttc
     8161 gtggatatgg cccgcggtac ccgaatccgg ccgttccagg agttctgtct ctaccagttc
     8221 aacattgtct cctattttct cgatcagaaa gaagtagccg tcaacaatgg catcaagaag
     8281 cgagtaagcc aggtagtcgg ctccctgttt acgaaggcgc ccctttccca ggcgcagccg
     8341 ctgccttacg ggtccgaact cgtcgcccgg tagttcctgg aacgaaataa ggacggtgcc
     8401 gaaaagcacg aggcttacct gttcgacctc tatctccatg gccgtttcgt tccatgagac
     8461 catcttgacg accacgtact ggtaatcacc gtaatcctcc gccttggccc tctgccccgt
     8521 gttgcagatg tcctcgagaa ccagcgggtg gatcccgtac ttctccccga tcgtcgcgag
     8581 agcctcggtg tcatgaatcc cgatgacgtt caaccaggca acttcgcctg ggggaggtgg
     8641 cgagggaagt tcgttcagga ggcaggattg cttctcctcc agggaactgg ccgaataatg
     8701 gaacaatgat attgccacat cttcggtctt cctgtccccg acatgaacca gttcaccagg
     8761 gggaagtccc ggtttctggt gtctgaggat cctttttctc cggtttttcg gcatgcttta
     8821 cctcccggcc cagttctggg cctacttgct gaatcgggct actacaacgg agtggtccga
     8881 cggtttctcc caggagcgtg gttcacgatc gcagaaacag tccagagagt ttccctcctg
     8941 aacccgggtg ccgagaatat ggtctatcct ccagcctatg tttctctcaa gagctccctt
     9001 gaccctgtaa tcccagaagg taaattcacc ctcgccaggg cgatgttttc tgaatatatc
     9061 cacaagcccc acggaacaaa gttcatgaaa ggcctccctg acccccgagt ggaaacagac
     9121 atgatcccgc ttgttccccg ggttagtcac gtcaatatcg gaaggggcaa cattgaggtc
     9181 cccgagaaga agaaggggtt tgtcaggtgc gccaaggccg gaaagcaatt cctggagtct
     9241 cttgaaaaat gcaagtttca tggggtaatc agggtgatct atggatttcc cctgaggaac
     9301 gtaggcattc accaccgaca gttccccgaa ccgacaccag gcaatgcggg tctcgccgga
     9361 ggggtcttcc ccgtcgccaa gtccgaaacc gtattcatcg gggggtacaa gggaagcgat
     9421 ggccacaccg ttataggatt tttctcccct gtactgcacg gtatatccct tttcctcgaa
     9481 aaaccccgca gggaaagaac catcctcgac cttggtctcc tgtaggcaca ggcagtccac
     9541 cctgttatca tcaagccagc gatcaagtat cgggagacgg cttcttacgg aattgacatt
     9601 gaatgttgct atactgaaaa tacccatagg gctcacctcc atctaaaatg ccttttacta
     9661 ttctaccgct cctgaagccc tgaatgtcat tcgaaaggag agatcccgtg gatccggaca
     9721 gaaaacaccg tcactggttt atggtccttt tcgccattct cgcggctggt acaacaggaa
     9781 tccggttttt taccggatca tcgtggatcg attcgctctt ttacacggta ataacccttt
     9841 cgaccgtggg atacggggct cccccgggat tgcctcccgg agggaaggtt ttcgttatcc
     9901 ttctcattct tatcggcata gggacggcag gtctcgcaat aggagagata acgcgttact
     9961 tcgtggtgga gagaatgctc tctgtcatgg gcaaaaggag ggacaaacgg gtgaaagatc
    10021 ttcgaaacca ctggattctg gccggacttg gaagagtggg catggaggtc gcccgccatc
    10081 tcgaaaacga caacattccc tttctggcac ttgaaattgc cgacaggaag gtggcctcgg
    10141 cgcgcgaaaa ggatggatcg tccagcaggg cgacgtccgc caggaggccg tgctggagga
    10201 agcgggaata accaaagccc gcgggctgat acttacgctc tccgatgact ccgacaatgt
    10261 ttacgccacg ctgacggcga aagcactgaa ccccgacatg agagtcatcg cccgcgcaaa
    10321 cgatccccag tccaccaaac tgctcctgag ggccggcgcg gagagagcca tgaacctcgc
    10381 cgacgccgaa gcggcagcca tagcaagggc ctctataaac ccggcggtag ccgattttct
    10441 ggaaatggta aacatttcac gcgacctggg cctggatttc agcgccgcca cgttgcaccc
    10501 cgaaagcccc gtcgccggca agaccctggc ggaagccccc ttgcggttcg aatacaacgc
    10561 gatggtaata gccatcaaaa aggaaaaagg ttcgataatc tataaccccc cgggaaacga
    10621 gatccttgaa ggaggggacg aactgattct gtttgccgaa atggacaaaa tggaggaact
    10681 cagatctctt ctctgtcgcc ccggagggac gggaccggag taaagtcttt ccgaaaggcg
    10741 actcgcctga aagcgttcgt ttctccgcct gcttcggggc gaccgcacag tgccggggtg
    10801 aaccctccgg caggtgtgct tttcctgccc ctacgcgacg gctgcgaaac tcaccggctc
    10861 ctgagccttc ggtaaagact tccctccggc ccttggcttt tccgtttacc tctcacgctc
    10921 ttgcttatgt ttgtcatcct gagcgaagcg aaggatctgt tttttcgtga gtcgtgaatc
    10981 gtgagtcgtg aatcgaaagg tcaaaaccag attcccgccg gtaggcggac gttccgcttc
    11041 gcttcgctca g
//
LOCUS       DAOTIQ010000059        10726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_7307_length_10726_cov_6.996439, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000059 DAOTIQ010000000
VERSION     DAOTIQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7307_length_10726_cov_6.996439"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1035
                     /locus_tag="P5541_07495"
     CDS             205..1035
                     /locus_tag="P5541_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71866.1"
                     /translation="MRKKNLWAALAALFLLFVLSSESGAQNVHASGSQTRGTMGNSAR
                     LEGQPFYLPSPGTIISASSTGNGFWIQNAAGHVVARYWEANQAIGVVLAAGSYYIFPN
                     LAQGQNYAKAFALFNVGGAPPAAPGGIPGAAPGLVWVTSPRWGVSIMVPAGWVTRTDL
                     ANDVAIQAMEPGENAFIRVHAASGSGIKLEDLAVRFEGQRPYSIRRINQTVVNLSSGG
                     HAFVTEYSGSYNGQPIGAGFLLTHTPGRYYIVEAVYVQSASGLYANTLGQCMGTINTR
                     "
     gene            1104..3851
                     /locus_tag="P5541_07500"
     CDS             1104..3851
                     /locus_tag="P5541_07500"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV71867.1"
                     /translation="MENPKGFHPGRVPESGGNVALPHDDSDMSEVELSDVIDPSVIQR
                     MMDKFFKVTALPVSLIDTSGRILVGVGWQDICTGFHRVHPETCADCVESDTRLTEGIE
                     SGKFSLYKCKNGLWDTATPIIVEGKRLGNIFMGQFFFDDEEIDRDFFRAQAKRYGFKE
                     EEYMAVLDRVPRFSRETMENAMSFLVSLAGYLSEQGLANLRFARSNAENRRLLAEVST
                     REALQNLAGKLADLGWWVVDLETGTCTWSDEVCLIHERPTGFSPTVEQGISFYAPEYR
                     DRIREVFSKCAKEGIPYDEELQIISGSGRRIWVRTMGVPEKDASGNIVRVLGGFQDIS
                     ELKKAQEDLASSRERFDLFLNSSKDMAFLKDADMRLILANDAFLEFLGRAREGVIGRS
                     DYELLPAPLAEQCARGDEEVLRRGKPLVFEEHGGDRIYETMKFPVKFRNGTSGVGGFV
                     RDVTDLRKAEEALRESEERFRSLFENATIGLYRTSPDGRILMANPSVVKMLGYSSFEE
                     LARRDLSKDGFEPDYPREMFQQIMESEGEIIGLETAWTRRDGTVLFVRESARAVRGAD
                     GNTLYYEGTVEDITERKASEDALRESEAKHRRITENMTDVVWTADLDMRTTYISPSIE
                     RLLGESVGEHLERNMEDKFPPEDLARIHSIMAEEMEKEKAPDVDRERTRLIEARHYRA
                     DGSLIWISMHMSLIRDDNGVPIGIQGVTRDITRRVEVEEKLRKALQTTIEVLTQTVER
                     RDPYTAGHQRRVAGLAGAIASEMGFDQERVEAIRVAGYVHDIGKISVPSEILSKPGKL
                     SDIEKNIIREHPQSGRDILRDVDSGIPLAEIIYQHHERLDGSGYPRGLEGEDILIEAR
                     ILAVADVVEAMASYRPYRPALGIDEALDEIEKNRGTLYEPQVVDVCLSLFREKGYELV
                     E"
     gene            complement(3893..4789)
                     /locus_tag="P5541_07505"
     CDS             complement(3893..4789)
                     /locus_tag="P5541_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRV71868.1"
                     /translation="MPRVGLALGGGGARGAAHFGVLKVLEEEGVPIDIIAGTSMGAIV
                     GGMYAQNPDAGEIIKKYLSFLESMDYESLGLQNIVPQDEEEPHLLQKFAKTIARRVYI
                     SSMGSRSGIMKPDILEDALKTLLDRGSVRDAKIPFGAVATDLNSGNTLLLREGSLRKA
                     VKRSALIPGFLPPEEDGTRLITDGGVSDPIPVRSARDMGADMVIAVSVDPTTMASIED
                     PNMINIMRRCDLIRGIYMSRIQMEMADVCIWPDMSGAHWSEFLSSEEFIQAGEAETRK
                     KLPDIKKAIRRKRHWIFRFLPM"
     gene            4912..5208
                     /locus_tag="P5541_07510"
     CDS             4912..5208
                     /locus_tag="P5541_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71869.1"
                     /translation="MRFVMGVLGFIVANFIYSRVNMRTRLGLMWSGVATFTVLVFFTY
                     LATYYAPPEGLEEAVAAQRVAEMNARRIFLIAGEIAGMAFYSVKVVAEGRKGRP"
     gene            complement(5163..5930)
                     /locus_tag="P5541_07515"
     CDS             complement(5163..5930)
                     /locus_tag="P5541_07515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV71870.1"
                     /translation="MRKRNSVFAILTVLLMSVNPGPAKAAPNPVISGPAGVPVVALTF
                     DDGPHQRETPMLLDILEKHNVRATFFIVGTMAVNHPDILKEIVAMGHDVANHSWSHRN
                     MALLDRQAMYQEIAFCSQAIEALTGVRPKFFRPPGGNWNGALLETAYDLGLSLVLWSV
                     NAYDTRTKSAEEIASLVVRRAVPGSIILMHGGMENTMLAIPVIIDGLRSKGFLFATLD
                     QMFRFAPRARTPLPPRTTAETVSQGLPFLPSATTFTE"
     gene            complement(6007..7053)
                     /locus_tag="P5541_07520"
     CDS             complement(6007..7053)
                     /locus_tag="P5541_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRV71871.1"
                     /translation="MKPESAILFFTGIITIILTGAVLKTASGVFLPLVVAALLYFLFA
                     PIVKYMSSRKVPTPVTTTAVLAIFIGVCAAGGLFINTRIMAMISAYPRYHARFETLIG
                     TIMEQFEISPIWWENVHLAPVIGEQLLALSGFLVGFMTNLILVVFFLVFLLLGAPYFD
                     IKIDKALSSRNATRAREVTKKISGQISSYLFLQFVISAATGFIAWALFASVGLDFAVT
                     WGVLTFLLNFIPNLGSFIAAFPPILLALVQFYPSFGPAIIISTGLLIIQQGLGNFITP
                     KIMGDRLNLSPVIILVSLLMWGWLWGIAGAILSVPIASTIKIVCDNLSPLKPIGVLMS
                     SGKTFSRSQESREN"
     gene            complement(7197..7607)
                     /locus_tag="P5541_07525"
     CDS             complement(7197..7607)
                     /locus_tag="P5541_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HRV71872.1"
                     /translation="MLVDRARRYYLLEDMNCAESLIYAANDEYHLGLDETGLKTMAGF
                     GGGMGVETTCGALSGAIAAISVRFTKTKAHDNPEVREICKAFLENFLDKRGSIICGPL
                     KEMYRNESFRCMPVVEMAAEELEKIMKEVPEQNS"
     gene            complement(8091..8906)
                     /locus_tag="P5541_07530"
     CDS             complement(8091..8906)
                     /locus_tag="P5541_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRV71873.1"
                     /translation="MWQNEIRSASTLKGNIFTVPWKVCRDAAKGTDISPREAELWACE
                     NGICPSRYERSIGTIGLEGQARLLSSHVAVIGCGGLGGWIVEILARAGVGKLAVVDGD
                     MFGDNNLNRQALCTEENIGLSKAVAAGERALALNGAVEVDVWDQFMTGDNANAILEGC
                     DVVMDALDSNRARSVLVSACRELDIPLIHGAIGGFWGQSCVIMPDDRAPWEMTGGSDK
                     GIEETTGNPPFTPVFIASIQAAEAIRLITGIGEPLRDLLWCDLKEHEYLKIRI"
     gene            9041..9832
                     /gene="dapB"
                     /locus_tag="P5541_07535"
     CDS             9041..9832
                     /gene="dapB"
                     /locus_tag="P5541_07535"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRV71874.1"
                     /translation="MKKVFVSGASGNVGRLVVRKAITKDSTELVGGWCREQGLDLGTM
                     AGLTPVGVLSSGDLAEGLKESRPNVVVDFTSSNILMKNLLVYADEGVDAVIGTTGLSD
                     EDMERVVSMVVEKGLRWAVISNYGLGINLVMDFIGKARKHYPYVAITDRHHAGMANAP
                     SGTAATLAKAAGGYPAGEIKSSETFPGVLGAKISGIPVTSQRLPYPGPYSEHEITLAR
                     QDEIIRVTVQDFSSDIYMDGVFLAIEKVGGLPGGTLVRTLSELEG"
     gene            9894..10190
                     /locus_tag="P5541_07540"
     CDS             9894..10190
                     /locus_tag="P5541_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRV71875.1"
                     /translation="MIEKKHVEGEKKGEILIYALSTCGWCRKTKDHLKEMGLAYDYID
                     VDLLDPDESEEVMKIVRKHNERGSFPTIVVNGEHCVVGYDIDKVKELLGDGSGE"
     gene            10174..10707
                     /locus_tag="P5541_07545"
     CDS             10174..10707
                     /locus_tag="P5541_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRV71876.1"
                     /translation="MDQENKKRYIPSDEEKESLYRKLKEEAEQSGYFLGPDRELVMTL
                     MHGLLVNQERFGYMSCPCRLASGKIDEDRDMICPCDYRDPDLDEYETCYCSLYVSERV
                     AKGEHEVGSIPERRPSREERLAAAEKKETSGKEITGGPAYPVWRCKVCGYLAARDNPP
                     GVCPICKATRERFERFV"
BASE COUNT         2510 a         2749 c         3084 g         2383 t
ORIGIN      
        1 ccggctgaag cgtaggttac gaaaaccggt ttcatctgat cccccctcaa taacgtccga
       61 atttcagtta aaagtctagg gtattcctgt aacgccagtt ttgttttact gtttcagtta
      121 tgtcacaggg ggattttttt ggttcgcagt gataaaatgg aggacgttaa taaattatcc
      181 gctttttctg ggagagtgaa gataatgaga aagaaaaacc tgtgggccgc ccttgcggca
      241 ctttttcttc tttttgtgct ttcatccgaa tcgggagcgc agaatgtcca cgcctcgggt
      301 tcacagactc gcgggactat gggtaactct gcccgacttg agggtcagcc cttctatctt
      361 ccgtcacccg ggacgataat ttccgcatca tctaccggta acgggttctg gatccagaat
      421 gcggctgggc atgtcgttgc cagatactgg gaagcgaacc aggcaatagg tgttgtcctg
      481 gcggccggtt catactatat tttccccaac cttgcgcagg ggcagaatta tgccaaggcc
      541 ttcgcgcttt tcaatgtagg cggcgctcct cctgccgccc ccgggggaat tcccggagcg
      601 gctccgggtc tggtatgggt aacaagtccc aggtgggggg tatccataat ggtcccggcc
      661 ggttgggtca cgaggaccga tcttgccaac gatgttgcta tccaggcaat ggagccgggt
      721 gagaatgcct tcatcagggt ccatgccgcc agtggctcgg ggatcaaact ggaagatctt
      781 gccgtccggt ttgaggggca gcgcccctac agtatcagac gcatcaacca gacggtggtc
      841 aacctcagca gcggcgggca cgcttttgta accgagtatt cggggtctta caacggccag
      901 ccgataggcg cagggttcct cctgactcac acgccgggca ggtactacat tgtcgaggcc
      961 gtatatgtac agtccgcgtc tggcctttac gccaacaccc ttggccagtg tatgggtacc
     1021 atcaacacga ggtaatgtta aaatgatcgg gatctttcag ggagatggtc tcacgctgta
     1081 gagagtagag agggtggcac tgaatggaaa atcccaaggg cttccatcct ggccgggttc
     1141 cggaatctgg tggaaatgtg gctcttcccc atgacgatag tgatatgtca gaggtagagc
     1201 tttctgatgt aattgatcct tcggttatcc agagaatgat ggacaaattc ttcaaggtga
     1261 ccgctctccc ggtgtctctt atagatacca gcgggagaat tctcgtgggc gtaggctggc
     1321 aggatatttg caccggtttc cacagggtac atcctgagac atgcgcggac tgcgtcgaga
     1381 gtgatacgag gcttaccgaa gggatcgaat cggggaaatt ctccctctac aagtgcaaga
     1441 acggcctgtg ggacacggct acgcccataa ttgtagaagg gaaaaggctg ggaaacatct
     1501 ttatgggcca gtttttcttt gatgacgagg agatcgaccg cgattttttc cgtgcccagg
     1561 caaaacgcta cggtttcaaa gaggaagaat atatggcggt ccttgacagg gtcccacggt
     1621 tttccagaga gaccatggaa aatgcaatgt cctttttggt gtccctcgcg ggatacctct
     1681 cggaacaggg tctggccaac ctcaggtttg cccgttcaaa cgccgagaac cgcaggttgc
     1741 tggcggaggt ttcaaccagg gaagccttac agaatcttgc cgggaaactg gccgacctgg
     1801 ggtggtgggt cgtcgatctt gagaccggta cctgtacctg gtccgacgaa gtttgtctca
     1861 tacacgaaag gcccaccggt tttagcccga ctgttgagca gggtatctcc ttctacgccc
     1921 ccgagtacag ggacaggatc agggaagtct tcagcaaatg tgccaaagag ggcatcccct
     1981 atgacgaaga actgcagatc atttccggaa gcggccgcag gatctgggtc aggacgatgg
     2041 gtgttcccga gaaggatgcc tccgggaata tcgtccgggt ccttgggggt ttccaggaca
     2101 tctcggaact gaaaaaagca caggaggacc tggcctccag ccgcgagagg tttgaccttt
     2161 tcctcaattc cagcaaggat atggcctttc tgaaggatgc agacatgcgt ctgatcctgg
     2221 caaatgacgc cttcctggag ttcctgggca gggcaaggga aggggttatc ggaaggagcg
     2281 attacgaact tctgcctgcc ccccttgccg agcagtgtgc caggggtgac gaagaggtcc
     2341 tgcgtagagg gaaacctctg gttttcgaag aacacggagg agacaggatc tacgagacga
     2401 tgaaattccc cgtcaaattc aggaacggga cctctggggt cgggggattc gtccgcgatg
     2461 taacggacct ccggaaggcc gaggaggccc ttcgggagag cgaggagaga ttccgaagtc
     2521 tcttcgagaa cgcgactata ggcctgtaca ggacaagccc cgacggaagg atcctgatgg
     2581 ctaatccctc ggtcgtaaag atgctgggat attcgtcatt tgaagaactg gcaaggcgcg
     2641 atctcagcaa agacggtttc gaacccgact acccccggga aatgttccag cagatcatgg
     2701 agagcgaagg agagataatc ggcctggaaa cggcctggac cagaagggac ggcacggtgc
     2761 tctttgtccg ggagagtgcc agggctgtac gcggcgctga cggaaacacc ctctattacg
     2821 aggggaccgt cgaggatata acggagcgca aggcatccga ggacgccctg cgtgagagcg
     2881 aggcaaagca ccgccggata acggagaaca tgaccgacgt ggtatggacc gccgatctgg
     2941 acatgaggac cacttacata agcccctcca tagagaggct cctcggggaa tcagtcgggg
     3001 agcaccttga gcgaaatatg gaggataagt ttccccccga agaccttgcc aggatccatt
     3061 ccataatggc tgaggagatg gaaaaggaaa aagccccgga tgttgacagg gagaggacca
     3121 ggcttatcga ggccaggcat tacagggctg acggaagcct gatatggatc tccatgcaca
     3181 tgtccctaat ccgggatgat aatggtgtcc ccataggtat ccagggggtg accagggata
     3241 ttaccaggag ggtcgaggtc gaagaaaaac ttcggaaggc tctccagacg accatcgagg
     3301 ttcttacaca gaccgttgaa agaagggacc cctatactgc cggccaccag aggcgcgtcg
     3361 ccggccttgc cggggccatc gcttctgaga tgggtttcga tcaggaaagg gttgaagcca
     3421 taagggtcgc gggatatgtc cacgatatcg gaaagatttc cgtaccttcg gagattctca
     3481 gcaagccggg gaagctctcg gatattgaga aaaatattat tcgcgaacac ccacagagcg
     3541 gcagggatat ccttagggat gtggattcgg gcattcctct tgccgagatc atttaccagc
     3601 atcacgaaag actggacggc tcagggtacc cgcggggtct tgagggcgag gatatcctca
     3661 ttgaggcccg aatcctggca gtagccgacg tagttgaggc aatggcatcc tacaggccct
     3721 accgccccgc tcttggaatt gacgaggccc tggatgaaat cgagaagaac aggggaaccc
     3781 tttacgaacc gcaggtcgtg gatgtatgcc tgagtctgtt tcgagagaag ggctacgaac
     3841 tggtcgagtg atgtaaggag taattcttca acagacagga gtatctgttg aattacatcg
     3901 gcaggaacct gaatatccag tgtctctttc ttctgatggc tttttttatg tcgggtaatt
     3961 tctttctggt ctctgcctct ccggcctgga tgaattcctc cgaagagaga aattcgctcc
     4021 aatgtgctcc gctcatgtcc ggccagatgc agacatcggc catctccatc tggattctgg
     4081 acatgtagat gccccggatc aggtcgcacc tgcgcatgat gtttatcatg ttgggatcct
     4141 ctatgcttgc catggtggtg ggatccacgg aaaccgcgat caccatgtcc gcccccatat
     4201 ccctggcgga tctgaccggg atcgggtcgc tgacaccccc gtcggttatc aatcttgtgc
     4261 cgtcctcttc gggggggaga aagccgggta tcagggcgct tctttttacg gcctttctca
     4321 ggctcccctc acggaggaga agtgtgttcc cgctgttaag atctgtcgca acggctccga
     4381 agggtatctt cgcgtccctg acgcttcccc tgtccagaag cgttttgagc gcgtcctcca
     4441 gaatgtccgg tttcattatt ccggaccgtg accccattga actgatgtag actcttctgg
     4501 ctatggtctt ggcgaatttc tggaggaggt ggggctcctc ttcatcttga gggactatgt
     4561 tctgaaggcc aagagactcg taatccatgc tttcgaggaa agagaggtat ttttttatta
     4621 tctcgccggc atcggggttc tgggcgtaca ttcccccgac gatggctccc atagaggttc
     4681 cggcgataat gtcgatggga actccttctt cttcaaggac tttcaggaca ccgaaatgag
     4741 ccgcgcccct ggctcctccc cctccgaggg caaggcctac tctgggcaaa ataaagcccc
     4801 cctttgcaat aaatgacatt tcacgtaaat atataacacg gtttcgggaa gttgtgaaat
     4861 tgaacggttt tccggtaaca ggaaaaacga ataatgactg gagggatcct tttgagattt
     4921 gtcatgggcg ttctcggatt cattgtcgcc aatttcatct attcccgggt caacatgagg
     4981 acacgccttg ggctgatgtg gtccggagtg gccacgttca cggtactggt gttttttacc
     5041 tatcttgcca cctattacgc ccctcccgaa ggacttgagg aggccgtggc agcccagagg
     5101 gtggcggaaa tgaacgccag gaggatcttt ctcattgctg gagagatcgc agggatggct
     5161 ttttattctg taaaggtagt ggctgagggc agaaaaggaa ggccctgaga aacggtttcg
     5221 gcagttgtcc gggggggcag gggggtcctt gcccgggggg caaaacggaa catctggtcc
     5281 agggtggcga aaaggaaccc cttgctcctg agtccatcaa tgatcacggg aatggcaagc
     5341 atggtgttct ccattccgcc atgcatcagt atgatcgaac cgggaaccgc gcgcctgacg
     5401 accagtgacg cgatttcctc tgcgcttttg gtcctggtat cgtaggcgtt cacgctccac
     5461 aggaccagag aaagcccgag gtcgtaagcg gtctcaagga gagcaccgtt ccagttacca
     5521 ccgggggggc ggaaaaattt cgggcgaact cccgttagtg cctctatggc ctggctgcag
     5581 aaggctatct cctggtacat ggcctgcctg tcgagaaggg ccatgtttct gtgggaccag
     5641 gaatggttgg ctacatcatg tcccatggcg acaatttcct tcaggatatc ggggtgattt
     5701 acggccattg tcccgacgat aaagaaagtt gctcttacat tgtgtttctc gagaatgtcg
     5761 agaagcatgg gggtctctct ctggtggggc ccgtcatcga aagtaagcgc taccaccgga
     5821 acacccgccg gtccggatat caccgggttc ggtgcagcct ttgccgggcc tgggttgacc
     5881 gacataagca ggacagtcag aatagcgaaa acagagtttc ttttcctcat ttccctgctc
     5941 tcccttgaag actttagcaa gacaatataa cgcaagaact caaaagtcaa caggaggaca
     6001 ggatagtcag ttttctctgg attcctggga tctggagaag gtttttccag aggacataag
     6061 gacacctatt ggcttcaggg gagacagatt gtcgcacact atctttatcg tcgatgctat
     6121 cgggaccgag aggattgcac ccgcgatacc ccagagccat ccccacataa gcagggagac
     6181 gagtatgatc acggggctca ggttgagccg gtctcccatg atctttgggg taataaaatt
     6241 gcccagcccc tgctggatta tcagaagacc tgtagagatg atgattgccg gcccgaaaga
     6301 gggataaaac tggaccagag caagaagaat cggggggaag gccgctatga aagaaccaag
     6361 gtttgggatg aagttgagca ggaatgtcaa caccccccag gtaaccgcaa aatccagccc
     6421 gaccgacgca aaaagggccc atgctatgaa acccgtagcg gcgcttatga caaactgcag
     6481 gaaaagatac gaactgatct gacccgatat ctttttggtc acttcccggg ctcttgtcgc
     6541 gttgcgggaa gatagagcct tgtcgatctt gatgtcgaaa tagggtgccc ccagaagcag
     6601 aaaaaccagg aagaagacca ccaggataag gtttgtcatg aatccaacca ggaatcccga
     6661 aagggcgagc agttgttcgc cgatcaccgg tgccaggtgg acattctccc accagatggg
     6721 tgagatctcg aactgttcca taattgtccc gataagggtc tcgaacctgg cgtggtaacg
     6781 cggatacgcc gaaatcatcg ccatgatccg ggtattgatg aacagccctc cagcagcgca
     6841 tacgccgatg aagatggcca ggaccgccgt ggtcgtcacg ggagtcggga ctttcctgct
     6901 ggacatgtac ttcacgatcg gggcgaaaag gaagtacagc agtgctgcaa cgacgagagg
     6961 aagaaagact ccactcgcgg ttttcagcac tgcacccgtc aggatgattg tgatgattcc
     7021 ggtaaaaaac agtattgcac tctcaggttt caattgcagg cctccaaaaa agattgtgaa
     7081 tcgtgaatcg tgagtcgtga atcgaaaatc gaatggttca aaggcgtgaa cagtcaacgg
     7141 taaacggtta acggtgtgaa gcgaaaaggc gaggacaagc cacttgccct tgccgttcac
     7201 gagttctgtt ctggcacctc tttcatgatc ttttccagtt cctccgcggc catttcgacg
     7261 acaggcatgc agcggaaact ttcgttcctg tacatctcct tcagaggacc gcaaatgatg
     7321 gagccccttt tgtccagaaa attttccagg aaggccttgc atatctctct gacctcaggg
     7381 ttatcgtggg cctttgtttt tgtgaaccgg acggatatgg cggcaatagc tccggaaagg
     7441 gctccgcagg tggtctcaac ccccatgccc ccgccaaagc cggccatagt cttgaggccg
     7501 gtttcgtcca gtccaaggtg gtattcatca tttgccgcat agataaggct ctcggcgcag
     7561 ttcatatcct caagaagata atagcgtctt gccctgtcaa caagcatccc gaaacctcct
     7621 gatcatgcgt taatcgtgaa ccgtgaatcg aaaaaaccgg gagacaggag actggcaaca
     7681 ggatactgga gggacaattg cctgtcattc tgagcgaagc gaagcggaat ccgccagcag
     7741 gcggacgttc cgcgcccttt gcctaccggc gggaatctgg ttcaaattcc accaggatac
     7801 tgccggtccc ggagttaagt ctttcccgaa aggcaactcg cctgaaatcg ttcgtttctc
     7861 cgcctgctac ggggcgatcg cacagtgccg ggaaaaaccc cggcaggtgt gctttccctg
     7921 tccctccgcg acggcttcga aactcaccgg ctcctgagcc ttcggtaaag acttctctcc
     7981 ggctccgccc ttccagcctc cggtctccag acgccagtct cctgtatcct gacgccttgt
     8041 ggttttcacc gttcaccgtt cacgcctttg attctgagcc tccccgcctc tcagatcctg
     8101 atctttagat actcatgctc cttaaggtcg caccagagca ggtctctcaa tggctcgccg
     8161 attccggtta tcagtcttat ggcttcggcg gcctgaatgg aggcaatgaa tacaggtgta
     8221 aagggcgggt tgcccgtcgt ctcttctata cctttgtccg atcccccggt catctcccag
     8281 ggagccctgt catctggcat gatgacgcat gattgccccc agaaaccgcc tattgctcca
     8341 tggatgagag gtatatccag ttcccggcag gcggaaacca gcaccgaacg agccctgttg
     8401 ctgtcgagag cgtccatgac aacatcgcaa ccctcaagta tcgcgttagc attgtctccg
     8461 gtcatgaact ggtcccacac gtcaacttcc acggcaccgt tgagggccag tgccctctct
     8521 cccgccgcaa cggcctttga aaggccgata ttttcctccg tgcaaagggc ctgcctgttg
     8581 aggttattgt cgccgaacat gtctccatcg acaacagcaa gtttgccgac cccggcccgt
     8641 gcaagtattt cgactatcca gccccccaga cctccgcagc cgataaccgc cacgtgcgaa
     8701 gaaaggagcc ttgcctggcc ttccagccct attgttccta tgctgcgctc gtagcgcgag
     8761 ggacagatcc cgttctcgca ggcccagagt tcggcttccc ggggagatat atccgtccct
     8821 ttcgctgcat cacggcagac tttccatggg actgtaaaga tattgccctt cagagtgctt
     8881 gccgacctga tttcattttg ccacaaaaca gccatttcca ccgcacccct ctttgttgcg
     8941 cggattttga catcaaggat accatcaggt gaccatgcag cacaaattag ggtaaactgt
     9001 cacttatctg cagaaaaaca tttgggggag gtgatccatc ctgaaaaaag tctttgtatc
     9061 cggagcttca ggaaatgtcg gcaggctggt cgtaaggaaa gcaataacga aggatagcac
     9121 ggaattggtc ggaggatggt gccgggagca gggattggac ctgggaacca tggcaggtct
     9181 gacgccggtc ggggttctct cgtcggggga tctcgcggag ggtttaaaag aaagtcgccc
     9241 caatgttgtg gtggatttta cgtccagcaa catccttatg aaaaacctcc tggtctacgc
     9301 tgatgaagga gtcgatgctg tgatcggtac caccggcctt tctgatgagg atatggagag
     9361 agtggtctcc atggtggtgg agaaggggct tcgatgggcc gtaatctcca actacgggct
     9421 ggggatcaat ctcgttatgg attttatcgg aaaggcaagg aaacactatc cctacgtcgc
     9481 catcactgac aggcatcatg cgggaatggc caacgcgccc agcgggacgg cggccactct
     9541 cgcgaaagcg gccggagggt atcccgcagg ggagatcaaa agcagtgaga ccttccccgg
     9601 ggtgctgggg gcaaagatat ccgggattcc tgtgacctcc cagaggcttc cctacccggg
     9661 gccgtactcg gaacacgaga taacccttgc acgtcaggac gagataatca gggtaactgt
     9721 gcaggacttt tccagtgata tttacatgga cggagtgttc ctggcgatag aaaaggtcgg
     9781 gggacttcca gggggcacgc tggtgaggac cctttccgaa ctggaaggct gagccgggag
     9841 ataaacagga ttaacgaccc ggttcaccaa atctgatgcg gggagcgata gagatgatcg
     9901 agaaaaagca cgttgaaggc gaaaagaagg gcgagatact gatctacgca ttgagcacgt
     9961 gcggctggtg caggaagaca aaggatcacc tcaaggaaat ggggcttgcc tacgactata
    10021 ttgatgtgga cctgctggac ccggatgagt ccgaagaggt gatgaagata gtcaggaagc
    10081 acaacgaaag gggttccttc ccgacaatag ttgtaaacgg cgagcactgc gtcgttggtt
    10141 acgacatcga caaggtaaag gagcttctgg gggatggatc aggagaataa aaaaagatat
    10201 atcccctctg acgaggagaa agagtctctt taccggaaac tgaaggagga ggcggagcag
    10261 tccgggtact ttctcggtcc tgatcgggag ctggtaatga cccttatgca cggccttctg
    10321 gttaaccagg agcgctttgg ctacatgtca tgtccctgca ggcttgcctc ggggaagatc
    10381 gacgaggacc gggacatgat ctgtccctgc gattaccggg atccggatct ggatgagtac
    10441 gagacctgct actgcagcct ctacgtgtcg gaaagggtcg caaagggaga acatgaggtc
    10501 ggctcgatcc cggagaggcg tccgtcccgt gaagagcgtc tcgccgcggc tgaaaagaaa
    10561 gaaacctctg ggaaagaaat tacgggaggc cctgcgtacc ctgtctggcg atgcaaggta
    10621 tgcggttacc tggccgcacg agacaaccct cccggagtat gcccgatctg caaggcaacc
    10681 agggagcggt ttgagaggtt tgtgtaattg gatcgtaacg cgtgac
//
LOCUS       DAOTIQ010000060        10206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_7801_length_10206_cov_8.159590, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000060 DAOTIQ010000000
VERSION     DAOTIQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_10206_cov_8.159590"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..689
                     /locus_tag="P5541_07550"
     CDS             84..689
                     /locus_tag="P5541_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRV71877.1"
                     /translation="MDEVNFVTLIPYVFITSFTPGPNNISCAAMAARWGMRRTWGYLS
                     GIGAGFFVLLLFCGAFSGVLMKLLPWLQNYMKWVGAAYILWLAWGIATSDSHGSVEEG
                     SVSLRGFVKGFALQFVNPKAILYGVTLYTAFLGPILARPVPVFVWSIFLTLIGFSSIL
                     SWSLFGLGIEKFLQSPFQRKLLNGCFALLLVYAALGVAGLI"
     gene            complement(736..1170)
                     /locus_tag="P5541_07555"
     CDS             complement(736..1170)
                     /locus_tag="P5541_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV71878.1"
                     /translation="MGVKALILGEEGADSPKLVEIAGPSAAEGFMIVTNLNRDDERPV
                     VQNFIREFKARYGIDPDMVGASAYDGLMIIVDAMRRAGTTDAAKVRDAIAATSDFNGL
                     TGVIKGFTPIGEVVKPVQVQIIKMGEYRYFAEITDPEIITPR"
     gene            1552..2658
                     /locus_tag="P5541_07560"
     CDS             1552..2658
                     /locus_tag="P5541_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRV71879.1"
                     /translation="MPGRGQVVLDLEDSRIVIPTDRRLRKVYLEFSSRCNLNCPMCFR
                     ESWTEPQGDMHRDLFLRFFSGLGDFPGLEAVHFLGIGEPFCNPGLFEAVRMLKSLNLQ
                     VRITTNATMIDRAVAEDLVASGVDILDISVEAENVPWPGRTSSLDILSGVAEDLSGAK
                     KRAGSYSPDIRVALVVTRSNLSRIPDIVGAIRGKGISAASVSNLLPTDMEADKNRLYP
                     ASLEEEVAIRSKLYTLFQYSGIKPELPRFALSTERHCAFVKSESCFIRWDGLVAPCLR
                     LSHGYSEVVLGRKKEVVPWFFGDLGNQTLCEIWDGSEFTSFRWRLRRALYASCLDCNL
                     LRSCEYAKTTESDCWGDSPGCSDCLWDRNLIRCP"
     gene            2803..4599
                     /locus_tag="P5541_07565"
     CDS             2803..4599
                     /locus_tag="P5541_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRV71880.1"
                     /translation="MVEKKVLRVNLTKGTVKEEPVNKEWMKQFIGGRGYATKMLYEEL
                     KKGTDPLSKDNILIFANGPLTGSNVPTGGRYMVVTKSPLTGMIASSNSGGYWGNELAR
                     AGYMFLVFEGKAEKPVYLWINDGKAELKDASSLWGKDTHETTDMILKEIGDAKAKVAC
                     IGPAGEKLSPISCVINDKHRAAGRSGVGAVMGSKNLKAVAVKGSGTFKASDPEGLKEV
                     LRAGMQKIKENGVTGEGLPTYGTAVLVNILNQLGSYPHKNFQESMDDQAEKHSGERLA
                     ETFLTGRTNCFACPIGCGRETALDGKKGEGPEYETIWAMGSNCGIADLEPIIRANYLC
                     NELGLDTISAGGTISAAMELYEKGYIKESELGRGPELRFGSGDALVYWVEKMGKSEGL
                     GEKLAMGSHRLCEAYGHPEFSITVKKQELPAYDPRGIQGIGLTYAVSNRGACHVRGYL
                     ISPEVLGIPEKLDPWEIDGKPMWGKIFHDLTAAIDSSGMCLFTSFALGAEDYAAMLKH
                     GAGFDICPESLLKIGDRIWTLERLFNLREGLDPAKDDTLPVRLLNEPIPDGPAKGKLS
                     RIKEMIPEYYKIRGWDAKGVPTKDTLVMLGLN"
     gene            4612..4884
                     /locus_tag="P5541_07570"
     CDS             4612..4884
                     /locus_tag="P5541_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HRV71881.1"
                     /translation="MTKVSFFGMVRDIIGEKETQVEGGYSVESLLEALSLKYGLTFGE
                     KVLKMPERDMDLIILLNGRHIEHVGGLSAPLKEGDHLAVFPLVGGG"
     gene            5004..5261
                     /locus_tag="P5541_07575"
     CDS             5004..5261
                     /locus_tag="P5541_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71882.1"
                     /translation="MRREKIEMRGVSLDEISSHFRETGWQEVSPGLWQRDCDSVEAGL
                     QEEVSLGSIRLPSVTLVFRLRRGDHEKVLSAFRSRFLKAGG"
     gene            5280..6470
                     /locus_tag="P5541_07580"
     CDS             5280..6470
                     /locus_tag="P5541_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71883.1"
                     /translation="MVTRKIELNTGIVAPGVGFGSSAIPTEPHRRIMGHYASPLLLGP
                     DPSQDLLEMVMHMFSDEEAQVAQYLPPLRPRTAEAVSARAGHTLDETSRILDSLAHKK
                     RVILASGSPRKYTILPIVPGTFEMALMTPDLSTRTRWHADFARLFEKIWDEGYIVRYT
                     RSIRPGIRYLPVHNSVPSLLKACPSERMEEMVRPYSDFAVGMCQCRMAMTLVDKGCGR
                     PMENCVVMGPGAQKFIDGGLMRRTDAAEVLAIKRHAEENGCVSWSMSDVGGVPRGNSS
                     CSCCGCCCHAMRSISQFNFPAMISPPVFMPILDPAKCVNCGACVVACPMNAWSSDGSS
                     LLFDPARCIGCGLCVVACKKGAMTLGESGNRLKNAPGWFGFFLDNAPGYLLNAFRVWA
                     RRHL"
     gene            6530..8929
                     /locus_tag="P5541_07585"
     CDS             6530..8929
                     /locus_tag="P5541_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRV71884.1"
                     /translation="MKDFEKMGSFYLGKRVDDASGSMTGEMILYDAKDLTTHGIIIGM
                     TGSGKTGLALSLIEEALMDNIPVIAIDPKGDIPNLLLTFPQFRPEDFRPWISEQEAAR
                     KGLSPDEFSAKTASDWKNGLAEWGIDGARIEKMRNSVDMTVYTPGSSAGRMVSILHSF
                     DAPPEAIRSDGDLFRERVQTTASSLLALMGIDADPLTSREHILISKILEEKWNAGQSL
                     DIPSLIDLIRVPPFKGMGVLDLETFYPSSSRMDLVLRLNNLVAAPSFGPWMEGEPLDA
                     AKFLYTADGRPRASIFTISHLSERERMFFVSLLLNEILGWVRTQPGTSSLRAILYMDE
                     IFGFFPPVQNPPSKTVLLTLLKQARAYGLGVLLATQNPVDLDYKGISNTGTWFIGRLQ
                     TERDKARILEGLKGVSEKGFPTQELDRMISGLGKRVFLLHNVHEDEPVLFQTRWAMSY
                     LAGPVTREQIRMIEGPHAAVPTRGRGEARAPSVSAAPRPVQEIIPSDIVSVFLPVSPG
                     IDAEYAPFLGGFARIHFRDSKLGVAETKMVAFATEMSEGPLTVDWESAFEISVDPAVL
                     SQGMPPSVMSPVPTAGLDSKSYTKWGRDFLKWLPNNSLLTVMTCPSMKEAARPGESKA
                     DFAARLALSRREQRDAAVEKIRKKYASKLTTLQNRLMRAEQAVEREAEQAKSRKMDTA
                     ISFGTAIAGAFLGRKASVYSASRMGTAVSKAARLSKESGDVARAKETVESVRLEIAGM
                     ERQLEDEINATGFGDILETGIEESFIRPKASDMSVVLFGLLWLPYARNPQGGLSPLWG
                     D"
     gene            9013..9393
                     /locus_tag="P5541_07590"
     CDS             9013..9393
                     /locus_tag="P5541_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRV71885.1"
                     /translation="MRKTVATDKAPAAIGPYSQAVWANDILFVSGQIPIDPKSGEMSG
                     KTAAEQAEQVLKNVGAILESQGLSFASVVKATVFAKDMNDFKAVNDVYASYFTADPPA
                     RAFVEVARLPKDALVEIEVIAVKG"
     gene            9482..9682
                     /locus_tag="P5541_07595"
     CDS             9482..9682
                     /locus_tag="P5541_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRV71886.1"
                     /translation="MQGTVKWFNEAKGYGFITPDEGNDVFVHFSAIQGDGFKTLAEGQ
                     RVEFEVVQGDKGPQAANVEKIS"
     gene            9863..10138
                     /locus_tag="P5541_07600"
     CDS             9863..10138
                     /locus_tag="P5541_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRV71887.1"
                     /translation="MNKSELIQAVAKSAGLSNSKATQVVSAVIDEIGAALAGGDKVQV
                     AGFGTFEVRQRAAREARNPQDPTKKVKVPARKVPAFRAGKALKDKVC"
BASE COUNT         2313 a         2699 c         2998 g         2196 t
ORIGIN      
        1 aacactagga ctcaaccccg acagtggact ttacacaaaa tattacgcac tctccggaca
       61 aacaaaagca aggagagatc cttttggatg aggttaactt tgttaccctg ataccatacg
      121 ttttcatcac ttcctttaca ccagggccga acaacatcag ttgtgctgcc atggcggcac
      181 gctggggtat gaggcggacc tgggggtacc tttccggtat aggggcgggg ttttttgtcc
      241 tgcttctttt ctgcggtgcc ttttcggggg tcctgatgaa acttctcccg tggttgcaga
      301 attacatgaa gtgggtcggg gccgcataca ttctctggct tgcctggggc attgccacca
      361 gtgatagcca cgggagtgta gaggaaggat ctgtttcatt gaggggcttc gtcaagggtt
      421 tcgcccttca gttcgtcaac cccaaggcca ttctttatgg tgttaccctt tatacggcct
      481 tcctgggccc catactggca cgtccggtgc ccgtcttcgt gtggtccatt tttctgacgc
      541 tgataggatt ttcttccatc ctttcctggt cccttttcgg ccttggcata gaaaaattcc
      601 tgcagagccc tttccagaga aaactcctca acggctgttt cgccctgctt cttgtctatg
      661 ctgccctcgg tgtcgcaggt ctgatctgaa agaaaaagcc ggggcagaaa gccccggctt
      721 ttttgttaac gtaaactacc tgggtgtgat aatttcggga tcggtgatct cggcaaagta
      781 cctgtactcg cccattttga ttatctggac ctgcacgggt tttacgactt cgccgatggg
      841 ggtgaatccc ttgatgacgc cggtgagtcc gttgaagtcg ctagtagccg ctattgcgtc
      901 gcgaaccttc gccgcgtcgg tggtaccggc ccttctcatg gcatcgacga taatcatcag
      961 tccgtcatag gccgaggctc cgaccatgtc ggggtcgatc ccgtagcggg ctttgaactc
     1021 cctgatgaag ttctgcacga cggggcgttc gtcatcacgg ttgaggttgg tgacgatcat
     1081 gaatccctcg gctgctgaag gtcccgcgat ctccaccagt ttcggtgaat ctgctccctc
     1141 ttcaccgagg ataagggcct ttacgcccat ctccttggcc tgtttcagga tagtgccagt
     1201 ccggaagtta tgagcgcgta aataaaacct atggcaatac cgttgagaag ctgttcaaga
     1261 aaaagttcca atgatcgtcc tccagatgga taaagataga aagatctttg tcagggccct
     1321 tcaaggaaag agaacatgca tcgggcactt aataataata aaccattttc ttctgatctt
     1381 gcattctgat agcgaggttt tttgaatctg aagaaacgcg gatgatccat gtcgggtccc
     1441 tcccgaaaaa aacgacacca tgtatcactt ctccttgact cattcccttg ttgccctgac
     1501 aataggtcat atattgtaag cgcttttatg tatctgcctg ggggagagcc gatgccggga
     1561 agaggacagg tcgtgcttga tcttgaagac agccgcatcg tcataccaac tgaccgccgt
     1621 ctccgcaagg tctacctgga gttcagttcc cggtgcaacc tcaactgccc catgtgtttc
     1681 cgcgaatcct ggaccgaacc ccagggagac atgcatcggg acctgttcct gcgctttttt
     1741 tcgggcctcg gagattttcc cgggcttgag gccgtccatt tcctggggat cggggagccc
     1801 ttttgcaatc cgggactttt cgaggcagtc aggatgctga aatcgctaaa cctccaggtc
     1861 cggatcacta ccaacgccac aatgatagac cgggccgttg ccgaggacct tgtagcctcg
     1921 ggtgttgata ttctggacat ttccgtcgaa gcggagaatg tcccctggcc gggcaggaca
     1981 agcagtctcg atatcctttc aggagtcgcg gaggatctat ccggagcaaa gaaaagggca
     2041 ggctcttact caccggatat ccgcgtcgct cttgtagtga cgagatccaa cctttcccgc
     2101 atacccgata tcgtcggtgc catccgcgga aagggaatat cggccgcttc ggtgagcaac
     2161 cttctaccga cagatatgga agccgataaa aacaggcttt accccgcctc ccttgaagag
     2221 gaagtagcga taaggtcgaa actctatacc ctctttcagt attcggggat caagccggaa
     2281 ctcccgagat tcgctctttc gacggagcgg cattgtgctt tcgtaaaatc cgagtcctgt
     2341 tttatccgct gggatggcct tgtggcccct tgcctgagat tgagccatgg atacagcgag
     2401 gtcgttctcg ggagaaagaa ggaggttgtc ccgtggtttt tcggggatct tggcaatcag
     2461 accctttgcg agatctggga tggttccgag ttcaccagtt ttcgatggag gctgcgcagg
     2521 gccctatacg cgtcctgtct cgattgtaac ctgctcaggt cctgtgagta cgccaagacc
     2581 accgaaagcg actgttgggg agactccccc ggctgcagcg actgtctttg ggacaggaac
     2641 ctcatcagat gtccttgacg tttatatccc cggtgatgaa aggagagtga taagagaaag
     2701 gtgccggaaa aggaaacttc catacccgag aacagagagc cgacagagaa atgagcctgg
     2761 agaagcgtta aagatcgtga atatcagaga ggaggaaagg gcatggtaga aaagaaggtc
     2821 ctgagggtca acctcacgaa ggggacggta aaagaggaac cggtcaacaa ggaatggatg
     2881 aagcaattca tcggagggcg cggatatgcc accaaaatgc tctatgaaga gctcaagaaa
     2941 gggacggatc cgctctcgaa agacaacatc ctgattttcg ccaacggtcc tctgaccggc
     3001 tccaacgttc ctaccggcgg caggtacatg gtggttacaa agagccccct cactggcatg
     3061 atcgcgtcaa gcaactccgg tgggtactgg ggcaacgagc tggccagggc ggggtacatg
     3121 ttcctcgtct ttgagggaaa ggccgaaaaa cccgtctatc tctggataaa cgacggcaag
     3181 gccgaactga aggatgcctc ttcactttgg ggcaaggata cccatgagac gacggacatg
     3241 atcctcaagg agatcgggga tgcaaaggca aaagtagcct gcataggtcc tgcaggagaa
     3301 aaactttcgc ccatttcctg cgtcatcaac gacaagcacc gcgcggcggg aagaagcgga
     3361 gtgggcgccg tgatgggctc caagaacctc aaggccgtgg cagtaaaggg cagtgggacc
     3421 ttcaaggctt ccgacccgga aggactcaag gaggttctcc gcgcggggat gcagaagatc
     3481 aaggagaacg gcgtaaccgg ggagggactg ccgacctacg gaacggcggt cctggtcaac
     3541 atcctcaatc agctcggcag ttatccccac aagaatttcc aggaatccat ggatgaccag
     3601 gccgaaaaac actccggcga gaggctcgcc gagaccttcc ttaccggcag gacgaattgt
     3661 tttgcctgcc ccattggatg cggccgggag accgccctgg acggcaagaa gggtgaaggc
     3721 ccggaatacg agactatctg ggccatgggg agcaactgcg gtattgccga tctggaaccg
     3781 atcatccgtg ccaactacct gtgcaacgaa ctgggccttg acacgataag cgcgggtggg
     3841 accatctccg ccgcgatgga actttatgaa aaagggtata tcaaggaatc cgaactgggt
     3901 cgcggtcccg aactgcggtt cggttccgga gatgccctgg tttactgggt cgaaaagatg
     3961 ggcaaatccg agggcctggg ggagaagctc gccatgggca gccacagact ctgcgaagcc
     4021 tacgggcatc cggagttctc catcacggta aagaaacagg aactgcccgc ctatgacccg
     4081 cgtggtatcc agggtatcgg cctgacctac gccgtcagta accgtggggc ctgccacgtg
     4141 aggggttacc tgatatcccc tgaagttctt ggtataccgg agaaactgga cccgtgggag
     4201 atcgacggaa agccgatgtg gggcaagata ttccacgacc tcacggccgc catcgactca
     4261 tcgggtatgt gcctcttcac atccttcgcc cttggagcgg aggattacgc cgcaatgctc
     4321 aagcacggcg ccggttttga tatctgtccg gaatcactcc tcaagatcgg tgatcgcatc
     4381 tggacactgg aacgtctttt caacctcagg gaggggctcg atcctgcgaa ggacgacact
     4441 cttccagtcc ggcttctcaa cgaaccgatc cccgacgggc cggcaaaagg aaaactgagc
     4501 aggataaagg aaatgatccc ggagtactac aagatccgcg gctgggacgc aaaaggggtc
     4561 ccgaccaaag atacgcttgt gatgcttggg ctgaactagg atagtacagg tatgacaaag
     4621 gtctccttct tcggcatggt ccgcgacatt atcggagaga aggaaacgca ggtagagggt
     4681 ggctattctg tcgaaagcct tctggaagca ctctctctga agtatggcct tactttcggg
     4741 gaaaaagtcc tgaagatgcc cgaacgcgac atggacctga tcattcttct taacggcagg
     4801 catatagagc atgtaggagg tctgtcggct ccgttgaaag aaggagacca tctggcggtt
     4861 ttccctctcg ttggaggcgg gtaaccgttc cgtgtgaaga tcgtgttcca ctatgatccg
     4921 gggggcccct ctgcgataat gcggggggcc cgtttatttc gtccctgggg tgatcttgct
     4981 tcattcggga ggaggattat ctcttgagga gagagaagat cgagatgcgc ggcgtatccc
     5041 tggatgagat ctcgtcccat ttccgcgaaa cagggtggca ggaggtttcg cctggcctct
     5101 ggcagaggga ttgtgacagc gtggaagcgg gacttcagga ggaggtctcc ctgggatcca
     5161 tccggctccc ttcggttaca cttgtcttca ggctcaggag gggagatcat gaaaaagtgt
     5221 tatccgcctt ccgttccaga ttcctgaagg caggcggcta gtccggggag gctgatagcg
     5281 tggtcacaag aaaaattgag ctcaacacag gtattgtggc tcccggggta gggtttggaa
     5341 gttctgcgat tccgacggaa cctcaccgca ggataatggg gcactacgca tcgccgcttc
     5401 ttctgggtcc agatccctcg caggatctgc ttgagatggt aatgcacatg ttctccgatg
     5461 aagaggccca ggttgcgcag tatcttcctc ccctgaggcc gcggacggcg gaggcggtct
     5521 ccgccagggc gggacacact cttgatgaga cttcccgcat actcgactcc cttgcacata
     5581 aaaaacgcgt catcctggcc agcggaagcc cccgcaaata cacgatactg cccatagttc
     5641 ccgggacctt cgaaatggcc ctcatgaccc cggacctttc gacgaggaca aggtggcatg
     5701 ccgattttgc gaggctgttc gaaaagatat gggacgaggg gtacatcgtc cgctacaccc
     5761 gttcgatacg ccccggcatc aggtatctcc cggttcacaa ttctgtcccg tcactgctga
     5821 aggcctgccc ctcggagcgt atggaggaga tggtgcgccc ctacagcgac tttgccgtcg
     5881 gtatgtgcca gtgccgaatg gcgatgaccc ttgtcgacaa gggttgcggc cgtccgatgg
     5941 agaactgtgt cgtcatgggt cccggcgcgc agaaattcat cgacggaggt ctgatgcgcc
     6001 gcaccgacgc agccgaggtc ctggcgatca aacgccatgc ggaagagaac ggatgcgtga
     6061 gctggtccat gagcgacgta gggggagttc cgcgcggaaa ttcgagctgt tcctgctgcg
     6121 gatgctgctg tcacgccatg aggagcataa gccagttcaa cttccccgca atgataagcc
     6181 ccccggtttt catgccgatc ctggatcccg caaaatgtgt caattgcggc gcatgtgttg
     6241 ttgcctgccc gatgaatgcc tggtcttccg acggaagttc cctgctcttc gacccggcgc
     6301 ggtgcatagg atgcggacta tgtgtcgtcg cctgcaaaaa gggggcgatg accctgggag
     6361 agtccgggaa caggctgaaa aatgcccccg gatggttcgg ctttttcctc gacaacgctc
     6421 cgggctatct gttgaacgcc ttccgcgtct gggcacggag acacctttaa acctgattct
     6481 cctcatacag aactgaagcg tatatacttt ttacagggag gtggatcaag tgaaggattt
     6541 cgaaaagatg ggctctttct acctgggcaa acgagtggac gacgcctcgg gatcgatgac
     6601 aggggagatg atcctctacg atgccaagga cctcacgacc catggcatca tcataggaat
     6661 gaccggaagc ggaaagacag gccttgccct ttctctcatc gaggaggccc tgatggacaa
     6721 catccccgtc atagccatcg atcccaaggg agacatccca aaccttcttc tgacctttcc
     6781 gcagttcaga cccgaggatt tccgcccatg gataagcgag caggaagcgg cacgaaaggg
     6841 tctttccccc gacgaatttt ccgcgaagac ggcctcggac tggaagaacg gcctcgccga
     6901 gtggggcatt gacggcgccc ggatcgagaa gatgagaaac tccgtggaca tgacggtcta
     6961 cactccgggc agttctgccg gcaggatggt gagcatactg cactccttcg atgcaccccc
     7021 cgaagcgata aggtccgatg gggacctctt cagggagagg gtccagacca ccgcatcgag
     7081 ccttctggcc ctcatgggga tcgatgccga ccccctcacg agccgggagc acatcctcat
     7141 ctcgaagata ctcgaggaaa agtggaacgc cggccagtcg ctggacattc cttccctgat
     7201 cgaccttatc agggtccccc ctttcaaggg gatgggggtc ctcgacctgg agaccttcta
     7261 cccctcgtct tcgaggatgg atcttgtcct gcggcttaac aacctcgtgg cggcgccttc
     7321 cttcgggcca tggatggagg gagagcccct cgatgcggcg aaattcctct atacggccga
     7381 tgggaggccc agggcctcga tattcaccat aagccacctt tcggagaggg agaggatgtt
     7441 cttcgtctcc ctgctcctca acgagatact gggttgggta aggacacagc ccgggacgtc
     7501 gagcctgcgg gccattctct atatggacga gatattcggc ttcttcccgc cggtgcagaa
     7561 ccccccgtcg aagaccgtcc tgctgaccct tctcaagcag gcccgggcct acggactcgg
     7621 ggtgctgctc gctacccaga atcccgtcga cctcgactac aagggtatat cgaacacagg
     7681 gacgtggttc ataggacgcc tccagacgga acgggacaag gcccggatcc tggaaggtct
     7741 caagggggtt tcggaaaagg gattcccgac ccaggaactt gacaggatga tatcagggct
     7801 tgggaagcgc gtcttccttc tgcacaacgt acacgaggac gaaccggtcc ttttccagac
     7861 gagatgggcc atgtcctatc tggccggacc cgttacaagg gagcagatcc ggatgatcga
     7921 ggggcctcat gccgcagttc cgacacgggg aaggggagag gccagggctc cttcggtgtc
     7981 cgccgctcca agaccagtcc aggagatcat tccctcggac attgtctctg ttttccttcc
     8041 ggtctccccc gggatcgacg cggaatatgc ccccttcctg ggagggttcg cgaggataca
     8101 cttcagggat tcgaagctcg gggtcgccga gacgaagatg gttgccttcg cgactgaaat
     8161 gagcgaaggg cctctcaccg tcgactggga gagcgccttt gagatatccg tcgatcccgc
     8221 cgtcctttca caggggatgc ccccttcggt aatgtccccg gttccgacag caggacttga
     8281 ttcgaagtcc tacaccaagt gggggagaga ttttcttaag tggcttccga acaacagttt
     8341 gctgacggta atgacatgcc catcgatgaa agaggcggca aggcccgggg aatcgaaagc
     8401 cgatttcgcg gcgcgcctgg cgctttcacg ccgggagcaa cgggatgctg ccgtcgagaa
     8461 gatccgcaaa aaatatgcct cgaagctgac gactttgcag aaccgcctga tgagggcgga
     8521 gcaggccgtt gagagggaag ctgagcaggc caaaagcagg aagatggata cggcgatatc
     8581 ctttgggaca gccatcgccg gggcctttct cggcaggaag gccagcgtct attccgcgtc
     8641 tcgaatggga acggcggtct caaaggccgc gaggctgagc aaggagtccg gtgacgtagc
     8701 aagggcgaag gagaccgtgg agtctgtaag gctcgaaata gcaggaatgg agcgccagct
     8761 cgaggatgag atcaacgcca ccggtttcgg ggatattctc gagacgggaa tagaggagag
     8821 tttcatccgc ccaaaggcat ctgatatgtc ggttgtcctc ttcggtctcc tgtggcttcc
     8881 ttacgcaagg aatccgcagg ggggtctttc gcccctgtgg ggagactgaa tgaaacggga
     8941 agatgttcac agtcagtgtt atcatgaaac gccaggtaac aagttcgaag aaaaggaagg
     9001 agaatgaaga tcgtgaggaa aacagttgca acagacaaag ctccggcagc gataggtccc
     9061 tatagccagg ctgtatgggc caacgatatt ctcttcgtat cggggcagat acccatcgat
     9121 ccgaaaagcg gggaaatgag cgggaaaact gcggctgaac aggctgaaca ggttctgaaa
     9181 aatgttggag ccatattgga atcccaggga ctttcatttg cgagtgtggt caaggcgacg
     9241 gttttcgcaa aggacatgaa tgatttcaag gccgtaaacg atgtttacgc cagttatttt
     9301 accgctgacc cccctgcgag ggcctttgta gaggtggcaa ggcttcctaa ggatgccctg
     9361 gtcgagatag aggtcatcgc cgtaaaaggt tgatgcattg acaaacagac caaaaatacg
     9421 tactgtttta tcagccggtt aaatagacgg cggattctgc tttctgggag gcattatatc
     9481 tatgcaggga acagtcaaat ggttcaacga ggccaaaggg tacggtttca tcacccccga
     9541 cgagggcaac gatgtgttcg tgcacttcag cgcgatccag ggcgatggat tcaagacgct
     9601 tgccgaagga cagcgcgttg agttcgaggt agtccagggg gacaagggcc cccaggcggc
     9661 caatgtagag aagatctcct gaacgtccgg gatcttttct tcacggggag atagttttgc
     9721 ggcgactcct tccccgaagg ggttcgccgc ttttttcggg aatgatcacc tgttcgcgga
     9781 ttgagccgga gccttgcttt atctcacggc ttgatgcgat aatcccctgt aatgacaaac
     9841 ttctggaaaa ggagggaagg atttgaataa gagcgaactg atccaggcgg ttgccaagtc
     9901 ggcgggactg agcaatagca aggcgaccca ggtggtttcg gcggtcatcg acgagatcgg
     9961 cgcagcgttg gccggtgggg acaaggtaca ggttgccgga ttcggcacct tcgaggttcg
    10021 gcagcgcgcg gcgagagagg cccgtaatcc gcaggatccc acgaagaagg tcaaggttcc
    10081 ggcaagaaag gttcccgctt tccgcgccgg caaggccctg aaagacaagg tctgctagag
    10141 tttcgtcttt ttgatgtaat atttgccgcc ccgcaagggg cggttttttt ttaagactcg
    10201 tgaagc
//
LOCUS       DAOTIQ010000061         9929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_8100_length_9929_cov_6.384748, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000061 DAOTIQ010000000
VERSION     DAOTIQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8100_length_9929_cov_6.384748"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="P5541_07605"
     CDS             <1..420
                     /locus_tag="P5541_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV71888.1"
                     /translation="TAVLEKMFSDPEDLSLVWPVAHWPFDHGQWQQVLDQGKGAISFL
                     VYEEKTLIGHAALDRAENPATRMVRFLYIAPEHRSRGAGQRMIALLEEYARERLGAKR
                     LELKVRSFNGRALRCYEKCGFAEFFREDTLVLMGKDI"
     gene            complement(861..1817)
                     /locus_tag="P5541_07610"
     CDS             complement(861..1817)
                     /locus_tag="P5541_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV71889.1"
                     /translation="MIKVDEGIVRVDNRLSRGSQIRHFAGLAACMVFLVMAFGAVEAR
                     AEWPHVVVSKDGTPISHEVSGTGEPTLVFVHGWSCDARYWRAQVPYYSKNHRVVVLDL
                     AGHGHSGTSRTRYTMRAFGEDVRAVTEEACEGKVILIGHSMGGSVIAEAARLMPDRVI
                     GLIGIDTLENVEYPLTPTELEEMLAPLKEDFRTGASQFAGQMISPDTDPQIRDWILAD
                     MSSAPPAVALSAMKEMMSQYITGEASKIFEQIRIPVVTVNGDLWPIDYEANRRHMFSF
                     DAIVLKNADHFLMMDEPEEFNAALGKAIDMVLKKSAEIHRAD"
     gene            complement(1998..2576)
                     /locus_tag="P5541_07615"
     CDS             complement(1998..2576)
                     /locus_tag="P5541_07615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV71890.1"
                     /translation="MNPHVRKFQSIAPFYGLFFKPQVLYYSRIIEKHISELNLAGCET
                     ILDLGSGTGAFARCWQDLGYSVTAVDASPAMVRLCLRNGLKCLEQDVLRGLNFPDNSF
                     DVVVGAYVAHGFPFADREKLYRESARVSKDLVLFHDFGGKPSFSVSVIEKMEGSCYKE
                     FISRAPGEMKEFFRRVDVLSFSSWNRWYICRP"
     gene            2842..3828
                     /locus_tag="P5541_07620"
     CDS             2842..3828
                     /locus_tag="P5541_07620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRV71891.1"
                     /translation="MYTYGQNQGLAPNPKILGSVLEAIGNTKIVNCSRLASHWGISGT
                     ILAKLEYLNPGFSKKDRVGLHMVLEAEEKGLISTGDTIVELTSGNTGTGLALACGIKG
                     YRFVAVMSEGNSMERARMMRALGAEVVLVPQTPGSIPGQVSGQDLELVNDEAQRIVKE
                     RGAFRADQFAMEGNVLGERVSGEEIWKDTGGQVDFFVDMAGSGGSFTGISSVLKEHNP
                     AIRCYLGEPAEAAHFGSPGGSGSHRIQGCGYSRNLPLLDRSLVDGFISITDEEAVEGA
                     RALARHEGIFGGFSSGAHAAAVKKMLEGPERGKTGVFLVCDSGLKYLSTDLY"
     gene            3864..>4540
                     /locus_tag="P5541_07625"
     CDS             3864..>4540
                     /locus_tag="P5541_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV71892.1"
                     /translation="MKKDMSKDPLFVDEKFPPDMHDLFIEDRLLGLIYLADGKGPHGT
                     VLFLHGFPGNEKNLDIAQMLRRGGYNVMVFHYSGSWGSRGSYSFSQNYRDLQAVRKAI
                     ADEAFAREHRIDRENVYLAGHSVGGFLTLLAARDGMDFKGFAALAPYNLGKQAERIHG
                     GDEEAHRETLDLFEGGLRPLSGATAESLMADIMENRASWDITGNPEAYADRKMLLVMA
                     AGDICAWP"
     assembly_gap    4541..4640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4641..6377)
                     /gene="glgP"
                     /locus_tag="P5541_07630"
     CDS             complement(4641..6377)
                     /gene="glgP"
                     /locus_tag="P5541_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRV71893.1"
                     /translation="MKRMVYGNDIGHSLRYMMEQDPAFRMVAYFSMEIGLKNSIPTYS
                     GGLGILAGDILKSAADLGVPVAGVTLLYRKGYFRQVIEECSQKALPVEWDPEKELTLL
                     PNEVTVLIESREVKVRAWCYEIEGKSGFTVPVYFLDTDVEGNIPKDRELTWYLYGGDQ
                     RYRLCQEIILGSGGLRMLRDLGFANIDNYHLNEGHAAFLALELIREMGYERYDKVREK
                     GIFTTHTPVPAGHDRFSWDLVKSVMNPAMAKRLGRMMPTNDVSMTEIALKYSRYVNGV
                     SRKHTEVSRAMFKKDDVDCITNGIHSSTWVSPEMSALFAKHIPGWDNAPERLVQAMTI
                     PVDEIRQAHVAAKKRLLEYVARKTGTNLDPDCLTIGFARRVAQYKRADLIFRDIPRLA
                     KAAGGKVQFVFAGKAHPHDEPAREILRKLLCEAENLTGANLPVVYLEDYGMDLGALLV
                     QGCDLWLNNPIRPREASGTSGMKCALNGVPNFSVLDGWWIEGCVEGVTGWSIGPEPTE
                     TIDDHYDESVDLDDLLEKLEKVIIPAFYDRPEEWGKVMRGAIALNGSYFNTHRVVREY
                     CERAYGIRMRGL"
     gene            complement(6421..8004)
                     /gene="malQ"
                     /locus_tag="P5541_07635"
     CDS             complement(6421..8004)
                     /gene="malQ"
                     /locus_tag="P5541_07635"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRV71894.1"
                     /translation="MERQGRLSGKIGPMKTSTLFEKKNIRTSGILMHISSLPSPWGTG
                     DLGPGADGFVDFLVNAGQGLWQMLPLSPTDPGQANSPYSGLSAFAGNPLFISPEILAD
                     DGLLDPSDIKAPGGFPAASADYVCSARAREPLFRKAFAAMRSMPDPGFSSFCEKNSFW
                     LDDFALFSALKTTRNGSPWYEWPVPLRDREGLALEEARRELAGEMSYQKFLQYVFFRQ
                     WNRLRNICARRGIALVGDIPVYVSHDSADVWSNRRLFDLDGEGRPRNVAGVPPDYFSP
                     LGQRWGNPVYRWDILEADGFGWWLGRLEHALFLFDLVRVDHFRGLIKYWSIPAKEKTA
                     VKGRWVDASPDAFFAKVRETFPEMPFIAENLGVITPDVTAWVERLGVPGMLVLQFAFG
                     DDFEKNPYAPANHIENACVYTGTHDNDTSRGWFETVATTGVKRQIESLAGHEVTPESV
                     SMDMISIAMRSKARMAIIPMQDILGLGTEGRLNTPGTASGNWIWRVDERGLENVPVSF
                     LADLALQNGRLAPSPPGVR"
     gene            8089..8802
                     /locus_tag="P5541_07640"
     CDS             8089..8802
                     /locus_tag="P5541_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV71895.1"
                     /translation="MPEEKATWRLDKVFKTYDMGEVKVEALRESSLSILDGDLLVILG
                     PSGSGKSTLLNLLGGMDVPSGGDVWFRDENLATAKDRELTRYRRNVIGFVFQFFNLIP
                     DLTAFENVAIAADLVESPLPPEVVLEQVGMGDRMDHFPSQMSGGEQQRVAIARAIVKN
                     PLLLLCDEPTGSLDSTTGRLILALLEEINRKYGTTVVIVTHNAAIADMAGRVVKMSSG
                     RIIEEKVNRKPLSPERIDW"
     gene            8802..>9929
                     /locus_tag="P5541_07645"
     CDS             8802..>9929
                     /locus_tag="P5541_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV71896.1"
                     /translation="MPVLAKKLLRTIGSTFGQFAASAVVVAVGIVAFVSMTAVSDNLI
                     RSRDAFYRETDFAEHFFHVVKAPEGVLARVEAVEGVLRATVRLQKDVPVLRDDGMRTS
                     LRLTGYPLPEERELSRIRVMEGRLFEKYPEGGAVEILLDPLFAKAHGLGQGDTVPVGA
                     EGRRKVLTVTGTAAGPEFVYAVKGAGSLLEDPSSFGIAVIPQNQLQQLLEMKGYVNQV
                     LIRFTPGTDTAKVVREIEKILEPYGNLTNYPRKDQISEAILRGELNQLKAFARFLPSI
                     FLGVAVLMQFVFIGRMVQVQRGQIGVLKAIGYGNGSLLALYGGYALAATACGAAAGTL
                     LGYILAQLLTKVYSAFFNLPRLEEALHFRTAWVVLSATLAVG"
BASE COUNT         2178 a         2763 c         2758 g         2130 t
ORIGIN      
        1 acggcagtcc tggaaaagat gttcagcgac ccggaggatc tcagccttgt ctggcccgtt
       61 gcgcattggc ccttcgatca cgggcagtgg cagcaggtcc tcgaccaggg caaaggggcg
      121 atctccttcc tggtttatga agagaaaact cttatcgggc acgcggcttt ggacagggcg
      181 gaaaaccccg caacgcgaat ggtccgtttc ctctacatcg ccccggagca tcggagcagg
      241 ggcgcggggc agcggatgat agcccttctg gaggagtacg ccagggagag gctgggagcc
      301 aagcggctgg aactgaaggt ccgcagcttc aacggaaggg ccttgaggtg ttacgagaaa
      361 tgcggcttcg ctgaattctt cagggaagac accctggttc tgatgggaaa ggatatataa
      421 atagtcccga gtatcttcag gaagcaggag acagacgtca ggaggcaaaa tcgaaattat
      481 cttcgcaagc cccagggact atccaaacag gctcagttgc cattcgtaaa accgcctcag
      541 gacttcgcgc tcggggttac aggaaatgga acgattggcc tacggctgag gtctttcgtt
      601 gaagcccttg taaccgtgct tttctgcgtt tcccaaaagg aattgctgcg gcatgacccc
      661 ggtaccccac catgagttga aggccataag aaccctgaga atgctttcag gggcttgtaa
      721 cagacatggc tcccacactg cagtgcagtt gagatgatgt caaaatgcat ttttcgttga
      781 tttattgcca agtataaaat gcattattgt ggtcaaatat gcaagtataa gttcacaagg
      841 cgtgtattgg atctgctgga tcagtctgcc cggtgtattt ccgccgattt ttttaaaacc
      901 atgtcgatgg cttttcccag cgccgcgttg aactcttccg gctcgtccat catcaggaaa
      961 tgatcggcat tcttcaggac gatggcatcg aaggagaaca tgtgccgccg gttcgcttca
     1021 tagtcgatgg gccacaggtc tccgttcact gtcacgaccg gaatgcggat ctgttcgaat
     1081 atcttcgacg cctctcccgt aatgtactgc gacatcattt ccttcatcgc actcagggcg
     1141 acggctggcg gcgctgagga catatcagcg aggatccagt cccggatctg cgggtcggtg
     1201 tcgggcgaga tcatctgtcc tgcgaactga cttgctccgg ttcgaaaatc ctccttcaaa
     1261 ggggcaagca tctcttcgag ctccgtggga gtaagcggat attccacgtt ttcaagagta
     1321 tcaatcccta tcaggccgat cacacgatcc ggcatcagcc gggcagcctc ggcaatgacc
     1381 gaccccccca tggaatggcc tatcagtatt actttcccct cgcaggcttc ttccgttacg
     1441 gccctgacat cctctccgaa agcgcgcatc gtatatctcg tacgtgaagt tcccgagtgg
     1501 ccgtgccccg cgagatcgag aacgacgacc cgatggtttt tggaataata cggcacctgg
     1561 gcacgccagt agcgggcatc gcagctccag ccgtgaacaa acaccagggt cggctctccg
     1621 gtaccggaga cttcatgcga gatcggggta ccgtctttgg acacgacgac atggggccat
     1681 tccgcccgtg cctccaccgc gccgaatgcc atgaccagaa aaaccatgca ggcggccaga
     1741 ccagcaaagt gtctaatttg tgatccccgg ctaagcctgt tatccacccg aacgatacct
     1801 tcatcaacct tgatcattca aatcctctcc tgccagaagt ttttggcccc tgtcacaaag
     1861 atcccatttt ccccttcgtc gggaaccgca tcagttgttc cgtcggcggg tacatgagcc
     1921 aatccgattc aaaaattatt tttattcttt tcgttaataa cacacaacgc gatagtgcaa
     1981 ggactccttc ctgccactta cggtcggcag atgtaccagc gattccagga ggaaaacgac
     2041 aaaacatcca ccctcctgaa aaattctttc atctctcccg gagcgcggga aataaattct
     2101 ttgtagcagc tcccctccat tttttctatc actgatacgg aaaagcttgg cttcccgccg
     2161 aaatcgtgaa acagaaccag gtctttggag actctggccg attccctgta gagtttttcc
     2221 ctatcggcaa agggaaaccc gtgagctaca taagcaccga caacgacatc aaaggaatta
     2281 tccgggaaat tgagacccct caaaacatcc tgttccaggc atttaagccc gtttctcaag
     2341 cataaccgga ccatagccgg agatgcatct acagccgtca cactgtagcc aagatcctgc
     2401 cagcaacggg caaacgcccc tgttccgctg cccaggtcaa gaatggtttc acatcctgca
     2461 agattcagtt cggatatgtg tttttcgata atccgtgagt aatagagcac ctgcggtttg
     2521 aagaaaagcc cgtaaaatgg cgcgatagac tggaacttcc tcacatgagg attcatcaaa
     2581 aaccttcttt ccagggagaa aggctcgacg tccttcgttg cgatcattct acacaagtcc
     2641 gggtgcagat ggaaaccggc aatgctctga cctgcatgcc gggccatttt caaaatgatt
     2701 aatacggtcc attgataaca taaaagattg tcgagcaata agcaggcccg aggtaaaata
     2761 gtgctataaa tgaggagcac tctccggtaa cgcgatctcg cttcagtcgg ttttcaatca
     2821 aagcagaaag gcaggttcaa catgtataca tacggtcaaa atcaaggtct ggccccaaac
     2881 ccaaagatac tgggaagcgt tctcgaggca ataggtaaca cgaagatcgt caactgcagc
     2941 aggcttgcca gccactgggg gatttcgggt accatccttg caaaactgga gtacctgaac
     3001 cccggtttca gcaaaaagga ccgcgttggc ctgcacatgg ttctggaggc ggaggagaag
     3061 gggctgatct ccactggcga caccatagtg gaactgacca gcggcaacac cgggaccggc
     3121 cttgcccttg cctgtggaat aaagggttac aggttcgtgg cagtgatgtc cgagggcaat
     3181 tccatggaac gggccaggat gatgagggcc ctgggagccg aggtagtgct ggttccccag
     3241 acaccgggat cgatcccggg ccaggtctcg ggccaggacc ttgaactcgt aaacgatgaa
     3301 gcccaacgga tcgtgaagga acgtggtgct ttcagggcag atcagttcgc catggagggc
     3361 aatgtcctgg gagaacgggt ctccggtgag gaaatttgga aagacacggg gggacaggtg
     3421 gatttctttg tagatatggc aggctcagga ggctccttca ccggaatttc cagcgttctg
     3481 aaggaacaca accccgcgat acgctgctac ctgggggaac ctgccgaggc cgcccatttc
     3541 ggcagtccgg gaggcagcgg gagccacagg attcagggat gcggctactc aaggaatctt
     3601 cccctgctcg accgatccct cgttgatggc tttatatcca ttaccgacga ggaagcggtg
     3661 gagggagccc gcgcccttgc aaggcatgag ggaatattcg gagggttttc ctcgggagcc
     3721 cacgccgcag ccgtgaaaaa aatgcttgaa ggccccgaga gagggaagac cggcgttttc
     3781 ctcgtatgcg attcggggct caagtatctg agcaccgatc tttactgatg caacagatca
     3841 caacttccgg gaggcacaga taaatgaaaa aggacatgag caaggacccc ttatttgtgg
     3901 atgagaaatt cccgccagac atgcacgacc tctttatcga ggaccgcctt ttgggtctga
     3961 tctacctggc cgacgggaag ggcccccacg gaaccgttct gttccttcac ggttttcccg
     4021 gcaacgagaa gaacctggac attgcccaga tgttgaggcg cggcggatac aacgtgatgg
     4081 tctttcacta ctccggttcc tggggcagca ggggctccta ctcgttcagc cagaattaca
     4141 gggatctcca ggctgtacga aaggcaatag ccgacgaagc cttcgcccgg gaacacagga
     4201 tcgacaggga gaatgtttac ctggccgggc acagtgttgg aggatttttg accctcctgg
     4261 cagcaaggga tgggatggat tttaagggct tcgcggctct cgccccatac aaccttggta
     4321 aacaggccga gaggatccat gggggtgacg aagaggcgca cagagagact ctggaccttt
     4381 tcgagggagg tttgagacct ctgagcggcg cgaccgccga gtctctgatg gccgatatta
     4441 tggaaaatcg cgccagttgg gacataaccg gcaaccccga ggcctatgcg gacaggaaga
     4501 tgctcctggt catggccgcc ggagacattt gcgcctggcc nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn ctagagtccc ctcatccgga ttccgtaggc ccgttcgcag
     4681 tattcgcgga cgaccctgtg ggtgttgaag taacttccgt taagcgctat ggcgccgcgc
     4741 atgactttgc cccattcttc gggcctgtcg tagaaggctg gaatgatcac tttctcaagt
     4801 ttttcgagca ggtcatcaag gtcgacggat tcgtcgtaat ggtcatctat tgtttccgta
     4861 ggttccggcc cgatgctcca ccccgtgacg ccctccacgc acccttctat ccaccagccg
     4921 tcaaggaccg agaaattggg gacaccattg agggcgcact tcatgccgct tgtccccgat
     4981 gcctccctgg ggcggatggg attgttcagc cagagatcgc agccctggac cagcagggct
     5041 ccaaggtcca ttccgtaatc ctcaaggtat acgacgggca gattcgcacc tgtcagattt
     5101 tccgcctcgc agagcaactt ccggagtatc tccctggccg gttcgtcgtg ggggtgggcc
     5161 tttccggcaa agacgaactg caccttgcct ccggcagcct ttgccaaccg tggaatatcc
     5221 cggaatatga gatcggccct cttgtactgg gccaccctcc ttgcaaaacc tatggttagg
     5281 caatctgggt caaggtttgt gccggtcttt ctggccacat attccaaaag tcttttcttt
     5341 gccgcgacgt gggcctgtcg tatctcatca accgggattg tcatggcctg gacaagccgc
     5401 tccggcgcgt tgtcccaccc gggtatatgt tttgcgaaga gggcagacat ttccgggctc
     5461 acccaggtcg aagagtggat cccgttggtt atgcagtcca catcatcctt tttgaacatg
     5521 gccctgctga cctcggtgtg tttgcgggag acaccgttga cgtagcggct gtatttgagg
     5581 gcgatctccg tcatggagac atcgttggtg ggcatcattc tgccaagtcg ttttgccatg
     5641 gccgggttca tgacactttt taccaggtcc caggagaatc tgtcatgccc cgcgggcact
     5701 ggtgtatggg tggtgaagat ccctttttca cggactttgt catatctttc gtaacccatc
     5761 tcgcggataa gttccagggc aaggaaagca gcgtgtccct cattgagatg gtagttgtcg
     5821 atgttggcaa accccagatc gcggagcatg cggagtccgc cggagccaag aattatctcc
     5881 tggcagagac gataacgctg gtcccctccg tacaggtacc aggtcagttc cctgtctttg
     5941 gggatgtttc cctcgacatc ggtatcaaga aagtagaccg gaacagtaaa accgctcttc
     6001 ccctcgatct cgtagcacca cgccctgacc ttgacctccc gggattcgat gagcacagta
     6061 acctcgttgg gaagcagcgt cagttccttt tccggatccc actccacggg caacgctttc
     6121 tgtgaacact cttctatcac ctggcggaaa taacccttcc ggtaaaggag ggtcactcca
     6181 gcgacgggta ctcccaggtc ggcggcactc ttgagtatgt cccccgcaag gatcccgagc
     6241 cctccggaat aggtggggat gctgtttttc agtccgatct ccatcgaaaa atacgccacc
     6301 atacggaatg caggatcctg ctccatcatg tagcgcagtg agtgcccgat atcgttcccg
     6361 tagaccattc ttttcatgct gtcggctcct cctcttgttc tctcttctga ttctttcata
     6421 ttatcgcacg cccgggggcg agggcgccaa cctgccgttc tgcagagcca gatccgccag
     6481 aaatgaaacg gggacattct cgagacccct ttcatcgacc ctccagatcc agttgccaga
     6541 ggcagttccc ggggtgttga gtctgccctc cgtgccaaga ccgagaatgt cctgcatggg
     6601 aattatcgcc atacgggctt tggatctcat ggctatggat atcatgtcca tagagacgct
     6661 ttcgggggtc acttcgtgcc ccgccagcga ttcgatctgc cgcttcaccc ccgttgttgc
     6721 aacggtctcg aaccaccctc tcgaagtatc gttgtcgtgg gtcccggtgt agacgcaggc
     6781 attctcgatg tgattcgccg gggcgtaggg gttcttttcg aaatcgtctc caaaagcaaa
     6841 ctgcaggacc agcattccgg ggaccccgag acgctccacc caggccgtta cgtcgggggt
     6901 tatgactccg aggttctcgg cgatgaaggg catttcggga aaagtctccc ttaccttcgc
     6961 aaaaaaggca tccggtgagg catccaccca ccgacccttc acggcggttt tctcttttgc
     7021 cggaatcgac cagtatttta tgagccccct gaagtgatct acccgaacca gatcgaagag
     7081 gaaaagggca tgttccaggc ggccaagcca ccagccgaaa ccatccgctt cgagaatatc
     7141 ccagcgatag acggggttgc cccacctctg cccgaggggc gagaagtagt cggggggtac
     7201 accggccacg ttacggggac gtccctcccc gtcgaggtcg aagaggcgcc tgttggacca
     7261 gacatctgcg ctgtcgtggc ttacgtagac cggtatgtca cctacaagtg caatgccgcg
     7321 cctggcgcat atatttctga ggcggttcca ctgacggaag aatacgtact gcaggaattt
     7381 ttggtaactc atctcccctg cgagctctct gcgcgcttcc tccaatgcca ggccttcccg
     7441 gtcgcgcaag gggacgggcc attcgtacca gggagaaccg tttcgagttg ttttgagggc
     7501 cgaaaagaga gcaaagtcat cgagccagaa ggagttcttt tcacaaaagg aagagaaacc
     7561 aggatcaggc atggagcgca tcgcagcaaa cgcctttcgg aacaggggct ccctggcccg
     7621 ggcagagcat acgtaatcgg cacttgcggc ggggaaaccc ccaggcgcct tgatatcaga
     7681 gggatcgaga agaccgtcat cagcaagtat ctcggggctg atgaagagag ggtttcctgc
     7741 gaaggcggaa agaccgctgt agggagagtt cgcctgtccc ggatcagtgg gcgaaagggg
     7801 aagcatctgc cagagtccct gccccgcatt gaccagaaag tcgacgaaac cgtcggctcc
     7861 cgggcccagg tcaccagtac cccagggtga gggaagggat gaaatatgca taagtatgcc
     7921 gcttgtgcgg atgtttttct tttcaaaaag ggttgaggtt ttcatagggc cgattttacc
     7981 cgataaccgt ccctgccgtt ccatccggaa gacgtaacct ttcatccttc cttttgggtc
     8041 gccgctttgc tatcgtgtaa aatcacctgg gtagcggggg tgtttttgat gccggaagag
     8101 aaagcgacct ggagactcga caaggttttc aagacatacg atatgggaga ggtcaaggtg
     8161 gaggcccttc gggaatcttc cctctcgatc ctcgacggcg acctgcttgt aatacttggc
     8221 ccgagcgggt ccgggaagag caccctgctc aacctgctgg ggggaatgga tgttccttcc
     8281 ggcggcgacg tctggttccg ggacgagaac ctggcaaccg ccaaagaccg ggaactcacc
     8341 cgataccgcc gcaatgtcat aggtttcgtc ttccagtttt tcaaccttat ccccgacctc
     8401 acagccttcg aaaacgtagc catcgcagcc gatctggttg aatctccgct ccctccggaa
     8461 gtagtcctgg aacaggtggg gatgggagac cggatggacc acttcccgtc gcagatgagc
     8521 gggggggaac agcagcgcgt cgccattgcc cgggccatag tgaagaaccc ccttcttctt
     8581 ctctgcgacg aaccgacagg ctctctggac tccaccaccg gaaggcttat tctggctctt
     8641 cttgaggaga tcaaccggaa atacggcacc acagtcgtta tcgtgaccca caacgccgcc
     8701 atagccgaca tggcgggccg cgtggtaaag atgagcagcg gccgcataat cgaggagaag
     8761 gtcaacagaa aacccctctc ccccgaaagg atcgactggt gatgccggtc ctggcgaaaa
     8821 aactgctgag gacgataggc tccacattcg gccagttcgc agcctcggcc gtggtggttg
     8881 ccgtcgggat agtcgccttc gtttccatga cggcagtctc cgacaacctc atcaggtccc
     8941 gcgacgcctt ttaccgcgag accgatttcg ccgaacattt tttccacgtc gtaaaagccc
     9001 cggagggcgt gctggcacgg gtcgaggcgg tagaaggagt actccgggca accgtaaggc
     9061 tgcagaagga tgtcccggtg ctgcgcgacg acggaatgcg tacatctctc cgcctgactg
     9121 gttaccccct tccagaggaa agggagctca gccgcatcag ggtcatggag gggaggctgt
     9181 ttgaaaagta tccggagggc ggagccgtcg agatccttct ggacccgctt ttcgcaaagg
     9241 cccacgggct tggacagggg gacacggtcc ccgtgggagc tgaggggaga cgcaaggtgc
     9301 tgaccgtcac gggaaccgcg gcgggtcccg aattcgtcta cgccgtaaag ggcgccgggt
     9361 ccctgcttga ggacccgtcc tccttcggga tcgcggtgat cccccagaac cagctgcagc
     9421 agcttctcga aatgaagggc tacgtcaacc aggtcctgat ccgcttcact ccgggtaccg
     9481 acacggcgaa ggtggtcagg gagatcgaaa aaatcctgga accttacggg aacctgacaa
     9541 actacccccg gaaggatcag ataagcgagg ccatcctcag aggagagctg aaccagctca
     9601 aggccttcgc ccggtttctc ccctccatct tcctcggcgt ggccgttctg atgcagttcg
     9661 tgttcatcgg gcggatggtg caggtccagc gggggcagat aggagttctc aaagccatcg
     9721 gctacgggaa cgggtccctg ctggcgcttt acgggggata tgccctggcc gccacggcgt
     9781 gcggagccgc ggcaggcact ctgctggggt atatccttgc ccagcttctc acgaaagtct
     9841 attccgcctt cttcaacctg cccaggctcg aggaggccct tcacttccgg acggcctggg
     9901 tcgtgctctc ggcgaccctc gcggtcggg
//
LOCUS       DAOTIQ010000062         9601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_8459_length_9601_cov_8.076996, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000062 DAOTIQ010000000
VERSION     DAOTIQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8459_length_9601_cov_8.076996"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            497..817
                     /locus_tag="P5541_07650"
     CDS             497..817
                     /locus_tag="P5541_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRV71897.1"
                     /translation="MQEKFENVTLVSKANVYMDGKVTSRTFFTPSGERKTAGIFLPGE
                     YEFGTGDAEIMDVVQGSATVLLPGEADWKTFGPGESFNIPKNSSFKLKIDGVFDYICS
                     YIKE"
     gene            complement(850..1086)
                     /locus_tag="P5541_07655"
     CDS             complement(850..1086)
                     /locus_tag="P5541_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71898.1"
                     /translation="MERSLLQAAIGAIFMGLGAGFLMYSYADKITGKKKKNIRREEYY
                     DPSKDDRIDNARLVKAISAGFFMGVMFFIYFSNR"
     gene            1202..1990
                     /locus_tag="P5541_07660"
     CDS             1202..1990
                     /locus_tag="P5541_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71899.1"
                     /translation="MNKFVLLLAVFFVVFSPASAFSSEWERVGDAAWAEVSSIEEFET
                     VLEEMLGSFEEHAVIRLPLKRCLEEDEIQGMLQSIYYRRDLVAASMARMEATIRYASG
                     MNTVEFLFKWIDSRQQRDEVREMVPRILSSILAPGMSERQILEAIHDHVVLSVVYDDS
                     RMGRGAWEAIHGRAVCQGYALLTEALLSEAGFENRVVLNSDHAWNLVFTQGTWRHIDT
                     AFDDPIIIGGGKPEGISREAFLKTDAEMRALGHNWDEKRYPKAR"
     gene            complement(2025..3296)
                     /locus_tag="P5541_07665"
     CDS             complement(2025..3296)
                     /locus_tag="P5541_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71900.1"
                     /translation="MSPMRPENKNRRMINAWCLYDAGNSAFATTIMAAMLPVYFREVA
                     AAGLPPNMPSALWGYTSSIALLSGALIAPILGSMADVGEAKKKFLAFFTLLGVFSTAL
                     LFFVEEGDWFLALLLYGGGSLGFSLSMIFYDALLPHIAPPGQMDIVSSRGYAWGYLGG
                     GTLLAANIAAAAIIPGTLGVRVSFLSVAIWWSAFTIPLMLLIPEPPSALPRPLNRNEV
                     MTRGFSQLAQTFRKMRNYRDMLIFLGAYWLYNDGVGTIMKMAAIFGSEKGIGMMHLVG
                     ALLATQFIGAPFAILFGRLSVKLGGKRTITIGLVWYMMVTIGAFFLETATHFWILAIA
                     VGMAQGGVQAISRSTYGMMVPKAQTGEFFAFYDIFSKFSGVAGPTIFAVLTQLTGSTR
                     LGITSLIVLFAAGIFLLQKVDVARGIERSKE"
     gene            3432..>4513
                     /locus_tag="P5541_07670"
     CDS             3432..>4513
                     /locus_tag="P5541_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRV71901.1"
                     /translation="MKIRTLDGPRLHRGFTAGARNIQIRQETLNRMNVFPVPDGDTGS
                     NLAATVQSVSEGAVISRSASDTSASMADAALLGARGNSGLIFAQFLYGFAQESNGKDE
                     INARDFGRAVAGAIPYAREALSNPVEGTILTVMEDWASEVKSITRQSNDFALLFPGSL
                     EVARRSLQETPKKLAVLARAGVVDAGAQGFVNFLEGIVSFLESGNLRNYSGQMELPVI
                     SHVHDDFSEGEPSHRFCTEALLCGEGLDKAQIRKEMQPFGDSIIVGGHGSKVRVHIHT
                     DTPDRLFFSMRKHGALKQQKADDMKRQVDVCRRRLHSVAILTDSTCDLPAEFMDENQI
                     HTVPLRLAFGDSVFIDKVTISPDQFYT"
     assembly_gap    4514..4613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4614..5003
                     /locus_tag="P5541_07675"
     CDS             <4614..5003
                     /locus_tag="P5541_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRV71902.1"
                     /translation="GAKVFVSLKTLKYMVRGGRLSPVKGFLAEVLNLKPIISVDEEGK
                     AFSFGQTRGWDANIRKIEKVISEMQGQGRIWNYCVVHAHSHESAELVAAGLKRIVGKA
                     PAYTMDICPVLGTHSGIGSVAVGILME"
     gene            5011..5334
                     /locus_tag="P5541_07680"
     CDS             5011..5334
                     /locus_tag="P5541_07680"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HRV71903.1"
                     /translation="MSKKKTGEKNAEMKKDTLERLAEVMERSKVLENIEMILDRRKLL
                     WNNFLAGLARGLGFGVGLTVLAAVFIYILVVILKSIVALNIPLIGTYIADLVEIVQEQ
                     VSNHR"
     gene            5369..6034
                     /locus_tag="P5541_07685"
     CDS             5369..6034
                     /locus_tag="P5541_07685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV71904.1"
                     /translation="MSRKIEREGTISTIGKKSVIPKVFMAVAVLFALASGVYIAVDHG
                     KTSAEITGNGSEIPPAAVEGPSKVPPLADPGIVAPFVEEHGLSGKFWIMIDKSDFLLT
                     TWEGDRFLSGYTIATGENPGDKERVGDRRTPEGVFSVKSIHDSRAWVHDFGDGKGPVE
                     HAYGPWFIRLETGWKGIGIHGTHDPSTLGKMVTEGCIRMNNEALLEIVERAVPGTVVL
                     IEP"
     gene            6058..7368
                     /locus_tag="P5541_07690"
     CDS             6058..7368
                     /locus_tag="P5541_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV71905.1"
                     /translation="MKDRKVRVFSLVLAVPLFVAAFWIFSAEVGATDDPAGKLFQALN
                     WVSESPDRAWARVSLGDVYVSLGRHEDAIKAYKTALRFEPDNAFAMNGMGYAWLGLGK
                     PEKALQFFVDAAAADPSKADLKSLGYTWMALGKNAEALDTFNSVLAKEPENTEILNAM
                     GQASYSMGDFAGAVKYFETAISVDPLKGDYASLGYSYSALGMEDRAKESYSEGVRLRP
                     RDQESLNALGFSLFSRGRLEEAREIFYESLDIDPDNVYVLNGLGYVYSAGKDHEKAIE
                     FLEKAARADQSQADYVTTGYEYAALERHREAEAAFRRAISRDPSSAAEAWYGLGYTLI
                     AMGSNVEAMDAFRRSYMLMPEPRTLNSLGYAYMVVRNYPNAIEAFNRALGIDPEDAQA
                     RFNLALAMLDLDRRAEARKYYGELQKIDEELAAELKSIMDEAWK"
     gene            7402..8079
                     /locus_tag="P5541_07695"
     CDS             7402..8079
                     /locus_tag="P5541_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HRV71906.1"
                     /translation="MKYEIIGEGAYPAVKLALAEGDVVTAEAGAMASMDGSVEIQTQM
                     KGGLLGGLKRSMLGGESFFMNHFTSRADGGELVLAPTLPGAIRVREMKGETIFLQSGS
                     YLGSFGDVEIDTKWKGFKGVFAGEGFFMLRVTGSGTLFFNSFGAMYDVQVSGSPYKVD
                     TGNIVMFEEGLDYRVASVGGLKSLVLSGEGLVAEFNGHGLLVMQTRSIPGFIGWITGK
                     MPKKSGN"
     gene            8133..9326
                     /locus_tag="P5541_07700"
     CDS             8133..9326
                     /locus_tag="P5541_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV71907.1"
                     /translation="MQPWDDLYSEVGNELTPSPIRELVKYMKKPGMISLAGGNPDPEI
                     FPVEEFQEAASIITERGKEILQYGATGGYEPLKEFISGWMAPRMGRITALDEMCITTG
                     SQQVMDLFAAVMLDPGDVVIVEEPTYPGATHALKNRKARFITVPCDKDGMQVELIPEI
                     LEQARQGRDRVKFIYSIVNFQNPSGYTLSAPRRKKLLEIAVEYGIPVFEDDPYGHLRY
                     DGPHEPTIFSMDNDDMVIYACSFSKILAPGTRVAWVTGPKEVINKMVMVKQGSDLCTS
                     VLAQGLVYEYCRLGHLESFLPRIQAHYKKKRDAMESAFREYLPSSAIFSTPAGGFFYW
                     LTLPGTNTRDLFFKAIERNVSFVTGDAFYPTGGGEEELRACFSYARAEDFPEAARRLA
                     QAISS"
     gene            9346..>9601
                     /locus_tag="P5541_07705"
     CDS             9346..>9601
                     /locus_tag="P5541_07705"
                     /inference="COORDINATES: protein motif:HMM:NF021856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2437 domain-containing protein"
                     /protein_id="HRV71908.1"
                     /translation="MKIAHFSTLPDGGSLWGIIEKDMIHATEGPGGNRTGERFPISAA
                     RLRAPAFPGKIVCVGRNYYGHIREMGNDSEPLPEEPGLFLK"
BASE COUNT         2301 a         2337 c         2777 g         2086 t
ORIGIN      
        1 tttttaagat tttcctgcct cctgtgtcca gtctcctgct tcctcgcatt cacgattcac
       61 gaaaaccaga ttcccgccgg tgggcggacg ttccgcttcg ctacgctcag aatgacagca
      121 ggatcaaaag cgtaaacgtt gaaaggcctc ccgaagagcg gaatggcaga agagcaagga
      181 gataggagac cttaaaggcg taaacggtga acgggaagcc cgaggagaca ggagactgga
      241 ggctggaaac aggaagagca aggactttgt cattctgagg aatgaaatga tgaagaatcc
      301 gggttagttt ttaagatttt cctgcctcct gtgtccagtc tcctgcttcc tcgcattcac
      361 gactcacgat tcacgaatca cgaaaaagga tctgggtcgg tatttgaacc agaaccagat
      421 tcttcgtccc gttggccctc agaatgacag caggataaaa ggcgtaaaca gtaaacataa
      481 aaaggaggta acgcagatgc aggagaagtt cgagaacgta acgctggtta gcaaggccaa
      541 tgtttacatg gacgggaagg tgacaagcag gaccttcttc actccttcag gagagaggaa
      601 gaccgcgggg atcttccttc ccggcgaata cgaattcgga acaggggatg ccgaaatcat
      661 ggatgttgtc cagggaagtg ccacggtgct gctcccggga gaagccgatt ggaagacttt
      721 cggtcccggt gagagtttca acatcccgaa aaacagttcg ttcaaactca agatcgacgg
      781 cgttttcgat tatatctgtt cctatatcaa ggagtagtgc aaaggcgggg gccggggttc
      841 atccccggcc tacctgttgg agaaataaat gaaaaacatc actcccatga agaaacccgc
      901 ggaaatggcc ttgaccagtc ttgcgttgtc gatcctgtca tcttttgacg ggtcgtagta
      961 ctcctctcgc ctgatgtttt ttttcttttt ccccgtgatc ttgtcggcat aggaatacat
     1021 cagaaaaccg gcgcccagtc ccataaaaat ggccccgata gcggcctgca gcagagatct
     1081 ttccattcat gaatcccctt cccgtaaaaa aatcgggtat ccttgtacag atgctatcct
     1141 taaggggatc gacaggtcaa taccggtggt ttcgggtttc catcggggag ggagttacat
     1201 aatgaataaa tttgttcttc ttctcgctgt attttttgtt gtcttttccc ctgcgtcggc
     1261 tttctcctcg gaatgggaga gggtcggaga cgctgcctgg gccgaagtga gttcgatcga
     1321 agagttcgaa acggtccttg aggagatgct tggatccttc gaggagcacg ccgtgatccg
     1381 tctccccctg aagaggtgtc tggaggagga cgagatccag ggaatgctgc agagtatcta
     1441 ctaccgcagg gacctcgtag ctgcttcaat ggcgaggatg gaggccacaa taaggtatgc
     1501 ctccggcatg aacaccgtgg agtttctctt taaatggata gacagcaggc aacagcgcga
     1561 cgaggtgcgg gagatggttc cccgcatatt gtcgtcgatc cttgcccccg gaatgtcgga
     1621 gaggcagatc ctcgaggcga tccatgatca tgttgtttta tcggtcgttt acgatgattc
     1681 aagaatgggg cgcggcgcct gggaggcgat ccatggtaga gccgtctgcc aggggtatgc
     1741 cctcctcact gaggcccttc tttcggaggc gggcttcgag aaccgcgtag tattgaacag
     1801 cgaccacgca tggaaccttg ttttcacaca agggacatgg cggcatatcg atacggcttt
     1861 cgatgacccc atcataatcg ggggaggcaa gcctgaagga atatcccggg aggctttcct
     1921 gaaaacagat gccgaaatga gggcattggg tcataactgg gatgagaaac gatatccgaa
     1981 ggccaggtga taggccggga agcaagcccc tcccgttgag aagtttactc ctttgatctt
     2041 tcgatccccc tggctacgtc gaccttttga agcaagaaaa tgcccgccgc gaacagaacg
     2101 atcagagagg taatgcctag ccgtgtcgac cccgtcaatt gtgtcagtac tgcgaatatc
     2161 gttggtcctg ccactcctga gaacttgctg aaaatatcgt aaaatgcgaa aaactcccca
     2221 gtctgagcct tcgggaccat catcccgtag gtgcttctgc taatggcctg aacgcctccc
     2281 tgggccatac ctacggctat cgcaagaatc cagaagtgtg tcgccgtctc caggaaaaac
     2341 gcgccgatgg tcaccatcat gtaccagacc agtccgatgg ttatggttct tttcccgccg
     2401 agttttaccg atagccttcc gaagagtatg gcaaaggggg ctcctataaa ctgcgtcgcc
     2461 agcagtgccc cgacgaggtg catcattcct atgcctttct cggaaccaaa tatggcagcc
     2521 atcttcatta tggtcccgac gccgtcgtta taaagccagt atgcccccag gaatatgagc
     2581 atgtccctgt agttgcgcat cttcctgaac gtttgggcca gctggctgaa gccgcgggtc
     2641 ataacctcgt tccggttcag ggggcgaggc aaagccgacg ggggctcagg tattaacagc
     2701 atcagcggga tagtgaatgc gctccaccat attgccaccg acagaaacga tacccgtaca
     2761 ccgagggtcc ccggtatgat cgcggcggct gcgatgtttg ccgcaaggag agtccctccc
     2821 ccaagatagc cccatgcata ccctctcgat gagacaatgt ccatctggcc cggtggagct
     2881 atgtggggca ggagagcatc gtaaaaaatc atggatagcg aaaaccccag gcttccgcct
     2941 ccgtaaagaa ggagagccaa aaaccagtct ccctcttcca caaagaagag cagggctgta
     3001 gaaaacacac cgagaagggt gaaaaacgca aggaatttct ttttggcctc gccaacgtcg
     3061 gccatgctgc ccagtatcgg ggcgatcagg gctccggaca gaagcgctat ggatgatgta
     3121 tagccccaca aagccgatgg catgttcgga ggcaatcccg cggcggcgac ttccctgaag
     3181 tagacaggga gcatcgcggc cattatggta gtggcgaacg ccgaattacc cgcgtcgtaa
     3241 aggcaccagg cgttgatcat gcgacgattt ttattttcgg gtctcatggg gctcatttta
     3301 acccgaaccg ccatgggcga acgaaaagat tttttagcaa agaccggggg agcgtttttt
     3361 ggtatccttg tccgggtgct taatgtggct ttcgggaaga gccctcgaaa aaagatcaag
     3421 aggtggatct attgaagata agaactttgg atgggccccg ccttcacagg ggtttcaccg
     3481 ctggagcgag gaacattcag ataaggcagg aaacactgaa caggatgaat gtttttcccg
     3541 tccccgacgg tgacacggga tcaaatctgg ccgcgaccgt acagtctgtc tcggaaggcg
     3601 ctgtgataag ccgttcggca tcagatacca gcgcttcgat ggcggatgct gcccttctcg
     3661 gggcgagggg gaactccggg ctgatatttg cacagtttct ctatggtttt gctcaggagt
     3721 ccaacgggaa agacgagatc aacgcccgtg atttcgggag ggcggttgcc ggtgccatac
     3781 cctatgccag ggaggcgttg agcaaccccg ttgaggggac cattctgacg gtaatggaag
     3841 actgggcctc ggaggtcaaa agcattacaa gacagtccaa tgattttgcc cttcttttcc
     3901 ccgggtcgct cgaagtagct cgcagatctc tgcaggaaac accaaaaaaa cttgccgtgc
     3961 tggcaagggc cggtgtagtc gacgcagggg cacaggggtt cgtaaacttt ctggaaggga
     4021 tagtctcttt cctggagagc gggaacctga ggaactactc cgggcagatg gaactcccgg
     4081 tgatatccca cgtccatgat gatttctccg aaggtgagcc gtcccaccgt ttctgtaccg
     4141 aggcgctcct ctgcggagaa ggtcttgaca aggcacagat caggaaagag atgcagccct
     4201 tcggtgattc gatcattgtg gggggtcacg ggagcaaggt aagggtccat atccatacgg
     4261 atactccaga ccggctcttt ttctcaatga gaaagcatgg tgctctaaaa cagcaaaagg
     4321 ccgatgatat gaagaggcag gtggatgtct gcaggcgaag acttcattca gttgccattc
     4381 tcacggattc aacctgtgac ctccctgcgg aattcatgga cgagaaccag atccataccg
     4441 ttcccctccg tctggccttc ggtgacagtg tctttataga caaggtcacc atatcacctg
     4501 accagttcta tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcgcca
     4621 aggttttcgt cagcctgaaa accctcaagt atatggtccg gggaggcagg ctcagcccgg
     4681 taaagggttt tttggccgag gtgctgaatc tgaaacccat cataagcgtt gacgaagagg
     4741 gaaaagcctt ctccttcggc cagaccaggg gatgggatgc caacataagg aagatagaga
     4801 aggtaatctc ggagatgcag ggccagggac ggatatggaa ctactgtgtt gttcatgccc
     4861 attctcacga atccgccgaa cttgtggccg ccgggctcaa gaggatagtg ggcaaagccc
     4921 ctgcctacac catggatatt tgccccgttc tcgggacaca ctccggaata ggctcggttg
     4981 ccgttggcat cctgatggag tagacgggca atgtccaaaa agaagacggg tgaaaagaac
     5041 gcggaaatga aaaaggatac tctcgagagg ctggctgaag tgatggagcg atcgaaggtc
     5101 cttgagaata tcgagatgat cctcgatcgg cgtaaactgc tctggaacaa ctttctggct
     5161 ggactggcaa ggggcctggg cttcggggtc gggctgacgg ttcttgccgc tgtttttatc
     5221 tacattctgg tcgtgatcct gaaatcaata gtggctctca acatcccgct gatcggcaca
     5281 tacatcgctg atcttgtcga gatagtacag gaacaggtaa gcaatcatcg ctgatgatgt
     5341 cataataacg gaaagcgggg aattggtttt gtccagaaag atcgaacggg aagggaccat
     5401 ttcaacgata ggcaaaaaga gcgttatccc gaaagtgttc atggcagttg cggttctgtt
     5461 tgccctggct tctggagtgt acatagccgt ggatcacggg aaaacgtctg cggaaattac
     5521 agggaatgga tccgaaatcc cccccgcagc ggtggaggga ccttcgaagg ttccgcccct
     5581 ggcggatccc ggtatcgtcg cgccatttgt cgaagagcat ggactttccg gcaagttctg
     5641 gataatgatc gacaagagcg atttccttct caccacgtgg gaaggcgacc ggttcctttc
     5701 cggttatacc atagcaacag gtgagaatcc gggcgacaag gagcgtgttg gtgacagaag
     5761 aactcccgaa ggcgtcttca gtgtgaaaag tatccacgat tccagggcgt gggtccatga
     5821 tttcggtgac ggcaaaggcc cggtagaaca cgcctatggg ccatggttca tccggctcga
     5881 gacgggctgg aaggggatcg gtatccacgg gacacacgac ccgtccaccc tggggaagat
     5941 ggtcaccgaa ggttgcataa ggatgaacaa cgaggccctg ctcgagatag tagaaagggc
     6001 ggtgcctggg accgtcgttc tgatcgaacc ctaaaaagga aacggggtga gttttcaatg
     6061 aaggaccgga aggtacgggt tttttcgctg gttctcgcgg tacccctttt cgttgctgcg
     6121 ttctggatct tttcagcgga ggtaggagcg accgatgatc cggcggggaa acttttccag
     6181 gccctgaact gggtctcgga gtcgccggac agggcttggg ccagggtctc cctcggggat
     6241 gtctatgttt cactgggaag gcatgaggac gcgataaagg cctacaagac tgccctgcgt
     6301 tttgagccgg acaatgcctt tgccatgaac ggcatgggat acgcgtggct tggtctggga
     6361 aaacccgaaa aagctctgca attttttgtt gatgccgccg cagcggatcc ttcgaaggcg
     6421 gatctgaaga gcctggggta tacctggatg gctctcggca aaaacgccga agcgctggac
     6481 acgttcaatt cggtcctcgc gaaagagccg gagaacacgg agatactgaa tgccatgggg
     6541 caggccagtt actcgatggg agattttgcc ggggcggtga aatattttga aacggcgatc
     6601 agtgttgatc cccttaaggg agattatgcc agcctgggct actcatacag cgctcttggc
     6661 atggaggaca gggcaaagga atcttacagc gaaggggtgc gtcttcgccc cagggatcag
     6721 gaatcgctca acgccctcgg cttcagcctg ttctcccgcg gaaggctgga agaggccagg
     6781 gaaatttttt atgaatcact cgacatagat cccgataacg tctacgtcct gaacggcctg
     6841 ggctatgtct acagtgccgg aaaagaccac gaaaaggcaa ttgagtttct cgagaaggct
     6901 gccagggcag atcagtccca ggcggattac gttacgacag gctacgaata tgcggctctg
     6961 gaaagacatc gggaggcgga ggcggccttc aggcgagcca tttccaggga tccctcttcg
     7021 gccgccgaag cgtggtacgg ccttggctac acccttatcg cgatggggag caatgtcgag
     7081 gcaatggatg ccttcaggcg ttcctacatg ctgatgccgg agcccaggac cctcaacagc
     7141 cttggctatg cctacatggt ggtgagaaac taccccaatg ccatagaggc tttcaacagg
     7201 gctctgggga tagatccaga ggacgcgcag gccaggttca accttgccct ggccatgctg
     7261 gacctggaca ggagggccga ggccaggaaa tactacgggg aactgcagaa aatagacgag
     7321 gaactcgcgg cggaactgaa gagtatcatg gatgaggcct ggaaatagga tccaaaatct
     7381 gtaaggaaga gggtgagacc cgtgaaatac gagattatcg gagagggtgc ctatcctgcg
     7441 gtgaaactgg ctttggcaga aggagatgtt gtgaccgccg aggctggggc gatggcaagc
     7501 atggatggca gtgtcgagat acagacccag atgaagggtg gtctgctggg agggctcaag
     7561 aggtcgatgc ttggcggcga gagtttcttt atgaaccact tcacctcccg tgccgatgga
     7621 ggggaactgg tgcttgctcc cacactcccg ggtgctatca gggtaaggga gatgaagggt
     7681 gagacgattt tcctgcagtc gggctcatac cttggttcct tcggagatgt ggagatcgat
     7741 accaagtgga agggtttcaa gggggttttt gcgggagaag gttttttcat gcttcgtgtt
     7801 accggcagtg gaacgctttt cttcaacagt tttggagcta tgtacgacgt tcaggtaagc
     7861 gggtcaccat acaaggtaga caccggcaat attgtaatgt tcgaggaggg actcgattac
     7921 cgtgttgcgt ctgtaggggg tctcaagtcc ctggtgcttt cgggtgaggg actggtggcc
     7981 gagtttaacg gccacgggct tctcgtgatg cagacgcgga gcattccggg attcattgga
     8041 tggatcaccg gaaagatgcc caaaaagagc ggcaattgat aacgggtcca cggcaggatt
     8101 tttataacta aggagagtgg tcgctcttga gcctgcaacc atgggatgat ctttattctg
     8161 aggtaggcaa cgaactgact ccttccccga taagggaact tgtcaagtac atgaagaaac
     8221 cgggaatgat atcacttgcg ggcggcaacc cggatcccga aatatttccc gtggaggagt
     8281 ttcaggaagc cgcctcgatc attaccgaaa gaggcaagga gatccttcag tacggcgcca
     8341 cgggtggcta tgaacctctg aaggaattca tctccggatg gatggctccc cgaatgggaa
     8401 ggatcacggc ccttgacgaa atgtgtatca caacagggtc tcagcaggtt atggacctct
     8461 ttgccgcagt catgctggac ccgggtgatg ttgtgatcgt agaggagccg acataccctg
     8521 gtgccactca tgcgctgaaa aacagaaagg cccgttttat aaccgttccc tgcgacaaag
     8581 acgggatgca ggtggaactg atccccgaga tcctcgagca ggccagacag ggaagagaca
     8641 gggtcaaatt tatatattcg atagtaaatt tccagaaccc ttcaggatat acattatcag
     8701 ccccgagaag gaaaaaactt ctggaaatag cagtcgagta cggtattcca gtcttcgagg
     8761 atgatcccta cgggcatctc cggtacgatg gaccccatga accgaccatc ttctctatgg
     8821 acaacgatga tatggtcatc tacgcctgtt cgttctcgaa gattcttgct ccgggaaccc
     8881 gggtggcctg ggtaacagga ccgaaggaag tcatcaacaa gatggtgatg gtcaagcagg
     8941 gatcggatct atgcacgagc gtgctggccc agggactggt ctacgaatac tgcaggcttg
     9001 gccacctgga gtctttcctt cccaggatac aggcgcacta caaaaaaaag agagatgcga
     9061 tggaaagcgc cttcagggag tatcttccct ccagcgccat attctcaact cctgcgggcg
     9121 ggtttttcta ctggctcacc cttcccggga caaatacaag agatctcttc ttcaaggcga
     9181 tcgagaggaa cgtttctttc gttaccgggg atgctttcta tccgacaggt ggaggagaag
     9241 aggaactgag agcctgtttt tcctatgcca gggccgagga tttccccgag gcggccagac
     9301 gcctggccca ggccatcagc tcatgagagg tggtgttccc tccctgtgaa gatcgcccat
     9361 ttttccaccc tcccggatgg cggttccctg tgggggatta tcgagaagga tatgatccat
     9421 gccacagaag gcccgggtgg caacaggacg ggagaaagat tccctatctc tgcggccagg
     9481 cttcgggctc cggcttttcc gggaaagata gtctgcgttg gaaggaacta ttacggccat
     9541 atccgggaga tgggcaacga ttccgaaccc ctcccggagg agcccgggct ttttttgaaa
     9601 g
//
LOCUS       DAOTIQ010000063         9386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_8734_length_9386_cov_8.258922, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000063 DAOTIQ010000000
VERSION     DAOTIQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_9386_cov_8.258922"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="P5541_07710"
     CDS             <1..708
                     /locus_tag="P5541_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV71909.1"
                     /translation="SPHKGDWNSVVGDNTVSIFFAEHEAGFMAGMAMALQLKEGPVGF
                     VGGMEIPPVQKFNWGFQQGVEYANSNFGTKITMAQEDFLYQGSFDNVAAGQQIAASMY
                     DRGVRAIFAAAGGVGVGVINEAKSRAAAGKEVWVVGVDVDQFAEGIYADGKSIILTSA
                     MKRIDQAAYDMIKAELEGKFPGGQTLVFDAVNNGIGIPKENPNLSDEVQEKADEIFAA
                     MQKGEIAVSAEQGKLIK"
     gene            854..1666
                     /locus_tag="P5541_07715"
     CDS             854..1666
                     /locus_tag="P5541_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV71910.1"
                     /translation="MKLTVVVENFADTPNVIAEYGLSIHIQDDRTQVLMDTGQGEALI
                     PNMYALGIRPDKVDHLVLSHGHFDHTGGMQKFLLRSGNIPIWAHPDVEIAHTRARGGM
                     NRFIGCHLNKDAFDFRPVTGLTEISDGIWAVEVPVDHRDPAFLSSTGHLVLPSGKEWV
                     PDTFPDDISLVVKGDRGLSVVFGCAHAGTVNILEEVSLHFGTREFYSVIGGMHLGGSP
                     AEFVEMVTGELVSRFSVAWWKPCHCSGIATAISLSRRSANVSWAGAGTSHEI"
     gene            1840..3282
                     /locus_tag="P5541_07720"
     CDS             1840..3282
                     /locus_tag="P5541_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRV71911.1"
                     /translation="MPRIKMINLLLVIFLVFALFPAGGAECEEQPGIMFILDASGSMW
                     GQVEGRAKIDIAREVLRDLLEKMPQGLDLGLTAYGHRRTGDCKDVEKIVPIGPDSKNE
                     ILKHLAAIQPKGKTPIALSISQVAEDLKTREEETTVILISDGIETCEGDPCGVVKELK
                     AAGIKFVMHVVGFDTGKEEFDELNCIATEGGGQFFEAKDKDALLGSLESASSQAITKV
                     EPAVAATVTAVPSIGKLVIEIPEAGLKSLANIQILKGESPMKTIESLSPRSEHPLPPG
                     SYTLVFGYANPNYKDPSSVTVATFDVEKGSETIIAFGVLLYNLAEGLDEAVDSVIIHD
                     QVREADLVVNEYHGNGYYFFTPKPLPGGTYSVLLTHSRNPGRVTVARDMEIVSGKETV
                     LTLDSGFAVKKPGSSEMVGWDLLPAGGSTPFLSIRRKSDNDEPLWRRFMVPPGNYDLN
                     LIIKGMDQPLEIGQDISITPGSIVVFDTGM"
     gene            3409..4653
                     /locus_tag="P5541_07725"
     CDS             3409..4653
                     /locus_tag="P5541_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HRV71912.1"
                     /translation="MLLALCDCNNFFVSCERVRDPSLQGRPVIVLSHNDGCVVARSNE
                     AKALGIGMAVPFFSVKGLCVNRKVAVLGGDHRYYREMSGRVMALLMTMVPEVEVSSID
                     EAYLLLSGPHEEKVSAFCGSVRDSVWKSLGIPLSVGVSGTKTLAKAASHFAKKIPELN
                     GVCVLLPHTGEDMLSGMDARDVWGVGSRGAETLRRSGVKTALKLREFDEDRAERIFPL
                     GIRRAIAELNGRTCYPFASEGARRKTVQVAPSFSGQCGSLEELQDAAASYAAEAAATM
                     RSEGQVCRTVQVGLATDPFSRNQAYLEDTGEVSLEEPTDYTPDIARAARKGVAGIFRP
                     TTMYKRVSVILKDLERSDMMQMSLFRDEKCERNLRLMKALDGINSRLGSGTVKLGSQY
                     TDPKERGITPAEKPGRKVLDPE"
     gene            complement(4711..6054)
                     /locus_tag="P5541_07730"
     CDS             complement(4711..6054)
                     /locus_tag="P5541_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV71913.1"
                     /translation="MIKTDVLVLGGLSGITAGVSCRRHYPDKKIMLVRKEGTVLVPCG
                     IPYIYGTVGGPQNNVVPDGLLENNGIELVKGEAIGVDLEKKVVTLKNGETVGYEKLVF
                     ATGSVPIVPPIKGVDKQNVFPVKKEFNYLQEVLDRMPSVRNLVIIGGGFIGVEFADEC
                     RKGRDISVTVVEALPHCLQMALDDEFCSEAQEKLREAGVNLLCNSKVEAILGDDSVSG
                     IRLSDGREIPADMVILGIGASPNTDLAKKTGLELGFRSAIKVDRYMRSYTDPNVFACG
                     DCAEKLSFFTGRPVGTMLASIATSEARIAGANLFSPYLQNKGTISVFSTMINGRAFAA
                     AGLTERFTRENGMNIVVGEAQAPDTHPGGMPDSSLTKVKLLFEKETGIIVGGSISGGR
                     SVGEMINVLSACILHKMTADDITSFQMGTHPALTSSPIMYPIVSAACKASLPIRQ"
     gene            6343..7845
                     /locus_tag="P5541_07735"
     CDS             6343..7845
                     /locus_tag="P5541_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV71914.1"
                     /translation="MILFRMDERSGVSKSSQIVEQIRNMISSGVLRAGERIPSSRKMA
                     DILQVHRSTVSSAYQDLWALGYLEMRSGAVPLVRERFPVVSRKGSSNESTIDWASFSS
                     PSTEELWSDYSLARGRRPAGENLPGGTISFVPFRMDKRLFMLKSFRTCLTRSFREEGA
                     NLLSYGDVMGYGPLRETIANRMAAHGMDSHSDEILVTNGAQHALDLVLRMMSAPGRSV
                     AIEVPGYSQMIPLLRHHGLEIVEIPMKDDGMDLDLLEETCRVKPPHLVYTMPNFQNPT
                     GITTDQPHRERLLSLCSHYGVTILEDGFEEEMKYSGKVVMPIKSMDSENRVIYCGTFS
                     KVLFPGLRIGWIGAHRDCISRLSALRRFGELSPPSLLQAALQRFCADGHYERHVSSMH
                     RIYRRRMQSALAAMQRYLPAGRVGWTKPRGGYLVWLTMSTPPRTILWDDFFVQAGVDL
                     CHGEEFFPGSPSRKHFRMSISCLDEDEIEEGVRRLGLALRQIYGHETAAS"
     gene            7856..8335
                     /locus_tag="P5541_07740"
     CDS             7856..8335
                     /locus_tag="P5541_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV71915.1"
                     /translation="MSRGKIRITEEKVDPVTFNELRRSVGWETHREEDLVLATENSTF
                     FVTARDATTGDVVGMGRIIGDSGMFFYIQDIIVRPRWQGMGIGGMIMDRIMEHIRENG
                     REGLFVGLMSAEGKEGFYARYGFKKRPAPGLGAGMCLETENVNKKIIHNRGRSEREW"
     gene            8329..9249
                     /locus_tag="P5541_07745"
     CDS             8329..9249
                     /locus_tag="P5541_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRV71916.1"
                     /translation="MVRIGIKQVDAFTKDPFGGNPAGVVTVADSLDEEQMQSIAREMN
                     LSETAFVAPSKRADFKVRFFTPYSEVDLCGHATIGSFHALHEEGRLDPGRSVFSQETK
                     AGVLEVERIFVDGRPVFMMTQAKPLFEKTDASREEIAGILRVDAGELADTLPLKVSTG
                     IWWFVAGLKRLDTVMNLKPDFAAMSSFSERHGLVGMIPFSLQALDPSFTFHMRAFAPL
                     VGVNEDPVCGTGNGCAGAYIGQNRLVEFEKSADLSGEAGYEVQRPGQVLISLLREGES
                     ISQVKVGGSAVTILEGEMVFQGPCRSSNRC"
BASE COUNT         2169 a         2324 c         2740 g         2153 t
ORIGIN      
        1 tcgccccaca agggtgactg gaactccgtg gtcggtgaca acacggtctc catattcttt
       61 gccgagcatg aggccgggtt catggcaggc atggccatgg ctctgcagct caaggaaggc
      121 cccgtgggtt tcgttggcgg aatggagata cccccggtcc agaagttcaa ctgggggttc
      181 cagcagggag tggaatatgc aaacagcaac ttcgggacaa agataaccat ggcccaggag
      241 gattttcttt accagggatc cttcgacaac gtcgcggccg gccagcagat agccgcttcg
      301 atgtatgacc gcggagtcag ggccatcttt gccgctgccg gtggagtggg cgtgggtgtc
      361 atcaacgaag ccaagtcccg tgccgcggcg ggcaaggagg tctgggtagt cggtgttgat
      421 gttgaccagt ttgccgaggg gatatatgcc gacggcaagt cgataatact tacatctgcc
      481 atgaagagga tcgaccaggc cgcttacgac atgataaagg cggaactgga gggcaaattc
      541 cccggcgggc agactcttgt gtttgatgcc gtcaacaatg gaatagggat cccgaaagag
      601 aacccgaacc tctctgacga ggtccaggaa aaggccgacg agatatttgc tgccatgcag
      661 aagggcgaga tcgccgtatc cgcggagcag ggaaaactca tcaagtaggt atccttcctc
      721 tgtccgtgcc ccctgtgggg gcacggaccc ttttacaggt ttttgcttct cctgccaatg
      781 aacttttttc tgctgtcact gtggctgccg gatcttatgg ggataaaata gtgtcgccgg
      841 atgggggaaa tcgatgaagc taacagtagt agtggagaat tttgcggata caccgaatgt
      901 gattgcggaa tacggtctga gcatacatat acaggatgac aggacgcagg tactcatgga
      961 caccggccag ggggaagcct tgatccccaa catgtatgcc ctgggaataa ggccggacaa
     1021 agtggaccac cttgttctca gccatgggca tttcgaccac accggaggca tgcagaaatt
     1081 tctgcttcgg tcaggaaaca tccccatctg ggcccatccc gatgtggaga tagctcacac
     1141 cagagccagg ggaggaatga accgtttcat aggatgccac ctcaacaaag atgccttcga
     1201 tttccgcccc gtcaccggac taaccgaaat atccgatgga atatgggcag tggaggttcc
     1261 tgtggatcac cgtgatcctg ctttcctcag ttcgacaggt catcttgttc tcccctcggg
     1321 aaaagaatgg gttcctgata ccttccccga tgacatatct ctcgttgtca agggggacag
     1381 gggcctgagc gttgttttcg ggtgtgccca tgcgggaaca gtaaatattc tcgaagaggt
     1441 ttctttacac ttcggcacca gggagtttta ttcggtaatt ggaggtatgc atctgggagg
     1501 ctctcctgcg gaattcgtgg aaatggtgac aggagaactg gtttcccggt tcagtgtggc
     1561 atggtggaaa ccttgtcatt gctcgggtat cgccacggcc atatcgcttt ccagaagatc
     1621 ggcaaacgta tcctgggccg gagccggaac ttctcacgag atctagttcg accaggtcaa
     1681 tctgaccgga tttctcccag agaactttcg ggcctcgttg caaacactga acggggcttt
     1741 ttttatctct tttgcagagt agatgtggta ttgtatcagt gaggtgtgga gcaagatctg
     1801 cgatgggcga tgaaaccatg ctgaaggacg gtgggaaaaa tgccgcgaat aaaaatgatt
     1861 aacctcttgc tggtgatctt tctggttttt gccctgttcc ccgcgggagg ggcggagtgt
     1921 gaagagcaac ccggaataat gtttatcctc gatgcttcgg gaagcatgtg gggtcaggtc
     1981 gagggcaggg ccaagatcga tattgccagg gaggttctca gagacctgct tgaaaagatg
     2041 ccccaggggc tggatcttgg gcttacggcc tatgggcaca ggcggacagg cgactgcaag
     2101 gatgtcgaaa agatcgttcc cattggaccg gacagtaaaa acgaaatact gaaacacctg
     2161 gcagccatac agccaaaggg caagacaccg atagcgctgt caatcagcca ggtggccgag
     2221 gatctcaaga cccgggaaga ggagaccact gttatactga tcagcgacgg catcgagacc
     2281 tgtgaagggg acccctgtgg ggtcgtgaag gaactaaagg ctgccggtat caagtttgta
     2341 atgcacgtgg tgggtttcga cacaggcaaa gaggagttcg atgaactcaa ctgcattgcc
     2401 accgagggcg gggggcagtt cttcgaagcc aaagacaagg atgctcttct cggttccctt
     2461 gagtcagcca gcagccaggc tatcacgaaa gtggagccag ccgtagctgc gacagttacg
     2521 gcggtgcctt caatcgggaa actggttatc gaaattcctg aagcaggctt gaaaagcctg
     2581 gccaacatcc agatactcaa gggcgaaagc ccaatgaaaa cgatcgagtc cctttcaccc
     2641 cgttcggaac atcccttgcc tccaggatcc tacaccctgg tgttcgggta cgcgaatccc
     2701 aactacaagg acccatcttc ggtaacggtg gcgacctttg atgtggaaaa gggatctgag
     2761 acgatcattg ccttcggggt tctcttgtat aaccttgcgg aagggctcga cgaagccgtc
     2821 gactccgtaa taatccacga tcaggttcgc gaagcggatc ttgtagtcaa cgagtaccat
     2881 ggcaacggtt actatttttt cactccaaaa ccacttccag ggggcactta ttcagtcctg
     2941 ctgacccact cacgcaatcc gggcagagtg accgtagcca gagacatgga gatagtctcc
     3001 gggaaagaga ctgttcttac gctggattcc ggttttgccg taaagaaacc agggtcatcg
     3061 gagatggtcg ggtgggatct gctgcctgcc ggtggttcca ccccgttcct ttccatcagg
     3121 cgtaagagcg acaatgatga accgctgtgg agacgtttca tggtcccgcc gggcaattac
     3181 gatctgaacc tcatcataaa ggggatggac cagccccttg agatcggcca ggatatatcc
     3241 ataacacccg gatccatcgt tgtatttgac acgggtatgt aaggggttcc caatttcgaa
     3301 tgagtgatga accattgcag gtaataaaaa aagagaaact ggaaaaaccg ggaaaaaatg
     3361 ccccgacgcc catggcggcg caagttttcg tgggaaaggt ttggcataat gctgcttgcc
     3421 ctgtgcgact gtaacaattt tttcgtttcc tgcgagaggg tgcgcgatcc ctcactacag
     3481 ggaaggcccg tgatagtcct ttcccacaat gacggctgtg tggtcgccag gtcgaacgaa
     3541 gccaaggctt tgggcatagg gatggctgtg cccttctttt cggtaaaggg gttgtgtgta
     3601 aacagaaaag tggccgttct gggtggggat caccgctact acagggagat gtcaggcagg
     3661 gtgatggctc tgcttatgac gatggtgccc gaagtggaag tatcgtcaat agacgaggcc
     3721 taccttttgt tgagcggccc tcacgaagag aaggtatccg ccttttgcgg ctccgtaaga
     3781 gattccgtct ggaagagtct gggaataccc ctctcggtcg gcgtatccgg cacaaagact
     3841 ctggcgaagg ctgccagcca cttcgcgaag aaaattccgg aactgaacgg ggtttgcgtt
     3901 ctgcttcccc ataccggaga agatatgcta tccggtatgg atgccaggga tgtatggggt
     3961 gtcggctcgc gtggggcgga aacccttcgc aggtcaggtg tcaaaaccgc actgaaactg
     4021 agggagttcg atgaagacag ggcggagaga attttcccgc ttgggataag gcgcgcgatt
     4081 gcggaactca atggaaggac ctgttaccca tttgccagcg aaggggccag gcgcaagacc
     4141 gtgcaggtcg cgccctcttt cagcgggcaa tgtggctctc tggaggaact ccaggacgcg
     4201 gctgcatcct acgctgccga agccgctgct accatgcggt cggaagggca agtctgccgc
     4261 acggtgcagg tgggccttgc taccgatcca ttttcccgga accaggcata cctcgaggat
     4321 acaggggaag tatccctgga ggagcccacg gattatacgc ctgatattgc ccgtgccgcc
     4381 agaaagggag tggccggtat cttcaggccg acaaccatgt acaaaagggt ctcggtgata
     4441 ctaaaggacc tggaacgatc cgacatgatg cagatgagtc tttttcgcga tgaaaaatgt
     4501 gagcggaacc ttcgcctgat gaaggctttg gacgggatca acagcaggct gggaagcgga
     4561 actgtaaaac tgggttcaca gtacactgac ccgaaagaac gtggcatcac cccggcggaa
     4621 aagccaggcc gcaaggtgct ggatccggaa tgaatcagct tttttgccga taacactttt
     4681 cccctgccgt tgggtaggct tcttggggaa ctactgtctg atgggcaatg atgccttgca
     4741 agcggcgctg acgatggggt acataatcgg ggaagatgtg agcgccggat gggtccccat
     4801 ctggaaactt gtgatatcat ccgccgtcat cttgtgcagg atacaggcgc tcaggacgtt
     4861 tatcatctct ccgacggatc ttccgccaga gatggaaccg ccaacgataa tgccagtctc
     4921 cttctcgaag agaagtttga ctttcgtcag gctggagtcg ggcatcccgc ccggatgggt
     4981 gtctggcgcc tgcgcctctc cgacgactat attcatcccg ttttcccttg tgaaacgttc
     5041 agtcagcccg gcagcggcga acgcacggcc gttgatcatg gtcgagaaga cgctgatagt
     5101 ccccttgttc tggaggtaag gggagaaaag attggctccg gctatgcgcg cctcggaggt
     5161 tgcgatggaa gcgagcattg tgcccacagg tcgccccgtg aaaaatgaca acttctcagc
     5221 gcagtccccg caggcaaaaa cattggggtc ggtgtaactt ctcatatagc ggtcaacctt
     5281 gatggcgctc ctgaagccca gttcaaggcc cgtttttttt gcgaggtcgg tattgggaga
     5341 tgcccctatg ccgagaatga ccatatctgc cgggatttcc cgaccgtcgg acagcctgat
     5401 accggaaacc gagtcatctc cgaggatggc ctcaaccttt gagttgcaga ggaggttcac
     5461 tcccgcttcc ctgagttttt cctgggcttc ggagcaaaac tcgtcgtcca gggccatctg
     5521 caggcaatgc gggagagctt cgacaacggt gacgctgatg tcgcgccctt ttcgacattc
     5581 gtccgcgaat tccacgccga taaaaccacc gccgatgatc acgaggttcc tgacggatgg
     5641 cattcggtcg agaacttcct gaagataatt gaactccttc ttcaccggga aaacattctg
     5701 tttgtcaaca cccttgatcg ggggtacgat cggcaccgaa ccggtcgcaa ataccagttt
     5761 ttcgtaaccc accgtttccc cgttcttgag cgtaacgacc ttcttttcaa gatccacccc
     5821 gatggcctcg cccttgacga gttcaatccc gttgttttcc agtaatccgt cggggactac
     5881 attgttctgg ggtcctccca cggtcccgta gatgtatggg attccgcagg gaacaagtac
     5941 cgtaccttcc ttgcggacaa gcatgatctt cttgtcagga tagtgtcttc tgcaactgac
     6001 tcctgccgtg atgccagaaa gacccccaag gaccagaaca tcggtcttga tcataaagat
     6061 ctcctccctt gattttcctg tcattttttc ggactatcgt cctgaataag gaaccgggaa
     6121 gcattataga gcaaaatacc ccaaggggta aatatctcac gaagtaaaac cacccgatgg
     6181 taatgatccc cgttcaccat ccaaaccaat tgaactttcg tgaggtctta cccgggggca
     6241 acttgcggaa gtggatggat aaaacaactc tgaactggct gtagcggaca ggccgcttag
     6301 gaggtattgt ttccctggat gtcatttcag ggggggagaa gaatgatact tttccgtatg
     6361 gacgagagat cgggtgtctc caaaagcagt cagatcgtcg aacagatacg gaacatgata
     6421 tcctccggcg ttctccgggc gggtgagagg ataccttctt cgaggaagat ggccgatatc
     6481 ctgcaggtcc acagatccac tgtttcttcg gcgtaccagg atctctgggc attgggatat
     6541 ctggagatga gatccggggc tgtccccctg gttcgggagc gtttccctgt ggtttcgagg
     6601 aaggggagtt cgaatgaaag caccatcgac tgggcttctt tttcttcccc gtctacggaa
     6661 gaactctgga gcgactattc cctggctcgg ggcaggaggc cggccggcga gaatcttccg
     6721 ggaggaacca tttcctttgt tcccttcagg atggacaaaa gactttttat gctcaagtcc
     6781 ttccgtacat gtctgacaag atcttttcgc gaggagggag cgaatcttct ctcatatggc
     6841 gatgtgatgg gatatggtcc gttaagggaa acaatagcga accggatggc tgctcatggt
     6901 atggattcac actccgacga aatactcgtt actaacgggg cacaacacgc gcttgatctt
     6961 gttctccgaa tgatgtccgc gccgggcagg tcggtggcca tagaagtgcc ggggtactcc
     7021 cagatgatcc ccctgttgcg tcaccatggt ctggaaatcg tggagatacc gatgaaggac
     7081 gacggtatgg atcttgatct cctcgaggag acctgcaggg taaagccgcc tcaccttgtt
     7141 tataccatgc ccaatttcca gaatcccaca gggataacta cggaccagcc acacagggaa
     7201 cgtcttctct ctctgtgttc gcactacggg gtgacgatcc tcgaggacgg gttcgaggag
     7261 gaaatgaaat attcgggcaa ggtagtcatg cccatcaaat caatggacag cgagaacagg
     7321 gtgatttact gcggaacctt ctccaaggtt cttttccccg gtttgaggat tggctggata
     7381 ggggctcaca gggactgcat cagccgcctt tccgcccttc gccgcttcgg ggaactttca
     7441 cccccatccc tgcttcaggc tgctctccaa agattctgtg ccgacggtca ctacgagcgg
     7501 catgtttcct cgatgcacag gatctaccgg cggagaatgc agagtgccct ggctgcgatg
     7561 cagagatatc ttccagccgg acgggttggc tggacaaaac cacgtggagg ttatcttgtc
     7621 tggcttacca tgtctacgcc gcccaggacg atcttgtggg atgacttttt tgtccaggca
     7681 ggagtcgatc tctgtcacgg agaggagttt ttcccgggtt ccccgtcgag aaaacatttc
     7741 cgcatgtcca tttcctgcct tgacgaagat gagatcgagg agggagtcag gaggcttggt
     7801 ctggctctcc ggcagatcta tggccacgaa acggcagcca gttgaggttt tgaggatgag
     7861 caggggcaag atccggatca ctgaagaaaa ggttgacccc gtgaccttca acgaactgag
     7921 gagatccgtg gggtgggaga cccacaggga agaagacctg gtattggcca cggagaacag
     7981 tacctttttt gttacagcca gagatgccac cacgggagat gtggtcggta tggggagaat
     8041 aatcggtgat tcgggcatgt tcttttacat acaggacata attgtcaggc ccaggtggca
     8101 gggtatgggc atcggaggaa tgataatgga caggatcatg gagcatatca gggaaaacgg
     8161 cagggaaggt ctctttgtcg gattgatgtc cgcggaaggt aaagagggtt tctacgccag
     8221 gtatggtttc aaaaagcgtc cggctccagg cctgggggcc ggtatgtgtc tggaaacaga
     8281 aaacgtgaac aaaaaaataa tccacaatcg ggggaggtct gaaagggaat ggtgaggatc
     8341 gggataaagc aggttgatgc atttacgaaa gatccttttg gtggcaatcc ggccggcgta
     8401 gtcactgtgg cggattctct ggacgaagag cagatgcagt ctatcgcgag ggagatgaac
     8461 ctgagcgaga cggcgtttgt tgctccatcg aaaagagctg atttcaaggt tcgcttcttt
     8521 accccctatt ctgaggtcga cctttgtgga cacgcgacca tagggagctt tcatgccttg
     8581 cacgaggagg gacgcctcga tcccgggcgt tctgtttttt ctcaggagac gaaggccggg
     8641 gtgctcgaag tggagcggat ttttgttgac ggtcgccccg tttttatgat gacccaggca
     8701 aaaccgcttt tcgaaaaaac agatgcgtca agagaagaga tcgccggaat attgagggtt
     8761 gatgcaggcg aactggccga cacccttccc ctgaaagttt cgacagggat atggtggttc
     8821 gttgcaggac tcaaacggct ggatactgtc atgaacctga aaccggattt tgccgcaatg
     8881 agcagtttca gtgaacgtca cggacttgtc ggaatgattc cgttctcttt gcaggccctt
     8941 gacccttcgt tcacttttca tatgagggct ttcgctcctc tggttggagt gaacgaagat
     9001 cctgtctgcg gtacgggtaa cggttgtgct ggtgcctata tcgggcaaaa caggctggtg
     9061 gaatttgaaa aaagtgcaga cctctccggc gaggccggtt acgaggtcca gcgccccgga
     9121 caggttctga tatccttgct ccgcgagggc gaaagcattt cgcaggtaaa ggtcggcggt
     9181 tcggcagtta ccatcctgga aggggagatg gtttttcagg gtccatgccg cagttcaaat
     9241 cgttgttgat caggtccggc cgtggtttcc ggttttggag gcagtgaagt ttttctgatc
     9301 ctgccagtat tgccggggcc cttgcgggtc ccggcaattt gaagattatt ccttccgaag
     9361 gtagtttgct gcagtctgtg acaaca
//
LOCUS       DAOTIQ010000064         9112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_9103_length_9112_cov_8.113062, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000064 DAOTIQ010000000
VERSION     DAOTIQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9103_length_9112_cov_8.113062"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..78
                     /locus_tag="P5541_07750"
     tRNA            3..78
                     /locus_tag="P5541_07750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Arg,seq:cct)
     gene            137..790
                     /locus_tag="P5541_07755"
     CDS             137..790
                     /locus_tag="P5541_07755"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRV71917.1"
                     /translation="MNDWTGKARNMVRNQILYRGMAEGPITLAMEKVPRHLFVPLSVR
                     EFAYEDRPLPVGFGQTISQPWIVASMTALLEISPGMKVLEIGTGSGYQAAILSFLGAR
                     VVSVERIMPLMKEAKKTLSSIAPDVRVYFGDGRKGHLAEAPFDRILITAASDAIPSPL
                     LEQAKEKAIIVAPVRRENGAERLMVKRIDGILQSVTWGEYCRFVPLLENTSKTGDYS"
     gene            819..1328
                     /locus_tag="P5541_07760"
     CDS             819..1328
                     /locus_tag="P5541_07760"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HRV71918.1"
                     /translation="MLKKKAVFFLISMVSAVLMAATYPVDVLGITEGDSVYYRRPVPV
                     GFRFVSRYIHSVEKTPVEDDYRVVGGSIWSWEERVLSQNAGMPFVRPRNGRLIMDEDW
                     MRFRGGRYSWEDLYVRIGDNRFGENEMFGHSPWTGYFRLFELLPSRRLLFSVRKDSLF
                     LALLKGVEL"
     gene            1416..3398
                     /locus_tag="P5541_07765"
     CDS             1416..3398
                     /locus_tag="P5541_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRV71919.1"
                     /translation="MAEKHNDDVNNSETSQAGSQSIDLDELRRQFDTEARTRMLGGWQ
                     AMMVLVAAVSLSLFHFYTSGFGLLLAIKQRSVHLAFVLFLVYMLYPAGAKARKDKVAW
                     YDFILAGLAVYAVLYHVIFFNDIIMRSGLPTTMDLAMGFIGITLLLEATRRVSNPILP
                     GIAIFFLFYCYFGRHFPAMFAHRGFNITRIINHMYMGTEGVFGIPLGVSSTFVFMFIL
                     FGSFLEQTGMGRFIIDLSMALAGGSTGGPAKVAVLSSGLMGSISGSSVANVCTTGMFT
                     IPLMKSVGYKPYFAGAVEAVASTGGQIMPPVMGAAAFIMAEFLGVPYIQVAMAAIVPA
                     LLYYFAVIVQVHLEATRLGLKGLPREKLPKVWPLLKTKGHLLIPLVAIVYFLLAGFTP
                     LKAAYNGILATLIVSFFNKETRMTIPKLKDALDSGARGALGVACACATVGIIVGTATL
                     TGLGLRIASAIITLSGGVLIISLMLTMVACILLGAGLPTTANFIVTSTMAAPALLQLG
                     VAPMAAYMFVLYFGIAADLSPPVALAAYAGAGIAGDDPMKTGGTAIRLAIAGFLVPYI
                     YVYNPMLVLVGFEVIPFTISIGTALLGVFLLGAGSIGFYKVSMPLWLRAVAVAGALNL
                     LVPGIYSDAIGFAVLAGIHIIQVRKARSLDNPAVTG"
     gene            3497..6295
                     /gene="ileS"
                     /locus_tag="P5541_07770"
     CDS             3497..6295
                     /gene="ileS"
                     /locus_tag="P5541_07770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRV71920.1"
                     /translation="MTTDYKNSLNLPVTSFPMRANLAKREPEFLSFWEKENIYDKLVE
                     KGTGKPEFILHDGPPYANGNIHIGTAFNKILKDLIPKYKWMRGFRSPYLPGWDTHGLP
                     IELHVLREKKATKDSIDPVELREHCQKYAEKFLDIQRKEFKRLGVLGDWDNPYITFKP
                     EYEAAQVGVFADMMEKGLVYKGRKPVFWCIDCQTALAAAEIEYEDETSHSIFVAYPME
                     GCGNSFFKDAAGGRECFVLVWTTTPWTLPASLAVAVHPELEYVFVPSGNKIYIMAKAM
                     VARVSAETGINFEEPVFSVKGKDLESARAVHPFYEDRIIPFALADYVGTDQGSGCVHT
                     APGHGVEDFETGIRYGLEILNPVDDRGYFLPETKIVGGLSLEDATEKILTLLKESGRL
                     LGSGKIRHSYPHCWRCKKPVIFRATEQWFVSVNDFRKKAMEEIDNVSWVPGWGKERIS
                     NMVRDRSDWCISRQRIWGVPIPAFYCESCGKVVADPERIRKVQALFREKGSNSWWRLD
                     PPEILGDLSLCPHCGGSKLRKETDIMDVWFDSGCSHVAVLETRPEMVWPADLYLEGSD
                     QHRGWFQTSLLTSVATREKAPFKTVLTHGFIVDGEGRKMSKSIGNVIAPQEIIEKFGA
                     DILRLWVASTDYRNDVRISDQILANLVESYRRIRNTARFMLGNLAGFDPSLMTDFKDM
                     PEMDQWILSKVQRLISRATKGYDEFEFHVPTYMIHHFCVNDLSAVYLDVCKDRLYAES
                     KDGEARRSCQSVMWIMLGALTRMLAPVLSFTSEEIWQEMRKIDPSLEESVFLAPWPVI
                     DESIPAIDIESRWETILDVRGAICRALESARSTGIIGHSLDAEVFSLSKGGDKDPSSM
                     LAEKDWETLVIVSSFRFGEKPEGEGYIWEDQETGLEFFVRKAPGNKCPRCWKISSGVS
                     GDSVCTRCREVLERSATQ"
     gene            6333..7283
                     /locus_tag="P5541_07775"
     CDS             6333..7283
                     /locus_tag="P5541_07775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRV71921.1"
                     /translation="MPEFIVTEETYRLTVGGENEGERLDLFLAWKLDKTRSRIQSLVK
                     EGKVSLFPPRNPKSSMLVKKGMEITVRVEPRQTADLRPEPVTFGVVYEDEDLLVVDKP
                     AGLIVHPSPGHWTGTLVHGLLHRFPEIGDMGATERPGIVHRLDGSTSGLMVVSRNEKA
                     HELLSRAFQERRVMKDYLALVWGKPKKREGTIDLPVGRDPRNRYRMAVTPGGKKAVTL
                     YRTIWSRAGFSLVTCRLMTGRTHQIRVHMKHIGCPLVGDRLYAPRKKDFAGFDRVFLH
                     SWILGFEHPKRGLTMDFRSFLHPELKEFLINVLSREQARP"
     gene            complement(7234..8889)
                     /locus_tag="P5541_07780"
     CDS             complement(7234..8889)
                     /locus_tag="P5541_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HRV71922.1"
                     /translation="MPLAPELFNEIRTSLAERFSGRICSSFDSGSSWFSLTFRPSGEN
                     LFFSWSDSFQGICTASREQVSFLSASDRSNPLIRDVLNKYFQNFRLEKVSRSKGDRIL
                     ILDFRKMVGAGIGVGSTIVAEFSGRAPNVIFLRDDGTVIESARSVSSGGYDSLRYRPG
                     EPYLQPDPFIGRELSDSCDPSIFFSLDSLSGYGKKAGEAINNSWHHYPLSSWKSFFMT
                     SQYVREPGERSSAIIQSLNGVLSVFPIQTINGERAGEDLLRTLGKFVVEPLFHDFFKH
                     KKRRYISQIKKSISRLRSLKKGYRNRMEQSLKAEEYKLAGNLLLTWKKRVPKGASSVS
                     LPLWNDNGGDTVIPVDPSKTVSANAQDYFRKFRKYSVDPEKIEKELDEVISRENDLAA
                     LMSRIQNAPDPGELSVLARELEEATVRTKNRKSHGPVKVYDLPGNQILAGSSAKGNRK
                     VTFVLADGDDLWFHARGVPGGHVILKKTIAGGFDDVIVIQIAASVAAWLTRSREAEKV
                     AVDYTFRKYVRPIPGTTAEVTYSRSRTITVSPVLWKEHLSETP"
BASE COUNT         2247 a         2144 c         2355 g         2366 t
ORIGIN      
        1 gcgggcctgt agctcagagg atagagcaac ggcctcctaa gccgtaggtc aggcgttcga
       61 atcgcctcag gcccgccaat ttaaggtctt ttgccgggga acacccctca ccctccattc
      121 gggaggtggt aaccagttga atgactggac cggaaaagcc aggaatatgg ttcgcaacca
      181 gattttgtat cgaggaatgg ctgaaggtcc gataactctt gcgatggaga aagtccccag
      241 acatcttttt gttccccttt cggtcagaga attcgcgtat gaggatcgcc cacttcccgt
      301 cggattcggt cagaccattt cccaaccctg gatcgtggct tcaatgacgg ctcttcttga
      361 gatctctcct ggaatgaaag tccttgaaat agggacggga tcgggttacc aggcagcgat
      421 tctttccttt ttaggggcca gagtggtttc cgtggaaaga ataatgcccc tcatgaagga
      481 ggccaaaaaa acactttcct cgatcgcccc tgacgtgcgc gtgtatttcg gggacggaag
      541 gaaaggacat ctggctgaag ctccctttga caggatcctc attacggctg cttccgacgc
      601 aatcccatct ccccttcttg aacaagcgaa agaaaaggcg attatcgttg ctcctgtcag
      661 gagggaaaat ggcgcggaga ggttgatggt caaaagaata gatggcatac ttcaatccgt
      721 cacatgggga gaatactgcc gttttgttcc acttcttgaa aacacctcca aaacagggga
      781 ttatagttaa aaaccattgg ttggggagaa tctatgttgt gttaaagaaa aaagcagtgt
      841 tttttttgat ctctatggta tctgccgtcc taatggcagc gacctacccg gttgatgttc
      901 tgggaataac ggagggagat tcggtatact ataggagacc ggtaccggta ggattccgtt
      961 tcgtttcccg ttatatccat tcggttgaga aaacacccgt agaggacgat taccgggtcg
     1021 tcggcggaag tatatggtcc tgggaagaaa gggttctttc acagaacgcc ggaatgccat
     1081 ttgtccgccc aaggaacgga agattgataa tggatgaaga ctggatgagg ttccggggag
     1141 ggaggtattc ctgggaggat ctctatgtca ggattggtga caaccgattt ggcgagaatg
     1201 agatgtttgg gcattctcca tggaccggat attttcgtct tttcgaatta cttccatcaa
     1261 gacgccttct gttctccgtc aggaaggata gtttgtttct tgccctgctg aagggagtcg
     1321 aactgtgata attaatgcct ctgtgtggta aaatcaatct gttttatttt tggaaggact
     1381 tttatttctg gtaattattt tatgggggga taagcgtggc agaaaaacac aacgatgacg
     1441 ttaataattc tgaaacgtcg caggccggtt cccagtcgat agatctggat gaactgagaa
     1501 gacagtttga cactgaagcc aggacaagaa tgctgggcgg atggcaggcc atgatggtcc
     1561 tggtcgctgc ggtctcactc tctcttttcc acttttatac ttccggtttc ggccttcttc
     1621 tcgctatcaa gcagaggtcg gtccatcttg catttgtcct gttccttgtt tacatgcttt
     1681 acccggccgg tgcaaaagcc agaaaggaca aagtggcatg gtatgatttc atccttgccg
     1741 gcctggcggt ctatgctgtt ctatatcacg taattttctt caatgacata atcatgagat
     1801 cgggtcttcc caccactatg gaccttgcca tgggatttat agggattacc cttctgcttg
     1861 aagctactcg aagggtttcc aatcccatcc tgcctggaat agcgatcttt ttcctttttt
     1921 attgctattt cggaaggcat ttcccggcaa tgttcgccca cagaggtttt aatattactc
     1981 gtattatcaa tcacatgtat atggggaccg agggtgtctt cggtattccg ctgggtgttt
     2041 cctcaacctt tgtcttcatg ttcatcctct ttggatcatt ccttgaacag acgggtatgg
     2101 gacgattcat tatcgatctt tcgatggccc ttgccggtgg aagcaccgga ggccctgcca
     2161 aggtggcagt tctcagctcc gggctgatgg gttccatttc cggctcttcc gttgccaacg
     2221 tttgtactac tggaatgttc acgatcccgt tgatgaagag cgttggctac aaaccctatt
     2281 ttgcgggagc ggttgaagca gttgcctcaa cgggcggaca gataatgcct cccgtcatgg
     2341 gagccgccgc atttatcatg gcagagtttc ttggcgttcc ctatatccag gtcgcgatgg
     2401 ccgctatcgt ccctgcactg ctttattatt ttgccgttat tgtgcaggtc catctcgagg
     2461 caacgagact gggtcttaag ggactgccga gggagaaact cccaaaggta tggccccttc
     2521 tcaagaccaa gggacacctc ctgatccctc tcgtcgcgat agtctatttt ctccttgccg
     2581 gttttacacc gttaaaggcg gcctataacg ggatactggc cacactgatc gtatcattct
     2641 tcaacaagga aaccaggatg acaattccca aactcaagga tgccctcgac tctggtgcaa
     2701 gaggcgccct gggggttgcc tgtgcctgtg ctactgtagg tatcattgta ggtactgcga
     2761 cccttaccgg actgggattg aggatcgcca gcgcgatcat tacgctgtca ggaggggtcc
     2821 tgatcatatc ccttatgctc acaatggttg cctgcatact tcttggcgcg ggacttccca
     2881 caacagccaa ttttattgtt accagcacaa tggcggctcc agcccttttg cagcttggag
     2941 tagccccgat ggcggcatac atgtttgttc tgtacttcgg catcgccgct gatcttagcc
     3001 cccctgtagc ccttgcagcc tacgctggag caggaatagc cggggacgat cccatgaaga
     3061 ccggaggaac agccataagg cttgcgatag caggttttct tgttccttac atctatgttt
     3121 ataaccccat gctggtcctt gtagggtttg aagtgatacc cttcactatt tccataggga
     3181 ccgctcttct cggggtgttc ctgcttggtg caggatcgat aggcttttac aaggtttcca
     3241 tgcctctctg gctaagggcc gttgccgttg ccggagcact caaccttctg gtaccgggta
     3301 tctattccga tgccataggt tttgcggtcc ttgcggggat acatataatt caggtcagaa
     3361 aggcacgcag cctggataac ccggcggtta ctggctgacc aggagtaaac tgtgcatgct
     3421 tctgatccat gaatgaacct gaggaggccg tcccggtccc gggacggcct ccttctatcc
     3481 aaggaggcga aatttcatga caacggatta taaaaattct ctgaatctcc cggtaacatc
     3541 atttcctatg agagcgaacc ttgccaaaag ggaacctgaa tttttatcct tctgggagaa
     3601 agaaaatatc tacgacaaac ttgtggagaa ggggacggga aaacccgaat ttattcttca
     3661 tgatggcccc ccctacgcga acggcaatat tcacatagga acagcattca acaagatcct
     3721 caaagacctg ataccgaaat acaagtggat gagaggcttc agatctccct atctgccggg
     3781 atgggatacc cacggattgc cgatcgagct ccacgtcctt cgtgaaaaga aggcaacaaa
     3841 agattcgata gaccctgttg aactcaggga gcactgccag aaatacgcag aaaagttcct
     3901 ggatattcaa cgcaaggaat tcaaaagact gggtgttctg ggtgactggg acaacccgta
     3961 tattactttc aaaccggagt atgaggctgc ccaggtagga gtatttgccg acatgatgga
     4021 aaaaggtctt gtctacaagg gccgaaagcc ggttttctgg tgcattgatt gtcagacagc
     4081 cctagctgca gcggaaattg agtatgagga cgaaacctcc cattccatct ttgttgccta
     4141 ccccatggag ggctgtggca actcattttt caaggatgcc gccggaggca gggagtgttt
     4201 tgttctcgta tggaccacca caccctggac gcttcccgca agccttgccg tggctgttca
     4261 cccggaactg gaatatgttt ttgttccctc cggcaacaag atctatatca tggccaaggc
     4321 aatggtagcc cgcgtctccg ctgaaacagg aataaatttt gaagaaccgg ttttttccgt
     4381 caagggaaag gatctggaat ccgcaagggc agttcatccc ttttacgagg acaggatcat
     4441 cccctttgcc ctggccgatt atgtcggtac agaccagggt tccggatgtg tccatacagc
     4501 cccgggacac ggtgtggaag actttgaaac cgggatcagg tacggtcttg aaatccttaa
     4561 ccctgtagac gaccggggat atttccttcc cgaaacaaaa atagtcgggg gcttgtccct
     4621 tgaagatgcc acggaaaaga tcctgaccct ccttaaagaa agcggaaggc tgttgggaag
     4681 cggaaagatc cggcactcct atccacattg ctggagatgc aaaaaaccgg tgattttccg
     4741 ggccacggaa cagtggttcg tctctgtgaa cgatttccga aaaaaagcca tggaagagat
     4801 agacaacgtt tcatgggttc ctggttgggg aaaggaaagg atttccaaca tggttcggga
     4861 ccggtccgac tggtgcataa gtcgccagag gatctgggga gtccccatcc ccgcctttta
     4921 ctgcgagtct tgcgggaagg tcgttgctga ccccgagagg atcaggaaag tgcaggctct
     4981 tttcagggaa aagggctcaa actcctggtg gagattggat ccaccggaaa tattgggaga
     5041 cctttccctt tgtcctcatt gcggggggag taaactgagg aaggaaacgg acatcatgga
     5101 tgtatggttc gattcagggt gcagtcacgt ggctgtcctg gagacgagac cagaaatggt
     5161 ctggccagcc gacctctatc ttgagggaag cgaccagcac aggggatggt tccagacctc
     5221 acttcttact tccgtcgcga caagggagaa agcccctttc aagaccgttc tcacacatgg
     5281 attcattgtg gatggtgaag ggcgtaaaat gtcgaaatcg atcgggaatg tcatagcccc
     5341 ccaggagata atagaaaaat tcggggcgga catactgcga ctctgggtcg catcgacaga
     5401 ctacaggaat gatgtccgta tatccgacca gatccttgcc aatcttgtgg aatcctacag
     5461 gaggatcagg aacaccgcaa ggttcatgct tggtaatctg gcgggttttg acccatctct
     5521 catgacagat ttcaaagata tgccggaaat ggaccaatgg atactttcaa aggttcaaag
     5581 actcatatca agggctacca agggttacga cgagtttgaa tttcatgttc ccacctacat
     5641 gatccatcat ttttgtgtga atgacctgag cgccgtatat ctggatgtct gcaaggatcg
     5701 cctttatgcc gagtcgaaag atggagaagc gcgaaggagc tgccagtcgg ttatgtggat
     5761 aatgctgggc gcactaacta gaatgctggc tccagttctt agttttacat ccgaggagat
     5821 atggcaggaa atgagaaaga tcgacccctc ccttgaggaa agcgtttttc ttgcaccctg
     5881 gcctgttatt gacgagagca ttcctgccat agatatcgaa agcaggtggg aaacaatcct
     5941 tgatgttcgc ggtgctattt gccgtgctct tgaatcggcc aggtcgacag ggataattgg
     6001 ccattcactg gatgcggaag tgttctccct ttcaaaagga ggagacaagg atccttcttc
     6061 aatgcttgcg gaaaaggatt gggagactct cgttattgtc tcttctttcc gttttggaga
     6121 aaaacctgag ggtgagggtt acatctggga agatcaggaa acgggtcttg agtttttcgt
     6181 caggaaggct cccggaaaca aatgccccag gtgctggaag atttcttccg gggtttccgg
     6241 ggattcggtc tgtacaagat gccgggaagt acttgaaaga tctgctacac agtaaactgt
     6301 tatgcagaaa cggggaaaaa tggtgtgaaa cattgcctga gtttattgta acggaagaaa
     6361 cttacaggct gacagtgggg ggggagaacg aaggagagag gcttgacctt ttccttgcat
     6421 ggaagctgga caaaacaaga tccaggattc agtccctcgt caaggaagga aaggtgagcc
     6481 tctttcctcc aaggaacccc aaatcctcca tgctggtaaa aaaggggatg gagatcaccg
     6541 ttcgagtgga accgaggcag acagctgatc tccgtcccga gcccgtgact tttggcgtag
     6601 tttacgagga cgaagatctt ctcgtggtag ataaacccgc agggcttatc gttcacccgt
     6661 caccgggaca ctggaccggg acgcttgttc atggtcttct tcacagattt cccgaaatag
     6721 gagatatggg ggctacggaa agacccggga tagttcacag gcttgatgga tctacttctg
     6781 gccttatggt tgtatccagg aacgaaaagg cgcacgaact tctttccagg gccttccagg
     6841 aaaggagggt gatgaaggat taccttgctc tggtgtgggg caaaccgaaa aaacgggaag
     6901 gcacaataga tcttcccgtc ggtcgtgatc caaggaacag atacaggatg gctgttacac
     6961 ccggcgggaa aaaggcggta actctttacc gcacaatttg gagccgggcg ggtttttcac
     7021 ttgtcacttg cagactaatg acaggcagaa cacaccagat aagggtccat atgaagcata
     7081 tcgggtgccc ccttgtcggc gacaggctct atgctcccag aaaaaaggat tttgcgggat
     7141 tcgacagggt tttcctgcat tcctggatcc tgggttttga acacccaaaa aggggactta
     7201 cgatggattt ccggtccttt ctgcatccgg aactcaagga gtttctgata aatgttcttt
     7261 ccagagaaca ggcgagaccg tgatggtcct ggaccgactg tatgtaactt ctgccgtggt
     7321 tcccggaatc ggtctcacgt atttcctgaa ggtataatcc actgctactt tttctgcctc
     7381 ccttgatctt gtaagccatg ctgctacgga cgccgctatt tgaataacaa taacgtcatc
     7441 aaaaccaccg gcaattgttt ttttcagaat tacatggcct ccggggaccc cccttgcgtg
     7501 aaaccacaga tcgtcgccgt cagcaagaac aaaggtaact tttctgttgc ccttggcact
     7561 tgaaccagcc agtatctggt ttcctggcag gtcgtagacc tttaccggac catgggattt
     7621 cctgtttttg gttcttaccg ttgcctcttc caattccctt gcaagaactg aaagctcccc
     7681 agggtcgggg gcgttttgaa ttcgtgacat taatgcggca agatcatttt ctcttgaaat
     7741 gacctcatca agttcttttt caattttttc gggatcaacg gagtatttcc tgaatttacg
     7801 aaaataatcc tgggcattgg cggaaacagt ttttgacggg tccaccggta tcactgtgtc
     7861 ccctccattg tcattccaca ggggaagaga aacagagctg gcaccctttg ggactctttt
     7921 tttccaggtc aagaggagat ttccggcaag tttgtattct tcagctttca aagattgttc
     7981 catccggttt cggtaacctt ttttaagtga tctcagtctt gagatggatt tttttatttg
     8041 agaaatatat cttctttttt tatgtttaaa aaaatcatgg aaaaggggtt caacaacgaa
     8101 tttgcctaga gttcggagaa gatcttcacc ggcccgttct ccattaatgg tctggatcgg
     8161 aaaaacagaa agaacgccat tcagagactg gataattgcc gaagatctct cccccggctc
     8221 tcttacgtat tgtgaagtca taaaaaaact tttccaggag gataaaggat aatgatgcca
     8281 ggaattgttt atggcttcgc cggctttttt cccatatcct gacagactgt caagagagaa
     8341 aaaaatggat gggtcgcaag aatctgatag ttccctgcca ataaagggat ccggctgaag
     8401 atagggttcc cctggacgat aacgcaggga atcatagcct ccggaagata ctgatcgtgc
     8461 ggattcaatt acagttccgt catccctgag aaagatcacg ttgggagctc ttcctgagaa
     8521 ttcggcaacg atcgtcgaac ccaccccgat ccctgcacca accattttcc tgaaatccag
     8581 aataagaatc cggtctcctt tactccggga aactttttca agacggaagt tttgaaaata
     8641 cttgttcaga acatccctga tcaggggatt gctcctgtcc gaggctgaaa gaaaagaaac
     8701 ctgttcccgg cttgctgtac agataccctg aaatgagtca ctccaggaaa aaaacaggtt
     8761 ttctccggag ggtctgaagg tcagcgaaaa ccacgatgac ccggaatcga aagaggagca
     8821 gatcctgcct gaaaaccttt cggccaatga tgttcggatt tcgttaaaca gttccggtgc
     8881 tagaggcatc tttgttctcc tgtaaatgaa ttggtgagaa aatactttcc cggttcacag
     8941 tacatatttt aacctctgtg cagaaaatcc gttcatggca atatatttat catgttagag
     9001 ctgatttgcc ttgacaacac tccgttccat ctctagaatt atcgccgtca cttggaaaag
     9061 gggctgtggc gcagcgggag cgcgcttcct tcgcacggaa gaggccgggg gt
//
LOCUS       DAOTIQ010000065         8763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_9601_length_8763_cov_8.001263, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000065 DAOTIQ010000000
VERSION     DAOTIQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9601_length_8763_cov_8.001263"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1879)
                     /gene="typA"
                     /locus_tag="P5541_07785"
     CDS             complement(5..1879)
                     /gene="typA"
                     /locus_tag="P5541_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRV71923.1"
                     /translation="MYEVEKIRNLAIIAHIDHGKTTLIDSIFRAARVFRDNARVDERV
                     MDNNELERERGITIRSKHCTVKWKDYLINLVDTPGHADFSGEVERVLSTVDSVLLLVD
                     ANEGPMPQTRYVLRHALKMGLRPLVFINKADRPGANIDDALNKTFDLFIELGATDEQT
                     DFPVIYGSGLQGWAVRDLADQAPEGNMDALFDMIIEHVPAPRAILDAPFLMQVTTLAW
                     NDYSGRIGCGKVLQGSLLKGTDFLKTSSRWLDPKKQEGEWEVTGCSREKAAQLWVTRG
                     LERVAVEEVTAGDIVWISGPKEIGIGDVFSSPEIQDPGMAPLSIEEPTISMFILVNNG
                     PFSGQEGQAITLRQIKARIERETKSNIALHMEDLGRPDGVKISGRGELHLAILIEEMR
                     REGSEFCVSRPEVITEIIDGKIFEPNETLTIDVPEEFQGIVFEKISRRRGRMTGMQHL
                     PGGTMLRMEFSIPTRGLIGYRGDFLTDTRGLGIMSSCFDGYAPWAGEINPRNRGSVVS
                     LETGEATAYQIENLQERCTLFIRPMERVYEGMIVGENSRPGDMICNPTKKKQMTNHRA
                     STQDQYTKLDVPRTMTLEKAIEWIAQDELVEVTPSSIRIRKAILDENERKKRLHKAVS
                     "
     gene            complement(2024..2428)
                     /locus_tag="P5541_07790"
     CDS             complement(2024..2428)
                     /locus_tag="P5541_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71924.1"
                     /translation="MRKLVWILVIVGLAAGYLVYTGDIRFSKPPEQKTTENLEKERDA
                     IVADLSIRLEQIRKGMEDLKKQASLKTGEGRETLDKKIADLQAEWDEARSRMDRIASS
                     GADQWQSIVESTVSAFDRMKKAYEAAREELSK"
     gene            2573..3259
                     /locus_tag="P5541_07795"
     CDS             2573..3259
                     /locus_tag="P5541_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV71925.1"
                     /translation="MTNNSSGRRYLSLVLFLFMTVSMVAVFAIGPLWAEGERYDRMEV
                     PSPAEMAKEEEYCVSSARIQVIVSAFTRVNRGLDHSTAASYASHVLEASENFGIHPFM
                     IASIIIRESTVKPNARSRYAYGLMQINWKAHRNGLKKAFSQIQTLDDLLKPRNNILAG
                     TWIFSWYLKSSDGDIKKALAKYLGRTGSKYINRVLAGYNAMLQEFEKNQDLVRTQIAD
                     ISPSIAYAVK"
     gene            3303..4376
                     /locus_tag="P5541_07800"
     CDS             3303..4376
                     /locus_tag="P5541_07800"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRV71926.1"
                     /translation="MPASLPILSIGKHTPRFPLIQGGMGVRISGPGLAAAVAKAGGIG
                     TIASVGLAVASESFDGRNYFEVNQVVLREEIQKARAASDGGIIAVNAMVALTDYDLHI
                     KAACEGGVDMIISGAGLPLRLPELTKDFPDVALVPIVSSTKAANLIVRRWDKLYGRLP
                     DAFVVETPLYAGGHLGVTKMEQVMDEALSLESVVPELVSYIRDEVKADIPVIGAGGVW
                     NREDMNKVFALGAKGVQMGTRFATTLEGDADIRFKQALIDASEEDVVIIKSPVGIPGR
                     ALRSPFIEKYLEGTVESKPCFANCLSHCSYRTDKQAFCIAQALVDAYRGNWEEGLFFC
                     GSNVGKIEKMENVEEIFRDLFGD"
     gene            complement(4733..5989)
                     /locus_tag="P5541_07805"
     CDS             complement(4733..5989)
                     /locus_tag="P5541_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV71927.1"
                     /translation="MQYFWTRFTSSEEHPDRGLRNGLRALRSRNYRLFFVGQSVSLIG
                     LWIQRVALGWLVYRITLSPLSLGFVDFIGQFPIFCLTLFTGIWLDRMDLRRLLIVTQW
                     LAVAQALVLAALTLSGAITYRQIVFLSAFLGIVNAFDMPARQAFVIHLVNRKEDLGNA
                     IALNSSLFNGARLIGPSIAGIAIAAVGEGVCFLVNGISFLGTIVALRAIRTKPLERVE
                     KKRTGMSAVLEGIRYAAGNRPIRALLLLLTLISLAGLPYLVILPVFARDVLGGGPMTL
                     GYLMACSGIGALGGTFFLASRRSAIGLRKVIAYATMIFGVALSLFSISGTPWLSFPLI
                     AIAGFGMVLTVSSCNTLLQTLVDDRMRGRVMSLYVMSLIGTGPLGSLMAGTAARFAGA
                     PVTFFVGGLVCAAAGWKFAGKIRRNR"
     gene            complement(6020..7855)
                     /gene="pepF"
                     /locus_tag="P5541_07810"
     CDS             complement(6020..7855)
                     /gene="pepF"
                     /locus_tag="P5541_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRV71928.1"
                     /translation="MNNFSMTLEGTTTAGDIPEREDLDPRFMWRLSDLFEDDKAWENS
                     FAETMALIPSLGSFSGNISASHANLLECLRLSDRIGESVSRIYVYAHMKSHEDTRKQD
                     PQSMASRAESLAVMASEAGSFIVPEILSMTDSEISALVAREKGLEMYSFFLDEISRKR
                     KHILSQKEESLLAASGEVTRAPETIFSMLTNADMTFPPIVGENGEETPFSEERYAVFI
                     QSKNRRVRQSAYSTLYGTFGKYRNTLSASLNAAIRSELFQSKARGFSSNLEASLYEDD
                     IPTDVYSSLIDGVRRHIDLLHRYVDLRKRVLDLDELRMFDLYVPIAPESRQDIPYSDS
                     VSMVREGLRPLGPRYLAALDEGFSEGWVDVYENKGKRGGAYSWGSYPTHPFVLMNYSG
                     RLRDVFTLAHEMGHALHKYFSDLNQPYIYAGHSIFIAEVASTTNEALLLEHLLKTASG
                     REERLYLLNYRLEQIRTTVFRQTMFAEFERIVHGMVEKGDAPNAGTFCSVWRELNSDY
                     YGPGISLDEEIEMEWARIPHFYSPFYVFQYATGYSAATALSRLILQEGDKATKRYIRF
                     LERGRSAPAIDVLRDAGVDMTLPDPVDKTLDLFRTTLDEFEKLVF"
     gene            8109..8543
                     /locus_tag="P5541_07815"
     CDS             8109..8543
                     /locus_tag="P5541_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71929.1"
                     /translation="MKRSGSLALFAVVFCFLAGIVSLAEAATVTWWYDVQVTGTRYLF
                     NPATGNVDQTPLSSSGLAKIEFNDAMRGGPVVIQTPVLGYTEGKRTGWNFSGERAYAG
                     MLFKTSGVFAPGNISGTIVVDDLSKSGSKGHYEYQFHGVRKN"
     gene            8577..>8763
                     /locus_tag="P5541_07820"
     CDS             8577..>8763
                     /locus_tag="P5541_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71930.1"
                     /translation="MRKTAIATSVVLLILAFAGAVSAADISGNWDVKVHGYNYFTDRT
                     PSRVPIAETETTMKIEQD"
BASE COUNT         2046 a         2238 c         2332 g         2147 t
ORIGIN      
        1 ggttctagga gacggctttg tggagtcgtt tcttcctttc gttttcatca agaatagcct
       61 ttcggatcct tatcgatgac ggggtgacct cgaccagttc gtcctgggct atccattcga
      121 tggccttttc gagggtcatt gttctcggta cgtccagctt cgtgtactgg tcctgtgtag
      181 aggcccgatg gttggtcatc tgttttttct tggtcgggtt gcagatcata tctcccgggc
      241 gcgaattttc ccccacgatc attccctcgt aaaccctttc catcggtctg atgaatagcg
      301 tgcaccgttc ctggaggttc tcgatctggt aggcggtcgc ttctcctgtt tcaagactga
      361 cgacggaacc cctgttcctt gggttgatct ctccggccca tggagcgtag ccgtcaaaac
      421 aggaggacat tatcccgagt ccccttgtgt ctgtaagaaa atcgcccctg tagccgataa
      481 ggccgcgggt gggtatggaa aactccattc ggagcattgt gcccccgggg agatgctgca
      541 tgccggtcat gcgtcccctc cgcctggata ttttttcaaa tacgatcccc tggaattcct
      601 cggggacatc gatggttaat gtttcatttg gttcgaagat ttttccgtcg attatctcgg
      661 ttattacttc tggtcttgat acacagaatt cggagccctc tcgcctcatt tcctcgatca
      721 gtatcgccag gtgaagttcc cccctgccgg agatcttgac cccgtccggt cttcccagat
      781 cttccatgtg gagggctatg ttgctcttgg tctctctttc gatcctggcc tttatctgcc
      841 tgagggtgat ggcctgccct tcctgacccg aaaagggtcc attgttgacc aggatgaaca
      901 tggatatggt cggttcctcg atggaaaggg gagccatccc tggatcctga atctccggtg
      961 aagaaaatac atcacctata ccgatctcct tggggccaga gatccagaca atatcaccag
     1021 cggtgacctc ttcgacggca actctctcga ggcctctggt aacccacaac tgcgcggctt
     1081 tttcccggga gcagccagtt acttcccact ccccctcctg ttttttcggg tcaagccagc
     1141 gggaagatgt ttttaaaaaa tcggttcctt tcagaagtga tccctgaagt accttgccgc
     1201 agcctattct tccggaataa tcgttccacg cgagggtggt gacctgcatc aggaacgggg
     1261 cgtccaggat agcgcgcggg gcgggaacat gttcgatgat catatcgaag agggcatcca
     1321 tatttccctc cggagcctgg tcggcaaggt ctcttacggc ccaaccctgg agcccggagc
     1381 cataaataac cggaaaatcc gtctgctcgt ctgtggcgcc cagttcaata aaaagatcaa
     1441 aggttttgtt gagggcatcg tcgatattcg cgcctggacg gtcggctttg tttatgaaca
     1501 cgagaggtct gagccccatc ttcagagcgt ggcgaagaac atacctcgtc tgcggcatgg
     1561 gtccctcgtt ggcgtcaacc agaagaagca cggaatcgac ggtggagaga acacgctcga
     1621 cctctcccga aaaatcggca tgtccaggcg tatccacgag gttaatgagg tagtctttcc
     1681 atttaaccgt gcagtgcttg gagcgtatgg ttatgccccg ctccctttca agttcgttgt
     1741 tatccatgac gcgctcgtct actcgggcgt tgtcacgaaa aacccttgcg gccctgaaga
     1801 tcgagtcgat cagggtcgtc ttgccatgat caatatgggc gataatcgcc aggttgcgga
     1861 tcttttcgac ttcatacatt cttttacatc tccccggagc gaatagtgcg aatacacctg
     1921 aaagcagggt cacacagaaa cggaatgata caacctgcgg cgcataaata caatagattg
     1981 ctacggattc tggtgcgatc ctggtccgaa aaaacgggtt tatctacttc gagagttcct
     2041 ccctggccgc ctcgtaggct ttcttcatcc tgtcaaatgc cgaaacggtg gattccacta
     2101 tcgactgcca ttgatccgct ccggaggaag cgatcctgtc catacgggat ctggcttcat
     2161 cccattcggc ctgcagatcc gctatttttt tgtccagggt ctccctgcct tccccggtct
     2221 tgagagaggc ctgttttttg aggtcttcca tacctttccg tatctgttcc agccttatcg
     2281 aaagatccgc gacaattgcg tcccgttcct tctcgaggtt ttccgtggtt ttctgttccg
     2341 gaggtttgga aaaccttata tctccagtgt agacgaggta accggcagca agccctacga
     2401 tgacaagtat ccagacaagt ttcctcatgt tattgttgcc ccttccttgt ttcattattt
     2461 tttgccggga ttaaacacga cattagtccc ttgcccaaag gtgtttccaa tactacactt
     2521 tctcttgagt gtggcacaac aggatcagag tattttcggg gggtaatttc aaatgacaaa
     2581 caactcatcg ggcaggagat acctgtccct ggttttgttc ctgttcatga ccgtttctat
     2641 ggtcgccgtt tttgccattg gtcctctttg ggccgagggg gaacgatacg acaggatgga
     2701 ggtgccttct cctgcagaaa tggcgaagga ggaggagtac tgtgtctcat cggccaggat
     2761 ccaggtaatt gtttcggcct tcacccgcgt taacagggga ctcgatcact caactgccgc
     2821 ctcctatgcc tctcacgttc ttgaggcttc agaaaacttc ggcatccacc ctttcatgat
     2881 cgcatccatc ataatcaggg aatcaacggt caagcccaac gcccggtcca ggtatgccta
     2941 tgggttgatg cagataaact ggaaggctca caggaacggc ctgaaaaagg cattcagcca
     3001 gatccagact ctggatgacc ttctcaagcc acgcaacaac atccttgccg gaacctggat
     3061 tttttcctgg tatctcaaat ccagtgatgg ggacataaag aaagctctcg caaaatatct
     3121 tggaagaacg ggcagtaaat atatcaacag ggtgctggcc ggttacaatg ccatgcttca
     3181 ggagtttgag aaaaatcagg acctggtcag gacgcagatc gctgatattt caccttccat
     3241 tgcttatgct gtaaaatagg ctggtttttc gttttgaaat tgaatccaaa ggagcggtta
     3301 tcttgcctgc aagtctgccc attctttcta tcggaaagca cacacccagg ttcccgctta
     3361 tccagggtgg tatgggagta cgtatctccg gtccgggcct cgctgctgct gtagccaagg
     3421 caggagggat cggaaccatc gcaagcgtgg gactggcagt tgcctccgaa agtttcgatg
     3481 ggaggaacta tttcgaggtt aaccaggttg tgctcaggga agaaatacaa aaagccagag
     3541 ccgcatctga tggcggcata attgctgtca acgcaatggt tgcccttacg gattatgatc
     3601 tccacatcaa ggccgcctgc gaaggtggag tggatatgat catttccggt gcggggcttc
     3661 ctctccgtct ccctgaactg acaaaagatt tccccgacgt agccctggtt cccatagtca
     3721 gttccaccaa ggcagccaac ctgattgtca gacgctggga caaactctat ggccgccttc
     3781 ccgatgcatt tgtggttgag acccctcttt acgccggagg tcatctcggg gtaacaaaaa
     3841 tggagcaggt catggacgaa gcgctctcgc tggaaagcgt cgtgccggaa cttgtctcct
     3901 acatccggga tgaggtaaag gcggatattc cggtgatcgg tgccggtggg gtatggaatc
     3961 gtgaagacat gaacaaggtc ttcgctcttg gggcgaaagg ggttcagatg gggaccaggt
     4021 ttgcgaccac cttagaaggt gatgccgata taaggttcaa gcaggccctc atagacgcca
     4081 gcgaggaaga tgttgtcatt atcaagagcc cggtcggaat accgggaaga gctctgagat
     4141 cgcctttcat tgaaaaatat cttgaaggca cagtggaaag caagccatgc ttcgccaact
     4201 gcctttctca ctgttcctat cgaactgaca agcaggcctt ctgcatagcc caggctcttg
     4261 tggacgcgta caggggaaac tgggaagagg gtctgttctt ctgcggttcc aacgtcggca
     4321 agatagaaaa aatggagaat gtggaagaga tattccggga tctcttcggg gattgagaac
     4381 aggaaacaac aggggcgtga atcgtaaaag tacgcaaatc gtgaaaacaa ggcgtcagta
     4441 gactgaggtt tcctgttatt gctgagttga ccttgttgac ctcgttgact tgtcgacctt
     4501 attatcgttg gggtcgatcc tgtcgaccgg gtcaatgagg ttttgtcatt ctgaggacca
     4561 acgggaggaa gaatccggtt taggttccgg ttcttccaaa gaaaggtttt ctctgtgaaa
     4621 ctctgcgata tctctgcggt ggaaaggtct ggctcttgcc ctggcatttg gtcctgacaa
     4681 aaacaatatc ctctctgttc aactctgtgg agagaagttt ttcttcctgt tcctacctgt
     4741 ttcgccggat cttccctgcg aacttccagc ccgctgcggc gcagacaagc cctcccacaa
     4801 agaatgtaac cggagccccg gcaaacctgg ccgcggtccc ggccatgagg ctccccaggg
     4861 gaccggttcc gataagggac atcacgtaga gactcatcac ccttccacgc atacggtcgt
     4921 caacaagggt ctgcagcaag gtattgcagg aggagacggt caggaccatc ccgaaacccg
     4981 ctattgctat aagagggaac gaaagccagg gagttccgga gatcgagaaa agggagagcg
     5041 cgaccccgaa tatcatcgtc gcgtaggcta ttacctttct cagtccgatg gcgctcctgc
     5101 gggatgccag aaaaaaggtt cctcccagtg cgccgatgcc ggagcaggcc atgagatatc
     5161 ccagggtcat aggtcctccg cccagaacat ctcttgcgaa aaccggcagg ataaccaggt
     5221 agggcaggcc ggccaggctg atgagagtca acaggaggag cagtgcccgg atcgggcgat
     5281 tccccgcggc gtacctgatt ccctccagta ctgctgacat gccggttctt ttcttttcga
     5341 ccctctcgag aggtttcgtc cttatcgcac gaagggctac gatggtcccg aggaaagata
     5401 tcccgttcac gagaaaacag acaccctccc cgacggccgc gatggctata cccgctattg
     5461 atgggcctat cagcctggcc ccgttgaata gagacgagtt tagcgctatc gcgtttccca
     5521 ggtcctcctt gcggttgaca agatggatga cgaaggcctg ccttgcgggc atatcgaaag
     5581 cgttaactat tcccaggaag gcgctcagga aaactatctg cctgtatgtt attgctcccg
     5641 aaagggtcaa cgctgccagc acaagagcct gcgctaccgc aagccactgg gtaacgataa
     5701 gcagcctgcg cagatccatt ctgtctagcc atatcccggt aaaaagggta aggcagaata
     5761 tggggaactg cccgataaaa tcgacgaagc ctaaagaaag gggcgaaagg gtgatcctgt
     5821 agacaagcca tcccaaagcc acacgctgga tccacagccc tatcagtgaa acactttgac
     5881 ccacgaaaaa aaggcggtag tttcgcgagc gaagagcgcg aagtccattt cgcaggcctc
     5941 tgtccggatg ttcttcggaa cttgtgaatc ttgtccagaa atattgcaag gttctgcccc
     6001 tttcttttgg ccccgggtat caaaagacaa gtttttcaaa ctcatccagc gtggtccgga
     6061 aaaggtcaag ggttttatca acagggtccg gcagagtcat atctacgccg gcatcacgca
     6121 ggacatctat tgcaggagcc gacctgcccc tctcaagaaa acgtatataa cgttttgttg
     6181 ccttatcccc ttcctgcagg atcagcctcg aaagggccgt ggcagcggag tatcctgtcg
     6241 cgtactggaa aacatagaag ggagagtaaa agtgaggtat ccttgcccac tccatctcga
     6301 tttcttcatc cagagagatg ccgggtccgt aatagtcgga gttcagttct ctccagacag
     6361 aacagaaagt cccggcgttt ggcgcgtcac ctttttcgac catcccgtga acgatcctct
     6421 cgaactccgc gaacatggtc tgtctgaata cggtcgtccg gatctgttcc aggcgatagt
     6481 tgaggaggta gagacgttct tccctgccgg aggcggtctt gagaagatgc tcaagaagaa
     6541 gggcttcgtt cgtggttgaa gccacttcgg caataaatat cgaatgcccg gcataaatat
     6601 aaggctggtt cagatcggaa aagtacttgt gaagagcatg tcccatttcg tgggcaagag
     6661 tgaaaacgtc cctgagcctg ccactgtaat tcataagcac gaaggggtgc gtcgggtagg
     6721 atccccaact gtaggctccc ccgcgtttgc ccttgttctc gtaaacgtct acccatccct
     6781 cactgaatcc ctcgtcgagc gcggccaggt atcgaggccc caaagggcgg aggccttccc
     6841 tgaccatgga taccgagtca ctgtaaggga tgtcctgtct tgattccggt gctatcggga
     6901 cgtacaggtc gaacatcctt agttcgtcca gatccaaaac ccttttcctg agatcaacat
     6961 aacggtgcag aaggtcgata tgcctccgta ccccgtcgat cagggaagag taaacatctg
     7021 tggggatatc gtcttcataa agggaagcct caaggtttga ggaaaatccc ctggcttttg
     7081 attggaacag ttcgctgcgg atggccgcat taagggaggc agagagggtg ttgcgatatt
     7141 taccgaaggt cccgtaaagg gtgctgtagg cgctctgccg aaccctgcgg tttttcgact
     7201 ggatgaagac ggcgtaacgt tcctctgaga atggggtttc ctctccgttt tcgcccacga
     7261 tgggtgggaa cgtcatatcc gcgttggtca gcatggaaaa aatagtctct ggcgcccgag
     7321 tcacctcgcc cgatgcagcg agaagagact cttctttttg ggaaaggata tgcttgcgct
     7381 tccgggatat ctcatccagg aaaaaggagt acatttcaag ccctttctct ctggccacca
     7441 atgcggatat ctcactgtcg gtcatggaca gaatctccgg aacgataaag gaacctgctt
     7501 ccgatgccat tacggcaagg gattccgctc tcgatgccat agattgaggg tcctgcttcc
     7561 ttgtatcttc gtggcttttc atgtgagcgt agacgtagat cctggaaacg gattctccga
     7621 tcctgtcaga caggcgcagg cattccagaa gattcgcgtg cgaggcagag atgttacccg
     7681 aaaaggatcc cagagagggt ataagggcca tggtctccgc gaaagaattt tcccaggcct
     7741 tgtcatcctc gaaaagatcg gacagtctcc acatgaaacg cgggtcaaga tcttccctct
     7801 cggggatatc tccggctgtt gttgttccct caagagtcat gctgaaatta ttcattaata
     7861 ttcctccata agagccgtta tcagaagggc tcgaaatttt tgtataggaa ggatccattc
     7921 cccagcatgg atgctaacac aagaatgggc ctaaacagaa actctgtgcc ggaaaggagc
     7981 agatgaaaaa gagcctccac gggaaattga caaataacta ttgacagacc ggtcagtcta
     8041 tgaaagaatt tactaaaccg actggtcggg tagtaggcca gtagctaaag ccgaaaggga
     8101 tgataacaat gaagagatcc ggatctttgg cactgtttgc tgttgttttt tgttttcttg
     8161 cgggtatcgt gtcacttgct gaggcggcca cggtgacctg gtggtatgat gttcaggtaa
     8221 ccgggacgag gtatcttttc aatccggcta ccggcaacgt cgatcagact cccctgtcat
     8281 cctcgggcct tgcaaagatt gagttcaatg acgcgatgcg gggaggtcca gttgtaattc
     8341 agactccagt cctgggttat accgagggga agagaaccgg ctggaatttt tcaggggaaa
     8401 gagcctacgc cgggatgctt ttcaagacat ccggggtttt tgctccgggg aacataagcg
     8461 ggacaattgt tgttgacgat ttatcaaagt caggaagcaa gggacattac gaataccagt
     8521 ttcacggagt caggaagaac tagatgcaat tacggaatag gggagagtga cgggagatga
     8581 gaaagacagc aattgcaaca tctgtggttc ttttgatact ggcatttgcg ggagcggttt
     8641 cggcggccga tatctcggga aactgggatg tcaaggttca cgggtacaac tatttcacgg
     8701 acaggacgcc ctccagggtt ccgatcgcgg agacggaaac cacaatgaag atcgagcagg
     8761 acg
//
LOCUS       DAOTIQ010000066         8622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_9816_length_8622_cov_7.203105, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000066 DAOTIQ010000000
VERSION     DAOTIQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9816_length_8622_cov_7.203105"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..744
                     /locus_tag="P5541_07825"
     CDS             136..744
                     /locus_tag="P5541_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV71931.1"
                     /translation="MSNALGTKEKIVQVARSQFARNGYHGASMDSIVKGTGLSKGAIY
                     WHFKGKKELFTAVLEAEAQKVISYLYPGPGDLDGGAVVFFVKRGEQYLDALWEDHELK
                     LIWLTLFIETQRGGEESRELSNVAGELLNRLHETLRPVVMEAFPGLRGGYGDMPLEKI
                     MLLVDIFFDGMVMNLGLRLDISQAKECWRFVVNQFINFEGKR"
     gene            747..2096
                     /locus_tag="P5541_07830"
     CDS             747..2096
                     /locus_tag="P5541_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRV71932.1"
                     /translation="MQQKKIFVLLLLVLSLFVLVLGRRAWAEEKEILSVEKAIELAFD
                     NNPLVGVAEKRVEQARARVREATASRAPVLSASALYQEVWKDPHYPVYIGGSATASGY
                     ALAGYRETWKTALTLSWLLYSSGSVENGIRASNLALEAVREESVRTIQAVEHAVRLAY
                     YELQRSRAREAVAEEAMALVKEHYRQAESLYRNGIVAKKEILRAKVAVSESELNLIRA
                     ANAVDVTFRALERAVGKTLQGEYPMPDPMDEPPAAELLADPKERALLNRPELRALSFS
                     ERSAKAAAASASGSKGPSIFIQGEAFSAGQTFYPDAVDDWKITAVAEWKLYDGGKAGA
                     KRDEALATAGELIHRMEDMRRQIALEVSVAELDLRSALQRLEVAKSQVDLAEEDYRMA
                     VARYTSQVGTAVDVLDASVSLSASKNQMVDAVYDTRKAWADMMFAIGKNIQTQEDKR"
     gene            2102..3148
                     /locus_tag="P5541_07835"
     CDS             2102..3148
                     /locus_tag="P5541_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV71933.1"
                     /translation="MKKTALLLVVVAVFSGLIVFRGYQQKQAQNLVPEEEAVKVEVVS
                     LKKMIMSEVVEFSATIEPEEKSAVVPRAPGRTVLQVFVSEGDRVKKGQALASLDASII
                     MQQLEEARTIYETALADHRRFESLYEDEVVSRQAADQVRARYMQALSAYEQARILTGY
                     HTITSPSEGVVAKRLIDPGDTAPQGPAFLIFRQDRVKAVGAVPERHYHGVKPGDRVLL
                     SVDALPGKDLEAEVTSISPVIDPATRTAKVEVILPSEGLVMPGMFARARISAGERESM
                     VLPLEAVSQMAGTGETTCYVASEGLARLRVVEAGIEQGGYVEITGGLQAGEEVIVTRS
                     RSVNDGTRIEVFRR"
     gene            3145..6258
                     /locus_tag="P5541_07840"
     CDS             3145..6258
                     /locus_tag="P5541_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV71934.1"
                     /translation="MKLPEISVKRPVMTLMVFLFVLVIGSISFRNLKLDLLPKIEPPV
                     MTVITPWPGASASDVEQRITKVMENSLSMLEGVDDIISKSLDNLSVVSVKFKWGSNLD
                     VRSGDVRDAVNFARRELPSDADESVVLRITSGTIPVIEITMTAERSYQGLYHFVDKSV
                     VEELSRVPGVGQILVFGGVQREIQVVLDAEKMEAWRIPAEAVAGVLERENLNIPAGSL
                     REGDTEYFIRIPGRFSSLDEIRNTVLGIHNGSPVKLGDVAEVRDGYKELTMNGWQGDK
                     ASVVLVVMKNSDANTIEVSRALLGRLEELKKGNFPSDVDYEIVMNTADFITNSISNLG
                     TSLIAGIVLVFLVTWAFLKRLPASLIVSGAIPFSLIITFIVMGRLDYTINIFTLSALA
                     MASGMVVDNAIVATDQIIFHIEQGSRRRIAAVLGASEIGSALLASTLTTVVVLLPLAF
                     LSGLVGVFFSALTIVMVIAVSASLFVSLTFIPMMGSKFFGREAGTLKIHELTEEFFAG
                     MEKAYSSLLSWSLENRKKVIASALLLLFLTFIGFRNIGTELTPDPDTGDISISFSLPE
                     GTRIETTDALVREVISYCKENIPEARVIFGMDGMEEGGFSVAVGQNAGPNIGTLGIKL
                     VDKRERDRSAFEIANEIRNWIRTRPGIEDMTVLVSSPIKAMFMGSKPLVIEIFGDDLQ
                     EVTLVAEDISALLSGIPGTADISVSRRQDRPEIWVDPDREKAAFLGVSTSAISRTARI
                     YFAGYETSEAFWEGENDYPIRVRLDEKQRNSRDIFEKLTVPGAGGVPVRLLSVAELRD
                     EAGPPQIERKNRQRYITVGANVHGRSLGEVTADASRMVGEMDIPEGIRVSFGGQIKEQ
                     SEAFRQMGLLVLLGIVLVYMVMAGQYEAYLDPFVILFSIPFALTGVVFAFLLTGLYIS
                     MQALLGMIMLIGIVVNIAIVLVDYINLVRARGAKLREAIVEAGHRRLRPAFMTTMTTF
                     FGMLPMALSRGQGAEIWRPLAVSVMGGLVVSMLVTMLLVPVVYSLVEEKIRKKPRFVE
                     AREAARS"
     gene            6255..6557
                     /locus_tag="P5541_07845"
     CDS             6255..6557
                     /locus_tag="P5541_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71935.1"
                     /translation="MRMLWIICSESIGEELMEKVLDVDGVEGYTVWRDVLGKDNKGNK
                     THWGDAVFPGLNWVFMIFGSNEMIDGLKERYRVFREEPYVKKAGIKAFIHDAEEVL"
     gene            complement(6565..8436)
                     /locus_tag="P5541_07850"
     CDS             complement(6565..8436)
                     /locus_tag="P5541_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRV71936.1"
                     /translation="MVFTLPQITILIVILSSMALFIHGRWRHDVIAMTALLVCVFTGL
                     VPGSEAFSGFGHPAVVTVACVLVLSCGLQITGAVSFVADRILPASAGPGLSILAITSL
                     GAFLSAFMNNVGALALLMPVGIQIADRNGIPPGKVLMPLAFGCILGGTTTLIGTPPNI
                     IISGFRSQAGLGSFAMFDFTPVGISIALSGILFIGLIGWRLVPEREQSDPESFETGAY
                     LSEVRVSEGSPVIGKRVREVEEMLDNADAQILGVVRGEIHFSAPGHSLKLREKDILVI
                     EAEPEGLSSSLSKLKLKLEEALTPKEAEETEEKKREICERYKQSCPPPDEDNGEKKKN
                     RVEDFIVREMVPAPGSGIDGLSATDIRLRSRFGVNLLAISRKGYRSIRRLRTTPLHPG
                     DVLLLQGTSETLSDFASQYDCLPLAARDIRVPRSGEVLTASTVMASAVALAALGIFPA
                     AVAFAAGALAMVVLRIVPLRSVYTAIDWPVVILLGAMLPVAGAMASTGAADLMAGVLL
                     ETVARGNAVAALVVILTSTMLLTDFMNNAATAAVMAPIALSVALQLDVNPDTFLMAVA
                     IGASCAFLTPIGHQNNTLILGPGGFSFGDYWRMGLPTDILVILTATPMLLLVWPLQI"
BASE COUNT         2068 a         2144 c         2506 g         1904 t
ORIGIN      
        1 ttgcctttcc tgtctcccgt atcctgtatc ctgccgcctg gttttccgat tcacgatttc
       61 tcaatattga cagaccgttc ggtctatgtt atccttcctt cgattcgcag actgttcggt
      121 ctggaggggg tacatatgtc caatgctttg ggaacaaaag agaaaatagt acaggttgcc
      181 cggagccagt ttgcccgcaa tggttaccac ggagcaagca tggattccat cgttaaaggt
      241 acaggtctta gcaagggtgc catctactgg catttcaagg ggaaaaagga acttttcacg
      301 gcagtgctgg aagcagaggc acagaaggtt atcagttacc tttacccggg tccgggtgac
      361 ctcgatggcg gagctgtggt gttttttgtc aaaagagggg agcagtatct tgatgctctg
      421 tgggaggacc acgaactcaa actgatctgg ctgaccctgt ttatcgagac ccagaggggg
      481 ggcgaagaga gcagggaact ttcgaatgtt gccggagaac tcctgaacag gcttcatgag
      541 actctccgcc cggtggttat ggaggccttt cccggtctcc ggggtggcta cggggatatg
      601 ccgttggaaa agatcatgct gctggtcgat atattctttg acggcatggt tatgaacctt
      661 ggtctcaggc ttgacatatc ccaggccaaa gagtgctggc gatttgtggt aaatcagttc
      721 atcaattttg aagggaaaag gtgagtatgc aacagaaaaa gatctttgtt ctgctattgc
      781 tggtcctgtc cctctttgtc cttgtccttg ggagaagagc ctgggccgaa gagaaagaaa
      841 tactgagtgt tgaaaaggca attgaacttg ctttcgataa taaccccctc gtcggtgttg
      901 ccgaaaaacg ggtagaacag gcccgggcac gggtcaggga agccactgct tcccgtgcgc
      961 ccgtcctttc ggcttcggcg ctgtatcagg aagtatggaa ggacccccat taccctgtct
     1021 atatcggagg atccgctact gcaagtggtt atgcactggc gggctacagg gagacatgga
     1081 agacagccct taccctatca tggcttctct acagcagtgg cagtgtcgag aacgggataa
     1141 gagcctcgaa cctggcgctg gaggcggtcc gtgaggaatc tgtcagaaca atacaggccg
     1201 tcgagcatgc agtaaggtta gcttattacg aactccagcg ttcacgtgca agagaggcag
     1261 ttgccgaaga ggccatggct cttgtgaagg aacactatag acaggccgaa tctctgtaca
     1321 ggaacggaat tgtcgccaaa aaagaaatac ttcgcgcaaa agtagcggta tcagagtctg
     1381 aactgaacct cataagggca gccaacgctg ttgatgtaac cttcagggcc cttgaaaggg
     1441 ccgtcggaaa gacccttcag ggcgagtatc cgatgcccga cccgatggat gaaccacccg
     1501 cggcagaatt gcttgccgac cccaaggaga gagccctttt gaaccgtccg gaactgcggg
     1561 ccctgtcgtt ctcggagcgt tccgccaaag ccgctgccgc atcggcgtcg ggatcaaagg
     1621 gcccctctat ctttattcag ggcgaggcct tctcggcggg acagactttt tatcccgatg
     1681 ccgttgatga ctggaaaatc accgctgttg ccgagtggaa actctacgac ggaggaaagg
     1741 ccggggccaa aagggacgag gcactggcga cggccgggga actgatccac aggatggagg
     1801 acatgagacg gcagatagcg ctggaagtat ccgtggcgga actggacctg cggtcggctc
     1861 ttcagcgtct cgaggtagcg aagtctcagg tcgatctcgc tgaggaggac taccgcatgg
     1921 cggtggccag gtatacctcg caggtcggta ccgcggtcga tgtcctggac gcaagtgtct
     1981 ccctttccgc atcaaaaaac cagatggtcg atgccgtcta tgacactagg aaagcatggg
     2041 cggacatgat gttcgccatt ggaaagaata ttcagaccca agaggataaa aggtgatcgg
     2101 gatgaaaaaa acagctttat tactggttgt ggttgcggtt ttctcgggat tgatcgtctt
     2161 caggggttac cagcagaaac aggctcaaaa ccttgttcct gaagaagaag cggtaaaagt
     2221 tgaggtcgtt tctctcaaaa aaatgatcat gtcggaggtg gtggagtttt ctgccactat
     2281 agagcctgaa gagaaatctg ccgtggtccc aagggctccg gggagaacgg tcctccaggt
     2341 ctttgtctcc gaaggggaca gggtgaaaaa gggccaggcc ctggcgtcac ttgatgcaag
     2401 cataatcatg cagcaactgg aagaagcccg gacgatctac gaaactgccc tggccgatca
     2461 ccgtcgtttc gaatccctgt atgaggatga agtggtgagc cgtcaggcgg cggaccaggt
     2521 cagagcccgt tatatgcagg ccctttcagc ctacgagcag gccagaattc tgaccggcta
     2581 tcacaccatt acctcccctt cagagggcgt ggtggcaaaa cggcttatag atcccggcga
     2641 taccgctcct cagggccctg ctttcctgat ctttcgtcag gacagggtca aggctgtcgg
     2701 tgccgttccc gaaagacact accatggggt caagccaggc gacagggtcc tgctttcagt
     2761 ggatgccctc cctggaaagg accttgaggc tgaagtcaca agcatttcac cagttatcga
     2821 ccccgcaacc aggacggcaa aagtggaggt gatcctgcca tcagaaggtc tggtgatgcc
     2881 cggaatgttt gccagggcca ggatcagcgc cggggagagg gagtcgatgg ttctccccct
     2941 ggaggcagtc agccagatgg caggcaccgg tgaaactacc tgctatgttg ccagcgaagg
     3001 tctggcgcgg ctaagggtcg tcgaggcagg gatcgagcaa ggtgggtacg tggagataac
     3061 cggaggcctt caggccggtg aagaggttat cgttaccaga tccaggagtg tgaatgatgg
     3121 aaccagaata gaggtgttcc gcaggtgaaa ctgcctgaga tatctgtaaa aagacccgta
     3181 atgaccctga tggtatttct tttcgtcctt gttatcgggt cgatctcatt ccggaacctc
     3241 aaactggacc ttctgccaaa gatcgaaccg ccggtaatga cggtcattac accgtggccg
     3301 ggtgcttcgg caagcgatgt tgagcagaga ataacaaagg tgatggagaa cagcctctca
     3361 atgcttgagg gtgtcgacga cataatatcc aaatctctcg ataatctctc tgtggtatcg
     3421 gtgaagttca aatggggttc caatctggat gttcgttctg gcgatgtcag ggacgcggtc
     3481 aattttgcga gaagggaact gccatccgat gcggacgagt cggtggtcct gaggataact
     3541 tccggtacaa ttccggtgat agagataacc atgaccgccg aaaggtccta ccagggactt
     3601 tatcactttg ttgacaagag cgtcgttgag gaactctcca gggttcccgg tgtgggacag
     3661 atcctggtct ttggaggtgt ccagagggag atccaggtag tcctggatgc agaaaaaatg
     3721 gaagcctgga ggattcccgc cgaagctgtt gccggtgttc tcgaaaggga gaacctcaat
     3781 atacctgccg gttccctccg ggagggtgac acggaatatt tcatccgtat tcccggaagg
     3841 ttttcgtctc ttgacgagat aagaaacacc gtgttgggga tccataacgg aagtccggtc
     3901 aaactcggtg atgtcgccga agtcagggac ggctacaagg aactgacaat gaacggttgg
     3961 cagggtgaca aggcctcggt ggtccttgtg gttatgaaaa acagtgatgc caacaccatc
     4021 gaggtctcaa gagctcttct tggaaggctc gaagaactga aaaaggggaa ctttccctcc
     4081 gatgtggatt acgagattgt catgaacact gcggatttca taactaactc catctccaac
     4141 ctcgggacat cactgatagc gggtatagtt ctggtcttcc ttgtcacctg ggctttcctc
     4201 aagaggctgc cggcatcgct tatagtttcc ggggccatcc ccttctcgct gataatcaca
     4261 ttcatcgtta tggggcgtct tgactacacg ataaacatat tcaccctttc ggcacttgcc
     4321 atggccagcg gaatggttgt cgataacgcc atagtggcca ctgaccagat aatattccac
     4381 atagaacagg ggagcaggag acgcattgcc gccgtccttg gcgcaagtga gataggttcc
     4441 gcacttcttg cctctaccct cacgaccgtg gttgttcttc ttccgctggc attcctttcg
     4501 ggacttgtgg gagtgttctt ttcggcactc acgatagtaa tggtcattgc cgtgtcggct
     4561 tcactatttg tgagtctgac cttcataccc atgatgggga gcaagttctt tggccgtgaa
     4621 gcaggcaccc tcaagataca cgaactgacc gaagaatttt tcgccggcat ggagaaggcc
     4681 tactcatcgc tcctctcatg gagccttgag aacagaaaaa aggtaatagc ctccgccctc
     4741 ctcttgcttt ttctgacctt tatcggtttc aggaacatag ggacggaact gacacccgat
     4801 ccggatacgg gtgatatctc catatctttc tcccttcctg aggggaccag gatagagacc
     4861 accgacgccc ttgtcaggga ggttatatcc tactgcaagg aaaacatccc tgaggcaagg
     4921 gttattttcg gaatggatgg catggaggag gggggctttt ccgtcgccgt gggacagaac
     4981 gccggaccca atatcggtac ccttgggata aaactggtgg acaagaggga aagggatcgt
     5041 tcggcattcg agatagccaa cgaaatacgc aactggatcc gtaccaggcc cgggatagag
     5101 gatatgaccg ttcttgtcag ttcgccgatc aaggcaatgt tcatgggctc caagcctctt
     5161 gtcatagaaa tattcggaga cgatctgcag gaagttaccc tggttgcgga agacatttcc
     5221 gccctgcttt ctggcatccc cggtaccgcg gatatctccg tgagccgcag acaggacaga
     5281 ccagagatct gggtcgatcc cgacagggaa aaagcggcgt ttcttggagt tagcacttcc
     5341 gcaatatcgc gcactgccag gatatatttc gctggctacg agacgagcga ggccttctgg
     5401 gagggcgaaa acgattaccc catccgggtg cggctcgacg aaaaacagag gaacagtcgg
     5461 gacatctttg aaaaactcac cgttcccggg gctggaggtg tccctgtccg actgttgtct
     5521 gtggcagagt taagggatga agcaggccca ccccagatag agcgcaagaa cagacagagg
     5581 tatatcacgg tgggggcaaa cgtacacgga agatccctgg gcgaggtgac agctgatgct
     5641 tcaagaatgg tgggagagat ggatataccc gaggggatca gggtctcctt cggagggcag
     5701 ataaaagagc agagcgaggc cttccggcag atgggacttc ttgtgctgct gggaatagtt
     5761 ctggtctaca tggtaatggc cggacaatat gaggcttacc tggatccctt tgttattctc
     5821 ttcagcatac cctttgccct tacgggggtc gtctttgcct ttctgcttac cggcctctat
     5881 atttccatgc aggctctcct gggaatgata atgctgatag gtatagtcgt gaatattgcc
     5941 atcgttcttg tggactatat caatctcgtc agggcaaggg gggcaaagtt gcgggaggct
     6001 atcgttgaag cgggacatcg ccgtctcaga ccggctttca tgaccaccat gacgaccttt
     6061 ttcggcatgc tccccatggc gttgagcagg gggcagggag cggagatatg gcgcccactt
     6121 gctgtctcgg taatgggagg acttgttgtg tcgatgcttg tgaccatgct gcttgttccc
     6181 gtggtatata gccttgtcga ggagaagatc aggaagaaac ccagatttgt ggaggccagg
     6241 gaggcggcac gttcatgagg atgctatgga taatttgcag cgaaagcata ggagaagaac
     6301 tgatggagaa agtccttgac gttgacgggg tcgagggcta caccgtatgg cgagatgttc
     6361 tgggcaagga taacaagggc aacaagaccc actggggaga cgcggtattc cccggattaa
     6421 actgggtctt catgatattc ggatccaacg aaatgataga tggtctcaag gaaagataca
     6481 gggtcttcag agaagaacca tatgtaaaaa aggccggcat aaaagccttc atccatgatg
     6541 ccgaggaggt cctttagaca tttttcagat ctggaggggc caaaccagaa gaagcatggg
     6601 ggttgccgtc aggatgacaa gaatgtccgt aggcagcccc attctccagt aatcgccgaa
     6661 ggagaacccg cctggtccca ggataagggt gttgttctgg tgccctatgg gtgtaaggaa
     6721 agcgcatgaa gctccgatgg ctactgccat aaggaaagtg tcggggttta cgtcaagctg
     6781 gagagccacg ctcagggcaa tgggagccat gacggcggct gttgccgcgt tgttcatgaa
     6841 gtcagtcagc agcatcgtag atgtcaggat cacaacaagg gcagcgacgg cgtttcccct
     6901 tgctacggtc tccagcaaga ctcctgccat aagatccgcc gcgccagtag atgccatggc
     6961 acccgcgacg gggagcatag ccccgaggag tatgaccacg ggccagtcga tggcggtgta
     7021 gaccgaacgc aagggaacaa ttctcaggac caccatggca agggctccgg cagcgaaagc
     7081 gacggccgcc gggaagatcc ctaaagcggc caacgcaacc gcggaagcca tcacagtaga
     7141 tgcggtcaga acctcaccgc tcctgggcac ccttatgtcc ctggcggcca ggggaaggca
     7201 gtcgtactgg gaggcaaaat ccgaaagagt ctccgaagtg ccctgcagaa gcagtacgtc
     7261 gccgggatgg agcggggttg ttctgaggcg ccttatggac ctgtatcctt tgcgcgaaat
     7321 ggccagcaga tttacgccga acctggacct cagcctgatg tccgtagcgg aaagaccatc
     7381 gataccggag ccgggggcag gaaccatttc acgtactata aaatcctcga cccgattttt
     7441 tttcttctca ccgttatcct cgtccggtgg tgggcatgat tgcttgtacc tctcgcagat
     7501 ctcgcgcttc ttttcttctg tctcctccgc ctctttgggg gtgagtgctt cttcgagttt
     7561 cagtttgagt ttggaaagag aggaagagag tccctccggt tcggcctcta tgacaaggat
     7621 gtccttttct ctcagtttaa gagaatggcc aggagcagaa aaatgaatct cccccctgac
     7681 cacaccgagg atctgggcgt cggcattgtc aagcatctcc tcgacctccc gtacccgctt
     7741 gcctatcacg ggactaccct cggagactcg tacctcgctt aaatatgccc ccgtctcgaa
     7801 actttcgggg tcagactgct ccctctccgg gacaaggcgc caaccgatca gtccgatgaa
     7861 caggattccc gataacgcta tggaaatacc aacgggggtg aaatcaaaca tggcaaaaga
     7921 accaagccct gcctggctcc ggaatccgga gatgataatg ttcggagggg tcccgatcaa
     7981 ggttgtcgtt ccccccagta tgcagccgaa cgcgaggggc atgaggacct tgccgggagg
     8041 tatcccgttc ctgtccgcta tctgtattcc cacgggcata aggagggcca aagcccccac
     8101 gttattcatg aacgctgaaa ggaaggcacc gagggaggta atggcaagaa tgctcaagcc
     8161 tggacctgcg gaggcgggga ggatcctgtc ggcaacgaaa ctcacggccc cggtgatctg
     8221 gaggccgcaa ctcaggacaa ggacacaggc caccgtaacg acggcaggat gcccgaaccc
     8281 cgaaaaggcc tcgcttccgg ggacgaggcc tgtgaaaacg cagaccagaa gagctgtcat
     8341 ggcaataacg tcgtgccgcc acctcccatg gataaacagg gccatggaag aaagaattac
     8401 tatcagaatg gtgatctggg gaagggtaaa aaccacgata tcctccaccg tattcccaaa
     8461 ggaagaataa attgtttcac cggggattca tggctccccg atgcgaagtt tgagggatta
     8521 ttctgtccgg gtcaagttga caactcgatt tcccacgata taatacttga gcgttccgcc
     8581 ggcgtagctc agttggtaga gcagctgact tgtaatcagc ag
//
LOCUS       DAOTIQ010000067         8433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_10124_length_8433_cov_8.085462, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000067 DAOTIQ010000000
VERSION     DAOTIQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10124_length_8433_cov_8.085462"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..702)
                     /locus_tag="P5541_07855"
     CDS             complement(52..702)
                     /locus_tag="P5541_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HRV71937.1"
                     /translation="MKEHEKDRKNGEYGEEGTPGKHDSHNSHESHPIIPLRGDYRTLL
                     SFQKAEVVYDITFRFAHRFLSRGDRTVDQMIQSARSGKKNVLEGSKAGRTSKETEIKL
                     TNVGRASLEELLDDYLDFLRARDMRLWNKDCKEALFVRELGRKEPQTYELYRKFVETR
                     SAEVVANIAICLIHQANYLIDQQLIRLEQDFIKDGGLRERMTRTRLAARNGNKSAR"
     gene            1134..2072
                     /locus_tag="P5541_07860"
     CDS             1134..2072
                     /locus_tag="P5541_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRV71938.1"
                     /translation="MNDNRLTLPIMAALGAVYFFWGTTYLGMKFAIETLPPLIMLGIR
                     FITAGAIMFAWKWIRGDVGTTWRQWRGAAMVGGLMLLGGTGGVAWSEMEIPSNIAAIL
                     VATVPLWIALIRWIALRHDRPGKVATAGLLLGFAGIILLVRSTPASDAASQEYGISLV
                     VLMVAPFLWATGSLVSRVVDLPRSPALAISMQMLTGGLFCLMAGLAMGEGSLVHLEAI
                     SLKSAAALAYLVTFGSIVGFSSYIWLLKKTDPALASSYAYVNPVVAIALGWVLAGESL
                     NAGSLLAAGVILLAVVLIVRGTARPATVKEIKPLPE"
     gene            complement(2136..2549)
                     /locus_tag="P5541_07865"
     CDS             complement(2136..2549)
                     /locus_tag="P5541_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV71939.1"
                     /translation="MGSNGDKAFAGRLEDLPVFPANDHSGTQKRVVFGPDVFWNDHVM
                     RHFTVEAGSSSPFHSHDWPHYVVILEGSAKAMIMGKTWEVSAGSWAFVPPNTEHFFEN
                     IGKTDLKFLCIVPKKATRTGWTIKGAAEASKIAPR"
     gene            complement(2691..3560)
                     /locus_tag="P5541_07870"
     CDS             complement(2691..3560)
                     /locus_tag="P5541_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71940.1"
                     /translation="MRKPANLLVVIAILLCALPAAADHSTSGTVSGPTPYVYTIKCDP
                     GEGFLVEVTSSQPTSVNILSMTPDSRADGGWAFNAVKSSQKAYSHLMDYRAPSGEPKN
                     NASHWHYRVSILASTYEPADFTLSVTLLGDGGSGDSGKDAKERLDELAKNLNREWDAL
                     EEKIKAMDWLKDTVKELNERKAALLEKKGEIDRITALAKSESDPQEKQRLLDIRNGLV
                     TEYKTGAAQYNEDYRKIEGDLEVRNLMVRRQNAVHDLGVALKDPYNRKDYAACIAIAN
                     GSEIARELGWAVE"
     gene            complement(3624..4712)
                     /gene="rsgA"
                     /locus_tag="P5541_07875"
     CDS             complement(3624..4712)
                     /gene="rsgA"
                     /locus_tag="P5541_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRV71941.1"
                     /translation="MKNTDMENMGLSEGFIAESDRYAGLFVGRVVSQSRSLYRVATDR
                     AEVQSEVSGKFRFGTGSMSGYPAVGDFVMVDRNNNAEGNAVIHHVLQRKSAFIRRAAG
                     TSNEDQVVASNIDTVFICMSLNRDFNLRRVERYLGIAWDSGAVPVIVLTKADLCGDLT
                     EKLSELEAVALGVDVIVTSSLAEDGYEPVRKYLGRGKTVAFIGSSGVGKSTLINRLLG
                     RDLILTRDIRKDDRGRHTTTRRELIVIPGGGVVIDTPGMRELGIESADLARTFIDIDE
                     LSAQCRFHDCTHKGEPGCAVRRAIDDGLLSEDRLESYLKLKKEAKYNGLNSRQIETEK
                     FSAMFAEVGGMKNARKFAKEKNRRKQGY"
     gene            complement(4895..5092)
                     /locus_tag="P5541_07880"
     CDS             complement(4895..5092)
                     /locus_tag="P5541_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HRV71942.1"
                     /translation="MSGKERIIIRPGLRVKIVQKQHQGTGELTEGIVRDILTKSSTHP
                     HGIKVRLEDGTVGRVKYVKEE"
     gene            complement(5152..5607)
                     /locus_tag="P5541_07885"
     CDS             complement(5152..5607)
                     /locus_tag="P5541_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71943.1"
                     /translation="MIQYRTIFPALILFLIFAGSAAAVMPPEHYARMAENSRIRATAV
                     VDSVEVLETTKESTRKKVVFSLRHDFGMEVPEIFSGICFSVDWPWQKPMAGGTIYFYP
                     EKGDLVFVTVAADGGHITSYTYLDENLETLFVGNPDRIRFGMGNAHLEL"
     gene            complement(5671..6294)
                     /locus_tag="P5541_07890"
     CDS             complement(5671..6294)
                     /locus_tag="P5541_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRV71944.1"
                     /translation="MDQSTVHVYVFDSISDWEYGYAAAGINNPQFQISPGRFRVSTVA
                     LKKTPVTTMGGIRIQPDLTLDQVSPDDSAMLILPGGTSWDQGQETEAVEVARAFLDAG
                     VPVAAICGATAGLAGGGLLDNRKHTSNAPEYLAATGYKGASLYQDAPAFTDDNLITAS
                     GIAPVDFARHIFKRLEIFKPEVLDAWFGLFKTGRAEYYFALMQAMGA"
     gene            complement(6403..7275)
                     /locus_tag="P5541_07895"
     CDS             complement(6403..7275)
                     /locus_tag="P5541_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRV71945.1"
                     /translation="MKPLRVRLRQASKLQGIPQHVIEKDYALSYILAGISRHPALKDS
                     LVFKGGTALKKMFFGKYRFSEDLDFSCKNAPTGDELERCIAEALEYARSLLMEQGPFS
                     FLLERYLERDPHPHGQEAFTIRTQFPWHPEPLCRVKMEISHDEPVLIEPARRALIHDY
                     YGETLQSEVTCYRLEEIIAEKLRTLLQTHAKLVTRGWNRPRSRDYYDLWQILKKFGGD
                     FLPGRVIEILGPKNAHRNVFFQITEDFFSKELVSEASRNWENSLGAFVIDLPEFDQVM
                     QELKPLIHQVLKGS"
     gene            complement(7272..8129)
                     /locus_tag="P5541_07900"
     CDS             complement(7272..8129)
                     /locus_tag="P5541_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRV71946.1"
                     /translation="MRRAKENGKGENYRIRFLKSLVEDGLTVFSVSDARRIAEESGIP
                     RTYINRLLSFLVRDGWITRLKRGLFARIGPLSGDIQIHPFAIATHMIEPSAISHWSAL
                     HHHGLTEQVPGIVTAITPKKIVTPSMRLDRSVESTNRRHGWLVEGVRYEYRSVKEEHY
                     FGVEEIWVDQYSRIPIMDRERAALDLFVSPGIFGGMGEVLGIMDEHLASLDVERLVGY
                     ACRYGKIAPAKRLGWILERSSVPHKILEPLLAIPAAGYLLLDPSGQNEGIFEKRWNIR
                     VNLGSGRIE"
BASE COUNT         1827 a         2409 c         2058 g         2139 t
ORIGIN      
        1 tgaatctgaa aaaatggcag gtctggaaca ccaaaaaatg acgagggaac actatctggc
       61 agacttgttc ccatttcgcg cagccagccg ggttcgggtc attcgttcgc gcagcccgcc
      121 gtctttaata aaatcctgct ccaggcgaat aagctgctgg tcgataagat agttggcctg
      181 gtgaatcagg cagatggcga tatttgccac cacctcagcg gaccgggttt cgacaaactt
      241 acggtaaagc tcgtatgttt gaggctcttt tctccccagt tcccttacaa aaagcgcttc
      301 cttgcagtcc ttgttccaga gccgcatgtc gcgggcgcgt agaaaatcga ggtagtcgtc
      361 aagcaactct tccagactgg cgcggccgac gtttgtcagc ttgatttccg tctcttttga
      421 cgtgcgcccg gccttgcttc cctcaagaac atttttcttt cccgatcttg ccgactgtat
      481 catctgatcg acagttcggt cacctctgga aaggaatcta tgggcaaagc gaaaggtaat
      541 gtcgtagacg acctcggctt tttggaaaga taacagagtc ctgtagtcgc cgcgcagtgg
      601 aataatggga tgggattcat gggaattatg agaatcatgc tttcccggcg ttccctcctc
      661 cccatattcc ccattcttcc tgtccttctc atgctccttc atcaaggcta ccccctgctc
      721 ttttcctgcg ccacggctct atcgtttgct ggcctttgat atagctgaag tttcttcctg
      781 agcaaaaaat ttttactgag ggttttacca ttttgttaca aaccagaacc tcgccagggg
      841 tttatccgga agccaaccag ccttttccga aaacctcaac ccaggctgat ttcaaaatca
      901 ttgaatttca ttccttgata acatacaaca tgggaaggca ataaacaata agggctctgg
      961 agaaatccgg atccagagcc ttctcagaat acctgatgag cagtgcgacc tggtccgact
     1021 atcctgtatt gtaggcgttt ctttgttcat tgtatttatg ggatatgcgc tatgatattt
     1081 caccggacag gagataactg acccgaaagt gaaatttgaa aggagatcca tcaatgaacg
     1141 acaacagact gacacttccc ataatggcag ccctgggcgc cgtctatttt ttctggggaa
     1201 caacctacct tgggatgaag ttcgccatag agacgctacc gcccctgatc atgctgggga
     1261 ttcgctttat taccgccgga gccattatgt tcgcctggaa atggatccgg ggggatgtcg
     1321 gcaccacatg gcgccagtgg agaggggctg caatggtcgg ggggctgatg ctgctcggcg
     1381 gaacgggtgg agtggcatgg tcggagatgg agataccttc caatatagcg gctattctcg
     1441 ttgccacagt tccgctctgg atagccctta tccgctggat agccctgcgg catgacaggc
     1501 ccggcaaggt cgccacggcc ggccttctgc tcggattcgc cgggataata ttgcttgtcc
     1561 gctcaacccc cgcatcggac gcagcttcgc aggaatatgg catcagcctc gtcgtcctta
     1621 tggttgcgcc cttcctgtgg gctacggggt cgctggtttc gcgcgtcgtg gacctgccca
     1681 ggtctccggc cctggcgata tccatgcaga tgctcaccgg agggcttttt tgcctgatgg
     1741 ctggtctggc gatgggcgaa ggctccctgg tccaccttga ggccatctca ctcaaatctg
     1801 ctgcagccct ggcctatctt gtcactttcg gctctatcgt cggattcagt tcctatatct
     1861 ggctcctcaa gaaaaccgat ccggccctgg cttcgagtta cgcctacgta aacccagtag
     1921 tcgccattgc acttggatgg gttcttgcag gagagagcct gaacgcggga agccttctgg
     1981 cagcaggagt catcctgctg gcggtcgttc tcatcgtgcg gggtacggcc agaccggcga
     2041 cagtaaagga gatcaaacct ctgcccgaat agaccaggca gcccggcatt taaggctgcc
     2101 tggtccttct tcaggttttt cctttccgca atgcctcaac gaggcgcgat cttagacgcc
     2161 tcagcagctc cctttattgt ccatccagta cgtgtcgcct tttttggaac tatgcagagg
     2221 aatttgagat cggttttacc gatattctca aagaagtgct ccgtattcgg cggcacgaac
     2281 gcccacgatc ccgccgaaac ctcccaggtc tttcccatga tcatggcctt tgctgagcct
     2341 tccaggatca ctacgtaatg gggccagtcg tgcgagtgaa aaggcgaact tgatcccgcc
     2401 tcgaccgtga agtgacgcat cacgtgatca ttccagaata cgtccggacc gaaaacgacc
     2461 ctcttctgag tcccgctgtg atcattcgcc gggaaaaccg gcaaatcttc gagccttccc
     2521 gcaaaggcct tatccccgtt tgatcccata aaaaccatcc tttcgtcatg cttcgtctga
     2581 atctcttaac ctgcagggat ctggacggat ggctaaatga gccctacgtt acgccactct
     2641 tgatacccga aactgaagag ttgcgaccgg ttctgcggta aaaaggttgg ttattctacc
     2701 gcccagccaa gttcccgggc tatctccgat ccgttggcta tggcgataca ggcggcgtaa
     2761 tcttttctgt tgtaggggtc cttgagggcc actcccaggt cgtgaaccgc gttttgcctc
     2821 ctgaccatca aattgcgaac ttccaggtct ccctctatct ttcggtaatc ctcgttgtat
     2881 tgggctgcac ccgtcttgta ttctgttaca agcccattcc ggatatcgag aagcctctgt
     2941 ttttcctgag gatcggactc gctttttgca agagcggtga tcctgtctat ctctcccttt
     3001 ttctccagga gtgctgcttt tctttcgttc agttccttca ccgtgtcctt caaccaatcc
     3061 atggccttta tcttttcttc cagggcatcc cactcccggt tcagattctt tgcaagttca
     3121 tccagtcgtt ccttcgcgtc cttgccggaa tcaccggaac ccccgtcacc cagaagggtt
     3181 acagaaaggg taaaatccgc gggttcgtat gtcgaggcca ggatcgagac cctgtaatgc
     3241 cagtgagaag cgttgttctt gggctccccc gagggagctc tgtaatccat taggtgagag
     3301 taggctttct ggctgctctt cacggcattg aaggcccaac ctccgtcggc gcgggaatcg
     3361 ggcgtcattg aaaggatgtt caccgaggtt ggctgacttg acgttacctc cacgaggaaa
     3421 ccttccccgg gatcgcactt gatggtgtag acatagggag tgggtcccga tactgttccc
     3481 gatgtcgaat gatccgccgc cgcgggaagc gcgcacagaa ggatcgcgat aacgacaagc
     3541 aagtttgcgg gttttctcat tatttcatct cctcgctgtc tatgtttagg gtcgccttga
     3601 ggcaacaagg cgacccctac gtgtcaataa ccctgctttc tcctgttttt ttcttttgca
     3661 aacttcctgg cgtttttcat tccgccaact tccgcgaaca tggcagaaaa cttctcggtc
     3721 tctatctgcc ttgagttcag gccgttgtac ttcgcttcct ttttcagctt caggtaactc
     3781 tcgagcctgt cttcggatag cagcccgtca tctatcgccc ttctgactgc acagcccggc
     3841 tcacccttat gagtacagtc gtggaacctg cactgcgccg acagttcgtc gatatcgata
     3901 aaggttcttg ccagatccgc gctttcgatg ccgagctctc tcatcccggg ggtgtcgatg
     3961 acgacccctc cgccggggat gacgatcagt tcccgcctcg tcgttgtatg ccggcccctg
     4021 tcatctttcc tgatatcccg ggtcagaata aggtcccggc cgaggagcct gtttatcagg
     4081 gtcgacttgc cgacacccga tgagccgata aaggcgaccg ttttccctcg gccgagatac
     4141 ttgcggacag gttcgtatcc gtcttcggcc aggctggaag tgacgatcac gtcaactcca
     4201 agagcgacgg cctcgagctc ggacaatttc tccgtcagat caccgcacag gtccgctttt
     4261 gtcagaacga tgaccgggac ggccccgctg tcccaggcta tccccaggta gcgctcgacc
     4321 ctgcgaaggt tgaagtccct gttgagggac atgcagatga acaccgtgtc gatattcgag
     4381 gccacgacct ggtcttcatt cgacgtcccc gccgcccttc tgatgaaggc gcttttccgt
     4441 tgaaggacat gatggatgac agcattcccc tcggcattat tattccggtc gaccattacg
     4501 aaatctccca ccgcagggta accggacatg gatcctgtcc cgaaacggaa ctttccggag
     4561 acttcagatt gcacctccgc ccgatccgtc gccaccctgt aaagactcct ggactgggaa
     4621 acgacgcgcc cgacaaaaag acccgcatat ctatcagact cggcgatgaa accctcgctg
     4681 agccccatgt tttccatatc tgtatttttc aattcgataa cctccaaaag ttttttattt
     4741 caacttttgg gaggcatata cattatcgtg ccctgtatgc acctccccgc cctgtcacaa
     4801 tatccactgc acagttgata aacataaatc cagtctcctt ccagaatcag gttggatatt
     4861 caaggacaat cctagcacaa cctatttgcg cgctttattc ctcttttaca tatttgaccc
     4921 ggcccacggt accgtcctcg agcctcacct tgatcccgtg ggggtgggtg gagcttttgg
     4981 tcagaatgtc tctgacgatg ccctcggtga gttctcccgt cccctggtgc tgcttctgta
     5041 cgatctttac cctgagcccg ggcctgatga taatcctttc cttacctgac acaacaaatc
     5101 cccctgtcga gtgcaggccc gaacataacc tggcacgttt ttgcaaaatg atcagagttc
     5161 gaggtgtgca ttccccatcc caaagcggat cctgtcgggg tttcccacga agagggtttc
     5221 caggttttcg tccaggtagg tatagcttgt aatatggcca ccgtcggccg ccacggtaac
     5281 gaagaccagg tccccttttt cggggtagaa atagatcgtg ccccccgcca tcggtttctg
     5341 ccatggccag tccaccgaaa aacagatgcc tgaaaaaatt tcgggaactt ccatgccgaa
     5401 gtcgtgcctc agggagaaga caactttctt tctggtactc tctttggtgg tctcaaggac
     5461 ctcaaccgaa tcaacaacag ccgtcgccct tatcctggaa ttttcggcca tcctggcgta
     5521 atgctcgggc ggcatgaccg ccgccgcgga acccgcgaaa atgaggaaaa ggatcaaggc
     5581 aggaaagata gtccgatact ggatcattaa caacccctcc tttttggctc tgagaggact
     5641 cccccaacgc gtcgtcggga acagtaacga ttacgctccc atcgcctgca tcagtgcaaa
     5701 gtaatactcg gcgcgccctg tcttgaagag tccgaaccag gcatccagga cctcgggctt
     5761 gaaaatctcc agccgtttga agatatgccg ggcaaaatcg acgggcgcga tccccgatgc
     5821 cgtgatcagg ttgtcgtcgg taaaggctgg cgcatcctgg taaagggaag cccccttgta
     5881 ccccgttgcg gcaagatact ccggggcatt gctcgtgtgt ttccggttat ccagaagacc
     5941 gcctcccgcc agccccgccg ttgccccgca gatcgcggcc acggggaccc ctgcatccag
     6001 aaatgcgcgg gcaacttcca cagcctcggt ctcctgcccc tggtcccatg aagtcccgcc
     6061 gggaaggatc agcattgcgc tgtcatcggg ggaaacctgg tcgagggtga ggtccggctg
     6121 gatgcggatc cctcccatgg tcgtgaccgg ggttttcttc agggccacgg tacttacacg
     6181 gaatcgtccg gggctgatct gaaactgggg gttgttgatc cccgccgccg catagccgta
     6241 ctcccagtcc gaaatgctgt cgaaaacata tacatgaacg gttgactgat ccatggtctg
     6301 ctcactccct atcgctttcc ttttgtcgaa aatttagcac atggtcactt tgcttcggct
     6361 tttatcaggg accaccattg acccccacag gattattctg gactaagatc ctttgaggac
     6421 ctgatggata agaggcttta gttcctgcat gacctggtcg aattcgggca ggtcaataac
     6481 aaaagcgcca agactattct cccagtttcg cgaggcctcc gaaacaagct ctttactgaa
     6541 gaaatcttct gtgatctgaa aaaacacgtt tctatgggca ttcttgggac caagtatctc
     6601 aatcactctc ccgggaagga aatcccctcc gaacttcttg agtatctgcc acagatcata
     6661 ataatccctg gaacggggtc gattccatcc cctggtaacc aatttcgcat gggtttgcaa
     6721 taaggttctc aatttttcag cgatgatctc ctcaaggcga taacaggtca cttcgctctg
     6781 aagggtctct ccataataat cgtggatcag agcacgcctt gccggctcga tcagaacagg
     6841 ctcatcatgg ctgatctcca tttttactcg acataatggc tcggggtgcc acgggaactg
     6901 ggtccttatg gtaaatgctt cctggccatg tggatgagga tcacgttcca gatatctttc
     6961 cagcaaaaac gaaaacgggc cttgttccat gagcaggcta cgagcgtatt caagtgcttc
     7021 tgctatgcac ctttccagtt catcgcccgt tggggcattt ttgcaggaga aatccagatc
     7081 ttcagaaaac ctgtatttcc cgaagaacat ctttttcagt gctgttccac ccttgaaaac
     7141 cagagaatcc ttcagggccg gatgcctgga tattccagcg agaatgtaac tcaaggcata
     7201 atccttctct attacatgtt gcggaattcc ctgtaatttg gaagcctgac gaagccggac
     7261 tcgaagcggc ttcattcgat cctgccgctt ccaaggttga cccggatgtt ccaccgcttt
     7321 tcgaaaattc cctcattttg ccccgatgga tcaagcaaaa gatatccagc ggcaggaatt
     7381 gccagaagag gctcaaggat cttatgagga acggatgatc gctccaggat ccatcccagt
     7441 ctcttggctg gcgctatctt tccgtatctg catgcgtaac cgacaagcct ttcaacgtca
     7501 agtgaagcaa gatgctcatc cattattccc agcacctctc ccattccgcc aaagatcccg
     7561 ggcgaaacga acaaatccag ggctgccctc tcccggtcca tgatggggat cctggagtat
     7621 tggtcgaccc agatctcttc taccccgaaa taatgttcct cttttacgct cctgtactca
     7681 tatcggactc cttcaaccag ccatccgtgt cgacggttgg tactctcaac ggatctatcg
     7741 aggcgcatgc ttggggtgac aattttcttt ggggttatgg ccgtgacgat accgggtact
     7801 tgctctgtca acccgtgatg gtgcagagcc gaccagtgac ttattgcgga aggttcgatc
     7861 atgtgtgttg ctatagcaaa gggatgtatc tgaatatcgc ctgataacgg cccgatacgc
     7921 gcgaacagac cccgcttcag gcgggtgatc catccatccc ttaccaggaa ggacaggagc
     7981 ctgttgatat atgtcctggg gatcccgctc tcttctgcta tccgccttgc atcggaaaca
     8041 gaaaacacgg taagaccatc ctcaacaagt gacttgagaa acctaatcct gtaattttct
     8101 cccttgccat tttctttcgc tcgtctcatt tgaaaagctc cattataaat taaactgcaa
     8161 aaacaccaaa taatggtatt tatgcagttc attttataac ggaatcaggc catttgcaac
     8221 ccgttgatct tgcaggccta tcacctgtcg attagtacat gcagttccgt ggtcacaggg
     8281 aacccgtccc ggacggcaaa agccagttca ttctccgctg cgatatggct ctccctgttt
     8341 acgttacaga aaagacacgc cccaccggcc ccgcatcacc gcgccatcag ggcgaacaca
     8401 ccccagccca tgtattcacg ggtgtacctg gcg
//
LOCUS       DAOTIQ010000068         8231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_10465_length_8231_cov_7.231287, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000068 DAOTIQ010000000
VERSION     DAOTIQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10465_length_8231_cov_7.231287"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1271)
                     /locus_tag="P5541_07905"
     CDS             complement(285..1271)
                     /locus_tag="P5541_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71947.1"
                     /translation="MLSLVRSGSRGLFLRIAEPKVMKALETGFSLTEKGYDDALNEAG
                     EGQTLLFVPPCGMMRGEPRVFLADCGPGEILAFLLNARLAEGVARARLLPRTIIFRVS
                     GEYGAMLEQMARDYGARVGKIPSFLKWRDNGRTAVFFTASSLNRPIAMKDLFPDVLFV
                     KMPYEKLLRSLRSRAMTYFNEARGHADWRNLEIRLYDSWERYDLQVRRLRMILEEMEV
                     GLLLGEGWGKDYAHVLMPVPVYRFHLATFLEPEKIKEILMGLEYCLSGERLVDLDLYE
                     GKKKNSWGALARKEEDRQMAGVRYRNKLAERLTPRTRKMLAALEKELEKAEN"
     gene            complement(1295..1912)
                     /locus_tag="P5541_07910"
     CDS             complement(1295..1912)
                     /locus_tag="P5541_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV71948.1"
                     /translation="MLARVLRIIEGLVPGMTLPVSLDDFRGRCILHVSDTPSVFYGDL
                     RRLVDHFCPVFLVHTGDLADDVKVGLHPGEMGLYRKRLKALFHAIGGVPAGHIAIACG
                     NHDIAEDIRKVCKGCLLFEGGGRATICGLDIALGHYIREIPSPPSRFNLYGHDLSSPP
                     PGIREIFLNGVTAVNLIDTETGEIAAIPYPRYVDESRLCRRKRGL"
     gene            complement(1921..2394)
                     /locus_tag="P5541_07915"
     CDS             complement(1921..2394)
                     /locus_tag="P5541_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV71949.1"
                     /translation="MAKAAKSVEKEVMEVLETSLRSIWESDSETYRKTAADDCSFFEW
                     YISPQRIDGLDFHIRELRVHRSVVGGGADPLEEDEQLFEHEILQPRVQVYGECAIVTF
                     TLMVRAVFPGKIVHKSHNETRVFHNFGTKKKPDWKLVHCHKSPIATPESLSVLRT"
     gene            2538..2711
                     /locus_tag="P5541_07920"
     CDS             2538..2711
                     /locus_tag="P5541_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV71950.1"
                     /translation="MAKAVVDKDTCVGCEACVGTCPVSAISMEDGKAHVDPDTCVECG
                     ACVAVCPVSAISQ"
     gene            2895..3266
                     /locus_tag="P5541_07925"
     CDS             2895..3266
                     /locus_tag="P5541_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRV71951.1"
                     /translation="MLRIIPIRVSRIIWTSGIAAFLLISVIPLGRTPLQDVSGGDKIA
                     HFAVFFLLAIFPILTGAARTRTVTIILLLLAFGSEALQCLTSYRSGEIGDIAADISGL
                     AVGILSGIALTGKKPPQEPTL"
     gene            3785..4555
                     /locus_tag="P5541_07930"
     CDS             3785..4555
                     /locus_tag="P5541_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRV71952.1"
                     /translation="MTRTIAVINQKGGVGKTTTAANLGVALARSGKNVLLVDADPQAH
                     LSVSLGHPDAEPSLYEVLTGKGSLQDVAVSVEKGVDLVPADITLARADMELSWQPGKD
                     FILREVLASASERDFTLIDCPPSLGLLTLNALTASDEVLIPLQAEFLPLRGISQLIEA
                     IDLVRSRTNPGLSITGVLFTRWDGRKVLSRETLEQAGTCFGNALMKTRIRECVSLAEA
                     PGNGQHIFDYAPRSNGASDYLDMAHEVLAMNPAGRARN"
     gene            4555..5235
                     /locus_tag="P5541_07935"
     CDS             4555..5235
                     /locus_tag="P5541_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71953.1"
                     /translation="MAKKRIGEAIPPSRRNKLEGMIGEGREKPAGGYREALETSSRAL
                     AMMAGLLADMARGSSEMNGELLKCCGELAKQLEDMGFLPVRGTSHATGEEAAPSSTVK
                     IPGPVAAAPPNTRHSTGDDRKTTFLKTVETIALNRHNLQEALDALGMASGTGVAETGR
                     VFAEREPFVRDSDSWKKMLSNLAALEKETGFKNLRSMADQYFSKDPDPGRERGIENFL
                     SETRSRGR"
     assembly_gap    5323..5422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5423..6096)
                     /locus_tag="P5541_07940"
     CDS             complement(<5423..6096)
                     /locus_tag="P5541_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV71954.1"
                     /translation="MTFDAFSGSGQTTPAREETFRSVFFEVGGQVFAFGVEMVREILL
                     WQGARKVPHTHKALMGVATVRGEVIPVIDLGVFLGVGSTLPGEQKKLVVLEIEGGIKA
                     AFAVDEVRRIFDTELSRVDRTLKGVVLGDYLDCVIKHPDGDILMPDPVKIMEALRLET
                     ARLSRFAAGEPIAEDETVKESGPADVKAKKQEIVFHDGPSPFRSGEQKEAEKQDKSDK
                     PDKPDKP"
     gene            complement(6142..6927)
                     /gene="flgG"
                     /locus_tag="P5541_07945"
     CDS             complement(6142..6927)
                     /gene="flgG"
                     /locus_tag="P5541_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRV71955.1"
                     /translation="MIRALWSAATGMKAQQTNLDVIANNLANVNTSGFKKQRADFQDL
                     VYQIDREPGAPVEPGSTVPTGVQVGLGSRVAGTGRMMGQGSAQVTENPTDIMIQGPGF
                     FQVILPDGSTAYSRNGAFQIDGQGQFVTTDGYLLEPSIAVPEDAVELTVSDTGIVSVK
                     IPGDVLFQEIGQIELARFVNPPGLLAQGKSLFLETDASGAPMTAYPGEDGMGTIIQGA
                     LEMSNVQVVEEMVAMITAQRAYEAGSKAVKTADDMLAIANSMK"
     gene            complement(6950..7393)
                     /locus_tag="P5541_07950"
     CDS             complement(6950..7393)
                     /locus_tag="P5541_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71956.1"
                     /translation="MLSSGASMVFEGLPLETKLAEETGARASGRAAGTGSTGSPFEGK
                     LLEAEMGDLLDRLDTAAGRLFRFPSGSVLSEYRAVVGQILLRAGEMMEIRRDFPARSG
                     KAGFLVQRVRKGLEELEDILGREGRRSRIMGITAEIKGCLLSMTA"
     gene            complement(7482..>8231)
                     /locus_tag="P5541_07955"
     CDS             complement(7482..>8231)
                     /locus_tag="P5541_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71957.1"
                     /translation="QGFVYFPRVEGPFRVFLERGSGPGLAFEFPEDRENRIRKETAQK
                     TMVETGSASGGGAVNGSPQEPAYGDEIKKIRDEQKRETERLNARIRELAAERDRLRSE
                     LDSTLAAMARERNIEVDLAPRPLVSAQPHGIIPEPVHEEAPEPGTGMSREEAALSFLN
                     AWKSGDLEGMYRLLSPETRARIPDVNALAEYMGNKVLPDRMPGDAKLVDKGEGGVKIH
                     FAVKVLVMRTLRSVNLELVQGNGGFSVGLAD"
BASE COUNT         1726 a         2407 c         2195 g         1803 t
ORIGIN      
        1 aacggtgaac ggaaaaggca gaaggtcctg tcattctgag gaccaacggg acgaagaatc
       61 tgggtcagaa ccagatcctt cgtcacttcg ctcctcagga tgacaacaaa agcaaaaggg
      121 gcctttgccc ctgcttccct gcctccagca tcccgtctcc ttggtcttcc gccccccacc
      181 gttgaccgtt gaccgttgac cgtttaccgt tcacgcactt gatctttttg cttcacgatt
      241 cacgaaaaaa cagatccttc gctgcgctca ggatgacagc aaatttagtt ctcggccttt
      301 tccagttcct tttccagggc ggcaagcatc ttccgggtcc ggggggtcag cctttccgca
      361 agtttatttc tgtacctgac tcccgccatc tggcggtcct cttctttcct ggccagagct
      421 ccccaactgt tcttcttttt gccctcgtaa agatccaggt ccaccagccg ctctcccgac
      481 aggcaatact cgagtcccat gaggatttcc ttgatcttct caggctcaag gaatgtcgca
      541 aggtggaaac ggtagacggg cacgggcata agcacatggg catagtcctt tccccacccc
      601 tcgcccagga ggagtccaac ctccatctcc tccaggatca tcctgagcct tcgaacctgc
      661 aggtcatatc tctcccacga gtcgtagagg cgtatctcca ggtttctcca gtcggcatgc
      721 cccctggcct cgttgaaata ggtcatggca cggctcctga ggctgcgcaa caatttttcg
      781 tagggcattt tcacgaaaag aacatccggg aaaagatcct tcatggctat tggcctgttg
      841 aggctcgacg cggtgaaaaa gacggccgtc ctgccgttgt cgcgccattt caggaaagag
      901 gggatctttc cgaccctggc accgtaatct cttgccatct gttccagcat ggccccgtat
      961 tccccgctga cacgaaaaat gatcgtcctg ggcaggaggc gagccctggc caccccctcg
     1021 gcaagcctcg cgtttagcag gaacgccagt atctccccgg gaccacaatc cgccaggaac
     1081 actcttggct cccccctcat catgccgcag ggaggaacaa aaagcagggt ctgaccttct
     1141 ccggcctcgt tcagggcatc atcgtaaccc ttttccgtga gggaaaatcc ggtttcaagg
     1201 gccttcatta cctttggttc ggcgatcctg aggaaaaggc cccgggatcc gctcctgaca
     1261 agagaaagca tccttcagac tcccctttcc actttcacag gccccgcttg cgcctgcaaa
     1321 gtctggactc gtcgacgtag cgcgggtatg gtatcgccgc gatctctccc gtttcggtat
     1381 ctatgaggtt gacggcggtt acaccgttaa ggaagatctc ccggattccg gggggagggg
     1441 aagaaagatc gtgaccgtag aggttgaacc tggacggggg agaaggtatc tcccggatgt
     1501 agtgaccaag ggcaatatca agaccgcaga tagttgcgcg ccccccacct tcgaagagaa
     1561 ggcaaccttt acagaccttc cggatatctt cggcaatgtc atggttgccg caggcgatag
     1621 caatatgacc ggcggggacc ccgccaatgg cgtggaagag cgccttgagc cttttcctgt
     1681 aaagccccat ttctccgggg tgcagaccca ccttgacatc atcggcaaga tctcccgtgt
     1741 gaacaaggaa caccgggcaa aaatggtcga ccaggcgcct tagatcgccg taaaagaccg
     1801 aaggagtatc ggagacatga aggatacacc ggcctcggaa atcgtccagg ctcaccggca
     1861 gggtcatgcc gggcacaagt ccttcgatga tgcgcagaac ccttgccagc atcgtcccgc
     1921 ctaggtcctg agcacgctca ggctctcggg ggtggcgatg ggcgacttgt ggcagtggac
     1981 cagtttccag tccggcttct tttttgtgcc gaagttgtga aagacccggg tctcgttatg
     2041 gctcttgtgg acaatcttcc ccgggaatac cgccctgacc atcagggtaa aagttacgat
     2101 ggcgcactct ccgtagacct ggacacgggg ctgcagtatc tcgtgctcga ataactgctc
     2161 atcctcctcg agaggatcgg cccctccccc gaccactgac ctgtggaccc gtaactccct
     2221 gatgtgaaag tcaaggccgt cgatcctctg gggagatata taccactcga agaaggagca
     2281 gtcatcggca gcagtctttc tgtaggtctc ggaatcggat tcccagatac tcctgagact
     2341 cgtctcaagg acctccatga cctctttctc cacgctcttt gcggcttttg ccatgggccg
     2401 gacaccacct tttcgaaaac aatttcaccg agaaccatta taacggacaa ccggttgagc
     2461 agaggggtaa tttgcgctag aatgaacaga gtcaatttta cgatcgaagc aaaaccccaa
     2521 agaggaggga cttttgaatg gcaaaggcag tcgtcgataa ggatacctgt gtgggttgcg
     2581 aggcgtgcgt aggcacgtgc cccgtttccg ccataagcat ggaagacggc aaggcccacg
     2641 tcgaccccga cacctgcgtt gagtgcggag cctgcgttgc ggtatgcccg gtgagcgcga
     2701 tctcccagta gtttcaggag atcaggattg cggttgtgag ggcacttccg aaaggaaggg
     2761 cccttttttt gtcctcgtac ggagccataa aaccataccg aacaggtctt agtcagtaaa
     2821 ggtaaatcgt tccatcgcct tttagacaat gtctcagatg gctcaaaagg actcctgaaa
     2881 ggagatgatc ccgaatgctg agaataattc ctatccgggt ttcacggata atctggactt
     2941 ccggaattgc ggcgttcctg ctgatatctg tgatacccct gggccggaca ccgctccagg
     3001 acgtcagcgg cggagacaag atagcccact tcgccgtctt cttcttgctg gctattttcc
     3061 cgatattgac cggggcagcc aggacacgga cggtaacgat cattctcctg ctcctcgcct
     3121 tcggttcgga agcccttcaa tgccttacca gttacaggtc aggagaaatc ggggatattg
     3181 cggcagatat ttccggtctt gccgtcggca tcctttcggg tattgcttta accgggaaaa
     3241 agccccccca agaaccaact ctttgactga aaccgggaat cctcatccag tcttgcggct
     3301 taaatttgca agaccataaa gttatgatct actcttgtga tgtttgttta cttatctgct
     3361 ttacaggctt tatgctttgg tgttgtgata tttttgcatt tgtgtttttg gttttttatt
     3421 caccgggaca cagggaaacc cctttagcgc gcttaatgat ttacttagaa tgattttatg
     3481 gtaagatata cgttaatttt ttccacactc tgttgttctg taaaaaaacg gttttttgtt
     3541 tcgaaaaagg gggccatggc ccttttttca acaaaaaagt gaactttaaa gtgccccgaa
     3601 gcggtaaaaa tgctcgtaga tgggcccaga cgagtcctct ttgccaaaat ggatatgtgt
     3661 atagcctcag gaccatttaa caccctcaga gggcttctgc ggcagtattg tttgggtacc
     3721 gtcatcagga gtcaatgtga tattctccgc tggatggccg ataacccaag ggggggtgct
     3781 gtaaatgacc agaacgatcg ccgtgatcaa tcagaagggc ggtgtgggca agacaaccac
     3841 tgcggcaaat ctcggagttg cccttgcaag atcgggaaaa aatgttctcc tcgtggacgc
     3901 cgacccgcag gcacatttga gcgtgtccct gggccatccg gacgcggagc ccagcctgta
     3961 cgaggtcctg acggggaaag ggtcgctcca ggatgttgcc gtcagtgtag aaaaaggagt
     4021 ggatcttgtt ccagcggata taacccttgc gagggccgac atggaacttt cctggcagcc
     4081 cggaaaggat tttatcctcc gcgaggtcct tgcctcggca agcgaaaggg atttcacact
     4141 gatagactgc cctccctcgc tgggacttct gaccctcaac gccctcactg catccgatga
     4201 ggtcctgatc ccactccagg ccgagtttct ccccctgaga gggatctcac agcttataga
     4261 agccatagac ctggtcagga gccgcacaaa cccagggctt tccattacgg gcgtgctctt
     4321 tacccgctgg gacgggagga aggtcctctc cagggagacc cttgagcagg caggaacctg
     4381 tttcggcaac gccctgatga agacccggat aagggagtgc gtgtcactgg cggaggcacc
     4441 cggaaacggc cagcatatat tcgactacgc gcccagaagc aacggggcat cggattacct
     4501 cgatatggcc cacgaggtcc tcgcaatgaa cccggcaggg agggcaagga actgatggca
     4561 aaaaaacgca taggagaggc gataccccca tcacggagga acaagctgga aggcatgatc
     4621 ggggaaggac gcgagaaacc cgccggtgga taccgtgaag cactggagac atcgtcgaga
     4681 gcgctcgcga tgatggcggg gctcctggcc gatatggcac ggggttcctc ggaaatgaac
     4741 ggggaactgc tgaaatgctg cggagaactg gcgaaacaac tggaggacat gggtttcctc
     4801 cccgtaaggg gcacatccca cgcgacagga gaagaggcag cgccatcttc gacagtgaag
     4861 atccccggac cggtcgcggc agcgcccccg aacacccggc attccaccgg ggatgaccgg
     4921 aaaaccacgt tcctgaagac cgtcgagacc atagccctga acaggcacaa cctgcaggag
     4981 gcacttgatg cgctgggtat ggcaagcggg accggagtcg cggaaacagg cagggtattc
     5041 gcggaaaggg aaccttttgt cagggactcg gattcatgga agaagatgct gtcaaacctc
     5101 gcggccctgg aaaaggagac aggtttcaaa aacctgcggt cgatggcgga tcaatatttc
     5161 agcaaggatc cggaccccgg ccgggagagg ggaatagaga acttcctctc ggaaacccgg
     5221 agccggggca ggtaaaagcg cgaaaaaacc agaaacgacg gggatttact ccccgggctt
     5281 ctttttcccc ccgcccgatc cagacgcggg ggtttttttg ccnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnggtttatc aggtttatcg ggtttatcgg atttatcctg
     5461 tttttcggct tccttctgct cccccgacct gaatggtgat ggcccatcat ggaaaacaat
     5521 ttcctgtttt ttggccttta cgtccgcggg gcctgactct ttgacggttt catcttcggc
     5581 gatcggctcc ccggcggcga aacggctgag tcttgcggtc tcaagcctga gagcctccat
     5641 gatctttacc ggatcgggca tcaggatgtc gccgtcagga tgctttatca cacagtccag
     5701 gtagtccccg aggaccactc ccttgagggt acggtccacc ctggagagtt ccgtatcgaa
     5761 tatcctcctg acctcgtcca cggcaaaggc ggccttgatg cccccctcta tctccagaac
     5821 gacaagtttc ttctgctcac cgggaagggt gctcccgaca ccgagaaaga cccccaagtc
     5881 tatcacgggg atcacctctc cccttacggt ggcaacaccc atgagggctt tatgggtgtg
     5941 ggggaccttc cgggccccct gccagagcag tatctccctg accatctcaa ccccgaaggc
     6001 aaaaacctgt ccccccacct cgaagaagac gctcctgaag gtctcctccc ttgcgggggt
     6061 cgtctgcccc gatcccgaaa aagcgtcaaa ggtcatatcc tgcacggcaa tctctcctgt
     6121 cgagggcaca tgacggccct gttatttcat cgagttcgcg atggccagca tatcatcggc
     6181 tgtcttgacg gccttggagc ctgcctcgta tgccctctgg gcggttatca tggcaaccat
     6241 ttcctcaacg acctggacgt tgctcatttc cagggcgccc tggatgatcg tacccattcc
     6301 atcctcgccc gggtaggccg tcatgggcgc gccgctggcg tcggtctcga ggaagaggct
     6361 ctttccctgt gccagaaggc ccggcgggtt gacgaacctt gccagttcga tctgccctat
     6421 ctcctgaaaa agaacatcgc ccggtatctt cacggatacg ataccggtat cactcacggt
     6481 caactcaacg gcatcctcgg gcaccgctat tgagggttcc agcaggtaac cgtcggtggt
     6541 gacgaactgg ccctggccat cgatctggaa ggccccgttg cgggaatagg ccgtgctgcc
     6601 gtcgggaagg atgacctgaa agaaccccgg accctggatc attatatcag tcgggttctc
     6661 cgtcacctgg gccgagccct ggcccatcat cctcccggtt cctgccaccc tgctccccag
     6721 gcccacctga acacccgtag ggactgtgga gccgggctct acgggagccc cgggttcccg
     6781 gtctatctgg tagacaaggt cctggaaatc ggccctctgt ttcttgaaac ccgaggtgtt
     6841 gacatttgcc aggttgttgg ctatcacatc caggtttgtc tgctgtgcct tcattcccgt
     6901 cgcggccgac caaagagccc ttatcatcga agatcactcc cccgtttttt catgccgtca
     6961 tggacaagag gcatcccttt atctccgcgg taatgcccat tatcctgctc cttcgcccct
     7021 cgcggcccag gatatcctcc agttcctcca gaccctttct gaccctctgg acgaggaatc
     7081 cggcctttcc ggacctggcc gggaaatccc gtcggatctc catcatctca ccggcacgga
     7141 ggagtatctg ccccacaacg gcacggtatt cggataggac gctcccggaa ggaaaccgga
     7201 aaagtctccc ggcggcggta tcaagcctgt cgagaagatc tcccatctcg gcttccagga
     7261 gtttgccctc gaaggggctt cccgtggaac cggtgcctgc cgcccgtccg gaggcacggg
     7321 cgccggtctc ttctgccagt ttcgtctcca atggcaaacc ttcgaagacc atcgaggccc
     7381 cggaactcag catgggtcag caacacctcc agtgggcggc ccctcgcggg gacttcgcac
     7441 tttcagtatc ggcacccggg gcggttcact tgatggccgg gtcaatccgc caggccgacg
     7501 gaaaaaccac cgttcccctg gaccagttcc agattcacgc tcctgagggt acgcatgaca
     7561 agaaccttta cggcaaagtg gatcttgacg ccgccctccc ccttgtcgac aagttttgcg
     7621 tccccgggca tgcggtccgg caacaccttg tttcccatgt attcggcaag ggcgttcaca
     7681 tcggggatac gggccctcgt ctcgggtgaa aggaggcggt acatcccctc caggtccccg
     7741 cttttccatg cattcaggaa acttagggcc gcttcttccc tggacatgcc cgttccgggt
     7801 tccggagcct cttcatgaac cggttccggg atgatcccat ggggctgcgc cgagaccagc
     7861 ggcctggggg caaggtccac ctctatgttc ctttcacgcg ccatggcggc gagggtgctg
     7921 tcaagctcgc tccttagcct gtccctctcg gcggcaagtt ctcttatccg cgcattgagc
     7981 ctttcggtct ctcttttctg ttcgtcccgg atctttttta tctcgtcacc gtaagccggt
     8041 tcctggggcg acccgttcac ggccccgcct ccagatgctg aaccggtctc aaccatagtt
     8101 ttctgagccg tttccttcct tatgcggttc tcacgatctt cgggaaactc gaaggccagc
     8161 ccgggaccgg aacccctttc cagaaaaacc ctgaaggggc cctcgaccct gggaaagtag
     8221 acaaaaccct g
//
LOCUS       DAOTIQ010000069         8016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_10837_length_8016_cov_6.229996, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000069 DAOTIQ010000000
VERSION     DAOTIQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10837_length_8016_cov_6.229996"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="P5541_07960"
     CDS             complement(<1..556)
                     /locus_tag="P5541_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRV71958.1"
                     /translation="MDRTRIADPSLAPEGKKKILWAWQRMAVLSELEKKYLDEQPLSG
                     ITVGACLHLEAKTACLLLTLQKLGATVAAAGSNPLSTQDDVCAALVEEGVRVFSRRGM
                     TAKEYGENLRSVLDHRPGVIIDDGADLVVMVHEERPETLGEIYGGSEETTTGVKRLKA
                     MERENILAFPMISVNDALSKYLFDN"
     gene            complement(589..1587)
                     /gene="mtnA"
                     /locus_tag="P5541_07965"
     CDS             complement(589..1587)
                     /gene="mtnA"
                     /locus_tag="P5541_07965"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRV71959.1"
                     /translation="MLPESIRWDRKAGRLMILDQTLIPNETRYVECATCEEVARCIES
                     MKTRGAPAIGIAAAYGLVLAASGGIEGVMKGADRLASTRPTAVNLFWALERMKKYARE
                     KTGTGDLRDLLLKEADLILEEDIEANRNIGKNGQVLLPESCSVLSHCNAGALATGGYG
                     TALGVLRAARDAGKNVTVFLDETRPLFQGSRLSAWELMMDGFDVTVICDGMAAWLMKS
                     RGVDAVIVGADRIASNGDFANKIGTYGLAVAAKEHSVPFYVAAPSSTIDSSIKSGEDI
                     PIEERKEEEIRLLPGGMRLPEGVGVWNPAFDITPADLVTAHITERGLFRPPFSFHI"
     gene            complement(1603..2808)
                     /gene="xseA"
                     /locus_tag="P5541_07970"
     CDS             complement(1603..2808)
                     /gene="xseA"
                     /locus_tag="P5541_07970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRV71960.1"
                     /translation="MPGIVTVDELTGYISLLFERNELLSFLQVKGELADLKRHTSGHV
                     YFSLTGKESRVSCVLFRSDAGKVPRWPRNGDEVLLEGRAGVYGPRGTYQVYARNIRPV
                     GEGAQARAKKELFQRLSLEGLFDPAKKRNFPKYPLKVAVVTSSSGAAVRDVLKVSLGR
                     FPQCDLVVVPVTVQGTGSPAEIVEGLRRAGRIHGAEAVMLVRGGGSREDLNPFDEEDV
                     VRAVRNCSLPVITGLGHQTDRTLSDLAADKDAPTPSAAAETLFPSRGQVLGEIAGYRD
                     LLVTMTGSSLRRRSDMLDRCGAAMAKSLDRSTLLPAESLVDRLFEKMSAATVKELATS
                     NHKLEKTASSLDALSPLRVLSRGYASCLGPDGKMLSSALSISPGEAIVVRFRDGSLSA
                     EVKGSMPLK"
     gene            complement(2796..3251)
                     /gene="nusB"
                     /locus_tag="P5541_07975"
     CDS             complement(2796..3251)
                     /gene="nusB"
                     /locus_tag="P5541_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRV71961.1"
                     /translation="MKAIIPQKRRRSREIALQILYSIEMRENGDPDQAIGLFPFESED
                     EEVGSYASRLIRGVYSRRNEIDAMVRRSIVGWRPERMVLVDRVAIRLALFESLVEKLI
                     PVPVAISEAVELAKRFGTDESGKFVNGVLGKIVREIEPCGNENDRQCQV"
     gene            complement(3270..3755)
                     /locus_tag="P5541_07980"
     CDS             complement(3270..3755)
                     /locus_tag="P5541_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71962.1"
                     /translation="MFYFWKSGENGDLWVSGEGLRKAFSERYPGGAECSEISLLGDKE
                     LLNVSFVLDRETDPDIKNRSENSIREFASGVGIRDVQTGWVSDPSSKDLVSTRGLFRN
                     PFLWAGTAWIFFAVFRMGISGTILATLFSASVFFLAALFTTDKGSDIRKKIRGLFEKN
                     A"
     gene            complement(3818..4288)
                     /locus_tag="P5541_07985"
     CDS             complement(3818..4288)
                     /locus_tag="P5541_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRV71963.1"
                     /translation="MSVAKKEQGLEPESGGEVLEGEGSVRIAEEVIAQIATQALMKVN
                     GVQPASPGLVANLRLGKKTSGGVRISIEEEAVPAVVVEAYITTKYGLRIPDVAWDVQE
                     SIKKTLEQFTGYNVKAVNVYVQGVYFEDEQSREKPKAKPQEPKTPKAPVPETGD"
     gene            complement(4333..4719)
                     /locus_tag="P5541_07990"
     CDS             complement(4333..4719)
                     /locus_tag="P5541_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71964.1"
                     /translation="MGAREKIAFLSGLLEGLSPSGEAEKKIYAGIIQALEALAEEIDD
                     HDEILKEHEEFIEDLSDYCEQLEDDMDRLETSSGDFGEYSEDDMEDGYESMECPECGH
                     MFFFSADDLEDDGTIQCPECGFMIQV"
     gene            complement(4737..5309)
                     /gene="efp"
                     /locus_tag="P5541_07995"
     CDS             complement(4737..5309)
                     /gene="efp"
                     /locus_tag="P5541_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRV71965.1"
                     /translation="MGQVVDTSNFHSGMKIIWQEGIWEIVDCQHHKMGRGGAIVRTKL
                     KNLESGSIVEHSFRSGERLERVIFEDKPAQFLYQDGENYVFMDMQNYDQIYIHKDTLA
                     GVSQFLKDNLEVELEMYEGRIMGIELPKSVELRIVDTSPGFKGDTVSGSGKPATLETG
                     FTLSVPMFVETGDVIIVDTRTGEYVERAKH"
     assembly_gap    5524..5623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5624..6960)
                     /locus_tag="P5541_08000"
     CDS             complement(<5624..6960)
                     /locus_tag="P5541_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HRV71966.1"
                     /translation="MKITRVAFILLFLSQILFAPGGSWADARQENADTLASSLPSIQG
                     VTLMQGGSGLAVMEVTGNLLPAPDILSTGDNVLVLLWSGTYLPSAYWEKAFNTPLVSS
                     VVLKQESDGVHMTVRSSRRLVLGRTVGDPPTPVMTVYLATPDTAEEVIPRSPVKTPTA
                     GDPLAMTTPVTLELRDTDLRDVFRMLAGMVNMNVIIDPSVPPLPVTLSLDQVPMSEAF
                     GYLMRMYDISYSMMGKSIIVGKKENLAKTMGMERTRSFHVAYADPKQIPALLQGISGV
                     SNIVVDERLRTVYVTASEEHLQEVASVLQRIDHPGRQVILQARIIEVSESGQDDVRSL
                     INAVYERWYATFSSAGGTVGYYEGHENVIYDPDRTKRDINIPESITFPNIADNALKFF
                     DASLQVLVSQDKAKMLATPSVATVDGMKATIKLVQDVKYISGKDEAGNPTYGDV"
     gene            complement(6957..7538)
                     /locus_tag="P5541_08005"
     CDS             complement(6957..7538)
                     /locus_tag="P5541_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71967.1"
                     /translation="MTSSDSWIGSAQELWRDLTEGERFPRLVKWILALVLVSGTVWSA
                     LVFSQMFSTMRGSDTPPPAANAATGDEIRRLDETAKGFRNAVTARTGSTQLAVLASTV
                     GRKPFMPPGAGDYLPETALEGAGTPMIWVKAVMIKGQDAAAVVDIEGFGDGVILKKGA
                     SFGDGRGKVVSITNDRVVVTWSGQKIDIPVDRQ"
     gene            complement(7541..>8016)
                     /locus_tag="P5541_08010"
     CDS             complement(7541..>8016)
                     /locus_tag="P5541_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71968.1"
                     /translation="GSVFLVVAFLAGQAVFSRIVTRERGQIEELRTRQRILELDIQNK
                     TRLLQAYKTTLGVIGEYRITLPMDEVAFFSSVERELSKGGIQVNSMKPAKAVAGNSAV
                     QVDFVGPYYTVLNVIADWRRMGTAVRMIGITLSRDEPGMVKGTVVLETVLAEGGA"
BASE COUNT         1735 a         2352 c         1879 g         1950 t
ORIGIN      
        1 tgttgtcgaa aaggtatttg ctgagggcat cattgacgga gatcatggga aaggccagta
       61 tattttccct ttccatggct ttcagccttt taacgccagt ggtagtctct tcggaccccc
      121 catatatttc tccaagggtt tcaggtcgct cttcatgaac catcacgacg agatcggctc
      181 cgtcatctat tatcacacca ggtctgtgat ccagaacgga cctgaggttt tccccgtatt
      241 ccttcgcagt cattcctctc ctgctgaaga cccgcacacc ctcttccaca agagcggcac
      301 agacatcatc ctgggtcgaa agcgggttgc ttccggcggc tgccaccgtg gctccaagtt
      361 tctgaagggt aagaagcaga cacgcagtct ttgcttcaag gtgcagacat gctccgacgg
      421 tgattcccga aagaggctgt tcgtcaagat atttcttctc cagttccgag agaacagcca
      481 tcctctgcca tgcccataat atttttttct tcccttcggg agcaagggag gggtcagcga
      541 tccttgttct gtccaatatt atttcctcct cgttgtttag aaaggtcgtc atatatggaa
      601 actgaaaggc ggcctgaaaa ggcccctctc ggttatatgc gcggtcacca ggtctgctgg
      661 cgtaatatca aaggcggggt tccagacccc gaccccttcg ggcagtctca ttccgccggg
      721 cagcagcctt atctcttctt cttttctttc ttctatgggt atatcttccc cggacttgat
      781 ggaagagtca attgtgctgg atggcgcggc gacataaaag gggaccgaat gttctttcgc
      841 ggccactgca agtccgtaag tcccgatctt gttggcaaaa tctccgttgg aagctatcct
      901 gtcagcaccg acgatgaccg cgtcgacgcc cctgcttttc atcagccagg ccgccattcc
      961 gtcacagatg accgtaacat caaaaccatc catcatgagt tcccatgccg aaaggcggga
     1021 accctgaaaa agaggccttg tctcatccag aaacacggtt acattcttgc ctgcatctct
     1081 tgccgctctc aacaccccaa gggcggttcc gtaaccccct gtagcaaggg caccggcatt
     1141 gcagtggctc aggacactgc aactttcggg aagcaggacc tgtccgtttt tgccaatatt
     1201 acgatttgcc tcaatatctt cctccaggat cagatcagcc tccttaagca gaagatcccg
     1261 aaggtctccg gtccctgtct tttcacgggc atattttttc attctttcaa gggcccagaa
     1321 aaggttgacc gccgtaggcc tcgtagatgc cagcctgtcg gcccctttca tcacaccctc
     1381 gatccccccg gaggcggcga gaacaagacc gtaagccgca gctattccta tggctggggc
     1441 ccccctggtt ttcatggatt ctatgcaacg tgccacctct tcgcaagttg cgcactccac
     1501 ataccttgtt tcattcggaa taagggtctg gtccaggatc atgagtcgcc ccgctttcct
     1561 gtcccatctg atcgactcag gaagcaccct tttcaactcc tttcacttaa gcggcatcga
     1621 acccttgact tcggccgaga gactgccatc gcggaaacga acaacaatag cctctcccgg
     1681 ggaaatcgaa agggccgaag atagcatttt cccgtctggg ccaagacatg acgcataccc
     1741 ccgggaaagg accctgaggg gagaaagtgc atcaagtgag gacgcggttt tttcaagctt
     1801 gtgatttgag gtcgccagct cctttactgt tgcggctgac attttttcaa agagacgatc
     1861 tacaagggat tccgcgggca aaagcgtgga tctgtccagg gattttgcca tggcggctcc
     1921 gcatcggtca agcatgtccg acctcctgcg aagtgaagat ccggtcatag taaccagaag
     1981 gtccctgtat cccgcgatct ctccaaggac ttgtcccctg ctggggaaaa gcgtctctgc
     2041 ggcagccgaa ggggtgggag catccttgtc cgcggccaga tcagaaaggg tcctgtccgt
     2101 ctgatgaccc aggcccgtga taacgggaag agaacaattt ctcacagccc tgaccacatc
     2161 ctcttcgtca aaggggttca gatcctccct gctgcctccg cccctgacaa gcataaccgc
     2221 ctctgctcca tggattcggc cggccctcct cagcccctcg acgatctccg ctggcgaacc
     2281 ggttccctga accgtcactg ggaccacgac caaatcgcac tgaggaaacc tgccgaggga
     2341 aaccttgaga acatccctga ccgcggcacc ggaagaggag gttaccacag caactttaag
     2401 ggggtatttg gggaagttcc tttttttagc ggggtcgaac agaccttcca gcgaaagcct
     2461 ctggaaaagt tctttttttg cccttgcctg ggccccttct ccaacagggc ggatgttcct
     2521 cgcatatacc tgataggtac ctctgggacc gtaaactcct gcccttccct ctaaaaggac
     2581 ctcgtcacca ttacgaggcc acctggggac ttttccggcg tcagagcgaa acaggacgca
     2641 ggaaaccctc gattccttac ccgtgagtga gaaatacaca tgtccgctgg tatgccgctt
     2701 gaggtcggca agttccccct ttacctgaag aaaggagagc aactcgttcc tctcgaagag
     2761 cagcgaaata taacccgtca gttcgtcaac agtaactata cctggcattg cctgtcgttt
     2821 tcgttcccgc agggctctat ctccctgacg atcttgccga gcactccgtt gacgaatttt
     2881 cccgattcgt cggtgccgaa acgtttggca agttcgacgg cttccgatat ggcaaccggg
     2941 acggggatca gtttctcgac aaggctctcg aataatgcaa gccttatggc aaccctgtcg
     3001 accagaacca ttcgctcagg cctccacccg acgatacttc ttctgaccat tgcatcgatc
     3061 tcgtttcttc gggaatatac tcccctgatc aaccttgaag cataggatcc gacctcttca
     3121 tcctcggact cgaaagggaa aagacctatc gcctggtctg gatcaccgtt ctcccgcatc
     3181 tcgatcgagt aaaggatctg aagggctatc tcccgtgatc tcctgcgttt ttgtggaatt
     3241 atagccttca attaaggatc ttccctctct caggcattct tttcgaatag gcctcttatt
     3301 ttttttctga tgtcgctccc cttgtcagtt gtaaaaagtg cggcaagaaa aaacacagac
     3361 gccgaaaaca gcgtagcaag tatcgtaccc gaaattccca ttctgaaaac agcaaagaat
     3421 atccatgctg taccggccca taaaaacggg tttcggaata atcccctggt ggagacaaga
     3481 tccttcgacg aagggtcact cacccacccg gtctgaacat cccttattcc aacccccgag
     3541 gcaaattccc tgatgctgtt ctcggaccgg ttctttatat ctggatctgt ctcccggtca
     3601 agcacgaaac tgacgtttag aagctccttg tcaccgagaa gggatatctc cgaacactcc
     3661 gccccgcctg gatatctttc gctgaaagcc tttcgaagcc cctccccgga tacccacagg
     3721 tctccgttct ctcctgattt ccagaaatag aacatttaca tcacctcata ataataaggg
     3781 gccggtcccc ctggaaccgg ccccggctga accggtatca gtccccggtt tctggaaccg
     3841 gtgccttggg ggttttgggt tcctgtggct tggccttggg tttctcccgg gattgctcgt
     3901 cctcgaaata aactccctga acatacacgt ttacggcctt gacattgtat cccgtaaact
     3961 gttcaagggt cttcttgatc gattcctgca catcccatgc cacatcgggg atcctgaggc
     4021 catatttcgt cgttatatag gcttcgacaa cgaccgcggg aacagcttct tcctcgatag
     4081 agatacgaac accgccggag gtctttttcc ctaatctgag gttggcgaca agacccgggc
     4141 tcgccggctg tactccgttt accttcatca atgcctgggt agcgatctgg gcaatgactt
     4201 cctctgctat tcttacgctg ccctcgccct ccaaaacctc tccgcctgat tcaggctcaa
     4261 gaccctgttc ttttttggct acgctcataa cctgtctccc tccctgcttt atggtgtcga
     4321 aatcggagcg gttcaaacct ggatcataaa gccgcattcc gggcactgga tagtcccgtc
     4381 atcttccaga tcgtcggcac tgaaaaagaa catgtggccg cactcagggc actccatgga
     4441 ttcataacca tcctccatgt catcctctga atattcgcca aaatctcccg aagaggtctc
     4501 cagccggtcc atatcatcct caagctgttc acagtaatcg gaaagatcct caatgaactc
     4561 ctcatgttcc ttaaggatct cgtcatggtc gtcgatctcc tcggccagag cctcaagggc
     4621 ctgaatgata cccgcgtaaa tctttttctc ggcttccccc gacgggctca gcccttcaag
     4681 aaggccgctg agaaaagcga tcttttccct tgctcccata tcctgactcc tccttttcag
     4741 tgctttgccc gctctacgta ttctcccgta cgggtgtcca caattattac gtctccggtc
     4801 tctacaaaca tgggaacgga aagggtaaaa cctgtctcaa gggttgcggg cttcccgctc
     4861 ccggagacag tgtcaccctt gaaaccggga cttgtatcaa cgatccggag ttcgacgctc
     4921 ttgggaagtt ctatccccat tatccgtccc tcatacatct cgagttcaac ctcaaggttg
     4981 tccttcagga actgcgaaac tcccgccaga gtgtccttgt ggatgtaaat ctggtcatag
     5041 ttctgcatat ccataaagac atagttctcc ccatcctggt aaaggaactg ggccggcttg
     5101 tcctcgaaga taaccctctc gagtctttcc ccggaacgga aggaatgttc cactatagat
     5161 ccgctctcaa gatttttgag ttttgtccgg actatggctc cgcctctgcc catcttgtgg
     5221 tgctggcagt ctacgatctc ccagataccc tcctgccaaa ttatcttcat tccggaatgg
     5281 aaattacttg tatcaaccac ctgacccatt caaataaccc tcccgaggcc cctgcccctt
     5341 tcatttcttt gagaacccgc gcggcaggtt cgtattgaga ctatcatttt aaccgccccg
     5401 gataccattg ttcaactgcg tatggcgtat tcagttgagt tttacatcac ttccgctgat
     5461 agcggattcg ggaacatcaa gaatataggg cacaactatc atgataacct cggatttggt
     5521 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacatcgc cgtaggtcgg
     5641 gttgccggcc tcgtccttgc cggaaatgta cttgacgtcc tggacaagtt tgatagttgc
     5701 cttcattccg tccacagtgg cgaccgaagg ggtcgcgagc atcttggcct tgtcctggct
     5761 tacaagtacc tgcaggctgg catcgaaaaa cttcagcgcg ttgtcggcaa tgttcggaaa
     5821 agttatgctc tcgggaatgt tgatatccct ctttgtcctg tccgggtcat aaataacgtt
     5881 ctcatgccct tcgtaatagc caactgttcc tccggcgctg ctgaaagtgg cataccaacg
     5941 ttcgtaaaca gcgttgatca ggctccttac gtcatcctgg ccgctctcgc tgacctcgat
     6001 tattctcgcc tggagaataa cctgccttcc cggatgatct atcctctgaa gtaccgacgc
     6061 cacctcctgg agatgctcct ccgaggctgt gacgtaaacc gtcctgagcc gctcgtccac
     6121 gactatgttc gatacccccg atattccctg taaaagagcg ggaatctgtt ttgggtcggc
     6181 ataggccaca tggaaggatc tggtccgttc catgcccatt gttttcgcaa ggttttcctt
     6241 cttgccgacg attatggatt tacccatcat cgaataggaa atatcgtaca tcctcatgag
     6301 gtatccgaaa gcctcactca ttggcacctg gtcaaggctg agcgtcaccg ggagaggggg
     6361 caccgaggga tcgatgatca cgttcatgtt caccattccc gccagcatac gaaaaacatc
     6421 ccgaagatcg gtatcacgaa gttccagggt caccggcgtc gtcattgcaa ggggatcacc
     6481 agccgtgggc gtcttgaccg gagaccgggg tatgacttcc tctgccgtat ccggagttgc
     6541 gagatatacc gtcatgacag gggtcggggg atctccgacg gttcttccca gaacaagtct
     6601 tctgctggac cgtaccgtca tgtgcacccc gtcggactcc tgcttcagca caacgctgct
     6661 cacaaggggc gtattaaagg ctttttccca gtaagccgaa ggcagatagg tcccgctcca
     6721 gagaagcacg aggacattgt ctcctgtaga aagaatgtcc ggcgcgggaa gaaggtttcc
     6781 tgtaacctcc atgacggcga gaccggaacc tccctgcatg agggtcactc cctgaatcga
     6841 agggagtgag gaagccagcg tatcggcatt ttcctggcgc gcatccgccc atgaaccgcc
     6901 tggggcgaaa agaatctgtg acaggaagag taaaataaaa gcgacccggg tgatcttcat
     6961 tgccggtcca ccggaatatc gatcttctgt cctgaccatg tgaccaccac cctgtcgttt
     7021 gttatagaga cgaccttccc tctgccgtca ccgaaggaag cacctttttt aagtattacg
     7081 ccatcaccga aaccctcgat atccaccacc gcggcggcat cctgaccttt tatcatcact
     7141 gccttgaccc agatcatggg cgtgcccgca ccttcgaggg ccgtttcagg aagatagtca
     7201 cccgctccgg ggggcatgaa aggcttgcgc cccaccgttg acgcgagtac cgccagctgc
     7261 gtgcttccgg tccttgccgt cacggcgttc ctgaagccct ttgccgtctc gtcaaggcgg
     7321 cgtatctcgt ctcccgtagc cgcgttggcc gcgggaggcg gggtatcact acccctcatc
     7381 gtggagaaca tctggctgaa aacaagggcg gaccagactg tgccggaaac aaggaccagg
     7441 gcaaggatcc attttacaag ccgggggaac cgttctccct cggtgaggtc tctccagagt
     7501 tcctgggcgg aaccaatcca ggagtcagaa gaagtcattc tcatgccccc ccttcggcaa
     7561 ggacagtctc aaggactacc gtccctttga ccattcccgg ttcatccctt gaaagggtta
     7621 ttcctatcat ccttacggcg gttcccattc ttctccagtc ggctatcacg tttagtaccg
     7681 tgtaatatgg ccccacgaaa tcgacctgta ccgcagaatt ccccgcgacc gccttggccg
     7741 gcttcatgct gttgacctga atacctccct tggacagttc cctctctacc gacgaaaaaa
     7801 aagccacctc gtccatcggc agtgttatcc ggtactctcc tatcacgccc agagtggtct
     7861 tgtatgcctg aagaaggcgc gtcttgttct ggatgtccag ttccagtatc ctctgccttg
     7921 tccggagttc ctctatctgc cctctttcac gggtaacaat acggctgaaa acagcctgtc
     7981 cggccagaaa ggccacgaca agaaaaaccg aaccgg
//
LOCUS       DAOTIQ010000070         8011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_10845_length_8011_cov_7.826295, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000070 DAOTIQ010000000
VERSION     DAOTIQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_8011_cov_7.826295"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..65
                     /locus_tag="P5541_08015"
     CDS             <1..65
                     /locus_tag="P5541_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71969.1"
                     /translation="FAVREGGRTVGAGVVTEILD"
     gene            93..242
                     /gene="rpmG"
                     /locus_tag="P5541_08020"
     CDS             93..242
                     /gene="rpmG"
                     /locus_tag="P5541_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRV71970.1"
                     /translation="MADQIGLTCTECKRRNYVTTVNKKKQQKKLEKKKYCKWCKAHTL
                     HKESK"
     gene            264..339
                     /locus_tag="P5541_08025"
     tRNA            264..339
                     /locus_tag="P5541_08025"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Trp,seq:cca)
     gene            370..549
                     /gene="secE"
                     /locus_tag="P5541_08030"
     CDS             370..549
                     /gene="secE"
                     /locus_tag="P5541_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRV71971.1"
                     /translation="MKFFSFVREARAELKRVTWPSRQQVWYSTLVVIAVTFIVAAYLG
                     LVDVLLTAIFSRVIR"
     gene            629..1171
                     /gene="nusG"
                     /locus_tag="P5541_08035"
     CDS             629..1171
                     /gene="nusG"
                     /locus_tag="P5541_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRV71972.1"
                     /translation="MNDKSERHWYIVQTYSGYENRVMANLQQRIATMGMDEKIFHVLV
                     PIEERVTVKDGKSKRTKRKVFPSYVLVDMILDDQSWYVVRHTPGVTGFVGAGSYPIPL
                     TEREVSEIMNKIGKEQIKPKVEIDLKPGDAVRVKAGPFEGQVGPVVEVLEDKGKVKFS
                     VTVFGRDTLVESDYADLEKI"
     gene            1229..1651
                     /gene="rplK"
                     /locus_tag="P5541_08040"
     CDS             1229..1651
                     /gene="rplK"
                     /locus_tag="P5541_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRV71973.1"
                     /translation="MAKKVIAQIKLQLPAGKATPAPPVGPALGQHGVNIMEFCKQFNA
                     KTADQPGMIIPAVLTVYADRSFTFILKTPPASVLLKKAAGLEKASGEPNKVKVGKVTL
                     DQVREIAELKKVDLNANDVNAAMEMIKGTANSMGIEVV"
     gene            1757..2464
                     /gene="rplA"
                     /locus_tag="P5541_08045"
     CDS             1757..2464
                     /gene="rplA"
                     /locus_tag="P5541_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRV71974.1"
                     /translation="MKRRSKRYKAVAEKASAMGLLGLRDAIAFAKENATAKFDESVEL
                     HVRLGVDPRHADQQVRSTIVLPHGTGVVKKVLVIAGGEKFKEAQDAGADFVGGEDMVP
                     KIEGGWLDFDAVIVTPDMMKSVGKLGKILGPRGLMPSAKTGTVTFEVASAIAEIKAGK
                     VEFRVDKFGIIHNSFGKAGFPMENLYENGKALLGAIQKAKPAVAKGQYMKSIAITSTM
                     GASVRVDPAVALKEITE"
     misc_feature    2505..2656
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2667..3200
                     /gene="rplJ"
                     /locus_tag="P5541_08050"
     CDS             2667..3200
                     /gene="rplJ"
                     /locus_tag="P5541_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRV71975.1"
                     /translation="MPSEARIEQVAVLREKLDGAEAVFVCEYRGLTVAKITALRASIR
                     NGGGEMTVAKNTLMEIAMREESLPVPEEMMTGPNSFTITKLDPAAIAKVIRDFAKEKG
                     NEALIIKGAILGNQILKVEQVRALADLPSREVLLAQLAGTLIGPVRGLVTVLSGNTRG
                     LVTCLSRIKEQKEEQAA"
     gene            3304..3726
                     /gene="rplL"
                     /locus_tag="P5541_08055"
     CDS             3304..3726
                     /gene="rplL"
                     /locus_tag="P5541_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRV71976.1"
                     /translation="MEEKKPAKKAASKGLSKDDIIAAIEQMSVLELSELVKELEDKFG
                     VSASAPAMAMPMMGMMPGAAAPAEEEKTEFDVILKSAGSNKIGAIKVVREITALGLKE
                     AKELVDNAPKPVKEGVTKEEAEEIKKKIEEAGAEVELK"
     gene            3881..4510
                     /locus_tag="P5541_08060"
     CDS             3881..4510
                     /locus_tag="P5541_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71977.1"
                     /translation="MSNKDRKGGFAVISVLVILLILTFLSAIIMDLALNAHSTTMEMI
                     ENKKLYNAAQSGAEWGVAMLVANRDDLDDDVKDGISGISFSPTNPVHINAIRATVNGG
                     TYLDSTQPASSMFGTGITVEVDILDCNYEPPTNYHSLLPPVKLPDTGGGGGTPGGLSQ
                     IGSSGYLDPNRNISFGSGGGTMRYFVVRSKAQVSGGREVAVETMVVVTE"
     gene            4507..6915
                     /locus_tag="P5541_08065"
     CDS             4507..6915
                     /locus_tag="P5541_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71978.1"
                     /translation="MNIKKMFFMCIFLIGILPGASLAADQGYIQIFIEPSGVRTDARW
                     SYDGGSTWLESGYKTSKIDVGTYIITFNTVTGWISPSPLSLTVTKNKTSAGTGIYQQG
                     GGGVTVPEDWTVWGQPSYQGENYAPLSFTLINRNILAWVESDRTPTSSVWGSTVKMVD
                     FNVSENIRQLEFPSTQRYLYSTQSNTTMNTLDANSKTVMEDNRFIMPEEEENWLTLED
                     PSETIANQQFTNSTVGGWVGSWITFIKESTKAGLPLSDTGHASPVVTWPDNKWLESLP
                     GYQTYSEGLVPAYETLGGAVIFLPTNDGVIHAFDVDADDDGYFQELWGAMPLSSFLFG
                     VYQEMHKQTGGLVIHPRIPSLGGPILVHDVEDGQGGWLRLLAGSTGEGVGLAVKSASA
                     WDSETGRNVSSSLGNPESVSGHAAGVFALDVTSLTYNQIKQKWSVTNIDLGGGDGFLD
                     LVSSATGNIWTKTAYDEITGGALPSFAGYRTLMMSLARPVTGYTGSNTRTWHMVLLGV
                     DTESKFRLYDINPLTGELYGTTQLSETVPTGTEVELPSKIGAVAPWGETTPRLDEIYF
                     YLSNGSFYSWDLNAGTAPVSLLDVEFKLQGEYYNAEVVQDFDGTFLEVDGETHRFVAF
                     VVRLQKAGQGQGGGIIYGAIVVDVTKLVADGSLPQTLEIGTHWGQTNVFVSESDSVQS
                     MYLAEHMHGNEAFPVSAPFFYDGKLIIAATGYQSTGSERGEYGKVFIADPITGEVQTD
                     TYRDTKLIGGALVDESGVLRVATEDGTILSVDLTDYGLDVPGSGTGGGTSGEVSTVYW
                     KIVN"
     gene            6939..7787
                     /locus_tag="P5541_08070"
     CDS             6939..7787
                     /locus_tag="P5541_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV71979.1"
                     /translation="MRIDTHVHVYPPEVQADWQKIAEREPHFASICSGKVHKWALAED
                     VVARMETDGIDQSWITGFAFRDPGLCRICNDYVKDSVKRYPALLKGLAVVNPMSPGFE
                     KEISRCSEQGTIGVGELFPDGQVFDIADIRQTWRLVAECHSRGLFIMLHTAEPVGREY
                     PGKGRTGPREAAAFCTNHPEVQVVFAHMGGGLWGYETLKEMKLYLQNAWYDTAAAPYL
                     YFPQVFSSVFAAGAGGKLLYGSDFPLLGAERYLKMLDDSGLEEDSISMVMGGNAEDLL
                     KRAKKI"
     gene            7850..7924
                     /locus_tag="P5541_08075"
     tRNA            7850..7924
                     /locus_tag="P5541_08075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7882..7884,aa:Val,seq:tac)
BASE COUNT         2066 a         1940 c         2284 g         1721 t
ORIGIN      
        1 gcttcgcggt tcgcgagggc ggcaggaccg ttggcgccgg cgtcgtgacc gaaatactag
       61 actagtacca ggagaagagg ttcggaaaaa agatggcaga tcagatcgga ctcacatgca
      121 ccgagtgcaa aagacgcaat tatgtaacca cggtcaacaa gaaaaagcag cagaagaagc
      181 tggaaaagaa gaaatactgc aagtggtgca aggcacacac cttgcacaag gaatcgaagt
      241 agtgtagaat acctttcgcg cgcaggtccg tggctcaatt ggcagagcac cggtctccaa
      301 aaccgggggt tgcaggttca agtcctgccg ggcctgccat tatgttcaag ttcaggaggc
      361 cgtgattcta tgaaattttt cagttttgtc cgcgaagcaa gggcggagtt aaagagagtg
      421 acatggccca gccgtcagca ggtctggtac tctactctcg tcgtaatcgc ggtaacattt
      481 atcgtggcgg cctaccttgg actggtcgat gttttgctga cggcgatatt ttcaagggtc
      541 atccgataga gagccaaagg tttttcctat tgggtgaaag ccacttcctc aggaggacag
      601 ggggccgaga aaggccccct tttaagtgat gaacgataag agcgaaaggc actggtacat
      661 tgtccagaca tattcgggtt acgaaaacag ggtaatggcc aaccttcagc agcgcattgc
      721 caccatgggc atggacgaga agatatttca tgttctcgtc ccgatcgagg aaagggtcac
      781 cgtcaaggat ggcaaaagca agcgcacaaa gaggaaggtc ttccccagct atgtccttgt
      841 ggacatgata ctggatgacc agtcctggta tgtcgttcgt cataccccgg gagtcacggg
      901 ttttgtgggg gcgggcagtt accccatccc actgacggaa cgggaagtaa gcgagatcat
      961 gaacaagatc ggcaaggaac agatcaagcc caaagttgag atcgacctca aacccggcga
     1021 cgcggtccgg gtcaaggccg gacctttcga gggacaggtt ggccctgttg tcgaggttct
     1081 ggaagacaaa gggaaggtca agttctctgt aactgttttc ggcagggata cactggtcga
     1141 gtccgattac gccgatcttg agaagatctg aaactgtctt ttaaagccct gcctcgtttc
     1201 agggctaatg taggagggaa actcgcaaat ggcaaagaaa gtaatagcac agatcaagct
     1261 tcagttgcct gcaggcaagg cgacaccagc tccccctgta ggtccggcgc ttggacagca
     1321 cggcgttaat atcatggagt tctgcaaaca gttcaacgcc aaaacggcgg atcagcccgg
     1381 tatgatcatc cccgctgttc tcacagtcta cgcggacagg agttttacct tcattctcaa
     1441 gacgcctccc gcgagcgtcc ttctgaagaa ggccgctggt ctcgaaaagg cctccgggga
     1501 gccgaacaag gtaaaggtgg gaaaagtaac ccttgaccag gttcgggaga tcgcggaact
     1561 caaaaaggtc gacctcaacg ccaacgatgt gaacgccgcc atggagatga tcaagggaac
     1621 cgccaactcc atgggtatcg aggtcgtctg aatcagggag taattttttt cgaaaatgca
     1681 gcatctgccc cactgtgcgg cagaatccgg tgggagagcg cagaagcgtt cgcctggacc
     1741 acggggagga atagaaatga aaaggagaag caaaagatac aaggcagtag cagaaaaagc
     1801 gtccgcaatg gggttgcttg gtctcaggga tgccattgcc ttcgcgaagg agaacgccac
     1861 cgcaaaattt gacgaaagcg ttgaattgca cgttcgcctt ggggttgatc ccaggcatgc
     1921 cgaccagcag gtccgcagca ctattgttct ccctcacggg accggtgtgg tcaagaaggt
     1981 tcttgtcata gccgggggcg aaaagttcaa ggaagcccag gatgcagggg ccgattttgt
     2041 tggtggagaa gacatggttc cgaagatcga gggaggatgg cttgatttcg atgccgtcat
     2101 agttacccct gatatgatga aatccgtcgg taaactcggg aaaattctcg ggcctcgtgg
     2161 tctcatgcca agcgccaaga cgggcaccgt aacctttgaa gtggcttccg ccattgccga
     2221 gataaaggcc ggcaaggtgg aattccgtgt cgacaagttt ggtatcatcc acaactcctt
     2281 cggcaaggca ggcttcccga tggagaacct ctacgagaac ggcaaggctc ttctcggggc
     2341 catccagaag gcaaagccgg ctgtggccaa ggggcagtac atgaagagta ttgcgattac
     2401 atccacaatg ggagcatctg taagggtgga tcccgctgtt gcattgaaag agatcaccga
     2461 atagcccgtt aggtgtttta cctgggctga gcagatttaa aaaaacagaa tactggtaac
     2521 ggccgaagac agctggtgcg ctaagaggcg cttaaatttt tgcccgccga ggtctggaag
     2581 aagcgaaacg caaggttttg tcctgtgtat cggtctcccg gatcccggag gggttcggga
     2641 gattttttat gtaaggaggt gaataaatgc cttcagaagc aagaatcgag caggtagcgg
     2701 tattaaggga gaaactggac ggggctgaag cggtcttcgt atgcgaatac aggggcctca
     2761 ctgttgcaaa gatcacggct ctaagggcat cgatccgcaa cggcggcgga gagatgaccg
     2821 tagccaagaa taccctcatg gaaattgcca tgcgcgaaga atcattgccg gtccccgaag
     2881 agatgatgac tggccccaac tcgtttacga tcacaaagtt ggaccccgct gccattgcaa
     2941 aggtgattcg cgattttgca aaggaaaaag gaaacgaagc gctgattata aaaggcgcga
     3001 tcctgggtaa ccagatactg aaagtggagc aggtcagggc actggccgac cttccgtcaa
     3061 gggaagtcct gcttgcgcag cttgccggta ctctgatagg acctgtaaga ggccttgtaa
     3121 cggttctttc agggaatacc cgcgggctcg taacatgcct gtcccggata aaggaacaga
     3181 aagaagagca ggcggcatag tctgtccggg aagaacaaaa accaaaacaa cgaaaatcat
     3241 aaggaggaac atgaaatgac cgcagccaaa aaggcagcag aagttgaaga gggagcagaa
     3301 gtgatggagg aaaagaagcc cgcgaagaag gcggcttcca agggactttc aaaagatgac
     3361 atcattgccg ctatcgagca gatgtccgtt ctcgaacttt ccgaactggt caaggaactc
     3421 gaggataaat ttggggtatc agcctcggct cccgcaatgg cgatgccgat gatgggcatg
     3481 atgcccggcg ccgcagcacc cgccgaggaa gagaagaccg agtttgacgt tatcctcaag
     3541 agtgccggtt ccaacaagat aggcgccatc aaggtcgttc gcgagatcac cgccctcggc
     3601 ctcaaggaag ccaaagaact cgtcgacaac gcccccaagc cggtcaagga aggcgttacc
     3661 aaggaagagg ccgaagagat caagaagaag atcgaagagg ccggcgccga ggtcgaactc
     3721 aagtagtttt ttgaacaaac cgcaagtcga tttacgatgt atctcaagag gcccccgcaa
     3781 gggggctttt ctgttatcat tttgaaaata tgctcttcct tcatgcctga tgcggtataa
     3841 tgctactgta aagaagaagt gcgagtctgg ggggccgagg gtgtcgaaca aggatcgcaa
     3901 gggcggtttc gcggtgatat cggtgctggt gatactcctg atactgactt tcctcagcgc
     3961 catcatcatg gatcttgccc tgaacgctca cagcacgacc atggagatga tcgagaacaa
     4021 gaaactctac aatgccgccc agagcggggc cgagtgggga gtagccatgc ttgtggcaaa
     4081 cagggacgac ctggacgatg atgtaaagga cggtatctcg gggatatcct tcagtccaac
     4141 caaccctgtc cacattaacg ctattcgcgc gaccgtcaac ggaggaacat acctggactc
     4201 cacccagccc gcttcgtcca tgttcgggac aggtatcacc gtggaggtcg acatcctgga
     4261 ctgcaactac gagcccccca ccaactacca ctcgctcctg ccccccgtta aactgccaga
     4321 tacaggaggg ggaggaggaa caccaggagg tctttcccag atcggttcct cgggctacct
     4381 tgatcccaac aggaatatat cttttgggag cggaggggga acaatgcgct actttgtcgt
     4441 gagatcaaaa gcgcaagttt ccggcgggag ggaagttgct gtggaaacaa tggttgtggt
     4501 aacggaatga atattaaaaa aatgtttttt atgtgtattt ttctgatagg gatcctcccg
     4561 ggagcctcgc tggctgcgga tcagggttac atccagatat tcatagaacc ttcgggagtg
     4621 cgtaccgatg caaggtggtc atatgacggt ggttcaacgt ggctggagtc tgggtacaag
     4681 acatcaaaaa ttgatgtcgg tacttatata ataactttta acactgtcac aggatggata
     4741 tctccgtctc cgcttagtct gactgtgacc aagaataaaa cctctgccgg taccggtatc
     4801 taccagcagg gtggaggagg tgtcacggtt ccagaggact ggactgtgtg gggccagcca
     4861 tcctaccagg gcgaaaacta tgcccccctt tcttttaccc tcataaacag aaatatactg
     4921 gcatgggtcg aatccgacag aacgccgacc agttcggttt ggggttcaac ggtcaagatg
     4981 gtggatttta atgttagtga aaatattagg cagttagaat ttccatcaac ccagagatac
     5041 ctttatagta cccagtccaa cacaacgatg aacacccttg atgccaacag caagaccgtg
     5101 atggaagaca accgcttcat tatgcccgaa gaggaagaaa actggcttac gcttgaggat
     5161 ccatctgaaa caatagccaa tcagcagttc acaaactcaa cagttggcgg ttgggtcggc
     5221 agctggatca cattcataaa ggaatcaacc aaggcgggtc ttccactttc ggataccggg
     5281 cacgcgagtc ctgtggtcac atggccggac aacaaatggc ttgaatccct tcccgggtac
     5341 cagacctact ccgagggact tgtaccagcc tatgagacgt tggggggagc tgtaattttt
     5401 cttcccacca atgatggcgt aatccacgct tttgatgttg atgccgacga tgatggttat
     5461 ttccaggaac tctggggcgc gatgcccctt tcatccttcc tttttggtgt ataccaggag
     5521 atgcacaagc agacaggggg ccttgttatt caccccagga taccatcact tggtgggccc
     5581 atactggtgc acgatgtgga ggatggccag ggtgggtggc tgcgccttct ggccggctct
     5641 acgggtgagg gtgtgggcct tgcggttaaa tccgcaagcg catgggacag cgaaacaggt
     5701 cgtaatgtca gttccagcct tggtaacccg gaaagcgtaa gcggccatgc cgcgggagtg
     5761 tttgcccttg atgtgacctc tctgacctat aaccagatca agcagaagtg gtctgttacc
     5821 aatattgacc tgggtggtgg tgatggtttc ctggacctgg tttcatccgc aacaggcaac
     5881 atttggacaa agacagccta cgatgaaata acaggtggtg cccttccttc ctttgcgggt
     5941 tacaggaccc ttatgatgtc gctggcgagg cctgtcacag gctacacagg gtcaaacacc
     6001 aggacctggc acatggttct gcttggagtt gatacggaaa gcaaattccg tctctacgac
     6061 atcaaccctc tcaccggtga actttatggc acaactcagc tttcggaaac ggtgccgaca
     6121 ggcacagagg ttgaactccc ctcaaagata ggggccgttg caccctgggg agagacaaca
     6181 ccgaggctcg acgagatata cttctatcta tccaacggtt ctttttacag ttgggattta
     6241 aacgcgggaa cagctccggt atcactgctg gatgtggaat tcaaacttca gggcgaatat
     6301 tacaacgcag aagttgtcca ggattttgac ggaacattcc ttgaggttga tggagaaact
     6361 caccgttttg ttgcttttgt tgtccgcctc cagaaggcgg gacagggtca gggtggcggg
     6421 attatctacg gagccattgt ggttgatgtt acaaaacttg ttgcagacgg atcccttcca
     6481 cagaccctgg aaataggtac ccactggggt cagaccaacg tgtttgtgtc cgaaagtgat
     6541 tccgtgcaaa gtatgtacct tgcggagcat atgcatggca acgaggcatt ccctgtctcc
     6601 gccccgttct tctatgacgg caagctcatc attgccgcta cgggatatca atctactggt
     6661 tcggaaagag gggaatacgg caaggttttt attgccgatc ccattaccgg tgaggtccag
     6721 accgacacct accgggacac aaaactcatt gggggggcac tggttgacga aagcggtgtt
     6781 ctcagggttg cgacagaaga tgggactatc ctgagtgttg atctcaccga ttacggtctg
     6841 gatgtccctg gaagcggaac gggaggcgga acctcaggag aggtcagcac cgtctactgg
     6901 aagatcgtca attgatcagg gagcgctata atagacacat gcgaatagat acccacgtcc
     6961 acgtgtaccc ccctgaagta caggccgact ggcagaagat cgccgaaagg gagccccact
     7021 ttgcctccat atgctccggc aaggtccaca agtgggcact tgcggaggat gtcgttgccc
     7081 gcatggaaac agacgggata gaccagtcat ggataacggg atttgccttc cgagaccctg
     7141 ggctttgccg gatctgcaac gattacgtca aagactctgt gaagagatac ccggcgttgc
     7201 tgaaaggcct cgctgtagtt aaccccatgt cccctggttt tgaaaaggaa atatccagat
     7261 gctcggaaca gggaacgata ggagtgggtg aactcttccc cgacggtcag gttttcgaca
     7321 tcgcggatat acgccagacc tggcgcctgg ttgccgaatg ccacagcagg ggtcttttta
     7381 tcatgcttca tacggcagag cccgtagggc gtgaatatcc cggcaaaggc agaacagggc
     7441 cacgtgaagc agctgctttc tgtaccaacc atccggaggt gcaggttgtt tttgcccaca
     7501 tgggtggagg cctctggggc tacgagactc tcaaggaaat gaaactctat ctccagaacg
     7561 cgtggtacga tacagccgct gccccctacc tttattttcc acaggtgttt tcctctgtat
     7621 tcgcggctgg agcgggggga aaactgcttt acggtagtga ttttcctctc ctcggtgcgg
     7681 agagatacct gaagatgctg gatgattccg gactcgaaga agactccatt tcgatggtca
     7741 tgggcggtaa tgccgaagat ctgctcaaaa gggcaaaaaa gatttgagaa tgtgttgaca
     7801 tacgtcgttg aggtcgatat actaaccttc gcgccgacct ggcgtaagag ggcgtatagc
     7861 tcagcggaag agcacctgcc ttacacgcag ggggtcacag gttcgaaccc tgtttcgccc
     7921 accaggaaac caatcaaagg ttataataga tgcaatgggg ttgtagctca gtcggttaga
     7981 gcgccggcct gtcacgccgg aggtcgcgag t
//
LOCUS       DAOTIQ010000071         7674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_11446_length_7674_cov_8.229426, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000071 DAOTIQ010000000
VERSION     DAOTIQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11446_length_7674_cov_8.229426"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..921
                     /locus_tag="P5541_08080"
     CDS             598..921
                     /locus_tag="P5541_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71980.1"
                     /translation="MKTIETGGIVLGTFMIFFMLALMGACWWAGISYDAIYRGVRDCR
                     EIGSEIQLLQAENPADPEKTRKLAEMEKQWLSCARMTNNRMDKTISGRLVARIFNFSS
                     IEIPE"
     gene            953..2746
                     /locus_tag="P5541_08085"
     CDS             953..2746
                     /locus_tag="P5541_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRV71981.1"
                     /translation="MFGKLLHVDLGTGSFREHNVPEDIAIKYIGGKGLGIYLLDSLME
                     PGTGPLDPGNVLMFLTGPFTGTAFPTSGRFVVVTKSPLTGMYVDSHSGGHFAKEMRLA
                     GYDGIIVTGKASGPVYIWLEDGKAEIRDAGEIWGKTVDDTVHSVREMTHAEARVAVIG
                     PGGENLVPIATITMDSDSDKFRAGVAGRGGTGAVMGSKNLKAIAVRGSGAIKLKDEKS
                     FREQTAEIIKGVNASDSIHTRRIVGTSSLVESMSRTGILPTRNFQQGYFVPIYGLVST
                     NLRHYTKRDVACSNCTIMCGKVLEDEGHAVKVEYESIALLGSNDGIGKMPQLAKAVRV
                     CNQLGIDSISAGGAVAFAMECRQRGILPDAPEFGDSEGQIRLLEDIAYRRNLGATLSK
                     GVREAAKEIGNNTEHFAIHVKGMELPGYEPRATWGMALAYATADRGGCHQRAWTVLGE
                     LDGLIPRFKTDGMALTVKSAQDERAAAYSLVVCDFVPYAQEMALACLKHVTGWDISET
                     EYLAAGERIWNHIRVFNMREAGISRKDDTLPPRMFEDPLPMPPRGEETVSLSRKDFDT
                     MLDEYYSLRGWDSDGRPTAATLERLGINQRI"
     gene            2766..3797
                     /locus_tag="P5541_08090"
     CDS             2766..3797
                     /locus_tag="P5541_08090"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRV71982.1"
                     /translation="MWKIGMAGFGNVGQGLARILEKKQDSLMEKYGFEYKVTFIADPV
                     KGSLLNPAGIDLKSLLDEMDGKGDLKGHPDATAMDTIQLISGKHADVITEATYTDLKT
                     GEPGVTHIRTALENGIHVVSTNKGPVSIALPELMELAAGNGVSYFFEGVVMSGTPTMN
                     LAREALAGCDINGAMGIVNGTTNYILTMMEEGMSYEEALKKAQELGYAEADPTGDVEG
                     WDAAVKVQVMAKVLMGKPVQVDKVDRTGITGITPADIESASARGTRIKLIAEVGMDGG
                     KLVAKVAPREIPLTHPLAGIMGATNAITLTTDHLGEVTIVGPGAGRVETGQALLTDLL
                     AVHRIHQKA"
     gene            complement(3794..4612)
                     /locus_tag="P5541_08095"
     CDS             complement(3794..4612)
                     /locus_tag="P5541_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV71983.1"
                     /translation="MRVTMLDVAAAAGVNKATVSRVLRGDPRISSSTSEKVWSTIKKL
                     GYRPDAIARGLSSRSSDTVGLFFRDLAVPWAGEFMAGFERVTSRQGCECIFRSTGSDR
                     QQIRNAMFRLISRRVDGLVWIDCLPEPDSEVPGLAVGLEYAAGVSVVVDHDHAISSLM
                     ALADGRAVEIQQGEKPLFPGIVNLLPRQKAGKSGTVRVFDGVSPPCCEPLPDDVIVVC
                     TTRKSPVPAKGIWKLYWPAFELGTLSARVLLNTIKGRGVRPDTVRLLPVLSGPA"
     gene            complement(4808..6448)
                     /locus_tag="P5541_08100"
     CDS             complement(4808..6448)
                     /locus_tag="P5541_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRV71984.1"
                     /translation="MPITVEIAGGGVHSFDPPVTGQEILNSIGAGMDRKIVAWHVNGY
                     LRPIDWPVHNDSSVDFVDTSSFEGMAAYRSTLTFVIALACRGAYGEGLSVRHSISDGY
                     FCELGSGKVSPEQIRLVKEAASFIISKDLPITREIHDLDRARRIFEDQNEPDTANLLK
                     CAGIDPVILNVCDGVYGFFHGPLAPSTGYISSWDLEPFNDGMVVRFPTVTYPRELPPF
                     QPAKKLAGVFREYGEWLHILGVQTMENLHARVSGGEAKQLVLVSEALHSQKLNRMSEM
                     ILSDRKIRLVCISGPSGSGKTTTADRLSIHLRAMGLQPVALYLDDYFVNRENTPRDEE
                     GNYDFESIDALDLGLINEHLSILLDGGSVNVPRFNFLTGSREKGPELHMERGDILVIE
                     GIHGLNERLTGSIPEEFKFSIYISPLTGITLDRHNRTSSTDNRLLRRLVRDHRLRGKS
                     AETTLAQWPSVIRGAHRYIFPYQEHADEMFNSALVYELSVLKGYAEPLLKTVREESPE
                     FGEAQRLLNISRYIPYIPADFVPGNSILREFIGGGCFE"
     gene            complement(6454..7437)
                     /locus_tag="P5541_08105"
     CDS             complement(6454..7437)
                     /locus_tag="P5541_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV71985.1"
                     /translation="MILKKACLLVLAILFSVSSLAFAAETNLSKDNLFVSAEWLKANQ
                     GNVLIIDARPSDMYKKGHIPGAVNAEWTYFANMKGSPGSPGWGDLFPKDTLAKKIGAL
                     GVSGKKPVVVYGDGGGWGTEGWVTWILRLSGVTGAKKLDGGFNAWVAAGGKPTTAPTK
                     PKAVAFSIPAFQGGYNVTTPWLASKLGEVTIVDARTPFEYEGGRLFQEKRGGHIPGAI
                     NIPFDIVFKDDRTVREIEDLQAIFSMKGLDPEQEIIVYDTAGVRSAHLVLVLRMAGYG
                     KARNYDSSFQEWAGNMELQVVQGPEPGSVEKEEVAVEDPQPLADQPQQPTT"
BASE COUNT         1904 a         2059 c         2080 g         1631 t
ORIGIN      
        1 aggatacgcc gcttttcttc ctattgccat acaccagaaa tgagcttaac tcccctctgc
       61 cccctatcgt gaaaaacacg gggccgaaag ctcatcgctt tcggccccgg cctgcccgtt
      121 cattcccata cgcctcacag agacttttta cccctagtgg ttcacggcaa gagaagaaaa
      181 aagataaaca ctgctttctg tgcccagccc gaacgaactt tcaaacggaa tcttttcccc
      241 tgacactgcc cctttaagat tatcaatagc aacgttttcc tctgccaaaa ccgaattgtc
      301 cgccatatac cagttccctg cgctgtcctt ttgccacata tcgattcccc cgatcaggtt
      361 tgtcgtgctg ctggatattg aaaagtcatc gaaaaccgaa ggacagccac atcttattcg
      421 tagcaccctg aaaaacctcc cgaaagatcg atctctcctg cctgttgctt ccagcaagta
      481 taatgttctt accgaaattg tcaactccag tttatttcta taagaaaaat aaaccaactt
      541 ctcagacaag tacaattatg actcaggccg gtttaaggat aaaatgtgca taccagtatg
      601 aaaactatag agaccggagg gattgttctg ggtactttca tgatcttttt catgcttgcc
      661 ctgatgggtg cgtgctggtg ggcgggaatt tcctacgatg ccatttaccg cggggtcaga
      721 gattgccggg agattggatc tgagatccaa ctattgcaag ccgaaaaccc tgccgatccc
      781 gaaaagaccc gaaaactggc cgagatggaa aaacaatggc tttcctgcgc gaggatgaca
      841 aacaaccgga tggacaaaac catttcagga aggcttgtcg ccagaatatt caacttcagc
      901 agtatagaaa tacccgaata atgaacaacc ttaagtcaag ggagggaaag caatgttcgg
      961 gaaattgctt catgtagatc ttgggaccgg ctctttcagg gaacataatg tccccgaaga
     1021 catcgcgata aaatacatag gcgggaaggg cctggggatc tatcttctgg attcgcttat
     1081 ggaaccggga acaggccccc tggacccggg taatgtcctg atgtttttga cggggccttt
     1141 caccgggacc gcattcccga cttcgggacg ttttgtcgtc gtgacaaagt ccccgctgac
     1201 aggcatgtat gtcgacagtc attccggcgg tcacttcgcc aaagagatgc gtttggccgg
     1261 gtatgacggc atcatagtca cgggaaaggc ttccgggccg gtctatatct ggctggagga
     1321 cgggaaggcg gagatccgtg acgcgggaga gatctggggt aaaaccgtgg acgacacggt
     1381 ccactccgtg agggagatga cccatgccga agcccgggta gccgttatcg gccccggtgg
     1441 agagaacctt gtaccgatag ccaccataac catggacagc gacagcgaca agttcagggc
     1501 gggagtcgcc ggcaggggcg gcacaggcgc tgtaatggga agcaaaaacc tcaaggccat
     1561 tgcggtcagg ggaagcggag caataaaact gaaggacgaa aaatcgttca gggagcagac
     1621 agccgagata attaaaggcg tcaacgcaag tgactcgatc cacaccagga ggatcgtcgg
     1681 aacctcctca ctggtggaat ccatgagcag gacggggatc cttccgacaa gaaattttca
     1741 gcaggggtat tttgtgccga tctacggact tgtttcaaca aacctccgac actatacaaa
     1801 aagagatgtc gcctgctcga actgtaccat aatgtgtggc aaagtccttg aggatgaggg
     1861 tcatgcggta aaggtcgaat acgaatccat agcccttctc ggaagcaatg acgggatcgg
     1921 gaaaatgccg cagctcgcca aagcggtaag agtatgcaac cagctaggca tcgactctat
     1981 ttcggccgga ggcgccgtcg cgttcgcgat ggaatgccgg cagaggggga tccttcctga
     2041 cgctcccgag ttcggtgatt ccgaaggaca gatccgtctt ctcgaggaca tagcctaccg
     2101 aaggaacctc ggagccaccc tttcgaaggg ggtccgggaa gcagcaaaag agatcgggaa
     2161 taacacggaa cactttgcca ttcacgtcaa gggaatggaa cttcccgggt atgaaccgag
     2221 agccacatgg ggaatggccc tcgcgtacgc tacggccgac cgtggaggat gccaccagag
     2281 ggcatggacc gtcctggggg aactggacgg gcttattcca cgcttcaaaa ccgatgggat
     2341 ggcactcacc gtcaagtctg cccaggacga acgagcggca gcctattcgc tggtcgtatg
     2401 cgattttgtt ccctacgccc aggaaatggc ccttgcctgt ctgaagcatg ttacgggatg
     2461 ggatatcagc gaaactgaat acctcgcggc aggagagcga atatggaacc acatcagggt
     2521 cttcaacatg agggaggccg gtatatccag aaaagatgac actctcccgc caagaatgtt
     2581 cgaggacccg ctcccgatgc caccaagagg agaagagacg gtatcccttt cccgaaagga
     2641 tttcgataca atgcttgacg aatattattc gctgcgcgga tgggactccg atggacgccc
     2701 cacggctgcc actctcgaga gacttggcat aaaccagcgt atctgaccgg aggtgctttg
     2761 aagacatgtg gaagatagga atggcgggtt ttggaaacgt aggccaggga cttgcgagga
     2821 tacttgagaa aaaacaggat tccctgatgg agaaatacgg ttttgagtac aaggtgacct
     2881 tcatagccga cccagttaag ggttcccttc tgaacccggc ggggatagac ctcaagtcgt
     2941 tgctggacga gatggacgga aagggtgacc tgaaaggaca tcccgacgcg acggccatgg
     3001 ataccattca gctgatctcc ggcaaacacg ccgacgttat taccgaggca acatatacgg
     3061 acctgaagac gggagagccc ggcgtcaccc atatcaggac cgccctggaa aacggcatac
     3121 acgttgtgtc gacaaacaag ggacccgttt caatagccct gccggaactt atggagctgg
     3181 ccgccgggaa tggagtctcc tatttctttg agggcgtggt catgagcgga actccaacca
     3241 tgaacctggc cagagaggcc cttgcggggt gtgacatcaa cggcgccatg ggcatagtta
     3301 acggaaccac caactacatc ctgaccatga tggaagaagg catgagctac gaagaagcac
     3361 tgaagaaagc ccaggaactt ggatatgccg aggcagaccc aacgggggac gttgaggggt
     3421 gggacgcggc agtcaaggta caggtaatgg caaaagtgct tatgggcaag cctgttcagg
     3481 tcgacaaagt ggaccgtaca ggaatcactg gcataactcc cgcggatata gaatccgcat
     3541 cagcgagagg gacccggatc aaactgatcg ccgaggttgg catggacgga gggaaacttg
     3601 tagcgaaggt agctcccagg gaaatcccgc tgacccatcc ccttgcgggc atcatgggtg
     3661 ctacaaacgc cataacgctg acaacagacc atctgggtga ggttaccata gtcggacccg
     3721 gggctggaag ggtagagacg ggccaggccc tccttacgga cctgcttgca gtccacagga
     3781 tccaccaaaa agcttaggcg gggccggaaa ggactggcag aagccttact gtatccggcc
     3841 tgacgcctct tccttttatt gtattaagca ggaccctggc agaaagggtc ccgagttcaa
     3901 aggccggcca atagagtttc cagatgccct tggcaggaac aggacttttc cgggtggtgc
     3961 agacaacgat gacatcatca ggaagaggtt cgcagcaggg aggagatacg ccgtcgaaaa
     4021 ccctcacggt accgctcttc cctgcctttt gccttggaag aaggttcact atcccgggaa
     4081 acaacggctt ttctccctgt tggatctcca cagctctccc atctgccagg gccatcaggc
     4141 ttgaaatagc atgatcgtga tccactacca cggagacacc cgccgcgtat tcgaggccta
     4201 cggccaagcc gggaacttca gaatcgggct ccgggaggca gtctatccac accaggccat
     4261 caacccttct ggagatcagg cggaacattg cgttgcgtat ctgttgcctg tccgacccgg
     4321 tcgaccggaa aatgcattca cagccctgcc tcgatgtcac acgctcgaat ccggccataa
     4381 actctcctgc ccacggaaca gcaagatccc tgaagaataa ccccacagtg tcgctgctgc
     4441 ggcttgaaag tccccttgcg atagcatccg gcctgtatcc cagcttcttg atggtgctcc
     4501 agaccttctc ggacgtcgag gaactgatcc tgggatctcc cctgagaacg cggctgaccg
     4561 ttgccttgtt gacccccgca gcagcagcga catccaacat ggtcacgcgc atggcacaaa
     4621 acctgaagga aggagaaagg cgtcaggagg aaggaggcag gaaaaactgt gaatcgcaaa
     4681 ggaagaaatg cttaaaaaaa gcttacgcca aagatcgccc ggacaaagta ctctccgtgg
     4741 tacagaaccc gggttttgat ccccctgccg cctgtcgcct gtcgcctgct tcctggctcc
     4801 ttcatcctta ttcaaaacac ccgcccccga taaattcgcg gaggatcgag ttcccgggga
     4861 caaaatcagc cggtatgtag ggtatgtatc tggaaatatt cagaagcctc tgggcctctc
     4921 cgaactccgg ggactcctcc ctgacggttt tcagaagcgg ttcggcatat cctttgagta
     4981 cagaaagttc gtaaaccaga gcagaattga acatctcgtc ggcgtgttcc tggtaaggga
     5041 aaatgtagcg atgggcccct ctaatgacag agggccactg tgcaagggtg gtttcggcac
     5101 ttttgccccg aagcctgtgg tcccggacaa gccttctgag caacctgttg tccgtcgaac
     5161 ttgtccggtt atggcggtcc agagtgatac cggtcagggg ggaaatataa atgctgaact
     5221 tgaattcttc ggggatggag ccagtaagcc tttcattcag tccgtggata ccctcaataa
     5281 caaggatgtc tcccctctcc atgtggagct cggggccctt ttccctgctt cccgtcagaa
     5341 aattgaacct tgggacatta acacttccgc catcaaggag aattgacaga tgttcgttta
     5401 tcaggcccag gtcaagggca tcgatagatt cgaaatcgta attgccctct tcatccctgg
     5461 gagtgttctc tctgttgaca aaataatcgt ccaggtacag ggcaacgggc tgtaaaccca
     5521 tggccctcag gtggatagaa agtctgtcgg ctgtggtcgt cttgcccgat cccgaaggtc
     5581 cggaaataca gacgagcctg atctttctgt ccgaaaggat catctcggac atgcggttaa
     5641 gtttctggct atgaagagcc tcgctcacga gaaccagctg tttggcctct ccgccggaaa
     5701 cccgcgcgtg gagattctcc atggtctgca ccccgaggat atggagccac tcaccgtact
     5761 ccctgaaaac ccccgccagt ttcttcgcgg gctgaaacgg gggaagttcc ctgggatagg
     5821 ttaccgtggg aaaacggacc accattccat cgttgaaggg ctccaggtcc caggatgaaa
     5881 tataacccgt agatggagcc agagggccgt ggaaaaaacc gtagactccg tcgcacacgt
     5941 tcagtattac tgggtcgatc ccggcacatt tcaggaggtt ggcggtatca ggctcgtttt
     6001 gatcttcaaa tatccttcga gccctgtcca gatcgtggat ctccctcgtg atcgggaggt
     6061 ccttcgatat aatgaaactg gctgcctctt tcacaagcct gatctgctcg ggtgacacct
     6121 tccctgatcc cagttcgcag aaatatccgt cgctgatgga atgcctgacc gaaagccctt
     6181 ccccataggc gccccggcac gccagggcga taacaaaggt tagggtgctc cggtaagccg
     6241 ccatgccctc aaatgaggac gtgtcaacaa agtcgacgga ggaatcattg tgaactggcc
     6301 agtcgatagg acggagatac ccgttaacgt gccaggcaac tatcttgcga tccattcccg
     6361 ctccgatact gtttagtatt tcctggcccg taacaggggg gtcgaaagag tggacccccc
     6421 caccggctat ctcaacggta ataggcatcg atcctatgtg gttggctgct gaggctggtc
     6481 cgccaaaggt tgcgggtcct cgacagctac ctcttccttt tcgactgatc cgggctccgg
     6541 gccctgaacg acctgcaact ccatgttacc tgcccactcc tggaaggatg aatcgtagtt
     6601 gcgggccttt ccgtatcccg ccatccgaag caccagcacc aggtgggccg accttacgcc
     6661 ggcagtatcg tagactatta tctcctgctc cgggtcaagc cccttcatag aaaaaatggc
     6721 ctggaggtct tctatctcac ggacagtcct gtcatcctta aaaactatgt caaagggtat
     6781 gttgatagct cctggaatat gtcctccccg tttttcctgg aagagcctgc ctccctcgta
     6841 ttcgaaagga gttcgggcat caactatggt cacttcaccc agcttggagg ccagccaggg
     6901 tgtagtcaca ttgtagccgc cctgaaaggc tggtatcgaa aaagcgactg ccttgggttt
     6961 tgttggagcg gtggtgggtt ttccccctgc agcgacccag gcgttgaatc caccgtcaag
     7021 tttttttgcg cccgttactc cggaaagccg caggatccag gttacccagc cctcggttcc
     7081 ccagccgccc ccatcgccat agaccacgac aggtttcttt ccgctgacac cgagtgcgcc
     7141 tatcttcttg gcaagggtat ctttcgggaa cagatctccc cacccggggg agccggggct
     7201 gcccttcatg ttggcgaaat aggtccattc cgcgttcacc gctccgggga tgtgtccttt
     7261 cttgtacatg tctgacggac gagcatcgat tatcagaaca ttcccctggt ttgccttgag
     7321 ccattccgcg ctgacaaaaa gattgtcctt tgaaagattt gtctcagcgg caaacgcaag
     7381 tgaggacaca gagaacagaa tagcaaggac tagcaggcat gcctttttca agatcatcat
     7441 cctttctcgt ggattgctgc ggacccggaa aacaaaagat cctgggaaca ttttatacgt
     7501 ttgaagcggg ggggtcgatg gggaattacg taaaaaaaca aatgcgtggg acgtgaatcg
     7561 tgaatcggag aagcaaggcg actggaaaca gaagactggc ggcaggaagg acaaaaaatc
     7621 tgtcattctg agcgtagcga agaatctggg tctagaacca gatccttcga tcgc
//
LOCUS       DAOTIQ010000072         7665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_11458_length_7665_cov_8.346518, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000072 DAOTIQ010000000
VERSION     DAOTIQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_7665_cov_8.346518"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1140
                     /locus_tag="P5541_08110"
     CDS             145..1140
                     /locus_tag="P5541_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRV71986.1"
                     /translation="MSGRPNIAVICPPDEETDHLHLYEKILGDIADIHFLSGAQPQER
                     EEVLAKSDVVVSASCAPSEISPSEIALLKPGGLLQLLYSGADNVPFDLVPENVMIASN
                     AGVFAVPIAEHVLAMVLCLLKRIVPRYEELKKGVFDRKQQNRILRGKTCGILGFGGNG
                     RAIARIMRSAGVKVIAINRSGKTGEPVDEIWDNSRLDDLLRLSDVLVLTTPLTESTRG
                     LIGDRELSLMPSDAILVNVARGAVVDQGALYSHLVSHPGFCAAIDTWWSEPDAHGQFR
                     LEFPFFGLPNFLGSPHVADNVPGTMSLAVTSALENVRGYLEGGKLKGLLDRTDYQ"
     gene            complement(1172..1681)
                     /locus_tag="P5541_08115"
     CDS             complement(1172..1681)
                     /locus_tag="P5541_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71987.1"
                     /translation="MDPLLFLGSFFRRKKLPLTHEDIIKRASSLDDYFKRLQGKRILV
                     FDPPFWGFHDLFIDGKGRVLLVCLKAEGESFAFAGDERGASVMQKFGPGPELNAEEPL
                     EPGILEWILYDDYIVYRGPFFPISRHPYYLGKVAATFPYDGTIDKSTIPGKISELQEW
                     YKAEKEKRP"
     gene            complement(1686..2240)
                     /locus_tag="P5541_08120"
     CDS             complement(1686..2240)
                     /locus_tag="P5541_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71988.1"
                     /translation="MIRKGMMALAAYSLEPEIQKGSHPEDSFRTGFLNEVLEILSRLQ
                     QEEKIDEFFLLPDFGFDLGVFIGKEEQTRSIFFNLKMYMGAKPRVVEIGDQNGSGPEI
                     ELLQLNTARSALAAGSFRWILVDITKPRGNRRYSIFTTDQAKEGLMGGLNKKKQNSIK
                     LASVMTFPMTWDELSGKLASFLAE"
     gene            2432..3940
                     /locus_tag="P5541_08125"
     CDS             2432..3940
                     /locus_tag="P5541_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRV71989.1"
                     /translation="MLFSKTFIPTARDAQKNITDPSVARLSRAGYIAADQETRTLRLL
                     PLGFLLWERVISDALDLAREEGIQVAGFEDVPEDAMTVSKKLVKSYRQLPLTFFSGDM
                     KRWIASGLAPEAGSAGDMRDRFLEMAGTLCRHAGLDPRSGETIEDGRKYISLASSKGA
                     WFAKEGFVCSSCSWCGDDASPAGPVERPVGEALPIKEVETPGADTIAELCSQLGVEPS
                     ATMKTMFYAAEQGGTKEVVAVLARGDHQINDRKLSHVLGGSVVRFADPLEIREAVGDL
                     AGYLGPVGLPGGIKVLADRYVEGSQGLVAGANKPGFHLLNVCWGRDFSPSVVADLVSI
                     QEDLPCPACGSRIVPSHLARVAVAGRESGPSELSFQGEGGTACNAYRWEGTLCITPLV
                     LALAGEDRIPGRYAPFDVNVLIASMQNDDAVGLGKIMAERLEEKGLRVLLDERNERAG
                     VKFSDAELLGIPVTIVAGREASEGVVEAWLPDGSRGEMPADSIMEQVLRWVR"
     gene            4045..4410
                     /locus_tag="P5541_08130"
     CDS             4045..4410
                     /locus_tag="P5541_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71990.1"
                     /translation="MTKKVILLALVVVMMASTAALAFGGGMKDFGPRMDFDGSGRTRM
                     APRQGLQRDRDVQKIEYPQEIQAQFGELRRTHLEMRLALAQEEPDIDKAKLLFEKAQG
                     IRNEISKWRFERYIESLKK"
     gene            4667..6553
                     /gene="dnaK"
                     /locus_tag="P5541_08135"
     CDS             4667..6553
                     /gene="dnaK"
                     /locus_tag="P5541_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV71991.1"
                     /translation="MSRTVGIDLGTTNSVVAVLEGGQPSVIPNAEGSRTTPSVVGFTK
                     DGERLVGILARRQAVLNPRGTVSSIKRFMGRNHDEVAEEMKIVPYTVVKGSNDSVRVE
                     VEGKQYAPEEISAQVLRKLAQDASAWLGEKVTDAVITVPAYFNDSQRQATKNAGKIAG
                     LNVLRVINEPTAAALAYGMDKKNNETVMVFDLGGGTFDVSILDVGEGLCEVRSTAGDT
                     HLGGDDFDKCVVDWLADEFKKQEGIDLRNDRQALQRLYEAAEKAKCELSSRSSTEISL
                     PFISADANGPKHLSFNLTKAKFEELTRHLVERCLEPMKQALKDARLEAKDINEIIMVG
                     GSTRIPAVQELVKRFTGGKNLNMTVNPDEVVAVGAAVQAGIIKGEVKDVLLLDVTPLS
                     LGIETLGGVMTKIIERNTTIPARRSEVFSTAEDNQPAVDVVVLQGEREMARDNRKLGN
                     FKLEGIRPAPRGLPQIEVTFDIDANGIMNVSARDKETGKEQKITISNSTNLDESEINR
                     MVEDARVHAEEDKKHRAWADARNELESASYQVERMINELGENAPAHEKARGEDLIREA
                     QEAISKEEDISRLQSLTQDLKQAAAALSQHSAPGSGDTGEPSPGETDKKEGEDVIDAE
                     FNEG"
     gene            6626..7579
                     /locus_tag="P5541_08140"
     CDS             6626..7579
                     /locus_tag="P5541_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRV71992.1"
                     /translation="MKFRDYYEILGVPRNASQSDIQKAFRKLARKFHPDVNKDPEADK
                     RFKEINEAYEVLRDPDKRKKYDALGANWKNGQDFSPPPGWEGVFQFGGDGGGGSSFSD
                     FFEAIFGSMGGFSTMDGRHFGGRPLRRRGRDLESKIELTLDEIAAGGKKHVEFEVLER
                     GNDGRARRTRRSLDVIIPKGVIQGSRIRLAGQGEKGSGGAPPGDLYLEVQVRKDPCFE
                     IEGQDLRITVDITPWDAALGGSVEVPVLGGSVTMKIPPGTQSGQVLRIRGKGLPGKGD
                     CVGSILVEVRIVIPKQLSPREKELFEALKEESKFKPEAKKR"
BASE COUNT         1828 a         1926 c         2303 g         1608 t
ORIGIN      
        1 tacacttttt acaaaaaggg ttttccagga ctttccggtt tttatcctgt cttctttggg
       61 aaatcccgag caatctacag agagggtaaa caggatttgt caaaagtgta cggggtgaca
      121 cctgttaagg aggaggaatt cgttttgagt ggacgtccga atattgccgt gatctgcccc
      181 cccgacgagg agacggatca tctgcacctg tatgaaaaaa ttcttgggga catcgctgac
      241 atccattttc tgagcggcgc tcaaccgcag gaacgcgaag aagtgcttgc caaatcggat
      301 gttgtcgtct cagccagttg cgcgccttcg gagatctcac catcggagat agctttgctg
      361 aagcccgggg gattgcttca gcttctttat tccggggctg acaacgttcc gttcgatctg
      421 gtcccggaga atgtaatgat cgcctccaac gcaggggttt ttgccgtacc tattgccgag
      481 cacgttcttg cgatggtgct ttgtctgttg aaaaggatcg tcccgagata cgaggaacta
      541 aagaaaggag tctttgaccg aaagcaacag aacaggattc tcagggggaa gacctgcggg
      601 atccttggat tcggaggcaa cggcagggcg atagcgagaa taatgagatc tgcaggagta
      661 aaggtcattg caataaacag gagtggcaag accggagagc cagtcgacga aatatgggat
      721 aacagcagat tagatgacct cttgcgttta tccgatgttc ttgtgcttac gacccccttg
      781 acagagtcta cgaggggtct cataggggat agggaactgt ctctcatgcc ttcggacgcg
      841 atcctggtga atgtggcaag gggagcagtg gtggatcagg gggcacttta cagtcacctt
      901 gtttcccatc cgggtttttg cgcggccatt gatacctggt ggtcagagcc ggatgcccac
      961 ggtcaattca ggcttgagtt ccctttcttt ggattgccta actttctggg ttctcctcac
     1021 gttgctgaca atgtcccggg gacgatgtcc ctggctgtca cgtcggcact cgagaatgtc
     1081 aggggatatc tcgaaggcgg aaaactgaag gggctgcttg acaggaccga ttaccaatag
     1141 tgtcgcaagg ccggcttaat ctgttccccg ttcacggcct tttctccttt tctgccttgt
     1201 accattcctg aagttcggag atcttcccgg gaatagtgct tttgtcgatc gtaccgtcgt
     1261 aagggaaagt agccgcgact ttcccaaggt agtaggggtg ccggctgata ggaaagaacg
     1321 gtcccctgta gacgatatag tcgtcataaa ggatccattc caggataccg ggttcaaggg
     1381 gttcttcggc attcagttcg gggccggggc cgaacttctg catcacggac gccccgcgtt
     1441 cgtcaccggc gaaagcaaat gattccccct cggccttgag gcagaccagg aggaccctcc
     1501 cttttccatc gatgaacaga tcgtggaagc cccagaaagg aggatcgaag accaggatcc
     1561 ttttgccctg gaggcgcttg aaatagtcat caagggaaga tgcccttttg ataatgtctt
     1621 cgtgggtcag tggaagcttt ttcctccgga aaaaactgcc cagaaaaagg agcgggtcca
     1681 tgaggtcatt cggccaggaa cgatgccagt ttgcccgaca gttcatccca ggtcatgggg
     1741 aaggtcatta ccgaggccag cttgatgctg ttctgcttct tcttgttcag gcctcccatc
     1801 aggccttcct tggcctggtc ggttgtgaaa atgctgtacc tgcggttacc ccggggtttg
     1861 gttatatcca ccagtatcca acggaaggat cccgccgcca gcgcactcct ggcggtattc
     1921 aactgcagga gctcgatctc gggccctgat ccgttctggt cgcctatctc aaccaccctc
     1981 ggtttcgccc ccatatacat cttgagatta aagaagatcg acctggtctg ctcctctttg
     2041 ccaatgaaaa cgcccaggtc gaacccgaaa tcgggcagaa ggaagaactc atctatcttc
     2101 tcctcctgct gcagccttga tagtatctcc agaacctcgt tcaggaatcc ggtacggaag
     2161 ctgtcttcgg ggtgtgatcc cttctgaatt tcgggctcaa ggctgtatgc cgccaaagcc
     2221 atcatcccct tccttatcaa aagcgtactc ccccggttca tccattcggg ggagctgagt
     2281 ttacccgact gcggtccata cgtatccgat tataacccaa caatgttaga taatgaaaaa
     2341 actggccttc ctggcggcat ggcctatact tatgttacag atggatatgc gtggatctgg
     2401 aaaatcattt gaccgcagga ggtacattgt catgcttttt tcaaagacct tcatacccac
     2461 agccagggat gcacagaaga acatcactga cccttcggta gctcgtcttt caagggcggg
     2521 ttacattgca gctgaccagg agacgagaac gctgaggctg ctgcccctcg gctttctgct
     2581 gtgggagcgc gtcatatcag atgcccttga ccttgcaagg gaggaaggaa tccaggtcgc
     2641 gggtttcgag gatgtaccgg aagatgccat gaccgtttca aaaaagcttg tgaaaagcta
     2701 caggcagctc ccccttactt ttttctctgg ggatatgaaa cgatggatag cctcaggctt
     2761 agcccccgag gcaggttcgg caggggatat gagggacagg ttcctcgaaa tggcaggcac
     2821 cctttgccgc catgccggtc ttgacccccg ctcgggggag acgatcgagg acgggaggaa
     2881 gtatatctct cttgcaagct caaaaggagc ctggtttgca aaagaaggtt tcgtatgttc
     2941 ctcctgcagc tggtgcggtg atgatgcctc tcctgcaggt ccggtcgaga gacccgttgg
     3001 ggaggcgtta ccgataaaag aggtcgagac gcccggggcg gacaccattg cagaactctg
     3061 tagccagctg ggggtagagc cttccgccac aatgaagacc atgttctacg ccgccgaaca
     3121 gggcggcaca aaggaagtgg tagcggtcct ggcaaggggt gaccaccaga taaacgacag
     3181 gaagctttcc cacgtcctcg gcggatcggt ggtcaggttc gccgaccccc tggagatcag
     3241 ggaggctgtg ggagaccttg cgggctacct cggtcccgtt ggtctgcccg gggggatcaa
     3301 ggtcctcgcc gacaggtacg tggagggaag ccaggggctc gtggcagggg ccaacaaacc
     3361 cgggtttcac cttctgaacg tctgctgggg gcgtgatttt tcaccctccg tagttgctga
     3421 tcttgtaagc attcaggaag atctaccgtg cccggcctgc ggatccagga tcgtgccctc
     3481 tcacctggcc cgtgtcgccg tcgctggcag agagtcaggg ccctctgagc tgtcctttca
     3541 gggggagggc gggaccgcat gtaacgccta ccgctgggag ggtaccctgt gcataactcc
     3601 cctggtgctg gctcttgcag gagaggacag gatacctgga cgttacgccc cgttcgatgt
     3661 gaacgttctt atagcctcca tgcagaacga tgacgccgtg ggtctcggca agattatggc
     3721 tgaaaggctc gaagagaaag gattgcgtgt cctccttgat gagaggaacg aaagggccgg
     3781 tgtgaaattt tccgatgccg agcttttggg cattcccgtc actatagtgg ctggcaggga
     3841 ggcttcagag ggtgtcgttg aagcctggct tcccgacgga agccgcgggg aaatgccggc
     3901 ggactcgatc atggaacagg ttttgcgctg ggttaggtag atacccctaa gggagaatcg
     3961 gtctttttac ccatcaccgg tcgtacgtcc cggaatagaa tgacactgca agggagtttc
     4021 gaactaaatc cggaggtggg taatgtgacg aaaaaagtaa ttcttctcgc tcttgttgta
     4081 gttatgatgg ccagtacagc ggcccttgcc tttggcggcg ggatgaagga ttttggtccc
     4141 aggatggact ttgacggctc ggggcgtaca aggatggccc cacgtcaggg gcttcagagg
     4201 gacagggatg ttcagaagat agaatatccg caggagatcc aggcgcagtt cggtgaactc
     4261 aggaggaccc atcttgagat gaggctcgct cttgcccagg aggaacccga tatcgacaag
     4321 gcgaagcttc tttttgagaa agcccagggc ataaggaacg agatatcgaa atggcgtttt
     4381 gagagataca ttgaatccct gaagaaatag atctttctcc cttcccttgc gcggggccga
     4441 aattggcccc gcgcttttta tgtcctttca acgaaatata aagaaataaa gcctgaaaca
     4501 gaaataaagg cagaaaacag ggaataatat cccttcaaac cccaagacca aaagtcaaaa
     4561 tattgcctga ttaatattga cctatataat tgtcatattg aaggtcaaca ttagtcaggc
     4621 ttttccacgg gctattcccg ggatgagcca acaggaggga tgcgatatgt ctcgaactgt
     4681 cgggatagat cttggaacga ccaactcggt ggttgctgtg cttgaggggg ggcagccatc
     4741 ggtaattccc aatgccgagg ggagcaggac caccccttcg gtggtgggat tcaccaaaga
     4801 cggtgagcgc ctggttggga tcctcgccag aagacaggcc gtgctcaacc ccagggggac
     4861 tgtctcctcc atcaagcgtt tcatgggaag gaaccacgac gaggtcgccg aggagatgaa
     4921 gatagtcccc tacactgtcg ttaaggggag caacgactcc gtgcgcgtag aggtggaggg
     4981 caagcagtac gcgccagagg agatcagcgc ccaggtcctg agaaagctgg cacaggatgc
     5041 ctcggcatgg ctgggagaga aggtgaccga tgcggtcatc accgttccgg catactttaa
     5101 cgattcccag cgccaggcaa cgaagaatgc gggaaagatc gctggcctta acgtgctgag
     5161 ggtcatcaac gaaccgacgg cggccgcact agcctacggt atggacaaga agaacaatga
     5221 gaccgtcatg gtcttcgacc tgggaggggg gaccttcgac gtctctatcc tggatgtcgg
     5281 agaaggtctc tgcgaggtgc gctccacagc cggtgatact catctcggag gggatgactt
     5341 cgacaagtgc gttgtagact ggcttgccga tgagttcaag aagcaggagg gaattgacct
     5401 caggaacgac aggcaggctc ttcagaggct ctacgaggcg gcagagaagg ccaagtgcga
     5461 gctctcgagc cgttccagca ccgagatcag cctgcccttc atctcggccg atgccaacgg
     5521 tcccaagcat ctgtccttca acctgacgaa agcgaagttc gaggagctca ccaggcatct
     5581 ggtcgaacga tgccttgaac ccatgaagca ggccctgaag gatgccaggc tcgaggccaa
     5641 ggatatcaat gagataatta tggtaggggg ctccacgagg atcccggccg tacaggagct
     5701 ggtaaagagg tttactggcg gcaagaacct taacatgacc gtgaaccccg acgaagtggt
     5761 ggccgtaggg gcagcagtcc aggcagggat catcaaggga gaggtaaagg atgttctgct
     5821 tctggacgta acccctctat cgctgggtat tgagaccctc ggcggggtca tgacgaagat
     5881 tatcgaaagg aacaccacca tacccgctcg cagatcggaa gtattctcga cagcagagga
     5941 taaccagcct gcggtggacg tagtggtact ccagggcgag cgcgaaatgg cccgggacaa
     6001 cagaaagctg ggcaacttca aactcgaggg gatccgcccc gcccccaggg gactgcccca
     6061 gattgaagtg acctttgaca tcgacgccaa cggtatcatg aacgtttccg ccagggacaa
     6121 ggagacggga aaggaacaga agataaccat aagcaactcg acgaaccttg acgagagcga
     6181 gatcaaccgc atggtcgagg atgcacgggt ccatgccgag gaagacaaga aacatcgtgc
     6241 ctgggcagat gcccgcaacg aactagaatc tgcttcatat caggtagagc ggatgatcaa
     6301 cgaacttggc gaaaacgcgc cggcccatga aaaagccagg ggcgaggatc tgatcaggga
     6361 ggctcaggag gccatatcga aggaagagga catctcaagg ctccagtcgc tgacgcagga
     6421 ccttaaacag gctgccgcgg cgctctcaca gcactccgca cctggttcag gtgataccgg
     6481 ggagccctct ccaggggaaa ccgataaaaa agaaggcgaa gatgtgatcg acgccgagtt
     6541 caacgagggt tgatgcggat agaatagtgg catgccggag gcggtgcctt aataaacagc
     6601 aagctgtcag ggtggagggg tttcaatgaa gtttcgagac tactacgaaa tacttggtgt
     6661 tccgaggaac gcatcgcaaa gtgatataca gaaagctttc aggaaactgg caaggaagtt
     6721 ccaccctgac gtaaacaagg atccagaggc ggacaagcgt ttcaaggaga tcaacgaggc
     6781 ctacgaggtc ctccgcgatc ccgacaagag gaagaaatac gatgctctcg gggccaactg
     6841 gaagaacgga caggacttct ccccccctcc cggatgggag ggggtattcc agttcggcgg
     6901 cgatggagga gggggcagct ccttcagcga cttctttgag gccatattcg gctccatggg
     6961 cggattcagc accatggacg ggcggcattt tggtggtcgc cctctccgcc gaaggggtcg
     7021 ggacctggag tcgaagatcg agctcaccct cgacgaaata gccgccggcg ggaagaagca
     7081 tgtggagttc gaggtcctgg agaggggcaa cgacggaaga gcccggagga cccgaagatc
     7141 ccttgatgtc atcattccga aaggggtgat ccagggatcg cggatccggc tggccggtca
     7201 gggggagaaa gggagcggcg gtgctccccc gggggacctc tatcttgagg tccaggtcag
     7261 aaaggatccc tgtttcgaga tcgaggggca ggatctccgg atcacagttg acattacgcc
     7321 ctgggacgcg gccctggggg ggagcgtaga ggtccctgtc cttggcggtt cggtgacgat
     7381 gaagatacct cccgggacgc agagtggcca ggtgctaagg ataaggggca agggactgcc
     7441 aggcaagggt gactgcgtag gcagcatact tgtcgaggtc aggatcgtga tcccgaagca
     7501 gctctccccg cgggagaagg aactttttga ggctttgaag gaagagtcga aattcaagcc
     7561 cgaggcaaaa aaacgttaaa ctggcagata ggtgttcaaa taggtgtcag acctacacgg
     7621 gctgttgccc aaatagttta tatcaaggcg caagtggctg aatac
//
LOCUS       DAOTIQ010000073         7641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_11496_length_7641_cov_6.589902, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000073 DAOTIQ010000000
VERSION     DAOTIQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11496_length_7641_cov_6.589902"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5541_08145"
     CDS             <1..704
                     /locus_tag="P5541_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71993.1"
                     /translation="EAGLNIALLALVTVVLLVFPLVVGAEVRLFVDKNTVRVHDPELG
                     QPSILNNFQSNGHRTHNYLALVWDSNSVWVYDIRTHQWLNIQGFSAVSGLLSDDLAMA
                     WERERVAVFSAQDRSWVLSDTTPSPITGQQISRKMAAVTCEDAFVVYDPVLKTWQTAG
                     DFEVKSAQTGDNLAVAWDDDDVVIYDLTMHQWIVKQGIAPQACIIEPFKVSIYSSDRI
                     HVYDAMSHRWQDMPR"
     gene            745..1935
                     /locus_tag="P5541_08150"
     CDS             745..1935
                     /locus_tag="P5541_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV71994.1"
                     /translation="MGTDRTMTTAGKNFLKRILAVMMVVLVLSASSAGASGLTVAVDT
                     FLSQGSSLFLGSSISELLRTELIGNRDITVVERGQLGAVAEQQRLSLSGVVETGTAVQ
                     VGKLVGARYFILGAVSKFGTLLVLTARLVDVETGNVMKSFEQISHDGESGVTLATRNL
                     GAEMLAFFSGDSPAEGDPMNDYRYYLYEALGYYNLGQYSRSIPFWEKMTKLSPRNETL
                     RFILGGVYYQAGRYNDALLSAQQSVTWDEKLAEAHLLVGKSYFMIGDFHRATPPLDRA
                     LELDPGLTEALFLKGQAFKNRKRLDEAVDLLVAAIHSDESYIPAYLALGQLLLDVGAY
                     DDAAGVLIPAAKIEPGNPNIRFLLGMAHVLRGDVKGADEQLRLLEGIDNGLASKLREL
                     MDSQ"
     gene            2187..2714
                     /locus_tag="P5541_08155"
     CDS             2187..2714
                     /locus_tag="P5541_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV71995.1"
                     /translation="MVAHNVTDSCSCAYMVASSYLHQGEYEKGKEIMMKVIDGCYDSI
                     VNYWGDAEKLRTIAASELAMYAGGEGDHEVIDWIEDRVKADAVTDRVAVRDAKGILQG
                     RTKLRDVVRFHKARAYKNADMAEKAKEVLNELSFASGKVFVDGEVHGLDDAIKKLQED
                     IAAVASAILKFFKAA"
     gene            2746..3228
                     /locus_tag="P5541_08160"
     CDS             2746..3228
                     /locus_tag="P5541_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRV71996.1"
                     /translation="MKKMAVMIVAGLVVFAFAGTALAEMTIAVTDFRSLGCQFYLGGA
                     VAEQVRNHLSEEDGWIVVEKGQIAAVAAEQRLNLSGLVDDDTAVKVGNYVGARYVIVG
                     SVNGMGDTYTLSARLVDVETGIGLIGFETSTGEGEEGVLDASRVLAEQIVIELGGSTE
                     "
     gene            3316..3942
                     /locus_tag="P5541_08165"
     CDS             3316..3942
                     /locus_tag="P5541_08165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRV71997.1"
                     /translation="MEIRRCPWSESHSMLTSYHDMEWGVPVQDDRKMFEFLLLESFQA
                     GLSWLTVLKKRDNFREAFMGFDPEKVSAFGEGDIQRLLLDRGIIRNRQKISAAISNAR
                     AFLALREKEEKFASWLWGFVDGEPLRNSWKSENEIPAETELSKKISKELRSRGFSFVG
                     PVVVYSHMQATGLVNDHLVDCFRYEELSPCNSRSSSKERSSTPAMDSM"
     gene            complement(3864..4220)
                     /locus_tag="P5541_08170"
     CDS             complement(3864..4220)
                     /locus_tag="P5541_08170"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRV71998.1"
                     /translation="MALVSLKNFTKFANEITESLPRALFGHLNGGIAIQPSVKEDGDY
                     LIMGEYIEDPSMGNMILLYYGSFLEMLGNADIPEWKNEIEETIIHELRHHMESMAGVD
                     DLSFEEDLELQGDSSS"
     gene            4356..5567
                     /locus_tag="P5541_08175"
     CDS             4356..5567
                     /locus_tag="P5541_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRV71999.1"
                     /translation="MKFSKRVTGMQSSPIRKLVPYAEAAKAEGKKIYHLNIGQPDIKT
                     PIGFLDSIKKFAEDVIAYSTSHGSYELIDAMIGYYKGYNMNFERKNIIVTNGGSEALS
                     FAFMAICDPEDEILVPEPFYANYNAFGSSVSVKMLPITTKAEKGFHLPPKEEILKKIT
                     SRTKGILLSNPGNPTGVIYTSEEMEMIADIAMEKELFIIADEVYREFVYDGLKYTSFG
                     NIVRVEDRVIIVDSISKRYSACGARIGCIISKNDELMAQVMKLAQGRLCVPTLEQVGA
                     VELYKTPASYLEEVNKEYQSRRDILYSKLREMPGVICDEPKGAFYVVAKLPVDDAEKF
                     VIWMLQEFHVNKETVMMAPAEGFYATPGLGKDEVRLAYVLKKEDLAKAMDILKAGLEA
                     YPGRIKTASNF"
     gene            5699..6073
                     /locus_tag="P5541_08180"
     CDS             5699..6073
                     /locus_tag="P5541_08180"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV72000.1"
                     /translation="MEKVLVCVKALADIQRIKIAYALSKGELCLCSLQEFLGLSPSTV
                     SRHVSIMKQAGMVESRTDGKWRYFRLIPDIEKTPCGDLLGWLIKNIENDPEIVAQAEQ
                     VSLMLEQQPEKCSPCNAGRRSC"
     gene            6074..7264
                     /gene="arsB"
                     /locus_tag="P5541_08185"
     CDS             6074..7264
                     /gene="arsB"
                     /locus_tag="P5541_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRV72001.1"
                     /translation="MVKQFNNGERKMTNVFERYLTVWVALCIAGGIVLGKLAPGFAKA
                     LDGMAIMVNGAPVVSIPIAICLFFMMYPIMVKIDFREVVKAGKSGKPVFLTLFVNWAI
                     KPFTMYAIAMFFLGFLFKGFIGPDAVDLVKMPFGLNLPVGSVHGSGIVVLENGIKMLQ
                     VPLWRSYLAGAILLGIAPCTAMVLVWGYLSKGNDSLTLVMVAINSLTMLVLYGVLGGF
                     LLGVGKLPVPWQALVLSIAIYVALPLIAGYLSRKWIIAAKGVTWFTEKFLHILSPITI
                     VALLTTLVLLFSFKGEVIVANPLTILWIAIPLFIQTILIFAIGYWLARKIKLPYADAA
                     PAAMIGASNHFEVAIATATMLFGLSSGAALATVVGVLIEVPVMLMLVKFCLKTRHWFP
                     DQVS"
BASE COUNT         1870 a         1801 c         2126 g         1844 t
ORIGIN      
        1 cggaagcggg tctgaatatt gctttgctgg cgctagtgac tgtcgtgctg ttggtttttc
       61 cccttgtagt cggcgccgaa gtaaggctct ttgttgacaa gaacactgtc agggtccatg
      121 atcccgagct tggccagccg tcgatcctga acaacttcca gtccaacggg cacaggacgc
      181 ataattacct ggctctggta tgggactcga actccgtctg ggtctatgac ataaggaccc
      241 accagtggct gaacatccag ggtttttccg ctgtttccgg cttgctgtca gatgacctgg
      301 caatggcctg ggagagggaa agggtcgctg tgttcagcgc ccaggacaga agctgggtcc
      361 tgagcgatac gactccttct cctatcacgg gacagcagat atccagaaag atggctgccg
      421 ttacctgcga ggatgccttt gtggtttacg atccggtgct gaagacatgg cagacagcag
      481 gcgatttcga ggtaaagagc gcccagaccg gcgacaatct cgctgtggca tgggatgacg
      541 atgatgtggt catatatgac ctgacgatgc atcagtggat agttaaacag ggcatcgctc
      601 cccaggcatg catcatagag cctttcaagg tttccattta ttcttcggac aggatccatg
      661 tttacgacgc catgtcccat cgctggcagg atatgcctcg ctagaaagag atgaccggta
      721 aaatctagga ccaagggggc gaaaatggga acagacagga ccatgacaac agcgggaaaa
      781 aattttttaa agcggatatt ggccgtgatg atggtggtgc tggttttgtc tgcttcttcc
      841 gcgggagcgt cggggctgac cgttgctgtc gataccttcc tttcgcaggg gtcaagcctt
      901 ttccttggtt catccataag cgaactgttg cgaacggaac tgataggcaa cagggatatt
      961 actgtcgtgg agagagggca gttgggagcc gtggccgaac agcagaggct ctccctttcg
     1021 ggagtagtag agacagggac cgccgtccag gtaggcaaac tcgtcggggc caggtacttt
     1081 atccttggag ctgtgagcaa gttcgggaca cttctggtcc tcacagcaag acttgttgat
     1141 gtcgagaccg gaaacgtgat gaaaagtttc gaacagatat cacatgatgg ggagtcaggg
     1201 gtaactctgg cgaccaggaa cctgggtgcc gaaatgcttg cctttttctc aggtgactcg
     1261 cctgccgagg gtgaccccat gaacgactac cgctactatc tttacgaggc cctcggttac
     1321 tacaacctcg gtcaatattc caggtcgatc cccttctggg aaaagatgac caagctgagt
     1381 cccaggaacg aaaccctgcg cttcattctc ggaggtgtct attaccaggc tggaagatat
     1441 aatgacgccc tcctttccgc gcaacagtcc gtaacatggg atgaaaaact ggcagaggcg
     1501 cacctgctgg tcgggaagtc atacttcatg ataggtgatt tccacagggc aacaccgcct
     1561 ctggacaggg cgctggaact tgaccctggg ctgaccgagg ccctttttct gaagggtcag
     1621 gctttcaaga accggaaaag acttgatgaa gctgtggatc ttctggttgc agctatccat
     1681 tccgacgaat cctacatacc tgcctatctt gccctggggc agttgctgct ggatgtaggg
     1741 gcctacgatg atgcggctgg tgttctgatc ccggcggcga agatcgagcc gggaaacccc
     1801 aatatcaggt ttcttctcgg gatggcccat gtgcttcggg gagacgttaa gggcgcggac
     1861 gaacagctga ggctgctgga aggtattgat aatggacttg cctcaaaact cagggaactt
     1921 atggattcac aataaagtta ttaaaaaagt gttttcaaag gagagtgtgg aagatgagat
     1981 caagacgttc ggtgaaatat cttttggctg ttatggcggc ggtatttgcc cttcttgtcg
     2041 cctccgcatc ggtctttggc tgaggcggcg gtggtggggg tggcacctcc actgaagaga
     2101 aaaacgcgaa ggaaaccgaa aaatacgagc agatcttcat caagaacggt catctcacgg
     2161 gcaaggacct gagcgacgcc gagaaaatgg tggcgcataa cgttaccgac agttgctcct
     2221 gtgcctacat ggtcgcgtca agttatctcc accagggtga gtacgaaaag ggcaaggaaa
     2281 taatgatgaa ggttatcgac ggatgctacg actccatcgt caactactgg ggggatgcgg
     2341 aaaaactccg tactatagct gcgtcggaac tcgccatgta cgcagggggc gagggtgacc
     2401 acgaggtcat tgactggatc gaggatcggg tcaaggctga tgctgtaacc gacagggtag
     2461 ccgtgaggga tgccaaggga atacttcagg gtcgcaccaa actcagggat gtggtccggt
     2521 tccacaaggc cagggcctac aagaatgccg acatggctga aaaggcaaaa gaggtcctga
     2581 atgaactttc ctttgcttcg ggcaaggtgt tcgtcgatgg tgaagtccat ggccttgacg
     2641 acgctatcaa aaagctgcag gaagacatcg cggcggttgc atcggctatc ctgaaatttt
     2701 ttaaagcggc ctagattttc ctttttcggg aaacggaggt ggctcatgaa aaaaatggca
     2761 gtaatgatag tggcggggct tgtggttttt gccttcgcgg ggacggcctt ggccgaaatg
     2821 accatagcag ttactgactt ccgttccctt ggctgccagt tctaccttgg tggtgccgtc
     2881 gcggaacagg tccggaacca tctttcagag gaagatggct ggatcgtggt agaaaaaggc
     2941 cagatagccg ctgtggctgc cgaacagagg ttgaacctgt ccgggcttgt tgatgacgac
     3001 accgcggtca aggtcggcaa ttatgttgga gcgaggtacg ttatagtggg ctccgtcaac
     3061 gggatggggg atacttatac tctctctgca aggttggtcg atgtggagac cggaataggt
     3121 ttgatcggtt tcgagacatc aactggagaa ggtgaggaag gagttctgga tgcatccagg
     3181 gtcctggccg agcagatagt gatcgaactg ggaggctcta ccgaataaaa atccttgcaa
     3241 ttttgcgctg cacagaatgc ttttggcggg ggcaatctgt tgtccccgcc atttttgact
     3301 ggagatgtaa acaagatgga aatcaggcgc tgcccctggt ccgaatctca ctccatgttg
     3361 acaagttatc acgacatgga atggggagtt cccgtccagg acgacaggaa aatgttcgaa
     3421 tttctgttgc ttgaatcttt tcaggccggt ttgagctggc ttacagtcct taaaaagagg
     3481 gataatttca gggaagcctt catgggattt gacccggaaa aagtttcggc ttttggtgaa
     3541 ggggatatcc agaggcttct tctggacagg gggatcattc gcaacaggca gaagatctcc
     3601 gcggccatat ctaacgccag ggcctttttg gccctgcgcg agaaagagga gaaatttgcc
     3661 tcctggttat ggggtttcgt cgatggggaa cccttgagaa actcatggaa gagcgaaaat
     3721 gagatcccgg ctgagacaga actgtcaaag aagatcagca aagaactcag gtcgagaggt
     3781 ttcagctttg ttggacccgt agtcgtatat tcccacatgc aggccacggg gttggtaaac
     3841 gaccacctgg tcgactgttt ccgctatgaa gagctgtcgc cttgcaattc caggtcctct
     3901 tcaaaggaga gatcgtccac tcctgccatg gattccatgt gatgtctcag ttcatgtatt
     3961 atggtctcct cgatctcgtt tttccactcc ggaatatcgg cgtttcccag catttccaga
     4021 aatgacccgt aatacagcag gatcatatta cccatggaag ggtcttcgat gtattcaccc
     4081 attatcaggt aatctccgtc ctctttgaca gacggctgaa tcgctatgcc cccgttcaga
     4141 tgtccgaaga gagcccgggg aagagattcg gttatttcat ttgcgaattt cgtgaaattt
     4201 ttcagactga ccagagccat ggttttatct ccctccataa gggtcgggta tataatgtgc
     4261 agtattcagt ttggtgcgaa agttgtccaa atattccaga tttatgctag aattatgttg
     4321 ctgcaatcac aaacggacag ggaggtagag aattaatgaa attctcgaaa cgggtaacgg
     4381 gaatgcaatc gtctccgatc cgcaagcttg taccatacgc cgaggccgcc aaggccgagg
     4441 gcaaaaagat ctaccatctc aacattggcc agcccgacat caaaaccccg atcggttttc
     4501 tggattctat caaaaaattc gccgaagatg ttatagcata ttccacttct cacggcagtt
     4561 atgaacttat cgatgcgatg atcggttact acaagggtta taacatgaac ttcgaaagaa
     4621 agaacatcat agtaaccaac ggcggaagcg aggcgctctc tttcgctttc atggccattt
     4681 gtgatcctga agacgagatt ctggttccgg agccattcta cgcaaactac aacgcctttg
     4741 gctcctcggt aagcgtaaaa atgcttccca taaccaccaa agccgaaaag ggtttccatc
     4801 tcccgcccaa agaagagatc ctcaaaaaaa taacctccag aaccaaaggg atactgcttt
     4861 cgaacccggg aaacccgacc ggtgtcattt acaccagtga agagatggaa atgatcgccg
     4921 acatagcgat ggagaaggaa ctcttcataa tagccgacga agtttacagg gaatttgtct
     4981 atgacggcct gaaatatacc agttttggca atatcgtcag ggttgaggac agggtcatta
     5041 tcgtggactc tatctccaag cgatacagtg catgcggagc caggatcggc tgcatcatat
     5101 ccaaaaacga tgaacttatg gcccaggtga tgaaactggc ccagggcagg ctctgtgttc
     5161 cgacccttga gcaggtcgga gctgtggagc tttacaaaac ccctgcttcc tatctggaag
     5221 aggtcaacaa ggagtatcag agccgcaggg acattctcta ttccaaactc agggagatgc
     5281 cgggagttat ctgcgatgag ccgaagggtg ccttctacgt ggtagcaaaa cttcccgtcg
     5341 atgatgcaga gaaattcgtc atctggatgc tacaggagtt ccacgtgaac aaggagacag
     5401 tcatgatggc tccggcggaa ggtttttatg ccacaccggg acttggcaag gacgaagtca
     5461 ggctcgccta cgtcctcaaa aaggaagatc ttgcaaaggc gatggacatt ctcaaggctg
     5521 gtctcgaagc ataccccgga aggatcaaga cagcttccaa tttctagatt tttattaaaa
     5581 gccgaaacat cggacaccaa aagccgtccc ttgcaggggg cggctttttt cttgaccagg
     5641 agtgaagtat ctgatatcat ttcgtcgttt ggccaaatga gcaagaggag agtgggttat
     5701 ggaaaaggtt ctggtctgcg tcaaggccct tgccgacatc cagagaatta aaatcgcata
     5761 cgccctttca aagggagagc tatgcctgtg cagccttcag gaatttcttg gcctgtcccc
     5821 gtcgaccgtt tccaggcatg tttccatcat gaaacaggcc ggaatggtgg agtcgcgtac
     5881 cgatggcaag tggcgttact tcaggctgat tccggatatt gaaaaaactc cttgcggaga
     5941 tctgctcgga tggttgatca aaaacatcga gaatgacccc gaaatcgtgg cgcaggcaga
     6001 acaggtatct ctaatgctgg aacagcaacc agaaaagtgt tctccatgca atgcaggaag
     6061 gaggtcttgt tgaatggtga agcaattcaa caacggcgaa cgcaagatga ccaatgtttt
     6121 cgaacgttac ctcactgtat gggtcgccct ttgtatcgcg ggtggcatag tactgggaaa
     6181 gttggctccc ggtttcgcca aagcccttga cggcatggcc atcatggtca acggggcgcc
     6241 ggtagtgtca attcctatcg ctatatgtct attcttcatg atgtacccca tcatggtgaa
     6301 gatagatttc agggaagttg taaaggcagg gaaaagcggc aagccggtct ttttgaccct
     6361 tttcgtcaac tgggccataa agccttttac catgtacgcc atcgccatgt tcttcctcgg
     6421 attcctgttc aagggtttca tagggcccga tgcggttgac cttgtcaaga tgcctttcgg
     6481 tttgaacctg ccggtcggtt ctgttcacgg atcggggatc gtagtccttg agaacggtat
     6541 caaaatgctt caggttccgc tctggaggag ctaccttgcc ggagcaattc ttctggggat
     6601 cgctccctgc actgccatgg tcctggtatg gggttacctc tcgaaaggga acgatagcct
     6661 cacccttgtc atggtggcga tcaactccct tacgatgctt gtcctctacg gcgttcttgg
     6721 cggcttcctg ctcggggtag gcaagctgcc ggtaccatgg caggccctgg ttctttctat
     6781 cgccatatac gttgccctgc cgctgatcgc aggctatctc tccaggaagt ggataatagc
     6841 cgcgaaaggg gttacatggt tcaccgagaa gttcctccat atcctttccc cgataacgat
     6901 cgttgccctt cttacaacac tggtgcttct tttcagtttc aagggcgagg tcatagtcgc
     6961 aaacccgttg acgatcctgt ggatagccat tccgctcttc atacagacca ttctgatatt
     7021 tgccataggc tactggttag cacgtaaaat aaaacttccc tacgcggatg cagctcctgc
     7081 ggccatgata ggcgcttcga accacttcga ggttgcaatt gctacggcga ccatgctctt
     7141 tggcctttcc agcggcgcgg cccttgcgac tgttgtaggc gttctgatag aggttccggt
     7201 gatgctgatg ctcgtaaagt tctgtctgaa aacacgacac tggttcccgg atcaggtttc
     7261 ctgaacagga ttttatttat gccgaatact tcaggaatga cgaatgacga atgcctgatg
     7321 ccgaacatat tgaattgcaa cgggtaaggg gggattggga tgagagataa ggtccttatc
     7381 acatcaaggt actggctgga agaggtcgcc aggcacggga atgactttcg tatccacatg
     7441 gcggtaaata tcgcagggtg atgaatggtc tatagaggcc cggtgggtct ctggggcaag
     7501 ccggaagaaa gtgccgaaaa gttcagggta tttgaagtcg aaggccatcg ggacatcaaa
     7561 tgtcacatcg aggaagaggt cttccagtgg atggaggatg cggggctcat gccggaaggt
     7621 gcctacctct tcatggccgc g
//
LOCUS       DAOTIQ010000074         7530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_11726_length_7530_cov_6.518796, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000074 DAOTIQ010000000
VERSION     DAOTIQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11726_length_7530_cov_6.518796"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..574)
                     /locus_tag="P5541_08190"
     CDS             complement(143..574)
                     /locus_tag="P5541_08190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRV72002.1"
                     /translation="MAERTGAFSRPELDVLEEVLGEWAVKPEQGYRFDEVTGENGPAG
                     FVLWGKTPMTCRGYDIYWIVVDPSFQGKGYGKKMIALVEDQASQESPGCIIRLETSGK
                     EEYRTQRAFYLRCGFVEAGRIKDFYRDGDDLVTYAKTVTRN"
     gene            complement(540..1619)
                     /locus_tag="P5541_08195"
     CDS             complement(540..1619)
                     /locus_tag="P5541_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRV72003.1"
                     /translation="MRPDITVAVVAGREDTGREDVLDVLRCRESVIEGLTSRGVRAWP
                     VDITREDLLDENRVARKILGTSPSCVFNLFEGFSDASWLEIRFAGILEKMSIPYTGNR
                     SGTLALCLDKDLARKKLLQAGLPVAKGVCVSPGYDDSFVRELEFPLFVKPRMEDSSVG
                     IDARSFVENPEDLFRLLDERLASFPDGLIVEEFLEGREYNAGFAGYHPYELMAVSSID
                     YRFPEGAPAFLGYDSKWEPESSDYKNTASVAEESLDPSTRKGIIKISRMAGKVMGCSG
                     YFRVDLREHRGRIYILEVNPNPDINTDSGFAKQVARKGYGYGEMVERLARLAVNGRAP
                     YGKRQVREGERVPSHGGKDGCLLPT"
     gene            complement(1609..2694)
                     /locus_tag="P5541_08200"
     CDS             complement(1609..2694)
                     /locus_tag="P5541_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRV72004.1"
                     /translation="MKKEDPGSSFTDAGPVRRIGIAFNLKKKTAAAGETDDRYEEYDC
                     PETIQTLEREIRRLGFETELFEDDETFLDRISNNPPDFVFNIAEGRGSTRGRESQVPA
                     VLESLAIPFSGSDSVALAVTLDKWLTHHVLAGCGAAVPDLFMFSKAADLGRSIDLFEK
                     HGRYIVKPRWEGSSKGVFDDSVVDNPVDLAKRARRIWKNYRQPALVEEYLPGSEITVG
                     VMGNKNPAAIGMMLVQAEAAGSSAVYSIDHKRNWEERVRYLGPESIEPELRAILAAEA
                     VKVFRVLELRDIARVDFRLDGTGIPKTIDVNPLPGISETYSDLPILYRMSGGTFPSLL
                     KGILREALGRQGLRLPRASKTRQKNAA"
     gene            3037..>4724
                     /locus_tag="P5541_08205"
     CDS             3037..>4724
                     /locus_tag="P5541_08205"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRV72005.1"
                     /translation="MRPSKVLCLLAMIFAVILTVTLNPGISHSATRGETVDTVIRALG
                     LPLWGGGKHFTDLPPDHPFAASIETAAALGILHPSEQFYPDIDATRAEALMFSLQAMG
                     LRHEASLMGYFDKGAWPLMPSYIAPYLSLASEMEPSPPGQFLDDPAGSLSPADLRSLG
                     MWLRNCRQHMEWRKEIKGERSVLVLSRNNVGTPPSEWGVQSVEFGSEDEARTACDRLR
                     AMGFSALVHALEWTWVVRIGPFPHYLEAWETMMKIPSPEMTVVPFSQNTSHALFVAAL
                     AFDPAETPPRIVTAASVSGRRLPINIIAENSGAEGAINGGFFSGGRIIGSLVINSRPL
                     SGPYGDRSAAGWNLDGTGITFGRGDFRSTITLGKKTLPLSAMNSVPPQGGIAIFTPDV
                     WGYVTGAPPDGLEITVKNGTVAGVRPSAVSNHFVTREGFLVIARGYPARELQGVPDGT
                     PVSIGTEWAEPAFHGLDFILQAGPMLLREGKAQNSAEGFGPRTLSVPHPRSILGWDGS
                     KVWFIVIDGRDPWHRAGTTIAETAALIRRMGLTDALNLDGGGSSSIWWMGKTVTS"
     assembly_gap    4725..4824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5051..5359
                     /locus_tag="P5541_08210"
     CDS             5051..5359
                     /locus_tag="P5541_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRV72006.1"
                     /translation="MGKVIAEVVIAPHGTASPSLGKYVAEVEKTLRSFGLKTMLTPMG
                     TILEGDLDKVLEAVRAAHEIPFSQGALRVSTTLKIDERRDKEMTMEGKIRSVEEKMKN
                     "
     gene            complement(5431..5847)
                     /locus_tag="P5541_08215"
     CDS             complement(5431..5847)
                     /locus_tag="P5541_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72007.1"
                     /translation="MEELLVSRSETPFPKWMKWMALAVGMLLVGDGMRSFMFHKILVG
                     SVLAYVSGYEKRIFLSPEGIVRQTKTWIGAHSTLLPWDEVKFVNFAYKGSRMLCFFEK
                     DVTGLKVLFDRDDEPELLKILDEYIPEIERGVVGEQ"
     gene            complement(5885..7162)
                     /gene="ablA"
                     /locus_tag="P5541_08220"
     CDS             complement(5885..7162)
                     /gene="ablA"
                     /locus_tag="P5541_08220"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRV72008.1"
                     /translation="MQPRDRREIPLWKGVSDAEWNDWKWQVANRITTVEQLRQVIDID
                     DEEAEKVSRSLGKLRMAITPYYASLMDPKDPKCPVRIQAVPTEKETIIAKADLHDPLH
                     EDVDSPTPGLTHRYPDRGLLLITDQCSMYCRHCTRRRHAGETDKAYGEDQIRKGIEYI
                     RNTPTFRDVLLSGGDPLTIDDDRLEWVISELSNIPHVDFVRLGSRMPVVCPQRITDKL
                     CSMLRKYHPIWLNMQFNHPKEVTPETTKACAMLADAGVPLGNQSVLLKGVNDCPYLFR
                     ELNQRLLKIRVNPYYIYQCDLSEGIEHFRTSVGKGIQIIEYLRGHTTGMAQPLFIVDA
                     PGGGGKIPVGPNYVLSQSDRKLILRNYEGVLTTYTEPEDNISRPEELYHWEEYTARQR
                     SLSREGLIKLFEGELLSLEPAHLDRRERNEKGR"
BASE COUNT         1607 a         2115 c         2006 g         1702 t
ORIGIN      
        1 tgaacggtca acggtgaacg ggaaacccga ggcgtcagga gactggagac gggagacagg
       61 gaaggcaaga tccttaaatt caactgcgtg aacggggaac ggtgaacggg gtggggcaaa
      121 gggcaaagac cgtaacgcgt agctagttac gcgttacggt ctttgcatac gttaccaggt
      181 cgtcgccgtc gcggtagaaa tcttttatcc ttcccgcctc gacaaagccg cacctgaggt
      241 agaaggccct ctgggtacgg tattcctctt ttccggatgt ctcgagccgg ataatgcacc
      301 ccggcgactc ctgcgaggcc tgatcctcga ccagggctat catttttttg ccgtaccctt
      361 ttccctgaaa agacgggtcg acgactatcc agtaaatatc gtagccacgg caggtcatag
      421 gggtttttcc ccagaggacg aaccccgccg ggccgttttc gcccgtgacc tcgtcaaacc
      481 ggtaaccctg ctccggtttg acggcccact ccccgagtac ctcctccaga acgtccagtt
      541 caggtcggga gaaggcaccc gtcctttccg ccatgagaag gcaccctttc accttcgcgc
      601 acctgtcttt ttccataagg ggccctcccg tttacggcca gtctcgcgag cctctcgacc
      661 atctctccgt agccatagcc ttttcttgcg acctgtttcg cgaaaccgct gtcggtgttt
      721 atgtcggggt tgggattcac ctccaggata tatatgcgtc ccctgtgttc cctgaggtca
      781 acacggaaat atccgctgca tcccatgacc ttcccggcca ttcttgatat tttgatgata
      841 ccctttcttg tcgagggatc caggctctcc tcggcaacgg aagcggtatt tttataatcg
      901 ctgctctcgg gttcccactt ggaatcgtac cccaaaaagg ccggggcccc ttccgggaac
      961 cggtaatcta tcgaggatac cgccatcagt tcgtaggggt gatatccggc gaaacccgca
     1021 ttgtactctc tcccctcgag gaattcctcg acgatgaggc catcgggaaa agaggccagt
     1081 ctctcgtcaa gaagccgaaa aagatcttcg ggattttcca cgaaggatct ggcgtcaatt
     1141 cccacactgc tgtcttccat ccggggcttt acgaaaaggg gaaactccag ctccctgaca
     1201 aaagaatcat cgtacccggg ggaaacacat acccccttcg ctaccggaag cccggcctga
     1261 agcagtttct tcctggccag gtccttgtca aggcatagcg ccagggttcc agatctgttc
     1321 ccggtatagg ggatactcat cttttcgaga atgccggcaa accttatctc gagccaggac
     1381 gcatcactga agccttcgaa aaggttgaaa acacacgacg gagacgtccc gaggatcttt
     1441 ctggccaccc tgttctcgtc aagcaggtcc tccctggtta tatcaacggg ccaggcccgg
     1501 accccccggg atgtcaatcc ttcgatcacc gactcgcggc accggaggac atcgaggaca
     1561 tcttctctgc ccgtgtcctc tcttcccgcg acaacggcaa cggttatgtc aggccgcatt
     1621 tttctgcctt gtcttcgacg ctctgggcaa cctgagcccc tgcctgccta aggcttccct
     1681 cagtatgcct ttcaacaggg acgggaaggt ccctccgctc attcggtaaa gaatcggaag
     1741 atcgctgtac gtctcggata tcccaggaag aggattgaca tctatcgtct ttggaatacc
     1801 ggtgccgtca aggcgaaaat ccacacgcgc aatatcgcgg agttccagta cccgaaacac
     1861 ttttaccgcc tcagcagcaa gaatggcccg cagttcaggc tctatgctct cgggtccaag
     1921 gtaccgtacc ctctcctccc agttcctctt gtggtcgatg gaatatacgg cactcgatcc
     1981 cgcggcctct gcctgtacca gcatcatgcc tatggccgcg gggtttttgt ttcccattac
     2041 cccgacggta atttccgagc ccggaagata ttcctccaca agggctggct gcctgtagtt
     2101 tttccatatt cttcttgccc ttttagccag atctaccggg ttatccacga cggagtcgtc
     2161 gaatacgccc ttggacgagc cttcccacct tggtttgact atgtacctgc catgtttctc
     2221 gaaaagatcg atggatctgc ccagatccgc tgccttcgaa aacatgaaaa gatccggcac
     2281 cgccgcacca cacccggcaa gaacatgatg ggtcagccac ttgtccagag tcacggcaag
     2341 ggcaaccgaa tccgaaccgg aaaagggtat cgcaaggctc tcgagaaccg cgggaacctg
     2401 ggattccctt cctctcgttg aacctcgtcc ttcggctatg ttaaaaacaa aatctggcgg
     2461 attgttggaa atccggtcga gaaaggtctc gtcgtcctcg aacagttccg tctcgaaacc
     2521 gagccggcgg atctcccttt cgagggtttg tattgtttcg ggacagtcgt actcctcgta
     2581 gcggtcatct gtttcgcctg ctgcagcggt ctttttcttc aaattgaacg caatgccgat
     2641 ccgccggacc ggccccgcat cagtgaagga tgatccagga tcttccttct tcacttgttt
     2701 cgccccctgg ttttcttgat acactgcccg aacgggcaat gtcatgactg gtaggatgcc
     2761 ccttgatcca gccgttcgac cgacttcacc tccctcagga agaggactaa tggcgcatcc
     2821 ccgaaagcct cgatcttgcc gaaggacacg agcccggggg ccttttaagg ggcttgctgg
     2881 ccgtgtccgg gagatgcgct caaaagcact gatcctgtat tgatgctctc ttttgcggat
     2941 tatacatctc attttttgca atacaatagg ttcaacgaaa atacgcggac caatatttcg
     3001 acgaggcaga agacaatgcc ggaaggagag agaaaaatga gacccagtaa ggttttatgc
     3061 ctgcttgcca tgatctttgc ggtgatcctg acggttacct taaaccccgg catctcccat
     3121 tcggcaacaa ggggcgagac ggtagatacg gttatcaggg ccctcggtct tcctttgtgg
     3181 gggggcggaa agcattttac ggatcttccc cccgatcacc ccttcgcagc atcgatcgag
     3241 acagcggcag cacttggaat actgcaccct tcagagcagt tctaccccga catcgacgcc
     3301 accagggccg aggctcttat gttctccctg caggcgatgg ggctaaggca cgaggcctcg
     3361 ctgatgggct atttcgacaa gggagcatgg cccctgatgc cctcctacat tgccccctac
     3421 ctctcccttg cttcagagat ggagccttct ccgccggggc agttcctgga tgacccggcc
     3481 ggtagtttgt cccctgccga tcttcgctcc ctggggatgt ggcttcgaaa ttgccggcag
     3541 cacatggagt ggcgaaaaga gataaaaggg gaaagatctg tgcttgttct ctccagaaac
     3601 aatgtcggaa ctcccccctc ggagtggggg gtccaatccg tggagttcgg aagcgaggac
     3661 gaggccagaa ccgcctgcga cagactgcgg gccatgggat tttctgcact tgtccatgcc
     3721 cttgagtgga catgggtcgt aagaattggc ccgttccccc actacctgga ggcatgggaa
     3781 acgatgatga aaatcccttc accggaaatg acggtggtgc ccttttccca gaacaccagc
     3841 catgcccttt tcgtggcggc tcttgccttt gaccccgccg agacccctcc cagaatagtc
     3901 actgccgcct ccgtctcggg gagaaggctg cccatcaata tcatcgctga aaactcaggg
     3961 gcggaagggg ctatcaacgg agggtttttc agcggaggca ggataatagg ctccctggta
     4021 atcaactcaa gacccctgag cgggccctat ggggaccgtt ccgctgccgg gtggaacctg
     4081 gacggcaccg gtatcacttt cggcagggga gatttccggt cgactataac cctgggaaag
     4141 aagaccttgc ctctttcggc catgaacagc gttcctcccc agggaggaat tgccatcttt
     4201 acccccgatg tgtggggata cgtaacaggg gcaccaccgg acgggctgga gatcaccgtc
     4261 aaaaacggca cggtagcggg ggttcgccca tccgcggtgt ccaaccactt cgtcacacgc
     4321 gaagggttcc tggtaattgc aagaggctac cctgcccggg aacttcaggg ggtgcccgat
     4381 gggacccccg tctcgatagg gacagaatgg gccgagcccg ctttccacgg cctggacttt
     4441 atcctgcagg cgggtcccat gctcctcagg gaaggaaagg cacagaacag cgccgagggg
     4501 tttggccccc ggaccctttc cgtgccgcac ccgaggtcca tcctgggctg ggacggcagc
     4561 aaggtctggt tcatcgtgat agatggcagg gacccatggc atagagcggg gactaccatc
     4621 gccgagacag cggccctgat ccgccgcatg gggcttacag acgcgctgaa cctggacgga
     4681 ggcggctctt catccatatg gtggatggga aagaccgtaa catcnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnccttcc ccatgatctt gacaagcagt ttatccgcaa
     4861 cataccaacc cacgagaaca accagcggca tcagaaatct tccgaagtat ctttccatct
     4921 cgatccagtt catttaaaaa cctcccgcca gaatatagat ttgctgtgaa cgcggatctt
     4981 tattgattat acttgatgga attaaggtct ctgaaaacag gtaccggcca aatcggaagg
     5041 actggtgaaa atgggcaagg tcatcgctga agttgtaatt gctcctcatg gcacggcttc
     5101 ccccagcctg gggaaatatg ttgcggaagt tgagaaaact ctgaggagtt tcggcctcaa
     5161 gacaatgctt acaccgatgg ggacgatcct tgagggggat ctcgacaagg ttcttgaagc
     5221 agtgagggcc gcccacgaga tccctttcag ccagggagcg ctcagggtaa gcaccacgtt
     5281 gaagatcgac gaaaggcgcg acaaggaaat gacgatggag ggcaagatcc gttccgttga
     5341 ggaaaagatg aaaaactgac cgcttaaaaa gctatgaaag gcggagttgc tccgcctttc
     5401 atagctcggg ccaagttcaa caaaggggga tcattgctcc ccaactacac ctctttcgat
     5461 ttcgggaatg tactcatcca ggattttaag taactccggc tcatcgtccc tgtcgaaaag
     5521 aacctttaga cccgtaacgt ccttttcgaa gaaacagagc atcctgctgc ccttgtaagc
     5581 aaaattgacg aacttgacct cgtcccaggg caaaagagta gaatgcgctc caatccatgt
     5641 ctttgtctgc cggactatcc cttcaggcga gaggaatatc cttttttcgt accccgagac
     5701 gtaagcaagg accgaaccga ccagtatctt gtggaacatg aagctcctca tgccatctcc
     5761 tacgagaagc atcccgacgg ccagggccat ccatttcatc cattttggga acggtgtttc
     5821 cgagcgtgag accagaagtt cttccaaacc ctgcacctcc tgcaaaaatg cgcttttcgc
     5881 ggtactatct ccccttctcg ttcctctctc tgcgatccag gtgcgccggt tcaagggaca
     5941 ggagttcgcc ctcgaaaagc tttatgaggc cctcgcggga gagtgatctc tgccgggcag
     6001 tatattcctc ccagtggtac agttcctccg ggcggctgat gttgtcttcc ggttccgtgt
     6061 atgtcgtcag tacaccttcg tagttcctta atatgagttt cctgtcggac tggctgagga
     6121 catagtttgg tcccaccgga atcttcccgc cgccgcccgg ggcgtcgacg atgaagaggg
     6181 gctgggccat tcctgtcgtg tgcccgcgaa gatactctat tatctgtatc cccttcccca
     6241 cactcgtcct gaagtgctct atcccttcgg agaggtcgca ctggtagata tagtaggggt
     6301 tgacccggat cttgagtagc ctctgattaa gttccctgaa gaggtagggg cagtcattga
     6361 cacccttcag cagcacgctc tggttgccca ggggtacccc cgcgtcggcc agcattgcgc
     6421 aggcttttgt cgtctccggg gtaacttcct tcgggtggtt gaattgcatg ttcagccaga
     6481 tcgggtgata tttcctgagc atcgagcaaa gtttgtccgt aatcctctgt gggcacacca
     6541 ccggcatccg gctccccaga cgcacaaaat ccacgtgggg gatgttgctc agttcggata
     6601 tgacccactc cagcctgtca tcatctatcg taagcgggtc accgcccgag agaagaacat
     6661 cccggaaggt aggagtgttt ctgatatatt cgataccctt ccttatctga tcttcgccgt
     6721 aggccttgtc tgtctccccg gcgtggcgtc ttctcgtaca gtgacggcag tacatgctgc
     6781 actggtcggt gataagaagg aggcctctgt cggggtaacg atgggtcagt cccggcgtcg
     6841 gggaatccac gtcttcgtgc agcgggtcgt gaagatctgc ctttgcaatg atggtctcct
     6901 tctctgttgg aactgcctga atccttacgg ggcatttcgg gtctttcggg tccattagag
     6961 aggcataata aggagttatt gccatccgaa gtttacccag gctcctgctg actttttccg
     7021 cctcttcgtc gtcgatgtcg ataacctgcc ggagttgctc aacagtggtg atacgattgg
     7081 caacctgcca tttccagtcg ttccactcgg cgtcgcttac ccctttccag aggggtatct
     7141 ctcttctgtc gcgtggctgc attttttccc tcctgccaaa aataaaatta gtccgacttg
     7201 tatgcacaat cctgatttta tcctacaccc tctcgaagtg gagtgtcaaa cggggaaggg
     7261 caaggggaag ggcgtgaggg gtctccggga ggcggtccat caattcggaa tgttttccgc
     7321 agggagtggt ttgtaaacta gtcggttctc tgcataacgg ccggagcccg cccatgggag
     7381 tgagaggtga gaggtgagag gtgagacgaa aaggcgaggc ggcaggagtc gggagactgg
     7441 aggcagggaa ggcaggggca aaggcgtgaa cggtgaacgg tttaaaagcg aggcatcagg
     7501 agactggagg caggaaacag gaagggcaag
//
LOCUS       DAOTIQ010000075         7476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_11840_length_7476_cov_7.923056, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000075 DAOTIQ010000000
VERSION     DAOTIQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11840_length_7476_cov_7.923056"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..3535)
                     /locus_tag="P5541_08225"
     CDS             complement(182..3535)
                     /locus_tag="P5541_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72009.1"
                     /translation="MRRYEVAFILVCVFTALLVCFTDPVFARPVLPEFLQEKMNEVAL
                     FLESKGYEKGSTDSGKAKSSGFDVSADALRYTQWFTKTVEIKPKGSQGSLLDESYGES
                     VSDKVAITIYYPDWLNGGEACGSAEFPGVIGEAFAKVKNRFDAIDKENRSGENFSYSE
                     EIKRVVFSNAPDGNYELRQKWFRLSNGPAKGGWSVSEGSWSMFHQPWKDLDEYGLNAS
                     VRLYGTSLSSEVPFAFQIMAGRYEDTDVKPGGGGKLSEDLSRDGFWKPMRDNAEKVME
                     IILSETSPKPDTPAVSSDSPEPGLYLSIEAVPSSLPADGKSTSTLTVRTYDVDEKSRE
                     RKPLAGKMVDLSVAASNGTVPGTLAAKSVTTGKDGTATAKFTAPAQETIEGKKIAKAV
                     VTASSAGFNNDEVNIQLETFAPMKVKTEHQILPAGVSFENRITFSFGAPGENKVGGEV
                     KAVITVASKDGRLSAKGGQQGPYGEKIEISATPGAENHVYYHWSGEQPSEKAADEVVT
                     VEIPSMGLKGTVPFSVGVDLALTGGGPLYQGMIPGLFVPFKVYVHDRFHKDADLGAMF
                     QTFGIEPALQVVQTAFQPLSAMDSKEDFYTRLAAHVKGSVSPGTSLPWDILVGGVFRD
                     KDGGWLLVWKDYEENGLKDGSLPGLLPWSRGTYQAELRFDPKWKGDATEKDHVVKIAP
                     LVVEGKGQGDARIESFIIPILKSSVSLFPGGDAGLLAVDTAVELHKTGDVGKAAKEIG
                     RHYLLDFLSGKITDAVQPRLESDLEKRVWGTDRGKKIQERLTDLVKKANEGIKGLGKM
                     TEEQIDACIDYGKESLAGYFAGMFTETVLEPLKAGKSSLPTDVFVPFTTAIAWADGDP
                     AKEMVPLVSFLKGYGGSGIVLIEKKDGTLESVRSGETVLTPAPGQVFAYREKEQRVQD
                     TGKWLAVTLVKGESIVVSIKPGSDPVRLAHADGAGVKMTTIGKGAPLGESVRVSSSGF
                     AEGGSAPAAASVVSGIVTGSGVRIREDHSLEGKQVGTAGKGEKVEILEKWTSPSGMAI
                     IREDCIAGYDDIQYNLKKGMGVHVVKDAPGDEEENEEIIVTFTLDGKTVKAGVHIEFV
                     EVLEEQTWYRVRTEKGLEGWIFGKFIEEK"
     gene            complement(3728..4372)
                     /locus_tag="P5541_08230"
     CDS             complement(3728..4372)
                     /locus_tag="P5541_08230"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRV72010.1"
                     /translation="MEKIKVIAVLSLFIFAGILASGGISEAATHEDHLEVLLEGMPVG
                     VLTETEKSGILYLREEEKLARDLYAAFYEKWGVRTFSQISGSEENHMDSMKLLVDRYG
                     LKDPVLPEAGRFSDKKLQGIYDDLLAKGMVSRGEALKVAAMVEEMDIMDFREEQKKTD
                     KPDLLAVYESLERGSRNHLRAFGRQLNKESIRYEASFLSQEEVDAIMTSPNERD"
     gene            4583..5542
                     /gene="arcC"
                     /locus_tag="P5541_08235"
     CDS             4583..5542
                     /gene="arcC"
                     /locus_tag="P5541_08235"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRV72011.1"
                     /translation="MTTPTKVVIALGGNALQEAGTPPTAEAQLEVVGKTAQYLAEISC
                     KGYEMAIVHGNGPQVGRIVLTQEIASKENEQTPAMPFDVCGAMSQGYIGYHIQQALRD
                     ALRNRNRNIPVVTIVTQVVVDPKDPAFQNPSKPIGQFYTEEEAKKISEEKGYVMKEDA
                     GRGWRRIVPSPQPKRIVEISAVKRLWDTTIVITAGGGGIPVIENMDGSLTGVAAVIDK
                     DLAAEKLAQDIEADTLLILTEVDKVCLNFNKPEQKFLDHMTVPDAIKYMNEGHFAPGS
                     MLPKVLAAVKFARTYPGKKAIITSLYKAVDALEGKEGTVVTMA"
     gene            complement(5631..7346)
                     /locus_tag="P5541_08240"
     CDS             complement(5631..7346)
                     /locus_tag="P5541_08240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV72012.1"
                     /translation="MEQLQRNETTETTETGSFAGFKLRKELLKALETKGFTEPTPVQT
                     AVLGHDDLSKDLIVRARTGSGKTLAFLLPLMSDDRLDPSTTGVVVISPTRELAMQIAR
                     EATWVSRGMNFTCASLVGGMDMSGQIRDLRKGAAFAIGTPGRILDHIRRGTLKMDSVH
                     TVVLDEGDHMLDMGFREELESILSAAPQREKTWLFSATMPPAVRALSRKYLKDPKMIS
                     LVEDGAQHEDIVHTVYTTPRNRQMDGLVNVLLWENSPKGLVFCQTRAETIEVANRLTQ
                     ERFSSCCLHGDMSQRERNHALSMFVSGRVSHLVATNVAARGLDVPDVEHVFQFGLPSD
                     AETFIHRSGRTGRAGNEGRDILVLTLAESDKLRGMLRNTNIKINWLPVPDSQAIKTRQ
                     RARYEEKIFGVCSDGLCDTGEWAASLLERSDPVELVSNLLRIAERSIRSGYSLKDDLE
                     REKEQVRTRRLRPAPARGERGERGDKRNQGRRPKGTLVRLGVTNTSDWNAGKVLRTVC
                     SSLDVSSRDVAEIVLKKDSIMVELLPRALEKYRRNPESLSGASLSKIASSGRRDHTRP
                     GKVRS"
BASE COUNT         1590 a         2203 c         1955 g         1728 t
ORIGIN      
        1 atatacggaa cggaatatct caagaacgtc gaccccgaca catacgcgaa caggggggtt
       61 ctcctgaaga agggcatatt gatctcatcc tggtcggcaa cagtcacaag ggcataacaa
      121 cagaaaaagg ggaggcagga ataatccggc cgccccttta ctgctttccg gaactacctc
      181 gttacttttc ttctatgaac ttgccgaaga tccacccctc aagtcctttt tcggtcctta
      241 cccggtacca ggtctgttcc tccaggacct ccacgaactc gatgtggaca ccagccttga
      301 cggtttttcc gtccagggta aaggtcacga tgatctcctc gttctcctcc tcatcccccg
      361 gggcatcctt gacaacatga actcccattc cctttttcag gttgtactgt atatcatcgt
      421 aaccggcaat gcagtcctcc cttattatgg ccatgcccga gggggaggtc cacttctcca
      481 gaatttcgac cttttctccc ttgccggcag ttccaacctg tttcccttcg aggctgtgat
      541 cttcgcgaat gcgtaccccg ctgccggtca cgataccgga aaccacggac gctgcggcag
      601 gagcggaacc accctccgca aaaccggagg aagagaccct tacgctctct ccgagaggag
      661 cccccttgcc gatggtggtc atcttgacgc ccgccccgtc ggcatgggct agccttacag
      721 gatccgaacc cggcttaatg gaaaccacga tggattcgcc tttgacaagg gtcacggcaa
      781 gccatttccc ggtgtcctgt accctctgct ccttctccct gtaggcgaag acctgacccg
      841 gagcgggtgt aagaacggtt tctcccgacc tgacgctctc cagggttcca tctttcttct
      901 cgatcagaac gataccgctc ccgccgtaac ctttcaggaa ggagacgagg gggaccatct
      961 ctttcgccgg gtcaccgtcg gcccaggcga tggccgtcgt gaaggggacg aaaacatcag
     1021 tggggagaga gctttttcct gccttcagcg gttccagtac ggtctcggta aacattccgg
     1081 cgaagtaacc cgcgaggctc tccttgccgt agtcgatgca ggcgtcgatc tgctcttccg
     1141 tcatcttgcc gagcccttta atgccttcgt tggccttttt cacgaggtct gtcagccttt
     1201 cctggatctt ctttcccctg tcggtgcccc agaccctttt ttccagatcg ctctccagtc
     1261 tgggctggac ggcgtcggtg atcttgccgc tgaggaagtc caggagatag tgcctcccta
     1321 tttccttcgc cgccttgccg acatcgccgg ttttatgtag ttccaccgcc gtgtctacgg
     1381 ctaggagtcc ggcatcccct cccgggaaca gggagaccga acttttgaga atggggatta
     1441 tgaaactctc gatgcgagcg tcaccctgcc ccttaccctc gacaaccagg ggggcgatct
     1501 ttacgacgtg gtccttctcc gtcgcgtcac ccttccattt gggatcgaag cgaagttctg
     1561 cctggtaggt ccctctgctc cagggcagca gccccgggag cgatccgtcc ttaaggccat
     1621 tttcttcata gtccttccag acgagaagcc agcccccatc cttgtcgcgg aagaccccgc
     1681 cgaccaggat atcccaggga aggctggttc ccggagagac gctccccttg acgtgggcgg
     1741 cgagacgagt gtagaaatcc tccttcgaat ccatcgcgga aagaggctgg aaagctgtct
     1801 gtactacctg aagcgcgggc tctatgccaa aggtctggaa catcgcccca aggtcggcat
     1861 ccttgtggaa cctgtcgtgg acatacacct tgaaggggac aaacaacccg gggatcattc
     1921 cctggtagag cgggcctcca ccagtgaggg caaggtccac gcctaccgag aagggaacgg
     1981 ttcccttgag ccccattgag ggtatctcca cggtgacgac ctcgtcagct gccttttcgg
     2041 agggttgttc tccgctccag tggtagtaga cgtggttttc ggcaccagga gtagcgctga
     2101 tctcgatctt ttccccatag gggccctgct gcccgccttt ggccgaaagc ctgccatctt
     2161 ttgaggcaac ggtaattaca gccttgactt caccgccgac cttgttctcg ccgggagccc
     2221 cgaaagaaaa ggttatacgg ttctcgaaag atactcccgc cggaagtatc tggtgctctg
     2281 tcttcacttt catcggggcg aaggtctcaa gttgtatgtt cacttcgtca ttattgaacc
     2341 cggcagaaga ggctgtcaca actgccttag cgatcttttt gccctcgata gtctcctgtg
     2401 cgggagcggt gaattttgcc gtggcagtgc cgtctttgcc tgttgtcacg cttttggctg
     2461 caagcgttcc cggtaccgtt ccattcgagg ccgcgacgga aaggtcaacc atcttccccg
     2521 ccaggggctt ccgctcgcgg ctcttttcgt ccacatcgta tgtacgaacg gtgagggtag
     2581 atgtactctt tccgtcggcc ggaagcgaag atggaacggc ttcgatgctg aggtagagcc
     2641 ccggttccgg agaatcggaa gacaccgcag gggtatccgg cttcggggag gtttcagaga
     2701 ggatgatctc catgaccttt tcggcgttgt ccctcatcgg cttccagaaa ccgtcgcggg
     2761 agaggtcttc ggagagtttt cccccgcccc ccggtttgac gtcggtatcc tcgtacctgc
     2821 cggccatgat ctggaaggca aagggcactt cggaagaaag ggacgttccg taaagccgga
     2881 ccgaagcgtt cagtccgtat tcgtcgagat ccttccaggg ctggtggaac atggaccagg
     2941 aaccctcgct gacgctccac ccccccttgg cggggccgtt cgaaagcctg aaccatttct
     3001 ggcgaagttc gtagttgccg tcgggagcat tggagaagac aaccctcttt atttcctcac
     3061 tgtaagaaaa attttctccg cttctgtttt ctttatcgat agcatcgaag cgatttttta
     3121 ctttcgcaaa ggcttcaccg atgactccag gaaattccgc cgatccacag gcttctcccc
     3181 cgttcagcca gtccgggtag tatatggtga tcgcgacctt atcgctgact gattcaccgt
     3241 aggattcgtc aaggagactg ccctgggacc ctttcggctt tatctccacg gttttcgtga
     3301 accactgcgt atagcgcaga gcgtcggccg aaacatcgaa ccctgagctt tttgccttgc
     3361 ctgaatcggt tgaccctttc tcgtaacctt tgctctccag aaagagagcg acttcgttca
     3421 ttttctcctg gagaaattcc ggaagcacgg gtctggcaaa aacaggatcc gtaaaacaaa
     3481 ccagaagagc agtaaaaaca cagaccagga taaaggcaac ttcatatctt ctcaaaagga
     3541 acccctcccg atgaacagca aaatactccc gcgggcaaaa aaacagagtg gtttgtcccg
     3601 ttggctatat tatattcctc atgtcagaaa aaattgtttt ctaccggatc gccgaaaaag
     3661 atcatcgaca caaaaaaggc ccccgctgtt ctgggggccg gaccatgttt tgctatattg
     3721 cagcctgtca gtctctctcg ttgggggagg tcatgatggc atcgacctct tcctgcgaca
     3781 gaaacgacgc ttcgtagcgg atgctttcct tgttgagttg cctcccgaag gccctgaggt
     3841 ggttcctgga acctctttca aggctttcgt aaacagcgag aagatccggc ttgtcggtct
     3901 tcttctgctc ttcacgaaaa tccatgatgt ccatctcctc gaccatggcg gccaccttca
     3961 gcgcttcacc cctcgagacc atccctttgg caagaaggtc atcgtagatg ccctggagtt
     4021 ttttatcgct gaatctccct gcctccggca gcaccgggtc cttcaggcca tacctgtcca
     4081 caagaagttt catgctatcc atgtggtttt cctcgctacc cgagatctgc gagaaggtac
     4141 ggacacccca cttttcgtaa aaagccgcgt aaagatccct ggccagcttc tcctcctccc
     4201 tcaggtagag gatcccgctt ttctccgttt ccgttaaaac gcccacgggc atcccttcga
     4261 gaaggacctc tagatgatcc tcatgggtgg cggcttcgga aatgccgccg gaagcaagaa
     4321 ttccggcaaa aatgaacagg gaaagcaccg caataacttt tattttctcc actggtaacc
     4381 cctccttcaa atattccgca tacctactat acgggcgcag ggtataaaga gcatcgtaag
     4441 atttacctat tccgggaaca ggtagaactt gcctttctcg gacaacgaag gaactggaaa
     4501 aaaccggttg aacttttccg aagaaaaggt ttaaaatttt tccgtttcat ggatcttcca
     4561 ttcgagaagg tgaggtaaaa aaatgacgac gccgaccaaa gtcgtaattg ccctgggggg
     4621 aaatgccctg caggaggccg ggacccctcc gacggccgag gcacagctcg aggtggtagg
     4681 aaagaccgcg caatacctcg cggagattag ctgcaagggg tatgaaatgg ccattgttca
     4741 cggcaacggc ccccaggtgg gaaggatagt tctgacccag gagatagcct cgaaggagaa
     4801 cgagcagact ccggcaatgc cctttgacgt ctgtggggct atgagccagg ggtacatcgg
     4861 gtaccacata cagcaggctc tcagggacgc cctccgtaac cgcaaccgca atattcccgt
     4921 ggtcacaatt gtgacccagg tagtcgtcga ccccaaggac ccggcgttcc agaacccctc
     4981 caaacccatc gggcagttct acaccgagga agaggcaaag aaaatatccg aagagaaagg
     5041 ctacgtgatg aaggaggacg cggggcgcgg atggcgcagg atcgtcccct ctccccagcc
     5101 gaaaaggatc gtggagatct ccgcggtcaa gaggctctgg gacactacca tagtaataac
     5161 ggccggagga gggggaatcc ccgtcatcga gaacatggat ggctctctca cgggtgtcgc
     5221 cgcggttatc gacaaggatc tggccgccga aaaactggcc caggacatag aggccgatac
     5281 cttgcttatc ctcaccgagg ttgacaaggt ctgcctgaac tttaacaaac ccgagcagaa
     5341 attcctcgat cacatgaccg ttccggacgc cattaagtac atgaacgagg ggcacttcgc
     5401 tcccgggagc atgctcccca aggtccttgc ggcagtcaaa ttcgcacgca cctatcccgg
     5461 caagaaggcc ataataacct ccctttacaa ggccgtagat gctcttgagg ggaaagaggg
     5521 tactgtagta acgatggcat aggcaacact ctgccaaaaa gatgaaggcc ccgggaatct
     5581 caccggggcc ttcattattt tccttcaaca gtattacagg ggcggtcttt tcaggatcgg
     5641 acctttccgg gtctcgtgtg atccctgcgt cccgaggaag cgattttcga aagggacgct
     5701 cccgaaaggc tctcggggtt cctcctgtat ttttccagag cccttggcaa tagttcgacc
     5761 atgatgctgt cctttttaag gactatctcc gccacatccc tgctgctgac atcgagagaa
     5821 gaacataccg tcctcaggac cttgccggcg ttccagtccg acgtgttggt aaccccgaga
     5881 cgcaccaagg ttcccttcgg gcggcgcccc tggttcctct tgtccccgcg ctctccgcgc
     5941 tctccacgag ccggtgcggg ccttagcctt ctggtcctga cctgctcttt ctccctttcc
     6001 aggtcatcct tcagggagta tccggaacga atgcttcttt cggcgatacg cagaaggttg
     6061 gataccagtt ccaccgggtc gcttctttcg agaaggctcg cagcccactc gcctgtatcg
     6121 caaagcccat cggagcagac tccgaagatc ttttcctcgt accgcgccct ctgtcttgtc
     6181 ttgatggcct gcgaatccgg cacggggagc cagttgatct tgatatttgt attgcgcagc
     6241 atgccgcgca gtttatccga ctcggcaagg gtaaggacca ggatgtcccg tccttcattc
     6301 cctgccctgc cggtccttcc gctcctgtgt atgaaagtct ccgcgtctga aggaagcccg
     6361 aactggaaga cgtgctccac gtcgggcaca tcaagacccc ttgccgccac attggtcgcg
     6421 acaagatggg agaccctgcc cgaaacgaac atcgagaggg cgtggttcct ctccctctgg
     6481 ctcatgtcac cgtgcaggca gcaggagctg aacctctcct gggtcaggcg gttggctacc
     6541 tctatagtct cggccctggt ctggcaaaag acgagcccct tgggggagtt ctcccagaga
     6601 agaacgttga cgaggccatc catctgcctg ttgcgcggag tggtatagac tgtatggaca
     6661 atatcctcgt gctgggctcc atcctccacc agggagatca tcttcgggtc cttgaggtac
     6721 tttctggaca gagccctgac ggccgggggc atggtggccg aaaaaagcca cgtcttttct
     6781 ctctgcggag ccgcgctcaa gatagactcc agttcctccc ggaaccccat gtccagcatg
     6841 tggtcaccct catcgaggac aacggtgtga accgaatcca ttttcagcgt tcccctgcgg
     6901 atatgatcca ggatccttcc gggagtacct atggcaaagg cggcgccttt cctgagatcc
     6961 cgtatctgac ccgacatatc cataccgccc accagggatg cacaggtaaa attcatcccc
     7021 cgggaaaccc aggtggcctc cctggctatc tgcatggcca gttccctcgt cggggaaatg
     7081 actactactc ccgtggtcga agggtccaga cggtcatcgc tcatcaacgg cagcaggaac
     7141 gcaagggtct ttcccgaacc agtcctggca cggactatca ggtccttgga gaggtcgtca
     7201 tgcccgagaa cggctgtttg caccggggtt ggttcggtaa atccctttgt ttcgagagcc
     7261 ttcagaagtt ccttgcgcag tttgaatcct gcgaaggatc cggtttctgt cgtttctgtt
     7321 gtttcattcc tttggagctg ttccatataa tcactttcct cctgtatttg ggaaaggaca
     7381 caaaaaaagc ggcaccccac catggggtcc cacccgttat aatcccttca cggcaatagg
     7441 ggtattatgg cgactgggca ggatttgtca agggca
//
LOCUS       DAOTIQ010000076         6964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_12900_length_6964_cov_7.839485, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000076 DAOTIQ010000000
VERSION     DAOTIQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12900_length_6964_cov_7.839485"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="P5541_08245"
     CDS             complement(<1..185)
                     /locus_tag="P5541_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRV72013.1"
                     /translation="MNVFEIRKDIFWVGAIDWELRDFHGYSTERGSTYNAFLVMDEKK
                     TLMDSVKSTKTDELLTRV"
     gene            complement(182..730)
                     /locus_tag="P5541_08250"
     CDS             complement(182..730)
                     /locus_tag="P5541_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRV72014.1"
                     /translation="MDAMIDKRALYSLSYGLYLVCSRKGEKLNGQVANAVMQVTSEPV
                     SIIACLHKDNLTTECIRESRVFSVSVLEEETPMTFIGTFGFKCGRDVDKFKLCSHDAG
                     FTGAPVVLDHSIAAIEAKVVREVEIFTHVMFFGEVVASRFIKEAKPLTYAHYHEVKKG
                     KSPKNAPTFTLNEVEIAGEVKE"
     gene            complement(853..1347)
                     /locus_tag="P5541_08255"
     CDS             complement(853..1347)
                     /locus_tag="P5541_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRV72015.1"
                     /translation="MIGKKMQDALNRQINEELFSSYLYLAMSADFQTKNLTGAAAWMS
                     LQAEEERTHAMKFFHYLIERGGEVELLPIDKPQKSWKTPLDAFRDAEKHEQHITACIN
                     SLADLAVEEKDYATQNLLQWFVNEQVEEEASVGEVVAKLEMVSDSRNGLFMMDRELGK
                     RVAD"
     gene            complement(1352..1726)
                     /locus_tag="P5541_08260"
     CDS             complement(1352..1726)
                     /locus_tag="P5541_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRV72016.1"
                     /translation="MAKQLDVFKCDICGNVVELLHVGGGELVCCGKPMTLMEEKTADS
                     ATEKHVPVVEKKNGGISVKVGSVPHPMEEKHYIEWIEIMADGKICKKFLKPGEPAEAF
                     FEVDGKEVTAREYCNIHGLWRG"
     gene            complement(1948..3324)
                     /locus_tag="P5541_08265"
     CDS             complement(1948..3324)
                     /locus_tag="P5541_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRV72017.1"
                     /translation="MARKNLVYGVEDRPPTPIMILAGLQHVLTLFGATTLVPLIFGPA
                     MGMSGAQIGFFISCVYFAMGVATLIQTHPKLGSGLPIVQGSSFSFIPPIMTIIGAYKG
                     LGPAVVMQYIGGALISGGLILSGIGYSRLVGYVRKVITPIVIGPTIMAIGFSLAPVAI
                     QFNAANYWPVSLIVVACVFIFSLISRNKYMNIFAILSSIVIAYLVCLFGSMAGVFASG
                     HPAHVSLGEVVSAPWFRFKIFMPWGVPKFSFLAFGAIIAGFFAVMIESIGDYHSCSYA
                     AGLDDPDSDTISRGIGAEGLNCALAGLFGAVGTTSYTENIGLIGLTGVASRWVVRTGA
                     VLLIIMSFIGKLGALISTVPSPVIGGAYIALFGIIGALGIQVLMRADMGSQRNILIVG
                     FAFLMAMGLPGWTETQQEAFFAFGIPGQMFWAILKTPMAVAGISAAFWDSIVPGTPEE
                     RGIGVKMK"
     gene            3483..4241
                     /locus_tag="P5541_08270"
     CDS             3483..4241
                     /locus_tag="P5541_08270"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRV72018.1"
                     /translation="MDASVTKAEKCARLIKESQRIVLLSGAGMSTSAGLPDFRGPDGI
                     YRREMGVDPERIFDINWFIRDPSFFYGFHRDFLRSLEKIEPTFSHNFFAKLEETGKLS
                     GVITQNIDALHQRAGSKRVLEIHGSVWESACMSCGKTWDYEKSVSMAFSSNVPRCDSC
                     GGVVKPDVVFFGEMVKFLEESREMARNSDLFIVAGSSLVVTPAALLPSLSGGKIVVVN
                     RGNIEERNLPRARVQIFADEDIDTFFRTVERWLC"
     gene            4309..5343
                     /locus_tag="P5541_08275"
     CDS             4309..5343
                     /locus_tag="P5541_08275"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRV72019.1"
                     /translation="MNNRTVFTRYRAFTLVEILTAIIIIGILAGALMLVFPGKSDRAT
                     ATSIINDLRVLKSASTQFYASTGTWPTSLEEISSMVSRNLKLECADEICYDVALDING
                     NFIGVKAELSFAGSGVRSILAGMADNNGLFADTELLNKYSGGNMALSPVRSTRVLVNG
                     NGSQANLFTADLSNLDQFVILNGGWNIIDGTLRPDSTGPEKRIAFGETDWTDYTISAT
                     ARVVDGAVSRNSGYAIYYRADQQPGLRNPGISGYAFQFDTGYREFLVRVVNNGRESEP
                     LYRVPMPDNIDIFSPHDIDITVSGTNHSIKVDGKEVLNFDDSTFLSGSAGLRAWNVTD
                     VEFTSIEVRE"
     gene            5420..5881
                     /locus_tag="P5541_08280"
     CDS             5420..5881
                     /locus_tag="P5541_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72020.1"
                     /translation="MKKRTLFPFLASAAIFILAAPCLAGSVGVNKALFASSIETFGIY
                     EETGNRYKSGSTALIYLEVVDFMLAEANGTYSLDLSIDLSVIDSSGKEVGRQSDMLAW
                     EKTFKSKVNDVYFTLNVTFGGWPPGKYTLVVTVKDNMARTFNPVKMPVEIF"
     gene            complement(5945..>6964)
                     /locus_tag="P5541_08285"
     CDS             complement(5945..>6964)
                     /locus_tag="P5541_08285"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HRV72021.1"
                     /translation="IRNRASEGVFGYAQAPLSTFNSVSNWLGSVHGWKVDPEWLVSTS
                     GVMPAVGVAIRAFSNPGDGVIIQPPVYPPFFRIIEDNERVVVENPLVSQEGYFRMDFE
                     NLGALLSDPGNRIMLLCSPHNPVGRVWGKDELEKLGRLCVDTGTLLISDEIHADIVFD
                     PFRHTPTASINLSIAENTISCVSPSKTFNIPGLQTAYGVIQNRELREAFQLSLRATAI
                     GRHNSFGLVAGDAAYREGATWRSLMLEYIMANRDHALSFFRREMPWVGVCPPEGTYLL
                     WLDMSGSGLDPEEVTKRLIYKGRVVLDPGYWFGDEGRGWQRLNTACPRSVLDEGLKRI
                     LYTFS"
BASE COUNT         1731 a         1810 c         1689 g         1734 t
ORIGIN      
        1 accctggtca ggagctcatc ggtcttggtg gatttgacgc tgtccatgag ggttttcttc
       61 tcatccataa ccaggaaagc gttgtaagtg cttccccgtt ctgtagagta accgtgaaag
      121 tcccttagct cccagtctat tgccccgacc cagaaaatat ctttcctgat ctcgaaaaca
      181 ttcattcctt tacctcccct gcgatttcga cctcgttcag cgtgaaggtt ggtgcgttct
      241 ttggcgattt gcctttttta acttcatgat agtgagcata agtcaaaggt ttcgcctctt
      301 ttatgaacct cgaagccaca acctcaccga aaaacatcac atgtgtaaat atttcgactt
      361 cacgaaccac ttttgcctcg atagcggcaa tggagtgatc aagaactacc ggtgcccctg
      421 taaaaccggc atcgtgggag cagagtttga atttgtcgac atcccttccg cacttgaaac
      481 cgaaagttcc gatgaaggtc atcggcgttt cctcttccag aactgaaacc gagaataccc
      541 ttgattccct gatgcattct gtcgtcagat tatctttgtg gaggcacgca attatcgaga
      601 ccggttctga ggtcacctgc attacagcgt tggccacctg gccgttgagt ttttctccct
      661 ttcttgagca aacaagataa agaccgtagg aaagtgagta aagggccctt ttgtctatca
      721 ttgcgtccat gataacggtc ccccttagat ttttcaaaaa acacagctgg gcttcaacca
      781 taataaaggg ggacggggac ctgtgccccg ccccctcttt gaagaaagtc tatccctcaa
      841 tattatggag gatcagtctg cgaccctttt gcccagttcc ctgtccatca taaagagacc
      901 attcctggaa tcggagacca tttcgagctt tgcgacaact tctccgacag aggcctcttc
      961 ctcgacctgt tcattgacaa accactgcaa aagattctgc gtcgcatagt ccttttcctc
     1021 tacagcgaga tctgccagcg aatttatgca ggctgtaatg tgctgttcat gtttttcggc
     1081 atccctgaaa gcgtccagtg gggtcttcca gctcttctgg ggcttgtcta tcgggagcag
     1141 ttcaacctct ccacctctct caatgaggta gtgaaagaac ttcatcgcat gggttctttc
     1201 ttcttctgcc tgaagagaca tccatgcggc cgccccggtc aggttctttg tctggaaatc
     1261 cgccgacatg gcaagataga gatacgatga gaaaagctcc tcgttgatct gtctgttaag
     1321 tgcatcctgc atttttttgc ctatcatctc attagcccct ccagagtccg tgtatgttgc
     1381 aatattctct agcagtaact tccttgccgt ccacttcgaa aaaggcttcg gcaggttctc
     1441 cgggttttag aaatttcttg cagatcttgc cgtctgccat tatttcgatc cattcgatgt
     1501 agtgcttttc ttccatcgga tggggaacgc ttccaacctt gacagaaata cctccgtttt
     1561 tcttctcaac caccggaaca tgtttttctg tcgcggaatc tgccgtcttt tcctccatga
     1621 gagtcatcgg tttgccgcag cacacgagtt cgccgccccc tacatgaaga agttcaacca
     1681 cgtttccgca gatatcacac ttgaaaacat caagttgttt tgccattgtt cccacctcca
     1741 aaagttttta tcgaaccatt ttgtatttta ccacttctcc tttgcgatag tctctatgac
     1801 agactcttca gtatctgttt atttgtaagg ttttcaagtt atgcgtatat tttcacattg
     1861 tgtctttgtt ttgtcaacaa aaaacagggg ccttaaggcc cctgtttttt gtatccgatt
     1921 cattatgcga cccgtctcgc ctcaatgcta tttcattttt actccaatac cgcgctcttc
     1981 gggagtaccg ggaactatgc tgtcccagaa agcggcggag atcccggcga cggccatggg
     2041 tgtcttgagt atcgcccaaa acatctgtcc gggtatacca aaggcaaaga aggcttcctg
     2101 ctgggtctcc gtccatccgg gaaggcccat ggccataagg aaggcgaacc cgacgatcag
     2161 gatgttcctc tggcttccca tgtcagcgcg cataagtacc tggataccta gggcccctat
     2221 gataccgaaa agagctatgt aggctccacc gattacagga ctcggcacgg tcgaaataag
     2281 ggcaccaagt ttgccgataa aactcatgat tataagaaga accgctcccg tacgaactac
     2341 ccagcgggaa gcaacaccgg taagtccaat gagtcctatg ttctctgtgt aagaggtcgt
     2401 cccgacggca ccgaacaaac ccgcgagggc gcagttcagt ccttcggcgc cgatcccccg
     2461 gctaattgtg tcagaatccg gatcgtcaag gcccgctgcg taggagcatg agtgatagtc
     2521 ccctatggat tcgatcatta cggcgaaaaa tcccgctatg atagcgccaa aggccaggaa
     2581 actgaatttg gggactcccc agggcatgaa gattttgaac cggaaccatg gggcactgac
     2641 cacctctccc aggctgacat gagcaggatg cccggaagca aaaacccctg ccatacttcc
     2701 gaaaagacag accaggtagg cgatcactat tgacgaaagg atggcaaaaa tgttcatgta
     2761 tttattccgg ctaatgaggc tgaagataaa gacacacgcc acaacgatca gggatacggg
     2821 ccagtagttg gcggcgttga actggatggc cactggagcc agagagaagc cgatggccat
     2881 gatcgtcggc ccgatcacta tgggggtaat gactttcctg acgtacccga cgaggcgact
     2941 gtatcctatt ccggaaagga taagccctcc tgaaatgagc gctccgccta tgtactgcat
     3001 aacaactgcg ggaccaaggc ccttgtaggc tcctattatc gtcatgatcg gggggatgaa
     3061 actgaaactg gagccctgga ctatcggaag accggatccc agtttgggat gggtctgtat
     3121 cagggtcgca acacccatcg caaaatagac gcatgatatg aaaaagccta tctgcgcgcc
     3181 ggacatcccc attgcgggac caaagatcag cgggacaagg gtggtcgcgc caaaaagggt
     3241 caacacgtgc tgaagaccag caagtatcat aatgggtgtc ggtggcctgt cctctacacc
     3301 ataaaccaga ttttttcttg ccatggatca aagcacctcc tgtaatagtt ccactatagt
     3361 tctgaagaga attttagcac tttgtgtaac cggttgcata aaattatgat tattttttga
     3421 gtcacgggtg tgatattctt tatgacacga aagggctgat ttgtatggag ggaactcccc
     3481 caatggatgc ttctgtcact aaagctgaaa agtgcgccag gttgatcaag gaatctcagc
     3541 ggatagtgct tctcagcgga gccgggatgt caaccagtgc gggtttgccc gattttcggg
     3601 ggcctgacgg tatttatcga cgggaaatgg gagttgatcc tgaacgtatt ttcgatatca
     3661 actggttcat ccgggacccc tcattttttt acggtttcca cagggatttt ctgagatctc
     3721 ttgaaaagat cgagccaact ttctctcaca atttcttcgc gaagctcgaa gaaaccggaa
     3781 agctttcggg ggtgatcact cagaatattg acgccctcca ccagagagcc gggtcaaaaa
     3841 gagtcctgga gatacatggg agcgtatggg agagcgcctg catgtcttgc ggaaaaacct
     3901 gggattatga gaaaagcgtt tcgatggcct tctcctcgaa tgtgcctcgc tgcgattcct
     3961 gcggaggtgt cgtcaagccc gacgtggttt tcttcgggga gatggtaaaa ttccttgaag
     4021 aaagcaggga aatggctcgg aattccgatc ttttcattgt ggctggatca tcccttgttg
     4081 ttactccggc tgccctgctc ccctcccttt cggggggcaa gatcgttgtc gttaacagag
     4141 gaaatatcga ggaacggaat ctcccccgcg ccagagtgca aatttttgcc gacgaagata
     4201 ttgatacttt tttccgtact gttgagagat ggctctgtta agggagccgc attttctgtt
     4261 atgatttcaa atgaagccaa atctggccct tccgggaggg ttgaaacgat gaacaacaga
     4321 acagttttta ccagatacag ggcctttacg ctggtagaga tcctaacggc gattatcatc
     4381 atcggtatcc tggcgggagc cttgatgctt gtctttcctg gaaaatccga cagggctacg
     4441 gccaccagca tcattaacga ccttagagta ctcaaatcgg cttcgaccca gttctatgca
     4501 tctaccggaa catggccaac atcgcttgaa gaaatatctt ctatggtctc caggaacctt
     4561 aaacttgaat gcgccgacga aatctgctac gacgtggccc tcgacatcaa cgggaatttt
     4621 atcggcgtaa aggcagagct ctcctttgcc ggaagcggtg taaggtctat ccttgccgga
     4681 atggccgaca ataacggcct ctttgctgat acagagttgc tgaacaaata ctccggcggc
     4741 aatatggccc tctctcccgt cagatcgacg cgggttttgg tgaacggcaa cggaagccag
     4801 gcgaaccttt tcacggccga tctgtcaaac ctcgaccagt tcgtgatact aaacggcgga
     4861 tggaatatca tcgatggcac ccttcgcccg gacagcacgg gccccgaaaa acggatcgcc
     4921 ttcggtgaaa cggactggac agattacacc atttccgcca cagcgagagt tgtcgatggc
     4981 gctgtatcaa gaaacagcgg ctacgctata tactaccggg ccgaccagca gccggggttg
     5041 aggaaccccg ggatcagcgg ttacgcattc cagttcgaca cagggtacag ggaattcctt
     5101 gtcagggtcg tcaacaacgg gcgggaatca gaacccctgt acagggtgcc catgcctgat
     5161 aatattgata tcttttcccc tcatgatata gatatcacag tttcgggaac caatcattca
     5221 atcaaagtgg atggcaagga agtacttaac tttgatgact ctaccttctt gtcgggttca
     5281 gcgggactac gggcctggaa cgtaactgac gtggagttca cttccatcga agtaagggaa
     5341 tagggggtat accggagttc tggttaagtt atataattca ttacagttca ccaggttaaa
     5401 ccgaaaggag accagtgaaa tgaaaaaacg gaccctgttt ccctttttgg cgagcgcggc
     5461 gatctttatc ctggcagcac catgcctggc gggatctgtg ggagtaaaca aggccctctt
     5521 cgcaagcagc atcgaaacct tcggcatcta cgaggagacc ggaaaccgct acaagtcggg
     5581 atcgacagcc ctcatctacc tcgaggttgt cgacttcatg ctcgcggaag ctaacgggac
     5641 atactccctg gatctgagca tcgacctgtc tgttatcgac tcctcaggga aagaggtggg
     5701 aaggcagtca gacatgctgg catgggaaaa gaccttcaag agcaaggtca acgacgttta
     5761 tttcaccctg aacgtgactt tcggcggctg gccgcccggc aaatacaccc tcgttgtcac
     5821 cgtaaaagac aacatggcaa ggacgttcaa ccccgtcaaa atgcctgtag agattttctg
     5881 agatcactta aggaaactaa acctaagggg cgaaccgaaa ggatcgcccc tttctctacc
     5941 agatctagga gaatgtataa aggatccttt tcaacccctc atctaaaaca gatctgggac
     6001 aggcggtgtt gagcctctgc catcccctgc cctcgtctcc gaaccaatag ccagggtcaa
     6061 gcacgaccct ccccttgtag ataagccttt tggttacttc ttccggatca agccccgaac
     6121 cgctcatgtc aagccataaa aggtaggtgc cttcaggggg gcagacaccc acccagggca
     6181 tctcccgacg gaaaaaagag agagcatgat cccggtttgc catgatgtat tccagcatca
     6241 aacttctcca tgtcgcgcct tccctgtaag cggcatcacc tgcgacaagc ccgaagctgt
     6301 tgtgtcttcc gatcgcggtg gccctcaggg acagctgaaa ggcctctctc agctctctgt
     6361 tctggatcac cccataggcc gtctgtaatc caggaatatt gaaggtcttg ctcgggctca
     6421 cacatgagat ggtgttttcc gcgatagata agtttattga cgccgtcgga gtatgcctga
     6481 aaggatcgaa aacaatgtcg gcatggatct catcagagat aaggagagtt cctgtatcaa
     6541 cacagagcct tccgagcttt tccagttcat ctttacccca gacacgaccc acgggattgt
     6601 gaggactgca aaggagcatt atcctgtttc ccggatcaga aagcagggcc ccgagatttt
     6661 caaaatccat cctgaaatac ccctcttggg aaacaagagg gttttctaca acgactctct
     6721 cgttgtcctc aatgatcctg aaaaaggggg gatataccgg gggttggatt atcactccgt
     6781 cgcccggatt tgaaaaggca cgtattgcca ctccgacggc gggcattact ccagacgtcg
     6841 aaacaagcca ctccgggtct actttccaac cgtgaaccga accaagccaa tttgaaaccg
     6901 aattaaaagt tgacaaaggc gcctgggcat aaccgaatac accttccgaa gccctgttcc
     6961 gtat
//
LOCUS       DAOTIQ010000077         6932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_12985_length_6932_cov_8.659735, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000077 DAOTIQ010000000
VERSION     DAOTIQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12985_length_6932_cov_8.659735"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2813)
                     /locus_tag="P5541_08290"
     CDS             complement(<1..2813)
                     /locus_tag="P5541_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HRV72022.1"
                     /translation="MNEPERPRGIDLPLSENAARVLKERYLIKNEDGAVIEKPDEMFW
                     RVARFVAAAEEEPADDTIVKMFHDMMARLDFLPNSPTLMNAGRRGGQLAACFVLPIED
                     SMEGIFDSLKHMALIHKSGGGTGYNFSKLRPKGDIVSSTNGVASGPVSFMGMFDQATD
                     VVMQGGMRRGANMGILDVDHPDVFDFIKAKTEEGKLKNFNISIGATEKFMWAVENDDN
                     WDLLNPRTKEYVRTVKARDLFNTICEMAWKTGDPGMVFMDKINKDNNLAHLGDITSTN
                     PCVCGDTLVHTAEGPKRARNLCGRIVQLLLNGRVMPSTEEGFFRTGVKPVLRLTTREG
                     FSLRLTTDHPVLKVTESTRYRHRTEWAAAGSLKPGDGIMLNNHRKSPEWSGSGDIDQG
                     YLIGLLIGDGTIKKDKTVLSVWVPEERVSGDEDMGQGPRAVMSEAMKAVSTMPHRSDF
                     QGWIPVKGRREYRLASGALKNLASSLGMSPSCKTITPEMETSCSSQFTRGLLRGLFDA
                     DGSVQGDQKKGISIRLSQSSLTTLEAVQRMLLRLGIVGTIYRNRRAESSAILPDGRGG
                     MKAYNTKAQHELVISKENILFFAEKIGFADTDKQTRLDENLEGYKRSLNRERFTATVD
                     AIIPDGEEEVFDVKVPGENAFDANGLVVHNCGEQPLLPYEACNLGSINLANMVSGNDV
                     DWEKLRLTTRLAVRFLDNVIDVNQYPLPQIEEMVKKTRKIGLGIMGWADLLFRLRIPY
                     DSDEALNLAKKIMSSVTSIGRQESVELARTRGECPALPGSGMRNSTITTIAPTGSLSM
                     IADCSSGIEPVFALEFTKEVLEGKQFTYRNRYYAEALESGESPSVIERVFKTAHEIAP
                     EWHVRMQSAFQEHVDNAVSKTINLPSTATVEDVKKAYLLAWQSHCKGITVFRDGCRSG
                     QVLYVEKTAQQQTLPIPQVSEVE"
     gene            complement(2924..3841)
                     /gene="glsA"
                     /locus_tag="P5541_08295"
     CDS             complement(2924..3841)
                     /gene="glsA"
                     /locus_tag="P5541_08295"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRV72023.1"
                     /translation="MKDIISRAVESGRSVLKDGAVATYIPELGKADPGALAAAFCGID
                     GTTVASGDKETKITIQSISKVASLGLAISLLGEKALFSRVGVEPSADPFNSIMRLEMR
                     APHRPLNPMINSGAILVLSLLPFGDSNERLEAVLRTMRLLTANPRLGVNEGVAVSERD
                     TSDRNRSLGFFLKSVGSMQGDVENILESYFRQCAIETTVEDLAVMGATLANEGVNPVS
                     KEQVFSKRTSEILRSLMATCGLYDGSGDFAVRVGFPAKSGVGGGISGAIHGRGGVAAV
                     GPALDGRGNSIGGIRALEILSDTLDLRVL"
     gene            3978..4727
                     /locus_tag="P5541_08300"
     CDS             3978..4727
                     /locus_tag="P5541_08300"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRV72024.1"
                     /translation="MFFRSIFLSDLHLGARWSRPEPLREFLGRVQCDFLYLVGDVIDG
                     WKISSLSSLSESHREILRRFGTIANRARVTYIPGNHDDFLDRFLGGSIGKILFRDRVF
                     HRTADGRKFLVSHGDEFDRSLRISPSLNSLAERSYDLALWLNAGVNAMLPLVGMRKRH
                     MAAFLKRKFKGVAGFFSGFERRITEEARKAGMDGVICGHLHNPFIRNVRDVLFCNCGD
                     WVENCSAIVEHIDGRLEMLRLDGAGQLNQAS"
     gene            complement(4742..6292)
                     /locus_tag="P5541_08305"
     CDS             complement(4742..6292)
                     /locus_tag="P5541_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72025.1"
                     /translation="MKKFSFFSGIAISFLTVIFLSLASPAAAAEKWSWNVTSGGILET
                     DISGKRILPGNFLDRERSSLLIWDKDSSPEFRLFDLGDSGWEIKKSFEVDGALFESTP
                     WLAADIDGDGLDEILFFTEGFLVRLDLDQKISPVVYVLFPKKPISAVSVKGEGDPSQK
                     LVLAYHKPDERPADGPLPLWVGVWDFDESGCKFLWTDEGSLGLIDPGFAPTDSVVCAG
                     NVLNDGNRSILVWRSQSDVSPSRYLTLILEKERLTAGPEFSIVGGKMKKDGPHDETPP
                     FVVGDLLPLELEETTYLLATMVGSGFTFSKNIIQLDETGFTDLGPLSGSAPEDLFFPY
                     PAPGDPMGILRLIPGRNSQETVFETLSAGEKFQHPWETIKGEGFRIDAPQGSSLSYSD
                     LSTRIDLPRTPGTMLDERSATIMISRCDSSQECFDGFEEAESIKVNGVQYLVNRGIKW
                     EADMGGKRFFLEEYAAFRNGKCIRLTFRMMKKDLTGFTDDPPRPEPPEEETDTAVFRR
                     MLESFSPD"
BASE COUNT         1415 a         1934 c         1852 g         1731 t
ORIGIN      
        1 ctctcgacct ccgagacctg agggatcggc agcgtctgct gctgagctgt tttctcgacg
       61 taaagtacct gcccggagcg acaaccgtcc ctgaaaacgg taatcccctt gcagtggctc
      121 tgccaggcca gcagataggc ttttttgacg tcttccacag ttgccgtgga tggcaggttt
      181 atggttttgc ttaccgcgtt gtccacgtgt tcctggaagg cggactgcat gcggacatgc
      241 cactccggcg cgatctcgtg ggccgtcttg aaaactcttt cgatcacgga gggggactcg
      301 ccgctctcca gggcttccgc gtaataacgg ttgcgatagg tgaactgttt cccttccaga
      361 acctccttgg tgaattcgag ggcgaaaacc ggctctatgc ctgaagagca gtcggctatc
      421 atggataggg agcctgtagg ggctatcgtc gtgatggtag agttcctcat ccccgaaccg
      481 ggaagagcgg ggcattcccc cctggtcctg gcaagttcaa cactctcctg cctgccaatg
      541 gaggtaacag aggacatgat ttttttggcc aggttcagag cctcgtcgga gtcgtaaggg
      601 atccttagcc tgaaaagcaa atctgcccac cccatgatac ccaggcctat cttcctggtc
      661 tttttcacca tttcctctat ctggggaagg gggtactggt tgacgtcgat cacgttgtcg
      721 aggaaacgga cggccagcct ggtggtcagg cgaagtttct cccagtcgac atcattgccg
      781 gataccatgt tggccagatt gatgctcccg agattgcacg cctcgtaggg aagaagcggc
      841 tgttcgccgc agttgtggac cacgaggccg ttggcatcga aggcattttc tccggggacc
      901 ttgacgtcga agacctcctc ttcaccgtca gggatgatcg cgtccaccgt cgcagtaaat
      961 ctctccctgt taagacttcg tttgtaccct tcaaggtttt catcaagcct ggtctgcttg
     1021 tcggtgtcgg cgaacccgat tttttccgcg aagaagagaa tattttcctt cgatatcacc
     1081 agttcgtgct gggccttcgt attgtatgcc ttcatgccgc ccctgccgtc cggcagaatg
     1141 gctgaactct ccgctctccg gtttctgtat attgtgccga cgatgccgag ccgcaggagc
     1201 attcgctgaa ccgcctccag ggtagtaaga gagctctggc tcaggcgtat gctgatcccc
     1261 tttttctgat ctccctgtac cgatccgtcc gcatcgaaga gtccgcggag gaggcctctt
     1321 gtgaattgcg aagagcagga tgtttccatt tcgggagtta tcgtcttgca ggagggggac
     1381 atcccaagcg aggaagccag attttttaac gctcctgatg ccaggcggta ttccctgcgc
     1441 ccctttacgg ggatccatcc ctggaaatcg ctcctgtgcg gcattgtcga gacggctttc
     1501 attgcctcgg acattacggc ccggggtccc tgccccatgt cttcatcgcc ggaaaccctt
     1561 tcctccggca cccacacgga aaggacagtc ttgtcttttt tgatggtccc gtctcctatc
     1621 agaagcccga tcaggtagcc ctgatcaatg tcgccggaac ctgaccattc aggtgatttt
     1681 ctgtgattgt tcaacatgat gccgtctccg ggcttcaggc ttccggctgc ggcccattcg
     1741 gtgcgatgcc tgtacctagt gctctccgtc accttgagca cagggtggtc ggtggtgaga
     1801 cggagtgaaa atccttcacg tgttgtcagg cggagtacgg gtttgacccc ggtccggaag
     1861 aatccctctt cggtcgaggg catcacccgg ccgttgagga gcagttgaac aattctgccg
     1921 cagagatttc ttgcgcgctt tgggccctcg gctgtatgaa cgagagtatc gccgcagaca
     1981 cagggattag ttgacgtgat atcacccaaa tgcgctaaat tgttgtcctt gtttatcttg
     2041 tccatgaaga ccattccggg atcgcccgtt ttccaggcca tttcgcatat cgtattgaaa
     2101 aggtcccttg ccttgacggt gcgtacatat tctttggttc gggggttcag gaggtcccag
     2161 ttgtcatcgt tttcgacggc ccacatgaat ttttcggtgg cccctatgga aatgttgaag
     2221 tttttcagtt tcccctcttc ggtctttgcc tttataaagt cgaaaacgtc ggggtgatcg
     2281 acgtcgagga tccccatgtt ggcgccacgc cgcatccccc cctgcatgac aacgtccgtg
     2341 gcctggtcga acatccccat gaaagagacg ggacccgaag cgactccgtt ggtgcttgat
     2401 acgatatccc cttttggcct aagtttactg aagttgtacc ctgtgccgcc accggatttg
     2461 tggatgaggg ccatgtgttt gagggagtcg aaaatgccct ccatgctatc ctctatagga
     2521 agcacgaaac aggccgcgag ctgtcccccg cgacgtccgg cgttcatgag tgttgggctg
     2581 ttggggagga aatcgagcct ggccatcatg tcatgaaaca tcttgacgat ggtgtcgtcg
     2641 gcgggttcct cctctgcggc ggcaacaaac ctggccacac gccagaacat ctcgtccggt
     2701 ttttcgataa ccgcgccatc ttcgtttttt atcagatacc tctcttttag aacccttgca
     2761 gcgttttccg aaaggggcag atctattccg cgcggtctct ccggctcgtt catgtccttg
     2821 tctcctttgg attacattct cccgaatata cttgatacgg ggttaatctt taaatgaggc
     2881 tctatatata gggtatcata acgttggaaa tattacaccc cgttcaaagg acccgaaggt
     2941 caagagtatc agagaggatt tccagggccc ttatcccccc tatcgagttt cccctcccat
     3001 cgagcgccgg gcctacggca gcgacgccac ctcttccatg tatggccccg gaaattcccc
     3061 cgccgacgcc gctcttcgcc ggaaagccga ccctcacggc aaagtctccg gacccgtcgt
     3121 aaaggccgca tgtggccatc agggagcgaa gaatctccga tgtcctcttg ctgaaaacct
     3181 gctccttcga gacggggttg accccttcgt tggccagggt ggcgcccatt actgccaggt
     3241 cctcgacggt ggtctcaatg gcgcactggc gaaaatagga ttccagaatg ttttcgacat
     3301 ctccctgcat ggaaccgacg cttttcagga aaaaaccgag ggacctgttc ctgtcgctgg
     3361 tatctctttc ggagacggcg acaccttcgt tcacaccaag gcgggggttg gccgtcaaaa
     3421 ggcgcatggt ccgcaggacc gcctccagcc tttcattgct gtcgccaaag ggcaggagtg
     3481 agagcaccag gatcgcgccg gagtttatca tcgggttcag gggcctgtga ggcgcgcgca
     3541 tttccaggcg catgatggag ttgaagggat ccgcgctggg ttctaccccg acccgggaga
     3601 acagggcctt ttctccaagg agcgagatgg cgaggcccag agaagccacc tttgagatcg
     3661 actgaattgt gattttggtt tccttgtcgc ctgaagcgac agttgttccg tcgatgccgc
     3721 aaaatgccgc ggcgagagcg ccaggatccg cctttcccag ttcgggtata tacgtcgcaa
     3781 cggcgccgtc tttcaggaca ctccggccgg attcaacggc ccggcttatg atatccttca
     3841 aatcattccc tcctttgcgg gattcttccg gtgcaatgtg aattatactt tgaaaaaaca
     3901 tgcctgtttc gagtagaatc acttgtatgc gcaaaacaaa aagcgatttc cccgaaaatg
     3961 atcagcagga caagaaaatg ttctttcgct cgatttttct ttccgatctt cacctggggg
     4021 ctcgatggag ccgcccggag ccacttcgcg aatttctggg cagggtccag tgcgatttcc
     4081 tgtatctggt aggagacgtg atcgatggat ggaagatctc cagcctctcc agcctcagtg
     4141 aatctcacag ggagatactt cgccgtttcg ggaccattgc caacagggca agggtcacct
     4201 acataccagg caaccacgat gattttctgg atcgcttcct tggggggagt atcggaaaga
     4261 tcctcttccg tgacagggtc tttcacagga ccgccgatgg aagaaagttc ctcgtatccc
     4321 acggagacga gtttgaccgt tctctgagaa tatcaccctc gctcaattcg ttggcagaga
     4381 ggtcctatga tctggccctg tggctgaacg cgggagtcaa cgcaatgctc cccctcgtgg
     4441 gaatgcggaa acgccacatg gcggcgttcc tgaaaagaaa attcaagggt gttgctggtt
     4501 ttttcagcgg attcgagaga aggatcaccg aagaggccag aaaggcaggc atggacggag
     4561 ttatatgcgg acaccttcat aaccccttca tcaggaacgt cagggacgtt ctcttctgca
     4621 attgcggtga ctgggtggag aactgctcgg ccattgtgga gcatatcgac ggaagactcg
     4681 agatgctaag gctcgacgga gccggccaac tgaatcaggc cagttgacac ccatcaaccc
     4741 gtcagtcagg actgaaggat tcgagcatcc ttctgaaaac cgccgtatcg gtttcttctt
     4801 ccggtggttc gggcctggga gggtcatcgg tgaatcccgt cagatccttt ttcatcatcc
     4861 ggaaagtcag cctgatgcat ttaccattcc tgaaggcggc gtattcctcg aggaaaaaac
     4921 gcttccctcc catgtcggct tcccatttta ttccccggtt cacgagatat tgaactccgt
     4981 tgacctttat gctctctgct tcttcgaagc catcgaaaca ttcctggctg gagtcacatc
     5041 gggatatcat gatcgtcgcg ctgcgctcgt ccagcatggt tcccggggta cgcggcaggt
     5101 ctattctggt tgaaaggtct gagtaggaca gggaggaacc ctggggggcg tctatcctga
     5161 aaccctcccc ctttatcgtc tcccatggat gttgaaactt ttctcccgcc gaaagggttt
     5221 caaataccgt ttcctgactg tttcttcccg gtatgaggcg gaggatcccc atcgggtctc
     5281 ccggggccgg atatgggaag aaaaggtcct ccggggcaga gcccgataga gggcctagat
     5341 cagtaaaacc ggtttcatca agttgaatga tgtttttgga gaaggtgaaa ccggagccca
     5401 ccattgtcgc aaggagatat gtcgtttctt ccagttccag ggggagaaga tctccgacca
     5461 cgaaaggggg agtttcgtcg tgtggtccat ccttcttcat tttcccgccg acgatggaga
     5521 attcagggcc cgctgtcagc ctttcctttt caaggataag ggtcagatag cggcttgggc
     5581 tgacatcgga ttggctcctc cagaccagga tcgaccggtt tccgtcgttc aggacgttgc
     5641 ctgcgcaaac aacagaatcg gtgggagcga aaccggggtc gatcagcccc agagaaccct
     5701 cgtctgtcca gaggaacttg caccccgatt cgtcaaagtc ccagactccc acccagagag
     5761 gcaatggccc gtcggcgggt ctctcatccg gtttatggta ggcaaggaca agtttttgcg
     5821 atggatctcc ttcacccttt acagaaacgg cggagatggg ttttttgggg aagaggacat
     5881 aaacgacggg tgatatcttt tggtcaagat ccagtctgac aagaaagccc tctgtaaaaa
     5941 aaaggatctc gtcaaggccg tcaccgtcga tatccgcggc cagccatgga gtgctttcga
     6001 agagggcccc atccacctca aaagattttt tgatctccca cccggaatct cctaggtcga
     6061 agagcctgaa ttcggggctt gaatctttgt cccagatgag gagggatgat ctctcccggt
     6121 cgagaaaatt gcccggcaag attctttttc ccgaaatatc agtctcaagg atccccccgg
     6181 atgtaacgtt ccaggaccat ttttcagcag cggcagcggg agatgccaat gaaaggaata
     6241 tgacagtgag aaaggaaata gcgatcccgg agaaaaagga aaactttttc atcgaatcac
     6301 ctctttcttg aaatgattat accccaccct gccacaaaga aaacccttta ccgttgaccg
     6361 cttacgcctt tggttttgtt gtcatcgctg aggagcgaag cgatcgaagc ggaatccgcc
     6421 agcaggcgga cgttccgtgc cctttgccta ccggcgggaa tctggattgg ttttttgttt
     6481 ttcctgcctc ctgtatccag tctcctgatt ccttgccttt tcgattcacg actcacgact
     6541 cgcgattcac gaaaaccaga tccttcgctg cgctcagaat gacaatcact ttgcctctgc
     6601 cttttcgtct cacgtctcac gtctcacgcc cttgcccttc cggcccttgc tcctcacaaa
     6661 gaaaggtttt ctctgtgtgg ctttaccctc tgtgtcccct gtggtgagaa gggccggtcc
     6721 ttggccttgc cgcctcccgc ttttgcccat gccttcctgt cgccagacgc cagtctcctg
     6781 cctccctggt cttccgcttc acgtctcacg tctcacgtct cacgcccttg cccttccggc
     6841 ccttgctcct cacaaagaaa ggttttctct gtgtggcttt accctctgtg tcccctgtgg
     6901 tgagaagggc cgggggatcg ttttatgaga gt
//
LOCUS       DAOTIQ010000078         6745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_13448_length_6745_cov_7.703139, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000078 DAOTIQ010000000
VERSION     DAOTIQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13448_length_6745_cov_7.703139"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5541_08310"
     CDS             <1..459
                     /locus_tag="P5541_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72026.1"
                     /translation="APAPAPEVKTQEAPVIEDTTTKETVKTIKEPAPTEAPKEEPAPA
                     PAPAPEVKTQEAPVIEDTTTKETVKTTKEPEPKEAPKEEPAPAPIKPVKDDVVSDDVR
                     IEEPATSPEPAPDDGTRTQSETPTEKEEEKPKIPPVDKPVSDDIADSVSE"
     gene            632..1330
                     /locus_tag="P5541_08315"
     CDS             632..1330
                     /locus_tag="P5541_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV72027.1"
                     /translation="MDNLRTQVPERLEDYLEKIFEFEIEGIEPTITGLAGRLGITKGA
                     TTLSVKRMVSMGLLNHEHYGQLQLTEEGYKRGLEIYRRHKHLSFLFSEMLGIPRKEAE
                     AVACAMEHSMREESEQRLLAFAEFYCTSRRNREEWTERLQQAIENHSCLPRPLPLLAP
                     GDKARIIRITALNPVREKLLSEGLFPDNAITFLQMTREGVAQLVLGEKQISVKRGEAA
                     SIWVCPLDCRVTCS"
     gene            complement(1535..2323)
                     /locus_tag="P5541_08320"
     CDS             complement(1535..2323)
                     /locus_tag="P5541_08320"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRV72028.1"
                     /translation="MEEKWMKRFFVRPALFAVLALATVATFAVSDTAWAHFQMVYTPH
                     AIADTGQELNLKIVFTHPFTAGHTMDMGKPRAFFLEHKGKRTDLGGSLKAINWKSMTN
                     QGVAYEAVYKVRTMGDYIFCLEPEPYLEESEDCYIQQFTKSYVNVAGMATDWDREIGL
                     PAEIVPLDKPYALWAGSTFRGIVKSGGKIVPYADIEVEFLNHQPLTKENAFSETAEVE
                     PPFSVMETLTIKADGNGVFGFWHSEGRLVGICRAGCRSGNRTQG"
     gene            complement(2435..2755)
                     /locus_tag="P5541_08325"
     CDS             complement(2435..2755)
                     /locus_tag="P5541_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72029.1"
                     /translation="MKTKNLVAAVVAVLVVSVAGIVFAHTPIFSCFLNEDGTVMCEGG
                     FSDGSSAAGVTVRVFDIQKKVLMEGEMDEFGTFVFNKPEVDYFVVMDAGPGHTVEIKG
                     DNIQ"
     gene            complement(2761..5253)
                     /gene="feoB"
                     /locus_tag="P5541_08330"
     CDS             complement(2761..5253)
                     /gene="feoB"
                     /locus_tag="P5541_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRV72030.1"
                     /translation="MKVAETKKRVVALAGQPNCGKSSVFTMLTGVHQHIANYPGITVD
                     IKKGKLTHDGGFSVEIVDLPGMYSLTSFSEEERAASDFLLEHRPDRIVNVVDSSNLSR
                     HLYLTLQLIETGVPLLLDMNMADVAKSHSIEIDGSLLSRVLGVPVVFTEAHREKGKKE
                     LISGILEEIPISARATVSVDYGPLETVIGEVSKALESSCDNMPLGSRWMALKLLEEDE
                     NIWRVFNSIPGSEHALELARAGVDNAITALGVPPAQFIALKRHEKAREIENRVSKKGE
                     RVESLSEKVDRFVLHPVAGLFVLAGVMFSLYELAIVQGYRLTSYSWPLLSWIRDHIAG
                     MLPGERLLVDPFLRTAVLYVVDGVMAVVNYVPIFLILFALVAVLEDGGYMPRMSFVLD
                     RLFRRFGLHGQSVLPLVLGGVFVGGCAVPGVMATRGIPDRRARLATIMVVPLMNCLAK
                     VPFYALLVSIYFPATQSMAMFFIATITLIVALIVSKVLTLTVLKHEPSAPFMMEMPSY
                     HLPALRHVSRRSLHRTWMFLRKIFTIVVVASALIFFLTQYPGLDETVVKNLDAGMNDA
                     ISVLGEQTAGTRYKAVFEDREQISAVAEYWQRYRKALMIAGGDRNARNRVTEEFRQEN
                     PVFVEIVTAKTDMDAVKANRALVAFMRTRMELRSEHGKLQVRNSFMGKAGSWLEGVTR
                     FAGFNWRINVALLSSFAAKENVVATLGSLYKPSEAGERLEERMAREEGAMTPLHALAM
                     MVFMALYPPCIATLLVVLSESGSLWWMILTLLYPLLLGLLFAILIFTGGSVLGLTGVQ
                     AMAVFYVSMLVVAFLVSRISDPEEKKILNEGR"
     gene            complement(5250..5471)
                     /locus_tag="P5541_08335"
     CDS             complement(5250..5471)
                     /locus_tag="P5541_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRV72031.1"
                     /translation="MRLRDMKPGERGVISGVEHARGMQRLLDLGFFPGVKVLVVRNAP
                     FKDPMECNLDGRRVSVRRQEVEGVEVRPE"
     gene            complement(5584..5805)
                     /locus_tag="P5541_08340"
     CDS             complement(5584..5805)
                     /locus_tag="P5541_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRV72032.1"
                     /translation="MCSLHECPSRCRAKIISVQGGRSLQRRCCDMGLIPGSEITVQMN
                     YGGRMHINCRGASYAVGGGMAEKIIVSRE"
     gene            complement(5926..6636)
                     /locus_tag="P5541_08345"
     CDS             complement(5926..6636)
                     /locus_tag="P5541_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72033.1"
                     /translation="MKKISIFAAVCFAVTVLSGFSAVAQDAGIYLEKRSFLPGEQISV
                     EFTALADFAYDAWVGIIPSEIPHGDESINDRYDLQYHHLENRTGGILLFVAPGSPGSY
                     DLRMHDTDKNGREVASVSFQVTGEGSAVAPSLTLERTVFIPGEEIVVTFTAPSTFASN
                     AWVGIIPSNIPHGDESRNDKYDISYLYLNNLTGGVLEFKAPPSEGKYDLRMHDTDNNG
                     REVATVSFTVSAAGLTKQ"
BASE COUNT         1835 a         1870 c         1503 g         1537 t
ORIGIN      
        1 gctccggctc ccgctcccga agtcaagact caggaagcac ctgtaatcga ggacactaca
       61 acaaaagaaa cggtcaagac aataaaggaa cccgcaccaa cagaagcacc aaaggaagag
      121 cctgcaccag ctccggctcc cgctcccgaa gtcaagactc aggaagcacc tgtaatcgag
      181 gacactacaa caaaagaaac ggtcaaaacg acaaaggaac ccgaaccaaa agaagcacca
      241 aaggaagaac ctgcacctgc tccgataaaa cccgtcaagg atgatgtcgt ctccgacgat
      301 gtgcggatag aggaacctgc caccagcccg gaacccgcac ctgacgacgg aacccggacc
      361 cagtcggaaa ctccgactga aaaggaagag gaaaaaccca aaataccgcc cgttgataaa
      421 cctgtatcgg acgacatagc agattctgtt tccgaataaa gcatctacca taaaaagggg
      481 ggcaagcgga aaaggcagcc cccctttacc tgtaaaaacc cgggatcaac ctgtttcctc
      541 tgatggaccc ggatgttacc ctgttcaagg gatttaaaga ctccccatgc tacaattcta
      601 tacaaggtaa ttacaagaag ggacaaacca tatggataat ctgagaaccc aggtacccga
      661 aagacttgaa gattacctgg aaaagatttt tgagttcgag atcgagggga tcgaacctac
      721 gataacggga cttgcaggga gattggggat aactaagggc gcaacaaccc tttcggttaa
      781 acgcatggtg tccatgggcc ttttgaatca cgagcattac ggccaactgc aacttaccga
      841 ggagggttac aaacgggggc ttgagatcta ccgcaggcac aaacaccttt ctttcctatt
      901 ctctgaaatg ctgggcattc caaggaaaga agcagaagct gtggcttgtg ccatggaaca
      961 ctccatgcga gaagaatccg aacaaagatt gcttgctttt gcggagttct actgcacctc
     1021 gaggagaaac cgcgaagagt ggaccgaaag actgcaacag gccatagaga atcactcctg
     1081 tctcccgcgc cccctcccgc ttctggcacc aggagataaa gccaggatca ttcgtattac
     1141 agcattgaac cctgttcgtg aaaaattgct ttctgaaggt cttttcccgg acaatgccat
     1201 cactttcctg cagatgaccc gtgagggcgt cgcccagtta gttctggggg aaaaacagat
     1261 cagtgtaaaa agaggagaag ctgcctctat atgggtttgc cccctggatt gccgggtaac
     1321 ttgctcctga tatttccgag gtaatataaa cggggggggc gccggacgaa tgacatacgc
     1381 ccggcgcttt tttgaagaca aagaggagaa tttactgatt gaattgaggc aagttacttc
     1441 aaaacaggcc gatataaacg ataatcatgg gctatccccg ttttatttca taccatgggc
     1501 atatacccaa attacggcat cctgtgaaag ttccttaccc ttgtgttctg tttccggacc
     1561 gacacccagc gcggcaaatc cccaccagcc ttccctcgga atgccaaaaa ccaaaaacac
     1621 cgttgccatc cgccttgatg gtaagggtct ccatcaccga gaaaggaggt tccacttcgg
     1681 cagtttcaga gaaagcattt tctttcgtaa gaggctgatg gttaaggaac tccacctcga
     1741 tgtcagcata aggaactatc ttgccgccac tttttacgat cccccggaaa gtgctacccg
     1801 cccaaagggc atagggtttg tcaaggggaa cgatctctgc aggcaggcca atttccctgt
     1861 cccagtcagt agccattccc gcaacgttta cgtaactttt cgtgaactgc tggatgtagc
     1921 agtcctcgct ttcctcaagg tagggctccg gttcaaggca gaaaatgtaa tcgcccatgg
     1981 ttcggacttt gtaaacggct tcataagcca caccctggtt agtcatactt ttccagttta
     2041 tcgccttgag gcttccgcca agatccgttc tcttcccctt gtgttccaga aagaaggccc
     2101 tgggtttccc catgtccatg gtgtgccccg ccgtgaaggg gtgggtgaac acaatcttca
     2161 ggttcaattc ctggccggta tccgctatgg catgcggtgt atagaccatc tggaaatgcg
     2221 cccatgcagt atccgagacg gcaaaggtcg caaccgtcgc aagagcaaga accgcaaaga
     2281 gcgctggccg cacgaaaaaa cgcttcatcc atttttcttc catcttgtga tatcatcctt
     2341 tcatatcgtt accacgtaat gctttgacgt gcgacgtccc tccccacggg cgtcgcacgt
     2401 aaccgccctt ccctctgtgt ttcacatccg gatatcactg gatgttatcg cccttgatct
     2461 cgactgtatg tccgggcccg gcatccatta ctacgaaata atcaacctcg ggcttgttga
     2521 aaacaaaggt gccgaattca tccatttcac cttccataag aaccttcttt tggatatcaa
     2581 acacccttac cgtgacaccg gctgcggaag aaccatcaga aaaacctcct tcacacatca
     2641 ccgttccatc ttcatttagg aaacagctga aaatcggcgt atgtgcaaaa actatccccg
     2701 caacagaaac caccagaaca gccacgactg ccgctacgag atttttcgtt ttcatatctc
     2761 tcacctccct tcattcaaga ttttcttttc ttcaggatcg ctgatcctgg aaaccagaaa
     2821 cgcaacaacc agcattgaaa cgtagaaaac cgccatagcc tggaccccgg ttaatccaag
     2881 gactgatccc ccggtaaaga tcaggatcgc gaacagcaga cccagcaaaa gaggatacaa
     2941 aagggtcaga atcatccacc aaaggctgcc cgattcactc agcaccacaa gaagggttgc
     3001 aatacacgga ggatacaggg ccataaaaac catcatcgca agagcatgca agggcgtcat
     3061 tgccccttct tctcgagcca ttcgctcttc cagccgctct cccgcttctg aaggtttgta
     3121 cagggaccca agagtggcaa caacgttttc cttcgcagcg aaggaactca aaagagccac
     3181 attgatcctc cagttaaaac ccgcgaacct ggtcacgcct tccagccagc tcccggcttt
     3241 acccataaaa ctgtttctga cctgaagttt gccgtgttca ctgcgaagtt ccattctcgt
     3301 gcgcataaac gcaacaagag ccctgtttgc tttaaccgcg tccatatctg tctttgctgt
     3361 cacaatttca acgaacaccg gattttcttg ccggaactct tcggtgaccc tgttccgggc
     3421 atttctgtca cctcccgcaa tcatcaacgc cttgcgatac cgctgccaat attcagcaac
     3481 tgcagatatt tgttccctgt cctcgaatac cgctttgtag cgagttccag ccgtctgttc
     3541 gccaagaaca gaaatggcat cattcatacc ggcatccaga tttttcacga ccgtttcgtc
     3601 aagccccgga tactgggtga gaaagaaaat cagcgcagac gcaacgacaa caatcgtgaa
     3661 aatttttcgg aggaacatcc aggtcctgtg gagagaacgt cgagaaacgt gccgcaacgc
     3721 aggaaggtga taggatggca tctccatcat gaaaggagcg ctgggttcgt gtttcagaac
     3781 agtgagggtc aggaccttgc tgacaattaa tgcaactatg agtgtaatcg tggctataaa
     3841 gaacatggcc atgctctggg tagccggaaa atatatactg accagaagcg cgtaaaaagg
     3901 gaccttggcg agacagttca tgagaggtac caccattatc gtggcgagac gagcccgcct
     3961 gtctggaata ccccgcgtag ccatgacccc tggaaccgca cagcccccga caaatacccc
     4021 accaagaaca aggggaagaa ccgactgccc gtgaaggcca aaccttctga aaagtctgtc
     4081 aagaacaaaa gacattctcg gcatgtaccc cccgtcttcc agaacagcga caagggcgaa
     4141 aaggatcaga aatatcggaa cataattgac aaccgccatc acaccatcga caacatacag
     4201 aactgctgtt cgaaggaaag gatcgacaag aagcctctca cccggaagca ttcccgcaat
     4261 atgatctcgg atccatgaaa gcaagggcca actgtagctt gtgagcctgt atccctgtac
     4321 aattgccaac tcataaaggg agaacatgac accagcgagc acaaacaacc cggctaccgg
     4381 atgtagcaca aagcggtcga ccttctcgct cagggactca acacgttccc ccttttttga
     4441 gaccctgttc tctatctctc tcgccttttc atgtcttttg agcgcaataa attgtgccgg
     4501 gggaactcca agggcggtaa ttgcgttatc caccccggcc cgggccagtt ccagtgcatg
     4561 ctcagatccc gggatactat tgaatactct ccagatgttt tcatcctctt ccagtagttt
     4621 cagcgccatc catctcgaac caagtggcat attgtcacaa gaggattcca gcgccttgga
     4681 gacttccccg ataacagttt ccaaaggacc ataatcaacg gatacggtag ctctggcgga
     4741 tatcggaatc tcctccagaa ttccggatat caattccttt ttcccttttt ccctgtgggc
     4801 ttctgtaaaa accactggaa caccgagaac ccgcgagaga agactgccat cgatttcgat
     4861 agaatgactt ttcgcaacat cagccatgtt catatccagt aaaagcggta ccccggtttc
     4921 aattaattga agggtcaggt acaaatgcct ggaaagattg gaggaatcta ccacattcac
     4981 gatcctgtcc ggccgatgtt cgaggagaaa atctgaggcc gcacgttcct cctccgaaaa
     5041 ggaggtaaga ctgtacatgc cgggaaggtc gacgatctcc acggagaatc caccgtcatg
     5101 ggtcaatttg ccttttttga tatcaacagt gatcccgggg tagttcgcaa tatgctgatg
     5161 aacgccggtc aacattgtaa acacagaaga tttcccacag ttgggctgcc ctgcaagagc
     5221 tacgaccctc ttttttgtct ccgcaacctt cattcaggcc ttacctccac tccttccact
     5281 tcttgccgcc ggactgaaac acgacgccca tccaggttac attccatggg atctttaaag
     5341 ggagcgttcc tgacaaccag aacctttacc ccaggaaaaa aaccaagatc caaaagtcgc
     5401 tgcatccccc tggcgtgttc aactccagag attactcccc gctcgccagg cttcatatcc
     5461 ctgagccgca taaacttcac cttccctgca agtaatatcc gcaaaatcat aaggaaaagg
     5521 aaaaatgttt tgttatttaa actctacaca ggacaaaaaa aattggcctt gttccggccc
     5581 tgtttattcc ctggagacga tgattttttc cgccatgcct cctccaaccg cataagatgc
     5641 gcccctgcag ttgatatgca tccttcctcc ataattcatc tgaacggtta tttcagatcc
     5701 cggtataagc cccatatcgc aacaacggcg ctggagcgac cttcctccct ggactgaaat
     5761 tatctttgcc ctgcatcttg acggacattc gtgtaatgaa cacatcaatc tggattcacc
     5821 ctttcggaaa aggctttgtt tatgtttagt aatataaact ttatggaatg ttacacctcc
     5881 tctttctaca tgtcaatacc caagatcacc agacgccggc aatactcatt gtttggtcag
     5941 acccgccgcg cttacggtaa aagaaaccgt cgcgacctcc ctgccattat tgtcggtatc
     6001 gtgcatgcgc aagtcatatt tcccttcact gggaggcgcc ttgaattcca gcacccctcc
     6061 ggtcagatta ttcaagtaga ggtaggaaat atcgtatttg tcatttctgg attcgtctcc
     6121 atgtgggatg tttgagggga ttattcccac ccaggcgttt gaggcaaagg tcgaaggggc
     6181 ggtaaaggtc acaacgatct cctcgccggg aataaaaacc gttctctcaa gcgtgaggga
     6241 tggtgcaacc gcagagccct cccctgtcac ctggaaagaa acagaggcaa cttctctgcc
     6301 gtttttgtcc gtatcatgca ttcgaagatc gtaagacccg gggctgcccg gagcgacaaa
     6361 caaaaggatt cccccggtcc ggttctcaag atgatgatat tgaaggtcat acctgtcatt
     6421 gatcgattcg tcgccgtggg gtatttccga gggtattatc cccacccagg cgtcatacgc
     6481 gaaatcggcc aaagcagtga attccacact gatctgctcc ccgggaagga aagatctctt
     6541 ttcaagataa atacccgcat cttgggccac ggccgaaaaa ccggacagaa ctgtcacagc
     6601 aaaacagacc gccgcaaaaa tcgaaatctt tttcaacgcg atcaccccct taaaaatttt
     6661 tctgctggtc caggcacaga atgcctgata tcccaaagtc ccccggctcc ttctgcattg
     6721 tatcaccgct ttttacgggt gcgtc
//
LOCUS       DAOTIQ010000079         6723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_13500_length_6723_cov_7.279094, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000079 DAOTIQ010000000
VERSION     DAOTIQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13500_length_6723_cov_7.279094"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="P5541_08350"
     CDS             complement(<1..1244)
                     /locus_tag="P5541_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRV72034.1"
                     /translation="MKENTENLRKLINQGEGLALEFKESRNGLNRNVYETVCAFLNRN
                     GGTLLLGVRDSGEILGIDPGKLEHIKKDFITSINNPQKIHPTCYLSVDELELGGKRIL
                     GIYVPESSQVHRCSGRIFDRNEDGDLDITDNTLLVAELYRRKQSTYSENRIFPYAKMS
                     DLRTDLIDRARKMAGIQRKDHPWLQMEDMDLLKSAQLYQTDLVSGKSGITLAGILLLG
                     NDTTILTALPHHRTDLILRKFDLDRYDDRDDVRTNLIESYERIMAFVAKHLPDPFFLE
                     RDQRISLRGSIFREVASNILIHREYMNPFPAKLIIERGLVRTENSNRPHGFGPIDPTH
                     FSPMPKNPVIARFFRELGRADELGSGVRNLMKYGKAYGGKDPELVEGDIFRIKVHYPE
                     TETQVTPQVTPQVTPQVEKILG"
     assembly_gap    1389..1488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1493..2035)
                     /locus_tag="P5541_08355"
     CDS             complement(1493..2035)
                     /locus_tag="P5541_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRV72035.1"
                     /translation="MLDSFVTSKTRIKLLLKFFINPDTTAYLRGLADEFGESTNAVRV
                     ELNRLSRAGILDSRAEGRTILYNANKKHPLFPEIQRIVAKTVGLDRLVEQVVSRLGNV
                     ELAFVTGDYARGIDSGLIDLVLVGEVDEAYLVELAKKAEKLIDRKIRTLVLAREEYQK
                     LEEKFTKEKALVVWEKPEKA"
     gene            complement(2684..3676)
                     /locus_tag="P5541_08360"
     CDS             complement(2684..3676)
                     /locus_tag="P5541_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRV72036.1"
                     /translation="MPHLDMFSARDLEDLCRETSTKMKVAPIVVEKDYWTCWVLDRLF
                     SDPLVSEQIMFKGGTTLSKVFGLIERFSEDIDLILNWEMLKEKMGPIEAQRSKTGQDK
                     FNKESVEVSREYLKEEFHDSVRKALGEIVQTKIADDEPDVIEITYPRSFRDPYLRPGI
                     RLEIGPLAGWEPNDKFTITPYAAEAFPDLFSNPRITVRAISDKRTFWEKATILHQEAH
                     RPARKDQPIRYSRHYYDLMRMAVSSKRQNYLERVDLLEEVVSFKKKYYPCGWASYDSA
                     KPGSMKLVPPRERIRTLELDYQQMKTMIFGEAPEFGMILETLQDLEDSINVVPR"
     gene            complement(3663..4286)
                     /locus_tag="P5541_08365"
     CDS             complement(3663..4286)
                     /locus_tag="P5541_08365"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HRV72037.1"
                     /translation="MAENITRAIKDQINKKGRGWIFSPKDLSRLGSSEAVHVTLHRLE
                     KQGKIRRVARGLYDLPRYSNLLQSNLSPDIDQAAHALARKFGWRIIPNGNVILNMMGI
                     STQVPARYEYLSDGPYRKFRIGGTEISFRKVAVKEASFRCSESVLVVQGLRALGPNAI
                     DDRAIEKIRAWLPEGMRRKVLEDTRNVTKWIYDAIRKICREEHNAAS"
     gene            complement(4987..6432)
                     /locus_tag="P5541_08370"
     CDS             complement(4987..6432)
                     /locus_tag="P5541_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV72038.1"
                     /translation="MPITSSENILRVLSAFNPWWSTGSIPQHFRKGFKRFAWSEVQKY
                     LHHPDIRRAVVLTGARRVGKTTILYQTVDDLLQKRIDPRRILFVSLDHPMLKLSNMSE
                     ILGCFRQNIYGDDDVYYLFDEVQYADDWDRWIKTIYDTQGQARIVATGSTSPVLAEKA
                     AESGVGRWIVLKVPTLSFFEYCELLQAKERPVLPPDIRPTGLSGIDRREMSEIFVKLS
                     GLQKHFHRYLLVGGFPELVLSSDDYLAQQVMRDDVVDKVLKRDIPSLFNIRNVAELEK
                     IFIYLCLYSSNIISVDAISKELGGVSRTTVENYLRYLESANLIYRSDPVALTGKKILK
                     SRSKIYIADAAIRNAILMKSDILADSEEMGIMAETAVFKHLASFYYKNLTRVGYYRDG
                     QKGKEIDIVVDFPGGKILVEVKYRDDPAIAGNDAIKVMADRAVASIVVTKNENDYGIQ
                     PLPGGKSLVKIPAYAFLYLLGNAEKHGHQGA"
BASE COUNT         1592 a         1616 c         1718 g         1697 t
ORIGIN      
        1 atccccaaaa ttttttcgac ttggggggtg acttgggggg taacttgggg ggtgacttgg
       61 gtctcggttt ccgggtaatg cactttgatc cggaaaatat caccctcaac gagttcgggg
      121 tcctttcctc catatgcttt cccatatttc ataaggttcc ggacaccgga tccaagttcg
      181 tcggcccgcc ccagttccct gaaaaatctt gcgatcaccg gattcttggg cattggcgag
      241 aaatgagttg gatcgatcgg accaaaaccg tggggcctgt tgctgttttc agtacgaacc
      301 aggcctcgtt cgatgatcaa tttggcaggg aaaggattca tatactccct gtggatgaga
      361 atgtttgaag caacctcccg gaaaatcgag ccgcgaaggc tgattcgctg gtccctctca
      421 aggaaaaagg ggtctggaag atgtttggct acaaaggcca ttatcctctc gtagctttcg
      481 atcaggttgg ttcgcacatc gtcccggtca tcgtaccggt ccagatcgaa tttgcgaagg
      541 atcaggtctg tacgatggtg gggcaacgcc gtcagtatgg ttgtatcatt tcccaacaag
      601 aggatacccg cgagagttat tccactttta ccgcttacaa ggtccgtttg ataaagttgg
      661 gcgctcttga gaagatccat gtcttccatc tgaagccagg gatgatcttt tcgctgtatg
      721 ccagccatct ttctggcccg atctataaga tcggtgcgca ggtcggacat ttttgcatag
      781 gggaagatcc tgttttctga ataggttgac tgcttccgac gatacagttc tgctaccaga
      841 agtgtgttgt cggtgatgtc cagatctcca tcttcattcc gatcaaagat gcgcccgctg
      901 catctatgga cctgagaact ctcaggaaca tatattccaa ggatccgctt gccgcccaat
      961 tccaattcat ctacggacag ataacaggtc gggtggatct tttgagggtt gttgattgag
     1021 gttatgaaat ccttctttat gtgttccagt tttccagggt caatgcctag aatctctcca
     1081 gaatcacgga cgccgagcag cagggtgccg ccatttcggt ttaaaaaggc gcagacggtt
     1141 tcgtaaacat tcctgttaag accgttgcgg gattctttga attcaagggc caatccttcc
     1201 ccctgattaa tcagtttgcg aaggttttcc gtattttcct tcatgtaacc aacccctttt
     1261 tgaagggttc acgagttttt gtcgttcact gtttaccgtt atccgcttac gcagttgatc
     1321 ctgccttccc tgttgcatgc cttccaggtt ttatgttttg cattcccgtt caccgttgac
     1381 cgttgaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gtttacgcct
     1501 tttcaggttt ctcccatacc accaacgctt tttccttggt gaacttctcc tccagtttct
     1561 ggtactcctc gcgggcaagg accagggtgc ggatcttgcg gtcgatgagt ttttcggcct
     1621 tttttgccag ttccacaagg taggcttcgt cgacttcgcc gacgaggacg aggtcgatga
     1681 ggccggagtc gatgccacgg gcgtagtctc cggtgacgaa ggcgagctcc acgttcccca
     1741 ggcgcgagac gacctgttcg acaagcctgt cgaggcccac ggtcttggca acgatgcgct
     1801 ggatctcggg gaagaggggg tgctttttgt tggcgttgta gaggatggtg cggccttccg
     1861 ccctggagtc gaggatgccg gccctggaga ggcggttgag ctccacccgc acggcgtttg
     1921 tggactcgcc gaattcgtcg gccagacccc gcaggtatgc ggtggtgtcg gggttgatga
     1981 agaatttgag gagcagtttg atgcgtgttt ttgatgttac aaagctgtcg agcatgctac
     2041 ctccaggggc gtgagaagtt agacgtgaga cgggagacgt gaaagcacaa aaacgtgaga
     2101 cgcaataaca aaaacccaaa accagggctt ctcaccacag gcaaacccaa ttggggtcag
     2161 accttgattt taaccaattg cgctacaaaa acccaactgc gccaaccgtc aacggtaata
     2221 aggttcaaca caaagagtaa atcgaaaaat tcaaaagcgt aaacggtaaa cagtgaacgg
     2281 tgaacggtga acggtaaaaa caaccgcagg atcaaggcaa acccttctca ccacagagta
     2341 cgcagagggt ggggccgcag agaagggcaa gaaatatttt tgttaggatc aagtggaaat
     2401 ccaaaccttt ccaccgcaga ggacgagtta cgagttacaa ggtgccccaa gcggagcgcc
     2461 agggtgcgac ttacagaccc tattacgagt aattttattg ctcatggagg ataaaggcaa
     2521 gtgcgtaaac ggtaaactgt caacggtgaa cgggaaaagc cagagccaaa ccttttcatc
     2581 ccagacaagg tatcgttgat ggcaaaccgt cggatcagtt ccgaattgca gagaatgagt
     2641 tgaaaaaagc atgatcttca gggacaggaa gtattgacac accttagcga ggaaccacgt
     2701 tgatgctgtc ttcaaggtcc tgaagtgttt ccaatatcat gccaaattca ggggcctcgc
     2761 caaatatcat ggtcttcatc tgctggtaat ctaattccag ggttcttatc ctttccctgg
     2821 gaggtaccaa cttcatcgaa ccgggttttg ctgaatcgta cgaggcccac ccacagggat
     2881 agtatttctt tttgaaggaa accacttctt ccagcaggtc tactctttcc agatagttct
     2941 gtctttttga agagacagcc atccgcataa gatcatagta gtgcctggaa taacgtatgg
     3001 gctgatcttt gcgggcaggc ctatgggcct cctgatgaag gatcgttgct ttttcccaga
     3061 aggtcctctt atcacttatt gcccttacgg tgatacgagg attagaaaac aggtccggaa
     3121 atgcttcagc cgcgtaaggc gtgatggtga atttatcatt aggctcccaa ccggcaaggg
     3181 gaccaatttc caggcgtatc cctggcctca agtaggggtc cctaaaactt cgggggtagg
     3241 tgatctcgat cacgtcaggt tcatcatcag cgatctttgt ctgcactatc tccccaagag
     3301 ccttgcggac actatcgtga aactcttctt ttagatactc tcttgatact tcgacgcttt
     3361 ctttgttgaa cttgtcctgc ccggttttgc tgcgttgggc ttcgataggg cccatttttt
     3421 cttttagcat ctcccaattc agtatcagat cgatatcttc tgaaaaacgt tcgatcaggc
     3481 caaagacttt tgaaagggtg gtaccacctt tgaacatgat ctgttcactg acaaggggat
     3541 ctgagaaaag gcggtccagc acccagcatg tccagtagtc cttttcgaca acaatggggg
     3601 caaccttcat tttggtggaa gtttcccggc agaggtcttc tagatcccgg gcagagaaca
     3661 tgtcaagatg cggcattatg ttcctccctg cagatctttc ggatagcgtc gtagatccac
     3721 ttggtaacgt ttcgagtatc ctcaaggact tttctcctca tcccttccgg gagccatgcc
     3781 ctaatttttt ctatagccct gtcgtctata gcatttggac caagggccct cagtccctga
     3841 acgacaagaa cgctttcgga gcatctgaag gatgcttctt tcacggcaac ttttcggaaa
     3901 gaaatttccg tacccccgat ccggaatttc cggtatggac catcggaaag atattcgtac
     3961 ctggcgggga cctgggtgga tatccccatc atgttgagaa tgacgtttcc gttcgggatt
     4021 atccgccacc cgaatttcct ggccagggca tgggcagcct ggtcgatgtc agggctcagg
     4081 tttgactgga gaaggttact gtacctggga agatcgtaaa gtccacgagc cacccttcgg
     4141 attttgccct gtttttccag cctgtgaagg gtcacatgaa cggcttccga ggaaccaagc
     4201 cttgacaggt ctttgggaga aaagatccag cctctgccct ttttatttat ttgatctttt
     4261 attgccctgg ttatgttttc agccattttt gccactcctt ttgtaagtat tattatacaa
     4321 tttttattac atatggcaag agagagcagg agggcaagtt tgaagggtga aggaagacct
     4381 gaaacaagga ggcaggcgtc aggcagcagg cgacaggaaa ttcaaaacca aaaccttttt
     4441 tcaccgcagg ggacgcagac gcagcccctc attgaaacgc ttcgggaggc tttgctctgc
     4501 cctagctggc gtaccgccag cgtagggtag aagagctatt ttttaggtaa gccaaggcaa
     4561 agacctaaaa atatttgcac cacagacaat gcacctcgtt ggatcacccc cctcgttaat
     4621 gcacctccct cgttacagca cctcgggacc ttgtcgggac tatgtcggca ggcttcgctc
     4681 tgacctggcc ggcatgctgt cggcgtaggg tagggcgcaa agtaaaggca aaagcaaggc
     4741 ttcagtaggc gggaagagca ggatccgtat atcgtgaatc gtggattgtc attctgagga
     4801 atgaaatgac gaagcggaac gtccgcccac cggcgggaat ctggtcctgg tttcaatgca
     4861 aaaccagatc cctcgttttc gctcgggatg acaacagagg caaatgcaga tctccgccgg
     4921 tgagcgggat tcgagatggc aacaaagaca gggacctgaa cgtagaatcg gaccagatat
     4981 tgagtctcaa gcgccctgat gaccatgttt ttcggcattt cccagcagat aaagaaatgc
     5041 ataggccggg atcttcacaa gactcttgcc acccggcagg ggttggatgc cgtagtcgtt
     5101 ctcgttcttt gtcaccacga tggaggcaac agccctgtcg gccattacct tgatagcatc
     5161 attcccggct atggcaggat catcgcgata tttgacctcc acaagtattt tcccgcccgg
     5221 gaagtcgacc actatgtcga tctccttgcc tttttgcccg tcacgatagt atccgaccct
     5281 ggtaaggttt ttgtaataga aagaggccag atgtttgaat acggccgttt cggccatgat
     5341 ccccatttct tcggaatcgg caaggatgtc gcttttcatc agtatggcgt tgcgtatggc
     5401 ggcatcggcg atgtatatct tggacctgga tttcagtatc tttttccccg tcagtgccac
     5461 aggatcgctc ctgtagataa gattggcact ttcaaggtaa cgcaggtagt tttccacagt
     5521 tgttcggctt acgccaccaa gttctttgga tattgcatct accgagatga tattcgagga
     5581 atagaggcaa agatagatga agatcttctc cagctccgcc acattcctta tgttgaaaag
     5641 ggaggggatg tcacgcttga ggaccttgtc caccacatca tccctcatca cctgttgggc
     5701 aagatagtcg tcgctggaaa ggaccaattc agggaacccc ccgaccagga ggtagcggtg
     5761 aaagtgcttc tgcaggccgg aaagttttac gaagatctcc gacatttccc ttctgtcaat
     5821 tcctgacagc cctgtggggc ggatatcggg ggggagcacc gggcgctctt tagcctgcag
     5881 cagttcacag tattcaaaga aggagagggt tgggaccttt aggactatcc atcgaccaac
     5941 gccgctttcg gcggctttct ccgccagaac cgggctggtt gaaccggttg ccacgatccg
     6001 ggcctggccc tgtgtatcgt aaatggtttt tatccagcga tcccagtcgt ccgcgtactg
     6061 gacttcgtca aacaggtaat aaacatcgtc atccccgtaa atattctgac ggaaacagcc
     6121 aagtatttcg ctcatgttcg agagtttcag cattgggtga tccagggaga caaaaaggat
     6181 ccgccttggg tcgatccttt tctgcaacag atcgtcgacg gtctggtaaa gaattgtagt
     6241 tttcccgacc cttcgagcac ccgtaagcac tacagccctg cgtatatcgg ggtggtggag
     6301 gtatttctgg acctcggacc aggcaaagcg tttgaaccct ttgcggaaat gctggggaat
     6361 cgaacctgtg ctccaccagg ggttgaaagc ggacaggacc ctcaggatat tctctgagct
     6421 tgttattggc atagtcttca ccaccaaatg tctttattag ggccttaaaa tgcatatttt
     6481 gtgttattta tgtatatcat actttacact ttatccacgg gaaatgcaag gacctttaat
     6541 tcaactgcgt caacggtaaa cggaaaagcc caagacagaa aagcagacag tagggggcag
     6601 aaagaaaaac aaacctgaaa atgcgtgaga agtgagaggt tagatgtaaa cccggggaga
     6661 caggagtcag gcggcaggag gcaggaaagg caagatcaag aacgaggcga aggggcggaa
     6721 aca
//
LOCUS       DAOTIQ010000080         6635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_13716_length_6635_cov_7.867933, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000080 DAOTIQ010000000
VERSION     DAOTIQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13716_length_6635_cov_7.867933"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1537)
                     /locus_tag="P5541_08375"
     CDS             complement(293..1537)
                     /locus_tag="P5541_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRV72039.1"
                     /translation="MPIDRTRAMELHRKARGKIKIYPTISIHNEEDLALAYIPGSVPP
                     ALEIQQDEGMSYEYTGRGNRIAVITDGSAVLGLGNVGPHAALPVMEGKCLLFKLFGDV
                     NAIPVCLDTQDTDDIIHFCTLMAPTLGGINIEDVASPKTFTIVRELRSKINIPVLCDD
                     QHGTAVIILAGIWNAMEVQGKKLEDVKVVVNGAGAAGIATSELLLRAGARNVVVLNSS
                     GILNPDNQKMNHIQQELSEKTNPESIKGGLKEALEGAEVFIGLSKGGVFPAEYIKKMA
                     KGPVIMAMALPEPEIIPEEAWAAGAGIVATGLANCPNAMPNTLSSPGIMRGLLDVRAT
                     LLNHNMLLAAARALADVVDRRRLGPGKIMPDVFCDESAPRVAEAVGQAAIAEGYAAKE
                     VPKGEIYNNLWHTLYGNEMIRI"
     gene            complement(1572..2129)
                     /locus_tag="P5541_08380"
     CDS             complement(1572..2129)
                     /locus_tag="P5541_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRV72040.1"
                     /translation="MKPFRIEPARAQLLLIDLQEKMLPAVSSHETVSRNSIILLNACG
                     IMNVPVKYTEHYPRGLGSTVSSIADNFPKGSARTEKIHFSCCMEEGFDSFLRMEGRDQ
                     IIVAGVETHICVLGTVMDLLATDLRVAVAADGCSSRNPEHHRLACDAMASAGALVVPV
                     ETLVYQMLGRAGTTEFKAMLPFFKE"
     gene            2268..2918
                     /locus_tag="P5541_08385"
     CDS             2268..2918
                     /locus_tag="P5541_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72041.1"
                     /translation="MGKKDPGKDHEGENYDMSGDWTVGDILRAMSGRDCPDYVERMAS
                     QIREWMVAALSGENDLPAEDFLFGFGDRKQGDPEALLSQLEWVDDHADILPATTEPIG
                     HEDAVVLCLGPVDFDEGMRMAIDYAALFGRDHCKRVWMISDSWVIGEVVRYVQHFTAL
                     ASHGVELRFIMVTPWGWTEIPIALEEGEPGRLRWESRQKTGQNNSFRKNRHDESHS"
     gene            complement(2979..3176)
                     /gene="thiS"
                     /locus_tag="P5541_08390"
     CDS             complement(2979..3176)
                     /gene="thiS"
                     /locus_tag="P5541_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRV72042.1"
                     /translation="MITVNGEPMDWSDGMTIRDILDYKRYVFPLLSVWIDDVAVPRDK
                     FDTTRVPDGSKVQVIHMISGG"
     gene            complement(3181..5355)
                     /locus_tag="P5541_08395"
     CDS             complement(3181..5355)
                     /locus_tag="P5541_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV72043.1"
                     /translation="MEKMKVLAEWSYDPAKPFRGYTGKTLKVNVGDMSFESKDVSEDM
                     KHKFTGGRGFGLKLLWDSVKDTTKWNDPENEIVISGGPLCGVTQYPGMGKFYAVFVSP
                     LTNQTYDSNAGGHFGPLLKFAGWDAIEIQGKAKKDVVVVVDADAGKVQILESPFDDVN
                     AYTVTDALHEYYGDPNDINDRRFVSVVSSGKSAEHTFWGCLNSSFWDTHRKIPRLKQA
                     GRGGGGSILRDKKIVALVVKTHDLSGTANNPADVDTLTRLGAKLHKEIITLDSKQCRM
                     RTAGTAHLNEIMNEYHLLPTMNYKFGQHPDADKIHSKVYEKLYTQGISDGCWYGCSMA
                     CAKAVDGYELKTGPLKGTKVSVDGPEYETAASFGPNVGVYDPAWTIETNFYADHYGFD
                     TISAGTGIAFVCECFEMGLINKKHTNGLELKFGNKDDIMELIHRMAEGKDDFAVTVGQ
                     GIHRMKDLFAEKYGADKNIMERIGMEGQGIEVSEYVPKESVAQWGGFFLTLKGPQHDE
                     AWLIFMDRVNKQLPTFEDKAEALHYFPNFRTWFSLVGLCKLPWNDIEPADNATKYPPQ
                     EAARVPEHVQNYVEIFNAVTGQNITKEDLIHQAERVYNFQRVFQLRLGKGTRKFHNIP
                     DRALGPVWADEWEARTEYYDNELKAGGVDPAGLSSQEKCDKLQKIRREQWEVLVDTVY
                     KRRGWDKNGIPTVKKLKELGMDIPEVVAVVEKYAKPEDQWKD"
     gene            5537..5614
                     /locus_tag="P5541_08400"
     tRNA            5537..5614
                     /locus_tag="P5541_08400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5572..5574,aa:Pro,seq:cgg)
     gene            5764..6231
                     /gene="smpB"
                     /locus_tag="P5541_08405"
     CDS             5764..6231
                     /gene="smpB"
                     /locus_tag="P5541_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRV72044.1"
                     /translation="MDGSVVARNRKARHDYFILETIEAGIALTGTEIKSVRAGKVNLK
                     DGYARVQGSEVWLYNVHVSPYEQGTYYNHEPLRPRRLLLNAAEIRKLGSQTREKGLTI
                     VPLSMYIKQKRWAKVELALVKGKTGYDKRETIAERDAKRDIDKAMKEKRQNDF"
     gene            6243..6591
                     /gene="ssrA"
                     /locus_tag="P5541_08410"
     tmRNA           6243..6591
                     /gene="ssrA"
                     /locus_tag="P5541_08410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1429 a         1774 c         1803 g         1629 t
ORIGIN      
        1 ggtgttcctt tcggaccccc gtaacctgtc atgccaccgg gtaataggaa gtgacggaag
       61 catgacaggt tacgggggtc cgaaaggaac accacttaaa aaaagaatcc ttgcctacga
      121 gaaaaaatta cagaaatgat ccaggctagg taagccgtca aaaattgacg gcaaccggaa
      181 aatctaaacc ataatccagc gatgaaaaaa tggccggagc caaaaagctc cggccatttt
      241 tgttctggtt ttcctgactc cttgatgtct tacgccttct tgttttaatt ttctagatcc
      301 tgatcatctc gtttccgtag agcgtatgcc agagattgtt gtagatctcg cctttaggta
      361 cctcttttgc tgcataaccc tctgctatgg cagcctgtcc taccgcttca gcgacccttg
      421 gagctgattc gtcgcagaag acgtcgggca ttattttccc cgggccgagt cttcttctgt
      481 ctaccacatc cgccagagcc cttgcggctg cgaggagcat gttatgatta agcagtgtgg
      541 ctcgcacatc cagaagacct ctcatgatac ccggagaaga aagggtgttc ggcatggcgt
      601 tggggcagtt ggcaaggcca gtcgcgacga tcccggcccc tgccgcccag gcttcctccg
      661 ggatgatttc aggttcgggc aatgccatcg ccattatcac gggacccttt gccatttttt
      721 taatgtattc cgccgggaat actcctccct ttgaaaggcc gatgaaaact tccgctccct
      781 ccagggcttc cttgaggcca cccttgatac tttcagggtt tgttttttcg gaaagttcct
      841 gctgaatgtg attcattttc tggttatcgg ggttcaggat acccgaactg ttaagaacga
      901 cgacgttcct ggcccccgcc cggaggagaa gctcggatgt cgctattccc gctgcccccg
      961 ctccgttgac gacaaccttg acatcctcaa gtttcttacc ctggacttcc atggcgttcc
     1021 atatgcctgc aagaataatg accgcggtgc cgtgctgatc gtcgcaaaga acggggatgt
     1081 tgatcttgct ccggagttcc ctgactatgg taaatgtttt ggggcttgcc acgtcctcta
     1141 tgtttatgcc ccccagagtg ggggccatga gagtgcagaa atgaataata tcatctgtgt
     1201 cctgcgtatc caggcagacc ggtatggcgt ttacgtcacc gaacagtttg aacagaagac
     1261 atttgccctc catgacgggg agggctgcgt gaggcccgac gttccccaga cccagaacgg
     1321 cgcttccatc cgttattacg gctatcctgt tgcccctgcc cgtatactcg taactcatcc
     1381 cctcgtcttg ctggatctca agagctggag gtacgcttcc agggatgtag gccagggcaa
     1441 ggtcctcttc gttgtggata ctgatggttg ggtaaatctt gatcttcccc cgggctttcc
     1501 gatgaagttc cattgctctt gttctgtcta tcggcataat attacctccc agatccggcc
     1561 cgtgtggccg ttcattcctt gaaaaacgga agcatggcct tgaactctgt ggtccctgcc
     1621 ctgcccaaca tctggtatac cagtgtttcg acggggacca caagtgcccc tgccgaggcc
     1681 atggcgtcgc atgccagcct gtggtgttca gggttccggc tggagcaacc atcggcagcc
     1741 acggccaccc tgagatctgt agcaaggagg tccattactg tcccaaggac gcaaatatgg
     1801 gtctcgaccc cggcaacgat gatctggtct ctgccctcca tcctcaggaa actatcaaat
     1861 ccttcttcca tgcagcatga gaaatggatc ttttctgtcc tggcacttcc tttcgggaaa
     1921 ttatcagcaa tactgctgac ggttgaaccc agtccgcggg ggtagtgctc agtgtattta
     1981 accgggacat tcatgatccc gcacgcattg agcaggatga tcgagtttct ggaaacagtt
     2041 tcgtgggagg agaccgctgg cagcattttt tcctggagat ctatcaggag gagctgtgcg
     2101 cgggcaggtt cgatcctgaa aggtttcatg cgaaagcacc ccgtttcctt tagttattcg
     2161 agctttgagg ttattatagg gcaaacagtg ctgttcatgt tccgtgtaga aacgggaaaa
     2221 atgtgttatg ctctgaaaaa atagagagcc ccggggaggt gtgtttcatg ggcaaaaagg
     2281 atccgggcaa ggatcacgaa ggagagaact acgatatgag cggagattgg accgtagggg
     2341 acattcttcg agccatgtcc ggaagggact gtcccgatta tgtggagaga atggcgtccc
     2401 agatccggga gtggatggtt gcagcgctgt ccggagaaaa cgatcttccc gcggaagatt
     2461 ttcttttcgg tttcggggac agaaagcagg gagaccctga ggcactcctc tcccagctgg
     2521 agtgggtgga tgatcacgct gatattcttc cggcgactac ggagcccata ggacacgaag
     2581 atgccgttgt cctttgcctt gggccggttg atttcgacga ggggatgcgt atggctatcg
     2641 attacgcggc ccttttcgga agggaccact gcaagagggt ctggatgatc agcgacagct
     2701 gggtcatcgg cgaggtggtc cgttacgtcc agcatttcac tgccctcgca tcgcacggcg
     2761 tcgagctgag gttcatcatg gtcacaccct ggggatggac ggagatacct atcgccctgg
     2821 aggagggcga acccgggagg cttcgctggg aatccaggca gaaaacaggc cagaacaact
     2881 cattcagaaa aaaccgtcac gacgaatctc actcctgaca tccttaacgg caaaaaaaag
     2941 gggccggtcc ttcaggaccg gccccttttt gcgtgttctc agcctccgct gatcatgtgg
     3001 atcacctgaa ctttcgagcc atcgggaacc cgggtcgtgt cgaacttgtc cctgggtaca
     3061 gcaacatcgt ctatccatac ggacagaagc gggaaaacgt accttttgta atccaggata
     3121 tcccggatcg tcattccgtc actccagtcc attggctccc cgttgaccgt tatcatcggt
     3181 tcagtccttc cactgatcct ccggcttagc atatttttcg acaacggcta ctacctcggg
     3241 aatatccatc ccgagttcct tcagtttctt tacggtgggt atgccgttct tgtcccaacc
     3301 gcggcgtttg taaacagtat cgacaaggac ttcccactgc tccctgcgga tcttctgaag
     3361 tttgtcgcac ttctcctggg aggaaagccc tgcgggatcc actccgcctg ctttcagttc
     3421 gttgtcgtag tactcggtcc ttgcttccca ctcatcggcc cagaccggtc caagtgccct
     3481 gtcggggatg ttgtggaatt tcctcgtacc ttttccaagc ctcaactgga agaccctctg
     3541 gaagttgtag acgcgctcgg cctggtggat caggtcttct tttgtgatgt tctggccggt
     3601 gacggcgttg aatatttcca cgtagttctg gacgtgctca ggtaccctgg cagcttcctg
     3661 agggggatac ttggtggcgt tgtcggccgg ttcgatatcg ttccagggca gtttgcacag
     3721 acccaccagc gagaaccagg tcctgaagtt ggggaagtag tggagtgcct ctgccttgtc
     3781 ctcgaaggtt ggaagctgct tgttgaccct gtccatgaag atcagccatg cctcgtcgtg
     3841 ctgcgggccc ttaagggtga ggaaaaatcc gccccactgt gcgacagatt ccttcggcac
     3901 gtactcggag acctcgatac cctggccctc cataccgatt ctctccatga tatttttgtc
     3961 ggcgccgtac ttctcggcga agaggtcctt cattcggtgg ataccctggc cgacggttac
     4021 ggcaaaatca tccttgccct cggccatacg gtgaataagc tccatgatat cgtccttgtt
     4081 gccgaatttg agttcaagac cgttggtatg cttcttgttg atcagcccca tttcgaagca
     4141 ctcgcagaca aaagcaatgc ctgtacctgc ggagatagta tcaaaaccat agtggtcagc
     4201 gtagaagttt gtctctatcg tccatgccgg gtcatagacg ccgacattgg ggccgaaact
     4261 tgccgctgtc tcgtactccg ggccgtcgac tgagactttt gttcccttca ggggaccagt
     4321 tttaagttcg tagccgtcga ctgcctttgc gcatgccatg gagcagccgt accagcatcc
     4381 gtctgaaatc ccctgggtat aaagtttttc gtagactttg gagtggattt tgtcggcgtc
     4441 ggggtgctgg ccaaacttgt agttcattgt tggaagaagg tggtactcgt tcatgatctc
     4501 attgagatgg gctgttccgg ctgtacgcat tctgcactgc ttggaatcga gggtgatgat
     4561 ctccttgtgc agttttgccc ccagccttgt gagggtgtcg acatcggcag ggttgttcgc
     4621 ggtgcctgaa agatcgtgtg ttttcacgac cagggcaacg atcttcttgt ctctgagtat
     4681 ggagccgccg ccgcctctac ctgcctgctt gagtctcggg atcttgcggt gggtatccca
     4741 gaaactgctg ttcaggcagc cccagaaagt gtgttccgcg gatttgccgg aagatactac
     4801 agatacgaac cggcggtcat tgatgtcgtt ggggtcaccg taatattcat gaagagcgtc
     4861 ggtcaccgtg taggcgttta catcatcgaa gggagactcc aggatctgga ccttcccggc
     4921 gtctgcgtca acgaccacga cgacatcctt ctttgccttg ccctggatct ctatggcgtc
     4981 ccagcccgca aacttgagca gtgggccgaa gtgtccaccc gcgttgctgt cataggtctg
     5041 atttgtgaga ggagagacaa acacggcata aaacttcccc attcccgggt actgggtcac
     5101 tccgcagagc ggcccgccgg agatgacgat ctcgttctcg ggatcgttcc actttgtggt
     5161 gtccttgacg gaatcccata acagtttaag cccgaatccc ctgcctccgg tgaacttgtg
     5221 cttcatgtcc tcggatacat cttttgattc gaaggacatg tcacccacgt tgaccttcag
     5281 ggtcttgccg gtatagcccc tgaaaggttt tgccgggtcg taggaccatt cggcaagaac
     5341 tttcattttc tccattacct gttccccccc tgaaaaagtt tgctagaaca cgataaatcc
     5401 tgctgcagat actgggattt ctccattgta cgcagaatat aggatccgat atcaatatta
     5461 tcatgattgt gcctggagta tcaatctctt ttatcgttga cggcctgctc cgtaaagtgc
     5521 tacaatgccc gtcgttcggg gcgtagcgca gcctggttag cgcgcctgct tcgggagtag
     5581 gaggtcggag gttcaaatcc tctcgccccg accagcaaga ggtgacggga tcccgtttta
     5641 atgcggggtc ccttttttgt agcgccggca agatctggcg gtttgagcct gacaaaaccg
     5701 gagtctgacg aaagttgact aataagaaca tcggtcctat aatgaccgtt gcggaggaga
     5761 agcatggacg gttctgttgt agcgagaaac cgcaaggccc ggcatgacta ttttatactc
     5821 gagacgatcg aggccgggat agcccttacc gggacggaga tcaaatccgt ccgcgcgggg
     5881 aaagtcaatc tgaaagacgg ttacgccagg gttcagggtt ccgaggtatg gctctataac
     5941 gtccatgtat ctccctacga gcagggaaca tattacaacc acgagccgct caggcccaga
     6001 aggcttctgc tcaacgcggc ggagatacgg aaactgggat cccagacgag ggagaaaggt
     6061 cttaccatag ttcctctctc catgtacatc aagcagaaaa gatgggcaaa ggtcgaactg
     6121 gccctcgtca agggaaagac cggctacgac aagcgggaga ccatagcgga gcgcgacgca
     6181 aaaagggaca tcgacaaggc gatgaaggaa aaaaggcaaa atgatttttg acaaatgaat
     6241 atgggggcga caggtctcga cggcaggatg gagggttccg ggcagcgtgc cgagttgccg
     6301 aaactcgaaa aacacggcgc aaacaataat tgccaacgat aactacgcac tggccgctta
     6361 agtagcgacc cgttcctcgg ggtttccgtc gccaggatcc cgatgtgaac gtcacaaatg
     6421 gtgactgctc ctcgcgcttc gttctcgcgc gtgaggtcaa gcctcagaga actcgggtaa
     6481 gggtatcccg tctccgggag acccgagccg acactaaagc ggagactgcg cacggagaaa
     6541 cccgcagctg gcccctgtcg gaccggggtt cgattccccg cgcctccacc atttgaaaaa
     6601 agacaacccg tctctccggg gtcattctcc ggggt
//
LOCUS       DAOTIQ010000081         6595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_13815_length_6595_cov_6.512385, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000081 DAOTIQ010000000
VERSION     DAOTIQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_6595_cov_6.512385"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="P5541_08415"
     CDS             complement(<1..400)
                     /locus_tag="P5541_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72045.1"
                     /translation="MSDKTYIKKMGHRFLFEEGIWTASGFLRDGNGNTMLFSGKTVIS
                     HEEDHWINSGFMEIEGEAPVRIENNYLIEPFPRGSDSTTWKSDNSDLGTLVGTFAVVG
                     DSILSAFRNLDGSIYGTEYLKNVDPDTYANR"
     gene            complement(446..1171)
                     /locus_tag="P5541_08420"
     CDS             complement(446..1171)
                     /locus_tag="P5541_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV72046.1"
                     /translation="MKIKIAQEVFQKYPDYRRIVVIGRDLDNAEEDTALLALLKEAEA
                     EVRQDPDLENFKEYPLIASWRKVFEDMGLNPNKFPPSIANLIKRTCAGKDLPFINRLV
                     AIFNIISLKYRIPCGGDDLGCVTGDVRLGPANGDEVFAHLGNPEAVENPAPGEIILHD
                     TGNGSVFCRGWCWKNGDPSKITPQTRFVAINLDAMPPVDGAVHRQAADELVDLVEKHC
                     GGKTSMKMLEPESPCLELQTGAN"
     gene            1245..2201
                     /locus_tag="P5541_08425"
     CDS             1245..2201
                     /locus_tag="P5541_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRV72047.1"
                     /translation="MDGQQQGRPRKALYFFCVGKGYDHVSRLAFERVREIFSPRELGI
                     EVDGYPVMEHIDGHGNSFCFMRQDMLVSYDFRRHLPVLREHFLDFDLAAEVNWHEGAN
                     APDKVLTVHTIGDVDAGIFPPADARFMRNLLHSLEKNRSDARLDDFTVVTEATHWTGT
                     FRDQNPEDLMQFPIPMLDIEIGSTLASWENAEAVEVLARSLASPFSSEEPLKNVICVG
                     GVHFEHSFSEAAMNVDHPFGISHILPNQWIVSGGYGSEDGYAKLRKAAQSIRGGVRAV
                     VYHEGIKSAYRDQCRRLGEELGVPVLKHKALRRPADLEWLKK"
     gene            complement(2224..3420)
                     /locus_tag="P5541_08430"
     CDS             complement(2224..3420)
                     /locus_tag="P5541_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV72048.1"
                     /translation="MASLTELLELHRLAKNEGGRYEKHRPLLQDLLGSGGKHFIGIAG
                     PRGAGKTVLLRQYAHKNENAFYLSLDTNKDGDLFELAKKLNMDLGFTHLLLDEVHCCK
                     EYDELLKRIYDFLDVRVIFASSVSLSIFRSSYDLSRRISIRQLYPFSFREFIFFKTGE
                     HLSELSIEDIVKKRGLSVPLRHANLFDEYLKGGVLPFALDEPDPLPLLKDIVKKVISG
                     DIPGIAKIQVDEIELLEKTLGFIGRSEVDGINYSSISRNVGVTKYKAAQYMELLEKAF
                     IIHQVFPRGTNVLKEPKILMALPYRLLYRDMEDCIGPLREDFFAGMMRAAGIPFHYLK
                     TRRGAKTPDYLIPNDNGDLVIEIGGRGKGPSQFKGIEAAEKLIFYPSANLDGMSRPLE
                     SLGFLC"
     gene            complement(3622..4914)
                     /locus_tag="P5541_08435"
     CDS             complement(3622..4914)
                     /locus_tag="P5541_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV72049.1"
                     /translation="MLETIRSLILDFQETLPETGVPRHLSIEAVRGKAAVCIGVRRSG
                     KSTYMFQVIKHLTESGIPMENILYLNFFDDRLHSLRQDNLGLVTEAYYSIFPEKKNTE
                     KIYCFFDEIQAVPHWESFVDRLMRTEKCEVYLTGSSARMLSREIATQMRGRALAWEMF
                     PFSFAEFLDYKGMDRSGPFPTRKRLLVQKAFDEYWDTGGFPEVAGLGPDLRIKIHQEY
                     FNTILFRDLIERHDISHPKALKDLAHRLVDNIGSLYSINGLTGFLKSLGYKAPKSVVG
                     DYLDWFEDAYFLFTVRIFDASFTRSNANPKKIYCVDHAMVSSVSSGILVNSGHLLENL
                     VFTALRRVYPEIFYYRTKTGREVDFIVPLRGKEQMLVQVSESLAEQSTRKRETDALAE
                     AMAELGVNSGIIVTRYEEGQIAGGNIKITPAWKFLLDL"
     gene            5433..5966
                     /locus_tag="P5541_08440"
     CDS             5433..5966
                     /locus_tag="P5541_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72050.1"
                     /translation="MKKRVSVVLATLLVALFVILSGEASAELWVDTFLTDFWTQQNIG
                     PQLSGIQPGGTTGQVLTKIDGTDYFTEWTTLYDWEPRVGSLENTVADHGRRLSDLEET
                     QYVFGLNLRLFDSRKVSWNVFANYSANRSKVDRCGINLTLKLGKSYEEKLIEKLGREN
                     AEMRAELEEIKNKLGIQ"
     gene            6163..>6595
                     /locus_tag="P5541_08445"
     CDS             6163..>6595
                     /locus_tag="P5541_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72051.1"
                     /translation="MKKRVSVIFATLLVALFVILSGEASAEDGLWVFTSDTATGAASV
                     FDISDISPQIGAIRYGGKAGQVLTKTNDEDFWTDWMDVYDWSPRVGALESAVAGHDRR
                     ISDLEETQYVFGLNMRVFDSRKMSWNVFANYSANRSKLDRVG"
BASE COUNT         1619 a         1661 c         1710 g         1605 t
ORIGIN      
        1 ccctgttcgc gtatgtgtcg gggtcgacgt tcttgagata ttccgttccg tatatcgagc
       61 cgtccaggtt acggaaggcg gagaggatgg agtccccaac aacggcgaag gtcccaacca
      121 gggtcccaag atcggagttg tcggatttcc atgtggtgga atcgctcccc cgggggaagg
      181 gttctataag gtaattgttc tctattctta cgggagcctc cccctcgatc tccatgaacc
      241 ccgagttgat ccagtggtcc tcctcgtggg aaataacggt tttaccagaa aaaagcatgg
      301 tgttaccgtt accgtccctc aggaacccgg aggccgtcca tataccctct tcgaagagaa
      361 aacggtgacc cattttcttg atgtaagttt tgtccgacaa aaaagttcct ccgtcccggt
      421 tattttcgtg atttgcctta tggtctcagt tggctcctgt ctgcagctcc aggcaggggc
      481 tttcaggctc cagcatcttc atcgaggttt tccccccgca atgtttttct acaagatcca
      541 ccagttcgtc cgcggcctgc ctgtgaaccg ccccgtccac ggggggcatg gcgtccaggt
      601 tgatggcgac gaaccgcgtc tgcggagtta tcttgctggg gtcgccgttc ttccagcacc
      661 agccgcggca gaagacgctt ccgtttccgg tgtcgtgcag gatgatctcc cccggcgcag
      721 ggttctccac ggcctccggg ttgccaaggt gggcaaagac ctcgtcgcca tttgcagggc
      781 ccaggcggac atcgcctgta acgcacccaa ggtcgtcccc tccgcagggg atccgatact
      841 tcaggctgat gatgttgaat atcgccacga gcctgttgat gaatgggagg tccttccccg
      901 cacaggtacg cttgataagg ttcgcgatcg aaggagggaa cttgttgggg ttcagcccca
      961 tatcctcgaa gaccttccgc cacgaagcga tcagcgggta ctccttgaag ttctccaggt
     1021 cagggtcctg ccggacttca gcctcggcct cctttagaag ggccaggaga gcggtgtctt
     1081 cctccgcatt gtccaggtcc ctcccgatca cgacgatgcg ccggtaatcc gggtactttt
     1141 gaaaaacttc ctgtgcgatc ttgatcttca acgatgaaca tctcctttcg cggatcctga
     1201 cgacatatat aataacagga caacattcag ggagggcatt tgccatggac ggacagcagc
     1261 agggcagacc ccggaaggct ctttatttct tctgcgtagg gaaagggtac gatcacgtct
     1321 cgcggctggc cttcgagagg gtccgggaga tattcagccc ccgcgagctt ggcatagaag
     1381 tcgacggcta tcctgtaatg gagcacatcg acggacacgg gaacagcttc tgcttcatgc
     1441 ggcaggatat gcttgtcagc tacgacttcc ggcgacatct tccggttttg agggagcatt
     1501 tcctcgattt cgaccttgcc gcggaggtca actggcacga gggggccaac gcgccggaca
     1561 aggtgctgac ggtccacaca ataggggatg tcgacgccgg aatattccct cccgccgatg
     1621 cccgcttcat gcggaacctt cttcattccc tcgaaaagaa ccgcagcgat gccaggctgg
     1681 atgactttac ggtcgttacc gaggccaccc actggaccgg gacattcagg gaccaaaacc
     1741 ccgaagacct tatgcagttc cctatcccga tgctggatat agagataggc agtaccctgg
     1801 caagctggga aaacgcggaa gctgtagaag tccttgcgcg aagcctggca agtccttttt
     1861 caagtgagga acctcttaag aacgtcatct gcgtcggcgg tgtgcacttc gagcactcct
     1921 tctccgaagc ggcgatgaac gtcgatcatc cgttcggcat aagccatata ctgcccaacc
     1981 agtggatagt atctggaggc tacggatcgg aagacggtta cgcaaaactc cggaaagctg
     2041 cccagagcat ccgtggaggc gtccgtgcgg tcgtctatca cgaggggata aagtctgcct
     2101 accgcgacca gtgccgcaga ctcggcgagg agcttggtgt ccccgttctc aagcacaaag
     2161 cgctccgcag gcccgccgac ctcgaatggt tgaaaaaata agggattttg tccgatattt
     2221 acttcagcag aggaagccca ggctttccag tggccggctc attccatcca ggttcgcgga
     2281 ggggtagaat atcagctttt ctgcggcttc gatgcccttg aactggctgg gtccctttcc
     2341 tctgcccccg atctcgataa ccagatctcc attgtcgttc gggatcaggt agtccggtgt
     2401 ttttgcgccc cgtctcgttt tgaggtagtg gaatggtatc ccggctgctc tcatcatccc
     2461 ggcaaaaaaa tcctccctca aagggccgat gcaatcctcc atatcccggt acagcaggcg
     2521 gtagggcaga gccatcaata tcttcggctc cttcagtacg ttggtgcccc tgggaaatac
     2581 ctggtggatt ataaaggcct tttcaagaag ctccatgtac tgggctgcct tgtattttgt
     2641 tacccccacg tttcgtgaaa tgctcgagta attaattccg tccacttccg atcggcctat
     2701 gaaaccgagt gttttctcaa gcagttcgat ctcgtccacc tgtattttgg cgatccccgg
     2761 aatatcccct gaaatgactt tctttacgat gtccttcaga aggggcaggg gatccggttc
     2821 atcgagggca aaggggagca cgccgccttt gaggtattca tcaaagagat ttgcgtgtcg
     2881 cagaggaacg gatagcccgc gtttttttac tatgtcttca attgagagtt cggagaggtg
     2941 ttctcccgtt ttaaaaaaga taaattcgcg gaaagaaaac ggatagagct gtcttatgct
     3001 gatccttctg gaaagatcgt aggatgatct gaatatggaa agtgagacgg aactggcgaa
     3061 aatgactctg acatcgagaa aatcgtagat ccttttgagc agctcgtcat actccttgca
     3121 gcagtggacc tcgtccagca gaagatgagt aaagccgaga tccatgttca gttttttcgc
     3181 cagttcaaac agatcaccgt ctttgttagt atcgagggag agataaaagg cgttttcgtt
     3241 tttgtgggca tactgccgga gcagcaccgt cttgccggct cccctgggcc cggctattcc
     3301 tatgaagtgc ttcccgcctg atccaagcag gtcctggagc agaggtctgt gcttttcgta
     3361 cctgcccccc tcgttctttg caagccgatg cagttcaaga agctccgtca gcgacgccac
     3421 gcgatcaccc ctcacgacat tccgcacaat gattttacag aatgatagac attttgtaaa
     3481 atcatttgac aagatgcacg tattaaggca tgcgatcttc ctccgttcat cgtttttacc
     3541 gatattgaaa caacccatag ggtcagggct tgaacctaac cttatgtata catgtaggca
     3601 ggtccgaagg ctttgtgcta tttagaggtc cagcaaaaac ttccatgcgg gagtgatctt
     3661 tatgtttccg cctgcgattt ggccttcctc gtaacgggtt actatgatgc cgctgtttac
     3721 tcccagttca gccattgctt cggcgagagc atcggtctct cttttgcgtg ttgactgctc
     3781 ggccagggat tcggaaacct gtacaagcat ttgttccttg cctcgcaagg ggacgataaa
     3841 atcaacctca cggccggttt tggtcctata gtaaaaaatt tccggataaa cccttcgaag
     3901 tgccgtaaaa acgaggtttt ccagaagatg ccccgagttg accagaatcc cggatgaaac
     3961 ggacgatacc atggcgtgat ctacgcagta gattttcttg gggttggcgt tgctgcgcgt
     4021 aaacgatgcg tcgaaaatcc tgacggtaaa gagaaaatac gcatcctcga accaatccag
     4081 gtagtcgcca acgaccgatt taggagcttt atatccaagc gacttgagaa aacccgtaag
     4141 gccgttgatt gaataaagag agcctatgtt gtccaccagc cgatgggcaa gatccttcag
     4201 cgctttcgga tgtgaaatat cgtggcgttc tatgagatcc cgaaaaagga tcgtgttgaa
     4261 gtactcctgg tggattttga tccgcaagtc tggtccaagt ccggcaacct cgggaaaccc
     4321 gcccgtatcc caatattcat caaaggcttt ttgaacaaga agacgttttc tggttggaaa
     4381 cggacctgac ctgtccatcc ccttgtagtc cagaaattcg gcgaacgaga aaggaaacat
     4441 ctcccatgca agagcccgtc cccgcatttg agtggctatc tctcttgaaa gcatcctggc
     4501 ggaagaaccc gtaagataga cttcgcactt ttccgtgcgc atcaggcggt ctacgaacga
     4561 ttcccaatgg ggaaccgcct gtatctcgtc gaaaaaacag tagatctttt cggtgttctt
     4621 tttttccggg aagatcgaat aatatgcctc ggtgacaaga cccagattgt cctgacggag
     4681 agaatgaagg cggtcatcga aaaagttcag gtagaggatg ttttccatgg gaattccact
     4741 ctcggtgaga tgctttataa cctgaaacat ataggttgat tttccgcttc gccttacccc
     4801 tatgcatact gcggcttttc cgcgaacagc ctcgatgcta agatgccggg gtactcccgt
     4861 ttcaggcaat gtttcctgaa aatccagaat gagggatctt atagtctcaa gcatcttgtc
     4921 accccaccgg taaaacttat tgccaatatt ggctcagtct ggtaataagt attaccgatt
     4981 atgcgatcag tgtcaacagg gttgcggctt ttgtcgtgcg gttatccctc cgtgacattc
     5041 cgtaaaatta ttttccagaa tgataggcat tttgtaaaat cattggacaa gatgcaagat
     5101 tgataaaaat gattacagaa acccgtagcc ctccatggaa aaatcctgtt aacttatagt
     5161 cccatttgac gacccccccc ccgtgtttta atacaaaagc aagcactcaa catatcctat
     5221 cgttaatcct gtaccccatt tattccctcc accaaaaagt gctgcagaag gtctccattc
     5281 acggaaagaa aaaaagaaga gggagatgtt gggacgggtt tgaaaaaccc gctctggcag
     5341 aagggttttg gaaagacaga gtttcaccac ggataccccg taagggggca actcgaaaaa
     5401 accctctata agggaaaagg aaggagataa caatgaagaa gagagttagc gttgttcttg
     5461 caacgctcct ggtcgcactt ttcgttatac tttccggaga agcaagtgcg gaattatggg
     5521 tggatacatt tctaactgat ttttggacac agcaaaacat cggaccccag ctctcaggca
     5581 ttcaaccagg gggtacaact ggtcaagttt taacaaagat cgatggaaca gactatttca
     5641 cggagtggac cactctttac gactgggaac cacgagtggg atcacttgag aatactgtcg
     5701 ctgatcatgg cagaaggcta agcgatctcg aagaaacgca atatgtcttc gggctgaatc
     5761 tccggctttt tgattcaaga aaagtgagtt ggaacgtctt cgcgaactac tcggcaaacc
     5821 gctcgaaggt ggaccggtgc ggaataaatc ttaccctgaa gctcggcaaa tcatacgagg
     5881 aaaaattgat tgaaaaactt ggcagggaaa acgctgagat gcgtgccgaa cttgaggaga
     5941 ttaaaaacaa actcggaatc caatagtgcc tggtgctttc cttttgggat ccccattccc
     6001 ggaaaaaaaa gaagagggag atgtcgggac gggtttgaaa aacccgcgct ggcagaaggg
     6061 ttttggaaag gcagagttta accacggata ccccgacacg gataccccgt aaggggggca
     6121 actcgaaata accctctata agggaaaagg aaggagaaaa caatgaagaa gagagtgagc
     6181 gtaatttttg caacgctcct ggtcgcactt ttcgttatac tttccggaga agcaagcgcg
     6241 gaagatgggt tatgggtctt tactagcgac acggcaacag gggcagcaag tgtttttgat
     6301 atatctgata tatctcctca aattggagca atccgatatg gaggaaaagc aggacaggta
     6361 ctgacgaaga caaatgacga agatttctgg actgattgga tggatgttta cgattggtct
     6421 cctcgcgtgg gagctcttga gtcagctgtt gctggccacg acagaagaat aagcgatctc
     6481 gaggagacgc agtacgtctt cgggctgaac atgagggttt ttgattcccg aaaaatgagt
     6541 tggaacgtct tcgcgaacta ctccgccaac cgctccaaac tggaccgggt cggag
//
LOCUS       DAOTIQ010000082         6445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_14264_length_6445_cov_7.726291, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000082 DAOTIQ010000000
VERSION     DAOTIQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_6445_cov_7.726291"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..812
                     /locus_tag="P5541_08450"
     CDS             159..812
                     /locus_tag="P5541_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRV72052.1"
                     /translation="MIAMDTALTGTSLLIYGMVLGISIAAPVGPIGVLCIRRTLAAGL
                     LPGLATGLGAAVADGLYGAVAAFGIASVANLLMAVNLWLRLAGGTFLLWLGLRTVSRK
                     PEDPLQEIPQDPGGFSLATDFASTFVLTLANPMTIISFSAIFMGLGKDIATAGATGPL
                     LLVMGVFSGSALWWLGLSSFFSLFRKKMTPTAMKWVNRVSGSVIVLFGAIVLLDAII"
     gene            920..1564
                     /locus_tag="P5541_08455"
     CDS             920..1564
                     /locus_tag="P5541_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72053.1"
                     /translation="MKGKFLLMVLVALLLFPLPCALAAECPPEKPDAEPLFETEGIFV
                     GKLDGNLVIFLDNRDGDRQVSFTLDREQASRLFSYAVEARNAAPGMNPFAPTGNMEVP
                     KKQRPMEPLMLETPEGSALWAHVAGRDYRNSSFFADFVLAPSDSGGSPDRNYMQAMTI
                     YETAPTFPKEEIIDKATGQPIGEPAQPEAKWGDEFSAFLSALQKFTGSKLLIIG"
     gene            1561..2271
                     /locus_tag="P5541_08460"
     CDS             1561..2271
                     /locus_tag="P5541_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRV72054.1"
                     /translation="MTSGRRGFTLVELLAVLMIVGILAGTMLLVMGSGTEKARLAVCQ
                     RDGRTVGSAARIYVWENPGSEPELQDLIDSGLIERAPNCIGEGTCSWSPEELRLVCGS
                     QEPAEPEPLTPLGSTFAEISGSMAARVLQYYQDNGTPLSTWQDERYAAIGLEKTFWDN
                     PVEGIMYVPQGLFVKIRPAVPGITFQVTTTGGETKTLTSDLNWNIWHNVVTDTWYFHS
                     SNDSDNAFDPQTLVIIRD"
     gene            complement(2276..3154)
                     /locus_tag="P5541_08465"
     CDS             complement(2276..3154)
                     /locus_tag="P5541_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV72055.1"
                     /translation="MSPSVGRFTPFFVLLLGIIGISTGSIFARYADASPIVISFYRSG
                     IAAAVMAPLVFVRHRQELFGLQRKDLLRVVLAGTFLALHFSTWITSLFYTSIASSVVI
                     VETIPVWTAILSPFVSGDRVSRVTWAGIALSFCGVLVISAGDFALGGRALWGDILALF
                     GAWFATLYFLTGRVVRAKVSLPVYTFVCYGAAAFVLLVVIIAAGLPLKGFSTSTWTAF
                     IGMALVSQILGHSSYNWALRYLSASLVAVALLGEPIGATLLAWILFGETLTLAKIIGG
                     TLILLGIVVAVRGERT"
     gene            3287..4399
                     /locus_tag="P5541_08470"
     CDS             3287..4399
                     /locus_tag="P5541_08470"
                     /inference="COORDINATES: protein motif:HMM:NF028293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HRV72056.1"
                     /translation="MSRIGPFDFSLREFGGSLGDLGTLLPLAVGYITVCGVDPAGMLV
                     FLGLANIITGVIYRLPMPIEPMKVLAVMAIAQAWSPGQIYASAFAMGITWLFLGLSGA
                     INWIAAHTPHSVIRGIQFSLGILLAIQGFRMIQGAWFLAAVSILIILLLRDNRFAPAS
                     LVLVAGGILLMAFRGDLSSIQPMSLALPQLLKLDMSLLWPSMRDGGFSQIPLTATNAV
                     IATSALITKYWQDRSVSVRQLSLNMGVMNLLGPFIGGIPLCHGAGGLAGQYYFGARTG
                     GANIIEGSLEIFLGIFLSGSIASIFRSFPMAIVGAMMIMVGIELAKFLESVRSSRDWV
                     VIGATVAFSIYFNMAAGFAAGIAVSWMMERWQKTDV"
     gene            4505..4714
                     /locus_tag="P5541_08475"
     CDS             4505..4714
                     /locus_tag="P5541_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRV72057.1"
                     /translation="MKLSARNALKGKIKEIKAGTVNTEVIVELKGGEEIVSMISKDSA
                     EKMGLKPGKEIYAVIKASSVMIGTD"
     gene            complement(4800..5978)
                     /locus_tag="P5541_08480"
     CDS             complement(4800..5978)
                     /locus_tag="P5541_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV72058.1"
                     /translation="MKRNNREKVPFNVWILGLVSLLNDTASEILYPLLPAFLTSVLGL
                     SPASIGLVDGISKSLEGITKGMGGALAQKTGRSRPFMVAGYIVSSAVKPLHAFAGSFW
                     GILGLRILDRGVGKGLRSAPRDALIAGSVPSGMLGRAFSLHRALDTTGAVIGPLFAFL
                     LLGHYGDVRQVFLWTIIPGALCVAATLLVKRESVLPPGERVPLSGWVGLLRGNRRFVF
                     FLLSTLLFTLANSSDLFLLMRTRELGLSETQVPLVWMLFNVAYVLAAYPVGLLADRIG
                     RGRLYLVGFVIYSLVYAGFAFHLSGMGVWMMMGFYGVYYGIAESLARSLVPDLVKSEM
                     RPLAYGVHHLAVSIGALPANLLFGFLWQTLGSSFAFVAGAFFSAIAGIVFFFGVERSQ
                     "
BASE COUNT         1599 a         1749 c         1737 g         1360 t
ORIGIN      
        1 gatactctcg gcccttgcgg gggatataat cctgatgcac atgaacaggc ccgagggcgg
       61 caccgcagaa ggggtaacat cggccgtacc ggtcctgaag gagaggggct tctccttcgt
      121 caggctctcg gaggtactta aataacaggg aaggaataat gattgccatg gatacagccc
      181 ttacaggaac atcccttctg atctacggta tggtgctggg gatctccata gccgcgcctg
      241 tcggccccat aggtgtactc tgcattcggc ggacccttgc cgcaggcctt ctccccggcc
      301 ttgcgaccgg ccttggagca gccgtcgctg acgggcttta cggagccgtg gcggctttcg
      361 gcatagcgtc cgtcgccaac cttcttatgg ctgtcaatct ctggctacgg cttgccgggg
      421 gaaccttcct tctctggctg ggcctcagaa ccgtttccag aaaaccggaa gatcctcttc
      481 aggagattcc gcaggacccc ggaggctttt ctcttgcgac agattttgct tccacttttg
      541 ttcttaccct ggccaacccc atgacgatta tttcgttttc ggccattttc atgggtctgg
      601 gaaaagacat cgcaacggca ggggcaacag gacctcttct cctagttatg ggcgtttttt
      661 ccggttccgc gctatggtgg cttggactaa gttcgttttt ctcccttttc aggaaaaaaa
      721 tgaccccgac agccatgaaa tgggtcaaca gggtttcagg gtctgttatt gtccttttcg
      781 gggctatagt tctccttgac gccatcattt gataaccccc cttcccgcaa gacagattga
      841 attgcccggg gggttccatt tttttgaacc tggatttatc atgacgtcaa aatagagagt
      901 aaaaggaagg tgataagcaa tgaaaggcaa attccttttg atggtccttg tcgcccttct
      961 cctcttcccc ctgccttgtg ccctcgcggc tgagtgcccc cccgagaaac cggacgcgga
     1021 accccttttc gagacagaag ggatattcgt cgggaaactg gatggcaacc ttgttatctt
     1081 cctggacaac cgcgacggcg acaggcaggt cagttttacc ctcgacaggg agcaggcctc
     1141 gcggcttttt tcatacgctg tcgaggcaag gaacgctgcg ccgggaatga accccttcgc
     1201 acccacggga aacatggaag taccgaaaaa acagagaccc atggaacctc ttatgctcga
     1261 aacccccgaa ggttcagccc tctgggccca cgtggcggga agggactacc ggaacagctc
     1321 cttcttcgcc gactttgtcc tggcaccgtc ggacagcgga ggttcacctg acaggaacta
     1381 catgcaggcc atgactatct acgagacagc accgactttc ccaaaagagg agattatcga
     1441 caaggcaacg ggacagccga ttggagaacc ggctcagccc gaggcaaaat ggggtgatga
     1501 gttttcagcg ttcctttcgg ctctgcaaaa gttcacgggc tcgaagttgc tgatcatcgg
     1561 atgacatcag gccgccgggg gttcactctg gtcgaactcc tggcggttct tatgatcgtc
     1621 ggcatcctgg cggggactat gttgctcgtc atgggatcgg gcacggagaa agcccgcctt
     1681 gccgtctgcc agagagacgg gcgcacagtg ggttctgcgg cgaggatcta cgtatgggaa
     1741 aatccgggga gtgaaccgga actgcaggac ctgatagact ccggccttat cgaaagggcc
     1801 cccaactgca tcggggaggg gacctgctcc tggtccccgg aagaactcag actcgtctgc
     1861 ggaagccagg aaccagccga accggagcct ctcaccccac tgggctcgac atttgccgag
     1921 atctcgggaa gcatggcggc aagggtcctg caatactacc aggacaacgg cacacccctc
     1981 agcacctggc aggatgagcg ctacgcagcg ataggacttg aaaagacctt ctgggacaac
     2041 cccgtcgaag ggataatgta tgttcctcag ggactcttcg tgaagatacg accggcagtt
     2101 ccggggataa cctttcaggt aacgacaacc ggtggagaga caaagacact tacaagcgac
     2161 ctcaactgga atatctggca caatgtagtc accgatacct ggtacttcca cagcagcaat
     2221 gattctgaca acgccttcga tccacagaca ctggtgataa tccgagactg atgctctacg
     2281 tcctctcacc tctcactgcg acaactatcc ccaaaaggat aagcgtgcca cctatgatct
     2341 tggcaagggt cagtgtctct ccaaacagga tccatgccag gagcgtggcc cctatcggct
     2401 cgcccagcag agctaccgcc acgaggctgg cgctcagata tctcagagcc cagttgtaac
     2461 tgctgtggcc gaggatctgg gataccagtg ccatcccgat gaaggccgtc caggtacttg
     2521 tgctgaaccc ttttaaaggt aatcctgcag caatgatgac caccagaagc acgaatgcgg
     2581 ccgcaccata acagacaaag gtgtaaacgg gcaggcttac cttggccctg acgacgcgcc
     2641 cggtaaggaa ataaagagtc gcgaaccagg caccgaaaag ggccaggatg tctccccaca
     2701 gggcccttcc acccagcgca aaatcaccgg cgctaatgac gagtacccca caaaagctca
     2761 gggcgatacc tgcccaggtc actcggctga ccctgtcgcc gctgacgaag ggggagagga
     2821 tcgccgtcca gacgggaatg gtctcgacga taaccacgct gctggctatg gatgtgtaga
     2881 acagcgaagt tatccatgta gaaaaatgca gggccaggaa ggtcccggca agtaccacac
     2941 gaagcaggtc cttcctttgc aatccgaaga gttcctgtct gtgccgtaca aacacgaggg
     3001 gggccattac agcggctgct atccccgagc ggtagaacga tattacgata ggcgaagcat
     3061 cggcgtatcg ggcaaagatg gaacctgtcg atatccctat gattccaagc aaaaggacaa
     3121 aaaagggcgt aaaacggcca acggatggcg acaagaaaaa aactcctctc gaggaaacgg
     3181 tttttcgggt aacatgatac tctgacagcc tctttgtaca aagagagaaa ttccttcagc
     3241 aaaggagacc gggaaacagt ttttcaaacc cgacccggag atcataatgt caagaattgg
     3301 tcctttcgat ttcagtcttc gcgaattcgg gggctccctg ggagacctgg ggaccctgtt
     3361 gcccctcgca gtaggataca tcaccgtctg cggtgtggac cctgcaggga tgctggtgtt
     3421 tctgggcctg gccaacatca ttactggagt catctacagg ctccccatgc ccattgaacc
     3481 catgaaggtc cttgctgtca tggccattgc ccaggcctgg agtcccggac agatctacgc
     3541 atctgccttt gcaatgggta ttacctggct tttccttggg ctatcggggg ccatcaactg
     3601 gatagctgcc cacactcctc attcagttat caggggtata cagttctccc tcgggatcct
     3661 ccttgcgatt cagggtttca ggatgatcca gggagcctgg tttctcgcgg cagtttctat
     3721 ccttattatc cttctcctga gggacaacag gttcgcgccg gcctcgcttg tactggtagc
     3781 aggaggcatt ctcctcatgg ccttcagagg cgacctctcc agcatccagc ccatgtccct
     3841 tgcccttccg cagctgctta aactcgacat gagccttcta tggccatcta tgagggatgg
     3901 cgggttctcc cagatacccc tgaccgcaac caatgccgta attgccacat cggccctcat
     3961 aacaaaatac tggcaggacc gcagcgtttc ggtcaggcaa ctctcactga acatgggcgt
     4021 catgaacctc ctgggcccct tcatcggggg cattcccctg tgccacgggg ctggaggcct
     4081 ggcagggcag tactacttcg gggcccgtac aggcggggcg aacataatcg agggctcgct
     4141 ggaaatattt ctcgggatat ttctttccgg ttccattgca tccatattca ggtcattccc
     4201 gatggcgatc gtaggggcaa tgatgataat ggtgggaata gaactggcca aattcctgga
     4261 atccgtaaga tcttcacggg actgggttgt aataggggca acagttgctt tctccatata
     4321 tttcaacatg gcggcaggat tcgctgcagg gattgcggtt tcgtggatga tggagcgatg
     4381 gcaaaaaaca gacgtataaa atatgcggca acagatcata tttgataagt aactggctgt
     4441 gtgataaaat tttgatgcga ataataagat aaacccctat gggtatttga gcggaggtgg
     4501 caatatgaaa ctcagcgcac gaaacgcact caagggcaag ataaaggaaa tcaaggccgg
     4561 aaccgtgaac accgaggtca tagtcgaact caagggcgga gaggaaatag tctccatgat
     4621 ctcaaaggac tcagccgaaa aaatgggtct caagccgggg aaggaaatct acgccgtcat
     4681 caaggcttcc agcgtaatga taggaacgga ttgacatttg aacaggatat gaaagtgtaa
     4741 aatgaagcca tgggatatcc cggggcttca ttttttgtct tgggtaatat cttcctggac
     4801 tattgagacc tttcgacccc gaagaagaac actatgccgg cgatggccga aaagaacgct
     4861 cctgccacaa aagcgaaaga agaccccagc gtctgccaga ggaacccgaa aagaaggttg
     4921 gcaggaagcg cccctatgga aacggccagg tgatggactc cgtaagcgag ggggcgcatc
     4981 tccgatttga cgagatccgg gaccagggac cttgcaaggc tttcggcaat gccgtaatag
     5041 accccgtaaa accccatcat catccatacg cccatcccgg aaaggtgaaa cgcaaaaccg
     5101 gcgtaaacaa gggagtagat gacgaaaccc accagataaa gccgacccct gccgatacga
     5161 tctgcaagca ggcccaccgg ataggcggcc agcacatagg ccacgttgaa aagcatccat
     5221 acaaggggca cctgggtctc tgaaaggcca agctcacggg ttctcatcag caagaacagg
     5281 tcgcttgaat tggcaagggt gaaaagcagt gtggacaaca ggaagaacac gaatctcctg
     5341 ttccccctca aaagaccgac ccatccggaa agggggaccc tttctcccgg cggcaggacg
     5401 ctctcccgct tcacaagtaa ggttgcagca acgcataaag cccccggaat gatggtccaa
     5461 aggaaaacct ggcggacatc tccgtaatgg cccagaagca ggaaggcgaa caatggtcca
     5521 atgacggcac cggtagtgtc cagtgccctg tgaagggaga aggcccgtcc cagcatcccc
     5581 gatggcacgg aacctgcgat cagggcatcc ctcggagcgc tgcgaagtcc tttcccgact
     5641 ccccgatcca gtatcctcag gccaagtatt ccccagaagg acccggcaaa ggcatggagg
     5701 ggcttgaccg ctgaggagac aatgtaacct gctaccataa agggcctaga acgtccggtc
     5761 ttctgggcaa gggcgccccc catccctttg gttatcccct caaggctttt agatatcccg
     5821 tcgaccaacc ctatcgatgc cggtgaaagc ccgagaaccg acgtaaggaa cgccggaagg
     5881 agcggataca atatctcgct cgcggtatcg ttgagaaggc ttaccaggcc aagtatccag
     5941 acgttaaaag gtaccttctc cctgttgttc cttttcatca cccttcctcc ggtatgccag
     6001 gatcataagt cggaaccgaa aggattttac acccttcctg tcggaaatga ctcctttttc
     6061 aaggaatatg acactggtca aagtccgcgg cttttatcag tccattttta ggccccgtta
     6121 atcgggaatc gtgaatcgaa taaacccggg agactggaag gggcaaggtc aggggcgtaa
     6181 acggtgaacg gtcaacgtga catcaggaga ttggaagccc aagggcttgt cattctgagc
     6241 aaagcgaaga atctggactt actataatcg attcaacatc cacctgcggg aaaatgggtg
     6301 taaatttact gcgggaatct gggtgttaat tgacaaaaga ctcaaaagtg taggtgcgac
     6361 cccctatgag gttaaaagtg caggcctgat cccgctgggt ttgcatataa ggtttttgca
     6421 ttattcgtat tgtatttatc aataa
//
LOCUS       DAOTIQ010000083         6396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_14396_length_6396_cov_7.498817, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000083 DAOTIQ010000000
VERSION     DAOTIQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14396_length_6396_cov_7.498817"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..787)
                     /locus_tag="P5541_08485"
     CDS             complement(116..787)
                     /locus_tag="P5541_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRV72059.1"
                     /translation="MKVKCVAFDFGGVIAEEGFYNGMKAIALRFGLDPEETVDMAFRL
                     VFEAGFVEGGCTEDELWDLFRKETGISARDRDLREMVLSRFIVRPWMLQLARSLREDG
                     IITAMLTDQTEWLKELDGRAPFLDLFDPVLNSWDTGKTKKDPSSFTDLASAVGLKPEE
                     ILFIDDNEGNVERASTMGLETILYRDRESFEKEMDTKIFKALTVNSESEASGDGHQDA
                     GEARA"
     gene            856..2193
                     /locus_tag="P5541_08490"
     CDS             856..2193
                     /locus_tag="P5541_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV72060.1"
                     /translation="MMEGASCMVSGSSRTKFFTDSARRYRWVVWSVMALAYMVVFFHR
                     LAPGVVRDDLTLAFGISGAAFGNFASMYFYSYLLMQIPVGMLSDSLGARITATAGIFL
                     SGIGSILFGLAPSIGWAYAGRFIVGIGVSTVFVCILKVLSEWYSGKEFATMSGATAFV
                     GNFGGLVAQTPLALLVGIFTWRFTFAAIGALSFLLAVLCFVLIRNRPEDAGFDPVNLS
                     GPGDSPPMTELFPAVRVAICSRAMWPTMTLASLFAGAQLAFTGAWGVPWLTDVYEMTR
                     QEASGLVSIVVVGAMIGGVLVGKLSDLVGARRWPLIFVSFFNVAAWGVIVFWGSGKPP
                     LEVLKPALFIMGVASMALVVCLAVVKETNDPRYTGVALSVLNMGVFVGIAAYPPAMGA
                     VIDFFSSCSPIVQYRWALFLCFAGAAAGFILSFAVPETGCQNVAADRSRPPRG"
     gene            complement(2204..3001)
                     /locus_tag="P5541_08495"
     CDS             complement(2204..3001)
                     /locus_tag="P5541_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HRV72061.1"
                     /translation="MRLRNVTAAALAVIVLFAGTCLAESANHFNGCIEGDSSGTILFR
                     AFAEGLKPESMEMILDEEPDDSGRVRHMYLDLYGCDLGGVRIDRLEIEAVDVQFTPTC
                     EWKESGPDIENMLAVYAQAVILEKDVNEAISDEAFGDGDEHWHDVTLDFRENGVYVKG
                     YYLANFIFRLDILIELEGRFGIVGGKQVWLEDYTMRVNRVDLPDFITERAIGKIQPII
                     DLEDFVFPLTLREVVQEDDRVTLRSRVLPRRIEGILYKYQADPADKE"
     gene            3138..4070
                     /locus_tag="P5541_08500"
     CDS             3138..4070
                     /locus_tag="P5541_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV72062.1"
                     /translation="MEDRKKVMFADGAMFTIAIFWGSGFGVTNWLLGYISPLWLLTAR
                     FVLSSLMLLAVFRGRLKYLGRRDIFLGVMLGLLLSVTFVFHIMGLVFTSPGKQSFIAA
                     SNVVMVPFLFALFYRKLPSIIATVGAFLTTAGLLVMAFTPGMRFNLGDFFSLVLAFGI
                     ALHVLSVGNLSRRMDPIALTVVQLVSATLVLLFSALIFEPFPDFTAVDPRALWGVLYV
                     AVFVTVIPFLVQTMAQRYSPEIHAAILLSLEGPFGYLIAVFIGQEILNSQVLLGGGII
                     VAGVILAECDTFIRKSFHLPGMTDSGAPDQGEEN"
     gene            4073..5245
                     /locus_tag="P5541_08505"
     CDS             4073..5245
                     /locus_tag="P5541_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV72063.1"
                     /translation="MQRVRRLLPRMAGSSFAIMSFSYVYFLLPLYLDNAGLTSPSTIG
                     WILGTYYAASTLPRPLMALLIERFPFRITLTGAALCCLAGSAGLAFSGTSMLFLVLSR
                     IMMGFGFGLFLVSLTSYQTMSIPDDVRGAAFSLITIGAMAPFLLVIPLVEWLLQNGFF
                     GFYIWVGPIVAATCAAVGMTFGNIDRNRTGSGSRWGTYTQLFAIPGARALFVSVFVYG
                     MTDAAIISISTLSMSSGLLSSWFMSSFAVGAILIRLLGFRYMDIFPKDRMFAIVIAVT
                     SFSLFGTAFSSSNVAFSLWGFIYGMGVGVGFPLSLAMIGDVAPPELCPKATAMVWLIL
                     DGCFFITPVVMGYASSVLGVAGAFRVIPGVLFFAAPAVYFSLWKPLSSSKKGPLPG"
     gene            complement(5234..6325)
                     /locus_tag="P5541_08510"
     CDS             complement(5234..6325)
                     /locus_tag="P5541_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRV72064.1"
                     /translation="MKKTNQVILLLIGFFCLSLQTASANGRSGIGLDPEVDQTGLKVI
                     SVEEGSPFFTAGLRPGDKILEVDFKSISEMPIARAVEEVEAASLPGRSAILTLEGTDG
                     ERIVRVCAPPSDPRTRSFLAAENDILDAWAMSSEGWDLFIEAACGFALGQIGRQQFFR
                     ELRSSRKFFSDSRNRLLAISIPTTLPPAVREELTLSKSRLADANTLRFFAARILYRSM
                     LSGEAIRTEGETRPQEYLAAWLVQNIKSRLVSETGVPGLERAWSYLRTLYLQASAPPE
                     EGEPLPEKLAKEADMALSEARDRIERISDLGILAEETLSYTAATEQAAAVLLGKMLLR
                     YSEIERKANIASGQGFGHLLRARTIILSR"
BASE COUNT         1241 a         1696 c         1680 g         1779 t
ORIGIN      
        1 agacaggaca cgggaggcag gagaacctga aaaaaccaat tcagattctt cgcttcgctc
       61 agaatgacaa agccttcgtc cttgggtttt cgagtcacga gtcacaagtc acaagtcacg
      121 cccttgcctc tccagcatcc tgatgcccgt ctcctgacgc ctcgctttca ctattcaccg
      181 ttaacgcctt gaagattttc gtatccatct ccttctcgaa actttccctg tccctgtaaa
      241 ggatggtttc cagccccatc gtggaggccc tttcaacgtt gccctcgttg tcgtcgatga
      301 acaggatctc ttcaggtttc agacccactg ctgaagcaag gtcggtgaag gaagaagggt
      361 cctttttggt ttttcccgta tcccagctgt tgaggacagg gtcgaagagg tcaagaaagg
      421 gagctctccc gtccagttct ttcagccatt cagtctggtc cgtaagcatc gcggttatta
      481 ttccgtcttc ccgcagggat cttgcaagtt gcagcatcca tggtcttacg atgaacctcg
      541 acagaaccat ttcccgaaga tcccggtccc gggcgcttat gcctgtctct tttcggaaaa
      601 gatcccagag ttcatcctcg gtgcagcctc cctcgacaaa acctgcttcg aaaacaagcc
      661 tgaaagccat atcaacagtc tcctccgggt caagcccgaa acgcagggcg atagccttca
      721 tgccattgta gaaaccctct tcggctatga ctcccccgaa atcgaaagcg acgcatttga
      781 ctttcatgtt attcctccac ccctaaggtt gtatagtaat gtactggatt cagacccgag
      841 aagtataaca tcgcgatcat ggaaggggca tcctgcatgg tttccggatc ttccagaacg
      901 aaatttttca cggacagtgc ccgccgttac aggtgggttg tatggagcgt catggctctg
      961 gcctacatgg tggtcttttt ccaccgattg gccccagggg ttgtcaggga tgatctcaca
     1021 ttggctttcg gcatatcagg cgctgccttc ggaaattttg cctcaatgta tttttattcc
     1081 tatctgctga tgcagatacc ggtgggaatg ctttcggatt ccctcggtgc caggatcacc
     1141 gcgacggcgg gaatattcct ttcaggcata ggatcgatat tgttcggact cgcaccatcc
     1201 atcggctggg cctatgcggg aaggttcatt gtcggaatag gcgtttctac ggtattcgtc
     1261 tgcattctca aggtgttgtc cgagtggtac agcggcaagg aatttgcgac catgtcggga
     1321 gctacggctt tcgtggggaa tttcgggggg cttgtggcgc agacacctct cgctcttctg
     1381 gtagggatat tcacctggcg ttttaccttt gccgccatag gggcactttc tttcctgctt
     1441 gcggttctgt gttttgtcct tatcaggaat cgcccggagg atgccggttt tgatcctgta
     1501 aacctctctg gtcccgggga ctccccgccg atgacggagc tttttcccgc tgtcagggtg
     1561 gcgatatgtt cacgggcaat gtggccaaca atgaccctgg catctctttt cgcgggggct
     1621 caactggctt ttaccggagc gtggggtgtg ccctggctca ccgatgtcta tgaaatgacg
     1681 cggcaggaag cctcagggct ggtttcgata gtggtcgtag gtgccatgat aggtggcgtt
     1741 ctggtcggca aactttcgga tcttgtgggt gcaaggagat ggccactgat ctttgtgagt
     1801 ttcttcaatg ttgccgcctg gggggtgata gtcttctggg gttcgggtaa gcctcccctg
     1861 gaggtcctca agccggcgct ttttataatg ggtgtagcct cgatggctct cgttgtatgc
     1921 ctggccgttg tgaaggagac caatgatccc agatatacag gggtggctct ttcggtcctg
     1981 aatatggggg ttttcgtagg tatcgctgcc tacccgcccg ccatgggagc cgtcatagac
     2041 ttcttctcgt cctgttcccc gatagtgcag tacaggtggg ctttgtttct ctgcttcgcc
     2101 ggggccgcgg cagggtttat tctttctttt gcggttcccg agacgggatg ccagaacgtg
     2161 gcggccgatc gttcccgacc gccacgggga tgaaccgccg gttctattcc ttgtcggcgg
     2221 gatctgcctg gtatttgtaa aggattcctt cgatcctccg gggaagcact ctgcttcgga
     2281 gggtaactct gtcgtcttcc tgaacaactt ccctcagtgt cagagggaag acgaaatctt
     2341 cgagatctat tatgggctgg atcttcccta tggctctctc tgtaatgaaa tcgggcagat
     2401 ccaccctgtt gaccctcatg gtgtaatcct cgagccagac ctgcttcccg ccgactatgc
     2461 cgaaacgtcc ttcaagttcg atcaggatgt ccagacgaaa gatgaaattg gcaagatagt
     2521 aacccttgac gtatactccg ttttcccgga agtccagtgt aacatcgtgc cagtgctcgt
     2581 ctccgtcacc gaaggcctcg tctgaaattg cctcgttgac atctttttcc agtatgacgg
     2641 cctgggcgta gacggcaagc atgttctcaa tatcggggcc cgattctttc cattcgcatg
     2701 ttggagtgaa ctggacatcg accgcttcta tctccaggcg gtctatccgg acccctccga
     2761 ggtcgcatcc atacagatcc agatacatgt gccggacccg tcccgagtca tcgggttcct
     2821 cgtcgaggat catttccatg ctttcaggct tgaggccttc ggcaaaggcc cggaaaagaa
     2881 tagttccgga agaatcccct tcgatgcagc cgttgaagtg attggcgctt tcagccaggc
     2941 aggttcccgc aaacagtaca ataacggcaa gagccgctgc tgttacgttt ctcaggcgca
     3001 ttgttccacc tccgtaagaa tagcagacat ggattccgtc agaagatgat accatacacg
     3061 aatttttgta cacaaccggg gtttgtcgtg atatgctttc ttttcgggga acaggcaatg
     3121 aaaggctggt gtgttttatg gaagacagaa agaaggtaat gtttgcggat ggggcaatgt
     3181 tcactattgc tattttctgg gggtccggtt tcggtgtcac caactggctc ctgggttaca
     3241 tctctcccct gtggcttctg acggcgcgtt ttgtgctgag ttcacttatg ttgctggctg
     3301 ttttcagggg tcgtctcaaa tatcttggaa ggagagatat ctttctcggt gtgatgcttg
     3361 ggctacttct ctcggtaacc tttgtgtttc atattatggg tctggttttt acgtcccctg
     3421 gcaaacagtc tttcattgcc gcgtccaatg tcgtgatggt tccttttctc ttcgctcttt
     3481 tctacaggaa gttgccttcc attattgcca cagtgggagc ctttctgacc acagcggggc
     3541 ttcttgtaat ggcttttact ccgggtatga gattcaacct tggtgatttt ttctctttgg
     3601 tgctggcttt cgggatagca cttcacgtcc tttccgtagg aaacctgagc cgtagaatgg
     3661 atccgatagc gctgactgtg gttcaactgg tctcggcgac gctggtcctg ctcttttccg
     3721 ccctgatctt tgaacccttc cccgacttta cagcagtgga ccccagggcc ctctgggggg
     3781 ttctatacgt ggccgttttt gttacagtga tcccctttct tgtccagacc atggcccaga
     3841 gatacagtcc cgagatccac gctgcgatcc tgctctctct cgaaggcccc tttgggtacc
     3901 ttattgcggt attcataggg caggaaatcc ttaacagcca ggtgctcctg ggaggtggca
     3961 tcatcgttgc gggagtgatc ctggcagagt gcgacacttt tatcaggaaa agctttcacc
     4021 tgccgggaat gaccgatagt ggagcccccg atcaagggga ggaaaactga aaatgcaaag
     4081 ggtacgcagg ctacttccga gaatggcagg cagcagtttt gctatcatga gcttcagtta
     4141 cgtctatttt ctgcttcctc tctacctgga caacgcaggg ctaacttcac cttccaccat
     4201 aggatggatc ctgggaacct actatgccgc atcgaccttg cccaggcctt tgatggctct
     4261 tctcatagag agatttcctt tcaggattac ccttacaggg gccgcattgt gctgtctcgc
     4321 gggaagcgcg ggtcttgcct tttccgggac atcgatgctg ttccttgtcc tttcaaggat
     4381 catgatgggt tttggttttg gcctttttct ggtttccctg acatcctacc agaccatgtc
     4441 catacctgac gatgtgaggg gagctgcctt ctctcttata acgataggcg ccatggctcc
     4501 cttccttctt gtgatccccc tggttgaatg gctcctccaa aatggttttt tcgggtttta
     4561 tatctgggtg gggcccattg ttgccgcaac ttgcgccgcc gtcgggatga ccttcggaaa
     4621 tatcgacaga aacagaacgg gatccggcag ccggtggggg acctacaccc agctgtttgc
     4681 cataccggga gcccgggcgc tttttgtctc tgtttttgtt tacggcatga ccgatgccgc
     4741 aataatatcc atttcgactc tttcaatgag cagcgggctg ctatcttcct ggttcatgtc
     4801 ttcctttgca gtcggagcta tcctgatccg gctcctgggt ttccgttaca tggatatatt
     4861 tcccaaagac aggatgtttg caattgttat agcagttact tccttctcgc ttttcgggac
     4921 cgctttctct tcgtcaaacg ttgccttctc tttatggggg tttatctacg ggatgggggt
     4981 gggggtaggg tttcctctgt cgctcgcgat gataggggat gtggctcccc ccgaactttg
     5041 cccaaaggcc accgcgatgg tttggttgat cctggacgga tgttttttca taactcctgt
     5101 cgtaatgggg tacgcctctt cggttctggg tgtggccgga gctttcaggg tcattccggg
     5161 agttctcttt tttgccgctc cagcggtata tttttccctc tggaaacctc tttccagttc
     5221 taaaaaaggt cctttaccgg gataatatta ttgtccttgc cctgaggaga tgtccgaaac
     5281 cctggcctga agcaatgttt gccttccttt cgatctcgga gtagcggaga agcatttttc
     5341 ccagaagaac ggcggcggcc tgttccgtgg cggccgtata ggaaagtgtt tcttccgcaa
     5401 ggatcccgag gtccgagatc ctctcgatcc ggtctcttgc ctcggataaa gccatgtctg
     5461 cctctttcgc cagtttttcc ggcaggggct ctccctcttc agggggcgcg cttgcctgaa
     5521 ggtaaagggt tcgaagataa ctccaggccc tttccagacc gggcacccct gtttccgaca
     5581 caagccggga ctttatgttc tggaccagcc atgcggcaag gtattcctgg ggccgcgttt
     5641 ctccttctgt ccttatggcc tctccggaaa gcattgatct gtagaggatc cttgccgcga
     5701 aaaacctcag ggtattggca tcagcaagcc tggatttaga caaggtaagt tcttctctca
     5761 cggcaggggg cagtgtcgtc gggatactga tcgccaggag cctgttcctg ctatcggaga
     5821 aaaactttct tgaagaacgt aattccctga aaaactgctg ccggccaatc tgcccgaggg
     5881 cgaacccgca tgccgcctct atgaagagat cccatccttc ggaagacatt gcccaggcat
     5941 cgagaatgtc attttctgca gccagaaaag atctggtccg ggggtcggac gggggggcgc
     6001 acaccctgac gatcctttct ccatcagttc cttcaagagt aagaatggca gatcttccag
     6061 gcaacgaggc tgcttccact tcctctactg cgcgcgcgat cggcatctct gagatagatt
     6121 tgaagtcaac ctcaaggatc ttgtcccccg gacggagacc tgctgtgaaa aaagggctcc
     6181 cctcttccac ggagatcacc ttcaggcccg tctggtcaac ctccgggtca agtcctattc
     6241 cggaccgccc atttgctgat gcggtctgga gagacagaca gaaaaatcct atcaggagga
     6301 gaattacctg gtttgttttt ttcaattcgg cccccgatcg atgttatgtg acaccttcgt
     6361 ccatgatatt attaaacaaa tgctccgcaa aggaaa
//
LOCUS       DAOTIQ010000084         6256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_14825_length_6256_cov_7.258668, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000084 DAOTIQ010000000
VERSION     DAOTIQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14825_length_6256_cov_7.258668"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1486)
                     /locus_tag="P5541_08515"
     CDS             complement(290..1486)
                     /locus_tag="P5541_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV72065.1"
                     /translation="MSVPFAERTKRLGTETAFAVGAEAASLAATGVKVYPFHLGDLNI
                     PTPEHIVEAANRAIREGKTSYCPTPGITPLREAMAEEIYRTRGFKIDADNVSIQTGGK
                     PVIGKFIMTLMNPGDEVLYPNPGFPIYESMINFHEGVGKPYGFRETDKGFSLDLEAIE
                     RQISPRTKLIIYNNYHNPTSAESDMEEMEHLADICLKHNIYVLSDEAYFDILYDGTPK
                     SITRFQGLPERTVILYTFSKKFAMTGWRLGAAIGPREVIEAISRFNVNDESCTNNFVQ
                     WAGVAALRGPLDDHRKMIDILRERRDTALSILNNTKGIHVATPDSTFYLFPNVTKAME
                     KLGMEEVEDFRKFVLEKTGVSFTTRNHFGTPLPGETQKYIRLAYSGIDTPEIIEGLGK
                     MKELLG"
     gene            complement(1494..2474)
                     /locus_tag="P5541_08520"
     CDS             complement(1494..2474)
                     /locus_tag="P5541_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV72066.1"
                     /translation="MKEQFLSPKIFSQPEMVPTRFGYGDGLVELGKNNPSVVVLGADL
                     SSSLCVDRFRNAYPERFIQTGIAEQDMMALAAGLALSGKVPFVSTYGVFCTGRAWDQL
                     RTTVAYARLNVKIGSGHGGVSVGADGATHQALEDISITRTIPNVTVIVPADYHETFKA
                     TVAAASISGPVVIRFGREKVPVITTDRTPFTVGKAQIFKNGTDVTIAACGQMVYESLL
                     AAEALAKDGISARVINCHTIKPIDSKTLIRAAEETGAVVTAEEHQIMGGFGSAVAEVL
                     SQNCPVPIRMIGVQDTFGQSGDPDDLMEEYCLRAGSIVKAAVEAVGMKKG"
     gene            complement(2471..3304)
                     /locus_tag="P5541_08525"
     CDS             complement(2471..3304)
                     /locus_tag="P5541_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV72067.1"
                     /translation="MEEAKLRHLKSVSLAIRQDIVRMVSEAGSGHPGGSLSIADIVTV
                     LFFDVMKHDPADHCWDGRDRFILSKGHACPAVYAALAESGYFPKEELVTLRKMGSRLQ
                     GHPDMCKLPGLEASTGSLGQGLSIASGMALAMKMDKKPNRVFCIMGDGELDEGQIWEA
                     AMTAAHYRLDNLCGIVDRNGLQIDGNTDDVKTLEPLSDKWRAFGWNVIEVDGHNLREL
                     SDAMHRFRHTHFRPTVIIAHTIKGKGVSFMENQAGWHGKAPSPEQAKAALHELSREVI
                     R"
     assembly_gap    3581..3680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4254..4787
                     /locus_tag="P5541_08530"
     CDS             4254..4787
                     /locus_tag="P5541_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV72068.1"
                     /translation="MFVLDLLRKRRSIRKFQDRPLTPEQIRVLEEAVLRSPSARNLRS
                     WEFIFVDDRDLLVKLADTRGSSSAFIAGAALGVVIIGDEKTVDTWVEDGSIAAIIIQL
                     AAADLGLGSCWAHVRNREHAPGKSAEDHVRELLGIPDPLKVLCVIAVGYPAEQKPGIP
                     KEELSSGKIFRNRYGQV"
     gene            complement(4838..>6256)
                     /locus_tag="P5541_08535"
     CDS             complement(4838..>6256)
                     /locus_tag="P5541_08535"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRV72069.1"
                     /translation="NSGSSEGCEEMTERDLLERYKSRIISNLQDMVRIRSVEGTPEEG
                     KPFGDGVHQVLNLALETASELGFRTVNHDGMIGYAEYGSGKEMVAVLGHLDIVPEGKG
                     WTYPPFGAEIHDGKMFGRGTIDDKGPTIAALWALKILSDEDHRLSRRIRIIFGTNEES
                     GFRGIKWYAANEEAPVAGFTPDIHEGIVHAEKGGLHIGIDVPVGEHEGISLISFRAGE
                     AVNIVPDQCEAEFEGDPAALEKWAGVLRGALDSTPLGGEVVLEKRTLSAKVFGKAAHA
                     SLPEKGENAIAALCRLIDANPPSHRPWEAFKVISGLLCDPRFGSDLGISMSDDVSGPL
                     TCNPGLASFSKGVLSVVLDIRHPVTVKGDLVVERMKEKLAPLGLTPVERSRNTPLFVP
                     KDSPLIKDLSEAFFETTGKHPKVMAIGGGTYAKALPNVVAFAPFPSGLPDLAHQADEY
                     IGIEDLLSSVLVVKAAMLRLAR"
BASE COUNT         1336 a         1647 c         1715 g         1458 t
ORIGIN      
        1 cgcaatgaca agatcctgct cctgttttcc cagtcataag tcacgagtta agagttgcgc
       61 ccttgatcct gcccttcctg ttgccttcag cctgggttgc gccccgtagg ccgaaggccg
      121 gaggcccctc gttaaaactc ctccctcgtt aattcgtctc gggaccttgt cgggaccaag
      181 tccttcagct tgcctcgagg gtatgccgcc ttgctcttcg gccttctgtt cactgtttac
      241 ctccacagag accgcgggac ttcgcgttga ccgtttacgc gtttcgctcc taccccagca
      301 gttccttcat cttcccgagt ccttcgatga tctcgggggt gtcgatcccc gagtaggcca
      361 gtcgaatgta cttctgggtc tcgcccggca agggtgtgcc gaagtggttc ctggtggtga
      421 acgagacgcc agttttttcc aggacgaact tcctgaagtc ctcgacctct tccattccca
      481 acttctccat agccttcgta acgttcggga agaggtagaa agtgctgtcg ggggtagcga
      541 cgtggatacc tttcgtgttg ttaaggatgg ataaggctgt gtcgcgtctt tccctgagga
      601 tatcgatcat tttacggtga tcgtccagtg gtcctctcaa ggcggcaacg cctgcccact
      661 ggacgaagtt atttgtgcag gattcgtcgt tcacgttgaa acgggagatg gcctcgatca
      721 cctccctggg accgatcgcc gctccaaggc gccaacccgt catggcgaac ttcttggaga
      781 aggtgtagag gatgaccgtc ctctcgggaa gcccctggaa tcgggtaata ctcttggggg
      841 taccgtcata gagaatgtcg aagtatgcct cgtcgctaag cacgtaaatg ttgtgtttaa
      901 ggcagatatc ggccaggtgt tccatctctt ccatgtcaga ctcggcgctg gtcgggttat
      961 ggtagttgtt gtagatgatg agctttgtcc gaggggagat ctgacgttct atcgcttcca
     1021 gatccagtga gaaacccttg tcagtctccc tgaagccgta aggttttcct acaccttcgt
     1081 ggaagttgat catcgattca tagatgggga accccgggtt ggggtagagt acttcatcgc
     1141 cggggttcat gagggtcatg atgaatttgc cgatcaccgg tttcccgccg gtctggatgc
     1201 tcacgttgtc ggcgtcgatt ttgaaccccc tggtcctgta gatctcctcg gccatagcct
     1261 ctctcagggg cgtaatacca ggggtgggac agtagctcgt cttgccttcc ctgattgccc
     1321 ggttggctgc ctccacgatg tgttctgggg tagggatgtt aagatctccc aggtgaaagg
     1381 ggtaaacctt cacccctgtg gcagccagac tggccgcttc ggcaccaacg gcaaaagccg
     1441 tctctgttcc tagtcgtttt gttctttcag cgaagggaac tgacatggag agatcaccct
     1501 ttcttcattc ctacagcttc aacagcggct ttcacaatgg atcctgccct gaggcagtac
     1561 tcttccatca ggtcgtcggg atcaccggat tgtccgaagg tgtcctggac cccgatcatc
     1621 cttatcggca cagggcaatt ctggctgaga acttccgcaa cggcgcttcc gaagccgccc
     1681 attatctggt gttcctctgc tgtcacaaca gctcccgttt cttcggctgc ccttataagg
     1741 gtttttgaat cgatcggttt aatggtatgg cagttgatca cccttgcaga tatcccatct
     1801 ttcgcaagtg cttcagccgc caaaagcgat tcgtagacca tctgtccaca ggccgcgatc
     1861 gtgacgtcag tgccgttttt gaaaatttgg gctttaccca cggtaaaggg cgtcctgtct
     1921 gtggttatca caggcacctt ctccctgccg aaccggataa ccaccgggcc ggagatagat
     1981 gccgccgcaa cggttgcctt gaaggtctca tgatagtccg ctggcactat caccgtaacg
     2041 ttcggtatgg tcctggtaat ggagatgtct tcaagggcct gatgggttgc gccgtcggct
     2101 ccaacggaga ccccgccgtg tccggacccg atctttacgt tcagcctggc atatgccact
     2161 gtagtcctca gctggtccca ggctcttcct gtgcagaata ccccgtaggt gcttacgaag
     2221 ggcaccttgc cggagagagc cagccccgcc gccagggcca tcatgtcctg ctcggcgatc
     2281 ccggtctgga tgaaccgttc gggataagcg ttcctgaagc ggtctacgca gagagaagag
     2341 ctgagatcag ctccaaggac gaccactgag gggttgttct tccccagttc aaccagtccg
     2401 tcaccgtatc cgaaacgggt ggggaccatt tcaggttggg aaaagatctt cggagaaaga
     2461 aattgttctt tcatcggatg acctcccgtg aaagctcatg aagtgccgcc ttggcctgct
     2521 ccggtgaagg ggcctttccg tgccatccgg cctggttctc catgaaggaa acgcctttgc
     2581 ccttgatggt atgggctatt atgacggtgg gcctgaaatg tgtgtgacgg aagcggtgca
     2641 ttgcgtcgga aagttcgcga agattgtgac cgtccacctc gataacgttc catccgaagg
     2701 cccgccactt gtcggaaagg ggttcgaggg tctttacgtc gtcggtgttg ccgtcgatct
     2761 gcagtccgtt cctgtcgacg atgccgcaga gattgtccag cctgtaatgt gcggcggtca
     2821 tagccgcttc ccagatctga ccttcatcga gctccccgtc gcccatgata cagaataccc
     2881 tgtttggctt tttgtccatt ttcatggcga gagccatccc ggaagcgatc gaaaggcctt
     2941 ggccaaggct tcctgtcgag gcttccagcc ctggcagttt gcacatatcg gggtgtccct
     3001 ggagcctgga acccattttt ctgagggtca cgagctcttc cttagggaaa taaccgcttt
     3061 cagccaatgc ggcgtagacc gcagggcagg cgtgcccttt tgaaagtatg aaacggtctc
     3121 gtccgtccca acagtggtcg gcagggtcgt gcttcataac atcgaagaaa aggacagtga
     3181 caatatcggc gatcgacaag gatccgcccg gatgtcctga acccgcttcc gaaaccatcc
     3241 tgacaatgtc ctgtctgatg gcaagggaca ccgacttgag atgacgcagt ttggcctctt
     3301 ccatgtcttg acctcccggg aaatacggtg aatggtatta ctcacatata aacaatatca
     3361 gaggaagtgg aaaaaacaag gatgggaatt cgacgataat gaatcttgaa ttgaatcagg
     3421 aaaacctggt caaaggcaaa accttttcac cgcagagaac acagagggcc gcagagaagt
     3481 acgaagatat ggaaagaacg taaagacaat gaaagagacg aaggggtgga aaaaacaagg
     3541 atgggaattc gacgataatg aatcttgaat tgaatcagga nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn aaaacctttt caccgcagag aacacagagg gccgcagaga
     3721 agtacgaaga tatgggaaga acgtaaagac aatgaaagag acgaaggggt ggaaaaaaca
     3781 aggatgggat atcaaaaggg cgtaaacggt aaatggttaa cggtgaactg aaagaacaaa
     3841 aacctgtgag acatgagaag taagaagaga agggatttac gattcacgct tcaatattca
     3901 agatagactt tttcccccgt ttacgtcttg atattgatct tgccccttgc atttcaataa
     3961 caaatggtgt atcatcctga tagcggggag aagtgttccc cggagtcggg ctttccggcc
     4021 tgtaattgcg ggtgtaggga aagtaaaagc agcggatagt ttgaccgggt tcagttggaa
     4081 gtcaggtctg gttgcaaatt ggcagcaaaa ggctgttgac ggattaggga gtggttgcct
     4141 gggaagtgca tctctatgcc ggagagcccg accatgaaag agacgggggc ggtatatacc
     4201 gccccctttt ttcgtcgttc cgacggctat ttacccctta agagagagag atgatgttcg
     4261 tgctggacct tttgcggaaa agaagaagta taaggaaatt ccaggaccga cctcttaccc
     4321 ccgaacagat aagggtcctg gaggaggctg ttctgaggag cccctctgcc aggaacctga
     4381 ggtcatggga gttcattttt gttgatgaca gggatctgct ggtcaaattg gccgatacaa
     4441 gggggagcag ctcagctttc atagcggggg ctgcccttgg ggtagtaatc attggggacg
     4501 aaaagaccgt cgatacctgg gttgaggacg ggtccatcgc ggccattatc atccagcttg
     4561 cagccgctga cctcggactg gggtcatgct gggctcatgt gcgtaacaga gagcatgccc
     4621 cgggaaaatc cgctgaggat catgtgagag agctgcttgg gatcccggat cctctgaaag
     4681 tgctctgcgt gatcgccgtg ggttacccgg ccgagcaaaa gcccgggatc ccgaaagagg
     4741 aactgtcctc cggaaagatc ttcaggaaca ggtacggcca ggtctgaaca aagggagggg
     4801 cgtctgcacc ctccctgtct ctgatcctgt tcgtacccta ccttgccagc cttagcatgg
     4861 cggccttgac gacaaggacc gaagagagaa gatcttcgat cccaatgtat tcgtctgcct
     4921 ggtgggcgag gtcgggaaga cccgacggga acggggcgaa ggccaccacg ttggggaggg
     4981 ccttcgcata ggtccctcct cctatggcca tcactttcgg gtgcttcccc gttgtctcga
     5041 agaaagcttc gctgagatcc ttgataagag gcgaatcctt gggcacaaac aggggagtgt
     5101 tcctcgatct ctctaccggg gtcagcccga ggggagcaag cttttccttc atcctctcca
     5161 ctacaaggtc gccctttacg gttaccgggt gccttatgtc aaggactaca gataagacac
     5221 ccttcgagaa agaggcaaga ccggggttgc aggtcagggg acccgagacg tcatcggaca
     5281 ttgagatccc cagatctgat ccgaaccttg ggtcacagag aagcccggag atgaccttga
     5341 aagcctccca ggggcgatgg gaggggggat tggcatctat cagcctgcag agcgctgcaa
     5401 tggcgttctc tcccttttcg ggaagactgg catgggccgc cttgccgaag actttcgctg
     5461 aaagggtcct cttttcgagt accacctccc ccccgagggg tgtggaatcc agggctccac
     5521 gcagaacgcc cgcccacttt tccagggccg caggatcacc ttcgaactcg gcctcgcact
     5581 ggtccgggac gatgttcacc gcttctccgg cacggaagga gatcagactg atcccttcat
     5641 gttccccgac gggcacatct atcccgatgt gaagaccacc cttctcggcg tggactatgc
     5701 cttcgtggat gtcgggagtg aagcctgcaa cgggagcttc ctcgttggcg gcgtaccatt
     5761 taattcccct gaatccgctc tcctcgttcg tgccaaagat gatccttatc ctccttgata
     5821 gccgatggtc ctcatcagag aggatcttga gggcccagag ggccgctatg gtcggcccct
     5881 tgtcatcgat ggttccccgt ccgaacatct tcccgtcatg gatctcagcc ccaaaggggg
     5941 ggtaagtcca ccctttccct tcagggacta tgtcaagatg accgagaacg gcaaccattt
     6001 ccttaccgga gccgtactcg gcgtatccta tcatcccgtc atggttgacg gtccggaaac
     6061 cgagttcgga ggccgtctcc agggccaggt tgagaacctg gtggactccg tccccgaagg
     6121 gtttaccctc ctcgggagtt ccttcaacgc tccttatcct taccatatcc tgcaaattcg
     6181 atattatcct gctcttgtac ctttccagaa ggtccctctc agtcatttct tcacaccctt
     6241 cagaggaccc tgagtt
//
LOCUS       DAOTIQ010000085         5810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_16341_length_5810_cov_8.963858, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000085 DAOTIQ010000000
VERSION     DAOTIQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16341_length_5810_cov_8.963858"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(754..4143)
                     /locus_tag="P5541_08540"
     CDS             complement(754..4143)
                     /locus_tag="P5541_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRV72070.1"
                     /translation="MSKTSKVALVILIFASLAAGAALVMASRADIGASTVASEIAGAA
                     RQYLGADLSISSIYGNPLRGFVISDLSISKDGKPIFSSMQASVTFRLLSLITGSPRIK
                     TLSLGGADLDWRKISPLLSFEQTGEPLSLPFDRFEVQKSIVRTVAGDFMIEEARIIPG
                     SSDITMSGSLFFRETPFSLRGSMQLEKERLLLKDLNIRLAGGNISLSGRLTPVVDMAG
                     KIQDLDLEIVEKFWPDLAKQGYSGTFSTTFTAKGLLPDVDIEGKLEIPEGKVYGIDLN
                     KVSSPWTFSDNALQITDLQGSANGTPVSGKLLFAFSSMPPVTTIDLRAGKTDISAWRG
                     SFPWLSIAGGVIDSVEIRLQGLSNALSGPVTLRSSSLTLAKQPLSNVRASMNLEKGSQ
                     VAVNLGGVWLGSQITGKGKIDLTKTPSFDLTFSGKDLDLSKAAGIIPVKNLDLSGKSS
                     GSVRIFGRGQDVQSEGTLWSDLIRAAGESVEKPDIRFAYQKGTITLRSIGARWRGTRI
                     AGSGTISGLAGDKGVFDLSGNVDETPISSLAGFVPALGEFKLEGKASANWSLKGPLKS
                     PMLALEIFSTRLSAPGLAKLNGIKVLTKVALPPASGMPDMRLDMAAESLQFTDFSIDS
                     LRTALSVSAGKITIENGQGKIFGANLAISGSVGLPAGDKPANLDLKGTMSGIDLSALG
                     KASPVSLKGPVEAQVSLKGDLPDPQIALSGKSSSLVLAGLNLADLTFKAQGSPSRITI
                     EELKGSAGKGTFAAKGTFSMLSGGIDMDFSIDGKGLELEKLTKELQGNGAMDFSGAFD
                     ATLAGSLKKGKWNGKGELLSNRFTAYGLVFTNASIPLLLQEEKILAQNSKADFYGGSV
                     TADGSIEIPSKKWNIRGSVSKADIAPAVQAAFPLDGKISGKGDLDITLSGAFGKHLLV
                     TGRGNLVASNGELSGFKALNAIAKTYGLSSIRYRLADVSFKIDGNVITLLPGSRATAH
                     PDDPLYRYFSADGPAGPGGSLNLFCSGLVNVQALNTLLGAIQGLAGAGSASPQALLEG
                     LIGGFVGGMGSQDFRDVSFRVGGTWNNPSMSSFKISSPSSSMQTIPETGGAGQTQNSN
                     QQKVTISVPTGEGADKPSDAEGQIKQQVIEQILRNVIPGSD"
     gene            complement(4379..5158)
                     /locus_tag="P5541_08545"
     CDS             complement(4379..5158)
                     /locus_tag="P5541_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRV72071.1"
                     /translation="MRRSFPPAIFLLLLILLCQDPGQAAMLRVQVLEVFDGDTIRVSM
                     PGGKQETVRYIGIDTPETHHPDRGVEELGKEASKANNELVFGRHVFLETDVEKRDRYG
                     RMLAYVWIEKEGKIYMVNEILLRMGYAMPFTFPPNLKHTDIFREAFARARRENRGLWA
                     RASERRFSPYQLWTFLPSLSGLFVTVEMVVKRVEESERRFTLLPSGGKTTLVIYKSDS
                     PLFGPIGNYSGKRLTVVGKIVPGYAGPEIVIADPVQILDVK"
     gene            5297..>5810
                     /gene="rsmG"
                     /locus_tag="P5541_08550"
     CDS             5297..>5810
                     /gene="rsmG"
                     /locus_tag="P5541_08550"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRV72072.1"
                     /translation="MSLVSHEIDKFGPLLREYASLLSSLNERARITGPSDPSVIMEDH
                     IRDCAWSLALAPEKGSVIDLGAGGGLPGIVWAICRPGLRVTLLESVGKKCRVLEEIVS
                     ALGLSNVEIACTRSETLAQNNREAYGIVVSRAVGHLGIVAEYASPLVETGGFALVFKG
                     PKVLDEIGEVG"
BASE COUNT         1303 a         1549 c         1554 g         1404 t
ORIGIN      
        1 ctcaacgact acagggagtg aatcggaaaa ccttaaaggc gtgaacggtg aacgggaaac
       61 aaaggcggca ggaaacagga gactggaaac tggaagggca aaaaagccgt aagacgttag
      121 tcgtgagacg aatggaaaaa caagggcaag ggccggaggg aaggctttac cgaaggctca
      181 ggagccggtg agtttcgcag ccgtcgcgga ggggcaggaa aagcacacct gccggtaggg
      241 tttttaccgg cactgtgcga tcgccccgca gcaggcggag aaacgaacgc tttcaggcga
      301 gttgcctttc gggaaagact tcactccggg cccggcagta tccctgatgg aatttgaccc
      361 agatttccgc cggtaggcaa agggcgcgta acgtccgcct gctggcggat tccgcttcgc
      421 cccgctcaga atgacaatca cttgcccttg ttcttccgct tcacaccgtt caccatttac
      481 cgttgaccgt ttacgccctt gatttttcgt cttacgtctc acttgccccg cttttttcgt
      541 ctcacttctt acgtctaact tcttacgctc ttgcctttgc acaccgttga ccgtttacgc
      601 cctttctctt gcccttcctg cctcctgtat cccgtctcct gtatcctcgc cttttcgatt
      661 cccgtctcac gtctcacgat cttgccgtag tgtttcacgt ggaacaaaaa aagggggcgg
      721 ccctacgggc cagccccctt atgctttttc gtcctaatcg ctaccgggta ttacgttgcg
      781 aaggatctgt tctattacct gctgttttat ctggccctca gcatcggaag gcttatcggc
      841 gccttctccg gttggaacac tgatggtcac tttctgctgg ttgctgttct gtgtctgtcc
      901 tgcgccgccc gtctcgggga ttgtctgcat cgatgaggaa ggcgatgaga tcttgaaact
      961 ggacatggag gggttattcc aggttccgcc cacccggaaa cttacatccc tgaaatcctg
     1021 gctccccatc cctccgacga aacctcctat aaggccctca agcagagcct gcggtgaggc
     1081 tgagcccgcc ccggccaacc cctgtattgc cccaagaaga gtgttgaggg cctggacgtt
     1141 taccagtccc gagcagaaga ggttcaggct tccgccgggg ccggccggcc cgtcggccga
     1201 gaagtatctg taaagaggat catcggggtg tgccgtggcc cggctcccgg gcaggagcgt
     1261 aatgacattg ccatcaatct tgaaactgac atcagccagc ctgtagcgga tggaggacaa
     1321 accgtaagtc tttgctatgg cgttaagggc cttgaagcca gaaagttccc cattactggc
     1381 gaccaggttc ccccgccccg ttacaaggag atgttttcca aaggcacccg agagggttat
     1441 atccagatca cccttgccgg agatctttcc atccagaggg aaggctgcct ggaccgcggg
     1501 cgcaatgtcg gctttggaaa cagagcccct aatgttccac tttttggaag gtatctcgat
     1561 ggaaccgtct gccgttacag agcccccgta aaaatcggcc tttgaattct gggccaggat
     1621 cttttcttcc tggagcagaa ggggaattga tgcgttggtg aacacaagtc cgtaggcggt
     1681 aaaccggttc gaaagcagtt cacccttgcc gttccatttg ccttttttaa gggatcccgc
     1741 aagagtcgca tcgaaagccc cggagaaatc catagcgccg tttccctgaa gttctttggt
     1801 taatttctca agctccagtc cttttccatc gatggagaaa tccatgtcga tccctccgga
     1861 aagcatggaa aaggtcccct ttgcagcgaa agtacccttt ccggcggagc ccttcagttc
     1921 ctctatggtg atccgggaag gagacccctg ggctttgaag gtcagatcgg ccagattcag
     1981 gcctgccagc accagggaag aagattttcc tgacagggcg atctgggggt cgggaagatc
     2041 ccctttgagt gaaacctgtg cctccaccgg ccccttgagg gacacgggag aagctttgcc
     2101 aagggccgaa agatcgatac ctgacatggt ccctttaaga tcgaggtttg cgggcttgtc
     2161 acctgccggg aggcccaccg aaccggagat ggcgagattg gctccgaata ttttcccctg
     2221 accgttctct atggtgatct ttccggcaga gacgctgaga gccgtcctca ggctgtcgat
     2281 tgaaaagtcg gtaaattgaa gactctctgc tgccatgtcc aggcgcatgt caggcatccc
     2341 cgaagcggga gggagggcca ccttggtcaa aacctttatc ccgttcagtt ttgccaggcc
     2401 gggagcggat aagcgggtgg aaaaaatctc cagggcgagc atgggggatt ttaacgggcc
     2461 cttcaaggac cagtttgccg aggcttttcc ttcgagtttg aactcgccaa gggcaggaac
     2521 aaaacccgcc agtgaagata ttggagtttc atcgacgttt cccgatagat cgaaaacccc
     2581 cttgtcacca gccaggcccg agatagtgcc agaaccggca atcctcgttc ccctccaacg
     2641 ggcccctatg gatcgcaagg ttatggtccc tttctggtat gcgaaccgga tatccggttt
     2701 ttccacgctt tctccggctg cccggataag gtcagaccag agggtcccct cggattgcac
     2761 atcctgaccc ctgccaaaaa ttctcaccga tccggaggat ttgcccgaaa ggtcaagatt
     2821 ttttactggt atgattccgg ctgctttcga gagatcgaga tctttgccgg aaaaagtcag
     2881 atcaaaagag ggcgttttag taagatcgat ctttcctttt cccgttattt gtgatcccag
     2941 ccagaccccc ccaaggttca ccgcgacttg tgaccccttt tcgaggttca ttgaggcccg
     3001 aacgttcgaa aggggctgtt ttgccagggt caggctggat gatctgaggg ttaccggtcc
     3061 ggaaagggcg ttggaaaggc cctgtaatcg tatctctacc gaatcgatca cgcccccggc
     3121 gatcgaaagc cagggaaatg atcctctcca ggctgatatg tcggtttttc cggctctcag
     3181 gtctatggtt gtcacaggag gcatggaact gaaagcaaag aggagtttcc ccgaaacggg
     3241 tgtaccgttc gcacttccct gaagatctgt tatctggagg gcgttgtccg agaatgtcca
     3301 tggggaggaa accttgttca gatcaattcc gtagaccttt ccttcgggga tctccagttt
     3361 tccctcaata tccacatccg gcaaaaggcc cttcgcggta aaggtggtgg agaaggtccc
     3421 cgaataaccc tgctttgcca ggtcgggcca gaacttttcc acgatctcca gatccaggtc
     3481 ctgaatttta ccagccatat ctaccacggg ggtcaaacgc cccgaaaggg aaatgttgcc
     3541 tcccgccagg cgaatattca ggtctttcag aagaagcctt tccttttcca actgcatgga
     3601 tccccggagg gagaagggag tttccctgaa aaagagggaa cccgacatag taatatccga
     3661 gctccccggt atgatccttg cttcttcgat cataaaatct cccgccacag tcctgactat
     3721 gcttttttgg acctcgaagc ggtcaaaggg cagggagaga ggttctcccg tctgttcaaa
     3781 agagagcaag ggagatattt ttctccagtc aagatccgct ccccccaaag acaacgtttt
     3841 aattcggggt gaaccggtga tcagtgagag aagccggaat gtgacggaag cctgcatgga
     3901 agaaaagatc ggtttaccgt ccttgcttat ggaaagatcg gaaataacaa aaccgcgcag
     3961 agggttacca tatattgaag atatggaaag atcggcgccc aggtattgcc tggctgcacc
     4021 ggcaatttcc gatgccaccg tggaggctcc tatgtctgcg cgggaggcca tcaccagggc
     4081 ggctcctgcg gcgagagagg cgaaaatcaa aattacaagg gcgaccttcg atgttttact
     4141 cacctggcca cactccttcc taaacgggtt ttagacccct caaactaaag ataatacatt
     4201 aagagggaaa gatagctaat cggggaaaat acctaaaaat tgaggagggc cgctattcaa
     4261 ctttgtacgt cttcaagtcc ttgggggggt attgggcccg cggttttgtt gaaaagcccc
     4321 ataaggggtt cctgcttgtg tggaaatgtg gatatctctg tggataaatg gctccttttt
     4381 acttcacgtc aagtatctgg acaggatcgg caataacaat ctccgggcct gcataacccg
     4441 gtacaatctt gccaacgacg gtcagcctct tgcctgaata attaccaatg ggtccaaaca
     4501 ggggagagtc gcttttgtaa atgacaaggg tcgttttacc ccctgatggt agaagtgtga
     4561 aacggcgctc cgattcctcg acccttttca cgaccatctc aacggtaacg aagaggccgg
     4621 aaaggcttgg gaggaatgtc cagagctggt aaggagagaa tcgtctttct gacgcccttg
     4681 cccataatcc ccggttttct ctgcgagccc gggcgaaagc ttcccgaaaa atatccgtat
     4741 gcttcaggtt cggcggaaag gtgaagggca tggcgtaacc catccggaga aggatctcgt
     4801 ttaccatata aattttccct tccttttcta tccacacgta ggccagcatt cttccgtaac
     4861 ggtccctttt ttccacatcc gtctcgagaa agacatgtct gccaaaaacc agttcattgt
     4921 ttgcttttga agcttctttt cccagttcct cgacccccct gtcgggatgg tgggtctccg
     4981 gtgtatctat cccgatatac cttaccgtct cctgtttgcc tccgggcatg gacaccctga
     5041 tggtatcccc gtcgaatact tccagtacct ggacgcggag catggctgcc tggccgggat
     5101 cttggcacag gagaataagt aacagcagaa aaatggcagg gggaaaagat cgcctcacgc
     5161 tttttggggc atctccttcc ggtctgtttt ccctttttga aaagtttatc actcgcgcta
     5221 ccgggagtca caccacctgt tttcaggtat gatatgtgga tgatacaggc cgatattttt
     5281 tcctgggagg ttcattttgt cactggtcag ccatgagatc gataaatttg gccctcttct
     5341 ccgggaatat gcgtcccttc tttcctctct caatgagagg gcccgtatca ctggtccttc
     5401 agacccatcg gtcattatgg aagaccatat tcgggattgt gcctggtccc tggcacttgc
     5461 ccccgaaaag ggaagtgtta tcgacctggg agccggaggg ggtttgcccg gaatagtctg
     5521 ggcgatctgc aggcctggcc tcagagtaac ccttctggag agtgtgggaa agaaatgccg
     5581 cgttcttgag gagattgtgt cagcactggg gttatccaac gtcgagatag cctgcaccag
     5641 gtccgaaacc ctggcacaaa ataatagaga ggcttacggt atcgttgtct caagggcggt
     5701 aggccatctg ggaatcgttg ccgaatatgc ttcccccctc gtcgagactg gtggttttgc
     5761 gcttgttttc aaagggccaa aggttttaga cgagatcggg gaggttggcg
//
LOCUS       DAOTIQ010000086         5791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_16411_length_5791_cov_4.865586, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000086 DAOTIQ010000000
VERSION     DAOTIQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16411_length_5791_cov_4.865586"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="P5541_08555"
     CDS             <1..209
                     /locus_tag="P5541_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HRV72073.1"
                     /translation="MLSVAHGKWLGHSPEEERAMVAAMAEAGAVDGVTGLRELSVDGL
                     EISEHMEVVGKIKGVFNKGVNGQR"
     gene            378..746
                     /locus_tag="P5541_08560"
     CDS             378..746
                     /locus_tag="P5541_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRV72074.1"
                     /translation="MGISERPHYNLEVWKQSMELARTIYRKTKIFPVDERFGLVGQMR
                     RSAVSVPSNIAEGACRGSRKDFIRFLMIARGSLGEIETQSYLAKDLGYLENNDDIISL
                     TNRVFRLICGLISSLNTAKS"
     assembly_gap    876..975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1102..1596)
                     /locus_tag="P5541_08565"
     CDS             complement(1102..1596)
                     /locus_tag="P5541_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRV72075.1"
                     /translation="MDKKTAKRVALGAVMAATVAVATMLHIPLPGLRIYFNLGEGVIY
                     TIAILFGARYGAACGGIGAAMADIILGYPLWAPLTLLIKGTEGYVVGNLAPSGRLKAI
                     AAGALVMIAGYTASAGILYGWKVAPVEFMTDIAQTGLGAAFALIFAPVLEKRIQYKGV
                     NGER"
     gene            1746..2180
                     /locus_tag="P5541_08570"
     CDS             1746..2180
                     /locus_tag="P5541_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72076.1"
                     /translation="MQKTGRFFLLVVLLLSVPLVALLFWQQGHSFSAGPVTVTGAVVC
                     RELDDSGSPVGEGPLFRWGGRQVCLLFKYEAPRPGGTLGVTWRFQGQEIFRETIRVND
                     KGGVRAFFLLREDGSPLPVGDYEVSIEGGGRKSYGVPFSVRR"
     gene            2223..2882
                     /gene="fsa"
                     /locus_tag="P5541_08575"
     CDS             2223..2882
                     /gene="fsa"
                     /locus_tag="P5541_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRV72077.1"
                     /translation="MRFFLDSADIEEIRKAARWGVIDGVTTNPSLVAKSGGGDFHALI
                     GEICRIVEGPVSAEAVSLDAEGMISEGRELSGIAPNVAVKIPMCPEGMAATSVLSREG
                     IDVNVTLVFTPQQALLAASAGAAFVSPFVGRLDDIGEDGIGLVSDIAEVFDIHGIDTE
                     IISASIRHPRHVIDSALAGADIATVPLSILEKCFDHPLTESGIARFLKDWEGYRKNHG
                     R"
     gene            2872..4239
                     /gene="rho"
                     /locus_tag="P5541_08580"
     CDS             2872..4239
                     /gene="rho"
                     /locus_tag="P5541_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRV72078.1"
                     /translation="MEDNGMTETRQLEETPPPNDTPANNGNGETKKVPRPKHSFADLS
                     GRTLADLRKYAKDLGVAGISALRKDDLVIAILKEQTGQMGFRFGGGTLEVLAEGYGFL
                     RPRGLLPSDHDIYVSASQIRRFGLRNGDVVWGLIRPPKDQEHYEALLRVEVVNYSDPE
                     EARKRPHFEKLTPIFPDQRLVLETKRTELTARVIDIFSPIGKGQRALIVSPPKAGKTT
                     VLKNIANAVTTNQPDVILMVLLVDERPEEVTDMARSVDGEVIASTFDRPAEEHMRVAN
                     LALEKAKRLVEVGKDVVLLLDSITRLARASNLIVPPSGKTLSGGMDPAALYFPKRFFG
                     AARNIEEGGSLTIIGTALVDTGSRMDEVIYEEFKGTGNMEVHLSRKLSEQRIFPAIDI
                     TRSGTRREELLMDAEELQRVWVLRRRITNVDEAEVLNLILGKLRSSATNSEFLATIKV
                     SQGKE"
     gene            4257..5591
                     /locus_tag="P5541_08585"
     CDS             4257..5591
                     /locus_tag="P5541_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRV72079.1"
                     /translation="MRTPKKRIARLSGRIGFWTAIITMISAGLFLSACALFTSGINDP
                     SISGGAARASTVPDMPSGFIVVDVSRSLLADATATGAVAMGIGPIPEGPSLFEEDLVL
                     EEPSDEDEEEPAEAPKEWTEYFVKPGETLSDIAASFGVPAKVIQDANRLENPDRLSEG
                     QELLIPVDASAVKAAVSELDRRKAADASSVQGDRAIGVQKYTVREGDSLWSIANSFNL
                     DINTLFGCNTMKDPNYLRVGTELRIPNQDGIFYKVAKGDTLDSIAKKHGSQAGSIQKA
                     NDFGSKTLQAGAEIFIPGAKPVTTVYALLGDPSYKGTQKFSKSFAWPLVGGITSGYGW
                     RRHPFTKRRDFHTGIDIKGPTGRLIRAAKSGRVVYSGWMGGYGRVVVIDHGKGYTTLY
                     AHCRSLLVSKGQRVSVGQPVGKVGSSGQATGPHLHFEVRLNNKPINPMQVLK"
BASE COUNT         1274 a         1491 c         1633 g         1293 t
ORIGIN      
        1 gcatgctctc agttgctcat ggcaaatggc ttggccactc accggaagag gagcgtgcaa
       61 tggttgccgc catggccgag gcaggggccg ttgatggggt aacggggctg agggaactgt
      121 cggtggatgg attggaaatt tccgaacaca tggaggtagt tgggaagata aagggagtat
      181 ttaataaagg cgtaaacggt caacggtaaa cggtgaacgg tgaacggcaa gagccttaaa
      241 ttcaactgcg tgaacggtaa acggtgaacg gtgaacggga agggcaagag ccttaaattc
      301 aactgcgtga aggcgtaaac ggtcaacgga aaaaacggaa agcaatactt tccccaaact
      361 taggaaagga cgatcttatg ggtatttcgg aacggcccca ttataacctt gaagtgtgga
      421 aacaatctat ggaacttgca cgtaccatct acaggaagac caagatattc cctgtggatg
      481 aaaggtttgg attagtaggt cagatgagac ggagcgcagt ttcagtcccg agcaacattg
      541 cagaaggggc ctgtcggggt tccagaaagg actttatcag atttcttatg atcgcccggg
      601 gatctctcgg ggagatcgag acccagtcat accttgcaaa agatctgggc tatctggaga
      661 ataacgacga tattatcagc ctgacaaacc gggttttccg cctgatatgc ggattgatca
      721 gttctctgaa tacggcaaaa tcctgaaaca tattattttt gggaatggtc tatgttccgg
      781 ttgccgggtt gattttccgt ttaccgttta cgcccttgac ccgatgtttc tggccctggt
      841 tttgtttggt tttaccgttg accgtttacc gttcannnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnngccct tgatccgatg tttctggccc tggttttgtt tggttttacc
     1021 gttgaccgtt taccgttcac cgttcacgcc cttgatccga tgtttctggc cctggttttg
     1081 tttggtttta ccgttgaccg ttcaccgttc accgtttacg cctttatatt gaatcctctt
     1141 ctccaaaacc ggtgcaaaaa tcaacgcgaa ggcggcgccg aggcccgtct gggcgatgtc
     1201 ggtcatgaac tcaacggggg cgaccttcca gccgtagagg atccccgcac tggccgtata
     1261 cccggcgatc attaccaggg ctccggccgc gatggccttt agtctgccac tgggggccag
     1321 gtttcccact acgtaacctt cggtgccttt tatcagcagc gtaaggggtg cccagagagg
     1381 atatcccagg ataatgtcgg ccattgccgc tcctattcct ccgcacgccg cgccgtaacg
     1441 cgctccgaag agtatcgcta tcgtgtagat gactccctcg ccgaggttga aatatatcct
     1501 taaccccgga agaggtatat gaagcatggt tgccacggcc accgtcgcgg ccattaccgc
     1561 ccccagggca accctttttg cggtcttttt gtccatgatt ttttcccaac ctccctgatt
     1621 gctatttgta cacagagtct agacgtgata gagtatttcg ggtatagcca gtcaggcgtg
     1681 aaatcaggct tttttggacc gttttcctca agactattgc attccagcgg ggttcatggg
     1741 ttataatgca gaaaaccggc agattctttc tccttgtagt actccttttg tccgtgcccc
     1801 tggtggcatt gctgttctgg cagcagggtc acagcttctc tgcgggcccg gtgacggtta
     1861 ccggcgccgt tgtttgccgg gaactcgacg attcaggttc tcctgtcggc gaaggacctc
     1921 ttttcagatg gggcggccgt caggtgtgtc ttcttttcaa atatgaagcc ccccgtccag
     1981 ggggtaccct gggcgtaacc tggcgttttc agggccagga gattttccgt gagaccataa
     2041 gagtgaacga taaaggcgga gtgagggcct tcttcctgct cagggaggac ggcagtccct
     2101 tgcccgtggg tgattacgag gtttctatcg aaggcggagg caggaaaagt tacggtgttc
     2161 ccttttccgt cagaaggtag ccttgtcatt tttctaattt ctgaaaggag ttttcctaat
     2221 tcatgcgttt tttccttgac agcgccgata tcgaagagat cagaaaggcc gcacggtggg
     2281 gtgtaataga cggggtcacc acgaaccctt cgctggtggc caaaagcggc ggaggggatt
     2341 tccacgcact cataggggag atttgccgta tagtcgaggg gccagtgagc gccgaggccg
     2401 tttctcttga cgccgagggc atgatctccg aggggaggga actctccggc atcgcgccca
     2461 acgtggccgt caagataccc atgtgtcccg agggtatggc tgccacatcc gtcctttccc
     2521 gtgaagggat cgacgtcaac gtgacgctgg tattcacccc tcagcaggcg cttctcgcgg
     2581 cgtcagccgg agctgccttc gtgagtcctt ttgtcggccg tcttgacgat atcggcgagg
     2641 acggaatagg tctggtctcc gacatagccg aggtattcga catccacggc atagatactg
     2701 agatcatatc cgcgagcatc cggcatccgc gacatgtaat tgattcggct ctcgccgggg
     2761 ccgacatcgc gacggtgcct ttatcaattc ttgaaaagtg tttcgatcac ccgctgacgg
     2821 aaagcgggat agccaggttc ctgaaagact gggagggtta cagaaaaaat catggaagat
     2881 aacggaatga ccgagacccg gcaactggaa gagactcccc cccccaacga tacccccgcc
     2941 aacaacggga acggagagac caaaaaggtc ccgaggccca agcactcctt cgccgatctt
     3001 tccggaagga cccttgcgga tctgcgcaag tacgcaaagg atctgggcgt ggccgggata
     3061 tccgccctca gaaaggatga ccttgtaata gccatcctga aagagcagac cgggcagatg
     3121 ggttttcgct tcggcggcgg cacactggag gttcttgccg aggggtacgg cttcctgaga
     3181 cccaggggcc ttttgccgag tgaccacgat atttacgtat cggcttccca gatcaggcgt
     3241 tttggtttgc gcaacgggga tgtcgtgtgg ggactcattc gtccccccaa ggaccaggag
     3301 cattacgagg cccttctcag ggtggaagta gtcaactact ccgatccgga agaggcaagg
     3361 aaacgccccc acttcgagaa acttactccc attttccccg accagaggct tgtccttgag
     3421 actaaaagaa cggaactgac tgcaagggtc atagacattt tctcgccgat aggaaagggg
     3481 cagagggccc ttatagtttc tccccccaag gcgggaaaga ccacagtcct gaagaacata
     3541 gccaacgcgg ttaccaccaa ccagccggac gtgatcctga tggttctcct cgtggatgaa
     3601 cgtcccgagg aagtaaccga tatggcccgc tccgtcgacg gtgaggtcat cgcctccacc
     3661 ttcgaccgcc cggcagagga gcacatgcgt gtcgcgaacc ttgccctgga gaaggccaag
     3721 cgcctcgtcg aggtcggtaa ggacgtggtg cttctcctgg attccatcac ccgccttgcc
     3781 cgggcttcga accttatcgt gcccccatcg gggaagacac tctcgggagg catggatcct
     3841 gccgcgcttt atttcccgaa aaggttcttt ggcgcagccc gcaacatcga ggagggcgga
     3901 agcctgacga taatcgggac agcacttgtg gataccggaa gtcgcatgga tgaggtaata
     3961 tacgaggagt tcaagggaac gggcaacatg gaggtccatc tttcgaggaa actctccgaa
     4021 cagaggatct tccccgccat agatatcacg cgctccggca ccaggagaga ggaacttctc
     4081 atggatgccg aggagttgca gagggtctgg gtgctcagaa gaaggatcac gaacgtggat
     4141 gaggccgagg ttctgaacct gatactcggc aagttgagga gttctgccac caacagcgaa
     4201 tttcttgcga cgataaaagt atcgcagggc aaagagtgag gggaggcaac gaactgatga
     4261 gaaccccgaa gaagaggatc gcgaggctct cgggaaggat aggattctgg accgccatta
     4321 tcacgatgat aagcgccggg cttttccttt cggcctgcgc attgtttacc tccgggatta
     4381 atgacccctc catatcggga ggtgccgcca gggcatctac ggtccccgat atgccttctg
     4441 gcttcattgt ggtggacgtc tcgagatcgc ttctggcgga tgccaccgct acaggggcag
     4501 tggcaatggg tataggtccc attccggaag gtccgtccct ttttgaggag gatctggttc
     4561 tcgaggagcc ttccgacgag gacgaggagg aacccgccga ggcaccgaag gagtggaccg
     4621 agtactttgt gaagcccggg gagaccctgt ccgatattgc tgcctctttc ggagttcctg
     4681 ccaaggtgat ccaggatgcc aacaggctcg aaaacccgga caggctctcg gagggtcagg
     4741 agttgctgat tcccgtggat gcctcggcgg tgaaagccgc ggtgtccgaa ttggataggc
     4801 gcaaggcggc ggacgcttcc agcgtacagg gggaccgggc gatcggtgtc cagaaataca
     4861 ccgttcgcga gggcgacagc ctctggtcga tcgccaactc cttcaacctc gacatcaaca
     4921 ccctcttcgg gtgcaacacc atgaaggacc ccaattacct gagggttggg acggaactca
     4981 gaatacccaa ccaggacggt attttctaca aggtcgcaaa gggcgacaca ctggattcca
     5041 tagcgaagaa acacggaagc caggcgggat ccatacagaa ggccaacgat tttggatcaa
     5101 agacccttca ggcgggggca gaaatattta tccccggcgc caaaccggtg accaccgttt
     5161 acgcgctgct tggcgacccc tcttacaagg gcacgcagaa gttctcgaaa tcctttgcct
     5221 ggccacttgt cggcgggata accagcggtt acggctggcg tcgccatcct ttcaccaagc
     5281 gccgtgattt ccataccggt atagacatca agggcccgac gggaaggctt ataagggccg
     5341 ccaagtcggg cagggtcgtc tactccggct ggatgggtgg atacggcagg gtcgtcgtta
     5401 tcgaccacgg caagggctac actaccttgt atgcccactg cagaagtctt cttgtcagca
     5461 aggggcagag ggtctctgta ggtcagcccg taggcaaggt gggttccagc ggacaggcta
     5521 ccggtccaca cctgcacttt gaggtcaggc tcaacaacaa gcccataaat ccgatgcagg
     5581 tgttgaaata gtaccgataa tcgttgattt gccctatgac aaaatgcctt cattgcaata
     5641 aactggacca ggacgaacca gtgtcctggt tcgttttttt tcattcactg tttaccgttc
     5701 accgttgacc gtttacgcct ttgatctttt attttattcc ggaggttttg ccaatatgcc
     5761 caagtacgtt ttcgttacag gaggggttgt t
//
LOCUS       DAOTIQ010000087         5738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_16622_length_5738_cov_5.911490, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000087 DAOTIQ010000000
VERSION     DAOTIQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16622_length_5738_cov_5.911490"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5541_08590"
     CDS             <1..482
                     /locus_tag="P5541_08590"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HRV72080.1"
                     /translation="DNPAGIVEALKQRGFLLDIDRVTEVGKFYKEDLLEIQFLVRALG
                     SAIVTSHMAPALSIRVEGLRDLDVLSFKPMTVEWEDYSIRIPSPEAFVLQKLLIQDYR
                     QSWKREKDIEAVRMVLSRLVKSTPLNMHFNHLSESRKKRIVASAKRSGIILPLSNED"
     regulatory      746..855
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            974..1420
                     /locus_tag="P5541_08595"
     CDS             974..1420
                     /locus_tag="P5541_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72081.1"
                     /translation="MKKEMVYRFHPMNGGVVFAPLYRAQELDRIHRALRDSRTWKELR
                     DSLPWDQYKSIRRYFDDMGMRRPPLECGFSYEDIPGFCEGLYPDWLQAEMEYYLFGKL
                     LEPFGKSVSTGEGSYLHINRDKLPECVRCLEDNGYTVREGSDLIFY"
     assembly_gap    1494..1593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1594..1926
                     /locus_tag="P5541_08600"
     CDS             <1594..1926
                     /locus_tag="P5541_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72082.1"
                     /translation="WDLVDGGLADMARYLNESPLGGTEFQPTGMMIGSSFPSDGKELL
                     NAIGMEIERDPEDEREVFVHDGLTAGMEAPGMPHLFLGCWPSRLVIDPPPKKLFEEEE
                     EDLAHNPG"
     gene            1978..2145
                     /locus_tag="P5541_08605"
     CDS             1978..2145
                     /locus_tag="P5541_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72083.1"
                     /translation="MASSREELEDIPNQLLRNPDCPEYIKEAILKSRQRARQARQAKQ
                     AEDQEEITTRR"
     gene            2343..2471
                     /locus_tag="P5541_08610"
     CDS             2343..2471
                     /locus_tag="P5541_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72084.1"
                     /translation="MIEISRRLLMDEGSGRRLRRFEDVFWKIRQITDMILDNSNLS"
     gene            2650..3645
                     /locus_tag="P5541_08615"
     CDS             2650..3645
                     /locus_tag="P5541_08615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRV72085.1"
                     /translation="MKPTYEELEEKLKRLEGMEKALKEAEQNLLDKIAMWKLMLKQSR
                     EGIVILYPDGSVYEANQRFADMLGYSLEEAYGLSVWDWDAIFEREELQEMLRTVGDSG
                     AHFETRHRRKDGKVIDVELSNNGTVYKGQKLILCICRDVTERNRMQEALIESEKRYQE
                     LSIIDDLTRLYNSRYFYSQLEKEVEISKRYDQPLSLMMIDLDDFKQFNDTYGHIEGDK
                     VLYRMGQILKSSIRKSDTAFRYGGEEFLVLMPVTDIRNASMMAERIREVVCGEIFQPG
                     PGKEVSMTVSIGVAQYQEDIDGRELFRQVDSLMYEAKKEGKNRVCDLSRPGEKKQ"
     gene            3642..5126
                     /locus_tag="P5541_08620"
     CDS             3642..5126
                     /locus_tag="P5541_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV72086.1"
                     /translation="MKAQVVVIGSGGAGLVAAIAAKRSGADVWLVSKTKRGLGTCTAY
                     AGGIFSLVCGDVSSREHFEKTMETGKHLNDSDLVRVLSEEAEGVLRELIRWGVNIKLK
                     NGRASVLSSAPSLIMGGAGFLDELISVAEGSGVKFLDWTVVTSIRVSMGRVTGINIQN
                     WQTGEEGSIDTGSVILATGGGGQIYSHTDNPARITGDGYALALKAGAVLRDMEFVQFY
                     PLGWAEPGFPRWLVDLSLVDSVRVTDSRGDEFLKETLRDWGYKDGKEGNLYARDRSAR
                     LVAQKDREGGVYAHLEDLTEEMLKDPDMQYCLGMDISFFKDIRRPFRLTPLEHYFCGG
                     VVIDSFGRTAVDGLYACGEVTGGVDGANRVGGNALSNIVVFGYRAGITAAKEGRRTTG
                     PGGLPPSGYLHEVSEDNGQDPLALRKELQEETWKALGPIRRTVEMERYLDLLSDFRTR
                     KVRIKNPLGRLLVLEMQGLYETAKAVAQAALKRKESLGTHWRED"
     gene            5453..5722
                     /locus_tag="P5541_08625"
     CDS             5453..5722
                     /locus_tag="P5541_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72087.1"
                     /translation="MSRVLRVFALLSLFGAFAVLRPTVAPALLLPQDIAPYHIQEYWY
                     LPTFYPSVNEAVSALIGIQTKVVQWQGGWVEWTISTPFFRSALRQ"
BASE COUNT         1410 a         1333 c         1670 g         1225 t
ORIGIN      
        1 ccgacaatcc tgcgggaata gtcgaggctc ttaagcagcg agggtttctt ctggatatcg
       61 atagggttac tgaagtgggg aaattctata aagaagatct gctcgagatc cagtttcttg
      121 tccgagctct ggggtcggct attgtgacat ctcacatggc acctgcactt tcgatccgag
      181 tagaaggtct tcgagatctg gacgtactat cattcaaacc tatgaccgtg gagtgggaag
      241 attattcaat ccgaattccc tcgcctgagg ctttcgttct tcagaagctt ttgatccagg
      301 attaccgcca gtcgtggaaa cgggagaagg acatagaagc ggtaagaatg gttctttccc
      361 gtcttgtcaa atcgacccct ttaaatatgc atttcaatca tctctcggag agccggaaga
      421 agcgtatcgt cgccagcgcc aaacgatcag ggatcattct gcctctatcg aatgaagatt
      481 gaaatgttaa cgatcctgga gaaacaggat cgctttgctt ttttaccggt atttcttttg
      541 gatcttatcc gttattgaag ccctggatcg tttttcacaa gcaagtattc cccactcacc
      601 cttcttgtgt tttttccgaa aatagtgaaa acaaacccct tctcatttcc acaaaacaat
      661 tgacaaaacc tgaatattta ccttgtattt aatccttgag aggtatataa tttaaggcag
      721 taacaacgta cctttacaac tgaaactctt atccagagtg gcggagggac tggcccgatg
      781 aagcccggca acctgcggat attttcgcaa ggtgccaaaa ccagcaaacc ccatgagggg
      841 tttggatgat aaggggagaa ggtattccgg agcgtgtatt tccgggatca agcctgttct
      901 tttgcggcct ctccttgcgg aggggccgct tttttttgtg cttttaaaat ccatatcgga
      961 gggagtgtcc gttttgaaga aagaaatggt ttaccgcttt caccccatga acgggggagt
     1021 tgtgttcgcg ccgctttaca gggcgcagga acttgacagg atccacaggg cattgcggga
     1081 ttcaagaaca tggaaggaat tgagggatag cctgccctgg gaccagtaca aaagtattcg
     1141 ccgatacttc gacgatatgg gtatgagaag gccgcctctt gaatgcggct tcagttacga
     1201 ggatatcccg ggcttctgtg agggccttta ccccgactgg ctacaggcag agatggagta
     1261 ttaccttttt ggaaaacttc ttgagccttt cgggaagagc gtgagcaccg gagaagggtc
     1321 ctacctgcat ataaaccggg acaagcttcc ggagtgcgtc agatgcctgg aagataacgg
     1381 ctacaccgta agggaaggtt cggacctgat cttttactga tgacagaatc atattttcca
     1441 tgccgaaggg aaggaagtgt tgttgaattg acgaccataa aggaccagat ccannnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgggatc ttgtcgatgg agggcttgca
     1621 gatatggccc gctatcttaa cgaaagcccc cttggcggca cggaattcca gccgacgggg
     1681 atgatgatcg ggtcaagttt cccctccgac gggaaagaac tgctcaacgc tatcgggatg
     1741 gaaattgagc gggacccgga ggatgagaga gaggtatttg tccatgacgg ccttacggcg
     1801 gggatggagg cccccggtat gcctcacctt ttcctgggct gctggccttc caggctggtt
     1861 atcgacccgc ccccgaagaa actgttcgag gaggaagaag aggatctggc ccataacccg
     1921 ggatagtcag aaagggctgc aattctgggc atcttgtgtt gaaaggaggt gacgttaatg
     1981 gcatccagca gggaggaact tgaggatatc ccgaaccagc tgctgcggaa ccccgattgc
     2041 ccggaatata taaaggaggc gatcctcaag tcgagacaga gggcacggca ggcacggcag
     2101 gcaaagcagg cggaggatca ggaggagatc acaacaagaa ggtgaaaaga agcaggtcct
     2161 ggatgcgggg accaacgaga caccggagga aactgccagg cacctgactt gccccggcag
     2221 tttcctccgg gataacgaag ccgacccccc aagaggcatg tctttatcac cgggagacct
     2281 ggtagaggaa gtaatgcaga caaacggatt agcaagagaa ggaccggaga gcttcctccc
     2341 tgatgatcga gatcagcagg cggctgctga tggacgaagg atcaggccga aggctacgga
     2401 gattcgaaga tgttttctgg aagatacgtc agataactga tatgatactt gataacagca
     2461 acttgtcttg atacggggga tgatcgagag ctcttgtaat agtgggagaa gcagttttac
     2521 ggttgaaaaa attgtggaag gtccgacaat tgaactgatg aactgagtat agattttacc
     2581 ctgaagaaga ggcacgaggc acaaaagaag ccttttatag atatggtctt tcccggaggg
     2641 tgaagatcaa tgaaaccgac ctatgaggaa ctggaagaga agctcaaaag actagaaggc
     2701 atggaaaagg ccttaaagga ggcggagcag aacctccttg acaagatcgc catgtggaag
     2761 ctcatgctca agcagtcccg ggaggggatc gtcatcctct atcctgacgg cagtgtctac
     2821 gaagcgaacc agcgctttgc cgatatgctc ggctactccc tggaggaagc ctacggcctt
     2881 tccgtctggg actgggacgc tatatttgaa agggaggagc tccaggagat gctcaggacg
     2941 gttggcgact ccggagccca ttttgagacc agacaccgca gaaaagacgg aaaggtgatc
     3001 gatgtggagc tcagcaacaa cgggaccgtc tacaagggac agaagctgat cctttgcata
     3061 tgcagggacg taactgagcg caacaggatg caggaggcgc tgatagagag cgaaaagaga
     3121 taccaggagc tgagtatcat tgacgacctt acccggcttt acaactccag gtatttctac
     3181 tcccagctgg aaaaagaggt cgagatctcg aagagatacg accagcctct ttcgctgatg
     3241 atgatcgacc tggacgactt caagcaattc aacgatactt acggccatat cgaaggggac
     3301 aaggtcctct acaggatggg gcagatcctg aaaagcagca tcaggaagag cgatacggcc
     3361 ttccgctacg gtggggagga gttcctggta ctgatgcccg tcacggacat caggaatgct
     3421 tcaatgatgg cagagaggat cagggaagta gtctgcggtg agatcttcca gcccggtccc
     3481 gggaaggagg tctccatgac ggtgagcatt ggcgtggccc aataccagga ggatattgac
     3541 gggagggaac tcttccgcca ggtcgacagc cttatgtacg aggccaagaa agagggaaag
     3601 aacagggtct gcgatctatc acgcccaggg gagaagaaac agtgaaagca caggtcgtgg
     3661 tgatcgggtc gggcggggca ggtcttgtgg ccgccattgc ggcaaaaaga tccggagcgg
     3721 acgtgtggct tgtctccaag acaaaacggg gtctgggtac ttgcaccgcc tacgccgggg
     3781 gaatattttc ccttgtctgt ggagatgttt cttcacggga gcatttcgag aagaccatgg
     3841 agacaggcaa acacctgaac gacagcgacc ttgtacgggt tctctcggaa gaggccgagg
     3901 gtgtcctccg ggagctcatt cgatggggag taaatataaa actgaaaaat ggccgggctt
     3961 cggttctgtc gtcagctccc agtctgatca tggggggcgc gggttttctg gatgaactga
     4021 tctctgtcgc ggaagggtcg ggagtgaaat tccttgactg gactgtcgtc accagtatcc
     4081 gtgtcagtat gggcagggtc acaggcatca atatccagaa ctggcagacc ggggaggaag
     4141 gatccatcga cacgggttcg gtgatccttg ccaccggcgg gggtgggcag atctacagcc
     4201 atacggacaa tccggccagg atcaccggcg acggctacgc cctggccttg aaggcggggg
     4261 cggttctgag ggacatggag ttcgtgcagt tctaccctct tggatgggcc gaaccgggtt
     4321 tccccaggtg gctcgtcgac ctctccctgg tggatagcgt gagagtcact gactccaggg
     4381 gggatgagtt cctgaaagaa acactgagag actggggcta caaggacggc aaagagggca
     4441 acctctacgc ccgggacagg tccgccaggc ttgtcgcaca aaaggaccgg gaagggggcg
     4501 tttacgccca cctcgaggac ctgacggaag agatgttgaa ggacccggat atgcaatact
     4561 gcctggggat ggacatttca tttttcaaag acatcaggag gcctttccgt ctgaccccgc
     4621 tggagcatta tttctgtgga ggagtggtca tcgacagctt cggaaggaca gccgtagacg
     4681 gtctctacgc ctgcggagaa gttacgggag gtgtcgacgg cgccaacagg gtgggaggga
     4741 acgccttgag caatatcgta gtattcgggt accgggccgg gataacagcg gccaaagagg
     4801 ggagaaggac aaccggtccg ggaggacttc ccccttccgg gtatctccat gaagtttcgg
     4861 aggacaacgg tcaggatcct ttggccctga gaaaggaact gcaggaggag acctggaagg
     4921 ccctgggtcc gatcaggaga accgtggaga tggagcggta tcttgatctt ctctcggact
     4981 tcaggacgag aaaggtccgg atcaagaatc cccttggccg tctgctggtc cttgagatgc
     5041 aggggctgta cgagacggcc aaagccgttg cccaggcggc cctgaagagg aaggagagcc
     5101 tgggaaccca ctggagagag gattgaaagc aacccaccgg gaacgggagg caatgatctt
     5161 tcccctgaat ggtcctctct cctttttgag aggtccggta gatcaaagga tcccggacct
     5221 gctatcgaat tactgaatca gacgggtcct cgggtaagcc tggtcagttc agacggttct
     5281 ttcccggtat cagaattccc cccgatctcc tctctgcctc gtacgcttcc gcaaggaaca
     5341 caacttaagg ggacagagct tcacgatccg aattccatgc tttttctggc gtataatgcg
     5401 caatattgtt tccggagctt atcttgagag ttgcctgagg aggctggtcc cgatgtcaag
     5461 ggtgttgaga gtgttcgctc ttctttccct atttggcgca tttgctgttt taagaccgac
     5521 cgttgctcca gctcttctct tgccgcagga tattgccccc taccatatcc aggaatactg
     5581 gtacctcccg accttttacc cctcagtgaa cgaggcggta agcgcgctga tcggcatcca
     5641 gacgaaagtc gtccaatggc aaggcggatg ggtggaatgg actatctcta ccccgttctt
     5701 caggtccgct ttaagacaat aagggtggct ccccctgt
//
LOCUS       DAOTIQ010000088         5715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_16721_length_5715_cov_6.196113, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000088 DAOTIQ010000000
VERSION     DAOTIQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16721_length_5715_cov_6.196113"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(674..901)
                     /locus_tag="P5541_08630"
     CDS             complement(674..901)
                     /locus_tag="P5541_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72088.1"
                     /translation="MNLKGEETGERKGESKTVRGLRETAHQFGMFLRGVDSSMASSTL
                     DGRSPPKGVRREKRDENNGKGLKSNCVNGQR"
     gene            992..2686
                     /locus_tag="P5541_08635"
     CDS             992..2686
                     /locus_tag="P5541_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRV72089.1"
                     /translation="MQERKSSELNQFRLGDLLVQTGNLSEDDLRSVLDEQKKSRKRLG
                     EILVKRKLLTERQLAEVLSRQLKIPLISLARYRPMGEALRLVPENVARRLELVPLAIL
                     DNNRLMIAMADPLNILAVDEIRILTGMDVEVGIATPTEIFRDLDKFYQVQGSLDDAMV
                     EVVESSGGTVDLDREGKTASADDAPVVKLVNSIMEQAVREMASDIHIEPFEKVTRVRY
                     RVDGSLFDAIDFPRKLHPAVSSRIKIMANIDISEKRRPQDGRIMIKALNRNIDLRVST
                     LPTIYGEKVVLRLLDQSNAMVGLEKLGLDQEDRDVVDEIISTPYGIILVTGPTGSGKS
                     TTLYSVLERLSKPEVNIITVEDPVEYTMTGINQIQVNERAGLTFSEALRSILRQDPDK
                     IMVGEIRDMETAQLAVRAALTGHLVLSTLHTNDAPSASIRLSEMGIAPFLVSSSLLAV
                     IAQRLVRKLCDNCKQEYILPDNVAEDVGLPGGTKAFRPVGCETCRGTGYKGRSGIYEI
                     MKVEEKIQNLILEGAPGHQIEKEARVGGMRTLRESGLRKVKEGITSLEEVLQVTLN"
     gene            2717..3799
                     /locus_tag="P5541_08640"
     CDS             2717..3799
                     /locus_tag="P5541_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRV72090.1"
                     /translation="MAMALQEVLAEALRRNGTDIHLGAGLRPMLRVDGDLVTMSGFDA
                     SSNDDIRSVVGEVLTRKQIEDFEENLEIDFSFSFTGPSGETSRFRGNCFYERGNLCVA
                     LRTIPTKIKTIADLKLPAPIVEVTQKHRGLFLVTGPTGSGKSTTLASLIQEINMTRSC
                     HIVTIEDPIEYMYTSDMAVIHQREVGSDTRSFSEALKRVLRQDPDVILIGEMRDLETI
                     SAAVTAAETGHLVFATLHTQSAAQTVDRIIDVFPSHQQQQIRQQLSTVLIGVCTQQLI
                     PTPGGGRVVATDLMFATPAVRNLIREAKVSQMVSIMQTGSSFGMHTMDQDLGRLVREG
                     AIPFDTAKARAHDPKDLERLVFESSF"
     regulatory      3850..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3991..5202
                     /locus_tag="P5541_08645"
     CDS             3991..5202
                     /locus_tag="P5541_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV72091.1"
                     /translation="MRFKYKARNPKGETVSGFMVADSPDQAAVMVRQRGLVPLSFEST
                     ARGEKEPLMERLRKIGTVPLKDKSVLFRQLATMISAGINLGSALDILVAQTTNRRLAA
                     SVKEVKRLVDSGISLSAAMRTQKVFNVLMISIVRAGEEGGVLDSSLDRLAVFLEKQEA
                     LRSKIVSAVSYPAVVMSFSLLVVYILVTFIVPRFARVFNSMGIELPTVTTLIFNFALW
                     MGEKWYIMVFGFIGFIATIIILNRMKATKPYMDRVKLKLPIVGDIVYKAIMARSNRTL
                     SALVEAGVPILMSLEMTSEVAGNYVVQDAYEKMRESARKGQALGETAAKSKVFPLMVA
                     HMITVGEQTGRLEEMLAKIADWFEMELDEKIKRLTAIIEPILIIFVGGIVALVASAIF
                     LPIVGAIQAML"
     gene            5337..>5715
                     /locus_tag="P5541_08650"
     CDS             5337..>5715
                     /locus_tag="P5541_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV72092.1"
                     /translation="MKQWMKKRKSRGFTLVELLIVIVIIGILAGSLLLVMGSGTDKAN
                     ATKIVSDLRTLKAAQLMHYADNDAFATAIGDLSTYLDRTPASDFGTYDATSVDVRITG
                     ITDTKIQTRISDMASESGLSQVTS"
BASE COUNT         1393 a         1431 c         1584 g         1307 t
ORIGIN      
        1 ttaagccctc cagcgccgat ggtactgcgg tctaaaccgt gggagagtag gacgctgccg
       61 gaaacttttc ttttttgtat gagaccgtga gacgtcagac gttaaacgtg agacgaagaa
      121 acgtaaaacg tgagatcaaa agcgtgaaca ttaatatgaa tatacagagc cggaggcgac
      181 aaaagccccc ggctttttgt ttgtccttcc cgttgaccgt tgaccgatta cgctcttgcc
      241 cttgtttgtc atcctgagcg cagcgaagga tctggtttgt tttcgtgaat cgcgaatcgc
      301 gaatcggtaa accctgaggg aggttttacc gaaggctcag gagccggcga gttttgcagc
      361 cgtcgcggag gggcagggaa agcacacctg ccggcgggtt tttcccggca ctgtgcgatc
      421 gtcccgcagc aggcggagaa acgaacgatt tcaggcgagt tgcctttcgg gaaagacctc
      481 ccgatgggca agatctttcg ttagttctgt ggatattatt ttgacccgga ttcccgccgg
      541 tgggcatatg gtgcggaacg tcagcctacc ggcggattcc gcttcgtccc gctggtcctc
      601 agaatgacaa gcccttgcca ttccagtctc ccgactcctg aatcctgact ccttgctttt
      661 ccgcccccac cgttcaccgt tgaccgttga cgcagttgga tttaaggccc ttgccgttgt
      721 tttcgtctcg cttctcacgt ctaaccccct tgggcgggct ccggccgtcc agcgtagaag
      781 atgccataga tgaatccact ccccgcaaaa acatcccgaa ttgatgggcc gtctcccgga
      841 gaccccttac ggtcttgctt tcgcccttcc tttctcctgt ctcctcgcct tttagattca
      901 cgattcactg cctttgctgt atcatagcaa tgtatattaa ttgaggggat aacagaaaac
      961 ctacactgga gccaaatccg aggtgagttg tgtgcaggag cgaaagtcaa gcgaactgaa
     1021 ccagttcagg cttggagacc tgcttgttca gaccggcaat ctcagcgaag acgatctgcg
     1081 gtctgtcctt gacgaacaga agaaatccag aaagagactg ggagagatcc tggtcaaaag
     1141 gaagcttctt acggagaggc agcttgccga ggtcctgagc aggcaattga agatacctct
     1201 gatctccctt gcccgctacc ggccgatggg tgaggctttg cgccttgttc cggaaaatgt
     1261 ggccagacgt ctcgaacttg tgccactggc gatcctcgac aacaacaggc ttatgatcgc
     1321 catggcagac ccactgaaca ttcttgccgt cgacgagatc cgcattctta cgggtatgga
     1381 tgtggaggtc ggaatagcaa ctcccacaga gatattccgt gaccttgaca aattttacca
     1441 ggtccaggga tctcttgatg acgctatggt ggaggttgtc gagtccagtg ggggaaccgt
     1501 ggaccttgat cgtgagggca agaccgcgag cgcagatgat gctccggtcg taaaactggt
     1561 aaattccatt atggagcagg ctgtcaggga aatggcatcg gacattcata tcgagccctt
     1621 tgaaaaagtc acccgggtca ggtatcgtgt tgacggatct cttttcgatg ccatcgattt
     1681 tccgcgcaaa cttcaccccg ctgtatcctc ccgtatcaag atcatggcaa acatagatat
     1741 ttccgaaaaa agaaggcccc aggacggcag gataatgatc aaggccctga acaggaatat
     1801 tgatctccgc gtatcgactc tcccgaccat ttacggggag aaggtcgttc tcaggcttct
     1861 tgaccagtcc aacgccatgg tgggtctgga aaaacttggc ctggaccagg aagacaggga
     1921 tgttgttgac gaaattattt caactcccta cggcataatt cttgtgacag gaccgacggg
     1981 cagcggtaaa tcgacaaccc tttattctgt tctggaacgt ctcagcaaac cggaggtcaa
     2041 tatcatcacc gttgaggatc ccgtggagta caccatgacg gggataaacc agatacaggt
     2101 aaacgaaagg gcgggactga ccttcagcga ggcattgaga tccatactga ggcaggaccc
     2161 cgacaagatc atggtcgggg agataaggga tatggagacg gcccagcttg cggtgagggc
     2221 agcccttaca ggccatctgg tcctttccac ccttcacacc aatgatgctc ccagtgcctc
     2281 tataaggctc agcgagatgg gcatagctcc atttctcgta tcctcatccc ttcttgccgt
     2341 catagcacag aggctggtaa gaaaactttg cgacaactgc aagcaggaat atattcttcc
     2401 cgacaatgtt gccgaagatg tgggccttcc cggaggaaca aaggcatttc gtcctgtggg
     2461 ttgcgaaacc tgcaggggaa caggatacaa gggtcgaagc ggcatttacg agatcatgaa
     2521 ggttgaagag aagatccaga acctcatcct ggaaggcgcc ccggggcatc agatcgagaa
     2581 agaggcgcgg gtcggtggaa tgagaaccct tcgggaatcc ggcctgagaa aagtcaagga
     2641 aggaatcaca agccttgagg aggttctgca ggtcactttg aactgatcat aacggacctt
     2701 gaggggggga aacaaaatgg ccatggcgtt gcaggaggtc cttgcggaag cactcaggag
     2761 gaacggaacg gatattcacc ttggagcggg acttcgcccc atgctcaggg tcgacggaga
     2821 cctggttacc atgtccggct ttgatgcgtc cagcaacgat gacattcgat cggttgttgg
     2881 agaggttttg acaagaaagc agatcgagga tttcgaggag aacctcgaga tcgacttcag
     2941 tttttcattt accgggccct cgggagagac ttcccgtttc agggggaact gtttctacga
     3001 gcgggggaat ctgtgcgtgg ccctgaggac cataccgaca aagataaaga ccatagcgga
     3061 cctgaaactg cctgctccca tagtggaggt cacccagaaa cacagggggc tttttttggt
     3121 taccgggccg acgggcagcg gtaaaagcac caccctggcc tcgctgatac aggagatcaa
     3181 catgacaagg tcctgtcata ttgtaactat agaggatccg atagagtaca tgtacacctc
     3241 tgatatggct gtaatccacc agagggaggt cggatccgat acgagaagtt tctcagaggc
     3301 tctcaagagg gttctaaggc aggaccctga tgtcatcctc ataggagaga tgcgggatct
     3361 ggaaaccata tcagcggcgg tcactgccgc cgaaacgggg caccttgtct ttgccaccct
     3421 gcacacacag agcgcggccc agaccgtcga caggatcata gacgttttcc catcccacca
     3481 gcaacagcag ataagacagc agttgagtac ggtgctcatc ggggtatgta cccagcaact
     3541 gatccccact cccgggggag gaagggtcgt tgcgacggac cttatgtttg ccacaccggc
     3601 cgtacgcaac ctgatccggg aagccaaggt ctcccagatg gtcagcataa tgcagacggg
     3661 ttcgtccttc ggcatgcata ccatggacca ggatctgggg aggctggtaa gggaaggtgc
     3721 catacccttt gataccgcta aggcaagggc ccacgacccc aaggatctcg aaagactggt
     3781 ctttgagtcc tctttctgac gccggaaggt gacttttcat acttccggtg ttaatattat
     3841 gctattgaac ctgataaagg gcaaaaccgc tgaaaggcgg tgacgcaaag ctgcgggtcc
     3901 taacctttgg agtggaagga tagccgggtt gcaatctgcg ggaccctgcg gcttggcgcg
     3961 gggttctttg ttttttcggg gggaattttc gtgagattca agtacaaggc caggaacccc
     4021 aagggagaga cggtttcggg atttatggtt gccgacagcc ctgaccaggc ggcggtaatg
     4081 gtgcgtcagc gtggtcttgt ccctctgtcc tttgagagta ccgccagggg agaaaaagaa
     4141 cccctgatgg aaaggctacg caagataggg acggttcccc tgaaggacaa atccgtcctt
     4201 tttcgtcaac ttgccaccat gatatccgcc ggtatcaatc ttgggagcgc tctcgatatt
     4261 cttgtcgccc agaccaccaa ccgtcggctg gcggcatcgg tgaaagaggt caagaggctt
     4321 gtcgacagcg gtatttccct gagtgctgcc atgaggaccc agaaggtctt caacgtactg
     4381 atgatatcca ttgtcagggc tggggaagag ggaggcgtcc tggactccag cctcgacagg
     4441 ctggcagtat ttctcgaaaa gcaggaagcc ctccggagca agatagtatc tgccgtcagc
     4501 tacccggctg tagtcatgtc cttctcgctg ctggtcgttt acattctggt cacctttatt
     4561 gttcccagat tcgcccgggt attcaactcc atggggatag aacttcccac ggttacgacc
     4621 cttatcttta actttgctct ctggatgggg gagaagtggt acataatggt ctttggcttt
     4681 atcggattta tcgcgacgat catcatcctc aacaggatga aggcgacgaa accctacatg
     4741 gacagggtca aactcaagct tcccatcgtg ggagacatag tttacaaggc catcatggcc
     4801 aggtcgaacc gaaccctgtc cgcccttgtc gaggcggggg tccccattct gatgtccctg
     4861 gagatgacat cggaggtggc gggaaattac gtcgttcagg atgcctacga aaaaatgagg
     4921 gaatcggccc gaaaaggcca ggcactaggt gagaccgccg caaaaagcaa ggtttttcca
     4981 ctgatggtgg cccacatgat aaccgtgggg gagcaaacag ggcggctgga ggagatgctt
     5041 gcaaagatcg cggactggtt cgagatggaa ctggacgaaa agataaaacg tctgacggca
     5101 ataatcgagc cgatacttat tatttttgtg ggagggatcg tggccctggt ggcttcggcc
     5161 atttttctgc cgatcgtcgg cgcgatacag gccatgctgt aaaaatgaga ataccaggtt
     5221 aaagacttta tgtttatatc tgtacacaga aggggggcaa tgctataatc ctttcaatct
     5281 aggttttact attttgtcgg tttgaaagac aatacttttt cggggaggtt caaaaaatga
     5341 aacagtggat gaaaaagcgc aagagtcgcg gatttaccct cgtggaactt ctgatagtga
     5401 tcgtgatcat cggcatactc gccggctcgc tccttcttgt catgggttcc ggaacagaca
     5461 aggccaacgc gactaagatc gtcagcgacc taaggacttt aaaggccgca cagcttatgc
     5521 actatgcgga taatgacgct tttgccacgg caataggtga tctttcaacc tatcttgaca
     5581 ggacgcccgc atctgatttt ggaacatacg acgcaacttc tgttgacgta agaataacag
     5641 gtatcactga tactaaaatc caaacccgaa tttccgatat ggcttctgaa tcgggtcttt
     5701 cccaggttac gagtg
//
LOCUS       DAOTIQ010000089         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_17473_length_5526_cov_7.239262, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000089 DAOTIQ010000000
VERSION     DAOTIQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17473_length_5526_cov_7.239262"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2911)
                     /locus_tag="P5541_08655"
     CDS             complement(<1..2911)
                     /locus_tag="P5541_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72093.1"
                     /translation="MKMTRLFFVLGGLLLALAVAAFTISFGTEMVREQVTEAVAENLD
                     SRITMGDISGNPLRGYVMRDIAMSTDEKDVFAASSIKAKISILSLLTGSPQLSLLEIK
                     GFDSDVERINRLIAKVEKGEEGARLPFQRVRIMESQFGSPWGTADIADVSLSFNGDLL
                     DSNIDLSFGDIPVKGKLDVSMAPGASAIRKMDLDIGKGNISATGTATPELSMAGTINN
                     LDIALVASFWPKIDPALYRGLFSTDFTAKGTWQDPQISGELDFTGSLISGIPVEKASA
                     RWNYDANRVDVADMDVRMLGFPLEGNLAFVFAPSVPPQLMVDLKGTAADLETLAQVSD
                     RLAGMTGTLDEFTLFLEGPVSSPEGRISFRAEKLGFREYSTTSTDITANLRGGNMNIT
                     GKSIFDGSPVSFGGTIRNFMGNPSVSIQGTLRSLSLSSVQKMAPALKEMDLKGIINSD
                     YRVTGPLSGPVISGKAWSDNMTAGEHSLSGLSSLFNYDSASDLLTFSDLKARWKQANL
                     SGRGSVSEASTSKRKGDLVVMATDLDPAFIASMYPKAAEYRLRGRITLEAAVKGLLTT
                     PNVRVSLLSPDLSVMDNFRFVNLNAGTDIANIRSGIPSDFSLDINADRMTLETISLNS
                     VKIGLDKKSKTLNIRQGIASIGAGSLAAEGKMTIEEPFDRTDLDIILRASNIDLGRLK
                     TGDRKGLPVEGTVTGDITLKGKAGNPAISLNASAPSLTAYGMKADAVKAKLSGNTGKI
                     TIEELSGKIGEGSISVKGDVRPEPLVADLAISGQNIDLAPLLSRLEKLKPYDITGKAG
                     LSFNGHFENGKNSGSGKLTSSSVSFMNMTFTDIVLPLELKGDRLVSSGGTGKLYGGQI
                     TNNGSLDLSKMTFADEANVRDTDIASLLKDAFRMKGTITGKARLDAKISGTLGEPLKY
                     SGNGLLQTGQGMVSGFKAIDLIASIHGTKGLQFESSYVPFDLETGKIILLDGTLVK"
     gene            3625..4197
                     /locus_tag="P5541_08660"
     CDS             3625..4197
                     /locus_tag="P5541_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV72094.1"
                     /translation="MKDFTISISDLEQGKRQAVRKSLHTIHGKSDEGPIVIRPFRSGE
                     LGWVSWRHCVLYREEYGFDDTFEYYLLAGMAQFIHEPGDRGQVWVVDCGGTVVGAISI
                     VEISSETGQLRWFILEPGFRGLGLGKRLMETALAYCRERGMTRVFLWTVSELLAARRL
                     YEINGFEMTETKNHFIWGREITEERWDLEL"
     gene            4255..4809
                     /locus_tag="P5541_08665"
     CDS             4255..4809
                     /locus_tag="P5541_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRV72095.1"
                     /translation="MTVKNAMTAENLRSAYGGESMAHMRYLIWGESAEKEGFTRIAKL
                     FKAVSYSEWVHARNHFTVLDGETGDHPVTAGGVFGLKGTAENLTGGFMGEEFEIEQMY
                     PVYLKVAEFQDEKGAVRSFRWALEAEKIHAMLFHRAHEAALAGKDVDIDSFFICAVCG
                     HTATDEEPDICPICGARKEAYKEF"
     gene            4903..>5526
                     /locus_tag="P5541_08670"
     CDS             4903..>5526
                     /locus_tag="P5541_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV72096.1"
                     /translation="MSFRNQIDPKTWKGIRSIMQLQLVIAFVVAVFIMKQWWFCVLMI
                     ALGGFLSAWRGKIWCGMLCPNGGFIDLLWSRISLKLIPFPRVLNRGRILQGAFFAAMI
                     GYFAWIVWLVNVEKGLPVAYASYAQHGMLFLRFCQVNLALAAIMAVVFEPRSFCAHVC
                     PGGTLGTLTGLATKRSPVVLDSEKCIDCGLCKKVCDLPDRLLEPLLSE"
BASE COUNT         1321 a         1287 c         1565 g         1353 t
ORIGIN      
        1 ccttaaccag ggttccatca agaagtatta ttttccccgt ctccagatca aagggtacgt
       61 atgatgattc gaactgaaga cccttggttc cgtgtatcga agcgatcagg tcgatggcct
      121 tgaagccgga aaccattccc tgacctgtct gaaggagccc gttgccgctg tatttcaaag
      181 gctctcccag ggtaccggaa attttggcgt ccaggcgggc cttgccggta atggtgcctt
      241 tcatcctgaa ggcatctttc agcagtgatg caatgtccgt gtccctgaca ttggcttcgt
      301 cggcgaaggt cattttcgag aggtccagag agccgttgtt tgtgatctgg ccgccgtaga
      361 gttttccggt gcctcccgat gagaccagcc tgtcgccttt gagttcgagg ggcaaaacga
      421 tgtcggtgaa ggtcatattc atgaaactta ccgaagagga tgtgagtttt cctgatccgc
      481 tgttcttgcc attctcgaaa tgtccgttaa acgaaaggcc cgcctttccg gttatgtcgt
      541 agggtttcag tttttcaagc ctggagagca atggagcaag atcgatgttc tggccgctta
      601 ttgcgaggtc agccacgagg ggttcaggcc ttacgtcacc cttgacggaa attgagcctt
      661 caccgatctt tcccgacagt tcttcgatag tgatcttccc ggtgttgccg gaaagttttg
      721 ccttgactgc atctgccttc atgccgtaag ccgtaagtga tggtgcagaa gcattgagag
      781 aaatggccgg gttaccagcc ttgcccttga gggttatgtc gccggtaacg gtcccctcca
      841 cgggcagtcc tttcctgtct ccggttttga gtcttcccag gtcgatattc gaggcccgaa
      901 gaataatatc aaggtccgtc ctgtcaaatg gttcctctat ggtcatttta ccttccgctg
      961 cgagggatcc tgctcctatt gaggcaattc cctgccttat gttcagagtt ttactctttt
     1021 tgtccaaccc gatctttacg gaatttagcg atatagtttc cagggtcatc ctgtcggcgt
     1081 ttatgtcaag gctgaagtca gaagggatcc ctgaccggat gtttgctata tccgttccgg
     1141 cgttcaagtt gacgaaccgg aaattgtcca ttaccgaaag atccggggaa agaaggctga
     1201 ctcttacatt gggagttgtc agaagtcctt tgaccgcagc ttccagggtg atcctgcccc
     1261 gaaggcggta ttctgccgcc ttcgggtaca tgctggcgat aaaggccgga tccagatcag
     1321 tggccataac caccaggtcg ccctttcttt tgctggtcga ggcctcgctg accgagcccc
     1381 tgcccgacag gtttgcctgc ttccatctcg ccttaaggtc cgaaaaggtc agaagatcac
     1441 tggcactgtc gtaattgaac agggaggata aaccggacag tgaatgttct cccgctgtca
     1501 tgttgtcgga ccaggccttg ccggatatta cagggcctga aagaggcccc gtaaccctgt
     1561 aatcggagtt gataatgcct ttcaggtcca tttccttcaa tgccggggcc attttctgaa
     1621 cggaagagag agaaagagat ctcagtgttc cctgaattga aacagaggga tttcccatga
     1681 agtttctgat ggtgccacca aaagaaacgg gggacccgtc aaagatgctc tttcctgtta
     1741 tgttcatgtt gccgccacgc aggttggcgg tgatgtccgt gcttgtcgtc gaatactccc
     1801 tgaagccgag tttctctgcc cggaaagaga tcctgccctc gggggatgaa accggcccct
     1861 caaggaatag ggtgaactcg tccagggtac ccgtcattcc ggcaagcctg tcggaaacct
     1921 gcgcaagggt ttccagatcc gcggcggtcc cctttagatc caccatcaat tgtggaggaa
     1981 ccgatggggc aaagacgaac gcaagattcc cctcgagggg gaaacctagc atcctgacgt
     2041 ccatatcagc tacgtctact ctgttggcgt cgtaattcca ccgggcagag gccttttcga
     2101 ccgggatccc cgagatcagg ctgccggtaa aatcaagttc tcccgatatt tgaggatcct
     2161 gccatgttcc cttcgcggtg aaatccgtag aaaaaagccc cctgtaaagg gccgggtcga
     2221 tttttggcca gaaagaggca accaaggcaa tgtcgagatt gttaatagtg cctgccatgg
     2281 acaattccgg ggttgctgtc cctgttgccg agatgtttcc cttccctatg tccaggtcca
     2341 tttttcttat ggcagatgcg cccggagcca ttgaaacatc cagtttcccc tttacgggaa
     2401 tatcaccgaa agagaggtca atgttcgaat ctaacaggtc accgttgaag gaaagggaaa
     2461 catcggcaat atcagcagtt ccccagggtg aaccaaactg gctttccata atacggaccc
     2521 tttggaaggg gagccgtgca ccctcttccc ccttttcaac tttggcaata agcctgttga
     2581 tcctctcgac gtcggaatca aagcccttaa tttcgaggag agacagctgg ggtgatcccg
     2641 taagcagtga gaggatagat atctttgcct ttatgctgga ggcggcaaaa acgtctttct
     2701 cgtcagtgga catggcaatg tccctcatca cgtatcccct gagagggttc ccggatatat
     2761 cacccatcgt tatccgggaa tcaaggttct cggccacggc ctctgtaacc tgttccctga
     2821 ccatctccgt cccgaaactg atcgtgaaag ctgccaccgc caaagccaga agcagcccgc
     2881 caagaacaaa aaacaatctg gtcattttca ttacatcacc ctcttaacta ttcaggatgg
     2941 tttatacttt tcagtaatat tctaactcaa agaaggacaa tcgactccag tgaatgttga
     3001 acgttaaaaa acgaggcggc aggagactgg aggcaggaaa accaggggcg tgaggagtaa
     3061 gaggtgagat gtgagaggtg agaggtgggg ttagaggtga gatggagagg attgtcattc
     3121 tgaggaccag cgggacgaag aatctggttt ggttacgtga ttcgtgaatc gaagaatcat
     3181 caaggagaca ggtaacaggt aacaggtaac aggagtcagg aaaacctaaa aaggccaatt
     3241 cagattcttc gctgcgctca gaatgacagc aggggcaaaa cgtcaacggt gaacggtgaa
     3301 cggaagggca ggggcaaaaa cgtcaacggt gaacggaagg gcaaaggcaa agaagggccc
     3361 tgagggaggg ctttaccgga ggctcaggag ccggtgagtt tcgcagccgt cgctgagggg
     3421 cagggaaagc atacctgccg tggccttttc acggcactgt gcgatcgtcc cgcagcaggc
     3481 ggagaaacga acgctttcag gcgagttgcc ttacgggaaa gaactcccga cgggcagcag
     3541 gggcaaaacg tcaacggtga acggtgaacg ggatggattg aaggcgtgaa cgagggcacg
     3601 acagagaatg gaggttgtca ggaaatgaaa gattttacaa tatcgatcag tgacctggaa
     3661 cagggaaaaa ggcaggctgt caggaaatcc ctgcatacga tacatggaaa aagtgatgag
     3721 gggcctatcg ttattaggcc cttcaggtcg ggagagctgg gttgggtttc ctggcggcac
     3781 tgtgttcttt acagggaaga atatgggttt gatgatactt tcgaatacta tcttctcgcc
     3841 ggtatggcac agtttattca cgagcctggg gacagggggc aggtctgggt agtagactgc
     3901 ggagggacgg tcgtgggtgc gatatctatt gtcgagattt catcggaaac ggggcagttg
     3961 aggtggttta tccttgaacc agggttcagg gggctaggtc ttggaaaacg acttatggaa
     4021 accgctcttg cttactgcag ggaaagaggt atgaccaggg tgtttttatg gactgtcagt
     4081 gaattgctgg ccgcaaggcg cctttacgag atcaatggct ttgaaatgac ggagacgaag
     4141 aaccacttta tctgggggag agaaataaca gaggaaagat gggatctgga gttgtaaact
     4201 ggtctggata gaagatcctt taatatatcg tcatcaggaa aggaagcgat cgttatgaca
     4261 gtgaagaacg caatgactgc ggagaacctg aggtccgctt acggcgggga aagcatggct
     4321 cacatgaggt acctgatctg gggtgaatct gccgaaaagg agggattcac aaggatcgca
     4381 aaacttttca aggctgtttc atactcggaa tgggttcatg cccggaatca ttttacagtc
     4441 cttgatggcg agactggaga tcatccggtt accgcaggtg gggttttcgg actgaaagga
     4501 actgcggaaa atctcactgg agggttcatg ggcgaggagt tcgagataga acagatgtac
     4561 ccagtctacc ttaaagtcgc cgagtttcag gacgaaaagg gggcagtccg gtctttccgt
     4621 tgggcactgg aggcggagaa aatccacgcc atgctctttc acagggccca cgaagcggca
     4681 ctggcgggaa aagatgttga tatagattcc tttttcatat gtgctgtctg tggtcatacc
     4741 gctacggatg aggagccaga catttgcccg atctgcggag ccaggaaaga ggcctacaag
     4801 gagttttgat tcctgctcag gcgattggca gcaagggttg acataccccc atagggtaat
     4861 gtattctctt ccgcgagatc aatacccgga gggggtttct ttatgagttt caggaaccag
     4921 atagacccaa agacatggaa aggtatcagg agcattatgc agctccaact ggtaatcgca
     4981 ttcgtggttg ctgttttcat catgaaacag tggtggttct gtgttctcat gatagcactg
     5041 ggaggttttc tttctgcctg gagagggaag atatggtgtg ggatgctgtg tcccaacgga
     5101 ggtttcatag atctcctctg gagccggatt tcactgaaat taattccttt ccctcgtgtg
     5161 ctgaacaggg ggcgaatcct tcagggggcg ttctttgctg ccatgatcgg atattttgcc
     5221 tggatagtat ggttggtcaa tgtggaaaaa ggtttgcctg ttgcctatgc ttcctatgcg
     5281 cagcatggaa tgcttttcct gcgcttttgc caggtcaacc ttgctctggc cgcaataatg
     5341 gccgtggtct ttgaaccgag atccttctgt gcccatgtat gccccggcgg gacgctggga
     5401 acgcttacgg gtctcgctac aaaacgtagc cccgtggtac tcgacagtga gaaatgtatt
     5461 gactgtggcc tttgcaaaaa ggtttgcgat ctgcctgaca ggcttctgga acctttactc
     5521 agtgaa
//
LOCUS       DAOTIQ010000090         5294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_18481_length_5294_cov_4.488261, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000090 DAOTIQ010000000
VERSION     DAOTIQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18481_length_5294_cov_4.488261"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..668
                     /locus_tag="P5541_08675"
     CDS             219..668
                     /locus_tag="P5541_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72097.1"
                     /translation="MTRKILIALVFMALLFCPAVVSAGSSDICFSLGDSEEGRYVFLF
                     QKDGFNQYLDRTSMEDRGDHLLATIVAIPEAACNDPGNKERYVGVFGFRKSGEEGAVF
                     SVERYSEDGSLLEYQNTEVENPTWEKIPPGHPVHDLLQSVMQYIQIQ"
     gene            1030..1245
                     /locus_tag="P5541_08680"
     CDS             1030..1245
                     /locus_tag="P5541_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HRV72098.1"
                     /translation="MAEFINASSRLSLRVQIGTNDEGRPILRSLSLRDVSADAVADDV
                     VAVSSAIGALLDHPIVETSKIDTDLVA"
     gene            1279..1494
                     /locus_tag="P5541_08685"
     CDS             1279..1494
                     /locus_tag="P5541_08685"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HRV72099.1"
                     /translation="METKTLRMVFGTAAGDPWSLSLRYPKENLTAQDVETAMQAIIDA
                     DVFASGLTDILAAQIIDRTVTDLIDNG"
     gene            1561..1710
                     /locus_tag="P5541_08690"
     CDS             1561..1710
                     /locus_tag="P5541_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HRV72100.1"
                     /translation="MEDFLASAIQSGFAVAVAAYLLVRMESRLEELTNAIKDLQVVIA
                     NKGGA"
     gene            1713..2135
                     /locus_tag="P5541_08695"
     CDS             1713..2135
                     /locus_tag="P5541_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HRV72101.1"
                     /translation="MRPNDIFVSPHFRLTEFQCRCCGCVKLHPELLVKLEKLRDAWGS
                     PLILTSGFRCDKHNRKVNGAEASLHLMGQAADVVVLDENQREFSEAAEKAGFDQIVTY
                     GKRNFVHLGIFGRDSRITLPVNQHKWEMAVPLHVGAAA"
     gene            2116..2616
                     /locus_tag="P5541_08700"
     CDS             2116..2616
                     /locus_tag="P5541_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV72102.1"
                     /translation="MSERQHEKVLKAFRPLVEATARRYEGKGALYEDLVQEGYLAVLD
                     LYPRCSDKDYLAKYLKDRVPARVRSAARREWRISGRCEEFDPEEYRGAFHPEFPWTLW
                     VAEKLLNERDREIARMVYSGHTQKEIAEKLGLTQQAVSSRLSRIREVLKQEQKKERLS
                     KSGNSS"
     gene            2823..3611
                     /locus_tag="P5541_08705"
     CDS             2823..3611
                     /locus_tag="P5541_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72103.1"
                     /translation="MKKTAFRAVSLVAFFLCLVVLSPVAASAGPKIYWHFTIKAFVDL
                     PDTNGVEWAWVTMVELDKPKAYPAEAEVAQEHGGKLEGTVFAFVQGVAWRSAHRYERD
                     VTCKGLPSKSETFWEESESDSVYAMGQLIDTNPVYSFRFGFTNRRILLPDGTWIDPGS
                     ETNVFVGAINVEGEVHEETRGDFYLQVVNYGDNLEHRRRCGKAWVEQYLTAFRSFHIV
                     MERPAVGEYLFAQTLDGPTSRKHFVYRVDRSTSPEHPFWKQQTM"
     gene            complement(3778..4068)
                     /locus_tag="P5541_08710"
     CDS             complement(3778..4068)
                     /locus_tag="P5541_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRV72104.1"
                     /translation="MKEKLYEYDFLEHIDDAEALRIFLEDAFATNDAGHIAKALGLAA
                     RSKGMTEIAEKAGLSREQLYKSLSGKGNPTLKTLLSVLDAMDFGIKPHRRSA"
     gene            complement(4071..4370)
                     /locus_tag="P5541_08715"
     CDS             complement(4071..4370)
                     /locus_tag="P5541_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV72105.1"
                     /translation="MYRIVQTETYLNWERSLKDRKTRQIIAARLFRVANGLLGDVYPV
                     GNGISELRIHYGPGYRIYFKQHGEQMVILLCGGNKSTQKIDVQRAIEIAAQLEED"
     gene            4827..>5294
                     /locus_tag="P5541_08720"
     CDS             4827..>5294
                     /locus_tag="P5541_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72106.1"
                     /translation="MEGQLPERTFLRPGRPKVLTTYRLKARVGHILGECGDAFGKTIK
                     TALQWLGEKVPLPLPEAAWNVESFVLDEHGQRLECVSVPEKGIWTARFSHPDAGMGDI
                     RPVPGRIWLNDLSVARTEKGIQFGVQITCSSLPERDYPVAFIRPGIIKSLAEAV"
BASE COUNT         1300 a         1284 c         1457 g         1253 t
ORIGIN      
        1 aaataatctg ccaagactgg aatattttta tctggaaaac cgtttccagg aatttccatt
       61 aaacaactca ttttgttgcc tctagctttt ctcgataaat tccttgtcat ttgatccgca
      121 aaacaccatt cctgcgctct tgcgataaag gtttttgtca acaatattct ctagaggtac
      181 aatctcctgt aactaaaacc gggaagcagg agcctggtat gactagaaaa attttgatcg
      241 ctcttgtttt catggcgctt ctcttttgtc ccgccgttgt ttcggcagga agttcggata
      301 tctgtttctc cctgggtgat agcgaggaag ggagatacgt tttcctgttc cagaaggacg
      361 ggttcaacca ataccttgac aggacatcca tggaagacag gggagatcac ctcctggcaa
      421 cgattgtggc aatacctgaa gcggcctgca acgaccccgg gaacaaggaa aggtatgttg
      481 gtgtattcgg attcagaaag agcggagaag aaggagctgt tttcagcgta gagaggtatt
      541 cggaggacgg atccctcctt gagtatcaga atacagaagt tgaaaacccg acctgggaaa
      601 agatccctcc tggccacccc gttcatgatt tattgcaatc ggtgatgcag tacatccaga
      661 tccaataact gggaagcgtt cggatctggc tggtatactc cccccgaatt ttaaagtcaa
      721 tgttaattga gcccttgtgt tctttgccaa ggagccttcc cccgttgcct ttccctgttt
      781 tgcctcatgt ccgccggcat taagcccctg tcatttcggg gaagtacgct ccagcgcgct
      841 gtcaaggacg cccatctggc ttcatcccct atgggccttt ggggcccagg ccccgttcct
      901 gaaatctttc aagaatactc tcaaaactga atatttttgc tttgatacag cgtttgttgt
      961 cgcgggcatt taatgacact ctttatatat ggtgagtttg aaagctgatg cagaaaggag
     1021 gtgaagtcca tggcagagtt tattaatgct tcgagccgtc tttccctcag ggtgcagatc
     1081 ggcaccaacg acgaggggcg tcccattctc aggagtcttt ccctgaggga tgtctccgcc
     1141 gacgcggtag ctgatgacgt tgttgccgtt tcttccgcca tcggggcact gcttgatcat
     1201 ccgatcgtgg agaccagcaa gatagatacc gacctcgtag cctagtgaca acaacgaccc
     1261 ttgaaaggag gtgaagcaat ggagacgaag actcttagaa tggttttcgg cactgcagcc
     1321 ggcgatccct ggtcactttc cctcaggtac cccaaggaga accttaccgc ccaggatgta
     1381 gagacggcga tgcaggccat catcgatgct gacgttttcg cgtcggggct aacggatatc
     1441 ctggccgccc agatcatcga caggactgtc accgatctga tcgataacgg ctagttcggg
     1501 atagattatt tcaaggcggg cgcctcccgg tgcccgctga aagaaaagga ggttttgtga
     1561 atggaggatt tcttggcttc tgccatccag agcgggtttg ccgtggctgt tgcggcctac
     1621 cttctggtga ggatggagag ccgtctggag gaactgacca acgccatcaa ggatctccag
     1681 gtagttattg caaacaaagg aggagcctga aattgcgccc taacgacata tttgtctcac
     1741 cccattttcg ccttacggag ttccagtgcc ggtgctgcgg atgcgtgaaa ctgcacccgg
     1801 aactgttggt gaagctggaa aaactccgcg atgcctgggg aagcccgctg atccttacaa
     1861 gcgggttcag gtgcgacaag cacaacagaa aggtcaacgg agctgaggcc agcctgcacc
     1921 tgatgggaca ggctgcagac gtggtggttc tggatgagaa ccagagagag ttctcggaag
     1981 ccgcagagaa agcgggtttt gaccagatag tgacctacgg taaaaggaat ttcgtccatc
     2041 ttggaatatt cgggagggat tcgcgtatta cccttcccgt gaaccagcat aaatgggaaa
     2101 tggcggtacc attgcatgtc ggagcggcag catgagaagg tgctgaaggc gttcagaccc
     2161 cttgtcgaag ctactgccag gagatacgaa ggcaagggag ctttgtacga ggaccttgtc
     2221 caggagggat acctggcggt tttggatctg tatccgagat gcagcgacaa ggattacctt
     2281 gcaaagtacc tgaaagatcg tgttcccgcc agggtaagga gtgcagcaag aagagagtgg
     2341 aggatcagcg gaaggtgcga ggagttcgat cctgaagagt accggggcgc cttccacccg
     2401 gaattcccct ggaccttgtg ggttgccgaa aagctgctga acgaaagaga ccgcgaaata
     2461 gcccgcatgg tgtattccgg ccatacccag aaggagatag cggagaagct gggacttacc
     2521 cagcaggcag taagctcaag gctgagccgg atccgggagg tcctgaagca ggagcagaaa
     2581 aaggaaagat taagcaaaag cggcaacagt agctgaaagg cctggccagc tccaggaaag
     2641 caggtttgtc aggttcatat atatacaacg acaggggaag ccgaaaggcc tcccctgtcg
     2701 ttatgtccgg gcaaatcaaa aaactggtta agcgatataa tgctaaatag atagaaagct
     2761 cgttctgcct cccggagggg cggtttatga ggtactaatg taatagcagg agggtgtggg
     2821 gaatgaaaaa aactgctttc cgggccgttt ccctggttgc cttctttctc tgcctggttg
     2881 tattgtcgcc agttgccgcc tcggctggcc ccaagatcta ctggcatttc acgatcaagg
     2941 catttgtcga ccttccggat acgaatggcg tggaatgggc ctgggtgacg atggtcgaat
     3001 tagacaagcc caaggcctac ccggcagagg ctgaagttgc ccaggagcac ggggggaagc
     3061 ttgaaggcac ggtttttgcc tttgtgcagg gggttgcttg gcgatccgcc caccggtacg
     3121 aacgggatgt aacctgcaaa gggcttcctt caaagagcga gaccttctgg gaggaaagtg
     3181 aaagcgacag cgtatacgcc atgggccagc tgatcgacac gaacccggtc tattcgttca
     3241 ggttcgggtt cacaaacagg aggatacttc tccccgatgg gacctggata gacccgggaa
     3301 gcgagacaaa tgtctttgta ggggcgatca atgtcgaggg tgaagtgcat gaggaaacca
     3361 ggggagattt ctacttgcag gtcgtcaact acggggacaa tctcgagcat cgccggcgct
     3421 gcggcaaagc ctgggtggag cagtacctga cggctttcag gagttttcat attgttatgg
     3481 agaggccggc tgtcggcgag taccttttcg cccagaccct ggatggcccc acatcgagga
     3541 aacacttcgt ttaccgggtc gatcgctcca cctcaccgga gcaccctttc tggaaacagc
     3601 agacgatgta actgaccgcc cccgggaagc agcatcgcct ctaaatattg ccgtactagg
     3661 cgacggggag gcaacgagga agcaggaaat aatggatacc ccccctgtaa tcctgcacga
     3721 aaattgatga ggtttgagga aaaactatca agaaggataa tttatcagcg atccaggtta
     3781 tgcgctccgg cggtgtggtt taatgccaaa atccattgcg tcgaggacgg aaagaagtgt
     3841 ttttaaagtg gggttgccct tcccgctcag tgatttgtaa agttgttctc tggacagtcc
     3901 ggccttttca gctatctctg tcatgccctt ggatctggct gcaaggccaa gtgctttggc
     3961 aatatggcct gcgtcatttg tagcgaaggc atcttccaga aaaatcctca aggcctctgc
     4021 gtcatcaatg tgctcaagga aatcgtattc atacagtttt tctttcattg tcaatcctcc
     4081 tctagttgtg cggcgatctc tattgctctc tgaacatcta ttttttgtgt ggacttattg
     4141 ccaccgcaaa gcaggatcac catttgctct ccatgttgtt taaaatagat cctgtaacct
     4201 ggtccgtaat ggatcctcaa ctcacttatg ccgttgccta ccgggtatac gtctcccaac
     4261 aatccgtttg caacccggaa caaccgggcg gcaatgatct gtctggtttt cctgtccttc
     4321 agtgatcttt cccaattaag gtaggtttct gtctggacaa ttctgtacat acaggtattg
     4381 tagtttataa actacgagaa gtaaagtcat tccaggatct gtgtgttccc tgatgggtgg
     4441 tgaaatcatg gaactcggaa gcgaaaatta ccccctttga gagatcgtca gggatgaggg
     4501 caaaatgtac cgtaaagaga tagagatctt tgcggagatc ggtggggaga taatccccgc
     4561 caccggaatc aacgatgact gaatgcgtgt cgtccctgct gcagaagagg gcaaacaagg
     4621 ctacggggag gcaaaaaggg aactggaaga aatggtaccc ccttttactc ctacacggag
     4681 attgattggg actgagaaaa aacttgtttc aggatctccc ctaactgatc aacccgcaaa
     4741 actttttgga ttcaggttaa aatacagata atgatcaaca actcccagat ctttttcctt
     4801 gctgatacgc gaaggtggta tcttctatgg aaggacaact gccggaaaga accttcctca
     4861 ggccaggcag gccaaaggtt ttaaccacct acaggttaaa ggcccgggtg gggcatatcc
     4921 tgggggaatg cggggacgcc tttggaaaga ctattaaaac agccctccag tggcttggcg
     4981 aaaaggtgcc acttcctctc cccgaagcgg catggaacgt tgaatccttt gtccttgacg
     5041 aacacggcca gcggctggag tgcgtctctg tacctgaaaa ggggatctgg acggcccgtt
     5101 tttcccaccc cgatgccggg atgggagaca tccgccccgt cccggggagg atctggctta
     5161 acgacctttc cgtggccaga accgaaaagg ggatccaatt cggggtccag atcacttgct
     5221 cttccctgcc cgagagggat tacccggtag cctttattcg ccccgggatc ataaaatccc
     5281 tggccgaggc ggtc
//
LOCUS       DAOTIQ010000091         5256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_18661_length_5256_cov_8.257066, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000091 DAOTIQ010000000
VERSION     DAOTIQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18661_length_5256_cov_8.257066"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1103
                     /locus_tag="P5541_08725"
     CDS             171..1103
                     /locus_tag="P5541_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV72107.1"
                     /translation="MARQLRIEYPGALYHVTSRGNEKKDIFRSIKDRDKFLSYLSSAW
                     ERHGAVFHAYCLMSNHFHLMVETPLGNLSWIMKHINGSYTTYFNVKHKRAGHLLQGRY
                     KAVLVQADAYAAELSRYIHLNPVRAKMVSTPEEYRWSSCSRYLEGTEPSWLSTGLVLG
                     YFGTEDEDRRRNYRDYLYDAIGKESRDPLSDSVASTILGSDDFVRDIREKYLDGKEND
                     RELPALRDLSRGPGVAEIKALSEEAFPENERLARMAGIYLCWRYSGAKLKEIGALYGI
                     SESGVNRACFRFENMMEGEKDLRERLQRLTGKLK"
     gene            1201..1500
                     /locus_tag="P5541_08730"
     CDS             1201..1500
                     /locus_tag="P5541_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV72108.1"
                     /translation="MGLQIRIAQEDIVEFCLRNKIRRLSLFGSVLRDDFTPESDVDVL
                     VEFEPGERVGLRFFAMEQELSDLLGRKVDLNTPGFLSRHFREEVQLQAEVLYDAA"
     gene            1487..1828
                     /locus_tag="P5541_08735"
     CDS             1487..1828
                     /locus_tag="P5541_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRV72109.1"
                     /translation="MMQPKDHTRLKHMMEYAEEAKNLASETTRETLATDRKLELALTR
                     LVEIIGEAAARVSSQTREQYPDIPWIEMIGLRNRLIHGYDAVDLDILWDIIQEDLPSL
                     IQRLRALLEKE"
     gene            complement(1934..2383)
                     /locus_tag="P5541_08740"
     CDS             complement(1934..2383)
                     /locus_tag="P5541_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV72110.1"
                     /translation="MKIVENDPLNTVVLEKMFSDPEDLHLVWPVAHWPFDHGQWEQVL
                     DPAKGAISFLVYEGETLIGHAALDRAEDPDTRMVRFLYIAPEYRSKGAGQRMIALLEG
                     YARERLGAKRLEMKVRSFNNRAMRCYEKCGFTEFFRENTKVMMGKDI"
     gene            2566..2763
                     /locus_tag="P5541_08745"
     CDS             2566..2763
                     /locus_tag="P5541_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HRV72111.1"
                     /translation="MSGTERSRIRPGMRVRIIQKQHQGTGELTEGVVRDVLTKSLTHP
                     HGIKVRLEDGTVGRVKYVKEE"
     gene            2947..3696
                     /locus_tag="P5541_08750"
     CDS             2947..3696
                     /locus_tag="P5541_08750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV72112.1"
                     /translation="MDIPRIFNITESAHRIHNPFTPEKFATLGKVLRLEPGARVLDLG
                     SGSGEMLCTWARDFGIVGTGIDLSRLFSGQAKVRAEELGVADRVEFIHGDAAGYVAGE
                     KVDVAACVGATWIGGGVAGTIELLAKSLRPGGMILIGEPYWLQLPPTEDVAKGCQADS
                     ISDYLMLPELVASFGDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDELAKE
                     VRAQLTSEPERYARYTREYMGWGVFALMARA"
     gene            4091..5236
                     /locus_tag="P5541_08755"
     CDS             4091..5236
                     /locus_tag="P5541_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRV72113.1"
                     /translation="MSNVTNRAGRYILQPSGYKAFLPNPLPPDPPVGMTPKLIQALSK
                     AELALGRLDGSTEVLPNPDLFVLMYVRKEAVLSSQIEGTQASLIDLLEYEAKAKEGKA
                     RDVKEVSNYVMALNYGLDRVVELPLSLRLIEEIHGQLLSGVRGSERNPGQFRRSQNWI
                     GEQGSLLQDAAFVPPPPFEMRQAIGELEKFIHKDFEMPHLIKAGLIHAQFETIHPFLD
                     GNGRMGRLLITFFLCQKGILRRPLLYLSHYFKKYRSQYYDLLQQIRDEGDWESWLFFF
                     LKGVAEVAEEATGTAKRIVRLREEFREKIQENLGKNSGKALKLLETLFNEPFVTVRQI
                     CSITGLSYSSANNLAKELEVLGILEETTGKRRNRIFVFKPYMDVFME"
BASE COUNT         1318 a         1251 c         1463 g         1224 t
ORIGIN      
        1 tccttgtgca aacaagtata ctcgaccttg agcaagagtt caaatcaccg gcaaccttga
       61 agcatgagga agaggggtca gaggatgagg ggtcaaacct acacttttga ctaaatgttt
      121 aagttggggg aagatattga ggagcaattt cccgacagga ggttttcccc ttggccaggc
      181 agttgaggat cgagtacccc ggtgctttgt atcatgtgac atcgcgaggc aatgagaaga
      241 aagatatctt caggagcata aaagaccggg ataagttcct gtcctacctg tcatcggcct
      301 gggagaggca tggtgcggtc tttcacgctt actgtcttat gagcaaccac ttccacctca
      361 tggtggagac ccccctcggg aacctctcat ggatcatgaa gcacatcaac ggatcctaca
      421 ccacctactt caacgtcaag cacaagaggg cggggcacct tttgcagggc cgctacaagg
      481 cggtcctggt gcaggccgat gcctacgcag cagagctttc ccgctacatc cacctgaacc
      541 cggtaagggc gaagatggtc tcaaccccgg aggaataccg ctggagcagc tgcagccggt
      601 acctggaagg cacagagcca tcctggcttt cgacgggcct tgtcctcgga tatttcggga
      661 cggaagatga agaccgccga aggaactacc gggactacct gtatgatgcc ataggtaagg
      721 aaagccggga tcccctgtca gattcagtgg cctcgacgat cctgggcagc gacgattttg
      781 tcagggacat cagggagaag tacctggatg gcaaggaaaa tgacagggaa ctgccggcgc
      841 tgagggacct gtcccgtggt cccggtgttg cagagatcaa ggcgttatca gaggaggcct
      901 tcccggagaa cgagaggctg gcaaggatgg cggggatcta tctctgctgg cgttacagcg
      961 gcgcgaagct caaagagata ggggcactat acggtatttc cgaatcgggc gtgaaccgtg
     1021 cctgctttag atttgagaac atgatggaag gggaaaagga tctaagggaa aggctccaga
     1081 ggctgacagg aaaacttaaa tagtcaaagg tgtaggcctg actccgttgc tttggatttt
     1141 tccaaggtta acaaacattg gatataataa gaaaacagaa gcaaggaggt gacactccct
     1201 atgggtctgc agatcaggat tgcgcaggag gatatcgtag agttctgtct caggaataag
     1261 attcgccgtc tttccctgtt cggatcggtc ctgagggatg attttactcc cgaaagcgac
     1321 gtcgatgttc ttgtcgaatt tgagccaggg gaacgtgtcg gcctcaggtt ttttgctatg
     1381 gaacaggagt tgtcggacct tctcggacgg aaggtcgacc ttaatacgcc gggattcctg
     1441 agcagacact tccgggagga agtccagctc caggctgagg ttctttatga tgcagcctaa
     1501 ggaccacaca agactgaagc atatgatgga gtatgcggaa gaagcgaaaa acctggcaag
     1561 cgagacaaca agggaaacac ttgcgacaga ccggaagctt gagttggccc tgacaaggct
     1621 ggtcgagatt atcggagagg cagctgcaag ggtttcttcc cagacccggg agcaatatcc
     1681 tgatattccg tggatcgaaa tgatcggact cagaaaccgg ctgatccatg gttatgatgc
     1741 tgtcgatctg gatatcctct gggatatcat tcaggaagat ctcccttccc tgatccagag
     1801 attgagagcg cttctggaga aagaatagcc tcccttaata tgctttcttc aggctgcaaa
     1861 tctaatactt gttcagatct acacaattga tgacagacca cgaataacac cagcctgttg
     1921 gaatccatgc tcatcatata tccttgccca tcatcacttt ggtgttttcc ctgaaaaatt
     1981 cggtgaagcc gcatttctcg tagcatctca ttgcgcggtt gttgaagctg cggaccttca
     2041 tctccaatcg ctttgcacca agcctctccc tggcgtaccc ctccagaagg gcgatcatcc
     2101 gttgccccgc tcccttgctc cgatactccg gagcgatgta gaggaaccgg accatccgcg
     2161 tgtcggggtc ttctgcccgg tccagggccg cgtgcccgat gagggtttcc ccctcgtaca
     2221 cgaggaagga gatcgcccct tttgccgggt caagcacctg ttcccattgt ccgtgatcaa
     2281 agggccaatg cgcgacgggc cagacaaggt gaagatcctc cgggtcgctg aacatctttt
     2341 ccagaacgac tgtatttagc gggtcatttt cgacgatctt catatcggtt cagcccttcc
     2401 gcagatactg aagtcctttt ccttgttgat gcccgtccag gttgtatgtt atcaaaatat
     2461 gatgacagag gggcctgcgc ttttgactga actggtaaac caggccgctg aatttgatga
     2521 tagtccttcc ccttccagaa ggttattcaa caaggaggat ttttcgtgtc gggtacggag
     2581 agaagcagga tcagacccgg gatgagggtc aggatcatac agaagcagca ccaggggacg
     2641 ggcgaactga ccgagggcgt tgtcagggat gttttgacaa aaagcctcac gcaccctcat
     2701 gggatcaagg tccgtctcga ggacggtacc gttggcaggg tcaaatacgt gaaggaggag
     2761 tgacgagacg aaccttaggg gtatctgctg ccacacctat tacaccgacg atgcggcgac
     2821 ccaaaagaaa aacgggtcaa gagcctggaa gcgtcagatg tgtcaggccg tacacatttg
     2881 acaaacattc tgtcgagata agtcaggaga gaaacgaaat ttacaatata ggaggagttg
     2941 tcagcagtgg acattccaag gatcttcaac atcaccgaaa gtgctcacag gatccataat
     3001 ccgttcacgc ccgaaaagtt cgccaccctc ggcaaggtgt tgcgcctgga accgggggcc
     3061 cgcgtgctcg accttggcag cggttcgggc gagatgctgt gcacctgggc acgcgatttc
     3121 ggcatcgtcg gcaccggtat cgacttaagc cggttgttct ccgggcaggc gaaagtccgc
     3181 gcagaggagc tcggcgtcgc cgaccgggtc gagttcatcc acggtgatgc tgccggctac
     3241 gtcgccggcg aaaaggtcga tgtggcagcc tgtgtcggtg ccacctggat cgggggaggg
     3301 gtagccggca ccatagagct tctggctaag agcctccgcc ccggagggat gatcctgatc
     3361 ggtgagcctt actggttgca gttgccgccg acggaagacg ttgccaaagg atgccaggcc
     3421 gattcgatct ccgactatct catgcttccg gaacttgtcg cgtccttcgg cgaccttggc
     3481 tacgacgtcg tggaaatggt tctggcagac caggaaggct gggacaggta cgaggcagcc
     3541 aagtggctca ccatgcgccg atggctcgaa gcgaatcccg acgatgaact ggcgaaagag
     3601 gttcgagccc aactaacctc ggaacccgaa cgctacgcca ggtacacccg tgaatacatg
     3661 ggctggggcg tgttcgcgct tatggcgcgt gcatagtagg aggaaggcct gtattttgac
     3721 tgaatggtca agatgggaga aatgggaggc agatctttcc cagtcggcaa ggatgaccgg
     3781 gatctatatt tgccagaggc catgactgtt ggtaattatg ggaacataac aggaaaggtt
     3841 aagcatttgg ggaaagggcc cagcaggcaa gcaatatctg aaatagcaaa aagtgcaggt
     3901 ctgcccctgg ggctttgttc tgtcggggtt tttcagagcg tgtttctttc ttcatttttt
     3961 attaatctat ttctacccgt tattattctt ggttgcaaaa agacctttct cttgactctc
     4021 ttatttcaaa aaatggcgtt ttgcaataat ataagtctat gtttaattcc atattatcgg
     4081 aggcaataat atgtccaacg tgacaaatcg agccggaagg tatattttgc aaccttcagg
     4141 ttataaagcc tttctcccga atcccctgcc cccagacccc cctgttggaa tgacgccaaa
     4201 gctgatccag gccctttcca aagcagaact ggccctcggc cgtcttgacg gctcaacgga
     4261 agtattgcca aatccggatc ttttcgtact catgtatgtc agaaaggaag cagttctttc
     4321 gagtcagatc gaagggacgc aggcatcttt gatagatctg ctcgaatatg aagccaaagc
     4381 gaaggaaggg aaggcccggg atgtaaaaga agtttcgaat tatgttatgg ctctaaatta
     4441 tggactggac cgtgttgtgg aattgccctt gtcgttgagg ttgatagaag agatacatgg
     4501 gcaattgctt agcggggtca gaggttcaga gaggaatccc ggccagttca ggagatctca
     4561 gaattggata ggggagcagg gaagcctgtt acaagatgct gcttttgtgc ctcctcctcc
     4621 atttgagatg agacaggcaa ttggagaact ggagaaattc attcacaaag atttcgagat
     4681 gcctcacttg atcaaagccg gactgatcca tgcacaattc gaaacgatcc accccttcct
     4741 tgatgggaat ggtcgtatgg ggcgcctgct gataaccttc ttcctttgcc aaaaagggat
     4801 cctccgtcgc cccctcctgt atctgtctca ttatttcaag aagtatagat ctcaatatta
     4861 tgatcttctt caacagatcc gcgatgaagg agattgggag agctggcttt tctttttcct
     4921 taaaggtgtt gctgaagtcg cagaggaagc caccggaacg gcgaagcgca tcgtccggct
     4981 ccgggaagaa tttagggaaa agatccagga aaaccttggt aaaaattccg ggaaggcact
     5041 taagttactg gaaaccttat ttaacgaacc attcgtaacg gtccgacaaa tctgtagtat
     5101 cactggtctt tcctacagtt ctgcgaacaa tctggctaaa gagcttgaag tgttggggat
     5161 attggaggaa accacaggga agaggaggaa tagaattttt gtttttaaac cgtatatgga
     5221 tgtcttcatg gaataaaata taagtgggtc cttttg
//
LOCUS       DAOTIQ010000092         5242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_18719_length_5242_cov_7.550029, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000092 DAOTIQ010000000
VERSION     DAOTIQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18719_length_5242_cov_7.550029"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5541_08760"
     CDS             complement(<1..366)
                     /locus_tag="P5541_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV72114.1"
                     /translation="MSFRKIMPAGFMVLVAIVLVLVFSSLPVLPSFAGTTVKVGIYEN
                     KPKVFTDDPGRPAGIFVDIIEAVAASEGWQIEYVRGTWSEGLDRLAAGEIDLMPDVAY
                     TAEREKRFSFHSEPVLSDWF"
     gene            complement(610..1065)
                     /locus_tag="P5541_08765"
     CDS             complement(610..1065)
                     /locus_tag="P5541_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV72115.1"
                     /translation="MRRRDREITDISVMESIIRDSSVCRMGLCDGDRPYVVPMNFGYR
                     DGKVYMHSALEGKKLDIIRKNPDVCLEFETDLEIVRAEEACSFSMKYRSVIARGRASI
                     QEESADKAYGLNVIMEHYTGKEFEFPVQALQKIVVITVDIEEMTGKQHL"
     gene            1266..1853
                     /locus_tag="P5541_08770"
     CDS             1266..1853
                     /locus_tag="P5541_08770"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HRV72116.1"
                     /translation="MTYGLTLLSAAAMFTFLAATKAAAHCDTLEGPVIKTARQALEKG
                     DVAPVLKWIREEDEHEVQKAFELALKVRSLGPDAGELADMSFFETLVRIHRAGEGAPY
                     EGLKPGSIVPPVVGKADTALETGDVSELAERISNHVRKAVEERFHQALEKKVTAEDSP
                     AKGREYVEAYISFVHYVESIHALTMGGGHHGGHHD"
     gene            complement(1929..3164)
                     /locus_tag="P5541_08775"
     CDS             complement(1929..3164)
                     /locus_tag="P5541_08775"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HRV72117.1"
                     /translation="MKLFQLLALDIFANSEIVAGKRGLDKDVTWVNMMEILDSLEQLQ
                     PGELLISTGYGLTEGSPLAADIIGNLNERGLAGIAVQPGYYISEIPSSMIRQADELGF
                     PIIRLQPKITFGKVTRAILDFLTQDRISRMELPISFPLRVLDNRRSGVLEEFFDDLLD
                     GNFHSYNEALTRAGSVGLEISSPYKVAVVDPDIGDAGNRDVSHDLMMETRQLIMTHPL
                     APKNIHSRLKGNHIVLVYLAANTKTEEQTLFRNVASNVAEGVDRGGFFVGVGKTTKNW
                     REFKDSYSQAITALEIGKRLFPSGKVAHYEDLGLYRLLCQIKDRNILREFMNETVMPL
                     FEYDSRHKGVLCGTLKVFLESGNQQETADKLIIHRQTLGYRLRKIAEIVRKDLNSPKD
                     RFDLYVGLLIMDIFDPDNL"
     gene            3374..4564
                     /locus_tag="P5541_08780"
     CDS             3374..4564
                     /locus_tag="P5541_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV72118.1"
                     /translation="MINDFRKELDEREMISLIADLVNTPSYPGIPEQETGVAKLIHDF
                     FVKEGIPSEIYEVVDGRCNVVARLEGHGKGKTLLLTGHTDTVPPYDMEDAFRMKIENE
                     KLIGRGVVDMKGALACMVLSMAAIRRSGTRLKGNIMFAGVIGEEGKSEGTCALLKKGL
                     KADAAIVGEPFGWNIGIGQKGLEWFECRILGKAVHGGKQKEGINAISKAGKLIRALDE
                     ELVPRIESRMHPIIGGSSMNYGMISGGFQPSTVAGECVLQFDRRWIPGEKYEDILREY
                     DDFLERLGQDDPQFQAELKVMDSSLMERGYIHEAMELSLEHPLVLLMDKTIFEATGHR
                     AEKKAFPAWTDAGLLSSYGGIPTVIFGPGSPDSAHTAHEYLETSYLVPFTQIYALMAR
                     DFCQ"
     gene            4707..>5242
                     /locus_tag="P5541_08785"
     CDS             4707..>5242
                     /locus_tag="P5541_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HRV72119.1"
                     /translation="MSSAKAVKKETSRKKFKVPHVYIILLTVVAICAVLTYLVPAGHY
                     ERVAGPGGRMIVDPQSFGYVDQSPVGLFDFLLAVPKGMVEIAEIIFFIFIVGGSFGVV
                     NATGAIEAGIGKITRKMAGKERAIIPIVMILFSLGGAIFGMAEETLPFVPIMVTLAIA
                     LGFDSLTGAGIVLAGAGAG"
BASE COUNT         1295 a         1324 c         1321 g         1302 t
ORIGIN      
        1 gaaccagtcc gacaggaccg gctcagaatg gaaggaaaac ctcttctccc tctccgcggt
       61 ataagccacg tcaggcatca ggtcgatctc tcctgcggca agacggtcga gcccctcgct
      121 ccaggtacca cgcacatact ctatctgcca gccctccgaa gccgcgactg cttcgataat
      181 atccacaaag atcccggcgg gtctgccggg atcatcggta aagaccttgg gcttgttctc
      241 gtaaataccg actttgacgg tcgtcccggc aaaggagggc aagacgggaa gggaagaaaa
      301 aacaaggacc agaacaatgg ccactaaaac cataaacccc gccggcatga tctttctgaa
      361 actcaagaaa atcttccttt ttcttgtcgg gtcatttcga tgacggctat ttcctgacgg
      421 caaactgacc atctcccgat cctgtttgag aagatttgag gcttccggat attctacatt
      481 ccctcattac tttgcacaag aaaagagatg atgaaaaaaa tcgattaatt ttgccttcaa
      541 catcatagat ccgtcttttc cccttgcgct tcgtccgttc ccggcactct ccagactctc
      601 ccgttctcct tataaatgct gttttccggt catttcctcg atgtcaacag ttatgaccac
      661 gatcttttga agggcctgaa cagggaattc gaactccttt ccagtatagt gctccatgat
      721 aacgttcagt ccgtaggcct tatcagccga ttcctcctgg atggatgccc tgcccctggc
      781 gatgacgctc ctgtacttca ttgaaaagct gcatgcctct tccgcccgga ctatttccag
      841 atccgtttcg aactccaggc agacatcggg atttttcctg atgatgtcca gttttttacc
      901 ttccagtgcc gaatgcatgt atacctttcc gtctcgataa ccgaagttca tgggaaccac
      961 gtagggcctg tctccgtcac acagtcccat cctgcatacc gaggaatctc tgatgataga
     1021 ctccatgacg gaaatgtcgg tgatctctct gtcccttctt cgcattccat ttcccccttt
     1081 tctcaggaaa ataagtttct gcaaagtatt acgccttgtt acttactatg ataaggcatt
     1141 tcacaggaag gctccaattg agcaaaccga cggagaaaga tatacttggc ctgtcatccg
     1201 ggatttcaag aatctgccct gtaatgggcc tttcatcctg acactgagag aaggagctga
     1261 tggtcatgac ttacggattg acccttctat cggcagcggc aatgtttacc tttttggctg
     1321 caacaaaggc tgcagcacat tgcgataccc tggagggccc cgttataaag acagccagac
     1381 aggccctgga gaaaggggac gttgcaccgg tccttaaatg gatcagggaa gaggacgagc
     1441 atgaggtaca aaaggccttt gaacttgcac tgaaggtccg ctccctcgga cccgatgccg
     1501 gggaactcgc cgacatgtcc ttctttgaaa cccttgtaag gatccatcgg gccggtgagg
     1561 gcgcccccta tgaaggacta aagcccggga gcattgtccc cccggttgtc ggaaaggcag
     1621 acacagccct ggagaccggc gatgtctctg aactggccga aaggatctcg aaccatgtac
     1681 gaaaggctgt tgaggaaagg tttcatcagg cccttgaaaa aaaggttact gccgaagatt
     1741 cacctgcaaa ggggagggag tacgtggagg cgtatatctc tttcgtccac tacgtcgaga
     1801 gtatccacgc cctgaccatg ggcggcggcc atcacggggg tcatcatgac tgagttaaac
     1861 cctgccaggt caaggcacgg ccgggtcggt tgcccccgtg ccttgttttt tgggaccatt
     1921 ggaagaaact aaaggttatc gggatcgaaa atatccatta tcagcaatcc cacgtaaagg
     1981 tcgaaacggt ccttgggaga gttcaggtct ttcctgacta tttctgctat cttcctaagc
     2041 cggtagccca atgtctgcct gtggatgata agcttgtcgg cggtttcctg ctgattcccg
     2101 ctttcgagga aaaccttaag ggttccgcaa agtaccccct tgtgtcttga atcgtattcg
     2161 aacaggggca tgacggtctc attcatgaat tccctgagga tatttcgatc ctttatctgg
     2221 caaaggagac gataaagccc cagatcctca taatgggcca ctttccctga agggaaaagc
     2281 ctttttccta tctcaagagc agttattgcc tgggaataac tgtccttgaa ctcacgccag
     2341 tttttggtcg ttttccctac cccgacgaag aaccctcccc ggtccacccc ttcggcaacg
     2401 ttgcttgcga cattccggaa tagcgtttgt tcctcggttt tggtatttgc agccaggtag
     2461 acgagaacaa tatggttccc cttcaggcgg ctgtgaatgt tcttgggggc aagggggtgt
     2521 gtcatgataa gttgcctggt ttccatcatc agatcatggg agacatccct gttcccggca
     2581 tccccgatat ccgggtccac aacggccact ttataggggg aggatatctc gaggccgaca
     2641 gaaccggctc gggtgagggc ctcattgtaa gaatggaaat tcccgtccag cagatcatca
     2701 aaaaactctt ccagaactcc tgatcttcta ttgtccagga ccctgagggg gaatgaaatt
     2761 ggcaactcca ttctgcttat cctgtcctgg gtcaggaaat ccaggatagc tctggtaact
     2821 ttcccgaaag ttatcttcgg ctggagccgt atgatgggaa aaccgagctc gtcggcttgc
     2881 cttatcatgc ttgagggtat ctcggaaatg taatacccgg gctggacagc tatcccggcg
     2941 agccctcttt cattcaggtt gccaataatg tctgcggcta gtggtgagcc ttctgtcaga
     3001 ccgtaaccgg tagaaataag aagttccccc ggctgcaatt gctctagaga gtcaagtatc
     3061 tccatcatgt tgacccaggt aacatccttg tcgagccctc ttttcccggc cactatttcc
     3121 gaattcgcaa aaatatcaag ggcaagaagc tgaaaaagtt tcatgtcaga tcaccagaat
     3181 tctcctgttt cgtttcagat tcgtcttgca ttttatgtta cgtattatac ctgttgtata
     3241 aaacttggtg gataaattta tttgctgcaa ctaattgctt tttcttacta acaccacgac
     3301 actacaagaa acttcctccg ttcatttaat aatgtttcct tatcttttca atatctttta
     3361 aggagttgtt gttatgatca acgattttcg gaaagaactg gacgaaaggg aaatgatctc
     3421 gctgatcgcg gatctggtta atactcccag ctatcctggc atcccggaac aggaaaccgg
     3481 ggttgcaaaa ctgatccacg atttttttgt caaggaaggg atcccatcag aaatatacga
     3541 ggtggttgat gggagatgca atgttgtagc taggctcgaa ggacacggga aaggcaaaac
     3601 acttcttctt acgggccata ccgatactgt tccgccctat gatatggaag atgccttccg
     3661 catgaagatc gaaaacgaaa agttgatagg ccgtggcgtt gtggatatga aaggggccct
     3721 tgcctgtatg gtgctctcca tggccgcgat caggaggtct ggcaccaggt tgaagggtaa
     3781 cataatgttt gcgggcgtaa taggcgagga gggaaagagc gaagggacct gcgccctgtt
     3841 gaagaagggc ctaaaagctg atgccgccat tgtcggagaa cccttcggct ggaacatcgg
     3901 cattggccag aagggcctgg aatggtttga atgcaggatc ctggggaagg ctgttcatgg
     3961 cggaaagcag aaggaaggta tcaatgccat ctccaaggcg ggaaaactta tcagagccct
     4021 tgacgaagaa ctcgtcccca ggatagagag ccgcatgcac ccgatcattg gaggatcttc
     4081 catgaattac ggaatgatca gtggaggctt ccagccaagt accgtagccg gtgaatgcgt
     4141 tttgcagttc gaccggcgct ggatcccggg ggaaaaatac gaggacattc tcagagagta
     4201 cgatgacttt ctcgaaaggc tagggcaaga tgatccgcaa ttccaggcag aactgaaagt
     4261 aatggattcc agcctgatgg aaaggggcta tatccatgaa gcgatggaat tatctcttga
     4321 acaccccctg gttctattga tggacaaaac tatttttgaa gcaacaggac acagggctga
     4381 aaagaaagca ttccctgcat ggaccgacgc aggacttttg tccagctacg gggggatccc
     4441 gacagtcatc tttggtcccg gcagtcctga cagcgctcac acggcccacg aataccttga
     4501 gacatcatac ctggtcccct tcacgcaaat atatgcgctg atggccaggg acttctgtca
     4561 gtgaaccgca tgatcccccc gtctcatctc atgagaacgt cctgacgttt tttgatgatc
     4621 cctttaaaag gagtgatgcc atgatatcag gttaaaagac cggatcggac atattgcagg
     4681 gcaacattaa aaggagggtc gacaatatga gcagtgccaa ggcggtaaaa aaagaaacct
     4741 ccaggaaaaa gtttaaagtc cctcacgtat atatcatcct tctgaccgta gtagccatct
     4801 gtgctgtcct gacctacctt gttccggcag gacattacga aagggttgcc ggtcctggag
     4861 gcaggatgat agttgacccc cagagtttcg gctacgtgga tcagtctccc gtagggcttt
     4921 ttgactttct tctggcagtc cccaagggga tggtggagat cgcagagatc atattcttca
     4981 tcttcatcgt gggaggttcg ttcggggtcg taaacgcgac cggagcaata gaggctggca
     5041 tcgggaagat cacaaggaag atggccggca aagagcgggc aataataccc atagtcatga
     5101 tcctcttctc gctcgggggc gctatattcg ggatggccga ggaaacgctt cccttcgtac
     5161 cgatcatggt tacactagcg atcgcccttg gctttgattc cctgaccggc gcaggcatcg
     5221 tccttgctgg agcaggcgca gg
//
LOCUS       DAOTIQ010000093         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_21809_length_4674_cov_4.314137, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000093 DAOTIQ010000000
VERSION     DAOTIQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21809_length_4674_cov_4.314137"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="P5541_08790"
     CDS             complement(<1..844)
                     /locus_tag="P5541_08790"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRV72120.1"
                     /translation="MRVAIAGCGALGSVIAFHLLGSGYPGLELELIQRPGEHFDAISK
                     KGLKIHLPDGGTKTLPIPVSPGGTGRGSFDLVIVLVKSGDTGRVAPMIEKELAPMGVA
                     LTLQNGLGNAEELARMLGASRVAAGVTTFGGYRKAPGEVVVAGEGTVIFGPWEKGMDL
                     GKIRDLFLSADLNPQLVEDPREAICRKLVLNAAINPLAAIVRQNNGAIIGNPDLAAVM
                     DLLLGEAVEAMALKGVLIDLEEIRKTIMETLKKTSSNRASMLQDIEAGRRTEIDNING
                     QILAA"
     gene            complement(841..1686)
                     /gene="panB"
                     /locus_tag="P5541_08795"
     CDS             complement(841..1686)
                     /gene="panB"
                     /locus_tag="P5541_08795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRV72121.1"
                     /translation="MKKNVGIPELMRMKKSGEKIVMVTAYNNWQMRMAEEAGADTVLV
                     GDSLGMVEHGLPDTIGVTMEMMDLHCRAVMRARQRAFVVGDLPFMSYEIDDREAVRNA
                     GNLVKSTGIDAVKLEGGAKRAATVKAIVTAGVPVVGHIGLTPQTTTQLGGYRVQGRDP
                     SGASFLLRDALAIEEAGACALVLECVAAEVAAFLSRRLTIPTIGIGAGPDCDGQVLVF
                     HDIVTLYGEFKPRFVKRFCEGGEALMKGISSYTEEVRKGTFPSPDHSFAANEGTMEHL
                     EAIKE"
     assembly_gap    1761..1860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1861..2701)
                     /gene="panC"
                     /locus_tag="P5541_08800"
     CDS             complement(<1861..2701)
                     /gene="panC"
                     /locus_tag="P5541_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRV72122.1"
                     /translation="MDMPFIAGSPAEARKFLRENARSDRGVGLVPTMGFLHEGHLSLV
                     RRAREENAICVVSIFVNPLQFGPSEDLGAYPRDMKRDLGLLSGEGVDMVYAPSPEVMY
                     LPDRSVLVTENSLSRCLCGAARPGHFDGVCTVVMKLFSAISPDRSYFGQKDYQQLQVI
                     RRMARDLDLAVEIVGCPIIREPDGLAMSSRNIYLGEEERREALALSRSLDVAESMVNN
                     GETSSELILRKVRSVIASSSMVILEYAEIRDAEDLSAVETIDGRAVCALAARVGRARL
                     IDNR"
     gene            complement(2720..4087)
                     /locus_tag="P5541_08805"
     CDS             complement(2720..4087)
                     /locus_tag="P5541_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRV72123.1"
                     /translation="MDERLIRIDYGTAFPGLDPEREIGSVKELLLSSSGKIREGAREG
                     KEGFGWFNLPEADTGPVWRHAEWLRSFDTVIQTGIGGSALGNQMLSRALTHPYYNELT
                     AEERGGPRFYVADNADPESVSSILEMTDPSRTAFIVASKSGATAETMANFLFSLDFLG
                     RNGVEDPASNIIVITDPQKGALRTFAEETGCRVMSVPGNVGGRYSVLSPVGLLSAASQ
                     GADIQAILRGASAMKKQISGTEGKTGNPAWVIAAASMRNYLAGRNMVVLMPYSDRLEA
                     FSEWFCQLWAESLGKDGRGSTPVRALGAIDQHSQVQLYAEGPDDKLYIFITVEERAEV
                     IIPEPQLKSLESLSYLGGKGMGEMLGLEAMSTAAALIKANRPVMGISVPSVDAFRLGG
                     LIFLFEYATAIAGIAQGIDPFDQPGVEQGKRYTYGLMQRPGFGKYAEEAREISSFSRR
                     NSIII"
     gene            complement(4225..4464)
                     /locus_tag="P5541_08810"
     CDS             complement(4225..4464)
                     /locus_tag="P5541_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRV72124.1"
                     /translation="MAKVKVYSTPTCPWCVKMKDYLKSQKVDFENVDVSADREAAMDM
                     VKKTGQMGVPVALIGDVYIVGFDKARIDEELARLD"
BASE COUNT          995 a         1413 c         1075 g         1091 t
ORIGIN      
        1 cggccgcaag gatctgcccg tttatgttgt caatttccgt tcgtcggccc gcttcaatgt
       61 cctgaagcat ggaagccctg ttggaagatg tttttttcag cgtttccatt attgttttcc
      121 ggatttcctc aaggtctatc aggactcctt taagggccat tgcttccact gcctcgccga
      181 gaagcagatc catcaccgcc gcaagatccg ggttgccgat gatggctcca ttgttttgcc
      241 ttacaatcgc tgccaggggg ttgatagcgg cattaaggac cagcttgcgg catatcgcct
      301 cccgcggatc ttccaccagt tggggattaa gatcagccga caggaagagg tctctgatct
      361 tacccaggtc catgcccttc tcccaggggc caaagatcac ggtcccctct ccggcgacaa
      421 ccacttctcc cggagccttc cggtaaccac caaaggtcgt gacaccggcc gcaacccggc
      481 tcgctcccag catcctggca agttcctcgg cgttgcccag accattctga agtgtcagcg
      541 ccactcccat cggggcgagt tctttttcga tcatcggggc aacccttcca gtatcaccgg
      601 atttgacaag cactatcacc aggtcgaaac tacccctccc cgtcccgccc ggtgatacgg
      661 gaatgggcaa tgttttcgta ccgccatcag gaaggtgtat tttcagaccc tttttgctaa
      721 tggcatcgaa atgctctcca ggtctctgta tcaattccag ttcgagaccg gggtatcctg
      781 aaccgagcag gtgaaaagct ataacgcttc ccagggctcc gcaacccgct atagcaactc
      841 tcattccttg atcgcctcga gatgttccat ggttccctcg ttagcagcaa aagagtgatc
      901 gggcgatggg aaggtccctt tcctgacctc ctcggtgtag gaggatatcc ccttcataag
      961 agcctctcct ccttcgcaga accgtttgac aaaacggggc ttgaactctc cgtaaagggt
     1021 cactatatcg tggaaaacca gaacctggcc atcgcagtca gggcctgccc ctatcccgat
     1081 cgtaggtata gtcaggcgtc tgctgaggaa agcggccacc tccgccgcaa cacactcaag
     1141 aacaagcgcg catgcaccgg cttcttcaat ggcaagggca tcccgaagaa ggaaagaagc
     1201 tcccgagggg tccctcccct ggaccctgta accaccaagt tgcgtcgtcg tctggggggt
     1261 caggccgata tgaccgacta ccggaacccc ggcggtgaca atggccttca ccgttgctgc
     1321 cctctttgca ccaccctcca gtttcacagc atcgatacct gtcgatttga ccaggttccc
     1381 ggcgtttctt acggcctccc tgtcgtcaat ctcgtaactc atgaacggga ggtcccctac
     1441 cacaaaggct ctctgtctgg cccgcatgac agccctgcaa tgaagatcca tcatttccat
     1501 ggtcacccct atggtgtctg gaaggccatg ctccaccatt ccaagggaat cccccaccag
     1561 aacagtatcg gcacccgcct cttccgccat ccgcatctgc cagttattgt aggcagtcac
     1621 cattactatc ttttcgccgc ttttcttcat tctcatgagt tcgggaattc cgacattttt
     1681 cttcatgact ctctcctttc gagaaccctg ttgtctatga gtctggcccg tcccaccctc
     1741 gcggcgaggg cgcaaacggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 ccctgttgtc tatgagtctg gcccgtccca ccctcgcggc gagggcgcaa acggcacgtc
     1921 catcgatcgt ctccacagcc gaaagatctt ccgcgtccct tatctcggca tattccagta
     1981 ttaccatgga agaggacgcg ataacgcttc gcactttcct caggatcagt tccgaagatg
     2041 tctcgccgtt attgaccatg gattctgcca catcaagtga acgcgaaagg gcaagggctt
     2101 cacggcgttc ctcttccccg aggtagatgt tcctgctgct catcgccaac ccgtccggtt
     2161 cccttatgat cggacatccg actatttcga cggcaagatc aagatccctt gccatccttc
     2221 tgatgacctg caactgctga taatccttct gtccaaaata cgacctgtcc ggggagatgg
     2281 cactgaaaag tttcattaca actgtgcaga ctccgtcgaa atgccctggc cgggccgctc
     2341 cgcacagaca cctgctcagg gaattttcgg taacaagaac cgacctgtcg ggtaaataca
     2401 tcacctcagg ggacggggca taaaccatat cgactccttc gcccgagagc agccccaggt
     2461 ctctcttcat gtccctgggg tacgctccaa ggtcttcgga gggtccaaac tgcagagggt
     2521 tgacgaagat cgagacaaca cagatggcgt tctcctctct tgcgcgcctg acaagagaaa
     2581 gatggccctc atgtagaaag cccatcgtcg ggacgagccc tactcccctg tcgctacggg
     2641 cgttttccct gagaaatttt ctcgcttccg ccggagaacc ggcgataaat ggcatatcca
     2701 tgatcttctc ccctccacgc tatatgatta tcgaattccg gcgggaaaag gaggatattt
     2761 ccctggcctc ttcggcatac ttcccgaagc cgggccgctg catgagaccg taggtgtacc
     2821 tcttgccctg ttcgacgccg ggctgatcaa aaggatcgat cccctgtgct attccggcaa
     2881 tggcggtagc gtattcgaaa agaaagatca gccctccaag ccggaaagca tcgacggaag
     2941 gaacagaaat gcccatcacg ggccggttcg ccttgatcag ggccgcggca gtggacatgg
     3001 cttccagccc aagcatctct cccatcccct tcccacccag gtaggaaaga ctctccaggc
     3061 tcttgagctg gggttcggga atgattactt ccgccctctc ctccacggtg ataaaaatat
     3121 aaagtttgtc gtcaggacct tcggcataca actggacctg ggaatgctga tcgatcgcac
     3181 cgagagccct cacaggcgta ctccctctcc cgtccttgcc gaggctttcg gcccatagct
     3241 gacagaacca ttccgagaaa gcctccagcc tgtcggagta tggcataagg acgaccatgt
     3301 tccttcctgc gaggtagttt ctcatcgagg cggctgcaat tacccatgcg gggttgcctg
     3361 tctttccttc cgtcccggag atctgtttct tcatggccga agcaccccga aggatagcct
     3421 ggatatcagc cccctgagac gccgccgaga ggagacccac cggcgagaga accgagtatc
     3481 tccccccaac attccccgga accgacatca ccctgcaccc cgtctcttca gcaaaggttc
     3541 tcagggctcc cttttgaggg tcagtaatga caataatgtt cgatgccggg tcttcgacac
     3601 cgttccgtcc aagaaaatcg agggagaaca ggaagttggc catagtttcc gccgttgctc
     3661 ccgacttgct cgccacgata aatgcggtcc ttgaaggatc cgtcatttca aggatagaag
     3721 agacactctc cgggtcggcg ttatcggcaa cataaaacct gggtcctccc cgttcctccg
     3781 cggtcaattc gttgtaataa gggtgggtca gggcacggga aagcatctgg ttccccagag
     3841 cagatcctcc tattcctgtc tggatcaccg tatcaaaaga acggagccat tcagcatgcc
     3901 tccatacagg tcctgtgtcg gcctcaggca ggttgaacca gccaaaaccc tcttttccct
     3961 ctctggctcc ctcgcgaatc ttgcccgagg atgacaataa caattccttc acggatccga
     4021 tttcgcgttc agggtccaga ccggggaagg cggttccgta atcgatccgg atcagtcttt
     4081 catccacagg aacaccaccc ttcagaaaaa ccttaaattt attgacaagt ttacctgatt
     4141 gtaaccactg acaaggacaa aaaaaaggcg ggcccgcctt tttcagcgga tccgccctga
     4201 atattctccg attcttgccc ggcttcagtc aagcctggca agttcctcgt ctattctggc
     4261 cttgtcgaaa ccgactatgt agacatctcc tataagagcc actggtactc ccatctgacc
     4321 cgtcttcttg accatatcca tcgcggcttc cctgtctgca ctaacgtcca cgttctcaaa
     4381 atcgactttt tgtgatttga gataatcttt cattttcaca caccatggac acgtcggcgt
     4441 cgagtaaacc ttgactttcg ccacaacctt tcacctccat gatttttctt tacccgtgct
     4501 tttgcctgaa acatataccc ttcggtggta attctactct ttcattcgtg actgtcaagg
     4561 ttctgcttcc ataacgcaaa cagacccgtt gaccagcccg tattaccgga cctgatcgcc
     4621 cgttttcctt ttggattata ctgtataatc gttgctgaat gaaaaacggg gatc
//
LOCUS       DAOTIQ010000094         4654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_21933_length_4654_cov_5.590346, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000094 DAOTIQ010000000
VERSION     DAOTIQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21933_length_4654_cov_5.590346"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..224)
                     /locus_tag="P5541_08815"
     CDS             complement(18..224)
                     /locus_tag="P5541_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV72125.1"
                     /translation="MSTLSARSEDYLKTIYKLQAERKVVRVKDLAQTLGVRSPTVVGV
                     ISGLKDSGYVDQEHYGYISLTKEI"
     gene            301..1194
                     /locus_tag="P5541_08820"
     CDS             301..1194
                     /locus_tag="P5541_08820"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HRV72126.1"
                     /translation="MHRHTVLGTIAISACLTEVFIAPKPGLVDRIGPGAHKDMDFSTF
                     LLSATALAPYWQEQALPGLEGIPPVNAMKTLRGTGILMDKAMFAATSGINTHKGLVFA
                     LSLLLYGAGRSLFLENRTDISVIVREASSAVRGCCERELSLLSISPPERALTSGERIF
                     LEHGLTGIRGEAERGFPSVVMHGLPSLKKALSNGSSLHDSALYSLFNLMLHCEDTNIV
                     ARKGFLFWKDEYPGLVTPLFQLFPPYGQEELEHLKRADRYFSTHGISPGGAADLLTCT
                     LFLHHCETVALQHCKHDNARV"
     gene            1225..1602
                     /locus_tag="P5541_08825"
     CDS             1225..1602
                     /locus_tag="P5541_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV72127.1"
                     /translation="MSLGLRMKTLRKSKGLTQKTLADMVEVSRIYIQSLESNRRLPSM
                     KLLRRLAGALDVDVVDLVQDPHRDVSGKLLLEEVLDNREPVEIWYKSKRLSRQEVQLV
                     HKLIDAALSDWDREDVGQDRPEG"
     gene            1574..>1857
                     /locus_tag="P5541_08830"
     CDS             1574..>1857
                     /locus_tag="P5541_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72128.1"
                     /translation="MSGRIDQKDSPPLFEWPAHPDVIPGWALDEALPWRGLDDFDLLV
                     LAEEKCGRIVELKHVPLPEKLWGLHVARGKRARIYVNRRLPPIWRRFSLF"
     assembly_gap    1858..1957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1958..2154
                     /locus_tag="P5541_08835"
     CDS             <1958..2154
                     /locus_tag="P5541_08835"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRV72129.1"
                     /translation="IYVNRRLPPIWRRFSLFHELYHLLYHKRGESFWSGTATPMSSFE
                     HQADMFAWAAIREDWAEGAL"
     gene            2151..3110
                     /locus_tag="P5541_08840"
     CDS             2151..3110
                     /locus_tag="P5541_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV72130.1"
                     /translation="MNRLPFFLPMRNCPNRCIYCDQMTITGHHDMPSPDDVRSMASRA
                     SGPVETCFFGGSFTCLPENTRREYLSAASSAPPGSSIRISTHPDCINPGALSILGDYP
                     VTIVELGISSLDDRVLEACNRGYTGKTALEKISELLDDGRFIPGVQMMTGLPGQTPES
                     SMEDLRKIAALKGNRRMQLRIYPCLVLANTPLEKLYSSGKYKPYSLEESARWAGEMIL
                     FARDNDFELLRVGLQETESLGQGVVAGPHHPALGEYARSCALTMDIFAKAPRGPWALS
                     SRDMSLIYGHGGWGLTELARLSGLEASEAENRITWSDRHPPCI"
     gene            3138..4529
                     /locus_tag="P5541_08845"
     CDS             3138..4529
                     /locus_tag="P5541_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV72131.1"
                     /translation="MALLHRNVDMGRGSVPVTIWRLALPSILSMLFHTLFHLVDTVFV
                     SWLGESSLAAMSLTFPLVFVVFALVNGMAVGSTTRMSQSLGGGRENEARHYADAALIL
                     VLILSAPVFPLLLRPFSNLFFSFLGGTGQVLPESYSYAFWMVLATPLMSYSLLADSVF
                     RSQGDTVTPMKSMILGNGINILLDPLFIFTFGWGIAGASVATLIGRLASCVYLWSRLR
                     KRSSIRPAFSWDRGHPARWVDIGRVGLPVAISQGSMSIGAAMLNRILSGFGPAAIGAW
                     MLGNRVEMLVFLPVFGFNGAVIPFVGYNLGRGDYGRIRESLRVVLFSSFAMISIAGTL
                     VYLFPETILALFKPSGQVMAMATASIRASATAYLLAAADISFWGIFQGSGYPVYGMIA
                     QIVRTLAVRVPAALLLSAVFGLAGVWWCQPISAAASFLISTYFIFVVMKRIKNDIENR
                     KAVDRRIKETGDR"
BASE COUNT         1048 a         1228 c         1216 g         1062 t
ORIGIN      
        1 accgacggtt gtcggggtca tatctctttc gtgagagata tgtacccgta atgttcctga
       61 tccacatacc cggaatcctt gagccccgat atgaccccga caaccgtcgg tgaccggacg
      121 ccaagggtct gggcaagatc ctttaccctg acgaccttgc gctccgcctg gagtttgtat
      181 atcgtcttga gataatcttc acttctggcg ctgagagtcg acattgttag ccccgcctat
      241 ttttgttatc atccgtcaca gtatccactt ttaccccttt cgggtcaagg gagtgaatca
      301 atgcatcggc atactgtcct gggaacgata gccatttcag cctgtctgac agaagtattc
      361 atagccccca aaccgggcct ggtcgaccga attgggcctg gagctcacaa ggacatggat
      421 ttctcgactt tcctcctgag cgcgacagcc ctggccccct actggcagga gcaggcactc
      481 ccggggctgg agggaatacc gcctgtcaac gccatgaaga cacttcgcgg gaccggtatc
      541 ctgatggata aggcaatgtt tgcggcaacc tctggaataa atacccacaa gggacttgtg
      601 ttcgctcttt cactgctgct ttacggggcc ggaagatctc tttttctgga aaacaggaca
      661 gacatttccg tgatcgtacg cgaagcatct tcagcagtcc gcgggtgctg cgagagagaa
      721 ttgagtcttc tgtccatctc ccccccggaa agagccctca ccagcggaga gaggatcttt
      781 ctggaacacg ggctgacagg cataagagga gaagccgaaa gggggttccc ctcggtggtc
      841 atgcacggcc tgccgtcact caaaaaggct ctttcaaatg gctccagtct tcatgacagc
      901 gctctttact ccctcttcaa ccttatgctt cactgcgagg atacgaacat agtagcaaga
      961 aaggggttcc ttttctggaa ggatgaatac cctggccttg tcactccgct gtttcaactc
     1021 tttccgccat acggacagga agaacttgaa cacctgaaga gggccgacag atatttttca
     1081 acccatggga tcagccccgg aggcgcggca gacctgctta cctgcacgct ctttctccat
     1141 cattgtgaaa ccgtggcctt acagcattgt aaacatgata acgccagagt ataatatatg
     1201 taatacaacc ctggaggtga agtcatgagt ctcggattga gaatgaagac tctccggaaa
     1261 tccaagggcc ttacccagaa aacgcttgcg gatatggtcg aagtaagccg gatatacatt
     1321 cagtccctgg aaagcaacag gcgtctccct tccatgaaac tgctccggag actggcgggg
     1381 gctcttgatg ttgatgtggt agacctggtc caggacccgc acagggatgt atcgggaaaa
     1441 cttcttctcg aagaagttct ggacaaccgc gaacctgtgg agatatggta caagagcaaa
     1501 agactttcca ggcaggaggt tcagcttgtt cataaactta tcgatgctgc cctctcggac
     1561 tgggacaggg aagatgtcgg gcaggataga ccagaaggat agccctcccc ttttcgaatg
     1621 gcctgcccac ccggacgtca tacctgggtg ggctcttgac gaggcattgc cgtggagagg
     1681 ccttgatgat ttcgaccttc tggtcctggc tgaggaaaag tgcggcagga tagttgaact
     1741 gaagcatgtt cctcttcccg agaaactgtg gggacttcac gtggcgaggg gcaaaagggc
     1801 acgaatatac gtaaacaggc gcttgccgcc gatatggagg cgtttttcat tgttccannn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa tatacgtaaa caggcgcttg
     1981 ccgccgatat ggaggcgttt ttcattgttc cacgaacttt atcatctgct ttaccacaaa
     2041 agaggagagt ctttctggtc cggtacggcc acccccatgt ccagtttcga gcatcaggcc
     2101 gacatgtttg cctgggcagc gatccgggaa gactgggccg agggtgccct gtgaaccggc
     2161 ttcctttttt tctccccatg agaaactgcc ccaacaggtg catctattgt gaccagatga
     2221 ccattaccgg tcaccacgat atgccgtcac ccgatgatgt caggtctatg gcttccaggg
     2281 ccagcggccc cgtggaaacc tgttttttcg gaggcagttt tacatgcctg cccgaaaaca
     2341 cgagaaggga gtacctttct gcagcatcgt cggctccgcc cggaagcagc atcagaatat
     2401 cgactcaccc ggactgcata aaccccgggg ccctgtcgat cctcggagac tacccggtca
     2461 ccatagtgga actcggcata tcatccctcg acgacagagt tctggaggcg tgcaacaggg
     2521 gctacacggg caaaacagcc ctggaaaaaa tatcggaact tcttgatgac ggccggttta
     2581 ttccgggggt tcagatgatg actggcctgc ctggccaaac tcccgagtca tccatggagg
     2641 accttcgaaa aatagccgct ctgaagggca atcgcagaat gcagctaagg atatatccat
     2701 gtcttgtcct ggccaatact cccctggaaa agctctacag ttccgggaaa tacaaacctt
     2761 acagcctgga ggaaagcgca agatgggccg gagagatgat ccttttcgcc cgggataacg
     2821 actttgaact gctccgggtc ggcctgcagg aaacggaatc tctgggccag ggagtcgtgg
     2881 ccggacccca ccatccagcc cttggagaat acgcgaggtc ctgcgccctt acgatggata
     2941 ttttcgcgaa agccccacgc ggaccctggg cgttgtcatc cagggatatg tctctgatct
     3001 atggccacgg aggctggggg ctcacggagc tcgcaaggct ctctggcctg gaggccagcg
     3061 aagcggaaaa caggataaca tggtccgaca ggcatccccc ctgtatctga tagcctgata
     3121 aaggcaggag gtaagttatg gcactccttc atcgcaatgt cgatatgggc aggggaagtg
     3181 tacctgtcac catctggagg cttgctctac cctccatact ttccatgctt ttccatacgt
     3241 tgttccacct tgtggacact gtattcgtct catggctcgg ggaatcttcc ctggcagcca
     3301 tgtcactgac ctttcccctg gtttttgttg ttttcgccct ggtcaacggt atggctgtag
     3361 gatcgacgac gaggatgagt cagtccctgg ggggaggtcg tgagaacgag gccagacatt
     3421 atgccgatgc cgcgcttata cttgtcctta tactttccgc cccggtattt cctcttcttc
     3481 tcagaccctt ctcaaatctg tttttctcct tcctcggagg aacgggacag gtccttcctg
     3541 aaagttactc ttacgccttc tggatggtcc tggccactcc gctgatgtca tattcactcc
     3601 tggccgactc ggtattccga agccagggag acacggtcac tcccatgaaa agcatgatcc
     3661 tgggcaacgg gataaacatc ctgcttgacc ccctcttcat ttttaccttc ggctggggga
     3721 tagcaggagc atcagttgcc accctcatag ggcgtcttgc ctcctgcgtc tatctctggt
     3781 caaggctacg aaaaagaagc agcatcaggc ccgcattctc atgggacagg ggacaccctg
     3841 ccagatgggt ggatataggc cgggtcggat tgccggtggc aatttcccag ggcagcatgt
     3901 caattggagc ggcaatgctg aacaggatcc tttccggttt cggccctgcg gccatcggag
     3961 cgtggatgct cgggaaccgg gtcgagatgc tggtatttct gcctgttttc gggttcaacg
     4021 gagccgtgat acctttcgtg gggtacaacc tcggcagggg tgactacgga aggataaggg
     4081 aatccctccg ggtggttctt ttctcgtctt tcgcaatgat aagcattgca ggaacattgg
     4141 tttacctctt ccccgaaacg atccttgccc ttttcaagcc atccggacag gtaatggcaa
     4201 tggcaactgc ctccataagg gcatcggcta ccgcatacct tctggcagca gcagacatct
     4261 cattctgggg gatcttccag ggaagcggtt accccgttta cggaatgata gcccagatag
     4321 tcaggacact tgcggtcagg gttcctgccg cacttctgct gtccgcggtc tttggcctgg
     4381 cgggagtctg gtggtgccag ccaatatctg ccgccgcgtc attcctgata tcaacatatt
     4441 ttattttcgt tgtaatgaag aggatcaaaa acgatataga aaacagaaaa gccgtggatc
     4501 gaagaatcaa ggagactgga gacaggtaaa aggagtcagg aaaacctaaa aaaacaattc
     4561 agattctgac cctaaaaccc tctcaccaca ggggacacag atggtgaagc cacacagaga
     4621 aaacctttct ttgtgaggac cggggacgca agaa
//
LOCUS       DAOTIQ010000095         4623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_22129_length_4623_cov_4.424912, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000095 DAOTIQ010000000
VERSION     DAOTIQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22129_length_4623_cov_4.424912"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="P5541_08850"
     CDS             complement(<1..618)
                     /locus_tag="P5541_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72132.1"
                     /translation="MNLPFEAFEKASIGELAEGVMEDPETKAKVCLKCGKTFAAGEVF
                     QLEDRYWEANHAARIHSEREHEPQFNYLLNLMKQWRGVSDPQAKLLEAFFRGDDDGRI
                     ASDLGISRSTVRNHRFRMKERARHAKVFMAMMQILDQKEKPEARLVAFPANTRIADER
                     FDLTSEECERIIGKYFREDGTMTRFPIKAKERVALLRRIVGLLEPG"
     gene            870..1238
                     /locus_tag="P5541_08855"
     CDS             870..1238
                     /locus_tag="P5541_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRV72133.1"
                     /translation="MKKEIRTAKSPDPIGPYSQGLVVGNRIYVSGQGPLNPETGKTPS
                     TIEEQTRQVMTNIKNILEAGGATMDDVVKSTVHLADLNDFDAFNGVYKEFFKAPFPVR
                     TTVGSSLLDILVEIDAIAEI"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1403..1773)
                     /gene="alaE"
                     /locus_tag="P5541_08860"
     CDS             complement(<1403..1773)
                     /gene="alaE"
                     /locus_tag="P5541_08860"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HRV72134.1"
                     /translation="MGKKGTGVNGASFVNSVKRGRLVMIAADIIAMTVFSFVLCMFIE
                     VFIAGLSVFQSLQARAAAIPVNLITGRPYGCFRDWLFRRLGIERKTPFKAVIGDTLAF
                     VVFQVPLYVVVLLFAGATWRQ"
     gene            complement(1806..3011)
                     /locus_tag="P5541_08865"
     CDS             complement(1806..3011)
                     /locus_tag="P5541_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV72135.1"
                     /translation="MLYRKMPKNGDELSILGFGCMRLPVKDGSIDEERAMAQVRFAID
                     QGVNYIDTAWTYHAGESENFLGRALRDGYRERVKLATKLPSYLVKTREDMDMFLDTQL
                     GKLQTDHLDYYLLHNLTGPLWDSIDVLGVRSFLDSAKTDGRIVNAGFSFHGLPSDFNR
                     IVDAYDWDFCLIQYNYLDEENQAGTRGLEYAASKGMGVFVMEPLRGGNLGIPEAPPAI
                     QEIWNEASLSRTPAEWALRWVWNRPEVKVVLSGMNEEAHIKENLGIAEEARPGSLSAG
                     ELALVKRAAGKYREIMKVNCTGCGYCTPCPAGVNIPAAFEVYNKMHLFGNFQEGKMNY
                     VIKLSGIVRKGVTSYASQCVECGQCLEKCPQSLDIPTLLKDVVAELEGPDMEELAARA
                     RKSLNLESA"
     gene            complement(3110..4045)
                     /locus_tag="P5541_08870"
     CDS             complement(3110..4045)
                     /locus_tag="P5541_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRV72136.1"
                     /translation="MRKVPALREGDTIAFVSPSSQSNRESVEKALKITEDMGFHVKVS
                     ENCDGFGEYPGTLDEARAEEINRAFLDTDIRGIFCIGGGYGAIRLPGLIDMGSAFRNP
                     KVFIGYSDSTILHILFNRPGGFITFHGPMPVSDFSRGTFPGYVRESLLKAVCSTKPLG
                     EIVPFEGAPPVETLVPGRAEGKLAGGNLSMVCSLLGTPWEIDTRGKILILEETGEPVY
                     MIDRMLTQLRLAGKLEAAAGIVLGQFTHILPGKPKEPFTITDVFETVVAPVGKPTLIN
                     GCFGHDVKKATIPLGARAVIDGATSTLTVVEAGVE"
     gene            complement(4070..>4623)
                     /gene="truD"
                     /locus_tag="P5541_08875"
     CDS             complement(4070..>4623)
                     /gene="truD"
                     /locus_tag="P5541_08875"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRV72137.1"
                     /translation="CPEPALKPIVEALKAGEGAIGALRKVPREEMGQYLSSYQSFLWN
                     MVLKGSLEQRTITLFEIPCITGTLLQYEDLSEDELAFLSLLQVPTVGADMLPLTEGIK
                     RIMSRVLFERGVKLAKFRLPEIPQAYFASFLRPAIVIPGELKWEAAPDSLFPGRQMLR
                     LEFFLPRGSFATMLIKSIGGETE"
BASE COUNT          979 a         1264 c         1205 g         1075 t
ORIGIN      
        1 ccccggctcc agaagcccca cgatcctgcg caaaagggcc acccgttcct ttgccttgat
       61 cgggaagcgg gtcatggtgc cgtcctccct gaagtatttc ccgatgatgc gttcgcactc
      121 ctctgaggtg aggtcaaaac gctcgtccgc gatgcgcgtg ttggccggaa aagcaaccag
      181 cctggcctcg ggcttttcct tctgatcgag gatctgcatc attgccatga agacttttgc
      241 gtgcctggct cgctccttca tacggaaacg gtgattcctt acggtggagc ggcttatgcc
      301 cagatccgag gctatccttc catcgtcatc cccccggaag aatgcctcca ggagctttgc
      361 ctggggatcg gagacgcccc tccactgctt catgaggttc agaaggtagt tgaactgcgg
      421 ttcgtgctct ctttcggaat ggatccttgc cgcatggttg gcctcccagt aacggtcttc
      481 caactggaag acctcccctg ccgcaaaggt ctttccgcac ttcaggcata ccttcgcctt
      541 tgtttcagga tcctccatta caccttcggc cagctcccct atggatgcct tctcgaaggc
      601 ctcaaagggt aaattcataa acaatcacct tccttgtttg ctcatgaaca aacatatagt
      661 cctttttgtt tgtaatgtca agcttcacct cgttctccta ggccttttcg atttctttgg
      721 ccttacagcg atattcccgg caatgatgaa gatcgcttat tactctatac ttatccggag
      781 ggagaggtga acatggcctg gcgatccata aggttcttcg attcacgaat aacgatttac
      841 gaatcacgta aataagggag gttttcaaaa tgaaaaagga gatacgcacc gcgaagtcgc
      901 cagatccgat aggtccctat tcccaggggc tggtagtggg taaccgtatc tacgtatcag
      961 gacaggggcc gctgaaccct gaaacgggaa aaaccccgtc gacgatcgag gagcagacga
     1021 gacaggtcat gaccaacatc aaaaacattc tggaagcagg gggggcaacc atggacgatg
     1081 ttgtaaaatc cacggtccat cttgccgacc tgaatgattt tgatgctttc aacggggttt
     1141 acaaggagtt tttcaaagcc ccgtttccgg tgagaacaac tgtcggaagc agccttctcg
     1201 atatactggt ggagatcgac gccatcgcgg agatctagaa aaaactgtga ggagatgatg
     1261 gccgtggcgc gctggttcct gatagttggc gggatccttg ttnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnatctgtct ccatgtagcc cccgcgaaga gaagaacaac
     1441 tacataaagc ggcacctgga atacaacaaa agccagggtg tcaccaatga ccgccttaaa
     1501 gggggtcttt ctttctatcc cgaggcgtcg gaagagccag tccctgaagc acccgtaagg
     1561 cctcccggtg atcaggttga cggggatagc cgcggccctc gcctggagtg actgaaatac
     1621 cgaaagcccg gcaatgaaga cctcgatgaa catgcacagg acgaaggaga acaccgtcat
     1681 ggcgatgata tcggcggcga tcattacaag cctgccccgt ttgacgctgt tgacaaagga
     1741 tgctccattc actcctgtcc ccttctttcc caaggcagta tcccccgctg gatctccgat
     1801 cgggttcaag ccgattcaag gttaagggat ttccttgccc ttgcagccag ttcttccatg
     1861 tccggacctt ccagttcggc tacaacatcc ttgagaaggg tcgggatgtc caggctctgg
     1921 ggacacttct cgagacactg gccgcactcg acgcactggg aggcgtaact tgtgacgccc
     1981 ttgcggacga tcccgctcaa tttgatgacg taattcattt tcccttcctg gaagttcccg
     2041 aaaagatgca tcttgttgta gacctcgaag gcggcaggga tgttgacccc ggcaggacag
     2101 ggggtgcagt aaccgcatcc ggtacagttg accttcatta tctccctgta ttttccggcg
     2161 gcccgcttta ccagggccag ttccccggcg ctcagagagc cgggtcgggc ctcctcggcg
     2221 ataccgaggt tttctttgat atgggcctct tcgttcattc ccgaaagtac gaccttgacc
     2281 tccgggcggt tccagaccca tcgtaacgcc cactcggcgg gtgttctact taaggatgcc
     2341 tcgttccaga tttcctggat agccgggggg gcttccggaa taccaaggtt gccgccccgc
     2401 aggggttcca tcacgaagac acccatacct ttcgaggcgg cgtactccag accccttgtt
     2461 ccagcctggt tctcctcgtc gaggtagttg tactgtataa ggcagaaatc ccagtcgtac
     2521 gcgtcgacga tgcggttgaa atctgacggt aacccgtgaa aggagaaccc ggcgttgacg
     2581 atccgtccgt ctgtttttgc cgagtcgagg aaacttctga cacccagaac atcgatgctg
     2641 tcccatagag ggcctgtaag gttgtggagc aggtaatagt ccaggtggtc cgtttgcagt
     2701 tttcccaact gtgtgtccag gaacatgtcc atatcctcac gggtcttgac caggtaggag
     2761 gggagtttgg tcgcaagttt aacccgttcg cggtagccgt cccgcagggc tcttcccaga
     2821 aaattttcgc tctcccctgc atggtaggtc catgccgtgt ctatgtagtt cactccctgg
     2881 tcaatggcga agcgcacctg ggccattgcg cgctcctcgt cgatggagcc gtctttcacg
     2941 ggaaggcgca tgcacccgaa tccaagtatc gaaagttcgt cgccgttttt cggcattttg
     3001 cgataaagca tcacgatatc tcctctctct aatgaaaaat aaggctgtgt tcaaagtctg
     3061 atattggatc ctcagatttt cgggtttttg cgattcacga atcccggtct tactccaccc
     3121 ccgcttccac aacagtaagg gttgatgtag cgccgtcgat cacggccctg gcacccaggg
     3181 ggatggtcgc cttcttgacg tcatggccga aacagccgtt tatcaaggtt ggtttgccga
     3241 cgggtgccac tactgtttcg aagacatcgg tgatggtgaa gggttccttc ggttttcccg
     3301 gcaggatatg ggtgaactgt cccaggacta tcccggctgc ggcctcaagt tttcccgcca
     3361 ggcgaagctg ggtaagcatc ctgtctatca tgtatacggg ctccccggtt tcctccagaa
     3421 tgagtatctt gcccctggta tctatctccc agggcgttcc cagaagagag cagaccattg
     3481 aaagatttcc gccagccagt ttgccttcgg ctctccccgg gacgagggtc tccacaggcg
     3541 gggctccctc aaaaggcact atttccccca gaggtttcgt ggagcagacg gccttgagaa
     3601 ggctctcccg aacgtagccg gggaaagtcc cccgggaaaa atcgctgaca ggcataggtc
     3661 cgtgaaaggt gatgaagccc cccggcctgt taaacaaaat gtggagtatg gtggaatcgc
     3721 tgtacccaat gaagaccttc ggattccgaa aggccgaacc catgtcgatc agtccgggaa
     3781 gcctgatggc tccgtaccct cccccgatgc agaagatccc gcggatatcc gtgtcaagga
     3841 aggccctgtt gatttcttcc gcacgagcct catcgagggt tccgggatac tccccgaatc
     3901 catcgcagtt ttcggaaacc ttgacatgga aacccatgtc ctcggtgatc tttaacgctt
     3961 tttcgaccga ctcccggttc gactggctcg aaggggagac gaaggcaatg gtatctcctt
     4021 ctcgaagtgc cggaactttt cgcatgaaga tcactctccc gcgatgtaat tactccgttt
     4081 ccccgcctat ggattttatc agcattgtgg cgaaggagcc ccggggtaga aaaaactcga
     4141 gtctgagcat ctgtcgaccg gggaacagac tgtcaggagc cgcttcccat ttcagttcgc
     4201 cgggtataac tatggccgga cgtaaaaatg atgcgaaata tgcctggggg atctccggca
     4261 gccggaactt ggcaagcttg acccccctct caaaaagaac gcgcgacatt atccgtttaa
     4321 tgccctctgt cagaggcagc atgtcggctc cgaccgtggg tacctgaagt agggaaagaa
     4381 atgcaagttc gtcttcggaa aggtcttcgt actggagcag tgtccccgta atacacggga
     4441 tctcgaagag agtaatcgtc cgctgttcga gtgatccttt caggaccatg ttccacagga
     4501 aactctggta tgaggataga tattgtccca tctcctcccg ggggaccttg cgcagggctc
     4561 ctatcgctcc ctcaccggcc ttaagggcct cgacgattgg tttgagggcc ggttccgggc
     4621 aga
//
LOCUS       DAOTIQ010000096         4577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_22414_length_4577_cov_6.109465, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000096 DAOTIQ010000000
VERSION     DAOTIQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22414_length_4577_cov_6.109465"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..195)
                     /locus_tag="P5541_08880"
     tRNA            complement(109..195)
                     /locus_tag="P5541_08880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Leu,seq:gag)
     gene            complement(413..1339)
                     /locus_tag="P5541_08885"
     CDS             complement(413..1339)
                     /locus_tag="P5541_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRV72138.1"
                     /translation="MDPFLMVLPLALVMALGVVFGRIGLLTEDTVPKLNAILYWAALP
                     ALLFRSILRVGGGIFSDPNLFWAVHVSFILIPGIAWLISSRFTRERRKIAVSVLVAVR
                     ANNVFMGIPAITIALGQPGLDALSLFFAVGLLGYNFISITWAQAALSGKVSLRSLGAT
                     LINLLKNPLILACLFGVSGAFMGYSELPWWIDSTLKIIGDTGSGIALIALGASLKIHN
                     PLRALKSTWRDIFFKLMIHPALVWVTFLVWPVDPVLRDTVVLVSAMPAAINNFVLAQS
                     MGMDSDHAGEIVALSTAFSIITLPIWIRILGV"
     gene            complement(1444..1791)
                     /gene="rplS"
                     /locus_tag="P5541_08890"
     CDS             complement(1444..1791)
                     /gene="rplS"
                     /locus_tag="P5541_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRV72139.1"
                     /translation="MDARIALVESRYMRKETPEFRPGDTVKVNVKVTEGNRERIQVFE
                     GVVIARKHGGMRETFIVRKISGGVGVERIFPLNCPSVDSIEVVRHGRVRRAKLYYLRK
                     LSGKAARIKERRY"
     gene            complement(1828..2985)
                     /gene="trmD"
                     /locus_tag="P5541_08895"
     CDS             complement(1828..2985)
                     /gene="trmD"
                     /locus_tag="P5541_08895"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRV72140.1"
                     /translation="MKITVITAFPELLEPFRIHGVIGKAVEKGILEITCINPRDHANG
                     HYGQIDDYAYGSGGMVLMAEPLARAVDEARKGSPAMVLFPGPQGIPLEQHLVESLAVN
                     EHIVIVCGRYEGVDERFVDEYVDLEISIGDYVLTGGELPAMVIVDAVARMIPGVVGQE
                     SAVEEDSFFRGMLDTPHYTRPSEWRGRKVPEVLTGGDHEAIRSWRRSRSVARTVERRP
                     DIVAAANIGPYLDKGVYVILVPAGEALPEDIQASASASASFGCGRLLCIVGDGDERKR
                     IQTLAGEGVKVVPSIGGAFSWIRKREKTDPFVIIPGSGVVSEHWIEIKRKALVSGRPV
                     VFVIGLRDYKKIPAGIEVAVLGPITGGKGETSLLEARETAAVILDRFFGWR"
     gene            complement(2985..3515)
                     /gene="rimM"
                     /locus_tag="P5541_08900"
     CDS             complement(2985..3515)
                     /gene="rimM"
                     /locus_tag="P5541_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRV72141.1"
                     /translation="MGEPQMTIIGRVLGAHGVRGEFRVLPLTDFPERFLDMECMDIFR
                     PAGKLLASISVEGIRSHEGKGVFLVSSPQVRDRECAQSLEGGLVMIARSERVSLPEGS
                     FWIDDVIGLEVLHADTGESFGNVLEVMKTGEHDIYVIQSGDGTTRMLPAVREAVLRIA
                     PDEGFMEVRPPEGLWD"
     gene            complement(3525..3770)
                     /locus_tag="P5541_08905"
     CDS             complement(3525..3770)
                     /locus_tag="P5541_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRV72142.1"
                     /translation="MPDYEELVGYVVKNIVSNPDEVTVTSRRSSGGVVSVSIRVAQDD
                     MGRLIGKRGVTINAIRLVARAAAVKPGEKVDVEILED"
     gene            complement(3780..4049)
                     /gene="rpsP"
                     /locus_tag="P5541_08910"
     CDS             complement(3780..4049)
                     /gene="rpsP"
                     /locus_tag="P5541_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRV72143.1"
                     /translation="MAVRIRLARFGRKKQPYYRLVVADSRAPRDGAFIEQIGSYDPMK
                     DPAALKVEEERALYWLGVGALPSDTARAILKKTGVWEKFTAGRKK"
     gene            complement(4113..>4577)
                     /locus_tag="P5541_08915"
     CDS             complement(4113..>4577)
                     /locus_tag="P5541_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRV72144.1"
                     /translation="ILGMGDVQGLAEKVKLAVDDGDMEKIAASVGRKKMTMDDMLLQI
                     RQVRKMGPLDKILEMMPGSANIKGLQGAEMDPRRLARVEAIILSMTREERGNPQIIKG
                     GRRRRIAMGSGTSVQMVNQVLKQFQQMNELMKRFAPGKRGMKLPKGFPPVRM"
BASE COUNT         1042 a         1364 c         1111 g         1060 t
ORIGIN      
        1 attcttgatg aaaacgaaag gaagaaacga ctccacaaag ccgtctccta gaacctgcag
       61 aatataacac caaggccgcc cctgggggcg gccttttttg ttttgtaatg gtgcggaggg
      121 ggagacttga actcccacag cccgtaagcc accaggccct caacctggcg cgtctaccta
      181 ttccgccacc cccgcatttc cacgatgcga gtgacattat aggcaagttg cactggggcg
      241 tcaagggaga aatccgtgac tgaaaaaaca aggcatcagg aggctaggtt taactgttat
      301 tactgagttg accttgttga ccttgttgac cttgttgacc ttgtcgacct tatcgtcgtt
      361 gggggcaatg ctgtcaaatg ggtcaataag gtcacgagtt acgatcccct gtttacactc
      421 caaggatcct gatccagatc gggagggtta ttatagaaaa ggcagtgctt agggcaacta
      481 tctctccggc atgatcggaa tccattccca tgctctgggc caggacaaag ttgtttatgg
      541 ccgctggcat tgccgagaca aggaccaccg tatcacggag gaccgggtcc acgggccaca
      601 ccaggaaggt cacccaaacg agggcggggt ggatcatcag tttgaaaaaa atatctctcc
      661 aggtgctttt cagagccctt aacgggttat gtatcttgag ggaggcacca agagcgatga
      721 gagcgatccc gcttcctgta tcgccaatta ttttgagggt agagtctatc caccacggaa
      781 gttccgaata acccataaag gctccggaca ctccaaaaag acaagccagt atgagggggt
      841 ttttcagaag gtttatcagg gtcgccccga gggatctcag ggacaccttc ccggaaagag
      901 ccgcctgcgc ccaggttatg gagataaagt tgtaaccgag aagccctacg gcaaaaaaca
      961 gggaaagggc atccaggcca ggctgaccaa gtgcaatggt tattgcgggg attcccatga
     1021 agacattgtt ggccctcacg gccaccagaa ccgaaacggc tatcttcctt cgctccctcg
     1081 tgaatcgcga agagatcagc caggcgatcc ccggaatgag aataaaggag acgtggacgg
     1141 cccagaaaag attcgggtcg gaaaatattc ctcccccaac cctgaggatg gacctgaaaa
     1201 gaagtgccgg gagagcggcc cagtaaagga tagcgttgag tttagggaca gtatcttcag
     1261 taaggagtcc gatacgccca aaaacaacac ccagcgccat cacaagcgca agggggagaa
     1321 ccataaggaa tggatccatg ttttctcctc cttccggacc accaaaaggc aaaaaacagg
     1381 aaaaggccgt cgcgccggca ggttccggct gcgacggccg ctatctggac aaaaccttca
     1441 ggtctagtaa cgtcgctcct tgatacgggc ggccttaccg ctgagcttgc gcaggtagta
     1501 aagtttcgcc ctgcggaccc tgccgtgacg aaccacttcg atactatcga cgctggggca
     1561 gttcagaggg aaaatcctct ccactccaac accgcccgag atcttcctga caatgaaggt
     1621 ctcgcgcata cccccgtgtt tcctggcaat aacaacgcct tcgaaaacct ggatcctttc
     1681 cctgttaccc tcggtaacct tgacgttcac cttgacggtg tcgccggggc ggaattcggg
     1741 cgtttccttt ctcatgtatc tgctctctac aagagctatt ctggcatcca tttcaatacc
     1801 cctccctgtt gaatccttgt tgtctcttta acgccagcca aagaaccggt caagtattac
     1861 ggctgccgtt tcccgggctt ccaaaaggga tgtttctccc tttccacccg ttatcggtcc
     1921 caggaccgca acttcgatcc ctgccggtat ttttttgtaa tcacgcaaac caataacaaa
     1981 aacgacaggc cttcctgaaa caagggcttt cctttttatt tcgatccagt gttcgcttac
     2041 gacccctgat ccgggaatta tcacgaaagg atcagtcttt tcccgtttcc gtatccagga
     2101 gaaagcccct cctatggaag gaaccacctt tactccttcg ccagcaaggg tctggatccg
     2161 ctttctttca tcaccatcgc cgacaataca gagaagcctg ccgcaaccaa aggaagcaga
     2221 agccgacgcc gacgcctgga tatcttcggg tagggcttcc ccggcgggga caagtatgac
     2281 ataaacgccc ttgtcaaggt aggggccaat gtttgcggca gcgactatgt caggtcttct
     2341 ttctaccgtc ctggcaacgg acctgcttcg cctccacgat cgtatggcct catggtctcc
     2401 accggtcaaa acttccggaa cctttctccc ccgccactca gacggccggg tgtagtgcgg
     2461 tgtatcgagc attccacgga agaaagagtc ctcctccacg gcgctttcct gtcccacgac
     2521 cccgggaatc atcctcgcta cggcatcgac tatcaccatt gccgggagtt ctccccctgt
     2581 aagcacgtaa tcccctatgg atatctcgag atccacgtac tcatctacga aacgttcgtc
     2641 cacaccttcg tatctgccac atactattac tatgtgttcg ttgacggcaa gggactccac
     2701 caggtgctgc tcgagtggga tcccctgggg acccgggaag agaaccatgg ccggcgatcc
     2761 ctttctggcc tcgtccaccg ctctcgcgag aggttcggcc ataagtacca tccccccgct
     2821 tccgtaggcg tagtcatcga tctgtccgta gtgcccgttt gcatggtcgc gaggatttat
     2881 gcacgtgatc tcgagtatac ccttctcaac agcctttccg ataaccccat ggatccggaa
     2941 tggttcaagg agttccggga aagccgttat tacggttatt ttcatcagtc ccagagcccc
     3001 tcggggggac gaacctccat gaatccttca tccggggcaa tcctcaggac cgcttccctg
     3061 actgccggaa gcatccttgt ggtgccatcg ccggactgga taacgtaaat gtcgtgttcg
     3121 ccggttttca tcacttcaag gacattaccg aaagattctc ccgtatcggc atgcaagacc
     3181 tcaaggccaa taacgtcgtc tatccagaaa ctcccctcag ggagagaaac ccgttcggac
     3241 cgggctatca tcacgagacc tccctcaagg ctttgagcac actccctgtc tcgcacctgg
     3301 ggcgaagaaa ccaggaaaac ccccttaccc tcgtgagacc ttattccttc gacactgatc
     3361 gaagccagaa gttttcccgc cggtcgaaat atatccatac actccatgtc gagaaaacgc
     3421 tccgggaaat ccgtgagcgg gagaaccctg aactcgccgc gtactccgtg cgcgccgaga
     3481 acacgtccta ttatcgtcat ctggggttcg cccatggtat cacctcaatc ttccagaatc
     3541 tccacgtcta ccttttcccc gggttttact gcagcagccc tggcgacaag ccttatcgcg
     3601 ttgatggtta ctcccctttt cccgatcaga cggcccatat catcctgggc tacccttatt
     3661 gaaacgctga ccactccccc gctgctgcgg cgcgaagtca ccgttacctc gtcggggttg
     3721 gagactatgt tcttcaccac gtaaccgacg agttcctcgt aatcgggcat agcaaaggcc
     3781 tactttttcc tgccggctgt aaacttttcc cagacgcccg ttttcttgag tatcgcccgg
     3841 gccgtatccg agggaagcgc tcctacacca agccagtaaa gagccctttc ctcttcaacc
     3901 ttgagcgcag cggggtcttt catgggatcg taggacccta tctgctcaat gaaagcgccg
     3961 tcccttggag ccctcgaatc ggcaaccacc agacggtaat agggctgctt tttacggcca
     4021 aatcttgcaa gacgaattct gactgccatt cacgtgccac ctcctgttta aagatctgca
     4081 gatcatgttc tttatgggga tcctccgccc tgtcacattc ttacgggcgg gaagcctttc
     4141 gggagtttca tccccctctt gccgggggca aaacgtttca tcagttcgtt catctgctgg
     4201 aactgtttca gcacctggtt caccatctgg accgaagttc cggagcccat ggctatgcgt
     4261 cgccggcggc ctcccttgat gatctgggga tttccgcgct cctctcgggt cattgaaaga
     4321 attattgcct caacccgggc cagtcttcgg ggatccatct ccgcgccctg caacccttta
     4381 atgttggcag atcccggcat catttccaga atcttgtcaa ggggccccat cttgcgaacc
     4441 tgccttatct gcaggagcat gtcgtccatg gtcatcttct tcctgccaac cgacgcggct
     4501 atcttttcca tatccccgtc atcgacggca agtttcactt tttcggcaag gccctgaacg
     4561 tctcccatac cgagaat
//
LOCUS       DAOTIQ010000097         4460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_23178_length_4460_cov_7.060159, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000097 DAOTIQ010000000
VERSION     DAOTIQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23178_length_4460_cov_7.060159"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1587)
                     /locus_tag="P5541_08920"
     CDS             complement(76..1587)
                     /locus_tag="P5541_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRV72145.1"
                     /translation="MKNKRPTALFVVLVTFAILLGASKPFDAVHASPQGFNIKSLVIE
                     GNTIFSEEALVKAPGFDHPIEADMEEIHQMADRITSFYRDRGYPFALAYVPEQSVQDG
                     EVIIKVIEGCWGQITATGDPALIAWAQRFLCGLKPGCVIEEKSLERSILILGDQPGIE
                     VSPLMCPGEKVGTGDLEVQVTEGAKCSGQARLDNHGNRYSGEYRGELTLRMNRAFAIG
                     DELSLRTLYTRENMWLGQLDYSFAIGYSGLRGNVGYAHTAYDLMAPYEGYTGTAKVSK
                     AGLSYPLIRSRKTNLALYVTGQYKDLNNELSGASYEKKTSLSGIAGLQFDHRDSFGGG
                     GITYGDLSVTVGDLDSDNAGAVQGSFTKANLEITRLQNLSSAFTLFVNASGQWSDSDL
                     DSSESFTLGGANGVRAYPQGEASGSEGWLARAELRYFFGGSFAPFIFYDAGHISKDAS
                     TEERGIGGVGLGLRIVRGSQELDITAAWPTHGGPSRSDNTSAPLIWFTLTHSF"
     gene            complement(1577..4039)
                     /locus_tag="P5541_08925"
     CDS             complement(1577..4039)
                     /locus_tag="P5541_08925"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRV72146.1"
                     /translation="MKDIQTLQKHHCFLSIRKALVKPISAGIFSFMLLTITSSTLADT
                     LPTGGNVTAGSGTISQTNTDMTINQASQNMVIDWQGFSIGENNSVTFDQPSSSATALN
                     RVIGSEISHIQGVLKANGRVFLINPSGVLFHNTASVNVGDLVVSTLNMSNEDFMSGNY
                     DFNGNSPGQIINRGDISAADGGYVVFIAAKIENTGTVTANSGGVLFGAGSRVILDLGG
                     PVKLKVEEAAIDALIEQGGAIRADGGLVYISAKAAGDLTFTVINHTGITEARTLSSGE
                     NGEIYLLGDMGNDRISVGGTLDASAPDGGDGGFIETSAAKVKIEEDVHVTTKSEDGET
                     GTWLIDPNDFTIAASGGDMTGATATNALTENNMTIESSSGSQEGNGDIFVNDSITWLE
                     NTLFLKAWNNIEINRELFGSGTAKLALHYGQGTKTGGNTSSYSINAPVNLAEGYKFFT
                     KCGFDGVEKVFLVITSLGVEGESTVTTLQGMKNKLNGYYVLGSDIDAFSTETWNGEEG
                     FEPVGDNLKKFSGILDGLGHTITGLTINRPDSDYVGLFGYMENADIRNLGIEDASVSG
                     RNRVGGLVGYSYDSTISNCYFTGSVKGVNYVGGLAGYSNVSSNISDSFSGGNVEGASY
                     VGGLTGYNRNTSSIDSSFSACLVAGSGTGDYIGGLVGRNTSTVTASYWDTETSGMSDS
                     AGGTGVTTEQMKTMTTFTGWDISEEQDAFSTWYIDPGNDYPRLRAFLSFPETPAGGDS
                     GSNPLTLTPPPDPVLAAIGNSAFSQDPLPGPRFYPPQLNNWPLVGGQGINLPGDVMWY
                     QNWIQEEGLDNRDFEGQDANEE"
BASE COUNT          946 a         1164 c         1248 g         1102 t
ORIGIN      
        1 ctcagaaaga atggttttct ctgtgtggct tcaccctctg tgtcccctgt ggtgagaggg
       61 gccgggaacg tggaatcaaa agctgtgggt cagggtgaac cagatcaggg gggcggaggt
      121 gttgtcggat ctggatggcc cgccgtgggt cggccaggcc gcggtgatgt caagctcctg
      181 ggagccgcgc acaatacgaa ggcccaggcc caccccgcca atgccgcgct cctcggtgga
      241 tgcatccttt gagatgtgtc cggcatcata gaagatgaag ggcgcaaaac tgcctccgaa
      301 gaaatagcga agttctgccc tggcgagcca cccttcgctc ccggatgcct ccccctgcgg
      361 gtatgccctc actccgttgg caccgcccag ggtgaagctc tcggaggagt caaggtcgct
      421 gtcggaccac tgccccgagg cgttcacgaa gagggtaaag gctgaagaaa gattctgcag
      481 tcgtgtgatc tcaaggttag ccttggtgaa ggagccctgg accgctcccg cgttgtctga
      541 atcgagatct cccaccgtta cggagaggtc gccgtatgtg atgcctcctc ctccgaatga
      601 gtcgcgatgg tcgaactgca atcccgcgat gccgctgagg ctcgtcttct tttcgtaact
      661 tgctccggag agttcgttgt tcaggtcctt gtactgcccc gtaacgtaaa gggcgaggtt
      721 ggtctttcgg ctgcggatga gaggatagct cagtcccgcc ttgcttactt tcgccgtccc
      781 cgtgtaccct tcgtagggag ccatgagatc ataggcggta tgggcataac cgacgtttcc
      841 ccttaaaccc gaatagccta tggcaaagga gtagtcgagt tgccccagcc acatgttctc
      901 ccttgtgtag agggtacgga gcgagagttc gtctccgatc gcaaaggcac ggttcatccg
      961 cagggtcagc tctccccggt actcgccgga gtagcggttg ccgtggttgt ccagtcgtgc
     1021 ctgcccggaa cactttgccc cttccgtaac ctgcacttcc aggtcgcccg ttcctacttt
     1081 ttctccggga cacatgaggg gtgatacctc tattcccggc tggtcgccga gtatcaggat
     1141 ggatctttcg aggctttttt cctcgataac gcaccccggc ttgagaccac acaggaagcg
     1201 ctgggcccag gcgatgagtg ccggatcccc cgttgccgtg atctggcccc agcacccttc
     1261 gatcactttg ataatgacct ctccgtcctg aactgactgc tcaggcacat aggccagggc
     1321 aaaagggtat cccctgtcgc ggtagaagga agttatcctg tcggccatct gatgtatctc
     1381 ttccatgtct gcctcgatgg gatgatcaaa accgggcgct tttacaagag cctcttcgct
     1441 gaagatggtg ttgccctcta tcacaaggct tttgatgttg aacccctgag gggatgcatg
     1501 taccgcgtcg aacggttttg atgctccgag aaggatcgca aaggtgacca ggacgacaaa
     1561 gagggcggtt ggtcttttat tcttcattcg catcctggcc ctcgaagtcc ctgttatcca
     1621 ggccttcctc ctggatccaa ttctggtacc acatgacgtc tcccggcagg tttattccct
     1681 gcccgccaac aaggggccag ttgtttaatt ggggtgggta aaagcggggg ccgggaagag
     1741 ggtcctgcga aaaagcgcta ttaccgatgg ccgccaggac agggtctggc gggggtgtca
     1801 gggtcaacgg gttggagccg gagtcgcccc cggcaggagt ttcggggaat gacaaaaatg
     1861 ctctaaggcg cgggtaatca ttgccggggt cgatgtacca ggtggagaag gcatcctgtt
     1921 cttcggatat gtcccagccg gtgaaggtgg tcatggtttt catttgttct gtcgttaccc
     1981 cggttccacc ggcgctgtcg gacatacccg aagtttcggt gtcccagtag gaggcagtga
     2041 cggtgctggt attccggcca accagtccac cgatataatc accagtacca gaaccagcaa
     2101 caagacaggc ggagaaggaa gaatcgatcg aactggtgtt tctgttataa cctgtaaggc
     2161 cacctacata gcttgcgcct tcgacatttc caccggaaaa agaatcagaa atattgctgc
     2221 tgacattgct gtaacccgcg agacctccga cataattgac tcctttgaca ctcccagtga
     2281 aatagcaatt gctgatagta ctgtcatagg aataaccaac gagacctcca actctatttc
     2341 gccccgatac gcttgcatcc tctattccca ggttccggat atccgcattc tccatatagc
     2401 cgaaaaggcc aacataatca gaatcgggtc tgttgatcgt gagccctgtt atggtatggc
     2461 caaggccatc gagaattcct gaaaatttct tcaggttgtc acccaccggt tcaaaccctt
     2521 cttcaccgtt ccaggtttca gtcgaaaatg catcaatatc cgaaccaagg acatagtagc
     2581 cattcagttt attcttcatc ccttgcaggg ttgtgactgt actttcgccc tcaaccccga
     2641 gagatgttat tacaaggaaa actttttcca ctccgtcaaa accgcatttg gtgaaaaatt
     2701 tataaccctc ggccagattg acgggtgcgt tgatgctgta agaagaggtg ttacccccgg
     2761 ttttagtccc ctgtccatag tgcagggcaa gtttcgcggt tccggaacca aagagttcac
     2821 ggtttatttc aatgttgttc catgctttga ggaaaagagt gttttccaac caggttattg
     2881 agtcgttgac gaagatatct ccgttgccct cctggctccc cgacgaggat tctattgtca
     2941 tgttgttttc agtcagcgca ttcgtggccg tcgccccggt catgtcccct ccacttgcgg
     3001 cgatggtgaa atcgttggga tctattagcc atgtccccgt ttcgccatct tcggacttcg
     3061 tggtcacatg aacgtcttcc tctatcttga ccttcgcggc agaggtctct atgaacccgc
     3121 cgtcgccgcc gtcgggggcg gaggcatcaa gggttccgcc tactgagata cggtcgtttc
     3181 ccatgtcccc caggagatat atctcgccat tttcgccact tgagagtgtt ctggcctcgg
     3241 ttatgccggt gtggtttatg acggtgaatg taaggtctcc cgcagccttg gcggaaatgt
     3301 agaccagccc tccatcggcc cttattgccc cgccctgttc tatgagggca tctatggctg
     3361 cttcttccac cttcagtttc accggtcctc caaggtccag gatcaccctg ctcccggcac
     3421 caaaaagcac tccgccgctg ttcgccgtta cggtgcctgt gttctctatc ttcgcggcga
     3481 taaagacgac gtagccgccg tctgcggcac taatatcacc cctgttgatt atctgcccgg
     3541 ggctgttgcc gttgaagtcg tagttgccgc tcatgaagtc ttcgttgctc atgttgaggg
     3601 tggagactac gaggtctccg acgttgacgg atgcggtgtt atggaagaga accccgctcg
     3661 ggttgatgag gaatactctt ccgttggctt tcaggactcc ctggatgtgg gagatctcac
     3721 tgccgatgac gcggttgagg gctgtcgcgc tgcttgaggg ttgatcgaag gtcacgctgt
     3781 tgttctcacc tatggagaat ccctgccagt cgatgaccat gttctgcgag gcctggttta
     3841 tcgtcatgtc cgtattcgtc tggcttatgg ttccgcttcc ggccgtcaca ttgccgccgg
     3901 tggggagggt gtcggcaaga gttgaggatg tgatcgtcaa aagcataaag gaaaagatac
     3961 ccgctgatat gggttttact aaagcctttc ttattgagag gaagcagtga tgtttttgca
     4021 gggtttgaat atctttcaac gtatggcgcg tctcctttgt gtctgatctg cgaaaaaggg
     4081 tcagtgaaag aatcgatgcg agatgcaata caaacttatg ctgaattcat tttaaggttt
     4141 tgggcggagg atttcaatgg gaaaatttat ccagacctct caccgcagag gtcgcagagg
     4201 gtcacaaaga aaggtatttt ggggaggggc aaagctcaac tgcgtaaacg gtgaacggtg
     4261 aacggtgaac gggaaagaca gaatagctag gagactggag actggcatct ggaggcaggg
     4321 agggcaaaag caagggcgtg agaggtgaga ggtgagaagt gagaggaaaa agcaaggaga
     4381 caggagactg gcatctggag acagaaagaa cagataaggt caactttttg ttactcattt
     4441 aggaatggtc tttacgggct
//
LOCUS       DAOTIQ010000098         4430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_23370_length_4430_cov_7.550400, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000098 DAOTIQ010000000
VERSION     DAOTIQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23370_length_4430_cov_7.550400"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1414)
                     /locus_tag="P5541_08930"
     CDS             complement(290..1414)
                     /locus_tag="P5541_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV72147.1"
                     /translation="MKISPFGVEEWMNRWETLAVNNIAETCVDSLTLYELLQMSGRTE
                     EIMAGLLGTRLTYGDIKGSERLRGLISGLYEKIGPESVLVMNGGSAANFLALFTLVEP
                     GDRVISVWPTYQQLTSIPEAFGAKVEILALRPENSWLPDLDELGQMAEGGVDLICINN
                     PNNPTGALMEREMLERIAEIARRHGAWVLCDEAYRGLVHEPGVSSPSMADIYEKGVVT
                     GSFSKVFSLAGIRSGWMAGPVDFIAEAFARRDYTTISCGPLTDPLAVVALENREKIME
                     RNLGIIRKSLSILEEWVDSEDLFHWVKPRAGTTAFLGYDFDMPSDEFCIDLLGTTGTF
                     LLPGKCFGIEKRLRIGYAYGTENLRRGLESVSGWVREGKS"
     gene            1592..2827
                     /locus_tag="P5541_08935"
     CDS             1592..2827
                     /locus_tag="P5541_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV72148.1"
                     /translation="MDWSNRFSDIVRERLEDSAIAVMMNIAAREGALLLAAGEPLEDL
                     CPSKRLKKAFERAHEQESHTWGYSHSRTGVLLLRRWIFERMKSDGILPGWVSPDNIFI
                     TNGSQEGLSLLTESLFDPGDTVMVESPSYPDAFGVFRKEGLELAGVDLLPDGPDIGSM
                     ERLLSDRKVKAFYTIPSFQNPTGFSTSREKKIRILELARKYDFLVIEDEPYRDISFDA
                     APQGSFLAAAGDDDRVLYMGSFSKTISPGLRCGWIVAPEPLVEVLLKLRTAGTLCLPD
                     IVQQAVYEFVSEGDFGEHLKHICEVYRKNRDAMVDFLRRLAGPEGLEINVPSGGFFIW
                     GEIPWIKDIERFGLFAIKEEKVAFIPGTHFFPGKGMGRDSLRFSFARVTPEMAEEGAK
                     RMRDALIKYRMIQEGENRE"
     gene            2824..>4430
                     /locus_tag="P5541_08940"
     CDS             2824..>4430
                     /locus_tag="P5541_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV72149.1"
                     /translation="MKNYSDSELYRLLMDLVRIPSVSPSSERENEIARFIYDRLSGLE
                     YFQKNPSDLRLLPLEGDSLGRHLVFAIVRAPLKTGDTVILAGHMDVVGTEACGSLAPW
                     AFDPEEYTSRIGNTDISPEAREDLDTGEWLFGRGISDMKSGVATGLKLLLDAARDPSS
                     LDSNLAVLVVPDEENNSSGMLSASSYLARFQEQEGLRYLACVELEPTFATGDEAKPSI
                     YLGSIGKINPFFFCAGKETHVGEYYEGFCAAPIISRINLMLDGNPDYADQLFGKAYPP
                     FGCMRQVDLRREYSATIMTRAFAFYSYLTATKLPGEILSGMRKIAEEALKGAIEQYEA
                     NASAFAKRCGFAASPKKWMPTVLTFAELSKMARERLGDGFDRFVKDSLGSAPPGSDER
                     IRAAALVESMVDLCAIPGPMAVIGFLPPWYPHRANLGSNDGEKLMEKVAKEASEIAGS
                     KFGNPLEIRPFFEGVSDLSYCGFQGDSSEMDLFADNMPGWGSPYSLPKDALARLDIPI
                     LNFGPLGKDAHKNTERIHLPYAMDVYP"
BASE COUNT          988 a         1198 c         1246 g          998 t
ORIGIN      
        1 ctggaaacag gagactggaa gggcaaaaaa gccgtaagac gtgagacgtg agacgtgaga
       61 cgaatggaaa aacaagggct tgtcattctg agcgaagcga agaatctgtt ttaggttccg
      121 ggaatcgtga atcgaaaaag tcaaaggcaa acccagatcc tcatccgcca gctggcggac
      181 tcctcaggat gacagcaacc cgtcaagggc gtcaacggtg aacggtgaac ggtgaacggt
      241 gaacggtgag gcacctgtcg cctgtctcca gtctccttga tctttcgatt cacgatttac
      301 cttccctgac ccaccccgag acactttcca gcccccgtct caggttctcc gttccgtagg
      361 cgtagccgat gcgcagccgc ttctctatgc cgaagcactt ccccgggagc aggaaggtcc
      421 ccgttgttcc cagcaggtcg atgcagaatt cgtcggaggg catatcaaaa tcgtagccca
      481 ggaaagccgt cgttcccgcg cggggcttga cccagtggaa aagatcctcc gagtccaccc
      541 attcttccag tatggacagg ctcttcctaa taatgccgag gttcctttcc attatctttt
      601 cccggttctc cagcgctacc acggcgaggg gatccgtgag agggccgcag ctgatggtcg
      661 tataatcacg cctggcaaag gcctcggcga taaaatcaac cgggccggcc atccagccgc
      721 ttctaatccc cgccaatgaa aaaaccttgg agaaacttcc cgtaacgacg cccttttcgt
      781 atatgtccgc catggaaggc gacgagactc caggttcgtg aaccagcccg cggtaagcct
      841 cgtcacagag gacccaggcc ccgtgcctgc gcgctatctc cgctattctc tccagcattt
      901 ccctctccat cagagccccg gtcggattgt tgggattgtt gatgcagatg aggtccacgc
      961 cgccttcggc catctgcccc aactcatcaa ggtcgggaag ccacgaattt tccggtctaa
     1021 gggcaaggat ctccaccttg gccccaaaag cctcgggaat gcttgtcagt tgctgatagg
     1081 tcggccagac gctgatgacc ctgtcccccg gttccaccag ggtaaaaagg gccaggaaat
     1141 ttgctgccga acccccgttc atcacaagga ccgactctgg gccgatcttt tcgtacaggc
     1201 cggagatcag acccctcagt ctttcgctcc cctttatgtc tccataggtg agccttgttc
     1261 ccaggaggcc cgccattatc tcctccgtcc ggccggacat ctggagcagt tcgtaaaggg
     1321 tcagtgagtc cacacatgtt tcggctatgt tgttgaccgc cagggtctcc caccggttca
     1381 tccactcttc cacgccgaag gggcttatct tcatcgatat cactcctgac tgaatcgttt
     1441 gttcgacttt ttcagtatgc acggttatga agaataagtg aaggggtcct gttttgagta
     1501 tgtcaggcag actgatacaa tcatgcatgt tgaattataa taatccccct ttactggggg
     1561 aattgtttct tccccgggag ggaatcagcc aatggactgg tctaaccgct tcagcgacat
     1621 tgtcagagaa cgtcttgagg attccgccat cgcagtaatg atgaatatag cggcccggga
     1681 aggagccctt cttcttgctg ccggtgagcc gctggaggat ctgtgcccct cgaaaagact
     1741 caaaaaggcc tttgaaagag cgcatgaaca ggaatctcac acctgggggt acagccattc
     1801 cagaacgggt gtgcttctcc tgcgaaggtg gatcttcgag cggatgaaga gtgacggcat
     1861 ccttcctggc tgggtatcac cggacaacat attcatcacc aatggttccc aggagggtct
     1921 ttctcttctc accgaaagcc tttttgatcc gggtgatact gtcatggttg aatcgccatc
     1981 ctaccctgat gctttcggtg tgttccgtaa ggaggggctc gaacttgcgg gagtcgacct
     2041 gctccctgac gggccggata tcggatcgat ggaaaggctc ctctccgatc ggaaagtgaa
     2101 agccttctat acgatccctt cctttcagaa cccgaccggt ttttctacga gccgggagaa
     2161 gaagatccgc atcctggaac ttgccaggaa atacgacttc ctcgttatcg aggacgaacc
     2221 ctacagggac atttccttcg acgcggctcc gcagggaagt ttcctggctg ccgcgggtga
     2281 tgacgacagg gtcctgtaca tggggagttt ttccaagacc atctccccgg gtctgaggtg
     2341 cgggtggatc gtggcccccg aaccacttgt ggaggtcctt ctaaaactgc gaaccgcggg
     2401 aaccctctgc ctgcccgata tagttcagca ggcggtttat gagtttgttt cggagggcga
     2461 ttttggagaa catctgaaac atatttgtga agtctacaga aagaaccggg atgccatggt
     2521 tgatttcctg aggagacttg ccggaccaga gggactggag ataaacgtcc cctcgggggg
     2581 attctttatc tggggggaaa tcccatggat aaaagatata gagagatttg gcctctttgc
     2641 tatcaaggag gaaaaggttg cctttattcc cggcactcac tttttccccg ggaagggcat
     2701 ggggagagac tctctccggt tctcctttgc cagagtaacg ccggaaatgg ccgaagaggg
     2761 cgcgaagagg atgagagatg cgctgataaa gtacaggatg atccaggaag gagaaaacag
     2821 agaatgaaga actattccga cagtgaactg taccgtctct tgatggatct ggtaaggata
     2881 ccgagcgtct ctccttcatc ggagcgggag aacgagatcg cccgcttcat ttatgacagg
     2941 ctttcgggcc ttgagtattt tcagaagaac ccttctgacc ttcggttgct tccgctggag
     3001 ggggactccc ttgggagaca cctggtattt gccattgtca gggctcctct gaagactggc
     3061 gacacggtga tactcgccgg gcacatggat gtggtcggta ccgaagcctg cggttctctg
     3121 gccccatggg ccttcgatcc tgaggagtac accagcagga taggaaacac ggatatttct
     3181 cccgaggcaa gagaagacct cgataccggc gagtggctct tcgggcgtgg tatttccgac
     3241 atgaagagcg gtgtcgctac aggattgaaa ctgctcttgg acgcggcgcg cgatccgtca
     3301 tcccttgatt cgaacctggc ggttttggta gtcccggacg aagagaacaa ctcctcggga
     3361 atgctttctg cctcgtcata tctggcccgc ttccaggagc aggaaggact ccgttacctg
     3421 gcctgcgtgg agctggagcc cacattcgct acgggagacg aagcgaagcc gtcgatatat
     3481 ctcggaagca taggcaagat aaaccccttc ttcttctgcg cgggcaagga gacccacgtc
     3541 ggggagtatt acgagggttt ctgcgccgcg ccgataatct cccggataaa cctcatgctg
     3601 gacggcaatc cggattatgc ggatcaactc ttcggcaagg cctacccgcc ttttggctgc
     3661 atgcgccagg tggatctgcg ccgcgagtat tcggccacta taatgaccag ggcctttgcc
     3721 ttttacagtt atctgacagc gacaaaactg cctggcgaga tcctgtcggg gatgagaaag
     3781 attgctgaag aagccctgaa gggggctata gagcagtacg aggccaatgc gtcggctttc
     3841 gcgaagagat gcggattcgc ggcttcgcca aagaaatgga tgcccacggt tctgaccttc
     3901 gccgaactgt ctaaaatggc cagagagagg ttgggcgacg ggtttgaccg gttcgtaaag
     3961 gattcgttgg gatccgcccc ccctggctcc gacgaaagga tccgcgcggc cgcccttgtg
     4021 gaatctatgg tggatctctg tgccataccg ggtcctatgg cggtcatagg gttcctcccc
     4081 ccgtggtacc ctcacagggc caacctgggc agtaacgatg gggagaagtt gatggagaag
     4141 gtggcaaagg aggcatcgga aattgccggc agcaagtttg gcaatcccct ggaaatcagg
     4201 cccttctttg agggcgtctc ggatttgagt tactgcgggt tccagggtga cagcagtgag
     4261 atggacctgt tcgctgacaa tatgccggga tggggttctc catacagcct gccgaaagat
     4321 gccctggcac ggcttgatat cccgatattg aacttcggcc ccctgggaaa agatgcccac
     4381 aagaacaccg aacgcatcca tctaccctac gcaatggatg tttatccgga
//
LOCUS       DAOTIQ010000099         4421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_23437_length_4421_cov_7.410673, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000099 DAOTIQ010000000
VERSION     DAOTIQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23437_length_4421_cov_7.410673"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5541_08945"
     CDS             <1..200
                     /locus_tag="P5541_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV72150.1"
                     /translation="EAPLLVIHSPEDEIVPFSEGRKLFDIAREPKEFLEISGDHNGGF
                     IISGNTYRKGLGDFLSSLEKQ"
     gene            complement(262..1653)
                     /locus_tag="P5541_08950"
     CDS             complement(262..1653)
                     /locus_tag="P5541_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRV72151.1"
                     /translation="MDWKALAKFTSKNISNSCEIIQRDEKFIPGAVAIKYFPMALEKG
                     EGSLVWDADGNRYIDFLTSAAVYNTGHCHPAIVEAVREQVGKLQNYTVVYFYNDRQVR
                     LAETLVGTTPGTYPKKAAFGFSGSDGVDAAVRSARSFTGRKKIISFKDPYHGMTTVSL
                     SVTGIIGSDVKECVCPDRDVLFAEYPDPYRNSWGIDGYADPEALTGAAMEAIGKMLEQ
                     NRGEIAAVLIEPAQGDGGMIFPPASFMKQLREVTEREGIVFIDEEVQTGMGRSGRFWA
                     IEHFDVVPDLIVSAKALGGGVPISAVVGKADILDSVPVPLLAYTHTGHAVNCAAAEAT
                     IDVVKKEKLAEQAEEKGRRLTEWFRERSDRFPFIGDIRQKGLLMGVEIVSDRTNKTPD
                     KAMALKISWAAWEKGLILITFGKNGNVLRIAPPLNITEELFQEALEIMNAALEDAATG
                     RVSDDILPHLKGW"
     gene            complement(1678..2823)
                     /locus_tag="P5541_08955"
     CDS             complement(1678..2823)
                     /locus_tag="P5541_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72152.1"
                     /translation="MSAVQENGQGFFSRMMASSSYKKYILPGLISQSVIIAGGYGTGR
                     ELVEYFVSYGSLGGILGMVLVTITLWSLVFAVSYEFARTFQVYDYRGFFKELLGPGWV
                     LYEACYVVLLLIVLGVVGATSGSIFMQSFGLPPMVGAGLFLAGVATLTFFGSRVIEVA
                     MSWWSYLLYAVFLVFLLVGLSQVGGQISANLASGEIKEGWALGGFKYAFYNLGTFPAI
                     LFALNYIESRKEAVISGIMTSIITILPGLFLYLVIMGFYPGILDVELPAYTILGQIAP
                     WLLPVYMVVLFGTMIETGAGFIHAVNERINSWMVDRKGKGLTRANRGVLGGIMALIGL
                     GVASFGLIGLIAKGYGTISWGFFILHGVALFTVGIYKIHKKNSAQTR"
     gene            complement(3231..4331)
                     /locus_tag="P5541_08960"
     CDS             complement(3231..4331)
                     /locus_tag="P5541_08960"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV72153.1"
                     /translation="MNWKRTMPPAVRDMEFAEYATSREERPVAVDCSLGTNPLGSPES
                     SGRICRSASLPDPCPYPGDDLPFREGLSKYWGGSFSPEEVIPGTGSIGLIISVARTFC
                     APGSIVLGAVPQFPDGPMHFQFSGAAYRAVRLAPPLFRLEIGALLSAIKGDESIVYLD
                     RPHNPTGQAPPLKDLRPLADTCDESGTLLVVDEAYGDFIPPEESAIQLNNRSVIVLRS
                     FSKGKGLAGIRTGYAIVRDPEAHRFMRKVAPPFSITSTALEMAVASLEDAEYTARSVE
                     ITGSIKRKVLRAIEGTPGVICAETHPDVPILLVSTLSKNANLYETLMAAGIRSEAGTC
                     FEGLGPESVRLRIPSPGDLEIFLERWSSAFGK"
BASE COUNT         1037 a         1224 c         1172 g          988 t
ORIGIN      
        1 ttgaggctcc tcttcttgtg atccacagcc ccgaagacga aatagtccct ttttcagagg
       61 gccgaaaact tttcgatatt gccagagagc ctaaagaatt cctggaaata tccggggacc
      121 acaacggggg gttcataata tccggtaata cctacagaaa gggtctgggt gattttctct
      181 ccagcctgga gaaacaatag gaaaggcccc ggggtgcgct ccccggggcc tttcctcaga
      241 agagaaggat tggatctgtt gctaccagcc tttgaggtgc ggcaggatgt cgtcggacac
      301 cctccctgtt gccgcatcct ccagggcggc attcatgatt tccagtgctt cctggaaaag
      361 ttcctcggtg atgttgagag ggggggctat tcggagaacg ttaccgttct ttccgaaggt
      421 tattagaata agccccttct cccaggcggc ccaggaaatt ttaagggcca ttgccttgtc
      481 gggtgtttta ttggtcctgt cggagactat ctcgacaccc atgagaagcc ccttctgacg
      541 gatgtccccg ataaagggga aacgatctga cctttcacgg aaccattcag tcaggcgtct
      601 gcctttttct tcggcctgct ccgcaagttt ttctttcttt acgacatcta tggtggcttc
      661 ggccgcggcg caattgaccg catgaccggt gtgggtgtag gcaagaagcg gtacgggtac
      721 cgagtcaaga atatcggctt ttccgacaac cgccgaaatg ggaaccccgc caccgagcgc
      781 ctttgcggaa acgatcaggt ccggaacaac gtcgaagtgc tcgatagccc agaatcttcc
      841 gcttcggccc atacctgtct ggacctcttc atcgatgaat acgatcccct cgcgttccgt
      901 aacctcccgg agctgtttca tgaaactggc cggcggaaag atcattccac catcgccctg
      961 ggcaggttcg ataagaaccg cggctatttc ccccctgttt tgctccagca tcttgccgat
     1021 agcctccatt gcggcgccgg taagcgcctc ggggtcggcg tagccgtcaa ttccccagga
     1081 gttcctgtag gggtccgggt attccgcgaa aagaacgtcc ctgtccgggc agacgcattc
     1141 cttgacatcg gaacctatga tccctgtgac ggaaagggat accgtggtca tcccgtggta
     1201 gggatccttg aatgatatga tcttttttcg tccggtgaag gatcgggccg aacggaccgc
     1261 tgcatcgaca ccgtctgatc cggaaaaacc gaaggcagcc ttctttggat atgttcccgg
     1321 agttgtccca acaagtgttt cagccagcct gacctgcctg tcattgtaga aataaacgac
     1381 ggtgtagttc tgaagtttcc cgacctgttc ccttactgcc tcgactatgg ccgggtggca
     1441 atgccctgtg ttgtagaccg ccgcgcttgt gagaaagtcg atataccggt tgccgtcggc
     1501 atcccagacg agcgaacctt cgcccttctc cagtgccatc ggaaaatact tgatggccac
     1561 cgcgccagga atgaacttct catcccgctg gattatttcg caactattgc tgatattctt
     1621 gctggtgaac ttcgcgagag ccttccagtc catgaagtgt tcctccgttt ttcggaatta
     1681 tcgggtctgg gcagaattct ttttgtggat cttgtaaata cctacagtga agagcgccac
     1741 cccatgcagg atgaagaatc cccagctgat ggtgccgtac cccttcgcta taaggccgat
     1801 caggccaaaa gaagcaactc ccagaccgat gagagccatt ataccgccaa gtaccccacg
     1861 gttcgcgcgg gtgagacctt tccccttgcg atcgaccatc caggagttga tccgctcgtt
     1921 gaccgcgtgg atgaaacccg cccctgtttc tatcatcgtg ccgaaaagga ccaccatgta
     1981 gacgggaagg agccatggag cgatctgtcc gagtatggta tatgcgggaa gttcgacatc
     2041 aagaataccc gggtagaaac ccatgattac aaggtagagg aaaaggccgg gcaggatcgt
     2101 aatgatggag gtcattatac ccgagataac ggcttctttc ctgctctcga tatagttcag
     2161 ggcaaagagt atcgccggga aggtccccag gttgtagaag gcgtatttga accctccaag
     2221 ggcccaccct tccttaattt cgcccgatgc caggttcgcg ctgatctgcc ctccgacctg
     2281 cgaaagaccc acgagcagga agacaagaaa aacggcgtaa agcaggtagg accaccatga
     2341 catggcgacc tcgatgaccc tgcttccaaa aaaagtcagt gttgccacgc cggcaaggaa
     2401 aagacctgcc ccgaccatgg gaggaaggcc gaaagactgc atgaaaatac ttcccgaggt
     2461 tgctccgaca acaccaagaa caatgagaag aaggacaaca taacaagcct cgtaaagaac
     2521 ccaccccggg cccagaagtt ccttgaagaa gccgcggtag tcataaacct ggaaagttct
     2581 ggcaaattca taacttacgg cgaagaccag ggaccacagg gtgatggtga caagaaccat
     2641 acccaaaatg cccccgaggc tgccgtaact tacgaaatac tcgaccagtt cccggccggt
     2701 accatagccg ccggcaataa tgacggattg tgaaataagt cccggaagaa tgtacttttt
     2761 gtaagatgaa gaggccatca tcctgctgaa gaaaccctgt ccgttttcct gaactgcact
     2821 catctctagc aacacctttc tctttcgtct ttgttgcgtc tctcccgttg atacaggtac
     2881 ccgatcctgg aattgcaaaa aaccggtttt atatctcctc ccctcaaaaa aggggcccct
     2941 tccttctgga aggggcccct gatctctctg gctcgttgcg gcagggtaaa cctcagccgc
     3001 gccaggaggg gaaccccaag gcgtaatagc ggataatagc gaaagtaaaa tatgcagaca
     3061 ctatatcgtc agccctcaag ggtaaccctc cctctaaata agttatgcat tatgaaaagc
     3121 ccgcgtttca gggagtctat aacacctgtc ctgaaatgtc aacaatggcc ggacacaatt
     3181 gtggccggac agcgataatg tcacccttta aggggacagg gaaaatgtca ttatttacca
     3241 aaggcactac tccatcgctc caggaatatc tccaggtcgc ccggggaggg tatgcgcaaa
     3301 cggacgcttt ccggccccag gccctcgaag caggtcccgg cttccgatct tatgcccgcc
     3361 gccataaggg tttcgtaaag attggcattc ttgctgaggg ttgacaccag cagtatcggg
     3421 acatcggggt gtgtttcagc gcatattact ccaggagtcc cctctattgc ccgcagaacc
     3481 tttctcttga tactgcccgt gatctcgaca gaccttgcgg tgtactctgc atcctcaaga
     3541 gaggctacgg ccatctcaag agcggtgctg gtgatagaga aagggggagc cactttccgc
     3601 atgaaccggt gcgcttcggg gtcccggact atggcgtacc cggttctgat ccctgccagc
     3661 ccctttcctt tcgagaaaga gcgcagaacg ataaccgatc tgttattcag ctggatcgcc
     3721 gattcttccg gagggataaa gtccccgtag gcttcatcaa cgacaaggag ggtacctgac
     3781 tcatcacatg tatcggcgag aggcctgagg tctttcaggg gcggtgcctg gcctgtgggg
     3841 ttatgaggcc tgtcgaggta gacaatggac tcatcgcctt ttatagcgga aagaagggca
     3901 cctatctcca gcctgaacag gggcggggcc agcctgacgg ccctgtatgc ggccccggag
     3961 aactggaaat gcattggccc gtcagggaac tggggaacag ccccaaggac gatcgatccc
     4021 ggagcgcaga aggttctggc gacggaaatt atcagcccga tggagcccgt accggggatc
     4081 acctcctccg gggaaaaaga gcctccccag tactttgaga ggccttccct gaagggaaga
     4141 tcatcaccag gatacgggca agggtctggc agagaggcgg agcggcatat cctgcctgaa
     4201 gattccggag aacccagggg atttgttcca agggaacagt ctacggccac ggggcgctct
     4261 tccctggatg ttgcgtattc tgcaaattcc atgtccctga cggcaggggg cattgttctt
     4321 ttccagttca tagttccacc ttcccttacc cggagttggg agcaatgata gcacacttgc
     4381 ccttgatctt ccgcttcaca ccgttcaccg ttcaccgttg a
//
LOCUS       DAOTIQ010000100         4407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_23515_length_4407_cov_7.460708, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000100 DAOTIQ010000000
VERSION     DAOTIQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23515_length_4407_cov_7.460708"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..587
                     /locus_tag="P5541_08965"
     CDS             225..587
                     /locus_tag="P5541_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRV72154.1"
                     /translation="MSNYHEPVEELAAEDRDISRALNSLKEEIEAVDWYHQRAAATKD
                     ETIRAVIVHNRDEEIEHAAMMLEWLRRNMPEFDHALRTFLFTEAPITEVEEAAMAGEQ
                     APGKASSDSSLGIGSLKG"
     gene            615..1415
                     /locus_tag="P5541_08970"
     CDS             615..1415
                     /locus_tag="P5541_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HRV72155.1"
                     /translation="MDILKRSISPISAAAWEEIDEQAVKVLKSRLSGRKFVDVAGPFG
                     WAHASVPTGRLDVSKAEGKGEVGWGVHLVQPLVESRVSFEMGVWELDNVARGAKDLDL
                     DPLIEAAEKMASFEENAIYHGFEPGCIVGLAQSGEGQQVSVSTDNPREIMAGLSEGLA
                     FFQKNSIEGPYTLVAGPRLWQILDVFGEGYPLKKRVSSMFDGGMILAPELEGGFLVST
                     RGGDFELTLGQDFSIGYESTVGDKVRLFMAESFTFRVIEPGAVVPLEI"
     gene            1779..1994
                     /locus_tag="P5541_08975"
     CDS             1779..1994
                     /locus_tag="P5541_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HRV72156.1"
                     /translation="MAEYTNTSCRMSLKLQIGTSTEGRPILRSISLRDLSTDALANDV
                     ADASAALGALLEFPVVETSKIDTDLVA"
     gene            2029..2241
                     /locus_tag="P5541_08980"
     CDS             2029..2241
                     /locus_tag="P5541_08980"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HRV72157.1"
                     /translation="MAQTLRMVFGTAAGDPWAMTLRYPKNDLTAQNVTDAMQAVIDAD
                     VFAAGLTGIVSAQIIDREVTDLVSNG"
     gene            2305..2454
                     /locus_tag="P5541_08985"
     CDS             2305..2454
                     /locus_tag="P5541_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HRV72158.1"
                     /translation="MEDFLASAIQSGFAVAVAGYLLVRMESRLEELTKAIRELQLAIV
                     NRGGL"
     gene            2457..2879
                     /locus_tag="P5541_08990"
     CDS             2457..2879
                     /locus_tag="P5541_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HRV72159.1"
                     /translation="MKTNNFFVSPHFRLVEFQCRCCGCVKLHPELLAKLEILREAWKS
                     PLLLTSGYRCPNHNKTVQGVEGSLHLLGQAADIVVFSEDQPRFIEEAKMAGFDQAIPY
                     GKRNFVHLGIFGQDERVILPVNQHKWETAAVVHAGAGA"
     gene            2860..3339
                     /locus_tag="P5541_08995"
     CDS             2860..3339
                     /locus_tag="P5541_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV72160.1"
                     /translation="MPEREHEEILTAFRPLVMATARRYCGNGAAFDDLVQEGYLAMLE
                     LLPRCKNRKYLAKYLKDRLPARVRTAARRAWRTNDSEEELDPETCKGCSFTPEIPWLQ
                     WVAEELLGSRDSQIVRLIAEGYTQKDIAETMLLTQQAVSHRVGQIRRVMNREIETRN"
     gene            complement(3599..4114)
                     /locus_tag="P5541_09000"
     CDS             complement(3599..4114)
                     /locus_tag="P5541_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRV72161.1"
                     /translation="MKGRVLVAYETSKGSTAEIAEVIAAEIRTAGWDAEAVDVKDSPL
                     PADYSFVVVGGPIYLGSIKGVKGFVERHKAVLQERLIGAFAVGMSFAVEDEEKQAAGR
                     KALDEAIAPLEVAHLGYFAGKIDMEKLSFLEKMAVKMVKSPVGDFRDWDAVKGWAQEV
                     LMAVNRESGKP"
BASE COUNT         1068 a         1247 c         1171 g          921 t
ORIGIN      
        1 ccacagagaa aacctttctt tgtgagaacc cggaacctaa gattttcctg cctccagcct
       61 cccgactcct gccgcctcgc cttttcgtct cacgtctcgc ggctcttgcc cttcccttct
      121 tgtccccgcg gaaaggtgat gataacatgt tattcagtga aaggctggga agaggttaaa
      181 gccccggttt cattttttaa ataacgaaag ggagtgagtg cctcatgtcg aactaccatg
      241 aaccggtcga ggaacttgcg gcagaggaca gggacatcag cagggccctg aatagcctca
      301 aggaggaaat agaagcggtg gactggtatc accagcgtgc cgccgcgaca aaggacgaga
      361 ctatccgtgc cgtaatagtt cacaacaggg acgaggagat cgagcacgct gccatgatgc
      421 tcgaatggct ccggcggaac atgccggaat ttgaccatgc tctcagaacc tttcttttca
      481 ctgaagctcc catcaccgag gtagaagagg cagccatggc gggagagcag gcccctggca
      541 aggcttcttc ggacagcagc ctcggcatcg gcagccttaa aggttaggga caggacagga
      601 gggaggatat aaatatggac attctgaagc gatccatttc ccccataagt gccgcggcat
      661 gggaagagat cgacgaacag gcggtaaagg tcttaaagag caggctttcc ggcaggaaat
      721 tcgtggatgt tgccggacca tttggttggg cccatgcttc cgtaccgacg ggccgccttg
      781 atgtttcaaa ggcggagggc aagggtgaag tgggctgggg tgtgcatctt gtgcagcccc
      841 tggttgagtc ccgtgtctcc ttcgagatgg gcgtatggga actggacaat gtcgcaagag
      901 gggcaaagga cctggatctc gatccgctca ttgaggctgc cgaaaagatg gcatcctttg
      961 aagagaacgc aatatatcac ggttttgaac cgggctgcat agtcggactt gcccagtctg
     1021 gtgaaggcca gcaggtctcc gtctcaaccg ataatcccag ggagatcatg gccggtcttt
     1081 ccgagggact tgcgtttttc cagaaaaact ccatcgaggg tccctatacg cttgtcgccg
     1141 ggcccaggct ctggcagatc cttgacgtct tcggcgaggg ctatcccctg aagaagaggg
     1201 tctcctccat gttcgatggc ggcatgatcc ttgctcccga actggaaggg ggtttccttg
     1261 tctcaacgag gggcggcgat tttgaactta ctctgggaca ggacttctcc ataggctacg
     1321 agtccaccgt gggtgacaag gtgcggctct tcatggccga atcctttacc ttcagggtga
     1381 tagaacctgg agcagtggtt cctctggaga tttgaggctg taactcgtaa cgcgtgactc
     1441 ccaagtcgat aatcgaaaac agcaaaaagg gccggaagcg atcatcgctt ccggcccttt
     1501 gcttgcggcc cttctcacca ctgagaaaac ccccctcacc gttcaccgtt caccgtttac
     1561 gcagttgttt caggttttcc tgcctccagt ctcccgactc ctgccgcctc gccttttcgc
     1621 ctcacgtctc acgcccttgc ctttgcccct cctgcttccc gtctcctgtc gcctgtttcc
     1681 ttcctttagg tttgttccct atttgtatat ttcaacattt aatggcactc ttatagttgt
     1741 cagacagcaa aaccaaaaac agagaggagg tgaagtccat ggccgaatac acaaacacat
     1801 cctgccgaat gagtctcaag ctccagatcg gaaccagcac cgagggacgc cccatactcc
     1861 ggagcatatc cctcagggac ctttccaccg acgcactggc caatgatgtg gccgacgcat
     1921 cagctgctct cggtgccctg ctcgagttcc ctgtcgtcga gaccagcaag atcgacaccg
     1981 atctcgtagc ctagaaatag cactaaataa gagaagggag gtgaaaaaat ggcgcagact
     2041 ctgagaatgg ttttcggaac cgctgcgggt gatccctggg caatgaccct gaggtatccc
     2101 aagaatgacc ttacggcaca gaacgtgacc gatgcaatgc aggcggtgat cgacgcggat
     2161 gtatttgctg ccggtcttac gggcatcgtt tccgcccaga tcatagaccg tgaggtcacc
     2221 gacctcgtca gcaacggctg atccatatag atagaacggg ggggcaaaaa acgcccccca
     2281 aaaacagaca aggagggtga atatatggag gactttctgg cttcggccat ccagagcggg
     2341 tttgcggtag ccgttgcggg ctatcttctc gtcaggatgg aaagccgcct tgaagaactg
     2401 acaaaagcga taagggaact ccagctggcg atagtcaaca ggggagggct ctaaacatga
     2461 aaacgaacaa cttttttgtc tccccacact tccggctcgt ggagtttcag tgcagatgct
     2521 gtggatgcgt aaagcttcac cccgaactgc tggcaaaact cgaaatactc agggaggcct
     2581 ggaaaagccc ccttctcctg accagcggat atagatgccc gaatcacaac aagacagtcc
     2641 agggggtaga aggcagtctc cacctcctgg gccaggcagc tgacatagtg gtattcagtg
     2701 aagaccagcc ccgattcatc gaagaggcaa aaatggcggg attcgaccag gcaatacctt
     2761 acggaaaaag aaacttcgta catctgggaa tattcgggca ggacgaaaga gtgatcctcc
     2821 cggtgaacca gcataaatgg gaaacggcgg ccgttgtcca tgccggagcg ggagcatgag
     2881 gagatactga cagcgttccg gccgctggtc atggcaacgg ccaggcgcta ctgcggcaac
     2941 ggtgcggcat tcgacgacct ggtgcaggag ggataccttg cgatgctcga gctcctgccc
     3001 aggtgcaaaa acaggaaata ccttgcgaaa tacctcaagg acagactccc cgcaagggta
     3061 aggacggcgg cccggagggc ctggaggaca aacgatagcg aggaggaact ggatcctgaa
     3121 acctgcaagg ggtgttcctt caccccggag ataccctggt tgcagtgggt tgccgaagag
     3181 cttctcggca gcagggattc acagatagtc cggctcatag cagagggcta tacacaaaaa
     3241 gacatcgccg aaacaatgct cctcacccag caggccgtaa gccatcgtgt ggggcaaata
     3301 aggagagtca tgaacagaga aatagaaacg cgcaactgaa aaaaccagga ggccgaccta
     3361 cggcctcctg gtttttagtc ccccgtcacg gattttctca ccccgttcac cgttcaccgt
     3421 ttaccgttca ccgtttacgc atttgatttg attctgtcat cctgaggagc gaagcgaccg
     3481 aaggatctga ccctaagttc ctctcaccac agtggacgca gagggtggtg ccccgcagag
     3541 aaaacctgga tcatttttgt gggtttaaaa ctcccagtca cgagttacgc gttacgcgtc
     3601 acggttttcc cgattcccga ttcacggcca ttaaaacctc ctgcgcccag cctttcacgg
     3661 catcccagtc gcggaaatcc ccaacggggc tttttaccat ctttacggcc atcttttcca
     3721 ggaacgaaag cttttccatg tcgatcttcc cggcgaaata ccccaggtgc gcaacctcaa
     3781 gcggggctat cgcctcgtca agggccttgc gccccgcggc ctgtttttct tcgtcctcga
     3841 cagcaaagga catccccacg gcaaaggcac cgatcaaacg ttcctgcagg accgccttgt
     3901 gcctttcgac gaaacccttc actcccttga tactgcccag atagatgggg cccccgacga
     3961 caacaaagct gtaatccgcg gggaggggcg aatcctttac atccaccgct tcggcatccc
     4021 agcctgccgt ccggatctct gcggctatca cttccgcgat ctcggccgtc gatcccttgc
     4081 tcgtctcgta ggcaaccaga acccgtcctt tcaaatcttc caccccttcc gccccggccg
     4141 cagaaacgca cccggccaaa aggcatgcaa ataaaaagca cagggacagg aggctccaga
     4201 atctcttccc gcaccctgca acatgtccat tcatctctcg gttcaccccc acgtggtttg
     4261 ctccaaaagg ccctgaaaat aatgcaaata tagagaatta taagcagaaa agctgccagg
     4321 acaaggcaaa aattttctca ccgcagtgga cccagagggt gaaaccacac agagaaaacc
     4381 tttctttgtg agaacccgga acctaag
//
LOCUS       DAOTIQ010000101         4298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_24327_length_4298_cov_7.368136, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000101 DAOTIQ010000000
VERSION     DAOTIQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24327_length_4298_cov_7.368136"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2318
                     /locus_tag="P5541_09005"
     CDS             <1..2318
                     /locus_tag="P5541_09005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV72162.1"
                     /translation="LWITDYDSLKEDIEGIVKFSYIQRVEVAEEGGRSFFSGVEVDPS
                     FEVRSMDLVYDYRGSLRKIGDVSLFIDIGQMHTDLGKNVLILLYIQLFATLVISLIIG
                     GIFHFMIGRHLRGLADFMGKDDPSTGTSSFMLDGNFSRKNELGMLVDHFNGMRERIRN
                     YVGEIREWRDLMHYVIQYDPSAIMVLDRDMRFIFVSDKLLENNGLTLDEVIGKSFSEV
                     LPHLEGKWTDILSQALQGEILGSADDVILRKDGRIDNLRWYCRPWFKADGQIEGTVYY
                     SEVISERKKMERALFVEKELFRTTLFSVGDGFISTDNDGKVLLMNRVARELTGWDPDE
                     ARGKAFSEVLNIVNEKTREKAPDPVKEVLYTGETFEMAQNCLLVSKSGKEIPIEDSAA
                     PVRDIDGDITGVVVVFRDCTDKRKRQQEIEYLSYHDQLTGLYNRRYFEEEVNRLDVPR
                     NLPLAMAMLDVNGLKLFNDAFGHLMGDRLLKRVAEVLKNECRADDIIARTGGDEFVVL
                     LPKTASDDAVAIMDRVSEVIKGEKIEDIPLSISWGVDVKTDRGESIEKVFEKAEDSMY
                     RNKISEKTSYHHRSVQLILETLFARSEFEKSHSENVGALSERIGKAMGLSRVEAGELR
                     TAGVIHDIGKIATPGGVLGKASALEEEEWDEIRKHPETGFSILSTVSEYGPLAEVVFS
                     HHERWDGTGYPRGLRGENIPLFSRIIAVAEAWDAMIAGRPWKRSLSEKEAITELESCA
                     GTQFDPDVVKVFFEIISSPESGEVPDIANGD"
     gene            2353..>4298
                     /locus_tag="P5541_09010"
     CDS             2353..>4298
                     /locus_tag="P5541_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV72163.1"
                     /translation="MSKKNEPGKGRDKVVLFEDFFTSMLNSVTESAFLITQDGIVLAA
                     NETVAKRLGFSSGGDLVGRNMYDLLPPEVAESRRSKVQEVVLTGEPVQFEDVRSGRWI
                     HNSIYPVYDSTGGVSRLSVFAIDITRQKAQEERLSLLGEMVDSAPASITIHDTGGNFI
                     YVNRETLRLHGYTEEEFMSLNLKDLDVPESVELLEERFRMIAEKGEMSFESAHFHKDG
                     SVIPVLVMAKNLDWKGTPAVMSIATDIRESKKAEQALRENEELIMAVMDNLPLGVAVN
                     SLDPAVEFSYMNDRFWKTYKTSREELSGPDAFWSAVYKDPEFREKIRERVLKDCESGD
                     PGKMQWDDVPVTREGETFFVSARNTPVPGSSLMVSAVWDVTEYKKTEDQLRRASERLQ
                     AIYDHSPLLISEFDLEGRYLLVNRAISELFGLSPRELEGRTFSELLPEEVSEIFMRRT
                     RHVWDSQKPLATEDTVDIGGVKRHFLTMLFPLYEKDGHINSIGSIAHDITDRKEADDS
                     LRDALGRLRRVTGSVIQVIVMAVESRDPYTAGHQRRVGDLARAIATEMGLTDHDVEGI
                     RIAGVIHDLGKISVPSEILSKPRILNDTEFAIVKEHPQKGYDILKDVDFDWPVAEMVL
                     QHHERLDGSGYPRGLKDGEILLQAR"
BASE COUNT         1091 a         1017 c         1281 g          909 t
ORIGIN      
        1 acctgtggat aacagattac gattccctca aggaagatat cgaggggatc gtcaagttca
       61 gttacatcca gagggtggag gtcgcggagg agggagggcg gtctttcttt tccggtgtcg
      121 aggttgatcc ttctttcgaa gtcagatcga tggatctcgt atatgattac cggggatccc
      181 tgagaaagat cggcgacgtc agcctgttca tcgatatcgg gcagatgcac acagaccttg
      241 gaaagaacgt cctgatattg ctttacatac aactttttgc aaccctggtc atttcgttga
      301 tcatcggggg gatctttcac ttcatgatag gtcgtcatct gagggggctg gccgacttca
      361 tgggaaaaga tgatccttcg accgggacat cttcctttat gcttgatgga aatttcagca
      421 ggaagaatga actgggtatg cttgtggacc acttcaatgg catgcgcgaa aggatccgta
      481 attacgtcgg ggagataagg gaatggcggg acctgatgca ctacgtgatc cagtatgatc
      541 ccagcgcgat catggttctc gacagggata tgaggttcat cttcgtcagc gacaaactgc
      601 tggagaacaa cggccttacc ctcgatgagg tcatcggaaa aagtttcagc gaggtattgc
      661 cccatcttga gggcaagtgg accgatatcc tcagccaggc gctgcagggc gagatactgg
      721 gatctgcgga tgatgtgatc ctccgcaagg atggaaggat cgacaacctc agatggtact
      781 gcaggccctg gttcaaggca gatgggcaga tagagggcac ggtttactac tcggaggtta
      841 tttccgagcg caaaaagatg gaaagggcgc tcttcgtgga gaaggagctt ttccggacga
      901 cactcttttc ggttggggac ggtttcatat ccactgataa cgacggaaag gtcctgctga
      961 tgaaccgcgt tgcccgggaa cttaccgggt gggatccgga tgaggccagg ggaaaggcct
     1021 tttcggaggt attgaacatc gtaaacgaaa aaacccgtga gaaagcccca gacccggtga
     1081 aagaggtcct ctatacaggg gagacctttg agatggccca gaattgcctt cttgtatcga
     1141 aatcgggcaa agaaatacca atagaggaca gcgctgcccc ggtcagggac attgatggag
     1201 atattaccgg cgtggtagtg gtgttccgtg actgtactga taaaagaaag agacaacagg
     1261 agatagagta cctgagttat cacgaccagc tgacaggcct ctataacagg cgttacttcg
     1321 aggaggaggt caatcgcctc gatgtgccga ggaacctgcc ccttgccatg gccatgctgg
     1381 atgtcaatgg cctgaaacta ttcaatgacg ccttcggcca cctcatggga gacaggctgc
     1441 tcaaaagggt cgcggaggtt ctgaagaacg agtgcagggc cgatgacatc atagccagga
     1501 cgggagggga cgaattcgtt gtcctcctgc cgaaaactgc ttcagatgac gctgtagcca
     1561 taatggatcg agtatcagaa gtaataaagg gggaaaaaat cgaagatatc cctctctcca
     1621 tatcctgggg agtagacgtc aagaccgatc ggggcgagag catagagaag gtctttgaaa
     1681 aggcagagga cagcatgtac cgcaacaaga tctccgaaaa gaccagttac catcacaggt
     1741 cggtccagtt gatcctggag accctctttg cccgatcgga attcgagaaa tcccattcgg
     1801 aaaatgtggg agccttgtcc gaaaggatcg gcaaagccat ggggctgagc cgtgtagagg
     1861 cgggggaact ccggacagcg ggggtaatcc atgacatagg caagatagcc acacccgggg
     1921 gagttctggg aaaagcatcg gcgctggagg aagaggaatg ggacgagatc aggaaacatc
     1981 ccgagaccgg tttcagtatt ctgagcaccg tcagcgagta tggtccccta gcggaggttg
     2041 ttttttctca tcacgaaaga tgggatggta caggctaccc ccgggggctc aggggcgaga
     2101 acattccact tttctcgagg atcatcgcgg tggcagaggc atgggatgca atgatcgccg
     2161 gaaggccgtg gaaaagaagt ctttctgaaa aggaagccat tacagaactg gagagttgcg
     2221 cgggtacgca gtttgatccc gatgttgtga aggtgttttt cgaaatcatc tcttcccccg
     2281 aaagcgggga agtgccggat atcgcgaacg gggattgaca cgggggttcc ccttcggcct
     2341 ggaggaatag agatgtcgaa aaagaatgaa cctggaaagg gcagagacaa ggtcgtcctg
     2401 ttcgaggatt ttttcacatc catgctcaat tctgttaccg aatccgcttt cctgataacc
     2461 caggatggca tagttcttgc ggccaatgaa acagtggcca aaagactggg cttttccagc
     2521 gggggggatc ttgtcggcag gaatatgtac gacctgctgc ccccggaggt ggcggaaagc
     2581 cgaaggagca aggttcagga agtggttctg acaggcgaac ccgtacagtt cgaggacgtt
     2641 cggtccggtc gctggataca caactccatc taccccgtct acgactctac gggtggggtc
     2701 agcaggcttt ccgttttcgc gatcgacata accagacaga aggcccagga ggagaggctc
     2761 tcgctgcttg gggagatggt ggacagcgca cctgcctcca taaccatcca tgacaccggg
     2821 gggaacttca tttacgtaaa cagggagacc ctgaggctcc acggttacac cgaagaggag
     2881 ttcatgtccc tgaacctcaa ggatctggac gttcccgaga gcgtggaact tctggaggaa
     2941 aggttccgca tgatagccga gaagggcgag atgtccttcg agtcggccca ttttcacaag
     3001 gatggctcgg tcatccccgt gcttgttatg gcaaagaacc tcgactggaa gggaacaccc
     3061 gccgtaatga gcatagccac ggacatccgg gagagcaaaa aggccgagca ggctcttcgc
     3121 gaaaacgagg aacttatcat ggcggtaatg gacaaccttc ctctgggtgt agcagtaaac
     3181 tccctggacc ccgctgtgga attcagttac atgaacgaca ggttctggaa aacctacaag
     3241 acttcccggg aggagttgtc cggccccgac gctttctgga gcgctgtcta taaagatccg
     3301 gaattcaggg aaaaaatccg ggaaagggtg ctgaaagact gcgaaagtgg tgaccccggg
     3361 aagatgcaat gggacgatgt gcctgtcaca agggaagggg aaaccttttt cgtttctgcc
     3421 cgcaataccc ctgttccggg atcttccctg atggtatccg ctgtctggga tgttacggaa
     3481 tacaaaaaaa cagaggacca gttgagaagg gcttcggaga ggcttcaggc gatctacgac
     3541 catagcccgc tactgatcag cgagttcgac ctggaaggaa gatacctcct tgtcaacagg
     3601 gcgatctcgg aattgttcgg tctttctccc cgggaactgg aaggaagaac tttttctgaa
     3661 cttctcccgg aagaggtctc ggaaattttc atgagacgta cccggcatgt gtgggattca
     3721 caaaaaccat tagcgacaga ggatacagtc gacatcggcg gggttaaaag gcatttcctt
     3781 acaatgcttt tcccgcttta cgaaaaggat ggacacataa actccatcgg ctccattgcc
     3841 catgacatta ccgaccgcaa ggaggcggat gactcgctga gggacgccct ggggcggctt
     3901 cgaagggtga ccggttccgt tatccaggta attgtcatgg ccgtcgaatc tcgagatccc
     3961 tacacggccg ggcaccagag gcgagtcggc gatctggcca gagccatagc gacggagatg
     4021 gggctgacgg accacgatgt ggaaggtatc cgcatagccg gggtgatcca cgatctgggc
     4081 aagatctccg ttccgtcgga gatcctcagc aaacccagga tactgaatga taccgagttc
     4141 gccattgtga aggagcatcc ccaaaagggc tacgatattc tcaaagatgt agattttgac
     4201 tggcccgtgg ccgagatggt cctccagcat catgagcgtc tggatggttc gggttacccc
     4261 aggggcctta aagatggtga gatacttctc caggcccg
//
LOCUS       DAOTIQ010000102         4189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_25140_length_4189_cov_4.509918, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000102 DAOTIQ010000000
VERSION     DAOTIQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25140_length_4189_cov_4.509918"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1581
                     /locus_tag="P5541_09015"
     CDS             346..1581
                     /locus_tag="P5541_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRV72164.1"
                     /translation="MTEKQYKTTHPWINFSLDLRRASHRLWMLLGEAQSKCRHISGVP
                     LRPEEARKLHQIYLTKGVRATTAIEGNTLSEQEVQAIIERNEKVSPSREYLKQEVVNI
                     VEACNSIARKIGSNGPEDDDIAPEEVMAYNGQVLKALPGTDDGSSPGKIRTFPVVAGS
                     YRGAPAEDCGYLLERFCEWMNDPAAFDLGERNRMASGILKSILAHVYLVWIHPFQDGN
                     GRTARLLEFRFLLEAGAPSPAGQLLSNHYNETREEYYRYLELSWRKQDGVLDFLEYAL
                     QGFVDQLEEQIVMIRLDQIEVAWENYIHTQFSDSSTEKRRKNLLLTISKETGTVPKNR
                     ILALDTDLAKFYMERTLKTFSRDLGDLEKKGLLKSDKDTVSANKERIIAFLPILKPEI
                     LPELLDQSARKRKTGNLKG"
     gene            1682..4063
                     /locus_tag="P5541_09020"
     CDS             1682..4063
                     /locus_tag="P5541_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRV72165.1"
                     /translation="MKKRPDLPSTELDVLKKENARLKALLANHGIPWECGIPKETVQK
                     TYPQGSKELTSHEKVRLFRSLFRGRSDVFAVRWESSAGRSGYSPACGNEWKRGLCGKP
                     ATKCSDCKNRDLLPLTDQVIFDHLKGKQTIGVYPLVEGDRCFFLAADFDGDEWEEDSL
                     AFITSCRQHDIPAVMEISRSGNGAHVWIFFSEPVQAVDARYLGAALVSYTCLEKRQLS
                     LSSYDRFFPNQDTLPKGGFGNLIALPLQKHPRDLKRTLFVDDSLTPYPDQWSFLGSVK
                     TMTGEELRDAIIRVSGGKHPLDVGMVDEEEQTPWVRVSSPTEKITGDLPGSMDLVLGN
                     QIYFPKKQLTQPLTNRLIRLASFQNPEFHKAQAMRLSVWTKPRIITCAENHQDHVSIP
                     RGCLEKVLDLLGKNSIQSVIHDERGTGSEISVQFKGEIRKDQKQAVDKILEHDFGVLC
                     APTAFGKIISAAAVIAHRKVSTLVLVHRKELQRQWKEQLENFLKINGGTVGQIGGGKN
                     RPGGLVDIAVMQSLSRMEDLPVFLDVYGQIIIDECHHISAFSFESILKQAGSKYILGL
                     TATPQRRDGHQPIIFMQCGPLRHSAKRSANAPARLEVWPRILPGADIPGGTEIQEIFR
                     TITNDRERNRRIADDALEAYREGRKILLLTERTKHIDNLKEIIGENIENCHILHGRLP
                     GKTREEILGKLAEMDGNTPRIIIATGRLIGEGFDHPPLDTLVLAMPISWKGTLQQYAG
                     RLHREHADKDDVRIYDYIETDNPVLAKMWEKRLKGYRAMGYSIVERPDPRLSS"
BASE COUNT         1174 a         1034 c         1061 g          920 t
ORIGIN      
        1 ctgagcggag cgaagcggaa cccgccagca ggcggacgtt ccgcgccctt tgcctgctgg
       61 cgggaatccg ggttggtttt taaggttttc ctgaatcctg tatccagtct cctactcctt
      121 cgcttttact gtgtctaact tctcacttct tacgctcttg atccattcga ttcacgattc
      181 ccggattttt ctattgactg gttgcccttg ctgtcccatt acccttttgg tttctggaca
      241 aaatagtata tgatacatat caggaaaagg tccatttaac tggaaaatat tcgtcaaata
      301 tcaactccag ttgtcaaaaa acaacagaaa agaaggacat tatcgatgac agaaaaacaa
      361 tataaaacga cccacccatg gatcaatttc agcctggatt tgcgccgggc aagtcacaga
      421 ttatggatgc tcctgggaga agcccagtcc aaatgcaggc atatatccgg ggttcccctt
      481 cgcccagagg aagccagaaa actgcatcaa atctacctta ccaagggagt aagagctact
      541 accgcgatag agggcaacac tctttccgag caggaggttc aggcgataat cgaaagaaac
      601 gaaaaagttt cgccttcaag ggaatacctt aagcaggaag ttgtaaacat agtagaagcc
      661 tgcaattcca ttgcccggaa aatcggaagc aacggacctg aagatgatga tatagctcct
      721 gaagaagtga tggcctacaa cggtcaagtg cttaaggctc taccagggac tgatgatggt
      781 tcttcgcctg gcaagataag gacctttcct gttgttgctg gttcttacag aggggctccc
      841 gcagaggatt gcggatacct tcttgagcga ttctgtgaat ggatgaacga cccggctgcc
      901 tttgatcttg gcgagcgcaa caggatggct tcaggaatat taaaatcgat acttgctcat
      961 gtttacctcg tctggataca ccctttccag gacgggaacg gcagaacagc caggcttctg
     1021 gagttcagat tcctcctgga ggccggtgct ccctctccag cgggacaact tctgagtaat
     1081 cactataacg agacaaggga agagtattat cgttatctgg aactttcctg gcgaaaacag
     1141 gacggtgtac ttgattttct ggagtatgcg ctccagggat ttgtggacca acttgaagaa
     1201 cagatcgtga tgataaggct cgaccaaatt gaagtggcct gggagaacta tattcatact
     1261 cagttcagcg actcatcgac agaaaagcga agaaagaacc ttctgctaac catatcgaag
     1321 gaaacaggca cagttcccaa aaacaggata cttgctcttg atactgatct ggccaaattt
     1381 tatatggaaa ggaccctgaa aactttttcc cgtgatcttg gagaccttga aaaaaaaggt
     1441 cttctaaaat cggataagga cactgtgtct gcaaataaag aaaggatcat cgcgtttctc
     1501 ccgatcttga aacccgaaat tttacccgaa ctccttgacc agagtgctag aaaaaggaaa
     1561 acggggaatc tgaagggatg acgaaattgt ccaaagcagg cgatacagga aaaagaccag
     1621 cctgggcaag ttgcaagaga gtgccagcct tccatagacg acctgaacgc gatcaggatc
     1681 gatgaagaaa agacctgatc tcccttccac cgaactggat gttctcaaga aagagaatgc
     1741 ccgactgaaa gcactcctgg caaatcacgg aatcccctgg gagtgtggta tcccgaaaga
     1801 aaccgtgcaa aagacgtacc cccaagggtc aaaagaactt accagccacg aaaaggtccg
     1861 cctgtttcgc agtctcttca ggggaagaag cgatgtgttt gctgtccggt gggaatcttc
     1921 cgccgggagg tcgggatatt cgccagcctg cgggaacgaa tggaagcgcg gtctgtgcgg
     1981 aaaacctgcg acaaaatgct ccgattgtaa aaacagggac ctcctgcctc tcacggatca
     2041 ggttatcttt gatcatctga aggggaaaca aaccattggg gtataccccc tggttgaagg
     2101 ggaccgatgt ttcttcctgg ccgccgattt tgacggtgac gagtgggaag aagattccct
     2161 tgccttcatt acatcctgcc ggcaacatga catcccggct gtaatggaga tatcaaggtc
     2221 agggaacggg gcccatgtat ggatcttttt ttcggagcct gtacaggctg tcgatgctcg
     2281 ctatcttgga gcagcccttg tcagttatac atgcttggaa aagaggcaac tatccctttc
     2341 cagttacgat cgtttcttcc cgaaccagga tactctcccc aaaggaggtt tcggcaacct
     2401 tattgccctc cctcttcaaa aacacccgcg agaccttaaa aggaccttgt tcgtcgatga
     2461 ctccctcaca ccttaccctg accagtggag tttcctgggt tctgtaaaga ccatgactgg
     2521 cgaagaactg cgggatgcca tcatcagggt cagcgggggt aaacaccctc tggacgttgg
     2581 catggtagac gaagaggagc aaacgccctg ggtgagagtg tcaagcccta ctgaaaaaat
     2641 caccggagac ttgcccggat ccatggatct cgttctgggg aaccagatct acttccccaa
     2701 aaaacaactt actcagccgc tgacaaaccg tctgatccgc ctggcttcct tccagaaccc
     2761 ggagtttcac aaggcccagg caatgagact atctgtgtgg accaagccaa gaataataac
     2821 ttgcgcagaa aatcatcagg atcacgtatc gatccctcga ggttgccttg aaaaagtgct
     2881 ggatctattg gggaaaaaca gcatccagtc tgttattcat gacgaacggg gaaccggaag
     2941 cgaaatctct gtccaattca aaggagaaat cagaaaagac cagaaacagg cggtagataa
     3001 aatactggaa catgatttcg gtgtgctctg tgccccgaca gcctttggca aaatcatttc
     3061 agccgctgcc gtcattgccc accgcaaggt gagcactctt gtccttgtcc accggaagga
     3121 attgcaaagg caatggaaag agcaactcga aaatttcctg aaaattaacg gaggtacagt
     3181 gggtcagatc ggagggggca aaaacaggcc cggagggttg gtggatattg ctgttatgca
     3241 aagcctgtcc agaatggagg acctcccggt gtttcttgat gtctacggac agatcattat
     3301 cgatgaatgc catcacatct ctgctttctc cttcgaatcg atactgaagc aggcagggtc
     3361 gaaatacatc cttggtctga ctgcgacacc tcagcgtcgt gacggccacc agccgatcat
     3421 attcatgcag tgtgggccac ttcgccacag tgccaaaaga tctgcgaacg ctccagccag
     3481 gcttgaggtc tggccccgca ttcttccggg agctgatatc ccgggtggca cggagatcca
     3541 ggagattttc aggacgataa ccaatgaccg ggaaagaaac cggcgaattg cagatgatgc
     3601 cctggaggca taccgtgagg ggcggaaaat ccttctgctg acggaacgaa caaaacacat
     3661 cgacaacctg aaagaaataa ttggagaaaa tattgaaaac tgccatatac tccacgggcg
     3721 actgcccgga aaaacaaggg aagagatact cgggaaactg gccgaaatgg atggaaatac
     3781 tccgagaata ataattgcca cagggagact tataggggag gggttcgacc atcctcccct
     3841 ggacaccctt gtcctggcaa tgccaatatc atggaagggc actctgcaac agtatgccgg
     3901 gcgcctgcac agggaacacg ccgacaaaga tgatgtccga atatacgact acattgaaac
     3961 agacaaccct gtcctggcaa aaatgtggga aaagcgcctg aagggttacc gagccatggg
     4021 gtacagcatc gtcgaacgcc ctgacccccg tctgtcatcc tgagcgcggc aaagcagtat
     4081 ccgccagctg gcggatgagg atctgacctt gggcccttct caccacagtg gacgcagagg
     4141 gtggggccac acagagaaaa ccattctttc tgagaaccgg ggacgcaaa
//
LOCUS       DAOTIQ010000103         4140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_25526_length_4140_cov_5.882007, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000103 DAOTIQ010000000
VERSION     DAOTIQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25526_length_4140_cov_5.882007"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..609
                     /locus_tag="P5541_09025"
     tRNA            513..609
                     /locus_tag="P5541_09025"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Sec,seq:tca)
     gene            662..1483
                     /locus_tag="P5541_09030"
     CDS             662..1483
                     /locus_tag="P5541_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HRV72166.1"
                     /translation="MKKKEFKLKGFSYPLTPLGRSSIVGPYPWHYGTEYLNILYRTDP
                     EVIASYLPYPLEPGEEPDLAYVAFSRWWSLWEEDSDMPWLNPERTQYLEAAIWTGCSF
                     EGRQGQICLHIWVDNDFSMARGWFMGFPKKLGQVFMTDYHPLNPGMARPGPGTRLKGI
                     ASAHGERLIEGEMEIVDSASPGELPRPMGLPLFHIRHFPSIEKGAPPSILELVSLGAE
                     NYRYGEIAWRGRGKLTFFPSEIEEHFALAPVEILGAFSFSSGYTFDGGRVLYSWV"
     gene            1594..2676
                     /locus_tag="P5541_09035"
     CDS             1594..2676
                     /locus_tag="P5541_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV72167.1"
                     /translation="MKKFALGLVCLMMVIGVSVAFAFQPVAEKDLKIGFVYVGPVGDG
                     GWTYMHELGRVDMEKAFPGVESFFVESVPEGPDSVRVMETFIRNGARLVFATSFGYMD
                     FVQEVAAKNPEVIFMHCSGYKTADNVGNYFGRMYQARYLSGLVAGKMSKKNVIGFVAA
                     NPIPEVIRGINAFTLGARKSNPDVKIKVVWLFSWFDPAREKEATLALIDAGADVIAMH
                     ADSGAAPQTAEEAGVYVVGYNNDMSHYAPTMHLTAPIWNWGMVYKHVVGQVTEGTWKS
                     SDIWWGLKEEFVGLAPISDAVPTDIVALVEAEKAKIIDGSWDVFHGPVKNQKGEVVVK
                     SGAKMTDGEMLGMNWFVEGVEGDIPK"
     gene            2759..>4140
                     /locus_tag="P5541_09040"
     CDS             2759..>4140
                     /locus_tag="P5541_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV72168.1"
                     /translation="MEVLEVVSAGRVPLVSMRGIEKTFVGVKANSGVDMDLERGEVHT
                     LLGENGAGKSTLMNILSGLYTADSGAIVINGEPRILRSPRDAIRAGIGMVHQHFMLVP
                     TLSVWENVSLGLDDQPFFLDKGRVIEKVGDIQKRYGINVDPMAPIWQLSIGEQQRVAI
                     IKTLYRSADILILDEPTSVLTPQETRALFRTIRQIKAEGHGVIFISHKLDEVMDISDR
                     ITVLRKGRKVASVNVKDVTKESLAEMMVGRKVVFQIGKTLQNPGRVVLEARELVVRGD
                     RGRVRVDHFSLDVRQREILGLAGVSGNGQGELCDALAGIRKTEKGSIKVDGCELAGRR
                     TREFQKRGVSYIPADRIGTGLVTGMNLRENIALRKYWRKEFTRFRLFIHWENIAGRTG
                     QLVERYSVVNPGLGFPARMLSGGNLQKLMLARELSDDPRVVIAVQPTWGLDVSATLFV
                     RETLLEQRDRG"
BASE COUNT          996 a          989 c         1220 g          935 t
ORIGIN      
        1 ccctcagtag gatttgacgt caaaagacct tcattgatag cccattggaa aaagatcttt
       61 aaagaactga gcctgagatt gaaggtcact ggagattcgt atttccctaa ataactcagg
      121 atactctctc gaagcatctt attttcccaa atttgtgggg aatgaatagc agggtaggac
      181 cgaaaaaagt ggccgacctg gtatcgatag tcctctatgg tccttttgct gttgccttgt
      241 gcttttcgga ataataggaa cgaagcaaga acctcttccc atgattgaaa atatgttttt
      301 cgtagcgata ctattctgga catattcgct ccctccttat gttagcaatc aaaatcttta
      361 aattgtaaaa acgaaaatta gccccaacgg actcgaaaaa tcttgacact gaatgcacac
      421 gctgttaaac aatttaaaaa aagattgtta cccgaaagct cagtaatatc atggacattg
      481 agtcctttaa gacctaaaat attgtggctg tcggagacga gtgtgctctg gtgagtgccc
      541 cgggcttcaa acccggtggg ggtcggtacg gccggccccg gtgggttcga tccccacacg
      601 tctccgccat ttttttgttt ttcccccgtg tacagggaag atgaacaagg gaggagaaaa
      661 gatgaaaaag aaagaattca agctgaaggg gttttcctac ccgctcaccc ctttgggccg
      721 ttcctcgata gttggccctt atccgtggca ctacggaacg gagtacctga atatcctcta
      781 caggaccgac cccgaagtga tcgcgtccta cctcccatac ccccttgaac cgggagaaga
      841 acctgacctt gcctacgttg ctttcagccg gtggtggtcc ttgtgggaag aagacagcga
      901 catgccctgg cttaacccgg agaggaccca gtaccttgag gcggccatat ggacaggctg
      961 cagcttcgag gggaggcagg gacagatatg ccttcatata tgggtcgaca acgatttcag
     1021 catggccagg ggctggttca tgggattccc gaagaagctg gggcaggtct tcatgaccga
     1081 ttatcacccc ctgaaccccg gaatggcacg acctggacct gggacacggc tcaaggggat
     1141 agcatcggct cacggagaga ggctgatcga gggagagatg gagatagttg atagtgcttc
     1201 accgggggaa cttccccgac caatgggcct tccccttttc catataaggc acttcccctc
     1261 catcgagaag ggtgctcccc cctcgatcct ggagcttgtt tcgcttgggg ccgagaatta
     1321 ccgatacggg gagatcgcat ggcggggccg tggcaaactg accttcttcc cttcggagat
     1381 cgaggagcat ttcgctcttg cgcccgttga aatcctggga gccttctcct tcagttcggg
     1441 gtacactttt gacggtggaa gggtcctgta cagctgggta tgaatcctta tttcaaactt
     1501 ccccgaaaag ggggttgaaa tttattgtcc ttttcgtcaa aatgtaaagt gacatggcaa
     1561 tggaaactca aattgcaggg gggtctggtg ctaatgaaga agtttgctct tgggctggtc
     1621 tgtctgatga tggtgatcgg agtctcggta gcctttgctt tccagcctgt ggcggagaaa
     1681 gacctgaaga tcggttttgt ttatgtcgga cccgtcggtg acggcggatg gacctacatg
     1741 cacgagttgg gcagggtgga tatggagaag gcctttccgg gagtagagag cttcttcgtc
     1801 gaatccgtcc ccgagggtcc cgattccgtc agggttatgg agacttttat caggaacggc
     1861 gccaggctcg tttttgccac ctcctttgga tacatggatt tcgtacagga agtggctgca
     1921 aagaatcccg aagtgatctt catgcactgc tcggggtaca agactgcgga caatgtgggt
     1981 aactactttg ggaggatgta ccaggcaaga tacctctcag gtctcgttgc agggaagatg
     2041 tcaaagaaga acgtgatcgg ctttgttgcc gccaatccta tccccgaggt cataaggggg
     2101 atcaacgcct ttacgctggg tgcgcgcaag tccaaccccg atgtgaagat caaggttgtc
     2161 tggcttttct cctggttcga tcccgcaagg gagaaagagg ccacccttgc tctcatcgac
     2221 gccggagctg acgttatcgc catgcacgcc gattccgggg cagcaccaca gaccgcggaa
     2281 gaagcgggtg tctacgtagt aggctacaac aacgacatga gccactacgc cccgaccatg
     2341 cacctcacag caccgatctg gaactgggga atggtctaca aacatgttgt gggccaggtg
     2401 acagaaggaa cctggaagtc cagcgacatc tggtggggtc tgaaggaaga gtttgtaggt
     2461 ctggccccta tctctgatgc tgttccgacc gatatcgtgg cgctcgtcga ggccgagaag
     2521 gcgaagatca ttgacggcag ctgggatgta ttccatggcc ctgtcaagaa ccagaagggc
     2581 gaagtggtcg tcaagagtgg cgcaaagatg accgacggag agatgcttgg aatgaactgg
     2641 ttcgttgagg gtgtggaggg agacatcccg aaatagagat cttttttcag ggggcgcgag
     2701 tcacgcgccc tttttttttc tcttcatggt ttagaatctt tcttgtagaa gaaaaccctt
     2761 ggaggtactg gaagtggtat cggcaggccg ggtgcccctt gtatcgatgc gggggataga
     2821 aaagactttt gtcggcgtca aggccaactc tggtgtagac atggacctcg aaagagggga
     2881 ggtccatacc cttctgggcg agaacggcgc aggcaagagt accttgatga acatcctgag
     2941 cggtctctat acagccgatt cgggagccat tgtcatcaac ggtgagccga ggatccttag
     3001 gagccctcgt gacgccatca gggcgggtat aggcatggta caccagcact tcatgcttgt
     3061 tcccaccctg tcagtatggg agaacgtttc ccttggtctc gatgatcagc ccttcttcct
     3121 ggataagggg agggttatcg aaaaagttgg ggatatccag aagaggtacg gcatcaacgt
     3181 cgaccccatg gcgccaatat ggcagctctc cataggagag cagcagaggg tagcgatcat
     3241 caagaccctt taccgcagtg ccgacatact gatccttgac gagccgacct cggttctcac
     3301 cccccaggag acaagggccc ttttcaggac catccgtcag atcaaggccg agggacatgg
     3361 agtcatattc atatcccaca agcttgacga ggtcatggat atatcagacc ggatcaccgt
     3421 tcttcggaag ggtaggaagg tggcctctgt taatgtaaag gatgtgacca aggaatcact
     3481 tgccgagatg atggtgggga ggaaggtggt cttccagatc ggcaaaaccc ttcagaatcc
     3541 cggcagggtg gtgcttgagg caagggaact ggtcgtcagg ggagaccgcg ggagggtaag
     3601 ggtagaccat ttctcactcg atgtcaggca gagggagata ctgggacttg ccggggtttc
     3661 gggcaacgga cagggagagc tctgtgacgc actggcaggc atccgaaaga ccgaaaaggg
     3721 cagtatcaag gtggacgggt gcgaattggc gggaagacgg accagggagt tccagaagag
     3781 aggcgtaagc tacattcctg cagacaggat cgggaccggt cttgtcacgg ggatgaacct
     3841 ccgggagaac atagccctca ggaaatactg gcgaaaagag ttcacgaggt tccgtctttt
     3901 cattcactgg gagaatatcg ccggcaggac aggtcagctg gtggaaaggt acagcgttgt
     3961 aaatccagga ttgggatttc ccgcacgcat gctgtccggt ggcaatctgc agaaactgat
     4021 gctggccagg gagctctccg acgacccgag ggtggtcata gcagttcagc cgacctgggg
     4081 gctggatgta agcgcgacac tgttcgtaag ggagacgctc ctggaacaga gggacagggg
//
LOCUS       DAOTIQ010000104         4042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_26317_length_4042_cov_7.989215, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000104 DAOTIQ010000000
VERSION     DAOTIQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26317_length_4042_cov_7.989215"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..736
                     /locus_tag="P5541_09045"
     CDS             260..736
                     /locus_tag="P5541_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRV72169.1"
                     /translation="MLNHQLLDSIIEGGFSFELLKNERRIGSSEEGAAYWKIHIGQTA
                     PALILRADNRFLLLVISGAVARVDLKTLASSLGVASLRMATPQEIKGHFGLTPGDVPL
                     IGLVLPSLIDRRLLDYDFIYGGSGDPGYTLKISPQALPFLNKDHSLIDVPTLSERA"
     gene            834..1793
                     /locus_tag="P5541_09050"
     CDS             834..1793
                     /locus_tag="P5541_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRV72170.1"
                     /translation="MKQRIAVFAIAAFLVLSLLFAASAGAVTEKEGLIPVKGGQVWYR
                     AVGLEKDAVPLLVLHGGPGMTHFYLESLEALASERPVIFYDQLGCGNSITTEEETLSL
                     WTVERFVEEVQEVRDALGLEEVHILGQSWGSMLAVEYYTWKPEPRGIRSLILAGPFMS
                     TQKWIADAKKMLKTLPEDIQRIVEESEAKGDFDNPDYLAAMDLFYVNFLCRLDPWPDS
                     ILKAFDSMGMEVYNYMWGPSEFTATGTLRYHQVIDKLRDIRVPTLFTAGEFDEARPVT
                     AAYYWSLVPGAEIHIVNDASHTHHLEKPEEFNTIISWFMKRNE"
     gene            complement(1995..2405)
                     /locus_tag="P5541_09055"
     CDS             complement(1995..2405)
                     /locus_tag="P5541_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72171.1"
                     /translation="MKTGKIRLLIAAMISFVFSIVYNGLVHLVILGTANKRVESLRRV
                     DFSSKAWISLLATLAISFLLPMIYSRFVSEKSYKKGLFFGFCFGLLIAIMVDVNQYVL
                     YPLPFSLVASWALFGVVEFSLIGLIVGLIVKDKT"
     gene            2760..3965
                     /locus_tag="P5541_09060"
     CDS             2760..3965
                     /locus_tag="P5541_09060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV72172.1"
                     /translation="MDYFVELHLPTLFFVMAAVILILAGTHVMVFLAYPEMPGVKLCA
                     LGNFLFALPIFALFLLPSGSVPAFSVLFNTLMTSGFLLIYLGIREYTGNGVQRGRLLL
                     TLAVFSGTYALFLSSIPWAFRTVAGLFTLGVFSFMTAWEACDGFRRIRTARLVFAGIF
                     CLHGVFLLAFAGAGLDLALSGKGALGQNPGTLGIVIIEGTLAALAVNLSYILLITDLL
                     SSRLARKAETDYLTGVYNRGGIIQMGEAMGQEPLSIIIADLDLFKKLNDKYGHAAGDK
                     VLRNFADTVEKNLRPWDALGRFGGEEFLILLPRLGAERASRIAERLRWEIEKTEISWE
                     SQPITYTVSMGISSRGENETKDLNRLIAEADEALYTAKSLGRNRVVWFDSGQMTIVRA
                     SEPLPGREA"
BASE COUNT          971 a         1097 c         1046 g          928 t
ORIGIN      
        1 ttaagggtat tcagccactt gcgccttgat ataaactatt tgggcaacag cccgtacact
       61 tttgactttt ttattctttt cataaaggga aaatcctctc tcccttcagg taccctggct
      121 tgaatgagaa agcatgaccg gtatatccac gaagttgaaa aaacccccaa agattccctc
      181 caaaatctat aatttcagga agatacctcc tggtcttccc cacggggatc aattcagatc
      241 atgaaggagg gtggtttagt tgctgaacca tcaattgctc gattcgataa tagaaggcgg
      301 gttctcattc gaactgttga agaacgaacg caggataggt tcttccgaag agggggcagc
      361 ctattggaag atccatatag gccagaccgc ccctgccctg atcctgaggg ctgacaacag
      421 attccttctc ctggtgatct caggagccgt cgccagggtc gaccttaaaa ccctcgcgtc
      481 atccctgggg gttgcgtccc tcaggatggc cactcctcag gagatcaagg gacatttcgg
      541 cctcacgccg ggagatgtcc ccctgattgg tctggtcctt ccctctctga tcgaccgacg
      601 cctgctggac tacgacttca tctacggagg gtccggcgac cccggataca ccctgaagat
      661 ctcaccccag gccctgcctt tcctgaacaa ggatcacagt ttgatagatg ttcccaccct
      721 ttcggaaagg gcttgatgaa agtaaacatt gcggaaggat aaagcccttt caccgtttca
      781 attttatgcc ttatactgta ttggggtaac tctttaaagg aggtgcgagg aaaatgaaac
      841 agagaatcgc ggtcttcgcg atcgcggcgt tccttgttct aagcctctta tttgccgctt
      901 ccgctggagc agtgactgaa aaggaggggc tgatccccgt caagggagga caggtctggt
      961 accgtgctgt gggcctcgaa aaggatgccg tcccgcttct ggtcctgcac ggcggaccgg
     1021 gaatgaccca cttctacctc gaatccctcg aagccctggc ctcggaaagg cccgtgatat
     1081 tctacgacca gcttggatgc ggcaactcca tcactaccga ggaggaaacc ctctcactgt
     1141 ggacggtgga acgattcgtc gaagaggtcc aggaagtcag ggacgccctt ggccttgaag
     1201 aggtgcacat cctgggccag tcctggggtt cgatgctcgc cgtggaatac tacacatgga
     1261 agcccgagcc caggggcatc aggagcctca tccttgcagg gcctttcatg agcacccaga
     1321 aatggatcgc tgatgcaaag aaaatgctca agaccctccc cgaggatatc cagaggatcg
     1381 tggaggaatc cgaagcaaag ggggacttcg acaaccccga ctaccttgcg gcgatggacc
     1441 tgttctatgt caacttcctg tgcaggcttg acccctggcc ggacagcatc ctgaaggcct
     1501 tcgactccat gggcatggag gtctacaact acatgtgggg gcctagcgaa tttaccgcaa
     1561 cgggtaccct gaggtaccac caggtaatag acaagcttag ggacatcagg gtgcccaccc
     1621 tgttcaccgc cggcgaattc gacgaagcca gaccagtgac ggctgcctac tactggagtc
     1681 tggtgccggg cgccgagatc cacatcgtga atgatgcatc ccatactcac catctggaaa
     1741 aacccgaaga gttcaacacc ataatttcct ggttcatgaa gaggaacgag taggagaaat
     1801 attccaggaa caggctaatg ggtatctgac aggcaaaaag cccaggcctg gcctctcgga
     1861 tcagaggggt caggcctgat cggtctgcgg ggaatgtttt gaacaaatgc ttttccaaca
     1921 gttccggcac agcgggggaa agatgggggc aaattgcaag gctcctctgg ctttccccga
     1981 tgagcccctg aacctcatgt cttgtctttg acgatcaaac ccactatcaa accgataaga
     2041 ctgaattcaa cgacaccgaa caacgcccag cttgcaacca gcgagaatgg caatggatac
     2101 aggacatatt gattgacatc gaccatgatg gctattaaca gcccaaagca gaaaccgaag
     2161 aaaagccctt tcttgtaact cttttctgaa acaaagcgtg aatagatcat cggcaacagg
     2221 aaggagatcg ccagggtagc caggagagag atccatgctt ttgatgagaa gtccactcgt
     2281 ctgagtgact caactcgctt gttggccgta ccaaggataa ccagatgaac gaggccatta
     2341 taaacgatcg aaaaaacaaa agagatcatt gcggcaatca gcaacctgat ctttcctgtt
     2401 ttcatctggc gtactctcct ccagagacat aacaaaagtc agatcgatct gcacaggata
     2461 cgggggttcc tttgttcctg gcgcgaaaga tttatttccc tccagcggct tgtcgttcag
     2521 acttccctta ccgatctgct ctcaagcctc cagcaggaac agtttacgcc cgatccggat
     2581 accccgtaaa gtgtcctggt ctgcctgaga atatcctcgt tttaccgcca gggtccaacg
     2641 gggaacccgt ccggccccct atgacccgta attgcccccc ctcaaagtga gataatccgg
     2701 agaatccgcg ttctttgaaa caggcatcag catccgtgct ccggaggaag aaggtcttca
     2761 tggattattt cgttgaactg cacctgccaa cgcttttttt cgtaatggcg gcggtcatct
     2821 tgatcctggc aggaacccac gtcatggtct tcctggccta tccagagatg cccggtgtaa
     2881 aactgtgcgc tttgggtaat tttctttttg cgttgccgat tttcgccctt ttcttgctcc
     2941 cctccggatc tgtccccgcc ttttctgtcc tctttaacac actgatgaca tcaggcttcc
     3001 ttctgatcta ccttggcata cgggaatata caggaaatgg ggtacaaaga ggacgccttc
     3061 tccttacctt ggcagttttt tccggaactt atgccctttt cttgtcgtcc atcccctggg
     3121 ctttcagaac tgtggccggg ttgtttacac taggggtctt ctctttcatg acggcttggg
     3181 aagcatgcga cggattcagg aggatccgaa cagcgaggtt ggtctttgca ggcatttttt
     3241 gcctgcacgg agtctttctc ctcgccttcg caggcgcagg cctcgatctt gccctttccg
     3301 gaaagggcgc cctggggcag aacccgggaa ctctggggat cgtcatcatc gaaggtactc
     3361 ttgccgccct agcggtaaat ctttcctata tcttgcttat tacagacctt ctctcctccc
     3421 ggcttgcaag aaaagccgag acggactatc tgacaggcgt ctacaaccgc ggcgggatca
     3481 tccagatggg ggaggcaatg ggacaggaac ccctttcaat catcattgcc gacctggatc
     3541 ttttcaaaaa actcaacgac aagtacgggc acgccgcagg cgacaaggtc ctcagaaact
     3601 ttgctgacac ggttgagaaa aacctcaggc catgggatgc tcttggacgt ttcggagggg
     3661 aggaattcct gatccttctg ccccggctgg gagcagaaag agcctctcgc atcgctgaaa
     3721 ggctgcgttg ggaaattgaa aagaccgaaa tttcctggga gagtcagccc atcacctaca
     3781 ccgtgagcat ggggataagc tcccggggtg aaaacgaaac gaaggacctg aacaggctta
     3841 tcgctgaagc cgacgaggcg ctttataccg cgaagagcct gggaaggaac agggtggtct
     3901 ggttcgattc cgggcagatg accattgtca gggccagcga accgcttccc ggtcgagagg
     3961 cctgaagacc aagtggcggc tcgtctgcag tgataatcga cagagcgcct acggaagtgc
     4021 ctttatgatc tccagcacga ac
//
LOCUS       DAOTIQ010000105         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_26629_length_4005_cov_4.048354, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000105 DAOTIQ010000000
VERSION     DAOTIQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26629_length_4005_cov_4.048354"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..520)
                     /locus_tag="P5541_09065"
     CDS             complement(104..520)
                     /locus_tag="P5541_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72173.1"
                     /translation="MKKSVLFFLAFFITLSAGIVCGAEEAHTPPVGSAERKAILDTMR
                     ADILENFDLEVVFVVKWLKVQDGWAWAETHPQSRDGLNRYEPFLALLEKREGIWAIAE
                     IPPLEEDAPPVDDAYFRDLIERFPDLPCEVFPWEKK"
     gene            complement(587..1465)
                     /locus_tag="P5541_09070"
     CDS             complement(587..1465)
                     /locus_tag="P5541_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV72174.1"
                     /translation="MQFKGELESARIIAVLDDYAGYETSFLAQHGIALLLEACSGGVC
                     KRILMDAGQSALPILHNMKLLDIDPASIDAVIISHCHYDHTEGLVDTLKAIGKKHLPV
                     IAHPGMFRPNYIFNPFIRNIGVMKENGPEAIEEAGGMMVMVDEPFEIMPGVIATGVVP
                     RKRDFEKQGIGTYNIEDGKVVPDQIIDDLSLAVKVKGKGLAVISGCSHAGIVNIIDWS
                     KEIAEEPRVEVVIGGFHLIEASQERIEKTAQALKQLNVGRVITGHCTGLPALCEFSKV
                     LGDRFEHLHVGTTYQM"
     gene            complement(1487..2788)
                     /locus_tag="P5541_09075"
     CDS             complement(1487..2788)
                     /locus_tag="P5541_09075"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HRV72175.1"
                     /translation="MTKGKQDLLSFGAIAVISAALFSGHFGVGDVIFPPILGKGAGSS
                     WFMAAMGYGIINSLGVLVAYLAVSRQQKTLLEMSSRTLGRAFGVVFTTICMLIIGPVF
                     ILPRVSSATHEMAVALFFPNFPLFATLAIFFALNYWVAYNRAQVIDRLGKVLSPALIV
                     FMAVLIIKGVVAPIAAVPATGSENPLADGVLNGYNTMNALGAALFGGWILKELAIRGI
                     DDKGAQSKNLAYIGPIVAFGLLITSTGITYLGATASTVFPDAGIGVYTVMIAEGLLGV
                     VGKAIFALLLAFACFTTSVGLTSTAGDVFQEMSGGKLQYKTIVALSSLVGFGIGMVGL
                     GKIVGYTVPWLMLVYPAIIVLLVGNLFDFGKFKPALQAGMVTAIVLSLGDFLSGMGME
                     GNFFSTMTAKLPLGGQGLGWLVPAVVAAVVALAVSGMSKKK"
     gene            complement(2963..3514)
                     /gene="cobO"
                     /locus_tag="P5541_09080"
     CDS             complement(2963..3514)
                     /gene="cobO"
                     /locus_tag="P5541_09080"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV72176.1"
                     /translation="MTEGEKGEHIFDPGLVQVYTGDGKGKTTAALGLTLRIAGHGGKV
                     AFIQFMKGWDFYGEIAGLALLPGVRHERTGTPDYVYRGKERPVDIDEAKRGLELAEDF
                     LASGDYDLVVLDEINVAVDYGLVDVDAVTRAVENRPRHVEVLMTGRSAPKEFLDMADL
                     VTEMKEIRHPYNKGLASRRGSDF"
     gene            3629..>4005
                     /gene="truD"
                     /locus_tag="P5541_09085"
     CDS             3629..>4005
                     /gene="truD"
                     /locus_tag="P5541_09085"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRV72177.1"
                     /translation="MKIKVNPEDFRVEEILSAPLSQDESPHRVYRLRKRNWNTVDALA
                     EISRAGELPLKELRWCGRKDRAALTTQYFSTTSRKDLTFKSTNVSAEFAGFSDSELRP
                     SSISGNRFSIAIRDLAEGELTNLS"
BASE COUNT          932 a         1154 c         1022 g          897 t
ORIGIN      
        1 aggtcaccga cggcctcgaa gagggggatg ttatcgtccg cgacgccagc ctcgatcttc
       61 gggaggggca gcgggtcagg ggctcaggcg caaggtaacc ttgttatttc ttctcccagg
      121 ggaaaacctc gcacggaaga tcggggaacc tctcgataag gtccctgaag taagcatcgt
      181 ccacgggagg agcatcctcc tcaagagggg gtatctccgc tattgcccag atcccttccc
      241 tcttttccag cagggcaagg aagggttcat agcggttcaa tccgtcgcgg gactggggat
      301 gggtctctgc ccaggcccag ccatcctgca ccttgagcca tttcacgacg aagacgacct
      361 ccagatcaaa attttccagg atgtcggccc tcatcgtgtc caggatcgct ttcctttcag
      421 cgcttccaac gggaggagta tgggcttcct ctgccccgca gacaatacct gcggacagtg
      481 tgataaaaaa agccaggaaa aacagaactg acttcttcaa ctccttcgcc ccctttaaaa
      541 aggcgacgcc tttccggggc gtcgcctcaa aaaacaacct cgtttcctac atctggtaag
      601 tggtgcctac gtggagatgt tcgaaccgat cacccaaaac tttggaaaat tcacaaaggg
      661 caggcagtcc tgtgcagtga ccggtgatga cacgacccac attgagctgt ttcagggcct
      721 gcgcggtctt ttctatacgt tcctgcgagg cctctatgag atggaagcct ccaatgacaa
      781 cttcgactct cggctcttca gcgatttcct tcgaccagtc tatgatgttg actatcccgg
      841 cgtggctgca tcccgagatg accgcaaggc cttttccctt tacctttact gccagggaga
      901 ggtcgtcgat gatctggtca ggcacgactt ttccgtcctc gatgttatag gtgcctattc
      961 cctgtttttc gaaatccctc ttcctcggga ccactccggt ggctattacg cccggcatga
     1021 tctcgaaggg ttcgtcgacc atcaccatca ttcccccggc ttcttcgata gcttcagggc
     1081 cattttcctt catcacgcca atatttcgga tgaaggggtt gaaaatatag ttcggccgga
     1141 acatcccggg gtgagcaatg acgggaaggt gctttttgcc gatggcctta agggtgtcga
     1201 ccagcccctc cgtgtgatcg tagtgacagt gagaaattat gactgcgtcg atcgaggcgg
     1261 ggtcgatatc caaaagtttc atgttgtgga gtatcggcag cgccgactgc ccggcatcca
     1321 tgaggatcct tttgcatacc cccccggaac atgcttccag aagcagagct attccgtgct
     1381 gggccaggaa cgatgtctcg taccccgcat agtcatcgag aacggcaatg atcctcgcgg
     1441 attccaattc acccttgaac tgcaatatta tcaatcctcc ccgcggttat ttcttctttg
     1501 acatacccga cacggccagt gccaccacag cggcaacaac ggccgggaca agccaaccaa
     1561 gcccctggcc tcccagggga agttttgcgg tcattgtgct gaagaaattg ccctccatgc
     1621 ccattccgct gaggaaatcg ccaaggctga ggactatggc agtcaccata ccagcctgga
     1681 gggcgggctt gaacttgcca aagtcaaaga ggttgcccac cagcagcact atgatagccg
     1741 gataaacgag catcagccat ggtactgtgt agccgacgat cttgccgagc cctaccatac
     1801 cgattccgaa gccgacgagg ctggaaaggg cgacgatggt cttgtactgc agtttgccgc
     1861 cgctcatctc ctggaaaacg tcacctgccg tcgaggtaag tcctaccgag gttgtgaagc
     1921 aggcaaaggc cagaagaaga gcaaatatgg ccttgccgac gaccccgaga agcccctctg
     1981 caatcattac ggtgtagaca ccgatacccg catcgggaaa tacggtgctg gcagtggcgc
     2041 caagataggt gatacccgtt gatgtgatca ggagtccgaa agcaactatg ggacctatgt
     2101 atgccaggtt cttcgactgg gctcccttgt cgtctattcc cctgatcgcc agttccttga
     2161 ggatccatcc tccaaagaga gcagctccca gggcattcat ggtgttgtac ccgttaagca
     2221 ctccatccgc cagggggttc tccgaccccg tcgcgggaac tgcggcaatg ggagccacga
     2281 ctcccttgat gatcagcacc gccatgaaaa cgatcagtgc cggagagaga accttgccaa
     2341 gccggtcgat aacctgtgcc ctgttgtagg ccacccagta gttgagtgca aagaaaatgg
     2401 ccagggtcgc gaacagtgga aagttgggga agaaaagtgc gaccgccatc tcatgggttg
     2461 ccgatgagac acggggcaga ataaagaccg ggccgataat gagcatgcat attgtggtga
     2521 ataccacgcc aaaggccctc cccagggtgc gggaactcat ttcgagaagc gtcttctgct
     2581 gccttgatac cgcaaggtaa gccacaagca cgcccaggct gttgatgata ccgtagccca
     2641 tggccgccat gaaccatgaa gaaccggccc ctttacctag tatcggcggg aatattacgt
     2701 caccgactcc gaaatgcccc gagaagaggg ctgccgaaat taccgcgatg gcaccaaatg
     2761 acaataaatc ctgttttccc ttcgtcactg gactctacct cctgatgaat tttggctttt
     2821 gagtctgatt atcaatacca gatcgcagcc gttgagcaat tctagcaaat attaaatcgt
     2881 ttgggtagag ttatgaagaa attgaaacat ttcggatcgg agcgcttgtg aatatcacca
     2941 ggtcttctgt cgcataaaac ttctagaaat cggatccccg ccttgacgca aggcctttat
     3001 tatagggatg ccttatctct ttcatctccg tcacaaggtc ggccatatcc agaaattctt
     3061 tcggagccga gcgccccgtc atgaggacct ccacgtggcg gggacggttt tcaaccgccc
     3121 gcgtgacggc atcgacatcg acaagcccgt aatctacggc gacatttatc tcgtcgagaa
     3181 cgacaagatc ataatcaccc gatgccagga aatcctcagc cagctccaac cctctcttcg
     3241 cttcatcaat atctaccggc ctctcctttc cacggtagac atagtccggc gtcccggtcc
     3301 tctcgtggcg gactccaggc agcagggcca gtccggcgat ctcgccgtag aaatcccaac
     3361 ccttcataaa ctggatgaat gcaacctttc ccccgtggcc ggcaattcta agggtcaggc
     3421 cgagggccgc agtagtcttt cccttgccat ctcccgtgta aacctgtacg agtccgggat
     3481 cgaaaatatg ctctcctttt tccccttcgg tcatttacga ctcccccctt ctcatgttcc
     3541 tgttcaccgt tgaaggtctc ttctcccccc ggatcgaaat ttgggttatc cttgtctggc
     3601 ggcaaagagc cgcacaggag gcaatatttt gaagataaag gtcaatcccg aagatttccg
     3661 ggtagaagag atcctttcag caccactttc gcaagacgag tcaccccacc gagtataccg
     3721 cctccgcaaa aggaactgga acaccgtgga cgctctcgcc gagatctccc gggcaggaga
     3781 actccccctg aaggaactcc gctggtgcgg gaggaaggat cgcgccgccc ttacgaccca
     3841 atatttctcc acgacatcgc ggaaagatct tactttcaaa agcacgaatg tctcggcgga
     3901 atttgccggt ttttccgatt ccgaattgag gccgtcatca atatcgggaa acaggttcag
     3961 tatcgccatt agggacctgg cagaggggga attgacaaac ctctc
//
LOCUS       DAOTIQ010000106         3913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_27460_length_3913_cov_7.109383, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000106 DAOTIQ010000000
VERSION     DAOTIQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27460_length_3913_cov_7.109383"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="P5541_09090"
     CDS             <1..834
                     /locus_tag="P5541_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72178.1"
                     /translation="PSRVPIAETETTMKIEQDGDRVSVTFGAFAGVHSATLFAGSFGQ
                     NRIVATWWYQGYPHETKVITGQYSPDTDRISGKMLYPRAADKPGLVPGWVEVNFVATR
                     KVEAVLPQPIQPVKPVIPAVVPLLLKEDCISFNPATTEVKNISGRWKVVDGSHWILDF
                     GANKGEADKSLAIIKHYGMDSVCFVGRPDPSMTYWRVNGQAPSGPVSGEDAIGFNPDT
                     IEVREINGRWKIVDGSHWIMDFEGNEGEARTAFQVIKKYGFRYICFVGRPDPSMTYFR
                     K"
     gene            complement(962..1918)
                     /locus_tag="P5541_09095"
     CDS             complement(962..1918)
                     /locus_tag="P5541_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV72179.1"
                     /translation="MELWHIFIQIFPYAVAFTIPLLITSLGGLFSERSGVVNIGLEGL
                     MVMGFFAAAITVKGLENSMGTGAIPLGLAAAALGGALFSILHAFASINLNADQIISGT
                     AINMMASAITVYLARTFTGSGNIRIRSGIIRQDIPLLSDIPVIGKMLFSQSYWSTWLV
                     IFILAVSWFLLYKTSFGLRLRACGEHPSAAASAGINVYFMRYMGVMISGAFAGLGGGI
                     ILVTYSGEFNGTVAGLGFLSLAALIFGQWKPLGILGATLFFGLAATIANVSQVIPQLA
                     VIPPLLLKSFPYAVTLMALVFFSRSSRAPKAAGQPYYPGQGQ"
     gene            complement(1920..3011)
                     /locus_tag="P5541_09100"
     CDS             complement(1920..3011)
                     /locus_tag="P5541_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV72180.1"
                     /translation="MKKGQNVLARALAAVFLGLVAGAVLMAAIGSNPLEGYNYLIRGG
                     LMNIERIGNTLATATNLTLTGLSVAFAFKTGLFNIGAAGQMLVGGLCSTAVALKVAAP
                     RPLLLILMVLAGFAGGAIWGSLPGYLKARFNVHEVVATIMMNWIAYWVVYYSVPAWLK
                     GTFLETESALISEAASLKTSWMNTFFQGSYINLGLFVALGAVIVIHVILKKTTLGFEL
                     QAVGYNRDAAENAGIRVERNMVLSMMIAGGLAGLAGTTFYTGYASNMQIGVLPSQGFD
                     GIAVALLGATEPAGVFASALFFGMLHSGKGFMNAMTSIPPEISDTIIATIIYFTATSV
                     LVGRLWDLWKQRGRGKTGAADTETRKDGI"
     gene            complement(3008..>3913)
                     /locus_tag="P5541_09105"
     CDS             complement(3008..>3913)
                     /locus_tag="P5541_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV72181.1"
                     /translation="ADRCTVIRRGKYIGTVDVKETSREQMASMMVGREVSFKVEKKPA
                     KTGNVVLAVESLSVLDSRKFRALKNFSLEVRSGEIIGLAGVEGNGQAELVEAITGLRK
                     PVSGKIMLKGIEITPLTRRERIELGVAHIPADRQKHGLVLEFTIEENMIIKNYYRPPF
                     SRNGFLQFDNIRENGRRIVELFDVRAGRGTESLAGDLSGGNQQKAIVGRELDSDPDLL
                     IAVQPTRGLDVGAIEYIHHRLVEQRDRGKAVLLVSLELDEIFNLSDQIAVINSGEVVD
                     IVKTSETVEEEIGLMMAGIRKEKAL"
BASE COUNT          869 a         1115 c         1074 g          855 t
ORIGIN      
        1 ccctccaggg ttccgatcgc ggagacggaa accacaatga agatcgagca ggacggagac
       61 agggtgtcgg tcactttcgg ggcttttgcg ggagttcatt cggcaaccct cttcgcaggt
      121 tctttcgggc agaacaggat cgtggccacc tggtggtacc agggttaccc acacgagaca
      181 aaggtcatta ccgggcaata ctcgcctgat accgacagga tctccggcaa gatgctttat
      241 ccccgggcgg cagacaagcc gggactcgtt cccggttggg tcgaggtcaa ctttgtggcc
      301 accaggaagg tcgaggcggt ccttccccag ccgatacagc ccgtcaaacc cgtgatcccc
      361 gcggttgtac cgcttctcct gaaggaagac tgcatctcct tcaaccccgc gactacggag
      421 gtcaaaaaca taagcggaag gtggaaagtg gttgacggaa gccactggat cctggatttt
      481 ggcgccaaca agggcgaagc ggacaagtcc cttgcgataa tcaagcacta cggaatggac
      541 agcgtctgtt tcgtcggaag gccagacccc tcgatgacct actggagggt caacggccag
      601 gcaccgtcag gccccgtttc gggagaggat gccattggat tcaaccccga cacgattgag
      661 gtcagggaga tcaacgggcg gtggaaaata gttgacggca gccattggat aatggatttc
      721 gaaggaaacg agggagaggc caggactgct ttccaggtaa taaaaaaata cggtttccgc
      781 tacatctgct ttgtgggcag gcccgatcct tcgatgacat atttcagaaa ataacgatcc
      841 cttcagggaa ccacaaaaca ataacggggg gcagcggcga agttcttgcc gctgcccccc
      901 gtagaactga cttttgtgtc aaaaaaacag gggaccagca agacagggaa tccactggct
      961 tttactgtcc ctggccgggg tagtagggct gccctgctgc cttgggagcg cgagatgacc
     1021 tggagaagaa caccagggcc atgagagtga ccgcgtaggg aaaacttttc agcagcagcg
     1081 gcggtatcac cgccagttga ggtatgacct gggaaacgtt ggcaatcgtg gccgccaggc
     1141 cgaagaatag cgtggctccc aggatcccca gcggtttcca ctggccgaag atcagggcgg
     1201 caagggaaag gaatcccagc cccgccaccg tcccgttgaa ttcccccgag taggttacga
     1261 ggattattcc tccgccaaga cccgcgaacg ctccggatat catgaccccc atgtaccgca
     1321 tgaaatagac gttgatgccc gccgatgcag ccgccgatgg gtgttcgccg catgctctca
     1381 gccggagacc gaaagaggtc ttgtaaagca gaaaccacga cacggcaagg atgaagataa
     1441 ccagccatgt gctccagtag gactgggaga agagcatctt cccgatcacc ggtatatccg
     1501 aaagcaacgg aatatcctgc ctgatgatgc cgctccggat gcgtatgttg ccgctgccgg
     1561 taaaggtccg ggcgaggtat accgttattg ccgaggccat catatttatc gccgttccgc
     1621 tgattatctg gtccgcgttg aggttgatcg aggcgaaggc gtggaggatg gaaaaaagcg
     1681 ctccgcccag ggctgccgct gcgagcccca gcgggatagc ccccgttccc atgctgtttt
     1741 ccaggccttt gacggttatg gcggcggcga agaagcccat gaccatgagt ccctccagcc
     1801 ctatgttgac aacgccgctt ctctcactga acagtccccc gagggaagtt ataagaagag
     1861 gtatggtaaa tgcgaccgca taggggaaaa tctggatgaa aatgtgccag agttccattt
     1921 cagatgccgt cctttcgcgt ttccgtgtcc gccgcgccag ttttgcctct gccgcgttgt
     1981 ttccagagat cccacagtct tcccacgaga acgcttgtcg ccgtgaaata gattatggtt
     2041 gcgataattg tgtcggatat ctccggagga atgctggtca tggcgttcat gaaacccttt
     2101 cccgagtgaa gcattccgaa gaaaagggcc gaggcgaaaa caccggcggg ttcggtcgcg
     2161 ccgagaaggg ccacggcaat accgtcgaat ccctgggacg ggaggactcc tatctgcatg
     2221 ttcgaggcgt atcctgtgta gaacgtggtt ccggcaagac ccgccagccc tcccgctatc
     2281 atcattgaca gtaccatgtt ccgttcaact cttatccccg cgttttccgc ggcgtccctg
     2341 ttgtagccta ccgcttgcag ttcgaacccc agggttgttt tcttcaggat cacgtggatc
     2401 acgatgacgg cccccagcgc gacgaataat cccaggttga tataagaccc ctggaaaaaa
     2461 gtgttcatcc atgaagtctt gagggatgcg gcctcggaga tgagggcgga ttctgtttca
     2521 aggaaagttc cctttagcca tgcggggaca gaatagtaga cgacccagta ggcgatccag
     2581 ttcatcatga tcgtcgctac gacttcgtgg acattgaacc gggccttgag atagcctgga
     2641 agggatcccc agatggctcc acccgcaaaa cctgccagga ccatgaggat gagaagcaag
     2701 ggtctcggtg ccgcaacctt gagggctact gccgtggagc atagtccacc gacaagcatc
     2761 tgtcccgccg cgcctatgtt gaacagcccg gtcttgaagg caaaagccac cgaaagaccc
     2821 gtcagcgtca ggttcgtggc cgtggccagg gtgttgccta tgcgctcgat attcatgagc
     2881 ccccccctga taagatagtt gtatccttcc agagggttgc tccctatggc ggccataagg
     2941 accgcgcccg caacaaggcc aagaaagacc gcggcgagtg ctcttgcgag gacgttctgt
     3001 ccctttttca tagggccttc tccttgcgta tgcctgccat catgagacct atctcttcct
     3061 cgacggtctc cgatgtcttc acgatatcga ccacctcgcc gctgttgata acggctatct
     3121 ggtcggagag gttgaatatc tcgtccagtt ccagtgacac cagcaggaca gcctttcccc
     3181 tgtctcgctg ttcaaccagt cgatgatgaa tatattcaat ggcgccgacg tccagacctc
     3241 ttgtaggctg gaccgctatc agcaggtcgg ggtcactgtc cagttcgcgt cccacaattg
     3301 ccttctgctg gttccctccc gacagatccc cggcaaggga ttctgtgcct ctccccgcgc
     3361 gtacgtcaaa aagttccact atcctcctgc cattttcccg gatattgtcg aattgcagaa
     3421 aaccgtttct ggaaaagggt ggcctgtagt agttcttgat gatcatgttc tcctcgatcg
     3481 taaattcgag caccaggccg tgcttctgcc tgtccgcggg gatgtgggca acacccagtt
     3541 ctatgcgttc ccgcctggtc aggggagtga tctcaatccc cttcagcatt atctttccgg
     3601 aaacaggttt tcgaagaccc gtgatggcct cgaccagttc tgcctggccg ttcccctcga
     3661 cccctgccag tccgatgatc tccccgcttc tgacttcgag agagaaattt ttcaaggctc
     3721 tgaattttct cgagtcaaga accgagaggg actccaccgc cagtaccaca ttaccggttt
     3781 tggcgggctt tttctctacc ttgaaagaaa cttcccttcc caccatcatc gatgccattt
     3841 gttcccgact cgtttctttt acatccactg tccctatgta tttgccgcgg cgaataacgg
     3901 tacacctgtc tgc
//
LOCUS       DAOTIQ010000107         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_29746_length_3681_cov_8.411197, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000107 DAOTIQ010000000
VERSION     DAOTIQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29746_length_3681_cov_8.411197"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..901)
                     /locus_tag="P5541_09110"
     CDS             complement(365..901)
                     /locus_tag="P5541_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV72182.1"
                     /translation="MKRKAFTLVELLIVIVIIGILAGSLLLVMGSGKAKAEATKIVSD
                     MRVLKAAAVMYFYDNSDWPADGDLDALKPYLDREPDAGNICSYSVANDGTHVFIGATL
                     TNCDEKTKSSLASMAEETGLLDSAMPLASLPSGNRLALIRPVMSLFEARPAWAVVVIE
                     GSSYYSGGDEAFMHVADY"
     gene            1110..1991
                     /locus_tag="P5541_09115"
     CDS             1110..1991
                     /locus_tag="P5541_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV72183.1"
                     /translation="MIRGEGVPPGNTGAVPFGGKFSPGAENLASVLDAMSESVIFTDP
                     EGVIFAASRAAGEHLGLGNGESLLGKNLFDSLPGGNGTLRRIRVAQVLETGNPVQFEE
                     ILFGRRFVNAIFPVKAADGSPVGLALFAMETMKGENTDIHKSDTERFIRSVVKDLPVG
                     IAVFPPEPGEGAVYIARPETLDSAGIFWEVVAGDLYPGENSLPEHRTVPGKGVSLRFS
                     VAGQGMDPSGTPVCPAGFYSFPVYPADPETIMIDRLDREGKYLVVNRHVCKMFGPSAR
                     HCRASKGMSFVFSGPGK"
     assembly_gap    2228..2327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2375..>3681
                     /locus_tag="P5541_09120"
     CDS             2375..>3681
                     /locus_tag="P5541_09120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV72184.1"
                     /translation="MHGYEPGELQNITISSIEDRSEDDITKGGANLPGPGGISYDTPC
                     LRKDGSVFPAKVFCRPVEWKGQPAVLRVASEMTGQRMAEEAIFFEKERFRTTLLSIGD
                     GVISTDSCRRILLMNRVAEDITGWKNAEARGQVLEKVLNVVHEDTSEMCEMLHFGQES
                     SCQGKAVLLARDGRKVPVESSLAPVSDMAGGSTGLVVVFRDCSEKRERQRRIEYLSYH
                     DELTGLHNRRFFDEELRRLDLPRNLPLSLVVVDVNGLKLFNDAFGHLAGDDLLKRVSE
                     VLKGQCRPEDVLARLGGDEFIMILPGSDPAVACQIASGIGNSMSMEEVEGVPLSVSCG
                     WGTKFDMDTGIRKVFEMAEDHMYRNKISQKTVHDQRSVRIILETLFSRMPGERAHSQN
                     VGRLCERIGHALGLCQSEIGELKTAGIIHDIGKIGISRFTIRK"
BASE COUNT          813 a          852 c         1033 g          883 t
ORIGIN      
        1 cattttccca ggagatacag gttcaagaga ttgcatcagc gaatcttatt acgaaatctg
       61 ttaatggcgc ccagggtatt tcttcccccg gtagactgcc tgaattgata aatcttccgg
      121 gaccgttctg tcaaagaaaa attcttgccc gtcggagtga ttttagaagg attttctcgc
      181 ctgaaggcca cggttttatt gccaatgcgg ggtttttcag tggtgaccgt tctaaaaccc
      241 cggtacactc aaaatccgca cccgctttgg cctgtggggg ttcgaatccc ccctccggca
      301 ccatagaaaa gacccagggg ctctgtcgct aacgtgtcag agcccctgtt ttattgggtt
      361 actctcagta atcggccacg tgcatgaagg cttcgtcgcc tccgctgtag taggatgatc
      421 cttcgatgac aacgaccgcc caggccggtc ttgcctcgaa aagactcatc actggcctta
      481 tgagtgctaa ccggttaccc gatggtagcg aggccagggg cattgcgcta tcgagcagtc
      541 ccgtctcctc ggccatggag gccagcgagg atttggtctt ttcgtcgcag tttgtcaagg
      601 tcgctccaat aaagacgtga gtgccatcgt tggctacgga ataactgcag atgttgcccg
      661 catcgggttc gcggtcgagg tagggtttca gggcatccag atcgccgtcg gcgggccagt
      721 cgctgttgtc gtaaaagtac atgaccgcgg cggccttaag caccctcatg tcgcttacga
      781 tcttcgttgc ctcggccttg gcctttcccg accccatgac cagcagcagc gatccggcga
      841 gtatcccgat tatgactatc acgataagca gttcgacaag agtgaaagcc tttcttttca
      901 tggaaaaatt tcctcctcat acttgcgtgg tttagatgaa agcagacggt agcaggagcg
      961 ggtaattcag cccgggaccg ttcatggcaa ctggtttcac ggtatgaaat atttgtgatt
     1021 ctggtattat tataaataat attccgtaaa gtataagaga tgcagtctcc actgacaaat
     1081 ttgttttttg ccatccatgg aggtgttcca tgattcgcgg tgagggtgtt ccacctggaa
     1141 acaccggggc cgttcctttc gggggaaaat tttcccccgg cgcggaaaac cttgcttcgg
     1201 tccttgatgc catgagcgaa tcggttattt tcaccgatcc tgaaggtgtg attttcgcgg
     1261 caagcagggc agccggggaa catctgggtc ttggaaacgg ggagagtctt ctcggcaaaa
     1321 atctttttga ctctcttccc ggggggaacg ggacactgag aaggatacgg gttgcccagg
     1381 ttctcgaaac ggggaaccca gtccagttcg aagagatcct tttcggcagg agattcgtca
     1441 acgccatatt ccccgtgaag gcagccgacg gatctccagt gggtctggca ctgttcgcta
     1501 tggagacaat gaagggggaa aatacagata tccataaatc ggatacggaa cgtttcataa
     1561 ggagtgtagt gaaagatctg ccagtgggca tagcggtttt ccctccggaa ccaggcgagg
     1621 gagcggttta tattgcccgg cccgagaccc tggattctgc gggaatcttt tgggaagtgg
     1681 ttgccggaga cctgtatccc ggggaaaact cgttgccgga acacaggacc gtgcccggga
     1741 aaggggtttc tttgaggttc tctgttgcag ggcagggaat ggatccttcc ggtaccccgg
     1801 tctgtcctgc cggtttttac tcttttccgg tatatcctgc tgacccggaa accatcatga
     1861 tagacaggct cgacagggaa ggcaaatacc ttgtcgtaaa cagacatgtc tgtaaaatgt
     1921 tcgggccttc ggcccgtcac tgccgtgcct caaaagggat gtcttttgtt ttttcgggcc
     1981 ccggcaaata acctttacgc ggggctttac gggcaagacg gtttcacgct atccccttat
     2041 ttcaggaaaa ccggaggatg tgttgctttt tgcctgtgac caggagaacc gcgggtgttt
     2101 ttcttgttgc tattgccacg gggttttgtc tatggcttga attttacttc catctcggaa
     2161 gggggtatac ggaggtttat ccctcttttt tcttcatacc tgtcatttcg gcatcgctgc
     2221 tcctgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg atgctggagg
     2341 gaggttcctt tactggaatc gtgagaccct tgatatgcac ggatatgaac ctggagagtt
     2401 gcaaaatatt accatttcga gcatagaaga tcgctccgaa gatgacatca caaaaggagg
     2461 agcgaacctg ccggggcccg gggggatttc ctatgatacg ccgtgtctcc ggaaggacgg
     2521 ttccgtgttc cctgcgaagg ttttctgcag gcctgtggag tggaaggggc agcctgcggt
     2581 ccttagagtg gcgagcgaga tgacgggcca gagaatggcg gaagaggcca ttttttttga
     2641 gaaggagcgt ttcaggacaa ctcttctctc gataggtgac ggagttatat caacagacag
     2701 ttgccgcagg atactgttga tgaacagggt cgcggaagat attacgggct ggaaaaacgc
     2761 cgaggccaga ggccaggtgc ttgaaaaagt cctgaacgtc gttcatgaag ataccagtga
     2821 aatgtgcgag atgctgcatt ttggccagga gtcgtcctgc cagggaaagg cggtgctcct
     2881 ggcaagggac gggagaaagg tccctgttga aagcagtctg gctccggtca gcgatatggc
     2941 tggcggctca accggcctgg tcgtggtatt cagggactgt tccgagaaaa gagaaagaca
     3001 gagaaggata gagtatctca gttaccatga cgaactgacg gggctccaca accggaggtt
     3061 tttcgacgag gaactgaggc ggcttgatct gcccagaaac cttcccctct ctcttgttgt
     3121 tgtcgatgtg aacggtctca agcttttcaa cgatgctttt ggccaccttg ccggtgacga
     3181 tcttctcaaa agggtttcgg aagtcctgaa gggacaatgt cgcccggaag atgtcctcgc
     3241 aaggctgggt ggggatgaat tcattatgat tctgcccggg tcggatcctg cggtggcctg
     3301 ccagatagcc tcaggtattg gtaattccat gtccatggaa gaggtcgaag gggttcccct
     3361 ttctgtctca tgcgggtggg ggacgaaatt cgacatggat accggcatca gaaaggtctt
     3421 cgagatggcc gaagatcaca tgtaccgaaa caagatatcc cagaagaccg tccatgacca
     3481 gagatcggtg agaataatcc ttgagaccct cttctccagg atgcctggag aaagggctca
     3541 ttcgcagaac gtgggacggt tgtgcgagag gatcggacat gccctcgggt tgtgccagtc
     3601 ggaaattgga gaactgaaaa cagcgggtat tatccacgac ataggcaaga taggcataag
     3661 ccggttcacc ataaggaaga a
//
LOCUS       DAOTIQ010000108         3560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_31068_length_3560_cov_9.085307, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000108 DAOTIQ010000000
VERSION     DAOTIQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31068_length_3560_cov_9.085307"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1768
                     /locus_tag="P5541_09125"
     CDS             464..1768
                     /locus_tag="P5541_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV72185.1"
                     /translation="MKRKIMQDLIRWKEKEKRKPLIIQGARQVGKTWILKHFGEEHFR
                     NIAYINFDNNPRMEEVFRGDFNINRLLDAFQIETGKTVDPATLLIFDEVQEVPKAIQS
                     LKYFNEERPDIPVAAAGSLLGIAHHSGISFPVGKVELLDMYPMSFTEFLQALGETDLM
                     LLLEKGDFEMIKVFRDRYTTLLGQYFYIGGMPEAVGIFAETGNYLEVRSYQKNLLRLY
                     EQDFSKHIVPNTAERARMVWRSIPSQLSKENSKFIYGHLRPGSRSKDYEDAIQWLVDC
                     GLVIRIPRISRPGIPLAAYQDISAFKLFIHDVGLLTAMGDVDIKILLEGDRIYREFKG
                     SLTEQYVLMQLVADLGFEPSFYSAEKSSGEIDFVIQHKGAVVPIEVKAAENLQGKSLK
                     AFSEKFGSKRAIRFSLANYREESWMTNIPLYAVAGLRMVLDK"
     gene            1836..2384
                     /locus_tag="P5541_09130"
     CDS             1836..2384
                     /locus_tag="P5541_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV72186.1"
                     /translation="MRVLVLDGNNTSCSPYREDLEGVLDSLAKAGHEVNILRLAEKTV
                     HGCTGCFSCWLKTPGECVFKDDGVDFLRRMVHSDLVLFVSPLVMGFPTALLKNAIDRF
                     IPLVLPFLETADGECRHPLRYDRIPLFAFLYEPEHDTDEEDVEIVSTVFERFARNAHT
                     KFLFAMPLSSDPVGVKHALENI"
     gene            2365..3012
                     /locus_tag="P5541_09135"
     CDS             2365..3012
                     /locus_tag="P5541_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV72187.1"
                     /translation="MRLRIFNCSPKGRSSNSRVISGAFTRGFCELPGNEAEEFLVARE
                     KDMEKGLASFGEAGVNLVVFPVYVDAMPGLAKQFIERLAPLKGRLSDTRLLFLVHTGF
                     PEETHTRPMERYLNKLAVRLGANLDGVIRLGGGKGIKSSDSHQKNKALDELTCLGRIY
                     AVDGKLDARIMGRLAGPSRIPMLIARVMFPIVNYFGFGRELRKNGAWERRFDRPL"
     assembly_gap    3093..3192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          867 a          804 c          962 g          827 t
ORIGIN      
        1 gttcgtgctg gagatcataa aggcacttcc gtaggcgctc tgtcgattat cactgggacc
       61 cttaaaggtt cagacctaca caattgatga caaaagggcc ttttttgaaa agtacaggct
      121 aatttgacct ggataatacc ggggcgatag ttgcccggat atgaagatct ccccagtggg
      181 agctggcggt tcagcggcgc gaagctgaaa gagatcgtga accttgtagg ggtcaacccc
      241 acccttttga ctatttggca gaaatgggag aaaagtgagc ttggcaagga tcgggacagg
      301 agtgttttct cctggccaga cagctggacc cgcactgagt ggcgcttttt gtcaccccgc
      361 cagccgctgg aatatgctcc ttacgcataa agtcggacgt aaaaatgttg tttatgttga
      421 aaagtaggac gaaaaggtgt aagttactcc cggaggcgac gacatgaaac ggaagatcat
      481 gcaggatctg atacggtgga aagaaaaaga aaaaaggaag cctcttatca ttcagggagc
      541 gaggcaggtt gggaaaacat ggatcctgaa gcatttcggc gaagagcatt tcagaaatat
      601 cgcctatata aactttgaca acaatccccg gatggaggaa gtattcaggg gagacttcaa
      661 catcaaccgc ctgctggatg ccttccagat cgaaaccgga aagactgtcg atcccgcgac
      721 cctgctgata ttcgacgaag tccaggaagt gccgaaagcc attcaatcgc tcaaatactt
      781 caatgaagaa aggccggata ttcctgtcgc ggctgccgga tcgctgttgg gaatagccca
      841 ccacagcggt atttcctttc cggtagggaa ggtggaactg ctggacatgt accccatgag
      901 ttttaccgaa ttcctgcaag cacttgggga aacggatctc atgttgctgc ttgaaaaggg
      961 cgattttgag atgataaaag tattcaggga cagatacaca acactgctcg ggcaatactt
     1021 ttacatcggc ggaatgcccg aagcggtagg aatatttgca gagacaggaa actaccttga
     1081 agtcaggtct taccagaaga acctgctgag actttacgaa caggatttct caaaacacat
     1141 agtcccaaac acggcagaaa gagcgcgcat ggtgtggagg agtatcccct cgcaactctc
     1201 gaaagaaaac agcaagttca tctatggcca cttgaggccc gggtcccgtt caaaagacta
     1261 cgaggacgcg atccagtggc tggtggactg cggtcttgtc atcaggatcc cgcgcatatc
     1321 aaggccgggc attcctctgg cggcctatca ggacatttcc gccttcaaac ttttcattca
     1381 tgatgtaggg ctattgactg ccatggggga tgtggacatc aagattcttc ttgaagggga
     1441 ccgaatctac agggaattca agggtagtct gacggagcag tacgtcctga tgcagttggt
     1501 ggccgatctc gggtttgaac cgtcctttta ctctgcggaa aagtcaagcg gagagatcga
     1561 ttttgttatc cagcacaagg gtgccgttgt tccgatagag gtgaaggcgg cagaaaacct
     1621 ccagggcaaa agcctgaaag ctttctccga aaaattcggg tccaaacggg cgatacggtt
     1681 ttccctggcg aattatcggg aggagtcctg gatgaccaac atcccgctct acgccgtggc
     1741 tggtttgaga atggtcctgg ataaatagaa acctcatagg tgtcaggcct gcaaaggctg
     1801 ttgtccaata atgtttttct gcggggtgag gaactatgag ggtgctcgtt ctggatggca
     1861 acaacacgtc ttgttcccca tatcgggaag atctggaggg tgttctggat tctctggcaa
     1921 aggctggtca cgaagtgaac atcctgaggc ttgcggagaa gacagttcat ggatgcacgg
     1981 gctgcttttc gtgctggctg aagactcccg gcgagtgtgt cttcaaagac gatggggttg
     2041 attttttgcg tcgcatggtt cactcggacc tggtgctctt tgtctccccg ctggtaatgg
     2101 gtttcccaac ggctctgttg aagaatgcca tcgacaggtt catcccgttg gttctgccct
     2161 tcctggaaac ggcagatgga gagtgccgtc atcccctcag gtacgacagg atccccttgt
     2221 ttgcctttct ttatgaaccc gagcatgaca ctgacgaaga ggacgtggag atagtctcca
     2281 cagtctttga aaggtttgcc cgtaacgccc atacgaaatt tctttttgcc atgccgcttt
     2341 ccagcgatcc ggtgggggta aaacatgcgc ttgagaatat ttaactgttc cccaaaggga
     2401 aggagcagca actccagggt tatttccgga gccttcactc gggggttctg cgaattgccg
     2461 ggtaacgagg cagaggaatt ccttgtggcg agagagaagg atatggaaaa gggattggca
     2521 tcttttggtg aagcgggagt aaatcttgtg gtttttcccg tctacgtgga tgccatgccg
     2581 ggtcttgcaa agcagtttat cgaaagattg gcacccctta agggacgcct ttccgacaca
     2641 agacttcttt ttctggtcca tacgggtttt cccgaggaga ctcatacccg cccgatggaa
     2701 aggtatctta acaaacttgc ggttcgcctg ggcgcgaatc tggacggtgt gataaggttg
     2761 gggggaggca agggaataaa gagttccgac tcacaccaaa agaataaagc tcttgatgaa
     2821 ttgacttgcc tgggccggat atacgccgta gatggcaaat tggatgcccg tattatgggg
     2881 agacttgcag gtccttcccg cattcctatg ttgatcgccc gggtgatgtt ccctatcgtg
     2941 aactattttg gtttcgggag ggagctgcgt aaaaacggcg catgggaaag gcgatttgac
     3001 aggcccctct gagtctggaa tcggaaaatc cgtgagacgt gagacgtgag acgtgagacg
     3061 tgagacgtga gacgtgagac gtgagacgtg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnacgtgctt tgtcattctg agcggagcga agaatctggt tttgcctttc
     3241 aggttcccca gccaccagac tcccgtctcc tcgccttttc gatccacgat tcacgactaa
     3301 cggggcctaa acaggctcct tcgatcgcgc tcctcaggat gacaaaaaac aaaggcgtac
     3361 ccggacccgc tcctactcta ctacaggtgc cctccacccc caaagatcct gtcggggcct
     3421 gttttttctg atcaggagag ccttaaaggt gtcacctaca caattgatga atattcttga
     3481 aaggtgaaga tgttgcaagc agcttatagg ggtcaaacct acacgggctg ttgcccaatt
     3541 gagctcccca ggttttaaaa
//
LOCUS       DAOTIQ010000109         3557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_31108_length_3557_cov_6.051685, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000109 DAOTIQ010000000
VERSION     DAOTIQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31108_length_3557_cov_6.051685"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="P5541_09140"
     CDS             complement(<1..458)
                     /locus_tag="P5541_09140"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRV72188.1"
                     /translation="MPRREKIAFIGTGGTISMTFNEKQDGFIPTLSAQDLLDLLPEDC
                     MDNIHVVDWSHQPSSHYTIRMTADLVELMKKLVKDGISGIVLSCGTDSLEEMAYLTDL
                     LWNYPQPVVFTGSMQPPCRQGSDAIANLTQSIRAASSESLWGMGVVACFQ"
     gene            556..1920
                     /gene="trkA"
                     /locus_tag="P5541_09145"
     CDS             556..1920
                     /gene="trkA"
                     /locus_tag="P5541_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRV72189.1"
                     /translation="MRILIIGAGEVGYSVASSLSGEGHDITMIENDEERAAKAENELD
                     VMVVRGNGSRPPVLERAGLCPGCDIDLLIACSNHDEVNIMACWIARRSGVKRVIARAR
                     GMEYTDSPTWARELGIDVMISPERSVSREIEESLSISSAVHTAEFFGGKVGVYAFNVR
                     EGSPLIGISLRELRRLYPRITALVVFIERKGKGFVPFGDNKVEAGDLCFTVTSKSQAH
                     DLEELFTRQKSPVPRRVIIVGGGKIGFQVSRRLETRYPRLDIRLIDRDRDKCEKLSRE
                     LERTVVLHGEGMDAELLRNEGVEGADGFVTTTSIDETNLVMGAVGKMLGARKCIAVVR
                     RELLMEIGGHISVDDMVNPNQALATVIMRQVRYPSTAGVLSIINKIEAEVLEVTVPQG
                     SPVSGRRIMDLELPRGILVALVSRRGEMFVPWGDTCLEEGDEILLFASKELMPAALEI
                     LGVK"
     gene            1922..3373
                     /locus_tag="P5541_09150"
     CDS             1922..3373
                     /locus_tag="P5541_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRV72190.1"
                     /translation="MRLAVVARVLSLLCAIISLFMFFPIIWSLTDGSSDLQPLAIGML
                     SGFSLSVLLFLCGINAKAKDLGVREAFAVVSLSWVLASAIGGVPYLLHGSVPTFTDAF
                     FEAMSGFTTTGASVLADIQSNPRGILFWRSLTHWLGGMGIIVLSLAILPFLGVGGMQL
                     YKAEVPGPIPEKITPRVQQTAILLWGVYVLLSALQTAALMLGGMNLFESLTHTFGTMA
                     TGGFSPLNGSIGQYGNAYFEWVITLFMFLAGVNFVLHLLVLRGNPAALCRDEEFRFYG
                     KIALAAILATTVSLFLAGTYENISDAFRYGTFQVISILTTTGYVTADYEKWPFFIQYL
                     LLFLMFVGGCAGSTGGGIKNMRIMVMSRHVGAELQRLLHPRATVPVRVGGRVVEREII
                     SSVTAFFVMYIGLFALAAMAMSALGLDVLTAIASVAATIGNIGPGLGTVGPTENYAHI
                     PAAGKWVLSFCMLLGRLEIYTVMMIFVPGTWRR"
BASE COUNT          776 a          882 c         1042 g          857 t
ORIGIN      
        1 tcctgaaagc aggcgaccac tcccattccc cacaggctct ccgaagaggc ggccctgatt
       61 gactgcgtca ggtttgcaat tgcgtccgat ccctgcctgc aggggggctg cattgagcca
      121 gtgaaaacga cgggctgggg atagttccac aacaggtccg taaggtaggc catctcttcc
      181 agggaatcgg tgccacaact gagcactatc ccggatatgc cgtccttgac gagttttttc
      241 attaattcca caaggtctgc ggtcatccgt atggtatagt gactgctggg ctggtggctc
      301 cagtcaacta catgtatatt gtccatgcag tcctccggga ggaggtccag aaggtcctgt
      361 gcagacaggg taggtatgaa gccgtcctgc ttttcgttga aggtcatact gattgttcca
      421 cctgttccaa taaatgcgat cttctcacgg cgcggcatac agccacctcc ctttgctcgc
      481 aaaagtatag cacttttgca aaaagagtca ggccgctgcc ctgagaagat aaaatgcctt
      541 caaggtggtg cttttttgag gatattgata attggcgccg gggaagtcgg ttacagtgtt
      601 gcttcaagtc tttcgggcga agggcacgac attaccatga tcgagaacga tgaagaaaga
      661 gctgccaaag ccgagaacga actcgacgtt atggtagtga ggggaaacgg gtcccggccc
      721 ccggtgcttg aaagggcagg tctatgccca ggctgtgaca tcgatcttct gatagcctgt
      781 tccaaccatg acgaggtcaa tattatggcc tgctggatag cgcggaggtc cggagttaaa
      841 agggttattg ccagggccag ggggatggag tataccgaca gtccaacctg ggcccgagaa
      901 cttggcattg atgtcatgat atctcccgaa agatctgtct ccagggagat cgaggaatcc
      961 ctttcgatca gctcggctgt ccacaccgcc gagttttttg ggggcaaggt cggagtctac
     1021 gctttcaacg tcagggaagg ctctcctctt atcgggatct cgttgagaga gcttcggagg
     1081 ttgtatcccc gaataacggc acttgtggtg ttcattgaaa gaaaaggcaa gggatttgtc
     1141 ccttttggag acaacaaggt cgaagccggt gacctgtgct ttaccgtaac atccaaatcc
     1201 caggcccatg atctggagga acttttcacc aggcaaaaga gccctgtacc caggagggta
     1261 attatagtcg gcggagggaa gatcgggttc caggtttcca ggaggctgga gaccaggtat
     1321 ccaagacttg atattcgcct catagaccgt gaccgcgaca agtgcgagaa actttcccgc
     1381 gaacttgaga gaactgtggt cctgcatggc gagggcatgg atgctgaact cctcagaaac
     1441 gagggtgtag agggggccga cggttttgtt accacgacat cgattgatga aaccaacctt
     1501 gtcatgggcg cggttggaaa aatgcttggt gcccggaagt gcatagccgt ggtcaggcgg
     1561 gaattgctca tggagatcgg cgggcatatt tctgttgatg atatggtcaa ccccaaccag
     1621 gcccttgcga cggtaataat gaggcaggtc cgttacccgt cgaccgcggg tgttctctcg
     1681 ataatcaaca agatagaagc agaggtgctc gaggtaacgg tgccgcaggg ttccccggtg
     1741 tcgggcagaa gaataatgga cctggaactc ccgaggggga tccttgtggc cctggtatcc
     1801 aggcggggag agatgttcgt gccctggggg gatacgtgcc tggaggaagg ggacgaaata
     1861 ctgctcttcg catcgaagga actgatgccc gcagccctgg agatactggg ggtgaaatag
     1921 attgcgccta gccgtggtcg ccagggtcct ctcgcttttg tgcgcgataa tttctctctt
     1981 catgttcttc ccgatcatct ggtctcttac agatggcagt tccgacctcc agccgcttgc
     2041 gatcgggatg ctctccggtt tttccctttc ggtcctcctt ttcctttgcg ggatcaacgc
     2101 caaggcaaag gatctgggtg taagggaggc atttgccgta gtctcactct cgtgggtact
     2161 ggcatcggcc atagggggag tgccatatct ccttcacggg tctgttccga cttttactga
     2221 cgcatttttt gaggccatgt ccggtttcac aacgactggc gcctcggtcc tggccgatat
     2281 ccagtccaac cccagaggga tccttttctg gaggagtctg acccactggc tgggcgggat
     2341 gggaataatc gtactcagcc tggccatact gcccttcctg ggtgtaggag gaatgcagct
     2401 ctacaaggcc gaagttcccg gcccgatccc cgaaaaaatt actccccggg tgcagcagac
     2461 cgccatcctg ctctgggggg tatatgttct tctctcagct ctccagacgg cggccctgat
     2521 gctgggtggt atgaatctgt tcgaatccct tacacacact ttcggtacca tggccacagg
     2581 tgggttctcc cctctcaacg gaagcattgg acagtatggg aacgcctatt tcgagtgggt
     2641 aatcaccctt ttcatgttcc ttgcgggggt caattttgtg ctccatctcc ttgtgcttcg
     2701 aggaaacccg gccgcgctct gtcgagatga agagtttcgt ttctacggga aaatagctct
     2761 tgccgcgatc ttggccacaa cggtttcgct ctttctggcc gggacttatg aaaacattag
     2821 tgacgcattc cgttacggca ccttccaggt gatcagcatc ctgaccacta caggttatgt
     2881 gacggcggat tatgaaaaat ggccattttt tatacagtat cttcttctct tcctcatgtt
     2941 cgtgggtggc tgtgcgggct ccaccggcgg tggcatcaaa aacatgagaa ttatggtaat
     3001 gtcccgtcat gtgggggctg aattgcagag gctgcttcac cccagggcga cagtacccgt
     3061 cagagtgggg ggcagagtgg tcgaaaggga gataatctcg tcggtgacgg cctttttcgt
     3121 aatgtacata gggctgtttg ctctggccgc catggcaatg tcagccctgg gtctggacgt
     3181 gctgacggcg atagcaagtg tcgcggctac cataggcaac ataggcccgg gattggggac
     3241 cgttggtcca acggaaaact atgctcatat tccggcggcc ggcaaatggg tgctctcttt
     3301 ctgcatgctc ctgggaaggc tcgagatcta tacggtaatg atgatcttcg ttcccggaac
     3361 atggagaagg taggaagacc cagggggcgt gagaggtgag aggtgaggag ggaaaagcct
     3421 ggaggcaagt gctttgtcat tctgagccta gcgaagaatc cgggttgcgt tgtgttttga
     3481 actttcgatt cacgaaaaaa cagatccttc gctgcgctca ggatgacaac aaaatcaact
     3541 gcgtaaacgg tgaacgg
//
LOCUS       DAOTIQ010000110         3363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_33467_length_3363_cov_7.620314, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000110 DAOTIQ010000000
VERSION     DAOTIQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33467_length_3363_cov_7.620314"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1181
                     /locus_tag="P5541_09155"
     CDS             30..1181
                     /locus_tag="P5541_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRV72191.1"
                     /translation="MKAVQIGGGLVGQLIVADMMKDFDVTVVDMNETALREIEKKYPG
                     VKTAAASATDPAAIGPVLEGADIVTAGVPGRFGFEMMKTVIGLGKNLVDISFMAEDFE
                     ELDDLARTKGVTVIPDMGVAPGMSNFLMGRGAALLDEVEDAKIYVGGIPTRLVPPFNY
                     QVTWSPKDCIEEFTRPVTIVTDYEKQVVEATYGLHLRDFAGVGTLEAFYTDGLRSLAK
                     NIKARNLGEMTLRWPGHVEQMRLLRSMGLFDETPRDLGGKEVVPLDVAADLLFPMWKM
                     DPEKGDRDLTVMEIEVHGYKGADEMTHSWYLLDYFDEESWNTSMSRCTGCSCSIFARA
                     VAKGLVKTKGVLPAEKLAADDTLYEYVIKEQKKRKIIYRESVKKIINVR"
     gene            1605..3113
                     /gene="nhaC"
                     /locus_tag="P5541_09160"
     CDS             1605..3113
                     /gene="nhaC"
                     /locus_tag="P5541_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRV72192.1"
                     /translation="MSAKQQVKPGLGLSIGVFVAAAFIISYGVLKLGVDAHIPIVFSA
                     VLVCIVGLTVLKMPWSQIEEGAINAIAVALQAIVILMIIGMVIGIWIQSGVVPSLIYY
                     GLDILAPSIFLLATLLITSVVSISTGSSWTTSGTVGIALMGIAHGLGIPAPLTAGVVI
                     SGAYFGDKMSPLSDTTNLAPAVAGATLFDHIRAMIWSTGPTYLIVAAICVVLGMNYAG
                     GSIDAEKISAMQQMMKAEFNIGLLGFLPPILVIGLAVRKTPAIPGLFAGVLVAALMSA
                     FQGNGLGDIIDAVHYGYGATVSAEIAGAEGDALMNLLSQFNLSIAPEMATEVGEMLDS
                     LVSRGGLDSMMWTISLIFCALFFGGAMEACGFLETIVGSITNKVKSVGGMMGAVIGTC
                     FLANLFLGDQYLSLVIPGRMFKSAFEDKGLAPRMLSRAAEDAGTLTSALIPWNTCGAF
                     QSSVLGVPTLEYLPYAFLNYLNPIVSVAMSYMKIGIYWRKADGTDVVAREKP"
BASE COUNT          744 a          874 c          925 g          820 t
ORIGIN      
        1 attgtcgcgc tcgaaaaggg ggattatcta tgaaagcggt acagataggc ggaggccttg
       61 tcggacaact tatcgttgcc gacatgatga aagatttcga tgtgaccgta gtcgacatga
      121 acgagactgc gctcagggag atcgagaaga aatatcccgg ggtaaagaca gctgcagcat
      181 cggcaaccga tcctgcggcc atcggcccgg tccttgaagg tgcggacatt gtcacggcag
      241 gggttcctgg cagattcggc ttcgagatga tgaagaccgt catagggctt ggaaagaacc
      301 tcgtggatat ctctttcatg gccgaggatt ttgaggaact cgatgacctg gccaggacga
      361 agggtgtcac ggtgattccg gacatgggtg tcgctccggg catgtcaaat ttcctgatgg
      421 gcaggggcgc ggcactgttg gacgaggtcg aagacgcgaa gatctatgtt ggtggcattc
      481 ccaccaggct tgtacccccc ttcaactacc aggtcacatg gtcccccaag gattgcatcg
      541 aggaatttac ccgtcctgtg acgatagtaa ctgattatga aaaacaggtc gtcgaggcca
      601 catacggtct gcacctcagg gattttgcgg gtgtggggac cctcgaggcc ttctacaccg
      661 atgggctcag aagccttgcg aagaacataa aggccaggaa tcttggagag atgaccctca
      721 ggtggccggg acatgtcgaa cagatgcgtc ttctgcgttc catggggctt ttcgacgaga
      781 ctccaaggga tctgggcgga aaagaagttg tccctcttga tgtggccgcc gaccttcttt
      841 tccccatgtg gaagatggat cccgaaaagg gtgacaggga tcttacggtt atggagatag
      901 aggtccatgg ttacaagggc gccgacgaga tgactcattc ctggtacctg ctggattatt
      961 tcgacgagga aagctggaac acttccatga gccgctgtac gggctgctct tgctccatat
     1021 ttgcccgtgc cgtcgcgaag ggcctcgtaa aaacaaaggg tgttcttccg gcagaaaaac
     1081 tggcggcaga cgacaccttg tacgaatacg tcataaagga gcagaaaaag agaaagatca
     1141 tttacaggga gtcagtaaaa aaaataataa atgtcaggta ggcaagggtt tctcaaagat
     1201 tatcccgctg atccgataaa tgatgtataa ttatgatgaa agcaaataat gtccgtcagg
     1261 attacaccgg tttatttatt tccctgcttt cattgtggag gtgcgggtat ggacccggtt
     1321 ggcgtcttct tctcattcat tttttgtgaa cctgttcgat gggccggttc ccttttctct
     1381 atcggctgtg tagtcatgtc caggtatctc gatattagcc tgtttgcgag tggtcgtgcc
     1441 cgctgaagaa aaggcctccc tgaaaagagg tcttttttgt tattatgcag taccatgaac
     1501 cgagcggaca aaataaaagg gggtgttagg aaagaaactt gacagggcat caggaaaggg
     1561 aagctccatc atcaagacaa tctgaaaatg gaggaggtaa ttccatgtca gcaaaacagc
     1621 aggtcaagcc aggcctgggg ctttcgatcg gagtattcgt agccgcagcg tttattatca
     1681 gttatggtgt tctcaagttg ggtgtggatg cacacatccc gatagtcttt tccgccgtcc
     1741 tcgtatgtat agtgggcctc accgtcctca aaatgccgtg gtcccagatc gaagaaggtg
     1801 caattaacgc catcgcggta gctcttcagg ccatcgttat cctgatgatc ataggtatgg
     1861 tcatcggtat ctggatccag agcggagtcg tgccgagcct tatctactac gggctggata
     1921 ttcttgcgcc ctcgatcttc cttctggcca cgctccttat tacgtccgtg gtctccattt
     1981 ccacaggctc ttcctggacg acttccggaa cggtcggcat agccctgatg ggtatagccc
     2041 acggcctggg tatccccgca ccccttaccg ctggtgtggt tatttccgga gcctacttcg
     2101 gggacaagat gtcgcccctc tctgatacca ccaaccttgc cccggccgtc gccggagcta
     2161 cgctgttcga ccatatccgg gccatgatct ggtccacggg cccgacctac ctcatcgttg
     2221 ccgccatctg tgttgttctg ggaatgaact atgccggcgg ttcgatcgat gcggagaaga
     2281 tatccgcaat gcagcagatg atgaaagccg agttcaacat cggccttctc ggtttcctgc
     2341 ctccgattct cgttataggc cttgccgtca gaaagacccc ggctatcccc ggacttttcg
     2401 ccggtgtcct tgttgccgca cttatgtccg ctttccaggg caacgggctt ggcgacatta
     2461 ttgacgccgt ccactacgga tatggcgcaa ccgtttccgc agagatcgca ggcgcagagg
     2521 gcgatgccct tatgaacctc ctgagccagt tcaatctcag catcgctccg gagatggcca
     2581 cagaagtcgg tgaaatgctt gacagcctcg tctcacgcgg tggtcttgac tcgatgatgt
     2641 ggacgatctc gcttatcttc tgcgcccttt tcttcggcgg tgcaatggaa gcctgcggtt
     2701 tcctcgagac catcgtcgga tccatcacca acaaggtcaa gagcgtcggc ggaatgatgg
     2761 gagccgtaat tggtacctgc ttcctggcaa acctcttcct cggtgaccag tacctgtcac
     2821 tggttattcc gggcaggatg ttcaagagtg ccttcgagga caaggggctc gctcccagga
     2881 tgctttcccg tgcggctgaa gatgccggaa ctcttacttc ggccctcatc ccctggaaca
     2941 cttgcggagc cttccagagc agcgttctgg gagttcccac ccttgagtat cttccctatg
     3001 ctttcctgaa ctatctcaac ccgatagtct ccgtagcgat gtcctacatg aagataggca
     3061 tttactggag gaaggcagac ggcacagacg tggtcgcgag ggagaaaccg taacccgcgc
     3121 aattgtctcg cagctttttt tgatcgcaac aagaggaggg cggggttttc cccgcccttt
     3181 ccctttcccc cgtttaccgt tcaccgttca ccgttcacgc ctttaggtct tcgttgtcat
     3241 tctgaggacc agcgggacga agaatctgac cccaagacct tctcaccgca gtggtcgcag
     3301 agggtggggc cccgcagaga aaaccattct ttgtgagaac cggggaccta agatttgtca
     3361 ttc
//
LOCUS       DAOTIQ010000111         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_35741_length_3196_cov_5.651385, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000111 DAOTIQ010000000
VERSION     DAOTIQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35741_length_3196_cov_5.651385"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="P5541_09165"
     CDS             complement(<1..362)
                     /locus_tag="P5541_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72193.1"
                     /translation="MSKESVLLLFDENRPFWNDRLVDMVGEDPTVLRDLAVEGLLTRV
                     EEGYCLTGMGRDRFQKWAAESWLESVPGEVPPDPQLEGMKLETALLFERGFRGFQGTK
                     QVTVSPKLEIFPDIPPEEL"
     gene            complement(381..1826)
                     /gene="cls"
                     /locus_tag="P5541_09170"
     CDS             complement(381..1826)
                     /gene="cls"
                     /locus_tag="P5541_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRV72194.1"
                     /translation="MSSGVFFILLHDISSVTIRLVMLCLVPQWHNPSAAMAWLLVIFF
                     WPIPGLALYLVFGSYRLPRERIFRHEEILRTLDRNCCAIHEGERPRDVDLPPELLRFA
                     LLGEKLGDMPPTRGNRTGLIDSAEAFAEAVASDIEAARHHVHLLYYIFASDAVTKPVI
                     DALERASSRGVSCRLLVDALGSGKFLKKEAHSLRDAGVQVVGALPLGMFRSNPISARF
                     DLRNHRKLAVIDGRTGYMGSHNMTEPSYGGKAGGLLWKDLTLRVEGPEVLQLQRVFLE
                     DWFVETNDLLDEKEGLPLFIHDGESILQTVPSGPSYRTENYQRLVVTALHSAMREVVI
                     TTPYLIPDDSLMEGLEVAVLNGASVKLIIPERSDQFFVGNAAKAYYDMLMGRGVEIFL
                     YDDGVIHAKTMTVDGNLCFFGSSNFDIRSFALNFELNCILYGEGPTLLIREAQMKFLN
                     RSKLLSAEEWGKRQFIFRPIEGIAKLMSPLL"
     gene            complement(1823..2608)
                     /locus_tag="P5541_09175"
     CDS             complement(1823..2608)
                     /locus_tag="P5541_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRV72195.1"
                     /translation="MFLLAGKGLRLVLYNTRYCTGNGWRYHLPFPFAGTLRNTCVRSG
                     DVTGYIASLEPDIVGLVESDGGSWRCGGISQPEDLAGRLGCQCIFSRKYRHSRLTERI
                     PVLRHQGNAVVTGLPLSGTCVHRLRRGIKNLVIEATFDDFVFLLVHLSVGPGARRRQI
                     LELADIAGGRSKPVLLAGDFNTFGGDRELEPLLRAGFEKAGPARCPTFPSSRPRLGLD
                     MVLRSTGVSVTSFSVPEVIYSDHLPVICDFRIDSLQGEGGCGP"
     gene            complement(2652..>3196)
                     /locus_tag="P5541_09180"
     CDS             complement(2652..>3196)
                     /locus_tag="P5541_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRV72196.1"
                     /translation="LSDDILVQLIFGTLLGATGMAIVFNQNASTGGTDITAKILNKYF
                     NIDIGKGVLACDFFIVLAAGWVFGMRIGMYAMLGVILNGFLVDNVIIGLRINKYVVIV
                     SRASEDIRRFIVNDLGSGATIYEGRGGWSGERKEVITTIVNRRKFIRLRGFIKALDPD
                     AFISVNNIHEVLGEGFERLA"
BASE COUNT          755 a          935 c          827 g          679 t
ORIGIN      
        1 agttcttctg gagggatatc cgggaatatc tccagttttg gggaaaccgt aacctgtttc
       61 gtcccctgga agcctctgaa tccccgctcg aaaagcaaag ccgtctcgag tttcatgccc
      121 tccagttgcg gatcaggcgg cacttctccc gggaccgact caagccagga ttccgcagcc
      181 cacttctgaa acctgtcacg ccccatccca gtcaggcaat atccctcttc aacccttgtc
      241 agaagtcctt caacggcaag atcgcgcaga accgtcggat cctctccgac catatcgaca
      301 agcctgtcgt tccagaaggg tctgttctca tcgaaaagaa gcagtacgct ctccttgctc
      361 acacacctca ctcctttcct ctacagaagg gggctcatca gttttgctat cccctcgatg
      421 ggtcggaaaa tgaactggcg ttttccccac tcctccgccg acaaaagttt cgacctgttg
      481 aggaacttca tctgcgcctc ccggatcagc agggtcggcc cttcaccgta gagaatgcag
      541 ttcagttcaa aattgagggc gaaggacctg atatcgaagt tgcttgatcc gaagaaacag
      601 agatttccgt ccaccgtcat ggtttttgcg tgtatcaccc cgtcatcata gaggaatatt
      661 tctacccctc tgcccataag catatcgtag tatgcctttg ccgcgttgcc tacaaagaac
      721 tggtcggatc tctctggaat gatcagcttt accgatgctc cattcagaac cgccacctca
      781 agaccctcca tgaggctgtc gtccgggatg aggtagggag tcgtaatgac gacctccctc
      841 attgccgagt gcagggctgt gaccacaagc ctctggtagt tctccgttcg gtacgatggc
      901 ccgctcggaa ccgtctggag aatgctttct ccgtcgtgga tgaaaagagg aagcccctct
      961 ttctcatcga gaaggtcatt ggtttcgacg aaccagtcct ccaggaagac cctctgcagc
     1021 tggaggacct cggggccttc aacgcgaagg gtcagatcct tccacagcag ccccccagcc
     1081 ttgccgccgt atgacggctc cgtcatgttg tgggagccca tgtagccggt cctgccatct
     1141 atgacagcga gcttgcggtg atttctcagg tcgaagcggg cagagatcgg gttactcctg
     1201 aacatgccca gaggaagggc accgaccacc tgcactcctg catccctgag ggagtgggct
     1261 tccttcttca gaaattttcc tgaaccaagg gcatcgacaa gcaacctgca cgagacacct
     1321 ctcgaggaag ccctctcaag agcatcgatg accggcttcg tcacagcgtc ggaggcaaaa
     1381 atgtagtaga ggagatggac gtggtggcgg gcggcttcga tatcggaagc taccgcttcc
     1441 gcgaaggctt cggcggaatc tatcagtcct gtccggtttc cacgagtcgg tggcatgtcg
     1501 ccgagttttt ctccgaggag cgcgaaacgc aacagttccg gcggaaggtc cacgtcacgg
     1561 ggacgctccc cttcatgaat ggcgcagcaa ttgcggtcca gggtcctgag gatctcttca
     1621 tgccggaata tcctctccct gggaagtctg tacgagccga agacaaggta gagcgcaagt
     1681 cccgggatcg gccagaaaaa tatgaccaga agccatgcca tggcagccga agggttgtgc
     1741 cattggggga ccaggcagag cataaccagc cgtatggtca ccgatgatat atcgtggagc
     1801 agtatgaaaa acacgccgga actcatggcc cgcaaccacc ttccccttga aggctgtcaa
     1861 tcctgaaatc gcagatcacc gggaggtgat cggaatagat cacctccggg acgctgaagg
     1921 aagtcacgga aaccccggtc gagcgcagga ccatgtcaag acccagcctt ggtctgctgc
     1981 tggggaaggt gggacagcgg gcaggacccg ccttttcgaa cccggcccgg agaaggggct
     2041 ccagttctct gtctcctccg aaggtattga agtcacccgc gagaagcaca ggtttgctcc
     2101 gccccccggc gatatcagcc agttcaagaa tctggcggcg tcttgctccc ggtcctacag
     2161 agagatgaac caggagaaat acaaagtcgt cgaaagtggc ctctatgaca aggtttttga
     2221 tccctcttcg cagcctgtga acacaggtcc ccgataacgg aaggcccgtg actaccgcgt
     2281 tgccctgatg acggagaacg ggaatgcgct ccgtcaagcg tgagtgccgg tattttcgtg
     2341 aaaagataca ctgacaccca agccttccag caagatcctc aggctggctt attcctccac
     2401 acctccagga tcccccatcg gactctacca gaccgacgat atcgggctcc agggatgcga
     2461 tgtagccggt gacgtcgcca gaacgcacgc aagtattacg gagagtccca gcgaacggga
     2521 aaggcagatg gtacctccac ccatttcctg tgcagtacct ggtattgtag agtacgagcc
     2581 ttaacccttt gccagccaga agaaacacct ccagcccgaa ttgtaatcca tcgacagagg
     2641 ataaaaacag ctcatgcgag tctttcgaaa ccctccccca ggacctcatg aatgttattg
     2701 acacttataa aggcatcagg gtcaagagcc ttgatgaaac cgcgaagcct gatgaatttg
     2761 cgccggttta cgatggtggt aatgacctcc ttgcgctctc ccgaccatcc gcctcgacct
     2821 tcgtagatag ttgccccgct gccgagatca ttcacaatga aacgacggat atcctcgctt
     2881 gccctgctga caatgaccac gtatttattg atcctgaggc cgattataac gttgtcgacc
     2941 agaaaaccgt tgagtatcac gccgagcatc gcatacatgc ctatcctcat cccaaaaacc
     3001 caccctgcgg cgaggacaat gaaaaaatcg cacgccagaa ctcccttgcc aatatcgata
     3061 ttgaagtact tgttgagaat ctttgccgtg atatcggtac cacccgtgga ggcgttctgg
     3121 ttaaagacta tcgccatccc tgtagccccg aggagggtcc cgaagataag ctggacaagt
     3181 atgtcatcag acaggg
//
LOCUS       DAOTIQ010000112         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_37046_length_3110_cov_7.689362, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000112 DAOTIQ010000000
VERSION     DAOTIQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37046_length_3110_cov_7.689362"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="P5541_09185"
     CDS             <1..570
                     /locus_tag="P5541_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRV72197.1"
                     /translation="EMGNDSEPLPEEPGLFLKGANSLADPGLDIPYPHFTNEFHYEGE
                     LAVVIGSRILTGTGDPMKHVLGYTAGIDLTARDRQRKDLQWTRAKAADAFCPLGPWIE
                     TDIDPSSTVVRTFVNGAMRQEGRTSDMIFPVPVILKYITAFMTLEAGDVVLTGTPEGV
                     GELFPGDRVEVTVEGVGAPLVMTVSGEGA"
     gene            577..2685
                     /locus_tag="P5541_09190"
     CDS             577..2685
                     /locus_tag="P5541_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRV72198.1"
                     /translation="MVIGKRDIPDIGHLLSPRSVAVVGASGNLESISGRPVKLLQRFG
                     YKGKIFPVNPGRDVISGLECFPDVSSLPETPDVVLVGVRASLVPGVMEQCAKRKVPFS
                     VIFSSGFAESEDIDSQKRIREIAASGGVRILGPNCQGIVNFADSIPLSFSASLDSDSR
                     PLGGVAYVSQSGAFGFASFAMAAEKGVGFRYVVTTGNQADLDVVDFGMAFVNDPEVNL
                     VVLYMEGLSDGSRFTEMLRFAGERDVPVAVLKVGKSPTGRVAAMSHTAALTGEDEAWR
                     TIFRQYGVIEIEDSEDIADLGKMVGRRTRVSGKRLGIVTTSGGAGIIMSDRAWDMGLD
                     VEPLPDDVQKSLGRYVPSFGSTANPVDLTAQIINEPEGFSACLEAVLGCPDVDMVAVL
                     VSMITGDSGAHVTKKIIEAARATDKPLACCWLINREQGGAFLDELRESGVPLFRSPKR
                     CVWALSQLVRWKYRPRLPSRVPEDLKKPFLPELPPELTEYDSKQLLSRWGIPVTREIL
                     VSSLDCAISAAGEIGYPVALKVVSPDIIHKTEAGAVALRLRDEEVLRNAYGRLLERST
                     QAVPDAKIRGVLIQEMVSGGVECMIGVKRDPLFGPLVAVGLGGIFVEILGDISLRQAP
                     VDREEAMEMVRSLRGYSLLAGVRGSTPVDVEALAEMVSKISLMACAEETLAELDVNPV
                     FVLEKGAVAVDAIAIRSEGA"
     gene            2682..>3110
                     /locus_tag="P5541_09195"
     CDS             2682..>3110
                     /locus_tag="P5541_09195"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRV72199.1"
                     /translation="MNEFFWVIAGYLFGSVPAGFLAVRALLGRDIREMGSGNTGATNV
                     SRFLGKKWGVAVALFDMAKGGIVVLAARMCGVQDPWILAATGLAGVFGHNFPLWLRFR
                     GGKGVATSYGVIFFWNPLPALVGGAVWYLVIRFSGYVSVGS"
BASE COUNT          635 a          785 c          984 g          706 t
ORIGIN      
        1 gagatgggca acgattccga acccctcccg gaggagcccg ggcttttttt gaaaggggcc
       61 aactcccttg cggatcctgg tcttgacatc ccctatccgc attttacgaa cgaatttcat
      121 tacgaggggg aactggcggt tgtgatcgga agccggatcc ttaccgggac cggtgatccc
      181 atgaaacacg tcctgggtta cacggcgggg atagacctga cggcgcgaga caggcagagg
      241 aaagacctcc agtggacccg tgccaaggct gccgatgcct tctgtcccct gggaccatgg
      301 atcgagacag atatagatcc ttcttcaacg gtcgtcagga ccttcgttaa cggcgcgatg
      361 aggcaggagg gacgaacctc ggacatgatc ttccctgtgc cggtgatcct gaaatacatc
      421 actgccttca tgaccctgga ggcgggtgac gtggttctga cgggaacgcc cgagggagtt
      481 ggagaacttt tcccgggtga ccgggtcgag gtgaccgtcg aaggtgtcgg agcgccgctc
      541 gttatgacag tttccggaga gggagcctag ttcggcatgg taatcggcaa gagagacatc
      601 ccggatatcg ggcacctttt gtccccccgt tcggttgccg tggtcggggc ctcggggaat
      661 ctggagagca tctctggtcg ccctgtcaaa ctgctgcagc gtttcggcta caagggaaag
      721 atctttcctg tcaatccggg acgggatgtt atctcgggcc tggaatgttt ccccgatgta
      781 tcttctcttc cggagacgcc tgacgtggtt ctggtggggg taagggcaag cctcgtgcca
      841 ggcgtcatgg aacagtgcgc caaaagaaag gttccctttt cggtaatatt ttcctcgggt
      901 ttcgcggaga gcgaggacat tgactcgcag aaaaggataa gagaaattgc cgcaagcggc
      961 ggtgtgcgga tactggggcc caattgccag gggatagtaa atttcgcgga ttcgattccc
     1021 ctgagttttt ccgcttctct ggacagtgac tcacgccctc ttgggggtgt ggcctatgtt
     1081 tcccagagcg gggcctttgg atttgcttcc tttgccatgg ccgcggaaaa gggcgtcggt
     1141 ttccgctacg ttgtcaccac cggtaaccag gccgaccttg atgtggttga tttcggcatg
     1201 gcctttgtga acgacccgga ggtgaacctt gttgtcctct acatggaagg tctgtccgat
     1261 ggcagcaggt tcactgagat gctgcgtttt gcaggggagc gtgacgtccc ggtcgctgtt
     1321 ctcaaggtgg gaaaaagccc cacggggcga gtcgcggcca tgagccacac cgctgccctg
     1381 acgggagaag acgaggcctg gagaaccata ttcagacagt acggtgttat cgagatagaa
     1441 gactctgaag atatcgccga cctcgggaaa atggtcggcc gccgaaccag ggtttcggga
     1501 aaaaggctgg gtatagttac cacatcaggt ggagccggca taattatgtc ggacagggca
     1561 tgggatatgg gtctcgatgt tgaacccctt ccggacgacg tgcagaaatc tctcggcagg
     1621 tatgtcccgt ctttcggatc cactgccaac ccggtggacc tcaccgccca gataataaac
     1681 gagcccgaag ggttcagcgc ctgccttgag gctgtcctgg gatgcccgga tgtggatatg
     1741 gtcgccgtgc tcgtatccat gataaccggc gattcgggag cccacgtgac caaaaaaata
     1801 attgaggccg ctcgggcaac tgacaaaccc cttgcctgct gctggcttat caaccgtgaa
     1861 cagggtggag ctttccttga cgaactgcga gagagcggtg ttccactttt cagaagtccc
     1921 aaaaggtgcg tatgggccct ttcacagctt gtcaggtgga aatacaggcc cagacttcct
     1981 tcccgggtcc ccgaagacct gaaaaaacct ttccttccgg aactgccgcc ggaactgacg
     2041 gaatacgact ccaagcaact gctttcgagg tgggggatcc ccgtcacaag agaaatcctt
     2101 gtatcatcac tcgattgcgc gatatcggcc gccggagaga taggttatcc cgtggctctc
     2161 aaggtggtct ccccggacat aatccacaag accgaggccg gagccgtcgc gttgcgtctc
     2221 agggatgaag aagttctgag aaatgcctac ggaaggcttc ttgaaaggtc gacccaggcg
     2281 gtgccagacg cgaagataag aggggttctt atacaggaga tggtttcagg cggggtagag
     2341 tgcatgatag gagtgaaaag agatcctctc ttcgggcctc ttgtggctgt cgggctgggc
     2401 ggaatctttg tcgagatact tggagatatc tccctgaggc aggctcccgt cgacagggaa
     2461 gaagccatgg aaatggtaag gtctcttcgc gggtattccc tcctggctgg agtaaggggc
     2521 agcactccgg tggatgtgga agctctggca gagatggtgt cgaaaatatc gctgatggcc
     2581 tgcgccgaag aaacccttgc agaactggat gtcaaccctg tttttgttct cgagaagggt
     2641 gccgttgccg ttgatgccat tgccataagg agtgaagggg catgaacgaa tttttctggg
     2701 tgatcgcagg gtaccttttc ggatccgtgc cggcggggtt tcttgcggtc agggcgctcc
     2761 ttggcaggga tatcagggaa atgggttcag gcaacaccgg ggcgacaaat gtttcccggt
     2821 ttctgggaaa gaagtgggga gtggcggttg ccctctttga catggccaag ggtggaattg
     2881 tcgtactggc cgcaaggatg tgtggtgtcc aggatccctg gatactcgcc gcgacgggtc
     2941 tggccggagt gtttggacat aattttcccc tgtggctgcg tttcaggggg ggcaagggcg
     3001 tggcgacctc ctacggagtg atcttcttct ggaatccgct tcccgccctt gtcggtgggg
     3061 cggtctggta cctggtgatt cgtttctcgg ggtacgtttc ggtaggttcc
//
LOCUS       DAOTIQ010000113         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_37547_length_3079_cov_8.593915, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000113 DAOTIQ010000000
VERSION     DAOTIQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37547_length_3079_cov_8.593915"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1010
                     /locus_tag="P5541_09200"
     CDS             177..1010
                     /locus_tag="P5541_09200"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRV72200.1"
                     /translation="MVYRSVFISDVHLGTRWSKAEELASFLSSIRCEKLYLVGDIIDG
                     WKLRNNPRWPQSHNRVIRKILKMAKRSEVFYITGNHDEFVDEFEGYDFGGIRICKKTS
                     HITADNRRFMVIHGDEFDVVVKYRKWLAFLGDTAYTFALYLNDILTWIRSRLGLPYWS
                     LSQYLKHRVKDAVAFVGDFEETLLAETRKGNHHGVICGHIHRPTIRNIEDTVYCNSGD
                     WVESCSALVEHADGTLEVVRWDGEKIIGDVCERPKLLEMDYVVSPDTNNCPEKRVPLA
                     V"
     gene            complement(1041..2075)
                     /locus_tag="P5541_09205"
     CDS             complement(1041..2075)
                     /locus_tag="P5541_09205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRV72201.1"
                     /translation="MRIAIVTDAWTPQINGVVNSIRNTVDCLENKGHLVSVISPELFR
                     TVPCPTYPQILLAVDVWFVGKYLEEFCPDAVHIATEGPIGLAARQWCLARSFPFTTAY
                     MTRFPEYISMRFRIPQDLLTGYFLWFHSPSSGVMVSTASLRDELAARGFRNLKMWSRG
                     VDTEKFHPATFGIDLMKNLPRPRFLYVGRVAVEKNIKAFLDLDLPGSKIVTGDGPALD
                     ELRESCPGVVFTGARTGRELASIYACSDVFVFPSLTDTFGIVMLEAAACGLPVAAFPV
                     QGPKDVVIEGVTGVLDEDLQKACMEALGIDRETCREHALSCSWGSCTEQFLENLQVLS
                     AGERPGSLAS"
     gene            2588..>3079
                     /locus_tag="P5541_09210"
     CDS             2588..>3079
                     /locus_tag="P5541_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV72202.1"
                     /translation="MLAGKKILAAMAFLFFISLLPIASGGGERSCMEVARLFEGEKPL
                     EWGQEVSGVITRFEPGDRKIVALTFDACGGGLKGNGYDGELVEFLRKEKIPATIFVNA
                     RWIRSNPDIFAELASDPLFEIANHGLEHKPLSVSGKKAYGISGTASPAEAAREIEGGA
                     EAIE"
BASE COUNT          809 a          816 c          804 g          650 t
ORIGIN      
        1 atcctggcct ctgaacctct tctgctggaa aagaccagaa aatcctgcag gtccctggca
       61 agacccgatg catccaggat catagcccat gaggtaataa gaagatccat caatcccgcc
      121 tgtctcaggc cggaaacggc ccctcccgca gagacggctg aaacaaggtg ataaaaatgg
      181 tctacagaag cgtgttcatt tccgatgttc atcttgggac aagatggagc aaggcagaag
      241 aacttgcatc cttcctttcg tcgataagat gcgaaaaact ttacctggtc ggcgacatta
      301 tcgacgggtg gaagctcagg aacaacccgc gctggccaca gtctcacaac agggtcatca
      361 ggaaaatcct taaaatggcc aaaaggtctg aagttttcta cataacagga aaccacgacg
      421 agttcgtcga cgagttcgag gggtacgatt tcggcggcat caggatctgc aagaagacat
      481 cccacatcac agccgataac aggcggttta tggtgattca cggagacgaa ttcgatgtgg
      541 tggtcaaata ccgaaaatgg ctggcttttc tcggggacac ggcctacacc ttcgcgctgt
      601 accttaacga tattctgacc tggatcaggt cccgtctggg tctcccttac tggtctcttt
      661 cccagtatct gaaacatcgt gtcaaggatg cagttgcctt tgtgggagat ttcgaggaaa
      721 ccctcctcgc ggaaacccgg aaggggaacc atcacggggt aatatgcgga cacatccacc
      781 gtccgacaat acgaaacata gaggataccg tctactgtaa ttccggagac tgggtagaga
      841 gttgttcggc acttgtggag catgccgatg gaaccctgga agtggtcagg tgggacggcg
      901 aaaagattat tggagatgtc tgcgaaaggc ccaaactcct tgaaatggat tacgtggttt
      961 cccccgatac gaacaactgc cccgagaaac gggttcccct ggcagtatga actctgacaa
     1021 agacaaaagc gcgggagggt tcaggaggcg agagaccctg gtctctcgcc cgcggacagt
     1081 acttgaaggt tttccagaaa ctgttcggta catgaacccc acgagcatga aagggcgtgt
     1141 tccctgcagg tttcccgatc gataccaagg gcttccatgc aggctttttg cagatcctca
     1201 tcaagaacgc cggtaacccc ttcaatcact acatcctttg gcccctggac cggaaaagcc
     1261 gccacaggaa gcccgcaagc ggcggcctcg agcataacga tcccgaatgt gtcggtaaga
     1321 ctggggaaaa caaagacatc gctgcaagca tagatggaag caagttccct ccccgttctg
     1381 gcaccagtaa aaaccacccc gggacaggac tccctgagtt catcaagggc cgggccatca
     1441 ccagtaacga tctttgatcc tggcagatcc aggtcaagaa aagccttgat attcttctcc
     1501 actgccacac gtccgacgta aaggaacctg ggcctgggaa ggtttttcat cagatctatc
     1561 ccgaaagttg cgggatggaa tttttctgtg tctactcccc tggaccacat tttcagatta
     1621 cggaaccccc gggccgccaa ttcgtcacga agcgatgcag tggataccat tacccccgaa
     1681 gagggagaat ggaaccagag gaaatatccc gtcagtaaat cctggggaat tcggaaacgc
     1741 atcgagatat actcggggaa ccgtgtcatg taagcagtag tgaacgggaa agatctcgca
     1801 aggcaccact gtcgcgccgc caggcctata ggtccttcgg tagcaatgtg taccgcgtcg
     1861 ggacaaaatt cctcaagata ttttcccaca aaccatacat ccaccgccaa aaggatctga
     1921 ggatacgtcg ggcaaggaac tgtcctgaaa agttccggtg aaataacaga gaccaggtgt
     1981 cccttatttt caagacagtc gacagtgttc cggatggagt tgacaacccc gttgatctgg
     2041 ggagtccacg catcggtaac gatagctatc ctcatcgctc tcccccctgt tccgggcact
     2101 ttgatccccc gccaatcgcg ggggccatgc cacccctgaa aaaatcctac tattcctttc
     2161 ctacgaaata gtttcattac tgtaaccatc ctgttgcgaa actgcatctc cccttgtttt
     2221 tgatttgtaa cataaatgtt aggctccggt gacgctgccg tagcagatcc ggggttacga
     2281 tgactttgct tttttaggag gttatgcgat gaactgcgcg ggggcactaa tcggagagat
     2341 agccgaggag gtcgccatga gggcctcaaa cacaaggtga aacaacacag tggggaactt
     2401 ggcaaaagta catatctttt cgacaggaga gaccaccagt cttcaaaaca ggaggactgc
     2461 ccccaataat ccgatgcaag ccggttctgc ccactcagtg accgcggtcg ccccgaaaac
     2521 tgacaccctt acccgcaagg acattcaaag tgtaaaatac ccccggtcaa tgaaagaggt
     2581 gaaaaatgtg cttgcaggga aaaagatcct tgctgcaatg gccttcctgt ttttcatatc
     2641 cctgctcccg atagcatccg ggggaggaga gcgctcgtgt atggaggtcg ccagactttt
     2701 tgaaggagag aaacctctcg agtgggggca ggaggtctcc ggtgtaatta cccgattcga
     2761 accaggcgac agaaagatcg tcgccctcac cttcgatgcc tgcggaggtg gcttgaaggg
     2821 caacggctat gacggggagc ttgttgaatt cctgagaaag gaaaagatcc ccgcgaccat
     2881 tttcgtcaac gccaggtgga taagatctaa cccggatatt tttgccgaac tggcttcgga
     2941 ccccctcttc gagatagcca accatggcct ggaacacaag cccctttctg tcagcggcaa
     3001 aaaggcttac ggcatatctg gaacagccag cccggctgag gcagcccggg agatcgaggg
     3061 cggtgcagag gccatcgaa
//
LOCUS       DAOTIQ010000114         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_39019_length_2993_cov_8.464602, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000114 DAOTIQ010000000
VERSION     DAOTIQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39019_length_2993_cov_8.464602"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1067
                     /locus_tag="P5541_09215"
     CDS             123..1067
                     /locus_tag="P5541_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV72203.1"
                     /translation="MARQLRIEYPGALYHVTSRGNEKKDIFRSIKDREKFLSYLSLAW
                     ERHGAVFHAYCLMSNHFHLMVETPLGNLSWIMKHINGSYTTYFNVKHKRVGHLLQGRY
                     KAVLVQADTYAAELSRYIHLNPVRAKMVPSPEEYQWSSCRRYLEGTEPSWLSTSLVLG
                     YFGTEFENRRRNYRDYLYEAIGKESRDPLADSAASTILGNDDFVRDIREKYLDGKEKD
                     RELPALRDLTRGPDIADIKAVSEEAFPEDSRLARMAGIYLCWRFSGAKLKEIGDLYGI
                     SESGVNRSCDRLESRLEKDRHLEEELQQMAENLKKSKV"
     gene            complement(1285..2457)
                     /locus_tag="P5541_09220"
     CDS             complement(1285..2457)
                     /locus_tag="P5541_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV72204.1"
                     /translation="MKIKMGQGLRYVVQWGFLVFLLSLAYLHDRYGGGVTGFPTVCSI
                     CPFGGMETLYAWFSAGTFIRRTSPADLVLLFGFIGITLILGRTFCGAICPLGTVQEWM
                     TKGGRALGIRARELPSSIDRPLRFLKYGVFLAIIFFTWSAGELIWRDYDPWVALMHVF
                     EPLELVLEKPIGLAVLVGIVLIGTIFIERAWCRYFCPLGALLSVFHFLSPLKVRRNQE
                     SCIDCGLCDLSCPVKLDISDRETVNSPECIQCGKCTDACPVPKTLEIGTKKGSRFSYL
                     KILVATLLIFLVVYGISRATGHWMTYAPMTQTTRSVESPAPLPGDGIQGSAKLETLAE
                     HFGLDAETILEKAGWPLDVPRDRSLREIMTERPELDLSIRSIRDAVNDLLKQTGTE"
BASE COUNT          764 a          737 c          845 g          647 t
ORIGIN      
        1 gggtattcag cctcttgtgc cttgatctga gctatttggg caacaggccg tacacttttg
       61 actaaatgtt gaaggtgagg gaagatattg agagacactt tcccggacag gaggttcccc
      121 ccttggccag gcagttgagg atcgaatacc ccggtgcttt gtatcatgtt acatcgcgag
      181 gcaatgagaa gaaggatatc ttcaggagca taaaagaccg cgagaagttc ctgtcctacc
      241 tgtcattggc ctgggagagg catggagcag tctttcacgc ttactgtctt atgagcaacc
      301 acttccacct catggtggag acccccctcg ggaacctctc atggatcatg aagcacatca
      361 acggatccta caccacttat ttcaacgtca agcataaaag ggttgggcac cttttgcagg
      421 gccgctacaa ggcggtgctg gtccaggccg atacctatgc cgcagagctt tcccgctaca
      481 tccacctgaa tccggtcagg gcgaagatgg tcccatcccc agaagaatac caatggagca
      541 gttgtcgccg ataccttgaa ggcacagagc catcctggct ttcgacaagc cttgtccttg
      601 ggtatttcgg cacggaattt gaaaaccgcc gaaggaacta cagggactat ctgtacgagg
      661 caatcgggaa agaaagccga gaccccctag cggattcagc ggcctcgacg atcctcggca
      721 acgatgactt tgtcagggac atcagggaga agtacctgga tggtaaggaa aaagaccgtg
      781 aattgccggc tttgagggat ctgacccgtg gtccagacat agcagatata aaagcggttt
      841 cagaggaggc cttcccggag gactccaggc tggcgaggat ggcggggatt tatctctgct
      901 ggcggttcag cggcgcgaag ctgaaggaga tcggggacct ctacggtata tctgaatcgg
      961 gggtaaaccg ttcctgcgac aggcttgaga gcaggttgga aaaggacagg catctggaag
     1021 aagaacttca acagatggcg gaaaacctga aaaagtcaaa agtgtaggtg tgacccctat
     1081 ggctgctccc aatgggtcca gccgatagaa agcccgtttt taactcgcta tcgatctttc
     1141 gtaacttccg gagtgaccgg ggctgttcta aaggacgtag aggtcaatta aaatttgaat
     1201 taaaaaaggg cggggccgtt gatctgcggc ctcgcccttt ctgttgcagg ctgatcatgg
     1261 tgcttgtgct cctgagggtt ccgatcactc cgttcccgtc tgttttaaaa gatcgttcac
     1321 ggcatcccgg atgcttcgaa tgctaaggtc gagttcgggt cgctccgtca tgatctcccg
     1381 gagggacctg tccctgggta catccagggg ccacccggcc ttttcgagta tggtttccgc
     1441 atccagtccg aagtgttccg ccagtgtttc cagtttggcg cttccctgta ttccgtcgcc
     1501 gggaaggggc gcagggcttt cgacagatct ggtggtttga gtcatggggg cataggtcat
     1561 ccaatgtcct gttgcccgcg agatcccata gaccacaaga aaaatcaaca acgtggctac
     1621 aaggatcttg aggtaggaga aacgggaccc cttttttgtt cctatttcga gtgttttcgg
     1681 tacagggcag gcatcggtgc actttccgca ctgaatgcat tcgggagaat tcaccgtttc
     1741 cctgtcactg atgtcgagct tcacagggca tgagaggtcg caaagaccgc aatcgatgca
     1801 gctttcctgg tttcggcgca cctttaaggg agacaggaaa tgaaagaccg aaagcagggc
     1861 gcccagaggg cagaagtatc ggcaccaggc cctttcgata aaaatggttc ctatcaggac
     1921 gatcccaacg aggacggcaa ggcctatggg tttttcaagg acaagttcca ggggttcgaa
     1981 aacatgcatg agggctaccc aggggtcgta atcgcgccag atgagttctc cggccgacca
     2041 ggtgaagaaa attatggcca aaaacacacc atactttaga aaccggagtg gcctgtctat
     2101 agaggagggc agttcgcggg cccggatccc cagggcgcgt ccgccctttg tcatccactc
     2161 ctggaccgtg cccagggggc agatagctcc gcagaaggtt ctgcccagga tcagtgtgat
     2221 cccaataaaa ccaaagagga gcaccagatc tgccggcgat gtccttcgaa tgaatgttcc
     2281 ggctgaaaac caggcgtaaa gggtttccat tcccccgaaa gggcagatgg agcagacagt
     2341 agggaaccct gtaacgcccc cgccgtaacg gtcatggaga taagccaggc tcagaagaaa
     2401 aactagaaac ccccactgga caacgtaacg aagcccttga cccatcttta tcttcatctg
     2461 tcacatcccc catcccacag gatttccatc gttcatgatc aacggtgcgc tttaatccat
     2521 gccttacgag actttaccat ggatagaaaa gacgatacag caaaaggtca agaccagacc
     2581 atccgcttta ttacggaaaa attatttgtt ctgtgaagga gggccggatc gcgaaaactt
     2641 acaagagtca gatcgaacac gcttgaaaaa cattctttgg ggcactttgc ggatatggcc
     2701 aggttggtaa ggagatcggg aaaccatacg ggatatccga atagggcgta atccgtgccc
     2761 tgaggcaggc ctgagcccgc ccaaaggggt tagacgtggg aagtgagacg aaaaggcgag
     2821 gcggcaggag actggaggca ggaaactgga agggcaagaa caagggcaaa atcaaaggcg
     2881 tgaacggtaa acggtaaacg gtcaacggtg aacgggaagg gcaaatgctt tgtcattcgg
     2941 tagtgcttat tagtttgtgt aaacctccat tgtcggggag aattgatatc tct
//
LOCUS       DAOTIQ010000115         2898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_40686_length_2898_cov_6.429476, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000115 DAOTIQ010000000
VERSION     DAOTIQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40686_length_2898_cov_6.429476"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1791
                     /locus_tag="P5541_09225"
     CDS             406..1791
                     /locus_tag="P5541_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV72205.1"
                     /translation="MPERTGPPYDSTQALEGISRDVVFEHSGAANMVVASDTTLLSVN
                     SRFEELTGYSKGEVEGKMSWTKLVHPEDLPRMIDNHRLRRENPSLVPPSYEYRFMTAD
                     GGIRWGLLTGATLANGSRTLISVVDVTPLKEKEAALSRTEVFYKDIVQTQTELIMRFT
                     PDLVLTFVNDAYCRYYGETRESLLGRSFICHALPEDVEGQRKYFRGITPDIPVREIEE
                     RTILPSGEIRWQHWLDKGLFNRVGELVEIQSSGRDITDLHRTREKLDATIEKLKNSFA
                     LSIEMAGKIIEVRDPFTAGHQRRVARLAKAIATEMSLDSDSVTATELAALAHDVGKIH
                     IPSEILSKPGMLQPAEREIIRQHPRYSAEILQILETPWNIARIALQHHERIDGSGYPD
                     GLKGDDISIEARILAVADVIEAMSSHRPYRAGFGMEIALNEIEAGRGKRYDSDVTDAA
                     LTLLREKEYSL"
     gene            1788..2561
                     /locus_tag="P5541_09230"
     CDS             1788..2561
                     /locus_tag="P5541_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRV72206.1"
                     /translation="MKNSLRSRLAAGEVVIGPFAGLNCPDLVEIMGIAGCDFCVIDTE
                     HGPMDPESIQHMVRAAEVAGLSPIVRVPRAEAVDILKILDIGAHGIHVPQVNSRQGAV
                     DVVKAARYHPEGIRGVAVPRALSYGLRPLGEALAEANREIQVIVHCENVDGLEKLDEI
                     ASVEGVDLVFVGPYDLSQSLGVPGDIFHPSMVEAVSRALRLILEAGKPAGIFVTSTDE
                     AKRRIDEGFRYIAYSMDSLIFGSACREAVSILRSHRQGT"
     gene            complement(2527..>2898)
                     /locus_tag="P5541_09235"
     CDS             complement(2527..>2898)
                     /locus_tag="P5541_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside reductase class II"
                     /protein_id="HRV72207.1"
                     /translation="NEVNGRPVEVFTTMGKSGHETMAFTEAMGRLISLALRSGVKIHG
                     IIKQMKGIGGSQPVWHDNGVIMSVPDAISYGLMSMGYLDLEEKGMMESLSETDMCPVC
                     GASMARQEGCIKCPACGFSRC"
BASE COUNT          694 a          764 c          806 g          634 t
ORIGIN      
        1 tgatgtaata tttgccgccc cgcaaggggc ggtttttttt taagactcgt gaagcgtgat
       61 gcgtaactcg aaagcccagg acctcgtgaa tcggagggct gtcattctga ggaatgaaat
      121 gacgaagaat ctggtttagg taccgtgagt cgtaaatcgt gaatcggtta aagcaaaaac
      181 agatccttcg cttcgctcag gatgacaagc tcttgccttt gctttttcga ttcacgactc
      241 acgattcacg actcacgaaa aaaacagatc cttcgtttcg ctcaggatga caaaaaccaa
      301 aggcgtcaac ggtaaacggt gaacggaagt gcaaggcgtc tggacggctt cggttttacc
      361 gtttaaaatt aaagtgtaat acatcaaact tcaggatgtg gattgatgcc ggaacgaaca
      421 ggccccccct acgactctac tcaggctctt gaaggaatat ccagggatgt tgttttcgag
      481 cactccggag ccgccaacat ggtggtcgct tccgacacga cccttctgtc tgttaacagc
      541 cggtttgagg aacttacggg ctactcaaag ggagaggtcg aggggaagat gagctggacg
      601 aagcttgtcc atcccgagga tcttcccaga atgatcgata atcatcgcct ccggcgcgaa
      661 aacccttccc tcgtaccacc aagttatgaa taccgcttca tgaccgccga cggcgggatc
      721 agatggggtc tgcttaccgg ggccaccctg gcaaacggct caaggaccct catatcggtc
      781 gtggacgtga cccccctcaa ggaaaaggaa gccgccctct cccggacgga agttttctat
      841 aaagacattg tgcagaccca gacggaactg atcatgaggt tcaccccgga tcttgttctc
      901 accttcgtaa acgatgccta ctgccgatat tacggagaaa ccagggagtc cctcctcgga
      961 agatccttta tctgtcatgc tcttcccgag gatgtggaag gacaaagaaa atatttccgc
     1021 ggcataacgc cagatattcc tgtccgggag atcgaggaga ggacgatcct tccctcaggt
     1081 gagatacgat ggcagcactg gcttgacaag ggtctcttca acagggttgg cgaactggtc
     1141 gagatccagt catcggggag agacattaca gatcttcacc ggacaaggga aaaacttgat
     1201 gctacgatag aaaaactcaa gaactccttt gctctttcca tagagatggc ggggaagatt
     1261 attgaggtca gagatccatt cacggctgga catcagcgga gggtcgcaag gctcgcgaag
     1321 gccatagcca cggagatgag tcttgacagt gactcggtaa ctgcaacgga actggccgct
     1381 cttgcccacg atgtgggaaa gatccatatc ccgagcgaga tcctcagcaa gccgggcatg
     1441 ctccagccgg ctgaacggga aataatcagg cagcatccaa ggtacagtgc tgaaatactg
     1501 cagattcttg agactccctg gaacattgcc cggattgctc ttcagcatca tgagcgcatc
     1561 gatggcagtg gttatcccga tgggctgaag ggcgacgata tcagtataga ggctcgcata
     1621 cttgcagttg ccgatgtgat agaggccatg tcctctcatc gtccctaccg cgcgggtttc
     1681 gggatggaaa ttgccctcaa tgagatcgag gccggaagag gcaaaagata cgacagcgac
     1741 gtgacggatg cggcgctgac acttttaagg gaaaaggagt acagcctgtg aaaaattctc
     1801 taaggagcag actcgccgcc ggcgaagtgg ttatcggtcc atttgcgggg cttaactgtc
     1861 cggacctggt cgagataatg ggtattgccg ggtgtgattt ttgtgtaatt gacactgaac
     1921 acggcccgat ggacccggaa tcgatccagc acatggtccg tgctgccgag gtcgccgggt
     1981 tatcccccat cgtaagggtc ccccgtgccg aagcggttga tattctcaag atcctggaca
     2041 taggagcgca cgggatacat gttccccagg tcaacagccg acaaggcgct gtggatgtcg
     2101 taaaggccgc aagataccat cccgaaggca ttaggggagt cgctgtgcca agggctcttt
     2161 cctatggtct gaggcccctt ggggaagctc tcgcggaggc taacagggaa atccaggtca
     2221 tagtccactg cgagaacgtt gacggactgg aaaaacttga cgagatagca tctgtggaag
     2281 gtgtggacct ggtttttgtc gggccctatg atctgtcgca gtctcttggg gttccggggg
     2341 atatcttcca tccatccatg gtggaagcag tgtcgcgggc cctccgcctt attctggagg
     2401 caggaaagcc cgcgggtata ttcgttacct cgacggacga ggcgaaacgc cgcatcgacg
     2461 aggggtttcg ttatatagcc tacagcatgg acagccttat cttcggcagc gcctgcaggg
     2521 aggccgtcag catcttgaga agccacaggc agggcacttg atgcacccct cctggcgggc
     2581 catcgacgcg ccgcagacag ggcacatatc cgtctccgag agggattcca tcatcccctt
     2641 ctcctcgaga tccagatacc ccatgctcat cagcccgtag gagatggcat cggggaccga
     2701 cattattacc ccgttgtcat gccatacggg ctgggaaccc ccgataccct tcatttgctt
     2761 tattatcccg tggatcttga ccccgctcct cagggccagg gagatcagcc gacccatcgc
     2821 ctcggtaaag gccattgtct cgtgccccga tttacccatc gtggtgaata cctccacagg
     2881 gcgtccgttc acctcgtt
//
LOCUS       DAOTIQ010000116         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_42641_length_2799_cov_6.621356, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000116 DAOTIQ010000000
VERSION     DAOTIQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42641_length_2799_cov_6.621356"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1287)
                     /locus_tag="P5541_09240"
     CDS             complement(211..1287)
                     /locus_tag="P5541_09240"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRV72208.1"
                     /translation="MNSSYADTEWLQSLPGFPRLQLALLLPTPLHRLQRFGEHIGGID
                     LWMKRDDLTGLAGGGNKTRKLEFIVGDAIRQGADTLVTVGAIQSNHTRQTAAAANRAG
                     LACALLHCGWTGDAGLFYRRAGNILLSSLLGAELFLDEKERPIEDQGPLDVLFEHLRS
                     RGQKPYLISGGASDQPYGGLGYAVCAAEMTVQAASMGIHIDYVVHCTGSSSTQAGLVA
                     GFAAMGSKTRVIGIPDDEETDIKSGRVLRLANQTLEELDRPERVGRENIEIVVGDESP
                     YGKSDEKTYRSIRLFSEKEGLIADPVYEGKAIRGLIELAEKGRFEKGAVVVLLHMGGT
                     PAVHAYADTFGPIEFKSIPGRRTL"
     gene            complement(1773..2570)
                     /locus_tag="P5541_09245"
     CDS             complement(1773..2570)
                     /locus_tag="P5541_09245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRV72209.1"
                     /translation="MRERKQVTAAAIFKDGKVLVAQRGPKDKLANKWEFPGGKVEGNE
                     TPEACLQREIKEELGLEIKVGEYLCSSFYDYDYISIELMAFACETVSGEPNNNEHQKL
                     IWVDPSQLINLDMAPADLPIVERIQKERGQFFDSIGGTGQMSGTNSKFKYLYRDASNY
                     KQFGYVVFRGEPTPKDSECVIGNLHEGDFFIPEVVGLQSLQEKFESVSIDDHPWHEID
                     FRTETERSPFELTSEEPTDARSVHRFAEEFAKTKWDEMQASRRMGLI"
     gene            complement(2594..>2799)
                     /locus_tag="P5541_09250"
     CDS             complement(2594..>2799)
                     /locus_tag="P5541_09250"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HRV72210.1"
                     /translation="FRGSKWTSINKEERKTYLKNAYRVLLTRARQGMVIVVPPGDPED
                     LTRIPEFYNPIYDYLESIGFDQI"
BASE COUNT          615 a          755 c          636 g          793 t
ORIGIN      
        1 ccggtaaaga atccggcagt gacagtccga gtcttgaagc caagcctttc gagggtctgt
       61 tctctttttt caaacgttct caggcaaatc cgtcatgctt gcattcgaca actggttacc
      121 tgctccgtct gatctgcctg gactaaccgg ggagactcaa ttgggcaaca gcccgtgtag
      181 gtctgacact tatagggttc tggtctgaac ttatagggtt cttctgcccg gaatcgactt
      241 gaactctatg gggccaaagg tatcggcata agcatgaacg gcaggagttc ctcccatgtg
      301 gagcagaacg accactgcgc ctttttcgaa ccgccccttt tcggccagtt caataaggcc
      361 tctgatcgcc ttaccttcat agaccggatc agctatcaag ccctcttttt ccgagaacag
      421 ccttatggaa cggtaggtct tttcatcgga tttgccatag gggctttcgt cacccaccac
      481 tatctcgata ttctccctgc ctactctttc gggtcggtcc agttcttcaa gggtctggtt
      541 ggcaagccga aggacccgtc cggactttat atctgtctcc tcgtcatcgg gaattccgat
      601 aacccgggtc tttgaaccca ttgctgcaaa gccggcaaca agcccggcct gggtgctact
      661 gcttccggta cagtggacca cataatcaat atgtatcccc attgaggctg cctggaccgt
      721 catctccgcc gcacagacgg cataacccag gcccccgtat ggctggtccg aagctccgcc
      781 tgatataaga tagggttttt gccccctgga acggaggtgc tcgaacagca catcaagtgg
      841 gccctgatcc tcgataggac gctccttttc atcaagaaaa agttccgcgc ccaggaggct
      901 gctaaggagg atgttccccg ccctcctgta gaacagcccc gcgtcccctg tccacccgca
      961 gtgaagcaat gcacaggcaa gaccggcacg gttggctgcc gcggctgtct gtcgggtgtg
     1021 gtttgactgg atggcaccga cagttacaag ggtgtcggcg ccctgcctta ttgcatcgcc
     1081 gacaatgaat tccagcttcc tggtcttgtt gccacccccg gcgagacccg tgaggtcatc
     1141 cctcttcatc caaaggtcga tcccgcctat atgctcacca aacctctgaa gccgatgaag
     1201 cggtgttgga agaagaagcg ccaactgtag gcgcgggaaa ccgggtagcg actgaagcca
     1261 ctccgtatct gcataagatg aattcatggc agcctccttc tggaaaaacc ttctcaagat
     1321 cgtaaaagaa ccgattttga agcgatatta aacgaaggat gcatgaccgg tcaaacggat
     1381 cgacagatcc gggaagatgg gcaattcatg cttttgttcc agttcagcct gacgatcttc
     1441 ctggcagggt gtttattgac tccaacttct aatgtctcct cgcttatttc tataacaact
     1501 tcgctaaggt cagtatcact ccttcagcac caaatgaaat taagaatatg atgcattcag
     1561 gtcgtttatc actgatattt ccctttttcc agcagttttg tgaattattc aggtctggat
     1621 cccatggatc caccccgttc ctttttgcct acacgtcccc tttggttgcc cttttctgca
     1681 tcctgccggc aggcaaccag tcttcgttcg ttttgttgcg gagatcttct catcccccgt
     1741 gcccggtgtg aaaaaagacc ttgattttca ggtcagatca accccatcct tcggctggct
     1801 tgcatctcat cccatttggt ttttgcaaat tcttcagcga atctgtgcac gcttctggca
     1861 tcggttggtt cttcacttgt aagttcaaaa ggagatcttt ctgtctctgt cctgaagtcg
     1921 atctcgtgcc atgggtggtc gtcaatactg acgctctcaa atttttcttg gagggattgg
     1981 agaccaacga cctcgggaat gaagaagtct ccctcatgaa ggttgccaat tacacactcg
     2041 gagtccttgg gggtaggctc tcctctaaat accacgtatc cgaactgctt atagttcgag
     2101 gcatccctgt acagatattt gaatttgctg tttgtgccac tcatttggcc agtacctcca
     2161 atactgtcaa aaaactgtcc gcgttctttc tgaatcctct caacaatagg cagatccgcc
     2221 ggggccatat caagatttat caattgtgat ggatctaccc aaatcaactt ctgatgctca
     2281 ttgttattgg gctctccgga aacagtctca cacgcaaaag ccattagttc aatggatatg
     2341 taatcgtaat catagaacga agaacatagg tactccccca ctttgatttc taatcccagt
     2401 tcttctttta tctctctctg gagacaggct tcgggggttt cattaccctc aactttgccg
     2461 ccaggaaatt cccatttgtt ggcgagttta tccttgggtc ccctttgtgc cacaaggact
     2521 ttcccgtctt tgaagatcgc tgcggcggta acttgcttac gttctcgcat tagttctcct
     2581 tttttgattg tggctagatt tgatcaaacc cgatactttc caaataatca tagattggat
     2641 tgtaaaattc aggaattctt gtgaggtctt ctggatcacc cggaggtact actattacca
     2701 ttccttgtct agctctcgtt agcagtactc tgtaggcatt ttttagatag gttttgcgtt
     2761 cttctttgtt gatacttgtc cacttgctac cccggaagg
//
LOCUS       DAOTIQ010000117         2590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_47302_length_2590_cov_4.007101, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000117 DAOTIQ010000000
VERSION     DAOTIQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47302_length_2590_cov_4.007101"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="fusA"
                     /locus_tag="P5541_09255"
     CDS             <1..869
                     /gene="fusA"
                     /locus_tag="P5541_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRV72211.1"
                     /translation="VAVKPRSRADEDKLANALNKMLEEDLTLHFRKDPETGDSVLSGM
                     GDLHIDVMLSKVKERYGVDLDTETPRVPYRETIKKTSKAQGKYKKQTGGRGQYGDVHI
                     EFVPLARGEGFKFEDKIVGGSVPKSYIPAVEKGLKEAILKGVLAGYPCVDFKASLFFG
                     SYHDVDSSEMAFKIAASMAYKKGIVEAAPVILEPIMNIEVVVPEDYLGDVMGDFNSRR
                     GKIMGIDSRGRLQVVKAQAPLAEMFRYAIILRSMTSGRGNFTMEFSHYEEVPHDISKK
                     IITEADVQDEED"
     gene            complement(944..2527)
                     /locus_tag="P5541_09260"
     CDS             complement(944..2527)
                     /locus_tag="P5541_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV72212.1"
                     /translation="MTNDYESIKHADTILVIGSNTTENHPVIGSWIKEQKKQGISKLI
                     VCDPRRIELDHYADIKIGHRSGSDVALLNGLMNIIIAEELYDKDFVEKNVEKFDELKA
                     LVAEYTPKKVSTITGVPEETLKDAARTYAKGPNSAIFYTMGITQHKYGTNNVRTVANL
                     ALLCGMLGRPGTGVNPLRGQNNVQGACDMGCLPAVLPGYLNIAKKTEEAVEKVRKLWN
                     AKMPTNPGKTIVKIMEGASTGEIKALFVMGENPMVSDPNTNHVRHALENIDFLVVQDI
                     FLTETAQMADVVLPASCWAEKDGTFTNTCRAVQRIRKAVEAPGEARPDWEIIQNIAKA
                     CGASWNFASPAKIMDDIARFVPQYGGVSYERLEKGPLMWPCPDANHPGTPNLYTTGFP
                     RGKASFVPCEWNKPHEWPDVEFPFIATTGRTLYHYHTGTMTRRTPSAEFIKSLYVEIN
                     PADAKEMNVVQGDNIRVTSRRGSVVGEARVTEKVPPKMVFVPFHFGEQPANALTASVW
                     DIDTETPAFKINAVKVEKE"
BASE COUNT          537 a          671 c          728 g          654 t
ORIGIN      
        1 gcgttgcggt caaacccaga agccgggcgg acgaagacaa acttgccaat gccctgaaca
       61 aaatgctgga agaggatctc accctgcatt tccggaagga tcccgagacg ggggacagcg
      121 tgctctctgg aatgggcgac cttcacattg atgtcatgct ctccaaggtc aaggaacgct
      181 acggagttga ccttgataca gagactccga gagtcccata ccgtgaaacc atcaaaaaga
      241 cgtccaaggc acagggcaag tacaagaagc agacaggagg aagagggcag tacggtgatg
      301 tccatatcga atttgtgccc cttgcccggg gcgaaggatt caagttcgag gacaagatag
      361 tcgggggatc agttcccaag tcctacatac ctgctgttga aaagggcctc aaagaggcga
      421 tactcaaggg tgttcttgcg ggttatccct gtgttgactt taaggccagc ctctttttcg
      481 gctcatacca cgatgtcgac tcttctgaaa tggccttcaa gatagcagcc tcgatggcat
      541 acaagaaggg gatcgttgag gccgctccgg tgatactcga accgataatg aacatagagg
      601 ttgtagttcc cgaggattat ctgggcgatg tcatgggaga tttcaatagc cgcaggggaa
      661 agataatggg catagacagc cgcggcaggc tgcaagtggt caaagcccag gccccgctgg
      721 ctgaaatgtt caggtatgcc attattctgc gctccatgac ctcgggtcgt ggcaatttca
      781 cgatggagtt ctcccattac gaggaggttc cccacgatat ttccaaaaag atcattaccg
      841 aggccgatgt tcaggacgaa gaggattagt acctgaaaaa gcgttgacta tcgggtatca
      901 aaaaagaggg gccccgcggg gcccctcttt catttaacgg atcctattct ttttctacct
      961 ttacggcgtt gatcttgaat gcaggtgttt ccgtatcgat gtcccacacc gatgccgtga
     1021 gggcgttcgc gggctgctct ccgaagtgga aaggaacgaa gaccatcttc gggggaactt
     1081 tttcagttac ccgagcttca ccgacaacgc ttcctcgcct ggaggtcacc ctgatattat
     1141 ccccctggac tacattcatt tctttcgcgt ctgccgggtt gatctcgaca tagagggact
     1201 tgatgaactc tgcggagggc gttctccttg tcatcgtgcc ggtatggtag tggtagagcg
     1261 tccgccccgt ggtagctatg aaggggaatt ccacgtcggg ccattcatgg ggcttgttcc
     1321 actcgcatgg cacaaaggaa gcctttccgc gggggaaccc tgtagtgtag aggttcggcg
     1381 tcccggggtg gtttgcatcg gggcacggcc acatgagcgg gcccttttcc agcctttcat
     1441 aggagacccc gccgtactgc ggcacgaacc tggctatgtc atccattatc tttgccgggg
     1501 aagcaaaatt ccacgatgct ccgcaggctt tagctatatt ctggatgatc tcccagtcag
     1561 gccttgcttc cccgggagct tctacggcct tgcggatcct ctggaccgct ctgcaggtgt
     1621 tggtgaaggt cccgtccttc tctgcccagc aagaggcagg caggacgaca tcggccatct
     1681 gggcggtttc ggtcaggaaa atatcctgaa ccaccaggaa gtcgatattt tccagagcgt
     1741 gacggacatg gttcgtgttg ggatcgctga ccatcgggtt ctcgcccatt acgaaaaggg
     1801 ccttgatctc ccctgtcgac gctccctcca tgatctttac aatggttttg ccggggttgg
     1861 tcggcatttt tgcgttccag agcttcctga ctttttcaac tgcctcttca gtttttttgg
     1921 ctatgttgag gtagcccggg aggactgccg ggaggcatcc catgtcgcat gctccctgga
     1981 cgttgttctg cccgcggagc gggttgacgc cggtgccggg ccttccgagc attccgcaaa
     2041 ggagggccag attggcaacg gtccttacgt tgttggtacc gtacttgtgc tgggtaatcc
     2101 ccatggtgta gaagatggcc gaattggggc ccttcgcgta ggttctcgcg gcatctttca
     2161 gtgtttcttc ggggactccc gtgatggtgc tgaccttttt cggtgtgtat tccgcaacaa
     2221 gtgccttgag ttcgtcaaac ttttcgacgt tcttttctac gaaatcctta tcgtagagtt
     2281 cttcggcaat gatgatgttc atcagcccgt taagcagggc tacgtcactt cccgaacggt
     2341 ggcctatctt gatatctgcg taatgatcca gttcgatcct cctggggtcg cagacaatca
     2401 gtttcgatat gccctgtttc ttctgttcct tgatccatga tccgatgacc ggatggttct
     2461 cggtggtatt ggacccgata accagtatcg tatcggcgtg ttttatcgat tcgtaatcgt
     2521 tggtcattgc tccgcttccg agggtctcgc cgagacccgc aacagtgggg ctgtgtcaga
     2581 gatgggcaca
//
LOCUS       DAOTIQ010000118         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_47772_length_2569_cov_5.162689, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000118 DAOTIQ010000000
VERSION     DAOTIQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47772_length_2569_cov_5.162689"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="P5541_09265"
     CDS             complement(<1..410)
                     /locus_tag="P5541_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV72213.1"
                     /translation="MARPSLLRCVLCGREFDPGSLVYTCPDCGLDGTLDVLYDYRKTA
                     EKINRKVLSKQTEKSLWRYREILPVENDAHIPKLSVGWTPIYGSPSLASKYGLKEVFV
                     KDDGRNPTGSLKDRASAVGVAKALDFGQKTVACAS"
     gene            complement(415..1608)
                     /locus_tag="P5541_09270"
     CDS             complement(415..1608)
                     /locus_tag="P5541_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRV72214.1"
                     /translation="MLSEKRKNALVGFCRDIIRIPSPSGAEAGVAGLIEKSMKESGFD
                     EISVDRYGNVTGRIVLGKGGKKILLEGHMDHVDVSDPSKWTHDPFGAELVDGKIYGRG
                     TTDMKGNLVAMVKAAAFVKEDLAGELDGEILVGGSVHEECFEGISCEEIAKQFHPDYV
                     VIGEPSGLTLKRGQRGRAEVLLETFGKNAHSSNPSVGINAVKKMMKAILAIEEGFTPK
                     VHPVLGEGILEVTDIISSPFPGASVVPDRCSATFDRRLLEGENRDDVLGQVRDFLAPL
                     VADDPQMKFSVSLARGEDKCYTGETIEAERFAPGWIFPESEPFVQAALEGLRSVGQDP
                     VVSHYYFCTNGSYYAGKAGIPTVGFGGSLETLAHVDDEYIEVDHLVKACEGYYGIIRS
                     TLGKK"
     gene            complement(1642..>2569)
                     /locus_tag="P5541_09275"
     CDS             complement(1642..>2569)
                     /locus_tag="P5541_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV72215.1"
                     /translation="FKKNGNIYGRGATDQKGSLAAMICAASFLGEDAPAECSGRIVVA
                     ATVHQERAEGASSRLVDINHRPDFVVSGEASALSIERGQRGRAEIVIETLGKTAHSAQ
                     PDFGVNAAEHMVSLISFINKNFRPVSDPFLGEGILELTNLVTSQEKTTGVIPARCRAL
                     FDRRLILGETRETVLGQIEEIIQLCASGLPGLRATARICPAMERCYTGARIEGEHFVP
                     GWLLQRDHSFLLQCLEGLKSVGLEPRVADTAGFGTNGCYYGGVRGIPTIIFGPSFQEL
                     AHITDEYIEVDQLLKGCMGYYGIASAVLSSRK"
BASE COUNT          590 a          773 c          593 g          613 t
ORIGIN      
        1 gaagcacagg ccacggtctt ctgtccaaaa tcaagggcct tggcaacgcc taccgcactc
       61 gccctgtcct tgagagaacc cgtcggattc cttccgtcat ccttgacgaa aacttcctta
      121 agaccgtact tcgaggcaag ggagggagag ccgtaaatgg gagtccagcc gacactcagc
      181 tttggaatat gggcatcatt ctcaacaggg agtatctccc ggtatctcca aaggcttttt
      241 tctgtctgct tcgacaacac tttcctgttg atcttttccg cggttttccg gtagtcgtaa
      301 agaacatcaa gtgtaccgtc cagaccacag tcagggcatg tataaaccag cgatcccgga
      361 tcgaactccc tgccgcagag aacacacctt agcaatgaag gtctcgccat tttgctattt
      421 ctttccaagg gtcgagcgga ttatcccgta gtaaccctca caggccttca caaggtggtc
      481 gacctcaata tactcgtcat ccacatgggc gagggtctcc agtgatcccc cgaaacctac
      541 ggtaggaata ccggccttgc ctgcgtaata acttccattg gtgcagaaat agtagtgaga
      601 aactaccgga tcctgcccta cggaccgaag tccctccagc gcggcctgga caaaaggctc
      661 gctctcaggg aatatccacc cgggggcaaa cctttcagcc tctatggtct cgccggtata
      721 gcacttgtct tctcccctgg ccaaagaaac ggaaaatttc atctgcgggt catcggcgac
      781 aaggggggcc agaaaatcac gcacctgtcc cagaacatca tccctgttct caccctcaag
      841 gagccgcctg tcgaaggtgg cgctgcacct gtcgggaacc acgctggcac cagggaaagg
      901 ggaggaaatg atatcggtga cttccagtat cccctctcca agaacagggt ggaccttcgg
      961 ggtgaaccct tcctctatcg ccagaattgc cttcatcatc ttttttacag cgttgatgcc
     1021 aacagacggg ttcgatgaat gggcgttctt gccaaaggtc tcaagaagga cttccgctct
     1081 tccccgctgc cctctcttca gggtaagacc tgaaggctct cctataacaa cataatccgg
     1141 atggaattgt ttagcgatct cttcgcatga aatcccctcg aaacactctt cgtgcacact
     1201 tccgccgacg agtatctcgc cgtcaagctc accggcaaga tcttccttta caaatgcggc
     1261 ggccttgacc atggcgacga ggttaccttt catgtccgtt gttccccgtc catagatctt
     1321 gccgtcgacc agttcagccc cgaaggggtc atgggtccat ttcgacggat cgcttacgtc
     1381 cacatgatcc atgtgccctt caagaagaat ctttttgccg cctttgccca ggacgatcct
     1441 tcccgtcacg ttcccgtacc ggtcaacaga gatctcgtcg aaaccgcttt ccttcattga
     1501 cttttcgatg agccctgcca caccggcctc agctccggaa ggactgggta tccggataat
     1561 atccctgcaa aaaccaacca gagcgttctt tctcttttct gaaagcaaat cgttgcgcct
     1621 ccctaatatg aattttgcaa tctatttccg actcgacagg accgcacttg cgatcccgta
     1681 ataacccatg caacccttca acaactggtc aacctctatg tattcatcgg tgatgtgcgc
     1741 aagttcctga aaagacggac caaaaatgat cgttggtata cccctgaccc ctccgtaata
     1801 gcatccgttt gtcccgaaac ccgctgtatc cgctacacgt ggttcaagcc ctacgctctt
     1861 caacccttca agacactgaa gcagaaatga gtgatccctc tggagaagcc atcccgggac
     1921 aaagtgctct ccctcaatac gggcccctgt ataacatctt tccatggccg gacatatcct
     1981 ggcagttgcc ctgagtcctg gaagaccgga agcacaaagc tggattatct cctcgatctg
     2041 gccaagcacc gtctcccgag tctcacccag tatcagcctc ctgtcgaaca gggcgcgaca
     2101 ccttgcgggt attacccctg ttgttttttc ctgtgatgtt acaagatttg taagttcaag
     2161 aatgccctct ccaagaaacg ggtcggatac gggtcggaaa tttttattta tgaaagatat
     2221 gagggagacc atgtgttcgg ccgcatttac cccgaaatca ggttgggccg aatgggccgt
     2281 tttgccaagt gtttcaataa cgatctctgc ccgaccacgc tgccctctct ctatggaaag
     2341 ggcggaagct tccccggata caacgaaatc ggggcggtgg ttaatatcta caagcctgga
     2401 agaggccccc tcggcacgct cctggtgaac cgttgcggcg actacaatcc tgccgctaca
     2461 ctccgccggc gcatcctccc cgagaaacga cgccgcgcag atcatggcgg cgagacttcc
     2521 cttctgatcc gtcgcgcctc tcccgtagat attcccgttt tttttgaat
//
LOCUS       DAOTIQ010000119         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_48820_length_2528_cov_4.779620, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000119 DAOTIQ010000000
VERSION     DAOTIQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48820_length_2528_cov_4.779620"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="P5541_09280"
     CDS             <1..471
                     /locus_tag="P5541_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV72216.1"
                     /translation="MIAGILESYLFSFVIMMTVPLSIIGVIPTLIMTGTSLSVYGLLG
                     LVMIVGLVVNNAIVVVDYAEVLRKKGHNAVEAVVEACRVRFRPIIMADVTTLLAMLPL
                     ALGLGAGGQYRAPLAIVLIGGLFAGGTMAVFLIPPIYRLVWDLKGKFQRGDKDA"
     gene            464..1957
                     /locus_tag="P5541_09285"
     CDS             464..1957
                     /locus_tag="P5541_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72217.1"
                     /translation="MRKGLYFWFSVAVAAAIVGGTAYFLGRVPEKTADVARAIPVINM
                     DSPRMYIHAQKGQVEPGFPEKLAGNLDESARPYLRAVDDLLPLFSFAEESAALVAWES
                     NEALFYGSFMVPPDVAKDIGSGKLPDLWMQHSTGLALGPSPGEGFQQLSAGQGQITLT
                     LLVEGNMLFISSSPEGVEKMTAVLKGEEKHFEPRLELEGSWPAHVRIFDGGLISQAVA
                     MRGEKAPETPIDAEIAWKVKGDSGDMAWNVSGASEWIPEDVLSRLKPVEWKKEIVLPD
                     PLIMAAGLSVPEGLETIDADQFEIPGWMKDAGMDRNSLNKLLSGPLLLTVGGQSRAFL
                     FSLPGFLVQLPGRGVSGEKWVESIWNTKWGGFGLSPKTVAGFTHGGMINMPVTVVAAA
                     RDDLAVAGIISSSSLGKLSSPAGSILPPGEKALFWFYADFPRGAEALEQLAKVGGLAG
                     KLGIEGGKDVEELLEMAREMRSLGEVTLIFNDPESGRGSWKGASPLE"
     gene            1997..>2528
                     /gene="tyrS"
                     /locus_tag="P5541_09290"
     CDS             1997..>2528
                     /gene="tyrS"
                     /locus_tag="P5541_09290"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRV72218.1"
                     /translation="MSVNPLAIMMERGCVEWCSHPEELRFLFDNEMVTGYIGFDPTAD
                     SLHVGHLIPLMGLSWMQRSGHRPIAIAGGGTGLIGDPSGKSKERNLLTLEQVDLNLVT
                     VRGQLERFLDFDCGSNSALIINNYDWLGKQSLIEFLRDTGKHFSVNYMVAREYVKTRL
                     DDPEKSISYTEFSYMLL"
BASE COUNT          567 a          621 c          726 g          614 t
ORIGIN      
        1 atgatcgcgg gtatccttga gtcatacctt ttctcctttg tcataatgat gacggtccct
       61 ctttccatca taggagtcat tccaaccctc ataatgacgg gaacgtccct ttcggtctac
      121 ggtttgctgg gtctggttat gattgtggga ctcgttgtca acaatgctat cgtagtagtc
      181 gattatgcgg aggtccttag aaagaagggg cacaatgctg ttgaagcggt agtagaagcc
      241 tgcagagtca ggttccggcc gataatcatg gcagacgtta cgaccctgct ggctatgctg
      301 ccgctggcgc tgggtctcgg agcgggaggg cagtacaggg ctccccttgc catcgtcctt
      361 atcggcggtc tcttcgcggg gggcaccatg gccgttttcc ttatcccgcc gatctacagg
      421 ctcgtgtggg acctcaaggg aaaattccag aggggggata aagatgcgta agggacttta
      481 tttctggttc tccgtcgccg tagcggcggc aatagtaggt ggtacggcct attttcttgg
      541 cagggtgccc gaaaagaccg ctgatgtcgc gagggcaata cctgtcataa acatggactc
      601 tcccaggatg tatattcatg cccaaaaggg acaggtggaa ccgggattcc ccgaaaaact
      661 tgccgggaac cttgatgaaa gcgccaggcc atatctcagg gcggtagatg acctgcttcc
      721 acttttttct tttgccgaag agtcggctgc cctggttgcc tgggaatcca acgaggccct
      781 gttttacggt tctttcatgg tcccgcctga tgtggcaaag gatataggct ccgggaaact
      841 tcccgatctc tggatgcaac actcaacggg gctggccctt ggcccctctc caggggaagg
      901 gtttcagcag ctttctgcag ggcaagggca gataacgctt acactcctcg tggaaggcaa
      961 catgcttttt atctcttcct ccccggaggg cgttgaaaaa atgacggctg ttttaaaggg
     1021 agaagagaaa cactttgagc cccgtcttga actggaaggc tcctggccgg cccatgtcag
     1081 gatcttcgat ggcggtctca tttcccaggc agtcgccatg aggggagaaa aggcacccga
     1141 gactcccatc gatgccgaga tagcctggaa agtcaagggt gacagcggag atatggcgtg
     1201 gaatgtttcc ggggccagtg aatggatccc cgaggatgtc ctctccaggc tcaagcccgt
     1261 cgaatggaag aaagagatcg tcttgcccga ccctctgatt atggctgccg gtttatcggt
     1321 cccggagggg ctcgaaacca tcgatgccga ccagtttgag ataccgggat ggatgaagga
     1381 tgctggtatg gacaggaata gtctaaataa attgctctca ggtcccctcc ttttgaccgt
     1441 tggcgggcag agcagggctt tccttttctc tcttcctgga tttctggttc agttgccagg
     1501 gcgtggtgtt tcaggtgaaa aatgggtgga gagtatctgg aatacaaaat ggggaggatt
     1561 cggtctctcg ccaaaaacag tcgccggctt tactcatggg ggtatgatca acatgcctgt
     1621 cactgttgtg gccgccgcaa gagatgacct tgccgttgca gggatcatct cctcttcaag
     1681 ccttggaaaa ctatcctctc ctgcgggatc tatacttcct cctggcgaaa aggcactttt
     1741 ctggttctac gctgatttcc cccgcggggc cgaagcactg gagcaactgg ccaaggtggg
     1801 aggtcttgcc ggcaaactcg gtatcgaggg aggcaaggat gtagaagaac tgcttgagat
     1861 ggcaagggaa atgaggtctc ttggggaggt aacccttatc tttaacgatc ctgagtccgg
     1921 cagaggaagc tggaagggcg cctcaccgct tgaataaata tcagcaataa atcatacaga
     1981 tctgggagga atataaatgt cagtgaaccc tcttgccata atgatggaac ggggctgcgt
     2041 cgagtggtgc agccaccccg aggaactgcg atttcttttc gacaatgaaa tggtcaccgg
     2101 ttacataggt tttgatccca ctgccgacag cctgcacgtt gggcatctca tcccccttat
     2161 ggggctttcg tggatgcaga ggtcggggca caggcccatt gcgatagcag gcggtggaac
     2221 gggactgatc ggggatcctt cgggcaagag caaggagaga aatcttctta ccctggagca
     2281 ggtcgatctc aatcttgtta cggtcagggg acaactcgaa aggtttctgg attttgactg
     2341 cggcagcaat tctgccctga taatcaacaa ctacgactgg cttggaaagc agtctcttat
     2401 cgagtttctg agagatacgg gcaaacactt cagcgttaat tacatggtcg cccgggaata
     2461 tgtgaaaacc cgactcgatg acccggagaa gtccatttcc tatacggaat tttcctacat
     2521 gcttcttc
//
LOCUS       DAOTIQ010000120         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_50731_length_2456_cov_7.755935, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000120 DAOTIQ010000000
VERSION     DAOTIQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50731_length_2456_cov_7.755935"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="P5541_09295"
     CDS             <1..170
                     /locus_tag="P5541_09295"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163831.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRV72219.1"
                     /translation="GGLAFSEKFVEAVRERVSFIAPVKVYPGEDEMAALAQGALRVLA
                     GEESALEYTTA"
     gene            188..1144
                     /locus_tag="P5541_09300"
     CDS             188..1144
                     /locus_tag="P5541_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV72220.1"
                     /translation="MRIVAFEPLGITDERLKAVAAPLLAAGHTVTQHHDRVEDLMEMI
                     QRAKEADALIVVDQVLPGKLFEACPNIRMVSVASEFRDQVDLQKARAKVVPVSVIPEY
                     WTYSVAELTVALLLAVMRRLTVCNESARGRQVEGCLDGHELFGKTVGIIGTGSVGLHV
                     ARILKAFGCELLGYSRTQKKEAEVWAGMEYVTVEELLERSDIVTLHLPCSPDTESFLN
                     RERLALMKQGAILINTAHGKLVDWHTLAELLKDGWLSGAGIDTLDVEPPIGRSHPLAK
                     APNVVLSPRIGASTPEALEKRAELALANIISWMEGKPKGVVA"
     gene            1172..1789
                     /locus_tag="P5541_09305"
     CDS             1172..1789
                     /locus_tag="P5541_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72221.1"
                     /translation="MSNRVEIKSALEFGWKTFKENALFFIILMAAIGIPMALLNVLMR
                     DLPWFSVSAMFLRGVCWVIRVLAIMAVMLVSLSYRDKGTFDYRNVGALQPLLLPYLMG
                     TLLFSFLVGVGMVLLIAPGVYILVKYQFTPYLLIDKGLQPVEALREAGELARGSWLEL
                     FLFVLAIAGINFLGAILFGIGLLVTIPVTILAQSWVYRSFVPSGN"
     gene            1807..>2456
                     /locus_tag="P5541_09310"
     CDS             1807..>2456
                     /locus_tag="P5541_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV72222.1"
                     /translation="MAGVAVYALFCVALFFFQDSVIYQPRRQIHADPSLVGMDFEEVW
                     FRASDGVVLHGWYVATREPSRRALLFFHGNAGNISHRIESIRIFGQLGLNVFIFDYRG
                     YGSSEGKPSEEGLYADGRAALEALMAKGNEQDEVILFGRSLGGAVAARIASETPVAAL
                     ILESSFTSLAPMASRAAPLFPVRFLLRSKYATQEILPFIEAPLLVIHSPEDEIVPFS"
BASE COUNT          541 a          596 c          703 g          616 t
ORIGIN      
        1 cgggaggact cgctttcagc gagaagttcg tagaggcggt cagggagaga gtctccttta
       61 tcgctcccgt gaaggtctat cccggggagg acgaaatggc agcccttgcc cagggagcgc
      121 tccgggtcct tgccggagaa gagtctgccc tggaatacac taccgcatag acagggaggg
      181 gatttatttg cgcatagttg catttgaacc gctggggatc accgatgaga ggctgaaagc
      241 ggtcgcggct ccgcttttag cggcaggtca tacggtcacc cagcaccacg acagggtgga
      301 agatcttatg gagatgatcc agagagccaa agaggcagat gccctgatag tggtggatca
      361 ggtccttccc ggaaaacttt tcgaggcctg tcccaacata aggatggttt cggtggcttc
      421 ggaatttcgt gatcaggtgg accttcagaa agcccgggcc aaggtggtcc cggtgagcgt
      481 tatacccgaa tactggactt attcggtggc cgaactgacg gtggctcttc ttctggcagt
      541 catgagaagg ttgactgtat gcaatgaatc agccaggggg aggcaggtcg aaggatgtct
      601 ggatggtcac gaactcttcg gaaagaccgt gggaataatc ggaactggtt ctgttgggct
      661 tcatgtggcc aggatactca aggctttcgg atgtgaactt ctcggttaca gccggaccca
      721 gaagaaggaa gccgaggtct gggcaggaat ggaatatgtt actgtcgaag aacttctcga
      781 acgttcggat atcgtcactc ttcatcttcc ctgcagtccc gatacggaaa gtttcctgaa
      841 cagggaaaga cttgccctta tgaaacaggg agctattctg atcaatactg ctcacggaaa
      901 actggtagac tggcataccc ttgcagagtt gctgaaggac ggatggctct ccggagcggg
      961 gatagatact cttgatgtcg agcctcctat cggaagatca cacccgcttg ccaaggctcc
     1021 aaacgttgtg ctttctccca ggataggagc ttccacaccg gaagccctgg aaaaaagggc
     1081 agaactggca ctggcaaaca tcatttcatg gatggaaggc aaaccgaaag gtgtagttgc
     1141 ctgaaaaaac tgtcgagaag ggggctgaac aatgagcaac agagtggaga taaaaagtgc
     1201 gcttgagttt ggctggaaaa ccttcaaaga gaatgctctt ttcttcatta ttctcatggc
     1261 cgctattggt attccgatgg cgctgctcaa tgttttgatg agagatcttc cgtggttctc
     1321 ggtctctgcc atgttcctga ggggcgtatg ctgggtaatc agggtcctgg caataatggc
     1381 agtgatgctg gttagccttt cctacaggga caaggggacc ttcgattatc gcaacgtcgg
     1441 ggctttacag cccttgctcc ttccttacct tatgggaaca cttctttttt ctttccttgt
     1501 aggtgtgggg atggttctgc tgatagctcc aggagtgtac atccttgtaa aataccagtt
     1561 cacgccctat ctgctgattg acaaaggcct gcagcctgta gaggcgcttc gtgaggccgg
     1621 ggaacttgcc cggggctcat ggctggaact cttccttttt gttcttgcga tcgcgggcat
     1681 caactttctt ggggccattt tgttcgggat tggcctgctg gtgaccatac cggtgaccat
     1741 tctggcccaa tcatgggtat acaggagttt tgtaccttca ggaaattgaa tctggcaagg
     1801 atatttttgg caggagtcgc agtttacgca cttttttgcg ttgccctttt ctttttccag
     1861 gacagcgtca tctaccagcc gcggcgacag atccatgccg atccttccct cgtgggaatg
     1921 gattttgaag aagtatggtt cagggcctcc gatggagtgg ttctgcatgg gtggtatgta
     1981 gccacccggg aaccttcccg acgggccctt cttttttttc acggaaacgc cggtaatatt
     2041 tcgcacagga tcgaatcgat ccggatattc ggccagcttg gactaaatgt atttatcttc
     2101 gactatcgcg gatacgggtc aagcgagggg aaaccctccg aagagggatt gtacgccgac
     2161 ggcagggcgg ccctggaagc gctgatggcg aaaggcaacg aacaggatga agtaattctt
     2221 ttcggaagat ctctcggtgg ggctgttgcc gcccgcatag ccagcgagac tcccgttgcg
     2281 gcactgatcc tggagtcttc ctttacatct cttgcgccca tggcgtccag ggccgcgccg
     2341 ctgttccccg tgcgattcct cctgaggagc aaatatgcca ctcaagagat ccttcccttt
     2401 attgaggctc ctcttcttgt gatccacagc cccgaagacg aaatagtccc tttttc
//
LOCUS       DAOTIQ010000121         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_51304_length_2436_cov_7.538849, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000121 DAOTIQ010000000
VERSION     DAOTIQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51304_length_2436_cov_7.538849"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="P5541_09315"
     CDS             <1..401
                     /locus_tag="P5541_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV72223.1"
                     /translation="IVSAYEKGEPVLAYYWEPEWIMGMLDMTMLEEPPYDPDRWGEGK
                     DYGCAFPAARVHIGVNAKFAEKNPLVVEFLKKYETTLAQNNEALAYMQEQEASVDDAA
                     IWFLKNFTDVWKGWLPKDVAGKVETALSGK"
     gene            408..1292
                     /locus_tag="P5541_09320"
     CDS             408..1292
                     /locus_tag="P5541_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163687.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HRV72224.1"
                     /translation="MADNIIEVSELWKVYSKKEIDLDVENEEMIESLDSSSDSVVAVR
                     DVSFSVKEGEVFIVMGLSGSGKSTLIRCLLRLVDISWGSVKINGQEISTMNKKEITDF
                     RRHQAAMVFQHYGLLPHRTVVENVAFGLKLRGVPRKERKEMAREALATVGLQDWGNYY
                     PASLSGGMRQRVGIARALVADTPLLLMDEPFSGLDPLIRREMQEELVRIQEEFHKTIF
                     FVTHDLDEAMRLGDRMAVMKNGEIVQIGKPPEVFANPADDYVARFVQDKREEIRKTDE
                     QMAALATQPEKAVKNDVS"
     gene            1279..2133
                     /locus_tag="P5541_09325"
     CDS             1279..2133
                     /locus_tag="P5541_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HRV72225.1"
                     /translation="MMFPEAFNFSVAKSVNNAIKWLLQTHGSAFDSFSAAILGLLLRV
                     QSLLVAVPWWGYILFIAVMAWIATRKIIPTIVLAGLPIFIGMFGLWALALETLALVIT
                     SVVIALVVGIPLGVIMAEANLFAEITKPVLDAMQTMPSFVYLIPAMMLFGLGKVPAVI
                     ATLIYALPPVMRLTCLGLQQVPKPVQEAARAYGATRWQLLKEVRVPLAMPSILAGVNQ
                     TTMMALAMVVIASMIGARGLGQEVLMAINRIDVGRGFEAGISIVIMGIVIDRITQGLA
                     RRWDPNRK"
BASE COUNT          575 a          595 c          714 g          552 t
ORIGIN      
        1 ctatagtctc cgcctacgag aaaggggagc ccgtccttgc atactactgg gagcccgagt
       61 ggatcatggg tatgctcgac atgaccatgc tcgaggagcc cccctacgat cccgacagat
      121 ggggcgaagg caaggactac gggtgtgctt tcccggcggc tcgggtacac ataggtgtca
      181 acgccaagtt cgctgagaag aacccgctgg tcgtcgagtt cctcaaaaag tacgagacga
      241 cccttgccca gaacaacgaa gcccttgcct atatgcagga gcaggaagcc tccgttgatg
      301 atgcggcgat atggtttttg aagaatttta ccgatgtctg gaaaggctgg ctcccgaaag
      361 acgtggccgg taaagtagaa acggctcttt caggcaagta gacgcgaatg gcagacaaca
      421 ttatcgaagt atcggaacta tggaaagttt attcaaaaaa agaaatagac cttgatgtag
      481 agaacgaaga aatgatcgag tcgctggatt catccagcga ctcggttgtc gcagtcaggg
      541 atgtctcttt ctcggtgaag gagggagagg tttttatcgt tatgggactt tccggcagcg
      601 ggaagtccac cctcattcgc tgtcttctca ggttggtgga tatttcctgg gggagcgtga
      661 aaataaacgg tcaggagata tcgaccatga ataaaaagga gataaccgac tttcgccgcc
      721 accaggcagc gatggtcttc cagcactacg gccttcttcc tcatcgtacg gttgtggaga
      781 acgttgcctt cggactcaag ttgcggggtg ttcccaggaa ggaaagaaaa gaaatggcca
      841 gagaggctct tgctaccgtc ggcctgcagg actggggcaa ttattacccg gcatcactta
      901 gcggaggaat gcgccagcgt gtaggtattg cccgggcact tgtcgccgat accccgcttc
      961 tcctgatgga cgagcccttc agcgggctgg acccgcttat ccgccgcgag atgcaggagg
     1021 aactggtacg tatccaggaa gagttccaca agacgatatt ttttgttacc catgaccttg
     1081 acgaggccat gcggcttggg gacagaatgg cggtcatgaa gaatggtgag atagtgcaga
     1141 tcggcaaacc gccggaagtg ttcgcaaatc cggccgatga ctacgttgcg cgctttgtcc
     1201 aggacaaacg cgaggagata agaaaaacag acgaacagat ggccgcgctc gcgacgcagc
     1261 ccgaaaaggc agtgaagaat gatgtttcct gaagcattca acttttcggt ggccaagagt
     1321 gtaaacaatg ccatcaaatg gcttcttcag acccatggat cagccttcga cagcttctcg
     1381 gcagccatac tgggtctttt gcttcgcgta cagagccttc tggtcgccgt accgtggtgg
     1441 ggatatatcc ttttcatcgc tgtgatggca tggatagcga ctagaaaaat tatacctacg
     1501 atagtcctgg ccgggttgcc cattttcata gggatgttcg gtttatgggc tctggcgctg
     1561 gagactcttg ccctggtaat tacttcggtg gtgatagctc tcgttgttgg cattcccctg
     1621 ggtgtgataa tggcagaggc gaacctgttc gcggagatca caaagcctgt tcttgatgcc
     1681 atgcagacta tgccaagttt tgtctacctg atacctgcaa tgatgctgtt cggactcggg
     1741 aaagtgccgg cggtcatcgc gacgcttatt tacgcgcttc ctcctgtcat gaggctcaca
     1801 tgtctggggc tccagcaggt gcccaagccg gtccaggaag cggcccgtgc ttacggggcg
     1861 acgaggtggc aactcctgaa agaggtcagg gttccgctgg ccatgccatc catccttgcg
     1921 ggagtcaacc agacgacaat gatggcgctg gccatggttg tgatagcgtc catgatcggg
     1981 gcgagggggc ttggtcagga ggtcctgatg gcgatcaacc ggatagatgt cggcaggggt
     2041 ttcgaggcgg gaatatccat cgtcataatg ggtatagtca tagaccggat aacccagggt
     2101 cttgcccgaa ggtgggaccc gaacaggaaa tagatccgtg acgcgtaagt cgtgaattgt
     2161 gaatcgttaa tcgtgaatcg aaaaaagcga ggaagcagga ggctggatac aggaagcagg
     2221 aaaacaaccc ggattcccgc ctgtgggcaa atgacagact tttgcccttc ctgcctccag
     2281 tctcctgttg ccagtctcct tgcttttatt tgtcattctg agcgaagcga agcggaatcc
     2341 gccggtgggc ggacgttccg caccatattc ccaccggcgg taatctggtt caaattccaa
     2401 aaagatactg gctggccctg agtgaagtct tttccg
//
LOCUS       DAOTIQ010000122         2166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_60177_length_2166_cov_6.402653, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000122 DAOTIQ010000000
VERSION     DAOTIQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60177_length_2166_cov_6.402653"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="pilM"
                     /locus_tag="P5541_09330"
     CDS             complement(<1..425)
                     /gene="pilM"
                     /locus_tag="P5541_09330"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRV72226.1"
                     /translation="MKYVEVEGKPGDLSVARSAESIVEGLAVDQEMISDTQSLESLLF
                     GIREALGGSWAPSVTIGMPSRDVLLRIVEMPPMEIEDAREALKWDFDKYFPFPYSEAT
                     FDLGVVATPGDAERENQKYIVAAARLHIVQSLLDIARRAG"
     gene            complement(577..1476)
                     /locus_tag="P5541_09335"
     CDS             complement(577..1476)
                     /locus_tag="P5541_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV72227.1"
                     /translation="MTRFAATRARKGFTLAEILIAISIGAIILLAAVSFLFTFILNLE
                     QTDEHTAATQRAEMVMTILANPVLQSGTGIPSEASDFQESLDGLTTVSGWDGPVSVVT
                     TSNPNDTVRIVYSVNTDSGLIGFSENTVSDGNTADLTFDGTSGPGSFSTDNGDPRGWC
                     VLPATGVPLRITAINGAIFTVTTAASSSKTATFVRNDSLCSLRAMEARVTGGTNPYFQ
                     TLDVGSGSGYQPRVQGISGIHFDLDSDAGILTVTVLARGNKRHDDAVTPSAPEGWNSA
                     WGFSSEDTHYRFSVLRTSWRLRN"
     gene            complement(1469..1837)
                     /locus_tag="P5541_09340"
     CDS             complement(1469..1837)
                     /locus_tag="P5541_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV72228.1"
                     /translation="MPGSHKFPSSGKQGFSLVETLIALVLLAVAVLSLALVPIATTKL
                     FVHTADHERAALLSSSLLERIESGETLDSSGTEGKFSWVITTTTEAETGEDIVRVQVS
                     WSSATGSRSSSMERLVRSDD"
     gene            complement(1917..>2166)
                     /locus_tag="P5541_09345"
     CDS             complement(1917..>2166)
                     /locus_tag="P5541_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72229.1"
                     /translation="DLAAVRSYLDRDPATSNTVATLAIVGSDVVSGDCAIEATVVDNG
                     AGARLVVMADEAGLLDADYTESTATGAVGDKFYMAVSQ"
BASE COUNT          500 a          618 c          589 g          459 t
ORIGIN      
        1 cccgctcttc tggcgatatc cagaagagac tggactatat gcagccttgc tgccgcgacg
       61 atgtatttct ggttttccct ttcggcatcg ccgggagtgg ctacaactcc aaggtcgaag
      121 gtggcctcgg aatagggaaa ggggaaatat ttgtcgaaat cccacttgag ggcctccctg
      181 gcgtcctcga tctccatggg gggcatttct acaatacgaa gcaggacatc cctcgagggc
      241 atgcctatgg taaccgaggg tgcccaggat ccccccaggg cctcccggat gccaaaaagg
      301 agggactcaa gggactgggt gtcggagatc atctcctgat ccactgccaa cccctcgaca
      361 atcgattcgg cacttctcgc aacggagagg tctcccggtt ttccctctac ctcgacatat
      421 ttgatcgatc cagttgcaag tgaaagtccc gcggcagaaa gccgtttgcc cagaagtccg
      481 atcttccatc accaccaaac cgcaggcatg ctccaccttg cgtatactga cactataccc
      541 cagcaaagga tggaactgca agggttaaat ttttttttag ttacgtaacc gccaggaagt
      601 ccgcagaaca gagaaccggt aatgcgtgtc ctcggaagag aaaccccagg ccgagttcca
      661 cccttccggc gcggaaggag taaccgcatc gtcatgtcgc ttgttgcccc tggccaggac
      721 cgtcaccgtc aggatcccgg catcgctgtc cagatcaaaa tggatcccgg agataccctg
      781 tacgcgcggc tggtaaccag acccgctccc cacgtccagg gtctggaaat atgggttggt
      841 tccccccgta actctggctt ccatcgcacg aagagagcag aggctgtcat tccggacaaa
      901 ggtcgcggtc tttgaagaag acgccgctgt agttacggta aagatcgcgc cattgatggc
      961 cgttatcctc agtgggaccc cggtggcagg aagaacacac cagcccctgg ggtcaccatt
     1021 gtccgtagaa aaggatcccg gtcccgaagt tccgtcaaag gtcaggtccg ccgtattccc
     1081 gtcggagacg gtattttcgg agaaccctat gagtccggaa tctgtgttga cggaatatac
     1141 gatcctgacc gtatcattcg ggttgctggt agtgacaacg ctcacggggc cgtcccaccc
     1201 cgaaacggtg gtaaggccgt caaggctctc ctgaaaatcg gaggcctccg aggggatccc
     1261 tgttcccgac tgcagaacgg ggttggccag tattgtcatg accatctccg cccgctgcgt
     1321 cgcggcggtg tgttcgtcgg tctgctcgag gttgaggata aaagtaaaga ggaaggagac
     1381 cgcggcgagg aggataatgg ccccgataga tatggcgata aggatctcgg cgagggtgaa
     1441 ccctttccgt gccctggtgg ccgcaaacct agtcatcgga tctcaccagc ctctccatgg
     1501 aggaacttct gctccccgtt gcggaactcc acgaaacctg gaccctgacg atatcttccc
     1561 ccgtctcggc ctcagtcgtg gtggtgatca cccaggagaa cttcccttcg gtgccgctgc
     1621 tgtccagggt ctcaccggat tcgatcctct ccagcaggga cgaggaaaga agggcggcgc
     1681 gctcatggtc ggcggtatgg acaaagagtt tcgtggtggc tatagggaca agggcaaggg
     1741 aaaggaccgc caccgcgaga agcaccagcg cgatgagtgt ctcaacaaga gaaaaaccct
     1801 gctttcctga gctgggaaat ttatgtgatc cgggcatttt actctcccct actactgtat
     1861 acatggtcct ggtttaaaaa aaggccgggc gaacccggcc ttttgattac tgttccttat
     1921 tgtgaaacag ccatgtagaa cttatctcca actgctccag tagctgttga ttcagtgtaa
     1981 tcagcgtcaa gtagaccagc ttcgtccgcc ataaccacca acctggctcc agctccattg
     2041 tcgacaacag tagcctcgat tgcacaatca ccagatacaa catcagaacc aacaatggcc
     2101 aaagtcgcaa ccgtgttgga ggtagctgga tcacggtcta gatagcttct aactgcagca
     2161 agatct
//
LOCUS       DAOTIQ010000123         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_61888_length_2121_cov_7.462730, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000123 DAOTIQ010000000
VERSION     DAOTIQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61888_length_2121_cov_7.462730"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1455)
                     /gene="zupT"
                     /locus_tag="P5541_09350"
     CDS             complement(610..1455)
                     /gene="zupT"
                     /locus_tag="P5541_09350"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRV72230.1"
                     /translation="MPDLSDVMFAFGLTLFAGLATGVGSAIAFFADRTNFRFLSISTG
                     FSAGVMLYVSFVEILLKGSESLSEVYGNVTGEWMNTAFFFLGIGVIGIIDYFIPSEEN
                     PHETRTEEETAPLHGGIPVSGVKSNSKLMRTGLFTALAIGIHNFPEGLVTFIAALQDP
                     KLGIAIAIAVALHNIPEGISVSVPIFYATGNRSKAFVYSFLSGMAEPVGALIAYGVLC
                     MMTGGGEFSPVVTGGLFAMVAGIMVYISLDELLPTSRAYGRGHDSILGLVAGMAVMAL
                     SLLLM"
     gene            complement(1448..>2121)
                     /locus_tag="P5541_09355"
     CDS             complement(1448..>2121)
                     /locus_tag="P5541_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72231.1"
                     /translation="VTVSPKLEIFPDIPPEELFSMPEGKLKWRFEENPQVLRLKELFP
                     REREPGEEGLPEVEERVDGVGAGRVLWSPHLLCVNQCDYVWYWRSRVDTDRWGLLNAD
                     RLFLRVLRDPGLFGLEEAARDMALFSLFLLDNRHVYLPGCFDIDVHQQSAFTWWFWCT
                     GTRAEAEDLASRLGREGRNLVFPAEPTDAWTISLEDLRNYDRKAESFYEFVDDLAIPV
                     TRGGA"
BASE COUNT          516 a          618 c          498 g          489 t
ORIGIN      
        1 ctgttgcccc agtgacgatc acttgtcttt cgcttcttga aagttttgcc gaaaaattcc
       61 gtgcggcgct ttcgcggcgc gaacctgcca ttcgggattt cttcgacata gactacgccg
      121 agaggaaagc agatcacgtc tcaactcaaa ccagtacttc gggagagaga tcttgaagag
      181 ttcaacctgg accgctcctt cgaagccgta agaaagatgg ccgaacttat cgccaataaa
      241 gccttttaat aaaaatgttc gccgaatctt tttcgggtaa acagactacc cccgctgacg
      301 atcatattca actgcttttc aatctttccc caagtcaact aagcctctat cttctcttta
      361 taaaaaatgt taaggcctga acctgctggg tttggctacc tattttttgg gtattcagtc
      421 cattgcgcct tgatataaac tatttgggca acaggtcgtg taggtttgac ccctctgggg
      481 ttccccttgc ccttgctttc ccaccgttta ccattgaccg ttgaccgttt acgccgtcgc
      541 tcttgccctt cacaaggaaa ggttttctct ccgttatcgg cgggcctttt tgggcgcttc
      601 tctaaaagcc tacatcaaca gcaggctcag ggccataact gccatgcccg ccacaaggcc
      661 cagaatgctg tcgtggcccc tgccataagc acggcttgtg ggaagaagtt cgtccagact
      721 tatgtacacc atgattcccg ccaccatggc aaaaagcccg ccggtgacaa cgggggagaa
      781 ctcccctcct ccggtcatca tgcacagcac cccataggcg ataagggccc ccacgggctc
      841 ggccatacca ctgaggaaag aatagacaaa ggccttcgat ctgttccccg tggcataaaa
      901 gatgggaacc gaaacgctta tgccctcggg gatgttatgg agagctaccg caatggctat
      961 ggcaataccc agcttcggat cctgcagggc agcgatgaac gtcaccagtc cctccggaaa
     1021 gttgtgtatc ccgatggcaa gggctgtaaa aaggcccgta cgcatgagtt ttgagttgct
     1081 tttcaccccg gaaacaggaa tacccccgtg aagtggagct gtttcttcct cggtccgcgt
     1141 ctcatgggga ttttcttcgg aggggataaa atagtctatg atcccgatta cccctatacc
     1201 caggaagaaa aaagccgtgt tcatccattc tcccgtcaca ttgccgtaaa cctccgaaag
     1261 agactcggag cccttcagca ggatttcgac aaacgaaacg taaagcatta ccccggcgga
     1321 gaatccagtc gagatagaga ggaaacggaa gttggtcctg tcggcgaaaa aagctatcgc
     1381 gctcccgacc ccggtggcaa gacccgcaaa aagggtcagc ccgaacgcga acattacatc
     1441 actcagatca ggcacccccc ctcgtcaccg gtatggccag gtcgtcgaca aattcgtaaa
     1501 aggattcggc ctttctgtcg tagtttctca ggtcctccag gctgatggtc caggcatcgg
     1561 tgggttcggc cggaaagaca aggtttctgc cttccctgcc cagtctcgaa gcaaggtctt
     1621 ccgcctccgc tcttgtaccg gtacaccaga accaccatgt gaaagcgctc tgctgatgga
     1681 catctatgtc aaaacagccg gggagataca cgtgtcggtt gtccaggaga aagagagaaa
     1741 agagagccat gtcccgggct gcctcttcga gtccaaacaa ccctggatct ctcaagaccc
     1801 gcagaaacaa cctgtcggca ttcagcaacc cccacctgtc ggtatcaacc cggcttctcc
     1861 agtaccagac ataatcacac tgattgacgc acagaagatg gggagaccat aaaaccctcc
     1921 ctgcgcccac cccgtcaacc cgctcttcta cctcaggcaa accttcctct cccggttccc
     1981 tttcgcgtgg gaataattct ttaagcctca ggacctgcgg attttcctcg aaacgccatt
     2041 tcaatttccc ttccggcatg gaaaacagtt cttctggagg gatatccggg aatatctcca
     2101 gttttgggga aaccgtaacc t
//
LOCUS       DAOTIQ010000124         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_63547_length_2080_cov_8.098272, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000124 DAOTIQ010000000
VERSION     DAOTIQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63547_length_2080_cov_8.098272"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1310)
                     /gene="glyA"
                     /locus_tag="P5541_09360"
     CDS             complement(15..1310)
                     /gene="glyA"
                     /locus_tag="P5541_09360"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRV72232.1"
                     /translation="MVKLTAVDPEAARLVAKELVRESSTIELIASENFVPECIMEAQG
                     SVLTNKYAEGYPGRRYHGGCEVIDEIESLAIERAKALFGAEHANVQPHSGVNANLAVY
                     MAFLDPGDTVLGMDLRHGGHLSHGSKASITGKYYRTFAYGVDEKTEQIDYDQVQKSAK
                     EHRPRLIVAGGSAYSRTIDFERFRKIADSVGAFLMVDMAHIAGLVAAGIHPSPVPWAD
                     VVTSTTTKTLRGARGGFILCREKYAAAVDKAIFPGTQGGPILQNVLAKAVTFRLASTE
                     SFREYAAQVRTNARCMAEILGSMGLRIVSGGTDNHLMLVDLRPWNQTGDRAETALGKI
                     GITVNKNMIPFDTAKPTVTSGIRIGTAAVTSRGFVEKDIEKIAAIIGEVLRQGNNYDH
                     DGYKKEVLEMCRLHPLYITPLEAGASLLSQTAANYLRKE"
     gene            1446..>2080
                     /locus_tag="P5541_09365"
     CDS             1446..>2080
                     /locus_tag="P5541_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV72233.1"
                     /translation="MAGHFDMVIKNGRVVDGTGNPSFIADVGISDGQISGIGHFDGND
                     VESFDATGLVVSPGFIDLHNHCDHTILAFPDVENYIMQGVTTSLGGNCGISMMPVNSG
                     FVELTKSYLSPFLHPGYDYGWDWKNIGGFCDKVQKNGTTQNVGFLIGHGTLRIAVKGF
                     NSSPAAPGEIDSMKKILYEGMEEGSFGLSAGLIYSPGSYATTDEMGEVASVL"
BASE COUNT          448 a          545 c          548 g          539 t
ORIGIN      
        1 cggcaatttg aagattattc cttccgaagg tagtttgctg cagtctgtga caacaacgat
       61 gctcctgcct ccaggggagt aatgtaaagc gggtgaaggc ggcacatttc caaaacctct
      121 tttttatacc cgtcgtggtc gtagttgttg ccctgcctga gaacctctcc aataatcgcc
      181 gcaatttttt cgatatcttt ttcaacgaaa ccacgggagg taacggcggc tgtgcctata
      241 cggataccgc ttgtcaccgt cggtttggcc gtgtcaaagg gaatcatgtt tttgtttacg
      301 gtgattccga tctttccgag ggccgtttcg gccctgtccc ctgtctggtt ccaggggcga
      361 aggtccacca gcatcagatg gttgtccgtt cctcctgaca ctattcgcaa acccatcgat
      421 ccaaggattt ctgccataca cctggcgttg gtacggacct gggctgcgta ttccctgaag
      481 ctttctgtag atgccagcct gaaggtcacc gccttggcca ggacgttctg aaggatggga
      541 ccaccctggg ttcccgggaa gatggccttg tcaaccgcag cggcgtattt ttcccggcac
      601 aggataaagc ctccccgggc gcccctcagg gtttttgttg tggtactggt gacgacatcg
      661 gcccatggca cggggctggg gtggatgccg gcagcgacaa ggccggcaat atgtgccata
      721 tccaccatca ggaaggctcc gacgctgtcc gcgatcttcc tgaacctctc gaaatcgatc
      781 gtccttgaat acgcgcttcc tccggcgacg ataagtcttg gcctgtgttc tttcgcggat
      841 ttctggacct ggtcgtaatc gatctgttcg gtcttttcat caaccccgta ggcaaaggtc
      901 ctgtagtact tcccggttat gcttgccttg ctgccgtgcg agagatgtcc tccgtggcgg
      961 aggtccatac ccaggacggt gtcccccgga tcaagaaacg ccatatagac ggcaaggttt
     1021 gcgttaacac ccgaatgggg ttgaacgttc gcatgttctg cgccaaagag cgcctttgcc
     1081 ctttcgatag caaggctttc gatctcgtcg atcacctcac agcctccgtg gtatctccgc
     1141 cccggatagc cttccgcata tttgttggta aggaccgatc cctgggcttc cataatgcac
     1201 tccgggacga aattctccga agctatcagt tcgatggtgg aactttcgcg aaccagttct
     1261 tttgcgacaa gtcttgccgc ctcgggatcg accgctgtca gctttaccac cctgagcaca
     1321 acctttctct gttaaggttc tggatatttg gtgaaaagcc aggaccatac cattttacag
     1381 gtcaattttc tttactccat ggtagagttt gtccagatga acgaaaaagg agagatcaac
     1441 acaaaatggc cgggcatttc gacatggtta tcaaaaacgg gcgagtagtc gacggtaccg
     1501 ggaatccgtc cttcattgcg gatgtaggta tcagtgacgg gcagatatca gggataggtc
     1561 actttgatgg gaatgatgtc gaatcttttg atgccacggg gcttgtcgtt tccccgggtt
     1621 tcattgatct tcacaatcat tgcgaccata caatacttgc cttccccgat gtggagaact
     1681 acataatgca gggagtcaca acttccctgg gaggcaattg cggtatttcg atgatgccgg
     1741 tcaactccgg gttcgtggaa ttgacgaaaa gttatctttc tccattcctg catcccggtt
     1801 acgactatgg ctgggactgg aaaaacattg gtggtttttg cgacaaggtg caaaaaaacg
     1861 gcacgaccca gaacgtgggt ttcctcatcg gtcatggaac cctcaggatc gcggtcaagg
     1921 gcttcaatag cagccccgca gcacccggag aaattgactc catgaaaaag atcctttatg
     1981 aggggatgga agaaggttct tttggtcttt cagctggact catatattct cccgggagtt
     2041 atgcaacaac cgacgagatg ggcgaagtgg ccagtgtcct
//
LOCUS       DAOTIQ010000125         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_69334_length_1951_cov_6.597046, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000125 DAOTIQ010000000
VERSION     DAOTIQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69334_length_1951_cov_6.597046"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="P5541_09370"
     CDS             complement(<1..268)
                     /locus_tag="P5541_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRV72234.1"
                     /translation="MQEKNHETRKIYNSRLGATVVKALEANGFKARYAASFEDAVKAV
                     LELVPEGSSVGVPGSSTIRELGLFETLESRGCKIFHHWDPSLAPA"
     gene            434..712
                     /locus_tag="P5541_09375"
     CDS             434..712
                     /locus_tag="P5541_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72235.1"
                     /translation="MCRVITPDQGGIHSHVDDPLSIAELLRETLVEELQAMTELSARW
                     HLIEDEEVRHTLLHLIEDKRKSISVLWDVMRKTEDRALGEGKHHGHSH"
     gene            811..1185
                     /locus_tag="P5541_09380"
     CDS             811..1185
                     /locus_tag="P5541_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRV72236.1"
                     /translation="MPVLEDQIEMRVHLSTLFDIYDPVLTRKQSEAFRLHFLNDLSLS
                     EVADRLEVTRQGAHDLVQRGKERLLEMESLLGFARKESEWNIRLQEIRSWSDDFGGDI
                     PQEALTSLEEIIGTEIHGREAI"
     gene            1185..>1951
                     /locus_tag="P5541_09385"
     CDS             1185..>1951
                     /locus_tag="P5541_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRV72237.1"
                     /translation="MFDSLRERLESVFKGLKNRGKLTEEDVSAALRDVRRALLEGDVN
                     YRVVKDLVEKIKGRAVGREVLDSITPAQQVIAIVYEEISALMGSGKSSLSFSSRPPTK
                     ILIAGLQGSGKTTTCVKIAKKISDSHKPLVVACDLRRPAAVEQLRVLAERAGTGFFGP
                     EEGESDVCILAGKAMEFAALRLYDVIIFDTAGRLDIDDELMEELSSLNRKVEPTETLL
                     VVDSMTGQEAVNVASAFHEKVPLSGLVLTKVDGDSRGG"
BASE COUNT          490 a          472 c          538 g          451 t
ORIGIN      
        1 gtgctggagc cagtgacggg tcccagtgat ggaatatctt gcaccccctc gactccagtg
       61 tctcgaacag acccagttcc cgaatagttg aggaaccagg aacccccaca ctggagccct
      121 cgggcaccag ttccagcaca gcctttacgg cgtcctcaaa tgaagccgcg taacgggcct
      181 tgaatccgtt ggcctccagc gcttttacca cagtagctcc caggcggctg ttgtagattt
      241 ttcttgtttc gtggtttttt tcctgcatgg ggttcaattt caccgactcc ttccctgggg
      301 gaaatttact ttttctattc taacagaata gcatgcaaag tgtcagaagg ctcccgtagg
      361 acctggtgat tgcgatgaaa tatgataaaa taccgaatca gtaacatcga agcgaaagat
      421 cggagggatt cgaatgtgca gggtaattac gccggaccag gggggaatcc acagccatgt
      481 cgatgacccg ctttccatag ccgaacttct gagggaaacc cttgttgaag aactgcaggc
      541 tatgacggaa ctgtcagccc gatggcacct gatcgaggat gaagaagtga gacataccct
      601 cttgcatctg atcgaagaca agagaaaatc catttcagtc ctctgggatg taatgagaaa
      661 aacggaggat cgagcccttg gagagggcaa acatcacgga cattcccact aattcatgat
      721 ccctggatcc tgaggccccc ggaactgtca agtaatatta cttgacagtt ccgggggctt
      781 tccatataat cgatccttgc ggatatgggg gtgcctgttc tggaagacca gatagaaatg
      841 cgtgtccatc tttcaactct tttcgatatc tatgatcccg ttctgacccg caagcaatcc
      901 gaggccttca ggcttcattt cctcaatgac ctttctcttt cggaggttgc tgacaggctt
      961 gaggttacca gacagggagc ccatgacctt gttcagagag gaaaggaaag gctgcttgag
     1021 atggaatcgc ttctgggttt tgccagaaaa gagagcgaat ggaatatacg cctccaggaa
     1081 ataagatcct ggtccgacga ctttggcgga gatatccctc aggaagcact gacctctctg
     1141 gaagaaatta tcggtacaga aatccacgga agggaggcca tctgatgttt gattctctaa
     1201 gggaaagact cgagagtgtg ttcaagggtc tgaaaaacag gggaaaactt accgaagagg
     1261 atgtatctgc tgctctgcgc gatgtcagac gggctctcct tgaaggggac gtgaactaca
     1321 gggttgtgaa ggacctggtt gagaagataa agggccgggc tgtcggccgc gaggttcttg
     1381 attccattac tccagcccag caggtaattg ccatagtgta cgaagaaata tcggcactga
     1441 tgggaagtgg gaaaagctcc ctttctttct cctccaggcc tccgacgaag atcctgatcg
     1501 cgggtctcca gggctccgga aagacaacca cctgtgtgaa gatagccaaa aagatatcag
     1561 attcccacaa gccactggta gtggcctgtg acctgagaag acctgccgcg gtggagcagt
     1621 tgcgcgtcct tgccgaaagg gccgggacgg gttttttcgg cccagaagaa ggagaaagcg
     1681 atgtctgcat tctggccggc aaggcgatgg aatttgccgc cctgagactt tacgatgtca
     1741 tcatcttcga tacggcggga agattggata tagacgacga actcatggag gaactctcct
     1801 cgttaaacag gaaggtcgag cccaccgaga cccttctcgt ggtggattcc atgacgggtc
     1861 aggaggcggt caatgtcgct tccgcatttc atgagaaggt gcctctcagc ggtctggtgc
     1921 tcaccaaggt tgacggtgat tcaagaggtg g
//
LOCUS       DAOTIQ010000126         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_75601_length_1832_cov_4.776027, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000126 DAOTIQ010000000
VERSION     DAOTIQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75601_length_1832_cov_4.776027"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5541_09390"
     CDS             complement(<1..351)
                     /locus_tag="P5541_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRV72238.1"
                     /translation="MDERLIRIDYGTAFPGLDPESEIGSLKELLLLSSARIIEGSREG
                     EEGFGWFDLPEADTAPVRKHAEWLRSFDTVIQTGIGGSALGNQMLSGALTHPYYNELA
                     SEERGGPRSYVSDNA"
     gene            complement(489..728)
                     /locus_tag="P5541_09395"
     CDS             complement(489..728)
                     /locus_tag="P5541_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRV72239.1"
                     /translation="MAKVRVYSTPTCPWCVKMKDYLKSRNVDFENVDVSADREAAMEM
                     VKKTGQMGVPVALVGDVYIVGFDKAKIDEELAKLD"
     gene            1009..1365
                     /locus_tag="P5541_09400"
     CDS             1009..1365
                     /locus_tag="P5541_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRV72240.1"
                     /translation="MRLGDIMNLLEAELLNQRVDLDIELESAFAADLMSDVLALARPG
                     SLMITGLTNIQIMRTASMIDMPAVVFVRGKKPPLETIELAGKLGIPFMITYKTMFETC
                     GLLYEAGIKPIRCEKR"
     gene            1371..1817
                     /locus_tag="P5541_09405"
     CDS             1371..1817
                     /locus_tag="P5541_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV72241.1"
                     /translation="MRPGKTILVQREVKPLDFLSAGETASEFKETLKSLGISADLCRK
                     TSIVAYEAEMNAVIHGGGGEIILKLDEKSITIVAKDQGPGIENVSLAMQEGYSTAPDF
                     IREMGFGAGMGLPNIKKYSNELDIETEPGKGTTLRALIYLDGSGGE"
BASE COUNT          468 a          473 c          437 g          454 t
ORIGIN      
        1 ggcgttgtcg gaaacataag acctgggtcc tccccgttcc tccgatgcaa gttcgttgta
       61 ataagggtgg gtcagtgcac cagaaagcat ctggttcccc agagccgagc ctcctattcc
      121 tgtctggatc accgtatcaa aggaacgtag ccattcggca tgtttcctta cgggggctgt
      181 atcggcctca ggaagatcga accagccaaa accctcttct ccctctctgg atccttcgat
      241 gatcctggcc gaggataaca gcaacaattc cttcaaagat cctatttcgc tttcggggtc
      301 cagaccgggg aaagcggttc cgtaatcgat ccggatcagt ctttcatcca caggaacacc
      361 gcccttcaga aaaaccttaa atttattgac aggtttacct gattgtaacc acacacaagg
      421 acaaaaaaaa ggcggacccg caatttctca gcgggtccgc cggtcttctt tccggttact
      481 ggccagtctc agtcaagttt tgcaagctcc tcgtctattt tggccttgtc aaaaccaaca
      541 atgtaaacat ctcctacaag agccaccgga actcccatct ggcctgtctt tttgaccatt
      601 tccatcgcgg cttccctgtc cgcgctgacg tccacgttct caaaatcgac gttcctggat
      661 ttgagataat ctttcatttt tacacaccat ggacacgtcg gtgtcgagta aaccctgact
      721 ttcgccacaa cctttcacct ccatgatttc tcttattcgt gcttttgtat gaaacatata
      781 cccttcggtg gtaattctac tcttccattc gtgactgtca agatagtctt tctgcttatt
      841 gatccgaata aatctgttta ttagattata ctgtataatc gtccccgaat gaaaacggga
      901 gatcatgcac gtctgtttca tttttctgtc tggaatttga gaaaattggc ccaaaacatg
      961 atatagttat ctcaatcact ttcttgtccg gttcggggag ggaaatttat gcgtctgggc
     1021 gatatcatga atctgcttga ggccgaactt ctcaaccaga gggtagatct tgacattgaa
     1081 cttgaatcag catttgcggc agatctgatg agcgatgttc tggctcttgc ccgacccggt
     1141 tctcttatga taacgggact tacaaacata cagataatga gaaccgcaag catgatagac
     1201 atgccggcag tcgtttttgt cagggggaag aaacctcccc tcgaaaccat agaactggcg
     1261 ggcaaactgg ggatcccgtt catgatcacc tacaagacca tgttcgagac ctgcggcctg
     1321 ctctacgagg cggggatcaa accgatccgc tgcgaaaaac ggtgaacccg atgagacctg
     1381 gcaagacgat actggtccag cgggaggtta aacccctgga ctttctttct gcgggggaga
     1441 ctgcctcgga gttcaaggag accctgaaat ctctggggat ttcagccgac ctctgcagga
     1501 aaacctccat agtggcgtac gaggcagaga tgaacgccgt catccatgga ggcgggggag
     1561 agatcattct caaactcgac gaaaagagca taaccattgt cgcgaaagac caggggcccg
     1621 gcatagagaa cgtaagcctg gcaatgcagg aaggctactc cacggcaccc gacttcattc
     1681 gcgagatggg cttcggagcc ggaatggggc ttccgaacat aaaaaaatat tccaatgaac
     1741 tggacatcga gactgaaccc ggaaaaggga caacactcag ggctttgata tatctcgatg
     1801 gcagcggggg tgaataggag ttgactcacg gc
//
LOCUS       DAOTIQ010000127         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermovirgaceae bacterium isolate AMR_MDS_3021
            NODE_82602_length_1721_cov_8.618247, whole genome shotgun sequence.
ACCESSION   DAOTIQ010000127 DAOTIQ010000000
VERSION     DAOTIQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868045
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermovirgaceae bacterium (wastewater metagenome)
  ORGANISM  Thermovirgaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Thermovirgaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:16:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,885
            CDSs (total)                      :: 1,861
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 24
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thermovirgaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82602_length_1721_cov_8.618247"
                     /isolate="AMR_MDS_3021"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3041276"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..398
                     /locus_tag="P5541_09410"
     CDS             87..398
                     /locus_tag="P5541_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72242.1"
                     /translation="MLKLEVEHYLELAEKVRERATELCCLYLKVHFDCENCSVFVTNV
                     ETGKSGRFRIMFTESDMEDILNLPAEYFWLTDDEVSVRMTIDRDIREGELEERRKSRA
                     R"
     gene            complement(416..793)
                     /locus_tag="P5541_09415"
     CDS             complement(416..793)
                     /locus_tag="P5541_09415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV72243.1"
                     /translation="MADIDHFKKVNDTHGHHAGDVVLASFGKILSGQTRGSDMAGRYG
                     GEEFLVVMPRTGLEEAEKIAERIRAEVERAIMLDPGIRVTASFGVAERKSCKNWESLV
                     KRADEALYLAKDTGRNKVVVSKK"
     gene            complement(735..1121)
                     /locus_tag="P5541_09420"
     CDS             complement(735..1121)
                     /locus_tag="P5541_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72244.1"
                     /translation="MSVSLVQFIEKLPAIPGDAAKEFADSRGSIIARVNSAMEAREDL
                     TILTGGNPVSVVRDNHVNHANTMAVTFGLGSLDILAMTAPWVYRSYIYHGFSPDYFPV
                     LFSVFRDNGGYRSFQEGERHTRSPRR"
     gene            complement(1154..1654)
                     /locus_tag="P5541_09425"
     CDS             complement(1154..1654)
                     /locus_tag="P5541_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV72245.1"
                     /translation="MKKFLVVTCILVLPVVGLFYGSSWASPSDTFSSDSIDSLGRSES
                     EMRQIHGEPLSTIISDIKNPHWPDVIDKKVEYRYEDVSFLFYRPSQTPGTAILMKIEV
                     SDPELKFSGLGVGSVRKEVQALLGCGTPWENDLIFEDADGFDSVNIRFDNKEKITKFI
                     FIPYSD"
BASE COUNT          441 a          444 c          407 g          429 t
ORIGIN      
        1 ccaggattaa aattcagggt agcattttac cgtgacgcta tgatatactc cctcaggatg
       61 gttttcctga aggaggcgtg ggcaagttgc ttaaactcga ggtcgaacac tacctggaac
      121 tcgcggaaaa ggtcagagaa agggctaccg aactctgctg cttatatctc aaggtccatt
      181 tcgactgcga gaactgcagc gtatttgtta cgaacgtgga gaccggcaaa agcggaaggt
      241 tccgcataat gttcaccgag tcagatatgg aagatattct caacctcccc gcagaatatt
      301 tctggcttac tgacgacgag gtctccgtaa gaatgaccat agacagggac ataagggaag
      361 gcgaactcga ggaacggaga aaatcacggg cccgataatg ggaaaatctg ttcagttact
      421 tttttgatac cacgaccttg ttcctgccgg tatcctttgc aagatacagg gcctcgtcag
      481 ctcttttgac gaggctctcc caattcttgc aactctttct ctcggccaca ccaaaactgg
      541 cagtcactct aatcccggga tcaagcatta ttgccctttc aacttccgca cggatccttt
      601 cggcaatttt ctccgcctct tcaagaccgg tcctcggcat tactacaaga aactcttctc
      661 ctccgtaacg tccggccata tcgctgcctc ttgtctgccc tgaaaggatc ttcccgaagg
      721 aggccagcac gacatcaccg gcgtggtgac cgtgtgtgtc gttcaccttc ttgaaatgat
      781 cgatatccgc cattatccct gaaaacactg aaaaggaccg ggaagtaatc aggagagaaa
      841 ccatggtaga tgtaggagcg atagacccag ggagccgtca tggccaggat gtccagggaa
      901 cccaggccga aggtcacggc catagtgtta gcgtggttga cgtggttatc tctgacaacg
      961 ctcaccggat tccctccggt gagtatcgta aggtcctccc tggcctccat ggccgaatta
     1021 acccttgcta ttatcgaccc tcttgaatcc gcaaactctt ttgcagcatc ccctggtatt
     1081 gccggcagtt tttcgatgaa ttgaacaagc gaaaccgaca taccgcaccc cccttgagta
     1141 caggactttt ggatcagtca gagtacggaa tgaagataaa ctttgtgatt ttttccttgt
     1201 tgtcgaagcg gatatttacg ctatcgaatc cgtccgcatc ctcgaaaata agatcattct
     1261 cccatggagt tccgcaaccc agcagcgcct ggacttcttt cctgacggat ccaactccga
     1321 gcccgctgaa tttcaactcg ggatcactta cctctatttt catgaggata gccgtgccgg
     1381 gagtctggga gggacggtaa aagaggaagg aaacatcttc gtaccgatat tcgaccttct
     1441 tgtcgataac atctggccag tgaggatttt tgatatcaga gatgatggtt gagagaggtt
     1501 caccatggat ctgcctcatt tctgattcgc ttctgcccag gctgtctatt gaatcggaac
     1561 tgaaagtatc cgaaggggaa gcccacgaag atccgtaaaa caatcccacc accggaagaa
     1621 cgagaataca ggtcactacc aggaacttct tcatcggaga atctcctttc ctttgcggag
     1681 catttgttta ataatatcat ggacgaaggt gtcacataac a
//
