LOCUS       DAOTJA010000001        31139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_1728_length_31139_cov_6.050154, whole genome shotgun sequence.
ACCESSION   DAOTJA010000001 DAOTJA010000000
VERSION     DAOTJA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 31139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31139
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_31139_cov_6.050154"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1194)
                     /locus_tag="P5241_00005"
     CDS             complement(277..1194)
                     /locus_tag="P5241_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV31995.1"
                     /translation="MYDLIILGTGPAGMTSSIYAQRYNLNYLLVGDVLGGMMIYPENI
                     TNFPSYTSISGIDLTNNFKQHLNSLGIKPIESEAVRILQKEKYFEVFLKNEQQYETRN
                     LLIALGTSKRKLNIKGEDKFMGKGVHYCATCDAVFYVNKIVGVVGGSDSATSAAELLA
                     EKSKHVYLIVRSNEIKGSQERISNLRNRNNVTIILENEISEIIGDKFVNKIILKNEFN
                     GTKELAIDGIFVEIGGVPNISLFRNLNIGLDDKEYIIVDKNQKTTVDCVYAAGDITNN
                     HLGFKQIVTACAEGAIAVYNIYQNLKLKV"
     gene            complement(1293..1363)
                     /locus_tag="P5241_00010"
     tRNA            complement(1293..1363)
                     /locus_tag="P5241_00010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1329..1331),aa:Thr,seq:cgt)
     gene            complement(1377..2138)
                     /gene="map"
                     /locus_tag="P5241_00015"
     CDS             complement(1377..2138)
                     /gene="map"
                     /locus_tag="P5241_00015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRV31996.1"
                     /translation="MITIKTEKEIKILKQGGHILAKILKTVANSIYPGISTDILDKMA
                     FDLIKQAGGKPSFLHYKADFMSKEYPASLCVSKNNIIVHGIPSNKTFLNQGDIVSLDI
                     GMVYKGLYTDTAITVPVGDICLKYKKLIKATKESLYKAILVTKAGNTLGDIGYTIQST
                     LTKYGLLPIKNLVGHGVGYQAHEDPEIFNFGMPHSGVVLKAGYVLAIEPMATYGTDDI
                     IENPDGSFSTANGEYAAHFEHTIAILKTGNIIITK"
     gene            complement(2251..2484)
                     /locus_tag="P5241_00020"
     CDS             complement(2251..2484)
                     /locus_tag="P5241_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV31997.1"
                     /translation="MAFTPDITKCPDDQSILTIRNDDKPEVVENRIKVFHNEIMPTIN
                     FFQDHGMLHTIDGIGSLEEVFQRIDQVIKNFLS"
     gene            complement(2590..2883)
                     /locus_tag="P5241_00025"
     CDS             complement(2590..2883)
                     /locus_tag="P5241_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRV31998.1"
                     /translation="MGKPNSGKGTQALKIVEKYKMVHINVGEELRSWVSQDQRFIAQK
                     VEQDLNSGAAVPICIAIKAWMNKLISLSADDSILFEGSPRKLLEAEAMLEYFD"
     gene            complement(3063..4139)
                     /gene="secY"
                     /locus_tag="P5241_00030"
                     /pseudo
     CDS             complement(3063..4139)
                     /gene="secY"
                     /locus_tag="P5241_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
     gene            complement(4359..4796)
                     /locus_tag="P5241_00035"
     CDS             complement(4359..4796)
                     /locus_tag="P5241_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HRV31999.1"
                     /translation="MQINNRKLQSKRKRAKRVGRGGKRGTYSGRGQKGQKSRAGRKIH
                     SELRVMFLRIPKRRGSGNKTIPKRTKNIGLDTIAKNFNDNTIISPKSLKEKGLIKSVK
                     DNVKILGSKNVLSKKYTIIDINMSQGAKNQLEKAGGIIKSTKK"
     gene            complement(4765..5439)
                     /locus_tag="P5241_00040"
     CDS             complement(4765..5439)
                     /locus_tag="P5241_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32000.1"
                     /translation="MLKNNPNNLKTNTKTSNPLPPKENSSNFRSTSKFVSRKPRSSNE
                     DFDQIILEIKRVTRVTGGGKKLSFRAAILLGNHRDKIGFGIAKGSDVPQAIEKAKKEA
                     MKNIITIPIKNDTIPFAVSGKYCASKVLLKPAKAGKGIIAGGVLRDIIKMTNIKNITT
                     KILGGSHNVVNNTRAFLLAISKLNTNQNKENKINNEKGDTQNKNNFKENDNIEQYADK
                     QSEVTI"
     gene            complement(5442..5795)
                     /gene="rplR"
                     /locus_tag="P5241_00045"
     CDS             complement(5442..5795)
                     /gene="rplR"
                     /locus_tag="P5241_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014918232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRV32001.1"
                     /translation="MDIAKKRKIQRKHIHKRIRSKISGTSDKPRISVFRSNKHMYVQV
                     IDDEKQITLLSISDHDIKDIKKKTDIASMLGTMLSEKMKAKNITKAVFDRGGYQYHGR
                     VQALADSLRKNGIIF"
     gene            complement(5823..6158)
                     /gene="rplF"
                     /locus_tag="P5241_00050"
     CDS             complement(5823..6158)
                     /gene="rplF"
                     /locus_tag="P5241_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRV32002.1"
                     /translation="MIQNNIIGVTEGFEKKLVIEGIGYKADVNGDKLVLNIGYSHPVF
                     VDIPKGIEVSVEKNVISIKGISKQNVGDFAANVRKLRKTNPYSGKGIHYQGEHIIRKT
                     GKKVASSGS"
     gene            complement(6174..6371)
                     /gene="rplF"
                     /locus_tag="P5241_00055"
     CDS             complement(6174..6371)
                     /gene="rplF"
                     /locus_tag="P5241_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRV32003.1"
                     /translation="MSKIGKKILTIPDDVELIVNNKEVTIKGPKGMLTSILPEGFNIV
                     QNKNKLSIIPPSKIDHLKSAL"
     gene            complement(6412..6801)
                     /gene="rpsH"
                     /locus_tag="P5241_00060"
     CDS             complement(6412..6801)
                     /gene="rpsH"
                     /locus_tag="P5241_00060"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRV32004.1"
                     /translation="MSDPIADMIIRIKNASAINKPMVNIPVSKLKKEILLTLQKENVI
                     ENFTEIGKGVHKNFEVIIKYNNNIPAIKDVKRISKPSCRVYIKAKDIHPLKKGFGLRL
                     LSTPHGIMSDREAKQLNLGGEVLFEIY"
     gene            complement(6847..7029)
                     /locus_tag="P5241_00065"
     CDS             complement(6847..7029)
                     /locus_tag="P5241_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRV32005.1"
                     /translation="MSKKSTYARSKKTPKYSTRKVLRCFRCGRKRGYMRDFGLCRICF
                     REMASRGEIPGVKKSS"
     gene            complement(7054..7581)
                     /gene="rplE"
                     /locus_tag="P5241_00070"
                     /pseudo
     CDS             complement(7054..7581)
                     /gene="rplE"
                     /locus_tag="P5241_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012877.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(7590..7913)
                     /gene="rplX"
                     /locus_tag="P5241_00075"
     CDS             complement(7590..7913)
                     /gene="rplX"
                     /locus_tag="P5241_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRV32006.1"
                     /translation="MKLKKGDKVKIIKGKDAGKTGTISRTFPKNNTILIEGFNTYKKN
                     RKPRKEGEKGQIVVLSRPFCSSKAMLICPKCGKPTRIGFIIETDKNNKKVKIRICHKC
                     KAKLN"
     gene            complement(7985..8353)
                     /gene="rplN"
                     /locus_tag="P5241_00080"
     CDS             complement(7985..8353)
                     /gene="rplN"
                     /locus_tag="P5241_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRV32007.1"
                     /translation="MIQLRSLLKVADNSGAQIVRCFQIRGGSYHRYGQIGDVIMAAVQ
                     VAKPRKSVKKKDIVRCVIVRQRKNYRRANGSYIRFDDNAVVLINDKKEPIGTRIFGPI
                     PREIKDKGFNKIIELGPELV"
     gene            complement(8665..8874)
                     /gene="rpsQ"
                     /locus_tag="P5241_00085"
     CDS             complement(8665..8874)
                     /gene="rpsQ"
                     /locus_tag="P5241_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRV32008.1"
                     /translation="MPRQLKGIVVSDKMTQTCVVKVDIFQKHPRYKKFFVLSKKVKVD
                     NPNSIYKTGDKVIIEETRPISKTKC"
     gene            complement(8867..9067)
                     /gene="rpmC"
                     /locus_tag="P5241_00090"
     CDS             complement(8867..9067)
                     /gene="rpmC"
                     /locus_tag="P5241_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRV32009.1"
                     /translation="MKIKDLEKKDLNELQKLLTEKQKQAIQAKFDLKMGKVKNVKILF
                     NLKKDIAQISTIINQLLSQKHA"
     gene            complement(9091..9525)
                     /gene="rplP"
                     /locus_tag="P5241_00095"
                     /pseudo
     CDS             complement(9091..9525)
                     /gene="rplP"
                     /locus_tag="P5241_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767121.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
     gene            complement(9746..10123)
                     /locus_tag="P5241_00100"
     CDS             complement(9746..10123)
                     /locus_tag="P5241_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRV32010.1"
                     /translation="MRKCLEKLLSKAHYSEIIIERKSDKINITIYTSRPGMIIKHQGE
                     GLGELKKAIEKTLEKYYRKNKIDKNINISLNIEEVRKPFLSAQIVAFDVATEFEKRQP
                     YRKTIKKYVEKIMQNKEAKGAKI"
     gene            complement(10246..10761)
                     /gene="rplV"
                     /locus_tag="P5241_00105"
     CDS             complement(10246..10761)
                     /gene="rplV"
                     /locus_tag="P5241_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRV32011.1"
                     /translation="MEITASLKYLKIAPRKVRLVANIIKGLPISSAEDQLRHISNRSA
                     APILKLLMSAKANAVNNFHLDPSTLFIKSIVVNQGPMLKRALPRAHGATNVIRHRMSH
                     IVITLDTAHSLKSLNKIAQSPEIAKTQESKTVYEKNKPRNKNTKFYRETKQTKAKKGQ
                     VKPIIQRKVIK"
     gene            complement(10790..11110)
                     /gene="rpsS"
                     /locus_tag="P5241_00110"
                     /pseudo
     CDS             complement(10790..11110)
                     /gene="rpsS"
                     /locus_tag="P5241_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559433.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(11177..12022)
                     /gene="rplB"
                     /locus_tag="P5241_00115"
                     /pseudo
     CDS             complement(11177..12022)
                     /gene="rplB"
                     /locus_tag="P5241_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499639.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            complement(12058..12435)
                     /locus_tag="P5241_00120"
     CDS             complement(12058..12435)
                     /locus_tag="P5241_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRV32012.1"
                     /translation="MSSILSRFKTNKKISTKTNKTNISKPNTAVKKDWNFVAFFCDPY
                     ITEKATLDKEGNKYHFLVSKKANKIEIKKFIEKFYNVKVADVNISKKFFSPVNFRNHI
                     SHKAPIKKAIVTLKPGQKIEFNI"
     gene            complement(12450..13103)
                     /gene="rplD"
                     /locus_tag="P5241_00125"
                     /pseudo
     CDS             complement(12450..13103)
                     /gene="rplD"
                     /locus_tag="P5241_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            complement(13125..13265)
                     /locus_tag="P5241_00130"
     CDS             complement(13125..13265)
                     /locus_tag="P5241_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32013.1"
                     /translation="MAGRMGGDTVTIKNLTILDIDVDNNLIFLKGAVPGVKGSLVKIE
                     SK"
     gene            complement(13368..13688)
                     /locus_tag="P5241_00135"
     CDS             complement(13368..13688)
                     /locus_tag="P5241_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32014.1"
                     /translation="MKFLLAKKLTMTRLPDPSEGFVAVTVLQVIPNSVSQIKTNKTDG
                     YNAVQISVDKNKKKKSKKEFRVDNPEEYKIGDIIDIKQFAPGDIVKVQGISKGKGFQG
                     VVKR"
     gene            complement(13817..14155)
                     /gene="rpsJ"
                     /locus_tag="P5241_00140"
     CDS             complement(13817..14155)
                     /gene="rpsJ"
                     /locus_tag="P5241_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRV32015.1"
                     /translation="MSSSTLENKDKDKIKIRVRLKSYDHRIIDNCCKQIIDIVVRNGA
                     QVIGPILLPTEIKRYSVNRASFVKKTSQEQFEIRVHKRIIDILNPNQKIIESLTNLNL
                     PAGVEIEIKT"
     gene            complement(14180..15379)
                     /gene="tuf"
                     /locus_tag="P5241_00145"
     CDS             complement(14180..15379)
                     /gene="tuf"
                     /locus_tag="P5241_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRV32016.1"
                     /translation="MAEKEKFVRTKSHLNVGTIGHIDHGKTTLTAAILNTLHLKGTVA
                     KAVSVNDIDKAPEEKSRGITINVHHSEYETDKRHYAHIDCPGHVDYIKNMITGSAQMD
                     GAILVVSAADGPMPQTREHVLLARQVGVPAIVVFLNKCDAVSDPEMIDLVETEVRELL
                     NKYGFPGNDTPIIRGSALKALEATSYDDDAVKPIIQLLDTLDSYIPEPVRDKDKPFLM
                     PIEDIFTIEGRGTVVTGRIERGTIHINDEVEIVGYTPTIKTVVTGVEMFNKTLDEGLA
                     GDNVGLLLRGTKKEDVKRGQVTAKPGSITPHTEFESEVYVLSKDEGGRHTPFFTGYKP
                     QFFIRTADITGEVTLKEGVEMVMPGDTATFKVKLIGPIALEEKQKFAIREGGKTVGAG
                     VITKIIA"
     gene            complement(15505..17604)
                     /gene="fusA"
                     /locus_tag="P5241_00150"
                     /pseudo
     CDS             complement(15505..17604)
                     /gene="fusA"
                     /locus_tag="P5241_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(17690..18160)
                     /gene="rpsG"
                     /locus_tag="P5241_00155"
                     /pseudo
     CDS             complement(17690..18160)
                     /gene="rpsG"
                     /locus_tag="P5241_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038110.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
     gene            complement(18193..18603)
                     /gene="rpsL"
                     /locus_tag="P5241_00160"
     CDS             complement(18193..18603)
                     /gene="rpsL"
                     /locus_tag="P5241_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRV32017.1"
                     /translation="MPTVNQLRKKGRSPIIKKSKTRGLGFAFNIKKNRIKNYSSPFKK
                     GVCTKVFTMTPKKPNSALRKVARVKLSNGLEVTAYIPGIGHNLQEHSIVLIRGGRVKD
                     LPGVRYHIIRGKYDTAGVDNRKQQRSKYGAKRSK"
     gene            complement(18919..19131)
                     /locus_tag="P5241_00165"
                     /pseudo
     CDS             complement(18919..19131)
                     /locus_tag="P5241_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553121.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
     gene            complement(19381..20301)
                     /locus_tag="P5241_00170"
                     /pseudo
     CDS             complement(19381..20301)
                     /locus_tag="P5241_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            complement(20548..21039)
                     /locus_tag="P5241_00175"
     CDS             complement(20548..21039)
                     /locus_tag="P5241_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV32018.1"
                     /translation="MSKQNKDQKPVNIPKPTIDDIHNDVSGYTAKDIYVLEGLEPVRR
                     RPGMYIGSTGLSGLHHLIFEVTDNSLDEVAAGFCKNIEISLLPNNRVSVLDDGRGIPV
                     DIHKQTKKSALETVLTTLHAGAKFGGKSYKIAGGLHGVGVSVVNALSKYLKAEVYRDN
                     FVY"
     gene            complement(21108..21680)
                     /locus_tag="P5241_00180"
     CDS             complement(21108..21680)
                     /locus_tag="P5241_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32019.1"
                     /translation="MLKAKGILESLFSKFGISEHSYFTSNKLPNLLDKLYGIFADNNS
                     NIIFYTNDNKYLASLIIPSNKILDIYDITIEYKKPIVILGEINLQELFKLVKQEIEFN
                     PLPKYPSSIKDLSILVPQNTLIQDIYLAIQQSNANNLADIDIFDIYEGLDKGKSISFH
                     LMFRSDDHTLTDQEIKNEFDKIVKNLKQKG"
     gene            complement(21681..23228)
                     /locus_tag="P5241_00185"
     CDS             complement(21681..23228)
                     /locus_tag="P5241_00185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRV32020.1"
                     /translation="MQYSYNWLKEYIPNKLPEAKKLAEILNYFIGETTYKKHGSDFVL
                     NIDIPVNRFADLGGHLGLAREIATCLDLNLNQKLIKIPQIKFVNAKNSYLNIQVDDPN
                     DCPQYCGLLIQNVEVKPSPKEIQSKLLSCGLRSINNIVDITNLVMLEYNQPMHAFDYD
                     RINIDQTADKRELNSINKHHSNSALIKIRRARAGEKITTLDGTPIELNENILVVANQY
                     KPLAIAGIKGGEYAGITKNTKNIVLESANFNSSLIYKSSKYLNLSTDASCRFAHNLPQ
                     ELTYVSLLKAAFLIKKYANGTIIEPAIRTNSKNNKPTIILLNLEKIYRFLGFNVDVKF
                     LENILHKLEFQFVKRNPKLYLVSIPYWRLDLQNEDDLIGEIGRIFGFNNIPFIAPQIT
                     LSYPKDNEFYNFKQYLKTYSLGLGLSEIHEYNFIGAKEINILDKSILPNLVKIKNPQS
                     SEYLYLRPFSLVSSLRAVSTNIKTFPTAQFFEISKNFLNKNNTVVESDCYSFAIYNSQ
                     NSVRVSD"
     gene            complement(23365..24150)
                     /gene="pheS"
                     /locus_tag="P5241_00190"
                     /pseudo
     CDS             complement(23365..24150)
                     /gene="pheS"
                     /locus_tag="P5241_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
     gene            complement(24160..24429)
                     /locus_tag="P5241_00195"
     CDS             complement(24160..24429)
                     /locus_tag="P5241_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32021.1"
                     /translation="MDKQDIQKLQKEILESIASSNDSVGLEKLRLKYLSRKGQIALLF
                     EQFKNLDITTKKELGPLLNELKHVVIQSFNDAKNKFIDNTPKDTF"
     gene            complement(24699..25049)
                     /locus_tag="P5241_00200"
     CDS             complement(24699..25049)
                     /locus_tag="P5241_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRV32022.1"
                     /translation="MRIDLLLCFIKVNIINKNININIMIKLNKTPKQLERYFKGLANH
                     HRIEILLLLYNDDGLILEDITNSLGINFRTASEHTRRLVQAGLINKKYNGRQVRHSLS
                     PYGKLCIKFLEQFS"
     gene            complement(25140..25514)
                     /locus_tag="P5241_00205"
                     /pseudo
     CDS             complement(25140..25514)
                     /locus_tag="P5241_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
     gene            complement(25545..26384)
                     /locus_tag="P5241_00210"
     CDS             complement(25545..26384)
                     /locus_tag="P5241_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRV32023.1"
                     /translation="MKLASAIVTESGSRTCHSAIVGRELGVPTIVGVKDITKILKNGD
                     KITVSCAEGEEGKIYDGFVDFEIKKIDISKIKKPDVKIMMNLGEPNQAFSLRFLPNDG
                     VGLAREEFIIVNDIKIHPNALINYKKLTPALRAKIDKLTKGYKNKTDYYINSLCRGIA
                     KIGAAFYPKDVIVRFSDFKTNEYRSLIGGELYEPHEENPMIGWRGASRYYDPRFIEAF
                     KLECIALKKVRDDFGLINVIPMIPFCRTPQEGRKVIEIMEECGLKRQSASISINTDPN
                     CSH"
     gene            complement(26394..27713)
                     /locus_tag="P5241_00215"
                     /pseudo
     CDS             complement(26394..27713)
                     /locus_tag="P5241_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            complement(27864..28022)
                     /locus_tag="P5241_00220"
     CDS             complement(27864..28022)
                     /locus_tag="P5241_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32024.1"
                     /translation="MPSGKSVGTKEAITKNIDEALKNIKDIEKQLIGKNIFNQNEIDN
                     ILMKNLQQ"
     gene            complement(28167..29768)
                     /gene="gpmI"
                     /locus_tag="P5241_00225"
                     /pseudo
     CDS             complement(28167..29768)
                     /gene="gpmI"
                     /locus_tag="P5241_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306237.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
     gene            complement(29751..30347)
                     /gene="eno"
                     /locus_tag="P5241_00230"
                     /pseudo
     CDS             complement(29751..30347)
                     /gene="eno"
                     /locus_tag="P5241_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; internal stop;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            30586..30657
                     /locus_tag="P5241_00235"
     tRNA            30586..30657
                     /locus_tag="P5241_00235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30618..30620,aa:Met,seq:cat)
     gene            30821..30891
                     /locus_tag="P5241_00240"
     tRNA            30821..30891
                     /locus_tag="P5241_00240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30853..30855,aa:Met,seq:cat)
BASE COUNT        10246 a         5239 c         4028 g        11626 t
ORIGIN      
        1 taatatatta ttataatgta tttgttaatt tattgtaatt atatcataat ataggggggt
       61 ttgcaactat tgagagatta gtaataggca ataaaaggtt aaaataatta tacaatgtat
      121 cgttgattaa tatattatga ttatatcata ttgataaaaa taaaacaata gatgatggga
      181 gatataataa ttttaattgg gtaatgtttt gtaattatga tataaaaaga cactgaataa
      241 tgacgataat aataaaaagt tttattactt ttgattttaa acttttaact tcaaattttg
      301 gtatatatta taaacagcta tagcaccttc ggcgcaagca gtaacaattt gtttaaaccc
      361 taaatgatta ttagtaatat cccctgctgc atatacacaa tcaacagtag ttttttgatt
      421 tttatctaca ataatatatt ctttatcatc taatcctata tttaaatttc taaataaaga
      481 aatattaggg actcctccaa tctctacaaa aataccatct attgccaatt cttttgttcc
      541 attaaattca ttctttaaaa taattttatt aacaaattta tctccaataa tttcagaaat
      601 ttcattttct aatattatag taacattatt tctattacgt aaatttgata ttctttcttg
      661 cgatccttta atttcattac ttctaacaat caaataaaca tgtttacttt tttctgctaa
      721 taattcagct gcagaagtag cactatctga ccccccaaca acaccaacaa ttttattaac
      781 ataaaataca gcatcacaag tagcacaata atgtactccc ttgcccataa atttatcttc
      841 gcctttaata tttagcttcc ttttgctagt gccaagtgca attaataaat ttcgtgtttc
      901 atattgttgt tcgtttttaa gaaaaacttc aaaatatttt tctttttgta aaatacgaac
      961 tgcttctgat tctattggtt tgatacctaa tgaatttagg tgctgtttaa aattatttgt
     1021 caaatcaata ccactaatag atgtatatga aggaaaatta gtaatgtttt ctggatatat
     1081 catcatccct cccaaaacat cgcctactaa taaataattt aaattatatc tctgggcata
     1141 tatagaagag gtcataccag cagggccagt gcccaaaata attaaatcat acataataaa
     1201 agtttaataa atagtatacc aatattctga taataattgt aacaaataaa cacagaaaaa
     1261 tcaaatatga ataaatcaaa ttataataag agagccgtct gtaggaatcg aacctacgtc
     1321 tattgtttac gaaacaattg ctcgaccact aagctaagac ggcaatatat agtattttat
     1381 tttgttataa ttatattacc tgtttttaaa atagcaatag tatgttcaaa atgagcagca
     1441 tactcgccat tagctgtaga aaaagaacca tcagggtttt caataatatc atcagtacca
     1501 tatgtagcca ttggttctat tgccaaaaca tatcctgctt tgagaacaac tccagaatga
     1561 ggcataccaa aattaaaaat ttctggatcc tcatgagctt gatagcctac tccatgacca
     1621 actaagtttt taatgggcaa caatccatat tttgtcaaag tagattgaat tgtataacca
     1681 atatcaccta aagtattacc tgccttggta accaaaatag ctttatataa agattccttg
     1741 gtagctttta ttaatttttt atattttaaa catatatcgc ctactggcac agtaatagct
     1801 gtatcagtat ataatccttt atataccata ccaatatcta atgatacaat atcgccttga
     1861 tttaaaaaag ttttattgct tggaatacca tgaactataa tattgttttt agatacacag
     1921 agagatgcag gatattcttt agacataaaa tcagctttat aatgtaaaaa tgatggttta
     1981 ccacctgctt gtttaattaa atcaaaagcc attttgtcta atatatcagt gcttataccg
     2041 ggatatattg aattggcaac tgtttttaag attttggcta aaatatgccc cccttgtttt
     2101 aatatcttta tttctttttc tgttttaatt gttatcacaa aaataactta tgattatata
     2161 acttttaaca taacatggca aatattattg ttttaaatat acataattat aatacaacaa
     2221 atctaaaaaa tttaaaacaa aaatttaata ttatgataga aaatttttaa ttacctgatc
     2281 aattctttga aacacttctt ccagactacc aatcccgtct attgtatgca acattccatg
     2341 gtcttgaaaa aaattgattg ttggcataat ttcattatga aatactttaa tgcgattttc
     2401 tacaacttct ggtttatcgt cattacgaat ggttaaaata ctttgatcat ctggacactt
     2461 agtaatatct ggcgtaaaag ccaaagaata agttctacca cattttgggc aataacgacg
     2521 aatagctgaa cggtgtctaa tttcttcatc tgggatatct atataaataa caagaatttt
     2581 tatttttaat caatcaaaat attctaacat tgcttcggct tcaagcaatt ttctcgggct
     2641 accttcaaat aaaatactat catctgctga tagtgatatt aatttattca tccaagcttt
     2701 aatagcaata caaataggga cagctgctcc tgaatttaaa tcctgttcaa ccttttgggc
     2761 aataaaacgt tgatcttgtg atacccaaga tcgcaattcc tcacctacgt tgatatgaac
     2821 catcttatat ttttcaacaa tttttaatgc ttgggtgcct ttaccagaat taggtttacc
     2881 caaaagaaat ataaccataa cttatatact aattaataat tttaaaatga gaattctaaa
     2941 atgagttgtt aaaattaaaa gtaattaaat attaaacatt acttttaatt ttaaaatgag
     3001 aattctaaaa tgagttgtta aaattaaaaa attgttatta tatctctaaa ttctaatttt
     3061 ggttaatagt catactctct catagtaatt tgagcttcaa cttcacgctt aatttctata
     3121 gccacagcca ctaaaattaa aatagatgta ccacccatat taaaagttgt cactttggtt
     3181 actttttcta aaatcattgg caaaatagca ataaaggcta aaaataaagc accaaaaagc
     3241 gtaatgcgac tagaaagttt ttgaagaaac tcagcagtag tttgtccagg tctatatcca
     3301 ggaataaacc ctcccatttt ttgtaaatta ttagcaattt ctttgggttc aaaagtaata
     3361 ctggtataaa aaaatgtaaa gaaaaatact aacaaaaaat atactgcccc ataaataata
     3421 ctattattaa acatatttac taaaaaagta ccgagtttaa ctacccagcc aatcttggca
     3481 attattagca attgaccaat aaaactcgga agcattaaaa tagacatagc aaaaataata
     3541 ggaataaccc cagcctgatt cactttcata ggcaaattag tagtggcgcc tccatacatt
     3601 ttattccccc taacatgctt ggcataagac acaggcactc gcctttctcc ttctgtaata
     3661 atgacaatag caacaatcat agccaaagcc ataacaaaga aaataattaa ccccaatatt
     3721 tgagataaag tagctgtttt gtaggcaata aaagtattaa taatactcaa aggaatttct
     3781 gagacaatac cagcaaagat tatcatagaa ataccattac ctaatttttg ttcagtaatc
     3841 aactcaccta tccaaactaa aatcattgaa gctgctgtca aaactataat attatttata
     3901 agagacagag tactatcaat agtaattatc ttttgaaatt caaataaacg taacatagca
     3961 aaagcttgta atactgctaa aggaatagat aatcaacgag ttcaattttc aaattttcgt
     4021 ctgccttcag ccccttcttc ataaaatgac tcttttaatc tcggaataac catagtgagt
     4081 aattgtaaaa taatagaact cgtaatataa ggccctacac ccaaaagggc tatggacatt
     4141 ttggaaaaag ctccaccggt aaaaatatta aataatccaa aaatttgatt ggattgtaaa
     4201 aaagttttta aattggctgg acttacgcct ggcataggaa taattgtaag caaacgaaat
     4261 accaaaagta atcctaaaac atttagcatt ctttttctta aatccggatt tttaaaaatt
     4321 gtaataagat ttttaatcat aattatttat tttgctaatt attttttagt agatttaatt
     4381 atgccaccag ctttttcgag ttggtttttg gcaccctgtg acatattgat atctataatt
     4441 gtatattttt tgcttaaaac atttttagag ccaagaatct taacattgtc ttttactgat
     4501 ttaattaaac ctttttcttt tagagatttt ggagatataa tagtattatc attaaaattt
     4561 ttggcaatag tatctaagcc aatatttttg gttcttttgg gaatagtttt attaccacta
     4621 ccacgacgtt taggaatacg caaaaacatc acgcgcaatt ctgaatgaat tttgcgtcca
     4681 gcacgagact tttgtccttt ttgtcctcga ccagaataag ttcctctttt accaccacgt
     4741 ccaacacgtt tggctcgttt tcttttagat tgtaacttcc gattgtttat ctgcatattg
     4801 ttctatatta tcattttctt taaaattatt tttattttgt gtgtcgcctt tctcattatt
     4861 tattttattt tctttatttt gattagtatt caatttagaa atagccaata aaaaagccct
     4921 ggtattattt acaacattat gtgaacctcc taaaattttg gtagtgatat ttttaatatt
     4981 agtcattttg ataatatctc taagtacccc gccagcaatt atacccttac ctgctttggc
     5041 tggttttaat aatactttac tggcacaata tttacctgaa accgcgaaag gaatagtatc
     5101 atttttgata ggaatagtaa taatgttttt cattgcttct tttttggctt tttcaatagc
     5161 ttgaggcaca tcactacctt tggctatacc aaaaccaatt ttgtctctat gattacccaa
     5221 taatattgct gcacgaaaag ataatttctt acctccacct gtaacacggg ttactcgttt
     5281 aatttctaaa ataatttgat caaaatcttc attacttgat ctgggtttac gagaaacaaa
     5341 tttagatgta gatctaaaat tagaagaatt ttctttgggt ggcaatggat tacttgtttt
     5401 ggtgttagtt tttaaattgt ttggattatt ttttaacata attaaaatat tataccattt
     5461 tttcttaaac tatcggccaa agcttgaact ctaccgtgat attgatatcc accacgatca
     5521 aaaactgctt tggtaatatt tttagctttc attttttccg ataacattgt accaagcatt
     5581 gatgctatat cagttttctt tttgatatct ttgatatcat gatcagaaat gcttagcaat
     5641 gtaatttgtt tttcgtcatc aataacttgt acatacatat gtttattaga acgaaaaact
     5701 gaaatacggg gtttatcaga agtacccgaa attttagatc gtattctttt atgaatgtgt
     5761 ttacgttgta tttttctttt tttggctata tccataataa taatttaaac attaaaataa
     5821 ttttaagaac cagacgaggc aactttttta ccagttttac gaataatatg ctctccttga
     5881 tagtgaatac ctttgccact ataagggttg gttttcctta atttacgaac attagcagca
     5941 aaatcgccta cattctgttt ggaaatacct ttgatagaaa ttacattttt ttctacactg
     6001 acttctatac ccttaggaat atctacaaat actggatgag aatatcctat atttaaaact
     6061 aatttgtcac catttacgtc ggctttatat ccaatacctt ctattactaa ttttttttca
     6121 aacccttctg ttactccaat tatattattt tgaatcaaag cagtagcagt ccctcacaaa
     6181 gccgacttta aatgatcaat ttttgacgga ggtataattg aaagtttatt tttgttctgc
     6241 actatattaa aaccttctgg taatatagat gtaagcatac ccttgggtcc tttaatagta
     6301 acctctttgt tgtttacaat aagttcaaca tcatctggaa tagttaaaat ttttttacca
     6361 atttttgaca taaataaaat ttaataaatt aaatttttat aaaataattt gctaataaat
     6421 ctcaaaaagc acttcgccac ccaaattcag ttgtttagct tctctatcag acataatacc
     6481 atggggagta gatagcaacc ttaaaccaaa tcccttttta agagggtgga tatctttggc
     6541 cttaatataa acacgacaag agggtttcga aatgcgtttg acatctttaa tagcaggaat
     6601 attgttatta tatttgatta ttacttcaaa atttttatga actccttttc ctatttcagt
     6661 aaagttttca ataacatttt ctttttgtaa tgttaaaagt atctcttttt ttaatttaga
     6721 gactggaata ttaaccattg gtttattaat ggcgctagca tttttaatac gaattatcat
     6781 atcggcaatt gggtcagaca tacaaataaa tttaaaagtt taaaagtata agagtataaa
     6841 tatttatcaa cttgattttt taactccggg aatttctcca cgagaagcca tttcacgaaa
     6901 acaaatacgg cacaaaccga aatcgcgcat ataacctctc tttcgaccac aacgaaaaca
     6961 acgcaatact ttgcgagttg aatatttagg agttttttta gatctggcat aagtagattt
     7021 ttttgacata ataaataaat ttaatatttc gagttatttt gctaatggaa atcccaatac
     7081 ttcaaatagt ttaatgcctt cttctttact tttggcatta gtagtaaaac aaatttctaa
     7141 accaaaggga attttaatag tttctttggc taattctgga aaaatttgag cctctttaat
     7201 accatagcat aaattacctt tttgatcaaa atgtttttta gaaatacctc agaaatcacg
     7261 agttcttggc aaagtaatat taacaaaacg atctataaaa tcattcattc ttttacctcg
     7321 taaagtaacc atcaagccca caggcatacc ttcacgtaat ttaaaaccag aaactgaaac
     7381 acgggcattg catacttttg gtttttgacc ggttatagcc atcaaatatt cacttatttc
     7441 tttaacaata ttgtcttgtt tttcagctgt atgagtaact aatttaccaa tcccacaatt
     7501 aacaactact ttggtaatat gtaaattgtg taattgtgct ttatttttat ttaattgttc
     7561 tttgtaacta attttcgaca taatatatat taatttagtt ttgctttaca tttgtgacaa
     7621 attcttattt taactttttt attattttta tctgtttcaa taataaaacc aattcgagtt
     7681 ggcttaccac atttggggca aattaacata gctttagagc tacaaaatgg tcttgataga
     7741 actacaatct gtcctttttc tccttctttt cgaggttttc tatttttttt ataggtatta
     7801 aaaccttcta ttaaaatcgt attatttttc ggaaaagtac gactaatcgt gccagttttg
     7861 cctgcgtctt tgccttttat aatttttact ttatcacctt tttttaattt cattttaatt
     7921 tatttatttg ttaccaaata caaattctaa aatatattaa tcggtaaatc gtctatccag
     7981 tttattatac taattctgga cccaactcaa ttattttatt aaatccttta tctttaattt
     8041 ctctgggtat gggaccaaaa atacgggtgc caatcggttc ttttttatca ttaatcaaaa
     8101 caactgcatt gtcatcaaat cttatataag atccattagc tcttcgataa ttcttgcgtt
     8161 ggcggacaat aacacagcgt actatatctt tctttttcac agattttctt ggtttggcaa
     8221 cttgaacagc agccataata acatcaccta tttgaccata acgatgataa gaacctccac
     8281 gtatttgaaa acaacgtaca atctgtgcac cactattatc tgctactttt aataatgaac
     8341 gaagttgaat cataatctat tttatagttt atagctacta actgttagct taaatttatt
     8401 aaatatatat ttatactaca atatttttgt ttaaaaagca agcctttggg tttaacacaa
     8461 cacgaaaaca ttattacaat tgatttattg gtattttaag ataacaatag tacttaacat
     8521 ttagctatca gctaaaaaat tcaaaaaact atgtttatct gtattgctgt ctaactatgt
     8581 atatttatgt tttcttttta ttcgtgtcaa cccgcgtctt gttgtaattt taattatatt
     8641 ttaagagact tttttaacaa actttcaaca ttttgttttg gatataggtc ttgtttcttc
     8701 tataatgact ttatcacctg ttttgtatat tgaattaggg ttatcaactt ttactttttt
     8761 agacaaaaca aaaaattttt tatatcgagg atgtttttgg aaaatatcaa ctttgactac
     8821 acaagtttgc gtcattttgt cagaaactac tattcctttt aattgtctag gcatgttttt
     8881 gacttaataa ttgattaata atagtagaaa tttgtgcgat atctttttta agattaaata
     8941 aaatttttac atttttaact tttcccattt tcaagtcaaa ttttgcttgt atagcttgct
     9001 tttgtttctc cgttaaaagt ttttgtaatt cgtttaagtc ttttttttct aaatctttta
     9061 ttttcataat atagcaaatt taattcatta ttaattcata ctagcagaac tattttcttt
     9121 aataattatt ttagttttaa atggcaactt ataactagct tgttttaatg cttcaactgc
     9181 tttgtttgtt ggaatcccac ttatttcata aataatacga cctggtttta tttgaaatac
     9241 ataatgatca acatcaccct tacccatacc cattctaact tcaggtggtt ttttgctaac
     9301 tggtttatct ggaaatactc taattcaaat ttttcctcct ttttgaatat aacgtaaaag
     9361 aacacgccta gcagcctcta tttgacgact cgtaatacgt gatacctctg tagcttttaa
     9421 tcctactgtg ccatattcta aaacagtacc tcgattttca aatgaacgag taagagatct
     9481 acctttttgc atttttctat attttgtacg tttgggaaca aacataatag tttaatataa
     9541 tttatagttg ttgactattg accttttgtt tttgattaaa aacatctcct ttgtaaattc
     9601 agacttttat accaatggct ccataagtac aatgagcagt tgccatagca taatcaatat
     9661 tactacgtaa agtatttaat ggcaatttac catctttgta tcaatcgtct ctagcaatat
     9721 cagcaccatc taaacgacca gatactcaaa ttttggctcc tttggcttct ttattttgca
     9781 taattttttc aacatatttt tttattgttt tacgataagg ttgtcttttc tcgaattctg
     9841 tggcaacatc aaaagctaca atctgagcag acaaaaatgg tttgcgaacc tcttctatat
     9901 tcaaagaaat gttaatattt ttgtcaattt tattcttacg ataatatttt tctaaagttt
     9961 tttcaatggc tttctttaat tctcctaaac cttccccctg atgttttata atcataccag
    10021 gtcgagaagt ataaatagtg atatttattt tatcactctt acgctcaatt ataatttcac
    10081 tataatgagc tttggataat aatttctcta aacattttcg tataatatta tcttcttcta
    10141 atttttgtgg aaaattttgt aaatcaaatc atcttgaact tcagttttta ttaataccca
    10201 atcttttaga tattggatta atattttgcg ccatattata tataattatt ttattacttt
    10261 tctttgaatt attggtttaa cttgaccttt tttagctttt gtttgtttag tttctctata
    10321 aaattttgta tttttattgc gaggtttatt cttttcataa actgttttac tttcctgtgt
    10381 ttttgcaatc tcaggactct gcgctatctt atttaatgat tttaaagaat gtgcagtgtc
    10441 taaggtaatt acaatatgag acattctatg acgaataaca ttggtggcac catgggcacg
    10501 agggagagct cgttttaaca ttggcccttg attaacaaca atgcttttta taaataatgt
    10561 agaagggtct aaatgaaaat tattaacagc attggctttt gctgacatta aaagtttcaa
    10621 aataggagca gcacttctat tagaaatgtg acgtagctga tcttcggcac ttgatattgg
    10681 aagcccttta ataatattag ctactaaacg aactttacgt ggagcgattt taagatattt
    10741 taatgaagct gtaatttcca taatagttta tatcaaaatt tttataaatt tattttttat
    10801 tagcagaaac agatggtgca gatacttgtc ctgatgcttc tgaagctttt tgtaatttgc
    10861 ccccgtgttt aataaatttt cgagttggag caaattcccc caatctatgt ccgaccattt
    10921 cttcagtaat ttcaacatta ataaaacgtt taccattatg tacagcaaaa acaaacccta
    10981 tcatttctgg agcaatcatt gaatctcgat atcatgtttg aataggagtt ttgtctcctg
    11041 gtttcaaatt tgatattttt tttaataatt taggagccac ataaggacct ttcttcaaag
    11101 aacgagacat agttattata gtttattgct ttcagttata agctgtaagc tttttaataa
    11161 ttaaattgtt acaaaattat tttttctttt taggagaacg acgtgacaca ataaaagcat
    11221 ttgaatattt ttgttttgct cgtgttttac gacctccggt aatatttcct catttatctt
    11281 ttggacgttt tgtacctctt ggtgttttac ctctaccacc tccataagga tgatctggag
    11341 ggttcatagc cgaacctcta actgttggac gaattcctaa atgtcgtgat ctacctgcct
    11401 ttcctaaact tgctaaacta tgttcaacat ttgaaagtac tccaattgat gcaaaacaat
    11461 tgcttaaaac ttttctcatt tctgtagatg gcatttttaa ataagcataa ttgccttcca
    11521 atcctgacat aattgcatat aaacctgctg cgcgtgcaaa ttttcctcct tgttttggtt
    11581 taatctctat attgtgaatt tctgtaccac ctggaatatt ttttagcatc attctgttac
    11641 caacactaat acttgtttta tcatcacaaa gaattttgtc accaattttt aaatcttttg
    11701 gagccaaaat atattttcta actccattat tataaagaac caaagcaata aaacaacttc
    11761 tattgggatc atattcaatt ctttcgactt tgcctggaat accttgcatt gattctccaa
    11821 attctatact tctgtataat tttttgtttc cactaccttt gtgtcgcatt gtaatacgac
    11881 cagcattgtt tctgccaact gatctttttc taccaaaagt caaagatttt aatggctttg
    11941 attttgccaa attggtacga tcaatatcaa tatagtgtcg tgtacttgga gttattggtt
    12001 ttcttttttt cataaaatat attttattag ttatataatt ttatataatg tatagatcta
    12061 aatattaaat tctattttct gtccgggttt tagagtaact attgcttttt tgattggtgc
    12121 tttatgagat atatgattac gaaaatttac tggagaaaaa aactttttag atatattaac
    12181 atcggcgacc ttaacattat aaaatttttc aataaatttt ttaatttcaa ttttattggc
    12241 tttcttacta accaaaaaat ggtatttatt accctctttg tctaatgtgg ctttctcagt
    12301 aatatatgga tcacaaaaaa atgccacaaa attccaatcc tttttgacag ctgtgttcgg
    12361 tttggaaata tttgttttgt ttgttttagt agatattttt ttattagttt tgaaacgact
    12421 taaaatactt gacatattaa ttattaaaat tatttatttt tagaaaattt ttgttctaat
    12481 ataggaatag ctgtttgtaa aataacaaca ttcttataat taagtaaatc tattaaatca
    12541 atagacgaag cagtggtagc aaacgcaaat ggaagatttc tcgtagcctt gataagactt
    12601 ttattagcat catctaaaat taaatagatt gatttgttag tttttttgga tttagttggt
    12661 ataaaaagtg aaactatttt tgccatttct tttgttttgt tagcaggcaa ttttatttca
    12721 tcaataataa acaattcgct gtctttgagc ttttgagaca acaacatagc caaagtttta
    12781 ttttttaatt tctgattaac tttacgatta taattttctt cgttctgtct gggtccaaaa
    12841 gtaatacctc catgtcttca aataggtgaa cggatggaac cagctcgagc ccttcctgta
    12901 cctttttgca cccaaggttt tctacctcca ccccttactt ctcctctatc tttagtatga
    12961 gcaatgggtt gacgtcggga gcttaatata gatctaacaa cttgagaaac agaatcagca
    13021 ttaaaagata cattgaatat tttatcatta agttctattg tcccaatatt ttcttttttt
    13081 tgattatata aatttgtttt catttgtaaa atatttaata caaattattt agattctatc
    13141 ttaaccaaac ttcctttgac tcctggtaca gcacctttaa gaaaaatcaa attattatct
    13201 acatcaatat ctaaaattgt taaatttttt atagtaacag tatctccacc catacgacct
    13261 gccattttat gtcccttgat aactctctga ggtccttggg caccaattga tcctggctcg
    13321 cgtaatgcat gtttagtacc atgagtagct gaagcaccgc gaaaacctca tcttttaact
    13381 actccttgaa accccttgcc ctttgaaata ccttgaactt taacaatatc acctggagca
    13441 aattgtttta tatctataat atctcctatt ttatattcct ctggattgtc tactctaaac
    13501 tcttttttac tttttttctt tttattttta tcaacactaa tttggaccgc attataacca
    13561 tcagttttgt ttgttttgat ttgagataca gaattaggaa ttacttgtaa aacagttact
    13621 gccacaaatc cttcagaagg gtcaggaagt ctagtcattg ttaatttttt agctaaaaga
    13681 aatttcattt taaaatcttt ataaaatctt taattatgtt ggattaaaaa tgtttttaac
    13741 ctcaacaaaa aactgagtaa tactcagttt tttgttgagg cttaaaaaga atatcctttt
    13801 aaagcctcat aaaaaattaa gtttttattt ctatttctac gccagctggc aaatttaaat
    13861 ttgtcaatga ttcaataatt ttttgatttg gatttaaaat atcaataata cgcttatgta
    13921 ctcttatctc aaattgctct tgggaagtct ttttaacaaa agaagcacga ttaacagaat
    13981 aacgttttat ttctgtgggc aataaaatcg gacctattac ttgagcccca ttacgaacaa
    14041 ctatatcaat aatctgtttg cagcaattat cgataatcct atgatcatac gatttaagtc
    14101 gtacacgaat tttaatttta tctttatctt tattttctaa ggtgctagat gacattttat
    14161 taaactttta ctggttttat taagcaataa ttttggtaat tacgccggcg ccaactgttt
    14221 tacctccttc acgaatagca aatttttgtt tttcctctaa agcaattggc ccaatcaatt
    14281 taactttgaa agtagctgta tcgcctggca taaccatttc tactccttct tttaaagtga
    14341 cttcacctgt aatatcagct gtacgaataa aaaattgagg tttataaccc gtaaagaatg
    14401 gagtatgcct accaccttca tctttagata atacataaac ttctgattca aattcagtat
    14461 gaggtgtaat acttcctggt ttggcagtta cttgtcctct tttaacatct tccttttttg
    14521 tacctcttaa caataatcca acattatctc cagccaaacc ttcatctaat gttttattaa
    14581 acatttcaac acccgtaaca actgttttta tagttggagt ataaccaaca atttctactt
    14641 catcatttat atgaattgta cctcgttcta ttctacctgt aactacagta ccacgacctt
    14701 caatagtgaa aatatcttca attggcatta aaaatggctt gtctttatca cgaacaggtt
    14761 caggtatata agaatcaagt gtatctaata gttgaataat aggttttact gcgtcatcat
    14821 catagcttgt tgcctccaaa gcctttaagg ctgaacctcg aataattggg gtatcattac
    14881 ctggaaaacc atatttattt aataactcac gaacttctgt ttctaccaaa tcaatcattt
    14941 ctggatcaga aacagcatca catttattta aaaatacaac aatagctggt acaccaactt
    15001 gtctcgccaa taaaacatgc tcacgagttt gaggcatagg accatcagca gcagatacaa
    15061 ctaaaatagc tccatccatt tgagcagaac cagtaatcat atttttaata taatcaacgt
    15121 gtccgggaca atcaatgtga gcatagtgtc tcttgtcagt ctcatattct gagtgatgaa
    15181 catttatggt aatacctcga cttttctctt ctggagcttt atcaatatca ttaacagata
    15241 ctgctttggc aactgtacct tttaaatgaa gagtattcaa aattgccgct gttaatgttg
    15301 ttttaccatg atcaatatga ccaatagtac caacatttaa atgagatttt gtccttacaa
    15361 atttttcttt ttctgccatg ttatatattt aaatttttat taattttatt attaaagcct
    15421 ttttctaagg gtatttttaa tattaccgca tttttgttta aaagtcaaga cctttaaaaa
    15481 ttccaaaaat gaattatata ctaattatac ttcttttttc ccgataatac tttctgcaac
    15541 aagtgcaggc acttcttgat aatgatgaaa ttccatatta tatgtaccac gcccctcagt
    15601 tattgaacgt aaattagtaa cataaccaaa catttcggct aaaggaacaa aagcatcaac
    15661 tactcgtaaa ttataacgat caaacattcg ttcaattcgc ccacggcgtg aattaatatc
    15721 accaattata tcacctaaaa atttttctgg agcagttact tctactttca taatgggttc
    15781 caatagcact ggattggctt ttttggctcc ttcctgaaaa gccattgaac cagcaatttt
    15841 gaaagcaaaa tcagaagaat caacttcgtg aaatgaacca tcatataaag taactcttat
    15901 atcagaaact ggatagcctg ctaaaatacc tttatccaaa gcttcacgca cacctttttc
    15961 tacagaagga ataaattctt gaggaataat accacccttt atttgattaa caaactcaaa
    16021 acccttgcct cgttctaatg gttcaagctt taatcaaaca tgaccatatt gtcctctacc
    16081 accagattgg cgaatatatt taccttcaga ttcaacaaca tcgcgtatgg tttctttgta
    16141 agcaacttgg ggtttaccag tatttacatc tatattaaac tcgcgacgca atctatcaac
    16201 aataatatct aaatggagtt cccccatacc tgaaataatt gtatcagaag tattctcgtc
    16261 tgttttgact ttaaacgttg gatcctcttc ggataatttt cgtaaagcta aacctaattt
    16321 ttcttgatca tttttagttt taggttcaag gcgcaaagaa ataacaggtt ctgggaaaga
    16381 aattgattct aaaataattg ggtgagcttc atcacaaaga gtatcgcctg taccagtaga
    16441 ctttaaacca acagtcgcca caatatctcc ggcttcaaca ctatcaattt cttctcgttt
    16501 atttgagaac atacgaatca aacgactgat acgttcttgt tgattttttg tactattata
    16561 aagatacgaa cccttagtta gcgatcctga ataaacacga aaataattta aagtaccagc
    16621 aaagggatcg gttgcaacct tgaatactaa tgcgctaaat ggttcatcgg gattggcatg
    16681 acgaataatt tcttcaccag tttttggatc tacgcctttg actggaggca aatcaagagg
    16741 agaaggcaaa tattctacaa cagaatctaa catacactga acacctttat tttttaaaga
    16801 agaaccacac ataactggaa ttaatctata ttcaattgtt gctttgcgca acacttgtct
    16861 taattcttct gcatcaatag gcttgttatc taaatatttt tgcattaaaa cttcatcttc
    16921 tgaagcaatt ttttctaaca tttcttgatg atatttttga gcatattctt ttaaatcatc
    16981 tggaatatca gatagcacaa cattttctcc atgttcgcca tcaaaagaaa tctttttcat
    17041 tgtaatcaaa tcaataacac cggcaaaatt atcctcttca cctataggaa tattaatagc
    17101 aagggcattg ggagttaatt tttcttttat tgattgcata tcattttcaa aatttgctcc
    17161 taatctatcc attttgttta taaaacaaat ccggggtact gaatatttat cagcttgtct
    17221 tcaaacagtt tctgattgtg gttcaacacc agctacacca tcaaaaacta caactgcgcc
    17281 atccaaaaca cgcagagatc gttgaacttc ggctgtaaaa tcaatatgcc ctggagtatc
    17341 aataatatta attttgtgtt taaaatcaag gttattattt ctataagttg gtgttcaaaa
    17401 acaagtagta gcagctgaag taatagtaat tccccgttct ttttcttgaa ccattcaatc
    17461 cataatagcc tctccttcgt gaacctcacc aattttatga gaaagccctg tataaaataa
    17521 aactctctca ctagtagtag ttttaccagc atcaatatgg gcaataatac caatatttct
    17581 tattttgtct aaagaataat ccataatatt aaattaaaaa aattaaatat aaaataccaa
    17641 gttagcgtat agcttataac ttaaaaaaga aataaatacg catgtttgtt tattgataac
    17701 gaccaaaatg cgcaaaagct ttattagcct cagccatttt ttgagtatca attctctttt
    17761 ttacagccgc cccttcatta ttatatgctg aaattatttc ttctgctaat ttgtaagcta
    17821 ttggttttcc ttttttggct ttagcagcat ttaatattca acgcattgcc aatgttgttt
    17881 ttcgttctcc agatacaggt ataggaactt gataatttgc tcctccaata cgaacgcttc
    17941 gaacttctaa attaggcgca acatttgtaa gcgcatattc taaatatttt actggatctt
    18001 gttttgtttc tttttctaaa atctccaaca ttttataaat attcttcctg gcagtagctt
    18061 tttgtcctcg tctcattgtt atatttataa attttgatac agtaatactt tgatagtgaa
    18121 aatctggttt atattttttt tgatcaataa tcaaatccat aattatatca atttatagtt
    18181 ttttagaata ttttatttag atctcttagc accatatttg ctcctttgct gttttctatt
    18241 atctacacct gcagtatcat atttacctcg aataatatgg tatcttactc ctggtaaatc
    18301 ctttactcta ccccctctaa tcaaaacaat agagtgctct tgtaaattat gaccaatacc
    18361 aggaatatag gcagtgacct ctaagccatt tgatagtttg acacgagcaa ctttacgtaa
    18421 agcagaattg ggttttttgg gagtcatagt aaacacctta gtacaaactc cttttttaaa
    18481 aggagagcta taattcttaa tacgattttt cttaatatta aaagcgaacc ccaaacctct
    18541 tgttttggat tttttaataa ttggtgatct tccttttttt cttaattgat ttactgttgg
    18601 cataattaaa tttaattgta taaaataaaa atataaatct caaaaaacca aagttaaaag
    18661 tcaaaatctt aaagtgataa tacaaaataa aactttttag atttaagata aatctgtgac
    18721 cttaaacgta taattttttg atattgttat attatcataa aactttataa aaatcaatac
    18781 ctgctagcac atacactgat ttgccacaca aaataaaaaa acaattttta ttttcttcgt
    18841 ttgctaactt tttactttca atgtctatta ttaaaaaatt agaaattaaa aattgttttt
    18901 ggtatacttt gtattttatt agatgtctaa atttttaact tttttagcat aagcttcgat
    18961 gaatttttta cgtggtaata cttcatcacc cattaaaata ctcataagct tatcagcatc
    19021 ttgggcatct tttacagtaa cctttaataa aactctattg gcaggatcca ttgttgtttc
    19081 tcacaattgt tcagggttca tctctcccaa acctttgtat cgttgtatac taataccttt
    19141 atagttgtcc atattcaatt cttgttctga atttttatgt tcttcttgat gatcttgttg
    19201 ttcttcttca ctttcaacta caagcttaat tttacctttt gttgttttat tatcttttat
    19261 ttctttttta tcacttaggc ctataatttt ttgtaaaatt tgttcttttt gactatcatt
    19321 ataagcataa taaatatctt tgcctttttg aatacgatat aatggcggtt gagcaatata
    19381 aatataacca ttttcaacta atggcttaaa atatctataa aacaaagtaa gaagtaatat
    19441 tctaatatga gcaccatcgc aatcagcatc agcagctaaa ataattttat gatatcgcaa
    19501 tttattcaaa ttaaaatttt ctgaaatagc agtacccaaa gcaagaatta tcgcttttat
    19561 ttcttcagat gccatcattt tgtctaatct tgccttttct acatttaaaa ttttacctct
    19621 taatggtaaa actgcttgaa tatttctatc tcttgcttgc ttggtactac cacctgcgct
    19681 ttcaccctca acaataaata attctgattc tgaaggttta ttagaaatac aatcagcaag
    19741 tttacctggc aaagtagctg tttcaaaaac tgatttacgt aaaacattat ctcgtgctgt
    19801 tttggcagca tttctagccc gagcagaaat catacatttt tctaaaatac gttttgcatc
    19861 atcaggattt ttttctaaaa aatcacgaaa agcttttgac ataacttgat caacagcaac
    19921 acgggcatct ggattaccca atttagcctt ggtttgtcct tcaaattggg gttcgtgtaa
    19981 tttaatagaa atcaaagcca ctaacccttc tctaacatca tcaccagtca gtgatatatt
    20041 ttctttattt ttatcactat gctcttttag ataatcattt aacacccgag ttagggcagt
    20101 acgaaatcca gtaagatgca tacctccctc tggggtttta atattattgg caaaactaat
    20161 ctctacactt tctatatctt cggtataagc caaagctatc tctatttgca tatcctggca
    20221 ctcatcctta acataaaata attgtttttg caatgatttt acctcgcgat caaataataa
    20281 aaattgtaaa aatgatacca atcctgaact aaaataaaat acatactttt tttcattatc
    20341 tttttcacgc aaatcttgta gagtaatttt aacttgttta gccaaataag cttgctctcg
    20401 caaatgatct aatattgttt tccaatcaaa ttttatttcg ggaaaaattt cctcgtctgg
    20461 ctcaaaagta attttagtcc catgctttcc tttttctcct ttttctttat taactttaga
    20521 aataggttta ccttgtttat attcttgtca atatacaaaa ttatcacgat atacttccgc
    20581 ttttaaatat tttgataatg catttactac agatacacct acgccatgca acccaccggc
    20641 aattttatat gacttaccac caaattttgc accagcgtgt aaagtagtca aaactgtttc
    20701 gagagctgat ttttttgtct gtttatgaat atccacagga atacctctgc catcatctaa
    20761 aacagaaact ctattattag gtaataaact aatttctatg tttttacaaa aaccagctgc
    20821 tacttcatct aaactattat cagtaacttc aaaaattaaa tgatgtaacc cagataaacc
    20881 cgtagatcca atatacatac ctggtctacg tcttacaggc tccaaacctt ctaaaacata
    20941 aatatctttg gctgtataac cagacacatc attgtggata tcatctatgg taggtttagg
    21001 aatatttaca ggcttttggt ctttattttg tttagacata atttgtattt attgtgatta
    21061 attttcatgt tatagattat aaatctagac atatattatc ttatagatca gcctttttgt
    21121 ttcaaatttt ttacaatttt atcaaactcg tttttaattt cttgatcagt aagagtatga
    21181 tcatcacttc taaacataag atggaaagaa atacttttgc ctttgtctaa tccttcatat
    21241 atatcaaaaa tatcaatgtc agctaaatta ttggcattgg attgctgaat agctaaatag
    21301 atatcttgaa tcaaagtatt ttgtggcact aaaatcgaca aatctttaat actagaagga
    21361 tattttggta atgggttaaa ttcaatttct tgttttacta atttaaataa ttcttgtaaa
    21421 tttatttctc ctaatataac aataggtttt ttatactcta tggtaatatc atatatatct
    21481 aaaatcttgt ttgaaggtat tattaaagaa gctaaatatt tattatcatt ggtataaaaa
    21541 ataatattgc tgttattgtc agcaaaaatg ccatataatt tgtctaacaa attaggtaat
    21601 ttattagaag taaaatagct atgttcgcta ataccaaact tgctaaacaa agattccaaa
    21661 atacccttgg ctttaagcaa tcaatcagat acgcgaacag aattttgaga attataaata
    21721 gcaaaactat agcaatcaga ttctacaaca gtattgtttt tgttgagaaa attttttgaa
    21781 atttcaaaaa attgcgctgt tggaaaagtt ttaatatttg tgctaacagc acgcaaacta
    21841 gatactaatg aaaaaggacg aagatataaa tattcggagg attgaggatt ttttatttta
    21901 acaagattgg gcaatattga tttatccaaa atatttattt cttttgcacc aataaaatta
    21961 tattcatgaa tttcagacaa tcccaaaccc aaggaataag ttttaaggta ttgcttaaaa
    22021 ttgtaaaatt cgttatcctt aggatatgat aaagtaattt gtggggcgat gaatggtata
    22081 ttattaaacc cgaaaattct tcctatttct ccaatcaaat cgtcttcatt ttgtaaatcc
    22141 aaacgccaat atggtataga aactaaataa agtttaggat ttcttttaac aaattgaaat
    22201 tctaatttat gtaaaatatt ttccaaaaat ttaacatcta cattaaaccc caaaaaacga
    22261 taaatttttt ctaaattcaa caaaattatt gttggtttgt tgttttttga atttgttcta
    22321 atagcgggtt ctataattgt accattggca tattttttta ttaaaaatgc cgcttttaaa
    22381 agagagacat aagttaattc ttgtggtaaa ttatgagcaa aacgacaaga agcatcagta
    22441 gataaattaa gatatttgga gcttttataa ataagtgaag aattaaaatt agcactttca
    22501 agtactatat ttttagtgtt tttggtaata ccggcatatt cacctccttt gataccagca
    22561 atggccaatg gcttatattg attggcaact actaaaatgt tttcatttaa ctctattggg
    22621 gttccgtcca aagtcgtaat tttttctcca gccctagcgc gccttatttt tattaatgct
    22681 gaattgctat gatgcttgtt tatactgtta agctcgcgtt tatctgctgt ttggtctatg
    22741 tttattcgat cataatcaaa cgcatgcatt ggttgattat attctaacat tactaaattc
    22801 gtaatatcaa caatattatt aatggaacgc aaaccacaag aaagtaattt tgattgaatt
    22861 tctttgggcg aaggcttgac ttcaacgttt tgtattaata gcccacaata ttgaggacaa
    22921 tcatttggat cgtcaacttg tatatttaaa taagaatttt tggcatttac aaatttaatt
    22981 tgtggtattt tgattaattt ttgatttaaa tttaaatcta aacaagtagc aatctctctc
    23041 gccaaaccca aatgtccacc caaatcagca aaacgattaa caggtatatc aatgtttaac
    23101 acaaaatcag acccatgttt tttatatgta gtttctccta taaaataatt caaaatctct
    23161 gctaattttt tagcttctgg taatttattg ggaatatatt cttttaacca attatatgaa
    23221 tactgcataa taaaatgaaa taagtattaa aatctataat ataataaaat taacataaag
    23281 gacaataaat aaataattaa ttaagttaaa gtaaaattag aaagagttgg taaaattaaa
    23341 aagaggtcag tcggaggatt gcttttaaaa ttgtttagta aatcgtaaat cacctgaatt
    23401 aaacaatctt acatcattaa taccatattt aaccatagcg aatctttcta atcccaaacc
    23461 aaacgcaaac ccttgataat cataattcgc tcaattagca taacgatata atgctttgtg
    23521 caccatacct gcacccataa cttctaatca accagtacct gcgcataaat tacattgttt
    23581 atttttgcca tggcatttaa agcaagaaat atccatttgt aatcccgggc taacaaaagg
    23641 aaaataactg ggcaaaaatc taatttttat atctttgcca aaaatattct ttacagcctc
    23701 tgacataata aatttgaaat tagataaact aatatttttg tcaatcatta atccttcaat
    23761 ttgtttaaac tggaattcat gtctagaatc tgtggcttca taacgataac aagtaccagg
    23821 tactattatt ttaattgggg gagtatgttg ctccatatac ctcatttgta cggggcttgt
    23881 atgagtacgt aataataaat ttatagcttt tcttggataa tttttagaat aattgcttaa
    23941 ttttaaatct ggctctaacc aaaaagtatc ccacatatct ctagccgggt gatctttggg
    24001 aatattcaaa gcatcaaagt tgtaatattc actttctact tctggaccct caacaacttc
    24061 aaaccccatt ttggaaaaaa catttacaat ttcacgctcc attaaagtat tgggtaatag
    24121 ggaacctata tctaattttg tgcctggcat agtataatct caaaaagtat ctttgggcgt
    24181 attgtctata aatttattct tggcatcatt aaaactttgt ataacaacat gtttaagctc
    24241 atttaataat gggccaagct cctttttggt tgtaatatct aaatttttaa attgttcaaa
    24301 taaaagagca atctgccctt ttctagataa atattttaaa cgtaatttct ccaaaccaac
    24361 agaatcattt gaagaagcta tagattctaa tatttctttt tgtaattttt gaatgtcttg
    24421 tttatccata tgattgatta ttatatatta attttatcaa atttttgata tttatgcaag
    24481 cctttatatc tacattttat acttaaatct aagttttata cctcaatcta agtttcacaa
    24541 acacatatac ctaaatctaa gttttatacc tcaatctaag ttttgttttt acataattta
    24601 tacctaaatc taatataccc acacctaaat tttactatta cggacattta catattcttg
    24661 ggaatatgta aatgtttgta acgaatttat aaaaactttt atgaaaattg ttctaaaaat
    24721 ttaatgcaca gtttaccata gggagataaa ctatggcgta cttgacgacc attatatttt
    24781 ttattaatca atcctgcctg aaccaatcta cgagtatgtt cagaagctgt tctaaaattt
    24841 atccctaaac tattcgttat atcttctaaa ataagtccgt cgtcgttata aagtaaaaga
    24901 agaatttcta ttcggtggtg gttagccaaa cctttaaaat atctctcgag ctgtttgggg
    24961 gttttgttta attttatcat aatgtttata tttatatttt tgttaataat attgactttt
    25021 ataaaacata acaacaaatc tattctcata aaataacaaa tctatgctta caaacattta
    25081 catattccca agaatatgta aatgtttgta actgtggttt tttgttagaa tttttttgtt
    25141 tattccaatt ctttgatagt ttttagaata gaatcaggat ttaaagatat gctctcgata
    25201 ccttcgttaa tcaaaaattt agcaaattca ggataatcac taggcgcttg tccacaaata
    25261 ccaatatact tatttctaga cttacatttt tgaataattt ctttgattag ctgttgtact
    25321 gctttatttc tttcatcagc aatagaatgt agttctccac tatcacgatc aatacccaaa
    25381 actaattgcg ttaaatcatt tgaaccaata gacataccat caaaaatatc caaaaattca
    25441 tctgccaaaa ttatattaga tggtatctca cacataacat acactcttaa agacgtggac
    25501 aaatgttgat tttgactcgc taaacttttt tctttgtaac aatctcaatg tgaacaattc
    25561 ggatcggtgt taatactgat agatgctgat tgacgtttca agccacactc ttccataatt
    25621 tctataactt ttctaccttc ttggggagta cgacaaaatg gaatcatagg tataacatta
    25681 atcaatccaa aatcatcacg gactttttta agggctatac attccaactt aaatgcttca
    25741 ataaatcttg gatcataata acgagatgca ccacgccaac ctatcatagg attttcttcg
    25801 tgtggttcat ataattctcc gccaattaaa ctacgatatt cattggtttt aaaatcagaa
    25861 aaacgaacta taacatcttt gggataaaaa gcagcaccta ttttagcaat gccacgacac
    25921 aaactattaa tataataatc tgttttattt ttataacctt tggttaattt atcaatttta
    25981 gctcgcaaag cgggagttaa ttttttataa ttaatcaaag cattcggatg aattttgata
    26041 tcattaacaa taataaattc ttctcttgct aaacctacac catcattagg taaaaatcgt
    26101 aaactaaaag cctgattagg ttctcctaaa ttcatcataa tttttacatc aggcttttta
    26161 attttagata tatcaatctt tttaatttca aaatcaacaa atccatcata aatttttcct
    26221 tcttcccctt ctgcacatga aacagtaatt ttgtctccat tttttaaaat tttagtaata
    26281 tcttttacac ccacaattgt cggcactcca agttctctac caacaattgc tgaatgacat
    26341 gtacgactac cactttcagt aacaatagca ctggctaatt tcatagctgg tactcaatca
    26401 ggatcagtag cataagtaac taaaatttct cctttttgaa attcagcaag atgtttaaca
    26461 tctttaataa tttttacttt accctgagca attttagaac caatagacaa accagtagca
    26521 agcaatttag tgtttttgtt ttttctaatt tcatactgtt ctaaaatatc cacatccttt
    26581 ttactttgaa cagtttctgg tctggcttgc accatataaa tcttaccatc taatccatct
    26641 ttggcaaact ccatatccat agatttacca taatgatatt ctactatcat agaatatctg
    26701 gccaattcta aaatatcatc atcatcgaga gcaaaattat tttgctcttc ttttgtagta
    26761 tttatatttt tagtactgct cccttgttta ttagaataaa ttaatttttt ctcttttcta
    26821 ccaaggcgtt tttccaaaat tggtttaaac ccctgagcca atgttttttc ataaacataa
    26881 tactgatcag cttctacccg tcctttaaca atattttctc ctaatccata gctagcattt
    26941 attaatacta catcaccaaa acccgtttct gtatcacaag aaaacacaac tcctgatact
    27001 gctaaatcac tacgaaccat tttttgaata ccaactgata aacctatttc taaatgatca
    27061 aatccttttt ctaccctata agatattgcc cgatctgtaa ataaagaaat ataacaattc
    27121 ttaacagcag ttaaaagtgc tttatcacca taaatattca aaaatgattc atgggcacca
    27181 gcaaaagaag catctggcat atcttcggct gtagccgatg aacgtacagc aactggtaaa
    27241 ttattcgttt tgtattcttg acttaattta tgataatatt ctataatttc atgctctaaa
    27301 gctttgggta aaggagtttg tttaattaat tttctgatag ctttaccacg agtttgaaga
    27361 ccagcaatat ctcctaattt tcaatcagct aaaatgtctt taattttttg atacaaacct
    27421 gtatttttga gataatcaaa ataagcagta ctagtaatag caaaaccatc ggggatattt
    27481 aatccgttct ttttaatggg agcatatcct ccttcatcac caaaatgttt attatcataa
    27541 ccagctcgtg ataaacaaac acttaatgct tgataaattt tatctccaat acttaatgcc
    27601 tcttgataat tatcggctgc tatatttatc ataaattctt gtgtactaac tttatcttgt
    27661 cccaaaactt cacccttgtt taacgcatgt aatcccccgt taaacacatt catcaaaaat
    27721 ctaatttttt cgttgttatt aattgctctg tcattaattg tagaaggata aacttttcgc
    27781 aaatattcat aaagttcaat attttgtacc tttgcggcaa ttttagctaa agccaaagaa
    27841 acaaccagtg ttgtattacc tcctcattgc tgtaaatttt tcattaaaat attatctatt
    27901 tcgttttggt taaaaatgtt tttaccaata agctgttttt caatatcttt gatatttttt
    27961 aaagcttcat caatattttt tgttatggct tctttggtac caacagattt acctgatggc
    28021 acagaagctc aatcaccaat aatattatta tcaataataa ttcttgccct taaagttgga
    28081 ttattacgtg aatctaaaat agtatcggct tcaatttgtt taataaaata ttttttatta
    28141 ttaaattggt tgatattttt gtttaaaatc attgatcggc cagtcatttc tttgggttta
    28201 gaaatatcta aaatatcaag aattgttggg gcaatatcag ctaaaatacc tttgtctcga
    28261 agttttacac cattaagttt attataaaaa tctaccaaaa taaatggaac ggggtttgtg
    28321 ctatgagctg tgagcgtaga aggatcgtct acatcaactt cttcggcatt accatgatca
    28381 gcaataatta taaaagtata gccgagttgt tttccttttt caattattct accaagatta
    28441 tgatcaactg cttgaatagc ttttgtagta gctgataaat taccggtatg accaaccata
    28501 tctggattgg caaaatttaa gacaaaaaaa tcacaatcag ccattttatc aagcaaagca
    28561 tcagtaactt caaaagacga catctctggc tttaaatcgt aagtatcaac tttgggagat
    28621 gatattaata ttctttgttc attaggaaaa atattttctt gtcctccgtt aaaaaaataa
    28681 gtaacatgag catatttctc tgtttcggct atacgtaatt gttttaagtt gttacgtgcc
    28741 aaaacttctc ctaatgtatt tggtatttta cactgattat acgcaacaaa taaattagga
    28801 tactgactaa atttttcatc ataatttgtt aaacaaattc acattagatt attaaatgtt
    28861 ttggtagcaa aattattgaa tgttttattc ataaaagcct cagtaatttg tctgggtcta
    28921 tctggacgaa aatcaaaaaa tattactaca tcattatctt gaataatacc attttcatca
    28981 atgagggttg gtttgataaa ttcatcagtg atattatttt tatatgaatg ttctaatgct
    29041 tcttttcaag ttctttcttt tataccttga ccagctgtat atagattata tgctttttga
    29101 gtacgttctc atcttttatc tcgatccata gcataataac gaccacataa agaaacaatt
    29161 ttaccaatac ctattgattg agttttattt tctacttttt gtaatcattc taacgcactt
    29221 tttggtgccg aatctctacc atcagtaata gcgtggatac aaacattaga atatttttct
    29281 attttagcca aatctaataa agcaaataaa tgtgccattg aggaatgtac tgtggcagga
    29341 gatattaatc ctattagatg aacacgcgat ttatgttttt tagctgtttt gaaagcgtga
    29401 agcaaaactg gatttttgaa aaattttcct gtttgaatat ctttgttaat caaagtaaca
    29461 tcttgttgca ctattctgcc tgcaccaata ttcaaatgtc ctacttcaga attacccatt
    29521 tctttgtcta ttagaccaac atctttacca gaagcagata aaatagcatg tgggtatgta
    29581 tttcataaat aatcaaaatt ttgcggttgc gcttgtttaa tagcgttacc ttttgttgtg
    29641 tcttttaatc ctcatccatc taaaataatt aaaccaattg gagttttttt acgttttatg
    29701 ttttctaaat tttctaaata ggctatcctt tcatatttga cacgacgaac atcttgggct
    29761 ggatccatgg ttgggctttg atgtggagca ccagatttta ttccataagc atttgtagca
    29821 taagcaatat cagcaatgct agtatcaaga gtttcgccag aacgatgtga aataattcat
    29881 tttcaattat tttgctttgc cagaactatt gcttccatag tggcagataa agtgcctacc
    29941 tgatttggtt taatcaaaac agcattggca atattgtttt tgataccctt ggcaataaat
    30001 tttttattgg taacaaaaat atcatcacct attaaaatag tattaaaacc tattgatttc
    30061 tgtaattttt gtcatccatc tcaatcatct tcggccatag catcttcaat agatataatc
    30121 ggaaattcat caattaactg tttgtaaaaa tttatcaaat cgtcgctagt atattctttg
    30181 ccacctaaat aatacttacc atttttatat aaagaagaag cagcaacatc caaagataac
    30241 gacacgtctt tgccaggagt ataaccagct tcttttatag cttctattaa aattttaaga
    30301 gcatcttttt cttgacaaat tttcctctca gataaatttt gaatcatttc tccgagtgag
    30361 gcatttttac cacccactaa tggcacatca gacatattaa attcccgaaa ccacattaaa
    30421 tttttagaca taataatttt ttaatttatt aaattgagtt agtgataaat aaatttttaa
    30481 ttttaagata tattaataat agcataattg ttaaaaattt acaaggtgat attgatttat
    30541 taaataaaat atgatataat aaatatacaa taaaaataaa tatagcgcgg ggtggagaaa
    30601 tggtatctcg caaggcccat aaccttgaga ttgttggttc aattccaacc cccgcaacac
    30661 ccagatacga ataatactaa aaataaatag caaaaagtta aatgtttaaa aaatttttct
    30721 ttttgccggc acctataaaa aattttttag tgtctactta acaaactttt tattcttcaa
    30781 agttttttaa aaaaggaaaa caaaaagaaa ctaaaatagg gccggcatag cttagtggta
    30841 aagcgagggc ctcataaacc ctagacataa gttcaattct tattgccggc actagaaatt
    30901 tccaattttc aactcctttg ctaattttaa taaaataaaa tactgagtat cctatatatc
    30961 ttttttcaat ctctcaactt atgtttagtt agtatatcta tatgaaataa atgagaacca
    31021 agctttctat tttcaacttc tattatttcg aattttgcac tttctatttt aaacttttat
    31081 tatctgcgga tgtagttcag cccggttaga acgcctgcct gtcacgcagg aggccgccg
//
LOCUS       DAOTJA010000002        21716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_2786_length_21716_cov_5.499792, whole genome shotgun sequence.
ACCESSION   DAOTJA010000002 DAOTJA010000000
VERSION     DAOTJA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 21716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21716
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_21716_cov_5.499792"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..394
                     /locus_tag="P5241_00245"
     CDS             194..394
                     /locus_tag="P5241_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32025.1"
                     /translation="MNKNNNNKDTIQKIKTDIADINKQLNKVDTILQQSPHNVKLGYD
                     VVDIKVLTKNLRNLNDFIDTLD"
     gene            701..1039
                     /locus_tag="P5241_00250"
     CDS             701..1039
                     /locus_tag="P5241_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32026.1"
                     /translation="MISVNISLIEKLLENINPIKFNIDKEDISVTSRNVNEFLSTLDN
                     DFKSNKQNQSQQKLDLFFQQLFINIGNTLDIKRFTTLFTNLIFQSLDNKDIQIYSTNS
                     SVEHYLSQCS"
     gene            1196..1696
                     /locus_tag="P5241_00255"
     CDS             1196..1696
                     /locus_tag="P5241_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32027.1"
                     /translation="MILQNNNPKTVRTKSYSYLRVYIPPNSEIVNLVSASQRGPISRS
                     AINYEKQGFKIDNVLESINIKTVYIEDQDAKTFLEENFVGIGSWIITSSQTQKTFSLN
                     YKPNIHIDLQNNPHYNLFLQKQSGINRSYTIEVLYPAQNNPGQYNSITQTLILDKDTL
                     LSFDLN"
     gene            1728..3350
                     /locus_tag="P5241_00260"
                     /pseudo
     CDS             1728..3350
                     /locus_tag="P5241_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
     gene            3379..4149
                     /locus_tag="P5241_00265"
     CDS             3379..4149
                     /locus_tag="P5241_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV32028.1"
                     /translation="METFIRNFAIIAHIDHGKSTLADRFLEITNTVALSKMNAQFLDK
                     MFLEQEKGITIKMHPCQMKYTYEHNEYTLNLIDTPGHVDFVYEVSRSLAAIEGAILLV
                     DATQGIQAQTLGNLYLAQKHNLTIIPVLNKIDLKPQDVDILKKEIQDITKISAQNILS
                     ISAKTGQGTEDLLKQIIKTIPAPIKDDNKPLRALIFDSRYDSFKGVLAYVRVFDGTIH
                     KNDDLYLIAQKLKAKTIDIGIFKPDLESKQELKSGEIG"
     gene            4177..5091
                     /locus_tag="P5241_00270"
     CDS             4177..5091
                     /locus_tag="P5241_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32029.1"
                     /translation="MVKVGDTITHWHQVQDNAVEIMPLPGYEEPQPMVFAGFYPDTND
                     DFENLQKALAQLKLNDAALFYEPESNEALGRGFRLGFLGILHYEIISLRLEREFNLNI
                     ITTTPTVPYKVTFTDNKVAIIKNPADFPSSSQIKFVEEPYIQLEILTPSKYLNNIISL
                     LATFRSEQVDLQTLSQDLLLINYKLPLSELLLDLYDKLKSSSQGFASMNYKIIGYEKT
                     DVCKLDVLLHHEKFESLSFITPNVNLYAKARKYAETLKNYLPRQIFPLEIQVAKEGDI
                     IARETLPAFKKDVTGYLYGGDRTRKMKL"
     gene            5357..5599
                     /locus_tag="P5241_00275"
                     /pseudo
     CDS             5357..5599
                     /locus_tag="P5241_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
     gene            5782..5852
                     /locus_tag="P5241_00280"
     tRNA            5782..5852
                     /locus_tag="P5241_00280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5814..5816,aa:Gly,seq:ccc)
     gene            5993..6394
                     /gene="ruvX"
                     /locus_tag="P5241_00285"
     CDS             5993..6394
                     /gene="ruvX"
                     /locus_tag="P5241_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRV32030.1"
                     /translation="MKLLGIDFGEKRIGLSIADDKTKIAFPFKTIYHKNNKQTLEEIK
                     LILDNEGITDIVIGIPLSFSFQETQESKKIQEFISKLKEKFQNRNIHEENEILTSKEA
                     ENLLGIKKNKQGLIDAVAAALILQTFLDKHK"
     gene            6394..6519
                     /locus_tag="P5241_00290"
     CDS             6394..6519
                     /locus_tag="P5241_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32031.1"
                     /translation="MFKDIIQKNVPLAPYTTYRIGGRAKYFINIDNKDILLEALL"
     gene            6538..6864
                     /locus_tag="P5241_00295"
                     /pseudo
     CDS             6538..6864
                     /locus_tag="P5241_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            6997..7359
                     /locus_tag="P5241_00300"
     CDS             6997..7359
                     /locus_tag="P5241_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32032.1"
                     /translation="MSEILNNRHKSQPYMFPNAGCVFKNVNLELFPPEIISKYNLVIK
                     QNQNCQEVPAGFLIEQCGLKGYQIGGAQVSEKHANFLINKNNAKSSDIYNLICYIKDV
                     VKNRFGVNLEEEIRLIGF"
     gene            7904..8311
                     /locus_tag="P5241_00305"
     CDS             7904..8311
                     /locus_tag="P5241_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRV32033.1"
                     /translation="MNININVKNYVLLPREQQLLDTKLAFLEKILHNSKGNPSLDIDI
                     EELSKITNNGERFKVSATFTFFDSVIHMEEMNHNLKTALDALFDDLKVQIVKRIKKYK
                     SIKGRTAYKTKYAFLNKFLFRHKQNNKYSDDIM"
     gene            8321..10744
                     /gene="secA"
                     /locus_tag="P5241_00310"
     CDS             8321..10744
                     /gene="secA"
                     /locus_tag="P5241_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRV32034.1"
                     /translation="MFNFFDTNKKFFIQNRPTIDKINSLETELENLSLEVLLDRSINL
                     KNKIQDNTISLDKALIEAFALVREVAKRTLGQRHFDVQLLGGIALAQGKITEMKTGEG
                     KTLAATLPTYLNALCGKGVHVVTVNDYLARRDTVWMGQIYYALGLSIGCITQNDSYIY
                     DPSYTKENSLKDQSELDKNRDLLASFKVFQEYLRPCSKKEAYNADITYGTSSEFGFDY
                     LRDNLVRDLKDIVQRGHYFAIIDEVDSILIDEARTPHIISQPDEESSKLYQDFALIAS
                     RLQENEDYEADYKNKTITLLESGLNKLEQILGKNIYDLEGIENVHHLEEALRAQTFFS
                     KDKDYIVKDGKVLIVDEFTGRILPDRRYSGGLHQALEAKEHVYVNPESRTIATITIQN
                     YFRMYEHICGMTGTALTSAEEFEKVYHLKTVAIPTNKPLIRVDLPDKIYRTQDGKYKA
                     IIRAIEEKNKLGQPVLVGTRSVEINEKISSLLKIKGIPHNVLNAKYHEQEGQIIAQAG
                     KKGAVTVATNMAGRGVDIILGGNPADPIEREEIVKMGGLFVLGTERHEARRIDNQLRG
                     RAGRQGDPGSTQFFVSLDDELIKIFAPKTIGVLMERFGFAEDEAIEHPMITKAIETAQ
                     TKIEGINLDIRQHILEYDDVLNKQRQAIYNKRKLILSINQEQDLDDFVQSLNIDRKII
                     QEKKEKFGVDQFINIVKQIALSAIDSLWMIHLEKMDQLRDSVTLQSYAQKDPLVEYKR
                     LSFESYQELLNRFNNYIVDFITKIDKEINVEVKPQTEVIGSHKKIGRNDPCPCGSGKK
                     YKHCCGKNL"
     gene            10774..10947
                     /locus_tag="P5241_00315"
     CDS             10774..10947
                     /locus_tag="P5241_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32035.1"
                     /translation="MSIQIKDNKEPLVDIKKYCPGIIIDIDKPRMKKEKTAYVRRTVA
                     KMLHKAQTYLPNG"
     gene            11080..11313
                     /locus_tag="P5241_00320"
     CDS             11080..11313
                     /locus_tag="P5241_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32036.1"
                     /translation="MAPWKEIDASGHMTGGALDLRLIDKKGRKIPMKCRKLTYQQNAL
                     PNPKLLPTYIRNNRKILFSAMEKAGFSNCSNEY"
     gene            11380..11469
                     /locus_tag="P5241_00325"
     CDS             11380..11469
                     /locus_tag="P5241_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRV32037.1"
                     /translation="MKDSNHFYDNDPCPCGSGKKYKHCCGKNL"
     gene            11509..11637
                     /locus_tag="P5241_00330"
     CDS             11509..11637
                     /locus_tag="P5241_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HRV32038.1"
                     /translation="MKIIHKKIWPKYFDVISSGKKNFELRLNDFKINEGDTLILEE"
     gene            11781..12482
                     /locus_tag="P5241_00335"
     CDS             11781..12482
                     /locus_tag="P5241_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRV32039.1"
                     /translation="MSVFTIQTEKFSGPLDLLLKVIEEKNLEITDINLSNITGEYLKY
                     LESQEITPNDLADFLIVASTLLLIKSKAILPTLNLSNQEEEEIIDLKDRLTLYKNFKD
                     KSLYLKDRILSKTYFFSHEPFLDTSIHFSPPEKLKIEDLQNAFLQVFDIYQQENIIYP
                     SQKVKILVNLKERIQQILQKLTKGKKYNFNDLVDKNDKIDIIVSFLAILHLAKEGVVE
                     LNQDTNFSDIKLNAL"
     gene            12614..13156
                     /gene="scpB"
                     /locus_tag="P5241_00340"
     CDS             12614..13156
                     /gene="scpB"
                     /locus_tag="P5241_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRV32040.1"
                     /translation="MENKYKIEAVLFASGVPISIEELSRVCELSKTETEQAINDLKLE
                     YQEKQNGLTILEKITSGKAMVQLVSNDKASIYIQKLNKQILEGDLSPAALETLAIIAY
                     KGPISRGEIDDIRGANSSYILKILMIRGLIERYQNPERKNSFLYEVSFDLLKTLGIDT
                     IKQLPDFEKLHNLTFKNAEI"
     gene            13269..13880
                     /locus_tag="P5241_00345"
                     /pseudo
     CDS             13269..13880
                     /locus_tag="P5241_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            14072..14287
                     /locus_tag="P5241_00350"
     CDS             14072..14287
                     /locus_tag="P5241_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32041.1"
                     /translation="MNKNIVIYQLVRVIIMGGLSALFAFIMAPLVLKFLKKHNMIKHI
                     RKDSNAPIFTAMHQKKEGTPTMAGILI"
     gene            14384..14542
                     /locus_tag="P5241_00355"
     CDS             14384..14542
                     /locus_tag="P5241_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32042.1"
                     /translation="MILGIMALAALVGMLDDLLGIYGIGPKGGGLSMKKRILIYILIG
                     LIGAIWFF"
     gene            14624..15034
                     /locus_tag="P5241_00360"
     CDS             14624..15034
                     /locus_tag="P5241_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32043.1"
                     /translation="MFTACAFSMNETDGIDGLSAGVSMIGLLAYVIIVFIQGRYNVAV
                     LLAVTLGALLAYLWHNIHPAEFFMGDTGVMTLGVLFGILAMYTNTPLFLPLLLLIPML
                     ESMSVIIQLIYKKTHHGKKLVLSTPFHHHFEALG"
     gene            15735..16439
                     /locus_tag="P5241_00365"
                     /pseudo
     CDS             15735..16439
                     /locus_tag="P5241_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
     gene            16450..17289
                     /locus_tag="P5241_00370"
     CDS             16450..17289
                     /locus_tag="P5241_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRV32044.1"
                     /translation="MEEFNHKLFSNNALMLLLSCLIVSIIFGLVSWGYFTITQATNLT
                     SKDRTMTLSGSGKITAIPDIATISFEIVNEGKSIQTITQDNNTKMTAVVNYLKQEGID
                     DKDIKTSQYNIDPVYEQKCSYNTPTSSGTITKQPDTQSIETTKTCSSQISFYRISQTI
                     NVKIRDFNKIDNIIGKVSELGINNISNLAFTIDDPELLENQAKIEAIQNIKKRAFQFS
                     NETGIKLGRIINISESNYYPEYQKTSIMLNTPNLADSTMPSPIEPGSLEINKTMSITY
                     EIK"
     gene            17350..18426
                     /locus_tag="P5241_00375"
                     /pseudo
     CDS             17350..18426
                     /locus_tag="P5241_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
     gene            18554..18808
                     /locus_tag="P5241_00380"
     CDS             18554..18808
                     /locus_tag="P5241_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32045.1"
                     /translation="MEHNIILESEYIKNGIPFKDRNLIFFDTEVSSLFFDNEITELGW
                     LVVNPQTLEIKQEKDIKIKIEHIENADPKSLELSHYSNDL"
     gene            18980..19135
                     /locus_tag="P5241_00385"
     CDS             18980..19135
                     /locus_tag="P5241_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32046.1"
                     /translation="MAYIKLKDKTEITKFSLTEICNYFNISRNIEHNALEDARAAFEV
                     YKKLVVL"
     gene            19665..19946
                     /locus_tag="P5241_00390"
     CDS             19665..19946
                     /locus_tag="P5241_00390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRV32047.1"
                     /translation="MNKIIIYTDGGARGNPGPAGIGVIINGKPYYEYIGCNTNNKAEY
                     QAVVFALKKTKAILGARRINFDIPIEIYLDSELVCNQLNGTFKITDESL"
     gene            19991..20093
                     /locus_tag="P5241_00395"
                     /pseudo
     CDS             19991..20093
                     /locus_tag="P5241_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20285..>21716
                     /locus_tag="P5241_00400"
     rRNA            20285..>21716
                     /locus_tag="P5241_00400"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         8044 a         2767 c         3356 g         7549 t
ORIGIN      
        1 ttataaataa taaaaataac ttgtttatat catttttatt aatatctttg taaagctaaa
       61 tagagtaaat ggtattttat ttccttcgga attaggagat ttattattgt gatatagtaa
      121 ttttgatgat aaaataatat ctttacgtga taattttgcc tatattgttc aagatggtat
      181 tgggttagct gttttgaata aaaacaataa taataaagac actatccaaa agataaaaac
      241 agatattgct gatataaata agcaattaaa taaggtagat acaatattac aacaatcgcc
      301 acataatgta aaactaggat atgacgttgt cgatattaag gtattaacaa aaaaccttcg
      361 taatttaaat gattttatag atacattgga ctgattattg ggtaatcaaa aaaccatgcg
      421 ccatttagtt atttttcaaa acccctataa cttatcagct actggtggag aatgagtttt
      481 gtatggtatt ttgaactgta aaaattcgct atgtcaattt gataaaatag attttatttc
      541 taacttaaat atagctatgt tagataaact tgttccccct gatgaattaa aatattctaa
      601 ttctgtatga gtgtttcaat attttaattg attttttaat tttaagacaa gcgctcaaac
      661 tgcattaaaa tattatcctc aacaagagaa atttgatgga ttgatatctg taaacatatc
      721 cttaatagaa aagttattag aaaatattaa tccaataaaa ttcaatatag acaaagaaga
      781 tatatctgta acttctcgta atgttaatga gtttttatct accttagata atgattttaa
      841 atcaaataaa caaaatcaaa gccagcaaaa gttagatttg ttcttccaac aattatttat
      901 taatattggt aatactcttg atatcaaaag atttactacg ttatttacaa atttaatatt
      961 ccaaagtctt gataataaag atattcagat atatagtacc aattcatcag tagaacatta
     1021 tttatcacaa tgttcatgag ctggtagttt tcctgatagt attgataaaa atactgatta
     1081 tataggtata gctcgggcca atactactga taccgatata gatcaaaata taaaagaaga
     1141 tataaaaatt aaaattacca taaatgagaa aggagagata acaaatgatg taaaattgat
     1201 tttacaaaat aataacccca aaactgttcg taccaaaagc tattcatacc ttagagtata
     1261 tattccacct aattctgaaa ttgttaattt agtatctgcc agtcagagag gtcctatttc
     1321 tcgatctgct ataaactatg aaaaacaagg cttcaaaata gataatgtat tagaatcgat
     1381 taatataaaa actgtttata tagaagatca agatgccaaa acgtttttgg aagaaaattt
     1441 tgtgggtata ggatcgtgga ttattacttc atctcaaact caaaaaacat tttcattaaa
     1501 ttataagcca aacatacata tagatttaca aaataatcct cattataatt tattcttaca
     1561 aaaacaatca ggtatcaatc gttcttatac tattgaggta ttatatcctg ctcaaaataa
     1621 tcctgggcaa tacaattcaa ttactcaaac tttgatttta gacaaagata cattattatc
     1681 tttcgaccta aactaaatat ttattttttg taaactgaaa atttaaaatg ttccatcaat
     1741 tcttaaacaa acaaactaat catatagaat caagtgcttt tgttttagct ttttttactt
     1801 ttataggtag ttttttgagt ataataagaa acgccttact ggctagtact tttggtgctt
     1861 ctaaaacatt ggatatttat tacgcagcct ttcgtattcc tgattttatt tttaatatat
     1921 ttattttagg cgctttggct gctggattta ttcctttatt tattaaatat caaaatattg
     1981 ggaaaaaaga atctaatgaa tttgctacct ctattatggt aagtatttca tttttgagtg
     2041 ccttatttgg tattgtattt gttatttttg ctgatataat tgtgaaatga ttatttccag
     2101 ggtttgatct ggctagttta aatattatag caaacttatc aagaattatg atgattcagc
     2161 ccattgtgtt aggattatca tctttgattg gtaatattct acaagttaat aatttatata
     2221 tgcctattgc cctagctcca ttattatata atgtaggtat tattattggt atagtatgat
     2281 tttatcctat atttggagtt tacggtttgg gatatggcgt tgtcttaggt gcttttctta
     2341 atttaataat taaatattga ccatttagaa aaattgattt tgttttttca ttgccccata
     2401 gtaaagtttt taaacaatat attaaagaat ttagtgtttt aactagtttt agggcattaa
     2461 gtattatcaa tttacaatta tttttatttg ttatttcaaa tgttgcatct tatttacaac
     2521 caggtagtct tggtattata aattttgctt taaatattca agacttccct cgttctgtat
     2581 ttgctatgtc atttgccgta gcatcttttc caatattatc gcgacaattt accaataacc
     2641 aaaagaaaga gtttacagaa acttataaaa aaactttaat tcaaattttg tcttttctta
     2701 ttcctatttt ttttgcattt ttaatatttc gtagccctat tgtacgtctt ttgcttggtt
     2761 atggaaaatt tgattgaact gcaaccaata ctacaatttc tatatttact attttggtgt
     2821 ttggttttat tgctcatgcc ttacaagact ttttgttaaa tacattattt gccgccaaca
     2881 atactatagc accatttgtg tcagggttag gtgcatttgg tgttggtagc ttattatctt
     2941 tttatttagg gcgggttttt ggattaaaag gattggcgat tagttatatt atagctgata
     3001 ttctttatgc cttatttttg tttattagta gtattaagta ttttgataaa acaattttaa
     3061 agggattgat acaaaaaata ggaagtattt ttttctgcgc tttaatagct agtatatttg
     3121 gttttggtgc atttaaactt ttggaacaca tatttataac ttcaaaagta atttatttga
     3181 ttttagacag ctttgttgct ggtataatta tgatagctgt atatttattt atgatgtctt
     3241 tgttcaaaat caaagaattt aatacagcaa ttgattttgt aaaacaaaaa ctctttgtga
     3301 gaaaatttaa tataatgtct aaccagacaa gtcacaataa taatgaataa aatatataat
     3361 ttattaaatt aaatagttat ggaaactttt attagaaatt ttgctattat tgctcatatt
     3421 gatcatggta aatctacatt agcagatcgt tttttagaaa taactaacac agttgctctc
     3481 tcaaaaatga acgctcaatt tttggataag atgtttttag aacaagaaaa gggtattacc
     3541 attaaaatgc atccttgtca aatgaaatat acatacgagc acaatgaata cacattgaat
     3601 ttgattgata ctcctggaca cgttgatttt gtgtatgaag tttctcgttc gctcgctgct
     3661 attgaaggtg ctatactctt agtagatgca acccaaggta ttcaagcgca aactctcggc
     3721 aatttatatt tagctcaaaa acacaattta acaattattc ctgtgttaaa taaaatagat
     3781 ttaaaaccac aggatgtcga tattcttaaa aaagaaattc aagatatcac aaaaatttct
     3841 gcacaaaata ttttatctat ttctgccaaa actggacaag gcacagaaga tcttttaaaa
     3901 cagattataa aaacaatccc agccccaata aaagatgata ataaacctct acgggctctt
     3961 atttttgact ctcgttatga ttcttttaag ggtgttttgg cttatgttag agtttttgat
     4021 gggacaattc ataaaaatga cgatctttat ttaatcgccc aaaagttaaa agcaaaaaca
     4081 attgatattg gtatttttaa acccgattta gaatctaaac aagaattaaa gtcaggtgaa
     4141 ataggttgaa tagcaacagg attaaaagat ccgaccttgg taaaagtagg tgatactatt
     4201 actcattggc atcaagtaca agataatgct gttgagataa tgcctttgcc tggatatgaa
     4261 gagccacagc caatggtatt tgctggtttt tatcctgata ccaatgatga ttttgaaaat
     4321 ttgcaaaaag cattagcgca attaaaatta aatgatgctg ctttatttta tgaacctgaa
     4381 tctaatgaag cactaggacg tggatttcgt ttggggtttt tgggaatttt acattatgaa
     4441 attatttctt tgcgtttgga aagagaattt aatttaaata ttattactac tactccaacc
     4501 gtaccttaca aagttacatt tactgataat aaagtagcta ttattaaaaa tcccgctgat
     4561 tttcccagtt catctcaaat taagtttgtt gaagaaccat atattcaatt agaaatttta
     4621 acacccagta aatatttaaa caatattatt agtttattag ctacttttcg ttcagagcaa
     4681 gttgatttac agacattatc tcaagatttg ttgctaatta attataaact gcctctgtcc
     4741 gaactcttat tagatttata tgacaaatta aaatcgtcaa gtcagggttt tgcctcaatg
     4801 aactataaaa ttataggcta tgaaaaaacg gatgtatgta aattagatgt attattacat
     4861 catgaaaaat ttgaaagtct atcatttatt actcccaatg taaatttata tgctaaggca
     4921 cgaaaatatg ccgaaacttt aaaaaactat ttacctagac aaatttttcc tttagaaatt
     4981 caagttgcca aagaaggaga tattatagcg cgtgaaactt tgcccgcatt taaaaaagat
     5041 gttactggtt atttgtatgg cggagataga actagaaaaa tgaaattgtg aaagaaacaa
     5101 caaaaaggca agaaaaagtt agcagaaaag ggcaagatag aattaaatcc ttctatttat
     5161 cgcaagattt ttcttaataa agaatagatg ttttttatta aatttttatt atttattaaa
     5221 tattatgaga aaaaaatcat tacattctaa atgaaaaatt atatttatca ttgttttgat
     5281 aattattatc gtatttttat tatataaaat tatatttgat aatactatta aaagatgaaa
     5341 atttcttaga cagcaaacaa ataatctgca aaaagaattg aattatctta aagaagaaaa
     5401 tactaaatta aaaaaatctg ctaatacaga atcaaatgaa gaatatttag aaaaagaatc
     5461 tcgtttatct ttgggtatgc aaaaagaagg agagactgtt gtagttttgg aacaaactac
     5521 aacaactaca acaacaaaac cagaacaaaa tattttaaac accaatatca ataaaataat
     5581 tgattttttt aaaaattgat tccactaatt attatatttt gttctttatt agatatttat
     5641 attagatatc ccgcacacta attccactaa ttattatatt ttgttcttta ttagatgttt
     5701 atattagata tcccgcacac tgattccact aattatttcc ttttgttctt tattagatat
     5761 ttatattaga tgtttatttt tgcgagtgta gtttaatggt agaatataac cttcccaagg
     5821 ttaggacggg ggttcgattc ccctcactcg ctctttttat aacttgtttt gttattccaa
     5881 ctatataaca tacaatcact ttttatatta tttttgttta atgtttactt tttaattatt
     5941 ttatgctata atttaatata acaagatcaa ataaatataa ataaaaagat ttatgaaatt
     6001 attaggtatt gattttggag aaaaacgaat agggttatct attgctgatg ataaaaccaa
     6061 aattgccttt cctttcaaaa ccatttatca caaaaataat aaacagactc tcgaagaaat
     6121 aaaattaatt ttagacaatg agggtattac tgatattgtc atagggatac ctttgagctt
     6181 ttcatttcaa gaaacacaag aatcaaaaaa aattcaagaa tttatttcta aattaaaaga
     6241 gaaatttcaa aatcgtaata ttcatgaaga aaatgagatt ttgacttcca aagaagcaga
     6301 aaatttatta ggtattaaaa aaaataaaca aggattaata gatgctgttg ctgctgcttt
     6361 aattttacaa acttttctag ataaacataa ataatgttta aagatataat acaaaaaaat
     6421 gttcccttag ccccgtatac tacttataga ataggtggga gggcaaaata ttttattaat
     6481 attgataaca aagatatttt gctagaagct ttgttatgag caaaacaaca gcaattagat
     6541 gtgcttgttt tgggtggtgg tagtaatatt ttaattagcg ataatggatt tgatggacta
     6601 gttattaaaa tatataatac cacaaaagac ataaaggaag ttgatagtca atgtttatgt
     6661 acatttggtg caggagtacc ttatatgtca gcactcaatt ttatgtttga tcacaatctt
     6721 tttaatcttt taaatcttat tggtattcct ggtagtgtag gtggggcagt atatgggaat
     6781 gccggggttt ctaataaaga aacaaaagat ggaatttttg aagtacgagg attagattat
     6841 tcagatttag gtctgcctgt gtgaaaaact tttaatcaac aagaatgcaa ttttgggtat
     6901 cgtgatagtt tgtttaaaca tagtccaatt attatttgag aagttacgtt tattgatata
     6961 ccccaacaac ttaacaaaac agatgttcaa caaaaaatct ccgagatttt aaataatcgt
     7021 cacaaaagtc aaccatatat gtttcctaat gctggttgtg tatttaaaaa tgtaaattta
     7081 gaattatttc cacctgaaat tatatctaaa tataatttag taataaaaca aaatcaaaac
     7141 tgtcaagaag tccctgccgg ctttttaata gagcagtgcg gattaaaagg atatcaaata
     7201 ggaggagctc aagtttcaga aaaacatgct aattttttaa taaataaaaa taatgccaaa
     7261 agttctgata tttacaactt aatttgttat attaaagatg ttgttaaaaa tcgttttggt
     7321 gttaatctcg aagaagaaat acgattgatt ggtttttaat atatgtttgt ctgttattat
     7381 ttattattac aatcgatttt ttatactaaa aaataaaaac aaattttttc tttcttctaa
     7441 aatagaaaaa aatgttaaaa ccacttgact ttttttaaaa aatcatgtaa aattatatta
     7501 acacattcaa aaataattat tataaaaata attcaaaaat tattttaacc ttacataaat
     7561 attcctccat tttattaagg gtcgatggag ataaaaaata gaaaataaaa ttttaaaaat
     7621 atatcatggc tgcaaagaaa aaagcaaaaa aagttgccaa gaaagctaca aagaaaggta
     7681 agaaaggtaa gaaataattt ttagttcctc actaaaatag ttttaagcct attttaagga
     7741 agctttctaa atggaaacac cccggatata tccgaggtgt ttttttatat aatattgatc
     7801 aaaattatta gatatattaa gatgttttgg aattgttttt gagtcttgtt ttctttttaa
     7861 aaatatgcta aacttttatt atataaaaca ataaaatatc aaaatgaata ttaatattaa
     7921 tgtcaaaaat tatgtcttat taccaagaga gcaacagctg cttgatacaa aacttgcttt
     7981 tttggaaaaa atattacaca actccaaagg taatccatcg ctcgatattg atattgaaga
     8041 attatcaaaa attactaaca acggcgagcg ttttaaagtg tcagctactt ttacgttttt
     8101 tgatagcgtt attcatatgg aagaaatgaa tcataatctt aaaacagctc ttgatgcttt
     8161 attcgacgat ttaaaagttc agattgttaa acgcattaaa aaatataaat ctataaaagg
     8221 acgtactgcc tataaaacta aatatgcttt tttaaataaa tttttgttta gacataaaca
     8281 aaacaataaa tattcagacg atattatgta aatttgcgtt atgtttaatt tttttgatac
     8341 aaataaaaaa ttttttattc aaaatagacc tacaattgat aaaattaata gtttagaaac
     8401 agaattagaa aatctttcac ttgaggtatt attagataga tctattaatt taaaaaataa
     8461 aatacaagac aatacaatat cattagacaa agcacttatt gaagcttttg ctttggttag
     8521 agaagttgct aaaagaactt tgggacaaag acattttgat gttcaactgt taggtggtat
     8581 tgctttagct caaggtaaaa ttaccgagat gaaaacaggt gaaggtaaaa ctttggctgc
     8641 tactttgcca acttatttaa atgctctttg tggtaaagga gttcatgtag ttactgttaa
     8701 tgattattta gctagacgtg atactgtctg gatggggcaa atttattatg ccttgggatt
     8761 aagtattgga tgtattactc aaaatgattc atatatttat gatcctagtt atactaaaga
     8821 aaattcattg aaagaccaat cagaattaga taaaaataga gatttattag caagctttaa
     8881 agtttttcaa gaatatttaa ggccttgctc taaaaaagaa gcctataatg ctgatattac
     8941 ttatggtaca agctctgagt ttggatttga ttatttacgg gataacttag taagagactt
     9001 gaaagatata gttcaaagag gtcattattt tgctataatt gatgaggttg atagtatttt
     9061 aattgatgaa gcgagaaccc ctcatattat ttctcaacca gatgaagaat cgtcaaaatt
     9121 atatcaagat tttgctttaa ttgcttcgcg attacaagaa aatgaagatt acgaagctga
     9181 ttataaaaac aaaaccatta cattattaga atctgggcta aataaattag aacaaatatt
     9241 aggtaaaaat atttatgatt tagaggggat agaaaatgtt catcatttag aagaagcttt
     9301 acgagcacaa actttttttt caaaagataa agattatatt gtcaaagatg gtaaggttct
     9361 tattgttgat gaatttacag gtcgtatttt gccagacaga cgatattctg gggggttaca
     9421 ccaagcgtta gaagccaaag agcatgttta tgttaatccc gagagcagaa ctattgctac
     9481 tataactatt caaaattatt ttaggatgta tgaacatatt tgtggtatga caggtactgc
     9541 tcttacctct gctgaagaat ttgaaaaagt atatcatcta aaaaccgtag ctatcccaac
     9601 taataaacct ttaattagag ttgatttgcc agataaaatt tatagaactc aagatggaaa
     9661 atataaagcc attatcagag ctattgaaga gaaaaataaa ttaggtcaac cagttttggt
     9721 tggtactaga tcagtagaaa taaatgaaaa aatttcctca ttattaaaaa taaaaggtat
     9781 accacataat gttttgaatg ctaaatatca tgaacaagag ggacaaataa ttgcgcaagc
     9841 cggcaagaag ggagctgtaa cagtggcaac caatatggct ggtcggggag ttgatattat
     9901 tttgggtggt aatcctgctg atccaattga acgagaagaa attgttaaaa tgggaggatt
     9961 atttgtatta ggtactgaaa gacatgaagc tcgaagaatt gataatcaat tacgtggtag
    10021 agcgggtaga caaggagatc ccggtagcac tcaatttttt gtttctttag atgatgaact
    10081 gataaaaatt tttgctccca aaactatcgg agttctaatg gaacgttttg gttttgctga
    10141 agatgaagcc attgaacacc ctatgattac taaagctatt gaaactgctc aaactaaaat
    10201 agagggtata aatttagata ttaggcaaca tattttagaa tatgatgacg tattaaataa
    10261 acaaagacag gcgatttata ataaacgcaa actaattctt agtattaatc aagagcaaga
    10321 tttagatgat tttgtacaat ccttaaatat agacagaaaa attattcaag aaaaaaagga
    10381 aaaatttggt gtagatcaat ttataaatat tgtcaaacaa attgctttat cagctattga
    10441 tagtttatgg atgattcatt tggaaaaaat ggatcaacta cgtgattcag ttactttgca
    10501 atcttatgcc caaaaagatc ctctagtaga atataaacga ctttcttttg aatcttatca
    10561 agaattatta aacagattta ataattatat tgtagatttt ataacaaaaa tagataaaga
    10621 aataaatgtt gaagtaaaac cgcaaactga agttattggg tctcacaaaa aaattggtcg
    10681 caacgacccc tgcccttgtg gtagtggtaa aaaatataaa cactgctgtg gtaaaaattt
    10741 gtaatatgat taggttaatt aaatgattgt aatatgtcta tacaaataaa agataataaa
    10801 gaaccattgg tagatataaa aaaatattgt cctggaataa taattgatat tgataagccg
    10861 agaatgaaaa aagaaaaaac tgcttatgtt cgtagaacag tagccaagat gttacataaa
    10921 gcccaaacat atttaccaaa cgggtgaaat tttattatcg gtgatgcttg aagacctgct
    10981 tatgtccaaa tcagtatatt ttatggattt ataaaaaggt ttacaaaaga acatcctgac
    11041 tggacaaaaa agcgcgttgt tcaagaagct aataaatttg tggcaccatg gaaagaaatt
    11101 gacgcctcag gccatatgac aggtggagca ttagatttac gtttaattga taaaaaagga
    11161 agaaaaattc caatgaaatg tcgcaaatta acttatcaac aaaatgcctt acctaatcca
    11221 aaattattac caacctatat tcgtaataat agaaaaattt tattttctgc tatggaaaaa
    11281 gccggatttt ctaattgttc taatgaatat tgacattggt catatggcga ttattgatga
    11341 gctaaacgct ataaaaaatc cattgcaatt tatggaataa ttaaggatag taatcatttt
    11401 tatgataacg atccttgtcc ctgtggtagt ggtaaaaaat acaaacactg ctgtggaaaa
    11461 aatttgtaat atgattaggt taattaaata cagcatttca ataaaataat gaaaataatt
    11521 cataaaaaaa tatggccaaa atattttgat gttatttcct cgggtaaaaa gaattttgaa
    11581 cttcgtttaa atgattttaa aataaatgaa ggagacaccc ttattttaga agaatgaaat
    11641 ccaaaaacaa aacaatatac tggaagaaaa ataaaaaaac aagtaactta tgtaacaaaa
    11701 tttaaaatag ataaattatt ttggccagaa gaagaaatta agaaaaaagg aattcaaatt
    11761 atttcaataa aataattaat atgagtgtat ttaccataca aacagaaaaa ttctcagggc
    11821 ccttggattt acttttaaag gttattgaag aaaaaaattt ggagattaca gatattaatc
    11881 tctcaaatat tactggtgaa tatttaaaat atttagaatc tcaagaaatt acccctaatg
    11941 atttagcaga ttttttaatt gttgcttcaa ctctgctttt aattaaatca aaagcaattt
    12001 tacctacttt aaatttatct aatcaagagg aagaagaaat aatagattta aaagatcgct
    12061 taactttgta taaaaatttt aaagataaaa gtttatattt aaaagataga attttatcta
    12121 agacttattt ttttagccat gaaccatttt tggacacatc aattcatttt tctccgccag
    12181 agaaattaaa aatagaagat ttacagaatg cctttttaca agtatttgat atatatcaac
    12241 aagaaaatat aatataccct tcacaaaaag taaaaatttt ggtaaactta aaagaaagaa
    12301 ttcaacaaat tcttcaaaaa ttaaccaaag gtaaaaaata taattttaat gatttggtcg
    12361 ataaaaatga taaaatcgat attattgtta gtttcttggc tattttacat ttagctaaag
    12421 aaggagtagt ggaactaaat caagatacaa atttttctga tatcaaacta aatgccctct
    12481 aaattcccaa tcattttaat ttttattata tttataacgc ccgatcttta attatttaag
    12541 aaatttaaga agtaaaattg acacaagaca aataataacc caataataat ttttaaatgc
    12601 taatctattt attatggaaa ataaatataa aattgaagca gttttgtttg cctctggagt
    12661 accaatttct attgaagaat taagccgtgt gtgtgaatta agtaaaactg aaacagaaca
    12721 agctataaat gatttaaaat tagaatacca agaaaaacaa aacgggctga caattttaga
    12781 aaaaataact tctggtaaag caatggtgca gctagttagt aatgataaag caagcatata
    12841 tattcaaaaa ttaaataaac aaattttaga aggggattta agccctgctg ctttagaaac
    12901 attagctatt attgcttata aagggccaat atcaagaggt gaaattgacg atatccgtgg
    12961 tgccaattct tcttatattt taaaaatttt aatgattcgt ggattaattg agcgctatca
    13021 aaatcctgag aggaaaaatt cctttttata tgaagtatct tttgatttat taaaaacact
    13081 tggtatagat acaataaaac aattacctga ttttgaaaaa ttacacaatt taacttttaa
    13141 aaatgcagaa atctaacttt atattttgat tattatttgt tttggttatt ctttttttag
    13201 ggttaggatt tattattcat caacgaaata tttctggtgc gcaattaggt gcacattatt
    13261 tgagttcttt gtttaaaagt gataaaaata gtcaaccaat agatattttt gatggtcaaa
    13321 ttagtgcaga ggttgctttg gtgggtaata ttaatactgg cgaaatatta tatagtcgta
    13381 acgccaatgt acatacattg cccgcttcta ttggcaagct tatagctgct atggtaataa
    13441 ttgataattt taatatgaat gatactatta ctataactca ggatatgctc aaccccaatt
    13501 gaggtacttt acacttttct ttaaacgaaa aatttaaagt tcaagactta ctaaatatgg
    13561 ggctagtaat gtcgtgtaat gatgccataa tggcattggc atattcaata ggagaggata
    13621 catttgttgg tttaatgaat caaaaaataa aagaaatcgg tttagaagat acaggatttt
    13681 ttaacccttt attttttgat tcagaaggta attttacaac agcacttgac ttatttcaag
    13741 tatcgcgcta tatttataat aaatatcctc agataggcaa tattactaga atgaaggctt
    13801 atacttttaa atctgaatct ggtattaccc atttagtatt accaacgaat atattgcttg
    13861 ataaaattcc tgaaatatga ggtgccaaaa ctggtgttac tccagaagca aaagagtgct
    13921 tactcgttat ttatgaattt caacaaaaca atcaaaaagt tccctatgtt actatagtat
    13981 taaaatcaga caatagattc aaagatacag aaattttata tcaatgatta aaaaataaaa
    14041 taaatacatt ttaattatta aaatatacaa tatgaataaa aacattgtta tttatcaatt
    14101 agttcgtgtt attattatgg gaggattatc tgctttattt gcttttatta tggccccatt
    14161 ggtactcaaa tttttgaaga aacataatat gattaaacat attcgtaaag attcaaatgc
    14221 tcctattttt acagctatgc atcaaaaaaa ggaaggtact cctactatgg caggtatttt
    14281 aatctgaggt acagtatttt tgtttgctat tgtattttga gtattatctt ttataaatcc
    14341 ttcatataat ttttttaatt ttgtatccag accagaaaca tttttgattt taggtattat
    14401 ggccttagct gctttggttg gtatgctcga tgatttattg ggtatttatg gtattggacc
    14461 taaagggggg ggattaagta tgaaaaaaag aatattgatt tatattttaa taggattaat
    14521 tggggcgatt tggttttttt gaaaattaga atgagatgta gtccttgttc cctttatagg
    14581 aattatgcat ttaggttgat tttatccttt attttttatt tttattttta ctgcctgtgc
    14641 tttttctatg aatgaaacag atggtataga cggattatca gctggagttt ctatgatagg
    14701 gcttttagct tatgtaatta ttgtttttat tcaagggcgt tataatgtag cagttttatt
    14761 agcagttact ttgggtgcgt tattggcgta tctttggcat aatattcatc cagcagaatt
    14821 ttttatggga gatacaggag ttatgacatt gggtgtattg tttggtattt tggcaatgta
    14881 tactaataca ccattgtttt tgccattgtt actattaatt cctatgttag aatctatgtc
    14941 tgtgattatt caattgattt ataaaaaaac tcatcatggt aagaaattag ttttgtcaac
    15001 tccttttcat catcattttg aagctttggg ttgaccagaa accaaagtaa ctatgagatt
    15061 ttgaataatt cagggattag gcgctacttt gggtgttttg ctctatttat taaatataat
    15121 gttaattaaa taaaatgtta tgccaaattg attaaaagtt ttattcacag ctgctttacc
    15181 tatttcagaa ttgcgaggag ctattccttt ggggttattt gtttttaaaa tgggatactg
    15241 agagactatt atgttgtcag taattggcaa tttattacca attttacctc tgatatattt
    15301 tttaaaatat ggacaaaaat gattgtctca acattctata ttatttcaaa aattttttaa
    15361 ttggtgattt aaaaagaccg aaaaggcttt taatggtaaa tatcagagtt gggggaaatt
    15421 aggtttaatt atatttgttg ctatcccttt accaatgaca ggtgcttgaa caggtgcgct
    15481 cgctagtgtt ttatttaata tcaaaccaaa agaagcattt tcctgtataa cacttggtgt
    15541 tttaatcgcc tgtgctattg ttgttagttt gtgtagttta ggatatttga taatttaata
    15601 taaacaaaat ttattataat ttaaataact ataattaaat aactatgaaa tttattattt
    15661 taaattgaaa attatttcca gaaaacgaaa ttaaggctat tgacttagtt tctttaattt
    15721 gtcagaacta tcaaatgctt gatcttagat tacaaaaaaa atacagaatt attgtttgcc
    15781 ccccttgtat atttattcct ccaatttata agtttttgag caccaataaa tatcaaaatg
    15841 ttttatattt aggtggacaa aatgtgtttt atcgtaatag aggagcgttt actggtacaa
    15901 tatctcctgt aatgttaaaa tcctataata ttaaatattg tatagtaggg cactctgaat
    15961 gtaagaccat ttttaaggaa acaaatattg atataaataa taaaataaaa tccttattaa
    16021 aacaaaaaat aaaacccatt atttgtgtgg gcgaaaataa aagaaagtta aaagaaacct
    16081 cgtcaaattt tagaataaaa aatgagatat ttggacaatt aaatgaatat ttaaaaggaa
    16141 ttcctattga agaagcccac aatctaatga ttgcttatga gccagtatga gctattagtc
    16201 aaaatactcc aacttattta cctaatcctc aagatacaaa aaatattatt cacaatatta
    16261 gattttgatt aagtacacgt tttaatggta tgattgctca aaatattccc attctttatg
    16321 gtggttctgt taatcataaa aatatggcac aatatttaga aatatctaat ggcattttgg
    16381 tcggcagtgc aagtgttcaa aaaacagaaa taacaaaatt aataaaaaat ttaatataaa
    16441 tattacaata tggaagaatt taatcataaa ttgtttagta ataatgcttt aatgctttta
    16501 ttatcatgtt taattgttag tataattttt ggacttgtgt cttggggcta ttttactatt
    16561 acccaagcta caaatcttac ttctaaagat agaacaatga ctttgagtgg tagtggtaaa
    16621 ataactgcta taccagatat tgctactatt agttttgaga ttgttaatga gggcaaaagc
    16681 atacaaacta ttactcaaga taacaatacc aaaatgactg cagttgttaa ttatttaaaa
    16741 caagagggta ttgatgacaa agatatcaaa accagtcaat ataatattga tcctgtttat
    16801 gaacaaaagt gtagctataa tacaccaacg tcatcaggca ccattaccaa gcaacctgat
    16861 acccaaagta tagaaactac caaaacatgt tcttctcaaa tttcttttta tagaatctca
    16921 caaactatta atgttaaaat tcgtgacttt aataaaattg ataacattat aggcaaagta
    16981 tcagaattag gtatcaataa tatatctaat ttggcattta ctattgatga tccagaatta
    17041 ttagagaatc aggcaaaaat tgaggcaatt caaaatatta aaaaacgcgc ttttcaattt
    17101 tctaatgaaa ccggtattaa acttggtaga attataaata tttcagaatc aaactattat
    17161 cctgaatatc aaaaaaccag tataatgtta aacacaccaa acttagcaga ttctacaatg
    17221 ccatcaccca ttgaaccagg ctctttagaa ataaataaaa caatgtcaat tacttatgaa
    17281 attaaataaa tttcatataa taaatacata taataatata tttacattgc cataataaat
    17341 agaaataata tgttgagatt accaaaaccc aaagaaataa aaaataaaaa agtgttatta
    17401 aggattgata taaatgttga aaattttaat atacacaata ttcgttttca aaacattcag
    17461 ccgacaataa agtttttgtt aaaaaataaa gcaaaacaaa ttattataat tagccattta
    17521 ggaaggcctc gtcataaaga tccaaaatat tctttaaaaa agacattacc tgtcttagag
    17581 aaagttttta atcataaaat tgtattttat gataaattag ataatttacc ttctgataaa
    17641 attgttttgt tagagaatat tcgtttttga aaaggggaag aaaacaatga tatagacttt
    17701 gctaaaaaat tagctaaatt ggctgatatc tatataaacg aagctttttc tgtgtcgcac
    17761 cgtcagcatg cttctgtatc agctattact aaattattgc catcatatta tggttttgat
    17821 tttattagag aaattaaaca aattaatgaa tttaaaaaat ctatcaaaaa accagcatta
    17881 atgattttgg gcggagcaaa aataaaagat aaattacctt taatacaaaa atttaatcac
    17941 aaagtcaata atattattat cggtggcgcc ctggctaata ctttgcttga tatgttaggt
    18001 tttaatatcg ggagatcatt aactgacaaa aatattattc cgcaattacg taatctaaat
    18061 tttgccaaaa ttattttacc atttgatttt tgagttttag ataaaaataa tacaaaacag
    18121 catcgttttg tttttgagat acaaccatct gataacattt tagatgttgg taattattct
    18181 ttagaatatt ttaatgattt tgtttataga tctaaaacaa ttttttgaaa tggtccgatg
    18241 ggttatattg aagataaaca attccaacaa ggtaccgtag gtcttgttaa tttattaaaa
    18301 gtcagtaagg caaaaatatt agtcggggga ggggaaactt tgaattttac taaaccaata
    18361 gaacataaac aaaatgtttt tgtttccaca gggggcggtg ctttgttagc cgaactaatt
    18421 aaataaaaag tgatactaaa aataataatt tttcacttta aatttttaat aaaagtcaga
    18481 caatcaaaat gtaaattgaa taaatgtata aaagaataga aaatggtata tgaaaaattg
    18541 ataaataatc attatggagc acaatattat tttagaaagt gaatatatta aaaacggtat
    18601 tccgtttaaa gatagaaact taatattttt tgataccgaa gtttcgagtt tattttttga
    18661 taatgagatt acagaattgg gttggctggt agtaaatccc caaacattag aaattaaaca
    18721 agaaaaagat attaaaatta aaatagaaca catagaaaac gctgacccca aatcattaga
    18781 actatcacat tatagcaatg acttatgaac agatgctatt tctttatctg gggcactgga
    18841 aatgttccaa caaaattgta aaggtggagt attggtaggt tacaatgtgg cttatgattg
    18901 agagtgaata gaaagagcct tttataaagt aggaatattt ccaccgttat ttcattatca
    18961 taaattagat gttttatcga tggcttatat taaactaaaa gataaaacag aaattacaaa
    19021 gtttagtttg acagaaattt gtaattattt taatatctct cgaaacatag aacacaatgc
    19081 tttagaagac gccagagctg cctttgaagt atataaaaaa cttgtagttt tgtaagaaga
    19141 tgtgattgta taaaaattat gaactttata aaatttttta acatacaatt taatatataa
    19201 tatgaacaat atagccaatt ttatttcatt agtttttgta cattttatta tggattggct
    19261 ttttcaaacc aattatcagg cgttaaataa gagtaaacat tttaatgttc ttttgacaca
    19321 ttccactatt tatacccttg ggtttatatt tcctttttta ttgtttcata ttaatttatt
    19381 atgattatta ccaatatgaa tctggcatat aattcttgat aaaaggagtt ttttaatata
    19441 tctagcagaa catatcaaaa aaatgacaac ccaaaatact ccaagtgatc tttataatct
    19501 tattattata accttagatc aaatatttca cttattgtta ttatgaatag tagttttaat
    19561 agaatgttaa actatatacc acggataaaa taattattta tgcagatgga gtaagatcta
    19621 tttattttat actatatata atatacaaaa tataaaatta acatatgaat aaaattataa
    19681 tttatactga tggaggggct cgtggtaatc ctggaccagc aggtatagga gtgattataa
    19741 atggtaagcc atattatgaa tatatcggtt gtaatacaaa taataaagca gaatatcaag
    19801 ctgttgtttt tgcgcttaaa aaaacaaaag ctatattagg cgctcgtaga ataaattttg
    19861 atatacccat agaaatatat ttagattcag aattagtgtg taatcaatta aatgggactt
    19921 ttaaaataac agacgaaagc ttatgaccat actttatgaa tgtttgaaat ttaaggcgag
    19981 atttaaaaaa tgtttcgttt catcttatac ctcgagaaca aaataaagag gcagataaat
    20041 tagtaaataa agccttagac gaacacctta gtattgcaac ttcgctattt tagcgcgtgt
    20101 cttgtaaaat atattttaaa ctatataaaa aattcaaaga ccttgatttt tttataaaaa
    20161 tatgatatat ttaataatca gtagcactct tatatagaaa gatatttaga gtgtgaactt
    20221 taatatacta taatacaaaa caaccagttt ttaatttgga ttttttagtt gtctcaaatc
    20281 tattttcaat gaggatttga tcctagtcca gagtgaacgc tggcggcgtg gataaggcat
    20341 acaagtcgaa cgaacagaag caggagatca aaggttagaa gtaaataaac ttattatttt
    20401 aaacaatttg gtaattcaat attaagtaag agttattatt tagaataata aaaagcgatt
    20461 gataaatagt tatatttttt aataaaaaac gaaaataatt tagcttccaa tttttgatct
    20521 cttacttctg ttagtggcaa acgggttagg aatatcttgg tacgtacctt aaagtcagac
    20581 atagctcgtc gaaagtcggg gtaatttctg ataataccgt aaggttaaag ccgtaaggcg
    20641 ctttaagagc ggcctagatc ccatcagcta gttggtaagg taatggctta ccaaggcaat
    20701 gacgggtagg ggatgtgaga gcatgatccc caacagggac actgagacac gggtcccact
    20761 cctacgggag gcagtagccg agaatcttgg tcaatgggcg caagcctgaa ccagcgacgc
    20821 cgcgtgtagg aagaagttct tcggatcgta aactactttt atagaggaaa aatttaattc
    20881 ttttggatta aatgattgta ctctgtgaat aaggagctgc aaactacgtg ccagcagccg
    20941 cggtgatacg taggctccaa gcgttactcg gatttattgg gcgtaaagtg tccataggtg
    21001 gttttgtctc gcaagagagt ttttatattt gggtttactt gaatataaaa atctagtcag
    21061 ttattaaatt tcctggctca actaggaaca agtaattgat acggcaatac tagagttaag
    21121 aagaggttga cggaacgcac ggtgtagggg tgaaatccgt tgatatcgtg tggaacacca
    21181 aaagcgaagg cagtcaacta tttttaactg acactccatg gacgaaagcg tggggagcaa
    21241 acaggattag ataccctggt agtccacgct gtaaacgttg ggtgctggct atttgaagtg
    21301 tcgacccttc aagtggcgaa gctaacgcgt taagcacccc gcctgggtag tacagccgca
    21361 aggccgaaac tcaaaggaat agaccggagc tcgcacaagc ggtggagcat gaggtttaat
    21421 tcgaaggcac gcgatgaacc ttaccagggt ttgaaatgtt aatgaaagtc tattgaaaga
    21481 tagaccctcc gcaaggacat tagcacaggt gctgcatggt tgtcgtcagc tcgtggtgtg
    21541 aattgttccc ttaagtgggg taacgagcgc aacccttatc ttgtgttaca agtgtcacaa
    21601 gagaccgccc cctcctttgc ctaataggta aagaaacttt aaacatttaa cagttaaata
    21661 cattttaagt tactaatttt aattgttaat tttgttaatt gtttctttaa aaccta
//
LOCUS       DAOTJA010000003        18070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_3513_length_18070_cov_5.546878, whole genome shotgun sequence.
ACCESSION   DAOTJA010000003 DAOTJA010000000
VERSION     DAOTJA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18070
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_18070_cov_5.546878"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1312)
                     /locus_tag="P5241_00405"
     CDS             complement(299..1312)
                     /locus_tag="P5241_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV32048.1"
                     /translation="MFCQPEYQIIPKIVNKCIKSVVDIFILKPVETDEKTNDKNNILF
                     ETGENYIKISNSSGFFIDKSGILVTNYHSLIAKDLVYKVHYDNKYLDCEKIYGDEASD
                     IVLLKIINTQTNYPALKLGDSNDIQLGQVVIAIGNVLDEFEQTVSRGIISGLSRYVTM
                     SSANVEPQEYKGLIQTDAAINPGNSGGPLINLKGEVIGVNTLSVQGAENIGLAIPINP
                     IKKLLNDIKKFGKVRNVSLGIKYILIDNKIQLLHKLPVDYGAFIIYGSSNMYNVPGVL
                     KGSLADKNGIQEGDIILECNHKKITKEYNIQDILQNYQIGDSVIFKILRDDKTLDIKI
                     TLE"
     gene            complement(1460..1819)
                     /locus_tag="P5241_00410"
     CDS             complement(1460..1819)
                     /locus_tag="P5241_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32049.1"
                     /translation="MNFEEIQHNPNENKYLELRAEIQKARDIQYERFKNEPRKISLNS
                     EMGNTEIEKYCILDTKCNELLKQAMEKYALTMRSYHRILKIARTIADLNNQENIETQN
                     IQEAIQYKIGIENNDIH"
     gene            complement(1817..3022)
                     /locus_tag="P5241_00415"
                     /pseudo
     CDS             complement(1817..3022)
                     /locus_tag="P5241_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(3090..3161)
                     /locus_tag="P5241_00420"
     tRNA            complement(3090..3161)
                     /locus_tag="P5241_00420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3127..3129),aa:Gly,seq:gcc)
     gene            complement(3461..5002)
                     /locus_tag="P5241_00425"
     CDS             complement(3461..5002)
                     /locus_tag="P5241_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV32050.1"
                     /translation="MKINERKINGIVYTPKWIVNLILDEVGYKNDIYGKRIIDPACGN
                     GAFLIEVLKRFLTDVKKTNLSQEEIKDMLEKNIIGFDIDNQAINECISNLNNVALKYN
                     LKNIKWQIIKTDSLNKSFVSQYFNSFDFVVGNPPYIRIQHLGRERRKNIQNDWYCCKN
                     GSTDIFITFFELGFSLLNKNGQLGYITPNTYLKTNAGESLREFIKISKSLKTLIDFEY
                     YQLFKNTTTYSIITILDKNHHKNTFSLYKSDGKNINYIDDININNLDKRNWVLAPNNV
                     LDKLKKIETRGMPLNKIAKIHVGITTLADDFYIFKDIRFNNNYAEIKLKDGRTFQIEK
                     DILKPIIKVSVLKNANENQNRFIIFPYKKINGRHVIIPENELKSRFPLTYKYFLAIKK
                     NLDSRDKGKPNPITWYAYGRSQGLDTSFGKKILTSSINLKPNFIVWEKEEYTFYAGYC
                     IKFDGDLKLLAKYLNSPDMEFYINHTSRNYRNNYKSFAKSFIERFGITNLDLIKTQTL
                     FENKI"
     gene            complement(5008..5691)
                     /locus_tag="P5241_00430"
     CDS             complement(5008..5691)
                     /locus_tag="P5241_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32051.1"
                     /translation="MENKIITTSFLKAISAAFLAYNKFGARSNKKLLPIHKWFAEIIE
                     SKLTKDYSVRSLGKGGEVDLNGKYYPKTLDITILKGKKIITTISFKFVTSNYKQNSNN
                     YFENLLGETANIRRTGIGFAHFLVLRARTPYYDKNKGNLRGKEKKLEILHESDLLKYI
                     KLFKDLDFPHKPEVLGIAIIDFDRRGNAYLKNLDELKLSNKTKNILQNQLSLAEFIKK
                     VVALCKFKS"
     gene            complement(5741..5884)
                     /locus_tag="P5241_00435"
     CDS             complement(5741..5884)
                     /locus_tag="P5241_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV32052.1"
                     /translation="MKLHTNYFEFYTTKELAEKLRVNIMTIYRYIKAEKLKADKIDKG
                     VRL"
     gene            6110..6508
                     /locus_tag="P5241_00440"
     CDS             6110..6508
                     /locus_tag="P5241_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRV32053.1"
                     /translation="MSPQDIQKFQAKLLSEKKNIESQLSEFAIKDNTKSYDFNTKREN
                     LGFSPEDDALEEENFLRNIVIEQALEQRLKEIMESLAKIDNQTFGICERCHKPIEEAK
                     LEVNPASDFCIACNKIVSKQQKNIKTQAEY"
     gene            complement(6505..6759)
                     /locus_tag="P5241_00445"
     CDS             complement(6505..6759)
                     /locus_tag="P5241_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32054.1"
                     /translation="MNLMQALQQIPIKYRIKMAEAIDAVSQKLSDEFFNSLPKEKLLE
                     FNAINKNDKKALRQFVVDNFPDLNKTIMEEIVKYAKGERN"
     gene            complement(7260..7394)
                     /locus_tag="P5241_00450"
     CDS             complement(7260..7394)
                     /locus_tag="P5241_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32055.1"
                     /translation="MGRLENQQQKIKDKQAENKLKRLVVEGAILFGINTLGNLFIDNY
                     "
     gene            complement(7848..8693)
                     /locus_tag="P5241_00455"
     CDS             complement(7848..8693)
                     /locus_tag="P5241_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32056.1"
                     /translation="MKHFNKENINNNPQKAKQKNKQNPDTQHTYKTNKETDNKTKEKS
                     KSKKPLEQETQNGIELPLLNNIIAKQEADQYWSKLNPKLKNHNQEILNHNIEKMIKDG
                     KFEFVIYTNIKKPNESILTPDNKNIKKKFLFPLFIYQDKLLELGYTFASLVYYDKPKP
                     TIVAKIQPIETSRKKSEKTTHEQTEQENNKEKTDTIPIKTKLLKQVQDDLLKLATNED
                     IDLNEYPGLNYIQTLPLSNTDTIHIKLEDFDNFDQDMIDIFNPNPDEQQEPPDINNYR
                     GLKKL"
     gene            complement(9130..9339)
                     /locus_tag="P5241_00460"
     CDS             complement(9130..9339)
                     /locus_tag="P5241_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32057.1"
                     /translation="MFDFEKGIKHLQMFGAKYFLAYTSDAKNLADQYLIKLNQFEHFG
                     VYEIPNSKTIELIKDGFTLQYKHKK"
     gene            9914..10291
                     /locus_tag="P5241_00465"
     CDS             9914..10291
                     /locus_tag="P5241_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32058.1"
                     /translation="MPKPAKIQKTIKEFGNISSNLIIFVQCIILVYDLKAKKGNDHQA
                     VRQNAPPNTFTKYQNLYLYSSLLCCPINIKKGAVSTATITGSGWLRTINVRRRIFRIK
                     NFFSFNPRIINPKKIKNIAKEKE"
     gene            complement(10805..11095)
                     /locus_tag="P5241_00470"
     CDS             complement(10805..11095)
                     /locus_tag="P5241_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32059.1"
                     /translation="MCTKIILGVNFKDTQCGLKGFKTEIIQKISKIAISNRFAFEVET
                     MYLLQKMNIPIDILKVPPADYSHSTIKLKDIVSMLVDLLKIKYHKYNISKLS"
     gene            complement(11105..11506)
                     /locus_tag="P5241_00475"
     CDS             complement(11105..11506)
                     /locus_tag="P5241_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32060.1"
                     /translation="MAKVSIIIPAYQSEHCIDKTIEILIQNYITDIKNKEKEIIVVND
                     GSNDRTQEILEKYQKFITIIKNEKNMGRGFSVRRAYLSTNSEYALFTDADLPYGIDPI
                     NRLIEKLKQGSLAVVGQRDNIEHNLLRKITH"
     gene            11724..11891
                     /locus_tag="P5241_00480"
     CDS             11724..11891
                     /locus_tag="P5241_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32061.1"
                     /translation="MNNNKVCLIILDGWGIGVRDFTNPIFLAGTPYIDYLKQNYPYIA
                     LQASGLASGLD"
     gene            11961..12767
                     /locus_tag="P5241_00485"
     CDS             11961..12767
                     /locus_tag="P5241_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32062.1"
                     /translation="MKVDASIKDNSFFENKTLLRAINIAKAQNSSIHFVTMLSSGNNY
                     TKLRHLYVLLDILEQEKFYNAYFDIITDGLQSKNLQEALQLIEEFIEYLNKYKVGTIS
                     SVCGRYYALNKDSNKVYIDKYLSLLTQGQGTKTNIKDIALLLEPLYKKNMNDDFIEPI
                     AICDDNNNVHPIHNNDIVFMFNIQSKHKATSELINRLTENNFQIFSLVEYDNIPVENI
                     CFFEDTINNTLAQTLSNNHKRQLHIAEEIKQNHITNIFNGFHTDPFPGEY"
     gene            12774..13127
                     /locus_tag="P5241_00490"
     CDS             12774..13127
                     /locus_tag="P5241_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRV32063.1"
                     /translation="MPSVQSLKFIDHPEMMAHSITERASQAIEENIYDFILLNYANAD
                     QAGHSGNINIAKQVVKLLDEEIREIVEFALKQNYTILITSDHGNIEYMVDRLTGKPQT
                     AHDANLVPLHLVSNI"
     gene            13282..14583
                     /locus_tag="P5241_00495"
                     /pseudo
     CDS             13282..14583
                     /locus_tag="P5241_00495"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(14953..15189)
                     /locus_tag="P5241_00500"
     CDS             complement(14953..15189)
                     /locus_tag="P5241_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32064.1"
                     /translation="MKKFFVWLIIILFLLGSYGVYLVLIFSKQNTTPTTTVTTSVELS
                     DKILIFHSKNCPVCKELKKYVENYKIAEQLHNIQ"
     gene            complement(15354..15437)
                     /locus_tag="P5241_00505"
     tRNA            complement(15354..15437)
                     /locus_tag="P5241_00505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15401..15403),aa:Leu,seq:gag)
     gene            complement(15518..15964)
                     /locus_tag="P5241_00510"
     CDS             complement(15518..15964)
                     /locus_tag="P5241_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRV32065.1"
                     /translation="MDTNKLDEILRNKYKPNIPNVKVGDTVKIVLKLKEKTKNKDKNK
                     TQIIQGVVISHKHGKELGANITIRRILDGVGVEWTIPVYSSNIASFDIIKSPRVKKSK
                     LYYIRNKSVRETKTALKKEYQTKYKQQDITPATPTEQTTEKSEQQA"
     gene            complement(16090..16707)
                     /locus_tag="P5241_00515"
     CDS             complement(16090..16707)
                     /locus_tag="P5241_00515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRV32066.1"
                     /translation="MKIDIVFENNDFIVINKPANLTVHPLRISDTNTLVNGLLAYYPA
                     IRNVGDNILRPGIVHRLDKNTSGIMIIAKNNKTFQKLKTMFQKHLIQKTYIALIYGKM
                     PQKTGTINLAITRSKQKFNRRKIDLKNNNISHQAITRYKVIKEFKNTSLLEIYPETGK
                     THQIRVHFASLSHFIIGDKEYGSKKINKLFPIKRQFLHAQSLEFC"
     gene            complement(16744..16929)
                     /locus_tag="P5241_00520"
     CDS             complement(16744..16929)
                     /locus_tag="P5241_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRV32067.1"
                     /translation="MKIKIIATKQNRLDKTIHLQLYKLTRNKIEHFIQTQGVILNNKK
                     IYKPAYKVKTGDIILIN"
     gene            complement(17088..17161)
                     /locus_tag="P5241_00525"
     tRNA            complement(17088..17161)
                     /locus_tag="P5241_00525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17125..17127),aa:Val,seq:gac)
     gene            complement(17361..17729)
                     /locus_tag="P5241_00530"
     CDS             complement(17361..17729)
                     /locus_tag="P5241_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32068.1"
                     /translation="MNIFLFRLINNNRSQILDIISTIISSYIFIICIVGIIFLYVIFS
                     HKIIYKIKTISLIILAILLDVLIINIIAKNIVFTPRPYMVLPNVYTIGTSITKSAVPS
                     GHVSFISSILFALVISNPTL"
     gene            complement(17916..17990)
                     /locus_tag="P5241_00535"
     tRNA            complement(17916..17990)
                     /locus_tag="P5241_00535"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17956..17958),aa:Ile,seq:tat)
BASE COUNT         5868 a         2493 c         2524 g         7185 t
ORIGIN      
        1 actatacctc ctaatatagc taatataaca ataactatca ttagctctat tagagtgaaa
       61 ctgtatactg tgtcattatt gtttagtctt ttcttgttca tattacattt ttgatttact
      121 gtattcatat tgtgtgtgtt tttgatttgt tttgcttcta tcaatgtatt tgttaattta
      181 ttgtaattat atcataatat atggggggtt tgcaactatt ggcatagaga aagattagaa
      241 actcgagatt ataggttaaa aaagtaaaca ttataaacca aacatttaaa agtttatatt
      301 attctaatgt gatttttata tctaaagttt tgtcgtctct taatatcttg aatataacac
      361 tatcaccaat ttgataattt tgtaatatat cttggatatt atattcttta gtaatttttt
      421 tgtggttgca ttctaatata atatctcctt cttgtatgcc atttttatct gctaaacttc
      481 cttttaatac tcctggtaca ttatacatat ttgatgatcc ataaataata aatgcaccat
      541 aatctactgg tagtttatga agtagctgga ttttattatc aattagaata tattttatac
      601 ccaaagaaac atttctgact tttccaaact ttttaatatc atttaataat ttttttattg
      661 ggtttatagg tatagctagg cctatatttt ctgccccttg tacagaaagt gtattaacac
      721 ctattacttc gcctttaaga ttgattagtg gaccgccaga attgccagga tttattgccg
      781 catcagtttg aattaatcct ttatattcct gaggttcaac attagcagaa gacattgtga
      841 catatcttga taatcctgaa ataatacctc ttgaaacagt ttgctcaaat tcatctaata
      901 cattgccaat ggcaataact acttgaccaa gttgaatatc attagaatcg cctaatttca
      961 aagctgggta attagtttgg gtatttataa tttttagtaa aactatatcg cttgcttcgt
     1021 cgccatatat tttttcacaa tccaaatatt tattatcata atgtactttg tataccaaat
     1081 ctttggcaat aagtgaatga taattagtaa ccaaaatacc tgatttatca ataaaaaaac
     1141 ccgaggagtt acttattttt atataatttt ctcctgtttc aaataaaata ttgtttttat
     1201 catttgtttt ttcatcagtt tccacgggtt ttaatataaa aatatcaaca actgatttga
     1261 tacatttgtt tacaattttt gggataattt gatattctgg ttgacaaaac atagtatatt
     1321 agttaacaat atatagcttg tatataatga tagcaattat aataaatata gcacaaaaat
     1381 atctaaatgt aaacagcaaa taatatcttg ataaattatt aaatagtata taatttatgc
     1441 cccaactctt tgttgatgat tagtggatat cattattttc tataccaatt ttatattgta
     1501 ttgcttcttg gatattttgt gtttcaatat tttcttgatt atttaaatca gcaatggttc
     1561 gggcgatttt tagaattcta tggtatgagc gcatagtaag ggcatatttt tccatagctt
     1621 gttttaacaa ttcattacat tttgtatcca aaatacagta tttttctatt tcagtgttgc
     1681 ccatttcgct attcaaacta atttttcttg gttcattttt aaatcgctca tattgaatat
     1741 ctctggcttt ttgaatttct gcgcgtaatt ctaaatattt attttcattc gggttatgtt
     1801 gtatttcttc aaaatttatt ctatttacct tgacctgtaa atcaattctg tctagcaagg
     1861 ggccagaaat ttttctttta tatttcaaaa tagcaccagc gctacattcg cattctttta
     1921 gtttgtcacc taatcaacca caaggacaag ggttcatagc tgctaccaga ataaattttg
     1981 ctggatatgt aatagtgtcc tctgctctgg caacagttac tttgttttct tctaatggtt
     2041 gacgtagact ttcaagagtt tgtcgagaaa attcgggcaa ttcatctaaa aataaaactc
     2101 cattatgggc caaactaatt tctccaggtt tgggatgcgt acctccacct accaaagata
     2161 ccaatgacgc gctatgatgt ggggagcgaa aagggcgttt gtttaccaaa ggattttctt
     2221 ttgataataa cccagcattg ctgtaaattt tggtagtttc taaagtttct ctcaaagtca
     2281 ttgctggtaa aatataggaa aatgcttttg ataatagagt tttaccagat cctgggctgc
     2341 ctaccattag aatattatga ccacctgctg cagcaataat caaagctctt ttggctgatt
     2401 cttgtccctt aatttgagaa aaatctattt ctatattttc ttgatcgcga gtttgttgta
     2461 ataaattttg tacgtctata ttgggttctt gataattaaa attgccttct agataatcaa
     2521 ttacttctcg taaattattt aatccaacta cttttatatt ttgttttatt aacaaagctt
     2581 ctgtttcgtt ttctttgggc acaataattt catcaaaatg attattttga gccgcctctg
     2641 taaaagaaat aacactatta actggtttta aattaccttc taatgataac tcacctataa
     2701 gcattatttt tttatttgca attggtgtca tttgtccact ggctaacata taactaatag
     2761 caatagcgag atcataatta gaaccagttt ttttaatatt tgccggtgct aaatttacta
     2821 caactttttt acgagttcgt ataggagatt cagccccaag attttttaca gccgaaccaa
     2881 ttctttcacg agattctttg atagaactat cggcaagtcc taccacatta aaagcatgca
     2941 taccattaga aatgtcagtt tctacttcta taggtataga ttctaatcct ttactaaatg
     3001 ccgaaaatgt tttgccaacc atattatcag tttaaatttt ttaatgacct ttacctgata
     3061 ataatataaa ataatataat aatataaaga gcggcggacc gggcttgaac cggcgacctt
     3121 ctccttggca aggagacgtt ctaccactga actaccgccg cattatcatt taaaaaatta
     3181 aatctcaaaa acattattat aataatttgt aaattaacaa cttataaaat aaataatttg
     3241 tgtttagttt taaactaaat aaagattaac atatttttta gtaaaattca ataagtattg
     3301 ttcttgtgta aagtgtcagt atacccggaa gttatttttc tgtttgtgta cagctacatc
     3361 gttaacaatg taaaactata tcagcagtcg aatcagtata ttttatttct ttttattctt
     3421 tagttgaaac tctggctaaa attatagatg ccatagtttt ctaaatttta ttttcaaata
     3481 gggtttgtgt ctttattaaa tctaaattag taattccaaa tctttcaata aaacttttgg
     3541 cgaaagattt gtagttgttt ctataatttc tacttgtatg gtttatataa aattccatat
     3601 ctggcgagtt aagatatttt gctaaaagtt ttaagtcgcc atcaaattta atacaatatc
     3661 cggcataaaa agtatactct tctttttccc agacaataaa attgggcttc agattaatcg
     3721 atgaagtcag gatctttttt ccaaacgaag tatctaggcc ttgcgatctg ccatatgcat
     3781 accacgttat tggattaggt ttccctttat cacggctatc aagatttttt ttgattgcca
     3841 aaaagtattt ataggttaac ggaaatctgc tttttagttc attctctgga ataatgacat
     3901 gcctaccatt tatttttttg tatgggaaaa tgataaaccg attctgattt tcattagcat
     3961 tttttaaaac cgaaactttt attattggtt ttaaaatatc tttttctatc tggaatgttc
     4021 tcccatcttt taattttatt tcagcataat tattattaaa tcttatatct ttaaagatgt
     4081 aaaaatcatc tgcaagagta gttataccaa cgtgtatttt ggctatttta tttaatggca
     4141 tgcctcttgt ttcaattttc tttaacttat caagcacatt atttggggct aaaacccagt
     4201 ttcgtttatc cagattattt atgtttatgt catcaatata atttatgttt ttgccatcgc
     4261 ttttatagag agagaatgtg tttttgtgat gatttttatc aaggatagta attatagaat
     4321 aagtggttgt atttttaaac aattgataat actcaaagtc aattagagtt tttaacgatt
     4381 tacttatttt aataaactcc cttaaacttt ctcctgcatt tgtttttaaa taagtatttg
     4441 gtgtaatgta tcccaattgt ccatttttat ttaataaaga aaaaccaagt tcaaaaaaag
     4501 tgataaaaat atcagttgat ccatttttgc aacaatacca atcattttga atattttttc
     4561 ttctttcacg tccgaggtgt tgaattctga tgtatggtgg gtttccaaca acaaaatcaa
     4621 aagaattaaa gtattgactg acgaatgact tatttaaact atcagttttt attatttgcc
     4681 attttatatt ttttaagttg tattttaatg caacgttatt taagtttgaa atacattcat
     4741 tgattgcttg attatctata tcaaaaccaa tgatattttt ttctaacata tctttaattt
     4801 cctcttggct taaatttgtt tttttaacat cagtaagaaa cctttttaat acttctatta
     4861 aaaaggcacc attaccacaa gcagggtcaa ttattctctt gccgtaaata tcatttttat
     4921 agccaacctc gtctaaaatt agattgacaa tccatttcgg agtataaact atgccattta
     4981 tttttctctc atttattttc atatttttta tgatttaaat ttacataacg ctactacttt
     5041 ttttataaat tctgccaaag ataattgatt ctgtaaaata ttttttgtct tatttgatag
     5101 ttttaattca tctaaatttt ttaaataagc attccccctt cgatcaaaat ctataattgc
     5161 tatacctaaa acttctggtt tgtgaggaaa atccaaatct ttaaaaagtt taatatattt
     5221 taataagtcg ctttcatgaa gtatttccaa ttttttttcc ttgcccctta aattaccttt
     5281 gttcttatcg taataaggcg tacgagctct taaaacgagg aagtgagcaa agccaatacc
     5341 tgtccgtcta atatttgctg tttcgccgag tagattctca aaataattat ttgaattttg
     5401 tttatagttt gaagtaacaa atttaaaact tattgttgtt ataatttttt taccttttaa
     5461 aatagttatg tcaagtgttt tgggataata cttgccgttt aaatcaactt caccaccctt
     5521 tcccagactt ctaactgagt aatcctttgt taatttactt tcaataattt ccgcaaacca
     5581 tttatgaatt ggtaaaagct ttttattact tctcgcgcca aatttattat aagctaaaaa
     5641 cgctgcggaa atagctttta gaaatgaagt agttataatt ttattttcca tatttttatt
     5701 tagtttttgt tttatctata aaccttttaa actctttttc ttaaagccta acccctttgt
     5761 ctattttgtc ggcttttaat ttttctgctt taatatagcg gtaaattgtc ataatattta
     5821 ctcttaattt ttcggctaat tctttggttg tataaaattc aaaataattt gtgtgtagtt
     5881 tcaaactaaa taaagattaa catatttttg agtaaaattc aatagataac aataaacgcc
     5941 aagtattata gatgttaaga atacgtagat ttggttttta taacgagcac caagctctat
     6001 gtttggtagc ttctaatttt taaacttcta ttttctattg gggcttgcaa aaaaagtaaa
     6061 aatcaggtat acttggatta tattaagcaa cagactaaat ttaaatttta tgtctccaca
     6121 agatatacaa aaatttcaag cgaaattatt atcagaaaaa aagaacattg aaagtcaact
     6181 ctcagaattt gctatcaaag ataacacaaa atcttatgat tttaatacca aaagagaaaa
     6241 tctggggttc tcgcccgaag atgatgcttt ggaagaagaa aattttttac gtaatatagt
     6301 tattgagcaa gctttagaac agaggttaaa agaaattatg gaatctttgg ctaaaatcga
     6361 caatcaaaca tttggtattt gtgaaagatg tcataaacca atagaagaag caaagttaga
     6421 agtaaatcca gcttcggatt tttgtattgc ttgtaataaa atagtatcta aacaacagaa
     6481 aaatattaag acgcaagcag agtattaatt tcgttcgcct ttagcatatt ttacgatttc
     6541 ttccattata gttttgttga gatcaggaaa attatcaacg acaaattgcc ttaaagcttt
     6601 tttgtcgttt ttgtttattg cgttaaattc taataatttt tcttttggca aagaattaaa
     6661 gaattcatct gataattttt gagaaaccgc atctatggct tctgccattt ttattctata
     6721 ctttattggt atttgttgta aagcttgcat taaattcata tgtatttttt atatttttat
     6781 taaaatatat ttattatatt ttgattaaaa tatatttatt atatgttata ttttgattaa
     6841 aatatatttt ttatattttg attaaaatat ctgtttttgt ttttattttg ttgttgtgct
     6901 acctttttat cttgtttttt atttgtattt aatatgttgt ctaaattcgg cttgatgttt
     6961 tgtggaaatt agccttttgt tcttttgctg ccttttttag tttgtttttt cttgcttttt
     7021 agcgctttat ctaaattcgt tatagacatt agtttttatt ctatacttta ttggtatttg
     7081 ttgtaaagct tgcattaaat tcatatgtat tttttatatt ttgattaaaa tatattttat
     7141 tttttttgtg gtttgctatc tttttttggt ttactacttt tttctttact caatgtttta
     7201 cttaaatttg gtatagacat tggtgaggta gttgtgtctc atattcattt caaagctttt
     7261 caatagttat ctataaataa attaccaaga gtgttaatgc caaataatat tgctccttct
     7321 acaactaatc tttttaattt attttctgct tgtttatctt tgattttttg ttgttgattt
     7381 tctaacctcc ccactattgt ataggagtca tttttatcta aatagcagta tcaatctgta
     7441 atggtatagt tgtatttttg tgcttcaatt atataggcag ctatgcgatt taaaaattca
     7501 ttagtaaaac ccttatcagt aaaaatgtcg tcatgctcta atgaaaattc aaatttattg
     7561 ctattgttta tattatcaat cgcttttttg actgctgttt ctatttcttt tcgagattct
     7621 ggtgggtttt gttgtttgta gtattcattg ctagcctttg ataattgtgt ttcatctatt
     7681 tctattgtta tagcataggg gtgactatct cgttcgtttt ggtattgtgt ttttgggtca
     7741 ttttgtcccg tattgatatt ttcttcaaat tgtttttgcc aagcgcgtca tttgtcatta
     7801 taatcattat actcattatt ggtatgaaga cgatctttat atatatctca tagctttttt
     7861 agacctctat agttattaat atctggtggt tcttgttgtt cgtcaggatt gggattgaat
     7921 atgtctatca tatcttgatc gaaattgtcg aaatcctcta attttatatg aattgtatct
     7981 gtgttgctta gtggcaatgt ttgtatataa ttgagacctg gatactcatt caaatcaata
     8041 tcttcgtttg tagctaattt caataaatca tcttgtactt gttttaataa ttttgttttg
     8101 attggtatag tatcagtttt ttctttattg ttttcttgtt ctgtttgttc gtgcgtggtt
     8161 ttttctgatt tttttcttga tgtttctatt ggttgtattt ttgccacgat tgttggtttg
     8221 ggtttatcat aataaaccaa agaagcaaaa gtatacccca actctaataa tttatcttga
     8281 tatataaata aaggaaacaa aaatttcttt ttaatatttt tattatctgg tgttagaata
     8341 ctctcgttgg gctttttgat attagtatat attacaaatt caaatttgcc atcttttatc
     8401 attttttcta tattatgatt taatatctct tggttgtgat tttttaattt tggatttaat
     8461 ttactccaat attgatcagc ttcttgttta gctattatat tatttaaaag aggtaattct
     8521 atgccatttt gtgtttcttg ttctaggggt ttctttgatt tactcttttc ttttgttttg
     8581 ttgtcagttt ctttatttgt tttgtaggta tgttgggtat ctgggttttg tttatttttt
     8641 tgtttggctt tttgaggatt gttattgata ttttctttat taaaatgttt cattgttgtt
     8701 tttatttata aaatttttat ttaaacgttt gtgtttattt attactatta tacacaaaaa
     8761 tacaaaagaa gtcaaactca aaatataacc cgttcaatca ataatacctg ccttgaattc
     8821 taaggtaaca ttattttggt agggaaacac aaccataaaa gatggcgaaa ttagataggg
     8881 accataggct cccttgactg ttcaatctgg gaaatatgat attttaatta tatggggcct
     8941 ataaagatta tttgttgtaa attgtaattg cgtgtcagtt tgtttgataa tttgaatatt
     9001 ttcttcttgt aaaggttgta ttttaggaat tatttgttgt tcaataaaat ttttttcctt
     9061 aatattttta taaaaaagaa ttggtatatt taaatcgccc ttggtatatc aatcaaatga
     9121 atcatatact cattttttat gtttgtattg cagtgtaaac ccatctttta taagctcaat
     9181 tgttttggaa tttggtattt catatacccc aaaatgttca aattggttta attttataag
     9241 atactgatca gcgagatttt tggcgtcaga tgtataggct aaaaaatatt tagcaccaaa
     9301 catttgtaaa tgttttatgc ctttttcaaa atcaaacact ggataatcaa atccagcaat
     9361 agccgaagat ggccattttg atacttctgc ttgattaaca aaatggaatg gtcctgacag
     9421 agatgattca ataagtagcc cttcatacgt aggatggttt gttcaaattg gtaaattttc
     9481 taaaaatatt ggtgtaccat aagtatcata ttcttcgttt ttgcactctc acattatacc
     9541 tccttttggc agagttttta agtattggaa catagctatt gcttctgttt gaaaaggtat
     9601 tttggcttca aagccctcat aatttcattt taatcagtct ggtataaatg aaatattttg
     9661 ttttaccatt attcaagatc caatccccaa taatattaca attatactca ataaaaattt
     9721 tttatctaca ataagtttta gagttttgcc cagaccataa gctcctaata aaatataact
     9781 tacaattaca aatggcaaaa atctcgcatt caaaatagat gaattattga taaaataata
     9841 taataaaaaa cttacaccac ctgttcatat caaggctcat atttgttttt ctttatgagt
     9901 aatagcataa attatgccta aaccagcaaa gattcaaaaa actattaaag aatttggaaa
     9961 tatttcttct aatttgataa tttttgttca atgcattata ctggtatatg atctaaaagc
    10021 taagaaaggc aatgatcatc aggcagttag acaaaatgca ccgccaaata cttttactaa
    10081 ataccaaaat ttgtatttgt attcttcttt gttatgttgc cctataaaca tcaaaaaagg
    10141 cgcagtaagt acggctacaa ttactggcag tggatggctg agaaccatta atgtcagaag
    10201 aagaatattt agaattaaga attttttttc atttaatcct cgtattataa accccaaaaa
    10261 aataaaaaat atagcaaaag aaaaagaata ggaaaattct cctgctaatg tacttggtaa
    10321 gtttgctcca taaatagaaa atgtttctac aaaaatataa aacaaagcac ccaaagcacc
    10381 tatctcagga atgggaaatt tttgattgca attttttaaa catcaatata aagctattgg
    10441 taataaaaat gtacccaaca aagtagccag cttaaaagca atattcaggg gtattaaata
    10501 gcttcatata acagacaaaa taaacattaa tggtgggtaa aaatataaaa atggaaaccc
    10561 cgcatatcaa tcattggttc aagtttttat tgtggggaat atagatttta aatgatataa
    10621 agtataatta tgtgcccctg tgtctcctcc tgccaccatt gtatctttga aaattaaatt
    10681 tcaaggtaat atttttataa taaatacata aattattata aaaataacaa ttgttgctat
    10741 tgtttgccat ttaatatttt tgaattggtt attttttatc ataatttgag tttttagtat
    10801 ttatttaaga tagttttgat atattatatt tatgatattt aattttgagt aaatctacaa
    10861 gcatagaaac tatatctttt aattttatgg ttgaatggct ataatcagct ggtggcactt
    10921 tcaaaatatc aataggtata ttcatttttt gcaacaaata cattgtttca acttcaaaag
    10981 caaaacgatt gctaatagct attttgctaa ttttttgaat aatttctgtt ttaaatcctt
    11041 ttaaaccgca ttgagtatcc ttgaaattta ctcctaaaat aatttttgtg cacattaaaa
    11101 atcctcagtg tgttattttc cttaataaat tgtgttctat attgtctctt tgtcccacta
    11161 ctgccaaact gccttgtttt aatttttcaa tcaaacgatt tataggatca ataccatagg
    11221 gcaagtcggc atcagtaaat aaagcatatt cactatttgt tgataaataa gctctgcgta
    11281 cactaaatcc gcgtcccata tttttttcat ttttgataat ggtaataaat ttttgatatt
    11341 tttctaaaat ttcttgcgtt ctgtcatttg atccgtcatt aacgacaata atttcttttt
    11401 ctttgttttt aatatctgtg atataatttt ggattaatat ttctattgtt ttatcaatac
    11461 aatgttctga ttgataggca ggaataatta tagatacttt ggccataaaa taaatttata
    11521 gattatagta atctaattta ttagttattt gttgtaaaag tgtatagctt ttcttagtgg
    11581 tataattgtt ataatgttag agtgctatgt atttatttta tcatataatg tagaaaaatt
    11641 caatgatttg tgctatacta taaatagtag tataatattt aataataaat aatagtttaa
    11701 gtaactataa ataaactcat aatatgaaca ataataaggt ttgtttgatt attcttgatg
    11761 gttggggtat aggtgttcgt gattttacca atcctatttt tttagctgga acgccctata
    11821 ttgactattt aaaacagaat tatccgtata ttgctttaca agcttccggt cttgcttctg
    11881 ggttagattg aaaagaagaa ggcagcagtg agattggaca tttaattctt ggagctggtc
    11941 gaagtgttct cccagcagct ttgaaagtag atgcgagtat taaagataat agtttttttg
    12001 aaaataaaac acttttaaga gctattaaca tagccaaagc acaaaattcc tctattcatt
    12061 ttgtgactat gctttcgtct ggtaataatt atacaaaatt acgacatcta tatgttttat
    12121 tagatatatt agaacaagag aagttttata atgcttattt tgatattatt actgatggat
    12181 tacaaagtaa aaatttgcaa gaagctcttc agcttataga agaatttata gagtatttaa
    12241 ataaatataa agtagggaca atatcatcag tttgtggtcg ctattatgct ttgaacaaag
    12301 attctaataa agtatatata gacaaatatc tttctttact tacgcaaggt caagggacca
    12361 aaacaaacat caaagatatt gctttattgt tagaaccttt gtacaaaaag aatatgaatg
    12421 atgattttat tgagccaata gcaatttgtg atgataataa taacgtccac ccaatacaca
    12481 ataatgatat tgtttttatg tttaatatac aatcgaaaca taaagctaca agcgaattga
    12541 taaatagact tactgaaaat aattttcaga tatttagcct ggtagaatat gataatatac
    12601 ccgtagaaaa tatttgtttt tttgaggaca caataaataa tactttagcc caaaccttat
    12661 ctaataatca taaacgtcag ctacacattg ccgaggaaat aaagcaaaat catataacta
    12721 atatttttaa tgggtttcat acagatccat ttccaggaga atattgaaat attattccaa
    12781 gtgtacaatc tttaaaattt attgatcatc cagaaatgat ggcgcattct ataacagaaa
    12841 gagcctcgca ggccatagag gaaaatattt atgattttat tttattaaat tatgccaatg
    12901 ctgatcaggc tggtcatagt ggtaatatta atattgctaa acaagtagta aaattacttg
    12961 acgaagaaat acgagagatt gttgaatttg ccctaaaaca gaattataca attttaataa
    13021 catctgatca tggtaatata gaatacatgg tcgatcgatt aactggtaaa cctcaaactg
    13081 ctcacgatgc caatttagtg cccttacatc ttgtatctaa tatatgaaaa tttaatgttg
    13141 ctaaaattca agctgatata gataaaattg agcgcgaagc actggggctt ttgagtgatg
    13201 tggctccaac aattttagat attttggata tttctaaatc caaagaaatg accggtactt
    13261 ctctattaaa atatatacac aatgaataaa attattgagc aaaaaataca aaaatctcct
    13321 gaaagttgtg gcgtatataa attttttgat cagaataata aaattattta tattggcaag
    13381 gcatctaatt tgaaaaagcg tttgcgttct tatttaaata gcaataatta caaaaatagt
    13441 aatttaaaca aagaagccag aaatttaaca tatgagatta ctcacaatga ggctgaggct
    13501 ttaattttag aatctcgact tattaaaaaa tacgagccaa aatttaatgt tttacttaaa
    13561 gatagttcca gttatttttt tgtagaaata aataatgata ttttccccaa aatttttata
    13621 gttaggcaaa agaaaaaccc tatgtcatta tatattggtc cttttaccaa tggaactatt
    13681 ttaagaaata ttttggttgc gttgagaaaa atttttcctt attgtacatg taaacgtatg
    13741 cataaaggca gttgttttaa tgctaaaatc aatttatgcc caggatattg ctgtaataat
    13801 acaatatcat ttactaatta tcaacgtcgt gaatatttaa aaaatattaa aaatatccaa
    13861 aatattttgg aaggtaatat tgaaaaaatc aaacatactt ttgagcaaga aatcaaaaaa
    13921 ttagttgctc gtgaagaatt tgaacaagcc gccaaaattc gtgatactat tttggcctta
    13981 gataatattt ttgaacataa aacaattgtt tcgcacaata taggcaatgt acacaataat
    14041 tttttaatat taaaacaaaa tgaaattttg caatcattaa aaaagatttt tggtttgtct
    14101 aatatcccgc acagaataga aatgtatgat atttcgaata tttctggtga ttatgccgta
    14161 ggatcaatga ttgtttatca taataataaa tttgataaaa atgagtatcg tcgttttaag
    14221 attcgttata ccaatgagac ccccaatgat ccattaatgt taaaagaagt attattgcgt
    14281 cgcagtaaac aaaaatgaga acctccagat attttgttag ttgatggtgg taaagcgcaa
    14341 tttaatattg caaaacaaat tattcaaaat aataaaaatt tcaaaaatac taaaatagga
    14401 gcacttgcca aaggtaaaca aaacttattt atagataatt ctttaaagcc tattgcccta
    14461 aaacaattac ccttagaaac aaaattattt ttaatgtctt tacaagatga agcccatcgt
    14521 tttgctgtgt catattatca tattgtatat agaaaacgca atttagaagg taaggtatat
    14581 taaatatatt ccaatacgga tctatagatt gttattatag atttgccata taaactataa
    14641 aactacaaag gctttttata aaaagccttt gataatatta taataattat acgcttgtaa
    14701 atgtaaattt tcttattatt tgttaacagg tataactgtg ctcgatgtgg tagttgtgcc
    14761 tattgtagta gaacttgagg cacttattaa atcatttatt aatattgtga tttggtcact
    14821 accaatatag catttaccat tataataagc cagaggtact acataactat cagatccaag
    14881 attgcattct tgtatttttg atatatacag cgaaacatta tattcgtctt ctttccctgt
    14941 atctacttct aatcattgga tattatgcaa ttgttctgct attttataat tttcaacata
    15001 tttttttaat tctttacaaa ctgggcaatt ttttgaatga aaaattaaaa ttttatctga
    15061 taattctaca cttgttgtga cagtagttgt aggtgttgta ttctgtttag aaaaaattaa
    15121 tactaaataa acgccataac tacctaacaa aaataatata ataataagcc aaacaaaaaa
    15181 ttttttcatc tttttaagtt tattttattt aaaatttatt ttttactttt aatctccgac
    15241 tcccaactac tattatttaa caacaaaaat tacaaagttc tattatattt tttataatta
    15301 ctttatatat gttatttaat actgaaaaat tgggtgtaaa cacaattttt ctgtgccgag
    15361 ggcgagattt gaactcgcaa gaccttgcgg tcatcaccac ctcaaagtga cgtgtctacc
    15421 aatttcacca cctcggcaag cttatctgtg aataaagtag tatattttga tttacaaaat
    15481 ataccccatt ttcctcatta tattttatct aatataatta tgcttgttgt tcggattttt
    15541 ctgttgtttg ttctgttggt gtcgcgggtg tgatatcttg ttgtttatat tttgtttgat
    15601 attctttttt aagagccgtt ttggtttctc gcactgattt gttacggata tagtaaagct
    15661 ttgatttttt tactcttgga gattttatga tgtcaaaact agcaatgttt gaagaatata
    15721 ctggtatagt ccattctact ccaacaccat ctaaaattct gcgaatggta atatttgccc
    15781 ctaattcttt gccatgttta tgagaaataa caactccttg aattatttgg gttttatttt
    15841 tgtctttatt ttttgttttt tcttttaatt ttaggacaat tttaactgta tcgcccactt
    15901 tgacattagg aatgtttggt ttatacttat ttcgtaaaat ttcatccaat ttattagtat
    15961 ccatagttgt gttttagttt tattaatata ttattaatgg tgtttgtagt atttttatag
    16021 attatcatat tctaaacatt tttgcaactc tttgggtaag gttgacttaa aaaccatttt
    16081 ttctttttct caacaaaatt ctaatgattg ggcatgtaaa aattgtcttt ttattggaaa
    16141 aagtttatta attttctttg aaccatattc tttatcccca attataaaat gtgataatga
    16201 tgcaaaatgt actcttattt gatgtgtttt cccagtttct gggtatattt ctaatagcga
    16261 agtatttttg aattccttaa ttactttgta tcgagtaatt gcttggtggg atatattatt
    16321 attttttaaa tctattttgc gacggttgaa tttttgtttt gaacgagtaa ttgctaaatt
    16381 tattgtccct gttttttgtg gcattttacc ataaattaaa gcaatatatg ttttttgaat
    16441 taaatgtttt tgaaacattg tttttaattt ttgaaatgtc ttattgtttt tggctatgat
    16501 cattattcca gaagtatttt tatctaatct atgaacaatg cctgggcgca atatattatc
    16561 acctacatta cgaatagcag gatagtaagc taataatcca tttactagag tattagtatc
    16621 agatattcgc aacgggtgta ctgttagatt agcgggtttg tttataacta tgaaatcatt
    16681 attttcaaaa acaatatcta tttttatatt tggatttggt tttaattctg gttgttctaa
    16741 aattcaattt attaaaataa tatcaccagt ttttacttta tatgctggct tataaatttt
    16801 tttattattc aaaattacac cttgtgtttg aataaaatgt tcaattttat tgcgagttaa
    16861 tttgtataat tgtagatgta ttgttttatc taacctattt tgtttagttg ctataatttt
    16921 tattttcata atttaaattg taattagact aattagctcc gaattaatta gtatcaaaac
    16981 taaattatat tatttcccaa gtatatctta ttgtaataca aaacggatct aatatatttt
    17041 ttattctact ttttataaaa ttagtcaagt tatataggaa tgtttagtgg gcaatacagg
    17101 actcgaacct gtgacctcta caatgtcaat gtaacgctct aaccaactga gctaattgcc
    17161 ctcttggata atatatgttt atactatcaa atttattttt ttctttcaag tgcaattggt
    17221 aaatgaaatg gttttagaat taaattggta attcatacca ataccaatgc tactataatt
    17281 cctaataata tatcgcttgg tcaatgcata ccattatata ttcttgatca gcccataatc
    17341 aaaataaaaa acaaacaggg tcataaagtc gggttgctaa taacaagcgc gaacaatata
    17401 gagcttataa aagatacatg cccagaaggt accgcgcttt ttgtaataga tgtaccaata
    17461 gtatagacat ttggcaatac catataagga cgtggagtaa acacaatatt cttcgcaatt
    17521 atattgataa ttagcacatc tagcaatata gccaaaataa ttaatgaaat agttttgatt
    17581 ttataaataa ttttatgcga gaatataaca tataaaaata ttataccaac tatacatatt
    17641 ataaaaatat aactactaat gatagtagat ataatatcta aaatttgact tctgttatta
    17701 tttattaaac gaaataaaaa aatattcata aaattagatt gtatccgcca ccatatatca
    17761 acactttctt tattatgtat ttattttctt ggtggctggg ctagggattg gacctaggac
    17821 ctgttgttta tatggggcga atactatata ttttgatttt ataatggtat ccgccatcat
    17881 atatcaacaa tttctttatt atgtatttat tttcttggtg ggcgtgctag ggattggacc
    17941 taggacctgt tgtttataag acaaccgctc taccactgag ctacacgccc atcaagaaaa
    18001 taaattatta atgttaattg ttgatataag acaaccgctc ttccggggta aactatttat
    18061 ttctattgtg
//
LOCUS       DAOTJA010000004        17792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_3583_length_17792_cov_6.180019, whole genome shotgun sequence.
ACCESSION   DAOTJA010000004 DAOTJA010000000
VERSION     DAOTJA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17792
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_17792_cov_6.180019"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..518)
                     /locus_tag="P5241_00540"
     CDS             complement(21..518)
                     /locus_tag="P5241_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRV32069.1"
                     /translation="MFSYNGLVAKKQRVKEASSDILVQLKRRFDLIPNLVETVKGYTK
                     HEAETLEKITEMRSKINDSKNIIETDKINKEISKDIKNFLVSVEAYPELKANENFLQL
                     QKELADTENKIQASRRFYNNIVKEYNIAIHSIPTNLVASLLKYTEEPLLDISEEEKES
                     IQVRF"
     gene            complement(826..1062)
                     /locus_tag="P5241_00545"
     CDS             complement(826..1062)
                     /locus_tag="P5241_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32070.1"
                     /translation="MSKKTNKFITPKKNLNTYFLIVSILLVGICGFVLFHTVGFLIRN
                     ANDILSVNTGKLEKTKEFNMKVFEELKSKNPLNK"
     gene            complement(1069..1302)
                     /locus_tag="P5241_00550"
     CDS             complement(1069..1302)
                     /locus_tag="P5241_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32071.1"
                     /translation="MRIEQILIDYEDVQSRTSSESLGSGNVSASTTIKPMSLEEELMA
                     KRKSTTTTKKKTTTTTKITDRYKMNILGKVYIK"
     gene            complement(1330..1788)
                     /locus_tag="P5241_00555"
     CDS             complement(1330..1788)
                     /locus_tag="P5241_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32072.1"
                     /translation="MAQLISENTNKKSKKPLSVFKKKLIIYISINLIVILIFCIFYYQ
                     YSKIIISEVSAVTDKKKSQADVNLLADYISRLEKDYKQVLEEFGPYLDLLPAKDDLLD
                     FKDSVISIAKKYKLDPAFSFGVENSATEKEPKSYGFTLIISGTTANLLSF"
     gene            2093..3007
                     /gene="dprA"
                     /locus_tag="P5241_00560"
     CDS             2093..3007
                     /gene="dprA"
                     /locus_tag="P5241_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRV32073.1"
                     /translation="MNPEKEYAKLENHNIKLILKNDPDYPLQLKEISHAPLGIYVKGN
                     IDILQRTTNIFLLAVVGSRKTTDYGQEVCETVIRDLSIYNFIIVSGLAYGTDAHAHQN
                     ALNNNLKTIAVLGSGIENLYPKINQNLAQNIIEHDGAIISEYPINSSSQYFHFPQRNR
                     IISGLSQGVLIIEAKEKSGSLITAKLALEQNREVFAIPNSIFEKSSWGTNKLIQSGAK
                     LVLDANDILDELNILAPQTQSNIFNQQNLNEQEKLIFDFIQKEDSVDLDALAENLEDL
                     QINEILASLTSLELKGLVEHNNDEYSIL"
     gene            3032..3310
                     /locus_tag="P5241_00565"
     CDS             3032..3310
                     /locus_tag="P5241_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV32074.1"
                     /translation="MPKNLIIVESPAKAKTISYFLDKTSYEVVACMGHLRDLPKGTLG
                     IDIENNFEPKYIIPKTKTKIVTNLKKIAKNKDNIILATDEDREGEAIA"
     gene            3509..3607
                     /locus_tag="P5241_00570"
     CDS             3509..3607
                     /locus_tag="P5241_00570"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRV32075.1"
                     /translation="MKGLSAGRVQTPALRLIVEREQEIANFKPQEY"
     gene            3839..4645
                     /locus_tag="P5241_00575"
     CDS             3839..4645
                     /locus_tag="P5241_00575"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRV32076.1"
                     /translation="MLRFPAKKTMIVAQHLYENGFITYMRTDSLNLSTASIQNARDYI
                     YKTFGKDYVPEKPNYYKAKSKLAQEAHEAIRPTNPSRTIKNIEKSLNKDELKLYNLIW
                     QRFIACQMSNAKIEKQNILTQTINTKNIYTFKSEYQKICFDGFLKILPLDIKNSGILN
                     LKENDIYKINKVLPSQHFTEPPARYNDASLVKTLEKYGIGRPSTYATIISTLLERGYV
                     SYNENKSFVPSDIGIKTCNILVQHFPDIADYQMTANLERELDLIAEGKKN"
     gene            4751..5098
                     /locus_tag="P5241_00580"
     CDS             4751..5098
                     /locus_tag="P5241_00580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRV32077.1"
                     /translation="MCPQCGAPLIIKISRFGKFLACSKFPECKFTKSIDQETGIKCPK
                     CKTGNIIMRKTKKGKIFYGCSNYPKCDFIINKKPLQDTCPQCHYPLIQTNKTTYKCSN
                     KNCKYVTRTNTNI"
     gene            5070..5537
                     /locus_tag="P5241_00585"
     CDS             5070..5537
                     /locus_tag="P5241_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV32078.1"
                     /translation="MLPELTLIYNNTKDYLSSEEWGFIEKAYLLSKTYHQGQKRKTGE
                     DFVNHPLRVAIELSQLKQDASTIIAGLLHDILEDTNITTDEIKKEFGDEILFLVQGLT
                     KIHSLHHKVKTRQQYHQKKHYLDNLRSLFLAASQDYRIIIIKLMDRLDNMRTI"
     gene            5682..6110
                     /locus_tag="P5241_00590"
                     /pseudo
     CDS             5682..6110
                     /locus_tag="P5241_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6267..6476
                     /locus_tag="P5241_00595"
     CDS             6267..6476
                     /locus_tag="P5241_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRV32079.1"
                     /translation="MFVLTPKGDVINLPKGSTPIDFAYKIHSDLGNHCQMAKVNNQIV
                     PLNYQLQSGDIVEIITNKNKKPNRE"
     gene            complement(6693..6944)
                     /locus_tag="P5241_00600"
     CDS             complement(6693..6944)
                     /locus_tag="P5241_00600"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32080.1"
                     /translation="MPPKIELMYPGGDLTSDTSSVEIIGKVLRAKEFTINDQIINFDH
                     DGVFNYVLALEPGLNIITIKAKNNSGKSSQIIRNVIYNK"
     gene            complement(7023..7337)
                     /locus_tag="P5241_00605"
     CDS             complement(7023..7337)
                     /locus_tag="P5241_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV32081.1"
                     /translation="MKDNNIVHLGKILKESREQQNITLEKMALKTRIKMDHLINLEDE
                     HFEKLPPKIFIKGIISKYCDICGLDKKEMLKQFDNYLIESAGSNNKYNKLFTEHKKIY
                     PK"
     gene            complement(7446..9095)
                     /locus_tag="P5241_00610"
     CDS             complement(7446..9095)
                     /locus_tag="P5241_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRV32082.1"
                     /translation="MALLPKKNNYSNVEAGSLVVLPEETLSDTKQLEAIENNTSVKTE
                     KLEEVNIAKNEIHEKELNLLAKQKFANYKLPPIDLLENDSGKPTVGDVRANANIIKRT
                     LQNFGIEVEMGEICIGPTVTQYTLKPAEGVKLSRITALQNDLSLALAAHPLRIEAPIP
                     GKSLVGIEVPNKSVALVKLRNMLSTEEFQTSKALLLVPLGRDVSNKARYINIAKMPHL
                     LIAGATGTGKSVCIHTIITSLLFRNTPETLRFILIDPKRVELSHYEGIPHLLTPVITE
                     CKKALPALRWAISEMERRYGLLLANHSRDIESYNEKISKKDVDVLPYIIIIIDELADL
                     MMQYGKEVEGAIVRLAQMSRATGIHLVISTQRPSVEVITGLIKANITSRIAFQVASQV
                     DSRTILDSQGAEKLLGHGDMLYLAADTSKPVRLQGAFLTENEVNRVTNFIRKQDVNIE
                     SNFGLETTLKGQEILNNKAPNQDSINLDEYAYGEEDEKYEEALEVIKQFGKASASLLQ
                     RRLGIGYARAAKLLDILEEKGIIGPADGAKPREVFLSNSQV"
     gene            complement(9099..9599)
                     /locus_tag="P5241_00615"
     CDS             complement(9099..9599)
                     /locus_tag="P5241_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32083.1"
                     /translation="MSHSGKNGKKKQHVELDKKTNLLADLTAEVKIGIFAILYFCVAI
                     ILILSFFDLAGPVGEALYNFLNKLVGIGYFLLPLSLLILSIQMLKSRTRHYFKSAVVI
                     IGLVLMVLSLLGIIDIIHETQGGYLGRFSAMITIPFGVWAGVIILICVFIIGLLMAFE
                     KPLSFA"
     gene            complement(9925..10092)
                     /locus_tag="P5241_00620"
     CDS             complement(9925..10092)
                     /locus_tag="P5241_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32084.1"
                     /translation="MKNPLLGSALIALAGPVANFVLAFLFAMILRFNYIFNLNKNVFE
                     FISFIVLINCL"
     gene            complement(10123..10356)
                     /locus_tag="P5241_00625"
     CDS             complement(10123..10356)
                     /locus_tag="P5241_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32085.1"
                     /translation="MSGSNEIVIVLLRYVVILYSLILHEVSHGTMALILGDTTAKDLG
                     RLSLNPLRHLDPVGSFLVPIFSFIITGFKVAFG"
     gene            complement(10366..10908)
                     /locus_tag="P5241_00630"
     CDS             complement(10366..10908)
                     /locus_tag="P5241_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32086.1"
                     /translation="MVEPSISNIIKILADASANQQVVNVSTLVFNLRRIGLFVIIVLI
                     VGIIFLFVKLKNLSKLAQAVKQSIQKPKINDKIIKRFFESIYHKIQDNNSLMNKLVLA
                     ETENYFDEVLKEIGFQGKSLSDKLDNIQEQFLPNLEQIKIAHNQVQNIFDQENYQLGK
                     EETNNIVLIYKQGIENLIKL"
     gene            complement(10923..12356)
                     /gene="gatA"
                     /locus_tag="P5241_00635"
     CDS             complement(10923..12356)
                     /gene="gatA"
                     /locus_tag="P5241_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRV32087.1"
                     /translation="MIIENIQNDLKNKVYSCVDMVKQRFDVILKENANLNAFISMCRD
                     EAIKTAEHIDQDIKNDIPLKPLSGVLFGIKDNILVKDTITTAGSKILEDYIAPYDATV
                     VKKIKDAGGIIIGKTNLDEFAMGSSNETSAFGNCHNPYKLDKVPGGSSGGSAVAVSKE
                     LVHIGLGSDTAGSVRQPASFCGVVGFKPSYGAISRFGLIAMSSSLDVIGILSKYVSDC
                     EKVFQVVCGKDKMDSTSTDILSVLPNNIPNVSIGIPKEYFVSGINEEVEKSVRDFITS
                     LKNAGFRIKEISLPHTEYSLPTYHIIMPAEVSANLARFDNIRYGTDFHQLQMSLGIEN
                     LNDLYYKTRGAKFGREVKRRILIGTYVLSKGYYDAYYIKAQKVRTLISQDFENAFKEV
                     DFILTPTSPTTAFNIGEKNQDPVAMYLSDMFTVSANLAGLPAVSLPLAKDLEGLPIGL
                     QIIGKRNEDFKVFDLAKNLEKLINFSF"
     gene            complement(12386..12673)
                     /gene="gatC"
                     /locus_tag="P5241_00640"
     CDS             complement(12386..12673)
                     /gene="gatC"
                     /locus_tag="P5241_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRV32088.1"
                     /translation="MILKEDIGRLLELARLDISEEEKLQLTTDVSRILEYVDKLSTID
                     VSNIVGNTGGSLNKNIVRNDEFMPFDFDREVLINDIPCKQDNLVVVPKIRD"
     gene            complement(12738..13028)
                     /locus_tag="P5241_00645"
     CDS             complement(12738..13028)
                     /locus_tag="P5241_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32089.1"
                     /translation="MSKSIVDFFCDNNTKKFFDKLDRAGIHIQNEQILSSNSNFKDLK
                     FIFTGELKSMTRAEAQEKVKELGGIVKETMSRDIDYVIVGEHPGNKLEKAKQ"
     gene            complement(13056..14723)
                     /gene="ligA"
                     /locus_tag="P5241_00650"
                     /pseudo
     CDS             complement(13056..14723)
                     /gene="ligA"
                     /locus_tag="P5241_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     gene            complement(14751..15131)
                     /locus_tag="P5241_00655"
     CDS             complement(14751..15131)
                     /locus_tag="P5241_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32090.1"
                     /translation="MYYNTNKKDKKHRIGQIYLMSALLLAGASALGLALISVYLRDLR
                     LSYEVGESTRAIFAADSAIEYQLYMVLNNSDIIEIPIMSNNTSYTSSIENDVLRGVGE
                     SRGIRRGIEIYLGANNLDNMSSEE"
     gene            complement(15175..15705)
                     /locus_tag="P5241_00660"
     CDS             complement(15175..15705)
                     /locus_tag="P5241_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV32091.1"
                     /translation="MIHKSKKLKSFTLVEVLVSLFLFVVITGILTQIYIATVRSERIA
                     YVLLRDENIIRNELEVLARDIRMGKSFSSNGSGDSLDYLTYYDNSWHRISYVFDAEHN
                     TIRKSVSDDIEYVSNDYVIIIPENIKIDTLKFYVNKLYNEQPSITITLTIESQAYNKK
                     YTTSLQTTVTPRMLDL"
     gene            complement(15809..16291)
                     /locus_tag="P5241_00665"
     CDS             complement(15809..16291)
                     /locus_tag="P5241_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32092.1"
                     /translation="MISKVNQKSGFTLIESLLAIGVLIVGVLSAFILVVRSLSNIPLI
                     QSRLIAANLAQEGIELIRQKRDTNYIQGLDWINGFNDQCICMDNSQLADECYFVIDAN
                     SRTLKQYDNDDYLKFNKELGIYSYDTGEILPYFFQRKIEICFPLQNNNKEMRIKETVY
                     "
     gene            complement(16507..16659)
                     /locus_tag="P5241_00670"
     CDS             complement(16507..16659)
                     /locus_tag="P5241_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRV32093.1"
                     /translation="MGAAAKEYSISYNQLIKLLKEHHIELDRKVLSELAQNYNDIFKE
                     IVTKIK"
     gene            complement(16854..17030)
                     /locus_tag="P5241_00675"
     CDS             complement(16854..17030)
                     /locus_tag="P5241_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32094.1"
                     /translation="MNKKFFNKRVRITKNNKVIRRVSNTAHTTSSDSSKTKNRRKKAR
                     SFHKTIIKSLNKIV"
     gene            complement(17055..17543)
                     /gene="infC"
                     /locus_tag="P5241_00680"
     CDS             complement(17055..17543)
                     /gene="infC"
                     /locus_tag="P5241_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRV32095.1"
                     /translation="MAPQEVHLIGLDGANLGIVSYEEAKRIAQEQNCDLVKISDKVLP
                     YIYKLGDYGKIKYLEEKKRRKEQLKHKQDIEKIIRINFNESLHDLETKAKKATEFLEE
                     GRRVRIEMRLTGREKMHTDISRNKFITFLGLIKVPFKYLKNISIMSGFCDATIIKDNL
                     VK"
BASE COUNT         6487 a         2993 c         2048 g         6264 t
ORIGIN      
        1 agaaattaaa aattattata ttagaatcta acttgaatac tttctttttc ttcttcgcta
       61 atatctaata atggttcttc tgtatatttc aataaagaag ccacaagatt ggttggaata
      121 gaatgaatag caatgttata ttcttttact atattattgt aaaatcgacg actggcctga
      181 attttatttt cagtatcagc taattctttt tgtaattgta agaaattttc attagctttt
      241 aattctggat acgcctcaac gcttactaaa aagttcttaa tatctttgct aatttctttg
      301 tttattttat ctgtctctat aatattttta gaatcattaa tttttgaacg catttcagta
      361 attttttcta atgtttctgc ttcatgctta gtatatcctt ttactgtttc tactaaatta
      421 gggatcagat caaatcgtcg ttttaattgc actaaaatat ccgaagaagc ttctttaact
      481 ctctgctttt tagctaccaa accattatat gaaaacacaa atcagcatat taaaataact
      541 aaaatcaata taattaacat attaatagtt taaaaattat ttatctaata atttaataat
      601 aacaaacgaa ataagagatg ccacaaatag caaccctaaa gtagcaaacg acctttctat
      661 tatctgtgga gtaaaaactc ctcaaatagc caataaacct accaaagtaa atatcataac
      721 taatataatc atagtatatg aaatcacttt tttcagtaaa gatgtattca tattttgtat
      781 ttagataaat tattcaaata ttattaaacg aaacaaccga ccttgttatt tattaagtgg
      841 attttttgat tttaattctt caaaaacttt catattaaac tcttttgttt tttcgagctt
      901 gccagtatta acactcaaaa tatcattggc atttctaatt aaaaacccca ctgtgtgaaa
      961 caaaacaaaa ccacatattc caaccaacaa aatagacact atcaaaaaat aagtatttaa
     1021 gttctttttg ggagtaataa atttatttgt ttttttagac atattatttt atttaatata
     1081 tacttttcct aaaatattca ttttatatct atctgttatt ttagtagtgg tagtagtttt
     1141 ctttttagtg gtagtggttg attttctttt ggccattagt tcttcttcca aactcatagg
     1201 tttaatagta gtactagcag acacattacc agaacccaaa gattctgagg aggttcgaga
     1261 ttgcacatct tcataatcaa ttaaaatttg ctctatacgc acaatgtatt ttaatttagc
     1321 aaaatcagat cagaaactta ataaattagc agtggtacca gaaataatta aagtaaaacc
     1381 ataacttttt ggttcttttt ctgtcgctga attttctaca ccaaaactaa aagctggatc
     1441 caatttatat ttcttggcta tacttataac actatcttta aaatcaagta aatcatcttt
     1501 cgctggcaat aaatctaagt atggaccaaa ttcttctaat acttgtttat aatctttctc
     1561 taatcgagat atgtaatcag ccagtaaatt aacatctgct tgactttttt tcttgtctgt
     1621 tacagctgat acttcagaga taattatttt tgaatactga taataaaata tacaaaatat
     1681 taatatcaca ataagattta tagaaatata aataattaat tttttcttaa atacagaaag
     1741 tggtttttta gatttcttgt ttgtattttc agatataagt tgtgccatat tgaatttttt
     1801 ataaaaaaag tgtataatta gaataatatt attataacca cggcctataa atgtttatta
     1861 tactcaacac ttgactacaa actaaaataa ctatggtcaa tataagctat tctaagtact
     1921 taaattctat aatataatta tatcaaattt tgtaaacctt tgcaatatgc aagaacatat
     1981 ttcctacaat gcccttaata tcctatttaa tggcgctttt gcacacttag aacaagaata
     2041 taacaaatat ggtgattatg aaactacttg aaaaaaacaa aacaattttg ttatcaaccc
     2101 agaaaaagaa tatgcaaaat tagaaaacca taatatcaaa ttaattctta aaaatgatcc
     2161 cgattacccc ttgcagttaa aagaaattag ccatgctcct ttgggtattt atgtcaaagg
     2221 aaatatagat attctacaaa gaacaaccaa catctttctc ttggcagtag ttggctcaag
     2281 aaaaacaaca gattatggtc aagaagtgtg tgaaacagtt atacgagatt tatcaattta
     2341 caattttatt attgtaagtg gattagctta tggtactgac gcacacgcac atcaaaatgc
     2401 cttaaacaat aatctcaaaa ctattgctgt attgggttcg gggatagaaa atctttatcc
     2461 gaaaataaac caaaatttag ctcaaaatat tatcgaacat gatggtgcta ttatctcaga
     2521 atatcctata aatagctcgt ctcaatattt tcattttcca caacgcaata gaattattag
     2581 tggactaagc caaggagtat taataataga agccaaagaa aaaagtggat ctttaattac
     2641 agctaaattg gcactcgaac aaaacagaga agtttttgct attcctaatt ctatttttga
     2701 aaaatcaagt tggggcacca ataaattaat tcaatcaggc gcaaaacttg ttttggatgc
     2761 caatgatatt ttagatgaat taaatattct tgctccacaa actcaaagta atatttttaa
     2821 tcaacaaaat cttaatgaac aagaaaaact tatttttgat tttattcaaa aagaagattc
     2881 tgtagattta gatgctctgg cagaaaatct tgaagacttg caaataaatg aaattttagc
     2941 tagtcttaca tcattggaac ttaaaggtct tgtagaacac aataatgacg aatattctat
     3001 attataacac ataacacatt aaacaaacaa tatgccaaaa aacttaataa tagttgaaag
     3061 cccagccaaa gccaaaacta tatcttattt tttagataaa acatcttatg aggtagttgc
     3121 ttgtatggga catttgcgtg atttaccaaa aggtactttg ggaattgata tagaaaataa
     3181 ttttgaacca aaatatatta ttcccaaaac taaaacaaaa atcgttacta acttaaaaaa
     3241 aatagccaaa aataaagata atattattct ggcaaccgat gaagatcgcg aaggtgaagc
     3301 catagcttga caccttactc atatactaaa tcttaatgtt aacaacacca aaagaattgt
     3361 tttccacgaa attacagaaa ccgccataaa agaagcatta aaaaatccga gaactataaa
     3421 tcttaattta gtagattccc aacaagctcg ccgggttctt gatagattgg taggttataa
     3481 attatcccct tttttgtgaa aaaaaattat gaaaggcctt tctgctggca gagttcaaac
     3541 acctgctttg agattaattg ttgaacgcga acaagaaata gcgaatttta aaccccagga
     3601 atactgagaa attcaagcag aatttcaaaa aaataatcaa atatttttag gtaatctaac
     3661 acaaattaac aacaaaaata tagaaaaatt tgatcttaaa aatcaacaag aatgtcaaca
     3721 aattaccaat gaaatccaag accaaaatgt aaaaattaca gagatttcta ctaatataat
     3781 caacaaaaaa cctttacccc cttttactac tagtacttta caacaaacca gttgacaaat
     3841 attacgtttc ccagccaaaa aaacaatgat agttgctcaa cacctttatg aaaatggttt
     3901 cattacttat atgcgtactg actctttgaa tttgagtact gcttcaatcc aaaatgccag
     3961 agattatatt tataaaacat ttggcaaaga ttatgtacca gaaaaaccaa attattataa
     4021 ggcaaaatca aaattagccc aagaagcaca tgaagctatc agacctacaa atccctcacg
     4081 cacaataaaa aatatagaga aatctttaaa taaagatgaa ttaaaattat ataatttaat
     4141 atggcaacga tttattgctt gtcaaatgag caatgccaaa atagaaaaac aaaatattct
     4201 gacccaaact ataaatacaa aaaatattta tacttttaaa agcgaatacc aaaaaatatg
     4261 ttttgatgga tttttaaaaa ttttaccttt ggatataaaa aattctggta tcttaaattt
     4321 aaaagaaaat gatatctata aaataaacaa ggtattacct tctcaacatt ttaccgaacc
     4381 tcctgccaga tataatgacg catctttggt caaaacattg gaaaaatatg gcattggtag
     4441 accatctact tatgctacta ttatttctac tttgcttgaa agaggatatg tatcttataa
     4501 tgaaaataaa tcatttgttc cttctgatat tggcatcaaa acttgtaata tacttgtgca
     4561 acattttcct gatattgctg attatcaaat gacggctaat ttagaaagag agttggactt
     4621 aattgctgag ggtaaaaaaa attgacgcca actgattaaa aatttttatt tcccttttga
     4681 aaaaaatctt gaaacaaaat accaaaaagt acaaaaaata aatttaattg aagataacaa
     4741 aaccaatgaa atttgcccgc aatgtggagc accattaatt ataaaaataa gcagatttgg
     4801 aaaattttta gcttgttcaa agttccctga atgtaaattt actaaatcta ttgatcagga
     4861 aactggtatt aaatgtccaa aatgtaaaac tggcaatatt ataatgcgta aaactaaaaa
     4921 aggaaaaata ttttatggat gttccaatta tccaaaatgc gattttataa ttaacaaaaa
     4981 gccattacaa gatacatgtc cgcaatgtca ttatccactt atacaaacaa acaaaactac
     5041 ttataaatgt tcaaataaaa attgtaaata tgttacccga actaacacta atatataata
     5101 ataccaaaga ctatttatca tcagaagaat ggggctttat agaaaaagct tatcttttat
     5161 ctaaaacata tcaccaagga caaaaaagaa aaacaggaga agattttgta aatcatcccc
     5221 tacgagtagc catagagcta tctcaattaa aacaagatgc cagcacaatt attgcaggat
     5281 tattacatga tattttagaa gataccaata tcacaaccga tgagataaaa aaagaattcg
     5341 gcgatgagat tcttttttta gtgcaaggac taaccaaaat tcatagccta caccataaag
     5401 taaaaacacg ccagcaatac catcagaaaa aacactatct cgacaattta agaagccttt
     5461 ttcttgcggc gagtcaagat tatagaataa taataatcaa gcttatggat agactggata
     5521 atatgcgaac tatttgagcc ttaaacaaag aatctcaaca aaaaaattca ttagaaacat
     5581 tagatattta tagcccatta gcttattatt taggtttaga aaatatcgca tcaagtttag
     5641 aagaattggc atttccttat gcatatccca aagaatacaa aatggtaaaa aaatatgttg
     5701 gtaaaaaata tttagatctt gaaaaggctc taataatttc taaacgtgaa ctaagtgatt
     5761 atcttaacaa aaataattgt aatttttttg atatagaatt tcgtaaaaag aaactgtata
     5821 gtatttatcg aaaatatcta aaagaaaaag attttaataa aatttatgat attatagctc
     5881 tacgaattat tactcaaaac atcgaagatt gttatttaat tttgggtttt attcatcagt
     5941 tatgactacc tctgcccggg cgtttaaaag attatattgc tcttcctaaa ccaaatggct
     6001 acagagcaat tcatactact acacttacta caaacaatca aattgtcgaa atacaaatcc
     6061 aaacaatgga aatgtatgag tattctaaat acggtatcgc gagccactga ttatatcaat
     6121 taaataaaga aaacaaaagt tatatcaaaa gaaaacaaat tctaaaagaa aaccccttac
     6181 ttaaaactct ccaaactatc aatctcaaaa accaagatgg acaaactatc tacaatattt
     6241 ttgttaatga gctactaaaa gaacaaatat ttgtactcac accaaaagga gatgttataa
     6301 atttaccaaa aggatcaaca cctatagatt ttgcatataa aattcatagt gatcttggaa
     6361 atcattgcca aatggccaaa gtaaataatc aaatagtacc actaaactat cagttacaat
     6421 caggagatat tgtggaaata attacgaaca aaaacaaaaa accaaataga gagtgaatga
     6481 aattcacaaa aataaaatca acaaagagaa aaatattgga taatctagaa taaagaatac
     6541 agaaataaat aataaataag gaaaggacaa caaaaaaatc aaataaatcc agaataagaa
     6601 gaaaaacaga ggcaatgggg aaaaaaccaa ataaatcaaa aatacaggaa ccaaactcct
     6661 gtatttttta atccaacgca attcaaaatt acttatttat tatatattac gtttctaata
     6721 atttggcttg atttcccact attatttttg gctttaatag ttataatgtt caaaccaggc
     6781 tcaagtgcta acacataatt aaaaactcca tcgtgatcaa aatttattat ctgatcattg
     6841 atagtaaatt ctttggctct taacacctta cctataattt caactgaaga agtatcagaa
     6901 gttaaatctc ctccaggata cattaattca atttttggag gcaataataa attaaatact
     6961 tgtcataaaa taaaacacgc cactacaata gcaaaaaaaa ttaaaaatat ttttgataaa
     7021 tttcattttg gataaatttt tttatgttca gtaaataatt tattatactt attatttgat
     7081 cccgcacttt ctattaaata gttatcaaat tgtttaagca tttctttttt atccaatcca
     7141 caaatatcac aatattttga tattatacct tttataaaaa tctttggggg taatttttca
     7201 aaatgttcat cctccaaatt tattaggtga tccattttga tacgagtttt caaagccatt
     7261 ttttcaagag taatattttg ttgctcacgg ctttctttta atatttttcc taaatgcaca
     7321 atattattat ctttcataaa attaaattaa attttttcat tattatcatc tggttcatta
     7381 ttatcatcta ataaattctc tgtatcagac gagtccaata aactttcctt ggtactagta
     7441 ttttttcata cttggctatt tgataaaaat acctctcttg gtttagcacc atcagctgga
     7501 cctataattc ctttttcttc taatatatct aataatttag cagctctggc atatcctata
     7561 ccaagccttc tttgcaacaa tgaagccgag gctttaccaa attgttttat aacctccaaa
     7621 gcttcttcat atttttcatc ttcttcacca tatgcatact catctaaatt tatactatcc
     7681 tgattgggcg ctttgttatt taaaatctcc tgccctttta atgttgtctc caatccgaaa
     7741 ttactttcta tattaacatc ttgtttacga ataaaattgg ttactctatt aacttcattt
     7801 tcagtcaaaa atgcaccttg caacctaaca ggctttgaag tgtcagctgc taaataaagc
     7861 atatcaccgt gtcctaataa tttttctgct ccctgactat ctaaaatagt tctcgaatct
     7921 acctgagacg caacttgaaa agcaattcgg ctagtaatat tagcctttat taatcctgta
     7981 atcacttcta ctgagggtct ttgagtagaa attactaaat gaatacctgt ggcgcgtgac
     8041 atttgtgcta atctaacaat cgcaccctca acttctttac catattgcat cattaaatct
     8101 gccaattcat caatgataat aataatataa ggtaaaacat ccacatcttt ttttgatatt
     8161 ttttcattat aactttctat gtctctacta tgattggcta acaacaaccc ataacgcctt
     8221 tccatttcac ttattgccca acgcaaagca ggcaaagcct ttttacattc agtaattaca
     8281 ggagttagca aatgcgggat accttcgtaa tgcgatagct ctactctttt tggatcaatc
     8341 aaaataaatc ttaatgtctc aggagtattt ctgaataata aagaagtaat tattgtatgg
     8401 atacaaactg atttgcctgt tccagtagca ccagcgataa gtaaatgagg catttttgca
     8461 atattaatat accttgcttt attgcttaca tctcgcccca atggaactaa caataaagct
     8521 ttagaagtct gaaattcttc tgtgcttaac atattgcgca attttactaa tgctactgat
     8581 ttatttggta cttctatacc aactaatgat ttaccaggaa taggtgcttc tatacgtaaa
     8641 ggatgagcag ccaatgccaa tgataaatca ttttgtaaag ctgtaatacg tgataatttt
     8701 actccttctg ctggttttaa agtatattga gtaacagtag gcccaataca aatttccccc
     8761 atttctactt caataccaaa attttgcaaa gttcttttta taatattagc attcgctctt
     8821 acatctccaa cagttggttt acctgaatca ttttccaata aatctatagg gggtaattta
     8881 taatttgcaa atttttgttt tgccaataag tttaactctt tttcatgtat ttcatttttt
     8941 gctatattaa cttcttctaa tttttcagtt tttacagatg tattgttctc tattgcttct
     9001 aattgctttg tatcactcaa tgtctcttct ggtaacacta ccaaagaccc tgcctcaaca
     9061 tttgaataat tatttttctt tggcaacaag gccaagaatc aagcaaaact taaaggcttt
     9121 tcaaaagcca ttaaaaggcc aataataaat acacaaatca aaataatcac tccagcccaa
     9181 acaccaaaag gtatagtaat catcgcacta aatctcccta aatagcctcc ttgagtttcg
     9241 tgaattatat caataatgcc tagcaaactc aaaaccatta acaccaaacc aataattact
     9301 acagctgatt taaaataatg tcttgtccgg ctttttagca tttgaattga taaaattagc
     9361 aaacttaatg gcaataaaaa gtaacctata ccaactaatt tatttaaaaa attatacaaa
     9421 gcttcaccaa ctggaccagc taagtcaaaa aaacttaaaa ttaaaattat tgctacacaa
     9481 aaatacaaaa tagcaaaaat gccaattttt acttcggcag ttaaatcagc taaaagattt
     9541 gtttttttat ctaattcaac atgctgtttt ttctttccat tttttcctga atgagacata
     9601 attaaaacat ttaatcaaaa aatatatttt acactatagt tatattatag cacagaaaca
     9661 ataaaaagta aacaataaaa tcttatcgta attatattat tgatatatat tatactattc
     9721 taactacata ggtggcattc ctgtaaatac aataaacacc aaagaggcaa tataaattat
     9781 aatattgtat ccaaacaaaa taataagcaa aaacaatgca aaactaaatt tgttcaaaaa
     9841 ttgttctaac ttattacctt tggtaaaata aaacaaaatt tttgagccat caaatggtgg
     9901 tattggtatt aaattaaata ctcctcataa acaatttatc aatacaataa aagaaataaa
     9961 ttcaaaaaca tttttattaa gattaaaaat ataattaaaa cgtaaaatca tagcaaacaa
    10021 aaaagctaaa acaaaattcg ctactggtcc agctaaggct attaaagctg aacccaataa
    10081 aggatttttc aaattaaaag gatcgtatgg tactggtttt gctcagccaa aagctacttt
    10141 aaaaccagta ataataaacg aaaatatagg cactaaaaaa gaacctacag ggtctaaatg
    10201 ccttaaagga tttaaagaca acctgcccaa atctttggct gtagtatctc ctaatatcaa
    10261 agccattgta ccatgtgata cttcgtgtaa aatcaatgaa tacaaaataa caacatagcg
    10321 taataacaca ataacaatct catttgaacc tgacataagt ttagtttaaa gtttgattaa
    10381 attttctatc ccctgtttat aaattaacac aatattatta gtttcttctt ttcctaactg
    10441 ataattttct tgatcaaaaa tattttgtac ttgattatgg gcaattttaa tttgttcgag
    10501 attaggcaaa aattgttctt gaatattatc taatttatct gataaacttt ttccttgaaa
    10561 acctatttct tttaatactt catcaaaata attttcggtt tctgccaata ctaatttatt
    10621 catcaaagaa ttattatctt gtattttgtg atatattgat tcaaaaaaac gtttaataat
    10681 tttgtcattt atttttggtt tttgaataga ttgtttcacg gcttgtgcta attttgataa
    10741 attttttaat tttacaaaca aaaatataat accaacaatt aaaacaataa tcacaaacaa
    10801 gccgattcta cgcaaattaa ataccaatgt agaaacatta acaacctgtt gattagcaga
    10861 tgcatcagcc aaaatcttta taatatttga tatgcttggt tctaccataa tataaattaa
    10921 tattaaaatg aaaagtttat aagtttttct aaatttttcg ctaaatcaaa caccttaaaa
    10981 tcctcatttc ttttgccaat aatctgtaaa cctattggta atccttccaa atcttttgcc
    11041 aaaggcaaac taactgctgg taagccagcc aaattagctg atactgtaaa catatcagac
    11101 aaatacatag ctactggatc ttgatttttt tctccaatat taaatgctgt tgtgggactc
    11161 gttggtgtta aaataaaatc tacttcttta aaagcatttt caaaatcttg cgaaatcaat
    11221 gttcttactt tttgtgcttt tatataataa gcatcataat atccttttga taaaacataa
    11281 gtacctatca aaattcgtct ttttacttct ctaccaaatt ttgcaccacg agttttataa
    11341 tataaatcat ttaaattttc aatacccaaa ctcatttgta attgatgaaa atcagtacca
    11401 taacgaatat tatcaaaccg agcaagattg gcgcttactt ctgctggcat aataatatgg
    11461 tatgttggta aagaatattc agtatgtggc aaactaattt ctttgatacg aaatccagca
    11521 ttctttaaag acgtaataaa atctcttact gatttttcaa cttcttcatt tattccggaa
    11581 acaaaatatt ctttaggaat acctatagaa acatttggaa tattatttgg caaaacagat
    11641 aatatatcag tacttgtgct atccatttta tctttaccac aaactacttg gaatactttt
    11701 tcacaatcag atacatattt gcttaaaata cctataacat ccaaagatga actcatggct
    11761 ataagaccaa atcgagaaat tgcaccataa ctcggtttaa aacctactac accacaaaaa
    11821 cttgccggtt gtcgaacaga ccctgctgta tcagacccca acccaatatg tactaactct
    11881 tttgatactg ccacagccga accaccagaa gaacccccag gtactttatc taatttatat
    11941 ggattatgac aattgccaaa cgctgaagtt tcattagaag aacccatagc aaattcatct
    12001 aaattagtct taccaattat tatacctcca gcgtctttaa tttttttaac tactgtggca
    12061 tcatagggcg caatataatc ttcaagaatt ttagaacccg ctgtagtaat agtatctttg
    12121 actaaaatat tatcttttat accaaaaaga acaccagata atggttttaa aggaatatca
    12181 tttttaatat cctgatcaat atgttctgca gtttttatag cttcatcgcg acacatacta
    12241 ataaaagcat ttagattggc attctctttt aatataacat caaagcgttg cttaaccata
    12301 tcaacgcagg aatatacttt atttttcaaa tcattttgta tattttctat aatcatatat
    12361 ttagcttata aatctatata ttattttaat cccgtatttt aggtactact actaaattgt
    12421 cttgcttaca aggtatatca tttatcaaaa cttctctatc aaaatcaaaa ggcataaatt
    12481 catcatttct aacaatattt ttatttaaac ttcctcctgt attgccaact atattactga
    12541 catcaatcgt cgataattta tcaacatatt ctaaaatacg agatacatca gttgtgagct
    12601 gtaatttttc ttcttcacta atatctaatc tagccaattc taataaacgc cctatatctt
    12661 cttttaaaat catattgtaa aatttatttc ttttttatta aattaataaa ttcgttctcg
    12721 ttcaaaattt taatacctca ttgtttagct ttttctaact tattaccagg atgttctcct
    12781 actattacat aatcaatatc acgagacatt gtttctttga caataccacc caattctttt
    12841 actttctctt gagcctctgc acgtgtcata cttttaagct caccagtaaa aataaattta
    12901 agatctttaa aatttgaatt gctagacaat atttgttcat tctgtatatg tatacccgct
    12961 ctatctaatt tatcaaagaa tttttttgta ttattatcac aaaaaaaatc tacaatactt
    13021 ttactcacct tggggccaaa atcatatatt ttatttcact catcaatact aatcttactg
    13081 gctatagcca atacatcgcc tggtgtattt atactaccat atttttttaa tttttgcgag
    13141 ataccccttg ctgcttcaat acccaccaaa ggaatgccaa gcgctacaat aaatttttct
    13201 aaagttatat ttttagaatt atttatagct tgaataatat ttttactaga tttttcacct
    13261 aatctttcca aaacttttaa atctccttct tctaaactaa ataaatcagc actatccaca
    13321 attaaatgtt catcaactaa tttttgtaat aattttgaac ccacaccctt aatatcaaat
    13381 ccctgtttag acacaaaatg cttcaagttt tcttggcttc tagcaggaca ttttggatta
    13441 gggcatttaa ccaaaatacc tgagtcatct tggatagtta atgtatgaca tacaggacaa
    13501 taagaaggca ttttgaaatc tcgttcctcc cctgttctta atttatctaa tacttttgta
    13561 attttaggaa taacatcacc cgctctactt aacaccacat gatcataaat ttttaaaccc
    13621 agtcttttaa tttcctcctt attatgtaaa gtagctcgtg aaacttttgt tccaccaata
    13681 aaaactggtt tcaaaaacgc tactggagtt aaaataccag ttctacctac ttgtacctta
    13741 atatcttcta ctattgtgat tacttcttta gcaggaaatt ttaaagctat acccccacgc
    13801 ggtcccttgc caatattacc taacaaatta aaaattttat tatcattaat caaaattact
    13861 atcccatcta tttcaaacga taatttatct ctattttgtt caactttatc ataatattgg
    13921 aatacttcat ctaaattttt agcataataa ttttctttgt ttgtagtttt aaaacctaac
    13981 ttttcaataa tttcatgttc atctttatga gttttttgcc ctaaatccgt taccaattta
    14041 taagcaacaa aatctaaatg cctcgaagcg gtaatttttg aatttaattg acgtactgaa
    14101 ccagcggcta aatttctcgg gttggcatat aaaggtaatc ccaatttctc ttgctctttg
    14161 tttactcttt caaattcttt ttttgttaaa ataacttctc ccctgacctc agcatctaca
    14221 tctttaaata attttaatgg tattgtttta attgttttta aattttctgt aacatcttcg
    14281 ccaatataac catcacctct ggtagcaccc aaaacaaatt cacccttttt ataaattgta
    14341 ctaattgata atccatcaaa tttatattca caataaaaat ctatcgaggt atcagttggt
    14401 aataatttaa taatacgatt gtatcattct tgtacttcag ttttattttt ggcatcattc
    14461 aaagacatca tcttgtcttt gtggacaact ttattaaatt tatccaaaac tttaccccca
    14521 atccttactg taggagaatt gtttgttatg aattcaggat attgctcctc taaatcacgc
    14581 aactcgtgaa acattgcatc tcattctgcg tcagaaattt caggtttatc taatacataa
    14641 tataaatagt tatgacgttc tatctcttgt cttaattttt ttattcgttt ttttgcctct
    14701 tctttattta atttttggct cataataaat aaaatccaaa ttaaatatat ttattcttca
    14761 ctactcatat tatcaaggtt attagcgcct aaataaattt caataccacg cctaataccc
    14821 cgcgattcac caacacctct tagcacatca ttttcaatac ttgatgtata acttgtatta
    14881 ttgctcataa taggtatttc tattatgtct gaattattta ataccatata aagctgatat
    14941 tctattgctg aatcagcagc aaaaatagct cttgtagatt caccaacttc ataagataat
    15001 cttaaatcac gtaaataaac actaatcaac gccaatccca aagccgatgc cccagctaaa
    15061 agcaaagcgc tcattaaata aatttgccca attctatgtt ttttatcttt tttgtttgta
    15121 ttataataca taatataata attaaaatat gttgctaata aatataaatt atttttacaa
    15181 atccaacatt cggggagtaa cagttgtctg taaactcgta gtgtattttt tattataagc
    15241 ttgagattca atagttaagg ttattgttat gctcggttgt tcattgtata gcttattaac
    15301 ataaaacttt aatgtatcta tttttatatt ttcaggaatt attataacat aatcattaga
    15361 gacatactct atatcatcac ttacggattt tcttattgta ttatgttcgg catcaaaaac
    15421 atacgatatt ctatgccaag aattgtcata ataagttaaa taatccaaac tatcgcctga
    15481 tccattggag ctaaaagatt tacccattct aatatcacga gccaaaactt ctaactcatt
    15541 acgaataata ttttcatctc ttagtaaaac ataagcaatt cgttcagagc gaactgtagc
    15601 aatataaatt tgggtcaaaa taccagtaat aacaacaaat aaaaacaacg aaaccaaaac
    15661 ttctaccaat gtaaatgatt ttaatttttt agatttgtgg atcataataa caaattaaaa
    15721 aaataatcta atgactagaa gaaagcaatc cataataatc aaataaatga tcttcaacaa
    15781 ttagagaata attattttgt tttacttttc agtatactgt ttctttgatt ctcatttctt
    15841 tattattatt ttgtaaaggg aaacaaattt ctattttacg ttgaaaaaaa taaggtaata
    15901 tctcccctgt atcataacta tatataccta gttctttatt aaactttaaa taatcatcat
    15961 tgtcatattg ttttagtgtt ctactattag catctataac aaaatagcat tcgtcggcaa
    16021 gctgactatt atccatacaa atacactgat cattaaatcc atttatccaa tccaatcctt
    16081 gaatataatt agtatctctt ttttgcctta ttaactcaat accttcctga gctaaattgg
    16141 cagcaataag tctggactga atcaaaggaa tattactaag agaacgaact accaaaataa
    16201 aagcactcaa aactcccaca atcaaaacac ctattgccaa aagcgattca attagtgtaa
    16261 accccgattt ttgatttact ttgctaatca ttgtttaata tatttaacaa aatttctaat
    16321 tttataataa ttaatagtat gtttttaata ataataatta tctcaacact atattacact
    16381 atattaatta tagctaattt ttataaaaaa aacaaccccg ccctattttt gcctataaaa
    16441 ttcacactaa aacataaagt tttacaataa atttttacta acaaaaaaga aggcaaggct
    16501 gtataattat ttaattttgg tgacaatttc tttaaaaatg tcattgtaat tttgggctaa
    16561 ctccgataat acttttctat ccaattctat atgatgttct tttaataatt ttatcagttg
    16621 attgtaactt attgagtatt ctttggccgc agccccaatt tgaacagttc aaagtttacg
    16681 aaaatctctt tttttctctt ttctccccct atatgaatgt acgctagcat gcaatactgc
    16741 ttcatgcgct tgtctaaatt tagattttct gcctcactta aaacctttgg tatgtttcaa
    16801 aacattcttt tttcttttgt gggcgataac accagattta actcgtacca taattaaact
    16861 attttattta atgattttat aattgtttta tgaaaacttc ttgccttttt tcgacgattt
    16921 tttgttttag aactatcaga cgaagtagta tgggcagtat tgctaaccct tctaataacc
    16981 ttattgtttt tagtaatcct tactctctta ttaaaaaact ttttattcat attatactat
    17041 ttatctttta attattactt taccaaatta tcttttataa ttgtcgcgtc acaaaatcca
    17101 gacattattg atatattttt aagatatttg aatggtactt tgatcaatcc caaaaaagtt
    17161 ataaatttat tccgagaaat atcagtgtgc attttttctc taccagtaag acgcatttca
    17221 attcttactc gcctgccttc ttcaagaaac tctgtggctt ttttagcttt tgtttctaaa
    17281 tcatgaagac tctcattaaa gtttatacga attatttttt ctatatcttg tttatgtttt
    17341 aattgttctt tgcgcctttt cttttcttct aaatatttta ttttaccata atcacctaat
    17401 ttatatatat atggaagcac tttatcagaa atttttacta aatcacaatt ttgctcttga
    17461 gctatacgct tggcttcttc ataactaaca attcctaagt tagcaccatc taatccaatt
    17521 aaatggactt cttggggcgc cacaaatttt ggttttcaat tttgattata acccatttat
    17581 taatttaatt tttaattaaa ttcgatccga atttatgaat ttacaacaat aacctaataa
    17641 agaaaaatta tgcttattat tcttttaaat attttttctt ttttctaact attaaataca
    17701 aattactaat tagtaaaaaa ttggttaaaa gcataatttt ctgtggagat ggcgggaatt
    17761 gaacccgcgt ctaaaaaact ttctttaata ac
//
LOCUS       DAOTJA010000005        16578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_3889_length_16578_cov_6.002179, whole genome shotgun sequence.
ACCESSION   DAOTJA010000005 DAOTJA010000000
VERSION     DAOTJA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3889_length_16578_cov_6.002179"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..528)
                     /locus_tag="P5241_00685"
     CDS             complement(169..528)
                     /locus_tag="P5241_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32096.1"
                     /translation="MSLDVYDVDYGSNVSTIPTNTTSYSKELQMKFLNVQNGQIWNND
                     TQLQVQVTGGTDLIKQSVYLNNQYIGEMNLISQNQDIKTYGIMIYYNTLQPQNEIRVH
                     LQDSALDIIEDAITIYK"
     gene            complement(595..834)
                     /locus_tag="P5241_00690"
     CDS             complement(595..834)
                     /locus_tag="P5241_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32097.1"
                     /translation="MVAAPAWHEFMQYAITKNNNIEYFTKPQITAISKPMLNGQYINS
                     IGGSLQIHSILFYIDKNNPLGPIPRNPANDSQFYN"
     gene            complement(925..1926)
                     /locus_tag="P5241_00695"
     CDS             complement(925..1926)
                     /locus_tag="P5241_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV32098.1"
                     /translation="MSNLGYITKQEADKAKKEKVVFVAKNIRMIAPHFVNFIEQELVA
                     KYGEEAIQSGGLTVISTLDLDLQAKAEEVVKKYADLNERDYKVKNMALLSEDPKTGQI
                     LAMVGSRDFFNLENEGNFNAVFGLRQPGSSFKPFAYLTLFKKGYTDNTILFDLPTNFS
                     TDPQNPYTPKNYDKKFRGPINLRNSLAQSLNIPSVKVLYLAGITDTINTAKSFGITTL
                     TQDANYYGLPLVLGGGAVNLYEMVGGYAAFSQEGIYHKQSYILKVIDRDNKILEEYKD
                     ESNPVCDAEPVRLLNDILADNLARAPLYSGGINNPLFFGDNIPVAAKTGTSQDSRDA"
     gene            complement(1939..2367)
                     /locus_tag="P5241_00700"
     CDS             complement(1939..2367)
                     /locus_tag="P5241_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRV32099.1"
                     /translation="MAAEDDDFYKHGPIDIKSIARAIYTNILHMGKTQGASTITQQLA
                     RNAFLTRTKTYARKIKEIILAFYLENQYSKDQILEFYLNQVPYGYNAYGVESASQLYF
                     NKPIQDVDIAEAAYLASLLKSPSYLSPFGPNKNKLDDRKN"
     gene            complement(2374..2535)
                     /locus_tag="P5241_00705"
     CDS             complement(2374..2535)
                     /locus_tag="P5241_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32100.1"
                     /translation="MSKDLPNPNELGNKVVSQSVKIYDRTGTTLLYNAGEVENRHIVP
                     LNTIPQVVR"
     gene            complement(2789..3010)
                     /locus_tag="P5241_00710"
     CDS             complement(2789..3010)
                     /locus_tag="P5241_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32101.1"
                     /translation="MFEREETQETITFGKSVKIADSTIKSLVYVPPEPKLYLISQGSL
                     QIDRGEIVLSGVGNNKTIEITNSGNFIIR"
     gene            complement(3095..3358)
                     /locus_tag="P5241_00715"
     CDS             complement(3095..3358)
                     /locus_tag="P5241_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRV32102.1"
                     /translation="MEINDYKNSINRDKIHFGFTLVEMLVTLSIIVIMSSILIVYSRN
                     GEKVGQLNRATERLSFDVRRMASMSMQTKQFKDSSGNLHDVCG"
     gene            complement(3367..3636)
                     /locus_tag="P5241_00720"
     CDS             complement(3367..3636)
                     /locus_tag="P5241_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32103.1"
                     /translation="MTLSKGRAMGNGDPIIAAFIGLILGMAGSIVFLLLSFIIGGIYS
                     VILLLLKKKTIKQHIAFGPILAVSAMLIFLFGHQIVASYINLLIR"
     gene            complement(3940..4302)
                     /locus_tag="P5241_00725"
     CDS             complement(3940..4302)
                     /locus_tag="P5241_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRV32104.1"
                     /translation="MSTIFFIHFMYVFLFCLGAIVGSFLLVVILRSEQEEEIVKTPSH
                     CPYCGKKLVWYELVPILSFIALKGKCKNCKHKIPFMYPAIEIVTGLLFMLVFWKFNTS
                     PLFHIALTYIPSFDAMLA"
     gene            complement(4340..4813)
                     /locus_tag="P5241_00730"
     CDS             complement(4340..4813)
                     /locus_tag="P5241_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRV32105.1"
                     /translation="MIKKYQGFTLIEMLVVVAIIGMLSSLIFGGLISSSRAKARDAKR
                     IADIANMQSVLELYYTKFGFYPEAQSDWETFAMILTSSETGIGISKIPKDPLASTGKS
                     YFYARSEDSNNLGQDYVLGAQLETKDQALDEDIDGNIFGVDCGESVGDMIYCVKP"
     gene            complement(4888..5448)
                     /locus_tag="P5241_00735"
     CDS             complement(4888..5448)
                     /locus_tag="P5241_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32106.1"
                     /translation="MTGKELLEEKILIDKINADLQTNNIDFVVYHGCECNILKNGLLD
                     IEDKYLKELDYVIVGIHSNFDLDNKSQTERIIRALENPLVNILAHPTGRLINKRDEYN
                     IDFDKILALSTKNNKILEINASPFRLDLDRHNIAKAISHNITMIINTDSHNIEQLDFM
                     KYGVAEARQGGATKNSILNTRPVIVF"
     gene            complement(5563..6144)
                     /locus_tag="P5241_00740"
     CDS             complement(5563..6144)
                     /locus_tag="P5241_00740"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV32107.1"
                     /translation="MNKELAKIFYALSYYLEMESIPFKPQAYQKAAGLLESLDENIED
                     IYKQGGLKALEDLPGIGKNIALKIEEYIKTGKIKMFEDYYRKIPVKIFDLLNINGLGP
                     KTIYNLYKYLGIKDLKDLQIAIENNKIRTVPRLGVKTEMKLKKSLDFYNQKHYNLLTS
                     KDLDHVDSDFGNLIKLSDIKGDLHIHTPRHSFV"
     gene            complement(6153..6995)
                     /locus_tag="P5241_00745"
     CDS             complement(6153..6995)
                     /locus_tag="P5241_00745"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRV32108.1"
                     /translation="MDKIFVINNLGEKEVFSPTKLYKSARRSGASKELASRVVNTISP
                     LVKNGDTTKNIYKLMKGLLLKLEKPVGIKFSLKESLRKLGPTGYPFEIFMGELFARYG
                     FQTKIEIFVKGICVDYEIDFQAVKDKTMYLSECKYHSDSGSRVDINTILIAYASFVDM
                     DQGEYCSSYKKQGGQVKRLVVTNTKFTNKAIKYASCFGVGLLGWHYPDNSGIEHLIDS
                     SRLYPITILPSMNNYLLDIFLKKGIVFCVDLLKWNEHDLTKQFHIDANIAYKILAESK
                     MLLQ"
     gene            complement(7129..7269)
                     /locus_tag="P5241_00750"
     CDS             complement(7129..7269)
                     /locus_tag="P5241_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32109.1"
                     /translation="MVNKGPIVFIEGGNEAGTMNFEIDVENHCVKVYRGLAPQSKEED
                     WQ"
     gene            complement(7297..7674)
                     /locus_tag="P5241_00755"
     CDS             complement(7297..7674)
                     /locus_tag="P5241_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32110.1"
                     /translation="MSLVDLGFTPEKISAKHIEVDKQIISWQDFSYLVVSSFYPQSYS
                     AAMFIYGPGIYGETDLKSILILSNGLEGLVIDKIPHIIKVVDINEDNSPELVVDIGSY
                     TEYYEQYTILTLENDALRLVFNE"
     gene            complement(8080..8511)
                     /locus_tag="P5241_00760"
     CDS             complement(8080..8511)
                     /locus_tag="P5241_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32111.1"
                     /translation="MDIFKNLKNDDKYYWTNHVKEKMMFYNIVPSRIKRVLRAPHRNE
                     FGIADNTFACMQQIGSKKKPQELWVMYQIRKKLKTNKQELSDLDLNILAKLGDDELEK
                     ITKLKPQIIIISSWRYPGISPKKDEVPMPEDIKKLFGLSSL"
     gene            complement(8554..9366)
                     /locus_tag="P5241_00765"
     CDS             complement(8554..9366)
                     /locus_tag="P5241_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HRV32112.1"
                     /translation="MQVYGISSAQLAYFDIFNPFQLWLIVVFITGLDVFGFVISKLFG
                     KKKGIIISSLVAGFISSTLFTISLSKQDKATSGQPNVLNWIMSSIMLANLASFVYLFI
                     LIAPLNSSLLLVLTPTLCLLLLSTFLLFWFFYYNARKHQNLTKVEDSIKTSGDLFSFS
                     KALIFACELVLIRIIAKICLVVFGEPGYIASLSLASLIGMDTIAINLAETFGKILTAK
                     RTVFIFLSVNSFNLISKVVYSFIYGSKKLAWRLLVAMLIIICCSWFGYLFIK"
     gene            complement(9379..9927)
                     /locus_tag="P5241_00770"
     CDS             complement(9379..9927)
                     /locus_tag="P5241_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRV32113.1"
                     /translation="MSGLVLPLKFLLALALGAVIGLEREAQINKFRQEYKQTPWLGSL
                     GGLRTFTLISLIGALVGVLNIYSFTTFSYIFASAFFVLLCAYYVVDALLKKATGLTTE
                     LAALLTFMIGFLIITEIIPIQLVIALGIMLSVLMSYKDKLERFAHEFPRNEWESLLSF
                     AIIALVILPFLPNKTFYLSDIK"
     gene            complement(10099..10169)
                     /locus_tag="P5241_00775"
     tRNA            complement(10099..10169)
                     /locus_tag="P5241_00775"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10135..10137),aa:Gln,seq:ctg)
     gene            complement(10215..11306)
                     /gene="dnaX"
                     /locus_tag="P5241_00780"
     CDS             complement(10215..11306)
                     /gene="dnaX"
                     /locus_tag="P5241_00780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRV32114.1"
                     /translation="MAQLVFYRKYRPQTFKEILEQQIIVQTLSNAILENKIAHAYLFC
                     GPRGTGKTSMARVFAKAINCFNRKDNEYEPCNKCESCNDINNGSSFDVIELDAASNRG
                     IDEIRNLKDNIKIKPLKSKYKIFIIDEAHQLTEAACNALLKVLEEPPEYAIFIFATTN
                     PEKMIPTILSRVQRFDFKRISLNGIINQLEKICKQENIKYEKEALKMIALSARGGVRD
                     SESILGQVATFDKEVTVQEVVDLIGNVDFYTIKNFIDLIIQNDKEQCIQFIHKLDEEG
                     FNLLEVNKGIIEYLRNLVLLNISKDLETIVAQTVTKEELEILKEQAELLDNNKIYKLI
                     LAFLQAQNQFYNSPIDSLPLELVILEQLV"
     gene            complement(11345..11683)
                     /locus_tag="P5241_00785"
     CDS             complement(11345..11683)
                     /locus_tag="P5241_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32115.1"
                     /translation="MVVVLVIYQNHKDLHKLKKENEKLEDLVETIAKQYNTNNKLNVP
                     GVSELENKKQKIMNYLDTHEMIVNDDVEQLLNVSDSLATKYLQMLEDDGKIVQIGERG
                     RYVYYIKRKV"
     gene            complement(11868..12071)
                     /gene="rpsR"
                     /locus_tag="P5241_00790"
     CDS             complement(11868..12071)
                     /gene="rpsR"
                     /locus_tag="P5241_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRV32116.1"
                     /translation="MKQCFFCQKNIDDIDYKNVDILKHFLSSQYKILPRKKTLVCAKH
                     QRVLAKAVKRARQMSLIPTTLKH"
     gene            complement(12424..12849)
                     /locus_tag="P5241_00795"
     CDS             complement(12424..12849)
                     /locus_tag="P5241_00795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRV32117.1"
                     /translation="MATKIDIRPAFLLAVITQESNLGNNVGTCNRPGDPPNKSYKVVM
                     KPSRDQIPFEQITKQLGMDPSSTPISCPMYKNGEQVGWGGAMGPAQFLPSTWLGYKDR
                     VAAITGRSPANPWNITDAFVAAALYLTDKGAGSKTYNAE"
     gene            complement(12850..13614)
                     /locus_tag="P5241_00800"
     CDS             complement(12850..13614)
                     /locus_tag="P5241_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32118.1"
                     /translation="MHDNQVKAPGNNMFLPVEVQAVNEEERAKLEKELKELDAQIAQY
                     EQQLKTTQTEKTTLQNTINKLKAKASSISLQIKSSQGNLTQIKGRIQDTQLAINQTMS
                     NIERNKQELAVLLQTLYQYKSSSDLEILLANESFQEYFDRNNNINILQDKIKTKLQDM
                     QALKTTLTEQKQDLDDQKETTQKLLSVQLLQQQELDKTKKEQEQLLSATKGKEATYQQ
                     ILSEARKKAAEIRSRIYELVGVKSKVTFGEALDIAN"
     gene            complement(13618..13761)
                     /locus_tag="P5241_00805"
     CDS             complement(13618..13761)
                     /locus_tag="P5241_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32119.1"
                     /translation="MFKKFDIIATKSNTKEQAPLSIPIRKLVIPKSVFVIGLSLILII
                     GGA"
     gene            complement(13775..14470)
                     /locus_tag="P5241_00810"
     CDS             complement(13775..14470)
                     /locus_tag="P5241_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRV32120.1"
                     /translation="MDQLHLPAKTRNVFGKKNKQLRANGMIPATVYGQQIGPFSIAVD
                     YKEFESIYKQAGDNTVIELNIDNKKTLPVLIQDVVYTPVSEQIVHIDFHAINLAEKVT
                     AYVDIEVVGENDLLKEGGNIVVAMQEVEVEALPTHLPHKIQVDISGIKNFGDTIYVKD
                     LKISKDVKVLADENNPVVTLAAPENIAEELKEMENQTSTAAAASVTEPVTSNTEQITD
                     KDNNTLTQDAEHK"
     assembly_gap    15190..15289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15570..>16578)
                     /locus_tag="P5241_00815"
     CDS             complement(15570..>16578)
                     /locus_tag="P5241_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRV32121.1"
                     /translation="IKNLAGLNVQVTDIVQEGRFKITEEGKGDRDCRVSIIPSGYGET
                     VVLRILESNITTKSLIDLGMLPEFEHDIKQSISMPSGIVISCGPTSAGKTTTMYSILS
                     LLNSPRIKIMTVEDPIEYRLPGVVQTQVNETSGYTFSVALRSFLRQNPNIILVGEIRD
                     EETAKIAIQASLTGHLILSTLHTKDTATAISRLTNFQISYSDIAASLNTLIAQRLIRK
                     LCPYCKKEISIPDNILQSITSEYNKLSPNLRQRFGKYFDVKIGNKTKMIYEPVGCEKC
                     NYTGFWGQSGIFEILIIDEELRNIIVKGADINILQKEVQKRTLTLKQDGIIKVLQGVT
                     N"
BASE COUNT         5790 a         2873 c         1926 g         5889 t
ORIGIN      
        1 aacctttgaa cagttgattt tcgtagcaaa atataattag ggaatacaat gtatcattta
       61 ttatcaatat attatgaata tatcatattt ataaaaataa aacaatagat gatgggagat
      121 ataataattt taattgggta atgttttgta attttaaaaa tgtatatttt atttatatat
      181 tgttatagca tcttctataa tatccaaagc actatcttgt aaatgaactc gtatttcatt
      241 ttgtggttgt aaagtgttat aataaatcat aataccatat gttttaatat cttgattttg
      301 agaaattaaa ttcatttcac caatatattg attgtttaaa tagacagatt gttttattaa
      361 atctgtcccc cctgttacct gtacttgtaa ttgtgtatca ttgttccaaa tttgtccatt
      421 ttgaacattc aaaaatttca tttgtaattc tttagagtaa gaagtagtat tggtggggat
      481 agtagaaaca tttgacccat aatcaacatc atacacatct aaactcattg gtttattata
      541 tactgtgtta aaatcttgaa tattattttg ggctcaagca atcactggac cttctcaatt
      601 ataaaattga gaatcattgg caggattgcg tggaatagga cctaagggat tgtttttatc
      661 aatataaaaa agaatcgagt gaatttgtaa agagccccca atactattta tatattgccc
      721 atttaacatt ggtttagata ttgcagtaat ttgaggtttt gtgaaatatt ctatattatt
      781 atttttagta atagcatact gcataaattc atgccaagct ggagcagcaa ccaaacctcc
      841 tgttactgat ccctggtata ttggtgaata atcattatta cctactcata caccagtaac
      901 aatattggga gtataaccaa aaattcaagc atctcgtgaa tcttggcttg taccagtttt
      961 ggcagcaact ggaatattat ctccaaaaaa taacggatta ttaattcctc cactataaag
     1021 tggggcacga gccaaattat cagccaaaat atcatttaat aatcttactg gctcagcatc
     1081 acaaactgga ttagattcat ctttatattc ttctaaaatt ttattatctc tatctataac
     1141 ttttaaaata tagctttgtt tatgataaat accttcctgg ctaaatgctg catatccacc
     1201 aaccatttca tacaaattta ctgccccgcc tccaagcacc aaaggcaatc cataataatt
     1261 ggcatcttgt gttaaagtag ttataccaaa agattttgcc gtattaattg tatctgtaat
     1321 acctgctaaa tataatactt taactgaggg aatattcaaa gattgtgcca aagagtttct
     1381 taaatttatt ggacctctaa attttttatc ataattttta ggagtatagg gattttgtgg
     1441 atctgttgaa aaattagttg gtaaatcaaa taatatagta ttatctgtat agcctttttt
     1501 aaataatgtt aaatacgcaa aaggcttaaa agaagaacca ggctggcgta atccaaacac
     1561 agcattaaaa ttaccttcat tttctaaatt aaaaaaatct cgtgacccta ccatagctaa
     1621 aatttgtcca gttttaggat cttcggaaag caaggccata ttttttactt tataatctcg
     1681 ttcattcaaa tctgcatatt tttttactac ctcctcggct ttagcctgta aatctaaatc
     1741 caatgtacta ataactgtaa gcccaccact ttgaatggct tcttcaccat attttgctac
     1801 caattcttgc tctataaaat tcacaaaatg aggggcaatc attctaatat tttttgccac
     1861 aaaaactact ttttcttttt ttgccttatc tgcttcttgt ttagtaatat atcctaaatt
     1921 actcattcta tcaataactc aatttttacg atcatctaat ttatttttat taggtccaaa
     1981 aggagataaa taactcggtg attttaataa actcgctaaa tacgctgctt cggctatatc
     2041 aacatcttga atgggtttat taaaatataa ttggctggct gattctacac cataagcatt
     2101 atagccatac ggtacttgat ttaaataaaa ttctaaaatt tgatctttgg aatattgatt
     2161 ttctaaataa aatgccaaaa taatttcttt aatttttcgg gcatatgttt ttgtgcgagt
     2221 caaaaaagca tttcttgcca attgctgagt aatagtagat gctccttgag ttttacccat
     2281 atgtagaata ttagtataaa tggcacgagc aatagattta atatcaatag gcccatgttt
     2341 ataaaaatca tcgtcttcgg cagccaaagt agctcagcgt acaacctgag gtattgtatt
     2401 caaaggcaca atatgacgat tctcgacttc gccagcatta tacaataaag tagtacctgt
     2461 acgatcatat attttaacac tctgggagac aactttgttg cctaattcat taggattagg
     2521 caaatcttta gacaaataaa aaatcaccaa tattgctatc acaatacaga tacaaaataa
     2581 tcctaaaagt tttttaaaaa tatttctttt tcgtgccatt ataaattata aaataaatta
     2641 cagtgataat tctccttttt ttctaaaata tattatataa catttaaaaa taataatgtt
     2701 gagtttatat tcaataaaaa taaaatctca atattaaccc aaaaaattgt tagttatata
     2761 tctatgtcaa cattttgttt aaaatatatt aacgaattat aaaattacca gaattagtta
     2821 tttctattgt tttattatta ccaacaccag acaaaactat ttccccccta tctatttgca
     2881 aagagccttg actgataaga tataattttg gttctggagg aacatatact aaacttttta
     2941 tagtggaatc tgcgatttta acactttttc caaacgtaat agtctcttgt gtttcctcac
     3001 gttcaaacat taaatttccc ttatcttgtc ctgtttcaca aaaatcacta aaaataatat
     3061 aggaattact accaggatcg ccaaaataaa tacctcaacc acacacatca tgcaaattac
     3121 cagaactatc cttaaattgt tttgtttgca tagacatact agccattcta cgaacatcaa
     3181 aacttaatct ttctgttgcc ctatttaatt gcccaacttt ttcaccattt ctcgaataca
     3241 ctatcaaaat tgatgacata ataacaataa tagacaatgt aactaacatt tctaccaatg
     3301 taaatccaaa atgtatttta tctctgttta tactattttt ataatcattt atttccatat
     3361 attaatttag cgtatcaata aatttatata actcgccaca atttgatgac caaataaaaa
     3421 tatcagcata gccgaaactg ccaatatcgg accaaaagca atatgctgtt ttatagtttt
     3481 cttttttaat aacaataata taacagaata aataccacca attataaaag ataacaacaa
     3541 aaatactatg gagcctgcca tacctaaaat caaaccaata aatgccgcaa taatcggatc
     3601 tccattaccc atagccctac ctttagacaa agtcactatc aattgaataa cgcctcaagc
     3661 tataatcata cctaacaaat tatcccaaat accagtaaac caaggattta ttaataattc
     3721 ataccctccc aaaaactgac gaaagctcaa cccaaaaggg acaatacttt ttgtatatag
     3781 tgcagaatat ttaccaataa gaaataaaga tagctcacct acaaaaccta ctaataaagc
     3841 aatatataaa aatttatcta aaacaacaaa attacgaaaa tcatatatag atattaataa
     3901 taaaatagag acataaaata atcataaaaa caaagtcaat caagcaagca tcgcatcaaa
     3961 tgacggaata tacgttaaag caatatgaaa caaaggagaa gtattaaact tccaaaatac
     4021 caacataaat agtaaccctg taacaatttc tatagctgga tacataaacg ggatcttgtg
     4081 tttacaattt ttacacttac cttttaaggc aataaaactt aatatcggga ctaactcata
     4141 ccaaactaat tttttaccac aataaggaca atgagacgga gttttgacga tttcttcttc
     4201 ttgttcgcta cgtaaaatta ctaccaaaag aaaacttcca acaatagcgc ctaaacaaaa
     4261 taaaaataca tacataaaat gaataaaaaa aatagtagac ataaatccta agtttaataa
     4321 atttttaaat taaaacaatt tatggtttta cacaataaat catatctcca acagattccc
     4381 cgcaatcaac accaaatata ttaccatcta tatcttcatc cagagcttga tcttttgttt
     4441 ctaattgagc gcctaataca tagtcttgcc ctaaattatt tgaatcttca gacctagcat
     4501 agaaatatga ttttcctgta ctcgccaagg ggtctttggg aattttagaa atacctatac
     4561 ctgtttctga actagtcaaa atcatagcaa aagtctccca atcagattgc gcctctggat
     4621 aaaacccaaa ttttgtataa tacaattcca aaacactttg catattagca atatcagcaa
     4681 ttcttttggc atctctggct ttggctcttg aactactaat caacccacca aaaatcaaag
     4741 agctaagcat accaataata gccacaacaa ctaacatttc aattaaagta aaaccttgat
     4801 attttttgat cataatacaa atttaaaagt ttattaaatt atgtgattat taataatgaa
     4861 tatgtagata gtatatcata aattctatta aaaaacaata acaggtcgcg tatttaatat
     4921 actatttttg gtggcacctc cttggcgtgc ctctgccaca ccatatttca taaaatctaa
     4981 ctgttctata ttatgagaat cagtattgat aatcattgtg atattatgac taatggcttt
     5041 ggcaatattg tgtctatcca aatccaaacg aaatggcgaa gcatttattt ctaatatttt
     5101 attatttttt gtggatagag ctaaaatttt atcaaaatca atattatatt cgtcacgttt
     5161 atttattagt ctgccagttg ggtgtgctaa aatatttact aatggatttt ccaatgccct
     5221 aattattctt tcagtttgac ttttattatc caaatcaaaa tttgaatgaa tacctactat
     5281 aacataatcc aattctttta aatatttatc ttctatatct aataaaccat ttttcaaaat
     5341 attacactca caaccatgat ataccacaaa atctatatta tttgtttgca aatcagcatt
     5401 tattttatca atcaaaattt tttcttctaa taattcttta cctgtcaaac catactctat
     5461 ttttaaatat tctgtatggt tagaaatacc gatatattta tagccacgtt ttatagattc
     5521 gtggatataa ttatatattt gtttttcttc ttcatctggt aatcatacaa aactatgtct
     5581 tggtgtatga atatgtaaat ccccttttat atcagacaat tttattaaat taccaaaatc
     5641 actatctaca tgatcgagat cttttgatgt taacaaatta taatgttttt gattataaaa
     5701 atccagagat ttttttaatt tcatctctgt ttttacacct aaccgtggta ctgttcttat
     5761 tttattattt tctatggcta tttgtaaatc ttttaaatct tttataccca aatatttata
     5821 taaattataa attgtttttg gccccaaacc atttatattc aacaagtcaa aaatttttac
     5881 tgggatttta cgataataat cttcaaacat ttttattttc ccagttttga tatattcttc
     5941 aatttttaaa gcaatatttt taccaatacc tggcaaatct tccaatgctt ttaatcctcc
     6001 ttgcttataa atgtcttcaa tgttttcatc taaactctcc aaaagtcctg cagctttttg
     6061 ataagcctga ggcttaaaag ggatagactc catttcgaga taataagaaa gagcataaaa
     6121 gatttttgcg agctccttat tcatattata ctttattgta ataacatttt actctcagcc
     6181 aaaatcttat aagctatatt ggcatctata tgaaattgtt ttgtcagatc gtgttcattc
     6241 cactttaata aatcaacaca aaacactata ccttttttta aaaaaatatc taataaataa
     6301 ttattcatcg atggcaaaat agttatagga tataaccgcg aactatcaat taaatgctca
     6361 atgcctgaat tatctgggta gtgccacccc aataaaccca caccaaaaca tgaggcatat
     6421 tttatagctt tattagtaaa ttttgtatta gttactacga gtctttttac ttgaccccct
     6481 tgttttttat aagaactgca atattcacct tgatccatat ctacaaaact cgcataagca
     6541 atcaaaatag tatttatatc tactctgcta cctgaatcag aatgatattt acactctgat
     6601 aaatacattg ttttatcttt cacggcctga aaatctattt cgtaatccac gcatatacct
     6661 ttgacaaaaa tttctatttt tgtttgaaac ccataacggg caaacaattc acccataaaa
     6721 atctcaaaag gataaccagt tggacctaat tttctaagag attcttttaa actaaattta
     6781 ataccaaccg gtttttctaa cttcaaaagc aagcctttca taagcttata aatatttttc
     6841 gttgtgtctc catttttaac taatggtgaa atagtattaa ccactcgact cgctaattct
     6901 tttgaagccc cactacgtcg agcgctttta tatagttttg ttggggaaaa tacttctttt
     6961 tctcctaaat tatttataac aaaaatttta tccataaaaa attctaaaaa tattattata
     7021 gtacaatcat aaactataat cttgtactaa actgataaaa atattttatt ccatactttt
     7081 aaatacaaat tgatgctcaa ttggctgtaa ttcatatatt acagaagttc attgccaatc
     7141 ttcttctttg ctttgaggcg ccaatccacg atatacttta acacaatgat tttctacatc
     7201 aatttcaaaa ttcatagtgc cggcttcatt acctccttct ataaaaacaa taggaccttt
     7261 atttaccatc tgttgggtat ctttatcttt atctattcat tcattaaata ctaatcttaa
     7321 tgcatcattt tccagcgtta aaatagtata ctgctcataa tattctgtat atgaaccaat
     7381 atcaacaacc aattcaggac tattatcttc attaatatcc actactttga taatatgagg
     7441 aattttatca ataaccaaac cctccaggcc attagacaaa attaatattg attttaaatc
     7501 tgtttctcca taaatacctg gcccatatat aaacatagca gcactataag attgtggata
     7561 aaaagaagaa acaactaaat aagaaaaatc ctgccacgaa ataatttgtt tatctacctc
     7621 tatatgttta gcacttattt tttcaggtgt aaaacccaaa tctactaatg acactgtagc
     7681 tggatcccat tgtcgtattt taaattctat tgttgtcttt tcttttaaat ataatgcccc
     7741 aaatcaacaa aaagccagta taataataca aacaacataa gctccatata ttgtaaatcc
     7801 tgttttttta ttcatattgt ttttattaaa attttaatta aatttatata tattaaaaag
     7861 cttttaataa accaccatta ttaaataagt atagccataa cctgctatat tattatataa
     7921 taattatagc acttcttgat ataaaaatca agtactaaaa aatatattaa aattaagtta
     7981 atattataat ataatacaaa gtatacaaca gctacaacta ccatcaaaac aaagtatcgt
     8041 ccaaaaataa attactatga gatatggggt cttttattct tataaagaag acaaaccaaa
     8101 taactttttt atatcttctg gcataggtac ctcatctttc ttaggactta taccaggata
     8161 acgccaacta ctaataatga taatttgtgg ttttagctta gtaatttttt ctaattcatc
     8221 atcacccaat ttagccaaaa tgtttaaatc taaatctgat aattcctgtt tattggtttt
     8281 taattttttt cttatctgat acataaccca caattcctga ggttttttct tagaaccaat
     8341 ttgttgcata cacgcaaaag tattatcagc aataccaaac tcatttctat gtggtgcacg
     8401 caaaactcgt tttatacggc tgggcacaat attataaaac atcatttttt ctttgacatg
     8461 attggtccaa taatatttat catcattttt tagattttta aaaatatcca taatccaatt
     8521 atatctaata taatgtatag ctttgtactt aaattattta ataaataaat atccaaacca
     8581 actacaacaa ataattatta acattgccac taacaatctc caggctaatt ttttactgcc
     8641 ataaataaaa ctatatacga ctttgcttat caaattaaaa gaatttacag acaaaaatat
     8701 aaacacagtc cttttggcag ttaatatttt cccaaacgtt tcggctaaat ttatagctat
     8761 ggtgtccata cctattaatg atgccaaaga caaactggct atatatcctg gctcgccaaa
     8821 aacaactaaa caaatcttag ctataattct aatcaaaact aattcacaag caaaaatcaa
     8881 tgccttgcta aatgaaaaca aatcaccact tgttttaata ctatcctcta cttttgttaa
     8941 attttgatgt ttccgagcat tataataaaa aaaccaaaat aacaaaaaag tagataataa
     9001 taataaacat aatgttggcg tcagaactaa taataacgac gaattaagag gggctattaa
     9061 aataaataaa tacacaaaac ttgctaaatt tgccaacata atactagaca taatccaatt
     9121 aagtacattc ggctgaccac tggtggcttt atcttgttta gacaaagaaa ttgtaaaaag
     9181 agtagatgat ataaaaccag ctactaaact tgaaattatt ataccttttt tcttaccgaa
     9241 taatttagat ataacaaaac caaaaacgtc taagcctgtt ataaacacaa caataagcca
     9301 caattgaaaa ggattaaata tatcaaaata ggctaactgg gcagagctaa taccatatac
     9361 ttgcaatata ttaacaagtc attttatatc actcaaataa aatgttttgt taggcaaaaa
     9421 tggtaaaatt accaaagcaa taatggcaaa actcaacaag ctctcccatt catttcgtgg
     9481 aaattcatga gcaaaacgtt ctaatttgtc tttatatgac atcagaacac tcaacataat
     9541 ccccagagca ataactaatt gtattggaat aatctctgta ataatcagaa accctatcat
     9601 aaatgttaac aaagcagcta actctgttgt caaccccgta gcttttttta ataatgcatc
     9661 aactacataa taagcacaca ataaaacaaa aaatgcacta gcaaaaatat aagaaaaagt
     9721 tgtaaacgaa taaatattta aaactcctac taaagcccca ataagagaaa tcagagtaaa
     9781 cgtacgcaac cctcctaaac tgcctaacca aggggtttgt ttatattctt gtctaaattt
     9841 attgatttga gcttctctct caagtcctat tacagcccct aaagccaaag ctaataaaaa
     9901 ttttaacggc aaaactaatc cactcataat attatatttg tatctattta tttttataaa
     9961 gatttataat aaattaaaat tatatcaaat gtgttttaac ttaataatat attatattta
    10021 ttatttcttt gtattttaaa aattttttct tatgccccta aactactaat tacaaattat
    10081 tcagctcttt ctacaaggct gcggggcctg gactcgaacc aagataagcc gctccagagg
    10141 cgactgtcct accattagac gaccccgcac aatattgtgt tcttattatg ttacaaaacc
    10201 tttatattat acctttacac caattgttct aatattacta actctaatgg caagctatct
    10261 attggagagt tataaaactg attttgagct tgtaaaaaag ccaagatcaa tttataaatt
    10321 ttattattat ctaataattc agcctgctct tttagaatct ccagctcttc tttggttaca
    10381 gtctgcgcca caatagtttc taagtcttta gatatattta acaataccaa gtttcttaaa
    10441 tattcaataa tacctttgtt aacttctaat aaattaaaac cttcttcatc caatttatga
    10501 ataaattgga tacattgttc tttatcattt tggataatca aatcaataaa atttttaatt
    10561 gtatagaaat ctacattgcc tatcaagtcg acgacttctt ggactgttac ttctttgtca
    10621 aaagttgcta cttgccccaa aatagattca gaatctcgca ctccccctcg agcgcttaaa
    10681 gcaatcattt ttaatgcttc tttttcatat ttaatatttt cctgtttaca aattttttct
    10741 aattgattaa ttataccatt caatgaaatt cttttgaaat caaatctttg aactcgtgac
    10801 aaaatagttg gtatcatttt ttccggattg gtagtagcaa aaataaaaat agcatactcg
    10861 ggcggttctt ccaaaacttt caacaaagca ttacaagcag cttcggttaa ttgatgtgcc
    10921 tcatcaataa taaaaatttt atatttactc ttcaatggtt ttattttaat attgtctttt
    10981 aaattacgta tttcatcaat acctctatta gaagcagcat ctaattctat aacatcaaaa
    11041 gaactaccat tgtttatatc attgcatgat tcgcatttat tacatggctc atattcatta
    11101 tcttttctat taaaacaatt tatagctttg gcaaaaaccc gagccattga agtcttacct
    11161 gtaccccggg ggccacaaaa taaataagca tgagctattt tattttctaa aatagcattg
    11221 cttagtgttt ggacaattat ttgttgctcc aaaatttctt taaatgtttg gggcctatat
    11281 ttgcgataaa aaactaattg agccatttaa tcaacttaaa actacgttta atatatcccg
    11341 tttattaaac ttttcgttta atgtagtaaa cataacgacc acgctcacct atttgaacaa
    11401 ttttgccatc atcctccaac atttgcaaat actttgtggc taaactatcc gagacattca
    11461 ataattgttc aacatcatca ttaacaatca tttcatgggt atctaaataa ttcataattt
    11521 tttgtttttt attttccaac tctgatacgc ctggcacatt taatttatta ttagtattat
    11581 attgtttagc tattgtttcg actaaatctt ctaatttttc attttctttt tttaatttat
    11641 gtaaatcttt gtgattttga taaatcacca atactactac cattgttcaa accacaaaaa
    11701 ttaaaattat aaacgccata atatattttg aatttaattt attaaacgaa aaatatagaa
    11761 taattattta aataaaaata tattataaat caatttcaca aatcattaaa atcacaaaat
    11821 atatcaaatg tccattaata tacaaaaacc agttctatat tttggtttta atgttttaat
    11881 gttgttggta tcaaagacat ctgtctagct cttttaacag ccttggccaa aaccctttgg
    11941 tgtttagcac aaactaatgt ctttttccta ggaagaattt tatactggct tgataaaaaa
    12001 tgttttaata tatcaacatt cttataatct atatcatcaa tatttttttg acaaaaaaag
    12061 cattgtttca taatttgagt ttatttatta aacgaaaatt atattgtatt cgttatataa
    12121 tttgagttta tttattaaac ggaaattaaa ttgtattagt tatatattga gaatttaatt
    12181 tattaattaa atgaacaata tattgtattc gttatataat ttgagtttat ttattaaacg
    12241 aaaattatat tgtattagtt atataatttg agtttaattt attagatgta aaaaataaaa
    12301 ttatttcgct ttattaattg cttcaatatc tgcctgatat cttgtggcga ttgccattac
    12361 attgtcgcca taaaatctat atgcattatt tactgtaccc gcaaaataaa tcattgccgc
    12421 tcgtcattca gcattatatg tttttgaccc tgctccttta tctgttaagt ataaagccgc
    12481 tgctacaaaa gcatcagtaa tattccaagg atttgctggg cttcgaccag taatagcagc
    12541 aacacgatct ttatatccca accaagtaga gggtaaaaat tgtgcaggcc ccatagctcc
    12601 tccccagcct acctgttctc catttttata cattgggcaa gaaataggcg tggaagatgg
    12661 atccatccct aactgtttcg ttatttgctc aaaaggtatc tggtctcgac taggcttcat
    12721 tactactttg taacttttat ttggaggatc gcctggccta ttacaagtcc caacattatt
    12781 tcccaaatta gattcttgag ttattactgc caataaaaat gccgggcgaa tatcaatttt
    12841 agttgccact caattagcta tatctaaagc ctcaccaaat gtaactttcg attttacacc
    12901 aactaattca taaattctac ttcttatttc tgctgccttt tttcttgctt cgcttaatat
    12961 ttgctgataa gttgcttctt tacccttagt agcgcttaat aattgctcct gctctttttt
    13021 agtcttatct agttcttgct gttgtagtaa ctgcacagat aataattttt gtgttgtctc
    13081 tttttgatcg tccaaatctt gcttttgttc tgttaaagtc gtttttaatg cttgcatgtc
    13141 ctgtaatttg gttttaattt tgtcttgtaa aatatttata ttattgtttc tatcaaaata
    13201 ttcttgaaaa ctctcattag ctaataatat ctctaaatca ctcgaacttt tatactgata
    13261 taatgtttgc agcaaaactg ccaactcttg tttatttctc tcaatattgg acattgtttg
    13321 atttattgcc aactgtgtat cttgtattct acctttaatc tgcgtgagat tcccttggct
    13381 tgatttaatt tgtaacgaga tagatgacgc tttggctttt aatttattaa tggtattttg
    13441 taatgttgtt ttctcagttt gagtagtttt aagttgctgt tcatattgcg ctatctgagc
    13501 atctaactct tttaattctt tctctaactt agcacgttct tcctcattga cagcttgcac
    13561 ctctacaggt aaaaacatat tattccccgg tgcttttact tgattatcat gcaagtatca
    13621 agcgcctcca attattaata tcaaagacag cccaattaca aaaactgatt tgggtataac
    13681 caacttccta ataggtatac tcaaaggcgc ctgctctttt gtattggatt ttgtagcaat
    13741 aatatcaaat tttttaaaca taaaatatat ttaattattt atgttctgcg tcttgtgtta
    13801 aggtgttatt atctttatct gttatctgct cagtattaga agttactggt tctgtgacag
    13861 aagcagcagc cgcagtagaa gtctgatttt ccatttcttt taattcttct gcaatatttt
    13921 ctggggcagc taatgtcact actggattat tctcatcagc taaaactttt acatctttac
    13981 taatttttaa atctttaaca tatattgtgt ctccaaaatt tttaataccc gaaatatcaa
    14041 cttgaatttt gtgtggtaaa tgggttggca aagcttctac ttccacttct tgcatagcca
    14101 caactatatt gcctccttct tttaacaaat cattttcgcc tacaacctct atatctacat
    14161 aagcagttac tttttcagct aaattaatag catgaaaatc tatatgcaca atttgttctg
    14221 atactggcgt gtacactacg tcttgtatta acactggtaa agttttttta ttatcaatat
    14281 ttagttctat taccgtgtta tctcctgctt gtttatatat agattcaaac tctttataat
    14341 ccacagctat agaaaatggt cctatttgtt gtccatatac agttgctgga atcataccgt
    14401 tggcacgtaa ttgtttattt ttcttgccaa atacatttct tgtcttggct ggtaaatgta
    14461 attgatccat tattcaaatt tatttattat aaatttacta ctctttatac actaataata
    14521 gtatatcaaa ttttaaattt aaaagcaacc cttatatata aaaatagaac aagacctttc
    14581 ggtcttattc tatcctgcag ttctccttta tgtttttaca ctatcatatt atttaataaa
    14641 aatcaagtat catcatatac caaatgtatt attgggtaat cattatttta ctttattgga
    14701 gttagtataa aaattaaaaa actctagtaa tctgcaggca agatcctccg cacaaagacg
    14761 gagattaaga gaattactga ggtgcaaccc agtataacac cagattgtcc atctacataa
    14821 gctctggcag aaccttattc aataaaaaaa ggtctgttgc ctacaggtta ctagagtttt
    14881 tatatttaat tatacaataa attatacaat ggcaagtata gcatattttt attattttgt
    14941 caatacccca gcaagttatc atacaaatat aataaacacc ctaatataat caaaagaaca
    15001 taaatatcta agaaaaaaca attaagagga acatcaaaat ataataaata ctttaatatc
    15061 agcaaaaggg caaacatatc taagaagaaa caaccaagag gaacatcaaa aatatctaaa
    15121 aaacaactaa gagaaacacc aaaaatataa taaacaccct aatataatca aaagaacata
    15181 aatatctaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tttaatatca
    15301 gcaaaagggc aaacatatct aagaagaaac aaccaagagg aacaccaaaa atataataaa
    15361 tactttaata tcagcaaaag ggcaaacata tctaagaaga aacaaccaag aggaacatca
    15421 aaaatatcta aaaaaacaac taagaggcac accaaaatat aataaacacc ctaatataat
    15481 caaaagaaca taaatatcta agaaaagaca tcaaaatata atatctatcc aaattttaca
    15541 atatctccta ctactcgttc taattcttct cagttagtaa caccttgtaa tactttaatg
    15601 atcccatctt gtttcaaagt taaagttctt ttttgtactt ctttctgtaa tatatttata
    15661 tcagctcctt tgactataat gtttcttaat tcttcatcta taatcaaaat ttcaaaaata
    15721 ccactttgcc cccaaaaccc agtataatta catttttcac accctactgg ttcatatatc
    15781 atttttgttt tattgccaat ttttacatca aaatatttac caaatctctg acgtaaatta
    15841 ggagataact tattatattc tgatgtaata ctttgcaata tattatctgg tatagatatt
    15901 tcttttttgc aataaggaca taattttctt attagtctct gggcaatcaa agtattcaaa
    15961 ctcgcagcta tatctgaata agagatctga aaattcgtaa gtcgagatat agcagtcgca
    16021 gtatctttag tatgtaaagt agaaagaatt aaatgcccag tcaaagatgc ttgtatagca
    16081 atttttgcag tttcttcgtc gcgaatttcc cctactaaaa taatgtttgg attttggcgc
    16141 aaaaaacttc ttaatgccac agaaaatgtg tatcctgatg tttcgttaac ctgtgtttgc
    16201 accacgccag gtaatctata ttctatagga tcttcaaccg tcataatttt aatacgcggg
    16261 ctattcaaaa gagataaaat tgaatacatt gtagtagttt tacctgctga agtaggacca
    16321 caactaataa caatacctga gggcatacta atagattgtt ttatatcatg ctcaaattct
    16381 ggtaacatac ccaaatctat taatgatttt gttgtaatat tagattctaa aatacgcaat
    16441 actactgtct ctccataacc actaggtata atagacaccc ggcaatctct atcacccttg
    16501 ccttcttcag tgatcttgaa cctgccttct tgaactatat cagtaacttg cacattcaat
    16561 cctgctaaat ttttaata
//
LOCUS       DAOTJA010000006        15796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_4149_length_15796_cov_6.154755, whole genome shotgun sequence.
ACCESSION   DAOTJA010000006 DAOTJA010000000
VERSION     DAOTJA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15796
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_15796_cov_6.154755"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5241_00820"
     CDS             <1..275
                     /locus_tag="P5241_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32122.1"
                     /translation="MCTKDTLSTFDYISVNVNSNITTPPSSTTTSSATSSTTSSTTTT
                     TKLLESGFCGVSTYNFCTRHSDCIISGCSGQVCQSKTEAELPTTCE"
     gene            723..1061
                     /locus_tag="P5241_00825"
     CDS             723..1061
                     /locus_tag="P5241_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HRV32123.1"
                     /translation="MVSEQPAHKTMFITSKEYSGNLGGLKGADDICQYHAKLGKLSGT
                     YKAMLSDASYSAKARLTLADIPYRNVKGELIYASGLEIFDEKHKIGRVASDEFGGPAY
                     DYTGRGSNSF"
     gene            2056..2181
                     /locus_tag="P5241_00830"
     CDS             2056..2181
                     /locus_tag="P5241_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32124.1"
                     /translation="MPTIRPVFKNGEKYHIISRSIDERCIFKETKDYQRFARNLY"
     gene            2251..2601
                     /locus_tag="P5241_00835"
     CDS             2251..2601
                     /locus_tag="P5241_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV32125.1"
                     /translation="MLLRAKYDREILVKIISVCLMPNHIHLLLEQVTNDGISQFMKKL
                     AGGYACYFNNKYKRCGHVFQGRFKVQHIQTLDTLFNTACYIHTHLAISMIRKQKNYKK
                     IDQKKIIQVIEYYK"
     gene            2904..3263
                     /locus_tag="P5241_00840"
     CDS             2904..3263
                     /locus_tag="P5241_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32126.1"
                     /translation="MENINSQHDNVKPFKRFAKWFLLIIIAIIIIYIVIIFVSPQNRE
                     WLSMLIGQKLDQRSLDNYKKQIQSDKYGGTTPLETYKMFVEALKQQDIDLAVKYFVQG
                     KQGEYKKFFEAIKQEGK"
     gene            3270..3449
                     /locus_tag="P5241_00845"
     CDS             3270..3449
                     /locus_tag="P5241_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32127.1"
                     /translation="MMADVSTSKNNTRGSQQGPNAYQLDIVDSNNFLVTTVYIVIPCD
                     INGQPISDIWKIESF"
     gene            3603..3791
                     /locus_tag="P5241_00850"
     CDS             3603..3791
                     /locus_tag="P5241_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32128.1"
                     /translation="MAYDMNTTHPELTKLIIDKYNTQTSKYKIDEGFKAWIVKGSIDE
                     DLGTRSLNHFFDPIYNRT"
     gene            3876..4391
                     /locus_tag="P5241_00855"
     CDS             3876..4391
                     /locus_tag="P5241_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32129.1"
                     /translation="MAFLASLAYSNKSVLESDATQTYQRAIYEYIKGNKAQAFYALGH
                     ILHLIEDLTVPAHTRAEPHSNLIVSTYENSAAQLTASDYTQIYNKINYDNLAKRDSLN
                     EYFDSVAVYTNTHFFSDGTILDYATYARPEVIYQDIIETNIEGIKKYYLEAQDPETQE
                     IYRLAVRGNLS"
     gene            4512..4970
                     /locus_tag="P5241_00860"
     CDS             4512..4970
                     /locus_tag="P5241_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32130.1"
                     /translation="MFFRDVEKYKNDPNFIKQMETKFGIGYVMKVLGDAVDLIKQMFI
                     GQPDVILNSSTTVLLDTQGVVTTITTQNNSTATQSSTITPNIPTATNKTTATTTKATT
                     TTKKVTTTTKKTTTTTVKKVTTTTAQPIVRCSFVYGGNSINTSMIFNEIA"
     gene            5019..5273
                     /locus_tag="P5241_00865"
     CDS             5019..5273
                     /locus_tag="P5241_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32131.1"
                     /translation="MSGNRINISNWQIVDKNGNIKIVIPNNTWIDSQAFYLLERSDDA
                     TIPYLSANLIYTGALVNNNEELQLFDSNCNLIDRVAANPD"
     gene            complement(5375..5587)
                     /locus_tag="P5241_00870"
     CDS             complement(5375..5587)
                     /locus_tag="P5241_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32132.1"
                     /translation="MGISTGLVVVEVVVEDVVEVVVSVSKLLVVVVVELIVVLVVVVL
                     LLLLYVPPPLLFVSLKVASTPTLFSA"
     gene            5574..6266
                     /locus_tag="P5241_00875"
     CDS             5574..6266
                     /locus_tag="P5241_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRV32133.1"
                     /translation="MDIPIEPIVFSEVQISGETADDEFIELYNPLDHDIDLTGYSIKK
                     LTDNNLEKKTNLVSDDNISGKIIQSHGYFLIVHAKEGSINQYKGTTSPDLYFNSSSYS
                     IVPNNTLILYDSLNREIDRIGWTNNEAVDPICEGDCLLNINDFQSFERKAKSSSTKDL
                     MQDGLDKTFGNSFDSNNNIQDFIIRDISEPQNSFSIKEPYTPENISELIIPTPTGFTA
                     VLNPQDKTMDFT"
     gene            6486..6632
                     /locus_tag="P5241_00880"
     CDS             6486..6632
                     /locus_tag="P5241_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32134.1"
                     /translation="MSDDLVVSIIGPYFINNYFDVIEYTNDENNYIILKGRAKRNFST
                     QTYC"
     gene            6867..>7084
                     /locus_tag="P5241_00885"
     CDS             6867..>7084
                     /locus_tag="P5241_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32135.1"
                     /translation="MQTSDFDQANQNNFIILRTTVTGNIPAFDPQRHYYTYKEFINED
                     ITTTTSSTTSTTIPAISSTTTTTDTTQPP"
     assembly_gap    7085..7184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7185..7512
                     /locus_tag="P5241_00890"
     CDS             <7185..7512
                     /locus_tag="P5241_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32136.1"
                     /translation="DTTTSTSTSSTSTTTSSTATTTDTTQPPDTTTSTSTSSTSTTTS
                     STTTTTCTTQPPDTTTSTSTTTSTTTTTAPKVYTNPMVDILDFYTDLEGYVRIRVRFL
                     ENVIMK"
     gene            7675..7803
                     /locus_tag="P5241_00895"
     CDS             7675..7803
                     /locus_tag="P5241_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32137.1"
                     /translation="MQNQVVDFYVSSNTCGYALMKDNLTTDDWVNFSGVQGSYGSV"
     gene            8271..8834
                     /locus_tag="P5241_00900"
     CDS             8271..8834
                     /locus_tag="P5241_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32138.1"
                     /translation="MFIVSAIVTQGFTYIPRLGGVKNYPATKKAMEFLSSQIDQAKQM
                     GQLDPTVQIELANAKIQNGVIVLTVKIGGQDLDAYITKDGSLFFPQAFNLNPQVEKFS
                     VKKTDVVDVKLFTMSYCPFGNEAETAIIPVAKLLNGYVDIIPHYVIYSQYPSPELYSE
                     YCLDKEAKYCSMHGIQELKQDVRELCI"
     gene            8934..9197
                     /locus_tag="P5241_00905"
     CDS             8934..9197
                     /locus_tag="P5241_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32139.1"
                     /translation="MKIDTNKIETCEKEEASVLLDTEVQLNNTLQVAGSPMLFINETE
                     YTGSRTPEAYKNAICDAFTKSKRPKVCDTKLSADVGQVSGSCK"
     gene            9549..9620
                     /locus_tag="P5241_00910"
     tRNA            9549..9620
                     /locus_tag="P5241_00910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9581..9583,aa:Ile,seq:gat)
     gene            9787..9857
                     /locus_tag="P5241_00915"
     tRNA            9787..9857
                     /locus_tag="P5241_00915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9819..9821,aa:Ala,seq:tgc)
     gene            10367..11896
                     /gene="rny"
                     /locus_tag="P5241_00920"
     CDS             10367..11896
                     /gene="rny"
                     /locus_tag="P5241_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRV32140.1"
                     /translation="MQNIHFFIIGASCLLLGVILGYLIRHFIGVLKSGSTEAKLQQKI
                     QSTKEEAKIIILKAKEEAAKIITNAQAEEKERKSQLDQLESRLLKREELFDQKQNELE
                     QERKKLMGDIEKVKIIKEELAHAKEIEDKRLEQIANMSKEDAKSILVKHYEDEHKEEL
                     LKMLQDFEQVKKEEVEKRVEDIIINTIQRYGHGNASETMISTVAIPSEDMKGRIIGKE
                     GRNIHHFEKVSGVEVIIDDSPDIITLSSFNPIRREIARIALEKLIQDGRIQPAKIEEK
                     IAYAEQEVKNRIKKAGEDAVYEAGLLDLPSELITLLGRLNYRTSYSQNVLTHSLEVAS
                     LASMLASELGANVEIAKKAGLLHDIGKAVDYEIEGSHVDIGIRILQKYGISNDIITAM
                     KSHHEDYPVETLEAAIIAACDAISASRPGARKENLENYLRRLENLERIATSFPEIEKA
                     YAIQAGRELRVFVFPDKVDDYGTMKLAKTLAQKIEEEVKYPGEIKITMIRETKIIEIA
                     R"
     gene            11970..13022
                     /locus_tag="P5241_00925"
                     /pseudo
     CDS             11970..13022
                     /locus_tag="P5241_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
     gene            13032..13271
                     /locus_tag="P5241_00930"
     CDS             13032..13271
                     /locus_tag="P5241_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV32141.1"
                     /translation="MTFDALNIATKLREQNLQTELFVGDEKIGEQLKYAVNSGCKYCV
                     IYGPDEKNKKEIIIKNLETKEQQNIPLLELTNFHF"
     gene            13324..13647
                     /locus_tag="P5241_00935"
     CDS             13324..13647
                     /locus_tag="P5241_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32142.1"
                     /translation="MVKKLLYKKIGNKTIRCLACHHNCVIPQNKTGICGVRKNTDGEL
                     DLLVYGKSPCYNIDPVEKKPLFHFLPGSQVFSFGTFGCNFGCLFCQNYDISQYSKENT
                     ENIKY"
     gene            13669..14340
                     /locus_tag="P5241_00940"
                     /pseudo
     CDS             13669..14340
                     /locus_tag="P5241_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            14351..15040
                     /gene="amrB"
                     /locus_tag="P5241_00945"
                     /pseudo
     CDS             14351..15040
                     /gene="amrB"
                     /locus_tag="P5241_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
     gene            15140..15577
                     /gene="amrA"
                     /locus_tag="P5241_00950"
     CDS             15140..15577
                     /gene="amrA"
                     /locus_tag="P5241_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRV32143.1"
                     /translation="MLSQIDQIFLLQLSRHTLEYFFETHNKLSMDTISIQSKILLEKR
                     GVFVTLTKKGDLRGCIGNLEPIQTIVESVIDNTLAAAFYDFRFAPLDRSELKDIKIEI
                     SILDSPQKLNIQKPQEIVKFLEQNRPGVILENRYYKATFLPQV"
BASE COUNT         5845 a         1898 c         2594 g         5359 t
ORIGIN      
        1 ttatgtgtac taaggatact ctttctactt ttgattatat tagtgtgaat gtaaatagta
       61 atataacaac ccccccatca tctactacta catcttctgc aacgtcgtct actacttcta
      121 gtacaactac tacaacaaaa ttattagaaa gtggtttttg tggtgtttca acatataatt
      181 tttgtactag acactcagat tgtattatta gtggttgttc tgggcaagtt tgtcaatcta
      241 aaacagaagc tgaactccct acgacttgcg aatgaaagga ttgttattcc aaaataaaat
      301 ataacgtttc atgccaatgt gtaaataata aatgtcaatg aaacaaaaat ataacaacta
      361 gttccagtag tactactacc acaacaactt taccaaaata tcaaaaaatt aataaagttg
      421 atttttattg tgatatagta ccaagatttg ttttccctaa tgagactatt ttatattctg
      481 gtatttttcc taaaaaagat tttggagaat atacgggatt atctaaggtg gatattaagt
      541 gattttacgc gccaaacagt aatgatgaat ttatagctat tgatactcct gagacattac
      601 aaattaattt aacaaatact tttcttggta ttaaatttga gaaagaagga atatataaag
      661 ctaaatattc tattgtaaca gaatataaag gttattatgg tgaaggcgaa tgtttaccaa
      721 ttgtggtgtc tgaacagcct gcgcataaga ctatgtttat tacttccaaa gaatattctg
      781 gtaatttggg aggattaaaa ggagcagatg atatatgtca atatcatgct aagttaggta
      841 aactttcggg tacatataag gccatgcttt ctgatgcgag ttattctgcc aaagcaagat
      901 tgacacttgc tgatatacca tatagaaatg taaaaggtga attgatttat gcatcaggtt
      961 tagagatttt tgatgagaaa cataagatcg gacgtgtggc atctgatgaa tttggagggc
     1021 ctgcttatga ttacactggt agaggaagta atagtttttg atctaatact tatactgatg
     1081 gaagtattgt tttttctgaa gcgcaaaaaa cttgtaataa ttggacatct acacaagctg
     1141 agggagttac gggattagtt gcaaaaattg attatactgg acctataaaa cccgcggctc
     1201 ctgtaggcgt ctgtggaatt ccccagcatt tgtgatgcat agaacaataa tgttagattt
     1261 atgtaattaa caaaaattat tacaattaag ttaatataat atttagtaat attactacta
     1321 aactactaaa ctactaacat tgcgttagta gtttttttat ttttttatgt tgtatataat
     1381 gtgttataaa atattttaga aaaaatatgt caaatataaa tatatatatt atattcagat
     1441 gtataaatat atcgattata aagaatagta aaatttattt taatattttg tgtttaaata
     1501 tatggcctgt ggaaaactta caggggtttg acccctgtaa gttttccaca ataatacaaa
     1561 aaactatcaa aaaactattg acaaagaatt tgttatttgt taattttttc atatatgaaa
     1621 tatatattta aaaatatttt ttaatattta taattttgtg tggagattaa aaatattttt
     1681 ttataatctt cggtggagat taaaaatatt ttttataatc ttcggtggag attaaaaata
     1741 tttatatttg tatattgttt tgtttttgta tatttatgat attataatac aaatttgaag
     1801 cacataaatt attaatataa taaccaatat acacattata acatagatat aaaacatacc
     1861 aataagtaaa taataactaa taagagtcga gcataattaa gatatagcca gaaacagata
     1921 aattataaca tagatataaa taaaaaatat atcttgctaa gcgttttttt atactattat
     1981 tgaattaaaa ttaggttttg aatattatta tgatgtattt agttctttat ataattaaat
     2041 tgtttaaaat ttattatgcc aacaataagg ccagttttta aaaatggaga aaaatatcat
     2101 attataagca gatcaataga tgagagatgt atttttaaag aaacaaaaga ttatcaaaga
     2161 tttgctcgaa atttatattg atttaatgac agagagacaa ctttacaagc atatagtaga
     2221 ttttatgcta ctgatagcgc atgtaaagaa atattattgc gagctaaata tgacagagaa
     2281 attcttgtca aaattatatc tgtatgtctt atgcccaatc atattcattt attattggag
     2341 caggtaacaa atgatggtat aagccaattt atgaaaaaat tagcaggcgg ttacgcttgc
     2401 tattttaata ataaatataa aagatgtggt cacgtatttc agggcaggtt taaagttcaa
     2461 catatacaaa ctctggacac attattcaat acagcttgtt atattcatac acatttggct
     2521 atatctatga ttagaaaaca aaaaaattat aaaaaaatag atcaaaagaa aataattcaa
     2581 gttatagagt attataaatg atctagttat aaagattgtg ttggtgatcg tgattttcct
     2641 tatcttttgt atttatatcc tttatgagaa attatggcgc aagataaatg acgagaagtt
     2701 acttataaat ggattaaaac aaatataaat ctgattataa aagagacaaa taataatata
     2761 atgtttgaat aaaatattaa aagtaagata taaaatcaag caatataatg caatataatg
     2821 tacgacaatg cttgttagat tattttagca tattttaaat gtgtggtcgc cacatttttt
     2881 aataaatact ttaaaaaaat aacatggaaa atataaattc tcaacatgac aatgtaaaac
     2941 catttaagcg tttcgccaaa tggtttcttt taataataat agcaataatt attatttata
     3001 ttgttattat ttttgttagc cctcaaaatc gtgagtggct ttcaatgctt attggccaaa
     3061 agttagacca acgatctttg gataattata aaaaacaaat tcaatcggat aaatatggtg
     3121 gcacgacacc attagaaaca tataaaatgt ttgttgaggc gttaaaacaa caagatattg
     3181 acttggcagt taaatatttt gtccagggta agcagggaga atataaaaag ttttttgaag
     3241 caataaaaca agagggtaaa tgagatttaa tgatggccga tgtgtccacg agtaaaaata
     3301 atacaagagg atcgcaacaa ggacctaatg cttaccaatt agatattgta gactctaata
     3361 actttttagt tactacagtg tatatagtta taccctgtga tattaatggt cagccgattt
     3421 cggatatctg gaaaatagag agtttttaat ttatagatat ataatataaa ttaaagctta
     3481 tagtgaaaaa gtctataatt cacaaaaaga cttgaaaata aatcatttaa acttatacta
     3541 tgaagaaata ttgaatgttt tttctagtgg tgttgttatt ttgttgtttt tgtaataatg
     3601 ttttggcata tgatatgaat acaacccacc cagagttaac taagttaatt attgataaat
     3661 ataatacaca aacgtctaaa tataaaattg atgaagggtt taaagcctgg atagttaaag
     3721 gaagtattga tgaggattta ggtactcgtt cgttaaatca tttttttgat ccaatttata
     3781 atagaacttg aacgctttca ggaatacaat atattattcc aaatgtcaca tcaaaagaat
     3841 gatcacaaaa tagtgttcga caagctaatt caaacttggc atttttggcc tctctggctt
     3901 attctaataa atcagtattg gaatcagatg ccactcaaac ttatcaaaga gccatttatg
     3961 aatatataaa aggtaataaa gcacaagcat tttatgcctt aggtcatata ttgcatttga
     4021 tagaagattt gactgttcca gcacatacac gagctgagcc tcattctaat ttgattgtta
     4081 gtacttatga aaatagcgca gctcaattaa ctgcttctga ttatacgcaa atatataata
     4141 aaataaatta tgataatctg gctaaacgcg attctttaaa tgaatatttt gatagtgtcg
     4201 ctgtttatac taatacgcat ttttttagtg atggtactat tttagattat gctacgtatg
     4261 cacgcccaga agttatatat caagatataa tagaaacaaa tatagagggt atcaaaaaat
     4321 attatttaga agcacaggat ccagagacac aagaaatata tcgtttggca gtaaggggca
     4381 atctttcttg agcaccccat aataatacca gtccattatc tccgtataat ttaaaagatt
     4441 cattagtttt tcgtgattct tgaaatcgtc tatctaaaaa agcagtatta gaaggtgcag
     4501 gtgtaataga tttgtttttt agagatgtag aaaaatataa aaacgatccg aattttataa
     4561 aacaaatgga aactaaattt ggtataggat atgttatgaa agtattaggg gatgcagttg
     4621 atttaattaa acaaatgttt attggtcagc ctgatgttat tttaaattct agtactactg
     4681 tgcttttgga cacacaaggg gtagttacaa ctattactac acaaaataat tctactgcta
     4741 cacaatcgtc gactataact ccaaatatcc cgactgctac taataaaacc actgcgacga
     4801 ctaccaaggc aacgactact acgaaaaaag ttactaccac tactaaaaaa actactacaa
     4861 ccactgttaa aaaagtaacc acaacaacag ctcagccgat tgtgaggtgt agttttgttt
     4921 atggtggtaa tagtataaat acttctatga tttttaatga aattgcttga atgggcacaa
     4981 aaacaaatgc gagtgatgaa tgaattgagt taaaaaatat ttctggtaac agaataaata
     5041 tttctaattg gcaaattgta gataaaaatg gcaacattaa aattgttatt ccgaataata
     5101 cttggattga ttctcaagca ttttatttgc tagagcgttc tgatgatgcg acaattccat
     5161 atttatctgc gaatttaata tatactggtg cattggttaa taataatgaa gagctgcaat
     5221 tatttgatag taattgtaat ttaatagaca gggtagcagc gaatcctgat tgacctgctg
     5281 gcaataatga tttaaaattg acaatggaga ggggaaataa tttgtcttga catacattct
     5341 ccgggaattg tgttaataat atttgtggaa caccttatgc agaaaatagt gttggtgttg
     5401 atgctacttt tagtgataca aacagtaatg gcggaggaac atataataat agtaacaata
     5461 ctacaacaac gagcactact attagttcaa ctactaccac tactaataat tttgatactg
     5521 atactactac ttctactaca tcttctacaa caacttctac cacaactaaa cccgtggata
     5581 tacccataga gcctattgtg tttagtgaag ttcaaataag tggagaaaca gctgatgatg
     5641 aatttattga attatataat cctttggatc atgatataga tttgacaggt tatagtatta
     5701 aaaaattaac tgataataat cttgaaaaga aaacaaattt agtatcagat gataatatta
     5761 gcgggaaaat aattcaatct catggatatt ttcttatagt tcatgccaaa gaaggaagta
     5821 ttaatcaata taaaggtaca acatcacctg atttatattt taatagtagc agttacagta
     5881 tagtacctaa taatacttta attttatatg attctttaaa ccgagaaata gatagaatag
     5941 gttggacaaa taatgaagca gttgatccta tatgtgaggg agattgttta ctaaatatta
     6001 atgatttcca aagttttgaa cgaaaagcca aaagtagttc tacaaaagat ttaatgcaag
     6061 atggtttaga taaaacgttt ggtaattcat ttgactctaa taacaatata caggatttta
     6121 ttataaggga tatatcagaa ccacagaatt cgtttagtat aaaagaacct tatacacccg
     6181 aaaatatatc agaattgata ataccaacac ccactggttt tacagcagtt ttaaacccac
     6241 aagataaaac tatggatttt acctgagata aattagaaaa cgaagataat atagaatata
     6301 gagtatgtta ttcagatcac cctaatggtt gtgatagata tacaacatat actactaatg
     6361 cgtatatgtt tgatgatata ctgcaaaaat ataaaatgaa attggatata tcttcattta
     6421 cgggtgagta ttgatatttt agaatttttg cgcatgatat tactcagaac aaacaagatt
     6481 ctaatttgag tgatgattta gtggtatcta ttataggccc ttattttatt aataattatt
     6541 ttgatgtaat agaatataca aatgatgaaa ataattatat tattttaaaa ggtagggcaa
     6601 aaaggaattt tagcacccaa acttattgtt gacaaggaca atataattgt cattatgggg
     6661 atataggttg agcagaattt tttgccggta ctatttataa tcaaaacaaa gaatttataa
     6721 aatatactgc gtttttatgt aaaacacctc aagaaatact ttctaatggt ttaaatgcag
     6781 tatgcaatta tgaacaaggt aaggattatt cattatatct tactaaatat tcgaattttg
     6841 atttagatac aaacaatagt gctttattgc aaacgtcaga ttttgatcaa gcaaatcaaa
     6901 ataattttat tattttgaga actactgtta ctggtaatat tccagctttt gatccacaaa
     6961 ggcattatta tacttataaa gaatttatta acgaagatat tacaactact acaagttcta
     7021 caaccagtac tacaataccc gctatttctt ctactaccac aacaactgat actactcagc
     7081 ctccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgatac tacaacctca
     7201 acatctacgt cgtcgacatc tactacaact tcttctactg ccacaacaac tgatactact
     7261 cagcctcctg atactacaac ctcaacatct acgtcgtcga catctactac aacttcttct
     7321 actaccacaa caacttgtac tactcagcct cctgatacta caacctcaac ctctacaaca
     7381 acatctacca caactaccac agcacctaaa gtttatacaa atcctatggt agatatttta
     7441 gatttttata cagatttgga aggatatgtt agaatacgag taagattttt ggaaaatgta
     7501 attatgaagt gacaatatgg agaccaggca cattgttatg gttatggtga accaagaact
     7561 tgattttatc attttagaat ttgacaaact agttgaaatt ctcaaaattt aggattatat
     7621 ccaactaaag attgaactga ttgaacagag acttgaaacc atgttcaaaa ctttatgcaa
     7681 aatcaagtag ttgattttta tgtttcaagt aatacgtgcg gttatgcttt aatgaaagat
     7741 aatttgacta ctgatgattg ggtgaatttt tcaggagtac aaggatcata tggttctgta
     7801 tgagatgacg caaatcatta tatgtggtct gggcaaaaat cttattagat gttatattta
     7861 atttggagca aaaacgttgt taaggttgga taagaattat agggatatac taattttttg
     7921 aataaaaatt ctgttttttg gtttttactg gcatattcgg gtatttagta ttcgaatatg
     7981 ccagtttgtt tgaatagttt tagtgcggat tgaatattat agtgataaaa tgacttatgt
     8041 aagtattgtt tttatttttt tgttataata aggtattatt atattaccac aacaaaacac
     8101 atacaaaggg ttgatatttt tttaagaata tgttaatata tagtcattaa ttaaaagttt
     8161 aaaattatat tatggcgaaa attaaaggat tattacgagg gtttattagc agtatgggta
     8221 atatatttca taaaacatga tttaaaatta catttcgtgt tttagttgtt ttgtttatag
     8281 ttagtgcaat tgtaacacag gggtttactt atatacctcg tttgggaggt gttaaaaatt
     8341 atcctgctac aaaaaaagca atggaatttt taagttcaca aatagaccag gcaaaacaaa
     8401 tgggacagct tgatcctact gtacaaatag aattagctaa tgccaaaatt caaaatggag
     8461 taattgtttt gactgttaaa atcggaggac aagatttaga tgcgtatatt accaaagatg
     8521 gttctttgtt ttttcctcaa gcatttaatc taaatcccca agttgaaaaa tttagcgtca
     8581 aaaagactga tgtggtagat gttaaattat ttactatgtc atattgtcca tttggtaatg
     8641 aggcagaaac tgcaataatt cctgtagcta aattgttgaa tggctatgta gatattattc
     8701 cccattatgt tatatattct caatatccca gtcctgaatt atatagtgag tattgtttag
     8761 ataaagaagc aaagtattgc tctatgcatg gtatacaaga attaaaacaa gatgttcgag
     8821 aattgtgtat ctgaaaatat cagaaaaatc tttattgaga ttttatttca caagttaata
     8881 gtatgtgtac tgttgataat ataaatacat gttgaaaacc gattgctact aatttgaaga
     8941 tagataccaa taaaatagaa acatgcgaaa aagaagaagc atctgttttg ttggacactg
     9001 aagtacaatt aaataataca ttacaagtag ctggttctcc gatgttattt attaatgaaa
     9061 cagaatatac aggatctaga actcctgaag catataaaaa tgctatttgc gatgctttta
     9121 ctaaatctaa gagacccaaa gtttgcgata ctaaattaag tgcagatgta ggacaagtat
     9181 caggtagttg taaataaatt ctgtcccaaa gacgtggatt tacgtagatt tagataatat
     9241 gctgataata tctagataga ataaagatac tgatacacgt ggataaaatt agaagtatca
     9301 aagtttaaga gagtataaat acttagcaac taaaaaagtt taacagttta attatttatt
     9361 taaaagtata aatgctaata catattagtt aaggcaaagt cagatagaca aattcggatt
     9421 gtatttttta aaattacata attaagaatt gttatttatt aaatgttcaa agctatgttg
     9481 tttatatagc ttagtagaag agtttgtttt gtatcaacat agcaacaccc acattgactt
     9541 tgccatatgg gcatgtagct cagtggaaga gcgcatctct gataaagatg aggccgaagg
     9601 tccgatccct tccatgccca cttataaagt cgaagttggg cgtggtaatg acaaggtttg
     9661 tagtaaaaat agttattaaa atcttctatt accaataaat ataactgtta tatttttagt
     9721 ttcaacgcgt attgatagta ttgaatattt gtttttgtat tttgatataa atttataata
     9781 atgggcgggc ttatagttta atggtagaat acctcatttg caatgaggag atggcagttc
     9841 gattctgcct aggtccacta atttagacat tagcactgaa aaattataag ctgatttctg
     9901 acgttaattc tcgcaaacta aaatacttat tatttaatat atcttttact aattgcttta
     9961 cttatatttt atgagaatat cttagcgaat gagtgttgga gttggcaaga taatagtagt
    10021 gttcaatttg atattgaata taataagcga aattttttaa atctaatagt ttattgttat
    10081 atcgtatgtt tttggtgtat tgcgtttttt taagaaatat gctaatatat aaaagtaaac
    10141 acaaaggcta ttttttaata atcaagaaaa agaacatcta ttttaattgg ttttctatta
    10201 tttagtttga attatataat atttagaata ttataaacgt tttaggaact taaagattat
    10261 tatttatgaa tttaatattt attgattaca tgctaaatta ttttaaatct actcgctggt
    10321 ctattatttg aaaatagatt gttctttttg tatttaaatt actaacatgc aaaatataca
    10381 tttttttata atcggtgcca gttgtttgtt gcttggtgtt attctggggt atttaattag
    10441 acattttatt ggagtattaa aatctggatc tacagaagct aaattacaac agaaaattca
    10501 aagtactaaa gaagaggcaa aaattattat tttaaaggcc aaagaagaag cagccaaaat
    10561 tattaccaat gcacaggcag aagagaaaga aagaaaaagt caattggatc aattggagag
    10621 tcgtttatta aaacgagagg aactttttga tcaaaaacaa aatgaattag aacaagaacg
    10681 taagaaatta atgggagata tcgaaaaggt taaaattatt aaggaagaat tggcacacgc
    10741 aaaagaaata gaagataaaa gattggaaca aatcgctaat atgagtaagg aagacgctaa
    10801 aagtatttta gtaaaacatt atgaggatga gcataaggag gaattgttaa agatgctaca
    10861 agatttcgaa caagtaaaaa aagaagaagt tgaaaaacgg gttgaagata taattattaa
    10921 tacaatacaa agatatggtc acggcaatgc ctctgagact atgatttcta ctgttgctat
    10981 tccttcagaa gatatgaaag ggagaattat tggtaaagaa ggtcgtaata tacaccattt
    11041 tgaaaaagtg tctggtgtgg aagtgattat tgatgattcc ccagacatta ttactttaag
    11101 ttcttttaat ccaattcgtc gagaaatagc caggattgcg ttggaaaaac ttattcaaga
    11161 tgggagaatt caacctgcta aaattgaaga aaaaattgct tatgctgaac aggaagtaaa
    11221 aaatagaatt aaaaaagctg gagaagatgc tgtgtatgaa gctggacttt tagatttgcc
    11281 atctgaatta attactttac ttggtagatt aaattatagg acaagctata gccagaatgt
    11341 tttgactcat tctttggaag tagcgagttt agcgagtatg cttgcttcag aattaggagc
    11401 aaatgtagaa atagcaaaaa aagctggatt attgcatgat attggtaaag ctgtagatta
    11461 tgaaattgag gggtcgcatg ttgatattgg tattcgtatt ttacaaaaat acggcatatc
    11521 taatgatatt attacagcta tgaaatccca tcatgaagat tatcctgttg aaactttaga
    11581 agctgctatt attgctgctt gtgatgctat ctcagcttca aggcctggtg cacgaaaaga
    11641 aaatttagaa aattatttac gtcgtttaga aaatttggaa cgaatagcta cttcttttcc
    11701 agaaattgaa aaagcttatg ctattcaagc tggtcgtgaa ttaagggtgt ttgtatttcc
    11761 tgataaagta gatgattatg gtacaatgaa attagctaaa acattggctc aaaaaatcga
    11821 agaggaggtt aaatatccag gtgaaattaa aattactatg attcgtgaaa ctaaaattat
    11881 tgagatagca cgttaatata tttacgtttt ttttgttgta atttgctata ataattggag
    11941 tgtaataaaa aataaaataa atataaaata tgaagacgaa tattataaat acacagccag
    12001 cttcagggtt tagagatttg ttgccaaaag aaaaaatatt taaacaatgg tgtattgata
    12061 ctattagttg tgtttttagt aaatatggct atgactctat agaaactcca agtttagaga
    12121 gattagaaat tttagaaggc aaatcaggag aagaatttaa taaattaata tttaaaattg
    12181 cccaaagagg tgataaagag tctgaagatc cagatttagg attgcgttat gattttacag
    12241 taccattagc aagattttta gcagaatata aaaataaatt acccaaaatt tttaaacgcc
    12301 agcaaatagg tccatgctga cgcgccgaaa gggcagggaa aggtagattt agagaatttt
    12361 atcaggcaga catagatact attggttcaa aatctttatt agtagaagta gatactatat
    12421 tggctttatc tgaagcttta aataaaatcg ggcttaacaa tttaattgta aaaataaact
    12481 cacgagaatt attacaagtg attatggatt tatataaaat tgcacccgag aaacaaaatg
    12541 attgtcttgt tgctttagat aaattttata agattggtaa agatggagtt gtacaagaat
    12601 tggtaagcag gggatttaat aaacaaattg gagaagatat tgttgatatt ttgtcagata
    12661 caactttatt acgaaaaaaa atagaattaa atgaattagg cgcaaaatct ttaaaagata
    12721 ttgagcagat agtaaactta gtaaacaata atactaaata tgctatagca gaatttgccc
    12781 cttttataat tagggggcaa gattattata ctggtattat ttttgagatt tattcgggag
    12841 atatacccgg gtcaatggct ggaggtggca gatttgataa tttaattagt atatttagtg
    12901 atgataataa tataccagct tgtggaggat caattggtgt ggagagagtg gtgtctgtat
    12961 tagaagaatt gggtataaat agtattacga atatacaacc aaaaatttta gttagtgtat
    13021 gagataatac tatgacattt gatgctttaa atatagctac taaattacga gagcaaaatt
    13081 tacagacaga attgtttgtt ggtgacgaaa aaataggtga acaattaaaa tatgctgtaa
    13141 atagtggatg taaatattgt gttatttatg gcccagatga aaaaaataaa aaagaaatta
    13201 ttataaaaaa tttagaaacc aaagaacagc aaaatattcc attactagaa ctaactaatt
    13261 ttcattttta ggtgtagcaa ggatagctaa agtatatttt atttaatcta taatgtatat
    13321 attatggtca aaaagctttt gtataagaaa attggtaata aaactataag atgccttgct
    13381 tgtcatcata attgtgtaat tcctcaaaat aaaacaggta tttgtggagt tagaaaaaat
    13441 acagatggag agttggattt attagtttat ggtaaaagtc cttgttataa tattgatcct
    13501 gtggagaaaa agcctttgtt tcattttttg ccgggaagtc aggtattttc ttttggcact
    13561 tttggatgta attttggttg tttattttgt caaaattatg atatttctca gtattccaag
    13621 gaaaatacag aaaatatcaa gtattgaggg gaagatttat ctcctcaaca aattattgat
    13681 ttttgtctat ctaataatat aaaagttata gcttatacat acaatgagcc aacaatattt
    13741 gtagaatatg ctttagatgt tatgaaattg gcaaaaaaga ataatataaa aaatattttt
    13801 gtttccaatg gatttatgtc tgacgaaact attgaattaa taaaaaatta tttggatgct
    13861 atcaatatag atttaaaaag tttttctgat gatttttata gaaaaatttg ttttgctaat
    13921 attgaaccag taaaaacaaa tataaaaaca ttttatgatt tgcacatttg gcaagagatt
    13981 actacgttaa taatatctgg actaaatgat tcggatgtcg agttaaaaaa catagcaagt
    14041 tttatttata atattagctc agatattcct tggcatatta gtgcttttta tccagcttat
    14101 aaaatgattg atagattatc aacatcgatt gatatattgc aaaaagcata tactattggt
    14161 aaaaaagttg gattaaaata tatttatatc ggcaatgtca gcaataatta cgaggacact
    14221 atttgtccta attgtaaaaa tattttagtc agaagacaag gtttttttgt taaagaaaac
    14281 agtattgtta ataataaatg tccaaaatgc cagacattaa ttgccggcat ttggaattaa
    14341 catttacaat atgcttaagc gtttagctca atttactggg agtttttatc ctcaaaatcc
    14401 agttgaatta aaaaacctaa ttcaaaaatt ttttaatata gcaacagtat ctaaaacaaa
    14461 aacaaaacca aaggcattat tggtaccaca cgcagggtat atttattctg gattatgtgc
    14521 tgcttatggg tataaaacaa ttgaaaaata taactataaa aatattattc ttatagggcc
    14581 tagtcatgtt acttattttg aggggttaat tagcacaccc agatgtattt gagagacacc
    14641 tttgggtgaa ataaatgttt atagtataaa tgaattattg cccaaaaagg aatgtattca
    14701 ggagtcgttt gatatatttg tgccagaaca tagcttagaa gtgcagatac catttttaca
    14761 atatatttat aaagatttta ttgtttttcc tatattagtt ggtaatatag attttttgaa
    14821 tatttttgat atattagcaa aattagattt atctgatagt ttaattattg ttagttctga
    14881 tttgagtcat tatttaccat taaaagaaaa ttcaatgcaa gataaaatta gtttagatgc
    14941 cataaaagat ttggatagcg agagatttga taaattcgga gatgcttgta acaaaaattc
    15001 cattagtgct ttattaaaat tagctgatta ttataaatga cagtcattaa ttttatatca
    15061 aagtaatagc tccaatgtaa ccaacgatac aaattctgtt gttggatatg cgagtgtaat
    15121 attttattaa atttatatta tgttatctca aatagatcaa atatttcttt tacaattaag
    15181 tcggcatact ttggaatatt tttttgaaac gcataataaa ttaagtatgg atacaattag
    15241 tatccagtct aagattttat tagaaaaaag aggagtgttt gttactttga ctaaaaaagg
    15301 agatttacgt gggtgtattg gtaatttaga acctattcaa acaattgttg aaagtgtaat
    15361 tgataataca ttagccgcag ctttttatga ttttcgattt gcacctttag acagatcaga
    15421 gcttaaagat attaagatag aaatatccat tttggattcc ccacaaaaat taaatataca
    15481 aaaacctcag gaaatagtta agtttttaga acaaaatagg cctggagtta ttttagaaaa
    15541 tcggtattat aaagctactt ttttaccaca agtttgagaa gaaataaatt cggcccaaga
    15601 gtttttggaa tctctgtcta ttaaagctgg tttaaataaa gatgcttgaa aagatatgaa
    15661 cactacgctt ttaatttata atgtccaggc atttaaagaa aaatagattt attgtattaa
    15721 cattttttag attttagacc ccttgacata atatgtataa tgtgctatga ttatattgta
    15781 tcatataaat tatgat
//
LOCUS       DAOTJA010000007        14337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_4655_length_14337_cov_6.047892, whole genome shotgun sequence.
ACCESSION   DAOTJA010000007 DAOTJA010000000
VERSION     DAOTJA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_14337_cov_6.047892"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1383)
                     /locus_tag="P5241_00955"
                     /pseudo
     CDS             complement(325..1383)
                     /locus_tag="P5241_00955"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1918..2115)
                     /locus_tag="P5241_00960"
     CDS             complement(1918..2115)
                     /locus_tag="P5241_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32144.1"
                     /translation="MKLPFSKKSTKTTPIIPSEYAQAVSTLRDLIAPSGLVVNSTSIK
                     LGETYCRTLFILTYPRYLSMN"
     gene            complement(2125..2799)
                     /locus_tag="P5241_00965"
     CDS             complement(2125..2799)
                     /locus_tag="P5241_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32145.1"
                     /translation="MPKTLNVQETFGSQSLLPISEIRNNIIILKNGALRSLIEIKGVN
                     LMLMPQEEQESIIYSWQSFLNNLEFSLEVIALSRKTNIDQYISQITEATKTEQNQLLK
                     YQIDDYITFISDFIKNYPIMKKTFYIVVPYDPIVIKTSTIFSNIKDMFSNILNLKREA
                     FATETIMPEEEFQRNYQQLLIRQDTILTYLIRMGLEANIVQTDEIIQLFYNLYNPDNA
                     SSKIIV"
     gene            complement(2971..3219)
                     /locus_tag="P5241_00970"
     CDS             complement(2971..3219)
                     /locus_tag="P5241_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRV32146.1"
                     /translation="MQSFQVPQYIEEESRLIGSLTFTQVIILVFGGALVYLSYSLLTS
                     WVSLLLIILIGSATIILAFLQVDGTPTYKLIMPLIKHI"
     gene            complement(3254..4396)
                     /locus_tag="P5241_00975"
     CDS             complement(3254..4396)
                     /locus_tag="P5241_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32147.1"
                     /translation="MQNNINIDYLLQNMPEDIYQAMLDYDINNQIKSLIIALGLSETA
                     SSIFFKEISNVLMGIKTIDDFKNTLITQLNLNETLANFAITQLNTIVFNSLENSILYM
                     QNIFKKQTANATNNITNIETDKQNINNIQTPSTTQSYEQNMEQILNVIKNLSQKQVSN
                     PSAIISEPTIVKEANPDISSNKNTKIDTANINTAMPQTSMIPTSTQQSVPINNPNEIL
                     KDRPPTNKKDKPNKLLEAMEQMQTPKTKIENLIQSYHFNTNTNLPNNNTQEQQSQDIE
                     FESPFKIKKTPSIIQKGESETNNTTHPIDINPADVPDILKMKKMREPIAPSIKNIDLE
                     SPVNNPNISQTPIKYKKITTQPTNPYQYEQGSKNTAQTKFIDLSNT"
     gene            complement(4405..4992)
                     /locus_tag="P5241_00980"
     CDS             complement(4405..4992)
                     /locus_tag="P5241_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32148.1"
                     /translation="MKEMKIFMRPNKEPLTNFAKYVNKIESKVGNFDDFIKKQLKSNK
                     LTEEQKNTLNTATNIAEISKLLAMSQPQTITDDDKLNTYINKLQNAIKEVLKNTTVPL
                     LPEIINKLQNINVPSKQSNMFLLVMANQVLPQAKENELHNFFNNIPDDQKHSLYQQLN
                     TEYSKINKTSLTETQGFKDLKYHNPILYNKIIGKT"
     gene            complement(5203..5520)
                     /locus_tag="P5241_00985"
     CDS             complement(5203..5520)
                     /locus_tag="P5241_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32149.1"
                     /translation="MKEFGLDVNKIKAVNLDALYKFLSTNVDENGKPLIDSLITFYQS
                     ATSNAKTTSALFSKTKPQYMNEFIEKRNKELAPLGKTFFGIMGEEGYKKITHESMAGQ
                     LFE"
     gene            complement(5590..5904)
                     /locus_tag="P5241_00990"
     CDS             complement(5590..5904)
                     /locus_tag="P5241_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32150.1"
                     /translation="MGQRHKEAIKKDLEAGEKEISKIDKKNAAIMAQTAKRLFESPSP
                     LTNDKTKAFQVMTDIMNGIQKYDYELDDILKIYPNYLKDIASLSQDPRYANLDVVDDI
                     KK"
     gene            complement(6013..6276)
                     /locus_tag="P5241_00995"
     CDS             complement(6013..6276)
                     /locus_tag="P5241_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32151.1"
                     /translation="MLNLPLLCAALFFILRLIYLISDQANEAMKSAGGSLSMSTVIGY
                     TMMIIILAQSMLIVASALNVNNISKITKKTREIIQGGVGAGLK"
     gene            complement(6373..6894)
                     /locus_tag="P5241_01000"
     CDS             complement(6373..6894)
                     /locus_tag="P5241_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32152.1"
                     /translation="MMRDIANMMIMIMIFIIAMGIMLGNKEFQNYQALIYLVIVALLI
                     NFSLVICGMLIDISNYFTVFFLHNGGIENLECTLVMTIRRVSNIFQIGQPKWWQKLII
                     LFTKTSPAHFILPLMPVAHFMDKMIYKVAGIEEAFPMAMTVSSILGLIVATILCGQII
                     GLCIYVLTRIITL"
     gene            complement(6958..7107)
                     /locus_tag="P5241_01005"
     CDS             complement(6958..7107)
                     /locus_tag="P5241_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32153.1"
                     /translation="MNHPADALLAGIPKLLTSLLTFVIKIMARVLQLFIMPNNSVTVL
                     TFIGR"
     gene            complement(7114..7293)
                     /locus_tag="P5241_01010"
     CDS             complement(7114..7293)
                     /locus_tag="P5241_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32154.1"
                     /translation="MHFGIMSKKCKNVIIILLIGTILLPYFSVTNIAKADNIPSDDSG
                     EFDRCPVEAVQTFTF"
     gene            complement(7489..7797)
                     /locus_tag="P5241_01015"
     CDS             complement(7489..7797)
                     /locus_tag="P5241_01015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV32155.1"
                     /translation="MLHNNNAVDIANSCGTPIYAAADGIVISISSGGGWNGGYGNSIK
                     IQHPNGTTTLYAHLSSINITKGASVKQGDFIGKMGSTGKATGCHLHFEVNGAKNPFVK
                     "
     gene            complement(7807..8592)
                     /locus_tag="P5241_01020"
     CDS             complement(7807..8592)
                     /locus_tag="P5241_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRV32156.1"
                     /translation="MLISIILLLGLSTILSYSTTLAQNNTILEDQISSNTQAQNINTS
                     DEHNPFTAIGDSELISEFAPIETLNCDNTITDAALSCQDYYSNLINPKTPEDFLNIYL
                     VEDSEFVFGTQNQSQQILQAPSERNQLMTYIVQKGDSLEKIAKKFGISVDTIKLANRL
                     TKTILQPGKELIILPVSGIYYTVQKGDTLSKIANTYKISVSSIIEYNGIDEHNIKIGD
                     KIILPGAKTIAQSTQSNYATNSIPSAINNTNTITGYYIYPTTG"
     gene            8852..9604
                     /gene="rsmA"
                     /locus_tag="P5241_01025"
     CDS             8852..9604
                     /gene="rsmA"
                     /locus_tag="P5241_01025"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRV32157.1"
                     /translation="MKQLKSLGQVFLQDTRYLSDILLAAQISKNDYVLEIGPGQGILT
                     QPLIQAGARVLAIEKDSRLVEYLKTHIQNPNFKVIQGDIRYIYLDIIQKQSNSFKIVA
                     NIPYYLTSFLLRMILENNPKPELCVLLIQQEVAERLIAKNNKESVLSLFVQYYAEVLY
                     IKKVPRQAFNPVPQVDSAIIKIIPKKDIIPNKEFTDKLFKIIKVGFISPRKYVLSNLK
                     NGFTQDKDKFTQIFHDLNISMQARAEDIGLET"
     gene            complement(9681..11372)
                     /locus_tag="P5241_01030"
                     /pseudo
     CDS             complement(9681..11372)
                     /locus_tag="P5241_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            complement(11424..11858)
                     /locus_tag="P5241_01035"
     CDS             complement(11424..11858)
                     /locus_tag="P5241_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32158.1"
                     /translation="MNFKEFSTKINNTDIKIRFSDIAKMANGSLLAQIGDTMVLATVI
                     MGKEDKLDYDFMPLTVDYEEKYYAAGKIYGSRFVRRESRPTENAILTGRLIDRTLRPL
                     FNDKIRRDIQIIVTCLSIDENNDPDVISVLASSLCLLTSNVP"
     gene            complement(12014..12571)
                     /locus_tag="P5241_01040"
                     /pseudo
     CDS             complement(12014..12571)
                     /locus_tag="P5241_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(12571..12831)
                     /gene="rpsO"
                     /locus_tag="P5241_01045"
     CDS             complement(12571..12831)
                     /gene="rpsO"
                     /locus_tag="P5241_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRV32159.1"
                     /translation="MAKLEDKNKIIKKYRIHTTDTGSPEVQIAVLTERIKDLAAHLKK
                     HPQDVDSKRGLLEIIAKRRKILMYLEKKYPKRYNSLMKHIKI"
     gene            complement(13144..13599)
                     /gene="smpB"
                     /locus_tag="P5241_01050"
     CDS             complement(13144..13599)
                     /gene="smpB"
                     /locus_tag="P5241_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRV32160.1"
                     /translation="MKTRSENKIITENKKAFYDYEIIEKLEAGIVLHGYEVAAVRRNR
                     VSLTGSYALLHNNELFLINAHISCYQPKNQPADYNPTQSRKLLLTKKEISYLQRQTKE
                     KHLSIIPLKIYIKNKKIKVELGLARGKKKYDKREVIKKRTIERNIKKHN"
     gene            complement(13648..14049)
                     /locus_tag="P5241_01055"
     CDS             complement(13648..14049)
                     /locus_tag="P5241_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRV32161.1"
                     /translation="MSGLIGIITFISLYILGAKYALLAGLIHGCLEFIPFIGPIIASV
                     LILIMNINIAPITWFGILLTLIIIQYIENIVSPIIRGKILKINPLVIIFAITVGAKLG
                     GILGIFIAIPLSTILVTFFKDLSCLKSPDEP"
BASE COUNT         4395 a         2084 c         2109 g         5749 t
ORIGIN      
        1 tattgattat atttactctt atgcttattt attattattg tgttattgtt atattctcca
       61 tcgtagtatt gattatattt actcttatat ttgttattat ttgattgtta tattctccat
      121 tgtagtatta attatatatt taatcttata cttatttatt attatgttat tgttatattc
      181 cccaccgcag tattgattat atgtattctt gtccttattt gctatttttt attattatat
      241 tttccattac agtattaatt atatttgctc ttatatttat ccttatttat tattttattg
      301 ttatattctc catcatagta tttgttatat tgtattatgt tgattgttcc ctcgtttagt
      361 ggctaataat tgttgtgggt cagaagtaat aatttggtcc tctgtatatg aagggataac
      421 ttgtattact gcatgtttat ttcctgctat aaaaagccct gtgccaacgt ctgcttgtaa
      481 taatattgct ttttctactt ctgttaagtg taaggcatta cctattttat caatagtagc
      541 ggatgattgc ttaaataaca tttttaaaga tgaattagta ataagtggca gtccgtatgg
      601 agaatctaag aaatctccga tatcctgagt aatggttgtt aatcctaaat aatattttct
      661 gcttcgtttg gcaatcccaa atataaaatc agcgctttct ttatttttta gtagatatca
      721 agcttcatca ataattaata gacgtttttt catatctttt ttaatattat ttcaaatata
      781 atttagcaca gtatacattg caataggacg taattcttct tctaaatctc ttatattaaa
      841 taccacaaag cgttcattaa tatttatatt agtttgttga ttcaaaaatc cagcataagt
      901 gcctttggta tacttatata atttagcagc catatctgca cctcctttca tagtttctaa
      961 gacagcttgt aaatcttcta aaataggtat attcgctgat gtcgtagatt caatggaaat
     1021 gtcacgcgca gcataagttt gaacgattgc ttgatctaaa agaacttctt cttgtgctgt
     1081 taattctcca attaaaattt ttaccaagcc tatcaaagtt aaaatatggc ttctaaatac
     1141 ttctggtgca gtttcatctg cgcctataat tggtaaatca aatgggttaa tatgattttc
     1201 tgatcctact gaaattttcc aaaaactacc accaattgac tgcgccaaat attggtattc
     1261 attctctggg tctataataa taacagttga tcctaacatt aatgaacgta aggcttcaag
     1321 tttaacacta tagcttttac cactaccagc agtaccaaat acacacatat tggcattttc
     1381 catattaaat ctatcaaaga taactaaact attattatgt acatttacgc catacaaaat
     1441 gcctttgtta tctgtaagat ccaaagatat aaaaggaaat agagttgaag ctggtcctga
     1501 atttaataat acactaaatc ccaatctatc caaaccaaga ggcaaagaac tctcgtatcc
     1561 ttcgaactgg cgaaaattag ccgatttaat atatattaat ttactttcta aaaggctttg
     1621 gactttggct tctatttcat ttaattctgt ctcagatttt gccagaatag taatatatac
     1681 agccactcta aacatttttt cttgtccttg gacaatagca tctcgtaaat tttcaatatc
     1741 ttgtaaagac gtttctaaaa cagggtttct aggtgcgccc cgttcttgct ctaaagcgat
     1801 ttgagcgcca atatctccta attttttacg gagttgtttt aaaatgagag tattatctac
     1861 tggatgataa gaaatagaaa tattaaataa atgttccatt tcaataatgg gttcaaatca
     1921 attcatactt agatagcgag gataagttaa aataaataaa gttcggcaat atgtctcacc
     1981 taattttata gaagttgaat taacaactaa tccagatggt gcaatcaaat ctcgtaatgt
     2041 cgagactgct tgtgcatatt cgctcggtat aattggtgtt gtttttgtag atttttttga
     2101 aaaaggtaat ttcattttac aagtttaaac aattatttta ctgctcgcat tatctggatt
     2161 gtataaatta taaaaaagct ggattatttc atcggtctgg acaatattag cttctaatcc
     2221 catacgaatt aaataagtta aaatcgtatc ttgcctaata agtaattgtt gatagtttct
     2281 ttggaactct tcttctggca taatagtttc ggtagcaaaa gcttctcgtt ttagatttaa
     2341 tatattagaa aacatatctt tgatattaga aaaaattgtt gaggttttaa tcacaatagg
     2401 atcgtaaggt actactatgt aaaatgtttt tttcataata ggatagtttt taataaaatc
     2461 agaaataaat gttatataat catcaatttg atattttaac aattgatttt gttctgtttt
     2521 ggtagcctca gtaatttggc taatatattg atcgatattg gttttgcgtg atagtgcaat
     2581 aacttccaaa gaaaactcca aattatttaa aaaagattgc cagctgtata ttatactctc
     2641 ctgttcttct tggggcatta acattaaatt tacacctttt atttcaatca aagagcgcaa
     2701 ggcaccgttt tttaatatta taatattatt tctgatttca ctaataggta gtaatgattg
     2761 actaccaaaa gtttcttgta cgtttagtgt ttttggcata attatttatt tatcctcttt
     2821 gttaagtatt tttgttaatt ccgatatatc tactttgctt ttacttttat tagtcagtag
     2881 tggagaagca gtttgattag tttctttggt tgattgttgg ggtattgaag tgtttatata
     2941 tggttttttc caaatatata cacggggtaa tcatatatgt tttattaagg gcattattaa
     3001 tttatatgtt ggtgtaccat cgacttgtaa aaaagctaaa ataattgtag cagaacctat
     3061 taaaataatc aataataatg atacccatga tgttaataaa gaataagata aataaactaa
     3121 agctccacca aatactaaaa taataacttg ggtaaaagtt aatgacccaa ttaatctgga
     3181 ttcctcttct atatattgtg gtacttggaa actttgcata ttataatatt tattgtgtat
     3241 atttattgaa tttttaagta ttagacagat ctataaattt tgtttgagca gtatttttgg
     3301 atccttgctc atattgatat gggtttgttg gttgtgtggt tattttttta tattttatag
     3361 gtgtttgaga aatatttgga ttgttaacag gcgattctaa atctatgttt ttaatactcg
     3421 gtgctattgg ttcacgcatt tttttcattt ttaatatatc aggaacgtct gctgggttga
     3481 tatcaatagg gtgggtggta ttgtttgttt cgctctcgcc tttttgtata atgcttggag
     3541 tttttttaat tttgaaaggt gattcaaatt ctatgtcttg gctttgttgt tcttgtgtat
     3601 tattattggg aagattggta ttggtattaa aatggtaaga ctgtattagg ttttcaattt
     3661 tagtttttgg agtttgcatt tgttccattg cctctaataa tttatttggt ttgtcttttt
     3721 tatttgtagg tggtctatct tttaaaattt cattgggatt atttataggc actgattgct
     3781 gagtagatgt tggtatcata gatgtttggg gcatagctgt attaatattt gctgtatcaa
     3841 tttttgtatt tttgttagag gatatatcag ggtttgcttc ttttacaatt gttggttctg
     3901 atataatcgc cgaagggttg gatacttgtt tttgcgaaag atttttaata acattcaaaa
     3961 tttgttccat attttgttca taagattgtg tggtgcttgg tgtttgaata ttatttatat
     4021 tttgtttatc tgtctcaata tttgtaatat tattagttgc attagctgtt tgttttttaa
     4081 agatattttg catataaaga atgctatttt ctaaactatt aaatacaatt gtatttaatt
     4141 gggtaatagc aaaattagct aatgtttcgt taagattcaa ttgcgttatt aaagtatttt
     4201 tgaaatcgtc aatagttttt atccccatca aaacattaga aatttcctta aaaaatattg
     4261 aacttgctgt ttctgataat ccaagcgcaa taattaaact tttgatttgg ttgttgatat
     4321 cataatctaa cattgcttgg taaatatctt ctggcatatt ttgtaagaga tagtctatgt
     4381 ttatattatt ttgcataata tgtattatgt tttgccaatt attttattat aaagtattgg
     4441 gttgtggtat tttaagtctt taaacccttg tgtctcagtt agtgatgtct tattgatttt
     4501 agaatattca gtatttagtt gttggtatag agaatgtttt tgatcgtcag gaatattatt
     4561 aaagaaatta tgtaactcat tttctttagc ctgtggtaag acctgatttg ccattacaag
     4621 cagaaacata ttactttgtt ttgagggaac attaatattt tgtaatttat ttataatttc
     4681 tggcaacaag ggaactgtgg tattttttag tacttctttg atagcatttt gtaatttatt
     4741 tatgtatgta tttaatttat catcatcagt aatagtttgg ggttgagaca tagctaacaa
     4801 tttagatatt tcagctatat tggttgctgt gtttaatgta tttttttgtt cttcagtcaa
     4861 cttattggat tttagttgct tttttataaa atcgtcaaaa ttaccaactt tggattcaat
     4921 tttatttaca tatttggcaa aattggttaa tggttcttta ttagggcgca taaatatttt
     4981 catttctttc aattcttcta atatttttaa taataattgt gttgctttat aatcagaggc
     5041 atcttcttct ggtataccac cagaagtatc tttgcttccg ggctgttttg tttttatgct
     5101 actaacagca tcatttatta tactatcaaa tttaggacta ttaataatat attgcttgtc
     5161 ttggaatgct tgtttaaaat cttccttatg tttgtctgat aatcattcga acaattgacc
     5221 agccatagat tcatgagtga tttttttata tccttcttcg cccataatac caaaaaaagt
     5281 tttgcccaat ggagccaatt ctttgttacg tttttctata aattcattca tatattgagg
     5341 ttttgtttta ctaaatagtg cggaggtagt tttggcattg cttgtagcac tttgataaaa
     5401 agtaattaag gaatctatta gtggtttacc attctcatct acattagtac ttaagaattt
     5461 atataacgca tcaaggttta cggctttaat tttattaaca tcaagaccaa attctttcat
     5521 tgtgccttca acagatgctc gtgtgtatcc tttgccatca atagtttttt tgaatttaag
     5581 ataaggtaat cattttttaa tatcatctac tacgtccaga tttgcatacc tcggatcctg
     5641 actaagcgat gctatgtctt taagataatt tgggtatatt tttaggatat catctaattc
     5701 ataatcatat ttttgaatac cattcattat atctgtcata acttggaagg cttttgtttt
     5761 atcattagtt agagggcttg gactctcaaa tagacgtttt gctgtttgcg ccataatagc
     5821 agcattcttt ttatcaattt tgcttatttc tttttcgccc gcttctaaat ctttctttat
     5881 tgcttcttta tgtcgttgtc ccatagtaaa acctaattta gccaacgtag gatttttgga
     5941 ttgagctcat tgagttgctg tagcttgata ggcttctgat ttttccactc gttttgtacc
     6001 ttctgttttg actcatttta atccagcgcc cacaccccct tggataattt ctcttgtttt
     6061 cttggtaatt ttactaatat tgtttacatt tagtgctgag gcgactatta acattgattg
     6121 agctaaaata ataatcatca tcgtataacc aattactgta gacatagata aagaacctcc
     6181 agcacttttc attgcttcgt ttgcttgatc tgaaatcaaa tatattaatc ttaaaataaa
     6241 gaatagtgct gcgcaaagta gtggtaaatt taacaataag ccagaaaaat atttccatca
     6301 agcattaaaa acttctcggg tttgaggcaa aattcaacat gccatagcca aaggagatac
     6361 gatatttaat gctcataatg ttattatacg agtcagaaca taaatacata agccaattat
     6421 ttggccacat aaaatagtcg ctacaattaa tcccaatata ctgctcacag tcattgccat
     6481 ggggaaagct tcttctatac cagcaacttt gtagatcatt ttatccataa aatgagcaac
     6541 tggcataagt ggtagtatga aatgtgcagg acttgttttt gtgaataata taatcagttt
     6601 ttgccaccat tttggttgcc ctatttggaa aatatttgat actcggcgaa ttgtcataac
     6661 aagagtacat tctaaatttt ctatgccacc attgtgtaaa aagaaaaccg taaaataatt
     6721 tgatatatca ataagcatgc cacaaattac taaggaaaag tttattagta aagctactat
     6781 tactaaataa ataagtgctt gataattttg gaattctttg tttcctaaca ttatgcccat
     6841 tgctattata aaaatcatta tcataatcat catattggcg atatcacgca tcatttctca
     6901 agattctact acaaattggt tattagtcga atatcctgta tagccactat ttgggcttca
     6961 tcggccaata aaagtaagca cagttacaga attatttggc ataataaata attgtaaaac
     7021 ccgagccata atttttataa caaatgttag caatgaagtt agtaatttag gaatacctgc
     7081 caataaagca tctgctgggt gattcaaggc atctcaaaaa gtaaatgttt gtactgcttc
     7141 tactgggcat ctatcaaact ctccagaatc atcgctcggt atattgtctg cttttgctat
     7201 atttgtaaca gaaaaatatg gcaggagtat tgtccctatt agcagtataa taataacatt
     7261 tttacatttt ttggacatta taccaaagtg catataaaaa ttttaattat tttaattaca
     7321 actaacaatt aagtaatatc tgtgtatatc tgtttgttct ttttatctgt gttaatacac
     7381 gtattattta tgtttagtat agtatataat atttttgtaa aaaagtaaat agctttaagt
     7441 gtttttaggt ttttgtaatc ttaaatattt aatttatggt gtataatatt atttaacaaa
     7501 tgggtttttg gctccattta cttcgaaatg taaatgacaa ccagttgctt tgccagtaga
     7561 acccattttc ccgataaagt ctccttgttt tacacttgcg ccttttgtta tatttatact
     7621 acttagatga gcgtatagag ttgtagtacc atttgggtgt tgtattttaa tagaattgcc
     7681 atatccacca ttccatccgc caccactgct gatacttatg acaataccat cagctgctgc
     7741 gtaaatggga gtaccacagg aattggcaat atctactgca ttattattat gtaacactcc
     7801 tcaatttcat ccagtagttg gatatatata atagccagtt attgtatttg tattgtttat
     7861 ggcgctaggt atagaatttg tcgcataatt actttgggta ctttgtgcta tagtttttgc
     7921 gccaggtaaa ataattttat cacctatttt gatattatgt tcatctatgc cattatattc
     7981 tataatagat gagacagaaa ttttatatgt attggctatt ttgcttaaag tatcaccttt
     8041 ttgaacagta taatatatcc ccgaaactgg taaaataatt aattctttac caggttgtaa
     8101 aattgttttg gtcaatctat tagctaattt tatagtatct acactaatac caaatttttt
     8161 agcaattttt tctaaagaat ctcctttttg aacaatatat gtcattaact gatttctctc
     8221 tgaaggagct tgtagaattt gttgtgattg gttttgagta ccaaacacaa actcgctgtc
     8281 ttctactaaa tagatattta agaaatcttc tggtgttttt gggttaatta aattactgta
     8341 ataatcttgg catgaaagag cagcatcagt aatagtatta tcacaattta aggtttctat
     8401 tggcgcgaac tccgaaatta attctgaatc acctattgcc gtaaatggat tgtgttcatc
     8461 cgaagtattg atattttgtg cttgagtatt agaagatatt tgatcttcta aaattgtatt
     8521 attttgtgcc aaagtagtgc tatacgaaag tatagtagac aaacctaaca aaagtattat
     8581 acttataagc aaagataaat aatgctcttt ttttggagca caataaaaat atctgttcag
     8641 cccaattttt gtagcatatg gcttgaacca attgtacaat gtagtgctgt aagttgttat
     8701 atttttttgt attttttcgt aaaatttgat agtatattta tagcatcaaa aaaagcattt
     8761 atacatatta tgttttattt ggtatattag tcgattaaag gctatataat aattaatata
     8821 attttagcat attttttgtt tttttgctac tatgaaacaa ttaaaatcat taggacaagt
     8881 ctttttacaa gatacaaggt atttatcaga tattttatta gccgctcaaa tttctaagaa
     8941 tgattatgtt ttagaaatcg gcccaggaca aggtattcta acacaacccc ttattcaagc
     9001 tggcgctcgg gtattggcaa tagaaaaaga ctcaagactt gtagaatatt taaaaactca
     9061 tattcagaac ccaaatttta aagttattca aggtgatatt agatatattt atttagatat
     9121 aattcaaaaa caatctaatt catttaaaat tgttgccaat atcccctatt atcttacttc
     9181 atttttatta agaatgattt tagaaaacaa tcctaagccc gagttgtgtg ttttactaat
     9241 tcaacaagaa gttgccgaga gattgatagc caaaaataat aaagaaagtg tcttgtcatt
     9301 atttgtacaa tattatgcag aggtcttgta tattaaaaaa gttccacgtc aagcatttaa
     9361 tcctgttcca caagtagatt ctgctataat taaaataata ccaaaaaaag atataatacc
     9421 caataaagaa tttactgata aattatttaa aattattaaa gtcggattta tatcgccaag
     9481 aaaatatgtt ttgtctaatt taaaaaatgg ttttacacaa gacaaagata aatttacaca
     9541 aatatttcac gatctaaaca tatctatgca agctcgtgcc gaagatattg gccttgaaac
     9601 ctgaaaacaa ataactctcc gtattctaca aaaataataa gtattgtata atagtaaaaa
     9661 tttttatttt gttactataa ttagtagcga ttgggttttt tgtcatgttt gtaatgatct
     9721 ggctttttag agaaaggagc tttggatatt cgtggcgaat tgggatgcgt gatatctcgt
     9781 aatgataaag aaattttgcc attttctaca tttctaactt ttagagttaa ttcgtctcct
     9841 actttgacta tatcttctac tctttttaca tgttctgggc ttaattcaga aatgtgtaat
     9901 aatccagatt gataactacc caaatctata atagcgccga aatcttttat ttctgatact
     9961 gtaccatgta cagtctcgcc aggttcaaaa gtttttacaa tgccttcgac aatcgcctta
    10021 gctttattaa tagattctat attctgccca gtaatataaa cagttccatc atcatcaaag
    10081 tttatttctg tatttgtttc ttcggtaatt ttgtttatca ttttaccgcc actgccaata
    10141 acttctccaa ttcttgttga gtcaatttga aaagtaataa ttttgggtgc atattgtgat
    10201 acttgatcac gaggtttatc aatagcttga tttattttat ctaataaaaa cattagtgct
    10261 tctttggctt gtactaagac ttctttaagt attgtaggag taactccgtc aacttttaca
    10321 tccatttgta aagctgtaat tccatttttt gtacctgtta ttttgaaatc catatcgcca
    10381 taatggtctt ctggtccttg gatatctgtt aagattttgt attcattatt atttatcata
    10441 atgcccatag caataccagc cactggtctt gttatgggga ctccagcagt cattaaagat
    10501 aatgtcgaac cacaagttga ggccattgat gatgagccgt ttgatgataa tacttcgctt
    10561 accactctaa ttgtatatgg gaaaatagat ttatcaggaa taacgggttt taaagctttt
    10621 tctgccaaag ctccatgccc aatttctcgt ctattttgtc cacctttatt tttatttaat
    10681 tctcctactg aatatcctgg aaaattatat tgatggaaat atcgtttttc accagttatt
    10741 tccatacctt gtatagtcaa agtttctttg ggcgctccca aagtagttac tgataatact
    10801 tgcgtatcac ctctaacaaa taaagctgat ccgtgagttc ttggcaaaat atccaaaaca
    10861 atatctatag gtcttatttc attcattttt ctgccatctg gtctgatatt atctgtgagt
    10921 attttattat gaactattga atttgtgatt tcatcaatag ctatatttgc caattttaat
    10981 ttgtcttctc caatatcttt ggctttcaat tcatctataa cagatttatt aagagcgttg
    11041 agtaaaacat ctttttcttg tttggttttg gtattatcaa ataaaatatt ttcaagatct
    11101 tccttaacaa tattttttat ttcatttata aaatcatcgt ctatttcttg taaaacaatt
    11161 tgtgtttttt cttttttata ttcttgagct atgttttctt gaaattcaca tagctgggta
    11221 atctcattct gcccgatttc taatgatttt tgtataatat cttctggtac ttctttcccc
    11281 tcggcttcta tcatatttat tttatttttg gtgccagaaa taattatatc aaaatctaaa
    11341 ctttctcgtt cggaatatgt tggattgatt ttaaaaccat tattgttact tcaaccaata
    11401 cgcacagccg caatcggtcc agatcaaggg acatttgatg ttaataaaca taacgaagat
    11461 gccaagacac ttataacatc tggatcgtta ttttcatcaa ttgataaaca tgtgactatt
    11521 atttggatat ctctacgtat tttatcatta aataaaggtc gtagagtgcg atcaatcaaa
    11581 cgcccagtta gtatagcatt ttctgtgggt cttgactctc ttctaacaaa ccttgagcca
    11641 taaattttac cagctgcata atatttttct tcataatcaa ccgtcagggg cataaaatcg
    11701 tagtctaatt tatcttcttt acccataatt actgtggcta gtaccattgt gtccccaatt
    11761 tgtgccaata aagacccatt ggccattttg gcaatatccg aaaaacgaat ttttatatcc
    11821 gtattattta tttttgtgct aaattcttta aaattcatac ttatagttta aaatagtttt
    11881 taagaccaac tcaaaaacta attagttaat ttattattca gggcaatgta atgacgaaaa
    11941 aatattttta atatcttaat catatttttt cgtctggcat tgctccgtga tattttattt
    12001 attttgtaaa gtattatttt tttgattttc tttttttgta tgtgtttgat ttatttgatt
    12061 ttgatgtaga tatgggtatt agatattgat gtgggtgttc tgaaatatca aaaaaatcat
    12121 cagccaactc tttgagtttg ccattagttg tttgtccaag tccaactatt tctatttgtt
    12181 tacccaatgt ttgtaaatat tgtaccaaag gaataaaatc tccgtcacct gaacacaaaa
    12241 taattgtgtc taacatcggg gacattttta tagcatctac agcaatacct acatctcaat
    12301 cagctttttt agtgccatca ggataaattt gtaaatcttt tacttttata tcaacaccta
    12361 atctttctaa catttcaaaa aaagaacttt cagtctgttc ctctgttttg gcagcataac
    12421 caatagcacg aattaactca cgattatcca aagccgtctc aataacattt agaaaattaa
    12481 ttcttgcatt ataaaaattt tttgccgaat gatacaaatt tggtatatca ataaaaattc
    12541 ctactctttg atgtatatgt ttattagtca ttatatttta atatgtttca ttaacgaatt
    12601 atatcttttt ggatattttt tctctaaata cattagaatt ttccttcttt tggcaattat
    12661 ttccaaaagc ccccttttgg aatcaacatc ttgtggatgt tttttaagat gagctgccaa
    12721 atcctttatt ctttcagtta aaacagctat ttggacctct ggagatccag tatcagtagt
    12781 atggattcgg tattttttga taattttatt tttgtcttct aatttagcca tattgtaaat
    12841 ctgtttattg ttttatatat atttatacta tcatactttt tgaaaatata caatagttag
    12901 gcacaagtat gataaaatct aagtaaaata ggactgctag ataattaaga aatctgagtg
    12961 aaatattaat aaaaattata tctttttatc ttagctaatc tttgtattga tcctcattta
    13021 ttcatatttt atcttggtcc gtgtgtatct gtatttattg tgatcgtatt tgtttgcaag
    13081 ttgtatttat agtttttgtt tttttgctga tgtttaatta tttgttgtta tttttggttt
    13141 ctatcaatta tgttttttga tatttctttc tattgttcgt ttttttatca cttctctttt
    13201 gtcgtatttc tttttacctc ttgccaatcc taattctact tttatttttt tattttttat
    13261 atatattttt aaaggaataa ttgaaagatg tttttctttg gtttgacgtt gtaaataaga
    13321 tatttctttt tttgttaata aaagttttct tgattgtgtg gggttgtaat cagctggttg
    13381 atttttgggt tggtaacaag atatgtgagc attaatcaaa aatagttcat tattgtgtag
    13441 caaagcataa ctgccagtca gagataccct atttcttctt acagcagcca cttcgtagcc
    13501 atgtaaaaca atacctgctt ctaatttttc tataatttca taatcgtaga aagctttttt
    13561 attttctgta attattttat tttctgagcg tgtcttcatt tgtgtatatt atatcaatta
    13621 gttagtttga ttattgtttt tttcacatca aggttcgtct ggtgatttta aacaacttaa
    13681 atctttaaag aatgttacca aaatagttga caaaggaata gctataaata tcccaaggat
    13741 cccacctaat tttgcgccca cagttatagc aaaaataatt accagaggat taattttgag
    13801 tattttccct ctgattatag gagaaacaat attttcgata tattgaataa taattagtgt
    13861 tagtagtata ccaaaccatg taataggtgc tatgtttatg ttcataatta aaattagaac
    13921 cgatgctata attggtccaa taaatggtat aaattctaag catccatgta tcaacccagc
    13981 aagcaaagca tatttggcac ctaaaatata caaacttata aatgttatta tacctatcaa
    14041 accacttata agaatttggc tatataatca aaatgtcatt ttgtttttgc ttttttctca
    14101 taaatctaca atatattttc tatatttttt tggagcaaaa aacccaagta aatttttgaa
    14161 tcaattgttg tctaaagaaa tataaaaaga tattagcagc attaataaaa tatctgtaaa
    14221 ttttgagaaa aatagtccaa tatttgctgt taaatctttt atataggtta taatattttg
    14281 tgtattcaat cactggctcg ttataatatt ttctgaaata aaattgttta taatatt
//
LOCUS       DAOTJA010000008        14328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_4666_length_14328_cov_4.984026, whole genome shotgun sequence.
ACCESSION   DAOTJA010000008 DAOTJA010000000
VERSION     DAOTJA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14328
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4666_length_14328_cov_4.984026"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..535
                     /locus_tag="P5241_01060"
     CDS             191..535
                     /locus_tag="P5241_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRV32162.1"
                     /translation="MQDNKGFTLVELMIVIAILAILAAVVVFALNPAQLMARARDSQR
                     ISDLQALTTAINYFTVISPTPYLDNATNSTCKGIATTATIYATVTSATAPGGAFAYNT
                     TGVTSRAMDGSG"
     gene            complement(813..950)
                     /locus_tag="P5241_01065"
     CDS             complement(813..950)
                     /locus_tag="P5241_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32163.1"
                     /translation="MVIFKDAGRFLIIQYKINPAILIKAMVGLNSLYTKLFQFVNIDI
                     L"
     gene            1441..3147
                     /locus_tag="P5241_01070"
     CDS             1441..3147
                     /locus_tag="P5241_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRV32164.1"
                     /translation="MAISLQVLKPILLQSGLVSEDTFNKALETSKRTGQNVTDILISN
                     GSITADYLTEIISNYLKIPQVRLRSKDIDYDILNIVPEDIARTRNVAIFGKDKEYLLV
                     AMTDPTDLETIEFLQRYTNHPIKVYITTDDDLKSSFALYRQKIAENFKNIIEDNIKQA
                     AKIAGISLEQAAVELPIVALFNNIVEYAASLNATDIHIERLNDAVLIRFRIDGLLKEI
                     IRLPKETHPAIIAHIKILSNLQIDEHNKPQDGRFKYQRGEEVFDIRVSILPTMYGEKA
                     AMRLLLGALKPLSFEELGMQPYSTEIINQNIKRSYGMILVTGPTGSGKTTTLYSILSK
                     LNRPEVNVVTIEDPIEYEMKYINQIQVNEKSGLTFANGLRAIVRQDPNIIMVGEIRDG
                     ETADIAVNAALTGHLVLSTLHTNDATTAIPRLFDMKVEPFLVAATINAILAQRLVRRI
                     CKDCITSIPVDQNIKDLIKKYYLDSPYKKTKAIIPNQLYKGKGCKSCNFTGYKGRLAI
                     YEILNINESIRTLIQSSRFNLDTLREAAGKTNMITMFEDGLIKAELGLTTIEEILRVI
                     KE"
     gene            complement(3544..3738)
                     /locus_tag="P5241_01075"
     CDS             complement(3544..3738)
                     /locus_tag="P5241_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32165.1"
                     /translation="MALVIFSPFFKLILVRENILEGILVNIGVDQTIEFVSILNVICV
                     GAEFKEIFVSNTYFPDEGKI"
     gene            3743..4345
                     /locus_tag="P5241_01080"
     CDS             3743..4345
                     /locus_tag="P5241_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRV32166.1"
                     /translation="MTQTAPYYNFTKTLKQGSKGEEVKNLQSILANDNEITEKLPNLT
                     ISGTFDYTTYKALIMWQYKNGLKQTGIVDKDTLAKLNELYGRPTSESDIRQTSFNFTK
                     TLKINMKDAEVSDLQRVLSLDPSIYPEAQITGTFGALTKRAVERFQTKYGLPVTGIVD
                     EKTRSVLNSIYNKKTTSNIGAISGVNIYIEGTAFNLTPIT"
     gene            4708..5613
                     /locus_tag="P5241_01085"
                     /pseudo
     CDS             4708..5613
                     /locus_tag="P5241_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            5698..6741
                     /gene="mltG"
                     /locus_tag="P5241_01090"
     CDS             5698..6741
                     /gene="mltG"
                     /locus_tag="P5241_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRV32167.1"
                     /translation="MVSGKLIKNFLKHHYIIPLALGLLIVYFLFSALLSYPISLSHSQ
                     TFIVNPNDGFIEISNNLYKEKLIINPILFDIYVLLNGTYNKLKAGEYTFTSTINIKEI
                     AEHLYKGQNDFIVIPEGFNIFQIDAKLHQEHILDNNDSILQYTIGDFQNNNFKFLQEL
                     DPQKSLEGFLFPDSYVFIQNTPSKTIINIMLENFNTKVYKKYFLQTNPKDFYSNLILA
                     SILEKEILDDQEKQLGADILLRRLQNNIRLQTDAVLCYIEFLNNKDKTSETLCQNNIL
                     YLKSINSAYNIYKYNGLPPTPICNPGISSIFAILHPIQNNFYYYITDPITKKTIFAKT
                     LKEHNQNIQKYLK"
     gene            6874..8136
                     /gene="tyrS"
                     /locus_tag="P5241_01095"
                     /pseudo
     CDS             6874..8136
                     /gene="tyrS"
                     /locus_tag="P5241_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
     gene            8155..8613
                     /gene="def"
                     /locus_tag="P5241_01100"
     CDS             8155..8613
                     /gene="def"
                     /locus_tag="P5241_01100"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRV32168.1"
                     /translation="MIETGTNNPILRQKAKPIEKINKETSKLVNLMIQEIQNNNGVGL
                     AAPQIGQSVRVIVIQDPNTHKTYSFINPSIKRYSTKTALCEEACLSLPGISGMVERPQ
                     EIIIEARNLNNKKVKMKLKDMLSRIFQHEYDHLEGILFIDKAIVINKQTK"
     gene            8640..9263
                     /locus_tag="P5241_01105"
     CDS             8640..9263
                     /locus_tag="P5241_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRV32169.1"
                     /translation="MGNSHFSAIVLQNLLNNNFAPNLVITTSPKPIGRKQILTSNEVD
                     ILAQNNNIKIIYGDHLNDNVFLDTLKQEKIDLGVLASFGKIIPKTMIKLFPYGIINIH
                     PSLLPKYRGPSPIQTALLNGDTETGVTLFILSEGIDDGPIIGEVHQTIDPDDTFQTLS
                     KKLADIGATLAINILPEYKNGKVFVRTQKDSDATFTKKFTYKDGKIN"
     gene            9646..9864
                     /locus_tag="P5241_01110"
     CDS             9646..9864
                     /locus_tag="P5241_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRV32170.1"
                     /translation="MRIAINGFGRIGRSFFKQALEIPELEIIAINDLGDFNNLKYLLK
                     YDTVYGKFDTPIADSIKLLQEPDPSKLP"
     gene            10012..10428
                     /locus_tag="P5241_01115"
     CDS             10012..10428
                     /locus_tag="P5241_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32171.1"
                     /translation="MLGINESESQNFSIISNGSCTTNAISSILAILTQQPGIKKAILS
                     TTHAYTSTQKIIDSPSKKDFLRGRAGASNIIPASTGAANTITKVLSNLNNKFDGIALR
                     VPVICGSIADITFISAQPTNVEEINNILTQASLDIK"
     gene            10435..10557
                     /locus_tag="P5241_01120"
     CDS             10435..10557
                     /locus_tag="P5241_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32172.1"
                     /translation="MIAVSKEPLVSSDILKTTIPAIIDLSLTRVVDHDLVKIFS"
     gene            10746..11507
                     /gene="trpS"
                     /locus_tag="P5241_01125"
     CDS             10746..11507
                     /gene="trpS"
                     /locus_tag="P5241_01125"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRV32173.1"
                     /translation="MSLQEQYNCLYCVVDLHALTIPGNIKNKLAYQTYKLIALYLALG
                     LNPDKIHIFLQSENPNHTYLYWILECFTPFGQLKRMTQFKEKSAELKNIVSGGLFDYP
                     VLMASDILLYNTDLVPVGQDQKQHVELTRDIAENFNKFYKTNCFKIPEPLIPHTGAKI
                     KDLQNPQKKMSKSCNNDKGIIFITDTKTAIEHKISQAVTDSDGTIIFDPVSKPGISNL
                     IQIYSGFSGLSIKHIEQKYEHTTYDVFKKELALII"
     gene            11514..11666
                     /locus_tag="P5241_01130"
     CDS             11514..11666
                     /locus_tag="P5241_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32174.1"
                     /translation="MAPIQQKYAEYNKNTQYINKILDRGLEYSLSLSNNKITQIKTII
                     GLNRLK"
     gene            12204..13685
                     /locus_tag="P5241_01135"
     CDS             12204..13685
                     /locus_tag="P5241_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV32175.1"
                     /translation="MSENLIDRPPIVVIMGHIDHGKTTLLDYIRNSKIASKESGNITQ
                     HIGAYEITLEHENQQKRITFLDTPGHEAFVSIRSRGANLADIAILVIAADEGIMPQTK
                     EALFHIKAAKIPYIIAINKIDKQGINLEKLKNDLAKEEIYLEGRGGNIPYVEISAKTG
                     QNVNQLLELILLIAEMNELKGNPDQNAEGIVIESKIDSKRGICATLIITNGTIHINDT
                     IHIENYENKIKILEDFQSNAIQKATFSSPVLVVGFEKLPSPGAKFICNELTTKQKQSL
                     TQNTTQYKTKLVGDNQDIIIPVVIKADYVGSCEALEIALESLSKELNVSFNIIQNNVG
                     EICENDLKSINDKITTYLIGFRVKIAQSLINYIRNKPIKIILGETIYQILDQIKNDIL
                     LKNAPTQPTKTASLKVLAIFNDIKDDHLIGGELIEGEIYLKDTFKIFRVINDILQEIG
                     SGQIKNIQCQKQNVPHIGAPNQCGILVSSNTTFQQGDILEFYK"
     gene            13731..14093
                     /gene="rbfA"
                     /locus_tag="P5241_01140"
     CDS             13731..14093
                     /gene="rbfA"
                     /locus_tag="P5241_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRV32176.1"
                     /translation="MNKSTIPRNIKLANNLQSLISKIIEKEHRPIDNTIITVTEVVIN
                     PALTQANVFISVFPDTQAQQVLENIQKHIYTIQQQINKQCQIRTVPKIVFKIDTSPAK
                     IDKIEQLILEDKKRHTSK"
BASE COUNT         5488 a         2123 c         1911 g         4806 t
ORIGIN      
        1 gaaacgagat ttattaataa tacaaaatgt atatcactaa atctaaatta attgtttaat
       61 ctaaaccact tgactttttt taaaaaatag cttatactta taatatataa tcaaatatta
      121 aagtattaaa gagtcgaatt aataaaatat ttaaattttt taaaaatttt aaatttaata
      181 aattttcatt atgcaagata ataaaggatt tacattagta gagcttatga ttgttatagc
      241 tattttagcc atattggctg cagtggttgt ttttgcttta aacccagcac agcttatggc
      301 aagagccaga gattcacaaa gaatatctga tcttcaagcc ctaacaactg ctatcaatta
      361 ttttacagtt atttctccta ctccatattt agataatgct acaaattcca cctgcaaagg
      421 tatagcaaca actgctacta tatatgctac tgtaacaagt gctactgctc caggcggtgc
      481 ttttgcttat aacacaacag gtgttacaag tcgtgccatg gatggtagtg gatgacttaa
      541 agtaaatttt gctgctactg gtgaaacacc attagcccaa ttaccctgag acccatccaa
      601 tggtggagtt gctggttccc cgtcaactta ttatgccttt gtttgcagtt caactggtgc
      661 ttacgaaatt aatactagct tagaatcaaa ttcctacaaa cccaaagaag gtactgacgg
      721 tggcgatctg gctactgtat atgaagtagg aactagtcta actatagcac caagtgctac
      781 atcagcgggt tgattcccaa atgactaact attcataaga tgtcaatatt gacaaattga
      841 aataattttg tatacaaact atttaatcct accatagctt ttataagtat ggcaggatta
      901 attttatatt gaattatcaa aaaacgccct gcgtctttga aaataaccaa aataactttt
      961 tgaacagttt tatgttctct tatttttcaa tctttatatt tatcaatact acatttaata
     1021 ttatgatata atactccttg gggtcgtgct cttatgccac cagtcaatcc gtggtcatat
     1081 tctttacatt atgtttttgt tcaatactgt aaacgtaatg tatttactat tgttggggca
     1141 ttattattta tgtttatagc ttattggttt aataaaaaat ataatgaaaa atttttttac
     1201 aaagaagaaa tttatttaat gggtttgagc atgctttgca acccatggcc aggattaatt
     1261 gtcttactta ttattgtctt atttacgtct ttaattatcc aaatagttaa tttattttta
     1321 accaagcaca aacgtatatc aatgctatat ctatggttac cttgttctct attgtctttt
     1381 tttataactt atgctatact taaatatata cctattctaa atactttatt cgtttaaatt
     1441 atggcaatat ctttacaagt tttaaaacca attttattac aaagtggtct tgtatcagaa
     1501 gatactttta ataaagcact cgaaacctca aaacgtaccg gacaaaatgt cacggatatt
     1561 ttgatttcta atggatctat tactgcagat tatctaacag aaattatttc taattattta
     1621 aaaattcctc aagttcgcct acgtagtaaa gatattgact atgatattct taatattgtt
     1681 ccagaagata tagcccgtac tcgaaatgtc gctatttttg gcaaagacaa agaatactta
     1741 ttggttgcta tgacagatcc aactgacctt gaaacaatag aatttttaca acgttatacc
     1801 aatcatccaa tcaaagttta tattaccact gatgacgatc ttaaaagttc ttttgctttg
     1861 tatcgtcaaa aaattgccga aaatttcaaa aatataattg aagacaatat aaaacaagcc
     1921 gctaaaatag caggtatttc tttagaacaa gcagctgttg aattacctat tgttgcttta
     1981 tttaataaca ttgttgaata tgccgcttcc ttaaatgcca cagatattca tattgaacgt
     2041 ttgaatgatg ctgttttgat tcgttttcgt attgatggat tactcaaaga aattattcgc
     2101 ttaccaaaag aaacacatcc agctataatt gcccacatca aaattttatc taatttacaa
     2161 atcgatgaac ataataaacc acaagatggg cgttttaaat atcaaagagg agaagaagta
     2221 tttgatattc gtgtatctat actacccaca atgtatggag aaaaagctgc tatgcgttta
     2281 ttattaggag ctctaaaacc tttaagcttt gaagaacttg gtatgcaacc atatagcaca
     2341 gaaattataa atcaaaatat caaaagaagt tatggtatga ttttagtaac aggtccaacc
     2401 ggttctggta aaactacaac tttgtattct atattaagta aacttaatcg tccagaagta
     2461 aatgtagtaa ctatagaaga tcctattgaa tatgaaatga aatatatcaa tcaaattcaa
     2521 gtgaatgaga aatctggatt aacttttgcc aatggtctta gagctattgt ccgtcaagac
     2581 ccaaatatta taatggtagg tgaaattcgt gatggagaaa ctgctgatat tgctgttaat
     2641 gccgctttaa ctggacactt agtactttct actttacaca ctaatgatgc taccacagct
     2701 attcctcgtt tatttgatat gaaagtagaa ccatttttgg tagcggctac tataaatgct
     2761 atattagcac aacgtttggt aagacgtatt tgtaaagatt gtataacaag tattccagta
     2821 gatcaaaata tcaaagatct aattaaaaaa tattatctcg acagccctta taaaaaaact
     2881 aaagcaatta ttcctaacca attatataaa ggcaaaggat gcaaatcttg taattttact
     2941 ggctacaaag ggcgtttagc tatatatgaa attttgaata ttaatgaatc aattcgtacc
     3001 ctaattcaat catctcgttt taatttagat actcttaggg aagcagcagg aaaaactaat
     3061 atgataacaa tgttcgaaga tggtttaata aaagccgaac tcggattgac tactattgaa
     3121 gaaatattaa gagttattaa agaataaata caaaaaatta caaaattaaa aattgtattt
     3181 tattatgata tcacaacgtc tttgaaaaca aataaatgtt ttatttttta ctttattatt
     3241 tttaagttta tctggctatg gtttttatat tggtgtttta aaaccaaaac caaattgttt
     3301 tgataaaatt caaaaccaag gagaggcaga aattgattgt ggagggccat gccaatcttg
     3361 tgaaattaat catttaaaac cattagagtt ttctcaaaca ggtaattatg ttgtacaaaa
     3421 taataaatat tttctatata ctcgtgtttt aaatacaaat aaagattatg gcgccaaaac
     3481 ttttaaatat acttttactg ttactttgga agatggtgat actcaaactt tacaaggaat
     3541 tgattatatt ttgccttcat cgggaaaata tgtattgcta acaaatattt ccttgaattc
     3601 tgcccctaca caaataacat ttaatattga tacaaattca attgtttgat ctaccccgat
     3661 attaactaat ataccttcta aaatattctc tctaactaat ataagtttaa aaaagggtga
     3721 aaatattacc aatgccactg aaataacgca aacagcccca tattataatt ttacaaaaac
     3781 attaaaacag ggctcaaaag gagaagaagt aaaaaatttg caatcaattc ttgccaatga
     3841 caatgaaata actgaaaaat taccaaattt aactatttct ggaacttttg attatactac
     3901 ctataaagca ttaattatgt ggcaatataa aaatggtctc aaacagactg gaattgtaga
     3961 taaagatact ttggcaaaat taaatgaatt atacggtcgc cctacctctg aatcagatat
     4021 tagacaaaca tcttttaatt ttacaaaaac cctcaaaatt aatatgaaag atgctgaagt
     4081 aagtgattta caaagagttt tatcgcttga tccatccatt tatccagaag ctcaaataac
     4141 aggcacattt ggtgccttaa caaaaagagc tgttgagcgc tttcaaacaa aatatggatt
     4201 accagtcact ggtatagtag atgaaaaaac ccgttctgta ttaaatagta tatataacaa
     4261 aaaaaccaca agtaatatag gggcaattag tggtgtaaac atttatattg aaggcacggc
     4321 ttttaatctt actcctatta cttgaaaaac tgcttatata ataggcttta tttgtaatag
     4381 tgcagataat gttgtgggag tttctaaata taaatttgac aattttacag ccaattcttc
     4441 aagaactttt ttcttgtctt gaaatcataa tttaccccct gatttaaaaa tatgccctga
     4501 tggtcttaat atagatacta atatattaga taaaacgaat tgagtaaaat aatgataaat
     4561 aaatttttca aaatagattt ttatctattt ttacctatta tagttttact ttctctggga
     4621 cttttaattt tagcaagtct tggttctttt tgaaggcaaa taacatttat tattatagct
     4681 tttatttttt atattatttt tgcgaatatt gactatcaaa tttttcgtaa ttattcattc
     4741 atttcagtag tcatatattt aataggtgtt ttatcgctta ttttagtatt gatatttggt
     4801 ccaattactc atggcattac tggatggttt aactttggat tttttagtat ccaaccagta
     4861 gaatttatga aatttgcttt aattttgatt ttgtctaaat ttttagctac ccgtaatact
     4921 gatattatac attacaaaaa tattattgtt tcttttatct atgtgttttt accttgtgta
     4981 ttagttttgt tgcaaccaga tttgggttct ttaattatta tgctaggtat ttgattggga
     5041 ttacttttgg tatctggatt aaatttcaaa tattttctta taattttact atgtttttta
     5101 attgtaggag gattaggttg aaaatttatc ctaaaagact atcagaaaaa tcgtattatt
     5161 tcttttttaa acccattaaa tgatccaaga ggcaacaatt ataatttaaa acaagctatt
     5221 atagctatcg gaagtggtaa attattgggt aaaggagcag gatgaggtac acaaacacaa
     5281 cttaaatttt tacccgaagc caaaactgac tttatttttg cctctcttgc cgaagaaaca
     5341 gggtttttgg gtatatctat tttatttata tgtctatttt tactctttaa aagattatac
     5401 gaatatacca aacaagcaaa taataatttt gctcaattat ttattattgg gtttatgata
     5461 aaattagcaa ttgaagttat tattaatatt ggtgttaata ttgggctttt gcccgttgtt
     5521 ggtattgcct taccattttt aacattaggt ggatcacatt tagttgcaga ttttataatg
     5581 ctcggcatta tcgccaatat acattattat taaaaattca aaattaaaaa taaaaaaatc
     5641 cgaaagttcc aaattttaat ttcaaatttt taactttcat tagtaaaaat tacaaatatg
     5701 gtatctggaa aattgataaa gaatttttta aaacatcatt atattattcc tttggcattg
     5761 ggcctactta ttgtatattt tttattcagt gcacttttgt cgtacccaat cagtttatcg
     5821 cattcacaaa cttttattgt taatcccaat gatggattta ttgagatctc aaataatcta
     5881 tacaaagaaa aacttattat caaccctata ctatttgata tttatgtttt attaaatggg
     5941 acatacaaca aacttaaagc aggggagtat acttttactt ctacaataaa tataaaagaa
     6001 atagccgaac atctttataa aggacaaaat gattttatag ttattcctga gggttttaat
     6061 atattccaaa ttgatgcaaa attacaccaa gaacatattc ttgacaataa tgattctatt
     6121 ttacaatata ccataggaga tttccaaaat aataatttta aatttttaca agaacttgat
     6181 ccgcagaaat ccttagaagg atttttgttt cccgattcat atgtttttat acaaaacacc
     6241 ccctccaaaa ctattataaa tataatgtta gaaaatttta ataccaaagt atataaaaaa
     6301 tattttttgc aaacaaatcc taaagatttt tatagtaatt taattttggc atctatttta
     6361 gaaaaagaaa ttttagatga tcaagaaaaa caattaggtg cagatatttt gcttagaaga
     6421 ttacaaaata atatacgact gcaaactgac gctgtattat gctatattga atttttgaac
     6481 aataaagata aaacatccga aaccttatgc caaaataata ttttatatct caaatctata
     6541 aattctgctt acaatatata taaatataat ggcttgcccc ctacacctat ttgtaatcct
     6601 ggtattagta gtatatttgc tattttacac cctatacaaa ataattttta ttattatatc
     6661 accgatccaa taacaaaaaa gactattttt gccaaaacac ttaaagaaca taaccaaaat
     6721 attcaaaaat atctaaaata aaaatgtgtc tacatattcg tatgccaaat accccaaatc
     6781 tctattcaaa agtcttaaaa aaataagttg atttttacta taaattatgt tattataata
     6841 atagtaatta ttatacaaaa ctaaactaag tttatggatt tattggacga tttacaatgg
     6901 cgtggtttaa tatatcaaac cactgatatc gattcactac gcaaactatt acaacataat
     6961 aaaataacat tttatgaagg atttgatccg agttgtgaca gcttacagct tgggaattta
     7021 ttaacattaa ttactgccaa acgttttcaa atagctgggc ataaacctat tatacttgtt
     7081 ggtggagcaa caggtatgat aggcgatcca agtgataaaa aatctgaacg taaactacta
     7141 aacgaacaaa ctgtcaaaaa caataccaaa aaaatcgcat cacaaataaa acacatattt
     7201 aattcttttg attctaaaca catctctaat aaagtcaaaa ttgttaataa ttatgattga
     7261 tttaaaaaca taacattttt gacttttatg cgcgatattg gtaaatattt ttctataaat
     7321 tatttattat ctcgtgaatg agttaaaaca cgtttagatt caggcatttc ttttgctgaa
     7381 tttggatatt ctttgattca agcatatgat tattttattt taaatctcaa atataattgc
     7441 caattagaaa tagggggtag tgatcaatgg ggtaatatta ttgctggtgt ggatttaata
     7501 aaaaacaaaa ctcataaaga agtttatggt ttatctattc cgttgttatt acattctgat
     7561 ggcactaaat tcggtaaaag tgttcagaat tgtatttatc tagatccaaa caaaactact
     7621 ccttatcaaa tgtatcaata tttagttaat atcgacgatc gtgatattat acatttttta
     7681 aaacaattta cttttctaag tatcaaagaa attgaacaat atgaaacaaa aataatacaa
     7741 aacccagaac ttcgtgaagc acaaaaattt ttggcaaaat ttattgttaa actaatacat
     7801 ggcacaaaag ccgtgtcaaa atgtgaaaaa atctctaatg ctttatttta tggagatttt
     7861 caaaaactaa ctcaaaaaga acttgaacag ggactaaatg atgtaccaac ttttgaaata
     7921 attgatcata aacaaattaa cgtaattgat ctgcttgtat cagggaatat ttcatcttca
     7981 aaaagacaag caaaagaaga tataataaac aaagctatta ctataaatgg caaaaatata
     8041 tcggatatag aattgcaact aacaaaaaaa gattgcttat tcaatactta tctaattatt
     8101 cgtcgaggta aaaaaactta ttttttaggc aaatgaattt aaaacaaatt taatatgata
     8161 gaaacaggaa ccaataatcc aattttacgc caaaaagcca agccaataga aaaaatcaac
     8221 aaagaaactt caaaacttgt taatttaatg atacaagaaa tacaaaataa caatggggtt
     8281 ggtttagcgg ctccccaaat agggcaatct gttagggtta ttgttattca agacccaaat
     8341 acgcacaaaa catatagttt tattaatccc tcgatcaaac gttattcaac aaaaacagca
     8401 ctttgtgaag aagcctgttt aagtttacca ggaatatctg gtatggttga acgacctcaa
     8461 gaaataatta tagaagctag aaatttaaac aataaaaaag taaaaatgaa actaaaagat
     8521 atgttatcac gtatttttca acatgaatat gatcatttag aaggtatttt atttattgat
     8581 aaagccattg taattaataa acaaacaaaa taaaaatatt tattatgaat ttttgttttt
     8641 tggggaattc tcatttttct gccattgtac tacaaaattt attaaataat aattttgctc
     8701 ccaatttagt tattaccaca agccccaaac caataggacg aaaacaaata cttaccagta
     8761 acgaagtaga tattttagcg caaaataaca atataaaaat tatatacggt gatcacttaa
     8821 atgataatgt ttttcttgat actttaaaac aagaaaaaat agatttgggt gttctagctt
     8881 cttttggtaa aattattcct aaaacaatga taaaattgtt tccgtatgga attattaata
     8941 ttcatccgtc tctattacct aaatataggg gtccttctcc aatacaaaca gcactattaa
     9001 atggtgatac tgaaactggc gttactttgt ttatactctc cgaaggaata gatgatggtc
     9061 ccattattgg agaagtccat caaacaatag acccagatga tacttttcaa accttatcaa
     9121 aaaaattagc tgatatagga gccacactag caataaatat tttaccagaa tacaaaaatg
     9181 ggaaagtttt tgttcgcact caaaaagatt cagacgctac tttcactaaa aaatttacat
     9241 acaaagatgg taaaatcaat tgaaataata cttcacaagc aatttataat caaatacgtg
     9301 cactttctca cgaaccaggt acttattgtt tttttgataa aaataaaaaa caacaaactt
     9361 taaaaattca tcaagccaat ttaataatac cagacaaact tcttattgat atatctccaa
     9421 gcaccccggg tactgctatt tttcataata accatttttt aataagttca actgacggtt
     9481 taatagattt gttattagtt cagcccgagg gcaaaaatat tatgtctggc cctgcttttt
     9541 gacatggtaa taaaatatct ttattgtact aatagatctt tactttattt taatgatatg
     9601 ctataataat aacaacaatt taaacaacta ttaatataca atattatgcg cattgctata
     9661 aatggttttg gcagaattgg tcgcagtttt ttcaaacaag cccttgaaat acctgaatta
     9721 gaaataattg ctataaatga tttaggagat tttaataatt taaaatatct tttaaaatat
     9781 gatactgttt atggtaaatt tgatacacca attgctgatt caataaaact attacaagaa
     9841 ccagatccat ctaaattacc gtgaaaagat ttacaaatag atgtagtaat tgaatccact
     9901 ggtatatttg atgactatac caaagcccag tctcatttat cagcaggagc aaaaaaagtt
     9961 attattactg ccccagccaa aggtaacgaa ggtatagatg gcaaaactat tttgttaggt
    10021 ataaacgagt ctgaatctca aaacttttct attatttcca atggctcttg tactacaaat
    10081 gccattagct caatattggc aatattaaca caacaacctg ggatcaaaaa agcaattctc
    10141 agcaccactc atgcttatac ttcaacacaa aaaattattg atagcccaag taaaaaagat
    10201 tttttaagag gcagggcagg agcttcaaat atcatcccag cttctacagg tgctgctaat
    10261 acaataacca aagttttaag taatcttaat aataaatttg atggtattgc tttacgtgtt
    10321 ccagtaattt gtggctctat tgctgatatt actttcattt cagcccaacc cacaaacgta
    10381 gaagaaataa ataacatttt aactcaagca tccttagata ttaaatgaaa taaattgata
    10441 gctgtatcaa aagaaccatt agtatcttct gatattttaa aaaccactat acctgctata
    10501 atagatttaa gtcttacccg agttgtagat catgacttag taaaaatctt ctcttgatat
    10561 gataatgaat gagcttatgc tcatacttta attttacaac ttcttaatat gttaaaataa
    10621 ataatatctt tatttaatat atgacaaaaa taaataatca aaataatcaa aaaatagttt
    10681 tttctgggat acagccaaca ggagatttaa acatcggcca ttatttagga gccattaaat
    10741 cctgaatatc tctacaagaa caatataatt gcctttattg cgttgtagat ctacatgctc
    10801 ttaccatacc tggaaatata aaaaataaac tagcatatca aacttataag cttatagctt
    10861 tatatttagc gcttggacta aacccagata aaatacatat ttttttacaa tctgaaaatc
    10921 ctaatcatac ttatttatat tggatacttg aatgttttac accatttggg caattaaaac
    10981 gaatgactca atttaaggag aaatcagcag aactcaaaaa cattgtgtcc ggaggactat
    11041 tcgattatcc tgtcttaatg gcgtctgaca tattgcttta taatacagat ttggtaccag
    11101 tcggtcaaga tcaaaaacaa catgttgagc taaccagaga tattgctgaa aattttaata
    11161 aattttataa aactaattgt tttaaaatac cagaaccatt aatcccacat acaggagcta
    11221 aaatcaaaga tctccaaaat cctcaaaaga aaatgagtaa aagctgtaat aatgacaaag
    11281 gtataatttt tattacagat acaaaaacag caatagaaca taaaatatcg caagctgtta
    11341 ctgattctga tggaacaata atatttgatc ctgtatcaaa accgggaata tcaaatttaa
    11401 ttcaaatcta ttctggtttt agtgggctaa gtattaaaca tattgaacaa aaatacgagc
    11461 atacaaccta tgatgtcttt aaaaaagaat tagcattgat tatatgaaat tttttggcac
    11521 caatacaaca aaaatatgca gaatacaata aaaacacaca atatattaat aaaattttag
    11581 atagaggttt agaatatagt ttatctttat caaataataa aataacacaa atcaaaacaa
    11641 taatagggtt aaataggctt aaataaaaca ttatatatta tgactgataa atatttttac
    11701 cacattctgt ttataatttt aatagtatat gttgcttgag tagcaatata tgttacttat
    11761 agtctggtac aaataaatca aagagctata ggtagacaat ggttttttta tacattagaa
    11821 gataaagtat acaaattaaa aattgctgac aatgctatat cttgagaaaa aggtctgtct
    11881 ggtattcaaa aaaagccaga taattatgat ggcatgattt ttgtatttcc taataagcaa
    11941 attagacctt tttgaaacaa gggaacatat ttagatctac atgttatttg actaaaagac
    12001 tttcaaattg ttcaggaaga tgaactgccc tctataaaaa acggactcaa aattataact
    12061 cccaaaattc ctgttaatta tgttatagaa ttatttaaat aaaaactaaa ataaaaaact
    12121 caaataatac aaaaattctt gacattttat catttttggt gtagaattaa tatattaaat
    12181 acataataat ataaaaaatt aaaatgtcag aaaatttaat tgatcgccct cctattgtag
    12241 taattatggg tcatattgac catggtaaaa caactctttt agattatatt agaaactcaa
    12301 aaatagcctc aaaagaatca ggtaatatta ctcagcatat tggtgcctat gaaattacct
    12361 tggagcatga aaatcaacaa aaaagaatta cttttcttga tactcctggc catgaagcct
    12421 ttgtttctat acgttcacgt ggtgctaatt tggctgatat agccatttta gttattgctg
    12481 ctgacgaagg tataatgccc caaaccaaag aagcgctctt tcatataaaa gctgccaaaa
    12541 ttccctatat tatagctatt aataaaatag acaaacaggg tattaattta gaaaaactaa
    12601 aaaatgattt agcaaaagaa gaaatttatt tagaaggtag aggaggtaat attccttatg
    12661 tagaaattag tgccaaaaca ggacaaaatg taaatcaact attagaatta attttactta
    12721 tagctgaaat gaacgaatta aaaggtaatc cagaccaaaa tgccgaagga attgtaatag
    12781 aatcaaaaat agattccaaa agaggcatat gcgccacttt aattattact aacggcacta
    12841 ttcatattaa tgatacaatt catatagaaa attatgaaaa caaaataaag attttagaag
    12901 actttcagag caatgctata caaaaagcaa cattttcatc tccggtcctt gttgtaggtt
    12961 ttgaaaaatt acctagtcca ggagctaaat ttatttgcaa cgaactaaca accaaacaaa
    13021 aacaatcgct tacccaaaac acaactcaat ataaaaccaa attagttggc gacaaccaag
    13081 atattataat tcctgtagtt ataaaagctg attatgtggg atcttgcgaa gctttagaaa
    13141 tagctcttga aagcttatca aaagaattaa acgtctcttt taatatcatc caaaataatg
    13201 ttggagaaat ttgtgaaaat gatctcaagt ctataaatga taaaataaca acctatttga
    13261 ttgggtttag agttaaaata gcgcaatcct taataaatta tatccgcaac aaacctatta
    13321 aaattattct cggagaaact atttatcaaa ttttagatca aataaaaaat gatattttac
    13381 ttaaaaacgc tccaacacaa ccaacaaaaa cagcatcatt aaaagttttg gctatattca
    13441 atgatattaa agacgatcat ttaattggag gagaacttat tgaaggagaa atttatctaa
    13501 aagatacatt taaaattttt cgcgtcataa acgatatttt acaagaaatt ggcagtggcc
    13561 aaataaaaaa tatccaatgc caaaaacaaa atgtaccaca cataggtgca ccaaatcaat
    13621 gcggcatatt agtaagctct aataccacat ttcaacaagg tgatatttta gaattttata
    13681 aataacttct gtggcttgat tcttttataa ttttttgcta atattatatt atgaacaaat
    13741 ctactatccc acgcaacata aaattggcaa ataatctcca atcattaatt tccaaaatta
    13801 ttgaaaaaga acacagacct atagacaata ctattattac tgttaccgaa gtagttataa
    13861 atccagcttt aactcaagct aatgttttta tttctgtttt tcctgatacc caagctcaac
    13921 aagtattaga aaatatacaa aaacatattt atacaattca acaacagatc aataagcagt
    13981 gtcaaataag aacagtgcct aaaatagttt ttaaaataga caccagccca gcaaaaatag
    14041 ataaaattga acaattgatc ttagaagaca aaaaacgcca tacatccaaa taatgtgttt
    14101 gtaaaaaata taataaaaaa gataaaaaca ccatacatca tataaataat gtgcttaaaa
    14161 tataataaaa aacaaaatac acaatacaaa acaggacagt ctctaataaa agataaaaac
    14221 accataaagc tgaataatgt gcttaaaata taataagaga aattcaaata atgtgtttgt
    14281 aaaaaatata ataaaaaaga taaaaacacc ataaagctaa ataatgtg
//
LOCUS       DAOTJA010000009        13986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_4798_length_13986_cov_6.188070, whole genome shotgun sequence.
ACCESSION   DAOTJA010000009 DAOTJA010000000
VERSION     DAOTJA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4798_length_13986_cov_6.188070"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..733
                     /gene="lepB"
                     /locus_tag="P5241_01145"
     CDS             281..733
                     /gene="lepB"
                     /locus_tag="P5241_01145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRV32177.1"
                     /translation="MKGFKSFLLEFGEVIITALLIVLPIRMFVISPYFVKGQSMEPNF
                     HNNDYLLVDKLSYRFTEPKRGEVIVFKAPDGASYYIKRIIGLPGETIEINNNSIVVFN
                     KEFPEGLKLNEEYLPADDLTLNNMTIKLRENEYFVLGDNREASFDSRS"
     gene            893..1756
                     /locus_tag="P5241_01150"
     CDS             893..1756
                     /locus_tag="P5241_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRV32178.1"
                     /translation="MSKKSIRFVDIKKKLNKEKTDKLNKGKTRKNICQAPQGMHDIVF
                     DEKKIFDKVVQEIYNIACFYGYEQIITPILEYGDLFVYGLGKDNKDLNGMFWIKNCKN
                     KKLALRLEGTIPIVRAFLEHNMSLFLQPTKIFYIGPFFNKTELYKAGFEIIEDDNIIN
                     DVELIWLSKIILQDLNLDNVRIELNSIGCPKCFKNYEKAIKKYYRSKKNRLCASCRKY
                     LDSNILMLLNCQEENCRALRVEAPKIKDFLCTDCKNHLQNITQYLKDMHIPFVLNPEL
                     VLRDNYYNKTI"
     gene            1877..2239
                     /locus_tag="P5241_01155"
     CDS             1877..2239
                     /locus_tag="P5241_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV32179.1"
                     /translation="MVLNIYDIVNVLKTHKLLDVRNKIPNFFLMPVGETAKKKSFEIL
                     EELRKNDIKILFSFGKDSVRSQMKFADKIKTKYILLLGQEEVNRNEIILQEASSELQE
                     NIPLPKLIKVLKNKLNNA"
     gene            2249..2476
                     /locus_tag="P5241_01160"
     CDS             2249..2476
                     /locus_tag="P5241_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRV32180.1"
                     /translation="MSIKIQRKENESISTFIYRASQIIQKSGVLMEALKHQNHIKKPN
                     ERKKKLAALHRIKVLKDIEYKKKMGLPINKH"
     gene            2485..2895
                     /gene="ybeY"
                     /locus_tag="P5241_01165"
     CDS             2485..2895
                     /gene="ybeY"
                     /locus_tag="P5241_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRV32181.1"
                     /translation="MIKIEFLLYNFSNIKIIQQDLLKQYTTKIINTIIPLLNPNIKQV
                     SLAVVMLNLQDSYQLNKIWRKKRYVPVELSFANYSQQYLKKIKESSIILGDIILTPEL
                     LQTSQDYKTALIHSLLHLLGYNHNTQANYKKMQK"
     gene            2929..3480
                     /locus_tag="P5241_01170"
     CDS             2929..3480
                     /locus_tag="P5241_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRV32182.1"
                     /translation="MRQALLALDIGTNNIHIAVFKTNPNQIILNWNRPSVGVKRGVIC
                     NVDDVSACLDKVLDEVASALNTNVLKGIVCIGGNNIEMRKSKGIAIIANDNNEINKPD
                     IARAQQSSQSFALPVNRTLIHSIPQKFLVDGVEVSDNPVGMVGMRLEVESLIIDAFTP
                     EIRNLDNVLKNVGFKQDALLVNT"
     gene            3520..3693
                     /locus_tag="P5241_01175"
     CDS             3520..3693
                     /locus_tag="P5241_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HRV32183.1"
                     /translation="MGVIGIDLGAGTTSFSVFEEDKMIYTKVLPAGGNYISNDIALWL
                     TIPPNEGENIKIK"
     gene            3709..4035
                     /locus_tag="P5241_01180"
     CDS             3709..4035
                     /locus_tag="P5241_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32184.1"
                     /translation="MKVDKKEMLNLGDIVAGEDKNFSRRELAEVIQARLEEIFDFING
                     ELKQIGKYAKLPGGVVLYGGGANLPDIVELARDKLKLPARIAHPQFNGLIETDPSFVN
                     VVGAIQ"
     gene            4141..4605
                     /locus_tag="P5241_01185"
     CDS             4141..4605
                     /locus_tag="P5241_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32185.1"
                     /translation="MAQTASNNNFKSKPNIKIKIIGIGGGGGNILTRMYKRKVDGVEF
                     IALNTDAQALHFTKADKKINIGVNLTRGLGAGMDPEIGKKAAEESLQEIEEALSGADL
                     IFITCGLGGGTGSGAAPVVAEAARKTNALIIGVVTKPFFFEGTKRMDIANEA"
     gene            4624..5391
                     /locus_tag="P5241_01190"
     CDS             4624..5391
                     /locus_tag="P5241_01190"
                     /inference="COORDINATES: protein motif:HMM:NF023746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRV32186.1"
                     /translation="MDALITIYNDRIFNIIEKDTPILEAFKQIDEILRQGVQGISDLI
                     NFPGLINLDFANLQTILVNAGDSIIGIGKASGKDRTIKAAKAAISSPLLNLSIDGASR
                     ILFNISGHNDLTLSEITEAARIITDTADPAAKIIFGASYDNELKSGEVKVIVIAGGFG
                     QTKQPQEELFSNSSIPEIFSHQYNTAKQESDVFSLPSSYNFKDEVKNNVKKFEDKEKN
                     NLGNSDGEKIVSVDLTNEEKWDEKELEIPAFLRKRKK"
     gene            5502..7637
                     /locus_tag="P5241_01195"
                     /pseudo
     CDS             5502..7637
                     /locus_tag="P5241_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787711.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            7688..8386
                     /locus_tag="P5241_01200"
     CDS             7688..8386
                     /locus_tag="P5241_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRV32187.1"
                     /translation="MIFGGFQQLSLIDYPGKICAIAFTIGCNFRCAFCHNKELVLPEL
                     IAKQPIITEDDILTFLKTRIGKLEALCITGGEPSLYDDLDKFLQKIKQLGLLIKLDTN
                     GTNPTILSKILDAKLVDYIAMDIKAPLTTQDYNKITGVQVNLDNIKKSIELIKTKLSD
                     YEFRTTVEPSLREQDILQIAKYLAPAKKYFLQEFRTGNTLNPLYNSMKGLSQDELEKI
                     KDKIKDNFEQIGVR"
     gene            8508..9029
                     /locus_tag="P5241_01205"
     CDS             8508..9029
                     /locus_tag="P5241_01205"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRV32188.1"
                     /translation="MFLEEGLQIKQSSLLLKLNDFNYQKVDYVKFPGEFSHRGFIIDI
                     FSPSQEKPIRIEFSGNYIVKISFFSVQEQESIFEKQKNKLLKNLPELAGLEIGDFVVH
                     VDHGIGIFRGYQEVPVESKNLECQKYFIIEYAPPAPHKEPDKLFVPYDKANKLSKYYG
                     FENPTIHRLSTQT"
     gene            9063..10337
                     /locus_tag="P5241_01210"
     CDS             9063..10337
                     /locus_tag="P5241_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV32189.1"
                     /translation="MAFAQNLLNLYSKKEISKTEPLWGDAELEGILNSTFDFELTPDQ
                     VKAIQDIYKDLESGKLMDRLILGDVGFGKTEVAIRAAFRVVLHKKQVVLLAPTTILVK
                     QHEEVFKNRLERFGLSIVSMSRLNSKEQDKKNLSMIKSGKADIIIGTHRLLSSDIKFS
                     NIGMLIIDEEQRFGVKQKEKIRALNDKVHVLSLSATPIPRTLNIALSGFKKMSVIETP
                     PINRLPVQTYILPYKKPIVIETIQKEIERRGQVFYIHNRIVNIEQSVLDLKRELPKIK
                     IDFLHGRMSEKNIVKVMEKFKNQEIDVLVSTTIIENGLDLSNVNTLIVEDATRLGLGE
                     AYQLRGRIGRGELDSFAYFFYPKRLTNKAQMRLQALKEANYLGAGYQIALRDLEIRGA
                     GNILGRAQSGNINKVGYNLYCQMLNQAMHNNL"
     gene            10704..11213
                     /gene="nusG"
                     /locus_tag="P5241_01215"
     CDS             10704..11213
                     /gene="nusG"
                     /locus_tag="P5241_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRV32190.1"
                     /translation="MLHTAAGYEEAVARNLKSRIEAFDLGDKVFDVMVPIEKKIKIKN
                     GKRIVYEEKMFPSYVFVDMIVDDSSWYVVRNTPHVTGFIGAGSTPLPVDKKEIDIIFK
                     RIRGDEPHYKIDLSIGDRVKIIDGPFKDFEGKVQGVDTEKGRVKVLVGVFGRETPIDV
                     DFLQINKID"
     gene            11287..11709
                     /gene="rplK"
                     /locus_tag="P5241_01220"
     CDS             11287..11709
                     /gene="rplK"
                     /locus_tag="P5241_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRV32191.1"
                     /translation="MAHKPIKANIKLEIKAGAANPAPPVGPALGQHGVNIQEFCNTFN
                     NATKDKAGDIIPVEITIYEDRTFSLVFKEPPVASLILKEAGIEKGSANPLNKVGKITK
                     QQVESIAKRKMPDLNTTDLEAAMNIVEGTARSLGIDIV"
     gene            11767..12336
                     /gene="tmk"
                     /locus_tag="P5241_01225"
                     /pseudo
     CDS             11767..12336
                     /gene="tmk"
                     /locus_tag="P5241_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
     gene            12430..13329
                     /gene="gatB"
                     /locus_tag="P5241_01230"
                     /pseudo
     CDS             12430..13329
                     /gene="gatB"
                     /locus_tag="P5241_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
     gene            13471..13944
                     /locus_tag="P5241_01235"
     CDS             13471..13944
                     /locus_tag="P5241_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32192.1"
                     /translation="MLVKTSIESINNIEQNQNIIELCYNYLTVDFLGLIAKENKTFSK
                     DLLNPNNFALLIKYLIENKISSKGAKIIIENLVRDPNLNTEDILNNNNLLQVSDENFV
                     VELVQKVLLDNPSSVQEYKNGKESVLQFLIGQAMKASKGTANPQIVKEIMLKLLG"
BASE COUNT         5311 a         1597 c         2506 g         4572 t
ORIGIN      
        1 aaaaagattt attaatttgt ttttttattt aatttttatg ccaaaagtct aaaatatatc
       61 atagaataaa tatatataga agcaaaaagt tagagatttt aaattgaaag caatttttat
      121 gttatgcgac ttttaacttt ttatttgtcg cacctatttt ttaaaaatta aaaattggaa
      181 attcgaaatt attatataat aagggtcttg atatttagtt aattttatga tatactaata
      241 atatattgat aataataaat caagggatta tttaatatat atgaagggtt ttaaatcttt
      301 tttattagaa tttggagaag taataattac agcgctttta attgtactac caattagaat
      361 gtttgtaata agcccatatt ttgtcaaagg tcaaagtatg gagccaaatt ttcataacaa
      421 tgactattta ttagttgata aattatcata tagatttaca gagccaaaaa gaggcgaggt
      481 tattgtgttt aaagcaccag atggtgcgag ctattatatt aaacgaatta ttggtttgcc
      541 aggagaaaca atagaaatta ataataattc cattgtggtt tttaataaag aattccccga
      601 gggattaaaa ttaaacgaag aatatttgcc agctgatgat ttaacgctta ataatatgac
      661 aataaaatta cgagaaaacg aatattttgt attaggagac aatagagagg catcttttga
      721 ttctcgtagc tgaggtaatt tagaaagaaa taaaattatt ggtaaggtat gagtaagagt
      781 agcgcctatt agtaatgcca gagctattac tttgccagtt tataaataat atagattgat
      841 aagtaattta gatacaaaaa acaataaaat aaaacattta taagtcaaaa ttatgtccaa
      901 aaaatccatt cgatttgttg atattaagaa aaaattgaat aaagaaaaaa cagacaaatt
      961 aaacaaagga aagacgagaa aaaatatatg ccaagcacct caaggtatgc atgatattgt
     1021 ttttgatgag aaaaaaatat ttgataaagt agtacaagag atttataata ttgcttgttt
     1081 ttatggctat gagcaaatta ttactccaat tttggaatat ggagatttat tcgtgtatgg
     1141 gcttggtaaa gataacaaag atttgaatgg aatgttttgg atcaagaatt gtaaaaataa
     1201 aaaattagct ttaagattgg agggcactat acctattgtt agagcttttt tggaacataa
     1261 tatgtctttg tttttacaac caaccaagat tttttatatt gggccatttt ttaataaaac
     1321 agaactttat aaagcaggat ttgaaattat tgaagatgat aacataatta atgatgtaga
     1381 gttgatttgg ttatcaaaaa taattttaca agatttaaat ttagataatg tacgaataga
     1441 actaaatagt atagggtgcc caaaatgttt taaaaattat gaaaaggcaa ttaaaaaata
     1501 ttatagatca aagaaaaaca gactgtgtgc ttcttgtaga aaatatttgg atagtaatat
     1561 tttgatgctt ttgaactgtc aagaagagaa ttgcagagca ctaagagtag aagcacccaa
     1621 aattaaagat tttttatgta ctgattgtaa aaaccatttg caaaacatta ctcaatattt
     1681 aaaagatatg catattccat ttgttttgaa ccctgaattg gtgttgcgag ataattatta
     1741 taataaaact atttgaagtt ttgtttatga taatataatt tctgaggata ataaagaaac
     1801 tattgtttta gcctatggtg gcagacaaga taatttatta aaattattta ctgggcaaaa
     1861 caaaccgatt actggcttgg tattaaatat ttatgatata gtaaatgttt tgaaaacaca
     1921 taaattgttg gatgttagaa ataaaatacc taattttttt cttatgccag taggggaaac
     1981 agcaaaaaag aaaagttttg aaattttgga agaattaaga aaaaatgata ttaaaatttt
     2041 atttagtttt ggtaaagaca gtgttagatc gcaaatgaaa tttgcagata aaattaaaac
     2101 aaaatatatt ttattgttag gacaagaaga agtcaataga aatgaaataa ttttacaaga
     2161 agcttcttca gaactacaag aaaatattcc attacctaaa ttgataaagg tcttaaaaaa
     2221 taaacttaat aacgcatagt agaatattat gtctataaaa attcagagaa aagaaaacga
     2281 atctatttct acttttattt atagagcatc tcagataatt caaaaaagtg gagtacttat
     2341 ggaagctcta aaacaccaaa atcatattaa aaagcccaat gagcgcaaga aaaaattggc
     2401 agctttgcat agaataaaag ttcttaaaga tattgaatat aaaaagaaaa tgggattgcc
     2461 aattaataaa cattagaata aattatgata aaaatagaat ttttattata taatttttct
     2521 aacataaaga ttatacagca agatttatta aaacaatata caactaaaat aattaatact
     2581 attataccat tgttaaatcc taatataaaa caagtaagtt tggcagtagt tatgctaaat
     2641 ttgcaagatt cataccaatt aaataaaatt tggagaaaaa agcgttatgt ccctgtggag
     2701 ttgagttttg ctaattattc acagcaatac ttgaaaaaaa tcaaagaaag tagtataata
     2761 ttaggagata ttatcttgac accagaattg ttacaaactt cacaagacta caaaactgca
     2821 cttattcata gccttttaca tcttttggga tataatcata atacccaagc aaattataaa
     2881 aagatgcaaa aatgagagaa gtctttttta aaaaattaaa aatattttat gcgacaagct
     2941 ttattggctt tagatatagg gactaataat atacatattg cggtttttaa aacaaatcct
     3001 aatcagatta tattaaactg gaatcgtcct tcggttggag taaaacgagg agtaatttgt
     3061 aatgtagatg atgtatcggc gtgtttggat aaagtattgg atgaagtagc aagcgcttta
     3121 aataccaatg ttttaaaggg cattgtttgt attggaggta ataatattga aatgaggaaa
     3181 tcaaagggta tagctattat tgctaatgat aataatgaga ttaataagcc agatatagcc
     3241 cgtgctcagc aatcttctca gtcttttgct ttgcctgtca atagaacttt aatacatagt
     3301 atcccacaaa agtttttagt agatggtgta gaagtttcag ataaccccgt tggtatggta
     3361 ggtatgcgtt tggaagtaga aagcttaatt attgatgcat ttacgccaga aataagaaat
     3421 ttggataatg tattaaaaaa tgttggtttt aaacaagatg ccttattagt aaatacttga
     3481 gctggatctt atggtgcttt atctaaacaa gaaaaagaat tgggtgtaat tggtattgat
     3541 ttaggagctg gtactacgag cttttcggta tttgaagaag ataaaatgat ttataccaaa
     3601 gtgttaccag ctggaggtaa ttatattagt aatgatatag ctttatggtt aacaattcca
     3661 ccaaatgaag gagaaaatat aaaaatcaaa tgaggaagtg cttatgcttt gaaagtagat
     3721 aagaaagaaa tgttaaactt gggcgatatt gtagctggtg aagataaaaa tttttctcgt
     3781 agagaattag ccgaagtaat acaagctcgc ttggaagaaa tttttgattt tattaatgga
     3841 gagttaaaac aaattggtaa atatgccaaa ttgcctggag gggtagtgtt atatggtggt
     3901 ggagccaatt tgccagatat tgtagaattg gcaagagaca aattaaaatt gccagcgcgt
     3961 atagcccatc cgcaatttaa tggcttaata gaaacagacc cgagctttgt aaatgttgtg
     4021 ggggcgatac aatgatattt atatgatgaa tatgatcata atgctccaat acttaatagc
     4081 catggtttgg gtaatctaat ttcaaaactt tttaaaaatt ttaatcctta aatatataat
     4141 atggcgcaaa cagcttcaaa taataatttt aaatccaagc ccaatattaa aattaaaatt
     4201 attggtatag gtggtggagg cggtaatatt ttaacaagaa tgtataaaag aaaagtagac
     4261 ggagtagagt ttattgcctt gaatacagat gctcaggcat tacattttac taaagcagac
     4321 aaaaaaatca atattggggt taatttaacc aggggcttag gagcaggaat ggatccagaa
     4381 ataggtaaaa aagctgccga agagagttta caagaaatag aggaggcttt gagtggagct
     4441 gatttaattt ttattacttg tggtcttggt gggggtactg gatcaggtgc tgcacctgtt
     4501 gtggcagaag cagcgcgcaa gaccaatgcg ttaattattg gagtagtgac gaaaccattt
     4561 ttctttgagg gtactaaaag aatggatatt gccaatgaag cttgaaaaaa aatatacaac
     4621 aatgtggatg cactaattac aatttataat gatcgtattt ttaatattat tgaaaaagat
     4681 acacctattt tagaagcgtt taaacaaatt gatgagattt taagacaagg tgtacaaggc
     4741 atttctgatt taataaattt tccaggtctt attaatttag atttcgctaa tttacaaact
     4801 attttggtta atgccggaga ttcgattatt ggtattggta aagcttctgg taaagatcgt
     4861 acaataaagg cagccaaagc agcgatttcg tcgccattgc ttaatttatc tatcgatggt
     4921 gctagtcgta ttttatttaa tattagtgga cataatgatt taacattatc cgaaattacc
     4981 gaagcagcaa gaataattac tgatacagca gatccagctg ccaaaattat ttttggtgca
     5041 tcttatgata atgaattaaa gtctggagaa gtaaaagtaa ttgttattgc gggtggtttt
     5101 ggtcaaacaa aacaacccca agaagaatta tttagcaatt caagtatacc agagatattt
     5161 tcacatcagt ataatactgc aaaacaagaa agtgatgttt tttcattgcc ttcatcctat
     5221 aattttaaag acgaagtcaa gaataatgta aaaaaatttg aagacaaaga aaaaaataat
     5281 cttggcaatt cagatggcga gaaaattgta agtgtagact tgacaaatga agaaaaatgg
     5341 gatgaaaaag aattagaaat acctgccttt ttaagaaaaa gaaagaagta aaaataaata
     5401 agttgtcaat cttgctattt ttttaaacct ttgatatact atttaaactc gcatcaaagg
     5461 ttttttcttt ttattaataa aataattaaa attttattat gatgtcacct agcaaagctg
     5521 ttttggcgcc aaaagccaaa aaaactaata aaaatcatat taaattaatt aaaaataggg
     5581 aaggcgtggt aataaaatat gatatatcac gtattgaaga tgcagttttt agagcttttt
     5641 ctactaatga agaaggcaac aaaacagaag ccaaaaatat agccaaaaaa gttacattta
     5701 ttgttaatcg taggtttaaa caaggagaaa ttccaacagt agaagatgta caaaatatta
     5761 tagaagaagt tttgatattg caaggatatg tagaaaccgc aagaaattat attttatatc
     5821 gcgaacaacg tagaagattg cgtgaaagca aaattacgaa agaagaagcc attgatttag
     5881 taggaaatta tttagattcg acagattgag aagtcaaaga aaatagtaat atgaattatt
     5941 ctttacaggg attgaacaat tatatttcta ataatattac taaaagatat tggttaaata
     6001 aaatttatcc caaagaaata cgtgatgctt caaaaaataa tttgtttcat attcatgatt
     6061 tagggacttt gggcgcatat tgttgcgggt gagatctcta tgatttgttg tgtcgaggat
     6121 ttggaggcgt aaataataaa atacaatcaa aacctccaaa acattttcgg actgctttgg
     6181 ggcaaattat aaattatttt tatactttac aaggagaagt agctggtgcg caagcttttt
     6241 ctaattttga tactttatta gctcctttta ttcgttatga taatttaaac atccaagaag
     6301 taaaacaagc tttacaagaa tttttattta atgtaaatgt accaacacgg gtaggatttc
     6361 aaactccttt taccaatata actttggatt tacagatacc gagtaattat aaagatatgc
     6421 cagtagtgat tggtggacag ttgcaaaaag aaacatataa agaatttcag ccagagatgg
     6481 atgtgtttaa taaaatatta tgtgaggtat ttaccgaagg tgatgcgagt ggcagggtat
     6541 ttacttttcc tattccaaca tacaatatta ccaaagattt tgattggagt aatccaaatt
     6601 atgaaggtat ttggcttatg actgcaaaat atggattgcc atatttttcg aattttatat
     6661 cttcagatat gtcgcccgaa gacgccagat caatgtgttg tagattaagg cttgataaac
     6721 gcgagctgta taaaagaggt ggaggattgt ttggggctaa tcccaaaact ggaagtctgg
     6781 gagtagtaac agtaaattta ccaagaattg cgtatttatc caaaactaga aaagattttt
     6841 ttgcaaaatt aaaagaagta atggatttgg ccaaagaatc tttggaaata aaacgtaaaa
     6901 caattgaaca gtttactcta aaaggcttat atccatattg tcgatattat ttacaagatg
     6961 tttataaaat gcgtgggtct tattgggcaa atcattttgc tactattggt ttggttggtg
     7021 gtaatgaagc ttgtcaaaac tttttggggt gttcaattgc tgataaggaa ggtaagaaat
     7081 ttatggaaga agtattacaa tttatgagag acatattagt ggaatatcag gacgaaactg
     7141 gtaatttata taatttagaa gctacgccag ctgagggaac ttcatatcgt ttagccaaaa
     7201 ttgataaaga tgaatttccg gatattatta cagcaggtga ggatgcgcct tattatacca
     7261 attctacaca attaccagtt aattttactg atgatctgtt tgaggtattg gatcatcaag
     7321 atagtttaca agttgcttat actggaggta cagtagtcca tgggtttatt ggcgaggaaa
     7381 ttagagatcc atcttcagta aaaacattag taaaaacaat ttttagtaat tatcgtttgc
     7441 cttatatgac aattactcca acgtttagtg tctgtcctac acatggttat attgctggtg
     7501 aacattttac ttgtccaaaa tgtgtaattg aacaaccttg tgaagtttat tcacgtgttg
     7561 ttggttattt aagacctgtg cagagttggc atgttggtaa acaagaagaa tttaaacaaa
     7621 gattagaatt taattgaaat aaagtaataa acaaaggtgc tattgttaaa agttctaaaa
     7681 aataaatatg atatttggag ggtttcaaca attgtctttg attgattacc ctgggaagat
     7741 atgtgctata gcttttacta tagggtgtaa ttttaggtgt gccttttgtc ataataaaga
     7801 attagttttg ccagaactta ttgccaaaca gcctattatt accgaggatg atattttgac
     7861 ttttttgaag actcgtatag gaaaattgga agctttgtgt ataacaggag gagaaccaag
     7921 tctatatgat gatttagata aatttttaca aaaaataaaa caattagggc ttttgataaa
     7981 actcgatacc aatggtacaa atccaacaat attatcaaaa attttagatg caaaattagt
     8041 agactatata gctatggata ttaaagcgcc attaactacg caagattata ataaaattac
     8101 aggtgtacaa gtaaatttag ataatattaa aaaaagtatt gagcttataa aaacaaaatt
     8161 gtctgattat gaatttcgta ctactgttga gccctcactt agagaacaag atattttaca
     8221 aatagctaaa tatctagcac ctgcaaaaaa atatttttta caggaattta gaactggcaa
     8281 tacgttaaat cctttgtata attctatgaa aggtttgagc caagatgaat tagaaaaaat
     8341 taaagataaa ataaaagata attttgaaca aattggagtg agataaaaat tatttgattg
     8401 tcgcattatc cccttatttt ttagaaaggg ggcctgtttg atttaaagaa aattttgata
     8461 atatatacaa tgctgctaaa acccaaaatt tttgacataa caatgttttg tttttagaag
     8521 aagggttgca aataaaacaa tcaagtttgc ttttaaaatt aaatgatttt aattatcaaa
     8581 aagttgatta tgtaaaattt cctggtgaat tttcgcatag gggatttatt attgatattt
     8641 tttcccccag tcaggaaaaa ccaattcgta tagagtttag tggtaattat attgttaaaa
     8701 tatcgttttt tagtgtccaa gaacaagaaa gtatatttga gaaacaaaaa aataaattat
     8761 taaaaaattt acctgaattg gctggtttag aaattggaga ttttgtagta catgttgatc
     8821 atggcatagg aatatttcga ggatatcaag aagtacctgt agaatcaaaa aatttagaat
     8881 gtcaaaaata ttttattatt gaatatgctc ccccagcacc ccataaagaa cctgataaac
     8941 tttttgtgcc ttatgataaa gcgaataaat taagcaaata ttatggattt gaaaacccga
     9001 caattcatcg tttgtctact caaacttgat ttaataccaa aaagaaagta aaggaagatg
     9061 ttttggcttt tgcccaaaat ctattgaatt tatattccaa aaaagaaata agcaagactg
     9121 agccattgtg gggagacgct gaattagaag ggatattaaa tagtactttt gattttgaat
     9181 tgacaccaga tcaagtaaaa gccattcagg acatatacaa agatttagaa tcaggtaagc
     9241 ttatggatag attgattttg ggagatgttg gttttggaaa aactgaagta gccatacgag
     9301 ctgcttttag agtggttttg cataaaaaac aagttgtact tttggcaccc actactattt
     9361 tagttaaaca gcacgaagag gtatttaaaa atagattaga gcgttttgga ttatctatag
     9421 tttctatgtc tcgactgaat agtaaagaac aagataaaaa aaatttatca atgataaaat
     9481 caggtaaagc tgatattatt ataggtactc atagattatt gagtagtgat attaaattta
     9541 gtaatatagg aatgttgatt attgatgaag agcaaagatt tggtgtaaaa caaaaagaaa
     9601 agattcgggc attgaatgat aaagtccatg tgttgagttt gtcagccaca cctattccaa
     9661 gaacattaaa tattgcttta tctggattta aaaaaatgag tgttatagaa actccaccta
     9721 ttaatagatt acccgtgcaa acatatattt tgccttataa aaaaccaatt gttatagaaa
     9781 caatacaaaa agaaatagaa cggcgaggac aggtttttta tattcataat agaatagtta
     9841 atatagaaca gagtgtattg gatttgaaaa gggaattgcc gaaaataaaa atagattttt
     9901 tgcatggtag aatgtcagaa aaaaatattg taaaagttat ggaaaaattt aaaaaccaag
     9961 aaatagatgt tttagtatct acgactatta ttgaaaatgg gcttgattta tcaaatgtca
    10021 atactttaat tgtcgaagat gctacacgat tgggtttagg cgaagcctat cagctaagag
    10081 gaagaattgg taggggcgag ttggattctt ttgcttactt tttttatccc aaacgtttaa
    10141 caaataaagc ccaaatgagg ctgcaagctt tgaaagaagc gaattatttg ggcgcaggat
    10201 atcaaattgc tttgcgtgat ttagaaatac gcggagcagg taatatatta ggtagggctc
    10261 agtcaggtaa tataaataaa gtcggatata atttatattg tcaaatgtta aatcaagcta
    10321 tgcataataa tttataggta tatagagatc ttgatattta actaattttg tgatagtata
    10381 taattatata cgtatttagt tacaaaaacg attaaattgc atacaaaatg ttcaaaaaaa
    10441 taaaaacctt ttttagagag tcttttttag aactcaaaaa agttcattga ccaactccca
    10501 aagaaaccaa aaatttaaca atagctgttt tagtattggc ggcttttttt ggtttgattt
    10561 tggctttaat ggattttggt ttaggtaaaa taatttatcc tttaatcatt aaataagcaa
    10621 aattatttag aaaattgatg tttaaagttt aatatattaa tattatggca aaacaagaac
    10681 tggatttagg tagaaattga tatgtgttgc atacagctgc gggttatgaa gaagctgtag
    10741 cacgtaattt gaagtctcgt atagaggctt ttgatttggg tgataaagta tttgatgtaa
    10801 tggtaccaat tgaaaaaaag atcaaaatta aaaatggtaa gagaattgtt tatgaagaaa
    10861 aaatgtttcc ttcttatgta tttgtagata tgattgttga tgatagctcg tggtatgttg
    10921 tgcgtaatac tccccatgtt actgggttta ttggtgctgg atctactcct ttgcccgtag
    10981 acaaaaaaga aattgatatt attttcaaaa gaattagagg agacgagcct cattataaaa
    11041 ttgatttgtc tataggcgat agagtcaaaa ttattgatgg cccatttaaa gattttgaag
    11101 gcaaagtgca gggtgttgat actgagaaag gaagagtaaa ggttttggtg ggtgtattcg
    11161 gaagagaaac tcctattgat gtcgattttc tccaaattaa taaaattgat taatataaaa
    11221 tatatttaat aaaaatcctt gtatagtcaa gggacaaaat attttttgtc gtataaatta
    11281 tatataatgg cacataaacc aataaaagca aatataaaat tagaaattaa agcaggcgct
    11341 gctaaccctg ctcctccagt tggtccagca ttaggacagc atggagttaa tattcaagag
    11401 ttttgcaata cttttaataa tgctactaaa gacaaagcag gtgatattat tccagtagaa
    11461 attacaattt atgaagatcg tactttttca ttagttttta aagaaccacc tgtggctagt
    11521 ttaattttaa aagaagctgg tattgaaaag ggttcggcta atcctttgaa caaagtaggc
    11581 aaaattacta aacagcaagt agaatctatt gccaaaagaa aaatgcctga tttgaatact
    11641 acagatttag aagcggctat gaacattgtt gaaggtacag ctcgcagtct tgggatagat
    11701 attgtataaa ttatcgatta aaaatttaga aaaattaaca aagacatttt taaaatatca
    11761 ttcattatga tttctaaaaa aaataaaggc tgtttaattt ttttagaagg tagtgatttt
    11821 tctggtaaaa ctactcaaag cgagttgttg tttaaatatt taaaacaaca agggttgcct
    11881 gttgtcaaga ctaaggagcc tggagcaaca aatttaggaa agcaaatacg tcagattctt
    11941 ttattttcta aagaaaagat agattgtaaa gcagaagctt ttttatttag tgctgacaga
    12001 tcagaacatt ataacaaaat aattattcca aatttaaaca agcacaaaat agttatttct
    12061 gatagaaatt gaccttctac tattgcgtat caaggttttg gtagaggtat ttctttacgt
    12121 tttctggatt atcttagaaa agaagcgaca tatggtataa aaccagattt gattattttt
    12181 ttagatatta ttccttcgga ttatattaag gtttgtaagc aaagacagga aacaagacat
    12241 aaaaaattga ctcgttttga acaagaaaaa cttgattttc acaaaaaagt aagaagaggg
    12301 tttttatatt tagcaaacaa agatacaaaa cactgaaaaa taattaatgc caatcagcca
    12361 gtagataaag tgtttcaaga tatacaaaag attgtagatt atactcttaa acatatttaa
    12421 tattccaaaa tggttaaaat agagcatata aatattaatg ggcaatctta tattccttgt
    12481 attgggttgg aagttcatat tgagttgaaa actaatacca aaatgttttg tagttgtaaa
    12541 aatgattcta atgaaattat accaaacact aatgtttgcc cgatttgtat ggggcatcct
    12601 ggcacattac cagtacctaa taaagatgct atagagaaaa ttattaagct tggtatggca
    12661 ttgggttgtg atatacaaaa agaagcaaca tttgatcgca agaattattt ttatcctgat
    12721 ttgccaaagg ggtatcaaat tagtcagtct tttgtgcctt tttgtttagg aggtaagatg
    12781 cctttgtttt tgtcattaaa tgatgaagta aattatgtac aattgcgaga aatacattta
    12841 gaagaagatg cgggtaaatt gttacataca aatgcgccag gtattgccct tgtggatttt
    12901 aatagggctg gagtgccatt ggttgaatta gtaacagaac cagttataca ttctggcgaa
    12961 gaagtacgat tatttggtga aagtttgcaa ttacttgtaa attatttgga tatttccaat
    13021 gctgatatgg aaaaaggaga aatgagagta gaagttaata taagtatatc aaatgttgag
    13081 caaaatgata taaataatac attaataaat ttaggacaaa aggtagaaat aaaaaatatt
    13141 aattctttta gtgcagcttg caaggcagta gaatttgaaa tatctcgaca aacagaaata
    13201 ttacaaaatg gacaaaaagt agtaggggaa actcgtggtt gagatgatat caaacaaatt
    13261 actttttctc aaagattaaa agaagagtca aatgattata gatattttcc tgagccagat
    13321 ttggtaccaa tagaaacaca aagtttttta tatcaagaac taccagaatt accttgggtt
    13381 agacgtcaac gatttataca aaattataag ttggaaccaa aaaaggtttt gctttttatc
    13441 aaaaatatag agttgggaga tttttttgaa gtgttggtca aaaccagtat agagtcgata
    13501 aataatattg agcaaaacca aaatattata gaattgtgtt ataattattt aacagttgat
    13561 tttttaggct tgattgctaa agaaaacaag acattttcaa aagatttatt aaatccaaac
    13621 aattttgctt tattaattaa atatttaata gaaaataaaa tttcatctaa aggcgccaaa
    13681 attattattg agaatttagt acgagatcct aatttgaaca ctgaagatat attaaataat
    13741 aataatttat tacaagtatc tgatgaaaat tttgtagtag agttggtaca aaaagtttta
    13801 ctagataatc caagctctgt acaagaatat aaaaatggta aggagtcagt gttacaattt
    13861 ctgataggtc aagcaatgaa agcaagtaag ggcacagcaa atccacaaat agttaaagaa
    13921 attatgttaa agcttcttgg gtagtattgt aattagatat ttatttgttt ttggttttat
    13981 tttatt
//
LOCUS       DAOTJA010000010        13951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_4820_length_13951_cov_6.714090, whole genome shotgun sequence.
ACCESSION   DAOTJA010000010 DAOTJA010000000
VERSION     DAOTJA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4820_length_13951_cov_6.714090"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..275)
                     /locus_tag="P5241_01240"
     tRNA            complement(202..275)
                     /locus_tag="P5241_01240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Gln,seq:ttg)
     gene            complement(369..602)
                     /locus_tag="P5241_01245"
     CDS             complement(369..602)
                     /locus_tag="P5241_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32193.1"
                     /translation="MEGILYGAIASVVSFILYIPIIFIFGSKIDKFLGFVSITKYFVS
                     HMLLIFLAQLVFGVLLGVVSSILATNKYLKQVE"
     gene            complement(624..1295)
                     /locus_tag="P5241_01250"
     CDS             complement(624..1295)
                     /locus_tag="P5241_01250"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRV32194.1"
                     /translation="MEISFSTNVKRIFKSGYDNFKRNSWLSIATIITMVLSVMLLTGT
                     LLLNYGSNAFVKEIQNKVDVSIYFKTDVSEVDILKIQEELTKLDTVAKVEYTSREKAL
                     QDFKEQSAKDQIIIQTLEEIGDNPLSASLNIKAKKPGDYTPIISYIENSLFKDKLLSV
                     DFTENKLLVDRANSLSFGLKTSTIILTVILSSVAIVVAFNTIRMAIHSVGEELKIMNL
                     VGGSN"
     gene            complement(1423..2106)
                     /locus_tag="P5241_01255"
                     /pseudo
     CDS             complement(1423..2106)
                     /locus_tag="P5241_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2136..2282)
                     /locus_tag="P5241_01260"
     CDS             complement(2136..2282)
                     /locus_tag="P5241_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32195.1"
                     /translation="MNLNIIDVQDVLKDDTMDETNIETKKGKIDLLGKMGDIVKHILD
                     CCRE"
     gene            complement(2286..2666)
                     /locus_tag="P5241_01265"
     CDS             complement(2286..2666)
                     /locus_tag="P5241_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32196.1"
                     /translation="MSILNSDELLKKLNDLKKDNKLDLSSDEDLSLAIMNLIAIEEHL
                     FFTYSKTNKTVYLDMLNESRDIRKTLMSKIVKNKDGEVWCISKHLLSASMRLMEVGTK
                     MLNNGEKEKAEDLFQKSYKLYSMF"
     gene            complement(3326..3706)
                     /locus_tag="P5241_01270"
     CDS             complement(3326..3706)
                     /locus_tag="P5241_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32197.1"
                     /translation="MAIYEDKIKKTKSQTTIPICLISDLRYFKHLLESGKINDYDYDN
                     KRIKNLLWDFFYQEVNPYKQVNTHLKVKDISSKQTPEEIKKNIQKIAKQKKQLALDQY
                     LDPYQRYLKRISKDKNKLIQEMKW"
     gene            complement(3926..4042)
                     /locus_tag="P5241_01275"
     CDS             complement(3926..4042)
                     /locus_tag="P5241_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRV32198.1"
                     /translation="MSLRGDGTIDVSLIAKKYGGGGHYNAAGFTFKDSDDIP"
     gene            complement(4067..4309)
                     /locus_tag="P5241_01280"
     CDS             complement(4067..4309)
                     /locus_tag="P5241_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32199.1"
                     /translation="MTFDNWNKIAKDLEDKSKRQIIINNGHTILMYMQSIVKRLIKTA
                     DKVMFENEISLAVNSPILNSEIGHELTKNGIHIGII"
     gene            complement(4364..4693)
                     /locus_tag="P5241_01285"
     CDS             complement(4364..4693)
                     /locus_tag="P5241_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32200.1"
                     /translation="MAYKKFGNEAFYYGVSCQPNISNDLLLLLQDKEIYFIDVCFAKD
                     IMEQILSNNRKVIIIDHHISQEDIVKLGYEYMFDVSHCASVLAFKYFFPHSKMPKILK
                     YVEDGDL"
     gene            complement(4843..5292)
                     /locus_tag="P5241_01290"
     CDS             complement(4843..5292)
                     /locus_tag="P5241_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32201.1"
                     /translation="MSIKQNNDIKTIANITNITVPFINKENTSTIQTGSLDTSSTTTN
                     SEGFVDCTNTDSDINSNADFFKCFLEQFKVCGASVQKAVGSRGESIVFKIYGLEQNKC
                     HYRVDIDGHGGDCLFELKDMADGKLFYQLLGSDQGLQFVVDTYCVSF"
     gene            complement(5858..6718)
                     /locus_tag="P5241_01295"
     CDS             complement(5858..6718)
                     /locus_tag="P5241_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRV32202.1"
                     /translation="MAPYINSKLTPFLTNDFVRPIIGQVKTTINFREIMDKNKIFIVN
                     LSKGYMDEFSSYFIGMIIIGKFLLAAFSRAELSEAERVDFNLYADEFQNFTTKNIPTI
                     LSEARKYHLILTLAHQYIAQLEEKIRESAFGNVGTLVSFRVSAQDAEYLAKYMVPVFS
                     ASDLMNIANYNAYIKLMIDGHPARPFNIQTIKPEAPEQSYKTEIKTLSRQRYCRPRLE
                     VENEIQSRYRIAREDEDEDEDSNTEDNDDFLSFLDTNNSLDVNNDDNNDKSDKDKSQS
                     DDRIERKDNN"
     gene            complement(6764..7354)
                     /locus_tag="P5241_01300"
     CDS             complement(6764..7354)
                     /locus_tag="P5241_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRV32203.1"
                     /translation="MLSRGSSSPADLPSEGTVLGLSKFRGEVKKVMIKNKDRRLHMYI
                     IGQTGTGKTTFLKNMMIQDIQQGKGLCFLDPHGDEAEDLLNYIPKERIQDVIYFNPAD
                     RDMVMGLNMLEYTANDSKGPTMIANELIEIFDKLYDLKQTGGPIFEQYMRNALNLLMS
                     DPDQQYTLADVSRVLTDPLFRRYLLGRTNDLLTKDF"
     gene            complement(7490..8305)
                     /locus_tag="P5241_01305"
     CDS             complement(7490..8305)
                     /locus_tag="P5241_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32204.1"
                     /translation="MHYKLLSIRVPKEINLDSKVEDQRKIAEIISASEQIIASLAQYK
                     NPIVFEIASPIGSNEIYFYVSCLKKEQEFVKKIITAFYPFAEVEEVDDYSIFAPKNYT
                     AASYLHLEKSYSLPLKTYQHFENDPLNAIVNAFSHIDQGEGAAMQVIIKPAGGYAKKK
                     IMKDLQDARKGKSYNDLFGKIAIVDTNLLLKTINPETEEEKKFKEIKPVDETLVKNLE
                     FKVSSPLFETNIRLLVSSHDKYHANFVLSHLESGFSEINMPSMNGFKIKRVSS"
     gene            complement(8439..8852)
                     /locus_tag="P5241_01310"
     CDS             complement(8439..8852)
                     /locus_tag="P5241_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32205.1"
                     /translation="MEQINFKGVKQIEKYIEDKHQIDKKDLSKSITKEMIKEAIESII
                     ETELSKLPTKTESISQAISLEVPVASAPNMQHQAIQFDSKGKKQAEIMLENLVDIALT
                     KSVSQAIKTSLKTKNAYLIDKLHDILIDKFYEILK"
     gene            complement(9096..9827)
                     /locus_tag="P5241_01315"
     CDS             complement(9096..9827)
                     /locus_tag="P5241_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32206.1"
                     /translation="MFLVTNINVYNNKEIPSGVLLPRIKQTVLDSNNKLYWTLVSLIH
                     SDSNVYLANLLLLNNKDITKQIELMFPQINKAEVTKNYYTHTLLVNIEEKHPVLAVKI
                     NNVDINNLYIDQNGILFKADTVDKSKLPEIIFFDFVDENLLNKSVVHREIFSKSQMDT
                     ILTLLNYRENFQLGINHFEYTFRKPNSLVLQTKFNFMIYLSLRPDIVDALKAAEVYYL
                     KVIKPKNKNIEYIDVRYYPEKLFYK"
     gene            complement(9934..10230)
                     /locus_tag="P5241_01320"
     CDS             complement(9934..10230)
                     /locus_tag="P5241_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32207.1"
                     /translation="MPGQNDGKGEYRETHSNSNCTDFQTRRLNIKYKNIKGDSQFAYM
                     LNGTFIALGRIMIAIIENYQTKTGSIKIPTVLWPYMNGIKEIGNKHGKTKFKLF"
     gene            complement(10231..11232)
                     /locus_tag="P5241_01325"
     CDS             complement(10231..11232)
                     /locus_tag="P5241_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRV32208.1"
                     /translation="MLDIKKIHKNPEWFKEKLALRDIDSDIIDRLLQVDDNRKQCIQK
                     IDDLKFKRRNLEKKFVQDNSGQSLEVLKEVKRAIDILEKDLDKYTKEFDDLMYSLPNV
                     PLADVPEGEKDKIIVEKGKKPLFTFIPKDYLSISEQLDLINIEDASNVSGSRFSFIKN
                     ELVFLQFALIKYVYDKFCQKYNFIPILPPFFIKSEYFKAMGYIDIEADKNETYYLPDD
                     DLYLIGTAEHILGAMNANKIFQEEQLPQRFLGFSSCFRREAGSYGKDTKGILRSHQFD
                     KIEMLSICTESQSLKEQDLFIDLETKLMEDLVLPYRIVQTAVKNLSRPSARSFDIEC"
     gene            complement(11267..12175)
                     /locus_tag="P5241_01330"
                     /pseudo
     CDS             complement(11267..12175)
                     /locus_tag="P5241_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12296..12721)
                     /locus_tag="P5241_01335"
     CDS             complement(12296..12721)
                     /locus_tag="P5241_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRV32209.1"
                     /translation="MASLQDIIKERIKKIEGLRKLGIDPYPEKTDKDHNIKNILDGFD
                     VLVANNTKVCVAGRIMSIRGHGAILFAHIQDETGKIQFLLKQDQIGQEKMDLFEHYFD
                     LGDFIEIKGVVFQTNTGEKTILVDDFKILCKALRPIPTE"
     gene            complement(13006..13092)
                     /locus_tag="P5241_01340"
     tRNA            complement(13006..13092)
                     /locus_tag="P5241_01340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13056..13058),aa:Ser,seq:gga)
     gene            13498..13893
                     /locus_tag="P5241_01345"
     CDS             13498..13893
                     /locus_tag="P5241_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV32210.1"
                     /translation="MKGYVTNIEKDSLENNNFRKVLYTAPHCQLVLMSLLPKEEIGEE
                     VHTLDQFIRIEQGQGLAVLNDIEYPIEDGSAVVIPEGTKHNIINTSETESMKLYTLYA
                     PPEHRDQVIHQTKNDALQDNEEFDGKTTE"
BASE COUNT         4882 a         2433 c         1454 g         5182 t
ORIGIN      
        1 tgcttctatc aatgtatttg ttaatttatt gtaattatat cataatatat gggggtttgc
       61 aactattcag cagaacttgt agtaattagt gatttagaat tgtaaattag aaaaatacaa
      121 agacaatggg ataaaaaata gtttcttaat ctgtataaat ctgcatattg atcttagtat
      181 atttatccaa ataaagtctt gtggctgcgg gacctggatt cggaccagga taacgaactt
      241 caaaggctcg cgtcctacca ttagacgatc ccgcaaaacc aatacaaaaa aatattatca
      301 tattttctta tattctgcaa atatcttata atattaaagg ggtctaaacc ccgtaaatat
      361 atattttatt attctacttg ttttaaatat ttattagttg ccaatatact agaaacaaca
      421 cccaacaaaa caccaaacac taattgcgcc aaaaaaataa gcaacatatg agaaacaaaa
      481 tatttagtaa tacttacaaa acctaaaaat ttatctattt tgctaccaaa aataaatata
      541 attggaatat acaaaataaa tgaaacaaca ctggcaattg ctccatatag tataccttcc
      601 actataaaag gtccacgaat aaatcaattc gaacctccca ccaaattcat aatttttaat
      661 tcttctccta cactatgaat agccatacga attgtattaa aggccaccac aatagcaacg
      721 ctacttaaaa taacagttaa tattattgtg ctagttttta aaccaaaaga caaactattt
      781 gccctatcta ctaacaattt attttctgta aaatcaacac ttaataattt atctttgaac
      841 aaagaattct ctatataact aataatcggc gtgtaatctc cgggtttttt agctttgata
      901 ttcaaagaag cagacaaagg attatcgcct atttcctcga gagtttgtat aataatttga
      961 tctttagctg attgttcttt aaaatcttgc aaggcttttt cccgagaagt atattctact
     1021 ttggcaactg tatccaattt agttagttcc tcctgtattt tcaaaatatc tacttctgat
     1081 acatctgtct taaaataaat actaacatct actttgttct gaatttcttt tacaaaagca
     1141 tttgacccat aattcaaaag caatgtacca gtcaaaagca ttacactcaa aaccatcgta
     1201 ataattgtcg ctattgacaa ccagctattt cttttaaaat tatcatagcc agatttaaaa
     1261 attcttttta catttgtaga aaatgaaatt tccataacat aataattaaa aaactattaa
     1321 atttttattt aacatttttc aatttccatt tgtttattat tctattgata attcgtttaa
     1381 tgaaaattgc taaatattaa ttgtcagatg tttagccctt ggttaaagaa tatatttacc
     1441 attttgttca tcgcgtacaa tacaaccatt ttccaaagtt attaccctcc gacccaaagt
     1501 attaacaatc tctttttcgt gagtagcaaa cacaactgtt gtacccaatt cattaatttt
     1561 aatcaaaagc ctaacaatat ctcacttatt aataggatcc aaattaccag tgggttcatc
     1621 agcaagcaat aaatctggtt tctgaataat agcacgtgct atagccactc tttgtttttc
     1681 tccagctgac aattgatagg gaaaattatt tgccttatct ttaagtccaa ccaaatttaa
     1741 aacctcaggc acttcttctt gaatttcttc atcactcaaa ccaatagact ccaaaacaaa
     1801 agctacattt tcaaaagctg ttttatgact caaaagttta aattcttgaa ataccactcc
     1861 tatttttctt ctcaacatag ttaatcccat acaattcatt ttagtaacat ctttccctga
     1921 aaaaataatt ctacctttgg ttggtttttc ttctccaata attagtttta acaaagtagt
     1981 tttgcctact cctgattttc ccaccaaaga cacaaactca ttattatcta tttcaaaaga
     2041 aacattgtcc aaaattaatg tttcgttata tattttagta acatttttac aatataataa
     2101 agacataatc aacaaattta aaattatttt ctaatttatt ctcgacaaca atccaaaata
     2161 tgtttaacaa tatcccccat tttacccaat aaatcaattt tccctttttt ggtttctata
     2221 tttgtttcat ccattgtgtc atcttttaat acgtcttgaa catcaataat atttaaattc
     2281 aaacctcaaa acatagaata caatttataa gatttttgaa acaaatcttc tgctttctct
     2341 ttttctccat tatttaacat tttggtgcca acttccatta aacgcattga agcagacaat
     2401 agatgttttg aaatacacca tacttcacca tctttatttt ttacaatttt tgacattaaa
     2461 gtttttctaa tatctcgcga ttcatttaac atatccaaat aaacagtttt gtttgttttg
     2521 ctataagtaa aaaataaatg ttcttctatg gcaattaaat tcataatagc caaagataga
     2581 tcctcgtcag aagacaagtc taatttatta tcttttttca aatcatttaa ttttttaagt
     2641 aattcatcag aatttaatat gctcatatta ttagtttatt atacagttta ttatactaat
     2701 aacgacaagg tatgtattac atatttattt tattattagc agagtataaa atttattata
     2761 atactagcaa tagttaacaa caatataggt ataactactt tttgataata aaataaaacc
     2821 ttaccattat tatattgctt aattagttta tccaaccata ttcccaatga taacaaaatc
     2881 atacctatta tactactaaa caacaattta tcaaaaaatc agatcttatt gtgtatatta
     2941 ccagtaattc ctaacaattt taagggtata aaagtaatag tataataaat tatcaaccaa
     3001 acgcccttac gaacaaaact ataagaactt tgtttataaa atttaaattt attagccaat
     3061 caattgtcag ttatcacaga taaagctaac aagaaagcac caatccataa tccagttacc
     3121 aaatcatcaa tacccaaata acgacatagc cccacacccg ctcctatagc tactgtacaa
     3181 actggacact gcgctcaaac aatataaggc acaaaatatc cgattagcaa aatggttata
     3241 atcaaaagta ttttatgtat aacattcatt attatttttt ttctaattct ttttttataa
     3301 tctctgacct attaaaaaaa tctaatcacc atttcatttc ttgtatcaat ttatttttgt
     3361 ctttagaaat acgttttaga tatcgctgat atggatctaa atattgatcc aaagccaatt
     3421 gctttttctg tttagcaatt ttttgtatat tctttttaat ttcttctgga gtttgtttac
     3481 tcgatatatc ttttactttc aaatgcgtat taacttgttt atatggattt acttcttggt
     3541 aaaaaaagtc ccaaagtaaa ttttttatac gtttattatc ataatcataa tcattaattt
     3601 taccactttc taacagatgt ttaaaatacc gtagatcaga tatcaaacaa ataggaatag
     3661 ttgtttggct ttttgttttt ttgattttat cttcatatat tgccaaagat caattgtcta
     3721 aaacatcttt ttgatttcaa gaaggctttt tctttttaaa tttttcttta taatattgct
     3781 ctttacaata ttcctcttct tgttcttcca tttttgctat aaattgttca taatcttcca
     3841 taattaaaat aaaattgtat ttttattaaa ccaaagggta tatatattta tactatcaca
     3901 aaataaatta tttttcaatc aaatttcaag ggatatcatc actatcttta aaagtaaacc
     3961 cagctgcatt ataatgtcca ccaccaccat atttttttgc tattaaagat acatcaatag
     4021 taccatcacc tcgtaaactc actcaaattg tatgtcctgt ttgacttcaa attattccta
     4081 tatgaattcc attttttgtc aattcgtgtc caatttctga atttaaaatt ggagaattta
     4141 ctgccaatga tatttcattt tcaaacatta ccttatcagc tgttttaatt aatcgtttta
     4201 caatagactg catatacatc aatatagtat gcccattatt aataattatc tgccttttgc
     4261 ttttatcttc taaatccttg gctattttat tccaattatc aaaagtcatt tcttgtattg
     4321 ctaaatatgc taatatctcg tgagaatttt tgtatgttca ttttcataaa tcaccatctt
     4381 ctacatattt taatatcttt ggcattttgc tatgtggaaa aaaatattta aaagccaaaa
     4441 ctgacgcaca atgtgataca tcaaacatat attcataacc cagcttgact atatcttctt
     4501 gagaaatatg atgatcaata attataactt ttctattatt gcttagtatt tgctccataa
     4561 tatccttagc aaaacaaaca tcaataaaat atatttcttt atcttgcaac aataataaca
     4621 aatcattaga tatattcggt tggcaagata ctccgtaata aaaagcctca ttgccaaatt
     4681 ttttatatgc cactcaggcg ccactaaatc cgtccaaaca attaccatga tatattacta
     4741 ctttatcttg aacaatattt ctaaacattt taaattttta gtcatatatt tattattaat
     4801 catatatcta tcaaatgttc caatataact tctaaattat ttttaaaatg atacacaata
     4861 tgtatccaca acaaattgta atccttgatc agatcctaat aattgataaa ataatttacc
     4921 atctgccata tcttttagct caaacaaaca gtctccgcca tggccatcta tatctactct
     4981 ataatgacat ttattttgct ccaaaccata aattttaaac acaatagatt ccccccttga
     5041 acctactgct ttctgaacag atgcaccaca tactttaaat tgttctaaaa aacacttaaa
     5101 aaaatcagca ttggaattta tatctgaatc tgtattagtg caatctacaa aaccctcgct
     5161 attggttgtg gtagatgatg tatccaaaga tcctgtttga atggttgaag tattttcttt
     5221 gtttataaat ggtacagtaa tatttgtaat attggctatt gtttttatgt cattattttg
     5281 ttttatactc ataaacatca ttaatccacc acagatcaaa agcccaattg tcaataaaat
     5341 tttaaatcaa ttattcataa tattaatttt tattatttat aaattaaatt tgagttttaa
     5401 aaactcattt tttagacata aaatatctta tcaatattgc taccaatgcc ccaataaatg
     5461 ttgctatatt tgctcataaa tatctattca atgcaaatat attatacggt aacaaaaagg
     5521 ccgttattat attgtttata cacagagtca acatactaac aataacaaat tgtacaaact
     5581 gaatattgcg tttttctatt gccaaacttt taaatgtcca acctttattt aacataaaac
     5641 taaccaatac tccacataaa aaagtaaaag atcttatacc aataaatgtt cagccagagg
     5701 taatacccgt aacaaacata aaaatattca ataacccaaa atcaaccaat gtattaagca
     5761 ctccgacaac actaaaacga ataaactcaa aatgtaaaaa atagtttata aaatttatga
     5821 atttgctcca gattttatgt atcattgtat tactttatta gttattgtct tttctttcta
     5881 ttctatcatc tgattgtgat ttgtctttat cacttttgtc attattatca tcattattta
     5941 catctaaaga attattagtg tctaaaaatg agagaaaatc atcgttatct tcggtattgg
     6001 aatcttcatc ttcatcctca tcctctctag caattctata tcttgattgt atttcatttt
     6061 ccacttccaa tctcggacga caatatcttt gtcttgacaa tgtttttatc tctgttttat
     6121 aagattgctc tggggcttct ggtttaatag tttgaatatt aaaaggtctt gctggatgtc
     6181 catcaatcat aagtttaata tacgcattat aattagcaat attcatcaaa tcactggcac
     6241 taaatactgg gaccatatat tttgctaaat attctgcatc ttgagcactt actctaaatg
     6301 aaacaagcgt accaacatta ccaaaagctg actcacgaat tttctcctcc aattgggcaa
     6361 tatattggtg cgccaaagtc aaaattaaat gatattttcg agcttctgat aaaattgttg
     6421 gaatattttt agtagtaaaa ttctgaaact catcagcgta taaattaaaa tctactcttt
     6481 ctgcttcgct caactcagct cttgaaaaag ctgctaacaa aaatttacca ataataatca
     6541 tacctataaa ataactacta aattcatcca tatatccttt ggataaattt actataaata
     6601 ttttattttt atccattatc tctcgaaaat ttattgtagt ttttacttgg ccaataattg
     6661 gacgcacaaa atcattagtc aaaaatggag ttaatttaga attaatataa ggcgccatat
     6721 tagctagcga taaatcacca cccgcttttt ctgcttcttg ttctcaaaaa tctttagtca
     6781 ataaatcatt tgttcgaccc aacaaatatc tcctaaacaa tggatcagtc aaaactcgag
     6841 aaacatcagc caaagtatat tgttgatcag gatcagacat caataaattc aaagcattac
     6901 gcatatattg ttcaaaaatt ggaccacctg tttgtttcaa atcatataat ttatcaaaaa
     6961 tttcaatcaa ttcgttagca atcattgttg gtcctttact atcattagct gtatattcta
     7021 acatattcaa acccattacc atatctctat cagcaggatt aaaataaata acatcttgaa
     7081 ttctttcttt aggaatatag tttaatagat cttcggcttc gtcaccatgg ggatccagaa
     7141 aacacaaccc cttaccttgt tgaatatctt gaatcatcat atttttcaaa aatgtagttt
     7201 taccagtacc tgtttgccca ataatataca tatgtaacct tctatctttg ttttttatca
     7261 ttaccttttt tacttcacct ctaaatttgc ttaatcccaa aaccgttcct tcgcttggca
     7321 aatcggcagg gcttgacgat cctctgctca acaatcattt tatattaggc acttctaatt
     7381 tactatgagg caaatgtcaa attgtattta tctctgatgt attcaaaact atcgcatttt
     7441 taacatcaaa tattctataa acatatttga aaataaaatt cgttaatcct catgacgaaa
     7501 ctctttttat tttaaagcca ttcatactcg gcatattaat ttccgaaaac ccactttcca
     7561 aatgtgataa aacaaaatta gcatgatatt tatcatgaga agataccagc aatcttatat
     7621 tagtttcaaa caaagggcta gaaactttaa actctaaatt ttttaccaaa gtctcgtcta
     7681 ctggcttgat ctctttaaat tttttttctt cttctgtttc aggattaata gtttttaata
     7741 acagatttgt atctactatt gctattttac caaacaaatc attataactt ttacccttgc
     7801 gagcatcttg taaatctttc ataattttct tttttgcata tccccctgct ggctttataa
     7861 taacttgcat tgcagcccct tctccttgat ctatatgact aaaagcattg acaatagcat
     7921 ttaagggatc attttcaaaa tgttgataag tttttaatgg taaagaataa gatttttcta
     7981 aatgtagata agaagcagct gtatagtttt tgggcgcaaa aatagaataa tcatcaactt
     8041 cctccacttc ggcaaaggga taaaacgcag taataatttt tttcacaaac tcctgctctt
     8101 tttttaaaca agatacataa aaataaattt cattactacc aataggagaa gcgatctcaa
     8161 aaactatcgg attcttatat tgtgctaaac tcgcaattat ttgctcgctt gcactaatta
     8221 tttcagctat ttttctttgg tcttctactt tgctatctaa atttatttct tttggtactc
     8281 taatagacaa caatttataa tgcaaagatt tattaaccct ctttttatac gcaatataat
     8341 taaaaaatca tataaaacaa acgagtatcg ccagaatact tatgctataa attagtattg
     8401 tattaaaaat atttaaagat tgcatattaa ctacttaatt attttagaat ttcataaaac
     8461 ttatctatca aaatatcatg cagtttatct atcaaataag cgtttttggt ctttaaactt
     8521 gtttttattg cttgcgatac acttttggtc aaagcaatat ctaccaagtt ttctaacatt
     8581 atttctgctt gttttttccc tttactatca aactgaatag cttggtgttg catattgggc
     8641 gcgcttgcta ctggaacctc caaagatata gcttgtgata tactttctgt ttttgtgggc
     8701 agtttactca gttctgtttc tataatactc tcaatagctt cttttatcat ttctttggta
     8761 atagatttgg acaaatcttt tttgtcaatt tggtgtttat cttctatata tttttcaatt
     8821 tgtttaaccc ctttaaaatt tatttgttcc actatataaa tttaataaaa ttattaaaat
     8881 gattaaatta ttaagtagat atattctata atataattat atcatactta taataattta
     8941 tcaacactcg cctaccaata atttttatca aaatgttcca atatatacaa aatattataa
     9001 taaatactac ataataaaag attaagatta agattaagat taaaaattaa acatatactt
     9061 aatagtgctt cataatcaca aaattttatc aataattact tataaaacaa tttctctgga
     9121 taatacctaa catctatata ttctatattt ttatttttgg gtttaataac ttttaaataa
     9181 tacacttctg ctgcctttaa ggcgtccaca atatctgggc gcaaagataa atatatcata
     9241 aagttgaatt ttgtttgcag taccagagaa ttaggctttc taaaagtata ttcaaaatga
     9301 ttaatcccta attgaaaatt ttctcgataa ttcaacaaag ttaatatagt atccatttgt
     9361 gatttagaaa atatttctct atgaacaaca gacttattta ataagttttc atctacaaaa
     9421 tcaaaaaata taatttctgg taatttagat ttatcaacag tatcagcctt aaacaaaata
     9481 ccattttgat ctatgtataa attatttata tctacattat taattttaac agccaacaca
     9541 ggatgttttt cttcaatatt gaccaacaag gtatgagtat aataattttt tgtaacttct
     9601 gctttattaa tttgaggaaa cattagctct atctgtttgg ttatatcttt attattgagt
     9661 aataataaat ttgccaaata cacattgcta tctgaatgta tcaaagaaac taatgtccaa
     9721 tataatttat tattactatc cagtactgtt tgtttaatac gtggcaacaa aaccccactg
     9781 ggtatttctt tattgttata tacatttata ttggtcacca aaaacatttt ggaaaaaaat
     9841 atataataca aacaaactat aattaaaaca ctacaaaaaa atcataataa ttttgtagtc
     9901 cttctacgcc tttttaggtt tttgagtctt gggctagaat agcttgaatt ttgtttttcc
     9961 atgtttattg cctatctctt taataccatt catataaggc cataaaaccg taggaatttt
    10021 tattgaacct gtttttgttt gataattttc aattatagct atcataattc taccaagcgc
    10081 tataaaagta ccattaagca tatatgcaaa ttggctatcg cctttaatat ttttatactt
    10141 tatatttaat cttcttgttt gaaaatccgt gcaattagaa ttagaatgag tttctcgata
    10201 ttctcccttg ccatcatttt gtccaggcat tcaacattct atatcaaaag aacgagccga
    10261 tggacgagat aaatttttaa cagccgtttg aactattcta taaggcaaaa ccaaatcttc
    10321 cattaatttg gtctctaaat caataaataa atcttgttct ttcaaagatt gactttcagt
    10381 acaaatactc aacatttcta ttttatcaaa ctgatgcgaa cgcagaattc ctttggtatc
    10441 tttgccataa gaccctgctt ctctcctaaa acaagacgaa aaacccaaaa atctctgtgg
    10501 caattgttcc tcttgaaaaa tcttattagc attcattgcc cccaaaatgt gttcagcagt
    10561 accaattaaa tacaaatcat catctggcaa ataatatgtt tcgtttttat cagcttcaat
    10621 atcaatatat cccatcgctt taaaatattc tgatttaata aaaaatggtg gcaaaattgg
    10681 aataaaatta tatttttgac aaaatttatc atatacatat ttaatcaaag caaattgtaa
    10741 aaataccaat tcatttttaa taaaactaaa acgactgcca gatacattac tagcatcttc
    10801 tatatttata agatctaact gttcagatat acttaaataa tcttttggta taaatgtgaa
    10861 caagggcttt ttaccttttt ctacaataat tttatctttt tcaccttctg gtacgtcagc
    10921 caatggtacg tttggtaaac tatacattaa atcgtcaaat tcttttgtat atttatccaa
    10981 atccttttct aatatatcta tagccctttt tacttctttt aatacctcca aactttgtcc
    11041 agaattatct tgtacaaact ttttttctaa atttcttctt ttaaatttta aatcatcaat
    11101 cttttggata cactgttttc tattatcatc aacctgtaat aatctatcaa tgatatctga
    11161 atcaatatct cgcaaagcca acttttcttt aaaccactca ggatttttat gaattttttt
    11221 aatatctaac ataataagta tttattaaat ttctaaatta taatttttaa atatttttgg
    11281 gattttgtct tacaaacgga aaaaacaaaa cttcttttat tgaatttact cccagcaata
    11341 cactaatcaa cctatctata cctatgccta aaccaccagc aggtggcata ccatattcta
    11401 aagcctctac aaaatcaaga tccaaaggat gtgcctcttt atcgcctttt tgtaacattt
    11461 tagattgttc ttcaaacctt cttttttgct ccatagcatc atttaattca gaaaaaccat
    11521 tacaaacttc cttgccattc attatcaaat aaaacctcaa aacatgctgc aaatcatctg
    11581 gtttttgctt agccaaagga gataattcca atggctggtc aataacaaat gttggatttt
    11641 ttatttctcc tctaaattgt ttaaatatta aatctgctaa ttttgcccta gatgattgtt
    11701 gttcaaactc aatattattt ttttgcaaat attcttgaaa cttatccaaa gaatcactat
    11761 cgcaatccaa accagtcttg gtcttaataa aatccctaaa tactattttg gggaaaggca
    11821 cttgaatatt gcctcagata gattgttctt gaagtttact attcaaaaaa ataattaatt
    11881 tttcgagcat gtccataagt tcatctcttg aagcaaaggc tcaatataat tctaaattta
    11941 taaattctgg gttgtgatct ttgtctatac cttcgttgcg aaaacatttt gccatttcaa
    12001 aaattttctc ataaccacct ataagcaatc tttttaaata caattctgga gcaatcctta
    12061 aatataaatc catattcaaa gcattcaaat gagtcttaaa gggttctgct aatgctcccc
    12121 catatacagt ttgcaaaatc ggagtttcta cttctttaaa atcaagatta tacaaaaatt
    12181 cacgaattca ttttgttaaa tcaatacgca aatcaaaaat ttttttagtt tgtttatcta
    12241 aaatcaaatc aagatatctt tttcttattt tttcttcttc gtcctctaaa ccaaatcatt
    12301 cagttggtat aggtcttaaa gccttacaca atattttaaa atcatccacc aaaattgttt
    12361 tctcaccagt atttgtttga aacaccacac ctttaatttc tataaaatca ccaagatcaa
    12421 aataatgttc aaataaatcc attttttctt gtcctatttg atcttgtttc aacaaaaatt
    12481 gtattttacc agtttcgtct tgaatatgag caaataaaat tgcgccatgc cctcgaatac
    12541 tcataatcct tccggctaca caaactttgg tattattagc aaccaagaca tcaaaaccat
    12601 ctaatatatt ttttatatta tgatctttat ctgttttttc tggatatgga tcgatcccta
    12661 attttctcaa tccttctatt tttttaattc tttctttaat aatatcttgt aaactcgcca
    12721 ttatttcaaa ttattaaaag ttttaaattt attaatatta tatattaaat atatcaaata
    12781 atcgaacaaa gtgcaagctc aatattccaa gaataatcaa agctattata gtatttcctg
    12841 ccctgacaac ttcacagact aagtaattct ttaaaaatat cagagaaaca accaaaacta
    12901 tcacaacatt aacaaaaata taaataacca aatctatcac aatatattat aatatctcaa
    12961 taatctccaa tggctttttg tatgtacaat tcattccaca taatgcggtg agggtgggat
    13021 ttgaacccac ggtacagttt cccgtacact tgctttccaa gcaagcactt taagccactc
    13081 agccacctca ccatattaaa caagatcatt tattgacatt cgagctttaa tacaataacc
    13141 tacaataatc ctatttctcc caatcattat acaatacttg cttaaaatat atagatatct
    13201 aatcatttaa attctatcat aaatttatat ttatttcaat cttttccatc ataacttact
    13261 gacatttaca tattcttggg aatatgtaaa tgtcagtaag ttttcttaat tttagatatt
    13321 ataaaagaat tttgaacgtc ttaaaatgaa acaagttgtt taaaatgatt tattgctaac
    13381 atattgtgat actctatata gtattgacac aacaatttgt tttaacctat tgaataattt
    13441 tattatttat gctattatta atatcaatac atttaataaa ctcaataaac tcaaaatatg
    13501 aaaggatatg taactaatat tgaaaaagat tctttagaaa ataacaattt tcgaaaagtt
    13561 ctttatacag ccccccactg ccaattagtc ttgatgtcat tattaccaaa agaagaaatc
    13621 ggcgaagaag tacacacctt agatcaattt attcgtattg aacaaggaca gggtttggca
    13681 gttttaaatg atatagaata tccaatagaa gatggctcgg ccgtagtcat tcctgaaggt
    13741 accaaacata atatcataaa cacttcagaa acagaatcaa tgaaactcta tactctttac
    13801 gctccaccag aacaccgcga ccaagtaatt catcaaacta aaaatgatgc tctacaagat
    13861 aatgaagaat ttgatggcaa aacaactgaa taatattatt gtccaaaaat aatcaaacaa
    13921 aaacctaaat actaatatcc agcattacca t
//
LOCUS       DAOTJA010000011        12928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_5270_length_12928_cov_6.062612, whole genome shotgun sequence.
ACCESSION   DAOTJA010000011 DAOTJA010000000
VERSION     DAOTJA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_12928_cov_6.062612"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="P5241_01350"
     CDS             <1..1258
                     /locus_tag="P5241_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HRV32211.1"
                     /translation="SITRTSKNTMNFEDIQINDKITVTGELIKEASKDVRGEIIAKLI
                     NVQPYQVKTTVTKRITETTIPSGSITNTTVPLKKYQESQQSTTSTTIQKVILPWQTTK
                     PVNMPGVAISTVTKPGLLYKSKECASDGEPILKVVGIGITEKPPTKCCEGLKLCSFLV
                     TDEKEFIEYQGVLSQILGYCKISCPENDTTKDLLPNPTKPKTLLESISDWFKPQVKEC
                     YTNKDCENKYCIKYPSSFIPLNNNVSTSIIPLCNYYCSTEGKCEKLDLGIITPNATST
                     SDTIEPNMPITDISCKDSSECILCNGACFSKKLWSNMEQLGIKCEVNATDKNKKCECN
                     AQNICMLVNLSDTSTSTTSRSNITLPNTCTNVYSPVCGYDNKTYQNRCELDRANIQFK
                     YTGVCTTTTTKALPQPGRCFTNSDCV"
     gene            1286..1912
                     /locus_tag="P5241_01355"
     CDS             1286..1912
                     /locus_tag="P5241_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HRV32212.1"
                     /translation="MKAEVEKSLGFYYPFVYKKPANLNCVCLSSAPGNSMLGFYCSAE
                     PVGVIGIPSTSTTTITVPTSPSSSTTTSTTMTTIPQYSCECPDIYQPVCGIDNKTYNN
                     ACNAGCVHIQIKREGVCEEAPNVPSTTIPQYSCECPDIYQPVCGIDNKTYNNACNAGC
                     VHIQIKREGVCEEAPIVPDTTIPPNIIPPTTIPIITPPTTQPLICIAL"
     gene            1916..2014
                     /locus_tag="P5241_01360"
                     /pseudo
     CDS             1916..2014
                     /locus_tag="P5241_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
     gene            2223..3536
                     /gene="murA"
                     /locus_tag="P5241_01365"
                     /pseudo
     CDS             2223..3536
                     /gene="murA"
                     /locus_tag="P5241_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
     gene            3607..4662
                     /gene="queA"
                     /locus_tag="P5241_01370"
                     /pseudo
     CDS             3607..4662
                     /gene="queA"
                     /locus_tag="P5241_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
     gene            4730..5866
                     /gene="tgt"
                     /locus_tag="P5241_01375"
                     /pseudo
     CDS             4730..5866
                     /gene="tgt"
                     /locus_tag="P5241_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
     gene            5875..6465
                     /locus_tag="P5241_01380"
                     /pseudo
     CDS             5875..6465
                     /locus_tag="P5241_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            6791..7246
                     /locus_tag="P5241_01385"
     CDS             6791..7246
                     /locus_tag="P5241_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32213.1"
                     /translation="MARQKGLTIPIVYNTNAYDKLEDIKSLKGLIDIYLPDYKYGDDN
                     LGIKYSRCINYSKVAQKNILEMLDQVGEDNIIVRHLVIPNNLDNTFEALKTIRSFSPN
                     ICLSLMMQYNPLYQAKNYPEINRKLSKDEYKRVMQYVEQLDFQTGYFQP"
     gene            7452..7826
                     /gene="speD"
                     /locus_tag="P5241_01390"
     CDS             7452..7826
                     /gene="speD"
                     /locus_tag="P5241_01390"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRV32214.1"
                     /translation="MEYGKHLLVEVITKNYETLTRIEDIREFFVQIVKELNFQVIAEP
                     FFYKFPSRIKKMQGGITGMYIIAESHLAIHTWPESKYFAFDLFSCRDFDEKQVIEIIQ
                     NCFLVDRMYFKVILRGIQNNFK"
     gene            complement(7935..8741)
                     /gene="miaA"
                     /locus_tag="P5241_01395"
                     /pseudo
     CDS             complement(7935..8741)
                     /gene="miaA"
                     /locus_tag="P5241_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
     gene            8859..9014
                     /locus_tag="P5241_01400"
     CDS             8859..9014
                     /locus_tag="P5241_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32215.1"
                     /translation="MSKLYFGYFVDICIIQVSRVCFAITEASAIEKILSSPFIIGFWL
                     YGKCGIV"
     gene            9546..10457
                     /locus_tag="P5241_01405"
     CDS             9546..10457
                     /locus_tag="P5241_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV32216.1"
                     /translation="MQFTHLHVHTHYSLLDGLCKIPNLLDRAKELGMNSMAITDHGNM
                     YGVVEFYDSAKERGMKPIIGCELYVAPRRLYMKEPHLDSFNFHLTALIKNAEGYKNLI
                     KLVSIAHLQGFYYKPRIDKELLQQYHKGIIFLSGCKKGELAQTILFKGKDKAKTILQE
                     YLDIIGKEDFYLELQYHKEDEETQKVNAVLLEFAKEFNIKPVVTCDTHYILKEDKETH
                     EVLLAVQSDADINDQDRKLSMSKFDLSLIDPKELIEAYKDTPEVIENTQEIVDRCNFD
                     FVKNKLFFPKFVAPDNKTSEDYLNELA"
     gene            10491..11732
                     /gene="dnaE"
                     /locus_tag="P5241_01410"
                     /pseudo
     CDS             10491..11732
                     /gene="dnaE"
                     /locus_tag="P5241_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            11844..12734
                     /locus_tag="P5241_01415"
     CDS             11844..12734
                     /locus_tag="P5241_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32217.1"
                     /translation="MASLLTSDYGDLDRVAIEVRECKRMGIKVLPPNVNYSFPEFGVS
                     KNNEIYFALSAIKNVGDNVAQAIAEERKQNGQYKSLEDFLTRLPQGVINKKSLESLIK
                     AGALDDFGDRIVLFNSLDKLLDFNIKKHNEANNKQSGLFASEIVDKIAFVMPSNNNKT
                     ESLEQKNLKLQWEKELLGLYISDHPLNHLGKVKQMSNVVSQINPLTMLGKTIRVCGIV
                     GNTRKILTKNGEVMYFSKLSDANKEIEMVIFPKTLKNIEKNGGASIADNKVIIATGRL
                     DRRNDSIQLICDNIIDVKEV"
BASE COUNT         4625 a         1531 c         2220 g         4552 t
ORIGIN      
        1 tagtataacc agaacatcta aaaatactat gaattttgaa gacatccaaa taaatgataa
       61 aataactgta actggcgaac taataaaaga agcaagtaaa gatgtaagag gtgaaattat
      121 tgctaaattg attaatgttc aaccatatca agtaaaaact actgttacta aaagaattac
      181 agaaactaca ataccctctg gttcaattac caatacgaca gttccgttga aaaaatatca
      241 agaatctcag caatctacca cttctacaac aattcaaaaa gttatattac catggcaaac
      301 aactaaacca gttaatatgc ccggtgttgc tataagtact gtaactaaac ctggattatt
      361 atataaatca aaagagtgtg ccagcgatgg agaacctatt cttaaagttg ttggtatagg
      421 tattactgag aaaccaccta cgaaatgttg cgagggatta aaattatgca gttttcttgt
      481 tactgatgaa aaagaattta ttgaatatca aggtgtgtta tcacaaatac ttggttattg
      541 taaaatatca tgtccagaga acgatacaac aaaagatttg ttgccaaatc caactaaacc
      601 aaaaactctt ttggagtcta tttctgattg gtttaaacca caagtaaaag agtgctatac
      661 caataaggat tgtgaaaata aatattgtat aaagtatcca agctctttta taccgttgaa
      721 taataatgtc tctacttcaa taattccttt atgtaattat tattgtagta ctgaaggtaa
      781 atgtgaaaag ctcgatctag gaattattac ccccaatgct actagtacaa gtgatactat
      841 tgaacccaat atgcctatta ccgatatatc atgtaaagat agttctgagt gtattttgtg
      901 taacggtgct tgtttttcta aaaaattgtg gtctaatatg gagcaattag gtataaaatg
      961 tgaagtaaac gcaactgata agaataaaaa atgtgaatgt aatgcccaaa atatttgtat
     1021 gcttgttaat ttatcagata cttctacgag tacaacttcc agatccaata taactttacc
     1081 caatacttgt acaaatgtgt attcaccagt ttgtggatat gataataaaa cttatcagaa
     1141 tcgttgtgaa ttggatagag cgaatataca atttaaatat acaggagttt gtacaacaac
     1201 tactactaaa gctttaccac aacctggccg ctgttttact aattctgatt gtgtatgatt
     1261 tggcgataga tgtatagcta caaatatgaa agcagaagta gaaaaaagtt taggatttta
     1321 ttatcctttt gtatataaaa aaccagctaa tttaaattgt gtgtgtttgt cttctgctcc
     1381 tggtaatagt atgttaggct tttattgttc tgcggaacca gttggagtaa taggtatacc
     1441 ttctacaagt actactacta ttactgttcc aacttctccg tcttctagta ctactacttc
     1501 aacaacaatg acaacaattc cacaatattc ttgtgaatgt ccagatattt atcaaccagt
     1561 ttgtggtatt gataataaaa cttacaataa tgcttgtaat gcaggttgtg tccatattca
     1621 aataaaacgc gaaggagtat gtgaagaagc tcctaatgtt ccgagcacaa caattccgca
     1681 atattcttgt gaatgtccag atatttatca accagtttgt ggtattgata ataaaactta
     1741 caataatgct tgtaatgcag gttgtgtcca tattcaaata aaacgcgaag gagtatgtga
     1801 agaagctcct attgttcctg acacaacaat tccacccaat ataattccac caactactat
     1861 accaataatc acgcccccaa ctacacaacc tttaatatgt attgctttat gagatcctgt
     1921 gtgtggtgta gatgggcaaa cttattcaaa tgattgttat gcgcaggctg ctggtgtagc
     1981 tgtaaaatgt caaggtgaat gcccatgcga gtaatttatc aattagtatt agcagttggc
     2041 aattagctat tattagttaa tagaagcaaa aattcgcgtt aatctgtgta ttatttttgt
     2101 aaatcagcgt attattaata tctaaatttt tgtggtcatt tggatgtatt tgtaaatttg
     2161 gattgtgttt attaaaaatt ggactttttt agttttcatt atttataaat ttaaatttta
     2221 ttatgatacc caaatataca attgttggtg gtaataaatt aaaaggagaa attatattag
     2281 caggggcaaa aaatgtttcc accaaagtta ttattgcttc gttgttagct gatagtaaat
     2341 ctgtttttga aaatatgccc cgaattagcg atgtcgagac aactttgttt atttgtaagt
     2401 cattaggagt taaatataaa tgattagata aaaatgtttt agaggttgat ccaagtcata
     2461 taaaatctta taaaattcct gtggaatata gtggattaaa tcgaatgcca atattatttt
     2521 ttggtccatt gctttataga tttggtaaag cagaagtgcc aacaatagga ggttgtagta
     2581 ttggtgcacg ccctgttgat ctacatgaaa gtgcttataa agatttgggt gcagaaatag
     2641 aatatagcaa tcattattat agaattaaat ctcctaaagg gttgaaggga acaattatca
     2701 aattaaatta tccgagcgtt ggagctacgg aaaatatttt gcttggttca gtattagcca
     2761 aaggcaccac agtaattcaa aatgcagcta ttgagccaga ggtagaagag cttgctttat
     2821 ttttgcaatc aatgggtgca atgatttatc aagatgtcaa tagaacgtga attataaagg
     2881 gtgttcaaca attacatgga gctaattata aaataatgtt tgatagaaat gaatttgtat
     2941 cttttgcttg tttagctatg gccaatagag gagatatttt gataaaagga gtagaacaaa
     3001 gatatgtaat gactttttta aacgctgcga ggaaaatagg tgccaatttt accatatata
     3061 atgatgcgat taggtttcat aataatgaaa atgttaagtt taaatctata gctgtggaaa
     3121 cagatgttca cccagggttt atgactgatt gacagcaacc attttgtgta gttttaacac
     3181 aagccaatgg gacttcgatt attcatgaga ctgtttatga aaatcgtttg gggtatacag
     3241 atgctttaaa taaaatgggt gcgaatattc aattgtttcc tgaatgttta ggtagtaaac
     3301 cgtgtagatt tgtaaataaa gattatttac atagtgcttt gattactgga ccaacggcat
     3361 tgtcaggagc gaaaattgaa gtaccagatt tgcgtgcagg tttttcttat gttatcgctg
     3421 ctgtgatagc aaaaggtaag tctgaactaa taggtgtaga aaaaatacaa cgaggatatg
     3481 aagaccttaa agatagacta caaaacattg gcgctataat agatgaaaca aaataatcga
     3541 ttatataata acataaattg ttgataataa agtatggtgt ttttaatatg aaattaaaat
     3601 gtgtgtatga atatacagtt gtttgattat gaattgccca aaaatttaat tagtcaaaaa
     3661 ccaattgtac ctcgagacca ttgtaaattg ttggtgtaca acaaagaaac caaaactata
     3721 aaacataaaa aattttatga tattgtagac gagttagata aaaatagtgt tttggttttg
     3781 aatgatacaa aagtataccc agcacgtttg tatgggaaaa aacttactgg tggtaaaata
     3841 gaaatatttt tgcttggcac tactaataat aaatttagtt tagacaattt gtctaatagt
     3901 tgaaatattt tagtaaataa aaaagtaaaa gataaagaag aaataaaatt ttatttgaaa
     3961 aataaagtag ttttgtctgg gatcattgta aataaaggcc aagatactga tttaaaaata
     4021 gaatttaata ccaaagggag aaacttaatg cataaaattt ggcaattagg gcaaatacct
     4081 ttgccgcctt atattaaaaa ttataataag cataaaggta aatattatca aactatttat
     4141 gcaaaacatt ttgggtcttg tgctgctcct acagcaggat ttcatttcac taaaaattta
     4201 ttaaataaaa taatcaaaaa gggtattaaa atagaatata ttactcttaa tgtaggaata
     4261 ggaactttta aaccaattaa aacacaaaat atagaacagc atcatatgca caaagaatat
     4321 tttgaagtat ccaaaactac agcaaaacat ttgaatacat ataggcaaca aggtaaaaaa
     4381 attgtagcag ttggaactac ttgtgtgcga gtgttggaaa ctattgttaa agacaatcaa
     4441 tttgtttctg gcgctgggtt tacagatatt tttatttatc ctggctataa ttttaaaggt
     4501 gttgatcagt ttatcactaa ttttcacttg ccacgttcaa ctcctttgat gatggtggcg
     4561 gcatttgtgg ggttaccaga agaaactttg agaatatata aggaagccat acgtaaaaaa
     4621 tataaatttt atagttttgg cgatgctatg tttataaaat aaataagata atatataaca
     4681 attaataatg ggtaaataat aattttgaat gttataattt ttaatcaaaa tgaaagtttt
     4741 tgaaatcaaa tataaatcta aaaataccaa agcaaggatt ggagttttac acacggctca
     4801 tggtgtgatg gaaactccag tttttatgcc acctgctagt aaagggtatt taaaaagttt
     4861 ggatactttg gatataaaca ataccaaaac ccaaattatt ttgattaatg cctatcattt
     4921 atttttaaat cccggtgtac attttattca aaaatacgga ggaattcata gttttatgaa
     4981 ttgaaataaa cctataataa gtgattcagg agggtttcag gtctgaagtt taaataataa
     5041 aaataacaaa ttagcccgta taaaagatga cggggtatat tttatttctc cttatgatgg
     5101 gtttgaatgt tttattaatg ctcaaacatc tatacagata caacataatt taggggctga
     5161 tattgttatg gcgtttgatg aatgttcaaa agataaagga agtaaaaaag atattaaaaa
     5221 atcattagat ttaacaaata aatgattaaa acaatcgtta gaaactcata tttatcttaa
     5281 taaaagaaaa aataataaag tattattttt tggtatagtg cagggaggta gatacaaaga
     5341 tttaagatta caatctcttg aatttgtaaa atcattagat acagatggta ttgcttttgg
     5401 aggagaaact attggatata atatgaaaga tacaaaaaaa ttattgaatt atttagtgcc
     5461 ctttacaata gaatataagc ctcgatatgc tatggggctt gggggtaagc cacaagatat
     5521 tgttgatgca attattttag gtatagatat gtttgattgt gttagccctg ctaggattgc
     5581 tcgacacgga gaattgttta ctggtgtgtt agattttaat acttgaaaaa tcaaatctaa
     5641 ggtaaaaaat aatattgttc gaattgctaa tagtacatat agaaatgata aaaaaccaat
     5701 tgatgagcat tgtaaatgtt ttacatgtca aaattattct cgggcatact tacggtattt
     5761 atttatgcaa cacgagccat tgtatatgag attggctact attcataata tttattttat
     5821 aaataatatt atacaagaaa ttaaaaatta tataaaaaac gaataaggat tataatgaat
     5881 aaaaaaattt tattacatac ttgttgcgcg ccctgtgttt tgccaatatt ggattatttt
     5941 aaacatcaaa aaattttaga taatgttgtt ttatattttt ttaatcccaa tattatacct
     6001 aagcaagaat atgacaagag attgttttat gttagaaaag taggtaagta ttataatata
     6061 aatgttcttg aaggtgaata tacaaataaa gaatgattga tgtacttaaa aaatcatttg
     6121 gatttggatc taacaagtta taaagaaaat ggacctcggt gcgatgtttg ttttacttat
     6181 cggttggaaa aatctgtttt atttgctaaa caaaataatt ttgatagttt ttgtactact
     6241 ttacaagtaa atttatataa aaatactaat tttattattc aaaaatctaa agaattatgc
     6301 aaacattatg gaatacaatt tattgatcta ttgttagata aacaagaagc tcacaaaaaa
     6361 gaaaaagaat tatgtcaaaa attagatatt tatagacaaa aattttgtgg ctgtaattta
     6421 aacatagatt ataaatttat taaaaatcaa aatgtaaaaa tttaaataac aaatcaaaaa
     6481 tagggaatct ggttttgaaa taataaaaat aataaagtat aatattatga aaatagcttg
     6541 gtttggaaaa cattttgggg aagaaccccc aattagtggt gataaaggat cagggacaat
     6601 atttttttct ggatgtaatt tacattgttg tttttgtcaa aattgacaaa tttctcagca
     6661 atgattaggt aaagaataca ctgtcgaaga attagtagat ataatgttaa aattacaaga
     6721 tcaaggatct ctgaatatta atttagtaag tcctactatt tgagtatttg atattaaaca
     6781 agcaatagtt atggcccgcc aaaaaggtct aactattcct atagtttata ataccaatgc
     6841 gtatgataaa ttagaagata ttaaaagttt aaaagggttg attgatattt atttgcctga
     6901 ttataaatat ggagatgata atttgggtat aaaatattct cgatgtataa actattcaaa
     6961 agttgctcaa aaaaatattt tagaaatgtt agatcaagtt ggtgaggata atataattgt
     7021 gcgtcatttg gtaataccta ataatttaga caacactttt gaggccttaa aaactattcg
     7081 ttctttttcg ccgaatattt gtttgagttt gatgatgcaa tataatcctc tttatcaggc
     7141 aaaaaattat ccagaaataa atcgcaaatt gtcaaaggac gaatataaac gagtgatgca
     7201 atatgtagag caattggatt ttcaaactgg gtatttccaa ccctgagaag aaaatgccag
     7261 cgaagttttg atacctgatt tttcaaaacc taatcctttt gtgtaaaaac aattttataa
     7321 aaaattataa taaacttata aaaaattata ataaactatt gtttttgtat gtaaaatatg
     7381 atattatttt taagtataaa aacagtctta ttaaaaatcg aaacaattaa taaataatta
     7441 acatttagta aatggaatat ggtaaacact tattagtcga agttataaca aaaaattatg
     7501 aaacacttac taggattgaa gatattagag aattctttgt gcagattgta aaagagctta
     7561 actttcaagt aattgctgag cccttttttt ataaatttcc ttccagaatt aaaaaaatgc
     7621 aaggaggaat aactggtatg tatattattg ccgagtctca cttagctatt catacttggc
     7681 cagaatctaa atattttgct tttgatttgt tttcttgtcg tgattttgac gaaaagcaag
     7741 taatagaaat tattcagaat tgttttttag ttgatagaat gtattttaaa gttattttga
     7801 gaggtataca aaacaatttt aaataattta ttaaggcaaa aaatattaaa ttatggcttt
     7861 gacaatcaac gttttactat tttgattgcc tgtttatcgt tattaatata ttgaacattg
     7921 gggaattttt taaatcaagt taattgacgt ttcgcaaaat caattatttt tttatataaa
     7981 gtatcataca tttcttgata tgatattttt ttatctaaat ataaattgat atagcgatat
     8041 tctaaaccaa gatcataaag acgttttgaa gatatatttt gtttttttaa tttttttact
     8101 tccttaatca taccttgttt taatctttta tcaaggcgtg ttttgattaa atgttttatt
     8161 tcctctttgg atttctgggt gcttagaata agaatatctg atgtgagagg ttgtttttta
     8221 agtggttgta ttggtttttc ggtagtataa ataatttcta atgcccgaat tattcgtcgt
     8281 ttattgtttg aatctatatt gttggctcta acaggatcta atttttttaa ttcttgtcat
     8341 aaatctgtta aaggccttgc tgataattgc gctcgcagtt taggatttgt ttttattttt
     8401 gggaattgta agccttcaat aacactaata acatataaag gagatccgcc acaaataata
     8461 ggtagttttt gttgccgtca tatcttttta attattttgt ttgcttgttg tgtatattgt
     8521 gctacagtat attgtttttt aacagaggct ttattgatca tataatgctt tacttgtttt
     8581 tgttcttgtt tggttatttt accagtacca atatcaaggt ttctgtatat ttgtcttgag
     8641 tcagctgaaa taatttctcc gttaaatttt ttagcaattt tgattgccaa attagattta
     8701 ccagtggcag ttgcccccac tataactaat aattttttca tttttatatt tgtttattta
     8761 tgatttttgt ttaatgtaga aatgattgtc gatgtaaact acaagggcca tattttttta
     8821 taagtttttt atgttgttcg gtgccatatc ctttgtgaat gtcaaaatta tattttggat
     8881 attttgtaga tatttgtatc attcaagtat cgcgagtatg ttttgcaatt acagaagcca
     8941 gtgcgataga aaaaatttta tcatctcctt ttattatagg tttttggttg tatggcaaat
     9001 gcgggattgt ttgattacca tcaattaata ttatattggg gattattttt gatttattta
     9061 taatttgtga gatacatttg tcaaaacatt gaaaaatact tttttgaatc cctattgtat
     9121 ctattgtttg attgtcaatc attgaaatac taaaaataat ctggggtaaa ttgatagctt
     9181 gctcataaat tttttgtcgt atttgtgggg atagtttttt tgaatctttg acaagggcta
     9241 caaatttatt gggcagtgga taatttgggc gaattaagac tgcacaacta caaactggtc
     9301 ctgctaatgg accgcggcca acttcatcta tccctataac gaatggatag ttttgttttc
     9361 aatatatttg ttcgatatca agtttcataa tattaaatct taaaacatta taaatgtcaa
     9421 ggttgttttt ttcttagaaa tagggtatat tatttatact tataataagt taagcagtgt
     9481 tgttattttt atttccccga tatttcatta ttaatattat cataaaaagc tttaaatttc
     9541 aaaggatgca atttactcat cttcacgtac atactcacta tagtttgtta gatggtttat
     9601 gcaaaattcc taatttattg gatagggcta aggagttggg catgaattct atggctatta
     9661 ctgatcatgg taatatgtat ggggttgtgg aattttatga ttctgcgaaa gaaaggggca
     9721 tgaagccaat aattggatgt gaattatatg ttgcacccag aagattgtat atgaaagagc
     9781 cacatttaga tagttttaat tttcatttaa cagctttaat aaaaaatgct gagggatata
     9841 aaaatttaat caaattagta agtattgcac atttgcaagg attttattat aaaccaagaa
     9901 ttgataaaga attattacaa caatatcata aaggtataat ttttttgtca ggatgtaaaa
     9961 aaggggagtt ggctcaaaca attttattta aaggtaaaga taaagctaaa acaatattac
    10021 aagaatattt ggatattatt ggtaaagaag atttttattt ggaattacaa taccataaag
    10081 aagacgaaga gactcaaaag gtaaatgctg ttttgttgga gtttgctaaa gaatttaata
    10141 tcaaaccagt agtaacttgt gatacacact atattttgaa agaggataaa gaaactcatg
    10201 aagttttgtt ggcagtgcaa agtgatgcag atataaacga tcaagataga aaattgtcta
    10261 tgtccaaatt tgatttatct ttgattgatc ccaaagaatt aatagaagca tataaagata
    10321 ctcctgaggt tattgaaaat acacaagaaa tagttgatcg ttgtaatttt gattttgtaa
    10381 aaaataaatt attttttcct aagtttgtgg ctcctgataa taaaacttcc gaagattatt
    10441 taaacgaatt ggcgtgaaat caatttcctt tatattatac aaaagataat aaagaagcga
    10501 gagatcgtgt tcaatacgaa ttaggagtta tcaagactac aggttttata gattattttt
    10561 taattattag agatattaca caatattgtc gcgaatcaaa aattccgttt aatacacgag
    10621 gatcggctgc tggcagtgtt gtttcttata ttttgggtat ttctgcgctt gatcctatta
    10681 aatatgattt atattttgag agatttttga accctgatcg tattagcccg ccagatatcg
    10741 atcttgatgt ggcagataaa gatagaggtc gggttataga atatattacc aataaatatt
    10801 cttctaataa tgtttcgcaa attattactt ttggttttat gaaagctaga atggctgtta
    10861 gagatgtcac gcgtgctttg ggtttaccat atgctttggg tgacaaattg tcaaaaatga
    10921 ttcaggtaca tatggaattg gatgatgcct taaaaaatat tccagaattt aaagaagaat
    10981 atgataataa tcccgatgca agacgagtaa ttgatgtttc gaaaaaatta gaaggtgtag
    11041 ccagacatgt gtcaactcat gcagctggag tagttgtgag ccctgaacct ttagataatt
    11101 ttgttccttt gcaacattcg agtagatcag agaaagatat tatagtacaa tatccgatgt
    11161 atcatataga agctttgggt ttgttaaaaa tcgatatatt aggtttggct aatttgtcta
    11221 ttataaaaca aagtttaaga gttataaaaa agctttataa taaagatatt gatttagatg
    11281 cccttgggtt tgaagatcct aatgtattta gattattacg aagaggtgat actattggaa
    11341 tttttcaaat ggaaagcgag ggtatgagaa gagtggcgat tgatacgaag attgataatt
    11401 ttgaagattt agcagcagtt attgccttat atcgtccggg gccaatagaa tttatttcac
    11461 agtatataaa taataaatta ggtaaaaccc agtgtaaata tctggatcca agaatggaac
    11521 ctatattagg taaaacttat ggtgttatgg tgtatcaaga gcagttaatg cgattagctc
    11581 acgatttagc tttgtataca atgggcgaag cagatatttt gcgcaaagca ataggcaaaa
    11641 agaaaaaaga attattgctg gaacaagaac aaaaatttat aaaagggttg gttgctaatg
    11701 gtatgtctat agataaagca aaacaattgt gagaatgagt tataccgttt gctagatatg
    11761 gttttaataa agctcatgct atttcttatg cgcgtgtagc ttatcaaaat gcttgattaa
    11821 aagtttatta tccaattatt tttatggcat ctttgttaac ttcggattat ggagatttgg
    11881 acagggttgc tatagaagta agggaatgta aaagaatggg gattaaagtg ttaccaccta
    11941 atgtaaatta ttcttttcct gaatttggtg tatctaaaaa taatgaaatt tattttgctt
    12001 tgtcggcaat aaaaaatgta ggagataatg tggcacaggc aatagctgaa gagagaaaac
    12061 agaatggaca atataaaagt ttagaagatt ttttgacacg tttgcctcaa ggcgtaataa
    12121 ataaaaaatc attagaaagt ttgatcaaag caggcgctct cgatgatttt ggcgatagaa
    12181 ttgttttgtt taattctttg gataagcttt tggattttaa tattaaaaag cacaatgaag
    12241 caaataataa gcagtctggt ttgtttgctt cagaaattgt tgataaaatt gcttttgtaa
    12301 tgccatcaaa taataataaa actgaatctt tggaacaaaa aaatcttaaa ttacaatggg
    12361 aaaaggagct tcttggtctt tatatttctg atcatccgtt aaatcattta ggcaaagtaa
    12421 aacaaatgtc aaatgtagta tctcaaataa atccgttgac aatgttagga aaaactattc
    12481 gggtgtgtgg tattgttggc aatactagaa aaattttaac taaaaatggt gaagtaatgt
    12541 atttttctaa attatctgat gccaataaag aaattgaaat ggttatattt cccaaaacat
    12601 taaaaaatat agaaaaaaat ggtggggcaa gtattgccga caataaagtt attattgcta
    12661 ctggcagact tgatagacgt aatgatagta tacaacttat ttgtgataat attatagatg
    12721 taaaagaagt ttaattttgc tgctatatct ttgagtaatt tattaagtaa taacttattt
    12781 aatttatatt gtgattgtta gtgttttata ttattgtgtt tatagccgtt ttttctctgt
    12841 ttatttctct tcgtttttta gatgttttta gctcttgaca taatatgtat aatgtgctat
    12901 gattatattg tatcatataa attatgat
//
LOCUS       DAOTJA010000012        12812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_5336_length_12812_cov_6.408011, whole genome shotgun sequence.
ACCESSION   DAOTJA010000012 DAOTJA010000000
VERSION     DAOTJA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5336_length_12812_cov_6.408011"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="P5241_01420"
     CDS             complement(<1..188)
                     /locus_tag="P5241_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HRV32218.1"
                     /translation="MPNLLDNLKTLLQQDERLVSEGELLKNKIIELVLKMDKDLIELL
                     LSDKQMKEVFFTEVGKAT"
     gene            complement(332..511)
                     /locus_tag="P5241_01425"
     CDS             complement(332..511)
                     /locus_tag="P5241_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRV32219.1"
                     /translation="MKFPQRAHASGHISGPELKQVVEFINPKTLMPIHTEYPKIFQQF
                     HKNVKIVKPNETVYL"
     gene            complement(731..1060)
                     /locus_tag="P5241_01430"
     CDS             complement(731..1060)
                     /locus_tag="P5241_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32220.1"
                     /translation="MSAIKKQNIQIMICEGTRIKDDHKVSEKEVFNEAYKILQNKKGL
                     ILVDSSPTDLNRFNTLCELSQKLNRTLVIQPSHYYYLQFFNNNNYPIPGFDKTFVYAR
                     QKCSIRQ"
     gene            complement(1067..1753)
                     /locus_tag="P5241_01435"
     CDS             complement(1067..1753)
                     /locus_tag="P5241_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV32221.1"
                     /translation="MQISFYGGINEIGGNKILLDDQDTKIFLDFGKSYKGLSFYFEQY
                     LQPRVVHGIKDYLSLGLIPELNGVYREDLIDILQKDNYTKLSHQPPIVDGVLISHGHL
                     DHVGYIAFLDKRIPLYGSALTHLALKALQTTRPYTFENEILQFKPRTKKQDKKHKNKV
                     IVEKSAIIKRDYNITQDRKPFKIKNLTIVPYDVDHSIPGAQMFVIKTSIGNILYTGDF
                     RAYCTQEEYF"
     gene            complement(2036..2791)
                     /locus_tag="P5241_01440"
                     /pseudo
     CDS             complement(2036..2791)
                     /locus_tag="P5241_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(2896..3903)
                     /locus_tag="P5241_01445"
                     /pseudo
     CDS             complement(2896..3903)
                     /locus_tag="P5241_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
     assembly_gap    3995..4094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4095..4768)
                     /locus_tag="P5241_01450"
     CDS             complement(<4095..4768)
                     /locus_tag="P5241_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV32222.1"
                     /translation="MSIGDEYTLRKNLEDTKIYKNLLKILKNKHDLYANMFFAANSSD
                     FVFGGKTLKQVAKQFNLTIEQTLLKILSLKPKQILVYDNAVSNENIQLALKSPYCIVS
                     SNSTIHNTEKYYSDNFIHPRAFGTFPRLFDLYVKELSTLSFKEAIYKCTGLPSKKLNL
                     KTKGIIKLNNDADIVIFNPQTIKDTANIKNPFTAPKGIETVIVNGKLSIHKGQPLSSY
                     AGQILS"
     gene            complement(4895..5122)
                     /locus_tag="P5241_01455"
     CDS             complement(4895..5122)
                     /locus_tag="P5241_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32223.1"
                     /translation="MGVSFGLAYPNQQVVGLKEMIEASKLVTKYNGLVSIHLRSESEK
                     LLEAVDEVIDLARKTNANIEITHFKAYGKPN"
     gene            complement(5303..5731)
                     /locus_tag="P5241_01460"
     CDS             complement(5303..5731)
                     /locus_tag="P5241_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32224.1"
                     /translation="MLQKIHFYDRLFIYIKTLKPCMKYDILIKNGIIFDGTAKDAFMS
                     SLGIKNGKIIRIGNDILDKDAKNVINASNKYVCPGFIDINNSADHYLGILNSPKATNL
                     LAQGITTIILGHCGASLAPIVNGKLDFLNYWTTTQDINIS"
     gene            complement(6489..7595)
                     /locus_tag="P5241_01465"
     CDS             complement(6489..7595)
                     /locus_tag="P5241_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV32225.1"
                     /translation="MLKKEIAKIEHKTKKKNSSSFIQEEVTSEEIAKCVSQWTGIPVE
                     RMLENEKNKLANMENILSSRVIGQDEAISAVSRAIRRSRAGLSEEDKPIGSFMFLGPT
                     GVGKTELAKALAEFMFDDEKSLIRIDMSEYMEKHSVARLIGSPPGYVGYEEGGQLTET
                     IRHRPYSLILFDEIEKAHPEVFNILLQILDNGRLTDGKGRLVNFKNTIIIMTSNLGGE
                     YISQMSSLGFNNITAMPEHNSYEEQKQRLKDKINEVLRDSFRPEFLNRIDEIIIFNPL
                     QPKDIEKIVDIQIQKVVNRLKIQNILLNVDDSAKKWLALRGYDPQFGARPLKRLIQKS
                     VLDPLADKIIKGEYKNKKQIYLTASKDGLVFKAK"
     gene            complement(7731..8546)
                     /locus_tag="P5241_01470"
     CDS             complement(7731..8546)
                     /locus_tag="P5241_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV32226.1"
                     /translation="MQILSRRTKNNPVLLGEPGVGKTAIVEGLAQKIIENEVPDNLKN
                     KEIISLDIGALVAGTKFRGEFEDRLKSVLKEIKASEGKYIIFIDELQTIVGVGAAEGS
                     IDASNLLKPALARGELHLIGATTYKDYRNSVEKDPALERRFQPVIIDEPSIEDTITIL
                     RGLKPKYEVFHGVKITDSAIRDAVNLSVRYIPDRFLPDKAIDLIDEAASHLKLQMNSV
                     PPTIANIQKEIRTLEIEKEALKKEEDKESQKRLSVVNKKLETLKKEEDKLLQQ"
     gene            complement(8706..9125)
                     /locus_tag="P5241_01475"
     CDS             complement(8706..9125)
                     /locus_tag="P5241_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRV32227.1"
                     /translation="MPTIPQFNPEAQDVIERAQQLAVEYNHEEIKVLHLLLAIAEQDN
                     SFFDVVVSELKLNKDTIINDIQKQLEQIANAFKMQNFGQIALDQEVIMVLEKSARYSK
                     ETNSKYISPVHILLAISTVNSSAKTLLEKYKINTDVI"
     gene            complement(9317..10159)
                     /locus_tag="P5241_01480"
     CDS             complement(9317..10159)
                     /locus_tag="P5241_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV32228.1"
                     /translation="MIDSNGYIITNKHVVQDTKAEYTVLLNDGTEYSATVIARDPLED
                     FALVKINKTGLVPLILGNSDNLMLGQTAIAIGNALGELQNTVSVGVVSGLHRNITAYD
                     TTGEPTTLTDLIQTDAAINQGNSGGPLLNLNGEVIGVNVAMEQGAQNIGFAIPISKIK
                     GIINQAISTGKISVPFIGIHYTIIDKDLMKEKNLPVDYGALIGKGANNDPAIVKGSPA
                     EKAGLKENDIILSINNIKITAQNSLSSIVRRFNIGDKVKLEVLRDKKTIYLDLILGSK
                     DSTD"
     gene            complement(10850..11224)
                     /locus_tag="P5241_01485"
     CDS             complement(10850..11224)
                     /locus_tag="P5241_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32229.1"
                     /translation="MTSNISTDEAFILSIMRNPLFIYTFTICGVLVLAIFTFCIIASI
                     EVANGEKSWKKAFQKSWHLFSNAFLLTVLCILLINIGFILFIAPGVFALFLLSLVYPI
                     TAIKKSKPIKTIQQSCHLNITH"
     gene            complement(11417..11944)
                     /locus_tag="P5241_01490"
     CDS             complement(11417..11944)
                     /locus_tag="P5241_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV32230.1"
                     /translation="MNKHKILLVEDELFLSSLLQMKLEQEGFQIERAKDGEEAIQILE
                     TFMPDLVLLDLILPKKNGFEVLESMKQDPRFANIPVIIISMLGQDSDIARGKSLGVID
                     YIVKARLSVDELVNKVKDKVKLSIDGIANNNSSVSVTASAPTIQSSQSSTPLSSPATP
                     TANTPVNNSTQNTTK"
     gene            complement(12294..>12812)
                     /locus_tag="P5241_01495"
     rRNA            complement(12294..>12812)
                     /locus_tag="P5241_01495"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4333 a         1866 c         1811 g         4702 t
ORIGIN      
        1 attgttgctt tacctacttc tgtaaaaaac acttccttca tctgtttatc ggataaaaga
       61 agttcaatta agtccttatc cattttcaac acaagctcaa taattttatt ctttaaaagc
      121 tcgccctcgc ttacgagacg ttcgtcttgt tgaagtaaag tttttaaatt atctaataaa
      181 tttggcataa tttaataatt cagatatatt tttttatttt atcagcattt tgctgttaaa
      241 aaattcttta tctatataat atattgtatt aagataattg tagcattttt tggaataaaa
      301 tgcaatcttt tgtagttttg gtagttttgt attataaata tacagtttca ttgggtttga
      361 caattttgac atttttgtgg aattgttgaa agatttttgg atattctgtg tgtataggca
      421 taagtgtttt ggggttgata aattctacta cttgttttaa ttctggacct gaaatatgac
      481 ctgaggcatg ggctctttga ggaaatttca taccaaaaaa tttaattcaa tttttaaatc
      541 gttcttctga taattccatc tcttcactat gtggctcagt agtagaacgc aaaaaataac
      601 tcccctttat gggatgtata tcaattaatt cttggatttg ataaaaatta ataattaaca
      661 tatattgttt ttgattgtta gaaatatcat cggcggtgat aacattgtct aaatcaaggg
      721 tttcttgttc tcattgtcta atagagcatt tttgtctggc ataaacaaaa gttttatcaa
      781 aacctggtat tgggtagttg ttattattaa aaaattgtaa ataataataa tgggagggct
      841 gaataacgag cgtgcgattt aatttttgcg acaattcgca taaagtgtta aatctattta
      901 aatctgttgg cgaagaatct actaaaatta atcctttttt gttttgtaaa attttgtatg
      961 cttcgttaaa aacttctttt tctgaaactt tatgatcatc tttaattctt gttccttcgc
     1021 aaatcattat ttgtatgttc tgttttttga ttgcggacat ttgttgtcaa aaatattctt
     1081 cttgggtaca atatgctcga aaatctcctg tatataaaat atttccaatg cttgttttga
     1141 ttacaaacat ttgcgcaccc gggattgagt gatcaacatc ataaggaact attgttaaat
     1201 ttttgatttt aaagggtttg cgatcttggg taatattata atcacgcttt ataattgcgc
     1261 ttttttctac aataacttta tttttgtgtt ttttatcttg ttttttggtt cttggtttaa
     1321 attgtaatat ttcattttca aaagtatatg gacgagtagt ttgtaaagct tttaatgcca
     1381 agtgcgtaag tgctgatcca taaagaggaa ttcgtttgtc taaaaaagca atatatccaa
     1441 catgatcaag gtgtccatgg gaaattaaaa ctccgtctac tataggtggc tggtgtgata
     1501 atttagtata attgtctttt tgaagaatat caattaaatc ttctctataa acgccattta
     1561 attcaggaat aagacctaat gataaataat ctttaatacc atgtacaaca cggggttgta
     1621 aatattgttc aaaataaaat gatagccctt tataactttt accaaaatct aaaaatattt
     1681 tggtatcttg atcatccaaa agtattttat tcccccctat ttcgtttata ccaccataga
     1741 atgaaatctg catattgtaa tttgtaataa tgtttttata atgtattgat aattatatta
     1801 tatttttaat atttaaaata ttttaaatta tttaatgaat tgctaaataa aaattttcaa
     1861 gcataagctt tttatttaaa ttgtaattgg ttgtataatt atataattgt aaaacatttt
     1921 tgagaagttg ttttattagg gtagaattag ataataataa cgataggtca gtttttagat
     1981 atttgtttca aaaatcaata tttttgtaca ctaaaatttg ttgtaaaaac cctaatcaat
     2041 cattaactaa ctgagataaa ttatattcgt ctttgtttgt tatattttgg ataatatttg
     2101 ttgcttcaac aaaattagaa ttcaataaat caataaatat tcgaatatta ctaattttgg
     2161 tgttcaaata ttcggggttg ttgataagtt gaaatatttc gcctattttg ttttgtccca
     2221 attctaaata taaagttaat tgctcatgag gaatttggta ggtattgata agaaaatttt
     2281 tgagagattc atctggctct ttggcaaaac gaacaggtaa taaacgcgat aagattgttg
     2341 gcattaactg attaggaaaa ggagtaacta atattataat actatttatt gagggctctt
     2401 ctaaaatttt taatagagaa gattctgctt cgggagttag tttgtgagct tcgtcaataa
     2461 ttagaatacg attttcagat aattgaggtt tatacattaa aaaatttttt aaatctgaaa
     2521 tttcttcgat tttgatagaa ttggaagttg gttgaaatat tttaaaatca gggtgtcaat
     2581 tatgattaat ggccttgcac gaatcgcact cattgcaacc ttgccattga tgttttttac
     2641 ataaaatact cttggcaaat atttgggcaa tataaaactt acccagatgt tctccgccat
     2701 aaaaaagcat gctttgactt aaacgagtct caggatttag ctgttgttct aaaaattttt
     2761 gagtattttt gttgagcaaa agtgtagaca ttataattaa gtttttgatt cttaatttga
     2821 caatttttaa ataataacgt aatggtttaa cgtctaaaaa ttaaaaacag gacattaaac
     2881 aataaagacg tttaattatt tataatttat tactgttctt tgatatgttt gaagattatc
     2941 taaaattata cccgtgcctt tggcaacaca aaatagagca tcatccgcaa tatacactgg
     3001 tactcctgtt gaacgagtaa ttaattcgtc gatatttttt agcaaagctc ctccaccagc
     3061 tacaaacata cctcttgaca taacatcagc tactaattca ggtggtgttt ctgacaaaac
     3121 agttttgata gcttgtataa tgtcttttaa aacaggagac aaggcctcgg ctaattcatt
     3181 tgaatttata gttaaatttt ttggtagccc ttctgttaaa tctccaccgg taatttccat
     3241 ttcttgttta tcttttgtgg gtaaagcact agcgatttca atttttaaat tttcagctgt
     3301 acgttcaccg atagttatat tatacttttt tctaatataa tcagtaatcg cttcatccat
     3361 tttattacca gcaactctaa tagatgccca agtgactata ccacctaaag agataacagc
     3421 tatttcagta gtgcctccgc caatattaat aatcatattg ccagctggag catttattgg
     3481 gatacttgct cccaaagctg ccaaaattgg ttcttttact acatatacat cttttgcacc
     3541 tgattcatgg gcagcatcaa taatagcacg acgttcagtt gatgtaacac cacaaggagc
     3601 agaaataact aaatctggtt ttactaatct tcaatgccct aaagctttat ctaaaaaata
     3661 ttttaacata gctctggtaa ttttataatc tgcaattact ccatccttta atggtttgta
     3721 ggttttgatg agttcaggtg tacgacccag catttctttg gcttcattac caattgctaa
     3781 aatttgatta ttatctaatg atacagctac aacagttggc tcattgataa caataccttt
     3841 gtttgggaca aatacaacag tattggcagt ccctaaatct attcctattt gttttataaa
     3901 cataaattta tttgaatttt ttattaaaat taatttttaa aaatatataa aatattacta
     3961 caataaatat aagaggtgaa aaatataaga agtannnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnaaagat aaaatttgac cagcataaga actaagtggt tgtcctttgt
     4141 gtatgcttaa tttcccatta acaataactg tttctatgcc ctttggggca gtaaaaggat
     4201 ttttgatatt ggctgtatct ttgatagttt ggggattaaa aataacaata tctgcatcat
     4261 tatttaattt tataatacct ttggttttga gatttaattt tttgctaggt agtcctgtac
     4321 atttataaat agcttctttg aaagataaag tagataattc tttaacatat agatcaaaaa
     4381 gtcgtggaaa agtaccaaac gctcgaggat gtatgaaatt atcagaataa tatttttctg
     4441 tgttgtgaat agtactatta gatgatacaa tacaataagg agattttaaa gccagttgaa
     4501 tattttcatt tgatacagca ttgtcatata ctaagatttg ttttggtttt aacgataaaa
     4561 tttttaaaag ggtttgttcg atagttagat taaattgttt agctacttgt tttaaagttt
     4621 taccaccgaa tacaaagtct gaactattgg ctgcaaaaaa catattggca taaagatcgt
     4681 gtttattttt tagaattttg agaagatttt tgtaaatttt ggtgtcttct aaatttttac
     4741 gcaaggtata ttcatcacca atagacaatc attctggcaa aagtaaatat aaagattcgg
     4801 ctgttaattc ataaggataa atatcaaaat taatgttggt atgaaatgtt tcattgactt
     4861 gtttgatttt tgctatagcc tttgcaaata aaggtcaatt gggcttgcca taggctttaa
     4921 aatgagtaat ttcaatattg gcattggttt ttcgagctaa atcaataact tcatcaacag
     4981 cctctaacaa tttttctgat tcactgcgta aatgtataga aactaaacca ttatattttg
     5041 taacaagttt ggacgcttct atcatttctt ttaaccctac tacttgttga tttgggtagg
     5101 ctaaaccaaa agaaaccccc atacctccgg ctaataaact ttcttttaat aaaaataaac
     5161 agctgtctaa attatctttt gttaagggct tgaattctgg cccaactaca ctttgtctaa
     5221 tagtatttca accaattaaa gttcctacat ttacaccaac ttttattttg tgtaaatatt
     5281 ttaagaattc atctacatag tttcaactaa tatttatatc ttgtgttgtt gtccaataat
     5341 tcaaaaaatc taatttacca tttactattg gcgctaatga agcaccacaa tgtccaagaa
     5401 taatagtagt aataccttgc gccagaagat tagtcgcttt gggagaattt aaaatgccta
     5461 aataatgatc tgcgctatta tttatatcaa taaacccggg gcaaacatat ttattagaag
     5521 cgttaataac atttttggca tctttatcta aaatatcatt gccaattcta ataattttac
     5581 catttttgat acctaatgaa gacataaatg catctttagc agtcccgtca aaaataattc
     5641 catttttaat taaaatatca tatttcatac aaggttttaa tgttttgata tatataaata
     5701 gcctatcata aaaatgtatt ttttgcaaca caatatttaa taagaatatt gtgttatttt
     5761 attcataaat ctataaaaaa ctaaattttg aatacaaaaa tcaaaaatat tgttaccaaa
     5821 ataggtacta aaatgtgtca gatattaaat tggtttgcaa gatttttacc agatatgctt
     5881 tctaataaat caaataatat aaaaaatcaa aatacactaa taataggata aataaaaaac
     5941 ccacctgata aattaaataa tataaatatt atttctataa aacttaaata gagaacaatt
     6001 ttggtgagaa aatctatttg tattttgtca atcaaaattt ttcaatataa aaatatggct
     6061 gatccaatag ctaagaaaaa agcaataatt cacgaggatc agtgtaattt aaaaaacaag
     6121 atatagagca acaaaataaa caaaaacaaa atagaagcaa aaaaaatatt gcgtataaaa
     6181 tcataaaaat gtggatattg ttttaattta attgcttgaa gataaaacaa taatattata
     6241 taaaacgcta agaaaataat tattgatcta gcaaaaaata aattaagtcc agctaacaac
     6301 aaaaaggtac ttagtaaaat aaaaaaatta tgtcaggcta gaccacccaa aaatttccaa
     6361 attgtcaaaa ataacaaaca taataatata tttattcaaa atggaaggct taaagcaaaa
     6421 ctacataata atagcaaaaa atataaacaa gaaaacaaaa gatgagaaaa atgaaacatt
     6481 attaaaattt attttgcctt aaatactaag ccgtctttgg atgctgtaag ataaatctgt
     6541 tttttatttt tgtattcacc tttaataatt ttgtcagcta atggatcaag tacagatttc
     6601 tggataagac gtttgagggg tctggcacca aactgaggat catatccacg taaagcaagc
     6661 cattttttgg cagaatcatc aacatttaac aaaatatttt gtattttaag acgattaact
     6721 actttttgta tttgaatatc tacaattttt tcaatatctt tgggctgcaa tggattaaat
     6781 ataataattt catcaattct attcaaaaat tcggggcgaa aagaatctct taaaacttca
     6841 ttgattttgt cttttagacg ctgtttttgt tcctcataag aattgtgctc gggcattgct
     6901 gtaatattat tgaaaccaag actggacatt tgcgaaatat attctccacc aagatttgat
     6961 gtcattataa tgattgtatt tttaaaattt actaatctgc ccttaccatc agtcaatcta
     7021 ccattatcaa gaatttgtag caaaatatta aatacttcgg ggtgagcttt ttcaatttca
     7081 tcaaataata ttaaactata aggtctatgt ctaatagttt ctgttaattg gcccccttct
     7141 tcataaccaa catatcctgg aggtgaacca attaaacgag caacgctgtg tttttccata
     7201 tattcagaca tatctatacg aattaaagat ttttcgtcat caaacataaa ttctgctaaa
     7261 gctttagcga gttctgtttt accaacacct gttgggccca aaaacataaa tgatccaatg
     7321 ggtttatctt cttcagaaag tccagcgcga gaacgacgaa tagcacgaga aacagcagaa
     7381 atagcttcgt cttgaccaat aactcttgat gataaaatat tctccatatt ggcgagtttg
     7441 tttttttcat tttcgagcat acgttcaacc ggaataccag tccattgaga aacacattta
     7501 gcaatttcct cactagtaac ttcttcttga ataaaacttg aagaattttt cttttttgtt
     7561 ttgtgttcta ttttagcaat ttcttttttg agcataggaa ttttaccata aataatttcg
     7621 gcaactttgt ctaaactgcc ttgtttttca aaattttgag cctctctttc taaattttca
     7681 atctgttgtt taagttcatg gattttatta tgtggctctt tttcactatt tcattgttgt
     7741 aataatttgt cttcttcttt ttttaaagtt tctaattttt tatttactac tgacaaacgt
     7801 ttttgggatt ctttatcttc ttctttttta agagcttctt tttctatttc taatgtacga
     7861 atttctttct ggatattagc tatagttggc ggtacactgt tcatttgaag ttttagatga
     7921 gatgcagctt catcaataag atcaatggct ttgtctggta aaaatctatc gggaatatag
     7981 cgaactgaca aatttacagc atctcgtata gctgaatcag taatttttac tccatggaat
     8041 acttcatatt taggttttaa tcctctgagt attgtaatag tatcttctat acttggttcg
     8101 tcaatgataa cgggctgaaa acgtctttct aatgcaggat ctttttctac tgaattacga
     8161 tagtctttat atgttgtggc accaatcaaa tgtagttcgc ctcgagccaa agctggtttt
     8221 aataaattag aggcatcaat agatccttcg gcagcaccaa caccaacaat agtttgtagt
     8281 tcgtcaataa aaataatgta tttaccttcg ctggctttta tttcttttaa aacactcttt
     8341 aatctatctt caaactcccc gcgaaatttt gttcctgcta ctaatgcacc aatatccaaa
     8401 gatattattt ctttgttttt taaattatca ggaacttcat tttcaataat tttttgtgct
     8461 aaaccctcga caatagcggt tttacctact cctggttcac caagtaaaac aggattattt
     8521 ttagtacgtc gagataaaat ttgcataata tgataaagct cttcttctct tccaataaga
     8581 ggatcaagtt tgtgagctct ggctaattct gtaagatttt gactatattt ttctaaggct
     8641 ttgtactgag cattgccatg ttcatctaac tcttgtcctt tgtttttaaa actatttaat
     8701 atgtttcaaa ttacatcagt gttaatttta tatttctcca aaagagtttt ggcactagaa
     8761 tttacagtgc ttatagctaa taatatatgc acgggggaaa tatacttaga atttgtttct
     8821 ttggaatatc tagcagattt ttccaaaacc attattactt cttgatccaa agctatttgc
     8881 ccaaaatttt gcattttaaa agcattagct atttgttcaa gttgtttttg aatatcatta
     8941 ataattgtgt ctttgtttag ttttagttct gacactacaa catcaaaaaa actattatct
     9001 tgttcagcaa tagctaatag taaatgtaat acttttattt cttcgtgatt atattctaca
     9061 gcgagctgtt gagctcgttc aataacatct tgcgcttctg gattaaattg tggaattgta
     9121 ggcatattaa ttagtttaat aattactaaa tttgagttta tactctgtta aaattgatta
     9181 aaaaaatttc taacaaggta tagtataata tataaatttt ttatcaattt gtttattttc
     9241 tatttagtag taatccacgc taatttacat aaacctaaga taaatctaaa ttttgatcta
     9301 tgtatattca cgcgttttag tctgtagaat ctttactacc taaaatcaaa tctaaataaa
     9361 ttgttttctt atcacgtagt acttctaatt taactttatc accgatatta aaacggcgaa
     9421 caatagaaga taatgaattc tgagctgtaa tttttatatt atttattgat aaaataatat
     9481 cattttcttt caaaccagct ttttcagcag gagatccttt aactatagct ggatcattat
     9541 ttgcgccctt tccaattaaa gcaccataat caacaggaag attcttttct ttcattaaat
     9601 ctttatcaat tatagtataa tgaataccaa taaaaggaac agatattttt cctgtggaaa
     9661 ttgcttgatt aataattccc tttatcttac taataggaat agcaaatcca atattttgtg
     9721 caccttgctc cattgctaca ttaacaccta taacttctcc atttaaattt aataatggtc
     9781 cgccagaatt accctggtta atagcggcat ctgtttgaat taaatctgtt aaagtagtgg
     9841 gctcaccagt agtatcataa gctgtaatat ttctatgtaa tcctgaaacc acaccaacac
     9901 taactgtatt ttgtagttca cccagagcat taccaatggc aatagccgtt tgtccaagca
     9961 ttaaattgtc agaattacct aatattagtg gtactaaccc agttttatta attttaacta
    10021 aagcaaaatc ttctaaagga tctctggcaa tgactgtagc gctatattca gtaccatcat
    10081 ttaacaaaac cgtatattca gcttttgtat cttgtacaac atgtttgtta gtaataatat
    10141 aaccatttga atcaattata aaaccactac cagcacttac ttcttgtttt tttgtcccct
    10201 cttgcttttg ataatattga ggaatattaa aaggaaaccc aaaaaaatca ttaaacggat
    10261 cagatgggtt aatagggatt tgataagttt taataattgg taaatcttta cttgcaacaa
    10321 tactaactac agatttttcg gcttttttaa cactatttat tactctttgt tcgtgaacat
    10381 cttgactttg ataaggaact gacgattcta cattcttaat agtttgaata gtggatgtat
    10441 tagttgaagt agattctttg ctaaatggtc aaagtaatat attctgtcaa ttatatgcag
    10501 acactgaatg agcagcaata ccaatagttg ttataataat tactaaaata atacaaattc
    10561 aaatagactt tgatattttt gtatgtttca taatattcag tttatataat ttattaaaaa
    10621 ataactaata aattattatt tatttttctt cgtttgtatt tgttggttca gaattattat
    10681 ttaaaattac agggcgagat gaaatatctt tgaaaagaac agtataaaaa atagggaaaa
    10741 atacaagtat aataagaata tttaaaatag atgtttggat atacaaagaa ataataaata
    10801 caacagcaaa aacaactcaa aaaataataa aaggaatgag cgtttctttt caatgagtaa
    10861 tatttagatg acaactttgc tgaatggttt ttataggttt tgatttttta atagcagtaa
    10921 ttggatatac taatgataaa agaaagagag caaaaacgcc tggtgctata aatagaataa
    10981 aacctatatt aatcaacagt atacatagta ctgtgagtaa aaatgcatta ctaaataaat
    11041 gccaagattt ttgaaaagct tttttccaag atttttcacc attggcgact tcaatagaag
    11101 caattataca aaaagtaaat atagcgagta ccagaacgcc acatattgta aaagtataaa
    11161 taaaaagtgg atttctcatt atacttaata taaaggcttc gtcagttgaa atgtttgacg
    11221 tcatatttat atttagtttc gaactaacaa gcgccaaagg aacaaaacat atagaggaaa
    11281 gaataaattg aaacaaacaa ataattaaaa aggaaactca gttatcttga aaaatttttc
    11341 acgaatcctt ggtatattga ataaatttat tcataattgt tgattaaata ttttattaaa
    11401 gatttagtaa atatttttat tttgttgtat tttgagtcga attattaact ggggtattag
    11461 ctgttggagt agctggcgaa gataatggtg tagatgattg tgaagattgt atagttgggg
    11521 cacttgcagt aacagaaaca ctggaattat tgttagcaat accatcaata gacaatttaa
    11581 ctttatcttt aactttattt actaattcat ctactgataa acgcgccttg acaatataat
    11641 caatgacacc caatgattta cctctggcaa tatcagaatc ttgaccgagc attgaaatta
    11701 taataacagg aatattagca aagcgcggat cttgtttcat tgattccaat acttcaaaac
    11761 catttttctt aggtaatatc aaatcaagaa gtaccaaatc aggcataaaa gtttctaaaa
    11821 tttgtattgc ttcttcgcca tctttggcac gttctatttg aaacccttct tgttcaagtt
    11881 tcatttgtaa tagactactt aaaaacaatt cgtcttctac taataaaatt ttgtgtttat
    11941 tcatatgtaa aattaaatat taaaaatata aattaaaccc aagataaaag aaacaaattg
    12001 tatttatact tgttcctatt tatatatggt attatatatt attatagcat ttattttaga
    12061 aaaatgcaat acctttggtt taaacaaata atattgttta actcaaaaat cccaatttta
    12121 tataattatt ttatcgggaa tatatattca acacatttag atcctttagc tgtctcaaat
    12181 ctatcgtttt aaaaatttaa gatgaaaaat caaactataa tggtttgatg tgaccttgga
    12241 atattttgat tataataata ttaaaatact attaaatcaa aaaataaatt cctaaaagag
    12301 atgctccact cccaccttct ggtaggcgta ccttgttacg acttctcccc tgtcatcgaa
    12361 cctagcttac cccctccttt gaccattagg ttttaaagaa acaattaaca aaattaacaa
    12421 ttaaaattag taacttaaaa tatatttaac tgttaaatgt ttaaagttta tttacctatt
    12481 aggcaaagga gggggttcgg ctatttccga ctcccttgaa gtgacgggcg gtgagtacaa
    12541 agctcgggaa catattcacc atggtatggc tgacccacga ttactagcga ttccaacttc
    12601 aaaaggtcga gttgcagacc tttatctgta ctggggaatg ttttttggga ttagctccat
    12661 cttacgactt ggcaaccctt tgtgcattcc attgtagcat gagtgtcgcc cagggtatca
    12721 gagggccatg ctgatttatc gtcatcctca ccttcctccc cctcctttga ccattaggtt
    12781 ttaaagaaac aattaacaaa attaacaatt aa
//
LOCUS       DAOTJA010000013        12321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_5576_length_12321_cov_5.757623, whole genome shotgun sequence.
ACCESSION   DAOTJA010000013 DAOTJA010000000
VERSION     DAOTJA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5576_length_12321_cov_5.757623"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="P5241_01500"
     CDS             complement(<1..1246)
                     /locus_tag="P5241_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32231.1"
                     /translation="MGEKSFKKLTQDEIKKIKLSYCNRSETGITVEKLAILLANEFNV
                     SERTVRKWFKKLGLKEKTEIEPEQYEEAKKRELNKKKKVYFFTWAQNNTPPHTAFLNN
                     MKAYAKFRGADIHIIAGRYKNPTSINTDEKYDFWHEEITPYLDANRHEVSKGLVVLSD
                     VKIQPTAVNPLSGMAGMSGINSCIFGSPKVHFEVIPAMEGYTPKKMWTTGACTVKNYT
                     DSKSGKKGEFHHTLGFVIVEIEDDETFHVRQVTANNNGEFCDLIYEVKNGQVSKIDGV
                     EAIVLGDIHIGDLDESVFKTTKKMLDKLNPKHTIIHDLFNGHSITHHDLKNPIKLYYK
                     EIDGTNSLKREINEMITWLESMRKYNLVIVRSNHDDFIDRWIMNADWKKDIKNSMEYM
                     EYAKILLENKAPKGIIPYIIDSK"
     gene            complement(1239..2138)
                     /locus_tag="P5241_01505"
     CDS             complement(1239..2138)
                     /locus_tag="P5241_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32232.1"
                     /translation="MKKTLKFIEKAKKIHGDKYDYSNTIYTESKNKVNIICPIHGIFE
                     QRASGHLSGYGCQKCSADKQRLGCDEFIKRANEKHNNKYDYSLVEYKTYNDNVTIICP
                     IHGKFEQSPAHHLNGIGCLECSFIHRRIGTEKFIRKANEVHGNKYDYSKVEYKKAIIK
                     VEIICPKHGEFKQTPHNHLKGNGCPYCRESKGEEKIRMCLENNNIKYFQEYKFSNCKD
                     VKSLLFDFYLPDYNTCIEFNGIQHYKSIEYFGGKNALKKQQKKDKIKELFCKKNNINL
                     IIVKYNEKLEKKIINFINKCKNG"
     gene            complement(2347..3108)
                     /locus_tag="P5241_01510"
     CDS             complement(2347..3108)
                     /locus_tag="P5241_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HRV32233.1"
                     /translation="MKKALDVRMKKYEKIYSFQVPPKTYTIIRFDGKNFSKYTSKFTK
                     PFDNILSNAMNDAAIAVCKEFNPRLAYTQSDEISILLTDIYNIESELPYDGKIQKLCS
                     IGASIVSNEFNRSMMIALVKSPLEIANFNFAKFDARVFIIPDIREVYNYFVWRQQDAT
                     RNSINMAAHAAFGPKAIKYKNTNELQEMLYQNKGINWNDYPEKFKRGCVIKRETYFKE
                     TEDNKKLIRNKWAIQATPIFTQEKEFLQNIIVPVL"
     gene            3218..3958
                     /locus_tag="P5241_01515"
     CDS             3218..3958
                     /locus_tag="P5241_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32234.1"
                     /translation="MSNIYIKNYTFMRNFIRSLLKKLIIESDYFTVINPIDGSEPHNV
                     TYDDLKKITYKVKNAQEKYGHIKYFQDAKEGDGTYLAIINKNGLVTIKTPNHYIDDKE
                     IGGVSTTRAYDNLEIPIRAYSGDDDLLQTGRAKIGLQLQKPYTKSGAADARIKVYVIF
                     GDMIIDRVKDNVKGHNDADDYKDNTYQGRTDKQIARKDFIDQREKERAARKEKRSPIT
                     AFSSEKEKQDYLDKQEALRLKYAKFRKK"
     gene            complement(4546..4689)
                     /locus_tag="P5241_01520"
     CDS             complement(4546..4689)
                     /locus_tag="P5241_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32235.1"
                     /translation="MKKCKICGNKTEVVFNIDFNAVPICEDCVTAIFIQQAKWYAEQD
                     KKK"
     gene            complement(4765..5133)
                     /locus_tag="P5241_01525"
     CDS             complement(4765..5133)
                     /locus_tag="P5241_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32236.1"
                     /translation="MSLIKFKLTEDHIKLVRQLKINIRNNDENIDYIIIDNKEPFGSK
                     DIFDDIFLILYGKPSNGELNINPWDKGQKPWTDEQLNYMKKLLIELPYALDIILYTGK
                     FEVGFYKTKSYHRDWKKINE"
     gene            complement(5169..5597)
                     /locus_tag="P5241_01530"
     CDS             complement(5169..5597)
                     /locus_tag="P5241_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32237.1"
                     /translation="MSIIPEVKKKGFFSCKDSGKISDNMIENFKYNNHEYKLIYKSMD
                     PLKKEMVVVEPEIAKRAIKEFIENEIGHYIDDEFKLINKSINDKVEEVKTSLKTYIDN
                     KVDMIAEQIVTELISTNFKEAVNKKIDEKLNRIKDLLIDE"
     gene            complement(5700..6287)
                     /locus_tag="P5241_01535"
     CDS             complement(5700..6287)
                     /locus_tag="P5241_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32238.1"
                     /translation="MKEMVIYEKLDNLYRNENSKKFIDHLIKSYIPKSKIEKVVEKPK
                     GRFRCAITNVKLVAIEDVFKEIGEDKEKQNQFIEHVSSLLDDSVKPAPKLDIFKTNSL
                     ALTAKSTDTFLSQEAFHIFFKWIQKKMLEGDKHIYWVIKQMQAEEEFFIKRVKINDEE
                     KIKFINKKQKVKATFGDLTVLQELKKHLKHKKDND"
     gene            complement(6738..7082)
                     /locus_tag="P5241_01540"
     CDS             complement(6738..7082)
                     /locus_tag="P5241_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HRV32239.1"
                     /translation="MNIAIVGSRTFNNYELMNKILSNYLPNIDAIVSGGARGADLLGE
                     RFANENGISTIIFKPDWKKYKKSAGFIRNRLIVENADIIIVFWDGISRGTAHTIELAK
                     KMNKKIIVIEFS"
     gene            complement(7079..7564)
                     /locus_tag="P5241_01545"
     CDS             complement(7079..7564)
                     /locus_tag="P5241_01545"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRV32240.1"
                     /translation="MKCPNISIVVAASENNCIGKNNKIPWHIPTDLYRFKKLTDGHIV
                     LMGRKTYESIGKPLRDRINIVASRKPYISQHFNVLIDDDVWSNIHQWRDEIFIIGGSQ
                     IYEEGFKYANTLYLTRIHAHIDGDAFLKGFNEDEWELIKEEGPLEENGFKFTFLTYKR
                     K"
     gene            complement(7661..7960)
                     /locus_tag="P5241_01550"
     CDS             complement(7661..7960)
                     /locus_tag="P5241_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32241.1"
                     /translation="MKNKCELCEREVLTDEHHLIPKTNHNNKWFLKHFTKDEMNNKKI
                     NVCYDCHLAIHKFIPDEKELGKSYNTKELLLSHPKIYKFVEWIKKQSGNISKTKT"
     gene            complement(7976..8485)
                     /locus_tag="P5241_01555"
     CDS             complement(7976..8485)
                     /locus_tag="P5241_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32242.1"
                     /translation="MEKELNFFNFESDVLLRKWEFLGLLNGLSKRSSRIIARNFEYAA
                     NEVGFIIDNPLTKYEVAKKFPLPERFGNFVEILFPMIRRIMELILVDDDDVIFSKNHT
                     KKRIPQALINLNEKHIIRARDIYYILIETIKNTPQDEVGLLGFDYEAKIIVKSYENYI
                     LKFGMNSYS"
     gene            complement(8549..9169)
                     /gene="coaE"
                     /locus_tag="P5241_01560"
     CDS             complement(8549..9169)
                     /gene="coaE"
                     /locus_tag="P5241_01560"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRV32243.1"
                     /translation="MLKIGITGPIGAGKSYISKLFADMYGIPVFDSDSVAKELQLNNE
                     FVKSRIINLIGSEAYNIDGSVNCKYVADIIFNEPNKNEIIFDIIRGPLIARMYEDYYS
                     YKYMRPNRPPFMLIENAIMIQEKAFHLMDNIILVNASYEVRMERVIKRGMKEEDFIRR
                     NAKQMQINEMQDILNRHGVPSFMIVNEYDIYIPSIVEFYYKMITKK"
     gene            complement(9420..10016)
                     /locus_tag="P5241_01565"
     CDS             complement(9420..10016)
                     /locus_tag="P5241_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32244.1"
                     /translation="MKSNFIIGVDVDGVLRDMDAVMMDIFKELYPEHVKSDIVYGWDF
                     PNVELPQKRKMDIMFKEYPKEVFENSLPFEGVHDEYLKLNAWSIENGGKLACVTNQKP
                     KLIHHTLIWLGKHKFNFDEIYFTKEKEDLAIDFLIDDSPKNFERWVKAGRDPKRYIIM
                     DRDYNHDINATNRISRLSEAIQIIKFLMLKDKLIKKIK"
     gene            complement(10482..10562)
                     /locus_tag="P5241_01570"
     tRNA            complement(10482..10562)
                     /locus_tag="P5241_01570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10518..10520),aa:Gly,seq:gcc)
     gene            complement(10917..10989)
                     /locus_tag="P5241_01575"
     tRNA            complement(10917..10989)
                     /locus_tag="P5241_01575"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10954..10956),aa:Lys,seq:ttt)
     gene            complement(11116..11205)
                     /locus_tag="P5241_01580"
     tRNA            complement(11116..11205)
                     /locus_tag="P5241_01580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11169..11171),aa:Glu,seq:ttc)
     gene            complement(11354..11440)
                     /locus_tag="P5241_01585"
     tRNA            complement(11354..11440)
                     /locus_tag="P5241_01585"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11404..11406),aa:Glu,seq:ttc)
     gene            complement(11800..12153)
                     /locus_tag="P5241_01590"
     CDS             complement(11800..12153)
                     /locus_tag="P5241_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRV32245.1"
                     /translation="MNPQMLIKCTVLALHITTLYDLSKRTISKILKRMKLPCSKCGWY
                     VEGVVGDIHHILERKNGGTDEHNNLTYVCPNCHRLIHTNKIKISELVNLEDFIGDTWK
                     KFYYVKNGKLEKLNN"
     gene            complement(12131..>12321)
                     /locus_tag="P5241_01595"
     CDS             complement(12131..>12321)
                     /locus_tag="P5241_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRV32246.1"
                     /translation="FIKASSNEGDLVADFYSGSFTTAEVCKDLKRNFIGCDINPNCLE
                     KAKERGLFSFTDEPTNVD"
BASE COUNT         4118 a         2018 c         1383 g         4802 t
ORIGIN      
        1 attttgaatc aataatatat gggataattc ctttaggtgc tttattctct aacaatattt
       61 tagcgtattc catatattcc attgaatttt taatgtcctt tttccaatcg gcattcataa
      121 tccaacggtc aatgaaatca tcatggttgc ttcttactat cactaaatta tatttacgca
      181 tactttctaa ccatgtaatc atttcattta tttcacgttt taatgaatta gttccgtcaa
      241 tttccttata atataattta attggatttt ttaaatcatg atgggtaatt gaatgaccat
      301 taaaaaggtc atgaattata gtatgttttg gatttaattt atctagcatt tttttagttg
      361 ttttgaaaac agactcatct aaatcaccta tatgtatatc acctaaaact attgcttcaa
      421 caccatcaat tttggaaacc tgtccatttt taacttcata aattaaatca caaaactccc
      481 cattattatt agcggttact tgccttacat gaaatgtttc atcatcttct atttcaacaa
      541 taacaaatcc taatgtatga tgaaattcac cctttttacc agatttagaa tctgtataat
      601 ttttaaccgt acaagcacct gtagtccaca tttttttcgg agtatagcct tccatggctg
      661 gaataacctc aaaatgaacc tttggggaac caaaaataca agaattaatt ccactcatgc
      721 cagccattcc gcttaatgga ttaacagctg taggttgtat tttaacatcg gataacacca
      781 ccaatccttt actaacttca tgtctatttg catctagata aggtgttatt tcttcatgcc
      841 agaaatcata tttctcatct gtatttatgg atgtgggatt tttatatctt ccagcaataa
      901 tatgaatatc agcacctcta aatttagcat atgctttcat attatttaaa aaagcagtat
      961 gaggtggggt gttattctga gcccatgtaa agaaatatac ttttttcttt ttattcaatt
     1021 ctcttttttt agcctcttcg tattgttctg gctcaatctc cgttttttct tttaatccta
     1081 attttttaaa ccattttcta acagtccttt cagaaacatt aaattcatta gctaggagaa
     1141 tggctaattt ttcaactgta attccagttt ctgaacgatt gcaataagat aattttattt
     1201 tttttatttc atcttgtgtt aattttttaa aacttttttc acccattttt acatttgttt
     1261 atgaagttaa ttattttttt ttctaatttt tcattatatt taacaatgat taaatttata
     1321 ttattttttt tacaaaatag ttcttttatc ttatcttttt tttgttgctt ttttaatgca
     1381 tttttaccac caaaatattc aattgattta taatgttgaa ttccattgaa ttcaatacaa
     1441 gtattataat ctggtaaata aaaatcaaat aataatgatt taacgtcttt acaattacta
     1501 aacttatatt cttgaaaata tttaatatta ttattttcta aacacatcct aattttttcc
     1561 tcacctttgg attctctaca atatgggcag ccattacctt ttaaatggtt atgtggtgtt
     1621 tgtttaaatt caccatgttt agggcatata atttcaactt taataattgc ttttttatat
     1681 tccacttttg aataatcata tttattacca tgaacctcat ttgcttttcg aataaatttt
     1741 tctgttccta ttcttctatg tataaaacta cattctaaac aacctatacc atttaaatgg
     1801 tgtgctggcg attgttcaaa tttaccatga attggacaaa ttattgtaac attatcatta
     1861 taagttttat attctaccaa tgaataatca tatttattat tgtgtttttc gttagctctt
     1921 ttaataaatt catcacagcc caatctttgc ttatccgcag aacatttttg gcatccataa
     1981 ccacttaaat gaccgctagc cctttgctca aaaattccat gaataggaca tataatgtta
     2041 actttatttt tactttcagt atatatagta ttactgtaat catatttatc accatggatt
     2101 ttttttgcct tttctataaa ttttaatgtt tttttcataa tatcttttat tatatatatt
     2161 atgaaaaatt aaaagttaaa atattttctt gtagtacgtt ctgattttcc aaaatgttta
     2221 gttagaatgt tcattctctc atcccaactc atgtttttgt ttaaataaat ttgtttagcg
     2281 tactctattt cattagaggt taaattttta aatttcatta cgaataatta aaactaatat
     2341 tatttattat aatacaggaa caattatatt ttgtaaaaat tctttttcct gtgtaaatat
     2401 aggtgtggct tgtattgccc atttatttct tattaatttt ttattatctt cagtttcctt
     2461 aaaataagtt tcacgtttga ttacacaacc tcttttaaat ttttcaggat aatcattcca
     2521 attaatacct ttattttgat aaagcatctc ttgaagctca ttagtatttt tgtatttaat
     2581 tgccttcggt ccaaatgctg catgtgctgc catgttaata gaattccttg ttgcgtcttg
     2641 ttgtctccac acaaaataat tataaacttc tcgaatatca ggaattataa ataccctagc
     2701 atcaaatttg gcaaaattaa aatttgcaat ttctagtggc gatttaacta atgcaatcat
     2761 catggaccta ttaaattcat ttgaaactat actagctcca attgaacata acttttgaat
     2821 ttttccatca taaggcaatt cactttctat attgtaaata tcggtcaaaa ggatagaaat
     2881 ttcatcagat tgtgtatatg ctaatctcgg attaaactct ttacataccg ctattgctgc
     2941 atcgttcata gcattggata atatattgtc aaacggttta gtgaatttac ttgtgtattt
     3001 agaaaaattt ttgccatcaa acctaataat tgtgtaagtt tttggtggaa cttgaaaaga
     3061 ataaattttt tcatattttt tcattctcac atccaatgct tttttcataa tcttttaata
     3121 aattctaaca caaaattact acatttttta taaattgtca agaaaaatac tcttttattt
     3181 attttattaa taataaaatt aaggcacttt ataataaatg tcaaatattt atattaaaaa
     3241 ttatactttc atgagaaatt ttattcgtag tttactaaaa aaactaatca tagaaagcga
     3301 ctattttact gttattaatc caattgatgg ttctgaaccg cataatgtta cttatgatga
     3361 tttaaaaaaa attacatata aggtaaaaaa tgcacaagaa aaatatgggc acataaaata
     3421 ttttcaagac gctaaagaag gtgatggtac ctatttagct attattaata aaaacggatt
     3481 agtaacaatt aaaactccta atcactatat tgatgataaa gaaatcggag gtgtttctac
     3541 aactagagca tacgataatc ttgaaatacc tatcagagca tattcaggtg atgatgattt
     3601 attacaaact ggtcgtgcaa aaataggttt acaattacaa aaaccatata caaaaagtgg
     3661 ggcagctgat gcacgtatta aagtatatgt gatttttggc gatatgatta ttgatagagt
     3721 aaaagataat gttaaaggac ataatgatgc tgatgactac aaagataata cataccaagg
     3781 tagaactgat aaacaaatag caagaaaaga ctttatcgac cagcgtgaaa aagaaagagc
     3841 tgcaagaaaa gaaaaacgaa gtccaataac tgcttttagc tcagaaaaag aaaaacaaga
     3901 ttatttagat aaacaagagg ctttaagact taagtatgca aaatttagaa aaaaataaat
     3961 ttgaaattac acaaatcttt gtttatttaa ttcatacgct ttagctaatg tttcatagtt
     4021 attcacattt ttacagtttc ttagtgcttg ttccatcata tacatttttt ggtatttaac
     4081 acacagaaac attatctctt ccataattta gtttttttta tattgtaaag caaaaataat
     4141 atatcttata acaaaaaaaa aatacaatat attaatttat acacaaatca cgttaacagt
     4201 aatattaaat gggttcatag tgtttattac ttacctcctg atttatatca ataaataaag
     4261 cgactaaaga atattcaaaa aaagattcat ttccccactt ttcactattc gctacactta
     4321 ctactaaaat ttttaaaatt atcaaacatc ccaattggaa caattatttt tctattatca
     4381 accgtatatt gcttgaacaa gagtattcct cactgccatt gattataata tctttcaaac
     4441 attacaatgt cattccattg cgaatatttt ggtccgttgc aattaaaatt ttatctctct
     4501 aaagtagata aagttttttt gtaatttcat tttttatcat tataattatt tttttttatc
     4561 ttgttctgca taccatttag cttgctgaat aaaaattgca gtgacacaat cttcacatat
     4621 aggtacggca ttaaaatcta tattgaaaac aacttcggtt ttattaccac aaattttaca
     4681 ttttttcata ttttgattat ttttatactc ccataaactt tttttatatt atagttataa
     4741 acttaattta taatgatttt tcaatcattc attaattttt ttccagtctc tatgatacga
     4801 tttagtttta taaaatccaa cttcaaattt cccagtgtat aaaataatat ccaatgcata
     4861 tggaagctca attaaaagct ttttcatata atttagttgt tcatcagtcc acggtttttg
     4921 ccctttatcc catggattta tatttaattc tccattactc ggtttgccat acagaataag
     4981 aaatatatca tcaaatatgt ctttacttcc gaatggttcc ttattgtcaa ttataatata
     5041 atcaatattt tcatcattat ttctaatgtt aatttttaat tgacgaacca acttaatatg
     5101 gtcttcagtt aatttaaatt taattaaact catactcttc aatttaattc tttcgttatt
     5161 ttattaactt attcatcaat taataaatct tttatacgat ttagtttttc atctattttt
     5221 ttgttaacag cttctttgaa attagtagaa atcaattcag ttacaatttg ttcagcaatc
     5281 atatcaactt tattatcaat atacgtttta agtgaagttt taacttcttc aactttatca
     5341 ttaattgact tattaattaa tttgaattca tcatcaatat aatgaccgat ttcattttca
     5401 ataaattctt ttattgcacg ttttgctatt tctggttcta caaccaccat ttcctttttt
     5461 aacgggtcca tacttttata tattaactta tattcatgat tattatactt aaagttttca
     5521 atcatattat cagatatttt tcctgagtct ttacatgaaa aaaaaccttt ttttttaact
     5581 tctggtatta tagacataca ttttttttac aaaaatatgg aaaaaaagaa aaaaaagcaa
     5641 ctttttttat ttaatttatt aaattatgat gaattttcta aagatatatc ataaaaaagt
     5701 taatcattat cttttttatg ctttaaatgt tttttaagtt cttgtaaaac cgttaaatcg
     5761 ccaaatgtag ctttaacttt ctgtttctta tttatgaatt taattttttc ttcatcatta
     5821 atcttcaccc gttttatgaa aaattcttct tcagcttgca tttgtttaat gacccaataa
     5881 atatgtttat caccttccaa catttttttc tgaatccact tgaaaaaaat atgaaatgct
     5941 tcttgagata aaaaagtatc agtactttta gccgttaaag ctaaactatt agttttaaat
     6001 atatcaagtt taggggctgg tttaaccgaa tcatcaagaa gacttgaaac atgctcgatg
     6061 aattgatttt gtttttcttt atcttcacca atttctttaa aaacatcttc gattgcaact
     6121 aattttacat ttgtaattgc acacctgaaa cgacctttag gtttttctac tactttttct
     6181 atcttacttt ttggtatata tgattttatt aaatggtcaa taaatttctt agaattttca
     6241 ttcctataaa gattatccaa cttttcataa atcaccattt ctttcatcat catactacaa
     6301 cattatttaa aaaaattaaa acttattaac aaaataatta catgtgccaa ttggtcaatt
     6361 ccaatcacat tgaaaaatgc tctattcatt ttctttaaat agaaataagt ttcaatttta
     6421 ctcgttgtaa aatctattag aaaatgaaat attgcattcc ccaaagcaaa taagaacaaa
     6481 taaaaataag aagaaattac aataaaggaa agaatgttgc acaaaaaaag taaaaaaaat
     6541 atacctacac cataaataaa acagtgtgtt ccaagccaat aaatattttc ggatttttta
     6601 ctagccatta tttcagtttg catgataaag tctgcaatat aatgaacaat tagaattata
     6661 aaaattgtct ttaaatccat ataaccttaa tttttataca aaaacatatt ttttttggaa
     6721 attttacaag ttttaaccta agaaaattct ataacaataa ttttcttatt cattttctta
     6781 gccaattcaa ttgtatgagc agttccacga gaaatcccat cccaaaacac aataataata
     6841 tcagcattct caacaattaa tctgtttcta ataaatccag ctgacttttt atattttttc
     6901 caatcaggtt taaaaataat ggttgatatc ccattttcat ttgcaaatct ttcacctaat
     6961 aaatcagcac ctcttgcacc accagataca attgcatcaa tattaggtaa ataattactt
     7021 aaaattttat tcattaattc atagttattg aatgttctac taccaacaat tgcaatattc
     7081 attttcgttt atatgttaaa aaagtaaatt taaaaccatt ttcttctaat ggaccttctt
     7141 cttttatcaa ttcccattca tcttcattaa aaccttttaa aaatgcatca ccatcgatat
     7201 gtgcatgaat tctagtaaga tataatgtat tagcatattt aaacccttct tcatatattt
     7261 gagaaccacc gattataaaa atttcatctc tccattggtg gatattagac cagacatcat
     7321 cgtcaattaa aacattgaaa tgttgagaaa tatatggctt tctacttgca acaatgttaa
     7381 tcctatctcg taatggtttt ccaattgatt cataggtctt ccttcccatt aggacaatat
     7441 gcccatcagt taattttttg aatctatata aatccgttgg aatatgccat ggaatcttat
     7501 tgttctttcc aatacaatta ttctcactcg ctgctactac tatcgaaata ttagggcatt
     7561 tcatattatt tcatgaaatt ttacagttat ttatgttttt cctataaata ttatcaaata
     7621 ctactatatt taatcaataa taaaaaaaat aagtaatata ttatgtctta gtttttgaaa
     7681 tattaccact ttgtttttta atccattcaa caaatttata aattttagga tgggataata
     7741 aaagttcttt tgtgttatat gattttccaa gttctttttc atctggaata aatttatgaa
     7801 tagctaaatg acaatcataa catacattaa ttttcttgtt attcatttca tcctttgtga
     7861 aatgtttcaa gaaccattta ttattatgat ttgtctttgg tattaaatga tgttcatcag
     7921 ttaaaacttc tcgttcacat aattcgcatt tatttttcat atcaaatatt ttttattaag
     7981 aatatgaatt cattccaaat tttaatatat aattttcata agattttaca attatcttag
     8041 cctcataatc aaatcctaac aaacctactt catcttgcgg tgtattttta atggtttcaa
     8101 ttaaaatata gtaaatatct ctagccctta taatatgctt ttcattcaaa tttataagtg
     8161 cttgaggaat tctttttttt gtatgattct tagagaaaat tacatcatca tcatctacca
     8221 aaatcaattc cataattctt ctaatcatag gaaataaaat ttcaacaaaa ttaccaaatc
     8281 tttcgggtaa tggaaatttt ttagcaacct catatttggt taatggatta tcgatgataa
     8341 atccaacttc gttagcagca tattcaaaat tacgagcaat tattcgtgag cttctttttg
     8401 aaagtccatt aagtaaacct aaaaattccc attttcttaa taatacatca ctctcaaaat
     8461 taaaaaaatt aagttctttt tccatagtta agtgaattat ctataacatg ttattataca
     8521 acattcaaaa tatcttatta aattttattt atttcttagt aatcatttta taataaaact
     8581 ctactatact aggaatatat atatcatatt cattgactat cataaatgaa ggaacaccat
     8641 gcctatttag aatatcttgc atttcattta tttgcatttg tttggcatta cgtcttataa
     8701 aatcttcttc tttcattcca cgtttaatta ctctctccat tctaacttca taacttgcat
     8761 taaccaaaat tatattatcc attaaatgaa atgctttttc ttgtatcata attgcatttt
     8821 caatcaacat aaacggcggt ctattaggtc tcatatactt gtaactgtag taatcttcat
     8881 acatcctagc tattaacgga ccgcgaataa tatcaaaaat tatttcattt ttattaggct
     8941 cattaaaaat aatatcagca acatacttac aatttactga accatcaata ttatatgctt
     9001 ccgaaccaat cagattaatt attcttgatt taacaaactc attattcaat tgcaattctt
     9061 ttgctacaga atctgaatca aatactggaa ttccatacat atcagcaaat aatttgctga
     9121 tgtatgactt tccagcccca ataggtccag ttattcctat ttttaacata atactttatc
     9181 ttttaataat ttttaaatca caccaatatt gacttatttg aaaataatct tatttagcca
     9241 aaataagtat tttcagcaac caaatataat aaaaaaattc cagaaaagca aaattttttt
     9301 ctggaatttt ttaaaaatat ttttaagtaa attaatttaa tttttcataa ttactatgtt
     9361 taatatattg ggaattttta ataaattatt tctattttga tgaaattttt cttaaacaat
     9421 tattttattt ttttaattaa cttgtctttt aacatcaaaa atttaataat ttgaattgct
     9481 tctgatagtc tactaattct atttgtagca tttatatcat gattataatc cctatccatt
     9541 atgatatatc tctttgggtc tctacccgct ttaacccatc gttcaaaatt ttttggtgaa
     9601 tcatcaatta aaaaatcaat tgctaaatct tccttttctt ttgtaaaata tatttcatca
     9661 aaattaaact tatgttttcc taaccagatt aacgtatggt gaattaattt tggtttttga
     9721 ttggtaacac aagcaagttt accaccattt tcaatagacc aagcatttaa ttttaaatat
     9781 tcatcatgaa caccttcaaa tggtaatgaa ttttcaaata cttcttttgg atactcttta
     9841 aacataatat ccattttacg tttttgtggt aactcaacat taggaaaatc ccaaccataa
     9901 acaatatccg atttaacatg ctctggataa agttctttaa atatatccat cattacagca
     9961 tccatgtcgc gtaatacacc gtcaacatca acaccaatga taaaatttga tttcatgttt
    10021 aaacaattta ttaaataatt ctattttaaa tattcttcta tggttggttc acctatattc
    10081 atttttttac tttctcaata tcttttttaa tttcttcagg tggtgtttct ttatagtatt
    10141 tctctacatt tttagccatt tccctaataa ttgggttatt aaaatcactt ggtctcataa
    10201 aataatatat tttattagtt tataatttat ttaaaaatat ctattttttt ttatatttga
    10261 taatttcatt ttttttcttt taaatatcaa aaatacaaaa atttattcta ataatcaagt
    10321 ttttaaataa aaaaaaatac aaattaaaat atattaaacc aaatttttgt gtataaaaat
    10381 gatttcaaac ccatgttgtt tttacgaaac atacactaaa aacagaatac ctagccgcta
    10441 ggtaacaaat gaattgatga tatttttaaa aaacctacaa gagcggtata ggagaatcga
    10501 actcctgtct ctgccttggc aaggcagcat aatctgcata tgctccacta tacgaatacc
    10561 gcaagaatgg taacaagatt gtatttacgt gctctaccaa ctgagctaca gactcacaaa
    10621 ggaatcggga tggatttgaa ccatcgacca cgagcttaaa aagcttattt ttattgctgt
    10681 aacaatcttt taataccaat tagctcggaa gggtggattc gaaccacatt ttaaccagac
    10741 acggctttta aagtgtcact ttaaagctat agctgtcatc tgaggttacc tatacaggaa
    10801 ttcaaacctc tgccattccg aatgttgtct gtctctccag acttgtcaca cctattttgt
    10861 attctgttat ccttcttgga atcctaacgg taaaatggtg tcaaaaacca gtattgtgac
    10921 cccgatggga ttcgaaccca tgaccccatc attaaaagtg atgtgctcta ccaactgagc
    10981 tacggagtct tagacacaat gtgcgctctg atgtagggtc ctaacccctc aaacttctag
    11041 gcaacagggg tgcttacgac ccttttcccg tgcgcctacg gtagatgctt tcaaagctcc
    11101 catcagcatt gtgtttggta ctgcgtatgg gattcgaacc catgtgaatc ctttcggata
    11161 accagattga aaatctggcg acctagaccg ctagtcgaac gcagcatttt gcgaccttat
    11221 cctcattata tggtttcaag gtaggttact ttcagtttag tcgcttcgcc ttctaacttt
    11281 tcatggtatc cccgttatac atccagatat tattccacga atttcggtat tgatacctac
    11341 accatagtcc gagtaacgcg gacgggactc gaacccgcac gggcgattgt atgcccccag
    11401 agtgaaaatc tggtcactta ggccatttgt ataccgcgtc atataaaata tgtaataggg
    11461 taggtcgcat ttctgccatg tattattaca tatttttaat ttgtcatgct gcggagactc
    11521 gaactccgat tgtaagattg aaaatcttat ttcctgacct gttagaagac agcacgaaac
    11581 taccttacca tttaactccc tctaatactg tcatcggcct gataaggttg tgggctggtc
    11641 gatgaaggaa tcccatttaa ttctacgcat ccttccttcg cctttcggtt acttgcggtc
    11701 tatgaattaa taacagtcgt catgtggggg cagagggatt cgagcccact gcgtttctta
    11761 tgtgttggat tcgccacaat gctccaaggt tattacactt caattattta atttttctaa
    11821 ttttccattt ttaacataat aaaatttttt ccatgtatca ccaataaaat cctctaaatt
    11881 taccaattca cttatcttaa ttttatttgt atgaattaat ctatgacaat taggacaaac
    11941 ataagttaaa ttattatgtt catcagtacc accattttta cgttctaata tatggtgaat
    12001 atcgccaacc accccttcaa cataccatcc gcattttgaa catggcaatt tcattcgttt
    12061 taatatttta ctaatagttc ttttacttaa atcatataaa gtagttatat gcaaggctaa
    12121 gaccgtgcac ttaatcaaca tttgtgggtt catccgtaaa agaaaaaagc ccacgctctt
    12181 ttgctttttc aaggcaatta gggttaatat cacaaccaat aaagttccgt ttcaaatctt
    12241 tgcatacttc tgctgttgtg aaacttccag aataaaagtc tgcgactaaa tcaccttcat
    12301 tgctgcttgc ttttataaat c
//
LOCUS       DAOTJA010000014        11848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_5816_length_11848_cov_5.841855, whole genome shotgun sequence.
ACCESSION   DAOTJA010000014 DAOTJA010000000
VERSION     DAOTJA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5816_length_11848_cov_5.841855"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1397..1588)
                     /locus_tag="P5241_01600"
     CDS             complement(1397..1588)
                     /locus_tag="P5241_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32247.1"
                     /translation="MTSSASSSEICYLTSGTKKVNLTVTDAANKTASTSCQATLTTSK
                     CGSSGGGSTPPPVGYDENS"
     gene            complement(1598..1804)
                     /locus_tag="P5241_01605"
     CDS             complement(1598..1804)
                     /locus_tag="P5241_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32248.1"
                     /translation="MVANAQYTISEYGSEEAAKSACMKVCQAGGGTGAPLSVSCTGTI
                     TNTANCCIKWESTVSGGNLPFSYK"
     gene            complement(2255..3238)
                     /locus_tag="P5241_01610"
     CDS             complement(2255..3238)
                     /locus_tag="P5241_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRV32249.1"
                     /translation="MNLTEKNLGSIVAGYHYLTAYIGNDTTQETQIGQSTLLYSLDPQ
                     ANKTHQFTFTCPSPGNYYLKFITDSQYNVKEDNKNNNVLIANIQCTTGDSNYTGTYKF
                     TCDTVSKQCIVDSQGLFNTPEDCMASCSKSTGTNPGTETEKPNLQLNIDNPGNCQANK
                     FVMPFVLYNAGGGKAGSFKIAILKDTLTSTPVCTANIPSLDPGKTTKVNCDYVCQDTK
                     PHDLYFMVDYGNQISESNENDNIVKTVSPFACPAGCVGSANAGGGTGGDTGGDTGGGT
                     GGGTGGGTGGGTGFYLSWFTVQPSDVNTIVDNPISGGLKPYRKAPHTPVFS"
     gene            3207..3341
                     /locus_tag="P5241_01615"
     CDS             3207..3341
                     /locus_tag="P5241_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32250.1"
                     /translation="MLPRFFSVKFIVVSLPTVNVFGEKSDKVKSAPIVPPTLQTDAHS
                     "
     gene            3351..3509
                     /locus_tag="P5241_01620"
     CDS             3351..3509
                     /locus_tag="P5241_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32251.1"
                     /translation="MPVESKTHCQVEVLQEYFEVSATLPQLTLAVRVLFNLFDSVILL
                     TGSKIILK"
     gene            complement(3533..4348)
                     /locus_tag="P5241_01625"
     CDS             complement(3533..4348)
                     /locus_tag="P5241_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRV32252.1"
                     /translation="MKKSLYFILPIITGILVISFLFTIYNKPISADSCPFVNDLHIGD
                     ESNEVALLQLFLVRTGYYKEALITGYFGNLTSKAVKNFQKANGVYTAGFVGPDTRKAL
                     CSKYTTSCPFVSSSLKKGVTSSEVKMLQLMLISQTNFPSDMITGYFGPLTESHLKAFQ
                     SDNNLIANGIVDFNTQQKLCTLYTNTITLMSSNSGLTGGGNIDLAVSDLSYAPTQNIQ
                     VGSTIIFGIKEKNLGISDAPAHSYDLYINEYRLVSENLSALKSGQERAVNIQQ"
     gene            complement(4435..4818)
                     /locus_tag="P5241_01630"
     CDS             complement(4435..4818)
                     /locus_tag="P5241_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRV32253.1"
                     /translation="MDDSYNASPVAVSFSLELLNTLPANRRIFVFGDMKELGSYTEQA
                     HRQVGDEIIKHNIDLVINIGNYTENTYNQLINNGFKADNVYYFKNLLDAIDLLQNIIQ
                     TGDIILIKGSHSMGLDKIVQALAKN"
     gene            complement(4822..5412)
                     /locus_tag="P5241_01635"
     CDS             complement(4822..5412)
                     /locus_tag="P5241_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV32254.1"
                     /translation="MFAFKLLLFRQKDYPEILIIEMGADRPGDIEYLCNILKPKIGII
                     TNIGDIPVHIEYFQNIEDLYCEKSKLIKCLPPEGAAILNYDNKKILDFKNLTNASVIT
                     YGFDEGAEVRASNFKIIPQNNLDESEMYFRIEYGGTSVPFQIAGVFGKNYAYAFLCAI
                     SVGIYFNINLVESLEYLKDYKKLPHRTNILPAIKNS"
     gene            complement(5425..5697)
                     /locus_tag="P5241_01640"
     CDS             complement(5425..5697)
                     /locus_tag="P5241_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32255.1"
                     /translation="MNNKTNSKINKKRQLLTNILIRLTTKVLNKYKPIVIAITGNVGK
                     TSTKEAVFTILKSKYRCRRNEMNYNTPIGVAITVIGTKSGEGSLIA"
     gene            complement(5690..7450)
                     /locus_tag="P5241_01645"
     CDS             complement(5690..7450)
                     /locus_tag="P5241_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRV32256.1"
                     /translation="MSLRSGTKQFKTTRLFFISVIFCIIAIVAVSYLFVIQIKNHKNL
                     TAQAQSIQETKHIIQPKRGLIYFQDKNHNLISVAINKNYTTIYAVPKEIQQANKVEST
                     AKILSDILKIDYNSLLTKFLKINDEYEIVYAKIDDEKLINQIEQLKLKGIYVNNDELD
                     RYYPLGELGAHIIGFVGETKETKGEKIGQYGLEKFYNNVLEGQTGLFKGVRDAFGRLV
                     RSLTAEEEPVNDGSSLITSIDKNIQYKAEEELEKLLTTRLGSSGSIIIMEPKTGKILA
                     LANWPTYNLNEYSKVKDYKVYKNVAVEEALEIGSVMKVITMSAAIDSGAVQPDTKYID
                     KGSVTIDKKVIKNFHNEVYGECDMNKVLEQSINTGAIFAEQRTGNKIFLEYLKRFKLN
                     EKTGIDLPNEALNDIHQLELGPKDARDVYFATASFGHGIKISPISLVRAYATIANKGI
                     LVNPYIVEAIVNADGSKEDLSKQISEEQVIKPETVQKITQMMVRVVEHGYGKNAKIKG
                     YDIAGKTGTADIATQGSYSEDTIQSFIGFFPAYNPQFVILVKLDKPKIGTVASATVTY
                     TFRNMVQFLINYYNIPPDHE"
     gene            complement(7558..7746)
                     /locus_tag="P5241_01650"
     CDS             complement(7558..7746)
                     /locus_tag="P5241_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32257.1"
                     /translation="MSALIAFCLVLIVIVYISIDSSISRYDYEIYLLKQSIKQTTEEN
                     NTLKEKLIQNIAPEQIAV"
     gene            complement(7973..8833)
                     /gene="rsmH"
                     /locus_tag="P5241_01655"
     CDS             complement(7973..8833)
                     /gene="rsmH"
                     /locus_tag="P5241_01655"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRV32258.1"
                     /translation="MHTPVLLKEVLQYLNPQPNKNFIDGTVGEGGHSLAILNAIAPNG
                     KLLGIDLNQRNLDVAKEKFLQNNIPQERFILVQGNFKDIKQYIAQNNFYNIAGVLLDL
                     GLCSYFIDESKKGFSFKKNERLDMRFDTNSPLTAYDIVNTFKEQDIADIIYKYGEERF
                     SRKIAKSIVEARKVKNIETSQELADIIKHNKSSARVFQALRIYVNKELDNLQTAIPDI
                     LDILDKNGRLVIISFHSLEDRIVKNLFKQYSLSSRFRLLTKKPIIPSKEEIHKNVRSH
                     SAKMRAIEKI"
     gene            complement(9083..9256)
                     /locus_tag="P5241_01660"
     CDS             complement(9083..9256)
                     /locus_tag="P5241_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32259.1"
                     /translation="MPLTQSNSRAFSRLMLAGASEVKLDNQGRVLITDFLKKYANIEK
                     NIVIVGMYDRLEI"
     gene            complement(9284..9427)
                     /locus_tag="P5241_01665"
     CDS             complement(9284..9427)
                     /locus_tag="P5241_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32260.1"
                     /translation="MFLGEYKHIIDEKGRVFIPTKFHPQLKNGAVLTRGLDNCLFLFP
                     IKN"
     gene            complement(9926..9997)
                     /locus_tag="P5241_01670"
     tRNA            complement(9926..9997)
                     /locus_tag="P5241_01670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9963..9965),aa:Val,seq:cac)
     gene            complement(10515..10901)
                     /gene="rplL"
                     /locus_tag="P5241_01675"
     CDS             complement(10515..10901)
                     /gene="rplL"
                     /locus_tag="P5241_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRV32261.1"
                     /translation="MENLSQNVQDILSEIDSLSVLELTDLVKALEEKFGVSAAMPIAA
                     ATVASNNASTEEEEKSSYTVILQSPGEAKIQVIKVVRDVTGLGLKEAKDLVDAAPKPV
                     KENIPKTDADDLKKKLEEAGATVELK"
     gene            complement(10993..11490)
                     /gene="rplJ"
                     /locus_tag="P5241_01680"
     CDS             complement(10993..11490)
                     /gene="rplJ"
                     /locus_tag="P5241_01680"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRV32262.1"
                     /translation="MLNKIQKKDIIEKTKTLISNNNYIIFVTFSGVPTSNIETLRRDL
                     KALNCNLKMIKKTLGDIAIKEVGIDGVNIKKNYKGSIGFVFITGDLFSAINTLYSFKK
                     KNKDKFQILGAILEKQPSTVETINKLSSFSSLKSLYTSLVFVLNSNIQKLVFALDQIK
                     TKKSN"
BASE COUNT         4160 a         1870 c         1534 g         4284 t
ORIGIN      
        1 gatttttata tgtatcaaca attaaacttt tggacttgta ataataataa tagttataac
       61 tgccaatact aacaataata agtattatta taattataaa atactttcaa aaatgtttat
      121 gaaaatgttt tttgatcata atatataaaa ttttttattt gttatatact ttgattttac
      181 actattttgt tatataattc aataggttga ctaaaaatat aaaatatgct ataatactat
      241 agaccaaatg aatctgacga ttgctcctgc tatagttttg tcattaaaat aaaatagata
      301 tattaatact tcttcaaatt aaagcaatct ctattttcaa ataactattt taaatttatg
      361 gacaaaacta tagcaggaga ggaaagtccg aacacccatt tacataatta ttatgtatct
      421 aaaataatag tggctaacag ccatcctatc ttattagctc tttgagtaaa caagatagag
      481 aggtgcgaac agaaacggtt ttatttgaaa aaataaaacc accctataaa tatgttgata
      541 ttaaatcttt tgagtttatc aaaagataaa tataacactt attagggcga gctcaattgt
      601 aaaattgaga tgaaacggct aaatccttat tgggtgcaag agaaaaccct gtctcacttt
      661 tgatactaaa actttgtttt gttataaaaa atagaaataa caaactttta cactaatcct
      721 aattttaata tcaaaagtga gacagggaaa aagtatctcg cttgaccccg agagtaatcg
      781 aggggctaga taaataatcg tcacttattt tatatattta tatacataaa ataagcacag
      841 aattcggctt atagattcat ttggtcacaa tataaaaatc attatacaac aacaactccc
      901 tttttcaggg agttgttgtt gtataaatgt gtataaaata tctgtttgtt aataaaagta
      961 tcaataatta attacataca aacgctggac atgatactga tttggtttgt ccattgcaag
     1021 taacctcggc actataataa ttagttcctt ttaaattaga attacaaatt ttagcttgag
     1081 aagatccact tcctgacata atactattat tcttgccttg agaactatta gtccaatatc
     1141 atttataagt acatgcagat gaacttgaca aacctgatag attaataaca actgctgatg
     1201 aattggatgt tgatcctgtt accgaactag tactaccatt aatagtacaa taaggaaacc
     1261 aagaagaagt gtctggtggt gtataaggtt ttgcactgcc acaagcatta ttacaagttt
     1321 tggtagtata aatacaatta gtaggtgatt catatgcatt acaagcaaca caagtattgt
     1381 tcgaacattt atatcctcag gaattttcat catatccaac aggaggggga gtacttccac
     1441 cgccactact tccgcattta ctagtagtca aagtagcttg gcaactcgta gaagcagttt
     1501 tattagcagc atctgtaact gttaaattaa cttttttagt accactggtt aaatagcaaa
     1561 tctctgagct actagcacta cttgtcaaat cccctgttca tttatagcta aaaggaagat
     1621 tacctccact tactgttgat tcccatttta tacaacaatt tgctgtatta gtaattgttc
     1681 cagtacaaga tactgataag ggggcacctg tgccaccacc agcttgacat actttcatac
     1741 aagcactttt agcagcttct tctgatccat attcactaat tgtatattga gcattggcta
     1801 ccactctagt acaagtgcgt gttgaggtat tacaattata tccacattct ccggtatcat
     1861 cgacatttct ggtaatacca ctaccacaat cttgtttaca tttatcagga tctccataac
     1921 aaggttcagc ttttttacca tataaagaat agggagcatc attataaatg attatacact
     1981 tttcgctatt ttcagtagca ttagcaaaag cattagttgg tttagatgtt ttacaaacat
     2041 tattttgatc acaataagta atagtaggac aatatttacc agttgatgaa taagtatgac
     2101 aaatacgata atcggtgtta acttgttgag atccagaacc agttgattca taagcaccat
     2161 gggatccaaa tcctgttcaa gaacaatcat aacttgaacc atctccaaaa tctaaagtac
     2221 attttgctcc tttacctgaa taatcataaa tacttcaact aaatactgga gtatgtggtg
     2281 cttttctata aggtttaagc ccacctgaaa taggattatc aacaatagta ttaacatcag
     2341 aaggttgaac agtaaaccaa ctcaaataaa atcctgtgcc accacctgtt ccaccacctg
     2401 ttccaccacc tgttccacca cctgtatcac cacctgtatc accacctgtg ccgccgcctg
     2461 cattagctga tcctacacat ccagcaggac aagcaaatgg tgaaacagtc ttaactatat
     2521 tatcattttc attactttct gatatttggt taccataatc taccataaaa taaagatcat
     2581 ggggcttagt atcttgacaa acataatcac aatttacttt tgtagtttta ccaggatcca
     2641 atgaaggtat attagcagta caaacaggag tagaagttag agtatctttc aaaatagcaa
     2701 ttttaaaaga cccagcttta cctccaccag cattatagag aacaaatggc attacaaatt
     2761 tattagcctg acaattacca ggattatcaa tatttaattg taagttgggt ttctcggttt
     2821 ctgtgcctgg attggtacca gttgatttag agcacgaagc catacaatct tcaggagtat
     2881 taaataatcc ttgcgaatca acaatacatt gtttagaaac agtatcacaa gtaaatttgt
     2941 atgtaccagt ataattgcta tcgcccgtag tacattgtat gttagctatt aatacattat
     3001 tattcttatt gtcttcttta acattatatt gagaatcagt aataaatttt aaataataat
     3061 taccaggtga tgggcaagta aatgtaaatt gatgtgtttt gtttgcttga gggtctaatg
     3121 aatataacaa agtagattga ccaatttgtg tttcttgagt agtatcatta ccaatataag
     3181 ctgttaaata atgatatcca gctactatgc tgccaagatt tttttctgtt aaatttatag
     3241 tagtttcttt accaactgta aatgtttttg gagaaaaatc cgataaagtt aaatctgctc
     3301 caatagtccc tcctacctta caaaccgacg cacattcttg ataagaataa ttgcccgtag
     3361 agtctaaaac acattgtcaa gtagaagtat tacaagaata ttttgaagta tcagcaacat
     3421 taccacaatt aacactagct gtaagagtat tatttaattt gtttgattct gttatactat
     3481 taactggatc taaaataatt ttaaaataat aaattcctgg ctgggtacaa gttcattgtt
     3541 gaatattcac agctctttct tgcccagact tcaaagcact caaattttca ctaactaaac
     3601 gatattcatt aatatataaa tcataactat gtgcaggagc atcagatata cctaaatttt
     3661 tttctttaat accaaatata attgtgcttc ctacttgaat attttgagtg ggtgcatatg
     3721 acaaatcaga tacagccaaa tcaatatttc ctccgccagt taaaccacta ttagatgaca
     3781 taagagttat tgtattggta tataaagtac agagtttttg ttgagtatta aaatcaacaa
     3841 ttccattagc aatcaaatta ttgtctgact gaaaagcttt taaatgagat tctgttaggg
     3901 gaccaaaata tccagttatc atatctgagg gaaaattagt ttgtgatatt aacattagtt
     3961 gtaacatttt aacttcactg gaagtaactc ctttttttaa tgatgaacta acaaatgggc
     4021 aagaagtagt atatttacta cataaagctt tacgagtatc aggaccaaca aaaccagctg
     4081 tatatacacc attagctttc tgaaaattct ttacagcttt actagttaaa ttgccaaaat
     4141 atccagtaat taatgcctct ttataataac cagttctgac taaaaataat tgtaataaag
     4201 ccacttcgtt tgattcatca ccaatatgta aatcatttac aaatggacaa gaatcagcag
     4261 aaattggttt attatatata gtaaaaagaa aacttataac aagtatccca gttataatgg
     4321 gtaatataaa atatagagat tttttcataa taataatatt ttaaaatttt tattaaagtt
     4381 ttatattttt tataaagata tgaaacttta tataatataa ttatataaca ttttttaatt
     4441 ttttgcaagg gcctgaacaa ttttatccag acccatagaa tgtgatcctt ttataagaat
     4501 aatatcacct gtttgtatta tattttgtaa caaatcaata gcatctaata aatttttgaa
     4561 ataataaaca ttatcagcct taaatccatt atttattaat tggttataag tattttcggt
     4621 ataattacca atgttaataa ccaaatcaat attatgtttt ataatttcat ctcccacttg
     4681 acgatgagcc tgttcagtat agctaccaag ctctttcata tctccaaaaa caaaaatacg
     4741 acgattggct ggcaaagtat ttaataattc caaactaaac gaaactgcca ccggtgaagc
     4801 attataacta tcatcaataa ttcaagagtt ttttatagca ggtaaaatat ttgttctgtg
     4861 tggtagtttt ttataatctt taagatattc taaactttct actaaattaa tattaaaata
     4921 aatacctacg gatatggcac ataaaaaagc ataggcgtaa tttttaccaa acacacccgc
     4981 aatttgaaaa ggtacagagg ttcccccata ttctatccta aaatacattt ctgattcatc
     5041 taaattattt tggggaataa ttttaaaatt ggaggctcta acttctgctc cctcatcaaa
     5101 cccatatgta atgactgatg catttgttaa atttttgaaa tctaaaattt ttttattatc
     5161 ataattcaaa atagctgcac cctcgggagg caaacattta attaattttg atttttcgca
     5221 atataaatct tcaatatttt gaaaatattc tatatgaaca ggtatatctc ctatattagt
     5281 aataattcca attttgggct ttaaaatatt acataaatat tcaatatcac ctggccgatc
     5341 agcacccatt tcaataatta aaatctctgg ataatctttt tgtctaaata ataataattt
     5401 aaaagcaaac aaaatattta aacatcaagc aattagtgat ccttcaccac tttttgttcc
     5461 aatcacagta attgcgacac ctatcggtgt gttataattc atttcatttc tacgacatct
     5521 atatttagat ttcaggattg taaaaacagc ttctttagtt gaagttttac ctacatttcc
     5581 agtaatagca ataacaatgg gcttatattt atttaagact tttgtagtta atctaattaa
     5641 aatatttgtt aatagttggc gttttttgtt tatttttgaa tttgttttat tattcatgat
     5701 caggtggaat attataataa tttatcaaaa attgtaccat attgcgaaaa gtataagtaa
     5761 cagtagcact agcaacagta ccaattttag gtttgtctaa ttttaccaaa attacaaact
     5821 gaggattata agcaggaaaa aacccaataa aagattgtat agtatcctcg gaataagaac
     5881 cttgagtcgc aatatccgca gtacctgttt taccagcaat atcatagcct ttaattttgg
     5941 catttttccc ataaccatgt tcaactactc ttaccatcat ttgagttatt ttttgaacag
     6001 tctcaggttt tataacttgc tcttcagata tttgttttga tagatcttct ttagatccat
     6061 cagcattaac aatagcctct acaatatatg gattgaccaa tatgccttta ttggcaatag
     6121 tagcataagc ccgaactaaa ctaattggac taatttttat accatgacca aaactggcag
     6181 tagcaaaata aacatctctt gcatctttag gaccaagttc taactgatga atgtcgttta
     6241 aagcttcatt aggcaaatca atacctgttt tctcgtttaa tttaaaacgt tttaaatatt
     6301 ctaaaaatat tttattgcct gttcgctgtt ctgcaaatat tgctccagtg tttatagatt
     6361 gttctaatac tttattcata tcacattcgc catatacttc attatgaaaa tttttaatca
     6421 cttttttatc aattgtaaca gatcccttat caatatattt tgtatcaggt tgtacggcgc
     6481 cagaatctat agcagcggac atagtaataa ctttcattac cgaaccaatt tcgagagctt
     6541 cttcaacagc tacatttttg tatactttgt aatcttttac ttttgaatat tcgtttagat
     6601 tataagttgg ccaattagct aaagccaaaa ttttacccgt ttttggttcc ataataatta
     6661 ttgatccaga gctaccaaga cgagtagtta ataatttttc cagttcctct tcagctttat
     6721 attgaatatt tttatcaata gaagtaataa gtgaggatcc atcatttact ggttcttctt
     6781 ctgctgtcaa agatcgaacg agcctaccaa aagcatcacg aacaccttta aataatcctg
     6841 tctgaccttc taatacatta ttataaaatt tttctagccc atactgccct attttttctc
     6901 cctttgtttc cttagtttct ccaacaaatc caattatatg agcccctaat tctcctaagg
     6961 gatagtatct atctaattca tcattattaa catatattcc ttttaacttt aactgttcaa
     7021 tttgattaat tagtttctca tcgtcaattt tcgcatacac aatttcatat tcatcgttta
     7081 tttttaaaaa ttttgtaagc aatgaattat aatcaatctt taaaatatca ctcaaaattt
     7141 tggctgtact ttctacttta tttgcttgct gaatttcttt tggtacggca taaatagttg
     7201 tataattttt gttaatagca acactaatta aattgtgatt tttatcttga aaataaatta
     7261 acccacgttt gggttgtata atatgttttg tttcttgtat tgattgagct tgggcagtta
     7321 agtttttatg attttttatt tgaataacaa acaaataact caccgcaaca atagcaataa
     7381 tacaaaaaat aacacttata aaaaataagc gcgtagtctt aaattgtttt gtgccagatc
     7441 gtaaagacat aattattgat tagatgtaga tgtctgaata atattggcaa ctatagctcc
     7501 ctcagaaata ttaaaataat ctaatttaga aacttcaaca aaattattgc ttttagctca
     7561 aactgcaatt tgttctggag caatattttg tatcagtttt tcttttaggg tattattttc
     7621 ttctgttgtt tgtttaatac tttgttttaa aagataaatt tcataatcat aacgactaat
     7681 acttgaatct attgatatat agacaataac aattaaaact aaacaaaaag ctatcaaggc
     7741 agacaattta atgttcgcaa aaccacttaa ctgcgatctt atattacgtt taattttaga
     7801 tcttggaact actcacctat tagatagaac tgattgttgt ttgtagtatg aatttattat
     7861 tgtcatagta aaatgtttat ttttgtttta tactttttct tagatattag tataaaacat
     7921 tttttattaa gatcaatcgc aataatattt tttaatgtca aataataaat aactatattt
     7981 tttctatagc gcgcatcttg gcactatggg atctaacatt tttatggatt tcttctttgc
     8041 tgggaataat tggtttttta gtgagtagcc taaatcgtga tgataaacta tattgcttaa
     8101 ataaattttt aactattcta tcttctaaag aatgaaaaga aataattact aatctgccgt
     8161 ttttgtctaa gatgtcaaga atatctggta tagcagtttg taaattatcc agttctttat
     8221 ttacataaat acgtaaagct tgaaaaactc tggcactact tttattatgt ttaataatat
     8281 cagctaattc ttgagaagtt tcaatatttt ttacttttct tgcttcaact atactttttg
     8341 ctatttttct tgaaaatctt tcttcgccat acttatagat aatatcagca atatcctgtt
     8401 ctttaaaagt atttactata tcataagcag tcagtggaga atttgtatca aaacgcatat
     8461 ctaatctttc attttttttg aagctaaaac ccttttttga ttcatcaata aaatagctac
     8521 atagacctag atccaaaaga accccagcaa tattgtaaaa attattttgt gcaatatatt
     8581 gtttaatatc tttaaaattt ccttgcacca atataaatct ttcttgtggt atattgtttt
     8641 gtaaaaattt ttctttggca acatctaaat ttctttgatt taaatcaata cccaaaagtt
     8701 ttccattggg tgcaatagca tttaatatag ccaaagaatg tccaccttca cccacggtgc
     8761 catctataaa atttttatta ggttgtggat ttaaatattg taatacctct tttaaaagaa
     8821 caggagtgtg cataatataa caataacaat ttttaatttt tggaattctc taatgtaaaa
     8881 ataattttta attgtaaatt tttaaataat ggaaattaga aattggaaag aataaatatt
     8941 gatttctttt atatatgtaa tctgtgtttt atgaaataaa atttattaac aattattata
     9001 ttcctaagtt tgttaagttt tcggctatat cattagaatt tttttcagtt tgttgcttat
     9061 atgtttctca ggctgattct tctcaaattt ctaatctatc atacatacca acaataacaa
     9121 tatttttttc tatattagca tattttttta agaaatctgt tatcaatacc ctgccttgat
     9181 tatctaattt tacttctgaa gctccagcta acatcaatct tgaaaaagcc cgagaattgg
     9241 attgggttaa cggcaaagat tttatttttg ctgccagaac ttctcaattt tttattggaa
     9301 aaagaaacaa acagttgtct aaccctcttg tcaaaactgc accatttttt aattgaggat
     9361 gaaatttagt aggaataaaa actcttcctt tttcatctat aatgtgtttg tattctccta
     9421 aaaacataat ataaatttat attatatata gatggtttaa gcgattttag agaaactttc
     9481 taaaaagtaa gataaaaaat gaaaattatt attaggaaat tacaatggaa acaattatta
     9541 tttgggttta aaagatttgc acatattttg cgagttttcc catggttttc cactttctac
     9601 cacttttatt taattatact ccataaaatg ccaaatgtca ataccttgac taattttttt
     9661 tccacaggtt atccccaata caaatttatc aaaataaata agggagtata gggtaaattt
     9721 taattattta taatttatta caaaatataa atttaaaaac atttattatt tataatattg
     9781 atataaaaac ttgctatata ttaaaaaaga acactgaatt gcagtgtcca tttttttata
     9841 tgtattataa ctttatttta aacttgtata ttttataaaa cagtaaatgt tataggaatt
     9901 aaatttataa ctttcctatt ttttgtgcgc atagttggtt tcgaaccaac gaccttccgc
     9961 atgtgaaacg gacgctctac cactgagcta tacgcgcgca taagataaaa aacataaggg
    10021 catagaataa aacaatgagt attaaataat caataattaa ctaaaagagc aataagacaa
    10081 tcaaacaatt aagacaaaat aaaacatacg gacatagaat aaaacaataa gcatcggata
    10141 atcaataatt aactaaggac gcaaggatac aatcaaacaa ttaagacaaa ataaaacata
    10201 taagataaaa aaacatacgg gcatagaata aaacaatgag tattagacgc taaacaatta
    10261 actaagagaa cgagaataca atcaaacaat tagaaattaa tgttaacagg taataattat
    10321 gataatagca tatttttgaa aatttgtcca gcaagtttca ttttatttag cttggttgtt
    10381 tatattaaaa tattaaaaca atatatgata caacaaaaaa tctccgtaca taataatata
    10441 ctccgtatat agcaatatac ggagtatatt ttgtacatat ttttaataac tttcaaaata
    10501 aaaaatgcaa acaattactt cagttctaca gtggcaccag cttcttctaa tttctttttt
    10561 agatcatccg catctgtttt aggaatattt tctttgacag gttttggagc agcatccacc
    10621 aaatcttttg cttcctttaa tcccaatcct gtaacatcgc gcacaacttt aataacctga
    10681 attttagctt cacctggaga ttgtaatatt acagtataag aagatttttc ttcttcttca
    10741 gttgaggcat tattactagc aacagtagca gcagctattg gcatagcggc tgaaactcca
    10801 aatttctctt ccaatgcctt aactaaatct gttaattcta aaactgatag agaatcaatt
    10861 tcagatagaa tatcttgtac attttgcgat aaattttcca tgatatataa acttagttta
    10921 tagctatcag cctaaacccc aagatcaaca attattctat tactctatag tttacggcta
    10981 attgctaaat tattaattac tcttttttgt ttttatttgg tcaagagcaa aaaccaattt
    11041 ttggatatta gaatttaaaa caaaaactaa tgaagtatat aaagatttta acgaagaaaa
    11101 actcgataat ttattaattg tttcaactgt actgggttgc ttttccaaaa ttgcaccaag
    11161 aatctggaac ttatctttat ttttcttttt aaaactatac aaagtattta tagctgaaaa
    11221 caaatcccca gtaataaaaa caaaaccaat tgaaccttta taatttttct ttatattaac
    11281 accatctatg cctacttctt taatagcaat atcgcccaaa gttttcttaa tcatttttaa
    11341 attacaattc agagccttta aatcacgtct tagtgtctca atattagatg ttggaacccc
    11401 tgaaaaagta acaaaaatga tatagttgtt attggaaatt aatgtttttg ttttttctat
    11461 aatatctttt ttttgaattt tgtttaacat attttatatt taaaaaagaa aaaatccgtc
    11521 tcccaacaga tttgttcgac cctaaataag acttatttgt gtgcttattt tctttgggag
    11581 ttatgcaaat atactaccat attttttaaa aaaatacaac tagttgcaca tcataatacc
    11641 ttaaatattt agtgctctat aaagtatcag atgtgtttgg acttaatcgg tacctatata
    11701 tattagcaac gtattagcat atttagcaat ctaatattta aaaaccaagt ttttgaaata
    11761 tatctgatat atcggaatat aaaagtaaga agcatagact aatacccctt atactcttca
    11821 atagactgac ctcaaccagg actggtaa
//
LOCUS       DAOTJA010000015        11562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_5966_length_11562_cov_5.276180, whole genome shotgun sequence.
ACCESSION   DAOTJA010000015 DAOTJA010000000
VERSION     DAOTJA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_11562_cov_5.276180"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..805)
                     /locus_tag="P5241_01685"
     CDS             complement(377..805)
                     /locus_tag="P5241_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32263.1"
                     /translation="MQYLDTIESHFTNFVEKLLRRSRIIVLRIDNNISKTLNDIKKKK
                     IIEQEKLKTKHISFSNLNNVRSSSKTLALNKLKKEELALLEAFINDRNNLESYKNLAR
                     FYLNHNEYKNCRNVLLEALAIDPNDKIISDLFVVLYEKTK"
     gene            complement(821..958)
                     /locus_tag="P5241_01690"
     CDS             complement(821..958)
                     /locus_tag="P5241_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32264.1"
                     /translation="MFDFIFLFLIVIGVAGIIYIYKKYQQDIKKEFEQKDNILLNNKN
                     K"
     gene            complement(978..1829)
                     /gene="tsf"
                     /locus_tag="P5241_01695"
     CDS             complement(978..1829)
                     /gene="tsf"
                     /locus_tag="P5241_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRV32265.1"
                     /translation="MSIDLIKQLREETGVSVMECKKALEKANNDIEQAKIILKEEGLV
                     NASKKSSRETKNGVIVSCVSPDGKNGAILELRCETDFVAKNEEFIQLANNLVKEILNQ
                     RPTDTTDLLAKTKESETISDVIKGTIAKFGENIEIGQFNLLDTINGIIVSYIHNGNKV
                     GVLLQLDCANGIDINNDSIKQLGKDIAMQIAAMNPQFVKAEDIPSDKLEFYKKEFEQD
                     IDMSKPEDIRNKILEGKFAKKYEDICLLNQHFIKDDTKTIGDLLKAFNIQNNINITIA
                     KFIRYQI"
     gene            complement(1942..2583)
                     /gene="rpsB"
                     /locus_tag="P5241_01700"
     CDS             complement(1942..2583)
                     /gene="rpsB"
                     /locus_tag="P5241_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRV32266.1"
                     /translation="MEIDQKKIQEMIQNGLQYGHRRSKTHPKAAPFILSTKNNIEIIN
                     LETTFFKLQEAIAVIKNTLDQKGLILFVAAFPTSKDFIQDIARSLNQPYVLNRWTGGL
                     LTNFKAINERIKYYSDLKQQIETGELSKYTKKEQLDKEKELEKLKIKFEGLTSLTRKP
                     DLIFIVDPGYNKTAVLEAKKSKIPIIAILDNDDDPTLIDYPIPANDSSLSSIS"
     gene            complement(2652..3143)
                     /locus_tag="P5241_01705"
     CDS             complement(2652..3143)
                     /locus_tag="P5241_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32267.1"
                     /translation="MGDRLIVVRTSMFDDIKNGVDNLIVEKKTGKTICAIDDVSSLST
                     TFVNKTEKIFHQNNAINVKNIKYGLTIKDGQTLLTHLERVPVFCIGIPLSGVDQFANY
                     ISDSIDSKPSLEEQKLMEYYYRLLEMQIRQYDLSIVAADLEKLIPDTIKKELKYYKTQ
                     NTR"
     gene            complement(3153..3482)
                     /locus_tag="P5241_01710"
     CDS             complement(3153..3482)
                     /locus_tag="P5241_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32268.1"
                     /translation="MVDKSQKFNRPNYYNNNDNKNISNKTSKSFEQVVKQETIEKLNN
                     LIQKLALKLKKENINVTNDCRIDMDSFESIYSDIKNDKRYIETIRGKMSNTDYIGEPV
                     EILTTIV"
     gene            3990..4268
                     /gene="rpmE"
                     /locus_tag="P5241_01715"
     CDS             3990..4268
                     /gene="rpmE"
                     /locus_tag="P5241_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRV32269.1"
                     /translation="MKKNIHPKYNDQCKVKCVCGSTFTVGSTKDNLDIEVCSECHPFF
                     SGKEKLIDTEGRVKKFQSRVTKSTSLKDKVTKKKIKKATRNNSKNKTK"
     gene            4605..5045
                     /locus_tag="P5241_01720"
                     /pseudo
     CDS             4605..5045
                     /locus_tag="P5241_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            5079..5282
                     /locus_tag="P5241_01725"
     CDS             5079..5282
                     /locus_tag="P5241_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRV32270.1"
                     /translation="MDEVTINPEDVEIEFYRSTGHGGQNVQKVETAVRLKHKPTGIIV
                     QCQTERFQRQNRDRAMAVLQAKI"
     gene            complement(6151..7329)
                     /locus_tag="P5241_01730"
     CDS             complement(6151..7329)
                     /locus_tag="P5241_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRV32271.1"
                     /translation="MVFNEFYMNKNKTLTPLKQKGVDTGAGLERMVLVTEFPKFQTTH
                     NTIYNTDIFMPMLDYINTNSQKYDEKLFRIIADHFKASIFLIADGLHPSNVDRGYVLR
                     RLIRRIINYQQRIVLKDNFEKDLLIIVQNIYNNFYPEINNTDNILSVYNTERDKFLST
                     LEKGLKELNKILDKQPKILDGKTAFYLYESFGFPLEMIIEEASAKNVIVNTSEFAQEF
                     EKHKNISRAGAQHKFGGHGLEHNITLTESNIRKTKLHTATHLLLQALREKFGNSIYQK
                     GSDINEGRLRLDFPFERKLSEQELKDLENIVNDKIKQALPIESKTLSLEQAKKENYLG
                     VFTAKYPPQVTVYNIGNWSKEICAGPHVNNTKELGHFTILKEESSSAGIRRIKATLTN
                     "
     gene            complement(7462..7917)
                     /locus_tag="P5241_01735"
     CDS             complement(7462..7917)
                     /locus_tag="P5241_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRV32272.1"
                     /translation="MKSFEVRDTFLKFFKNYNHIHIPGVSLLPEGDSNALFTVAGMQQ
                     FKDYFSGIKNPFLDIHPNIMKPLGSNNIVTIQKCLRTIDIDLVGDEKHLTMFEMLGNF
                     SFAGYFKQEMIPMAFEFVTQVLNIPVEKLSISVFAGDSEISFDQESYDI"
     gene            complement(7952..8599)
                     /locus_tag="P5241_01740"
     CDS             complement(7952..8599)
                     /locus_tag="P5241_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRV32273.1"
                     /translation="MYVSSLNAQKNIQSPIGFLEDLFHFRTIRNEYYQLKTNQILNAN
                     NLYLQSVCVKPQLYNTIQANALISDIEGASGIFIINKGRADNVKVGMNITLDNSVLVG
                     RVVEVFDNYAKVESLYSPNINISVINSISNEVALVKRDNKGFLKLTLFSDASKFKIGD
                     PLVTSLDNKEFLRGLLVGQISNIVSQQSLNKPDIFIDPFFKLPYLNTVYIITTYP"
     gene            complement(8749..9807)
                     /locus_tag="P5241_01745"
     CDS             complement(8749..9807)
                     /locus_tag="P5241_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRV32274.1"
                     /translation="MNMQFLQNIFKKSDSFFQNIGLDLGTTKISVYVKGKGIVLKEPA
                     VVALNTKTDQIIAIGKEAEAMLGKTPSHISAIKPIVNGVISDFEVTEKLLRFLFQKIK
                     EKNIFLGPKVIVGVNSLGTEVERKAVEDAVRNAGAKEVYLIEKALATAIGAGLLLNEA
                     RGNLIVDIGGGITEVSIVSLGGIVVTKNIRIAGNKLNDDIIYFVRNKFKLAIGEQTAE
                     KIKITIGSALERNTDETMTIRGRDLAKGLPKEVKVTESEIREAMSESLQTIVQIIKDT
                     LEIAPPELIADIIEKGILLTGGTSLLKDMDTLIEKATGLKVNISDDPVNSVVKGEGII
                     LENFEAMKPYLLTLKTTL"
     gene            complement(9850..10233)
                     /locus_tag="P5241_01750"
     CDS             complement(9850..10233)
                     /locus_tag="P5241_01750"
                     /inference="COORDINATES: protein motif:HMM:NF020742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32275.1"
                     /translation="MRLEVGQRELDNNVVSAVRRDNFEKSNLDIKDILNTIPELLLNI
                     QNDLLQKSRKLKNDLTIDVSDYNEFKKAINDGKFVRAFWCEDEQCEEAIKQETKASNR
                     CLELDRIESNESGECIYCHKKAKRR"
     gene            complement(10252..10683)
                     /locus_tag="P5241_01755"
     CDS             complement(10252..10683)
                     /locus_tag="P5241_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32276.1"
                     /translation="MGLKSQQEKFAGAKNTYTIEMVAPNGKSIQMCTSHDLGDNFAKV
                     FNIQYIDENGEKQYVYQNSFGLSTRSLGCLILAHGDDFGLILPPKVAPIQIVILPIIT
                     DNDNKEIIQFSENIYKQLSSIGIKAKLDVDFKHTLGYRINE"
     gene            complement(10900..11109)
                     /locus_tag="P5241_01760"
     CDS             complement(10900..11109)
                     /locus_tag="P5241_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32277.1"
                     /translation="MDKQFKEDGIMNVYFPAFIPMSLFEKEKEHIKGFAPELFMIKSI
                     GDEDLLDPYVLRPTSEAIIDKALSK"
     gene            complement(11287..>11562)
                     /locus_tag="P5241_01765"
     CDS             complement(11287..>11562)
                     /locus_tag="P5241_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV32278.1"
                     /translation="PVGIAVLTQKISKMGSGFFFAFMASLSINLAFMNLLPIPALDGG
                     KLLFLLFEQIKGSALNKKFENYATIGGFAFLMILVLIVTIKDVISLF"
BASE COUNT         4158 a         1743 c         1481 g         4180 t
ORIGIN      
        1 cgctgagcta agacggcaac taactaactt ctaaaaaata ttatatatta tgtaaggtgc
       61 ttggtttctg tttcgagcaa cacttcgtga acgctcttcc ggggtaaact acgtatttct
      121 attgtgtttg ataaatcaaa cacgtatttt gaagtttacc cgctgaaact caatactttg
      181 agtttcagcc cgctgagcta agacggcaac taactaactt cttaaaaata ttatatatta
      241 tgtaaagcgc ttgacaatat taaaataact aaatctatcc ttttctatac caatactttt
      301 aaaataaata tcaaaatacc acaattagat attactaatt aatacttttt tatttgttaa
      361 tgtgcataca gaatcactat ttggtttttt catacagaac aacaaacaaa tcagaaataa
      421 ttttatcatt aggatcaata gcgagagctt ccaataaaac attacggcaa tttttatatt
      481 cattatgatt taaataaaat ctggctaaat tcttatatga ttctaaatta tttctgtcat
      541 tgataaacgc ttctaacaga gctaattctt cttttttcag tttattaagt gctaaagttt
      601 ttgacgaaga acgtacatta tttaaattag aaaaagaaat atgttttgtt tttaatttct
      661 cttgttcgat aattttcttt tttttaatat catttaaagt ttttgagata ttattatcta
      721 tacgtaaaac aataatccta gaacgacgca acaatttttc tacaaaatta gtgaaatgtg
      781 attctattgt atctaaatac tgcatattaa taaccttgga tcacttattt ttattattca
      841 gcagtatatt gtctttttgt tcaaattcct ttttaatatc ttgctgatat tttttataaa
      901 tataaattat accagcaacc ccgataacta ttaaaaaaag aaaaataaaa tcaaacatag
      961 taaatttata ttatacatta aatttgataa cgaataaatt tagcaatggt tatattgata
     1021 ttattctgta tattaaaagc cttaagtaaa tcaccaattg tttttgtatc atctttaata
     1081 aagtgctggt ttaatagaca aatatcttca tattttttgg caaatttacc ttctaatatc
     1141 ttattacgaa tatcttctgg tttagacata tctatatcct gctcgaattc ttttttatag
     1201 aattctaatt tgtcagatgg tatatcctct gccttaacaa actgagggtt catagcagct
     1261 atttgcatag caatatcttt gcctaattgt tttatagaat cattatttat atctatacca
     1321 ttagcgcaat ctaactgtaa caaaacacct actttattac cattgtgaat atacgaaaca
     1381 ataataccat taatagtatc taataaatta aattgtccta tttcaatatt ttctccaaat
     1441 ttggcaatag tgcctttgat aacatcacta attgtttctg actcttttgt tttggctaat
     1501 agatctgtgg tatctgttgg tcgttgatta agtatttctt ttactaaatt attagcaagt
     1561 tgtatgaact cttcattttt agcaacaaaa tcagtttcac aacgtaattc taaaatagca
     1621 ccatttttac catcaggact tacacagcta acaattacac cgttttttgt ttctcgagaa
     1681 gattttttgc tcgcatttac caaaccctct tctttaagaa taatttttgc ttgttctata
     1741 tcattgttag ctttttctaa agcttttttg cactccatta ccgagactcc tgtctcttcg
     1801 cgtaattgtt taattaaatc tattgacata atatattatt tatttattat taaagtatgc
     1861 aattaaaaag taataaaatt atttttaaac ttggcttata gttggatttg ttaatatttg
     1921 tgatttaatt tcattaataa ttcaagaaat agaagataat gagctatcat tggctggaat
     1981 aggatagtca atcaaagtag gatcgtcgtc attatctaaa atagcaatta taggaatttt
     2041 acttttttta gcttctaata ctgccgtttt attgtatccg ggatctacaa taaaaatcaa
     2101 atctggcttg cgagttaaag atgttaaacc ttcaaactta attttgagct tctctaattc
     2161 cttctcttta tctaattgtt cttttttagt atattttgat aattctcctg tttctatttg
     2221 ttgttttaaa tcagaataat atttaatccg ttcattaatt gctttgaaat tagttagaag
     2281 accacctgtc cagcgattta aaacatatgg ttgattcaaa gaacgagcaa tatcttgaat
     2341 aaaatcttta gatgtaggaa aggcggcaac aaacaaaatt aacccctttt gatctaatgt
     2401 attttttata actgctatgg cttcttgtaa cttaaaaaaa gtagtctcca aattgattat
     2461 ttcaatattg tttttagtag acaaaataaa aggagcagct ttgggatgcg ttttagatcg
     2521 acgatggcca tattgtaaac cattttgaat catttcttgt atttttttct gatcgatttc
     2581 cattgataaa ttttatttat gtttttgttt aatttatttc attttatcat ataattaaaa
     2641 ataaatcaag attagcgcgt attttgtgtc ttataatatt ttaattcttt tttaatagta
     2701 tctggtatta atttttctaa atctgctgcc actatactca aatcatattg cctaatctgc
     2761 atttctaaca atctgtaata gtactccata agtttttgct cttccaaaga tggtttacta
     2821 tcaatagaat cagatatata attcgcaaat tgatcaaccc ctgaaagagg tattccaata
     2881 caaaatactg gtactctttc taaatgtgtc aagagggttt gaccatcttt aatagtcaaa
     2941 ccatatttta tatttttaac attaatagca ttattttgat gaaatatttt ttctgtttta
     3001 tttacaaatg ttgtggataa tgatgacaca tcatctatag cacaaatagt tttaccagtt
     3061 ttcttttcta caattaaatt gtctactcca ttcttaatat cgtcaaacat tgatgtcctt
     3121 actactatta atctatcacc caatacttta tttcaaacaa tagttgtgag aatctctact
     3181 ggttcgccta tataatctgt attggacatt ttgcctctta tagtttcaat atatctttta
     3241 tcgtttttta tatctgaata aatactttca aaactatcca tatctatacg acaatcatta
     3301 gtaacattaa tattttcctt tttgagtttt aaggctaact tttgtatcaa attatttaat
     3361 ttttctatag tttcttgttt aactacttgc tcaaaagatt ttgatgtttt gttacttatg
     3421 tttttgttgt cgttattatt ataataattt ggacgattaa atttttgcga tttgtctacc
     3481 ataataaata gtttaggtct aagaataata taaaaactaa accaaaatat actaattaaa
     3541 caggtatttt gatgataatg gacttttatt tataataaag gtattgcagt agtatgctat
     3601 cataattttg ataaaatcgc aatgggcttg atggttaagc tcaaacacat ttgatactct
     3661 atagaatact aaaaattacg gtattatgga attttgcaat tcacaaaaaa caattcacag
     3721 aaatttatat atactgggat taaagaaatt caatcagtgt attataatta tataattcag
     3781 caagatcaga ttatgcttga taataaaaaa ccacattttt cttttataat taatatctat
     3841 aatcaaaagc cttaaatgtt ataaacacat acatgggctt gatttttatg taaaaatagt
     3901 gtaatataat aattatatac aaagataaca tattagccta taaattattg aataataata
     3961 aagaataata ataaacaaaa tttttaaata tgaaaaaaaa tatacatcca aaatacaatg
     4021 accagtgcaa agtaaaatgt gtctgtggta gcacttttac agttggttct accaaagaca
     4081 atttagatat tgaagtatgc tcagaatgcc atccgttttt ctcgggaaaa gaaaaattaa
     4141 tagatacaga gggtagggtc aaaaaattcc aaagtagagt aactaagagc acttcgttaa
     4201 aagacaaagt tactaagaaa aaaatcaaaa aagctacaag gaataattca aaaaataaaa
     4261 caaaatagtt atatatttca attgtaatat aactattttg ggtaattttg tataaaataa
     4321 taaaaaaaat aaaaaaatag cttgctataa aagtcaacaa attacagaaa gtaaatttta
     4381 aatttactat aaaaacaata aaaaaacgat tgctaataat ttaaaacatt agtatggaac
     4441 aaatatgaca aacaaaatac gaacaattat cacaagagcg tgaaaatatt atccaaaagc
     4501 tgtctgatcc tgctattatc aaaaatcaag aagaatatat agctattaac caaaaactaa
     4561 aagagataga taaatattat tctttattaa aagagtgaga aaaaattgac aagcatttag
     4621 aagaagatca gtatattata gaaacagaaa ttgatgacga aattattcaa gaagcacaaa
     4681 cagatgtaaa caaaaatatc aaacgattaa acgaaatcaa aaaggcattg aataatttag
     4741 aacataccaa aaaccaatca gaaaatgata taaatgatat aattttagaa attcgtgcag
     4801 gtacaggagg agaagaagcg gcattattcg ctggtgattt gttaagaatg tatacacgat
     4861 ttgctgaaaa acaaaattga aaagtaagta taatagacgc caattctaca aaccaagaag
     4921 gctataaaga agttatagca gaaatagaag gaaaagatgt atataaactt ttaaaaaatg
     4981 aagcaggcgt acacagagtt caaagagttc ctataacaga aaaaaataat agaattcata
     5041 cttcaacagc aacagtagca gtattaccaa aaccaaaaat ggacgaagtt actataaacc
     5101 ctgaggatgt agagatagaa ttctacagat caacaggaca tggcggacaa aatgttcaaa
     5161 aagtagaaac tgctgtaaga ttaaaacata aaccaacggg tataattgtt caatgtcaaa
     5221 ctgaacgatt ccaacgacaa aatcgagata gagctatggc agtattacaa gctaaaattt
     5281 gagaatatga aaaagaaaaa gttattggta accaagatac gtcaagaaaa caacaaatcg
     5341 gtcaagcaaa aagatgcgaa aaaatcaaaa cctataattt tccacaagat agactaacag
     5401 accaccgtat tggtcaaagc tgacacaatt tagaaaaaat tatgcaagga gatattgaag
     5461 agatattaaa agaaacaatg aatgacagta aattctataa agacacagat aacgacgatt
     5521 tttaataaca ataatacaaa ccaacacaac aaatcctaaa acaaatcccc tcttattccc
     5581 aagtaaaaat atagacttaa aaaatataca agaaacagta gcctaaaaaa gatactcgaa
     5641 acaaaagcgt aaaaaatata taatttagta ataaacgaaa agttaataca attgttacca
     5701 aaatatacat taccaaaata tatattacca aaatataaat acaatataaa tcagaaatat
     5761 acaacaataa ataaaatata aataaaaata gttactgaca tttacatatt cttgggaata
     5821 tgtaaatgtt ttttattact ggcaatgttt taattgctaa caatgttgtt tatgactgaa
     5881 atagtggttt attattgata atgctattta tgactttttt attaccgaat agtcaaaaac
     5941 atttacataa acatttacat attcttggga atatgtaaat gttttttcga aatatagaaa
     6001 attaaacttg ttcgtttaaa actataagct gtttagtatc cacgagttta gtatccacga
     6061 gtttagtatc cacgagttta gtatttgcta gttgggctaa ttggaaacta ctttactacg
     6121 cgagatttcg taaagtaaga ctaattgtgt ttagtttgtc aaagtggctt tgatacggcg
     6181 gatgccagct gatgatgact cttcttttag tattgtaaag tgccccaatt cttttgtatt
     6241 atttacatga ggaccagcac aaatctcttt ggaccaattt ccaatattat aaaccgtgac
     6301 ttggggagga tattttgccg tgaacacacc taaataattt tctttttttg cttgctctaa
     6361 tgataaggtt ttagattcta taggcagagc ttgcttaatt ttatcattaa caatattttc
     6421 taaatctttt aattcttgtt cgcttaattt tctttcaaat ggaaaatcta atcgcaaacg
     6481 cccctcgttt atatctgatc ctttttgata aattgaattg ccgaacttct cgcgcaaagc
     6541 ttgcagtaat aaatgagttg ctgtatgtaa tttagtttta cgtatattgg attctgttag
     6601 agttatatta tgttctaaac catgaccacc aaatttgtgt tgtgctccag cccgagatat
     6661 atttttgtgt ttttcaaatt cttgcgcgaa ttcactagta tttactataa catttttagc
     6721 tgatgcttcc tcaataatca tttccaaagg aaaaccaaat gattcatata aataaaaagc
     6781 agttttgcca tcgagtattt ttggttgttt atctaatatt ttattcagtt cttttagtcc
     6841 tttttctaga gtgcttaaaa acttatctct ttctgtgtta taaacagata atatattatc
     6901 ggtattgttt atttctggat aaaaattgtt ataaatattt tgaactatta ttaataaatc
     6961 tttttcaaaa ttatctttta acacaatacg ttgttgataa tttataatac gacgaataag
     7021 tcttcgcaag acatatcccc tgtcaacatt agaaggatgt aatccatcag caatcaaaaa
     7081 tatagatgct ttaaaatgat cagcaataat tcggaataat ttttcatcat atttttgtga
     7141 gttggtatta atataatcta acataggcat aaaaatatct gtattataaa tcgtattatg
     7201 ggttgtttga aatttaggaa attctgtgac caaaaccatt ctttctaaac ccgctccagt
     7261 atctactcct ttttgtttta atggtgttaa agttttattt ttattcatat aaaattcatt
     7321 aaataccaaa tctcatattt caatattatt tacataaatc tctgtacagg gaccacaagg
     7381 cccttcagat cctactggac cccaaaaatt atcctcttta ttacctttga taaactgttc
     7441 ttcttttatt cctaattttt ttcaaatatc ataagattct tgatcaaatg atatttcact
     7501 atcacccgca aaaacagaga tacttaattt ttcaactgga atattcaaaa cttgtgtaac
     7561 aaattcgaaa gccataggaa tcatttcttg tttaaaatac ccagcgaaag aaaaattgcc
     7621 aagcatttca aacattgtta aatgtttttc atcaccaact aaatcaatat caatggtacg
     7681 caaacatttt tgaatagtaa caatattatt actaccaaga ggtttcataa tattgggatg
     7741 gatatcaagg aatggatttt ttataccaga aaaataatct ttaaattgct gcataccagc
     7801 aacagtaaat aaagcattac tatctccttc gggtaatagc gataccccgg gtatatgaat
     7861 atgattatag tttttaaaaa atttgagaaa tgtatcacga acttcaaatg acttcataat
     7921 aataaattaa atacaaatta ataaataata tttagggata agttgtaata atatacacag
     7981 tatttaaata tggcagctta aaaaagggat ctataaaaat atctggctta tttaaagact
     8041 gctgagatac tatattacta atttgcccca ccaatagacc acgtaaaaat tctttattat
     8101 ccaaagatgt tacaagaggg tcgcctattt taaatttaga tgcatcagaa aagagtgtta
     8161 attttaaaaa tcctttatta tcgcgtttga caagagctac ttcattggat atagaattga
     8221 taacactaat attgatatta ggactatata aagattctac tttggcataa ttatcaaaga
     8281 cttctactac cctacctact aaaactgaat tatctaatgt aatattcata ccgactttga
     8341 cgttatctgc tctcccttta tttataataa atattcctga tgcgccttca atatccgaaa
     8401 tcaaagcatt ggcttggatg gtattatata attgtggttt tacacaaaca gattgtaaat
     8461 ataaattatt agcatttaaa atttgatttg tttttaattg atagtattca ttgcgaatag
     8521 ttctaaaatg aaataagtct tctaaaaaac ctattgggct ctggatattt ttttgagcat
     8581 ttagtgatga aacatacata gttgtggtaa ctttggggct tcaaaaagct caaaattttg
     8641 atttagtagt tagaacacct caaaaaataa taattataaa aaatcataca gaatatcttg
     8701 aaaaaaaagg tttataattc tgtaattttt tattattata tactaacatt atagggtagt
     8761 tttaagagtt aataaatacg gcttcatagc ttcaaaattt tctaaaataa ttccctctcc
     8821 cttaacaaca ctattaacag gatcatctga tatatttact ttcaatcctg tagctttttc
     8881 tattaatgta tccatatctt ttaataaaga agtaccgcct gttagtaaaa tacctttttc
     8941 aatgatatcg gctatcaatt ctggtggagc tatttcaagt gtatctttaa tgatttggac
     9001 tattgtttgt agagattcac tcatagcttc tctaatttcg ctttctgtta ccttgacttc
     9061 ttttggcaaa cctttggcca aatctctgcc acgaattgtc atagtttcat cagtgtttct
     9121 ttctaaagca gaaccaatag taatttttat tttttcagca gtttgctcgc caatagccaa
     9181 tttaaattta ttgcgaacaa aatagattat atcgtcgttt agtttattgc cagcaattct
     9241 aatattttta gtaacaacaa taccgcccaa agaaacaata cttacttcag taatacctcc
     9301 gccaatatca actattaaat tacctcttgc ctcatttaga agaagccctg caccaatagc
     9361 tgtagccaaa gctttttcta ttaaatatac ttctttggcg ccagcatttc taactgcatc
     9421 ttcaactgct ttacgttcaa cttctgtgcc caaagaattt actccaacta ttactttggg
     9481 acctaaaaaa atattttttt ctttaatttt ttgaaacaaa aaacgtaata atttttctgt
     9541 gacttcaaaa tcagatataa caccattgac aataggttta atagcggaaa tgtgactggg
     9601 agtttttcca agcattgctt ctgcttcctt accaatagca ataatttgat ctgtttttgt
     9661 atttaacgcg acaactgcag gctctttgag aacaattcct ttacctttga cataaacaga
     9721 aatttttgta gtgcctaaat ctaaccctat attttggaaa aaactatcag attttttaaa
     9781 aatgttttgt aaaaattgca tattcatata taaaatttaa tatatttatt agtaactttt
     9841 ggcaaataat catcgacgtt ttgctttttt gtgacaataa atacactcgc cagattcatt
     9901 gctttctatt ctatctaatt caagacatct attactggct tttgtttctt gctttattgc
     9961 ttcttcacat tgttcatctt cgcaccaaaa tgctctaaca aacttaccat catttatagc
    10021 ttttttgaat tcgttataat cggagacatc tatggttaaa tcatttttta attttcgtga
    10081 tttctgtaat aaatcattct gaatattcaa aagtaactct ggaattgtat ttaatatatc
    10141 tttgatatca agattacttt tttcaaaatt atctcgtcta acagcagata caacattatt
    10201 gtctaattct ctttgtccta cttctaatcg caaaggaact cctttaattt ctcattcatt
    10261 gattctatat cccaaagtat gcttaaaatc aacatctaat ttagctttga tacctataga
    10321 acttaattgt ttataaatat tttcactaaa ttgaattatt tctttattat cattatctgt
    10381 aattattggt aaaatgacaa tttgaattgg cgcgactttg ggaggtaaaa ttaatccaaa
    10441 atcatcacca tgagctaaaa ttaaacatcc aagagatcta gtagacaaac caaaagaatt
    10501 ttgatagaca tattgttttt ctccgttttc atcaatatat tggatattaa atactttggc
    10561 aaaattatca cctaaatcat gtgaggtaca catttgtata gatttaccat tgggggcaac
    10621 catttcaatt gtatatgtat tcttggcacc agcaaatttt tcttgttggc tttttaaacc
    10681 cacatatcca gatatagcaa aatattgctc ataaaaatct ttatatcaat ttagagcttt
    10741 taaagtcata tccagagttt cttgggctgt gcgaaatgaa gtatgccctt cttgtcataa
    10801 aaattcagtt gttcttaaaa aataataagt ccttttttct gccctaaaaa tattacacca
    10861 ttgattaatt ttgtatggta aatctctata gcttttaatt cacttagata atgctttgtc
    10921 aataatagct tcggaagtgg gacgtaaaac atagggatct aataaatctt catcaccaat
    10981 tgatttaatc ataaagagct caggcgcaaa acctttgata tgttcttttt ctttttcaaa
    11041 taaactcata ggaatgaatg cggggaaata tacattcata ataccatctt ctttaaattg
    11101 tttgtccaat acttgctgaa cactctctca taaggcataa cccaatggtt ttataatcat
    11161 accacctctt gtttcggcat aatctgctac atcagctaat ttaataacat cgttatatca
    11221 atcagcaata ttttcagatt tttttgttaa atctttttta tcaaattttg tctccataat
    11281 ataaaattaa aacaaactaa tgacatcttt tattgtgaca attaaaacta aaatcatcaa
    11341 aaatgcaaag cctcctattg tagcataatt ttcgaatttt ttatttaacg cagatccttt
    11401 aatttgttca aataataaaa ataataattt accaccatca agcgctggaa taggcaataa
    11461 attcataaag gctaaattta tagataagga ggccataaat gcaaagaaaa agccacttcc
    11521 catttttgat attttttgag ttaaaacagc tataccaact gg
//
LOCUS       DAOTJA010000016        11507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6004_length_11507_cov_6.437915, whole genome shotgun sequence.
ACCESSION   DAOTJA010000016 DAOTJA010000000
VERSION     DAOTJA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6004_length_11507_cov_6.437915"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..363)
                     /locus_tag="P5241_01770"
     CDS             complement(37..363)
                     /locus_tag="P5241_01770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV32279.1"
                     /translation="MSHMEICPVFRANLYAIKINTSTCILTLLKNGNPIKKYRVAVGK
                     PSTPTPKGKFHIINKAINPGGPFGARWLGLNIRGYGIHGTNNPSSIGKKVSNGCKGMW
                     GKINNS"
     gene            complement(463..1200)
                     /gene="istB"
                     /locus_tag="P5241_01775"
     CDS             complement(463..1200)
                     /gene="istB"
                     /locus_tag="P5241_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRV32280.1"
                     /translation="MNEEIVNNIRNYCKELKLVTNIVRSIGTIQADSHEEFLAKVLEI
                     EIKHRETTRKNRYLKQAGFDVIKTFENYKFDHIEIPVSLTIDTLKSAEFVKNKENLIL
                     YGPVGTGKTHLAVAVGVAACMNNMKVKFFRTAALFNELIDAINNGIFKKLLRQKEQVD
                     LLICDEWGYIPIDIEGANLLYQVIAGCYEKKSIILTTNLEFSKWNSIFFDEKLTNAIL
                     DRMVHHSHLLIFEGPSWRLQNSLMKYN"
     gene            complement(1197..2717)
                     /gene="istA"
                     /locus_tag="P5241_01780"
     CDS             complement(1197..2717)
                     /gene="istA"
                     /locus_tag="P5241_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRV32281.1"
                     /translation="MTQVKNIKKLYYSKGKKVNEIVKITGHNYRTVMKYLEKTDFNQS
                     LGKQKGDQKGCPKKIDLVIPIINRWLEEDKTAPVKQRHTAKRIYTRLKEEHGELLNVG
                     YRTIADYVAKREKEIYKDEEGYIPLEHPKGEAQVDFGQALFYEKGKQIEGHYLNITFP
                     YSNGGYTQVFKGENQECLLEGMKRIFEYIGHVPYKIWFDNLSAAVILGKNRERNLVEQ
                     FERFSLHYGFEANFCNPNRGHEKGNVENKVGYHRRNLFVPIPHFENIDEYNQELLKLS
                     DIDMKREHYKKGEMILKLLEEEKEFMFKLPEKEFEVGRIKTAITDKYGKAEFETNLYS
                     TSPVYSGEEVVVKATADKVIIMNKDFKTIVIHERIYDKYKESMKWYPYLEALSRRPKA
                     IKYTKFFNELPDVLKDYIDSQDYEGKKRSIKALMKMIEGDEHLGIINATRAIEETLNN
                     GVKDIDSIIATYYRIKNMGNAAINEINLNDNIPRIKEYQSDIDIYDSLYKKQVSAI"
     gene            3064..3426
                     /locus_tag="P5241_01785"
     CDS             3064..3426
                     /locus_tag="P5241_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32282.1"
                     /translation="MVQKVFKKSITVLLLGISLTGVVALAASQSLNNGGATWSGGISD
                     DKVYSKIIDNKVDGLAYKATVWVKNDKGNYNEVTDKTKGMGELYKIYVDIGATHSSFL
                     TPNRAGYKNFEVVKATGN"
     gene            3519..4028
                     /locus_tag="P5241_01790"
     CDS             3519..4028
                     /locus_tag="P5241_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32283.1"
                     /translation="MKKVFNIIGILLGIYLIYTFLIYVNSSEYRLSSDIYDISIYNKS
                     ITCDKLKEYAEKSDTTIVLINFKNTSFFNCDLEFTFINPDKNIKMGRQKSILPGHEIV
                     YKDINDIRAEKVKYFHVIFADEGKVEKIKYLLEQDEYKINFIEKEIKLKLVGRLFLQT
                     LILSFMEHL"
     gene            4203..5417
                     /locus_tag="P5241_01795"
     CDS             4203..5417
                     /locus_tag="P5241_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32284.1"
                     /translation="MFVDITKLNLYINLIIVIYMLLILVFAISAIISIIFVNNINQVQ
                     VIKNKKNNRIVFYTLMAFKTIIVVILLITINTSIEKINLVKHTIFNINKLSKYNFYSV
                     KTSCTPEKELNEKINKMIDSIDTSHFYLYKGDEIGYNKFNKFISSKKLRSYEEKIAVI
                     ISPNMLKYLNVLDENGERVSENRVDETTLLIPLHYKEFENKILEEYNIGDSVAHKYTH
                     EDIIYILDGQVYNSFIHSDMYNYDCIFIFRNVYKDEYYIYGFDFLDEFAAKRLEKGFE
                     DIGINEGSINVIPLKAEYEQRINDLQLALYENISYVFNSFLAFLVCVISLVIIFLELR
                     KKEFGVYKFIGKQPDKTILKFMTMNCVVTIVIAVMINQILVLIIFIEILIYSIYIYMY
                     LKNKAILSLKGE"
     gene            5435..6064
                     /locus_tag="P5241_01800"
     CDS             5435..6064
                     /locus_tag="P5241_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV32285.1"
                     /translation="MIIKLENIKKRYGEKEVLRDFNLEIKKGEFVGIIGKSGAGKSTL
                     LNIIGLLEECDDGKVIIEGDQIKTKDRKKIQSLLKNKIGYLFQNYALIDDLTVYENLK
                     IVLEGKDKEMNKKLILEALNKVELGNILDKKIYQLSGGEQQRVAIARLILKKSEIILA
                     DEPTGSLDRINGKRIMELLRELNNQGKTIIIVTHDEHVIEYCTRVINLS"
     gene            complement(6277..7503)
                     /locus_tag="P5241_01805"
                     /pseudo
     CDS             complement(6277..7503)
                     /locus_tag="P5241_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064321.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            8163..8558
                     /locus_tag="P5241_01810"
     CDS             8163..8558
                     /locus_tag="P5241_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32286.1"
                     /translation="MLKFYLISILIGNSVLWFYELPSIQSGFDYIRFIFVYFIFVLPT
                     ILSYIIFKKLKPSVAKILLILMWVLSGAFIGIGIKDSHGIDEFELSLIFFICFSLSSI
                     FYFLWISFFNKGQNYMNLDSASNAERHWN"
     gene            8650..8793
                     /locus_tag="P5241_01815"
     CDS             8650..8793
                     /locus_tag="P5241_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32287.1"
                     /translation="MNSLQQNQKNLKKKIRLLNIKANFNDTKVSSVATFSYVELFKQI
                     IGL"
     gene            8801..8998
                     /locus_tag="P5241_01820"
     CDS             8801..8998
                     /locus_tag="P5241_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRV32288.1"
                     /translation="MKDSVEEYPEIVGIMQRLGFTEIVKPGMLNTVGRVMTIPKGAML
                     RGFDIEKIKNEFIKNRFEIIE"
     gene            9011..9349
                     /locus_tag="P5241_01825"
     CDS             9011..9349
                     /locus_tag="P5241_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV32289.1"
                     /translation="MSDQFIKNIEHKAIFEMESLVQYQEGTVVSRTLAQSKAHSVTLF
                     AFDKGEEISAHSARGDALVYVLDGEVEIMIGDEKFYLKKGQTIVMPANIPHALFAKER
                     FKMLLIVIFQ"
     gene            complement(9399..9650)
                     /locus_tag="P5241_01830"
     CDS             complement(9399..9650)
                     /locus_tag="P5241_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32290.1"
                     /translation="MHFSLLQMIKLEKRVENVEVQLTKVIEKLDNIEKKIDMEAINAT
                     RHTEIYMKLENMNEDIIFLKHKEHQTEEDLFKLKLKIAK"
     gene            9954..11333
                     /locus_tag="P5241_01835"
     CDS             9954..11333
                     /locus_tag="P5241_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRV32291.1"
                     /translation="MNSLQQNQKNLKKKIRLLNIKANFNDTKVSSVGTFSYVELFKQI
                     IGLSTILEKGVAYKKAANSIFSTAEIIEYLIDANILGFSRFMHIETLRNDEGYKKFKG
                     IDKLPSEKVCRDLLKALPTEALEELRTINKKILDLKSSTEDMREVCINIDDTVITIFG
                     NQEESGIGYNPRYNGRPSFKEKVGFISGTDELLNVTLENGRHHSNYKFLDFFKSCVNQ
                     LPSRYLLKRVRLDRGFFDEENFLYFEDNGIEYVVKCKMYSTVKMIIDFINNNSKDYIW
                     HDISSTLSVMEITVPLPKWQRARRFVIIREKFIPKDINQLMFEECKYKYQAIVTNIDY
                     LTPEEIFQDYNQRCDVENNIDEIKQGYAFSENSLVNHKCNELYLLIKMIAYNIHNWFK
                     QSIMPKEIQHHEISTLRRIFYRVCGNVCGSKYYRYLSFSNNCLLEKIITHIQKQIHIF
                     SRKMITVYT"
BASE COUNT         4139 a         1461 c         1673 g         4234 t
ORIGIN      
        1 gtattagttt ttatttgctt ttttaattat acattattaa ctattattaa tttttcccca
       61 cataccttta catccattag aaactttctt gccaatagag gaagggttat ttgttccgtg
      121 tataccatat cctcttatgt ttaaaccaag ccagcgagca ccgaagggac cacctggatt
      181 gattgcttta tttataatat gaaatttacc cttaggagta ggagtggaag gctttccaac
      241 tgctactctg tattttttta ttggattacc gtttttaagc aatgttaaga tacaggtaga
      301 agtattaatt tttatggcat ataaatttgc acgaaaaact gggcaaattt ccatatgact
      361 cataatataa agtgctttat atagtatatt tgtgttttgc aataattaaa tacaaacttt
      421 tgaaaaaaat ctttgcaaca tccgcattac cataattgta aattaattgt atttcattaa
      481 tgaattttgg agcctccagc ttggcccttc aaatattagt aaatgactgt gatggaccat
      541 cctgtcaagt attgcatttg ttaacttttc atcgaaaaag atactgttcc atttactaaa
      601 ttcaaggttt gtagttaata ttatactctt tttctcatag catcctgcta taacctggta
      661 taataggttg gctccttcaa tgtcaattgg tatgtagccc cattcatcgc atatcaatag
      721 atctacctgc tctttctgtc ttaataattt cttaaatatc ccattattta ttgcatctat
      781 taattcatta aaaagtgcag ctgttctgaa gaacttaacc ttcatattgt tcatacaagc
      841 agcaactcca actgctactg caagatgtgt tttgcctgta cctactggtc catatagtat
      901 taaattctct ttgttcttca caaattctgc acttttaagt gtatctattg ttaaactaac
      961 tggtatttca atatggtcaa atttgtagtt ttcaaaggtc ttaataacat caaatcctgc
     1021 ttgctttaaa tatctatttt ttcttgtagt ttctctatgt tttatttcta tttcaagcac
     1081 tttagcaaga aattcttcat ggctatcggc ctgtatagtt cctatgcttc taacaatatt
     1141 tgtcacaagt ttaagctctt tgcagtaatt ccttatgtta tttactattt cttcgttcat
     1201 attgcagaca cctgcttttt gtacaggcta tcatatatgt cgatgtctga ttgatattct
     1261 ttaatcctcg ggatattgtc attaagattt atttcattta ttgcagcatt tcccatattt
     1321 ttgattcgat agtaagttgc aataatactg tctatgtctt taacaccatt atttaaggtt
     1381 tcttctatag ctcttgttgc atttattatc cctaaatgtt catctccctc tatcattttc
     1441 ataagtgctt taatacttcg cttttttcct tcataatctt gactatctat atagtccttc
     1501 aaaacatcag gtaactcgtt gaaaaacttt gtatacttta ttgctttagg ccttcttgat
     1561 aaggcttcaa ggtaaggata ccacttcatc gattctttat atttatcata aattctttcg
     1621 tgaattacta ttgttttaaa gtctttattc attattatta ctttgtcagc tgtagctttc
     1681 acaacaactt cttcaccaga ataaactggt gatgttgaat aaagattagt ttcaaattct
     1741 gccttcccat acttgtctgt tattgcagtt tttattctac ctacctcaaa ttccttttca
     1801 ggaagcttaa acataaattc tttttcttcc tcaagaagct ttaaaatcat ttctcctttt
     1861 ttgtagtgct ctcttttcat atcaatatcc gaaagcttca gtaattcctg attgtattca
     1921 tcaatgtttt caaaatgtgg aattggaaca aacaaatttc ttctatgata tcctacttta
     1981 ttttcaacat ttcccttttc gtgtcctcta tttggattgc aaaaattagc ttcaaatcca
     2041 taatgcagtg agaatctctc aaattgttca accaggtttc tctctctatt tttacccagt
     2101 attactgcag cggaaagatt gtcaaaccat attttatatg gaacatgacc tatgtattca
     2161 aaaattcttt tcatcccttc taaaagacat tcttgatttt ctcccttaaa aacctgagta
     2221 tagccgccat tgctgtatgg aaaagttata tttaaataat gtccttcaat ttgctttcct
     2281 ttttcataaa acaaagcttg tccaaaatca acttgtgctt cacctttagg atgttcaaga
     2341 ggaatatatc cttcttcatc cttgtagatt tctttctccc tctttgcaac ataatctgct
     2401 attgttctat aacctacatt tagaagttct ccatgttctt cttttaacct cgtataaatt
     2461 cttttagctg tatgtctttg tttaactgga gccgttttat cttcctctaa ccatctatta
     2521 attatcggta taacgaggtc aatcttttta ggacaacctt tttggtctcc cttttgttta
     2581 cctaaacttt gattaaaatc ggttttttca aggtatttca tcactgttct gtaattatgt
     2641 ccagtaattt taacaatttc atttactttt ttccctttac tgtaatacaa ttttttgata
     2701 ttcttaactt gagtcattgt taacattcct ttctaccccc tttagcgtcg tcaactaaag
     2761 ggtaggatat tttgaggatg ttggcaatga cttttttcca tttttttatt tgtattttat
     2821 ttctgcaact ctttgtattt atattttaca tcaaacatat ttgctcaaaa aagttaagtt
     2881 gcttaatttt gctaatacaa aacatgttat tctcctcctc tgtggatagt tttagatatt
     2941 tgtataattt aataatgcca cagtggagga aaatataaaa gccttacggt taaataaata
     3001 gtaaaatcat tggttgcaaa attctgtaac cgactatata atacaaaaat aaggaggtta
     3061 aaaatggtac aaaaggtatt taaaaagtca attacagttt tattattagg catatcttta
     3121 acaggtgtgg ttgctttagc agcatctcaa tcattaaata atggtggagc aacatggtca
     3181 ggaggaatat cagacgacaa ggtatattca aagattattg ataataaagt ggatggattg
     3241 gcatataaag ctactgtttg ggtaaaaaat gataaaggta attataatga agttacagat
     3301 aaaacaaaag gtatgggtga attatataaa atatatgttg atataggggc aacacatagt
     3361 agtttcttaa cacctaaccg agcaggatat aagaattttg aggtagtgaa ggcaactgga
     3421 aattaaatgt atttaacatt atcaaaatcc aatgcattaa tttgcattgg attttgataa
     3481 taaaattcat aaagctatac agggggataa aatattatat gaaaaaggta tttaatataa
     3541 ttggtatatt attaggaata tatttaattt atacatttct aatatatgtt aattcttcag
     3601 aatatcgttt aagtagtgat atatatgata tttctattta taacaagagt ataacatgtg
     3661 ataaattaaa ggaatatgca gaaaaaagcg atacaacaat agtattaatt aattttaaaa
     3721 atacaagttt ttttaattgt gatcttgagt ttacatttat taatccagat aaaaatatta
     3781 aaatgggtag acaaaaaagt attttaccag gtcacgaaat tgtatataaa gatattaatg
     3841 atattagagc agaaaaagtg aaatattttc atgtgatttt tgctgacgaa ggaaaagttg
     3901 agaagataaa atatttatta gaacaagatg aatataaaat taattttatt gaaaaagaga
     3961 taaaattaaa attagttggt cgtttatttt tacagacttt aatattaagt tttatggaac
     4021 acttatgatg ttattgtttt taagcatagt aatgtattat gtacatagat taaaggaaat
     4081 tggaatttta aagttaaacg gttggagtga cagaaaaatt atttcaaggc ttttttttta
     4141 gaatgtatgg tcaaataatg ctgtttagct tgatattaat catacttttt ggtatttata
     4201 ttatgtttgt tgatataaca aaattaaatt tatatattaa tcttataata gttatatata
     4261 tgctattgat tttggttttt gctatatctg caattataag cattattttt gtaaataata
     4321 ttaatcaggt tcaggttata aaaaacaaaa aaaataatag aatagtattt tacactttaa
     4381 tggcatttaa gactattatt gtagtaatat tattaataac aattaataca tcaatagaaa
     4441 aaataaattt ggttaagcat actatattta atattaataa attaagtaaa tataattttt
     4501 atagtgtaaa aacttcatgt accccagaaa aagagttaaa tgagaagatt aataaaatga
     4561 tagattctat tgatactagt catttttatt tatataaggg agatgagata ggatataata
     4621 aatttaataa attcatttct agtaaaaaac ttagaagtta tgaagaaaaa attgcagtta
     4681 taatttcacc taatatgtta aaatatttaa atgttttaga tgaaaatgga gaaagagttt
     4741 cagaaaatag agttgatgaa actacattat taataccatt gcattacaaa gagtttgaaa
     4801 ataaaatttt agaagaatat aatataggag atagtgtagc acacaaatat acacatgaag
     4861 acattatata cattctagat ggacaagtat ataattcatt tatacattca gatatgtata
     4921 attatgattg tatttttatt tttagaaatg tttataaaga tgaatattat atatatggat
     4981 ttgatttttt agatgaattt gctgcaaaaa gacttgaaaa gggatttgaa gacattggaa
     5041 taaatgaggg tagtataaat gttattccgc ttaaagctga atacgaacaa agaattaatg
     5101 atttacaatt ggctctttat gaaaacattt cttatgtgtt caatagtttt ttagcatttt
     5161 tggtatgtgt aatatcatta gtaattattt ttcttgaact aagaaaaaaa gaatttggtg
     5221 tatataaatt tattggtaaa caacctgata aaacaatatt aaagtttatg acaatgaatt
     5281 gtgtagttac aatagttatt gcagtaatga taaatcagat acttgtatta ataatattta
     5341 ttgaaatttt aatatacagc atatatatat atatgtattt gaagaataaa gcgatattat
     5401 cattaaaagg tgaatagttt gggaggggaa catagtgatt attaagttgg aaaatataaa
     5461 aaaaagatat ggagaaaaag aagttttaag agattttaat ttagaaataa aaaaaggtga
     5521 gtttgttggt ataataggaa aaagtggagc tgggaaatca acattattga atattatagg
     5581 attattagag gaatgtgatg atggtaaagt aataattgaa ggagatcaga taaaaacaaa
     5641 agatagaaaa aaaatacaaa gcttattgaa aaataaaata gggtatttat ttcaaaatta
     5701 tgccctaata gatgatttaa cggtgtatga aaatctaaaa atagtattag agggtaaaga
     5761 taaagaaatg aataaaaaat taattttaga agctttaaac aaagttgaat taggaaatat
     5821 tttagataaa aaaatttatc aattatctgg aggagaacag caaagagtag caatagctag
     5881 attgatatta aaaaaaagtg aaataatttt agcagatgag cctacaggaa gtttagatag
     5941 aataaatgga aaaagaataa tggaattgct tagagagtta aacaatcaag gtaagacaat
     6001 tattattgtt actcatgatg agcatgtaat tgaatattgt acacgagtga taaatttatc
     6061 ataaaaatat ccttcgagaa catccgtaga tttgtgtaaa tttatttaga tatgattaaa
     6121 ataaactgga aattactatg ggagttaaat cctgtagtaa tttttatttt tacgggaatt
     6181 actaaataat gagatagtgt aattgttgta cagtattatt ttaaatctat tttttatttt
     6241 tatacatttt ttctaaatta gtacaagcta ttaaatttaa atatattgtt caagcctatc
     6301 accatacatt atacttagtt gtgctaatgt actatgccag tttggagaaa gggaagtcca
     6361 cttagccatt atttgctctg tagctagata caaaaatttt cttaaagaat catccgtagg
     6421 aaaagatgtt ttaactttag taaactttct taactgtcta ttaaaacctt caagagcatt
     6481 tgtagtatag ataatccttc taatttccgg aggaaaatta aaatatgtag acaagttatt
     6541 ccagttgtta gtccaagatt gtattacaat ggcatatttt gttccccacg tactctcaag
     6601 tgactctaaa gctttaattg ccatttgttc attagtagct tggtatactt gctttaaatc
     6661 cttcataaaa gctttactat cttttgaagc tatatacttt aatgagtttc gtatttgatg
     6721 tattatacag ttttgtacat tcacttgtgg aaatactgct tttatggcat caggaagtcc
     6781 cttaagacca tccatacaag ctattaatat atctttaaca ccacggtttt ttaggtcatt
     6841 acatacagat agccaaaact tagcgccttc ctgttctcct atctagatac caagtatatc
     6901 tttatatcct ctcatatcta aagccataca tatataagca gctttattaa caatttgatg
     6961 ttcttccttg actttatagt gtatagcatc catatataca attggataga taggttctaa
     7021 tggcctgttt tgccaagcaa tagctgtttc catcacttta tcagttattt ttgaaatcat
     7081 agctggtgat atatccaccc catatagttc atcaatttct gatttaatat catctacaga
     7141 catacctcta gcatagagtc ctataacttt tttatcaagt tcattacaca cagtttcata
     7201 ttttttcact atccttggtt caaattcagc ttttctatcc ctaggaacat tgatatcaac
     7261 ttcaccaaag ctacttttaa tcttctttgt actataccca tttctataat ttttctcatc
     7321 atctaagcta cgttcatact tctctcttcc taagtgttcc tccatctcag cctctaaaaa
     7381 ctgttgaata atgtttccaa ataacctctg cataagacca ttctttccaa ctaagtcttc
     7441 catgctctta cactttttta attctgcatt gaggtcaata ttaggaatat ttaattcagg
     7501 cataaatatc aactcctttc aaaatttcta ttagttatta tttgcatatt tttcagttac
     7561 acatacataa aaaagaacat ccatagatgt gatttacaca aaactatgga tgcttcctaa
     7621 atttttgagg tagttgataa attaatttta aaaaatatga tggaatattt tgtcgaaatt
     7681 atttacaaga tattcaaaag tagtataata aatattaaga ataatttaat atatggatta
     7741 gaatgtttga gtaggtgcta aagattaatt tggttaataa gctatgttaa atacaaattt
     7801 ggaacttctt tttcatgatt aatgtgcact tttaaagtga aacacaattt ggtgacaagt
     7861 ttaattatta tgtgctatag agcattttta aaaaaaattg gagaataatt caggtaaaat
     7921 ttttaatttt aaaatataat tgcaaagcta atattgattt tgtccaaact atagagatag
     7981 tgattgttat acaaagaata tgagatttta tttaataatt tgcatataat ttcatctctc
     8041 cctcggaggt attttttgct atatataaga taactcaaag gggagatgaa attcaaatat
     8101 catataactt tacaaaactt aataattatt ttggaggtgt taaaatcaat aagggaaaga
     8161 aagtgttaaa attttattta attagtattt taatagggaa ttcagttctt tggttctatg
     8221 aactaccaag tatacaaagt ggttttgact atattcgatt tatttttgta tattttattt
     8281 ttgtattacc tacaattcta tcttatatta tttttaaaaa attaaagccc agtgtagcaa
     8341 aaattttgtt aattttaatg tgggttttat caggagcatt cattggtatt ggtattaagg
     8401 attcccatgg aatagatgaa tttgaacttt cacttatatt ttttatatgt ttttcattaa
     8461 gttctatttt ctatttttta tggattagtt tctttaataa aggtcaaaat tatatgaact
     8521 tagattcggc aagtaatgcc gaaagacatt ggaattaaag gattttttgt tcaaaaaaat
     8581 taaaagaaat catccctttt aagtgttaaa atataattac aaaaaacaaa acacaaaagg
     8641 gtgatttcta tgaatagttt acaacaaaat caaaaaaatt taaagaagaa aattagactt
     8701 ttaaacataa aggcaaattt taatgatacg aaagtttctt ctgttgcaac tttttcttat
     8761 gtggaattgt ttaaacaaat aataggacta taaaaggaac ttgaaagatt cggttgaaga
     8821 atatcccgaa attgttggaa taatgcaaag gcttggtttt actgagatag taaagccagg
     8881 tatgttaaac acagtaggta gagtaatgac aatacctaag ggagcaatgc taagagggtt
     8941 tgacatagaa aaaataaaaa atgaatttat taaaaataga tttgaaataa tagaatagga
     9001 gggattttta atgagcgatc aatttataaa aaacattgag cataaggcaa tatttgaaat
     9061 ggaaagtctt gttcaatatc aagaggggac ggttgttagt agaaccttag cccaaagcaa
     9121 agcccacagt gttactctat ttgcctttga caagggggag gaaatttcag cccattctgc
     9181 tcggggagat gcccttgtat atgttttaga tggtgaagta gaaattatga ttggagatga
     9241 aaagttttac ttaaagaagg gccaaacaat agtaatgcca gccaatattc cccatgccct
     9301 ctttgcaaag gaaaggttta aaatgcttct tatagtgatt ttccaataat catttaaaaa
     9361 agggttgttt caagattttg agacaaccct tttaaaaatt attttgcaat tttaagcttt
     9421 agtttaaata ggtcttcttc agtttgatgc tctttatgct taaggaaaat tatatcttca
     9481 ttcatatttt ccagcttcat ataaatttca gtatgtcttg tagcgtttat ggcttccata
     9541 tcaattttct tttcaatatt atcgagtttt tcaatgactt ttgtaagttg aacttctaca
     9601 ttttctactc ttttctctaa ttttatcatt tgaagtaaag aaaagtgcaa aagtgttaaa
     9661 aacaattttc agaattttca caaccattca aaattggaaa caatatatat acctatataa
     9721 tagtataact atagttatat atacaataac tattagtata tacatatata tagttaatat
     9781 aaacatagta gtagttagta ataggtaaag ttatatttag atcttggatt cggcaagtaa
     9841 tgccgaaaga cattggaatt aaaggatttt ttgttcaaaa aaattaaaag aaatcatccc
     9901 ttttaagtgt taaaatataa ttacaaaaaa caaaacacaa aagggtgatt tctatgaata
     9961 gtttacaaca aaatcaaaaa aatttaaaga agaaaattag acttttaaac ataaaggcaa
    10021 attttaatga tacgaaagtt tcttctgtag gaactttttc ttatgtggaa ttgtttaaac
    10081 aaataatagg actatcaact attttagaaa aaggcgtagc ctataagaag gcagctaatt
    10141 ctatttttag tactgcagaa attatagaat acttgattga tgcaaatata cttgggttct
    10201 caagatttat gcatattgaa accttaagaa atgatgaagg atataaaaag tttaaaggta
    10261 tagataaatt acccagtgaa aaagtatgta gagacctttt aaaagcttta ccaacagaag
    10321 ctttagaaga attaagaact ataaataaaa aaatacttga tttaaagtct tcgactgaag
    10381 atatgcgaga ggtttgtatt aatattgatg atactgtgat aactatcttt ggcaatcaag
    10441 aagaatcagg aataggatat aatccaaggt acaatggaag accatcattt aaagaaaaag
    10501 ttgggtttat ttcaggaact gatgaattac ttaacgttac tcttgaaaat ggtagacatc
    10561 acagtaatta taaattttta gatttcttta agagttgtgt aaaccagctt ccttcaagat
    10621 atcttcttaa aagagttaga cttgatagag gtttttttga tgaagaaaac tttttatact
    10681 ttgaagataa tggaatagaa tatgttgtta aatgtaaaat gtattctact gttaaaatga
    10741 taatagattt tataaataat aattcaaagg attatatatg gcacgatata agttccactt
    10801 tatctgttat ggaaattact gttccattgc ctaaatggca aagagcaaga agatttgtta
    10861 taataagaga aaaatttatt ccaaaagata taaatcaatt aatgttcgaa gaatgtaaat
    10921 ataaatatca agcaatagta acaaatatag attatttaac acctgaagaa atatttcaag
    10981 attataatca acgatgtgat gttgaaaata acatagatga aataaaacaa ggctatgcct
    11041 ttagcgaaaa tagtttagta aatcataaat gcaatgaatt gtacctttta ataaaaatga
    11101 tagcatataa cattcataac tggtttaaac agtcaattat gcctaaggaa atacaacatc
    11161 atgaaattag tacattaaga agaatatttt atagagtttg tggtaatgtt tgcggcagca
    11221 aatattatag atatttaagt ttttcaaata attgcttatt agaaaaaatc attactcata
    11281 ttcaaaaaca aatacatata tttagtagaa aaatgataac agtttatact taatagtaaa
    11341 ataaaaacaa taaattacct taaaaatcca aatgttattt gggtttatta agtatactca
    11401 aaaaaagacc taacggtata aaatatcata ataaatgtaa atttattcat aaagaaaaca
    11461 aaaatggcaa gtaaaaactt ttttaaaatt tacttgccga attcaag
//
LOCUS       DAOTJA010000017        11501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6009_length_11501_cov_5.457889, whole genome shotgun sequence.
ACCESSION   DAOTJA010000017 DAOTJA010000000
VERSION     DAOTJA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6009_length_11501_cov_5.457889"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            434..715
                     /locus_tag="P5241_01840"
     CDS             434..715
                     /locus_tag="P5241_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32292.1"
                     /translation="MAINTSNIQFNESKTTQTTWPKGFLILTIVLLCCVIASVFVIQF
                     LSKQSSKTLAQLDQDIEVEKQVFSSDTSNELANFQNELYNVQILLKNHV"
     gene            743..973
                     /locus_tag="P5241_01845"
     CDS             743..973
                     /locus_tag="P5241_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32293.1"
                     /translation="MNTLPQVAFQNLDIDYNTKQIEIQGVTQNNEMVAKATYVFSHMD
                     GVKQANLKNVKKDISGVSFVIVLDVSDTFFKQ"
     gene            999..1613
                     /gene="pilO"
                     /locus_tag="P5241_01850"
     CDS             999..1613
                     /gene="pilO"
                     /locus_tag="P5241_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRV32294.1"
                     /translation="MKETTKQTVAILSITVLLGVSIFVFLKFTLPAFDNFIALKTQIT
                     EKQQIKAELTRRKQVVMDIYSKYQTIGADLDRIRLALPSDPQLAEVLSSLNTIASTSN
                     IVLGDISFRELSPVKTTSKTKNQVPYTTIEVTINVEGSFVNIQTFVQEIEREIRIMDI
                     KQLIIHSVMMTPQLSSNKTTSKTKNYNIPSLLKTNIILHTYYQP"
     gene            1632..1934
                     /locus_tag="P5241_01855"
     CDS             1632..1934
                     /locus_tag="P5241_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32295.1"
                     /translation="MAIQIQQQKKKNPILLILLIVVVIIFVGFKIIQKLNITKIINIS
                     QSGTKELADIKNTENIDNIMLDINRVLQDGVFQQLVSHSSLDVIIDMIGKEDPFSR"
     gene            1981..3720
                     /locus_tag="P5241_01860"
     CDS             1981..3720
                     /locus_tag="P5241_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRV32296.1"
                     /translation="MIQEDILLEALKQKGYKLDSKELKEETQLFHKSLEELLVEKNLI
                     AEDALAVVKGEILNLPVKIFDKKEVVDKNILNIIEESAARNYKVAVFNKDNDYVYIAA
                     LYPEDNKCFEAMNFIAKQLGVKLKLYVTTYSGLQRIWRNYGNFAENLANILKELQQKI
                     GLQLKQGSSAQNIVNFKTIESTLVEEAPIIKLISTILTEAVRLGASDVHFEPTQRVLR
                     VRFRVDGVLYTAVLLPLDIHSAIISRIKIMSSLQIDEMRIPQDGRFRTIIDEKPIDFR
                     VSTFPTSYGEKVAIRVLDPTVGLLGVHQLGLNNYEINKVNTAIARPYGMILVSGPTGS
                     GKTTTLYALLQKLNKDADNIVTLEDPVEYTLEGINQSQVLPEINYTFARGLRHIVRQD
                     PDIIMVGEIRDSETAELAIHAALTGHLVLSTIHTNNAIGVIPRLLDLGIQKFLIPSTL
                     NMVISQRLVRKLCPLCKKEREANIEETQIIDEALKNMPKELIGNLGARPYKIFDPVGC
                     SECNNKGYKGRVGLFEVLEMSPQMENIILGNMSETQLQVEAQKAGMITLRQDAILKVL
                     SGTTSIEEAISET"
     gene            3820..4893
                     /locus_tag="P5241_01865"
     CDS             3820..4893
                     /locus_tag="P5241_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRV32297.1"
                     /translation="MEENKHSQKLVDLLFLAAKQGASDLHLSIGKKPIIRIDGKLIII
                     NKEEIITPELMADYIAAILTDEQEEMLLHNKEIDFAFSLEDKIRFRSNIYFQRGYLAA
                     AFRLIPMHIRTIEELNLPPILHSFTNYNQGLILVVGPAGHGKTTTLAAMIDEINHKRM
                     DHIITIEDPIEYTFVADRALISQREVGGDTLNFNNGLRASLRQDPDVIMIGEMRDPES
                     IAIALTAAETGHLVFSTLHTNSAVQTIDRIIDSFPATQQSQIRSQLSAVLVGIVSERL
                     VPRIQGGRIPACELLIMNTAARSLIRENKTYQIDLVVETGLKEGMMTLNRALSDLVRR
                     REISMETAKLYSLNPEELRSLLI"
     gene            5056..5811
                     /locus_tag="P5241_01870"
     CDS             5056..5811
                     /locus_tag="P5241_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV32298.1"
                     /translation="MEFIYKAKDLKGAEKIGKIEARSEDLAVQLLQGYGLIVYDLKAV
                     ENQGIFDKLFGKKKHIGTKELSLFLRQFSTLLSSKVPLMDSLKTLLAQTNSSALKDMI
                     FNLISGIDAGLSLSQAMSRESNIFSSFYIEMVRSGEISGRLEEVFNYLADYAENEANL
                     NTKAKSAMIYPIFIIVIFLLVGTIISISVAPQMMDIFQEFGQNPPLLTRILIGFGQFL
                     MDWGIIVGVIIVGVIIAMINYFKTKEGQNVLSF"
     gene            5815..6264
                     /locus_tag="P5241_01875"
     CDS             5815..6264
                     /locus_tag="P5241_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV32299.1"
                     /translation="MKIPIIGNIYKAIYISRFAESFGTLLHGGIPVVQALEVAGSATG
                     NFVFDAIGKEVAEGVRRGEPISKLLQNYPEYFPPLVSQMVYVGENTGRLDTLLKRISV
                     YYFNEVENGFKVLLEMIQPVLIVVIAIFVGILIAGVLLPIYQLAEVA"
     gene            6375..6947
                     /locus_tag="P5241_01880"
                     /pseudo
     CDS             6375..6947
                     /locus_tag="P5241_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
     gene            7532..7825
                     /locus_tag="P5241_01885"
     CDS             7532..7825
                     /locus_tag="P5241_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32300.1"
                     /translation="MGSVAQRKKEAFTQHDSYDITLRQRNENEVATIMDIENVLDKFL
                     RAKLVAAKGDKTKMSKIDKLLDRVSDDAIEIFNTAKHINKHYDKSTIKEAFYK"
     gene            8015..8500
                     /locus_tag="P5241_01890"
                     /pseudo
     CDS             8015..8500
                     /locus_tag="P5241_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8525..8878
                     /locus_tag="P5241_01895"
                     /pseudo
     CDS             8525..8878
                     /locus_tag="P5241_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
     assembly_gap    9107..9206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9207..9502
                     /locus_tag="P5241_01900"
     CDS             <9207..9502
                     /locus_tag="P5241_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32301.1"
                     /translation="TGNIVSSSGDVKGARLCIGDNCQDSWTSGGSVPTGGMIFSDNQN
                     NPNLLSAGYLNTGKLINYVECVNGQFNPNGECICDICCCQHYSEFLYVYQKLP"
     gene            9512..10093
                     /locus_tag="P5241_01905"
     CDS             9512..10093
                     /locus_tag="P5241_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRV32302.1"
                     /translation="MNCDSIVIGTATFDTFLKGFKYEIINSNVYANGKALAIAYGSKE
                     KVEIIDHQAGGGAVNIALCLARLRLKTNIIIRICKDYEGDFILNTLKSQGNIFTNYIQ
                     FDRNIKTASSCVLVTGDGERTILSYKGCGQNLVIDSKILNKIKTHMLVASTIAGNSTN
                     FQAIFEYKKQHPETILIANPGVADIEILRKHKE"
     gene            10187..10474
                     /locus_tag="P5241_01910"
     CDS             10187..10474
                     /locus_tag="P5241_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV32303.1"
                     /translation="MDKYVEGICIMTMGADGLIFSDRNNIYTAKNSEDVVVVDSTGAG
                     DAFVSGFTAGYYQKHDPIYAVKFGLANSSQVIEKIGANIGSLSLADLKLER"
     gene            10534..11241
                     /locus_tag="P5241_01915"
                     /pseudo
     CDS             10534..11241
                     /locus_tag="P5241_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
BASE COUNT         4255 a         1284 c         2036 g         3826 t
ORIGIN      
        1 gagagattta cgtattcaag ttattaaagg ggatccattt tctttaaaca ttgtaaatta
       61 cgataaaata atagaacctg ttataaaaga aataggcact agtttatctt gcgcatgtgg
      121 tttggcatta agagaattta aataattata ttatacaaat tatatcaaac aacaactcta
      181 ttattttaag atattcttag agttatgact acaattagat tagatagtta ttttatatat
      241 aataactaga caaaagtaaa aaatgtttaa aacacttgca ttttttaaaa aaatgctata
      301 taattatata tatagatttg taatatagat tatatatacg aatttctcga tgaattagat
      361 attataaatc gaataatata caatagctta atcagataat tcgatttatg taatatattt
      421 aatatattgt aaaatggcaa taaatacttc aaatatacaa tttaatgagt ctaaaactac
      481 ccaaactact tggccaaaag ggtttttaat tttaacaatt gttctattat gctgtgtaat
      541 agcgagtgtt tttgttattc aatttttgtc taaacaaagt tctaaaacgt tagctcaatt
      601 agaccaagat atagaggtag agaaacaagt tttttcttcc gatacttcta acgagctggc
      661 aaatttccaa aatgaattat acaatgtcca gatcctatta aaaaatcatg tttgagtttc
      721 tatcgctctt acaaaaatag ctatgaatac tttgccacaa gttgcttttc aaaacttaga
      781 tatagattat aatacaaaac aaatagaaat tcaaggtgtg actcaaaata atgaaatggt
      841 tgctaaggcg acatatgtat tttctcatat ggatggtgta aaacaagcaa atttaaaaaa
      901 tgttaagaaa gatatttctg gggtgtcttt tgttattgtg ttagatgtta gtgatacatt
      961 ttttaaacaa taaataaatt tattagtaaa tttgttttat gaaagaaaca actaaacaaa
     1021 ctgtagcaat attatcaatt actgttcttt tgggggtgag tatatttgtg ttcttgaaat
     1081 ttaccttacc tgcttttgat aattttatag ctttgaaaac ccaaattact gaaaaacaac
     1141 agattaaggc agaactcaca aggcgtaagc aagttgttat ggatatttat agtaaatatc
     1201 aaactatagg tgctgattta gatagaataa gattggcctt accttctgat ccacaattgg
     1261 ctgaagtttt atcttctttg aatacaatag caagtacaag taatatagtt ttgggtgata
     1321 taagttttag agagctatca cctgtaaaaa caactagtaa aacaaaaaat caggttcctt
     1381 atactactat tgaagtaaca attaatgtgg aagggtcttt tgtaaatatt cagacatttg
     1441 tgcaagaaat agaaagagag atacgcatta tggatattaa gcaattaatt atccattcag
     1501 taatgatgac gcctcaactt agttccaata aaactaccag caaaactaaa aattataata
     1561 taccttcatt attaaaaaca aatataattt tacatacata ttatcaacct taatatttta
     1621 actatattgt aatggctatt caaatacaac aacaaaaaaa gaaaaatcca attttattaa
     1681 tattattgat tgtagtggta attatttttg ttggttttaa aataatccaa aaattaaata
     1741 tcaccaaaat tataaatata tctcagtctg gtactaaaga gttagctgat attaaaaata
     1801 cagaaaatat agacaatatt atgttagata ttaatagggt gttacaagat ggagtgtttc
     1861 aacaattagt atcacatagt tcgcttgatg taataataga tatgataggg aaagaagatc
     1921 cgttttctcg ataggaattt attatatttg tgttttgtat gttattttta aaataatttt
     1981 atgattcaag aagatatttt attagaagcg ttaaaacaaa aaggctataa attagactcc
     2041 aaagaattga aagaggaaac acaattattt cataaaagtt tagaggaact tttagtagaa
     2101 aaaaatttga tagccgaaga tgccttagct gtagtaaagg gcgaaatttt aaatttacct
     2161 gttaaaattt ttgataaaaa agaagttgtt gataaaaata ttttgaatat tatagaagaa
     2221 agtgctgcta gaaattataa ggtggcagtg tttaataaag ataacgatta tgtttatata
     2281 gctgctttat atcctgagga caacaaatgt tttgaagcaa tgaattttat tgctaaacaa
     2341 ttgggagtaa aattaaaatt atatgttact acttatagtg gcttacagag aatttggaga
     2401 aattatggta attttgctga aaatctggca aatattttaa aggaattaca gcaaaaaata
     2461 gggttacaat tgaaacaagg aagttcagca caaaatattg tgaattttaa aacaatagaa
     2521 agtactttgg tagaagaagc accaattatt aaactaatta gtactatttt aacagaagct
     2581 gtcaggttgg gcgcttcaga tgtacatttt gaaccaacac aaagggtgtt gcgagtgaga
     2641 ttccgtgttg atggagtttt atatacagcc gtattattac cattagatat tcattcagca
     2701 attatttcta gaattaaaat tatgtctagt ttacaaattg atgaaatgcg tataccacaa
     2761 gacggcaggt ttcgtacaat tattgatgaa aaaccaattg attttcgtgt atctactttt
     2821 ccaacaagct atggagaaaa ggtggctata agagttttgg atccaactgt tggattgttg
     2881 ggagtgcatc agttaggttt aaataattat gaaataaata aagttaatac agctatcgct
     2941 cgaccttatg gtatgatttt agtgtctgga ccaacaggtt ctggtaaaac tactacatta
     3001 tatgcgttat tacagaaatt aaataaagat gctgataata ttgttacgtt agaagatcct
     3061 gtagaatata cattagaagg gattaatcaa tcgcaagttt taccagaaat aaactatact
     3121 tttgcaagag gtctcagaca tattgtccgt caggatcctg atataataat ggtaggtgaa
     3181 attcgtgatt ccgaaacagc tgaactggcg attcatgcag cgctaactgg acatttagta
     3241 ttatcgacta tccatactaa taatgctata ggagtaatac caagattatt agatctgggt
     3301 atacagaagt ttttgattcc ttctacattg aatatggtaa tatcacaaag attagtgcgt
     3361 aaactttgtc cattatgtaa aaaagaaaga gaagcaaata tagaagagac acaaattatt
     3421 gatgaagctt taaaaaatat gccaaaagaa ttaattggca atttaggtgc tagaccttat
     3481 aaaatttttg atccagtagg ttgttcagaa tgtaataata aagggtataa agggagagtg
     3541 ggattatttg aagttttgga aatgtcgcca caaatggaaa atattatatt gggtaatatg
     3601 tcagaaacac aattacaagt agaggctcaa aaagcaggta tgattacatt gcgacaggat
     3661 gcaattttga aagtgttgag tgggactact tctatagaag aagcaattag tgaaacttaa
     3721 ttagagtatt agagttgtta atggcttttt taaaaaagcc attaaacact aattcataat
     3781 ttaataaaac tacaattaaa atatattaaa tttattattg tggaagaaaa taaacattct
     3841 caaaaattag tagacctttt atttcttgct gctaaacaag gagcatcaga tttacatttg
     3901 agtattggca aaaaacctat tataagaata gatggtaagc ttattataat aaataaagaa
     3961 gaaattatta ctccagaatt aatggctgat tatattgcag ctattttgac agatgagcaa
     4021 gaagaaatgc tattgcataa taaagaaatc gattttgctt tttctttaga agataaaatt
     4081 cgttttcgtt ctaatattta tttccagcgt gggtatttag ccgcagcttt tcgtttgatc
     4141 cctatgcata ttagaactat tgaagaatta aatttgcctc caattttaca tagttttact
     4201 aattacaatc agggattaat tttggtagta ggaccagctg gccatggtaa aacaactact
     4261 ttggcagcaa tgattgatga aattaatcac aagcgcatgg atcatattat aacaattgaa
     4321 gatcctattg aatatacttt tgtagctgat agagcgttaa ttagccaaag agaagtaggt
     4381 ggagatactt taaattttaa taatggatta agagcatctt tgcgtcaaga tccagatgta
     4441 attatgatag gtgaaatgcg tgatccagaa tctattgcta ttgccctaac agctgcggaa
     4501 acaggacatt tggtattttc gactttgcat actaattctg cggtacaaac tattgatcgt
     4561 attattgata gttttccagc aactcaacaa tcacaaataa ggtcacaatt atcagcggtg
     4621 ttagttggta tagtttcaga gcgtttagtc ccacgtattc aaggtggacg aattcctgct
     4681 tgtgagcttt tgataatgaa tacagcagct cgcagtttaa ttcgtgaaaa caaaacttat
     4741 caaattgatt tagtagtaga aactggatta aaagaaggaa tgatgacttt gaatagagct
     4801 ttgtcagatt tagttagaag aagagaaata tctatggaaa cagcgaaatt gtattctttg
     4861 aaccctgaag aactacgaag tttgcttatt taaattaaga accaaaatac aaaatgacaa
     4921 atcaaaatta aaaaacgact ttaataaata tattttttaa acttattaac ttttgaggag
     4981 aaaaaataat tttgacattt gatatgtatt tttaatattt aatctttaat ctaattttta
     5041 atatttaatt taactatgga gtttatttat aaagcaaaag atctaaaagg agcagaaaaa
     5101 attggtaaaa tagaggcacg atcggaagat ctggctgttc aattattaca ggggtatggt
     5161 ttaatagttt atgatttaaa ggcagtagag aaccaaggta tttttgataa actatttggc
     5221 aagaaaaaac atataggtac aaaagaattg tcattatttt tacgtcaatt ttctactttg
     5281 ttatcatcca aagtgccttt gatggattca ttaaaaactt tgttagccca aactaatagt
     5341 agtgctttaa aagatatgat ttttaattta atttcaggta ttgatgctgg attatcttta
     5401 tcgcaagcta tgtcaagaga gagtaatatt ttttcttctt tttatattga aatggtacgt
     5461 tcaggagaaa tttctggtag acttgaagag gtttttaatt atttagctga ttatgcggaa
     5521 aacgaagcta atttaaatac aaaagcaaaa tcagctatga tttatccaat atttattata
     5581 gtgatatttt tgcttgttgg tacaattata tctataagtg ttgcgccaca aatgatggat
     5641 atttttcaag aatttggtca aaacccacct ttattaacta gaatattgat tggttttggt
     5701 cagtttttaa tggattgggg tattattgta ggtgtgataa ttgtgggagt aataattgcg
     5761 atgataaatt attttaaaac taaagaagga caaaatgttt tgtcgttctg atctatgaag
     5821 atacccatta ttggtaatat ttataaagcg atttatatat ctcgttttgc tgaatctttt
     5881 ggtacattat tacatggtgg aatccctgta gtgcaggcat tggaagtagc tgggagcgca
     5941 acaggtaatt ttgtgtttga tgccatagga aaagaagtag ccgagggagt aagaagagga
     6001 gagcccatta gtaaattatt gcaaaattat ccagaatatt ttccaccatt agtttctcaa
     6061 atggtgtatg ttggagaaaa tactggaaga ttagatactt tattaaaacg tatttcagtt
     6121 tattatttta atgaagtaga aaacggattt aaagtattac ttgaaatgat tcagccagtt
     6181 ttgattgtgg tgattgctat ttttgtaggg attttaattg ctggtgtttt gttaccgatt
     6241 tatcaattag cagaggtggc atagttttat agaatatcaa aaatcagaat tataaaatgt
     6301 tcataaaaag taagtagtag aataaatact gataaccaaa taacaaattc ttgtaaataa
     6361 tttaattttc aattatggat ttaaataatc tttgtaaaga gattcgtaaa gaaattattg
     6421 agataactta tgaagcacaa tctggacata ctgctggccc cttgggcgct gtggagcaat
     6481 atgttagttt atatttttct ggtattttaa aagaaaatga taaaataatt atttccaatg
     6541 gtcattatgc tcctttattc tatataattt tggcccataa agggtattta gagaaatcta
     6601 aattaaaaac tttacgaaaa tttgggtcag cactacaagg gcatcctcat agaggtgata
     6661 ttcaaggtat agaaaatact tctggtccag taggtgaagg attgtcgcaa gctattggtg
     6721 tgtgtattgc tagcaattta gataaaaaca acaatcaaat ttattgttta atgggagatg
     6781 gggagcatga tgagggtaat gtttgagaag cagtaatgtt agcaggcaaa ttaaaattag
     6841 aaaatttaac tgccattatt gatagaaatt atattcagat tgatggtggt actgaggatg
     6901 ttatgccttt agaccctttg aaagcaaagt atgagagttt tggatgaagt gttttagaag
     6961 ttgatggaca taatattaaa caaattatta atgcgttaat aaaagctaaa aaaatttgag
     7021 acaaacctgt agtaattatt tgtcgtacta cgcctggtaa aggagtatct tttattgaac
     7081 ataattatgc ttggcatgga aaggtcgtta ataaagaaga aaaagaaagg gcattgcaag
     7141 agatcaatgc ttttaataat ttataatttt atatatattt attatgaatt tagaatcaaa
     7201 gaaaaacata tctgcttata gtgaatcagc aaaaaaacca acaaaagatc aaaaagattg
     7261 aaatactaat caaactataa acaaaaaaga tgttatagct atacaaaata ggttaattaa
     7321 aattgtcgaa gatttaaagc ataaaaataa aggtctatgg acacctcaat ttgaatctga
     7381 tacaaaaaat atagatgatt tgcaaacact tctgacattt tataattatt cgcctagatt
     7441 aacaagtcgg gccttgaaaa acgtaataga tgattcgact attagaaagt tgataaaaaa
     7501 acatcgggaa gagttttata atacttgaat tttgggttct gtggcacaga gaaaaaaaga
     7561 agcatttaca caacatgata gttatgatat aacgttaaga caaagaaatg aaaatgaggt
     7621 agcaactatt atggatatag aaaacgtatt agataaattt ttacgagcaa aacttgtggc
     7681 ggccaaaggt gataaaacaa aaatgagcaa aattgataaa ttgttggata gggtttctga
     7741 tgatgccata gaaattttta atacagccaa acatataaat aaacattatg ataagtctac
     7801 tatcaaagaa gctttttata aatgatgtgt taaacataaa atcgaatatc ccgataatag
     7861 taaacctcaa caagaacaaa ataaataatt cattatgttg aatttatctt taaaattaaa
     7921 ttcaaaaata tttgatcaaa atattataac cgaatctatg cgcgaaggat ttggacaagg
     7981 tttagtgata gcaggagaaa aaaataaaaa tgtaatggct ttgtgcgctg atttaactga
     8041 ttctttaaaa atgaatttat ttaaaaatcg ttttccaaag agatttattg aagtaggtat
     8101 tgctgaacaa aatttagcaa ctgttgcatc tggattagcg tcttgcggga aaattccttt
     8161 tattggatct tttgctgtgt tttcgcctgg tagaaattga gaacaaataa ggacaagcat
     8221 ttgttataat aatgtaccag taaaaattgt tggttcgcat gctggattaa atgttggtcc
     8281 tgatggggca acgcaccagg cattagaaga cattgcgtta atgcgtgttt tgcctaatat
     8341 gattgtagaa gtgccatgtg attattttga agcaataaaa gctactacct taattgcaga
     8401 aaatggaaaa ccgagttatt taagattaga aagagaacct tctgtggcta ttacgtcatt
     8461 attgtctcct tttgaaatag gtgttgcgta tgctttgtga ataggagatg ttccggatgt
     8521 tttaattatt ggctgtggtt ctggtttata tgaagcgtta ttggcagcta aagaattgaa
     8581 aaaagagcaa atagaagtag tagttttgaa taatcataca attaaaccaa tggatgaaaa
     8641 aacaattttg caatgagcaa aaaaatgtat tggagtggta actattgaag atcatcaaat
     8701 ttatggaggt atgggtagtg cagtagcaga attattagcc aaaaaatgtc ctaaaaaaat
     8761 agaaatgtta ggtgtcaatg atagttttgg tgagtctggg acaaaacaag aattatatac
     8821 aaaacatcat ttaacatccc aagatataat attggcagtt agagacattg tgcgttgaaa
     8881 aaagaaataa aatatgttat acttattata aatatgaatt aatgattaaa ggtttagaag
     8941 taaaaacaaa ttagcaaata attgaatttt agaatatgga taaacatcat aaatcttata
     9001 attatttata tattgcctta gcaggatgta tactaattgg tatattatgt attagttata
     9061 ttaaagcttg gaatattcct actcaaaatc caccagctgg taatgtnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnngtac tggcaatatt gtttcatctt ctggtgatgt
     9241 taagggtgct cgtctttgta ttggtgataa ttgtcaagat tcatggactt ctggaggatc
     9301 tgtaccaact ggaggtatga tttttagtga taatcaaaac aaccctaatt tattgagcgc
     9361 aggttatttg aatacgggta aattgattaa ttatgttgag tgtgttaatg gacaatttaa
     9421 tccaaatgga gaatgtattt gtgatatttg ttgttgtcaa cattatagtg aatttttata
     9481 tgtttatcaa aaattacctt aaattattat aatgaattgt gattctatag ttataggtac
     9541 agcaactttt gatactttct taaagggttt taaatatgaa attataaata gtaatgttta
     9601 tgcaaatggt aaggcattgg ctattgctta tggcagtaaa gaaaaagtag aaattataga
     9661 tcatcaagca gggggtggag ctgtaaatat agctttgtgt ttagcaagat taagattaaa
     9721 aactaatatt ataattagaa tttgtaaaga ttatgaggga gattttattt tgaatacatt
     9781 gaaatcccaa ggaaatattt ttactaatta tatacagttt gatagaaata taaaaacggc
     9841 ttcctcgtgc gtattagtta cgggcgatgg cgagaggact attttatcat ataaggggtg
     9901 tggtcaaaat ttagttatag atagtaagat attgaataaa ataaaaaccc atatgttagt
     9961 agctagtact attgctggta attctacaaa ttttcaagcg attttcgaat ataaaaaaca
    10021 acatcccgaa actatactta ttgctaatcc cggagtggcg gatatagaaa ttttgagaaa
    10081 acacaaagag tgattacatt attatgatgt ttttattgta aatcaagaag aagctactta
    10141 tttatcagat attgattata agaaagtaaa agagttgttt acttgattgg ataaatatgt
    10201 tgaaggaatt tgtattatga caatgggagc tgacggttta atattttctg atagaaataa
    10261 tatttataca gctaaaaatt ctgaagatgt tgtagttgtt gatagcactg gtgcaggaga
    10321 tgcttttgta agtggattta cggctggtta ttatcaaaaa cacgacccaa tatatgctgt
    10381 aaaatttggc ttagctaatt cttcgcaagt aattgaaaaa ataggtgcca atataggatc
    10441 tttgagtctt gctgatttaa aattagaaag atgaaaaaat gattttgaaa ttattcaagt
    10501 caataaaatt taataaatat tgaaaattaa aatatggaaa gatatattca gccaatttta
    10561 aaaaataata aatgttttat tcttgcctat gatcatggtt ttgtacacgg aggaagtgat
    10621 tttaataata aaaattgtga tataaatttt atttttgatt tggctttaca gggtggtttt
    10681 acaggcatag ttttgcataa aggattggca gagtatttta gagattcaaa atattttaat
    10741 aaagtgcctc ttattgttaa attaaattca agcacttctt tgccaataaa tacacaaata
    10801 gatacggcga aattatgttc ggttatatat gctaaaaaat taggcgcagt agcagttgga
    10861 tatacaattt atttaggatc agaaaatgaa aataagatgt ttgaagaatt ttcagatatt
    10921 caagaacagg cacatgcctt aaatatggga gcaatagctt gaatttatcc taaagaaaag
    10981 aaaaatatat tagaaacaaa tcaaaaaaat gattttataa ctcaatatgc cacaagaata
    11041 ggtttggaat tgggtgcaga tatgataaaa attaaaaata atgcaacaaa agatggtttg
    11101 aagcgagcaa tagaattagc taagcccgta agggtagctt tggcaggagg tgaaataata
    11161 aatagtcaac aatttttaga tataacgaaa caatttatgg atattggtgg gtgtggttta
    11221 gtagttggcc gaaatatttg acaacgtgat gatgccttag aattttctca aaaaatcaaa
    11281 cagataattt ttgagtaaac aaattattat gcccagatct gtattatgat ttagaataat
    11341 agtccttggt tttatactgg gacttttttt gtttcaaaaa cacataaata tgtcaaaaat
    11401 tagtgaatat tgggttggaa gagaagtcca aattgagggt attgttatgc gtaatccagt
    11461 agaaatagaa gataaaaaat atttagcagt aaaaccaatt t
//
LOCUS       DAOTJA010000018        11226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6156_length_11226_cov_5.417152, whole genome shotgun sequence.
ACCESSION   DAOTJA010000018 DAOTJA010000000
VERSION     DAOTJA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_11226_cov_5.417152"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..258
                     /locus_tag="P5241_01920"
     tRNA            187..258
                     /locus_tag="P5241_01920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Thr,seq:ggt)
     gene            424..582
                     /gene="rpmG"
                     /locus_tag="P5241_01925"
     CDS             424..582
                     /gene="rpmG"
                     /locus_tag="P5241_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRV32304.1"
                     /translation="MKKENLTKLQCSVCKHINYYTRKNKKTVQRKIELKKHCPYCRKH
                     TLHKETKK"
     gene            810..1175
                     /locus_tag="P5241_01930"
     CDS             810..1175
                     /locus_tag="P5241_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32305.1"
                     /translation="MKFKETMPTLIENSLAVDYKYLKDALIKGSMSFKQVRDKLLEFD
                     QSDLSRKAADKNLEFLLDPIIIDFVNKNTKILEGYYRFLSLTEFHVAQRIASNDIQEA
                     KKHFNNALNSAKRGFSTKS"
     gene            1197..1340
                     /locus_tag="P5241_01935"
     CDS             1197..1340
                     /locus_tag="P5241_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32306.1"
                     /translation="MLYIQSKKIPDSIIEQVEEEKNVKILQNFNKGLAERGFPEYNKD
                     YGR"
     gene            1632..1889
                     /locus_tag="P5241_01940"
     CDS             1632..1889
                     /locus_tag="P5241_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32307.1"
                     /translation="MHKVNKKGTLNTSFGIAWDFDGTIIKSQKEVHAPIESKILKDLG
                     IDINDEEICSRYSGVRTQDYFLKLLNDYNIKVNVDQLIEKK"
     gene            1896..2114
                     /locus_tag="P5241_01945"
     CDS             1896..2114
                     /locus_tag="P5241_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRV32308.1"
                     /translation="MLQEARTRDWEFLPGVIQFIIQAFHYGIKQSIGTASHKDYVDII
                     LERYELKQYISYIVSGDDVIKGKPDPET"
     gene            2175..2312
                     /locus_tag="P5241_01950"
     CDS             2175..2312
                     /locus_tag="P5241_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32309.1"
                     /translation="MAGLKAAQKAGMHSIYIGANPLYQADICIKSFDDLDIYKLAKLC
                     N"
     gene            2373..2443
                     /locus_tag="P5241_01955"
     tRNA            2373..2443
                     /locus_tag="P5241_01955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2405..2407,aa:Trp,seq:cca)
     gene            2774..3355
                     /locus_tag="P5241_01960"
     CDS             2774..3355
                     /locus_tag="P5241_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRV32310.1"
                     /translation="MKFDNKHPDAVLVFGGDGTILEASRKYKDKKSIIFGFNLGQVGF
                     LASVRDKKFFYYGLNNFLNQKYHICKRYFLKIELIRNKKIINSAFALNDTVIQSPTNM
                     VKLNVSSNKHTFQLIYGTGVLISTATGSTAYNLSAHGPIVSPDIKCLIITELFDHNLP
                     TPSIILNHHQEISIKVIDYRLQDVVLISDGNNF"
     gene            complement(3685..3963)
                     /locus_tag="P5241_01965"
     CDS             complement(3685..3963)
                     /locus_tag="P5241_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32311.1"
                     /translation="MFLECPDLTIFLISSLPAETADNSNNSAFKLLAYILAKVVLPHP
                     GGPHKIKENRMPCSIAFRRGLFLPTKCSWPTNSSKFLGLIFEANGSIY"
     gene            3860..4204
                     /locus_tag="P5241_01970"
     CDS             3860..4204
                     /locus_tag="P5241_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV32312.1"
                     /translation="MYAKSLNAELFELSAVSAGKEDIRKIVKSGHSRNKILFLDEIHR
                     FNKAQQDFLLPYVETGELTLIGATTENPSFEIISPLLSRCRVFVLNELSEENIKEIIK
                     RTKIKMDKKSID"
     gene            4214..4810
                     /locus_tag="P5241_01975"
     CDS             4214..4810
                     /locus_tag="P5241_01975"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32313.1"
                     /translation="MANGDARQAITVLENCLSLYKEINVENLKNTLQSKFLRYDKKAD
                     EHYNLISAYIKSLRASQVDAALYYLARLIESGEDPKFIARRMVIFASEDIGVAVPTAL
                     VVANEVFRAVETIGLPEAQINLAHGTVYLANCKKNRSAYNAYFEALKDVKTYGNLPIP
                     LKIRNPETKLMEKLDYGKGYQQYDKDSYLPDKIKNKKY"
     gene            4927..5328
                     /locus_tag="P5241_01980"
     CDS             4927..5328
                     /locus_tag="P5241_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HRV32314.1"
                     /translation="MEENIPLEELERYFDQNQSWGLASGIDLHGCNSDYVRSESKIKE
                     FVVALCDLIKMKRFGDCVVVDFGEDPRVSGFSMTQLIETSLISGHFVNQNNDIHLDIF
                     SCKYYDPKIAAEFAANFFQATDYTYYYKIRK"
     gene            5398..6168
                     /locus_tag="P5241_01985"
     CDS             5398..6168
                     /locus_tag="P5241_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32315.1"
                     /translation="MIKTLLKQYINNAVHKITKNDVSFDIRIPSKEFGDYSSNIGLVL
                     CKQLHQSPQDINLRLIDILNKSKDFTKYFSQVQEANGFLNFTLNPKFVHQEIKNINKQ
                     KEKYFMVKNSTKKKPKVQVEFVSANPTGPLTLANARAAFFGKALADILSYNGFQVEKE
                     FYVNNTGKQIYNLGIAIIKAWKLNPGDYGYKFNELDIYKGNYINEISVIIRSKIKGDL
                     ETYVKKHSPDDIGSMAVKENLAQIKYVLSKKANIVFDN"
     gene            6466..7080
                     /locus_tag="P5241_01990"
     CDS             6466..7080
                     /locus_tag="P5241_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HRV32316.1"
                     /translation="MKETGIIKPSSRFDIILIQFMTLLKNGKPFKMSKRQGNFITFED
                     FIDAVGKDAASFFILERSTDTHLDFDYDLAIEQSDKNPVYYIQYAYARICSIQNKFTK
                     SVKTHKQNIDLSLLNNKNEIYLMKEILRLPDILHDISLTFEVHLLTNYVVNLAKAFHS
                     FYQNCPIIKSEEKLQASRLFLLDATKITFELCFSIMNIDTKKHM"
     gene            7085..7546
                     /locus_tag="P5241_01995"
     CDS             7085..7546
                     /locus_tag="P5241_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32317.1"
                     /translation="MNNQNQNDILMIIDSNALVHRAYHALPSLTSPSGVLTNAAFGYF
                     LILFKAIEEIKPKYIVATFDVAAKTFRHEMFDKYKATRIKSPDNLYAQIPIVKEILQN
                     MNIKILEAPGYEADDIIGTVVQKMKHNKNLDIIIVTGDLDTLQLIDNNVKV"
     gene            7577..8308
                     /locus_tag="P5241_02000"
     CDS             7577..8308
                     /locus_tag="P5241_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRV32318.1"
                     /translation="MYDYETFRKKYGMEPFQFVDYKGLKGDPSDNIPGIEGIGDKTAK
                     ELISKHHTIEELLKDAKDIVDDPLVSNKDKKIYKKILEYKDEALFSKQLASISLDVPI
                     DFTLDDMRYQISNNSGLQETLKKYGFYSLIRKIDIKNTPKTSYSVKNIFDYQQITISN
                     IVEANKYLASATCIYLMLDPDNNLYFSFVNNENKTIVFSINNTKIKDLQLPKAVFVTF
                     DAKQLYKTYPNIFGDIFFEDLKIMS"
     gene            8531..9709
                     /locus_tag="P5241_02005"
     CDS             8531..9709
                     /locus_tag="P5241_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRV32319.1"
                     /translation="MEENGIGLDKKYLETLLDDLKPQIQKLEKEIYKLAGQEFNINSP
                     KQLTDILFNVLKINTKGLAKTSVSKAVSTNSIELEKIKNEHPIIDLILKYRELNKLVT
                     AFILPLPSFISSKDQKIHAIFQQTGTATGRLSSDSPNLQNIPTRKDYGTLVRKAFIPS
                     PGYLFVSFDYSQMELRIAAFLSQDNAMLKSFKEGKDFHTYTASLIFDVPYDKVTKDQR
                     SKVKALNFGIVYGMGPKAFAQTAGISKEEATKYIEDYFIHYYKLKDYIDTLKDNAKAK
                     GYCETLFGRKRFLPFIGVQSREGADQERIAINMPFQGLAADLIKISMVKIFDYLKENQ
                     LLDFVKLNVQIHDELLFEIKSDIINEIIPTIQRFLEDNELGVPLKVNISIGNNWGDLK
                     "
     gene            9913..11070
                     /locus_tag="P5241_02010"
     CDS             9913..11070
                     /locus_tag="P5241_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV32320.1"
                     /translation="MDFLQRVARIEIEKSQVNGILESMSDGVISYTQNFQIIAVNESL
                     EYICNIKKQDVLGKIISPEWDNNPKFSLLAKIVFPTLAPTIYRLSDSDYPSRVVLHLE
                     TPEEKILEVTTNRIFNKFTNSYIFIKIIKDRTREEQLLKLKSDFISIAAHQLRTPITG
                     ISWSFDMLKDESVGTLTDKQKEIIALANDAINEMKSTIEDLLEAAKIEEGKSEYNFQL
                     LQLEPIINEVISMYKPKCQEKNIKIIFNKPANPIPLLSLDKEKIKIAIQNIIDNGIKY
                     NVQNGEVRINIQKLVDKPYIQVDIEDTGIGIPAKDLPNMFNKFFRSDNVIKQDTRGTG
                     LGLHVTKNIIENHGGKIWIESIEKRGTTIHFVLPTDPALIPPQYQKPLNIL"
BASE COUNT         4262 a         1404 c         1717 g         3843 t
ORIGIN      
        1 atttacgggc gtgtagctca gtgggctgaa actcaaagta ttgagtttca gcgggtaaac
       61 ttcaaaatac gtgtttgatt tatcaaacac aatagaaata cgtagtttac cccggaagag
      121 cgttcacgaa gtgttgatcg ctggatttaa taaataatat ataaaagtaa tattaatata
      181 aatattgcca gagtggctca gtggtagagc acgtcattgg taatgacgcg gtcgtgggtc
      241 cgattcccac ctctggctca gaaaaattgt gcttttgtcc aattttttag tacttagtaa
      301 tttgtactta gtagttagag aaaaaaggag aatatttttt agatattaag cataattttt
      361 ttctctaaat acaaaatgct tacactaaat acgaattaat taaaagtaat ttaaatacat
      421 accatgaaaa aagaaaattt aaccaaatta caatgtagcg tttgtaaaca tattaattat
      481 tatacaagaa aaaacaaaaa gacagttcag agaaaaattg agcttaaaaa acattgccca
      541 tattgtcgta aacatacact gcacaaagag acaaaaaaat aaattaaaaa ttaaacatct
      601 atttttattt tacgataatt gttggttatt attagtgcta taaaggggtg tagctcaatt
      661 ggcagagcaa cggtcttcaa aaccgaaggt tgtaggttcg actcctatca cccctgcaaa
      721 cccttgacaa aagtcaaaaa gatttttgtt tgcaaaaata ttgaaaaagt ggtagaataa
      781 taataattaa aatattaaat taatccttca tgaaatttaa agaaacaatg ccaactctta
      841 ttgaaaatag cttagctgtg gactataaat atcttaaaga tgcattaata aaagggtcta
      901 tgtcttttaa acaagtcaga gataaacttc ttgaatttga tcaatctgat ttgtcaagaa
      961 aagcagccga taaaaatctt gaatttttgt tagatccaat aattattgat tttgtaaata
     1021 aaaatacaaa aattctagaa ggatattatc gttttctaag tttgactgaa tttcatgttg
     1081 cacaaagaat tgcctctaac gatattcaag aagctaaaaa acattttaat aacgcattga
     1141 atagtgccaa gcgtggattt tctacaaaaa gttgaaaagc atatattgag ggtaccttgt
     1201 tatatatcca atctaaaaaa atcccagata gcataattga acaagtagaa gaggaaaaaa
     1261 acgtcaaaat tttacaaaat tttaataaag gattggccga aaggggattt cctgaatata
     1321 ataaagatta tgggagataa attatggtta taaggtttca attttttagc catctttttt
     1381 gttataatct tacgatattc actgtatttc tcataaagaa agttctaact tcgtctagtt
     1441 gcccctgcat taggtagatt gttcttatcc ccaattttta gtacttagta atttgtactt
     1501 agtagttaga aaataaaatt gctaataaat agtatgttgt ataataagtg tgatactttg
     1561 ttcgaatcag tataaaagct tagcagtaaa ataatttatt attttgagat ttgaaaatta
     1621 aataatattt tatgcataaa gttaataaaa aaggcacatt aaatacaagt tttggcatag
     1681 cttgggattt tgatggtact attattaaga gtcaaaaaga agtacatgcg ccaattgaat
     1741 ctaaaatttt aaaagattta ggtattgata taaatgatga agaaatatgc tcgagatatt
     1801 ctggtgttag aacacaagat tattttttaa aattattaaa tgattataat attaaagtta
     1861 atgtagatca attaatagag aaaaagtgaa aattaatgct acaagaagcg cgaaccaggg
     1921 attgggaatt tttacccgga gttattcagt ttataattca agcattccat tatggcatca
     1981 aacagagtat tggtacagct tctcacaaag attatgttga tattatttta gaaagatatg
     2041 aactaaaaca atacatttca tatattgtaa gtggtgatga tgttatcaag ggaaaaccag
     2101 atccagaaac atgattaaaa tgtgcagaat tactaaaatt acctcctaat aaaattattg
     2161 ttattgaaga tggaatggct ggtctaaaag ctgctcaaaa agcaggtatg catagtatat
     2221 atattggggc caatccgtta taccaagctg atatttgtat taagagtttt gatgatttgg
     2281 atatttataa acttgcaaaa ttatgtaatt aagtggttgt tgttggaact tgcaaagaag
     2341 atgtgttgct gtattaattt ttgatatttt ttgggtgtgt cggttaatgg taaaccaacg
     2401 gtctccaaaa ccgtgactgg gggttcaaat ccctccacac ccgcaattaa catagctttt
     2461 aactattggt tattagtaat taactaaaaa tcacaaatca acattgacac attaataatt
     2521 taaactactc attgaacata aatagtatta cttttgtttt acttcgatat caaaattatg
     2581 tttattttta tgtgtcagag actaactact tatgattttt cttttattac aaaatatgct
     2641 gtaatataaa tatatttaat ttatagctat gaaaaaaata aaatcagtca caatatattt
     2701 tcgtcaaaat caaaaacaag ctgttctatg atctcaaaaa atagaacaat atatcaagca
     2761 acaatacaaa acaattaaat ttgataataa acatcctgat gctgttttag tttttggtgg
     2821 agacggcact attcttgaag ccagtagaaa atataaagac aaaaaatcta ttatttttgg
     2881 ttttaatttg ggtcaagtag ggtttttggc gagtgttaga gataaaaaat ttttctatta
     2941 tggacttaat aattttttga atcagaaata tcatatctgt aaaagatatt ttcttaaaat
     3001 tgagttgatt cgtaataaaa aaataataaa cagtgctttt gcacttaatg atacggttat
     3061 ccaaagccca accaatatgg taaaacttaa tgtttcttct aacaaacata catttcaatt
     3121 gatttatggt acaggagttt taatttctac agccactggt tctacagctt acaatctatc
     3181 agctcatggt cctattgtta gtccagatat taaatgttta attatcactg aattatttga
     3241 tcataatttg cccactccta gtattattct taatcatcat caagaaatta gtatcaaagt
     3301 aattgattat cgtttacaag atgttgtttt aatttctgat ggtaataatt tttgaaatct
     3361 tcaaaaaaat gatattatta aaatcaaatt atctacaaaa tctattgatt ttgcggagat
     3421 tgacgataat tactttttaa aaagtttaca cgaaaaattt ttatttcact aattatgtat
     3481 attgtattta ttttaggtac aaggtcagaa actcttaaag ttgggggagc tataatgtta
     3541 aaatctatat ctaaaaatga aattattaaa gtttacaaaa actgatcaat aaaaaagtaa
     3601 attgatataa tccttttggc aatggtaagg ccacgatgaa gatatttaaa attctaatca
     3661 aataattaaa cattatattt aaaattagta aatggaacca ttagcttcaa aaataaggcc
     3721 taaaaattta gacgaatttg ttggccaaga acatttagtt ggtaaaaata aacccctgcg
     3781 gaatgctatt gaacaaggca ttctattttc ttttatttta tgaggccctc cgggatgcgg
     3841 taaaactact ttagccaaaa tatatgccaa aagtttaaat gcagaattat ttgaattatc
     3901 agctgtttct gctggcaaag aagatataag aaaaattgtc aaatctggac attcgagaaa
     3961 caaaatatta tttttggacg aaattcatag attcaacaaa gctcaacagg attttttatt
     4021 accttatgta gaaactgggg aattaacttt aattggtgcc accaccgaga atccgagttt
     4081 tgaaattata tcaccattgc tttcaagatg tcgtgttttt gtattgaacg aattatcaga
     4141 agaaaatatt aaagaaatta tcaaaagaac aaaaattaag atggataaaa agtctattga
     4201 ttgattggtt tcaatggcca atggtgatgc tagacaagct attacggttt tagaaaattg
     4261 tttatcttta tacaaagaaa taaatgttga aaatctaaaa aacactttac aatcaaaatt
     4321 tttaagatat gataaaaaag ctgatgaaca ttataatttg attagcgctt atataaaaag
     4381 tttaagagca agccaagttg atgctgcttt atattattta gctcggttga ttgaaagcgg
     4441 ggaagatccc aagtttattg ctcgtcgtat ggtaattttt gcttctgagg atattggcgt
     4501 agctgtacca acagctctgg tagttgctaa tgaagtgttt agagcagtgg agactatagg
     4561 tctacctgaa gcacaaatta atttagcaca cggaactgtt tatttagcta attgtaaaaa
     4621 aaatagaagt gcttataatg cttattttga agcactaaaa gatgtaaaaa catatggcaa
     4681 tttgcctata ccgcttaaaa tacgtaatcc tgaaacaaaa cttatggaaa aattagatta
     4741 cggaaaaggt tatcaacaat atgacaaaga ttcatactta cctgataaaa ttaaaaataa
     4801 aaaatattga agcatagata aataatagat attatttata ataaaattgt attgggtatt
     4861 gcatttttat gtaaaatatg atatgctctg cgtgttttaa ttaataaaag tttaaacaaa
     4921 taaccgatgg aagaaaatat accgcttgaa gaactcgagc gatactttga tcaaaatcaa
     4981 agttggggat tggcatcagg cattgattta cacgggtgta attctgatta tgttcgtagt
     5041 gaatcaaaaa ttaaagaatt cgttgttgct ttgtgtgatt taataaaaat gaagaggttt
     5101 ggagattgtg ttgtggtaga ttttggagaa gatccaagag tatctggatt ttcaatgaca
     5161 caacttatag aaacgtcatt gatttcagga cattttgtaa accaaaacaa tgatatccat
     5221 ttagatattt tctcttgtaa atattatgac cccaaaattg ccgctgaatt tgctgctaat
     5281 tttttccaag ctactgatta tacttattat tataagatca gaaaatagca taattaaaaa
     5341 cattgaattt tttttataat tgtgatacac ttagttatag taactaagtg tatttttatg
     5401 ataaaaacac tattaaaaca atatattaac aacgctgttc ataagataac taaaaatgat
     5461 gtaagttttg atattaggat accttcaaaa gaattcgggg attattcttc taatatagga
     5521 ttagttttat gtaaacaatt acatcaaagc ccccaagata tcaatttacg actgattgat
     5581 attttaaata aatccaaaga ttttacaaaa tatttttctc aagtacaaga ggcaaatggt
     5641 tttttaaatt ttactttaaa tcctaaattt gtacaccaag aaataaaaaa tataaataaa
     5701 caaaaagaga aatactttat ggtaaaaaat tctaccaaaa agaaaccaaa agtacaggta
     5761 gaatttgttt cagccaatcc tactggtcct ttaacgttag ccaatgcccg ggctgcattt
     5821 tttggtaaag ctttagctga tattttgtct tataatgggt ttcaagtaga aaaagaattt
     5881 tatgttaata atactggtaa gcagatatat aatttaggta ttgccattat taaagcttgg
     5941 aaactaaatc ccggagatta tggttataaa tttaatgaac tagatatcta taaaggcaat
     6001 tatattaatg aaatttctgt tattattcgt tctaaaatta aaggtgattt agaaacatat
     6061 gtcaaaaaac atagtcctga tgatataggt tctatggctg tcaaagaaaa tttagcgcaa
     6121 ataaaatatg tgctatccaa aaaagccaat atcgtttttg ataattgatt tttagaaact
     6181 gatcttaaaa aattaggtta tcttgataaa gtttttaaat ggttagtaaa cgaaggcttt
     6241 acttataaaa aagatggagc cttgtggtta aaaaccacca agatgtttaa tgatgataaa
     6301 gatagggtat tgcaacgaag tgaaggtggg ttagaaactt attttttaaa cgatattgct
     6361 taccatttag aaaaatttag ccatagaaaa tttgattgag taattgatat ttttggtgcc
     6421 gatcatttta ctcatgctca aaaaatgatt tgagttgtca acaagatgaa agaaaccggg
     6481 attattaaac cctcctctcg gtttgatatt attttaattc agtttatgac cttacttaaa
     6541 aatggtaagc cttttaaaat gtcaaaacgt cagggtaatt ttattacttt tgaagatttt
     6601 atagacgctg taggcaaaga cgctgcttct ttttttattt tagaacgaag tacagatacc
     6661 catttagatt ttgattatga tttagcaata gaacaatcag ataaaaaccc agtttattat
     6721 atccaatatg catacgctcg tatttgtagc atacaaaata aatttacaaa atctgttaag
     6781 acccataagc aaaatattga tttatcactt ttaaataata aaaatgaaat ttatttgatg
     6841 aaagaaattt tgcgtttgcc agatattctt catgatattt ctttaacttt tgaagttcat
     6901 ttgttaacta attatgtagt aaaccttgcc aaagcttttc attcgtttta tcaaaattgt
     6961 cctattatta aatcagagga aaaattacag gcatctcgtt tgtttttatt agatgccacc
     7021 aaaattacat ttgagttgtg ttttagtata atgaatattg atacaaaaaa acatatgtaa
     7081 taatatgaac aatcaaaatc aaaatgacat tttaatgata attgatagca atgctttagt
     7141 tcatagagcg tatcatgcat tgccaagttt aacatctcct tcgggtgtgc taaccaatgc
     7201 cgcttttgga tattttttaa tattattcaa ggctattgag gaaataaaac ctaaatatat
     7261 tgttgccact tttgatgttg ctgccaaaac ttttagacac gaaatgttcg ataaatataa
     7321 agcgacacgc ataaaatccc cagataattt atatgctcag attcctattg taaaagaaat
     7381 tttacaaaat atgaatatta aaattttaga agcgcctggt tatgaagccg atgatattat
     7441 cggcacagta gtacaaaaaa tgaaacataa taaaaattta gatattatta ttgttacagg
     7501 tgatttagat actttgcagt taatagataa taatgtaaaa gtatgaactc tcaaaacagg
     7561 tatttcgcag agctttttgt atgattatga aacttttaga aaaaaatatg gtatggaacc
     7621 atttcagttt gttgattata aaggattaaa aggcgatcct tcagataata ttcctggcat
     7681 tgaaggtata ggggacaaaa ctgccaaaga attgattagt aagcatcata cgatcgaaga
     7741 attacttaaa gatgccaaag atattgttga tgatccctta gtgtcaaata aagataaaaa
     7801 aatttataaa aaaatattag aatataaaga cgaagcttta ttttcaaaac agcttgcttc
     7861 tattagttta gatgttccaa tagattttac tttggatgat atgcgttatc aaatcagcaa
     7921 caacagtggt ttgcaggaaa cattaaaaaa atatggtttt tattctttga ttagaaaaat
     7981 agatataaaa aatactccca aaacttcgta ttctgttaaa aacatttttg attatcagca
     8041 gattacaata tccaatattg tagaagctaa taaatatctt gctagcgcta catgtattta
     8101 tcttatgtta gatcctgata acaatttgta tttttctttt gtaaacaatg agaataaaac
     8161 tattgttttt agcattaata atactaagat caaagatcta caattgccca aagctgtttt
     8221 tgtgactttt gacgctaaac aattgtataa aacatatcca aacatttttg gcgatatttt
     8281 ttttgaagat ttaaaaatta tgtcttgact tatagatcca gaaaaaaaag attattcttt
     8341 tacagcaatt gccaaacaat ttataaaaga aaagcaatca aattatcatt tttttcaaaa
     8401 ttttgaaata agtgatttaa tagccttaat gcctgaatta tatcaaaaaa tcctttttaa
     8461 cataaataat aataaattag atattgtttg aaataaaatt gaaaaacctt taattagggt
     8521 gttggctaat atggaagaaa atggtatagg attagataaa aaatatttag aaacattatt
     8581 ggatgattta aaaccccaaa ttcaaaaatt agaaaaagaa atatataaat tagcaggaca
     8641 agaatttaat atcaattcac ctaaacagct tacagatatt ttatttaatg ttcttaaaat
     8701 taataccaaa ggacttgcta agaccagtgt tagtaaagca gtgtcaacta atagcataga
     8761 attagaaaaa attaaaaatg agcatcctat aatagatcta attttaaagt atagagaatt
     8821 aaataaatta gttacggcat ttattcttcc attgccgagt tttattagtt ctaaagatca
     8881 aaaaattcat gctatttttc aacaaacagg tacagccaca gggcgtttgt cgtctgattc
     8941 gccaaattta caaaatattc caacgagaaa agattatgga acattagtac gtaaggcttt
     9001 tattccgagc ccaggctatt tatttgtttc ttttgattat tcccaaatgg agctacgcat
     9061 tgctgctttt ttgtcgcaag ataatgcaat gcttaaaagt tttaaggagg gtaaagattt
     9121 tcatacatat actgcgagtt taatatttga tgttccttat gataaagtta ccaaagatca
     9181 acgttctaaa gtaaaagctt taaattttgg tattgtttat ggtatgggac ccaaagcttt
     9241 tgcccaaaca gctggtatta gcaaagaaga agctacgaaa tatattgaag attattttat
     9301 tcattattac aaactaaaag attatattga tactttaaaa gacaatgcca aagctaaagg
     9361 atattgtgaa actctttttg gacgcaaaag atttctgccc tttataggag ttcagtctcg
     9421 agaaggagca gatcaagaaa gaattgctat aaatatgccc tttcaaggcc ttgccgctga
     9481 tttaatcaaa atatctatgg taaagatttt tgattatttg aaagaaaacc aattattaga
     9541 ttttgttaag ctaaatgttc aaattcatga cgagttgctt tttgaaataa aatctgatat
     9601 aattaatgaa attataccta ctattcaaag gtttctagaa gacaatgagt tgggtgtccc
     9661 attgaaagtt aatataagta ttggtaataa ttggggagac cttaaataaa ataatatcat
     9721 tgtaaaatat aacgaatgaa aacattgttc caaaatatta aaaattgaag agctattgtt
     9781 gctattatta atatatcttt gatattaact attagtatat taaatattat aaatattgct
     9841 atacccaata tattagccta tattatttat gttatagtta ttattttatc ctgtattttg
     9901 tttattgttt ttatggattt tttacaaaga gttgctcgta tagaaattga aaaaagccaa
     9961 gtcaatggta ttttggaaag tatgagtgat ggtgttattt cgtatactca aaattttcaa
    10021 attattgctg taaatgagtc tttggaatat atttgtaata ttaaaaaaca ggatgtatta
    10081 ggtaaaatta ttagtccaga gtgggataat aatcctaaat tttccctttt agccaaaata
    10141 gtttttccta cccttgctcc tactatttat cgtttaagtg attcagatta tcctagtaga
    10201 gtagttctac atttagaaac tccagaagaa aagatattag aagttactac caatcgtatt
    10261 tttaataaat ttactaattc ttatattttt attaaaatta tcaaagatag aactcgtgaa
    10321 gaacagttat taaaattaaa atctgatttt atttctattg ccgctcatca attacgtact
    10381 cctattactg gtatttcttg gagttttgat atgcttaaag atgaaagtgt ggggacactt
    10441 acagacaaac aaaaagaaat tattgctttg gcaaatgatg ctataaacga aatgaaatcc
    10501 actattgaag atttattaga agcagctaag atagaagaag gtaaatctga atataatttt
    10561 cagcttttgc aactcgaacc tataattaat gaagtcattt ctatgtataa acctaaatgt
    10621 caagaaaaaa atattaaaat tatttttaat aaaccagcaa atccaatacc attattgtct
    10681 ttggataaag aaaaaataaa aatagctata caaaatataa ttgataatgg tattaaatat
    10741 aatgttcaaa atggagaagt gagaataaat atacaaaaat tagttgataa accatatatc
    10801 caagtagata ttgaagatac tggcatagga atccctgcta aagatttgcc taatatgttt
    10861 aataaatttt ttagatcaga taatgttatt aaacaagata cgaggggcac agggctgggg
    10921 ctacatgtca ctaaaaacat tattgaaaat catggtggaa aaatttggat agaatctatt
    10981 gaaaaaagag gtactactat tcattttgta ttgccaacag atcctgcttt aatacctcct
    11041 caataccaaa agccattaaa tatattataa tatataaatt tattaacaaa gaggccgatt
    11101 cattttttat aattctatag taaacagata tacaaaaaca atatatacaa tttaacaaat
    11161 attttaatcc tatactatgt ttaattattc taatcattca aaaaagtatt atgtatatat
    11221 atttat
//
LOCUS       DAOTJA010000019        10381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6739_length_10381_cov_5.142553, whole genome shotgun sequence.
ACCESSION   DAOTJA010000019 DAOTJA010000000
VERSION     DAOTJA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_10381_cov_5.142553"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="P5241_02015"
     CDS             <1..1361
                     /locus_tag="P5241_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HRV32321.1"
                     /translation="IGVGSDAKKFVLQSVGRGVRIEPLKGKRKRLLNLFNAQQIEPQL
                     FEQIKNFVLPIESLFIFGTNAENLKEIITTLKTEKQDKNLGDAFILNSEAQNHLLLVP
                     VYKTTDRIFAEEKEIQKYPVSREDFDLTAQFYRFIGDKVAVAKYNCDVKVLKKTKESF
                     EKKERYYDFGDYDFGEKNSLFEPLFEPELILDRIFDYLGVKSKEFDKFKELENEIVHF
                     KKIRFSGGEKYEEIKRKIEEIRQKPEREKELKKIFKKGNQKEYDRQRSLFEQAENFEI
                     NNQKIKIKYLANHYYLPVIVSETEKIDYLNHIINVNSEVQFIEQLEEYLTQPNNVFTQ
                     FDWWMFSKIDQTIDEVFIPYYNHKKNGMDNYHPDFIFWLQKGNDYLILFVDPHGTTYS
                     DINKKIDYFSKIFEVRETKENKIFPSNQFGITEKFNIRVKLLLKPAQGGLVAVGENYQ
                     QY"
     gene            1464..2189
                     /locus_tag="P5241_02020"
     CDS             1464..2189
                     /locus_tag="P5241_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32322.1"
                     /translation="MFNLTFSYNLNKDLDNYLNAIYKFRYLKHGRKNIEKTVSKYLSQ
                     QELTKIKTSKNQKAATLEIKKILLKWLAKNQDLINLNQKSLTESWNNRQDKFVTFSEE
                     FFEKKFNLLPATAYFTTLPICPYYYPDWFMISIKEGLEGQLHTIYHELFHFMFIKHYG
                     NYCNNKHLTQLEFESIKEALTVFLMTPPFNKINTLYEQGYPQEQELRQFMLKTYQQNH
                     KFISLLNNSIKWVKNHQEFISNI"
     gene            2473..2667
                     /locus_tag="P5241_02025"
     CDS             2473..2667
                     /locus_tag="P5241_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32323.1"
                     /translation="MADINSTQEEQEQSKSTVKEKNTGMAILAYILFFIPLLTDSKKD
                     PFVKYHVKQGLVLFIGGILI"
     gene            3125..3388
                     /locus_tag="P5241_02030"
     CDS             3125..3388
                     /locus_tag="P5241_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32324.1"
                     /translation="MPFSEGNTYIITVFGVENGKCHYALKMVDQTGALLSGGPGLLDC
                     SVPLEKISADTLGHFFGADQVEGQEGIKVVQDQIEKDYCITQQ"
     gene            3407..3646
                     /locus_tag="P5241_02035"
     CDS             3407..3646
                     /locus_tag="P5241_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRV32325.1"
                     /translation="MNKKIQMGACGICCSMCGLYIKNICTGCIKTKKNVEFLKSINAN
                     CPVLECAVNKKIDVCSKSCKKFPCNKFNGWPLTNE"
     gene            3853..4128
                     /locus_tag="P5241_02040"
     CDS             3853..4128
                     /locus_tag="P5241_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32326.1"
                     /translation="MKHNTIIWIFIIIGIILTSGYFYFINKNQNQLISCPLDAKICPD
                     GSAVGRIGAKCEFALCPGEQEGILVSTPKINEQIQSPLKIQGQAKGF"
     gene            4294..4710
                     /locus_tag="P5241_02045"
     CDS             4294..4710
                     /locus_tag="P5241_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32327.1"
                     /translation="MSDNPSGLSQNQKIFELSVQFKPSFSQSQQVLLYYYNPGKDKDT
                     NGNIKCSEDGLVAITKEIPITQTPIQDTIKLLLKGKENLTQQDIQNGITTEFPLPEFT
                     LIQANLSNAENGGILTLKFNDPLNKTNGGACRVKIL"
     gene            5425..5919
                     /locus_tag="P5241_02050"
     CDS             5425..5919
                     /locus_tag="P5241_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32328.1"
                     /translation="MSKTLVRQQKTFAKHDYLITPHSLIQFQHDSYEKFIKQGIKEVF
                     KDIFPVNDYSNEELSLDVTDYYLEEPKVDCETAKEKDINYESALHVNFKLTNKKTGEI
                     KEQEIYFGDIPTLTSTGTFIINGNERVVISQLTKSPGAYFAVTLHQTKNNLVKGSIIP
                     KRGA"
     gene            5950..6870
                     /locus_tag="P5241_02055"
     CDS             5950..6870
                     /locus_tag="P5241_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32329.1"
                     /translation="MNAKIDRRKKVPATTILKAFGVENNEEIKNIFSDIDTGDIQYIE
                     KTLSKDNTSNSNEALLDIYKKMRPKEPLALDNATSFFNNLFKRFDRYSFDKIGRYKFN
                     KRLNLPLNQVENKEFRVLHKEDIIAVLRELISMNNNPEATSDIIDHLGNRRVRSTGES
                     LQDRFFIAVFRMKRLIQDRMTMLDPTTLTPAQLISPRILTNAMRDFFNMSPLSQFMDQ
                     VNALSELEHKRRLTGSGPGGIKQKTKGGVEIRDIQPSHYGRICPINSPEGPNVGLVLN
                     LATFSRLNELGFLETPYFKVNHGKITKEIV"
     gene            6889..8406
                     /locus_tag="P5241_02060"
                     /pseudo
     CDS             6889..8406
                     /locus_tag="P5241_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            8422..8634
                     /locus_tag="P5241_02065"
                     /pseudo
     CDS             8422..8634
                     /locus_tag="P5241_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016447.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8823..9011
                     /locus_tag="P5241_02070"
                     /pseudo
     CDS             8823..9011
                     /locus_tag="P5241_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9090..9509
                     /locus_tag="P5241_02075"
     CDS             9090..9509
                     /locus_tag="P5241_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32330.1"
                     /translation="MGVSSLALEQVIYYNAYIITKINENELINIKNDINKEYKIELKN
                     ADNNQKSDINLKYKQALKELDIIHVKKVLNEKEYYYLTKKFSNAFEAETGSEPIVNFL
                     HTLDLQKEINDLQSKINKAPYSQKERLLKRLKILKSF"
     gene            9534..10367
                     /locus_tag="P5241_02080"
                     /pseudo
     CDS             9534..10367
                     /locus_tag="P5241_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012830.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4035 a         1464 c         1578 g         3304 t
ORIGIN      
        1 atatcggagt tggaagcgac gctaaaaaat tcgttctgca atcagtaggc agaggcgtaa
       61 gaatagaacc attaaaaggc aaaagaaaac gccttctcaa tttattcaac gcacaacaaa
      121 tagaaccaca attatttgaa cagataaaaa atttcgtttt gcctattgag agtttattta
      181 tttttggtac taatgccgaa aatctgaagg aaataattac aacgttgaaa acagagaagc
      241 aagataaaaa tttgggcgac gcgtttatat taaattctga agcgcaaaat catttgcttt
      301 tagtacccgt ttataaaact actgatagaa tttttgccga agaaaaagaa atacaaaaat
      361 atccggtgag cagagaggat ttcgatttaa cagcacaatt ttacagattt ataggcgata
      421 aagtcgctgt ggctaaatat aattgcgacg tgaaggtttt aaagaaaaca aaagaaagtt
      481 ttgagaaaaa agagcgatac tatgattttg gcgattatga ttttggcgag aaaaactcac
      541 tctttgagcc actctttgag ccagagttaa ttcttgaccg gatttttgat tatctcggag
      601 taaagagtaa agagtttgac aagtttaagg agttggaaaa tgaaatcgtg cacttcaaaa
      661 agattaggtt cagcggtggg gaaaaatatg aagaaatcaa acgaaaaata gaagaaatta
      721 gacaaaaacc agaaagagaa aaagaattaa aaaaaatatt taaaaaaggc aatcaaaaag
      781 aatacgatcg tcaaagaagt ttatttgaac aagcggagaa ttttgagatt aataaccaga
      841 aaataaaaat aaaatattta gccaaccatt actatcttcc agtgatagtt tcagaaaccg
      901 aaaaaattga ttatctcaat catattatta atgttaacag tgaggtacaa tttattgaac
      961 aactggaaga atatcttact caacctaata atgtatttac tcaatttgac tggtggatgt
     1021 tttcaaaaat tgaccaaacg atagatgaag tgtttatacc ttattataat cataagaaga
     1081 acggtatgga taattatcat cctgacttta ttttttggct acaaaaagga aatgattatt
     1141 tgatcttatt tgttgaccca catggaacta cttattcaga tataaataaa aaaatagatt
     1201 atttttctaa aatatttgaa gtaagagaga caaaagaaaa taaaattttc ccctctaatc
     1261 aatttggtat cacagaaaaa tttaatataa gagtaaaatt attgcttaaa ccagctcaag
     1321 gaggtttagt agctgttgga gaaaactatc aacaatactg atttgataat tttgaagatt
     1381 ttcagcaaaa attaaagatt tactaaaata ttatattatt ttaattgcta ttttataata
     1441 tagcgtaaaa tttattcaaa aatatgttta atcttacatt ttcatataat ctcaataaag
     1501 atcttgataa ttaccttaat gctatctata aatttaggta tttaaaacat ggaaggaaaa
     1561 atatagaaaa aaccgtaagt aagtatttat cacaacaaga actaactaaa attaaaacaa
     1621 gtaaaaatca aaaggcagcc actttagaaa tcaagaaaat cttattaaaa tggttagcaa
     1681 aaaatcagga tttaattaat ctaaaccaaa aaagtttaac agagagttgg aataatagac
     1741 aagataaatt tgttactttc tcggaagagt tttttgagaa aaaatttaat ttattaccag
     1801 caactgctta ctttactact ttaccaattt gtccttatta ttacccagat tggtttatga
     1861 taagtatcaa agagggttta gaaggacagc ttcataccat ttaccacgaa ttatttcatt
     1921 ttatgtttat caaacattac ggaaattatt gtaacaataa acacctaacc caattagaat
     1981 tcgaaagtat caaggaggca cttacggttt ttttaatgac gcctcctttc aataaaatca
     2041 atactctata tgaacaaggt tatcctcaag aacaagaatt aagacaattt atgctaaaaa
     2101 cgtatcaaca aaatcataaa tttatttctc ttctaaacaa tagcattaaa tgggtgaaaa
     2161 atcaccaaga attcatttct aatatttaaa aattaccctc tagtttaaaa atcttcatta
     2221 agtaataatt gaaaaataaa ataaaattaa ttaaatagaa caaatagtag gaaataaaat
     2281 tactgatttg ttgtatattt atattatatt aaaaccccca atatatacca catcttgatt
     2341 ttttttattt tttatagtat aattaaatta tctacactaa actacaaaga acaaaatcca
     2401 tatcaattta caatatatta caatataatt aaaggtcgag ctattattta tcaattgatt
     2461 aatttttaat ctatggcaga tatcaattcg actcaggaag agcaagaaca atccaagtct
     2521 actgttaaag aaaaaaatac aggtatggct attttggctt atattttatt ttttatacca
     2581 ctattaacag attctaaaaa agacccgttt gtgaaatatc acgtcaaaca gggtttagtc
     2641 ttgtttattg gcggtatttt aatatgaata cttggagcta ttcttccctg aggatttcgt
     2701 gtcataactc aaatactcaa ccttggttta tttgtgctaa tgattattgg cattttgaat
     2761 gcttccaagg gggagaaaaa acctttgcct attattggac aatttgctaa acaattaaaa
     2821 atataagaca cgatatttaa atatagaata caatattaac atatctaaac tatatttatt
     2881 acaaaataaa taaaaagcat tgcataatta taaaataaaa tattaattta acaatatgaa
     2941 aaaattatta atgattcttg ggattataat agttgtacta cttattattt gagttgtaat
     3001 acccaaaggt tccaacaacc aagaaacagt attagaacaa ccttctcagc aaggtcctat
     3061 tcaatgtgaa gaccccagtt gttttagtcc tcattttcta acttgtacac ccagtgaact
     3121 caaaatgcct tttagtgaag gaaatactta tataattaca gtgtttggtg tagaaaatgg
     3181 aaaatgtcat tatgctctca aaatggtaga tcaaacagga gctctattat caggaggacc
     3241 aggattgctt gattgctcag ttccattaga aaaaattagt gctgatacct taggtcattt
     3301 ttttggtgct gaccaagtag aaggtcaaga aggtatcaaa gttgttcagg atcaaataga
     3361 aaaagattat tgtattactc aacaataata actcaatact aatataatga acaaaaaaat
     3421 acaaatgggt gcatgtggta tctgctgttc catgtgtggt ctttatatta aaaacatttg
     3481 tacagggtgc attaaaacta agaaaaatgt agaattttta aaaagtatta atgctaactg
     3541 tcctgtttta gaatgcgcag taaataagaa aatcgacgta tgttcaaaaa gttgtaaaaa
     3601 atttccgtgc aacaaattta atggttggcc acttacaaat gaatgactta aaatgtataa
     3661 aaacagaata aaaaccaaaa aataatatta agaaaatttt ctgttgcata aacccataaa
     3721 ataaatatta tggcaaaaat aacaagctat gccaaaatat tttaagttag acaaaataga
     3781 tattataaga tattataatt gaaaacaact atttagtaaa caaaattagt aaacaaagtc
     3841 taatatttag ttatgaaaca caacacgata atctggattt ttattattat aggtattatt
     3901 ttaacaagtg ggtattttta ttttataaac aaaaatcaaa atcaactaat atcttgtcct
     3961 ttggatgcca aaatatgtcc tgatggttca gctgttggtc gtataggtgc aaaatgcgaa
     4021 tttgcacttt gccctggaga acaagaagga attttggttt caacgcctaa aataaatgaa
     4081 caaattcaga gtccattaaa aatacagggt caagccaaag gtttttgata ttttgaagct
     4141 cagtttaaag cagaactata tgatagtcaa aataatttat taggtcaagc aattttaaat
     4201 gcccaaacag attgaacttc tgaaaatttt gtaccttttt ctggcgaact tatatttaaa
     4261 caacctacaa caccaaatgg tattttaaga tttttgagtg ataacccatc aggattatca
     4321 caaaatcaaa aaatttttga actttcagta caatttaaac catctttttc tcaatctcaa
     4381 caagttttac tctattatta taatccaggc aaagataaag atacaaacgg caatattaaa
     4441 tgttccgaag acgggttagt ggcaataaca aaagaaatac ctataactca aacacctatc
     4501 caagacacaa taaagctttt gcttaaagga aaagaaaatt taactcagca agatatacaa
     4561 aatggaatta ctacagaatt tcctttgcca gaatttactt tgatacaagc caatttatca
     4621 aatgccgaaa acggtggtat tctaacttta aaatttaacg accccttaaa taaaactaat
     4681 ggcggtgctt gtagggtaaa aattttatga gctcaaattg aaaaaactgc caaacaattt
     4741 gctccggtaa aaatagttaa atttttgcct gaatcattat ttcaaccata attgaatgat
     4801 taattagtta tttttatgat actcaaaata tcttataaaa taagaggaaa atgaattacc
     4861 aaaagtatcc attttgaaaa acaagagaaa atgctcaaac ctcttgactt tttacaaaaa
     4921 tatgatatac ttaaatcata taaaccttta cgtctccaat tagaatatga acctggagaa
     4981 cgtaaaagca ttacctgtga aaggttggtt tcgattttaa acgacgtttt acacgtctca
     5041 caaaaaattt ttaaaaattg ctaaaattgt actattttga aatatcagtt aaaatagcat
     5101 ataaatcttt ataaaaatct cgaatgtgcg agggagtttt ttaatttgtc tattttttta
     5161 atctctattt ttaattttta ataatttata aacaaaaaag gggatggctg taaatatacg
     5221 atagtatcac tttgtatact tatcgtttaa tatcttttat ttatctaccc aaaattgatt
     5281 ttcaatatat ctattattat tcacttatag aagttttgta tcttgtatta aaatatatta
     5341 aaattatttt atttaatatc tctgaactta aactgggtat ataatataaa aaatacaacc
     5401 caaaccacta aactataaat aattatgtcc aaaacacttg tccgacagca aaaaactttt
     5461 gctaaacatg actatttaat taccccccac tctttaattc aatttcaaca tgatagctac
     5521 gaaaaattta taaagcaagg cattaaagaa gtcttcaaag atatttttcc tgttaatgat
     5581 tatagtaatg aagaattaag cttagatgtt acagattatt atttagaaga accaaaagtt
     5641 gattgtgaaa ctgccaaaga aaaagatatt aattatgaat cagctttaca tgtaaacttt
     5701 aagttaacta ataaaaaaac tggggaaata aaagaacaag aaatatattt tggtgatatt
     5761 cctacgctaa caagtactgg tacttttatt atcaatggta acgagagagt agtcatttct
     5821 cagcttacaa aatcgcctgg tgcctatttt gctgtaactt tacaccaaac taaaaataat
     5881 ttagtcaaag gttcgataat tcccaaaaga ggcgcttgat tagaatttat tactgaaaat
     5941 gacggtacta taaatgcaaa aatagatcgt cgtaaaaaag tcccagctac aacaatttta
     6001 aaagcttttg gtgtcgaaaa taatgaagaa attaaaaata tttttagtga tattgatact
     6061 ggcgatatcc aatatattga aaaaacactc agcaaagaca atacctcaaa tagtaatgaa
     6121 gctctcttag acatttataa aaaaatgcgt cctaaagaac cattagcgct cgataatgct
     6181 acgtcatttt tcaataattt atttaaacga tttgatagat atagctttga taaaattggt
     6241 agatataaat ttaataaaag attaaattta cccttaaatc aagtagaaaa taaagaattc
     6301 cgcgtattac acaaagaaga tattattgct gttttgcgtg aactaataag catgaataat
     6361 aaccccgaag ccacatctga tattattgat catttaggca ataggcgtgt tagatcaacg
     6421 ggagaatcat tacaagatcg cttttttatc gctgttttta gaatgaaaag acttattcaa
     6481 gacagaatga ctatgttaga tcccactact cttacccctg ctcaattaat tagtccgaga
     6541 attctaacta atgctatgcg tgatttcttt aatatgtcac ccctatctca atttatggat
     6601 caagttaatg ctttgtcaga gcttgaacat aaacgtcgtt taactggttc tggtcctggt
     6661 ggtattaaac aaaaaactaa aggtggtgtt gaaattcgtg atattcaacc aagccattat
     6721 ggcagaattt gtccaattaa ctcgcctgaa ggaccaaatg ttgggttagt attaaattta
     6781 gctactttta gccgcttaaa tgaattaggt tttttagaaa caccttattt taaagtaaat
     6841 catggtaaaa ttacaaaaga aatagtgtga tttgatgcta atgaggaaga gaatcatatt
     6901 attgctcgtg gggatgtgcc ttatgacaat aatggtaata ttttagaaca agaagtagag
     6961 tgccgtttta aaggtgatcc tcagatattt aaactctccg aaatagaata tattgatgta
     7021 tctccatatc agttattatc tcctacaagt gctttaattc cttttgtcga acgtaatgaa
     7081 gcccgacgtg ctttaatggg atcagttatg caaaaacaag ctgtccctgg cattattaaa
     7141 gataaaccat tagtagcaac tggtatggaa aaacaagtag cccgagattc tgggcaaatg
     7201 attttagcta aaaacagcgg tattgtaaaa gaagttgatg ccaatcatat acttatagaa
     7261 accaaagatc acaaaaaaga taattacccg ctcaaagtct ttgttcgtac taatcaaaat
     7321 acttgttttc atcaaacccc aattgttcat aaaaatgatg aaataaaaca aggacaagta
     7381 atcgccgaca gttcttctac ccaagatgga caattatcat taggacataa tttgttagta
     7441 gcatttatgc cttgatatgg ttatactttt gaagatgcta ttgttatttc agaaaatttg
     7501 cttaaacaag atgtttttac ttcagtttac acagaagaat ttatttgcga tcaacaagaa
     7561 actcagttag gaccagaaat aattacccga gacattccaa acatttctga aaaagcttta
     7621 agagatttag atgaagatgg tattattcgt cttggcgctg aagtagaagg taatgatatt
     7681 ttagtcggaa aaatatctcc aaaaggtgaa gtagaattaa ccccagaaga acgacttatg
     7741 cgcgctattt ttggtagtga atccaaagaa gtaagaaaca catctttatt tttaaaacat
     7801 ggacgccatg gtcgagtaat taaaactaaa attttgtcta gaaaaaaagg cgataaatta
     7861 gaggttggta ttataaaaag aatttttatt gaaattgccg aacttcgtaa agttaaagta
     7921 ggagataaat tagctggtag gcatggtaac aaaggtatta ttagtattgt ggctccagcc
     7981 gaagatatgc catttttaga agatgggact cctgttgata ttgttataaa tgccttatct
     8041 attccaaacc gtatgaatct tggtcaaatt tttgaatcac aattaggatt agctgctgat
     8101 agattacatt atcaagctat atgtcctcct tttgatggag ccacagaaga agaaataaaa
     8161 gaagaaatga ataaagctaa tattgataca aaaaacggag gtaaagttgc tctatttgat
     8221 ggtcgtacag gtcaaaaatt tgaacagctt gttactattg gtaaaattta tatgatgaaa
     8281 cttaatcata tggtagcgga taaaatgcat gcaagatcag taggtcccta tgctttaatt
     8341 actcagcaac cccttaaagg aagatcacaa atgggaggac aaagatttgg tgaaatggaa
     8401 gtatgagcat tagaagctta tggtgctgcc aaaactttgc aagagatgtt aactattaaa
     8461 tcagacgaca ttattagtcg tcgagaagcc tatgctgcaa taattaaaaa tgataaaatt
     8521 aaaagcccaa caatccccgc agcatttaat gttattttaa gcgaattaaa agctttgtgt
     8581 ctaaatattg aaatcaaaaa tattgcccca gaccaaaata caaaatctca ataacaataa
     8641 taacaataat gataaaacta tcaaaaatat aatatataaa aataattttt attaaataat
     8701 aatatttata aatttttatt atgttagaaa aaaatacaat gaatccatct gatgattttg
     8761 aaagtttaaa aatatcagtt gcttctccag aaaaaattgt cgagtgatcg cacggagaag
     8821 tttcagaagc tgaaacaata aattatagaa ctcaaaaacc agaaaaaggt ggattgtttt
     8881 gtgaagcaat ttttggtccc attaaagatt atcaatgtag ttgtggtaaa tatcgtggta
     8941 tacgcttcaa tgggataaaa tgtgatcgtt gtggagtggt tattactcac tcttctgtta
     9001 gacgtttttg aacaggtcat atatccttat gtattcctat ttgtcatatt tgatacctca
     9061 aaacccctcc tcatatatta gaggctgttt tgggtgtatc tagtttggct ttagaacaag
     9121 taatttatta caacgcctat ataatcacca aaattaacga aaatgaactt attaatataa
     9181 aaaacgatat aaacaaagag tataaaattg aattaaaaaa tgctgataat aaccaaaaat
     9241 ctgatattaa tttaaaatat aaacaggctt taaaagaact tgatattatt catgtaaaaa
     9301 aagttttaaa tgaaaaagag tattattatc ttactaaaaa atttagcaat gcatttgaag
     9361 cagaaactgg atcggaaccc attgttaatt ttttacatac attggattta caaaaagaaa
     9421 taaatgattt acaatccaaa ataaataaag ctccatatag ccaaaaagaa cgattattaa
     9481 agcgactaaa aatattaaaa tctttttgaa catccaaaac ccaaccagaa tgaatgttta
     9541 ttactaatct tccaatatta ccgcctgact tacgtccaat ggtcccgctt gatggaggaa
     9601 gatatgcatc ctcagattta aatgatcttt atagacgtat tattactcga aataatcgtt
     9661 taaaaaaatt aatgagttca aatgctcctt ttgttattgt aaaaaacgaa aaaagaatgc
     9721 tccaagagtc agttgatatg ttatttgata attctttgca tcaagggaga caaacttctc
     9781 aaagatctca aagaagacct ttacgatctt tggctgatat tctcaaaggt aaacaaggcc
     9841 gttttagaca aaatttactt ggtaaaagag tagattattc tggtcgttct gctattgttg
     9901 ttggacctac attaaaatta aacgaatgtg gagtaccaaa agaaatggct ttggaaattt
     9961 ttagaccttt tattattcat aaattattag ccaaagattt agctcatagt attcctgctg
    10021 caaataaatt aattgatgaa aaaaacgatg aagtttgagc tattttagaa gaagtaacaa
    10081 aagataaata tattttatta aatagagctc caactttaca cagattaagt attcaagctt
    10141 ttcatcctat attaatagaa ggactagcta tacaaattca cccaatggtt tgtgaaccat
    10201 ttggtgctga ttttgatggt gatactatgt cattacattt acctttgtcg gaagaagcac
    10261 aaaaagaatg tgacgatata atggtttcta cacgtggtct tttaaaacct tcaagcggag
    10321 aaacaaccat gagccctacc aaagatattg tcttagggtg ctattgacta acattagatt
    10381 t
//
LOCUS       DAOTJA010000020        10110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6952_length_10110_cov_6.715266, whole genome shotgun sequence.
ACCESSION   DAOTJA010000020 DAOTJA010000000
VERSION     DAOTJA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6952_length_10110_cov_6.715266"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..808
                     /locus_tag="P5241_02085"
                     /pseudo
     CDS             449..808
                     /locus_tag="P5241_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            1153..1716
                     /locus_tag="P5241_02090"
     CDS             1153..1716
                     /locus_tag="P5241_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32331.1"
                     /translation="MPARNEADVVAKTIYKLTTLNYPKNKFEIIIITDQKETLQKHKS
                     TQINTQINVDTTQEVVHKTIKELKHTKPEINIINLDVPYDFDGKFGGKCLGQEVKSTK
                     GRALNFALTELKEHFEEKTNFFAFFDTDDHPDSNCLLHIAKANLLDQTKKVFQLPVYQ
                     CRNFWHISAFSKVVCLSQSFTHENFLP"
     gene            1720..1860
                     /locus_tag="P5241_02095"
     CDS             1720..1860
                     /locus_tag="P5241_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32332.1"
                     /translation="MTWLPFLGGTNLYVQKDILFAVNGFNYNSITEDLDLGITIFLKT
                     KT"
     gene            2326..2463
                     /locus_tag="P5241_02100"
     CDS             2326..2463
                     /locus_tag="P5241_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32333.1"
                     /translation="MEGIVIPLVLFVYPLPFFVALVEHSLGMYKNKELVWVKTPRTQE
                     K"
     assembly_gap    2759..2768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2860..3504
                     /locus_tag="P5241_02105"
     CDS             2860..3504
                     /locus_tag="P5241_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV32334.1"
                     /translation="MIITWFGQSFFKIETQNKVIAIDPYDENYTGLKPARFKADILLV
                     SHQHQDHNNTKLILGEPFLLDSPGEIEIDGIFVEGIETFHDNNNGATLGKNTIFLIKS
                     EGLNIVHLGDLGEKKLKEELLETLGAVDILFIPVGGKYTINYSEAVNIVSQIEPNFVI
                     PMHYKIDGLKIDLDGIDKFTKEFGLTPEILDKFVIRKSNIPEEQDTKLIILQKQ"
     gene            3721..4299
                     /locus_tag="P5241_02110"
     CDS             3721..4299
                     /locus_tag="P5241_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRV32335.1"
                     /translation="MSVIISRAIPDSRDGLKPVQRRILYSMKTLGLTSDAKTRKSATI
                     VGETLGHYHPHGDIAVYDALVRMAQDFSLRMPLVKGQGNFGCFTGDTKVALVDGRNLN
                     FKDLIKEQKLGKKHLTYTFNLKKNKIEITEILKPRLTRKNAKLIEITLDNNEKIKCTL
                     NHKFLMRNGQYKEAQLLKTGDSLMPLYIDNYN"
     gene            4441..4581
                     /locus_tag="P5241_02115"
     CDS             4441..4581
                     /locus_tag="P5241_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32336.1"
                     /translation="MTINNIFKHFDRLEQIPTETKKYNHKVIQIKILKTKEDVYDITT
                     KP"
     gene            4594..6252
                     /locus_tag="P5241_02120"
     CDS             4594..6252
                     /locus_tag="P5241_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRV32337.1"
                     /translation="MASGVFVHNSIDGDPPAAQRYTEAKLASISDYLFEDIDKDTVNF
                     IPNFDGTCTEPAVLPARFPQLLINGTTGIAVSMATNIAPHNISEVMDAVAYLINYPDA
                     GVEDLMKFIKGPDFPTGGIIYGIENIKTAYATGKGKVLIRGKTEIVENNKKTQIIITE
                     IPYMVNKSEMIKHIANLVENKKIIGIKDLRDESDKEGLRITIDLKSDIRPQSVLNQLY
                     KYTELEKYFYINQIALTDNGLQPRLLTLKDLLEDFINHRKEVVLRRTRFLLKKYKDRL
                     HILEGLSTALDNIDEIIQIIKKSENREDALNNLCKAFKFTEVQANAILEIKLQTLAKL
                     EKYKINEEIKEKQGLIKECQLILKDEKELFLVIQNEIKEIKEKFVERRRTEIVGHLPE
                     EVSSIDLIPEENVLITLSKSGYIKRMNPDILRTQQRGGKGVIAFEGQNSEDFLAKIIS
                     ANTKDYLLFFTDKGKVYQSLVYDINESSRSAKGKAIQNFINIDPSETITAVLSYNPQK
                     TGNHRFLIMATKNGIIKKTKIDEFANIRKTGLVAINLEKNDILQ"
     gene            6286..6735
                     /locus_tag="P5241_02125"
     CDS             6286..6735
                     /locus_tag="P5241_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRV32338.1"
                     /translation="MVTQNGQSICISEKDIRAMGRATSGVCGIKLNKDDFVIGLNIVP
                     AKIDKNTSTLLTVSEMGYGKRTLIKEYRIQKRGGTGIKTFKVTDRTKKLVASRLITTQ
                     TTLITISKKGLILKTELADVSIQGRTTQGVKLMRLDPGDTISEIETF"
     gene            6901..7056
                     /locus_tag="P5241_02130"
     CDS             6901..7056
                     /locus_tag="P5241_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32339.1"
                     /translation="MSGLLNPNEVVKKLPVIPSMVIADFGAGSGFFSIALAKALNNNG
                     KVIALDI"
     assembly_gap    8111..8210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8394..8534
                     /locus_tag="P5241_02135"
     CDS             8394..8534
                     /locus_tag="P5241_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32340.1"
                     /translation="MASRFNYNKKTISVEYTEKNPDTYLQELDTALKEGDAPDIFMIN
                     NL"
     gene            8559..8750
                     /locus_tag="P5241_02140"
     CDS             8559..8750
                     /locus_tag="P5241_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32341.1"
                     /translation="MPLDLIDKNYNINNIRKEFVPVVEQDCVEGNNLFGLPISVDTLA
                     LYYNVNILDQLGIANIPST"
     gene            8814..8999
                     /locus_tag="P5241_02145"
     CDS             8814..8999
                     /locus_tag="P5241_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32342.1"
                     /translation="MAIPLGLADNINHAADILSALMLQQGLDIINKTTRTSDINNGIE
                     ALNFYTQFAKPSSLFYT"
     gene            9021..9221
                     /locus_tag="P5241_02150"
     CDS             9021..9221
                     /locus_tag="P5241_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32343.1"
                     /translation="MDSFAFGQSVFYIGYLRDAELIKEKNPNLIFDIAEFPQSSKQNN
                     KTTFASYNVLVVSKQSTNPQIA"
     gene            9324..9506
                     /locus_tag="P5241_02155"
     CDS             9324..9506
                     /locus_tag="P5241_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32344.1"
                     /translation="MPLMSAFAKGALVAKTFYCPDYTQYEYLFNDTITNVALKNMPVK
                     DALDKLQTNLSYQLYK"
     gene            9512..9775
                     /locus_tag="P5241_02160"
     CDS             9512..9775
                     /locus_tag="P5241_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32345.1"
                     /translation="MKKFIILVIFSSLLISLLVPTFIFAKGLVPCGGKGEHACTACDI
                     FVLIQNIMNLVFEYSFVIAFVLIIIAGFNMMFSNGNSKKIQGA"
     gene            complement(9763..9960)
                     /locus_tag="P5241_02165"
     CDS             complement(9763..9960)
                     /locus_tag="P5241_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32346.1"
                     /translation="MPPSPSDVFSVLGATVPETHSNLYQLRCNLGSPGAKKYKTVFTT
                     TQAPKISTNPIRTFCILFQAP"
BASE COUNT         4160 a         1376 c         1440 g         3024 t
ORIGIN      
        1 aaaaaataaa aggaagtgat cataatcaaa ataaaataaa gatcaaataa gggaggtaag
       61 acaaaatcaa aataatcaaa acaaaaaata aacaaatact atgaaaaaat ataaaattac
      121 acaagataga aataaatgct tagggtgtgg taggtgcact atggtgtgcc cagaaaattg
      181 agagattgat gaaaaagatt ttttagcaaa accgaaaaaa gatattattg atgaaactga
      241 attaaactgc aataaaatag ccgaagaaga atgcccgagt aaatgtatca atattacgca
      301 aattaacgcc taaaacgcga attaatacga atatgacaaa gatatagatt tacacaaata
      361 ataaaagcca tttttgttag tgtaaacttc taattttcaa tttccatttt ttattaatta
      421 aaaattatat taaaataaat aataaattat gttagatcaa gattgtgtgt tttgtaaaat
      481 tattagaaat gaaataccaa gcaaaaagtt ttatgaagat gaaaactttt tggctattta
      541 tgatattcag cctataacag aaggccatat tattttgatg tcaaaaaatc actataccaa
      601 taccaatgaa atgccgagcg atgaatgatt tcgttttatg ataaaagccc gagatttggc
      661 tgaggactta gtacgcgctt atgctgctga tggctacaat ttattaatta atcagggcga
      721 agccgcacaa tcaggtgtag cccatagacc acattgtcat attatacccc gaagaaaagg
      781 agatctaata aaaatagacc ctcgctaaca ctaatatgta gatttgttca gatcaaaagc
      841 aatttctacg actttcaatt ttcaattatt aaaaactgat aaatacgata aacaattcta
      901 atgtctatat tccaattatt ttcaacttat ttacaatatt atttgccaat aaatttctac
      961 aattttttaa atttaacatc gagttattga actaaaataa taggcgcaat agcaattatt
     1021 ttgttggggc gcactattat tgtatttttt cgctcacaaa aaaaatgacc gaaaaatcat
     1081 actattaata taaacgacat tcaaaatatt gcaaaaacaa aaaataaacc actcccatat
     1141 ttttctatat tagtgccagc aagaaacgaa gcagatgtag tagccaaaac tatttacaaa
     1201 ttaactactc taaattatcc caaaaataaa tttgaaatta ttattattac tgaccaaaaa
     1261 gaaacattac aaaaacacaa atcaacacaa ataaatacac agataaacgt agatacaaca
     1321 caagaagtag tacacaaaac aataaaagaa ctaaaacata ccaaaccaga aataaatata
     1381 atcaatttag atgtacctta tgattttgat ggtaaatttg gaggaaaatg tctaggccaa
     1441 gaagttaaat caacaaaagg tagggctcta aattttgctt taacagagct aaaagaacat
     1501 tttgaagaaa aaacaaattt ttttgctttc tttgatacag atgatcaccc agatagcaat
     1561 tgcttattac acattgccaa agctaattta ttagaccaaa caaaaaaagt ttttcaattg
     1621 ccagtatatc aatgtagaaa tttttggcac atttcagcct tttccaaagt agtatgtttg
     1681 agccaatctt tcacccatga aaatttttta ccttgaatta tgacctggct accatttttg
     1741 ggtggtacaa atttatatgt tcaaaaagat attttatttg ctgtaaatgg ttttaactat
     1801 aattcaatta cagaagattt ggatttgggt ataacaatat ttctaaaaac aaaaacctga
     1861 ccacattttt taccagtgcc atcttccgaa caaacaccac caaatatgaa agcttatttt
     1921 aagcaaagaa aaagatgagc aatgggacaa ttagaagtta tggacaattt gaaaaatatg
     1981 atcaaaaata aagagggtga tcctaaaatt ataaaagatt tatacaaaaa attatgacta
     2041 tatggccctg ctgaatctac tttatttttt ttactgacaa tttcgagctt tttggtgctt
     2101 gtctcgcgaa taataaaggg aatattttta attattacac tacaatcttt ggctgtttat
     2161 ttttctccag cttttacttt acgggaaact ttatatgcca tggcatctat agttggtatt
     2221 ccaatgatta tattttcact aattttattg catagatata aaaaatatat tgaaacgcca
     2281 aaatcaaaat tgtttcaagc ttgagagcta ataaaattta tattaatgga aggcattgtg
     2341 attcccttag ttttatttgt ataccccttg ccattttttg tggcattagt agaacatagt
     2401 ttgggaatgt acaaaaataa agaactggtt tgggtcaaaa caccaagaac tcaggaaaaa
     2461 tagatatcca tatttttccc taattatgat tatatcttgt ttatgtctta aatttcttaa
     2521 atcaaaatct ctttaaaatt cttaatattt attttagtgt ttgttttgca aaaaagccaa
     2581 tccaattttt gagtcatatt caataaaaat tgatattata tactatttat tgagatatca
     2641 ataacctatt gtattatttc taattttatg ataaaatact aatatattat aagactataa
     2701 tcataatttt ttctacaatt aaaattttgt tttctgtatt gatgattttc acgctttcnn
     2761 nnnnnnnnaa cctaaaaatc aaaattttta agatttgaat tatggatttg agatttgact
     2821 cttgagattt aagattaaaa aatttactaa ttttttatta tgattattac atggtttgga
     2881 caatcatttt ttaaaataga aacacaaaat aaagttattg ctattgatcc ctatgatgaa
     2941 aattatacag ggcttaaacc tgcccgtttt aaggctgaca ttttattagt atctcaccaa
     3001 caccaagatc acaataacac aaaacttatt ttgggagaac cttttctatt agatagccct
     3061 ggagaaattg aaatagacgg gatatttgta gaaggtatag aaacattcca tgataataat
     3121 aatggcgcaa cattaggcaa aaatactatt tttttgatca aatccgaagg tcttaatatt
     3181 gtacatttag gagatttggg cgagaaaaaa ttaaaagaag aattattaga aacattgggg
     3241 gcagtagata ttttatttat tcctgtgggg ggcaaataca ctataaatta ttctgaagct
     3301 gtaaatattg ttagtcaaat agaaccaaat tttgttattc caatgcatta taaaattgat
     3361 ggattaaaaa ttgatttaga tggcatagat aagtttacaa aagaatttgg cctaacacca
     3421 gaaatattag acaaatttgt tattcgtaaa tctaatattc cagaagagca agatacaaaa
     3481 ttgataattt tgcaaaagca ataaatattg ttgctactta tatattttac atgctaaata
     3541 ttatatatga ccaaaaaaca caaccaacaa caaaacaata aaactcccaa accagaagca
     3601 gaaaaacaaa aaacaaaaaa tacttcacaa ctacaagatg aagaaaatct tacaactgct
     3661 aatacaataa tagagcgcga tataaatgac gaattacgtg aatcattttt agattacgcg
     3721 atgtctgtta ttatttctcg agctattcct gatagtcgcg atggactaaa acctgtacaa
     3781 agaagaattt tatattcaat gaaaactttg gggctaactt cagatgccaa aacaagaaaa
     3841 tcagcaacta ttgttggtga aactttggga cattatcatc ctcacggaga catagctgta
     3901 tatgatgctt tggttagaat ggcacaagat ttttctttac gtatgccact tgtaaaagga
     3961 cagggaaatt ttggttgttt tacaggagat accaaagtag ctctggtaga tggtagaaat
     4021 ttgaatttta aagatttgat caaagaacaa aagctgggca aaaaacactt aacttatact
     4081 tttaatttaa agaaaaacaa aatcgaaata acagaaatac taaaaccaag attaacaaga
     4141 aaaaatgcca aactaataga aataactttg gataataatg aaaaaattaa atgtacttta
     4201 aaccataaat ttttgatgag aaatggacaa tataaagaag ctcaattatt aaaaacagga
     4261 gattcgttaa tgccattgta tattgataac tataattgac aaaatagaga ctttaaaaat
     4321 tctaaaagaa tctggcaacc tataacacac caatgagaat ttattcagca attagctgat
     4381 aactgacaat tagttacaaa aaataaatac cataaaaatt ttgataaatg aaatggtgtt
     4441 ttgacaataa acaatatttt taaacatttt gatagattgg agcaaatccc cacagagacc
     4501 aaaaaatata atcataaagt aatacaaatc aaaattctca aaacaaaaga agatgtatat
     4561 gatattacca caaaaccttg acacaatttt gctttggcta gtggtgtatt tgtccataat
     4621 tctattgatg gcgatcctcc agctgcccaa agatatactg aagcaaaatt agcttcgatt
     4681 tctgattatt tatttgaaga tatagacaaa gatacagtga attttatacc caattttgat
     4741 ggcacctgta cagaaccagc agtattacct gctcgttttc cacaattact gataaatgga
     4801 acaactggca ttgctgtatc aatggcaacc aatattgccc ctcataatat ctcagaagtt
     4861 atggatgctg tggcatattt aattaattat ccagacgcag gagtagagga cttaatgaaa
     4921 tttatcaagg ggccagattt tcctactgga ggtattattt atggcattga aaatatcaaa
     4981 actgcttatg ccactggcaa aggcaaagtt ttgattaggg ggaaaaccga aatagtagaa
     5041 aataacaaaa aaactcaaat tattattaca gaaatcccct atatggtaaa taaatcagaa
     5101 atgataaaac atatagctaa tttggtagaa aataaaaaaa ttattggcat taaagattta
     5161 cgcgatgaat cagataaaga aggactgcgt ataacaattg atctaaaatc tgatataagg
     5221 ccacaatctg ttttgaatca gctttacaaa tatactgaat tagaaaaata tttttatatc
     5281 aatcaaattg ctttaactga taatggttta caaccaagat tgctaacatt aaaagattta
     5341 ttagaagatt ttattaatca tagaaaagaa gtagtattac gtagaactcg gttcttgtta
     5401 aaaaagtata aagacagatt acatatatta gaaggactat caactgctct tgataatatt
     5461 gatgaaatta tacaaataat taaaaaatca gaaaaccgag aagatgctct gaataatttg
     5521 tgtaaagcat ttaaatttac agaagtccaa gccaatgcta ttttggaaat taaattacaa
     5581 actttggcta aattagaaaa atataaaatc aatgaagaaa tcaaagaaaa acaagggctt
     5641 atcaaagaat gccaattaat tctgaaagat gaaaaagaat tgtttttggt tatacaaaac
     5701 gaaatcaaag aaattaaaga aaaatttgta gagcgcagac gcacggaaat tgtcggacat
     5761 ttgccagaag aagtatcatc tattgattta ataccagaag aaaatgtttt aataacatta
     5821 tcaaaaagtg gatatataaa aagaatgaac cctgatatat tgcgtactca acaaagaggc
     5881 ggcaaaggag taatagcttt tgaaggacaa aactctgaag atttcttggc aaaaattatt
     5941 tcagccaaca caaaagacta tcttttgttt tttacagata aaggcaaagt ttaccaaagt
     6001 ttagtttatg acataaacga atcttcacga agtgctaaag gcaaagcaat acaaaatttt
     6061 atcaatattg atccaagcga aacaataaca gcagtattga gctataatcc acaaaaaact
     6121 ggcaatcata ggttcttgat aatggcaaca aaaaatggta taataaagaa aaccaaaata
     6181 gatgaatttg ctaatataag aaaaacggga ttggtagcta taaatttaga gaaaaatgat
     6241 attttacaat gaacaagttt ttcatcaggt gatgactatg ttattttggt aacacaaaac
     6301 ggacaatcta tttgtatttc tgaaaaagat ataagagcga tgggaagggc aacttcgggg
     6361 gtatgcggta ttaaattaaa taaagatgat tttgtaattg gtctaaatat tgtacctgca
     6421 aaaattgaca aaaatacatc tacactatta acagtaagcg aaatgggtta tggtaagaga
     6481 actttaatta aagaatatag aatacaaaaa cgtggaggta ctggtataaa aacattcaaa
     6541 gttactgata gaactaaaaa attagtagct tcgcgattaa ttactactca aacaactcta
     6601 ataacaattt ccaaaaaagg tttaatacta aaaactgaat tagccgatgt gtctatacaa
     6661 ggaagaacta cccagggtgt aaaactaatg cgattagatc caggagatac aatatcagaa
     6721 atagaaactt tctaaatact aataaaacac taataaatat aaacacagat acatactaat
     6781 ctaaacacaa cagatataaa taaaaaagat aaaataattt ttgttcttat caacttctaa
     6841 tccctaattt caaactctca ttagcaaaac attaaaaatt attaataatt attatatatt
     6901 atgagtggtt tattaaatcc taatgaagtg gtaaaaaaat taccagtaat accatctatg
     6961 gtcatagccg attttggtgc tggatctgga ttttttagta ttgctttggc aaaagcttta
     7021 aacaataatg gcaaagtaat tgctctagat atttgaccaa aggctttgga agcattgcaa
     7081 acaagggcaa agatagaaaa agtttgacca gttatagaaa caaaaatagc tgatttagaa
     7141 aaaccgaacg gctcaaaact aacatcagaa agcgtaaata tggtgctaat ttctaatatg
     7201 ttgttccaaa cagaaaataa aataaatatt ctaaaagagg caaaaaggat attgcaaaaa
     7261 gatggttttc ttgttataat agattgagac cctcaaaagc tacctaataa agaaaattta
     7321 ttccccatag ataaaaataa aataaacaaa acaattttgg atatgggctt taaattcaag
     7381 gaagatattt ttatttctga cactcattat tgtttaatat atattaaaaa ttaaatataa
     7441 aataagaaca aataaggaaa aattaaaata aaaaatacaa aataagggaa aaataaaata
     7501 aaatataaaa aataagggga aaacaaagac catctaataa agccataatc acaataaaat
     7561 aagaacaaat aatagaacac aatcataatt aaaataaaag ataacatacg ggaaaatcaa
     7621 atacaataaa aacaaaataa aagatataaa atggctgata taaattcaca aaatacccaa
     7681 acacgtttta catcaaaaca aaaaataacc aaaacaaaat aaaacacaat aagagaacaa
     7741 caaagaccat ctaataaagc cataatcaca ataaaataag aacaaaaaag gaaaaattaa
     7801 aataaaacaa aataagggaa aaacaaaata aaatataaaa aatggctgat ataaattcac
     7861 aaaataccca aacacgtttt acatcaaaac aaaaaataac caaaacaaaa taagacacaa
     7921 taagagaaca acaaagacca cctaataaag ccataatcac aataaaataa gaacaaataa
     7981 tagaacacaa tcataattaa aataaaagat aacatacggg aaaatcaaat acaataaaaa
     8041 caaaataaaa gatataaaat ggctgatata aattcacaaa atacccaaac acgttttaca
     8101 tcaaaacaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctgatataa
     8221 attcacaaaa tacccaaaca cgttttacat caaaacaaaa aataattata ggcagtattg
     8281 ttgtagtgct gtttattatt attgtatttg gaggcagttt tatgaaaaaa tttacagcta
     8341 ctcccacatt aaaagtatga ggtattggta taaacaatga tgattggtca cgtgtggcat
     8401 cgcgatttaa ttataataaa aaaacaataa gcgttgaata tacagaaaaa aatcctgaca
     8461 cttatttgca agaacttgat actgccttaa aagagggcga tgcacctgac atatttatga
     8521 ttaataattt atgaatacct tcttacaaaa atatcattgt gccattggat ttaatagata
     8581 aaaattacaa tatcaacaat attcgcaaag aatttgtccc agtagtagaa caagattgtg
     8641 tagaaggaaa taacctattt gggctaccta tttcagtaga tacattagct ttgtactata
     8701 atgtaaatat tttagaccaa ttgggcatag ctaatattcc aagcacctga aatgatataa
     8761 ctaataatat ctctatatta aggactgttg accaatataa tcgtataaaa cgagtggcaa
     8821 tacctttggg actagcagat aatatcaacc atgcagcaga tattttatca gcgcttatgt
     8881 tacaacaagg tttagatatt ataaacaaaa ctactcgaac ttctgatatt aacaatggta
     8941 tagaagcttt aaatttttat acacaatttg ctaaaccttc ttctttattt tatacttgaa
     9001 acagcgagtt taccaattct ttggattctt ttgcttttgg acaaagtgtg ttttatatag
     9061 gatatctaag agatgcggaa ttaataaaag aaaaaaatcc aaatcttata tttgatattg
     9121 ccgaatttcc ccagtcttct aaacaaaata acaaaactac ctttgcctct tataatgttt
     9181 tggtagtttc taaacaatca accaaccctc aaatagcttg agcatttata aaatatctaa
     9241 ctaccgatga tattacagat caaataacta gtcaagccaa tttattacca agcaaaagat
     9301 ctttaataga atactatatt aatatgccct tgatgtctgc ctttgccaaa ggagccttgg
     9361 tagcaaaaac tttttattgc ccagattata ctcagtacga atatttattt aatgatacaa
     9421 ttactaatgt ggcgttaaaa aatatgccag tcaaagatgc gttggataaa ttacaaacaa
     9481 atttatcata tcagctttat aaataaataa tatgaaaaaa tttattattt tggttatatt
     9541 tagcagtttg ttgattagtt tattagtacc aacttttata tttgccaaag gattagtacc
     9601 ttgtggtggt aaaggggaac atgcatgtac tgcctgtgat attttcgtat taattcaaaa
     9661 tattatgaat ttggtatttg aatattcttt tgttattgcc tttgttttaa ttataattgc
     9721 tggatttaat atgatgtttt ccaacggaaa ttctaaaaaa attcaagggg cttgaaatag
     9781 tatacaaaat gtccttattg ggttagtact tattttggga gcctgagtag ttgtaaatac
     9841 tgttttatat ttctttgctc ctggactacc aagattacaa cgaagttgat ataaatttga
     9901 atgtgtttca ggcactgtag cacctagcac agaaaataca tccgaaggtg aaggtggcac
     9961 ttttatacca ggagcaggtg gagaaggtgg tggaggaagt ggtggtggta tggattaaat
    10021 atataataca agtaaaaaat ataatttaaa taataactaa ataatacaaa acaaaaatat
    10081 aagaaaatac cgacaaataa aagaaaaaat
//
LOCUS       DAOTJA010000021        10063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_6991_length_10063_cov_6.950040, whole genome shotgun sequence.
ACCESSION   DAOTJA010000021 DAOTJA010000000
VERSION     DAOTJA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_10063_cov_6.950040"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1041
                     /locus_tag="P5241_02170"
     CDS             226..1041
                     /locus_tag="P5241_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32347.1"
                     /translation="MTKKTTNSTIQKQIVSKYPDIQVIRHSLAHIMALALQRLYKNVQ
                     FGVGPDIDNGFYYDVFTKKPITIEDLPKIEAEMKNIIKENLPFSKFELSINNGKNLFK
                     KLNQKFKLELIDDLKNFGTTNVSEIEKLKDLPKKKIKGDKSISVYVLGENIKKAQAKS
                     KHTTNVLFTKKDLLKLQDKNKVFVDLCRGPHIKSTKDVPDTFKLVNVAGAYWRGNEKN
                     KMLTRIYGVAFLDKQEMDEYSNMLIEAEKRDHKKLGLALDIFFLDDTAPGMPY"
     gene            1042..1173
                     /locus_tag="P5241_02175"
     CDS             1042..1173
                     /locus_tag="P5241_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32348.1"
                     /translation="MPNGVIILNELLDFWRKEHKKRGYLEIMSPLLNKKQLYEISGH"
     gene            1192..1557
                     /locus_tag="P5241_02180"
     CDS             1192..1557
                     /locus_tag="P5241_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32349.1"
                     /translation="MFIIEDKNQGKNPDIYCLKPMNCPNAMIVYKHQTHSYRDLPLRY
                     SDCDVLHRNEKSGELSGLLRVSKFIQDDAHIFVSLSQIKSEIQNILDIAKLFYSVFNI
                     KFQLRLGTRPKDFMGNKKD"
     gene            1708..1863
                     /locus_tag="P5241_02185"
     CDS             1708..1863
                     /locus_tag="P5241_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32350.1"
                     /translation="MDFQIPKNFGLTYTDSDGSLKTPIVIHRVIYGSLERFMGILIEH
                     FAGAFPL"
     gene            1984..2172
                     /locus_tag="P5241_02190"
     CDS             1984..2172
                     /locus_tag="P5241_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV32351.1"
                     /translation="MSKRIRNAELQKIPYIVVVGDKEQEHSIIAVRERFVGDLGAFSI
                     EDFISRLIREIEDKIIKN"
     gene            2271..4715
                     /gene="leuS"
                     /locus_tag="P5241_02195"
                     /pseudo
     CDS             2271..4715
                     /gene="leuS"
                     /locus_tag="P5241_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
     gene            complement(5046..5639)
                     /locus_tag="P5241_02200"
     CDS             complement(5046..5639)
                     /locus_tag="P5241_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV32352.1"
                     /translation="MVCEDCGYVFKCSNCDVPMIYHKENNINLLTCHHCGQEMLAPTL
                     CEHCQGGNLRMIGLGIERVEDELKHLSKKQSQTNKTFTIKRIDSEIPEDKIRQIIQDF
                     NNHKFDILLGTEIILRPQLNPVSLTAVVSIDAMFTFPDFRQEEKILKYLLKLKVKATN
                     NFIIQTMFRDIALFQAIKNNTVTDYLKQNLITKKHYL"
     gene            complement(5649..5855)
                     /locus_tag="P5241_02205"
     CDS             complement(5649..5855)
                     /locus_tag="P5241_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32353.1"
                     /translation="MAKKLKIPLIYNNLENTTTTNPKTNIFIYNPYINSPKKTQYLSS
                     LLKKEILKTINNNEQAILFLNRKG"
     gene            complement(6021..6176)
                     /locus_tag="P5241_02210"
     CDS             complement(6021..6176)
                     /locus_tag="P5241_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32354.1"
                     /translation="MSSNNQIPTTLIACPTEAHLDFIVSTLLQTYDSSQITIIPKKLY
                     QKDFNKA"
     gene            complement(6348..6548)
                     /locus_tag="P5241_02215"
     CDS             complement(6348..6548)
                     /locus_tag="P5241_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32355.1"
                     /translation="MYLLDVLPLTNLPKSESQIMSYYYTEDLTQGAIVEIDLNHRLIL
                     GLVINSTPIKTVKTFLKNADYK"
     gene            complement(6877..7158)
                     /locus_tag="P5241_02220"
     CDS             complement(6877..7158)
                     /locus_tag="P5241_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRV32356.1"
                     /translation="MYNNHRLFIAIPLDNTNQNKLVQFQQTHLNPSVFNIVSKDNLHL
                     TLVFLGNNDNNAILQISQILNKIAHHFNPFTIILDKIEYGPTPNRQRLV"
     gene            7382..7465
                     /locus_tag="P5241_02225"
     tRNA            7382..7465
                     /locus_tag="P5241_02225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7416..7418,aa:Leu,seq:caa)
     gene            7686..8915
                     /locus_tag="P5241_02230"
     CDS             7686..8915
                     /locus_tag="P5241_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRV32357.1"
                     /translation="MLSSGEQEQNNNIISSPSSQDSQNILEMSDQEPNIASLSSVVTS
                     TEPLSISQPIKDDKANLLVNTSLLNTDININNNLDVNDKSLENTLNLVKDDIMSKDII
                     SEVNNQEQSANVDFLKTEMHDRIFQLEVNKIKPNPYQPRKEFDPKSIEELAASINEYG
                     ILEPLIVTRNEIETDKGLDVEYQLIAGERRLRAAQFLGLKTVPAIIKTLKNEVAKLEL
                     ALVENLQREDIDSIARAKSYAKLINEFGYTQEMVAQKVGKSRESIANTLRLLQLPFEA
                     QRAIQENKITESHARALLLLPNMEKQRALLGEILTKQLSSREADLIARQFLEQQVGKN
                     SQSRKRLGGSFDPMDLELREKLESVFDTKVLIKRKGQRGEITIHFDSNDELNGLVDKM
                     LKLSEKRVNNTAGKISL"
     gene            8931..10055
                     /locus_tag="P5241_02235"
                     /pseudo
     CDS             8931..10055
                     /locus_tag="P5241_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
BASE COUNT         3623 a         1098 c         1779 g         3563 t
ORIGIN      
        1 gaagagtata aggggtatta gtctatactt cttactttta tattccgata tatcaaatat
       61 attcaaaaac ttggtttttg aatattagat cagattgttt tgacagttta tatataaact
      121 gtcaaaacca tatcaataca tacaaatgta tacaaagtct tgcagaaatt tatattttat
      181 gctatattta tagtaagttt tatatttaat tgttaaacat aatttatgac caaaaaaaca
      241 acaaactcaa caatacaaaa acaaatagtg tcaaaatatc cagatattca agtgatccgt
      301 cattctttgg cgcatataat ggctttggct ttacaaagat tatataagaa tgttcagttt
      361 ggagttggtc ctgatattga taatggtttt tattatgatg tttttaccaa aaaacctatt
      421 actattgaag acttgcccaa aattgaagca gaaatgaaaa atattattaa agagaatttg
      481 cctttttcta aatttgagtt gtctattaat aatggtaaaa atttatttaa aaaattaaat
      541 caaaaattta agttagaatt gatcgatgat cttaaaaatt ttggtactac taatgtttca
      601 gaaatagaaa aattaaaaga tttacctaaa aagaaaatta aaggagataa aagtattagt
      661 gtttatgtat taggagaaaa tataaaaaaa gcacaggcta aaagtaagca tacaacaaat
      721 gtattgttta ccaaaaaaga tttattaaaa ttacaggaca aaaacaaggt gtttgttgat
      781 ttgtgtcgag gaccccatat caaaagtaca aaagatgttc cagatacatt taaattagta
      841 aatgtggcgg gtgcttattg gcgaggaaat gaaaaaaata aaatgttaac tagaatttat
      901 ggcgtagctt ttctggacaa acaagaaatg gatgaatatt caaatatgct tatagaggca
      961 gaaaagagag atcataaaaa attaggtttg gcattagata ttttttttct agatgataca
     1021 gctccgggta tgccttattg attgcctaat ggtgtgatta tattaaatga acttttagat
     1081 ttttggcgta aagagcataa aaaaagaggt tatttagaaa taatgtcgcc tttgttgaat
     1141 aaaaaacaat tatatgaaat ttctggacac tgagaccatt ataaagacaa gatgtttatt
     1201 attgaagata aaaatcaggg taaaaatcca gatatttatt gtttaaagcc aatgaattgt
     1261 ccgaatgcta tgatagtgta caaacatcaa acgcattcat atagagattt accattaaga
     1321 tattccgatt gtgatgtttt gcatagaaat gaaaaatctg gagagttgag tgggttgttg
     1381 cgtgttagta agtttataca agatgacgcg catatatttg ttagtttatc gcaaattaaa
     1441 tcagaaattc aaaatattct tgatattgcc aagttgtttt attctgtatt taatataaaa
     1501 tttcagttga gattaggtac aagaccgaaa gattttatgg gtaataaaaa agattgagat
     1561 aaagcagaag aagatttaaa agatgtttta gataaatcag gtttatatta ttatattggg
     1621 aaaggggaag gtgcttttta tggtccaaaa attgatatta tgatgcaaga ttgtttaaat
     1681 agagattgac agcttggaac aattcaattg gattttcaaa ttcctaaaaa ttttggttta
     1741 acttatactg attctgatgg gtctttgaaa actcccattg ttattcatag agttatttat
     1801 ggatcattag agaggtttat gggtattttg atagaacatt ttgctggtgc ttttccttta
     1861 tgattgtcgc caatacaaac ggttatattg cctgtgggcg ggaaaaatat agaatatgct
     1921 aatagtataa atatgtcatt aaaagcaaaa aatattcgta gtgaagtttg aagcaacgaa
     1981 actttgtcta aacgtattag aaatgctgaa ttacaaaaaa tcccttatat tgtggtagtt
     2041 ggagataaag aacaagagca tagtattatt gcagtaagag agagatttgt gggcgatctc
     2101 ggagcttttt caatagaaga ttttatttca cgtttgatta gagaaattga agacaaaatt
     2161 attaaaaatt aatatttaaa aattaatatt taataattaa tatagagtag gtaagataaa
     2221 ataaatttga tctgattaga tatcggctaa gtgttattaa tttacaaaat atgaaataca
     2281 attttaaaaa gatagaaaca aaatgacaaa aagaatgagc taaaaataaa tataaaaatc
     2341 aaaaggctca ggatttttct aaaaaaagga aaatatatat tttagatatg tttccatatc
     2401 catcaggggc tggtttacat gtggggcata tagaaggata cacgggaagt gatattttat
     2461 cgagatatta tcgtttaaaa ggttttaatg ttttacatcc tatagggtat gatgcttttg
     2521 gtttgcctgc tgagcagtat gctattgcgc ataagattaa tccaacgcaa gctgtaaaag
     2581 aaaatatcaa aaattttgaa cgacaattga aaatgatggg ttttagttat gattgatcga
     2641 ggacaataaa tactactagc ccagaatatt ataaatgaac acaatgaatt tttttacaat
     2701 tgtataaaaa aggtttggca tatgaaagtg aagtacctgt aaattattgc gacaaatgta
     2761 aggctgtttt ggcagatgaa gaaacagaaa atggtaaatg ttttcgttgt gggggacatg
     2821 tgtcaagagt taatattaaa caatgaatgt taaagattac tgcatatgct gatagattgt
     2881 taagagattt aaataaagta aatttttcag aaaatgtgaa aacaatgcaa aaaaattgga
     2941 ttggtaaatc cgaaggttat gaagtaactt ttaaggtaat tggtgtacaa aaagagataa
     3001 aagtatttac tacgagatta gatactttgt ttggagcaac atatttagtt ttggcgccag
     3061 aacatccaat aataaaagat attgtatcca aagaccaaga agtaaaagta gaagaatata
     3121 taaaaaaatc tttgaataaa acagattttg aaaggcaagt agaatctaaa acaaaaacgg
     3181 gagtagccac aggtgccttt gcgataaatc caggcacaag agaacatatt cctatatggg
     3241 tttctgatta tgttttaatg agctatggta ctggggcaat tatggctgta cctgctcatg
     3301 atcaaaggga ttatgatttt gctcaaacat ttaatttaaa tattcggcaa gtaattaaac
     3361 catttgacaa tacgaataat actacaacat ctccttttgt ttatgacaaa gaaggtgttc
     3421 ttataaactc tggtcctttt actggcgtta aaagtacaat agcgagagaa aaaatagctt
     3481 tgtatgtagg tgcacagaaa aaaattaatt ataaaatgcg agattgagtt ttttctcgtc
     3541 agagatattg aggcgagcct attcctataa taaaatgtcc taaatgtggt aatgtacctt
     3601 tgagagaaaa agatttgcca ttaaaattac caaatataaa atgttatgca ccaagcaaag
     3661 gcgcagaatc accattagaa aatattagtt cctgagtaaa tgtaaaatgt cctatttgta
     3721 atagtgatgc gaaaagagaa actaatacta tgccccagtg ggcaggatca agttggtatt
     3781 ttttgagatt tttggatccc aaaaatcata aaatgtttgt tagtttgaag aaagagaaat
     3841 attgaatgcc tgttgatatt tatattggag gcacagaaca tgctgtaact catttaattt
     3901 atgcgcgatt ttgatataaa tttctttatg atttgggata tgttaattat cctgagcctt
     3961 ttgtaaaact aataaatcaa ggtatgattt tgggagcaga tcatgaaaaa atgtcaaaat
     4021 cattaggtaa tgtagtaaac ccagatgata ttgttaaaca atatggggta gatgctttac
     4081 gtttatatga aatgttttta ggggctttta acgatgccaa accttgaaat actaatagtt
     4141 ttgtaggtat agagagattt ttgaataaaa tttgaaattt atatgatgaa tattttaata
     4201 ctaagaatga aaaaccacat aaaaaagtta atactaaaaa gtttgaaatt ataataaaca
     4261 aaacaatcaa aaaagtagaa gaagatatat ctacttttgg ttttaataca gccattagcg
     4321 caatgatgga atgtttaaat gtattgactg ataacgatga agaaataaaa tttattccta
     4381 aaagtgtatt agaaaaattt attattattt tatcaccctt tgcgccattt ataactgagg
     4441 aaatttggca tgagatgttt aaaaacaaaa catctattca gaaagctgta tgaccaaaat
     4501 ataatataaa attggtggag gataaaaata ttattattgc tgttcagata aatgggaaaa
     4561 tgaaaggtac tttacaagta aaaagagata gtcgagaaca agatgtgttg acgttgttaa
     4621 acaaagataa aaagctatct ggatatgtat taggtcataa aattaaaaaa actatattta
     4681 ttccaaaccg attaataaat atattattag agtagtattt atagctaaat agtttctaaa
     4741 ggatctacat caatttttca ataatttgga accaattgta gtaattggtt tcgaattttt
     4801 aaatctttat aagtaatacc agtggggttg gttattttga gtttgataag aatatttcat
     4861 tgatattgat tttcaatttt ttgtaaaaaa gcaggagttg gaggcataat aataaaataa
     4921 tctttataga ttttatgttt ttgaataaac tgatttaaat tttggaagag ttgtttagat
     4981 tcgtgtatag cttttgtgtt atcataatgt ctataagaaa gcttaattag ttgagaatag
     5041 gggggtcata aataatgttt cttggttatt aagttttgtt taagataatc tgtgactgta
     5101 ttatttttga ttgcttgaaa taaggctatg tctctgaaca tagtttgaat aataaaatta
     5161 tttgttgcct ttacttttaa ttttaataaa tattttaata ttttttcttc ttggcgaaaa
     5221 tcaggaaaag tgaacatagc atcaatactc acaacggctg taagcgatac aggatttagt
     5281 tgcggtcgta aaattatttc agtgcccaaa agaatatcaa atttatgatt attaaaatct
     5341 tgaatgattt gacggatttt atcctcgggt atttcgctat ctattcgttt gatagtgaat
     5401 gttttgtttg tttgcgattg ttttttagat aaatgtttta gttcgtcttc tactcgttca
     5461 ataccgagtc ctatcatacg taagttgcct ccttggcaat gttcacatag agttggtgcc
     5521 aacatttctt gaccacagtg atggcaagtt aggaggttta tattgttttc tttgtgatat
     5581 atcattggca catcgcaatt actacattta aatacatatc cacaatcttc acaaacaatt
     5641 gatcttgctc agcctttacg atttaagaaa agaatagctt gctcattatt atttattgtt
     5701 tttaaaattt ctttttttaa caaagatgat aaatattgtg tttttttggg gctattaata
     5761 taagggttgt aaataaaaat attagttttg ggattagtag tggttgtatt ttcaaggtta
     5821 ttataaataa gaggtatttt tagttttttt gccattcatt ttaaaattgt ttctgtattg
     5881 taatatgggt gttgatctca agatttgtat gatgggttat ctttgtctac tataataatt
     5941 ttttctaagc gttgaaatgg ataatagggg atagatcgta gccctataac aaatttgcta
     6001 ttaccttgta aaatattaag tcaagctttg ttaaaatctt tttgatataa ttttttagga
     6061 attatagtaa tttgcgagga atcataagtt tgtaagagtg ttgatactat aaagtctaaa
     6121 tgagcttctg tggggcaagc aattaaagta gtgggtattt gattatttga ggacaagggt
     6181 ttgtcatttt tgctaagaaa tttggttaaa gattttaaat tatttggtaa taatattttg
     6241 gcagttcagg ataaattatc gaaataataa tctgaaacaa attgtaaaag ctctcaaaaa
     6301 aaaggtgata agaaatgttt attatatata actttatgta ttttttttca cttatagtct
     6361 gcatttttta gaaaagtttt tactgttttt ataggagtag aatttataac taatcctaaa
     6421 ataagcctat ggtttaggtc aatttctact atagcaccct gggtcagatc ttctgtataa
     6481 tagtatgaca taatttgtga ttcggatttt ggcagattgg ttagtggtaa aacatctaat
     6541 aagtacataa tttttattta tatagtatat tgtataaatt tttatttaat tattaaaaaa
     6601 agcttgatat agagttgtgt attgagcacc ttttctggta agttgtgatt caaataaaat
     6661 taaactatta gctacaaatt gtattggcat tattgtttct attgttgttt ttgatggtaa
     6721 taaagaaggg gtattaaaat tttgtattcg ggctaatgta atatgcggta taaatccctt
     6781 atgctctcct tgaaaattta tattaggata tgaatataat tcttcttgta atttattttt
     6841 aacatcttga agtattttat tgggtgcgcc tttaattcaa acaagtcttt gtctattcgg
     6901 agttggtcca tattcaattt tatctaatat aattgtaaag ggattaaaat gatgagcaat
     6961 tttgtttaaa atttgagaaa tttgtaaaat agcattattg tcattgtttc ctaaaaatac
     7021 caatgtaaga tgtaaattat cttttgatac tatattaaac acagaagggt ttaggtgtgt
     7081 ttgttgaaat tgtacaagtt tgttttggtt ggtgttatct aaaggtatag ctataaaaag
     7141 cctatgatta ttatacataa tatatttttg attataattt tgattataat tttagaaata
     7201 atgttataag attgtattag tgttattata tcatatttta taatataaca caataaacac
     7261 attttatagc aaaaattgta tataaattac atttttaatg aattgatttt acaaatggga
     7321 aaaatatata agacccttga attaagacgc tctttatgct atacttaata tagaaaggaa
     7381 agccgttgtg gtggaattgg cagacacgcg acactcaaaa tgtcgtggag ataactccat
     7441 gtcggttcga ctccgaccaa cggcacccaa ggaaattgta acacatagaa aattgtccat
     7501 tcaatactaa ttatttaaat taattactaa ataaatttat tttttcttaa ttcaaaattt
     7561 ttagttgata tttacttaaa atttatttaa ttgtgataag gattaactaa tattaaatat
     7621 aaaaaatgga ttattctcaa tttaataatt gaacgccaca agatggagag attgatttct
     7681 caaatttgtt atcaagtggc gagcaagaac agaataataa tattatttct tctccctcca
     7741 gtcaagattc acaaaatata ttagagatgt cagatcaaga acccaatatt gcttctttgt
     7801 cgtctgttgt aacatcaaca gagccattat ctatatcaca accaataaaa gatgataagg
     7861 ctaatctttt agttaatact tctttattga atacagatat taatataaat aataatttgg
     7921 acgtaaacga taaaagttta gaaaatacat taaatttagt aaaagatgat attatgtcca
     7981 aagatattat atcagaagta aataaccaag agcaaagtgc caatgttgat tttttgaaga
     8041 cagaaatgca tgataggatt tttcaattag aagtaaataa aataaaacca aatccatatc
     8101 aaccaagaaa agaatttgat cccaaatcaa tcgaagaatt agcggcttct ataaatgaat
     8161 atggtatttt agagccactt attgttacta ggaatgagat tgaaacagac aaaggattgg
     8221 atgtagaata ccaattaata gctggtgaga ggcgattaag ggctgcacaa tttttgggat
     8281 taaaaacagt accggcaatt ataaaaacat taaaaaacga agttgctaaa ttggagttag
     8341 ctttggtaga aaatcttcag cgagaagata ttgattctat tgctcgggca aaatcttatg
     8401 caaaattaat aaacgagttt ggttataccc aagaaatggt agctcagaaa gttggtaaaa
     8461 gtagagaaag cattgctaat acattaaggt tgttacaatt accttttgag gcgcaaagag
     8521 cgatacagga aaacaaaatt accgaaagtc atgcgcgagc tttattactt ttgcccaata
     8581 tggaaaaaca aagagcattg cttggagaaa ttttgactaa acagttaagt agtagagaag
     8641 cagatcttat agctcgtcag tttttggagc aacaggtagg gaaaaattct caatccagaa
     8701 aaagattagg aggatctttt gatccaatgg atctcgaatt gcgagaaaaa ttagaaagtg
     8761 tttttgatac aaaagttttg attaaacgaa agggacaaag aggggagatt actattcatt
     8821 ttgattccaa tgacgagctt aacgggttgg tagacaaaat gttaaaacta tctgaaaaaa
     8881 gagttaataa tacagcagga aaaattagtt tgtaatttaa atttttatat atgcgtaaca
     8941 caaaaaacac aaatgttata tttttcacca ttgttgccat tttgttgatt agtggatttg
     9001 tttttttggt tagtgcttct actgtggcag gtatggaacg aaaagttcct gatggctggt
     9061 attatgtcaa acatcaaata atgatgggtt tttgtgtggg attgcctggg ttattatttt
     9121 tttctaaatt cgattatcgt aaattaaaaa aagtatcatt agtgttattt attctgaata
     9181 ttttggcatt acttttaact tttgtaccat ttttttctgt tgctggggaa aaagcttatc
     9241 gctctttaaa tttaaaattt ataacttttc agccagccga atttttaaaa attactttaa
     9301 ttcttgtgtt agcagtatta tttactacac gttctggtaa agaacataag aatttttgga
     9361 aaacagcatt tcccttttgg acatatttag gtgtagtgag tgtaatttta ttattacagc
     9421 catccactag tatagtaata gttttggcat gtacaagtat aattatgtat tgatgtagca
     9481 atttttcaat tgtatatggt ataataggtt gtattgcttt attagttatt gtgggaataa
     9541 tttttgttgt tttatatgct agtggattgt gtactactgt gaataaaaat actgggcaaa
     9601 tacaagatag atgtttggaa tatcgttggc gaagaattca ggcttttttt aatcctcaag
     9661 atgaatcttt gtctgaaacc tatcagccaa aacaagcaaa aattggatta ggtactggag
     9721 gatggttggg tgtagggttg gggcaatcga gacaaaaata tagttattta ccagaaagtt
     9781 ttactgattc tattttcgct attattggtg aagaattagg gtttgctggt tcatttggta
     9841 taattctttt gttctttttc ttctatgttt cggggttatc aattatcaaa aatgctcctg
     9901 atgtttttgg atcttttttg gctttgggtt taattagttg aatagttgta caagctttta
     9961 ttcatatttt atcaacaagt ggttttatac cactaacagg tatacctttg ccatttatta
    10021 gttacggtgg tagtttgtat atagtaactt tgtgatcact ggg
//
LOCUS       DAOTJA010000022         9805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_7195_length_9805_cov_5.888718, whole genome shotgun sequence.
ACCESSION   DAOTJA010000022 DAOTJA010000000
VERSION     DAOTJA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_9805_cov_5.888718"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="P5241_02240"
     CDS             complement(<1..2022)
                     /locus_tag="P5241_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRV32358.1"
                     /translation="MLNDDIQREQKLKDIFDDARERNTQAKARSLGLPYLDLKKENIE
                     PVALELVDEVVARNALIVPFQKQGDIVAVGVFDPNNADTINVISQLKNQHFDVRVFVV
                     SKTSLDFAFDKYKLVPPKREQISDFINVTNFVPINFRDLNEYLAQIDSSNVTKILSLI
                     LKSAIEIDASDIHIDALEKECLIRFRIDGILFDVGKISTAVYKGIRDRIKLLASIKLN
                     VQNASQDGRFTIQNKAILFEARVSTIPGPYGEFIAIRLLNPERMSFDLQSLGLGLDNV
                     KLINSLLSTPAGMILATGPTGSGKTTTLYALLKRKISPGINIITIEDPIEYKLKGINQ
                     TQVDEEKGYDFPNGLRAIVRQDPDVIMVGEIRDQETAEMAVQSSLTGHLVFSTLHTNE
                     ASGAISRLIEMGVDRDIIPDALKLIIAQRLVRKLCPYCKEKYKPSAEIVQNIKDTLSI
                     LSPRAGIQIPNIITELYRAKGCEKCNWLGYKGQTGLFELLFVNSDIADLVRHNASIDE
                     IREKAISLGMVPLFHAGLLEVLQGNTSLEEITRVAGDIDYVKLMFAKILDQTLTRGIV
                     IDSKEISLVAKMINNLSLLETKIRDIKIADGFDLIFALALIYRASDIHIEPTDQQIVV
                     RYRIDGVLEDKLKLPKELHKLYIQHIKNLAGLNVQVTDIVQEGRFKVTEE"
     gene            complement(2172..2513)
                     /gene="tsaE"
                     /locus_tag="P5241_02245"
     CDS             complement(2172..2513)
                     /gene="tsaE"
                     /locus_tag="P5241_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRV32359.1"
                     /translation="MISKSVWQTHKLADKIAKDILNKRLYYTNNRRQAVIVLLIGSLG
                     TGKTEFTKGLARALHIKAKILSPTFLIMRKYNHFYHLDCYRLLPRPEETLKALAVDEV
                     VNNSQNIVVIE"
     gene            complement(2515..2823)
                     /locus_tag="P5241_02250"
     CDS             complement(2515..2823)
                     /locus_tag="P5241_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32360.1"
                     /translation="MGSFTPIRFTINKKIKSLGLSPFIKEVQVKAQVEAFLNNYISRG
                     DVFFVAFKNNNIYLQCSNNIIANEMRYTIEPLKDYVLQNCKSIRIHNIYIQTKNTFKS
                     "
     gene            complement(3010..4032)
                     /gene="dnaN"
                     /locus_tag="P5241_02255"
     CDS             complement(3010..4032)
                     /gene="dnaN"
                     /locus_tag="P5241_02255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRV32361.1"
                     /translation="MKFTCLIENLKKGISISEKITSQNITLPILSYILLTTDTNKLKI
                     SATDLELGLNYFIPGKVEEKGSVLIPSRIFANIINNFEGDKITLEVQDNKLTIQNDKT
                     HLVIQGGSEEEFPIIPNIDAAQFIEINSKDLLISLNQVLRSAGAGLSKPELNSVYCYQ
                     QDKDLYFVTTDTFRLSEKKILKNKYQENSINNINVIIPIKTAQELSRILQSIEESMVK
                     IFIGNNQIFFDLSSANLISRLIEGEYIQYKNIIPNSFDTEGLFNKKELQEAIKLVSLF
                     SSKVNDICLETNIQSKELIIKSQEVTIGQGIYKIAGNLTGEDLTINFNYRYLLDGLES
                     IQGDKV"
     gene            complement(4517..5743)
                     /gene="dnaA"
                     /locus_tag="P5241_02260"
                     /pseudo
     CDS             complement(4517..5743)
                     /gene="dnaA"
                     /locus_tag="P5241_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
     gene            5769..5921
                     /locus_tag="P5241_02265"
     CDS             5769..5921
                     /locus_tag="P5241_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32362.1"
                     /translation="MVKLELVPIVMVLLFLSNIAVFLNHVLKLAFDTVRAISFKIVFH
                     KSLKLS"
     gene            6266..6409
                     /gene="rpmH"
                     /locus_tag="P5241_02270"
     CDS             6266..6409
                     /gene="rpmH"
                     /locus_tag="P5241_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRV32363.1"
                     /translation="MSTKRTYQPKKIKRARTHGFLKRKSSTAGKKIINRRKNKGRYKL
                     TVS"
     gene            6423..6764
                     /gene="rnpA"
                     /locus_tag="P5241_02275"
     CDS             6423..6764
                     /gene="rnpA"
                     /locus_tag="P5241_02275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRV32364.1"
                     /translation="MLSQQYRLKGQKNFSRVLSSPNIAHGQFLSVKFIENNMGTSRFG
                     LIVSRKISPLAVKRNYIKRVISNIIMSNRQHINKGFDVVILPKKNIVNIHFSNLKNDL
                     ENTLQKAKVLY"
     gene            6774..7268
                     /locus_tag="P5241_02280"
     CDS             6774..7268
                     /locus_tag="P5241_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRV32365.1"
                     /translation="MFKLLIYQPLFNLLIIFYNLFNDLGISVILLTALIRLLLWPMFT
                     KNETNQKIMAKIQPEIKRIQKEYAKDYQKQSQELLSIYKQYKLNPSFTIIFSIAQIFI
                     ILALYNVFAIVVKPTFTYYLYQPLRATTSINYLFLNTINLQQGSLLLAIIAALTQLVQ
                     GFIM"
     gene            7532..7990
                     /locus_tag="P5241_02285"
     CDS             7532..7990
                     /locus_tag="P5241_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRV32366.1"
                     /translation="MDIEDIIKQVLTTIFQNAKVNDLQLEVTKNETEKKFVITAEITP
                     ASIFIGRGGEILDALENVIGSIIRSHTIDRWFVEIDINNYRTEKNFALKELAKKAAHR
                     VLVLKTPVRMDPMNARDRRLIHSEIALYPDLISESEGTPPNRYVVIKYRE"
     gene            complement(8081..8845)
                     /locus_tag="P5241_02290"
     CDS             complement(8081..8845)
                     /locus_tag="P5241_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRV32367.1"
                     /translation="MLLKAIFWLYIAGAIIAFISLIIAGFNYMFSGGNASKTKKAIDQ
                     IKQAFIGLFILLGAYTILYTINPDLVVIPTNLFGAPPYLEQLNVPSDMNEEAAREILI
                     EGYDYNEVEDNLVDQIKDDLRTRSDIAMDGDVIKDLDNQKLNPLILQVIDFAMNNWDK
                     PSNCGQLIVGPGVAGHVKNTDVYGPNYQSCHDLGMAIDFEIPGIDEPWGQKSDTKIEC
                     QASLQSALKKSTFYGAITILDEQTHIHIQTADCLYY"
     gene            complement(9200..>9805)
                     /locus_tag="P5241_02295"
     CDS             complement(9200..>9805)
                     /locus_tag="P5241_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32368.1"
                     /translation="CGGEYNNKIITNIRVNIINATMENITIKKIPQTTTSTEGNIKPQ
                     NISPDKQPLTQSKNIETTQQVQTKIKVQPAYKDNGSTSQYAQNDNTETQATQENDELT
                     TRAFAQGMNISWLILCVTIAIIKDIFEIIIGFIPIGDLASFILSLPLALILGLFLQLA
                     GKKNKFNSLKAAFQSIIKILMQVVDIVPIVSIIPLSTILVI"
BASE COUNT         3239 a         1456 c         1292 g         3818 t
ORIGIN      
        1 ttcttcagtg accttgaacc ttccttcttg aactatatca gtaacttgca cattcaatcc
       61 tgctaaattt ttaatatgct gaatatataa tttatgtagt tctttgggca attttaattt
      121 atcttccaac actccgtcta tacgatatct tacaactatt tgttgatctg tgggttctat
      181 atgaatatca gatgcccgat atattaaagc gagcgcaaaa attaaatcaa acccatcagc
      241 aattttaata tcacgaattt ttgtttccaa caatgacaaa ttatttatca tttttgctac
      301 taatgatatt tctttgctat caatcacaat accccgagtc aaagtttggt ctaaaatttt
      361 agcaaacatc aatttcacat aatcaatatc tcctgccacc cgagttattt cttctaaact
      421 cgtattgcct tgtaaaactt ctaacaaacc tgcatgaaac aatggtacca tacctaacga
      481 aattgctttc tcgcgtattt catcaatact agcgttatgt cgtaccaaat cagctatatc
      541 tgaattcaca aataataatt caaataatcc tgtttgccct ttataaccaa gccaattaca
      601 tttttcacaa cctttggctc tgtacaattc tgtaataatg ttcggaattt gtatcccggc
      661 ccttggtgat aatatcgata atgtatcctt tatattttgg actatttctg cagatggttt
      721 atatttttct ttacaataag ggcataattt tcttactagt ctctgggcaa taattagttt
      781 taaagcatca gggataatgt ctctatctac acccatttct atgagtctag atattgctcc
      841 agatgcttca ttagtatgta aagtagaaaa aactaaatgc cctgttaaag atgactgtac
      901 tgccatctcg gcagtttctt gatcacgaat ttctcctacc ataattacat ctgggtcttg
      961 acgcacaata gctcgcaacc catttggaaa atcataacct ttttcttcat caacctgtgt
     1021 ttgatttatc ccttttagtt tatattcgat aggatcttca atagtaataa tatttatccc
     1081 gggagatatt ttccttttta acaaagcata caaagttgta gttttccctg atcctgtagg
     1141 tccggttgcc aaaatcatac ctgctggagt agacaacaaa ctattaatca atttcacatt
     1201 atcaaggcct aaccctaaac tttgtaaatc aaacgacatc ctttcaggat ttaataatct
     1261 aatagctata aattctccat aaggccctgg gattgttgaa acacgggcct caaataatat
     1321 agccttattt tgaatggtaa atctaccatc ttgagaagca ttttgcacat ttaatttaat
     1381 gcttgctagt aatttaattc tatcccttat acctttatat acagcagtag aaattttacc
     1441 tacatcaaac aaaataccat ctatacgaaa tctaatcaag cattcttttt ctaacgcatc
     1501 aatatgtata tcagacgcat caatttctat ggcggatttt aaaataagtg ataatatttt
     1561 agttacatta gaagaatcta tctgagctaa atattcgttt aaatctctaa aattaattgg
     1621 tacaaaatta gtaacattta taaaatcaga aatttgttct cttttaggag gtactaattt
     1681 atacttatca aaagcaaaat ctaagctggt tttagatact acaaaaaccc gtacatcaaa
     1741 atgttgattt tttaattgag aaataacatt aatagtatct gcattattag gatcaaatac
     1801 ccctactgcg acaatatctc cttgcttttg aaaaggaact attaaagcat ttctagccac
     1861 gacttcgtcc actaattcta aggctactgg ctctatattt tcttttttaa gatctaaata
     1921 tggcaaaccg agtgaacgtg ctttggcttg agtattacgt tctctggcat catcaaaaat
     1981 atcttttagt ttttgttctc tttgaatatc atcatttaac ataacaaacg tttatctaat
     2041 attaattaaa cttaaacatt ttggggttta ttatacttta ctttaatatc tcgccaagtg
     2101 ctatgtttat ttattgtttt aaaataaata ttgatagtat aatattgttt taatcatttt
     2161 atattttcag ctcattctat aacaacaata ttttgggaat tattaacaac ttcatcgaca
     2221 gccaatgctt ttaatgtttc ctcggggcgc ggtaataatc tataacaatc caaatgataa
     2281 aaatgattat atttgcgcat aattagaaaa gtaggactta aaattttggc cttaatatgc
     2341 aatgcccttg ctaatccttt tgtgaattca gttttccccg tacctaacga gcctatcaat
     2401 aatacaataa cagcttgcct cctattatta gtataatata atcttttatt taaaatatct
     2461 tttgctatct tatctgctaa tttatgtgtt tgccatacac ttttgctaat catactacga
     2521 cttaaaggtg tttttagttt gaatataaat attatgtatt ctaattgatt tacaattttg
     2581 taatacataa tctttcaacg gctctatcgt atatctcatc tcattggcaa taatattatt
     2641 agaacattgt aaatatatat tattgttttt aaaagcaaca aaaaaaacat cacctcgtga
     2701 tatataatta ttaagaaaag cctctacctg cgcttttact tgtacctctt ttataaacgg
     2761 agatagccct aatgatttaa tttttttatt aattgtaaat ctgatcggcg taaaactacc
     2821 cataaaatat aaatttaata attataaatg gtgcggcaaa aattatttta aataatatat
     2881 caaatacatc tggttgaatt tgtatattta cttatactaa tactaaagtt taattggagc
     2941 agtaatatac aaataagttg tatcgtccgg atctttgaat accaaaggac gatcttggtt
     3001 taaccctatt caaaccttat ctccttgtat agattctagt ccatctaata aatatcgata
     3061 attaaaattg atagttaaat cttcacctgt aagattaccg gcaattttgt atataccttg
     3121 accaatagtc acttcttggc tttttattat aagctctttt gattggatat tcgtctctaa
     3181 acaaatatca ttaactttac tactaaacaa actaacaagt ttaatagcct cttgtaattc
     3241 ttttttatta aacaatcctt ctgtatcaaa actattagga ataatatttt tatattgaat
     3301 atactctcct tctataagcc gagaaatcaa gttggcagat gataaatcaa aaaatatttg
     3361 attattccca ataaatattt taaccataga ttcttctata ctttgtaaaa ttcttgacaa
     3421 ctcttgtgct gttttaatag gaataataac attgatatta ttaatagaat tttcttgata
     3481 tttatttttt agtatcttct tttcactcag acgaaaggtg tcggtagtta caaaatataa
     3541 atctttatct tgttgataac aatacacact atttaattct ggtttactca accctgcccc
     3601 tgcgctacgt aatacttgat tcaatgatat tagtaaatct ttactattta tttctataaa
     3661 ttgtgctgca tcaatatttg gaataattgg aaattcttct tcactacctc cttgtattac
     3721 taaatgtgtt ttgtcattct gaatagttaa tttattatct tgtacttcta atgtaatttt
     3781 atctccttca aaattattaa ttatattagc aaaaattctt gatggtatta aaactgaacc
     3841 tttttcttca actttgccag gaataaaata atttaatcca agttctaaat cagtagctga
     3901 aatttttaat ttattagtat ctgtggttaa taaaatataa gataatatag ggagggtaat
     3961 attttgacta gtaatttttt cacttataga aatacctttt tttaaatttt ctattaaaca
     4021 tgtgaatttc atattgtgag tttaatttat taaaggaaca atatattgta ttagttatat
     4081 aatttgagtt tatttattaa acgaaaatta tattgtattt gttatatatt gtgagtttaa
     4141 tttattaaag gaacaatata ttgtattagt tatataattt gagtttaatt tataatttat
     4201 taagaattaa atatttttat ataaaattta tttcccatcg acgtattata ttatatataa
     4261 attaattaat aaatttgtta ttattgttat atatgttgaa aagtgtaaat attatattat
     4321 ctcttggttt tattatattt tttcgtgttt actttagtgt aaaacattgt ataaatatgt
     4381 gtataatata tttataatta taatagtata taatatttat ttttaaatgt caattatctg
     4441 gtattatatt atattagtta taataaagtt ttccctatta taattatata gttttccact
     4501 agttatatta tctgttttat acatttgaat tcataatacg ttcagtaaga gtataaattt
     4561 cctgagccaa acctgggttg ttttttatac tctgagtaat tttatcataa gaatgcataa
     4621 ccgttgtatg gtctttattt ttaaaataag aacctatagt aggataagaa catttcataa
     4681 tattacgtaa taaatacatt gccacctgtc gtgctttaat aatatcttgt ctacgtgagc
     4741 cactttttaa atcatcttcg gctatattat aaaatgttgc cacttcttta ataataatat
     4801 taggtgataa tattttggtt ggtttttgga tataatctaa caataactgt tttacttcgt
     4861 ctaagtccgg gatttggttt ttaatttggt atcgaattaa aatagtattt aacgaacctt
     4921 ctaattcacg aatatttgtt gtgatatttt gagctatata ttctaaaata ttatttggta
     4981 aaacaatatt ttttgctttg gttttgctaa tcaaaatagc catacgtgtt tcgtaatctg
     5041 gtgcactaat atcagcaatc ataccccctt caaaacgtga gcgcaatctt tcctcaatgc
     5101 ctataatgga tttgggtggg cgatcagatg tgaaaataat ttgtttatta tgttcataca
     5161 aggcattaaa tatatggaaa atttcttctt gggttttgtc tttattggca ataaattgta
     5221 tatcgtctat caaaagacaa tcaatttgtt tgtactcttc ttttatatca gtaatagtcc
     5281 catttctaat agctttgatg attttattgg ttagggcttc tgagctagta taatatattt
     5341 ttttattttt gtagtgtttt gttattgcat tacctgtggc atgtattaaa tgtgtttttc
     5401 ctaaacccac tcccccatat ataaacaaag gattatattt tgttcctaaa ttttctatta
     5461 ctgcgcaagc agctgcatgg gcaagttcat tatatgcacc aacaataaaa ttctcaaatt
     5521 gatatttagg gtttaaattt gttttggggt ctggcgagga ttgtaataaa ttttctgttg
     5581 caatatttac ttgatttagt ttggtatgtt tgttggatga taagtttgta gtttttatag
     5641 tttgaatatt attagcgttt tgtatttggt tggtacttgt tacaacatat ttaatttcag
     5701 aaatatctgg gcatacatga tttacagcct ggacgataaa attatgatat ttttgctgga
     5761 gtcattcttt ggtaaaatta gaattagtgc caatagttat ggttttatta tttttatcta
     5821 atatagctgt atttttaaat catgtattaa aattagcatt tgatactgtg agagctattt
     5881 catttaaaat tgtatttcat aaatcattaa aattgtcatg agtcatattg tgatatttat
     5941 tctatttata gttatttatg attatgtatt attatacctg ttttttgatt tttttgcaag
     6001 tcttgttagt tgataattat tatattcttg tgcataagct gtggaaagct gtgaaaaact
     6061 aagtaaataa ctataacgac aaaatatgta aaaatttgta tttttgaaaa attatattat
     6121 atataaacaa atattttttg ttgttatata tgttgtggtg tttaatacta aaatggtaaa
     6181 tatcaaaccc cttgctatct aatttaaaat atgatagtat atttatatat attaaatgct
     6241 taattattta atttaaatat ataatatgtc tactaaaaga acatatcagc cgaaaaaaat
     6301 aaaacgggca agaacccatg gttttttgaa acgcaaatca agtactgctg gtaaaaagat
     6361 aattaacagg cgtaaaaata aaggaagata caaactaact gtttcttaat tttttattat
     6421 atatgttatc acaacaatat agattgaagg gacaaaaaaa ttttagtcga gtgttgtctt
     6481 ctcccaatat agctcatggt caatttttat cagttaaatt tattgaaaat aatatgggca
     6541 catcacgttt tggattgatt gtaagccgta aaatttcccc cttagctgtg aaacgtaatt
     6601 atattaaaag agttattagt aatattatta tgagtaatcg acaacatata aacaaaggat
     6661 tcgatgtggt tatcttacct aaaaaaaata ttgttaatat acatttttct aatttaaaaa
     6721 atgatttaga gaacacgctc caaaaagcca aagttcttta ttaaatacta tatatgttca
     6781 aacttttaat ttaccaaccc ttatttaatt tgcttattat attttataat ttatttaatg
     6841 atttaggtat tagtgtcata ttactaactg cactaatacg tttgttactc tggccgatgt
     6901 ttactaaaaa tgagactaac caaaaaataa tggccaaaat acagccagaa atcaaaagaa
     6961 ttcaaaaaga atatgccaaa gattatcaaa agcaatccca agaattatta tctatatata
     7021 aacaatataa attaaatcct agctttacta ttattttttc tatagcccaa atattcatta
     7081 ttttagcttt atataatgta tttgctattg ttgttaaacc aactttcaca tattacttat
     7141 atcaaccatt acgcgccaca acgtcaatta attatttatt tttaaacaca attaatttac
     7201 agcaagggag tttgctatta gcaattatag cagctttgac tcaattagtt caaggtttta
     7261 taatgtgacg taattctaac ccccatgatc cacaaagaaa ttctttactt attattacta
     7321 ttattatgcc cataatactt attagtttat atcaacaatt gccatccatt atatttttgt
     7381 attggatcgt tttaactatt ataggtatca ttcaaagtat atatattcaa aagcatttta
     7441 gaatgtcatt atctgtccca attgacaaaa cattagacag cccagccccc taattaatat
     7501 attaatatta ttgtttatta aatttcatac tatggatata gaagatatta ttaaacaagt
     7561 actaactact attttccaaa atgccaaagt aaatgattta caattagaag taactaaaaa
     7621 tgagactgag aaaaaatttg taattactgc tgagattacc cctgcttcta tttttattgg
     7681 tagaggtggg gaaatattag atgccttaga aaatgttatc ggatctatta ttaggtctca
     7741 tactatagat cgttggtttg tggagataga tattaataat tatcgtacag aaaaaaattt
     7801 tgctcttaaa gaattagcca aaaaagccgc ccacagagtg ttggtgttaa aaactccagt
     7861 gcggatggac cctatgaatg ctcgagatag acgattaatc cattctgaga ttgccttata
     7921 ccctgatctt attagcgaat cagaagggac tcctcctaat agatatgtgg ttataaaata
     7981 tcgagaataa attttgttgt atttactatc caaaacataa aaaagtaata ttgataatca
     8041 atattacttt ttctgtattc accaacatca atttcaaaac ttagtagtac aggcaatctg
     8101 ctgtttgaat atgtatatga gtttgctcat ctagtattgt aattgcgcca taaaatgttg
     8161 attttttgag tgcgctttgt aaagaagctt gacattcaat ttttgtatcg cttttttgtc
     8221 cccaaggttc atcaataccc ggtatttcaa aatcaattgc catacctaag tcatggcaag
     8281 attgataatt aggaccatac acatctgtgt ttttaacatg tccagccaca ccagggccga
     8341 caattaattg tccacaatta cttggcttgt cccaattatt catagcaaaa tcaataactt
     8401 gtaatattaa ggggtttaat ttttgattat ctaaatcttt gattacatct ccatccatgg
     8461 cgatatcgct acgtgttcgc agatcatctt taatttgatc taccaaatta tcttctactt
     8521 cattatagtc ataaccttct atcaaaattt ctcgggccgc ttcttcattc atatctgatg
     8581 gtacatttaa ttgctctaaa tatggtggtg ctccaaataa attagttggt ataactacta
     8641 aatcagggtt gatagtatat aaaatagtat atgctccgag caaaataaat aaaccaataa
     8701 aggcttgctt aatttggtca atggcttttt ttgttttgga agcattgcca ccactaaaca
     8761 tatagttaaa cccagctata attaaagata taaaagcaat gatagctccg gcaatatata
     8821 gccaaaaaat agcctttaat aacatattcg gtaaaccttt tacacttagt tttgtcccag
     8881 cagctactat cccaggtata cctacctcaa aaatataatc ttttggtttt tctacataat
     8941 aatctgcttt aatatttgta gtaaaaatgc ttaatataat taaagatatt aaaagaatat
     9001 aaaaattttt tgttcatata ttgtgcatca tataatttat ttaaatataa tttatttatt
     9061 aaatttttga agctattttg ctcaaaacat tttgtgcttt atttgctgcc ttgcctttac
     9121 taccagcgaa ctgcgttgcc attttaattt tacttgtggt ttgctctgtt tttttgctat
     9181 cttttttact agcaaaagat catattacta atattgtact taaaggaata atagatacta
     9241 taggtacaat atcaactact tgcataagta tttttataat actctggaaa gctgctttta
     9301 aactattaaa tttatttttt ttgcctgcta attgtaaaaa taagcccaaa attaatgcta
     9361 atggtaaact aagaataaaa cttgctagat cccctattgg tatgaaccca ataataattt
     9421 caaatatatc ttttataata gctatggtta cacatagaat aagccaagat atattcatac
     9481 cctgagcaaa ggcacgagtt gttagctcgt cattttcttg agttgcttgt gtttcggtgt
     9541 tgtcattttg ggcgtattgg gacgtagatc cattgtcttt atatgcaggt tgtactttaa
     9601 ttttagtttg tacttgttgt gttgtttcta tgtttttgga ttgtgttaga ggctgtttat
     9661 ctggcgaaat attttggggc tttatattac cttcagtaga cgtagttgtt tgaggtattt
     9721 tttttattgt tatattttcc atcgtagcat taattatatt tactcttata tttgttatta
     9781 ttttattgtt atattcccca ccgca
//
LOCUS       DAOTJA010000023         9705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_7284_length_9705_cov_5.896684, whole genome shotgun sequence.
ACCESSION   DAOTJA010000023 DAOTJA010000000
VERSION     DAOTJA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7284_length_9705_cov_5.896684"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="P5241_02300"
     CDS             <1..264
                     /locus_tag="P5241_02300"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32369.1"
                     /translation="EALDKAYNLLYSLVEEQFDPDIMKIQFEELIKYNDKYKINKGLI
                     LWPLRMALSGLKYSPSPYELLAVLGREESLRRIKIAQDRLAAV"
     gene            complement(1091..1315)
                     /locus_tag="P5241_02305"
     CDS             complement(1091..1315)
                     /locus_tag="P5241_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32370.1"
                     /translation="MKISEPEKEQISRLIKRLKNGKRLPSRDAVTNQILLRTIAQKVL
                     ENFWIMTQKEIENVMTQEEIENIKQLYKKT"
     assembly_gap    1530..1539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1650..1853
                     /locus_tag="P5241_02310"
     CDS             1650..1853
                     /locus_tag="P5241_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRV32371.1"
                     /translation="MRISHSLLETYKQCPYKYKLLVIDKLQEPKNQDAVFGSFIHLIL
                     HWFYNNHPKFMSLEELLAYYEQK"
     gene            1860..2639
                     /locus_tag="P5241_02315"
     CDS             1860..2639
                     /locus_tag="P5241_02315"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRV32372.1"
                     /translation="MRYIDFFTKKETKLDDRYFQDGKLILTNFYNTNKPNGFCDYAVL
                     DLEKRFEVVLQDKKNELHILSGIIDRIDKIDDKGYELIDYKTNKSLPSLAEVTRNPQL
                     NLYALGIKQMWPYIDISQVKFSLYFLKFNEKITINKKEEDLKKVKDSLLKDIEKIQKT
                     KEFNPKPSPLCNWCGFKDRCPVFKEKYQNNDINNIKNQEEIAKLIDQYFDLEDQKRGL
                     ERQIKGLKSEIDAYCQANNISRVYGKQGYFTRESLDEETYD"
     gene            complement(3169..3246)
                     /locus_tag="P5241_02320"
                     /pseudo
     CDS             complement(3169..3246)
                     /locus_tag="P5241_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
     gene            complement(3325..3903)
                     /locus_tag="P5241_02325"
     CDS             complement(3325..3903)
                     /locus_tag="P5241_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRV32373.1"
                     /translation="MDEKKILIVNLSKGLTGEENSALLGALIITKIQLAAMSRADVST
                     ETRDPFYLYIDEFQTFSTESFANILSEARKYKLNLILAHQYINQLDDTVKDAVFGNVG
                     NLISFRIGIEDALFLEKEFEPEIHAQDLINLPSYYFYIKMMIDNMPARPFMARELGPL
                     APSKETFRTEIIDNCHNLYSTPRENVEEYIKK"
     gene            complement(4204..4599)
                     /locus_tag="P5241_02330"
     CDS             complement(4204..4599)
                     /locus_tag="P5241_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRV32374.1"
                     /translation="MANNNNKEITLLGTTNFRGNPVQFGITQQDRLYHCYIIGKAGSG
                     KSTLMENMCYSDIIKGRGVGLIDPHGEFAEKLLDYIPRDRIKDVVYINPSDIDWPVAF
                     NIIEEVSPEMRHIVKSGLMSVFKKIWPDV"
     gene            complement(4629..4934)
                     /locus_tag="P5241_02335"
     CDS             complement(4629..4934)
                     /locus_tag="P5241_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32375.1"
                     /translation="MFLKQFNNPCGNSIEEIKNDAPLVKMKTKIRNKIFPYIPTQISR
                     QMFQKAINRKPSEKEIIISSKELASIFHFPFQNIYLSSVVMKKNRPGSPPPNLPYIP"
     gene            5074..5220
                     /locus_tag="P5241_02340"
     CDS             5074..5220
                     /locus_tag="P5241_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32376.1"
                     /translation="MGVDTVVVVGAEVITEVMALLSESPIDFVNIRPTDSGFCGAALP
                     IKGL"
     gene            complement(5481..5852)
                     /locus_tag="P5241_02345"
     CDS             complement(5481..5852)
                     /locus_tag="P5241_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32377.1"
                     /translation="MPLILFNIFRDLLKLYLKELRKKESNFICLQLLFSEEACLKPLR
                     FMELFFDNLKNIKLEEDQNISCEIVGIDNQLKYFIRMPEKAQDLIAIILHSQYPNVEL
                     VQVNDYFSRIPPNVPNNEFSF"
     gene            complement(5968..6219)
                     /locus_tag="P5241_02350"
     CDS             complement(5968..6219)
                     /locus_tag="P5241_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32378.1"
                     /translation="MQASNSPLSEYEISQVAHKQRIVKDSTIHLSLSDKKLFQKTQDN
                     KYILSKSVLQKQNTDLTSTPTPKPTQNTQDKEEYKILNA"
     gene            complement(6262..6666)
                     /locus_tag="P5241_02355"
     CDS             complement(6262..6666)
                     /locus_tag="P5241_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32379.1"
                     /translation="MSHEQKPLTTQQILERLAPNNKNLNSKILENILSINNKIYKNPI
                     GEYGLISWSNIRPYSAKDKAYFLMKYYIKKPIHFRDLAKLIQSYEFPTLVKESNSIAT
                     KKKNINIQTVHNELIKDNRFTLIGHGQYALQE"
     gene            complement(6727..7137)
                     /locus_tag="P5241_02360"
     CDS             complement(6727..7137)
                     /locus_tag="P5241_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRV32380.1"
                     /translation="MTQNNTLSNLTKSILKNLTGRNKNIIEKRFGLFSDKKYTLQDIG
                     NELHITRERIRQIEKESIKTLKQFKPTKEFNKFISEVKKILIANNGFCEENKFFAILS
                     KKYNDKNLDKFTIFLTTLNGSIIHVEANNCYKAY"
     gene            complement(7362..7925)
                     /gene="efp"
                     /locus_tag="P5241_02365"
     CDS             complement(7362..7925)
                     /gene="efp"
                     /locus_tag="P5241_02365"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRV32381.1"
                     /translation="MVSASDFTKGMIIEYENQPCQILEYTFNKPGKGGGFMKTKLRNL
                     KTGSIFEINFRTQDTFKELEPEHRQVIFIYYNNKEIFFNDKETNKRFSLPISLIGNKA
                     KFLKTNMDLDISYINDEPININIPIKITYKVVSAPPAIKGNTSSGATKIVTLENNLTL
                     STPLFIKEGDYIVVNTEKGEYVERSND"
     gene            complement(8143..8215)
                     /locus_tag="P5241_02370"
     tRNA            complement(8143..8215)
                     /locus_tag="P5241_02370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8180..8182),aa:Lys,seq:ttt)
     gene            complement(8470..8574)
                     /gene="rrf"
                     /locus_tag="P5241_02375"
     rRNA            complement(8470..8574)
                     /gene="rrf"
                     /locus_tag="P5241_02375"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8702..>9705)
                     /locus_tag="P5241_02380"
     rRNA            complement(8702..>9705)
                     /locus_tag="P5241_02380"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3212 a         1305 c         1596 g         3582 t
ORIGIN      
        1 gaagctcttg acaaagcata taatttatta tatagtttag ttgaggaaca atttgatcca
       61 gatattatga aaattcagtt tgaagagcta attaaatata atgataaata caaaattaat
      121 aaaggtctta ttttatggcc gttaaggatg gctttatcag ggcttaaata ttcaccaagc
      181 ccatacgaac ttttagctgt tttagggcgt gaagaatcgc ttagaaggat aaagatagcc
      241 caagaccgtc tcgcggcagt ttaaaagcca tttaaacgcg ttttatgaac aatttaaata
      301 cctttttaga caaagtagca gagatattca cccagatatc gcagtctttg tatgggctta
      361 taaataagat atttgaagag caaatgttaa gccatttgtg acaaattgcc aaaggttttg
      421 gccacatagt aataggagta ttggaagcat tgttgggcgt tctgaaatct attattaaat
      481 aaatgataaa aatataggta gtaggatata gtagtaggcg gtattattac aggatattaa
      541 aaataaaaga aaatgaatag tatgtcgtaa aaggtatatt atgcgacgta tgatataaaa
      601 gaaaatatag gataactccc aagttccctc ttatattaat acttatattt ttattagttc
      661 ttaagcatat tatttatgta aatatacttt tgatatttat atatttttta tagcaatttt
      721 attttatttt gttatttaga tatatataag attgagattt gtttttattt ttcttttgtt
      781 ttgtttcttt gatatttgag ttctgggtat aatatagaaa aaatataaat atcaaggagc
      841 ctgtgtaaaa cctgtgtaaa aaaagcttga caaaaaaaat attatatgat agtattatag
      901 gaccattagg ataaataatt tattttaccg aaagataaaa taaattgtct cgcaagagat
      961 agttctaatg tacaaagatg ctccgcaagg agcgtttttg tttttttata aaaaatcata
     1021 taatttatat caattagata taaatataaa ttacgccact acataatatt gtatagtggc
     1081 gttgttttgt ttatgtcttt ttgtataatt gtttaatgtt ttctatttct tcctgggtca
     1141 taacgttttc tatttctttc tgggtcataa tccagaaatt ttctaatacc ttttgcgcaa
     1201 tagtgcgcaa aaggatttga ttcgttacag cgtctcggct tggtaaccgc tttccatttt
     1261 tcagccgctt tattaatcgg ctgatttgtt ctttttctgg ttccgatatt ttcatttttt
     1321 tatctccttt cttgttttta aattgctatt atcaatatag catatttttt aatatttgtc
     1381 aatagacatg ttttcgtttg tcgtttgtcg ttttgacttt ctaatatgat aatgtttgag
     1441 tatatcaaaa caagaatgag atgatgttta tcttatatca tttaaatacc taatatttag
     1501 atacttagta tatgaatata taaaattaan nnnnnnnnna aatcgcagaa ttaatatcaa
     1561 acaaaaaatc taatgaatat ataaaattaa aagtaaagcc ttgataaaaa acaaattttg
     1621 tggtaatatt taattattaa acaatattta tgagaatttc acattcattg ttagagacat
     1681 ataaacaatg tccgtataaa tataaattat tagtaattga taaattacaa gagcccaaaa
     1741 atcaagacgc tgtttttggt tcttttattc atttaatttt acactggttt tataataacc
     1801 atcctaagtt tatgtcttta gaggaattgt tagcttatta tgaacaaaaa tgagatgact
     1861 tgaggtatat cgattttttt actaaaaaag aaacaaaatt agatgataga tattttcaag
     1921 atgggaaatt aatactgact aatttttata atactaataa gccaaatggt ttttgtgatt
     1981 atgcagtttt ggatttagaa aaaagatttg aggttgtatt acaagataaa aaaaatgaat
     2041 tacatatttt gtcaggaatt attgatagga tagataaaat tgatgataaa ggttacgagc
     2101 ttattgatta caagactaat aaatcactcc cctctttggc agaagtaaca agaaacccgc
     2161 aattaaattt gtatgcttta ggaataaaac aaatgtggcc atatattgat ataagtcaag
     2221 taaagtttag tttgtatttt ttgaaattta acgaaaaaat tacaattaac aaaaaagaag
     2281 aagatctaaa aaaagtcaag gatagtttgt taaaagacat agaaaaaatt caaaaaacta
     2341 aagaatttaa ccctaagcca tctcctttat gtaattggtg tggatttaaa gatagatgcc
     2401 cagtttttaa agaaaagtat caaaataatg acataaataa tattaaaaac caggaagaaa
     2461 tagccaaatt aatagatcag tattttgact tggaagatca aaaaagaggg ctagaaaggc
     2521 aaattaaggg ccttaaaagc gaaattgatg cttattgtca ggctaataat ataagtaggg
     2581 tttatggaaa acaggggtat tttaccagag aatccttgga cgaagaaact tatgattgaa
     2641 ttaaagtaag agaactgtta cgacctctta aaaaatggaa taagttatta actgtacaaa
     2701 aagaaaaatt agatgaggta ttaaaagaaa tacctgaaga tattagaata gaaattgaac
     2761 gcactaaaac tactaagaaa acaacaaaaa ttattcaggc tactcactat aaagatttat
     2821 ctccttttca aaatggtgat gtaaaagata ataaagaggg gttaaaattg gaagcagaag
     2881 attcattatt ggaagcctaa aataaccttg tgttttttat ttattaaata aattttttaa
     2941 caggtgtttg agccctgttg tatcaggaga tggtttggat ttagtatgga ttgatttatt
     3001 ttctttgttt gattctttgg ttatattttt tgattctttg gttatatttt gagaattaga
     3061 tatattttgt tgattagata acaagatatt gttatttgta ttaaaattag aattattgtt
     3121 ttgtagagta gatattgatg gttgagtatt ttgttgttgg gttttaagtc aggtaaaaca
     3181 tttctcgcaa taaattgggc gtgtggggtc tggttgaaac ggaactaatg tttttgtacc
     3241 acatcttgag caagttgctt gtcaatattc tgtttgacta cgaggagttg gtttattgtt
     3301 agatggaaca aattccaggg cgcttcattt ttttatatat tcttctacat tttctctagg
     3361 tgtgctatat agattatgac agttatcaat aatttctgta cgaaatgtct ctttacttgg
     3421 agccaatgga cctaattctc ttgccataaa tggtcttgct ggcatattat ctatcatcat
     3481 ttttatataa aaataataac ttggaagatt aattaaatct tgagcatgaa tttctggctc
     3541 aaattctttt tctaaaaata aagcatcttc aataccaata cgaaaagaaa ttaggttgcc
     3601 aacattacca aatacggcat ctttgactgt atcatctaat tgatttatat attgatgagc
     3661 taaaattaag tttagtttgt attttctggc ttctgataat atattagcaa aagattcggt
     3721 agaaaatgtt tggaattcat caatatataa ataaaaagga tctcgtgttt ctgtagatac
     3781 atctgctcga gacatagctg ccagttgaat tttggtaata attaaagctc caagtaaagc
     3841 actattttcc tctcctgtta gccctttaga caaatttaca atcaaaattt ttttctcatc
     3901 cataattttt cgcatatcaa ttttggtatc gacttgtcca ataatatttc tgatcaacgg
     3961 atttgaaata aattgtccta ctttgttttg aataggagca atagcttcgg tttgaaattt
     4021 ttcgttatac ttatcaaatt cactatatca aaaagatttt actacagcat cagtgacatg
     4081 atttaatact gttttgcgat aatgtttatc agaaagcata cggttaatag aaagtaaagt
     4141 agcacccgga tattctaata atgctaagat tgtattgctt agaatgtatt ccatacgtgc
     4201 agatcaaaca tctggccata tttttttgaa tactgacatt agtcctgatt ttacaatatg
     4261 gcgcatttct ggactgactt cttcaataat attaaaagct actggccaat caatatcaga
     4321 tgggtttata tagacaacat cttttattcg gtctcgagga atataatcta ataatttttc
     4381 tgcaaactcg ccatggggat caatcaaacc aaccccccgc cctttgataa tatcagaata
     4441 gcacatattc tccattaaag tggatttacc tgatccagct ttgccaatta tataacagtg
     4501 ataaagacga tcttgttgag tgatgccaaa ttggactggg ttaccacgaa aattagtagt
     4561 gcccaaaagc gttatttctt tgttattatt gtttgccata aaaaatttta attaagatga
     4621 ttttttaatt aaggaatata tggtaagttg ggaggaggag atcctggacg attttttttc
     4681 attactacag aagatagata tatattttga aatggaaaat gaaatataga agctaattct
     4741 ttagaactta taataatttc tttttcagat ggttttctat tgatagcttt ttgaaacatt
     4801 tgacgagata tttgagtggg gatataaggg aaaattttgt tgcgtatttt tgttttcatt
     4861 ttaactaagg gcgcatcatt tttgatttct tctatagaat tgccacaagg attattgaat
     4921 tgtttgagaa acatttctaa acttgtagta acagaattgg aaaaatcagt tgggcgacta
     4981 atataattta aacgaatact tgatttatag ttagtaccgc tgattttttt ttcaatattt
     5041 ttgatagttg cttgttcggt atctgtggga gttttgggtg tggatactgt tgtagttgtc
     5101 ggggcagaag ttattacaga ggtaatggca ctattgagtg agtcccctat tgattttgtt
     5161 aatatacgtc caacagattc tggtttttgc ggtgctgctt tacctattaa aggattataa
     5221 attgattggg cttcaaaatc tcaatctgtt ttttctttgt cgtttaaatt acgaataata
     5281 cattgtacag aaataatatc gtctggacga atgcgattaa aaatttctat tattactgat
     5341 aatggatcgt aagaattagg ctcaataata ttgtctgtta tattactact ttttagtttc
     5401 tcttgtgttt taatgttgta aaaattctca taagttaaaa gaggaaatgg attacctttt
     5461 ttatatttat attctttgct tcaaaaacta aactcattgt taggaacatt tggtggtata
     5521 cgtgaaaaat aatcattaac ttgtactaat tcaacattag ggtattgaga atgcaaaata
     5581 attgctatta gatcttgtgc tttttcaggc atgcgaataa aatattttaa ctgattgtct
     5641 attccaacaa tttcacaaga tatattttga tcttcctcta atttaatatt ttttaggtta
     5701 tcaaaaaaaa gctccataaa ccgcaagggc ttaagacaag cttcttcaga aaataaaagc
     5761 tgtaaacaaa taaaattaga ttcttttttt cttaattctt ttaaatataa ttttaataag
     5821 tcacgaaaaa tattaaataa aattagcggc aagatgattc atcaaaactc tcgaaatata
     5881 cctattatta ttctccaaat agtcataaag attgtgtaaa attgagacat aatttggctg
     5941 tttaatattg tcaaatgtgc ttaaacatta ggcatttaaa attttatatt cttctttgtc
     6001 ttgtgtgttt tgagtgggtt taggtgttgg tgtagaagtg agatcagtat tttgtttttg
     6061 aagtacagat ttagatagaa tatatttgtt gtcttgggtt ttttggaata gttttttatc
     6121 acttaaactt agatgaatag tgctatcttt tactattctt tgtttatggg cgacttgact
     6181 aatttcgtat tctgataaag ggctgttgga tgcttgcaaa atattaataa taacatcttt
     6241 tatagctcct ttggtataac ctcattcttg tagagcatat tgaccatgac caattagagt
     6301 aaatctgtta tctttgatta gttcattatg aactgtttga atatttatat tttttttctt
     6361 ggtagcaata gaattagatt cttttactaa tgtagggaat tcgtatgatt gtattagttt
     6421 ggcgagatct ctaaaatgaa taggtttttt gatgtagtat ttcattaaga aatatgcctt
     6481 gtctttggcg ctataaggac gaatatttga ccaagaaatt aaaccatatt caccgattgg
     6541 atttttataa attttattgt tgatagataa aatattttct aggattttgc tatttaagtt
     6601 tttattatta ggtgcaaggc gttcaagtat ttgttgggta gttaaaggtt tttgttcgtg
     6661 ggacaatagg tcagtgacat tttgtagtac atttttaatt aaagctgttt tttctttatt
     6721 tattgctcag taagctttat aacaattatt tgcttcgaca tgaataatag aaccatttaa
     6781 tgttgtgaga aaaatagtaa atttatcgag atttttatca ttatattttt tgctcaaaat
     6841 agcaaaaaat ttgttttctt cgcagaaacc attattagct atcaagattt ttttgacttc
     6901 agatataaat ttattgaatt ctttggttgg cttgaattgt tttagagttt ttatactctc
     6961 tttttcgatt tgcctgattc tctctcgggt aatatgtagc tcattcccta tatcctgtaa
     7021 tgtatatttt ttatcagaga atagtccaaa gcgtttttca ataatatttt tatttcgtcc
     7081 agtaagattt ttgagtattg actttgtaag attagaaagt gtattatttt gtgtcatagt
     7141 aaaagtttaa tataatgatt gcctataata cttgctgatg tatggttata ttgtttatat
     7201 aatatagtta tcaatcaatt agtttcaata tggtgataga ttttggggat atactggatt
     7261 gtcaagacgc attttgggat gtattataag ctctattata agtataccaa actattgcct
     7321 ttatgtcaat acctaagatc gtaggttatt atatggttat attagtcatt agaacgttcg
     7381 acatattcac ctttttcggt atttacaaca atataatccc cttctttgat aaacaaagga
     7441 gtgcttaatg ttaaattatt ttctaatgtg actatttttg tagctccaga agatgtattg
     7501 ccttttatag ctggtggtgc agaaactact ttataggtta ttttaatagg tatatttata
     7561 tttattggct cgtcattaat ataagaaata tcaagatcca tattggtttt taaaaattta
     7621 gctttattac ctatcaaaga tattggtagg ctaaatcttt tatttgtttc cttatcgtta
     7681 aaaaatattt ctttattgtt ataataaata aaaataactt gacgatgttc tggttctaat
     7741 tctttaaaag tatcttgggt tctgaaattt atttcaaaaa tagaaccagt ttttaaattt
     7801 cgtaatttgg ttttcataaa acctccgccc tttccaggtt tattaaaagt atattctaaa
     7861 atttgacaag gttgattttc atattcaata atcatacctt ttgtgaaatc tgatgctgaa
     7921 accataaaat aattatttaa tatgagattg ttaataaata aataaattgg gtgtaagatc
     7981 ctttctaatt tttaataaat gttgtttaaa aaatgtagat gtatgtttaa aatacagagt
     8041 ttaaaataca gacagtaaaa agtaatttat atttctgtcc aactactaat tacaaactat
     8101 ttaaaactga attttaatga aataaaaatt tttagttttt agtgccccct acaggattcg
     8161 gacctgcgac catcagctta aaaggctgct gctctaccga ctgagctaaa ggggcaaata
     8221 ctaaataaaa tattacaaaa tattgtattg attttttgtt atttgttttg agcaggcctt
     8281 cgtagacctt tgaaatcgct ctctaacgcc tcttttccac gtttttaaga gttaagtttt
     8341 tatattttat aatattacta taaaattaat caaaagtcaa ggttttatat agagaattaa
     8401 agattctggg cgcaagaagg tttaaaaagt gcaagaaggt ttaaaagaaa taattttttt
     8461 attaaaaatg cccggcaaac cctaccttcc ctaaacagta ttatcgggtc atagcagttt
     8521 cacttccgtg ttcggaatgg gaacgggtgg tgccagctat gataaatcac cgggcaaaaa
     8581 ttaacaattg ttaaaaataa atcaaaacag ttaaaataaa ttagtaaaac aaattatttg
     8641 acaattaaaa attataaatg ataaaaattt agattttaca taacagaaaa gaataaatta
     8701 agatgaggaa acttcgattt attagtactt cttggctaaa aatattacta ttcttacacc
     8761 taaagcctat caacccaata atcttttggg aatctataac gatgtctaat cttgaggaag
     8821 gtttcccact tagatgcttt cagcggttat ccttatccaa acttagctac ccagcaatgc
     8881 tcttggtaga acaactggta caccagaggt tcagtcatcc aaatcctctc gtactatgga
     8941 cgagtcctct caaacatcga acgccaatgg tagatagaag tccatactgc cttacgacgt
     9001 attgaaccca gctcgtgtat ccttttaacc ggcgaacagc cggacccttg ggacctgctt
     9061 cagccccggg ataggataag ccgacatcga ggtgccgagc gaagccgtcg atatgaactt
     9121 tcgggcttca ctagcctgtt atccctggag tagcttttgt ccgaagtctt caccctttct
     9181 ataaagaggt gtcgggtcac taaactctgc tttcgcaata gcgcggaatg tcttccttgc
     9241 tataaagcta gcttatgctt ttacactatc tcctgaattt ccatctcagg atagctaacc
     9301 ttaataggct cctccgttac tttttaggag gatcgcgccc cacgaaaact gccaaccaaa
     9361 caatgtccct gtatagtttt tctatacggg ttagatttta agctttgaaa gatgggtgtt
     9421 tcattgtcgg atcagcttta accgagatta aagcctcaaa tcctccccac tacgctacgc
     9481 attcaaatcc taaaatcaat gtcagattac agtaaagctt ccagggtctt cttgtctaac
     9541 cactggtacc cagcgtcttc actggactcg cattttcacc gagcatttcg ttgagacagt
     9601 tgcttagtcg ttataccatt catgcgcgtc ggaacttacc cgacaaggaa ttacgctacc
     9661 ttagaacgat tatagttatc gctgtcattg accagggctt agagc
//
LOCUS       DAOTJA010000024         9511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_7432_length_9511_cov_6.054357, whole genome shotgun sequence.
ACCESSION   DAOTJA010000024 DAOTJA010000000
VERSION     DAOTJA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7432_length_9511_cov_6.054357"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="P5241_02385"
     CDS             <1..291
                     /locus_tag="P5241_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV32382.1"
                     /translation="TIFLTTQYINEAEICDRVAIIDYGEIVACDTPDNLKKMISTDLV
                     VLKTANNQEAIVELKTNFPDLNIKQLGKNILIEAENGETLLPQIIEKTSVKI"
     gene            542..697
                     /locus_tag="P5241_02390"
     CDS             542..697
                     /locus_tag="P5241_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32383.1"
                     /translation="MPFILVLIFGQGISSMLPSDIIGFDFAHFFYSGVLVLSVVVAVF
                     DSTISLV"
     gene            713..1036
                     /locus_tag="P5241_02395"
                     /pseudo
     CDS             713..1036
                     /locus_tag="P5241_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1037..1297
                     /locus_tag="P5241_02400"
     CDS             1037..1297
                     /locus_tag="P5241_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32384.1"
                     /translation="MQIISHYNPLFYALNGLRYILGGKFIPPEYTHLIFYNFGFSLFI
                     VFAFALVCFVGAILVFNKISILSMLVKIIEENEENNTQNTHS"
     gene            1520..1822
                     /locus_tag="P5241_02405"
     CDS             1520..1822
                     /locus_tag="P5241_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32385.1"
                     /translation="MIYSNIYKYKKTQLVFGITLAVMFALLFIQASPAKALTVADYDG
                     IPSACPDKYPYNTKDCATFIKPINVNGYSDNDTSLSARTTPVSYGYIIPGKTITVS"
     assembly_gap    2379..2478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2479..2711
                     /locus_tag="P5241_02410"
     CDS             <2479..2711
                     /locus_tag="P5241_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32386.1"
                     /translation="QNGAYSTLAECQSHCQAPTPQYGYVCNTATYQCTYIQNGAYSTL
                     AECQSHCQAPAPTVTISANPSTIDYGQTSILT"
     gene            3114..3362
                     /locus_tag="P5241_02415"
     CDS             3114..3362
                     /locus_tag="P5241_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32387.1"
                     /translation="MILTVTEPSIGTILTLNKIGRNLTMGERIYSKVVRVNTGDTVEF
                     YLQINNVSNTVATNVTILDKLPSELSYVSGSTKINNAV"
     gene            3402..3635
                     /locus_tag="P5241_02420"
     CDS             3402..3635
                     /locus_tag="P5241_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32388.1"
                     /translation="MQPNEGKTITFQATIRTPSNLFAITNVAELRADNMNVITDSTTV
                     TYSAVAGAATVNTGPGRTMVIILGSTSSITASI"
     gene            3977..4048
                     /locus_tag="P5241_02425"
     tRNA            3977..4048
                     /locus_tag="P5241_02425"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4009..4011,aa:His,seq:gtg)
     gene            4191..4493
                     /locus_tag="P5241_02430"
     CDS             4191..4493
                     /locus_tag="P5241_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32389.1"
                     /translation="MKKFIWSIIVLLVLVLIYDGAIALYNYFVPQVSTLPDIDMSPIP
                     IQDKQIQEETDTYTINIIYPETNQDFINQDINDFINKEIANFKQAITLQTNPVEAN"
     gene            4752..4874
                     /locus_tag="P5241_02435"
     CDS             4752..4874
                     /locus_tag="P5241_02435"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRV32390.1"
                     /translation="MAPKIENYQTFNITPDSCIFYFQEAQIAPYSAGPQQIEIK"
     gene            4894..5853
                     /locus_tag="P5241_02440"
                     /pseudo
     CDS             4894..5853
                     /locus_tag="P5241_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
     gene            6060..6413
                     /locus_tag="P5241_02445"
     CDS             6060..6413
                     /locus_tag="P5241_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRV32391.1"
                     /translation="MLYIGADHRGFYLKEKIKEFLKTANIDFQDIGAFKYNKDDDYVD
                     FASQVAKEVQKNPKQNKGIIICGTGIGNVIVANKFKNIRATQALTALMAQRSREHNDA
                     NILSLAADFTDPSIA"
     gene            7125..7520
                     /locus_tag="P5241_02450"
     CDS             7125..7520
                     /locus_tag="P5241_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32392.1"
                     /translation="MITHIEAIKDFNFLYNTTKKAGVELGLAISPETSTKQLYPYINK
                     TSFVLILGVVPGPSGQEFETYVLNKIKELKRKYPKVKIEADGGINLENAKTICSAGAD
                     YLAVGSYIFDAPKPLEALELLKQSLQTQC"
     gene            7581..8555
                     /locus_tag="P5241_02455"
     CDS             7581..8555
                     /locus_tag="P5241_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRV32393.1"
                     /translation="MTKNHLPKVVVIGGGTGVFTVLNALKDYPVHLQAIVSTADDGGS
                     SGVLREEFGILPPGDIRRALIALAPDSMQSLSRLFNYRFTKGEGLRGHTFGNLFLTAL
                     ERLNGDFNTAIKEAGKILNIKGEVIPVTLENVKLLAKLQNGTFIIGETNIDRPKHKIS
                     KIQEIFLSPRPKANKEALNAIKKANYIIVGPGDLYTSIIPNFLPIGLATAFKKSKAKK
                     FFICNITTKFGETDGFGAINFLKTMEKYVGKNTFNYFIVNKSRDLPKQFIKKYLKENK
                     EIVRYSDKDFINYPKLKIIQADLVRHTGLLRHDPEKLGKLLYGLILTD"
     gene            8787..9392
                     /locus_tag="P5241_02460"
     CDS             8787..9392
                     /locus_tag="P5241_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32394.1"
                     /translation="MSSKEQPDVAKMKKQFDEKPKSEQQEILKEDQLKAISVDEQLEK
                     KKKQSAFNVLMSKLDALINPDNYKEEKYIFSDKDIQQNTQRIEGIPIILAESIKLFLS
                     YVEESNFVMGVHHNLYQEDCIGNQEINKKDIDYIAHRFQTQFKTKLVDLKDGSKRIDI
                     VPEGSKIKTQNDITIDRLSIVFQKSHSDKDKYFVRLECVKG"
BASE COUNT         3737 a         1287 c         1436 g         2951 t
ORIGIN      
        1 acaatatttt tgacaactca atatataaat gaagctgaaa tttgtgacag agtggctatt
       61 attgattatg gagaaatagt tgcttgcgat actcctgata atttaaaaaa gatgatcagc
      121 acagatttgg tagtattaaa aactgctaat aatcaagaag ctattgtgga gttaaaaaca
      181 aattttccag atttgaatat taaacaatta ggcaaaaata ttttaataga agcagaaaac
      241 ggagagacat tactgccaca aattattgaa aaaacttctg taaaaatatg atctataaat
      301 ctacaaaaac ccactttgga agatatattt ttggagttaa ctggacgaca aatcagagac
      361 aaaaatccag aaaatggtaa attaagtatt cacaatcatt tataataaaa tataacaact
      421 aaacaagtat aatatgttta ataaatatgt cataaaagaa tggcaagcta taaatattat
      481 ttggcagcga gaattgcttt tgttttgaag aaacaaatta aaggtattaa cttctatttt
      541 tttgcctttt attttggttc ttatttttgg tcaaggaatt tcatcaatgc tcccatcaga
      601 tataataggt tttgattttg ctcatttctt ttattctgga gtattggtct tgtcagtggt
      661 agtagcagta tttgattcta ctatatcttt ggtatgagac agggaatttg gatttatgcg
      721 agaaatactt gtggcaccta tttcaaaaac agaaatagct attggtaaaa tgttgggagc
      781 aattagtaga ggattaatcc aaggcatact tattttaatg gtagcaccat ttttacatat
      841 ttctctttct gtttctacta ttggactttg tttattattt attattttaa tatcaacagg
      901 tatggctggc ttgggtataa tggtatcaag ccaaatatcg agaatggaaa ctttttcttt
      961 gataatgcaa tttattgttg ctccattggt atttttgtca ggagcatttt tcccaattga
     1021 aaaaattcct cagtgaatgc aaataattag tcactataat cctttgtttt atgctttgaa
     1081 tggattgcgt tatattttag gaggtaaatt tattccccca gaatatactc atttgatttt
     1141 ctacaatttt ggcttttcat tatttattgt gttcgccttt gcacttgttt gttttgtagg
     1201 ggcaattttg gtatttaata aaatatctat tttatcaatg cttgtaaaaa taatcgaaga
     1261 aaacgaagaa aataacaccc aaaatacaca ctcctaagag gtcttgacaa ataataaaaa
     1321 atatgatata attaccttgt cttaaacaac atagacataa ttaatatttt gatcctgttt
     1381 taacttattt tatatcgtgg tataattttc gtgtgcttga attgaaaaat atactaaaaa
     1441 acactttatt tattaattat ttaatggttt tttaagtcgt tttgatctta aattttaaaa
     1501 aaaataataa ttttatgtta tgatatattc aaatatttat aaatacaaaa aaacacaatt
     1561 agtttttggg ataactcttg cggttatgtt tgctttgtta tttattcagg catcaccggc
     1621 caaagctttg actgtggctg actatgatgg tataccaagt gcttgtccag ataaatatcc
     1681 atataatact aaagattgtg ctacttttat taagccaatt aatgttaatg gatatagtga
     1741 caatgatact agccttagtg ctagaacaac tcctgtaagt tatggatata taattcctgg
     1801 taaaactatt actgttagtt gaagcaaatc aggtacatct ccatccagct atttagtaaa
     1861 gtttggatgc ggtaattcta ttgattttac caatgacagc tatacaatag gatgaactac
     1921 aaactcaagt attcaatgaa atgttccaag ctgagtttct gattacaaat attgtaaagt
     1981 ttgagtttat gcaaaacaaa atagctcagg ctcgagtaat aatccaacat tgggtgtaag
     2041 taataccaga ccttttattg tacaaaaacc tgaaactcca gccttacaag tctcatgtga
     2101 tgcttccccg aatccgggag aaataggtga taaaataacc tttagtgttt atgctagtgg
     2161 tggttctggt gcatatacct attcttgatc tggtgacgta tctggaacta cacaaaaagt
     2221 ttataaaagt ttttcgacct caggcattaa aactgcttat gtaattgtaa aagatacaga
     2281 tggtaaagaa caaaaaacat cgtgctatgt tactattaaa gaacaggcac ctacaccaca
     2341 atatggttat gtttgtaata ctgctacata tcaatgtann nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnta caaaatggag catatagtac attagcagaa tgtcaaagtc
     2521 attgtcaggc acctacacca caatatggtt atgtttgtaa tactgctaca tatcaatgta
     2581 catacataca aaatggagca tatagtacat tagcagaatg tcaaagtcat tgtcaggcac
     2641 cagctccaac agtaactatt agtgcaaatc catcaacaat tgattatgga caaacttcga
     2701 ttttaacttg aagctctaca aacgcaattt cttgtactgc ttcaggagct tgaagtggtg
     2761 gcaaaaatac ttatggcagc gaagtagtaa accccaacag cacatcttct tacataatta
     2821 cttgtactaa ttcaagtgga gtatctgctt cggcaacaac tactgtaaat gtaagatatg
     2881 ctaatcctta cgttgaacta aatgtatatc catcaagtat agtaaaagga caaacagcta
     2941 ctttatcgtg atcaggatca aatctttcaa cttgtacagc tactagtacc tgaagtggta
     3001 gtgcatttag tggatataaa aatacgagtg gctatgaacc aacttcgcct gataccacca
     3061 ccacttatac aatcaattgt attgggacaa atggtggata tgtttctgat tctgtgattt
     3121 taacagtaac tgaacccagc ataggtacta ttttgacatt aaataaaatt ggacgtaatc
     3181 tcactatggg tgaaagaata tatagcaaag ttgttagagt aaatactgga gatactgtag
     3241 aattttattt acaaataaac aatgtatcta ataccgtagc cactaatgtt acaattcttg
     3301 ataaattacc tagcgaactt tcttatgtgt ctggttctac aaaaattaat aacgcagtat
     3361 gacaagatgg cattgcaact acaggtatta atgtaggaac aatgcagcca aatgaaggta
     3421 aaactattac ttttcaagcg acaatcagaa caccttctaa tttattcgct ataactaatg
     3481 ttgccgaatt aagagctgac aatatgaatg ttattacaga ctccacaact gttacttata
     3541 gtgcagttgc tggtgcagct actgtcaata ctggacctgg acgtacaatg gtaataattt
     3601 taggcagtac atcaagtatc acagcaagta tctgatattt cttaaataaa acacaaaaag
     3661 gtaaaaactt ttgagaaaaa tatcagaaaa ataatattaa aaagcgaata aacaaaataa
     3721 gagttaaata gataacaaga gatacataag taaagcataa tatagttatc ataataaata
     3781 tctaaaaaac gccctcattt aatttgaggg cgtttttatg tgatgcaata tgtaaaagtt
     3841 atatgatatt aaaatagata ttcttgttta ttcttttttg gtatattaaa atattatcat
     3901 actaagttga cattttgtat taattgttta caaaaaaaca aaaatatgat aatataatta
     3961 tattgctgtt tatatggcgg ctgtagctca gcggtagagc gcctgtctgt ggcacaggat
     4021 gtcgtgggtt caaatcccat cagccgccca attattcaca aataacttga catattcccc
     4081 aatttgtttt ggtatggttt tgatttgcaa aaaaattata aaagtgctat aatttataat
     4141 atagatagct aacaatcaaa tagttttaag aaaaaattaa aataaaaaat atgaaaaaat
     4201 ttatatggtc aataatagtt ctattagtat tagtactaat atatgatggg gctattgctt
     4261 tatacaatta ttttgtgcca caagtatcaa cattaccgga tattgatatg tcaccaatac
     4321 ccatccaaga caaacaaatt caagaggaaa ctgatactta tactattaat attatatatc
     4381 cggaaactaa tcaagatttt ataaatcagg atatcaatga tttcattaac aaagaaattg
     4441 ctaatttcaa acaagcaatt actttacaaa ctaatcctgt agaagcaaac tgacaatata
     4501 cattgtttat tagatacgat acagtatata atgaaaggac aattgtaagc tttaaatttg
     4561 aaatagaaca attcttgggt ggtgctcatc ctacacattt aattgttgca aaaaattata
     4621 attgattctt aaaacgaatc tatactattg atacggctat tcctaaccaa gaaacattag
     4681 ataaaatagc caaggtggct ttagattatt ttcagaatat gccaaaagat tgagaattat
     4741 tcccagaagg attggcacca aaaatagaaa attatcaaac atttaatata acaccagatt
     4801 cgtgtatatt ttattttcag gaagcccaaa tagctcctta ctctgctggt cctcaacaaa
     4861 ttgaaataaa atgaagtgaa atacaataaa aatatgacaa aaatatttat aacgcgaaaa
     4921 attattgatt cagctcttag tcctttattc aaaaataatt ataatgttgt cataggagga
     4981 gaaggaccgt atttagatag agcaactcta aaagaaaaat caactaatag cgacgcgatt
     5041 attacaatcg tgggagacaa tatagacaaa gaaataatcg acttgtcacc aaatctaaaa
     5101 attattgcta ctgcttctgt tgggtttgat aatattgata tagaatatgc caaatcaaaa
     5161 aatatttttg tcagtaatac acctgcggcc aatgctttat gtgtggcaga acatacaatg
     5221 gcttttattt taaatatctg ccacagaata actgaaagcg accaatttgt tagagatggg
     5281 aaatacaaag gttttgattt tcagctaatg ctcggcgatg aaataagtaa taatatatta
     5341 gggattgttg gattgggcgc tattggtaaa ttggtagcac aaatggcaat aaatggtttt
     5401 aatatgaaag taaaatatta tgatgtcact cgacaagaaa attgagaaaa agaatataat
     5461 atagaatttg aacctgattt tgataattta ttaaagactt gcgatattct atcactacat
     5521 gtaccactaa atgatcatac aaaacatatg ataaatgctg aaaaattagc cttaatgaaa
     5581 ccaacgtctt acttaattaa cacttgcaga ggtgctgtag tagacgaaca agcattaata
     5641 gaaacattaa aaaatcataa tattaaagga gcttgtttag atgtatacga acacgagcca
     5701 aacctgagtc ctggattaac tgatttacaa aatgtaattt tgacacctca tacagcagct
     5761 gcgtctttgg aagcaagaac caaaatgtca gaaatggcag taagaaatgt tttacaagca
     5821 ctatctggac aaacaccaga aaatttaata taataacaaa taagagattg ggagaaataa
     5881 caaataagaa ataactcggg ggttccgtat caaataacag gagaattatc aaaaaataca
     5941 aataaatata ataacaaata agaacaaata attaaattat taaactaaca agggtcagaa
     6001 ataaaaaaat agtaacttat aaactatttg ctataaacta aaacctataa actaattata
     6061 tgttatatat tggtgctgat cataggggtt tttacctaaa agaaaaaata aaagaatttt
     6121 taaaaacagc caatattgat tttcaagata ttggtgcttt taaatataat aaagacgatg
     6181 attatgtcga ctttgcttcg caagttgcca aagaagtaca aaaaaatcca aaacaaaaca
     6241 aaggtattat tatttgtgga acagggattg gcaatgttat tgtggcaaat aagtttaaaa
     6301 atattagagc tactcaagcc ttaactgccc ttatggccca aagatcccgc gagcataatg
     6361 acgccaatat attatcatta gctgctgatt ttactgatcc aagcatcgcc tgaaacatca
     6421 taaaaagatt tctaaaaaca tcatttatac ctaaaccaaa ctacatacgt agaataaaaa
     6481 aaatacaaaa aatagagaaa aatataaaaa aataatctcg gggcataata ttaaatataa
     6541 atagaaatat ccaatatata caaataataa aataataata aataaaataa caaacaaaca
     6601 aaaaagaaat aacctagggg ggtaaataga aatataaaac ctgtgacaaa taaaagataa
     6661 aaatagcaag cgaaatataa acagaaagag caaatatata tacaaataat aaaaatataa
     6721 taaataaaaa acaaaataac aaataagaaa tagtaaatgg aataacaaat aagaaataga
     6781 atacgaaaca cgagaataaa acatagcagg ctaaatacaa agatacgcat caaatatata
     6841 tataaataat aaaaatataa taaataaaaa ttaatatgat tgaaattatc cccgtgttaa
     6901 atgtcaaaac ctttccaaaa ttacaaaaac accttagttt attaaaaaaa tataagggtt
     6961 tacttcaatt ggatgtcgca gatggcaaat ttacaacttg aaaaaatttt catgatattg
     7021 ccaaaattaa acttttgaaa ataaaaaatc caatagaaat tcatctaatg attaatgatc
     7081 ctgaaacata tattcaagat tgactatttt taaaaccaaa aagaataatt actcatatag
     7141 aagcaataaa agattttaac tttttatata atacaacaaa aaaagctggc gtagaattag
     7201 gattggctat aagtcccgaa acatcaacaa aacaattata tccatatata aacaagacat
     7261 cttttgtgtt aatattgggg gttgtgcctg gaccttcggg gcaagaattt gaaacatatg
     7321 ttttaaataa aataaaagaa ttaaaaagaa aatacccaaa ggtaaaaata gaagcagatg
     7381 gaggaataaa tttagaaaat gccaaaacaa tatgtagtgc tggcgctgat tatttagcag
     7441 ttggttcata tatttttgat gcaccaaaac cattagaagc cttggaatta ttaaaacaaa
     7501 gcctacaaac tcaatgctaa ctcaatgcta aaagatacca attgttattt atgttgtaat
     7561 atttaatttt tatttgaatt atgaccaaaa atcatttacc aaaagtagtt gttataggtg
     7621 gaggaacagg agtatttaca gttttgaatg ctttaaaaga ctatcctgtg catttacaag
     7681 caattgtaag tacagctgat gacggtggtt cttctggggt attaagagag gaatttggta
     7741 ttttgccacc tggcgatatt agaagagctt taatagccct tgcacctgat tcaatgcaaa
     7801 gtctatcacg actttttaat tatcgtttta ctaagggaga aggattgcga ggacatacgt
     7861 ttggtaattt atttttaaca gctttggaac gattaaatgg cgattttaat acagcaataa
     7921 aagaagcagg aaaaatttta aatataaagg gggaagtaat cccagtaact ttagagaatg
     7981 taaaattatt agctaaatta caaaatggca cattcataat aggagaaact aatatagata
     8041 gaccaaaaca taaaattagt aaaattcaag aaatttttct aagcccaaga ccaaaagcaa
     8101 ataaagaagc attaaatgct atcaaaaaag ccaattatat tattgttggt cccggggatt
     8161 tatatacaag tattattcct aactttttac ctattggttt agcaacggct ttcaaaaaaa
     8221 gcaaggcaaa aaaatttttt atctgtaaca tcaccaccaa gtttggtgaa acagatggtt
     8281 ttggagcaat aaattttctc aagacaatgg aaaaatatgt aggaaaaaat acatttaatt
     8341 actttatagt aaataaatct cgagatttac ccaaacaatt tattaaaaaa tatttaaaag
     8401 aaaacaagga aatagtaaga tatagcgata aagattttat aaattacccc aagctcaaaa
     8461 ttattcaagc tgatttagtt agacacactg ggctattaag gcacgaccca gaaaaattag
     8521 gaaaattatt atacggtctc atactgacag attaataaat ttagacaata agatcaatat
     8581 agatatataa ctagtatatt cagacattta ttctgaatat actagtttta ttatcaatta
     8641 tcagaggtta tttacaaaaa gataaaaatt tgatagtata gttatagcat acaaggaatg
     8701 aatacataag gaaccaataa taaataaaaa aataataatc ggtcgacaag tatatataaa
     8761 tttaataaaa tattaaactt tttaatatga gtagcaaaga acaacctgat gtggctaaga
     8821 tgaaaaaaca atttgatgaa aaacccaaat ctgaacaaca agaaatatta aaggaagatc
     8881 aattaaaagc tatatcagta gatgagcaat tagaaaaaaa gaaaaaacaa tctgctttca
     8941 atgtgttaat gtctaaatta gatgcattaa taaatccaga taattataaa gaagaaaaat
     9001 atattttttc tgataaagat attcaacaaa acacacaaag gatagaaggc attcctatta
     9061 tattagcaga aagcattaaa ctatttcttt cctatgtaga agaatctaat tttgttatgg
     9121 gcgttcatca taatttatat caagaagatt gcattggcaa tcaagaaata aataaaaaag
     9181 atattgatta tattgcccat cgatttcaaa ctcaatttaa aacaaagtta gttgatttaa
     9241 aagatggatc taaaagaatt gatattgttc cagaaggatc taaaattaaa acacaaaatg
     9301 atattacaat tgatagatta tcaatagtat ttcaaaaatc ccattcagat aaggataaat
     9361 atttcgtacg cctagaatgt gtaaaggggt agtttctata attgatttct caaatatact
     9421 ttttagattt tgctgtatat tattggtttt gtgtttataa tcaaaagatt ttagttctat
     9481 gttttttatt atattataat attgaacttt a
//
LOCUS       DAOTJA010000025         9294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_7607_length_9294_cov_9.698236, whole genome shotgun sequence.
ACCESSION   DAOTJA010000025 DAOTJA010000000
VERSION     DAOTJA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_9294_cov_9.698236"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5241_02465"
     CDS             <1..410
                     /locus_tag="P5241_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32395.1"
                     /translation="AKMVGPEKGYVYFQDFIPNNTFDIRIIIVANKAFAIKRLTRKND
                     FRASGSGMIIYNKEEIDEQCVKIAFIVNEKIKSQSIAFDFVFDADNNPMIVEISYGFI
                     KEVYDPCEGFWNKDLNWHEGPFDPYGWMVELVK"
     gene            1115..1942
                     /locus_tag="P5241_02470"
     CDS             1115..1942
                     /locus_tag="P5241_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRV32396.1"
                     /translation="MSKSLLFIPLIFSPLIIYEIIYRPNWGGIYNAGVFRIFGTNVWP
                     NTYALHIDIIIIFTLSMYFGTKKYLYLFLSLLYAIFLFHTYSRTGLLALVIGVVVIIL
                     LRGYCFKLNIANILSIVMIILMFFFFITKFPSSRLSNINRYALLGERVNIWNDIMPQM
                     SGHYFTGRGLGGYEEIRGDVERGLSAHNAYLYILSNIGVIGLIFYLIILSYLLINIYK
                     IMINSNEINVKIFSRGTIANIILMSIFGFVGEAPLMLQATFIFWIILGFIYRYKGYV"
     gene            1935..3041
                     /locus_tag="P5241_02475"
     CDS             1935..3041
                     /locus_tag="P5241_02475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32397.1"
                     /translation="MYKIKILHVIENLRSGGKERRLVELIKGLKNYQDIQCELVIMSN
                     DIHYQYIFMQDISVHYLIRNWRKDPRIFFKLYNLCKEFKPDIIHSWGSMPSVYAFPIA
                     KLLRIKFINAMISDAPLRLKVFSKAWIRSKLTFPFSDVVLSNSYAGLKSYNAPKKKSY
                     CIHNGFDFKRLKKIGDKETVKSKFGIETDKVVGMVASFSEKKDYKTFILSAQYILPKR
                     KDLTFVAVGDGINLEKFKNIVSQEFQNRIKFLEKQKDIESIINIFDIGVLSTYTEGIS
                     NSILEYMALGKPAVATEGGGTKELIIDGETGFLVEQKNYIQLADKIEYLLENEKVAVE
                     MGEKGRKRIKKEFGLEKMTNSYVSLYRRLLNQGI"
     gene            3041..3316
                     /locus_tag="P5241_02480"
     CDS             3041..3316
                     /locus_tag="P5241_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32398.1"
                     /translation="MMKILGIGCADNYLKNISRELQVEHKVIFTGFIPEEKIYDYYFC
                     DVFVSADIADYDITVHTAIGFNKNIVSTADYEFEPELLKSVKFPCLI"
     gene            3516..4637
                     /locus_tag="P5241_02485"
     CDS             3516..4637
                     /locus_tag="P5241_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32399.1"
                     /translation="MEKKYFLTVIQSTHASLSGLESSGLLDRHKYLLNEYSKVFNVIL
                     YSSDDKNYSKKLKVTHKIPKWLPNKFGFRHFFYYIWLIIKAPKMQGIIKVFGSNIPTL
                     PIVKLLSHQKMIVTYQWDYAKQTKKNESKGLKSWMPPLLEKLSLISADIVFVTTEWLK
                     EKIDKVYNKKTVLIPNWVDFDKLEKIGFDNINKGKIILYAGRLHWSKGVDILIDAYSK
                     IYKDNHDLKLIICGDGEERKKLEEKVKPNRLKGITFTGRISNDDVLNYMTNARIFVLP
                     TLTMEGHPKALIEAMAAKCICLASRVPGNQEVLIEAGCEENLFEPGDVDSLCSKIIWA
                     MNNDNSAKTYSYAKENYSADVLIKKDIEVLKEIVGVYNE"
     gene            4630..5142
                     /locus_tag="P5241_02490"
     CDS             4630..5142
                     /locus_tag="P5241_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRV32400.1"
                     /translation="MNDLYNINKILTRFLPKDDGSKRKKLAARYARGSILDVGCSQSP
                     NNFLVGKEIIGLDVVPPSKIKFRNYTDFILGGCTKIEQIFKGRKFDTVIALELIEHLP
                     NYIVFFNNVFSVLNKDGIFIVSTPNPLLWRTVIVNALLPRGYSYSGRGIDGKLTKIHI
                     MVILFCISQE"
     gene            5265..6368
                     /gene="glf"
                     /locus_tag="P5241_02495"
     CDS             5265..6368
                     /gene="glf"
                     /locus_tag="P5241_02495"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HRV32401.1"
                     /translation="MYDYLIVGAGLFGSVCSRELSNAGKECLIIDKRKHIGGNCYTEN
                     IEGINVHKYGAHIFHTSDENIWKYINKYSGFINYIHRVKVKYKNRIYSFPINLLTLYQ
                     LWGVCDPEEAKNKIKQKQITIKNPHNLEDWILSQVGEEIYNIFIKGYTEKQWQRSPSE
                     LPTSIIRRLPIRFTYNDDYFNDKFQGIPEDGYTKIFNNLLKYIPVKLNTNFLDNREYF
                     LNIAKKIIYTGKIDEYFNYVYGRLEYRSLLFKHEILDIDDYQGISVINYPDLKIPFTR
                     IIEHKHFEGNKDHIKKTIITREYPQLYDYTNDPYYPINDDKNNKLFDKYRRLAKKYPN
                     LIFGGRLGEYKYYDMDEVISSALRCTERELKHE"
     gene            6361..7278
                     /locus_tag="P5241_02500"
     CDS             6361..7278
                     /locus_tag="P5241_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32402.1"
                     /translation="MNELLVDIIFICFNRKDEVLYNIFKMGEYKLINKVICVDNHSSD
                     GTDEIFKSLNNKNIKYIKLGKNVGIQAYNIAASNSNADILIILDDDSHIDEDAIPKVL
                     NKFNNDSRLGILAFCVKLSKTGENITKDWKDGAATSFWGCGAAVRREIWNRLGGYNKD
                     LFLYTNEYELSIRCWNIGYKVIYDSSIIAYHRVSNMNRTTKRLIIYSIRNNTYFIKTY
                     FDKKYHIKLLLIDRINWFIRSIMCNSVKSFLYGLVEARRFTNCIINNPVDHDIQKFYL
                     YNSRLFELPLKKLKRKIKDGTIFNKQNLI"
     gene            7280..8398
                     /locus_tag="P5241_02505"
     CDS             7280..8398
                     /locus_tag="P5241_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV32403.1"
                     /translation="MIKIAFIGIKGLPSLDGASRVVENIIFNLDKNKYKITVYSNGKY
                     YPKRDHFSDFTKITIPYLPVSNNINTFLYFLNSALHSLFFGDYDLVHLHNIDCAFIIP
                     ILKLKYKVICTSHGSDFSELGKWGPLGNMYFKIMNWIFFKYSEYITTVSPIEARKYNT
                     INSRIVCYIPNGVNLEEKPNIFDIDKILEQFDLKNEYILFSAGRIIPVKGLHILLNAL
                     IKINYKDKCAIIGDLNQTKEYRQTILKLIHETGSVYLGFIRDKSKLLGVILKSSIFVF
                     PSLLENMSMMLLEVASIKTPIICSDIPANTAIFSDDEVLFFKSGDVQDLAEKLKWAIS
                     NPDEMKKKSLRAYNKVNKKYLWSNITKQYEKLYQQILR"
     gene            8744..>9294
                     /locus_tag="P5241_02510"
     CDS             8744..>9294
                     /locus_tag="P5241_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32404.1"
                     /translation="MPHIDVINIPEFKIAQEWAIFIPYSLLWIVIFQYLGLYKRHIIF
                     TKSKQTVLITKGIIIGFFILILAQFIIRPSVMLDSRLMLIYFLLLCTVLLISYRLLLI
                     RPIFRSKWLGNLLSENVLIIGAGKRGKLLASKIMEEQEFQSEVIGFLDDNIERGMIVF
                     QDFKILGKLDDIKKVIKDYRVNSV"
BASE COUNT         3482 a         1120 c         1478 g         3214 t
ORIGIN      
        1 tggcaaagat ggtaggtccc gaaaagggat atgtatattt tcaggatttc atacccaata
       61 atacatttga catcaggata ataattgtag ctaataaagc gttcgccatt aaacggttaa
      121 cacgtaaaaa cgattttaga gcttccggaa gtggaatgat tatatataac aaagaagaaa
      181 tagatgaaca atgcgtgaaa atcgctttca tagtgaatga gaaaataaaa agccaaagca
      241 ttgcattcga tttcgttttc gatgctgaca ataacccgat gatcgtagaa attagttatg
      301 ggtttataaa agaagtatat gacccttgtg agggtttttg gaataaagat ttaaattggc
      361 atgaaggtcc attcgatccc tatggctgga tggttgagtt agtaaaataa atacacacta
      421 aatcggacaa actacattga aaaaaacgga cacatatcta aaatataccc ttttcatact
      481 gtttatattt tatgaatcgc aagggatatt ctatgaaagc ggatccatta ttttccgttc
      541 aaccttagct gttattattg cgattagtgg tatatatatg gtaaaaacat tcgctttgcc
      601 aagaaaaaag gattttataa tttaagattt tataaattcg ttaaaaaaag attaaatgaa
      661 aaactcaggt ttattatatt taatagtaat tccgttattt tttattgtaa attggatcat
      721 taagcatttt tcattatttt acgatttcag ttcgcaaata tttttaatat taaatatatt
      781 tataatatta ttaatattaa aacatgatgt taggctattt ataatatttg ttgcccttac
      841 tattaatttt tcatatagga tacagttttc ttacttatcg ataacctatt tgggatttat
      901 atatatatat tttataatat tatttttata ttacttattt ttaaaaaaag gaataaatat
      961 aaaagaggta agatacttat tattaataat tgcttatgct ataattatat ctggtgatat
     1021 caatattaga agttatcttt catatatttt taatttattt ggaggattta tagtattaat
     1081 catttcttat tttgttaaag ataataattt tagattgtca aaatcattat tatttatacc
     1141 attaatattt tctccactaa taatatatga aattatttat agaccaaatt ggggtgggat
     1201 ttataacgca ggagttttta ggatttttgg tactaatgtt tggccaaata cttatgcgtt
     1261 acatatagat attataatta tttttacatt atctatgtat tttgggacaa aaaaatattt
     1321 atatttattc ctatctctat tgtatgcaat atttttattt catacatact ctcgtactgg
     1381 tttattagct ttagtaattg gtgttgttgt aataatctta ttgagaggtt attgtttcaa
     1441 attaaatatt gcaaatattt tatcgatagt tatgattatt ctaatgtttt tcttttttat
     1501 tacaaaattc ccttcttcta gactaagtaa tattaatcgt tatgctttac ttggagaaag
     1561 agtaaatatt tggaatgata tcatgccaca aatgtctgga cattatttta ctggtagagg
     1621 acttggtgga tatgaagaaa taaggggaga tgttgaaagg ggtttaagcg ctcataatgc
     1681 atatttatat atactttcaa atattggcgt tattggtttg attttttatt taattatatt
     1741 gtcgtattta ttaataaata tttataaaat aatgatcaat agcaacgaaa tcaatgtaaa
     1801 aatattttcc aggggcacta tcgctaatat tatattaatg tccatatttg gatttgttgg
     1861 agaggcgcca cttatgctac aagctacttt tattttttgg attatcttag gttttattta
     1921 tcgatataaa ggctatgtat aaaataaaaa tattacatgt tattgagaat cttagatctg
     1981 gtggcaaaga gcgtcgttta gttgaattaa taaagggttt aaaaaattat caagacattc
     2041 agtgtgagct tgtaattatg tctaatgata ttcattatca gtatatattt atgcaagata
     2101 taagtgttca ttatctaatc cgaaactgga ggaaagaccc gagaatattt tttaaactat
     2161 ataacctttg taaagagttt aaaccagata ttattcacag ttggggttca atgccctctg
     2221 tatatgcttt tcctattgca aagttattga ggataaagtt tataaatgca atgatatctg
     2281 atgcaccttt gagattgaaa gttttcagca aagcctggat acggtcaaaa ctcactttcc
     2341 cattttcaga tgttgtgcta tctaattctt atgccggttt gaaatcctat aatgccccaa
     2401 agaagaagag ttattgtatc cataatggtt ttgatttcaa aaggcttaaa aaaattggcg
     2461 acaaagaaac tgtaaaatca aaatttggca tagaaaccga taaagtcgtg ggaatggtgg
     2521 cttctttttc tgagaaaaaa gactataaga catttatctt gtcagcacaa tatatcttac
     2581 caaaaagaaa ggatttgact tttgtagctg tcggtgacgg aataaattta gaaaaattta
     2641 aaaatattgt gtcgcaagaa tttcaaaaca gaataaaatt tctcgagaaa caaaaagata
     2701 tagaatcaat aattaatatt tttgatattg gagttctatc gacttatact gaaggaatat
     2761 ctaactcaat attggaatat atggctttag gtaaaccggc agttgctaca gaaggcggcg
     2821 ggacaaaaga attgataatt gatggagaga caggtttttt ggtagaacaa aaaaattata
     2881 ttcaattggc tgataagatt gaatacttac ttgaaaatga aaaagttgct gttgagatgg
     2941 gagaaaaagg gagaaaaaga attaaaaaag aatttggttt agaaaaaatg acaaactctt
     3001 atgtaagtct gtacagaagg ctgctaaacc aagggattta atgatgaaaa ttttaggaat
     3061 aggttgtgct gataattatc taaaaaatat ttccagagaa cttcaagtag aacataaagt
     3121 tatttttaca ggatttatac cagaagaaaa aatttatgat tattatttct gcgatgtttt
     3181 tgtttctgca gatatagctg attatgatat tacagtacat acagctatag gttttaataa
     3241 aaatatagta tctactgccg attatgaatt tgaaccagaa ttattaaaat ctgtaaaatt
     3301 tccttgtctc atataattga gaatgatatt gcagaaaata ttaaaaaagt gttaaaaagc
     3361 gaaactttag cgctaaagta taattgttct aatttttcct gggaatatta ttttaataat
     3421 atttacgtta tttaaatttc aaccaagtaa taggaaatat ttaagattat tttaaagata
     3481 ttaaactata gcgataatca tatcttgata aaattgtgga aaagaaatat tttttaactg
     3541 ttattcaatc aactcatgcc tctctaagtg gattagagtc ctccggatta ttagataggc
     3601 ataaatattt acttaatgaa tacagtaaag tatttaacgt cattttatac agttctgatg
     3661 ataaaaatta ctccaaaaaa cttaaagtta cccataaaat accaaaatgg cttcctaata
     3721 aattcggatt tcgacatttt ttctattata tctggcttat aataaaagca cctaaaatgc
     3781 aaggtattat taaagtcttt ggtagcaata tacctacttt gcctatagta aaacttttat
     3841 ctcatcaaaa aatgattgtt acatatcaat gggactatgc caagcaaaca aaaaagaatg
     3901 aaagcaaagg actaaaaagc tggatgccac ctttattaga aaaattatct ttaatatccg
     3961 cagatattgt ctttgttact actgaatggc taaaagaaaa gatagataaa gtttataata
     4021 aaaaaacagt acttatacct aattgggtgg attttgataa attagaaaaa ataggatttg
     4081 ataatataaa taagggtaaa ataatactat atgcaggaag attgcactgg agtaaaggtg
     4141 tagatatatt aattgatgct tattctaaaa tatataaaga taatcatgat ttaaaattaa
     4201 ttatatgcgg tgacggtgaa gaaaggaaaa aacttgaaga aaaagttaaa cctaatcgtt
     4261 taaaaggtat tacttttacg ggtaggatct ctaatgatga tgttttaaat tatatgacta
     4321 acgcaagaat ttttgtgctt cctacattaa caatggaagg gcatccaaaa gctttaatag
     4381 aagcaatggc ggcaaaatgt atttgtcttg cttctcgagt acctgggaat caagaggtat
     4441 taatcgaagc cgggtgtgaa gaaaacttat ttgaaccagg tgatgttgat tccttatgtt
     4501 caaaaataat atgggctatg aataatgata attcagctaa gacttattct tatgcaaaag
     4561 aaaattactc tgcggatgtt ttgattaaaa aggatattga agtattaaaa gaaattgtcg
     4621 gggtttataa tgaatgacct atacaacatt aataaaattc tcaccagatt tttaccaaaa
     4681 gatgatggat caaaaagaaa aaagctagca gccagatatg ctcgcggatc tattcttgat
     4741 gtaggttgct ctcaatcacc aaataacttc ttggtaggaa aagagataat cggacttgat
     4801 gttgtccccc cctcaaaaat aaaatttaga aattatactg attttatact tggcggttgt
     4861 actaaaattg aacaaatatt caagggaaga aaatttgata ctgttattgc tttagaactt
     4921 atagagcatt taccaaacta catagttttt tttaataatg tattttctgt tttgaataaa
     4981 gatggaattt ttatagtttc tacaccaaat cctctattat ggcgtactgt tattgttaat
     5041 gcattattac ctcgaggata ttcttattct ggacgaggta ttgatggaaa attaacaaaa
     5101 atccatatta tggtcatatt attttgcatc agccaagaat aattaatgca gttgctaatg
     5161 aaataggatt taaactttta aaaatattgt catcgggtag aggtttaaat tttccttttt
     5221 tacaaagaaa tttgatgtac atatataaaa aggattgaat gaatatgtat gattatttaa
     5281 ttgtaggagc tggtcttttt ggatcagtat gttcgagaga attatcaaat gcaggaaagg
     5341 aatgtctcat aatagataaa agaaaacata ttggaggtaa ttgctataca gaaaatatcg
     5401 aaggtattaa tgtccataaa tatggagcac atatatttca tacttcagat gaaaatattt
     5461 ggaaatatat aaataaatat tctggtttta ttaattatat acatagagta aaagtaaaat
     5521 acaaaaacag aatatattct tttccaataa acttacttac tttgtatcaa ttatggggtg
     5581 tgtgtgaccc agaggaagcg aaaaataaaa taaagcaaaa acaaattact attaaaaatc
     5641 ctcataattt ggaggactgg attttatcac aagtaggtga agaaatatat aatattttca
     5701 ttaaaggata tacggaaaag caatggcagc gatctccgtc agaattacct acttctatta
     5761 taagaagact gccaattaga tttacttata atgacgatta ttttaatgat aaatttcaag
     5821 gaatacctga ggatggttat acaaaaatat ttaataattt attaaaatac atacccgtta
     5881 aactaaatac taatttttta gataatagag aatatttttt aaatattgct aaaaagataa
     5941 tttataccgg taaaattgat gaatacttta attatgtata tggtagatta gaatataggt
     6001 ctttattatt taaacatgag attttagata tagatgatta tcagggtatt tctgttatta
     6061 actatccaga tttgaagatt ccatttacaa gaattattga gcataaacat tttgaaggta
     6121 ataaagatca tattaagaaa acaattatta ctagagaata tcctcagtta tatgattata
     6181 ctaatgaccc ttactaccca ataaatgacg ataaaaacaa taaactgttt gataaatata
     6241 gacgattagc taaaaaatac ccgaatctga tttttggggg gcgattagga gagtataaat
     6301 attatgatat ggatgaagta attagttcag cattaaggtg tacggaaaga gaattaaaac
     6361 atgaatgaat tattggtaga tataatattt atatgtttca atagaaaaga tgaagtattg
     6421 tataatatat ttaaaatggg agagtacaaa cttatcaata aagtaatttg tgtagataat
     6481 cattcatctg atggaacaga tgaaatattt aaatcattaa ataacaaaaa catcaaatac
     6541 ataaaattgg gtaaaaatgt tggtattcaa gcgtacaata tagctgccag caattctaat
     6601 gcagatattt taataatact tgatgacgac tctcatatag atgaagatgc aataccgaaa
     6661 gtattaaata agtttaataa tgattccaga ttaggaattt tagcattctg tgtaaagtta
     6721 tcaaaaactg gagaaaatat cacaaaagac tggaaagatg gcgccgccac ttctttttgg
     6781 ggatgtgggg cagcagtaag aagagaaata tggaatagac tggggggata taacaaagat
     6841 ttatttttat atactaatga atatgagtta tctataaggt gttggaatat tgggtataaa
     6901 gtaatatatg attcttcaat cattgcatat catagagttt cgaatatgaa taggacaaca
     6961 aaaagactca ttatttattc aataagaaat aatacgtatt ttattaaaac atattttgat
     7021 aagaaatatc atattaaact attacttatt gatagaataa actggtttat acggtctatt
     7081 atgtgtaatt cggtaaaatc ttttttgtac ggtttagtag aagcgagaag atttaccaat
     7141 tgtataataa ataatcctgt tgatcatgat attcaaaagt tttacttata taattcgaga
     7201 ttgtttgaac taccactcaa aaagttgaaa agaaaaatta aagatggcac tatatttaat
     7261 aaacaaaatt taatttaaaa tgataaaaat tgcattcatt ggtataaaag gattaccatc
     7321 actagatggt gcatcaagag tcgtagaaaa catcatattc aatttagata aaaataagta
     7381 taaaataact gtttattcaa atggaaaata ttacccgaaa agagatcact tcagtgactt
     7441 tactaagatt actattcctt atttaccggt ttccaacaat ataaatacgt tcctatactt
     7501 tttaaattct gccttacatt cactattttt tggcgattat gatttagtcc atttacataa
     7561 catagattgt gcttttatta ttcctatttt aaaactaaaa tataaagtaa tctgcacatc
     7621 tcacggatct gatttttcag aactaggcaa atggggaccg ttaggaaata tgtattttaa
     7681 aataatgaat tggatatttt ttaaatattc ggaatatatt actactgttt caccgattga
     7741 agccagaaaa tataatacaa ttaactcaag aatagtttgt tatataccta atggagttaa
     7801 tcttgaagaa aaaccaaata ttttcgatat tgataaaatt ttagaacaat ttgatttaaa
     7861 aaatgaatac atattgtttt cagcgggaag aattatacct gtaaagggtt tacatatatt
     7921 gttaaatgcg ttaataaaaa taaattacaa agataaatgt gcaataattg gagatctaaa
     7981 tcaaacaaaa gaatatagac aaacaatatt aaaactaatc catgaaactg gttctgtata
     8041 cttaggcttc ataagggaca aatcaaaatt attaggagtt atattaaaat cttcaatttt
     8101 tgtctttcct tcgttattag agaatatgtc aatgatgctt ttagaagttg caagtataaa
     8161 aacccctatt atatgcagtg acattcccgc aaatactgcg atatttagtg atgatgaagt
     8221 tttatttttt aaatctggtg atgtacaaga cttagcagaa aaattgaaat gggctatatc
     8281 aaatcctgat gaaatgaaaa aaaaatcttt gagagcatat aataaagtta ataaaaagta
     8341 tttatggtca aatataacaa aacaatatga gaaactttac caacaaatat taagatgaac
     8401 ataataaaaa atattccctc tccaaaaatc tctccttttt atccacgata taataattat
     8461 tcaaaggaga actcattcta aatataagca actaacaata actaacaatc cgccagctgg
     8521 tggatatttc actggggttc gttgcaaaaa acatagaata gattagcaac tgacaataac
     8581 taacaaaaag agatatttta gttattttct tttgttcgtt tagttcgttg caaaaaaaat
     8641 tatgaatatc ttcaaaaaat accccccctt caagttcatt cttgcctttt ttgatctttt
     8701 ggtgctaaat gcatcctttg caattgcact aagactgcgc tttatgccgc atatagatgt
     8761 tataaatatc cccgagttta aaatcgctca agaatgggca atttttatcc cttattccct
     8821 tctttggatt gtcatatttc aatatcttgg actatacaaa agacatatta tctttacgaa
     8881 atcaaagcaa actgttctga ttaccaaagg gataattatt ggttttttca ttctaattct
     8941 tgctcagttt attataagac cttcagttat gctcgatagc agactgatgt tgatttactt
     9001 tctactgcta tgcacagttc tgttaatatc atacagactt cttctcattc gcccaatatt
     9061 ccgcagtaaa tggctcggca atctgctaag cgaaaatgtt ttaataatag gcgctggaaa
     9121 gcgaggcaaa ttgctggctt ctaagattat ggaagagcag gaattccaat ctgaagtgat
     9181 aggcttcctt gatgataata ttgaaagagg tatgattgtt tttcaagatt tcaagattct
     9241 tgggaaatta gacgatatta agaaagttat aaaagattac agagtaaatt ccgt
//
LOCUS       DAOTJA010000026         9028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_7834_length_9028_cov_7.080798, whole genome shotgun sequence.
ACCESSION   DAOTJA010000026 DAOTJA010000000
VERSION     DAOTJA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7834_length_9028_cov_7.080798"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..458
                     /locus_tag="P5241_02515"
     CDS             192..458
                     /locus_tag="P5241_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32405.1"
                     /translation="MSCSKLQCLPQNIITTTISTIQNTTSNTSKNNQDSNIITIQDSA
                     NEYYVTPNNNKELRNLLFSINKIHKVAPYTILESSGGDPAINGD"
     gene            537..722
                     /locus_tag="P5241_02520"
     CDS             537..722
                     /locus_tag="P5241_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32406.1"
                     /translation="MNIKNIKTEPGNDGIILIINAEQDNIDEQTSNIQLDTIELRMRF
                     DSLTENLYIQKKIINSR"
     gene            1132..1737
                     /locus_tag="P5241_02525"
     CDS             1132..1737
                     /locus_tag="P5241_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32407.1"
                     /translation="MSFPDNKTMRIDVTKDFNPEDNTPLFTLNNNINLKIKSQSSIRD
                     SNKPLENEVWRLNRLYLDNDNSNTISNGDICINTEPPACIALEGAICPGPVPYGLVNC
                     NTNYECGRPLYSFTKPERIKTTNPNGNYIYEQDGLYLDNDNNNKVSDKDLRMSVGDSP
                     AEGSDYCGKEPYSCIREVTRDVLTVYFKIIGHTGPSRGVFE"
     gene            2221..2424
                     /locus_tag="P5241_02530"
     CDS             2221..2424
                     /locus_tag="P5241_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32408.1"
                     /translation="MLLAKGNGNATITKTNTSTSTSSTTTTSSTTTTSSTTTTSSTTT
                     TTSKPPINNQCEAIYFNELSAAP"
     gene            complement(2930..3076)
                     /locus_tag="P5241_02535"
     CDS             complement(2930..3076)
                     /locus_tag="P5241_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32409.1"
                     /translation="MSFSIVVVVEEVFNNSELGFVILFRSIKQLINAIKIIDIISIFL
                     FSII"
     gene            3306..3821
                     /locus_tag="P5241_02540"
     CDS             3306..3821
                     /locus_tag="P5241_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32410.1"
                     /translation="MLFSVYNGRFESKSELKFSNINTNIIKILKNNKEYNFEGLSSLP
                     QTKIVNVKIYLEIPKATAKSSYFTLLLPIALAKISPDANEYQYNPEYPISLGDTILPE
                     SPILKHRIYKIIFQEPIFEQRILKGARGDIAADFDGEYLFDRDKDAKLFIKFANSQDW
                     INIAWGDESPY"
     gene            complement(4049..4282)
                     /locus_tag="P5241_02545"
     CDS             complement(4049..4282)
                     /locus_tag="P5241_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32411.1"
                     /translation="MNNIQIYKFFKFTPSYLKDNVVASIKHWLSKSKFFSFPQLIAPV
                     KDRIPFSVHSSLLLRCNLGLSAINFEISINWSL"
     gene            complement(4403..5923)
                     /locus_tag="P5241_02550"
     CDS             complement(4403..5923)
                     /locus_tag="P5241_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRV32412.1"
                     /translation="MVVGTHSLIQKDVQFGNLGFVIIDEQHRFGVAQRSLLLRGSTQI
                     TEQINAKTTIINTKQKTDLPYKELTDKIKGVIFDVFNKLGSGLQKLVYQEALTKALSK
                     QNISFDNTKSLNVVYDNKKIGLYKPDFIIENKIILVITSRSFSDKTEEKQVWSYLKES
                     GFKLAVLVNFNNDNIEIKYVVYDLACDLKNIDTNQRIYSPELAFYPHLLSMTATPIPR
                     TLALAMYGDLELSILDEMPKGRQSIITKLVSKRSEDKMYDFIKKQIIEGRQAFIICPR
                     IEDTSTKQAPTQISTQTSINNVYYDDKSNSKDKTQSQLLIHKPNIFSPSQILDYEVKA
                     VKKEYERIKKIFPEFQIEMIHGKMKPKEKDDIMRKFYDNKIQILIATSVIEVGIDVPN
                     ATIMVIEGAEHFGLSQLHQFRGRVGRGEYQSYCFLFTESYSKTTRERLKAMLQYSDGF
                     KLAEIDLHLRGPGEFFGVNQSGFPDLAMQALSDTKQLAQIHEEATRLINKDNTLQS"
     gene            complement(5924..6232)
                     /locus_tag="P5241_02555"
     CDS             complement(5924..6232)
                     /locus_tag="P5241_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV32413.1"
                     /translation="MIQDIETGIPMNRLLEGDVGSGKTVVAISIAYLCLKNGFQVAFM
                     APTEVLVKQHFQTIKQLLKPFDIKIACLTSDGVEIKDGFLEGKVSKEYLLKSIAINTP
                     "
     gene            complement(6236..7063)
                     /locus_tag="P5241_02560"
     CDS             complement(6236..7063)
                     /locus_tag="P5241_02560"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32414.1"
                     /translation="MLQINDKINNLRGIGPYYETKLKDLGIITIRDLLFYFPFRYQDF
                     TNIKNIADLVANEQATIIAHITKIKSYRTFKKHMLLITATVEDESGSIEVLWFNQPFI
                     IKTLKQGTTVSLSGKVKQQGKKLLLESPAYELVSQDDDFNIQDLRHTGRLVPIYSETA
                     GISSKMIRSYIKRALKMFLDKIPESLPLETRQNYNLMDIKNALSQIHFPDSQELANEA
                     KRRFVFEEMLLVQIHLLNLKNRLSQFRAPKIPKDINAIKEFISYLPFKLTNAQRKAI"
     gene            7168..8919
                     /gene="pyrG"
                     /locus_tag="P5241_02565"
                     /pseudo
     CDS             7168..8919
                     /gene="pyrG"
                     /locus_tag="P5241_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
     gene            8954..9026
                     /locus_tag="P5241_02570"
     tRNA            8954..9026
                     /locus_tag="P5241_02570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8987..8989,aa:Lys,seq:ctt)
BASE COUNT         3416 a         1355 c         1272 g         2985 t
ORIGIN      
        1 gttttaatgt taatttgaca tcttagaaat attttttaat acaattatcc aaatttctta
       61 aaaaatattt ctaagatgtc aaattaacat taaaactaca aaaaatagat atgaaaaatt
      121 tttgaattat tattttaact attattatca ctttatcagt ttataacatt gtcttaataa
      181 attttaaaaa tgtgtcttgt tcaaaattac aatgtttacc tcaaaatatt ataacaacta
      241 caatttcgac aattcaaaac acaacttcca atacatctaa gaacaaccaa gacagcaata
      301 taataacaat acaagacagt gccaatgaat attatgttac acccaataat aataaagaat
      361 tacgtaatct tttgttttca attaacaaaa ttcataaagt agctccatat actattttag
      421 aatcatcagg gggagatccc gcaataaatg gggattgaat tcatattaaa tatgaaaaat
      481 gcgatcagtc ttataatcag tgcgaaatat atttttatga taatgtaatc gatatattga
      541 acatcaaaaa tataaaaact gagcctggca atgacggtat aattcttatt ataaacgcag
      601 aacaagataa catagatgaa caaacttcca atatccaatt agatactatt gagctacgaa
      661 tgagatttga ttctttgaca gaaaatcttt atatacaaaa gaaaattatt aatagtcgat
      721 aattaaaaat ttaaaaataa tttaaaaaat atgaaaaaaa tatattatat atgttttatc
      781 atttttagct taattatttt attattaggt ggcctaaata aaatgtttac tgcacaagca
      841 gcagcaccac gtggtaaatt tggatgtaac attctattag gcgacaattg taacaattga
      901 gaaagccgat atatagatat taatgttgga gcaatctcgt cttttgcata tagactagaa
      961 ataactaatg gctatccaaa tgatataaag gctaacacag atgttattat taaaatccct
     1021 aattcagtta atgctatttt tgatacaaaa taccaagaac caagtcctta ttctaccgct
     1081 tacgcattat atggaggtta cgcattatat ggaggtaagg ttgataaaaa tgtgagtttt
     1141 cctgataata aaaccatgcg gattgatgtt actaaagatt ttaacccaga agacaacact
     1201 cctttattta cattaaataa taatataaat ttaaaaataa aatcacaaag ttcaatcaga
     1261 gatagtaata aaccactgga aaacgaagtt tggagactta atagattata tcttgataat
     1321 gataacagca atactataag taacggcgat atttgtatta atactgaacc acctgcgtgc
     1381 atagcattag agggcgcaat atgccctggc ccagtaccat atggattagt taattgtaat
     1441 actaattatg aatgtgggag acctttatat tctttcacaa aaccagaaag aatcaaaaca
     1501 acaaacccga atggtaatta tatttatgaa caagatggac tttatcttga taatgataac
     1561 aataataaag tctctgataa agatctccgt atgtcagttg gcgattcacc agctgaagga
     1621 tcagattatt gcggtaaaga accttattct tgtattcgag aagtaactcg tgatgttcta
     1681 actgtttatt ttaaaattat agggcatacg ggaccaagta gaggtgtttt cgaatgatca
     1741 gtagatggag gtataactta tctaccagtt actaacccgg catttggtat taatgtaata
     1801 aataataata aaattaaaac tttaactaat attgctatta attcgaataa ctcatcaata
     1861 aataaccaat ctaattatac tattagtttt gcattaccaa ctgattcaaa cgcatataac
     1921 agcggagagt ttggtattat accctatgat ggctctatag ttattacctt tcctaaaaac
     1981 tttgatttaa ccaaagttag tttagctaat atttctaagg gcgaaggtcg ttgtaataac
     2041 aatattgatg gtggttttga tatatctatt aataaccaaa ctatagtttt aactcgcaag
     2101 ggagatggta caaattttat atgtccctac tcgaatatca taatagctct caataatatt
     2161 aaaaaccctc ctaatccagg aatctataca gtttctatag aaacaaaagc taagggcaat
     2221 ttgttgttag caaaagggaa tggcaatgct actattacca aaactaatac atcaacttct
     2281 actagttcta caactactac tagttctaca actactacta gttctacaac tactacttca
     2341 tccactacaa ctactacatc caaaccacct ataaataatc agtgtgaagc tatttatttt
     2401 aatgagttat cggcagctcc ttgagaacaa ggagaaaaat gcgataaatg tttttctgta
     2461 aaatgcgggg ataacatttg tgcttgtcgg gaaaaatacc gatgagaaaa atttatctgc
     2521 gaatcggggg aaaattgtat tgctctatat gcttcaggaa atcgtgatta ttgtccgtct
     2581 gattgctcct ccaaaacttc tactagttct acaactacta cttcatccac tacaactact
     2641 acatttccta ccataaaacc tctaaaacca atagacacaa acaaaatgag caaaatagaa
     2701 ttacaaaatt acattatgga actgcaaacc caattaataa atcttcttca gcaattatta
     2761 tttctgctaa agatgagaat acaataatga agaaaatatt ttgctgtgat aactaaatat
     2821 aatataactg ggtacaatat aatgtgaatt taattcgctt cactcattaa actcaaatta
     2881 tataactaat acaatataat ttgaatttaa ttcgcttcac tcattaaact caaattatgg
     2941 aaaataaaaa aatactaatt atatcaataa tctttatcgc gttaattagc tgttttatac
     3001 ttctaaataa aataacaaaa cctaattcag agttattaaa tacttcttct actactacta
     3061 caatactaaa agacactgcc aacaataaag aatttacaga tttctgagat gattatgatt
     3121 gaccaaaaag taaatttaat atactcaata ctccaaaaac cattacaaca gaatccttag
     3181 acattaaggg acaacctcct ggggaaccac caatttatta tgaaaaagaa acccttaatg
     3241 gtatagaata tgaaacatta actggctggg ttaaaaaaga cactattaaa ataaataatg
     3301 gacaattgct attcagtgtt tataacggaa gatttgaatc gaagtctgaa ttaaaattta
     3361 gtaatattaa tactaatatt ataaaaatac taaaaaataa taaagaatat aattttgaag
     3421 gtctttcatc tctacctcaa accaaaatcg tcaatgtcaa aatatattta gaaataccaa
     3481 aagcaacagc aaaatcgtca tattttacct tgctattacc aatagcttta gcaaaaatat
     3541 cccctgatgc taacgagtat caatacaatc cagaatatcc aatatcctta ggtgatacta
     3601 tcttgcctga atcacccatt ctaaaacata gaatttataa aattatcttt caagaaccaa
     3661 tattcgaaca aagaatactc aaaggagctc gaggggatat agcagctgat tttgatgggg
     3721 aatatttatt tgatagagat aaagacgcaa aacttttcat aaaatttgct aattcacaag
     3781 attggattaa tatagcatgg ggagatgaat ctccttattg agctttagaa aatttattag
     3841 attatgcaag taaaaacgaa acctacattg aattctattg ttcaaaattt aataattcaa
     3901 gtttaattat tcatacaaaa actgtctgag aatttaattt taattttcaa gaaaatgaag
     3961 atattaaaat tcctattcaa tatacaagct cagagccaca acaatataaa tgagtatctg
     4021 ataaaacaat tatgtttaat gacgaacatt ataatgacca attaatagat atctcaaaat
     4081 ttattgcgga taatcccaaa ttacaacgca ataataatga agaatgaaca gaaaagggta
     4141 ttctatcttt tacaggagca attaattgcg gaaaactgaa aaatttagat tttgatagcc
     4201 aatgttttat agaagccaca acattgtctt ttaaatagct cggggtaaat ttaaaaaatt
     4261 tataaatttg tatgttgttc aataatttat tttaaagaaa tcaaaaaata tttttaaaaa
     4321 gacttgtttt taaacaagtc ttttttattt attccatatg aaacttttct aaaaattctt
     4381 ggtagcgttg tcataaattc ggtcaagatt gtaaagtatt atctttattt ataagtcttg
     4441 tagcttcctc atgaatttgt gctaattgtt tagtatcaga tagagcttgc atagctaaat
     4501 caggaaaacc tgattggttt accccaaaaa attctcctgg gcctcttaaa tgcaaatcaa
     4561 tttctgctaa tttaaaacca tcactatact gcaacatagc ttttaatctt tcccgagttg
     4621 ttttactata tgattctgta aaaagaaaac aatacgactg atattcaccc ctacctactc
     4681 taccacgaaa ttgatgaagc tgcgataaac caaagtgttc agccccttct attaccataa
     4741 ttgtagcatt gggtacatct ataccaactt ctattactga agtggcaatt agtatttgaa
     4801 ttttattatc ataaaatttt ctcataatat catctttttc ttttggtttc attttgccat
     4861 gaatcatttc tatttgaaat tcggggaaaa ttttttttat tctttcatat tcttttttta
     4921 cagctttaac ttcgtaatct aatatttggg atggtgaaaa aatatttggt ttatgaatta
     4981 gcaactgaga ttgtgttttg tccttagagt ttgacttgtc atcataatac acattattaa
     5041 tgctcgtctg ggtagaaatc tgtgttggtg cttgtttggt agaagtatct tctattcgag
     5101 gacaaataat aaaagcttgt ctgccttcaa taatctgttt ttttataaaa tcatacatct
     5161 tatcttctga ccttttagac actaatttag taataatgct ttgtctgcct ttgggcattt
     5221 catcaagaat gcttaattca agatcaccat acattgccaa tgccaaagta cgaggaatgg
     5281 gggtggctgt catagataat aaatgtggat aaaacgctaa ttcaggtgaa tatatacgct
     5341 gattagtatc aatatttttt aaatcacaag ctaaatcata tacaacatac tttatttcta
     5401 tattgtcatt attaaaattt actaatactg ctaatttaaa accgctttct tttaaatagc
     5461 tccaaacctg tttttcttca gttttgtctg aaaacgaacg agatgttata actaaaataa
     5521 ttttattctc tattataaaa tctggtttat atagaccaat ttttttatta tcatacacaa
     5581 cattaagaga tttggtgttg tcaaaagata tgttttgttt agataatgct tttgtcagtg
     5641 cttcttgata aacaagtttt tgtaatcctg atcctaattt attaaataca tcaaaaatca
     5701 cccctttgat tttatcagtt aattccttgt atggaagatc agtcttttgt ttagtgttga
     5761 taatggtagt tttggcattt atttgttcag taatctgtgt tgatccgcgc aataaaagcg
     5821 agcgctgagc aacaccaaat ctatgctgct catcaataat aacaaaaccc aaattaccaa
     5881 attggacatc tttttgtatc agcgaatggg taccaactac caatcatgga gtattaatag
     5941 caatgctttt taataaatat tctttagaca ctttgccttc taaaaaacca tctttaattt
     6001 ctacgccatc acttgtcaaa caagcaatct tgatatcaaa aggctttaat aattgtttaa
     6061 tagtttgaaa gtgttgtttt accaatactt ctgttggtgc cataaaggct acctgaaagc
     6121 cattttttaa acacagataa gcaatggata ttgctactac ggttttacct gacccaacat
     6181 cgccctctaa taacctgttc ataggaatac ctgtttcaat atcttgtatc atttctcata
     6241 ttgcttttct ttgagcatta gtaagtttaa atggaagata agagataaat tcttttatag
     6301 cattaatatc ttttggtatc ttgggtgctc gaaattgtga taatctattt tttaaattta
     6361 ataaatgaat ctgcaccaat aacatttctt cgaatacaaa tcgacgctta gcttcattag
     6421 ccaattcttg actatcagga aaatgaattt gtgataaagc atttttaata tccatcaaat
     6481 tataattctg acgagtttct aatggcaaag attctggtat cttatctaaa aacattttca
     6541 aagcgcgctt aatataacta cgaatcattt ttgaactaat accagcagtt tcggaataaa
     6601 taggtactaa tcttccagta tgtcttaaat cctgtatatt aaaatcatca tcttgcgaaa
     6661 ctaattcata agctggactt tctaataata attttttacc ctgctgtttt actttgcctg
     6721 ataaagaaac tgttgtacct tgtttcaaag ttttgataat aaatggttga ttgaaccaaa
     6781 gaacttctat tgatcctgat tcatcttcta ctgtagcagt aataagcagc atatgttttt
     6841 taaaagtacg ataacttttt attttagtaa tatgagctat tattgttgct tgctcattag
     6901 ctaccaaatc agctatgttt ttgatattag taaaatcttg ataacgaaac ggaaaataaa
     6961 aaagtaaatc tctaatagta ataataccga gatcttttaa ttttgtctca taataaggac
     7021 ctatacctct taaattgttt attttgtcat ttatttgtaa catattataa aaatatagta
     7081 agattattgt atcatatttt tgtatctgtt gcaatttttt aaaaaaattg atagtataaa
     7141 tatatagaaa cattttaaac caatagtatg acaaaatata tagttgtaat tggaggagtt
     7201 attagtggcg taggaaaagg agtagctacg gcatcaatag gaaaaatttt aaaagaatat
     7261 ggatattctg taacagcaat aaaaattgat ccgtatatca atgttgatgc tggtacttta
     7321 agacccactg agcacggaga agtatgggtt actgatgatg ggggagaaat agatcaagat
     7381 ctaggaaatt atgaaagatt tttagatata gatataccta aaaagaatag tatcacaact
     7441 ggacaaattt ataaaaatat aattgcaaaa gaaagaaggg gagattattt gggacaaact
     7501 gttcaattta ttccacatgt agccaatgaa ataaaaaatc gtattataca agcaagcaaa
     7561 ggatatgatt ttgctttaat agaaattggg ggagtagtgg gtgattatga aaacgtgccc
     7621 tttttatttg ctatgaaaaa ttgagaaaaa gaaataaaac caaacaacat aatatatata
     7681 ttagtaacgt acctattcgc accccgtcat ataaaagaaa tgaaaacaaa gcctactcag
     7741 caagctataa aaatgctcac agaatcttca ggtattgccc cagattttgt attatgtaga
     7801 tcagaagaac ctttggataa agtaagaaaa gaaaaaatag aaacatatgc taatatttca
     7861 tctgattata ttatttctgc accagatgta actactatat atcaagtgcc aataaatcta
     7921 acaaaagaaa atataggaag caaaattttg gataaattaa atgtaaaacc aaaaactaaa
     7981 ataaattttg ctcaatgaaa taaatgaata aataatatct tacaaccaaa acatacaatc
     8041 aaaatagcaa ttattgggaa atatataaat agtggagact ttactttgac tgattcttat
     8101 ttatcaatta aagaagcgct aacacattgt ggagcgcatc taaacactaa gatcgaaata
     8161 gaatgattgg atgcaaaaag tttagaaaaa aatcctaaaa atgtagatat aatcaaacaa
     8221 tttgatggta ttattgtgcc tggtggtttt ggtacagaag gaacagaagg caaaatatta
     8281 gctattaact atgcgagaac acataatatt ccatatttag gcttgtgtta tggtatgcaa
     8341 ttggcattaa tagaatttgc cagaaatgtc tgcaaaatga aaaacgccaa tactacagaa
     8401 ataaatccca atactcccta tcctataatt gattttatac caatgcaaaa agaatttata
     8461 caaaatcacg cttatggtgg aacaatgaga ttgggagcat atggagctat tataaacaaa
     8521 aagtcattaa tttataattt atataaaaaa accaaaagaa tagaaataga caaaaaacaa
     8581 ataaaaatct tacaaaaaga taaacagaac aaatttagat taggaaagtt caacaaaaat
     8641 acagatacta taattttaga aagacataga catagatacg aagttaaccc aaaatatgtt
     8701 gaaacattag aaaaagcagg aattaaattt actggctatc atgtacgcaa tgacggaact
     8761 aaacttatgg aatttatcga attaccacag cataaatttt ttgtagcaac acaagcacat
     8821 cctgagttta aatcacgatt aaacaatcct gcgcctttat tttggggttt tgtaaaagca
     8881 tgtttatcca aaacaaacaa aatatcaaat agcaaatagc aaatattcta ttttgctatt
     8941 tgatatgttc gttgcctccg tagctcagct ggtagagcag ttgcctctta agcaaatggt
     9001 cgcaggttcg aatcctgtcg gaggcaca
//
LOCUS       DAOTJA010000027         7787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_9203_length_7787_cov_4.882825, whole genome shotgun sequence.
ACCESSION   DAOTJA010000027 DAOTJA010000000
VERSION     DAOTJA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9203_length_7787_cov_4.882825"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..344
                     /gene="rpsK"
                     /locus_tag="P5241_02575"
                     /pseudo
     CDS             9..344
                     /gene="rpsK"
                     /locus_tag="P5241_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012628.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
     gene            502..1020
                     /gene="rpsD"
                     /locus_tag="P5241_02580"
     CDS             502..1020
                     /gene="rpsD"
                     /locus_tag="P5241_02580"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRV32415.1"
                     /translation="MVLDAKCKKCRANGKKLFLKGDKCFSDKCIMNRRPFPPGRDKSR
                     KQYRKTLSTYQQQLKNKQEIKLIYGLNEDNLKRFYNKAFLMSGSTPDNLAKLLEKRID
                     TVVFYLGIAKSRTDARQLVSHGHFLVNGKRHDVASAVLNVGSEIQIRPSSMKQESFKK
                     RLENVREANIPK"
     gene            1342..2004
                     /gene="rpoA"
                     /locus_tag="P5241_02585"
     CDS             1342..2004
                     /gene="rpoA"
                     /locus_tag="P5241_02585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRV32416.1"
                     /translation="MISLPMEIKKIEEGANRGTFQIDGLYPNYGVTIGNALRRVLLSS
                     LEGAAITYVKIKNAPHEFATLPGVYETVLDIILNLKGLRVKMFSDEPQILNLDVKGEK
                     EVKASDIQPNSMVQIINPNLHIATLTDKKTDLEIEMTVEKGTGYRPSEEVAREKLPVG
                     VILLDSIFSPVTKVNFVVEDMRCKGRVDYNRLIITVETDGTISPGEALQDASNILAQH
                     FA"
     gene            2017..2271
                     /locus_tag="P5241_02590"
     CDS             2017..2271
                     /locus_tag="P5241_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRV32417.1"
                     /translation="MSKQSVAKPNSEKNKENTIALDTKIEDLPLSNRSINALKRNNIK
                     TLGGLLKISQTAMKELEGLGQKSRQEILDFINNNNFQLKE"
     gene            2273..2623
                     /gene="rplQ"
                     /locus_tag="P5241_02595"
     CDS             2273..2623
                     /gene="rplQ"
                     /locus_tag="P5241_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRV32418.1"
                     /translation="MRHLNKGKKLHRKTDQRKALVKILTYNLVMKERITTTKAKASMV
                     KPYLERLITRAKKQDVNALRYVLSKVPEKAANKIYYDLAKRYIDRPGGYTRVLKIEKP
                     RVKDSAKMVILELV"
     gene            2692..2991
                     /locus_tag="P5241_02600"
     CDS             2692..2991
                     /locus_tag="P5241_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HRV32419.1"
                     /translation="MAVEVANILQNKNSADYAKNKVGNNIVVVENLSKVKFTGNKLKQ
                     KIYYHHTDYLGHLKHETLEFKFKNKTEKAFKDTVRGMLPKNSLRDKRLKHLIIKL"
     gene            3122..3499
                     /gene="rpsI"
                     /locus_tag="P5241_02605"
                     /pseudo
     CDS             3122..3499
                     /gene="rpsI"
                     /locus_tag="P5241_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            3790..3987
                     /locus_tag="P5241_02610"
     CDS             3790..3987
                     /locus_tag="P5241_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32420.1"
                     /translation="MDISRGNLLGVSSMVLLGLLVIAFFVSRITDFEVKTTQIILGAL
                     FVSLYYLILFCAYWSMFKVLS"
     gene            4008..5369
                     /locus_tag="P5241_02615"
     CDS             4008..5369
                     /locus_tag="P5241_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV32421.1"
                     /translation="MDFFAIKFNSLFMQAKYKKPKIDLEEVLLDRRALDRNGETLEKL
                     ETPISTKNIFIIFVVIGVCLFILYARVFYLSIIKGNFFYQLSETNRLRYIPIKAPRGI
                     IFDRYGKPLVENSLTFSLILSPRDILQNSKQDELTLKIANIFGLSFDDIKAKYTNRQL
                     SYVEPILLKTNVSIEEARKFESEITEQDKKGFYLVEDYSRYYVDPEAFSHILGYTGKM
                     DNIDIQENPQYPISDIIGKMGIEKYYEQYLHGVSGKQLMEINARSEINPNLGEIKAKP
                     GNNLITTIDKDLQLELYKDLKTTITNLHIHKGAGLAINPQTGEVLALVSFPSIDVNTL
                     TKGSPRADIDKMTSNIYMPFINRVISGLYAPGSTIKPLVAIAALQEHTIDPDKTIVDE
                     DAIVIPNPYDAKHPSIFRDWKNHGIVDMRKAIAYSCNIYFYALGGGYKDIEGLGIRRL
                     KQY"
     gene            5502..5939
                     /locus_tag="P5241_02620"
                     /pseudo
     CDS             5502..5939
                     /locus_tag="P5241_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
     gene            6362..6838
                     /gene="greA"
                     /locus_tag="P5241_02625"
     CDS             6362..6838
                     /gene="greA"
                     /locus_tag="P5241_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRV32422.1"
                     /translation="MIKAKYSLTKEALEKLKAELDNLKVQRKEAIARIKVAKGYGDLS
                     ENSEYEEARENQGVIETRIMELEEIIRNANVIQVANRTNKIVVLGSTVDLKLVDKPTD
                     DIKTVQIVTSIEASPATGKISQDSPLGKALLNKQVGDIVIVQTPKKDIKYQIVKIY"
     gene            7200..>7787
                     /locus_tag="P5241_02630"
     CDS             7200..>7787
                     /locus_tag="P5241_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRV32423.1"
                     /translation="MSFAFILMNCLYIGAYNRGFKEGARNISYTYIPNIRSINTPVKN
                     VKLDFNLFAEVLQTIATKYYDKASLNTEKLMEGAIKGMVNSLDDPFTEFLTVKDAEEL
                     NTELSGSFDGIGAEVSKKNGKIIIVAPLKNTPADKAGLMPQDEIVAVDGVSTYNLSVT
                     DVVTKIRGKKNTKVELTIMRNTWDKPKIISIVRSSI"
BASE COUNT         3026 a          897 c         1340 g         2524 t
ORIGIN      
        1 ttatttttgt gaattcaact tttaataata caataattca attggctgat gaatctggta
       61 atattttagc tacatcatca gctggagcga ttggtttttc tggtactaaa aaatctactc
      121 cttatgctgc ctcaaaagta gcagagactt tgatagaaaa atctaaaaaa tgatgtcctt
      181 ttgaagcagt agtaaaaatt aaaggtatag gtcgtggcag agaaactgct ttaagatctt
      241 tgattactta tggggcaaat ttaggaatta atattcgaac agtatatgat gttacccctt
      301 tacctcataa tggaccaaaa ccaccaaaag cacgaaggat gtaatattaa cagagcaata
      361 aacaatgcac aattaataaa tagttattag ctaagagaag tagaaatttg tattaatcta
      421 tatattaatc tgtgtgaata aacatttttg ggattgtatt tacttataaa atttaaaatt
      481 aaaaaagtat taatttatat tatggtatta gatgcaaaat gtaaaaaatg tagagcaaat
      541 ggtaaaaaat tatttctcaa aggggataaa tgtttttctg ataagtgtat aatgaatagg
      601 cgaccatttc ctccaggtag agacaaaagc agaaaacaat atagaaaaac tttatcaact
      661 tatcagcaac agctaaaaaa taaacaagaa attaaactaa tttatggttt aaatgaagat
      721 aatttgaaaa ggttttataa taaagcattt ttgatgtctg gatctactcc tgataatttg
      781 gcaaaattat tggaaaaacg cattgataca gtagtttttt atttaggtat agcaaaatca
      841 cgtactgatg caagacaatt ggtaagtcat ggacattttt tggtaaatgg caaaaggcat
      901 gatgttgcct cggctgtttt aaatgtaggt agcgaaattc aaattcgtcc tagttctatg
      961 aagcaagagt catttaaaaa acgattagaa aatgtccgag aagcaaatat tcctaaatga
     1021 attagctttg ataaaaacaa aggtattgcc aaaattgttg ctgagccaga tattagtgaa
     1081 ataggttttg attttgcgtc agtagtcgag agcttctcat aaaaaaacaa ataaatcaaa
     1141 ttaattatat aaacaagtat taatacaaaa aaggaaaagc aatttttatt ttctttgatt
     1201 tctatttttg acttttgagg tctagtgtta aaaacttaga aattatttat agaaataaaa
     1261 attagaattt gaatttttac ttttttggta gctatataaa atattaaatt ttgtatataa
     1321 taatttatta aatttattac aatgattagt ttacctatgg aaattaaaaa aattgaagaa
     1381 ggtgctaata gaggaacatt ccaaatagat ggtttgtatc ctaattatgg tgttactatt
     1441 ggtaatgctt tacgaagagt attactttct tctttggagg gtgcggctat tacttatgta
     1501 aaaattaaaa atgctcctca tgaatttgct actttaccag gagtctatga gacggtctta
     1561 gatattattt taaatcttaa aggtttgcga gtaaaaatgt tttctgatga gcctcaaatt
     1621 ctaaatttag atgtaaaagg agaaaaagaa gtaaaagcct cggatattca gcccaattct
     1681 atggtacaga taataaatcc aaatttacat attgctactt tgactgataa aaaaactgat
     1741 cttgaaatag aaatgactgt tgagaagggc actggttata gaccaagtga agaggtggca
     1801 agagaaaaat tgccagttgg agtaattttg ttggattcaa ttttttcgcc agtaacaaag
     1861 gttaattttg tggtagaaga tatgcgttgt aaaggaagag tagattataa tcgtttaatt
     1921 ataacagtcg agactgatgg tacaataagc ccaggagaag ctttacaaga tgcatcaaat
     1981 attttagcac aacattttgc ttgaattgct gatgaaataa gcaaacaatc agtagcaaaa
     2041 cctaattctg agaaaaataa agaaaatact atagcactgg atactaaaat agaggattta
     2101 cctttgtcta acagatccat aaatgcttta aagcgtaata atataaagac attgggaggg
     2161 ctactcaaaa taagccaaac cgcaatgaaa gaattagaag gtttgggtca aaaatctcga
     2221 caagagattt tggattttat taataataat aattttcaat tgaaagaata atatgagaca
     2281 tttaaataaa ggtaaaaaat tacatagaaa aacagaccag agaaaagctt tggttaagat
     2341 tttgacttat aatttagtaa tgaaagaaag aattacgact accaaagcca aggcatcaat
     2401 ggtaaaaccc tatttagaaa gattgattac acgagctaaa aagcaagatg tcaatgcttt
     2461 gagatatgtt ttgtctaagg tgccagaaaa agcagctaat aaaatttatt atgatttagc
     2521 caaaagatat attgatcgac cagggggata tacgagggtt ttgaaaatag aaaaaccacg
     2581 cgtaaaagat tctgccaaaa tggttatttt agaattagtt taaactttat aaatatggat
     2641 accaaaacta aaacatttat aattgatgca actaataaaa gctgaggccg attggcagtt
     2701 gaagtagcaa atatattaca aaataaaaat tcggctgatt atgctaaaaa taaagttggt
     2761 aataatatag tggtagtaga aaatttatca aaagttaaat ttacaggtaa taaattaaaa
     2821 caaaaaatct attatcacca tactgattat ttgggtcatt tgaaacatga gactttggag
     2881 tttaaattta agaataaaac tgagaaagct tttaaggata cagtgcgagg tatgttgcct
     2941 aaaaattcat tgcgagataa aagattaaaa catctaataa ttaaacttta atattatggt
     3001 tagcaagact aaagaaaaaa ctacaaaaac atcaaaaaca tcaggtaaga catcaaaaca
     3061 tattaaaccc aaaaatgttg ataaacctga aacaaaaata tcaaataaag aagaaaacaa
     3121 tattgagcgt tatgtagaag ctattggtag aagaaaaaca tccacggtta gagtgagggg
     3181 gtgaataaat aaaaaaggga ttactataaa tgatattact ctggaacaat attttcctaa
     3241 tatagagctt caagaaaaat tgcttgagcc attaaaatta actgatatgc taaacaaaat
     3301 aggtattact ataaaagtat caggcggagg tttaactgga caagcagagg catcaaggtt
     3361 gggaattgct cgattattgg ataaaatgtt cccagatact aatcatatat taagagtatc
     3421 agggatgttg actcgagacc caaggaaagt agaaaggaaa aaatatggtt tacataaagc
     3481 gagaagagcg cctcagtgaa gaaaacgata gcagtatgga taggataaaa ttatatagat
     3541 cttgttaaac gtataagaaa atcataacaa atcgcggatt tatttatccg cccaaagtta
     3601 attattatgt ttgccaaata tgtatataat accattttgt ttttattagt aatattaata
     3661 tcattagctg tattacctaa tcttaatatt tattttcggc ctaatttgat tttaattttt
     3721 ttgattattt tattgtttag agaaccttta ttatttaatt taccattttg aatattagga
     3781 ggacttgttt tggatatatc gcgaggtaat ttattgggtg taagctctat ggtattgttg
     3841 ggacttttgg taatagcttt ttttgtttct agaattactg attttgaagt aaaaacaaca
     3901 cagattattt taggggcatt gtttgttagt ttgtattatt tgattttgtt ttgtgcatac
     3961 tggagtatgt ttaaagtatt gtcttgaaat atattgataa atagtttatg gacttttttg
     4021 ctattaagtt taatagttta tttatgcaag caaaatataa aaaaccaaaa attgatttag
     4081 aagaggtttt gttggatcgt cgggcattag atagaaatgg tgaaacatta gaaaaacttg
     4141 aaactcctat aagcacaaag aatattttta ttatttttgt agttattggt gtttgtttgt
     4201 ttatacttta tgctcgggta ttttatttaa gtattataaa aggcaatttt ttttatcagt
     4261 tgtcagagac taatagatta cgttatattc ctataaaagc accaaggggt attatttttg
     4321 atagatatgg gaaacccctg gtggaaaatt ctttgacatt cagtttgata ttatcaccga
     4381 gggatatatt acagaattct aaacaagatg aattgacatt aaaaattgct aatatttttg
     4441 gtttatcatt tgatgatata aaagcaaaat atactaatcg tcagttatcg tatgtggaac
     4501 caattttatt aaaaaccaat gtttcaatag aagaagctcg taaatttgaa tcagaaatta
     4561 ctgagcaaga taaaaaaggg ttttatttgg tagaagatta ttctcgatat tatgtagacc
     4621 cagaagcgtt ttcgcatata ttggggtata ctggtaaaat ggataatata gacatacaag
     4681 aaaatccaca atatccaata tcagatataa ttggtaaaat gggtatagaa aaatattatg
     4741 aacaatattt acacggagta tcaggtaaac aattaatgga aataaatgcg cgatctgaaa
     4801 taaatccaaa cttgggagaa ataaaagcaa aaccaggaaa caatttaata actacaatag
     4861 ataaagattt acaattagaa ctatacaaag atttaaaaac aactataaca aatttgcata
     4921 ttcacaaagg agcaggattg gctataaatc cccaaacagg agaagtattg gctttggtaa
     4981 gttttccatc tatagatgta aatacgttga ccaaggggtc gcctcgagca gatattgata
     5041 agatgacaag taatatctat atgccattta tcaatagagt aatatcaggg ttgtatgctc
     5101 cagggtctac cataaaacct ttggtagcta tagcagcatt acaagagcac actattgatc
     5161 ctgataaaac aattgttgat gaagacgcaa tagtgatacc taacccttat gacgctaaac
     5221 atccaagtat atttagggat tggaaaaatc atggtattgt agatatgaga aaagcaatag
     5281 cttattcttg taatatttat ttttatgctt tgggaggtgg atataaagat attgaaggtt
     5341 tgggtattag aagattaaaa caatattgag aaaaattcca tttagcaaaa gcgctgggga
     5401 ttgatttatt aggagaaaaa gatggtattt tgccagaccc agaatgaaaa aagacgcaca
     5461 ataaaacaga tcctacttga tatttaggcg atacttataa tgtatctatt ggccaaggtg
     5521 atttattaat aacccctatc caaattgctt actatatagc atcaattgcc aacaatggta
     5581 atattatgaa accctatatt gtgtccaaaa ttattaatga aaataatgct attgtttttg
     5641 ttaataatgc acaagtgttg aataaattgg atataacatt agataaattt aaagttgttc
     5701 aagagggtat gaggggggta gtaacaatgg gctctgctca aatgttaaac aatttaacag
     5761 atatgaaagt agcaggtaaa actggtactc cacaaattgc gggtggacaa aaaacaaatg
     5821 ctttttttgt agcttatgct ccatatgaaa atcctgaaat tttagttttg gtgttattgg
     5881 aagaacctcc cgaaggttca gtagtagcta taccagtagt agatcaagtt atgcgctgat
     5941 actataacaa tagatttcat aaataacaat atgatattaa acctattgtt aaattccaaa
     6001 aatactttga tataaatata atataatttt tggactataa caatagattt atcaaataaa
     6061 aatatattat acaatctatt gttaaattcc aaaaatactt aagaattatc gaaaatagtt
     6121 ataaattcat tggtgcacgc ttgacttgca atttttataa aagtatgcta cactaacaat
     6181 cattttatat tagcttatta tgagctataa taataaataa aatgccataa gcactgtttc
     6241 gggggtgctt ttaataaata tttatagtgt aattaagccc atataattca taaattatta
     6301 catttttggt aataattggt aaatagtcgg aaaattttta agatgttatt aaaattattt
     6361 tatgattaaa gcaaaatatt ctttaacaaa agaagcatta gaaaaattaa aagcagagct
     6421 ggataattta aaagttcaac gtaaggaagc tattgcgaga attaaagttg ctaagggcta
     6481 tggtgattta tcagaaaatt ctgaatatga agaggcacgt gaaaatcagg gcgttattga
     6541 aactcgaatt atggagttgg aagaaattat tcgcaatgct aatgttattc aagttgccaa
     6601 tcgtactaat aagattgtag tattggggtc aacagtagat ttgaaattag tagataaacc
     6661 aacagatgat attaaaacag ttcaaatagt tacttctata gaagcatcgc ctgcaacagg
     6721 taagatttcg caagattcgc ctctgggcaa agctttgtta aataaacaag taggagatat
     6781 tgtaattgtg caaactccta aaaaagatat aaaatatcaa attgttaaga tttattaaaa
     6841 gtgtcacaat gaatggctat atataaaaca gcttttgttt tggaataaaa gctgtttttt
     6901 aatttttaaa acactttttg acaaaaacac ttttttaatt tatagtatca aaagtattgt
     6961 taaattcaaa aaaatttgat atacttataa tcatataaaa agttaaaaca aaagttcggt
     7021 taaaaaacaa tatattatga atataaaaga tttattatga atgtaaaaga taaacttttt
     7081 tattatgaat ataaaagata aactttttta ttatgaatat aaaagataaa atttctaaaa
     7141 tatgagcaga ttgcaaacaa acatttagta aaagagattt ttggtttttt gttatttttt
     7201 tgtcgtttgc ttttatttta atgaattgtt tgtatattgg tgcttataat agagggttta
     7261 aagaaggcgc ccgtaacatt tcttatactt atataccaaa tattagaagt ataaatactc
     7321 cagtaaaaaa tgtaaaatta gatttcaatt tatttgccga agtgttacaa actattgcta
     7381 caaaatatta tgataaagcc agtttgaata cagaaaaatt aatggaagga gctataaaag
     7441 gaatggtaaa ttcgctggat gatcctttta cggaattttt gaccgttaaa gatgcagaag
     7501 agctgaatac agaattgagt ggttcatttg atggcattgg agcagaagtg agcaaaaaaa
     7561 atggtaaaat tataattgtt gcaccactta aaaatacacc tgctgataaa gctggtttaa
     7621 tgcctcaaga tgaaattgta gcagtagatg gagtctctac atataattta agtgtaacag
     7681 atgttgtgac aaaaattcgt ggcaaaaaga ataccaaagt agaacttaca ataatgcgta
     7741 atacttggga taaaccaaaa ataatttcta ttgttaggag ctctata
//
LOCUS       DAOTJA010000028         7757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_9236_length_7757_cov_5.421579, whole genome shotgun sequence.
ACCESSION   DAOTJA010000028 DAOTJA010000000
VERSION     DAOTJA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_7757_cov_5.421579"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="P5241_02635"
     CDS             complement(<1..306)
                     /locus_tag="P5241_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV32424.1"
                     /translation="MDYYDILGVPPNASDEDIKRAFHKLAYQYHPDRPGGNEQKFKEI
                     NEAYQVLSNKKTRTQYDTYRRYGNNNFQQGFSGFQDNSQNTDFDFSNFGFGNQSFGFD
                     "
     gene            complement(423..857)
                     /locus_tag="P5241_02640"
     CDS             complement(423..857)
                     /locus_tag="P5241_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32425.1"
                     /translation="MFVLYQEYTLKTQNTKFSLLDYSFFVIEKTSSFMPFKPPINNTI
                     DNLLTNTMSNSFINNYIGVSQLGALGTNTKNINDLLKNNVSSILHIKITGQETISDII
                     KQYALSASTTTKYIISAILIIIIFGICNLISILLNIIIVPLG"
     gene            complement(1439..3394)
                     /gene="dnaK"
                     /locus_tag="P5241_02645"
                     /pseudo
     CDS             complement(1439..3394)
                     /gene="dnaK"
                     /locus_tag="P5241_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(3472..3864)
                     /locus_tag="P5241_02650"
                     /pseudo
     CDS             complement(3472..3864)
                     /locus_tag="P5241_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
     gene            complement(3883..4008)
                     /locus_tag="P5241_02655"
     CDS             complement(3883..4008)
                     /locus_tag="P5241_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32426.1"
                     /translation="MNKNKDIIDNQSTKSTPIQNTDINNNNIEELKKQCEEYLNN"
     gene            complement(4469..4684)
                     /locus_tag="P5241_02660"
     CDS             complement(4469..4684)
                     /locus_tag="P5241_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32427.1"
                     /translation="MRQQLSPRQKQLLYYLAEEQIKNYSPISSAQIKEKYKLDFSPAT
                     IRHELFELCQMEYLEQPYTSAGRILSD"
     gene            complement(5399..5545)
                     /locus_tag="P5241_02665"
     CDS             complement(5399..5545)
                     /locus_tag="P5241_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32428.1"
                     /translation="MKKTPLSLIDDPEFLSKIEDKTYQILEPVKRTIDIILGLTLFLI
                     TLPL"
     gene            complement(6228..6575)
                     /locus_tag="P5241_02670"
     CDS             complement(6228..6575)
                     /locus_tag="P5241_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32429.1"
                     /translation="MVNNNQYQEGLQYLKKAIKTGPIFNSFQSILNGIDTFIKVGDID
                     TALQIAQYTEKQIKNEPALYLKLAQIYLQQKNEKEALNSILRAKYLYDIYPTLKTPKI
                     DEEMQSIMQKLSK"
BASE COUNT         2570 a          956 c         1131 g         3100 t
ORIGIN      
        1 atcaaaacca aaactttggt taccaaaacc aaaattagaa aaatcaaaat cagtattttg
       61 cgagttgtct tgaaaacctg aaaaaccttg ttgaaaatta ttattaccat atcgtcgata
      121 tgtatcgtat tgagtacggg tttttttgtt agaaagcact tgatatgctt cgtttatttc
      181 tttaaatttt tgttcattgc ctccaggtct gtcaggatga tattgataag ccaatttgtg
      241 aaaagcgcgt ttaatatctt catctgaagc attgggggga actcctaaaa tatcataata
      301 gtccataatt aggaattata ttatttgcta atttattctg attgtatact aagaatttgt
      361 tgttcgacat ttatagtttt aaatttgaaa aattttgttc ataaaagaat ttgtaatata
      421 aatcagccca aaggtacaat tataatattc agcaaaatag atattagatt acagatacca
      481 aaaataatta taattaaaat agcgctgatt atatatttcg ttgttgtgct agctgataag
      541 gcatattgtt ttataatatc actaatagtc tcttgaccag taatttttat atgtagtatt
      601 gaactgacat tattttttaa taaatcattg atgttttttg tatttgtacc taatgctcct
      661 agttgcgata ctcctatata attatttata aaagaattag acatagtatt ggttaaaaga
      721 ttgtctatag tattatttat aggaggttta aatggcataa aagatgatgt tttttcgata
      781 acaaaaaatg aataatctaa taacgagaat tttgtatttt gtgttttaag ggtgtattct
      841 tggtataaaa caaacattga aaaaacaata atactaaata ctatatagtt agctccagcc
      901 ttgataaaac ggcgtcaatt gaatttaata taatttgtgg cttcgttatg catttgatat
      961 gaacctcaaa aaataagcaa tattgaacac ccaagaccta aaagagaata taaatttatt
     1021 tgtagaaaag atttataaaa tgggcaagca ataccaagca aaattaaaca tattcacaca
     1081 aatagttttt tgttgtcagt gacgaatagc ataaaaaaca aaaaaattaa tccaccaatt
     1141 aaagatagta caacatttat tattgtctgt atagatggtt gtatagttgt cattagcttt
     1201 gataattgag tgctaaatat aataaaaaat atcaaagata taacacctaa aatattttgt
     1261 tttattattg acaaggtatc ggcaagatga gcttggtata atttattaaa atcactagat
     1321 attttatttt gcatttaatg atttattatt agtatattaa ttagtctaat aatagcacaa
     1381 aattaaaata aaatctaccc cattagaaag tttttacaat ttctaatggg gtcagaaatt
     1441 atggcttttc ttcaaagtct gtatcttgaa tattatcttc gtcggattga tcattttgtt
     1501 ctgtttctat gttttgatta attgtggtat ccgaagtttg tgtttcgtct tttttatata
     1561 aataagaacc aatctctgtt aaactttttg agagatcatc tataccctct ttgataagtt
     1621 tgatggattc gtctatatca gtagtttctt tattatatag ttgttctaag tgagaaattt
     1681 tttctgtaat ttttgttttt aattcatctg gaattttatt ttctgcttct tttaaatttg
     1741 tttttgatga atatactaaa ttttcagctt gatttttgag ctctattaat tctttatttt
     1801 ttatatcatt ttgggcaaaa gtttgagcct cttgtttcat tttttctatt tcttctttac
     1861 taagattggt ggaagcttct atttttacag attggctttt attagtagct ttatcaacgg
     1921 ctgaaacatt taatattcca ttacggtcaa tatcaaaagt tacttcaatt tgtggtactc
     1981 ctcttggtgc aggcataata ccatttaaaa caaatgttgc caaagtttta ttgtcttttg
     2041 ccaaaggtct ttctccttga gttacatgaa tttctacagc aggttgattg tcagtagcag
     2101 tagaaaagat ctcggttttt ttagtaggaa tatatgtgat tttgggaatc attggagtgg
     2161 ctataccgcc aagagtttct attgataaag ttaaaggggt aacatctaat agtaataagt
     2221 cttttacttc tccttgtaaa gcacctgcta caatagcagc acccgtagcg actacttctt
     2281 caggattaat atctttttta ggctctttac ccaaaagttc ttcaattttt ttctggatta
     2341 taggaattct tgtagtacca cctactaaaa taatatcttg aatatccgaa agatgcaatt
     2401 tggaatcagc aatagttttt ttaagacatt caatagatcg gtctactaaa tcttttataa
     2461 gatcttcaaa tttagaacga gaaattttta ttagtaaatg ttttggtccg tctgcagtag
     2521 aggtaataaa tggtaaatta atttctgttt ctggtatagt agataattcc cttttggctt
     2581 tttcagcagc atctttgatt ctttgcaatg ctaatttgtc ttgggttaaa tcaataccat
     2641 cagatttttt gaattcagta ataatatagt ccattaattt tttatctaaa tcatcaccgc
     2701 ccaaatgtgt atctccgcca gtgcctaata ctgaaatatt ttctccgaat gttccttgtt
     2761 cgtcactagt gcctacgact tctataatag agacatccaa agtaccacct ccgaaatcaa
     2821 atactagaat tttttcttgt tttcttttat taaaaccata agcaactgct gcggcagtag
     2881 gttcattgag aattcttttt acttttaaac ccgcaatttc cccagcattt ttagttgctt
     2941 gtctttggga gtcattaaaa taagcaggta cagtaattat agcttcttca atagtttctc
     3001 ctaattttga ttctgcatca ctttttaatt tttgtaaaat catagctgaa atttcttcag
     3061 gggtatatca acggtcaccc atttttattt ctactccatg tgtgacagat tcacgagttt
     3121 cataaggtaa tgatttaata tcatcttgtg tttctggatc ttgaaatctt cgtccgataa
     3181 atcttttgac tgaaaaaata gtattttgag gattggtaat tgcttgtcgt ttagctaatg
     3241 ttcctactaa acgttcttgg ttttttgaaa tagcgaccat agagggcatt gttctttcac
     3301 cttcacttgt ttcgataatt tttggttgtc cgttttcaaa aacagcaact tctgaatagg
     3361 ttgtacctag atcgattcct aatattttag acatattgta tagtttaaat aatttttaat
     3421 ttataatttt aattttgtaa taataagcta atctttattg agcgaccttt attatttatt
     3481 gttaataact ttgacttttg atggacgtaa taccatatct ttatataaat atcctttctg
     3541 aaattcttct attattaagc catcttcttt atttggatct gtttgaactt ctatagcctc
     3601 gtgcatttta ggattaaatt gttggtttag ggcctctatt ttaactactc cctgtttctt
     3661 tagaaaatta tctaattgag aataaattaa ataaaatcct tctttcattt gttttcattt
     3721 aggatcatta tcgttatttc ctaaggcatt taaggacaat tcaaaactat ccataatatt
     3781 tagcaaatct tctataaaag acattttttg caaattagca aaatgttcga atctttcttt
     3841 ttcattttgt tgataattga gaaaattggc tttttcagta tgtcaattat ttaggtattc
     3901 ttcacactgt tttttaagtt cttcgatatt attgttattt atatctgtgt tttgtattgg
     3961 cgttgattta gtagattgat tgtctattat atctttattt ttattcatta tattaattta
     4021 aagaatttat taagcttttg aatattttga agtgttgttc ataacgtgtt actttggggg
     4081 taataattcc aactattaaa ttggtattat ttgattttgt tgctataaaa ctaaatgtct
     4141 ttgtgtgtat aagcggattg tttttaccaa taaatacatt aatttgtctg tcgtgtaata
     4201 tattctgaat tcctaatata gtattatcaa tattatcgat tgatttggca atagaatata
     4261 aatgtattgt attattaata attttatcat taaagatata ttctagccct gttgtataaa
     4321 tatcattatt ttttcagttg tatgcgagac taaaagattt tgttttttta gatatatatt
     4381 tagttagatc tttgatatca ttgaaagaaa tagataaatt atctggaaaa acattatcta
     4441 aaaaatcttg tattaagact tgtcagcctc aatcgcttaa aattctgccg gctgatgtat
     4501 atggttgttc taagtattcc atttggcata gttcgaataa ttcgtgtcta atagtagctg
     4561 ggctaaaatc taatttgtat ttttctttta tttgtgcaga gcttattgga ctatagtttt
     4621 tgatttgttc ttcagccaaa taatataata attgtttttg tcttggtgat aattgttgtc
     4681 gcatagtaat atttatattt ttaaataaga gattataaat aagaataaga gattacaaat
     4741 aaaatatata tttattataa ggtattttta agtattttag gtattatgac tatgagaata
     4801 attcttttta aaatatccct ataatattag caaattagca ctcttttgtc aagactgcca
     4861 actattttta tatattatgt caaaatgata gtaattaatg gctttttata tccaagggaa
     4921 tgtgtaaagt ttttaaaatt atcatcaagt ctaatcataa ggaatagtgt cttaaataat
     4981 ataaatcata tttaagtttt tcttttgtgc cttctacaga ataagagttt gaataatgaa
     5041 tttgtgctca accagttata cctggtttta caatatggcg aatattgtaa aatggaattt
     5101 ctttttcaaa taaagccaca agttttgctt cttctggtct tggacctact aatgatatat
     5161 caccctttat gacatttata aattctggga tttcatctaa atataatttt cttaaaaatt
     5221 tgccaaattt tgttatacgt tggtcatttg gtagagttca tctgggccca ttggaggcgt
     5281 ttttagtcat agaacgaaat ttgattaaat agaattcttt gtggtttttg ccaattctct
     5341 tgccaatata aaatatagga ccaaaatcat ctattaaaat accaataatt ataattggtc
     5401 aaagaggtag agtaattaaa aatagagtta aacctaaaat aatatcaatg gttcgtttaa
     5461 ctggttctaa tatttgatat gttttgtctt caattttgga caaaaattct ggatcatcaa
     5521 ttaaagataa aggtgttttt ttcaaaatag attcataaaa atctataaaa tctataattt
     5581 gaattttgtt tcatagagca ttaaataaaa tatttttgat gttattgttt ggaaaatgtt
     5641 tgtcaataat tataacatca acattttggg actcaattag tcgtgtaagc tttcaataat
     5701 catctggatt gtctattcta tctactaatt tataaccaaa ttgggggtta ttgtttagaa
     5761 caatgctcaa atccaaagct atagcatctt ggctaataat aattatattt aatggttttt
     5821 gcttttgaat aaatatagaa aatcaaatat gatttagaca aatacacaaa aatgatagcc
     5881 ctcaaattaa taataaatta gttttaggtg ttataccaac aaaagaaaat agataaaaat
     5941 aaataatact aataaataaa tttagcaaag ctattctgcc aagtgatgat ataattttta
     6001 tgcctggaag agtaaaagta ttatcataaa aatggttaat ataaagtata ataattcaaa
     6061 ttataaaaac aggtgtaaaa ctctgtaaat gtattttcaa aatattccaa gaaaatgtat
     6121 tgtaaaaacg caatcaaaca attatatata atattatata cagaattatt atgtctgaaa
     6181 taaataataa aaatccttta tatttgtgta tgtgtttcat aaacacttta tttagatagt
     6241 ttttgcatta tactttgcat ttcctcgtct attttgggag ttttgagagt gggatatata
     6301 tcataaagat atttagcgcg caaaatagaa ttcaatgctt ctttctcatt tttttgttgt
     6361 aaataaatct gagcaagttt aagataaagt gctggttcat ttttaatttg tttttcagta
     6421 tattgagcta tttgtagagc tgtatcaata tcccccactt tgataaatgt atcaatacca
     6481 tttaagatgc tttgaaaact attgaatatg gggccagttt taatggcttt tttgagatat
     6541 tgtaggcctt cttgatattg attattattt accaaataat tacctaatat tcaaggggcg
     6601 atagcaattt tgggatatgt tgcatgaaat tgttttagga gtttaacagc ttctagttga
     6661 tcttgttttg atggtgaatt tataagaatt tgtagaaaat aaatatctgt atctggtaat
     6721 gttaatttta attgatctat ggatgttttg gtatattttg ccagctgaat atcttctggt
     6781 gttaaatttt tatttcaacg taatttagaa agaatattta ctaatttagc aatatataat
     6841 tttaaattta gtggttcttg tttaataagt ttgtctaatt gttgattgat aaatagatag
     6901 atatcttcga tctgtggttt agaaaattta gttagatctc cattacttaa aagttctaaa
     6961 tctccgtaaa taagaaaatt attgataaat gtatcggtat tgtatttttc ttttatagca
     7021 gtaatgcctt gttcaatttg tccagattgt ataagactct cagctttttt atgtttagta
     7081 gccgtaataa gtactaaaac gttataatta taaatagtaa aacaaagtat gatacatgct
     7141 attattaata ttatagtttg tagtcagtgg ggtacagtaa ttttggttgg tttaaatttt
     7201 gttttaatag gggtagattg tgaactaata aaagcaaaaa tcataaaaag aagtaaatat
     7261 ccttctataa catcaaaaag aaatatattt tgaataaaat aagataatat acaaatagca
     7321 agaataattt tgggtcaaaa atttatagaa tttatatttt ttaattcttt ataagcaaca
     7381 taaaaaatac ataaatagct aataagtcca ataatccctg tggtcacgaa tatttctaat
     7441 atcatattat gtactttgtc aaaacgattt tcaagtggta agacttctac tatgtctgta
     7501 ttaaaattac gttgataagc aatttcaaaa ttttctggcc ctcaacctaa tagtggtttt
     7561 tgtaaagctg attttatagc aatattgctg actattagtc gactagtaga ggaggcatca
     7621 aatattgtaa tagatgttag ccgttgtaat ggcataatat tttgtataaa tttagtatct
     7681 cgtaaactaa aaagtatgat tatagcagta atgaatataa caattaaaac agttgctcat
     7741 tttttgatgt ttatatg
//
LOCUS       DAOTJA010000029         7503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_9576_length_7503_cov_5.365602, whole genome shotgun sequence.
ACCESSION   DAOTJA010000029 DAOTJA010000000
VERSION     DAOTJA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9576_length_7503_cov_5.365602"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..1147
                     /locus_tag="P5241_02675"
                     /pseudo
     CDS             509..1147
                     /locus_tag="P5241_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
     gene            1565..2143
                     /locus_tag="P5241_02680"
     CDS             1565..2143
                     /locus_tag="P5241_02680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV32430.1"
                     /translation="MHNLPQSNPSSHNTINNSKTMTTYTTKPPILIDTNKSYSAVFKT
                     SEGEFTVELNVKDTPITTNNFVFLARQGFYNNTIFHRIMKNFMIQGGDPTGTGMGGPG
                     YKFDDEKIIGEYTRGTLAMANSGHNTNGSQFFIMHQDYPLSKDYVIFGKVVQGLDVID
                     KIANTPVQLSAQGELSVPINPPTIYSIDIIEK"
     gene            2216..2461
                     /locus_tag="P5241_02685"
     CDS             2216..2461
                     /locus_tag="P5241_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012518816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32431.1"
                     /translation="MERENRTRVIKIYCAKCKTLLYKYRKEGGDHLVKCYKDMILKDY
                     TNKDLKCPKCHNVFAREAFIHCRKANKIIQGHVIVQD"
     gene            2697..2861
                     /locus_tag="P5241_02690"
     CDS             2697..2861
                     /locus_tag="P5241_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32432.1"
                     /translation="MENKNLTIAFFILGLALIISAIFVAHTFYKVKALDNTLTVTGSA
                     KKQVKADLVK"
     gene            2898..3419
                     /locus_tag="P5241_02695"
     CDS             2898..3419
                     /locus_tag="P5241_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRV32433.1"
                     /translation="MKQGYQQMEQDLKKVLQFFKDNNISETNLLIPPLDVSEIYKYEK
                     DSGPKTYTLRQIIELQSNDINGITNLAKKTKELIDQEVFFTSDRLEYYYSKLPELRIE
                     LLGESIKDARARAEIIAKSSGKKVNIIKQASMGVVQVLPINSVNVSDYGEYDTSTIDK
                     EIMVTVRAVFTIK"
     gene            3438..4499
                     /locus_tag="P5241_02700"
     CDS             3438..4499
                     /locus_tag="P5241_02700"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRV32434.1"
                     /translation="MFYLSYPVKVFATSGSEDLAQQICSELEKRLPPPIIQNSPNLFS
                     KINVVRFSNDNLQVQVDNVRGHFVVIVHTQVAPVNDHLMELFALLDAVINSKPADVLL
                     VFPYMPYSRSDRKNQPRISTMSHRIAQIISKSFGIKKVLLLDPHDSHIKHYFEPAADE
                     ITSVYLFSNYIQKYIINKNLFQNNNKNDTVIVFADTGAAKRFSNIAYHLQLPVAYIDK
                     ERPDNTEVGRFKKVVGEVQDKKCILIDDEILTGGTVIGDANILMENGAKEIYMAAIHP
                     ILSSLKESEQNLINKLDSSSICQFIVSNSVPVEHKIKNCRKFTVLSVAPLLAEAINRL
                     VQNESITYLHDPANFNLYE"
     gene            4598..5944
                     /gene="metG"
                     /locus_tag="P5241_02705"
                     /pseudo
     CDS             4598..5944
                     /gene="metG"
                     /locus_tag="P5241_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
     gene            6232..6513
                     /locus_tag="P5241_02710"
     CDS             6232..6513
                     /locus_tag="P5241_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32435.1"
                     /translation="MYKLPGYYYIAIIVSVFIILVCIIFQRPKHIIIKPNVSVSTNTS
                     VSSTLPLVSSYKPSTVSDNKNQSLSKFLYKVKTAPSPKGLAFVPNGQEI"
     gene            6988..7215
                     /locus_tag="P5241_02715"
     CDS             6988..7215
                     /locus_tag="P5241_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32436.1"
                     /translation="MRHIVVDEKRGIMYISDMGKNNILKLNLANDSIVNFVKTDENPN
                     TIALSLDGNLLFVSCRGQNYSEDNYSIPGPE"
     gene            7225..7404
                     /locus_tag="P5241_02720"
     CDS             7225..7404
                     /locus_tag="P5241_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32437.1"
                     /translation="MIFDTNSGKLVDAIIGGNQPTALGVSPDGKVLVFSDFLDGILEF
                     YQIPNYNDLKNNRSP"
BASE COUNT         2756 a         1016 c         1171 g         2560 t
ORIGIN      
        1 aataataaaa aattttatga aaaaagaaat cacaataaat gatattcaaa aatgggagaa
       61 atcttttact aaaagaaagg ggatttctca agatgaaaac acagctatta aaatagctat
      121 gtttaagttg gcagaagagg tcggagaagt ggcaaaggca atattggaag aaaactgaaa
      181 agaagttccg gctgaagttt cggatgttat tgtttttgct tgtaagatag caaacatagc
      241 agaaaaatct cataaaaccg acaaacttaa taatgtttta caaaagaaaa taaattattg
      301 tgaaagtaga aaatacaatg ccaaaatcaa aaaattcgat aaaccaaaaa ataaagaatt
      361 taaataaaaa atataacaag taaagtatac acataaaaga gcagtgaaca tcattgctct
      421 tttaattttt taaaaaatat gctacattat attcattatg aaattttatt gatcaaaact
      481 aaaaaaacct gtattttgtt tagctcctat ggcagaaatt actactttac cttttagatc
      541 tatttgtaag caaatggggg ctgatatagt ttttacacca atgatttcct cggatgctgt
      601 tattcataat ttaacacata cactcaaact ggctcaattt aaaaattttg agcagcctat
      661 tattatccaa atttttggtt atgatggtaa gaaaatttta aaagctattc aaattttgga
      721 taaaaaatta catccagctg gttttgatat caatatgggt tgtcctgctc gtaaaattat
      781 taataataat tgtggtgcct ctcttattag aaatattaat caagccatag aaatagtaaa
      841 aataattcgt tataattttt ctggtcagct ttctattaaa actcgaatag gttgagataa
      901 atttgatatc ttaccattat taaaagaatt tgaagctatt ggtgtaaatg ctgttataat
      961 tcatggccgt actgttaaac aagaatattc tggtaaggct gattgaaggg ctataaactg
     1021 tatagccaaa gaattaaaaa ttcctgtaat tggcaatggt gatataacgg attggcagac
     1081 ggcatatttg agatcaagaa acgtctctgg cgtgatgatt ggtcagggag cattgggtaa
     1141 tccatgaatt tttcaagaaa ttaaaaaaca aaaggatatt ttagtatctt cacaagaatt
     1201 ggcaaaaata attcgaaaac aagcaaaatt aagtatccaa tatttaggaa aaacacaggg
     1261 cataaaagaa tttaggaaac atttaggctg atatttaaaa aatccaaaat ataaagaact
     1321 acgtaaacaa gcagttttgg tctcagatat taaagatatt gaaaaaattt tagaacaatt
     1381 gcataaaata taaaaaaatg atacaataat tatacattca taaattaata gtcaatataa
     1441 acttttatat atgaaaaaat ctattttaat atggttagtt attggggttg gtgttatatg
     1501 tgggatatat attttttgac aaaataattc taacaatatt aaacctacta acacttcaat
     1561 ctctatgcat aatttaccgc aaagcaatcc gagctctcat aatacaatta ataattctaa
     1621 aactatgaca acttatacaa ctaagccccc aatccttata gatacaaaca aatcttatag
     1681 tgctgtgttc aaaacaagcg aaggtgaatt tactgttgag ttaaatgtca aagatacgcc
     1741 cattactaca aacaattttg tatttttggc aagacaaggt ttttataata atacgatttt
     1801 tcatcgaata atgaaaaatt ttatgattca gggcggcgat ccaactggta ctggtatggg
     1861 agggccaggt tataaatttg atgatgaaaa aattataggt gaatatactc gtggtacttt
     1921 ggcaatggct aattccggac acaataccaa tggtagtcaa ttctttatta tgcatcaaga
     1981 ttacccttta tcaaaggatt atgttatttt tggtaaagta gttcagggac tggatgttat
     2041 agataaaatt gctaataccc ccgtacaatt atcagctcaa ggagaactgt cagttcctat
     2101 taatccgcca actatttatt ccattgatat aattgaaaaa taatattatt gtttactatt
     2161 tggttttata tcgccagtaa tatttaataa ttatataata tttttatatg ctaatatgga
     2221 aagggaaaat agaacaaggg ttataaaaat ttattgtgcc aaatgtaaaa cccttttgta
     2281 taaatataga aaagaagggg gcgatcattt agtgaaatgt tataaagata tgattcttaa
     2341 ggattatacc aacaaagacc ttaaatgtcc taaatgtcat aatgtttttg ccagagaagc
     2401 atttattcat tgccgcaaag ccaataaaat aatacaaggg catgttattg tacaagatta
     2461 aaatggtttg ccaaattcta tccaaattct atgtttatca agtttgataa actttaaaga
     2521 ttatgttata ttgaacataa aattacataa taattttaga aataaatttt tgtctaaaac
     2581 tctaagcata tatataatgc tgagatagat cctcctaatt ttagttagtg tttgattgta
     2641 aattttctat aaatatggta tgatattaac aataaaaatt ctaaattaat tttattatgg
     2701 aaaacaaaaa tttaacaatt gcttttttta ttcttggttt agctttaatt attagcgcta
     2761 tttttgtggc tcatacattt tataaagtca aagcccttga taatacttta acagtaactg
     2821 gttctgctaa aaaacaagtt aaggccgatc ttgtgaaatg acaaagcagt ttttctagaa
     2881 ctgttgtaga aagtgaaata aaacaagggt atcaacaaat ggagcaagat ttaaaaaagg
     2941 ttttacaatt ttttaaagac aataatatct cagagacaaa tttattaatt ccgccccttg
     3001 atgttagtga aatttacaaa tatgaaaaag attctggtcc caagacatat acgcttcgtc
     3061 aaattattga attacaatca aatgatatca atggtataac taatttagcc aagaaaacaa
     3121 aagaacttat tgatcaagaa gtatttttta ccagtgatag gttagaatat tattattcta
     3181 aattaccaga attaagaata gaattattag gcgaatccat taaagatgcg agagcgcgcg
     3241 ctgaaataat tgctaaaagt agcggcaaaa aagttaatat tattaaacaa gccagtatgg
     3301 gcgttgttca agttttgcct ataaactctg ttaatgtttc tgattacggc gaatatgata
     3361 cttcaactat tgataaagaa ataatggtta cagtaagggc tgtatttaca ataaagtaaa
     3421 aattttaaaa taaagatatg ttttatttat catatccagt taaggtattt gctacctctg
     3481 gatcagaaga tttggcgcag caaatttgtt cagaattaga gaaacgtttg ccccctccta
     3541 ttattcaaaa ttctccaaat ttatttagca aaattaatgt tgttcgtttt tccaatgata
     3601 atttgcaagt tcaagtagat aatgttaggg gacattttgt ggttattgtt catacccaag
     3661 tagcaccagt taatgatcat ttaatggaat tatttgcttt attagatgca gtaatcaact
     3721 ccaaacccgc agatgtttta ttagtttttc cttatatgcc ctatagtcgc tctgatagaa
     3781 aaaaccaacc ccgtatttct acaatgagtc atagaattgc ccaaataatt tcaaaatcct
     3841 ttgggattaa aaaagtgctt ttattagatc cccatgattc gcatattaaa cattattttg
     3901 agcctgcagc tgacgaaatt acttctgttt atctttttag caattatatt caaaaatata
     3961 ttattaacaa aaatcttttt caaaataata ataaaaatga tactgtgata gtttttgctg
     4021 atactggtgc agcaaaaaga tttagtaata ttgcttatca ccttcagctc ccagtggctt
     4081 atattgataa agaaagacca gacaatactg aagtaggtcg ttttaaaaaa gttgttggtg
     4141 aagttcaaga caaaaaatgt attttgatag atgacgagat tttaactggt ggcacagtta
     4201 ttggcgatgc gaatatttta atggaaaacg gagccaaaga aatttatatg gctgctattc
     4261 atccgatttt atcttccttg aaagaatcag aacaaaatct tattaataaa ctcgattcct
     4321 cttctatttg tcaatttata gtttctaata gtgtgcccgt ggaacataaa ataaaaaatt
     4381 gtcgtaaatt tactgtttta tccgtggcgc cacttttagc agaagctatt aatagattgg
     4441 tccagaatga atccattact tatctacatg atccagccaa ttttaatctt tatgaataac
     4501 ccatatcaaa acttaaatta tgaatattaa accttacaat aaaaaaaaga acaaacaaaa
     4561 aatatttatt gggcttgctt gaccttatgt caatggcgat ttgcacattg gtcatttagc
     4621 aggttattta ttacctgcgg atttttttgc caggtttaat cgtttaattg gtaatgatgt
     4681 tttaatggtg tctggtgctg attgtcatgg cacccctgtt tctttggaag ctgataaatt
     4741 tcaaattagc cctcaagaaa tagttgctaa atattaccaa gaacatctac atcttattca
     4801 aaaagcagat ttgagttttg atatttatac aaaaactact actgacaatc ataaacatat
     4861 agttcaagat gtgtttttaa aattgttaaa agatggttat attatacgca aaacctctaa
     4921 acaattttat tctataaaag aaaataaatt tttgcctgat agatatgttg aaggtgtttg
     4981 tccgttttgt ggcaatatag atacaagaag tgaccaatgt gatgcttgcg gtaaactaat
     5041 ttgtcctgac gaattaattc aacctcacag caagctcact cagagtccag taattttgaa
     5101 agaaaccgaa cattatttta tggactggac taaactaact ccatttttac aaaaatattt
     5161 tgcaacccat aaacacaatt ggcgcccttg aattcagaag gaaacagatc aatggctttt
     5221 taaaggctta cagccaacag ccattacccg tgatttggat tgaggtgttg aactgcccat
     5281 agatcaaatt cctaaaaata tgttgattga taatatagag accaaaagaa tttatgtgtg
     5341 gtttgaagct gttattggat atttatctgc ttctattgaa tgagatataa ataaatataa
     5401 agatttttga tataacacta aatcaaaaca ttattatttt atgggtaaag ataatttggt
     5461 ttttcacact ctgttttggc ctgggcaatt atgggcatat aataaaaaac ttcatttgcc
     5521 cgatgtttgc gctattaatc aatttttaac tcttgaagga caaaaatttt ctaaaagtca
     5581 aggtattatt attgatgccc aatatatttt ggataaatat gggcctgatc aagttaggtt
     5641 ttattttact gctattatgc cagaagaaaa tgatgccagt ttttcttgga ctgattttca
     5701 agctcatata aatgatgttc ttattggtaa ttttgctaat tttgttaatc gtgtgctaac
     5761 attatctaaa aatatatcta taaataaaaa tattcagata gacaaaaata ttttaaaaca
     5821 atcatctaaa agttatttag acataattaa atcattagac tctctgcatt ttaaagatta
     5881 ttatacagaa tttataaatt tttctagctt tgctaataaa tatatttcag aagcacagcc
     5941 ttgaaaactt aaagatgtag accccttaaa attttatcaa attatggtga attgtttgta
     6001 ttgtgttttg tgtttagatt tgtttgctaa gccaatttta accaaagggt ctgagacatt
     6061 agataatatg ttgggaatta caattgatgt atgacccaac gccaatgatc tacagcacta
     6121 tcttatatct ttattatcaa aaattactat tcaaaatgtt gcgccattgt atcagaaaat
     6181 agataatgat ataattgtcc aagaacagaa taaattatta aaaactaaat aatgtataag
     6241 ttacccgggt attattatat tgctattatt gtaagtgttt ttattatttt ggtttgtatt
     6301 atatttcaga gaccaaaaca cattattatt aagcccaatg tgagtgtttc tactaatact
     6361 tctgtgtcta gtacactgcc tttagtatct tcatataagc catcaacagt ttctgacaat
     6421 aaaaatcaat cgttgtctaa atttttatac aaagttaaaa cagcaccttc tccaaaaggc
     6481 ctagcctttg ttcctaatgg acaagaaata tgagtcactt ctttattaaa cacaaaacgt
     6541 ggggtagttg ttcatagtgg gaaattttat gatactctaa ccaatatagt tcttaataat
     6601 tatggaggtg tagaagtcgt ggtaaataaa aatggatcta aggtatatat tagtcaaatg
     6661 gagacaggct cggtgtttga aattgatgca aaaactaaaa ctattttacg tgtgtttaaa
     6721 accaatagta cctgaacaaa agttatcgtt ttgtcgtatg acgaacaaaa actctatgct
     6781 tctaattgaa gtggtaataa tgtatcagag tttgatttaa taacaggaaa attaatacga
     6841 aacatcaaaa cagtcaaaac tcctcgcagt atttatccta ccaaagacaa taaatatctt
     6901 tatgttgctg gttatgacaa aggagagcta caaaaaatta atttacaaga cgaaacaagt
     6961 caaattcttt acgatcaagg agtagctttg cgtcatattg tagtagatga aaaaaggggt
     7021 ataatgtata tttctgatat gggcaaaaat aatattttaa aattgaattt ggctaatgat
     7081 tctattgtaa attttgtcaa aacagatgaa aatcccaata ctatagcctt atctttagat
     7141 ggtaatttat tgtttgtatc ttgtcgtggg caaaattatt cagaggataa ttattcaata
     7201 cctgggccag agtgaggtac agttttgatt tttgatacca attctggcaa attagttgat
     7261 gctattatcg gaggtaatca acctacggcc ctgggtgttt ctcctgatgg taaagttttg
     7321 gtgttttcag attttttgga tggcatatta gaattttatc aaataccaaa ctataatgat
     7381 ttaaaaaata atcgaagccc ttgacttcaa gattacaaaa attttattaa aaaataatta
     7441 tttgttaaac aatgacacat cttgatttaa tttaaaatat gtgttagatt tatttttaat
     7501 tac
//
LOCUS       DAOTJA010000030         6973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_10375_length_6973_cov_6.105088, whole genome shotgun sequence.
ACCESSION   DAOTJA010000030 DAOTJA010000000
VERSION     DAOTJA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10375_length_6973_cov_6.105088"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..802)
                     /locus_tag="P5241_02725"
     CDS             complement(353..802)
                     /locus_tag="P5241_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32438.1"
                     /translation="MSIDNKYNGPDRNNSGKNIDKQGFDERIQTLKEKLAKFSGFKDL
                     ENISPEQLLSKTVEFYNALFHKNFPDDDFKISVKKTADNFFTIELNRNPNDIFEEIKN
                     KGANNDKSQYRNQQRKMCLIGTLLLDFEPTQDPLIITKQILNIIERF"
     gene            complement(895..3747)
                     /gene="uvrA"
                     /locus_tag="P5241_02730"
                     /pseudo
     CDS             complement(895..3747)
                     /gene="uvrA"
                     /locus_tag="P5241_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            complement(3927..4811)
                     /locus_tag="P5241_02735"
     CDS             complement(3927..4811)
                     /locus_tag="P5241_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV32439.1"
                     /translation="MLEITPDTILINNLELDHPDYYKNIQHLISVFQKYVNKLNKNQL
                     LIINKDDKNLKHIKTKSKLITFGIKNLKSDIIAQNIKINTHKHNQTFDVWAFGKKFGS
                     FEIELLGLINVYNTLAAISVAITWGVNSQDIKTALKKFPGIWRRLEKLGKYKGAIIFS
                     DYGHHPTAIKATLQAIKTFYPGHRIILAFQPHEHTRTKVLFKNFVKCFDDADVLLLEE
                     IYTVSGREHKKEIQSISSLDLVNAIKKRNKQIQVMYVKDNDVMLNNLNQVIRSGDIVL
                     IMGAGNIYNIISKLKLQN"
     gene            complement(4938..5333)
                     /locus_tag="P5241_02740"
     CDS             complement(4938..5333)
                     /locus_tag="P5241_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRV32440.1"
                     /translation="MNKQPKYLNNIKKVYFSGIGGIGVSALAKMMLSMHKKVFGSDIA
                     RSEITDKLQKQGATIVFKQVPENITKDIDLFIYSPAEDSNHPERKQAKKLKIKQYSYS
                     EFLGLISKDYNTISICGTHGKSTTTALTG"
     gene            complement(5761..6123)
                     /gene="rmuC"
                     /locus_tag="P5241_02745"
                     /pseudo
     CDS             complement(5761..6123)
                     /gene="rmuC"
                     /locus_tag="P5241_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
     gene            complement(6139..6486)
                     /locus_tag="P5241_02750"
     CDS             complement(6139..6486)
                     /locus_tag="P5241_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32441.1"
                     /translation="MSISIFLIILIVINICVVGFLLYKLLTITKNKNEVDKSQNDIIH
                     DLERRITDLLSMQLNEVRGSLDFNNKNIHEQVNNFTKETTEVKDFLKQVNERIKDMSN
                     FQDLFKTPKLRGQ"
BASE COUNT         2358 a         1135 c          903 g         2577 t
ORIGIN      
        1 taataatgcc actagcagtt ttgttatctt cttttaatgg ctcaacaaaa acataattgt
       61 ttaatggttg aatatccata atttctacat ttattatttt tgttttttaa tattagcact
      121 cttgaccaga aagtgctaaa caaatttata ttagcaaatt tttaaatttt ttgcaagccc
      181 catataaaaa ttgtgcttac gcacaatttt ccaatagtta ataattcgta gtaaaaacta
      241 aaaacaagtt agaagtaaga aattagaagt ttagaaaaat aaaaaaccac ataatcaagg
      301 tattagttta tcaatatatc ttacaaagtt actgccctaa tgtttctttt tgtcagaatc
      361 tttcaattat atttaaaatt tgttttgtta taatcaaagg atcctgtgtt ggttcaaaat
      421 ctaataaaag tgtacctatt aggcacattt ttctttgttg gtttctatat tgagacttat
      481 cattattggc acctttattt tttatttctt caaaaatatc attggggttc ctatttaatt
      541 ctatggtaaa aaaattatct gctgtttttt tgacagaaat tttaaaatca tcatcgggaa
      601 aatttttatg aaataatgca ttataaaatt caactgtttt ggaaagcaat tgctcggggc
      661 taatattttc taaatctttg aaaccagaga atttggctaa tttttctttg agcgtctgta
      721 ttctttcatc aaaaccctgt ttatcaatat ttttgccaga attatttcga tctggcccat
      781 tatatttatt atctattgac atattaaata aattaagttt ttataatgta taatataagt
      841 ataacaaaaa atacaaataa aagcaaggcc ctgtaggaaa cttattttgt aaatttattt
      901 taaaaactta gcagtgtaag attgcttatt tttctttaaa ttttcaggcg taccttcaaa
      961 aacaatttta cctccttggt cgcctccttc tggtcctaat tcaataactc agtcacagga
     1021 tttaattaca tctatttcgt gttctataat aatcacagta ttacccttag cgactaataa
     1081 atttaaaaca tttactaatt tttttatatc atcaaaatgt aatccagtag ttggttcatc
     1141 taaaatatac agagtatttc ctgtgtcacg tttagacaat tcatcagcta atttaactct
     1201 ctgtgcctcg ccacctgata atgtagtggc tggttgtcct aatttgatat atccaagacc
     1261 aacttgctct aataaatcta attttgatac tatagaggga atatttttaa aaaagttttt
     1321 agcttcagac acactcattt ccaaaacttc gctaatattt ttcccattat attctacttg
     1381 taaagtttct gaattaaaac gagtaccatg gcattcttca caagggacat aaatatcagg
     1441 cagtaaatac atttcaactt tttgaaaacc ctgtccctca caaacttcac acctgccacc
     1501 cttaacatta aaactaaacc taccttgatc atatccacga atacgtgcga gctctgcttg
     1561 agaaaaaata tatctaatct gatcaaacac gcctgtataa gtagcaggat ttgaccttgg
     1621 cgtacgacca attggagatt gatcaaccat aattatctta tcaatatttt ctaatccttt
     1681 tatatttttg taaccattag aaatatcgtc attatctaaa ttggaatacc caattttgcc
     1741 aatagaacga aaataattta taataccttt gtataatacc tcgataatca atgtagattt
     1801 tccagaacca gacacaccag taataccaat cattttacct aaaggaaatg atactgttat
     1861 attttttaaa ttaaatttat tggcatgatt aatggtaata aacttatcag caccacgatt
     1921 tgcagtttta tttgacaaat taacctttaa tttacctgat aaaaattgcc cagttaaggt
     1981 attggaatgt aataaatctt tgggagtgcc ttcaaaaaca actcttccgc cgtttttgcc
     2041 agctcctggg ccaatttcta ttatccaatc tgctgataaa atagtttggg ggtcgtgctc
     2101 tacgactata attgtgtttc ccagatcttt gagctgttta agattgttta ttaaacgttc
     2161 ctgatctttg ggatgtaatc ccacagaagg ctcatccaaa acataaataa taccagacaa
     2221 tcccgaacta atttgtgtag ccaatctaat tctttgtgct tcaccaccag acaatgtagt
     2281 tgattctctc gataaagtta aatattctac actaatatca tttaaaaaat tcaaacgagc
     2341 aataatttct ttcaaaagag gaagggctat tgttttttct ttcaaattta atttgttttt
     2401 taaatcttca aaatatttta aggcatcttt gatagtaaaa tctaaaatat cagctatatt
     2461 tttattatca atcaaaacac ttagagcttc ttgttttaat ctcttgccat tgcaggcctt
     2521 acaaatagaa tggtgcatat atttctcaat ttcttgttta gcaaaatccg atgtagattc
     2581 acgtcatcga cgttctaaat taggaataac accttcaaat ttatcatctc cgtataaaat
     2641 tatatttaaa attttatcag gcaattgtga aactggagcg tttatatcaa accccaatct
     2701 atcggcaagc ttttctaatt cataaaaata atacccttgt ctaccaacac gatgagaaac
     2761 tcttactcaa ggaattattg ctccttcggc aatacttaaa gttttgtttg gtataaccaa
     2821 ctctggatca acagaattta tagtaccaag acccatacat acaggacaag caccataggg
     2881 attattaaaa gaaaataatc gcggctcgat tttgggtaaa ctaaaaccac atttaggaca
     2941 agcaaataat tcactaaata ccaaagattg acttttggag ttttgaaaca taacataaat
     3001 aaccccctta ccaattttga gtcccaattc aacagaatca gagatacgag atttttctaa
     3061 atttttatca attataatat catcaataat tacttctaaa tcatgttttt tctgtttatc
     3121 aatttcttga ttcattagct cttctgtacg tcaaatagtt ttatcaaaac gcaccctcgg
     3181 ataacccaat ctataaacct cttctaacat acccttatgt tctccttttt tgccttgaac
     3241 tattggagcc aaaatataaa aatgggatcc gcgaggtaat gaacttattt gttttgatat
     3301 ttgactcgca gtttgacgag taatttgaat accgcatttg ggacaaacag ggacacctat
     3361 tcgagcaaat aacactctta aataatcata gatctctgtt atggtagcta cagtggatct
     3421 tggatttttc attaaagatc tttgatcaat agaaattgcc ggggataaat tctctatttt
     3481 ttctaaatct ggtttttctt taatgcctaa aaattgtcgt gcataagatg ataatgactc
     3541 aacatatcgt ctctcggctt cagcataaat agtatcaaaa gcaagggacg ttttaccaga
     3601 accagaaaca ccacaaaaca cgaccaattt gtttctcggt attttaacat ttatattttg
     3661 taaattgtgt actttggcac catagatatt aatagaatct tgcaaaagct tttgtttttt
     3721 tgctatagat ttagaatttt tagtcattgt tatattataa aattactata atattttatc
     3781 aatctatata ttatatcata aaacgctaca aaattcaata aaattatata tcaaaataat
     3841 atatacaatg catacacgcc atcaattgat aattagaaaa aacaaacata ttcaaatact
     3901 tagtataaga ataagttagt tttattttag ttttgaagtt ttaactttga aataatatta
     3961 taaatattcc cagcacccat aatcaaaaca atatcacctg acctaataac ttgatttaaa
     4021 ttattaagca ttacgtcatt gtctttgaca tacataactt gtatttgttt attacgtttt
     4081 tttattgcgt ttaccaaatc aagagagctt atagattgta tttctttttt atgctctcta
     4141 ccagatacag tatatatttc ttctaataac aaaacatcag catcatcaaa acattttaca
     4201 aagtttttaa ataaaacttt ggttctggta tgttcgtgtg gttgaaaagc caaaattatt
     4261 ctgtgcccag gataaaatgt tttaatagct tgcaaggtgg cttttatggc tgttggatga
     4321 tgtccataat cagaaaatat aatagctcct ttgtatttac ccaatttttc taaacgtcgc
     4381 catataccag gaaatttttt caaagctgtt ttgatatctt gactatttac tccccaagta
     4441 atagctactg aaatcgccgc caaagtattg taaacattta tcagtcctag taattctatt
     4501 tcaaaactac caaacttttt accaaaagcc caaacatcga atgtttgatt atgtttatgt
     4561 gtattgattt taatattttg agcaataata tcagatttta aatttttaat accaaaagta
     4621 ataagttttg atttggtttt gatgtgtttt aaatttttat catctttgtt tataattaac
     4681 aattgatttt tatttaactt gttaacatat ttttgaaaca cagagattaa atgctgaata
     4741 tttttataat aatcaggatg atctaattct aaattattaa ttaaaattgt gtcaggggta
     4801 atttctaaca tatgggccct tcattcacag gattcaacta caaacacatc tgaaaatccc
     4861 attctaaaat tatcatctca aaacttcatt ttggagccaa ctataaccat agggtctata
     4921 ttacctgctt ctaataatca acctgtcaaa gcagtagtag tacttttgcc atgagtacca
     4981 caaatagaaa tagtattgta atctttggag attaaaccca aaaattccga ataagaatat
     5041 tgttttattt ttaatttttt agcttgtttc ctttcaggat gattagaatc ttcggctgga
     5101 gaataaataa acaaatcaat atctttggtg atattttccg gaacttgctt aaaaacaatt
     5161 gttgctcctt gtttttgtaa tttatctgtt atttcagaac gagcaatatc tgaaccaaat
     5221 acttttttgt gcattgagag catcattttt gccaaagcac ttactcctat gcccccaata
     5281 ccagaaaaat atactttttt aatattgttt aaatattttg gttgtttgtt catattactt
     5341 taatgttaaa ttttaaatat tttcatattt tctacaacat attcggatat ataaatactt
     5401 agtattacaa tatgccagct ttaatattta tatatcagaa tcagaatcag acgaattgtt
     5461 agataaattt agctcctcaa catcattatt gccactttta aaatcaataa gcttggctac
     5521 tcgggtggtc attttattca ttctatcttc tgtctcttca taggctgatt tggcattgga
     5581 aatatgattg cctaattttc taaaattatc tgataattta gccgcatctt ttataataac
     5641 atttaatttt ttaagaatta aatgcgtttc ttctgataat ttagtatctt tgaatcaatg
     5701 ttcgataatt cttaatgtca aaaataaagt attaggagac gtaataacaa tctttttaga
     5761 tcaagcatat tccattatat tatattcttt gtctattgta tttattagct cataataaat
     5821 tgcttcggct ggtatataca ttaaggcata gtctactgtg ttctcgttag gcaaaatata
     5881 tttagtggct atttcatcaa tttttttctt gacatcggat acaaatattt tttgatattc
     5941 ttttttatca ttttcgtttt cagtttccat cattttgata aaattttcag aggggaattt
     6001 agcatcaata ggcaaaatct ggccatctgg taatttaaaa atggcatcta cttggttacc
     6061 agtagaaaat aaatactgct cttgccataa atctttggga taatattgtg ataataaatg
     6121 atataattgt gcctcacctc actgaccacg cagcttaggg gttttaaata aatcttgaaa
     6181 attagacata tctttgatac gctcattaac ttgttttaag aaatccttga cttctgtggt
     6241 ttctttggta aaattattta cttgctcatg aatattttta ttattaaaat ccaaagaccc
     6301 cctaacttca ttaagttgca tacttagcaa atccgtaata cgacgttcga gatcatgaat
     6361 aatatcattc tgcgatttat ctacttcatt tttgtttttt gttattgtta ataatttata
     6421 taataaaaaa ccaacaacac agatatttat aacaattaat ataataagaa aaatagagat
     6481 agacataata ataattttaa gtttttaaat attatgtgaa ataagatttg agtctacgtt
     6541 taatttaaga tatattatac cataatgggt tttaaaatac ctttctctat gtctggcatc
     6601 gaattcgttt aaacatgcct cataatatat caatttgata gactttcagt ttttggtagt
     6661 tttaatctta tgtttcaaat ttaggaaatt tagataatat cggatttaaa aaaatagtta
     6721 aatcctccaa ttgctgaaaa acaataagat ctttgatcat aatagtatat taaaaattca
     6781 attaaggtaa ggggtttgta atccgctaac gcggattaca aaaagagttt aagatttcag
     6841 attgctacgt aacagttacc tcgcgcaacg aaacccaaca ttgttgttct gagtagtagt
     6901 ggatcaagcc aaagtcagca caaaagcacc cgcattagaa ccatcgcgcc aactgccacc
     6961 acgaagcaac acc
//
LOCUS       DAOTJA010000031         6857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_10566_length_6857_cov_5.762423, whole genome shotgun sequence.
ACCESSION   DAOTJA010000031 DAOTJA010000000
VERSION     DAOTJA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10566_length_6857_cov_5.762423"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..305)
                     /locus_tag="P5241_02755"
     CDS             complement(156..305)
                     /locus_tag="P5241_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32442.1"
                     /translation="MTENLMQHIVNLCKRRGFITLSSEPYGGFGSCYDYGPLGVELKN
                     NIKQN"
     gene            complement(334..471)
                     /locus_tag="P5241_02760"
     CDS             complement(334..471)
                     /locus_tag="P5241_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32443.1"
                     /translation="MTLLNESVLTAEDAFTQNTIQTKSNGFFSNTLTDKTVGPMDGIV
                     R"
     gene            complement(718..1533)
                     /locus_tag="P5241_02765"
     CDS             complement(718..1533)
                     /locus_tag="P5241_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32444.1"
                     /translation="MDFSNLRLNMTYPEGYILNESSITPLESNNIFDLGDIGPNAQGF
                     ITLNGYITGQGGENKKFNTTIGTYNDATKTFLPITTLDSFASLVANPLMLGVTINDEV
                     AGAVNAGDMLNVSIKYQNNYQVGIQSLVLKVVLEGDMFDYTTLSTNKGYFSAKNRTIT
                     WTESQIPQLAVLNPGETGIINFNIKLKDNFVIKNYNDKNFIVEVKASLESKVPLTGME
                     QNIYAKTSSSAMAKLNTKVDLITTIFYRDLNSNITNCGTLPLKVDTPTCFTVH"
     gene            complement(1549..1962)
                     /locus_tag="P5241_02770"
     CDS             complement(1549..1962)
                     /locus_tag="P5241_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32445.1"
                     /translation="MEFSVIAPKEIKAGEIQTITFAYKNGMRVNLENAEIIISLPDNI
                     YAPNEPNNKIIHINVGIIEKNTSATKTLELIAIGEKNTIVSFDTSFRYKPVTLTSVFE
                     KKIKTDMGIYGSAIGLNIDMPTQILPDTNFDIKIS"
     gene            complement(2049..2264)
                     /locus_tag="P5241_02775"
     CDS             complement(2049..2264)
                     /locus_tag="P5241_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32446.1"
                     /translation="MIKHLGFKPLLKETTTNKANINKQTLRSLQILFQENINDIIKNG
                     LAEPILNQKNNIQQYLNQLLCKIIQSY"
     gene            3309..3665
                     /locus_tag="P5241_02780"
     CDS             3309..3665
                     /locus_tag="P5241_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32447.1"
                     /translation="MNIASNKLFLSQNQDPHIPVPKIIQVVVTAYSSEEAQTDEDPFT
                     TASGVQVTDGIIASNDLSFGTRVQFPEIYGDKIFVVADRMNERYTDKNHIDIWMEETH
                     KAIHFGVQHTYAVILD"
     gene            complement(4285..4692)
                     /locus_tag="P5241_02785"
     CDS             complement(4285..4692)
                     /locus_tag="P5241_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32448.1"
                     /translation="MCDQYNTDDKLIPTDISTETYGPCYTYEYIIKNNICYQLPQEFL
                     DCKASKYQKLDACDQYLEDVTAKLAKDENGYPFEQICEIKINIPTNSLKTNNQPTQSL
                     QQATLVKQPHKNIFNRIIDFFKYTFLQIFSKSY"
     gene            complement(4828..5256)
                     /locus_tag="P5241_02790"
     CDS             complement(4828..5256)
                     /locus_tag="P5241_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32449.1"
                     /translation="MKITTKILIVLTLCCSFAGMIKADSGPLTNQTTFYFQQNGQPIT
                     QPVHFNIKCYGVSIYRQGEDIPDSQEGFTENKEKGIIESNKLLQISEMSETCLSYGCK
                     FNTSNIFRAYRKIMKYCTLEGNINGQKFVIDKFLDNDLSG"
     gene            complement(5332..5415)
                     /locus_tag="P5241_02795"
     tRNA            complement(5332..5415)
                     /locus_tag="P5241_02795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5379..5381),aa:Leu,seq:taa)
     gene            complement(5615..6316)
                     /locus_tag="P5241_02800"
     CDS             complement(5615..6316)
                     /locus_tag="P5241_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV32450.1"
                     /translation="MPSGPGEKQHLIQKGIKENKIHLSPNATDTNIFVNIESDDIVKQ
                     YNLNNKYVVGYAGLLGFAQNPIIIIETARELQNYKNIIFFIVGEGGMKIEMEQLADKY
                     KLNNVIFVGEKPRKEIPYYINTFSCCLIPYKNTSLFTRTIPSKLFDYLAAGKPVIINL
                     EGYASQIIKTANAGLVAQPDNSIDLAKQILYLHNNIEIAKQMGINGRKYVEQYYDREK
                     LLKELALFLINIVQK"
     gene            complement(6416..6589)
                     /locus_tag="P5241_02805"
     CDS             complement(6416..6589)
                     /locus_tag="P5241_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32451.1"
                     /translation="MRILSYLSFVLSASIASLKIPKHDIIIVASPPLFVPLIGITVKQ
                     KQHTPLVLDITDI"
     gene            complement(6668..>6857)
                     /locus_tag="P5241_02810"
     CDS             complement(6668..>6857)
                     /locus_tag="P5241_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HRV32452.1"
                     /translation="KLSICFMKILLITQNFPPDIGGASNRTSGIAHYLSTFGHQVTVL
                     CANPIYPRGKIFPGYKNK"
BASE COUNT         2223 a         1020 c          955 g         2659 t
ORIGIN      
        1 ctcaatttaa atcgtttatg acattgtttg cactcgacta actcatcagc aaaaccagct
       61 gataaatggc cagaagcttc tcaaactttg ggcgacatta aaatagcact atcaacccct
      121 acaatattat cataacctct tgtcatagtt gttcatcagt tttgtttgat attgtttttt
      181 aattctactc ccaaaggacc ataatcataa caactgccaa aaccaccata aggttctgag
      241 gaaagagtta taaaacctct ccttttacac aaatttacaa tatgttgcat taaattttct
      301 gtcatatata taatttaata ttctctattt attttatctt acaatcccat ccattggacc
      361 aactgttttg tctgttagag tattcgaaaa gaatccattt gatttagttt gtatagtatt
      421 ttgagtaaat gcatcctcag ctgttaaaac cgattcattt agcaatgtca tattgctatt
      481 ttgttgctgt attgttgggg tggctgatat ttggaatatt aattcataag ctttagaaat
      541 tatgcccgaa cctgatgcaa tattagaaag cgttcaagtt atttgtcgtg atataggatc
      601 ataacttggc cccacaccgc cataattacc tgaatattta ccagtatatt ctacacccat
      661 tggtaaggtt gttgtaattt tgacattatt taaattattg gtataatttt ttattaatca
      721 gtgcacagta aaacaagttg gtgtgtctac ttttaatggt aaagtaccac aattagttat
      781 attactattc aaatctctat aaaatattgt agttataagg tcaactttgg tatttaattt
      841 tgccattgca ctgctacttg ttttggcgta aatattttgt tccatacctg ttaaaggtac
      901 tttactttcc aaagatgctt ttacttctac tataaaattt ttatcattat aatttttaat
      961 aacaaaatta tcttttaact taatattaaa attgattatg ccagtttccc cgggattaag
     1021 cactgccaac tgaggaattt ggctttctgt ccaagtaatt gttctatttt tggcactaaa
     1081 atatcctttg tttgtagata aagttgtata gtcaaacata tcgccttcca atactacttt
     1141 taataccaga ctttgaattc ctacttggta attattttga tattttatcg atacatttag
     1201 catatcccct gcatttacag cgccagctac ctcgtcattt atagtaacac caagcattag
     1261 cggattggct actaatgatg caaaactatc caaagtggta ataggcagga atgtttttgt
     1321 ggcatcatta taggtgccaa tagttgtatt aaatttttta ttttctccac cttgtccagt
     1381 aatatatccg tttaaggtta taaaaccttg ggcatttgga ccaatatcac ccaaatcaaa
     1441 aatattatta ctctccaaag gcgttatact actttcattt aaaatatatc cttcgggata
     1501 tgtcatattt aatcgtaagt tagaaaaatc catatttgat tggttttctc aagatatttt
     1561 tatatcaaaa tttgtatctg gcaatatttg tgttggcata tcaatattta aaccaatcgc
     1621 tgaaccatag atacccatat cagttttgat tttcttttca aatactgatg tcaaagttac
     1681 tggtttatat ctaaaagatg tatcaaacga cacaatagta tttttctcgc ctatagctat
     1741 taattctaag gtcttggttg cactcgtatt tttttcaata atacctacat ttatatgaat
     1801 aattttatta tttggttcat tcggagcata tatgttgtct ggtaaactaa taattatttc
     1861 ggcgttttct aaatttactc gcataccatt tttataagca aaagtaatag tttgtatttc
     1921 gccagcctta atttctttgg gtgctattac tgaaaattcc acacctcctt ggcgtagtaa
     1981 tcatcatact ccatatccaa ttgccaatat tgctatggtc gatatcattc ctatcaaaaa
     2041 tgatttattt aatagctttg aattattttg cataataatt gatttaaata ttgttgtata
     2101 ttattttttt gatttaaaat tggttcggcc aaaccatttt ttataatatc atttatattt
     2161 tcttgaaata aaatttgtaa tgatcttaat gtctgtttgt ttatattggc tttgttagtt
     2221 gttgtttctt tgagtaatgg cttaaaccct aaatgtttta tcaattgcgc ttcaaatcag
     2281 tattcaatta gcaaagatat tgtatctttg tcttttgttt tttctaatat atccaaagtt
     2341 tgttttaaaa tatttcaaag atccaaatca gtttcaggaa ataaaatata ttgatcaaca
     2401 agttttaaaa tatgttggat agtttgcaag gcattacaac tggtgcgtat ttttgaaaaa
     2461 ttatttttta ttagagcacc tgttaatgta ttattataac cagttataaa attgatatta
     2521 gttaaacagg gtggctctat ttgcccctga agcttagcat tgggttttct aatacctttg
     2581 gcgattacta atattttacc aaatttttta gtaaaaaagc aatactctct atcagattcc
     2641 ccaatattct tttttcataa tgttatagct tgggttagat acataaattg tatattatac
     2701 tttaataata gcacaaacac cataaaaaat caaatattaa agtccaaaac cacagatata
     2761 aaaataacta atatatattt ttgtattcag tatttaaaaa atatttgaat tattagataa
     2821 ttatagcctt ggtattcttt tgtggcaaag atttatagga catttttatt gccaattgga
     2881 cggcttatta agtaatttaa tctctaagtg aaatattgct tatttttatt aacattttac
     2941 ctctatttct taattatttt acttctaatg aatcttttaa tttgtcaaag tgtagcagta
     3001 aagaatagaa cttgctcttc tctggatctt gacttttttc taataatatg gtagtattaa
     3061 ggtattataa ggatatatta tcataatata tcattataat agcatagcta gcaaaagctc
     3121 tcacaaattg agagtttttg ttcttaaaaa tgtgtataaa cagagaatgt ctcgtaaaat
     3181 ataggttttt cgagcaacta aaattaaaac attttgtttt gattttagta agtatggggc
     3241 tatttatagg ccttagctga cctatattaa ctattaatgc tatttctttt gatcccgcta
     3301 atgatgctgt gaatatagct tctaataagc tttttctttc acaaaatcaa gatcctcata
     3361 ttcctgttcc taaaataatt caagtggtcg ttactgctta ttcatctgaa gaagcacaga
     3421 ctgatgagga tccttttact acagcatcag gggttcaagt tacagatggc attattgcgt
     3481 ctaatgattt atcgtttggc accagagtac aattccccga aatatatggt gataaaattt
     3541 ttgtagtagc ggatcgtatg aacgaacgct atacagataa aaatcatata gatatttgga
     3601 tggaagaaac acacaaagct atccattttg gtgtccaaca tacttatgca gttatattag
     3661 attaataaaa taataaatac gcatcgttca attttgaacg atgcgtattt tgtttttata
     3721 taattttaat tttgttataa agaattttaa aaaatctcaa ccgaacttct acatagttat
     3781 ctatggtaaa aattctatct aacacttttt ttatttcttt cttatatttt gtagtattcc
     3841 aatgtaccaa gagtactgtt tgaattgcta caattggtat ttcccattat tgaatagagg
     3901 cagacttata tgttttaaaa taaacataac acttaaatta tctgaacaca attaggccag
     3961 ctaaatatga tgcaatattg gcaattatag ataaaatagt agcgttttta agatttattc
     4021 taaatgcttt tgtgaatatt attgtttcaa ccaaaatgat caaaatttca ccaatcaaaa
     4081 tataattatg tgctgagata aatagaggga aaacaaacca taaataaggt aaggaaaata
     4141 aatttacaaa aacaccccaa tataacattt tcttttttga taattttatg tttagaacaa
     4201 atttcgttaa aagaaataat attgggattt ccacaattaa tgttagcaag agcgagagaa
     4261 aaaagaaaaa tccgtaagac ataactaata agatttacta aatatttgta aaaatgtata
     4321 cttgaaaaaa tcaattattc gattgaaaat atttttgtga ggttgtttaa ccaaggttgc
     4381 ttgttgcaga ctttgagttg gttgattgtt tgtttttaaa ctattagtag gaatattaat
     4441 ttttatttca caaatttgct caaaggggta tccattttca tctttcgcaa gtttagcagt
     4501 cacatcttct aaatattgat cacaagcgtc taatttttga tatttactag ctttacaatc
     4561 caaaaattct tgcggaagtt gatagcaaat attgtttttt attatatatt cataggtata
     4621 acaagggcca taagtttcgg tagagatatc tgttgggatt agtttgtcat ccgtgttgta
     4681 ttgatcgcac acaaaaatac ctttttcttc cccatttcct tgcggatggt attctttgta
     4741 aacatttttt ctacattcct ggtattgggg agttttgttg taatacttat catctgatac
     4801 aattatataa tcgttggtac gacagtttca tccacttaga tcattatcta aaaatttatc
     4861 tattacgaat ttttgtccat taatgtttcc ttctaaagtg caatatttca taatttttct
     4921 gtaagcccta aaaatattag atgtattgaa tttacagcca taagataaac aggtttctga
     4981 catttcggaa atttgtaaca atttgttact ttcaataata cctttctcct tgttttcagt
     5041 aaaaccttct tgactatcag gtatatcttc cccttgtcga tagatagata ctccatagca
     5101 cttaatatta aaatgtactg gctgagttat aggttggcca ttttgctgaa aataaaaagt
     5161 tgtttgattg gttaaaggac ccgaatctgc ttttatcatt ccagcaaaag agcaacataa
     5221 tgttagaacg attaaaattt tggttgttat tttcatagct tattatattt ttatagctac
     5281 taactattga ctacaaatta cgaagaaaat aaacaaaagc acaatttttt gtgcctaggg
     5341 tgggactcga acccacacga tattgctatc aagggatttt aagtccccat tgtctaccaa
     5401 ttccaacacc taggcatata gcatatatgt ataatattaa tttttttgtg tgtcgtatat
     5461 tttatatatt aacacaaaat ataaaaaaaa tcaatatatt tttgacaaaa tataaaaaaa
     5521 atcaatatat ttttgacaaa atataaaaaa atcaatatta taggattcaa aatctttggt
     5581 tattatttta aaccaagaat ttttagttta ttatttattt ttgtactatg tttattaaaa
     5641 ataatgccaa ttcttttaat agtttttctc tatcataata ttgttcaaca tattttctgc
     5701 catttatccc catttgtttt gctatctcaa tattattatg taaatataat atttgttttg
     5761 ccaaatctat gctattgtct ggttgcgcta ctaaaccagc attggctgtt ttaattattt
     5821 gtgacgcata tccttcaaga tttataatga ctggttttcc agctgctaaa tagtcgaata
     5881 atttactggg tatagttcga gtgaataaag aagtgttttt atatggtatt aaacagcaag
     5941 aaaaagtatt tatgtaatat ggtatttcct ttcgtggttt ttcgccaaca aaaattacat
     6001 tatttaattt atatttatca gccagttgtt ccatttctat tttcatacca ccctctccta
     6061 caataaaaaa aatgatattt ttataattct gtaattctcg ggctgtctct attattatta
     6121 tgggattttg agcaaacccc aataaccctg catatcctac aacatattta ttatttaaat
     6181 tatattgttt tactatatca tcagactcaa tatttacaaa tatattggta tctgtagcat
     6241 tagggcttaa gtgaattttg ttttctttta tacctttctg tattagatgt tgtttttctc
     6301 ctggtcctga aggcacaaaa taatcacaat gcttgtaaat aaataattca aaatgctcgg
     6361 ctaatttaaa tattcatcct tttttcataa aaccagtggc gtttgctgat tctggtcaaa
     6421 tatctgttat atctaatact agtggtgtat gctgtttttg ttttactgtt atacctatta
     6481 aaggcacaaa taatggcggg gaagcaacta ttataatatc atgcttaggt atttttaaac
     6541 tcgctatact agccgataaa acaaaactta aatatgacag tattctcatt atagcaggcg
     6601 atttgggttt gactggtcaa attcatgttc tatacacaac atattcattg tttttgtctt
     6661 tttgtagtca tttattttta taacctggga aaattttgcc ccgtggatat attggattag
     6721 cacatagcac tgtaacttga tgtccgaaag tcgagagata atgtgcaatg ccactagttc
     6781 tattagaggc gccacctata tcaggaggaa aattttgtgt tataagtaat attttcataa
     6841 aacatattga taatttt
//
LOCUS       DAOTJA010000032         6764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_10723_length_6764_cov_3.818900, whole genome shotgun sequence.
ACCESSION   DAOTJA010000032 DAOTJA010000000
VERSION     DAOTJA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10723_length_6764_cov_3.818900"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="P5241_02815"
     CDS             complement(<1..890)
                     /locus_tag="P5241_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV32453.1"
                     /translation="MEINKVSIIRVAWVHNYDRSLNSASGVFMYQLYDGIKNSNSEIS
                     IDLINIGSIKNPLLFISKMIKYRKILRGYDIIHAQYGSGTGFFVSLFNKTKIISLRGS
                     DWYKSPANSIKSKLHIWLGCKLTRWSLSRFNHIIVMSERMKADVHSFAPNCKVIVIPD
                     GIDLNRFFPKQRSTNDKFRVLFSTVNKKNPVKRYDLALSAFNLFHCKYPNSELILMTG
                     VEPDRVNEFVNSVDVILLTSTHEGWPNIIKEGLACNIPFVSTDVSDLAKIADQTPTCY
                     VCDASPESLSAALEQAYFKR"
     gene            complement(872..2161)
                     /gene="wzy"
                     /locus_tag="P5241_02820"
     CDS             complement(872..2161)
                     /gene="wzy"
                     /locus_tag="P5241_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HRV32454.1"
                     /translation="MIYILLILAFVIIYYILDIFIKTEGFVKVVFLFILFAFLYYLGL
                     PIEMTLMGTSLGGYGFISDRVLIIITSMGVLSIAGFMLGFKISNYRFHDIKSFELVNS
                     RFLFFSLLLLFVFSSVILFVLFKDSLLSSISSYAGNVSETYNNPIYAYLKEIFISSTA
                     ILILMMTLKRGWYLLIAMLLSLTLIIFGIITSDKDPLLLSALAWGIYFFKFLSNHNLH
                     KVITYLMLLIILVVLIPALSLLFSLNRGGNINVTEFKNQIETNGIYKYFDATGPLVSL
                     VETLEEPNVDFEYGKTYFWGFVGWIPKSVWTNRPLDLAEKYSKEKIKNWQPGQGLGYS
                     LLTEAYINFGVAGALIQYCLIGLLIGLIGRLHQWLFREPIAPFIFFIWMSYNIAIMHR
                     GPFNLPSTYLRFVLPILFCYYSLYFSSIIWKKWKSIK"
     gene            complement(2158..3432)
                     /locus_tag="P5241_02825"
     CDS             complement(2158..3432)
                     /locus_tag="P5241_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32455.1"
                     /translation="MLPYRYFSYLDLKIVKQYAIAFGFSVIGALINFYFFRQVFHHIG
                     EESFYYYAYSRRIISFASPVLLLGLGVSLPRAIGLYKNDTVKQQRLFLLTVLIISIIS
                     TLWILVSFFINTGITKLLWGEESEITLKLSLVLSFFLFGTNVSAVIHSYYRGKINALM
                     SGIIELFVQSIIPLVSFIALSDLIQIYSAIAVSIIFFNIVIIYYMIRKFEVAIKHFIN
                     ISELKELIDYGIRRVPGDFFYSMITFLPAFLAGRFFGVEFAGVISFGLSLLTLFNLPA
                     TAISFVTLSRSAELLATSKPRLKNETYLLLSIAITYSFFIITFCYLFLDTLLKLFFDA
                     TFTKHFESIFTILLALPMLVVFTVLRSITDSAFKRSYNSIFMFIGLLVFILFAIFAKY
                     LSNSDYILYGNISVYGVLAVSSFIISRKIFQI"
     gene            complement(3454..4443)
                     /locus_tag="P5241_02830"
     CDS             complement(3454..4443)
                     /locus_tag="P5241_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV32456.1"
                     /translation="MMFLYILLLILSFLGVLIYRRIAIRSNIIDIPNERSSHTVPTPR
                     GAGVAVSAIWFITIIYLFLIEKKIPSNLFYALSGGLLLSLIGIIDDIWSISPKVRLIV
                     QIIASSIALFALGGLPKIDLGFYCIESFWLLTPIAFVMIIWFINLFNFLDGIDGYLGV
                     ETLFIFGFVGFFFSDNTAICLALIIVGFLFHNWPKAKVFMGDVGSTLIGYNVAVFALY
                     YQNSGKTSLIIWLILSSLFWFDATLTLYRRFRNKENLMKAHKKHAYQRIVQAGYSHQK
                     TLFYSIGINIIILGLVIMAIIYMKFSFFLLVLNVLLLYCIDRLIGINKPFPAE"
     gene            4576..5697
                     /locus_tag="P5241_02835"
     CDS             4576..5697
                     /locus_tag="P5241_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HRV32457.1"
                     /translation="MQTLQFGVERIPLKLIFYGENLDEVKVNYSNNITTSTSLDTLSC
                     SSNVCIFNVPEYAFTRYLNISLNYSDSLEIHKIEIHGNKNYITIKPRDFPNYLRYKQF
                     TYNTKNNRFYPTSDFDNNQTKRLDIRTIDLSNLIIDLYIKRIPQKTILLISIVITLIL
                     TILLYFISLKSEELKTASMIIVAIIIATSSIFSYIKFNQKNTIKENLYEADIFDIIKP
                     HTPNNLIYNGDFKYGLMFWGYDSDSTTHQLVDTPYGKGVKITRGDGDGGYWSLRYYGK
                     PITYHKNKTYVINFKYKIIKSVYKKVFNVGWWINRQNNEYRKVYDLNYKTKQLGNGWI
                     YLEAYYRFSEDQHNLICLLNSLKDNSEIIITDVGIYEKI"
     gene            complement(5717..6589)
                     /locus_tag="P5241_02840"
     CDS             complement(5717..6589)
                     /locus_tag="P5241_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV32458.1"
                     /translation="MRVVVTGASGFVGTNFIKSCKSLEIIELDLLKNKIEEISFLGVD
                     SVLHLAALVHQMNDIPEEQYFKINRDLAYSVAKKAKNNGVKHFILLSTVKVYGESHCN
                     NVIYDESSTCHPKDSYGRSKHEAEKLVKSLEDINFKVAIVRSPLVYGPGVKANMLNLI
                     KLVDKYKFLPFGCINNKRSMVYVNNLVELLKIIIEKKASGIFIAGDSKPLSTTEIIKY
                     IARGLNKNVYLFKTPILIQKILKILFPKYINRIYGSFVVTNKNTNDKLKFNPPYSSES
                     GFVEMVQWYIKKKN"
     gene            complement(6586..>6764)
                     /locus_tag="P5241_02845"
     CDS             complement(6586..>6764)
                     /locus_tag="P5241_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV32459.1"
                     /translation="TLYYPFVSVYHEYESGANKSFRLFIIFIISAIKYFNKWGWFFDN
                     ERIKINNTILNHLR"
BASE COUNT         2605 a         1081 c          896 g         2182 t
ORIGIN      
        1 tttcttttaa aatatgcttg ttcaagagca gctgataaag attctggtga ggcatcgcat
       61 acgtaacaag tcggagtttg atctgcaatc tttgctagat cactaacatc tgtacttaca
      121 aatggtatat tacaggctag tccctctttt attatatttg gccatccctc gtgtgttgat
      181 gttaacagga tcacatcaac actattgaca aattcattta ctctatcagg ttccacacct
      241 gtcatcaaaa tcaactcaga attaggatat ttacaatgaa atagattaaa cgccgataat
      301 gcaaggtcgt atcgctttac aggatttttt ttatttactg ttgaaaataa aactctaaac
      361 ttatcgttag ttgatcgttg ttttggaaaa aacctattta aatctatacc atcaggaatt
      421 acaataactt tacaatttgg tgcaaaacta tgtacatcgg ctttcatcct ctcagacata
      481 actatgatat gattaaatct tgacaggctc catcttgtaa gtttacaacc aagccaaatg
      541 tgaagtttac ttttaatact attggctggt gatttatacc aatcagatcc tctcaagctt
      601 attatctttg ttttgttaaa tagggataca aaaaaacctg ttccagaacc atattgagca
      661 tgaattatat cataacctct cagaattttt ctatacttaa tcatttttga aatgaataac
      721 aaaggatttt taattgagcc aatattaatc aagtcaatag aaatctcact attggaattt
      781 tttataccat catacaactg gtacataaaa acaccagaag cagaatttaa acttctatcg
      841 taattgtgaa cccaagcaac tctaattata cttactttat tgatttccat tttttccaaa
      901 tgatacttga aaagtataat gaataataac aaaataaaat tggcaaaaca aacctcaaat
      961 aggttgatgg aagattaaag ggccccctat gcattattgc aatgttatat gacatccaaa
     1021 taaagaaaat aaaaggcgca attggctctc taaacaacca ttgatgtaat ctccctatta
     1081 aacctataag taacccaata agacagtatt gaatcaatgc ccctgcaact ccaaaattta
     1141 tgtatgcttc agttaataaa ctatatccta ggccttgtcc cggttgccag tttttaattt
     1201 tttcttttga atacttttca gctaaatcca aaggtctgtt tgtccaaact gacttaggaa
     1261 tccaacccac aaaaccccaa aaataggttt tcccatactc aaaatctaca tttggttctt
     1321 caagtgtttc cactaaagaa acaagcgggc ctgtagcatc aaaatattta tatataccgt
     1381 ttgtttctat ctgattttta aattcagtaa cattaatatt accacctcta ttcaatgaga
     1441 acaatagact tagcgcaggt attaaaacca ctaaaattat aagtaacatt aaataggtaa
     1501 taactttatg taaattatga tttgaaagaa atttaaagaa ataaataccc catgctaaag
     1561 ctgataaaag caaaggatct ttatcgctag taataatacc aaaaataata agagtaagac
     1621 ttaacagcat tgcaattaat aaataccaac ctcgtttaag agtcatcatt agtattaaaa
     1681 tagcagtact acttataaat atctctttca agtatgcgta tatgggatta ttataggttt
     1741 cgctaacatt accggcataa gaactaatcg aacttaacaa tgaatcctta aataaaacaa
     1801 aaagaattac acttgaaaac acaaaaagta ataataatga aaaaaataga aatcgactat
     1861 taaccagctc aaatgacttg atatcatgaa atctatagtt tgatatctta aagccaagca
     1921 taaaaccagc aatagaaagc acccccattg aggtgataat tatcaaaacc ctatctgaaa
     1981 taaatccgta tcctcccaat gatgtaccca ttaaagtcat ttcgataggc aaacctaaat
     2041 agtaaagaaa tgcaaatagt ataaacaaaa aaacaacctt aacaaatcct tcagtcttaa
     2101 tgaatatgtc aagaatataa taaataataa caaatgccaa tattaacaat atgtatatca
     2161 tatttgaaat atttttcttg aaattatgaa gctcgataca gcaagtaccc cataaaccga
     2221 tatatttcca tacagtatat aatcggaatt gcttaaatat ttcgcaaaaa tagcaaaaag
     2281 gatgaaaact agtaaaccaa taaacatgaa tattgagtta tacgatcgct taaatgctga
     2341 atcggttata cttcttaaaa cagtaaaaac aacaagcata ggtaaagcca aaagtatagt
     2401 aaaaatggat tcgaaatgct ttgtgaatgt tgcatcaaaa aatagtttaa gtagagtatc
     2461 aagaaaaaga tagcaaaacg taataatgaa aaaagagtag gttattgcaa tagataataa
     2521 taaataagtt tcatttttca gtcttggttt ggatgtggct aaaagttctg cagatcgcga
     2581 caaagtaaca aatgaaatag cagtagcggg taaattaaaa agggtcaata gcgatagtcc
     2641 aaaactaatt actccggcaa attcaacacc aaagaatctg cctgccaaaa atgcaggtag
     2701 aaaagtgatc attgaataaa aaaaatcacc aggtacacga cgaattccat agtcaattaa
     2761 ttcttttaac tcagaaatat ttataaagtg ctttattgca acctcaaatt ttctgatcat
     2821 ataataaatt atcactatat taaaaaaaat gatacttacg gctattgctg aatatatctg
     2881 aatcaaatcg ctcaatgcaa taaatgatac cagtggaatt atagattgaa cgaagagttc
     2941 aattatccct gacattaatg catttatttt tcccctgtag taagaatgaa taacagcact
     3001 tacatttgta ccaaataaga aaaaagaaag cactaaactt aacttcaatg ttatttcact
     3061 ctcttcaccc caaagcaatt ttgtgatacc ggtatttatg aaaaaactta ctaatatcca
     3121 taatgtagaa ataattgaaa taattaatac tgtcaatagg aataaacgtt gttgcttaac
     3181 agtatcattt ttataaagac caattgctct tggtagtgaa acgcctaacc ctaataacaa
     3241 tactggagat gcaaatgata taatgcgtcg tgagtatgcg taatagtaaa aagactcctc
     3301 tcctatgtgg tgaaatacct gtcgaaaaaa atagaaatta attaatgctc caataactga
     3361 aaaaccaaaa gcaattgcat actgcttaac aatttttaga tcgagatatg aaaaatacct
     3421 atatggcaac atctaacagt atgctgttat attctactca gcaggaaatg gtttattaat
     3481 ccctattaat ctatctatgc aatatagtag cagtacattt aaaacaagta agaaaaatga
     3541 aaatttcata taaattatag ccattataac caacccaagt ataataatat ttatacctat
     3601 agagtagaac aaggtctttt gatgactata acctgcctga actatacgtt gataagcatg
     3661 ctttttatgt gctttcatta gattttcttt atttctaaat cttctataca acgttaaagt
     3721 tgcatcgaac cagaagagag aagataatat tagccaaata ataagtgatg ttttacctga
     3781 attttgatag tatagtgcaa atacagcaac attataccca attagtgtac ttcctacatc
     3841 gcccataaat actttagcct taggccagtt atggaacaaa aaacctacaa taattaatgc
     3901 caagcatatt gcagtattat cagaaaagaa gaaacctaca aatccgaaaa taaaaagggt
     3961 ttcaacacct aaataaccat caattccatc cagaaaatta aaaaggttaa taaaccaaat
     4021 aatcattaca aaagctatag gtgttaaaag ccaaaaactt tctatacaat aaaatcctag
     4081 gtcaatttta ggcaatcctc caagagcaaa caatgcaatg ctcgaagcta taatctgtac
     4141 aatcaatcga acttttggtg aaatactcca aatatcatca attattccta ttaacgataa
     4201 gagtaaacca ccactaagag cataaaacaa attagaaggg attttttttt caataagaaa
     4261 taagtaaatt attgtaatga accaaatagc cgatacggca acaccagcac ctctaggtgt
     4321 aggaactgtg tgagaacttc gttcatttgg aatatcaata atattagaac gtatcgcaat
     4381 acgacgatag atcaatacac ccaaaaaaga taatattaat agtaaaatat ataaaaacat
     4441 cattttaata ttttagaata aaattgtaat ttcgatgtaa atatgttact tttgaaaaaa
     4501 tttataatta tgttcagagg caaaactacc tacattacat ttgcaataac ttgttttctt
     4561 ctttgtattg cattattgca gactcttcag tttggagtag aacgtatacc tctcaaactt
     4621 attttttatg gtgaaaacct tgatgaggtt aaagtcaatt atagcaataa cattacaacc
     4681 agtacgtcat tagatacatt aagttgttca agtaatgttt gtatatttaa tgtaccagaa
     4741 tatgcattta caagatacct aaatatttct cttaattaca gcgattcact tgaaattcac
     4801 aaaattgaaa tacatggaaa taagaattat attacaataa agcccagaga ttttccaaat
     4861 tatctaagat ataagcagtt cacctataac acaaagaata atagatttta tccaacaagt
     4921 gattttgata ataatcaaac aaaaaggctt gacattcgaa ccattgacct ttcaaatctt
     4981 attatagatc tctatattaa aagaattcct caaaaaacaa ttctattaat cagcattgtt
     5041 attacgttaa ttttaactat attattatac tttatttcat taaaaagtga agaacttaaa
     5101 actgcttcta tgattattgt agcaattatt attgctactt cttctatttt ctcttacata
     5161 aaatttaatc aaaaaaatac aataaaagag aatttatatg aagcagacat atttgatata
     5221 attaaacctc acacacccaa taaccttata tacaatgggg actttaaata tgggttaatg
     5281 ttttggggat atgattcaga ttctactact catcaactag ttgacactcc atatgggaaa
     5341 ggagtgaaga taactagagg agatggtgac ggcggttatt ggtcattaag gtactacgga
     5401 aaacctataa cataccataa aaacaaaaca tatgttataa attttaaata taaaattatt
     5461 aaaagtgttt ataaaaaagt ttttaatgtt gggtggtgga taaatagaca aaataatgaa
     5521 tacagaaaag tatatgatct aaattataaa accaaacaat taggaaatgg ttggatatat
     5581 ttagaagctt attatcgctt tagtgaagat cagcataatt tgatttgttt actaaatagt
     5641 ctcaaggaca actctgaaat tattattact gatgttggta tatatgaaaa aatctaaggg
     5701 cgcattatca attaacctaa ttcttttttt taatatacca ttgtaccatc tcaacaaacc
     5761 ccgactcact tgaatagggt ggattaaatt ttaatttatc atttgtattt ttattggtta
     5821 ccacaaaaga gccatatatt ctatttatgt acttagggaa taaaattttt aatatttttt
     5881 gaatgagaat cggagtctta aataaataaa cgttcttatt tagtcctcta gcaatatatt
     5941 ttattatttc agttgttgat aagggtttac tgtcccctgc aataaaaatt cctgatgctt
     6001 ttttttctat tattattttt aataattcaa ctaagttgtt tacatagacc atagatcgtt
     6061 tattatttat gcaaccaaaa ggaagaaatt tgtatttgtc tacaagttta attaaattta
     6121 acatatttgc cttaactcca ggaccgtaaa caagaggaga tcttacaatt gcaaccttaa
     6181 agttaatatc ttctaagctt ttaacaagtt tttcagcttc atgtttgctt ctaccataag
     6241 aatctttagg atggcaagta gatgattcat cataaattac attattacaa tgcgattctc
     6301 catatacttt aacggtacta agtagaataa aatgttttac tccattattt tttgcttttt
     6361 ttgcaactga gtatgctaaa tctctattaa ttttaaaata ctgttcttca ggaatatcat
     6421 tcatctgatg aactaacgca gctaggtgaa gtactgaatc tactcctaaa aaagatattt
     6481 cttcgatttt attctttaat aaatctagtt caattatttc gagggattta cacgacttaa
     6541 taaaattagt tcctacgaaa ccagatgctc ctgttacaac tactctcatc tcaaatgatt
     6601 taaaattgta ttgttaattt taattctttc attatcaaaa aaccaacccc acttattaaa
     6661 atattttatt gcagaaataa taaatattat aaaaagcctg aaacttttgt tcgcaccact
     6721 ttcatattca tgatataccg aaacaaaagg ataataaagt gttt
//
LOCUS       DAOTJA010000033         6626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_10960_length_6626_cov_5.752549, whole genome shotgun sequence.
ACCESSION   DAOTJA010000033 DAOTJA010000000
VERSION     DAOTJA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_6626_cov_5.752549"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..370
                     /locus_tag="P5241_02850"
     CDS             53..370
                     /locus_tag="P5241_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32460.1"
                     /translation="MFLNAQEAMLAYDNQKIDLHAKIKIKYPKNQITSDAQIIETTVG
                     RLIFNELLPNDFPYQNEVFTNKKLKKLINSIIKQYGFQIAANCLDNIKTIGFKYATVS
                     GLT"
     gene            443..1051
                     /locus_tag="P5241_02855"
     CDS             443..1051
                     /locus_tag="P5241_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32461.1"
                     /translation="MQKQFDEGLLSQEERYTKVIENYTDAMNNVKNVIIQNLAKEVSN
                     PIKIIIDSGARGTPEQLNQMVGIKGIVTTPNGRGIELPVLSSYKEGFNCLEYFIATHG
                     SRKGLVDTAIKTSQSGYLTRKLVDVEQDMIIYNHDCQDTEGLEILRTTHEELEQSFAS
                     RIVSRVLAKNVKLKNGKIIPKNTLIDSEIAKEIVENSDVESI"
     gene            1079..2044
                     /locus_tag="P5241_02860"
     CDS             1079..2044
                     /locus_tag="P5241_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32462.1"
                     /translation="MQGVCQKCYGADLTNGNLIKIGEAVGIIAAQAIGEPGTQLTLRS
                     FHAGGIMTASDITLGLPYIDGLFECRNPKNEALLCEVEGIVDDILTPYNEFNKIVIKI
                     TTKCKNPTYKKYSRSKQNKQFLEPGEKFIYSVNPNKNIKVVPNQQVKIGDPLTIGSIN
                     LKQLFPLVGLVPTENYIKREAQKIYLLAATEIHEKYFELVIRKMFSQVFITNSGDSSF
                     IPGEVVSLANFIETNYNLRNNHKKPATANRLIRGIKKVALNSDSFLSAVSFEETSHAL
                     IKSAIEGKEDKLNGLKANIIIGRLIPAGTGFRKFNKQNNKQNNKK"
     gene            2079..3314
                     /locus_tag="P5241_02865"
                     /pseudo
     CDS             2079..3314
                     /locus_tag="P5241_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
     gene            3325..3714
                     /locus_tag="P5241_02870"
                     /pseudo
     CDS             3325..3714
                     /locus_tag="P5241_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            3890..3975
                     /locus_tag="P5241_02875"
     tRNA            3890..3975
                     /locus_tag="P5241_02875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3923..3925,aa:Ser,seq:tga)
     gene            4097..4423
                     /locus_tag="P5241_02880"
     CDS             4097..4423
                     /locus_tag="P5241_02880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRV32463.1"
                     /translation="MKYCFKLEKNLTLILIIAIIVIIIGGLLHISHTEWLFVITLIIA
                     ILALELFNTSIEHFLDLFHPEINNQVKHIKDLMAGSVAIVSLGALILGIIIFLPKILK
                     LINAIN"
     gene            4428..5207
                     /locus_tag="P5241_02885"
                     /pseudo
     CDS             4428..5207
                     /locus_tag="P5241_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
     gene            5253..5690
                     /locus_tag="P5241_02890"
     CDS             5253..5690
                     /locus_tag="P5241_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV32464.1"
                     /translation="MVEGQMDAIACYQEGVKNVVACSSTALTSEQLNILKRYCSSLIL
                     AYDNDQAGQEATERNIELALNADFEVKVITLNDAKDFSEFMNLHPKQLPNIISLAKPI
                     MEFYFNRAQTLFDINTIKGKKDFMVYFLSKIKYLQNNIIEKSF"
     gene            5691..6065
                     /locus_tag="P5241_02895"
     CDS             5691..6065
                     /locus_tag="P5241_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32465.1"
                     /translation="MDKLSSLLNIKVDLLETELHKLNQTNPYIQKFGITQEDEIINLN
                     TIKTRADILGEKILSSFLHNQDTLKDIVLSVEPYFPSQYSNIIKLIKNNKIQQVLKNQ
                     SVNDIDTNLDINNDDINLINYL"
     gene            6114..6296
                     /locus_tag="P5241_02900"
     CDS             6114..6296
                     /locus_tag="P5241_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32466.1"
                     /translation="MLIKEIQECAKYLKQEHYKNRMLFLQNKIKELESTGSTAMLQEL
                     IKEFKTLTEETQQNEQ"
     gene            6403..>6626
                     /locus_tag="P5241_02905"
     CDS             6403..>6626
                     /locus_tag="P5241_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32467.1"
                     /translation="MNKYKQHKSKIQSTKKSTKKQNKSKTKSVNKHKNHKIKSIRKIK
                     KIVHKPHKQFLPTQQHKKSSKKLSKQEYKE"
BASE COUNT         2682 a          958 c          919 g         2067 t
ORIGIN      
        1 agggtgctat tgactaacat tagattttga taataaatta ggtgaaaata agatattttt
       61 gaatgcccaa gaagcgatgc tcgcttatga taatcaaaaa atagatttac acgcaaaaat
      121 taaaataaaa tatcctaaaa accaaattac gagtgatgct caaattattg aaaccacagt
      181 tggacgatta atattcaatg aactactacc aaatgatttt ccttatcaaa atgaagtttt
      241 tacaaacaaa aaattaaaaa aattaattaa tagtattatt aaacaatatg gatttcagat
      301 agctgccaat tgtttagata atataaaaac aataggattt aaatatgcta ctgtttcggg
      361 attaacttga aacttagctg atctaaaatc tccggctgat aaacaagaaa ttattaaaaa
      421 acatataagt ataaataaag aaattcaaaa acagtttgac gaaggattac taagccaaga
      481 agaaagatac accaaagtaa ttgaaaatta tactgatgca atgaacaatg taaagaatgt
      541 tattattcaa aacttagcca aagaagtttc taatcctata aaaattataa ttgattctgg
      601 tgctagaggt actccagaac agttaaacca aatggttggt ataaaaggta tagttactac
      661 accaaatggc agaggtattg aattaccagt gctttcgtca tataaagaag gattcaattg
      721 tttggaatat tttatagcta ctcatggttc aagaaaaggt ttggtagata cagctatcaa
      781 aacttctcaa tctggatatt taacccgaaa actagtagat gtagaacaag atatgattat
      841 ttataatcac gattgtcaag atacagaagg attagagata ttaagaacaa cccacgagga
      901 attagaacaa agttttgcca gtcgtatagt tagtcgtgtt ttagctaaaa atgtaaaact
      961 aaaaaatggt aaaataatac ccaaaaatac tttaatagat tccgaaatag ccaaagaaat
     1021 agtcgagaat tctgatgtag aatctatatg agcattttca cctttaactt gtaaaaatat
     1081 acaaggtgtt tgtcaaaaat gttatggcgc tgatttgacc aacggaaatt taatcaaaat
     1141 aggtgaagct gtgggcatta ttgctgcgca agctattggc gagcctggta ctcaactcac
     1201 tttacgttca tttcatgcag gaggtattat gacagcatca gatattactc ttggcttacc
     1261 atatatagat ggactatttg aatgtcgtaa tcccaaaaat gaagcacttc tttgtgaagt
     1321 agaaggtatt gttgatgata ttttaacacc ctataatgaa tttaataaaa ttgttattaa
     1381 aataacaaca aaatgcaaaa atcccacata taaaaaatat tctcgatcta aacaaaataa
     1441 acaattttta gaacctggag aaaaatttat ttattctgtc aatcctaata aaaatatcaa
     1501 ggtagtacca aatcaacaag taaaaattgg cgatccttta actataggca gtataaattt
     1561 aaaacagcta tttccattag ttggtcttgt tcctacagaa aattatataa aaagagaagc
     1621 tcaaaaaatt tatttattag ctgctacaga aattcatgaa aaatattttg agcttgttat
     1681 acgcaaaatg ttttcccaag tgtttattac taatagtggc gactcaagct ttatccctgg
     1741 agaagttgta tcacttgcta attttataga gactaattat aatttacgaa acaatcacaa
     1801 aaaaccagct acagccaatc gattaattcg tggtattaaa aaagtagcat taaattcaga
     1861 tagttttttg tctgctgtat catttgaaga aacttctcat gctttaatta aatctgccat
     1921 agaaggtaag gaagataaac ttaatggtct aaaagctaat attattattg gtaggctaat
     1981 tcctgctggt actgggtttc gaaaatttaa taaacaaaat aacaaacaaa ataataaaaa
     2041 ataaaataaa tattgtaata atatttaata ttgtcactat gtctttagtc aaaataactc
     2101 catcaaaaaa accatatgtt tatttagatt atgctgctag tacacccata gacccccgtg
     2161 ttcttaaaag tatgattcct gttatgagat attattatgg taattcttta tctttacatc
     2221 aaaaaggtat ggaagccaaa gaattattag aaagcattcg ggaaggattt gctaaaatat
     2281 taaaagtagc tccacaagaa attatattta catcaagcgc cactgaaagt aataaccttg
     2341 ctataaaagg catagttatg gctgatatga aaaacaatat tgacaaaaat gttgttacct
     2401 cctcacaaga acactcatca ataaatagct tatgtaattg attagaaaaa cacggattaa
     2461 aagtatcaaa agtatctagc accaatgaag gattaattga tataaatcat ttaaaacagc
     2521 tgattaatga taatactatt ttagtttctc tgatttatgt taataacgaa actggtgtaa
     2581 tacaaccgct tgaaaaaata ggcgagttaa ttcaaaaaat tcgccaagat cgattagaaa
     2641 gagaaatcac aactccaata tattttcata ttgatgccac tcaagctttt ggtaaaatag
     2701 ctattgatat aacaaaactc aattgcgatt tactaacagc ttctgctcat aaaatttatg
     2761 ggccaaaagg tgcagcttta ctttatatca aaaaaggtac acatctagaa cctttaatcc
     2821 atggtggggg tcacgagttt ggtatacgtt caagtactgt aaatgtgcca gccatttttg
     2881 gattttttaa agcatttgaa ctatgtcaaa gcctaatgaa acgagattac agaaaatgta
     2941 catctttttc taataaaata ataaaaatat tgcctcaaaa aatatcagga gtccatttta
     3001 atgtaccaaa aaatattcgt cttcctaata ttattaatgt ccgtttcgac tatatagaag
     3061 gggagtcatt ggtatatatg ctcgatactc accatattgg tgtttctaca ggctctgcct
     3121 gcgcttctag ttttttaaaa gccagtgaaa ctctaatttc aatgggtctt aaaccagaac
     3181 aagtaggggg gtctattcgt atttcccttg ggcgttttac taccaaaaaa gaaataaatt
     3241 atttattaga aattttacca caagttgtag aacaattaag aagagtgtct ccctttacac
     3301 ctgcttctac ataaacaatt taatatgaat gaatactatt ccaaacatct cttacaacta
     3361 ttccaaaatc ctaaaaatta tggtaaaata aaaaatcctg atgctatagg tcaagcaggt
     3421 aatttaacat gcggtgatgt tatggctata tatataaaag taggcacaaa tgcaaaaaac
     3481 gaaaaaatta ttaaagatat aaaattcgaa acatttggct gcgtggcagc tattgccaca
     3541 tcgtctatga ttactacatt agccaaaggc aaaacattag acaaagcttt acaaattacc
     3601 aacaatgata ttattaataa attagatgga ctgccaactg ccaaagtaca ctgttcatta
     3661 ttagcaacag atgctttatt tgaagcaata tatgattatt ataaaaaaca tgagcctaac
     3721 aaaataacag aagaattgga acaaaaacac caaaacatat taaaaaatca aacaattatt
     3781 gaacaacgtt ataacgaatg aataaaacaa gaaaaaaagg aattaaacaa aaactaaata
     3841 tttttcatta ttatttagta tcaaaatatt tacaacattg ggttttaacg gagggttggc
     3901 agaatggcta atgcgtcggt cttgaaaacc gatgggcgaa agcccttgca ggttcgagtc
     3961 ctgtgccctc cgccataact attttctctt tttacaataa ataaatctaa atactaattt
     4021 tttaatacca agcaatataa atatgaatga tttttttaat caattttttg cgagttgaaa
     4081 atacgcctga aatggaataa aatattgttt taaattagaa aaaaatttaa ctttaatact
     4141 tattattgct attattgtaa taattatagg agggttgcta catatttctc atactgaatg
     4201 gctttttgtt ataactctca taatagcaat attggcattg gaattgttta atacaagcat
     4261 tgaacacttt ttagatttat ttcatccaga aattaataat caagtaaagc atattaaaga
     4321 tttaatggct gggagtgtag ccattgtttc cctaggcgct ttaattcttg gtattattat
     4381 ttttttaccc aaaattttaa aactaataaa cgctataaac taaatttatg gccgatgtca
     4441 gagatgaaat taaacaaaaa ctaaatattg ttgatgtagt gggtagctac ataaaacttc
     4501 aaaaagccgg ggtaaattac aaagcgcttt gtcctttcca caaagaaaaa accccgtctt
     4561 ttattgtgtc gccccaaaga caatttgctc attgttttgg atgtaatttt tcaggtgata
     4621 tttttacctt tgttatgaaa atagaaaatg ttgatttttt tactgcttta aaaattttag
     4681 ccaataaagc cggagttaat ttatcgcaat atcaaagtaa agaaaattcc atcaaaaaaa
     4741 ctctatttga aattcaagaa gctgcattaa tattttttga acaaaaatta aaagaaaata
     4801 caagtgctta taattattta aaaaatagag gattaagtaa tcaaattatt caagagtttc
     4861 atattgggtt tgctcctgat gaatgaagat cattaactga atatttaatt agcaaaggct
     4921 tcaaactaag tgatattgaa aaaactggat taattattcg taaaaatgaa gcagccaata
     4981 taacagatat taattcgctt acaactaaat tttttttcga ccgttttaga tctcgaatta
     5041 tgtttcccat ttttaatttg tcaaatgaac cagctggttt tacgggaaga attttccaag
     5101 ccgacaaaaa tattccctta aaaactatca aaaacataga agaaaccggg aaatatataa
     5161 attctccaga aactattatt tttaacaaaa gcacactttt gtttttatta aataaaacca
     5221 aaaattttat ccaagaaaaa aatgaagcta ttttggtaga aggacaaatg gatgcaattg
     5281 cttgttatca agaaggagtc aaaaatgttg tagcatgttc tagcacagct ttaacatcag
     5341 aacaattaaa tatattaaaa cgctattgta gctctcttat tttggcatat gataacgatc
     5401 aagctggaca agaagccaca gaaagaaata ttgaattagc tttaaatgct gattttgaag
     5461 taaaagttat tactctcaat gatgccaaag atttctctga atttatgaat cttcacccaa
     5521 aacaattgcc caatataata tctttggcaa aaccaattat ggagttttac tttaatagag
     5581 cacaaacact ttttgatatt aatactataa aaggcaaaaa agattttatg gtgtactttt
     5641 tatcaaaaat taaatactta caaaacaata taatcgaaaa atcgttttga atggacaagc
     5701 tatctagtct attaaacata aaagtagatc ttttagaaac agagctacat aaactaaatc
     5761 aaacaaatcc atatattcaa aaatttggga ttactcaaga agacgaaata ataaatctga
     5821 acaccataaa aacacgagca gatattttgg gagaaaaaat tttatcaagt tttttacata
     5881 atcaagatac attaaaagat atagtattaa gtgtagaacc ttattttcct tcgcaatatt
     5941 caaatattat caaacttatt aaaaataata aaatccaaca agtgttaaaa aaccaatccg
     6001 taaatgatat tgatactaat ttagatatta acaacgatga tataaatctt attaattatc
     6061 tttgaattct tggctccaaa gatatacaaa atcaaactgc atctaataca aatatgctaa
     6121 taaaagaaat tcaagaatgc gccaaatatc taaaacaaga acattataaa aatcgtatgt
     6181 tgtttttaca aaacaaaata aaagaattag aatcaacagg ttctacagct atgctccaag
     6241 aacttataaa agaatttaaa acattaacag aggaaactca acaaaacgag caataattgc
     6301 caataaataa tatactattc tttttagttt ctcatttttc atctttaatt tttactttta
     6361 ttattttaaa aactatttaa aaattatttt aaaaatttta atatgaacaa atataaacaa
     6421 cataaatcaa aaatacaatc tacaaaaaaa tctacaaaaa aacaaaacaa atcaaagaca
     6481 aaatctgtaa acaaacacaa gaaccataaa ataaaatcca ttagaaaaat caaaaaaata
     6541 gttcacaaac cccataaaca atttttacca acacaacaac ataaaaaaag tagtaaaaaa
     6601 ctaagtaaac aagaatacaa agaaat
//
LOCUS       DAOTJA010000034         6397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_11357_length_6397_cov_6.153737, whole genome shotgun sequence.
ACCESSION   DAOTJA010000034 DAOTJA010000000
VERSION     DAOTJA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11357_length_6397_cov_6.153737"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="P5241_02910"
     CDS             <1..589
                     /locus_tag="P5241_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HRV32468.1"
                     /translation="QVIALLIFISYFFIPQLKFLFEFLANAKAKYGVILIMFTTGFSG
                     GVVPDLLNYIFKKNYLIDYKKVFMQFLFWFYKGFEINLFYTLLAIYIGNSNNFSIVFT
                     KVLIDEFLYTPFWAVPTMLFCYGWIEKGKIQLSIFSEIILKVKQDYFVVLLLNWIIWI
                     PSVAVVYSLPLLLQMPIQNVILMIWGILIVFFAKK"
     gene            657..1013
                     /locus_tag="P5241_02915"
     CDS             657..1013
                     /locus_tag="P5241_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32469.1"
                     /translation="MEGILALFIPILTILSLFAYVIIRFYLSSKERKYLIERGLDAET
                     IKALFTKPTNPDNKYKYLRYGLFAIGIGAGIALGDILNDVFNRPSLQFFTILVFIGIV
                     FLVSYHLESKEKSSEK"
     gene            complement(1426..3978)
                     /locus_tag="P5241_02920"
     CDS             complement(1426..3978)
                     /locus_tag="P5241_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32470.1"
                     /translation="MAKSNTQKSKSLGQQKSGLETFIDNIKKYDNYFALGVILILILF
                     HFYPLLFDGKTFVSGDIQATSGPKSDLPPEYRLWNPYIWSGMPGSNVVFWYNLISMIT
                     SYARTIWGAFFSVPYANWLFFVILLGFNTYLLMKFITKKRTVALFSSVSTIFSTGLIL
                     YIMIGHVSKTAVIAWFPLVLYLIFKMQEKIKLIHFAVLSFALTMLFNPWHVQMIYYIY
                     MFLVLYYLYYIVSYLINKEQDKLKSLIKSGLVLTAATIVGLGINIYTYLNLYEYSKYS
                     TRGTKSILEQKIDKIKDPQLKEEIKHAEDDQYRYMTNWSFSVEELTTFFIPSFYGFGN
                     STYEGELTNYQPYNVNTYFGQMPFVDVPQYMGIIVTVLAILAFVIDWKNKFTRFLLIV
                     SVLTLFISFGRNLPIIYNLFYYFAPGFNKFRVPSLILYLLQTVFPILAGLALSKIIDC
                     IKENKPIFKLDILQKVLYVFAALVVLVLILSGSLKDLYISWISGIEKAAQLKPLYTYM
                     ADMFVADLIISLVLITLLIVSLFLLISKKINYFVFVSLLLLFTFIDLHRIAKRAEHYT
                     DYEEFQYQFTEPAYIKYLRGQPKQEPYRIINIKQDGSLGSLNNNANFHEKYRIEDIYG
                     YSAIKPRLIQDYMDVISPINETFWKLANVKYIISDKAYRFDNINLEIASVVDGNVILV
                     NKDFIPRAYFVDTVKVADELEYLYLVKDNAFNPKTTAFIHTKDFDLSKVKVTNNKSYT
                     KTLAYTPDKREYELYADDDNFLVISDTYYPTGWKYSLDGREIEPIRTNHAYRGFLIPK
                     GRHKLIMSFEPTSFYIAKYVALILSLIILGIIILYFVKYLQQKQIEQEKSIS"
     gene            complement(3988..4794)
                     /locus_tag="P5241_02925"
     CDS             complement(3988..4794)
                     /locus_tag="P5241_02925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV32471.1"
                     /translation="MKYDPVKNIFEKFINKSPIFRKSFYKILDTLFLRAWYIHRELKE
                     IRRVFKNQNINIYDAGCGYGQYSYYLLKKLTPCNIVSVDIKEDWLNNLKPFFQKYNNI
                     KLKVEDITQINDESIYDLVLCVDVIEHIVDDQKVFNNFAKMLKKDGFVVISTPSIYGG
                     SDAHDEHDDSFIEEHARNGYSYEDIENKAANAGLKIHKFKFSYGAFGSLSWKLAIKYP
                     ILMVNFSKIMLLFLPFYYAVFILPILLLMFIDTQVNNKLGTGIIVTLKKI"
     gene            complement(4791..6068)
                     /locus_tag="P5241_02930"
     CDS             complement(4791..6068)
                     /locus_tag="P5241_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32472.1"
                     /translation="MNKVVVFGPGPMFKGGIANYTISLAKALDKLENTKVYIVSWTNQ
                     YPSIIPRDFVDKSSKTDLLEGTDINVIYVTDYNKPNSWNKTVEIIKNIDPQKVIFQWA
                     IAIQGLPMGYIAKKLKKTTKAEIIFDLHVVKQKESSIIDKAMLKEALKIPDTFIVHAL
                     KTYDELKELFPNKNYYLSYDGIRSNEPDTKNVLKLYHPIYDMFTPDPNLDVEKLKQEI
                     GLNKYVFLFFGFIRKYKGLHNVILAFNELLKRRKDVSLLIVGESFWHTLDKRKLSTKI
                     KKIIFNVLKKILLRKSDDESNYNPLALVDELNLHDYVKIFNQYIPNEDVHKYFQISNV
                     NVLFYLTATPSGVESISYNFHMPIVATKVGHFPETIQEGYNGYLAEPNDIISMADAME
                     KAITNPIDRNNVKATAEKMSWENYAKTIMKDII"
BASE COUNT         2258 a          908 c          780 g         2451 t
ORIGIN      
        1 tcaagttata gcgttactta tttttatatc atactttttt atcccccaat taaaattttt
       61 atttgaattt ttagctaatg ctaaagcgaa atatggtgta attctgataa tgtttaccac
      121 aggtttttct ggtggtgttg ttcccgattt attaaattat attttcaaaa aaaattactt
      181 aatcgattat aaaaaagttt ttatgcaatt tttattttgg ttttataaag ggtttgaaat
      241 aaaccttttt tacacattgt tagctatcta tataggaaat agtaataact tttcaatagt
      301 atttacaaaa gtattaatag atgaattttt atatactcct ttttgggctg ttccaacaat
      361 gttgttttgt tatggctgga tagaaaaagg caaaattcaa ctttcaatat tttctgagat
      421 tatattaaaa gttaagcaag attattttgt tgtattacta ctaaactgga taatctggat
      481 accatctgta gctgttgtat actcacttcc actcttactc caaatgccta tccaaaatgt
      541 aattttaatg atatggggaa ttttaatagt tttttttgct aaaaaataaa ttttttgtaa
      601 cctataaaaa aatatatagt caaaactatt aaataaatat taaattagag gttacaatgg
      661 aaggcatatt agcattattt ataccaattt taacaatatt atcactcttt gcttatgtca
      721 ttataagatt ttatctttca tctaaagaaa gaaaatattt aatagaaaga ggattagatg
      781 cagaaacaat caaagctttg tttacaaaac caactaatcc cgataacaaa tataaatatt
      841 taagatatgg attattcgca attggaattg gtgcaggtat agctttgggt gatattttaa
      901 atgatgtttt taatcgtcca tccttacaat tttttacaat tcttgttttt ataggtattg
      961 tttttttggt tagttatcac ttggaatcaa aagaaaaaag ttccgaaaaa tagttcaaca
     1021 aactttttta ataaactttt tgcttgaaat taaggatata atcattagtt tttgataatt
     1081 tgaaagttat atatataaga tagtaatttc aaagaaacat tattccttat ttagatataa
     1141 ataaatactt ttatgattta ttagatttga aaatttaact ttacttatat tgtttcttta
     1201 aaatccacac aatactattc atttttaaat attattgtat aatttatttg aatttacagc
     1261 aattactttt ttagtaaaga gcttattaat tttgagaata ataaagcttt aaattttcta
     1321 aggaataaaa gaaaaacggc catctaatgt gttttgagta taacatattt agacgaccgt
     1381 ttaacttttc ctatttgcat ctataaatct atagactttt cataatcaac ttatcgattt
     1441 ttcttgctct atttgctttt gttgaagata tttcacaaaa tataatataa ttattcctaa
     1501 aataattaaa cttaatatta aagctacata ttttgctata taaaacgatg ttggttcaaa
     1561 actcattatt aatttatgcc tgcccttagg aatcaaaaat cctcgatatg cgtgatttgt
     1621 tcttatcggt tcaatttctc taccatctaa ggaatatttc caaccagttg gataatatgt
     1681 atcacttatt acgaggaaat tatcatcatc tgcatataat tcatattctc ttttatcagg
     1741 agtataagct aatgtttttg tataactttt attatttgtt actttcactt ttgataaatc
     1801 aaaatctttt gtatgtataa atgcagttgt ttttggatta aaagcattat cctttactaa
     1861 atataaatat tccaattcat ctgcaacttt tacagtgtca acaaaataag ctctcggaat
     1921 aaaatcttta tttaccagta ttacattgcc atcgacaact gaagctattt ctaaattaat
     1981 attatcaaac ctataagcct tatcggaaat aatatatttt acattggcta acttccagaa
     2041 agtttcgtta attggactta taacatccat ataatcttgt attaaccttg gtttaattgc
     2101 tgagtaaccg taaatatctt caattctgta tttttcgtgg aaatttgcgt tattgtttaa
     2161 tgaacctaag gaaccatctt gcttaatatt aataattctg tatggctctt gttttggttg
     2221 gccacgtaag tatttaatat atgctggctc tgtaaactga tattgaaatt cttcgtaatc
     2281 tgtataatgt tctgcccttt ttgctattcg atgtaaatct ataaaggtaa ataacaataa
     2341 tagagataca aaaacgaaat agttaatctt tttgctaatc aataagaata aacttactat
     2401 tagtaaagtt ataagtacta aagaaattat taaatcagct acaaacatat ctgccatata
     2461 agtgtaaaga ggttttaatt gtgctgcttt ttcaattcca cttatccaac taatatataa
     2521 atcttttaag ctacctgaaa gtattaatac taaaactact aaagcagcaa atacatataa
     2581 aactttttga agaatatcta atttgaaaat aggtttattt tctttgatac aatctataat
     2641 tttgcttaat gctaatcccg caagtattgg gaaaactgtt tgtaatagat agagtattaa
     2701 tgatggaact ctaaacttat taaatcctgg tgcaaaataa tagaataaat tataaatgat
     2761 tggtaaattt cgaccaaatg atatgaataa agttaaaaca gatactatta ataaaaatct
     2821 tgtaaattta tttttccagt caatcacaaa tgccaaaatt gccaataccg taacaattat
     2881 tcccatatat tgaggaacat caacaaaagg catttgtcca aaatatgtat taacattgta
     2941 tggttgatag tttgttaatt cgccttcgta tgtgctatta ccaaacccat aaaaagaagg
     3001 tataaaaaat gttgttaatt cttcaacaga aaatgaccag ttggtcatat atctatattg
     3061 atcatcttcg gcgtgtttta tttcttcttt cagttgtgga tctttaattt tatcaatctt
     3121 ttgctctaaa atactttttg ttccacgtgt tgaatatttt gaatattcgt agagattcaa
     3181 ataagtatag atattaatcc ctaaacctac gattgtagca gcagttaata caagtccaga
     3241 ttttataaga gattttaatt tatcctgttc tttgttaatt aaatatgata cgatataata
     3301 caaataatat aagactaaaa acatgtaaat gtaataaatc atctgcacat gccatggatt
     3361 aaaaagcata gttaatgcaa aacttaaaac agcaaaatgt ataagtttga ttttttcctg
     3421 cattttgaat attaaataaa gaactaaagg aaaccacgca ataacagccg ttttagaaac
     3481 atgcccaatc ataatatata gaataagtcc agtactaaat attgttgaaa ctgatgaaaa
     3541 taaagctaca gttcttttct ttgttataaa tttcatcaaa agatatgtat taaaaccaag
     3601 taaaattaca aaaaataacc agtttgcata tggaactgaa aaaaatgctc cccaaatagt
     3661 tcttgcataa cttgttatca tagatattaa attataccaa aaaacaacat tactacctgg
     3721 cataccggac cagatgtatg ggttccacaa tcggtattca ggtggtaaat ctgatttagg
     3781 accacttgtc gcttgtatgt cgccagatac aaatgtttta ccatcaaata ataatggata
     3841 aaaatgaaaa agaattaaaa ttaatataac tcctaacgca aaataattat catatttttt
     3901 gatattgtca ataaatgttt ctaaaccaga tttctgttgc cccaacgatt tggacttttg
     3961 tgtattagat tttgccatct tataaaccta tatttttttt aatgttacta ttattcctgt
     4021 acctaatttg ttatttactt gtgtatcgat aaacattaat agtaaaattg gaagaataaa
     4081 aactgcataa taaaaaggta aaaataacaa cattattttt gaaaaattta ccattaaaat
     4141 aggatatttt attgctaatt tccacgataa cgaaccaaat gcaccatagg aaaatttaaa
     4201 tttatgaatt tttaatcctg cattggctgc tttattttca atatcttcgt aggaataacc
     4261 atttctcgca tgttcttcta taaaactatc atcgtgttca tcatgtgcat cactaccgcc
     4321 atagattgat ggagtactaa tcactacaaa gccatctttt ttaagcatct ttgcaaaatt
     4381 attaaaaact ttttgatcat ctacaatatg ttcaataaca tcaacacata aaactaaatc
     4441 ataaatactt tcatcgttaa tttgtgtaat atcttcaact tttaatttta tattgttata
     4501 tttttggaaa aaaggcttta agttatttag ccaatcttcc ttaatatcaa cagacacaat
     4561 attgcaaggt gttaattttt ttaacaaata ataactgtat tgcccataac cacaaccagc
     4621 atcatatata tttatatttt gatttttaaa tactctacga atttctttta actccctatg
     4681 tatataccag gctcttaaaa aaagtgtatc caatatttta taaaatgact ttctaaaaat
     4741 tggcgattta ttgataaatt tttcaaaaat attttttacg ggatcatatt tcatatgata
     4801 tccttcatta tagttttagc gtaattttcc cagctcattt tttcagcagt tgctttaaca
     4861 ttatttcgat ctataggatt agtaattgct ttttccatag catcagccat tgatataata
     4921 tcatttggtt ctgcaagata tccattatag ccttcctgaa ttgtttccgg aaaatgccca
     4981 acttttgtag caacaattgg catatggaaa ttgtaagaaa tagattctac tccagaaggc
     5041 gttgcggtta gataaaacaa aacatttaca ttggatatct ggaaatattt atgaacatct
     5101 tcatttggaa tgtattgatt gaatattttt acataatcgt gcaaattcaa ttcatctacc
     5161 aacgcaaggg ggttgtaatt agattcatca tcactttttc ttagaagaat ttttttcaat
     5221 acattaaaaa taattttttt tatttttgtt gaaagttttc ttttatccaa agtatgccag
     5281 aaactttcac ctacgataag taaagaaaca tcctttcttc tttttagcaa ctcgttaaat
     5341 gctaaaatta cattatgcaa gcctttgtat ttacgaatga atccaaaaaa taaaaataca
     5401 tatttattta aaccaatttc ttgtttcaat ttttcaacat cgagattagg gtcaggtgta
     5461 aacatatcat aaattggatg atataatttt aacacatttt tggtatctgg ttcatttgat
     5521 cttataccat cgtaagaaag ataataattt ttatttggga ataattcttt taattcatca
     5581 taagttttta aagcatggac aataaatgta tcaggaattt taagagcttc tttaagcata
     5641 gctttatcaa taatactact ttccttttgc ttgacaacat gcaaatcgaa aataatttct
     5701 gctttggttg tttttttcaa ttttttagct atgtatccca taggcaatcc ctggatagca
     5761 atagcccatt gaaaaattac tttttgagga tcgatattct taataatttc aactgtttta
     5821 ttccagctgt ttggcttatt gtaatcagta acataaatta cattaatatc cgttccttca
     5881 agtaaatcag ttttactact tttatcgaca aaatcacgag gaataataga agggtattga
     5941 tttgtccatg atacgatata aacttttgta ttttctagtt tatcaagtgc tttggctagt
     6001 gaaattgtat aattagcaat accaccttta aacattggtc caggaccaaa aacaactact
     6061 ttattcattg tattacctat ataaataaaa tcaatttgtt aatatacaaa aaaaaatata
     6121 attctttaaa taaaaatatt taatacctac aaaattgaaa aaacggttgc cgcgtattgc
     6181 aacaaccgtt atatattttt aattcatttt acactttttg ctttacaaca tctaatattt
     6241 tacgcctcaa catttaatta aattttatat ttatttatca tctcttgtaa attaactgtt
     6301 aaattactta aatctattgc tgcctgtgaa atctggttga ttccttgtga tgtctcctgt
     6361 attactgttg ttattccttc tatacttcta cttatct
//
LOCUS       DAOTJA010000035         6028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_12069_length_6028_cov_6.305207, whole genome shotgun sequence.
ACCESSION   DAOTJA010000035 DAOTJA010000000
VERSION     DAOTJA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12069_length_6028_cov_6.305207"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            686..1252
                     /locus_tag="P5241_02935"
                     /pseudo
     CDS             686..1252
                     /locus_tag="P5241_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
     gene            1433..1639
                     /locus_tag="P5241_02940"
     CDS             1433..1639
                     /locus_tag="P5241_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRV32473.1"
                     /translation="MQFIKTKENIAVVIDYAHTPDSLEALYTNLRNLGYNNIIGVLGA
                     TGAMQSQINTLGIVDTAKGGTRDE"
     gene            1652..1981
                     /locus_tag="P5241_02945"
     CDS             1652..1981
                     /locus_tag="P5241_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32474.1"
                     /translation="MGQIVEKYCYKIILTNEDPYDEDPVKIIEEIKAGIKDQNKVEVV
                     VDRQLAIRKAIDLAKSGDIIVITGKGCEKTMMVKGGARGSKSIPWTGDYELSKIYLKD
                     LGKEILF"
     gene            2088..3224
                     /locus_tag="P5241_02950"
                     /pseudo
     CDS             2088..3224
                     /locus_tag="P5241_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            3426..4199
                     /locus_tag="P5241_02955"
     CDS             3426..4199
                     /locus_tag="P5241_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV32475.1"
                     /translation="MWICEKCKHQEVIGSIDELDEKAYIKTTFYYLRHGESLSNIKNI
                     ASCYPTPFVDHITDKGKDQVRKTIENFQKSNIKIDLIVTSDLLRTKETAEMFSDVIGC
                     PIVYEERLREINFGEYNGKSMKKVLEFIKNESSKNFDAFFPGGESLAKKQSDAISLIL
                     DLQKQYARQNILIVSHKGVIQLIEAKLTGRDRRDAVGMLLDQSWPKNAELKKMSFKNA
                     PYNLETQDMDLHKPYVDEFYLKCPKCNSIMKRTKEVIDC"
     gene            4347..4508
                     /locus_tag="P5241_02960"
     CDS             4347..4508
                     /locus_tag="P5241_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV32476.1"
                     /translation="MAVGTCLDMPAPFKNVMALGLILDENGEKMSKSRGNVVDPEVII
                     DKYGADALR"
     gene            4824..4964
                     /locus_tag="P5241_02965"
     CDS             4824..4964
                     /locus_tag="P5241_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32477.1"
                     /translation="MRRSRRRLQQPNDAKDYFVASLVLGVVLKEMSKLIAPFVPYMGE
                     IL"
     gene            4989..5591
                     /locus_tag="P5241_02970"
     CDS             4989..5591
                     /locus_tag="P5241_02970"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRV32478.1"
                     /translation="MNKTSFTEKSVHLATIENADMSLIDTKLMVSMNTLRDIANKALK
                     QRQELGIKIRQPLSSISLKKRINLSKNMKTILLDEINIKKILFNPKQKDEIVLDKKLT
                     NELIEEGQYRELVRTIQGIRQELNLVPKDKIILSFMNCDFKNQKFVNKYQTKLLSEVK
                     ANKLIFVPIDKFIRQQEIELQNQKILLTIALVKKAKKHNN"
     gene            5676..6025
                     /gene="ssrA"
                     /locus_tag="P5241_02975"
     tmRNA           5676..6025
                     /gene="ssrA"
                     /locus_tag="P5241_02975"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2207 a          670 c         1061 g         2090 t
ORIGIN      
        1 tgataaaatc tattgcagtt tattgtctta ttttgctttt tcttattttt tattttgttt
       61 cttttgtttt attggaatag tttttgtttt ttttattttg tttcttttgt tttatttgtt
      121 tatttattgg tttctatttt ccagtatcta tttacagggg tcaaacccct gtaagttttc
      181 cacaggagta accaaaaccc ctgataaaat ctattgcagt ttattgtctt attttgcttt
      241 ttcttatttt ttatttgttt ttcttgtttt atttcttatt ttgtttcttt tattttattt
      301 gtttatttta tcatatatca tattttaata caataagata ctataatttt tataaatagt
      361 gatttacatc attttatata tttggatatt ttgtatctga atatatcagt attagtgtat
      421 gttaagtgtt aaaataatcc aaaataggta atgtgtattt tgaagtaaat tagaatattg
      481 tgattttgta ttggtataca aaggttgatt ttattgtagt tttttgctat aattatagta
      541 tctaaatcct tttaataaat ggcaatttaa ttttatgaaa tttatacata aattgttaaa
      601 taaagtgttt aaaattgttc gtgatataaa agatatattt tttgtgtatc ttggcaagtt
      661 tatattggga tttagacaaa aaaatatggt gattattggt gttactggta caaaaggtaa
      721 aactactgtt gtaagtttga tgtctcatat tttacacaat gccggtgttc cttttggtag
      781 tttttctact attgaacaaa atattaatgg caagataatt cgtaatgatt ccaagcttac
      841 aatgcctggc agagcagcta ttaatttgtt tttaaaaaag gcgtataata aaggagcgag
      901 agttggttta atagaagtaa catctgtagg tttgaaacaa catagaggtg attttattga
      961 atgagatgct gctgtactaa cgaatttaca gccagaacat ttagaaattc atggaggttt
     1021 tgaaaattat aagatgtgta agggtttatt gtttaaaatg ttaaaaaagt accctgtcaa
     1081 atatatacaa aataaaaaat atttaaaaac tattattgta aattttgatg atccgaatgc
     1141 tgattatttt ttacaattca aggcggataa aaaagttgtt atttctttaa accctgaacc
     1201 agatatcgct aaaattaaat tgatggattt ggaattatta aaaccaacat gatatcgagc
     1261 agataattta ggtatagata tagaaataga caaaagagta taccatagta atttattagg
     1321 tgattataat gtttataatg ttttattttg tatagcttta tctcgctatc taaatataaa
     1381 tgacgatatt gtttttgaag cgttacaaac ttatactggt actcctggta gaatgcaatt
     1441 tataaaaact aaagagaata ttgctgtggt gattgattac gctcataccc ctgattcctt
     1501 ggaagctctt tatactaact tacgcaattt gggatataat aatataattg gagttttggg
     1561 ggctacagga gcaatgcaaa gtcaaataaa tactttgggt atagttgata cagcaaaagg
     1621 tggcacaaga gatgaatgaa aaaggcctgt aatgggacaa atagttgaga aatattgtta
     1681 taaaattatt ttaacaaatg aagatcctta tgatgaagac cctgtaaaaa ttattgaaga
     1741 aattaaagca ggtataaaag accaaaataa agtagaagtt gtggttgata gacaattggc
     1801 tatacgtaaa gccattgatt tagcaaaaag tggcgatata attgttatta ctggtaaagg
     1861 gtgtgaaaaa actatgatgg tcaagggtgg agctcgggga agtaaatcta taccctggac
     1921 aggagattat gaattatcta aaatttattt aaaggattta ggaaaagaaa ttttatttta
     1981 aacttatata aatggtagaa caaaataata atgaacaaga acaaaattct aaattagaat
     2041 ttgtggcaaa agagcctcaa attttagaat tttgagacaa aaatacaatt tttgacaaaa
     2101 ctttggcatt aagaaaaact tctaaagaat ttgtgttttt tgaaggaccg cctacagcta
     2161 ataacaagcc aggtgtacac catgtattag ctagagttta taaagattta atttgccgct
     2221 ataagacaat gagagggttt ttagtaaacc gtaaatctgg ttgggatact catggattgc
     2281 cagtggagat agccattgaa aaagccttag gatttaattc caaaactgat attgaaaatt
     2341 atggagtaga taagtttaat aaattagcca aagaatcagt acaaaaatat gttaaagatt
     2401 gggaggaaaa tactcgcaaa attgcttatt gattagactt aaaacatcct tatgttactt
     2461 ttgatactaa atacatagaa acattgtgat ggattttaaa acaaattgat agtaaaaatt
     2521 tactatataa aggttataaa atagtgccat attgtccacg atgcggtact agtttgtcga
     2581 gtcacgaagt ggcacaaggt tatagaactg ttaaagatcc atctgtttat gtaaaaatga
     2641 aattaaaaaa tagctctgat attgtgatgg gtaatgttag tttattggtt tggactacta
     2701 ccccttgaac attacctgcc aatgtagcag tggctattaa taaagatttt gattatggag
     2761 tatatcaact tgataatgaa tattttatta gttatacaga tttaactaaa ctatcatttt
     2821 tggctaacaa agatattaag aaaattaaag agataaaagg aagcgagctt ttgggtagaa
     2881 aatatgaggc attgtttgaa cacaaaaaga tattatcaca aaatgaacaa atatattcgg
     2941 tagtgccagc tgattttgta agtggtgaag atggcactgg attagtacat attgctccag
     3001 cttttggtat tgatgataaa gcgcttggtg ataaattagg actacctttg atattaaacg
     3061 tagatacttc tgggcatata ataaatgatg aaaatatttt aactgatata gttggaatgt
     3121 ttttcaaaga ggcagatccg attattatga aagacttagt taaaagaaat ttggttttgc
     3181 attataattt acaaggtatt gaacatgaat atccattttg ttgaagatgt gatactcctt
     3241 tactttatcg agcttctgat acttgattta ttcgaatgag ttctcttaga gaagccttga
     3301 tacaaaataa tgaatcagta aattgatatc cgagttattt aaaagaagga agatttggcg
     3361 aatgaattaa agatgccaaa gattgagctt tgtcgcgaga aagatattga gggacacctt
     3421 tgccagtgtg gatctgcgaa aaatgtaaac atcaagaagt cattggctct attgatgagc
     3481 ttgatgaaaa ggcatatatc aaaactactt tttattattt aagacacgga gagagcttat
     3541 ctaatataaa aaatatagcc tcttgctatc cgacaccttt tgtagaccat attactgata
     3601 aaggaaaaga tcaagttcgt aaaacaattg aaaatttcca aaaatcaaat ataaaaattg
     3661 atttgattgt aacgtctgat ttattaagga caaaagaaac agctgaaatg tttagtgatg
     3721 tgataggctg tcctattgta tacgaagaac gactgagaga aattaatttt ggagaatata
     3781 atggtaagtc aatgaaaaag gttttggagt ttattaaaaa tgaatcaagt aaaaattttg
     3841 atgctttttt cccaggcggg gaaagtttgg caaaaaaaca aagtgatgct atatcattga
     3901 ttttggattt acaaaaacaa tatgccagac aaaatatttt gatagtttca cataaaggtg
     3961 taattcaact aatagaagct aaattaacag gtagagaccg tagagatgca gttggaatgt
     4021 tattagatca aagttggcct aaaaatgctg aattaaagaa aatgagtttt aaaaatgcgc
     4081 cgtataatct agaaacacaa gatatggatt tacataagcc gtatgttgat gagttttatt
     4141 tgaaatgtcc aaaatgtaat agtataatga aaagaacaaa agaagtgatt gattgttgat
     4201 ttgattctgg ggctatgcct tttgcgcaag tgcattatcc ttttgaaact aatcaaaact
     4261 ctggtttggc tcaaaataaa tttaaatacc cagctgattt tgtatgtgaa ggtattgatc
     4321 atacgcgtgg ttgattttat acattattgg ctgtaggaac gtgtttagat atgccggctc
     4381 catttaaaaa tgttatggct cttggtttaa ttttagatga aaacggagaa aaaatgtcca
     4441 aatccagagg taatgttgta gacccagaag taataataga taaatatggt gcagatgctt
     4501 tgcgttgata tttatatgct gttaattcgc cttcggaaac taaaaaattt tctgaaaaag
     4561 atttagtgtc tttacaacgt cgttttctta atactttatg aaatgtttat aaattttatg
     4621 aaacttatgc ttctacaaaa aagtgtgatt tgacgttaaa agaaataaag aaaaagaaaa
     4681 aagatttaac agatttagat agatgaatta tatcaaaatt agaatcaaca aaattggaat
     4741 atattaaata tttagaccaa tatgatactt ttaaagtaac tcaattattg gattcgtttg
     4801 ttgatgattt atcaagatga tatttgcgta gatcgagaag acgattacaa caaccaaatg
     4861 atgcaaaaga ttattttgtg gcttctttag ttttaggtgt tgtgttaaaa gaaatgtcta
     4921 aattgattgc cccttttgta ccatatatgg gcgagatact ttgacaatct ttaaaaaagg
     4981 caccccatgt gaataaaaca tcttttacag aaaaatctgt acatcttgcg acaatagaaa
     5041 atgctgatat gtctttgatt gataccaaat tgatggtttc tatgaataca ttgcgtgata
     5101 tagcaaacaa agcattaaaa caaagacaag aattgggtat taaaattaga cagcctttgt
     5161 ctagtattag tttgaagaaa agaattaact tatctaaaaa tatgaaaact attttgttgg
     5221 atgaaataaa tataaagaaa atattattta atcccaaaca aaaagacgag attgtcttag
     5281 ataaaaaatt aactaatgag cttattgagg agggacaata tagagaattg gtgcgaacta
     5341 ttcagggtat tagacaagaa ttaaatttgg tgccaaaaga taaaattatt ttatctttta
     5401 tgaattgtga ttttaaaaat caaaaatttg ttaataaata tcaaacaaaa ttgttgtcag
     5461 aagttaaggc aaataaattg atttttgtgc ctatcgataa atttattaga cagcaagaga
     5521 tagaattgca aaatcaaaaa attttattaa ctattgcctt agtaaaaaaa gcaaaaaaac
     5581 ataataatta aaaattatta tgttatgtat atctatagga ttcatctatg attacttgca
     5641 aaatatagaa aattatgata atatttagat gtattgggga tgtgtggttt agataaaaaa
     5701 tggttctttg ataatgcaaa tcaaggaggc tttgtcacct tgtaaaacat caaagcaaca
     5761 aataactgtc aaatcttatc aaccttctta cgctttggcg taaagggtta tagacaacct
     5821 ctaaggaaga ctcctgatag gccttagagt taattttcag gatgcgctaa acactgttgg
     5881 ttgtgtttag ttaagataaa taaccaacta ttgaaaaata tttttgtctg tttttaatat
     5941 ttttcaaaaa cttaaacaga ctaaatttgt agagttatta aagaaagttt tttagacgcg
     6001 ggttcaattc ccgccatctc cacagaaa
//
LOCUS       DAOTJA010000036         5711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_12784_length_5711_cov_5.203147, whole genome shotgun sequence.
ACCESSION   DAOTJA010000036 DAOTJA010000000
VERSION     DAOTJA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12784_length_5711_cov_5.203147"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="P5241_02980"
     CDS             complement(<1..677)
                     /locus_tag="P5241_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32479.1"
                     /translation="MSYADTFDKQFDEQEINNLENKILRQRKILQELGFVDDSIIEEY
                     KSVDERLTFLQKEKSDLKQAVDDLQHIIKELTIKIHKQFNDSIKEINKEFNKYLQILF
                     GGGKGSVEIVQSSNNLNTLEESETIEYIQEIKDLDVYEQKDIDMVDKNKTLGVDIKVK
                     LPKSKLEGIESLSGGERALVSLALLFAIVAQSKPPLLVLDEVDAALDEENARRFGEML
                     QGLSKQT"
     gene            complement(687..2165)
                     /locus_tag="P5241_02985"
     CDS             complement(687..2165)
                     /locus_tag="P5241_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV32480.1"
                     /translation="MYLKEIELKGFKSFAKDTVIELVQGINAIVGPNGSGKSNIIDAL
                     RWALGDQSQRNLRVENSGDLIFTGNELCKSSSNAKVRLLFDDIKNTKTGLSCPLDCNT
                     LEIEKTIDSSGKTEYYLNKESVRLKDITEILASLQLGVKGFSIINQGSIENILKVSQE
                     ERAIMLEEILGLRQLEIKKEASLRKLSLTLINLDKVTSLENEILPHYRSLKKNVMRFE
                     KQKELKQMLKDYEKDYYSFKYKEITKNSVDLKAKLQEAQHKLNTIENNIKKEEEKLGL
                     ATTDEDEDKTNQFTYLRQTINEQIRIKNEEKNKLIYELAKTESILDNIPKYTFKPPET
                     KEFIIQDMPKIYSTIYKIRSLIQEIVPIDDIRVVRDKIKIIEQEISKILIDKTSNSVD
                     NTQEQVNLDIKELQNKIQEFKTKINTIERDLNMLYNQLEDIKQQEQEHNKNIKIAFAV
                     LEQQQHEKMIILNELKTLELSEEKIKINLENLKNQIGESVMV"
     gene            complement(2431..2970)
                     /locus_tag="P5241_02990"
                     /pseudo
     CDS             complement(2431..2970)
                     /locus_tag="P5241_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
     gene            complement(2992..3135)
                     /locus_tag="P5241_02995"
     CDS             complement(2992..3135)
                     /locus_tag="P5241_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32481.1"
                     /translation="MIDKAIDEANTKFVIENLEQKLNIKIKNPNLFLEALTHSSYLNE
                     NKH"
     gene            complement(3243..3677)
                     /gene="nusB"
                     /locus_tag="P5241_03000"
     CDS             complement(3243..3677)
                     /gene="nusB"
                     /locus_tag="P5241_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRV32482.1"
                     /translation="MDEIITRNINNYGSDLTDKEFLKKLVTGVVNYVKDIDAIIVKSA
                     PERPLHQINIIDRNILRIGLFEVLFEKSPDVPVKVAINEAVELAKNYGGDKAYSFVNG
                     VLGTVLRTVESIKDETKETEPETLTKIIQDQDNIVNKSSEVN"
     gene            complement(3791..4036)
                     /gene="rpmF"
                     /locus_tag="P5241_03005"
     CDS             complement(3791..4036)
                     /gene="rpmF"
                     /locus_tag="P5241_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRV32483.1"
                     /translation="MALPKTHHTKSKTHQRRSHMAKTKIQFTLCPKCHTTIKPHCVCP
                     NCGEYKGKIVIDVLKNLNKKEKKQKQKELKTSGKDKK"
     gene            complement(4270..4455)
                     /locus_tag="P5241_03010"
     CDS             complement(4270..4455)
                     /locus_tag="P5241_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32484.1"
                     /translation="MVGYDGLYYYLIPVESEKSALSFMDSDKSKVFLQDLAKRSDLEL
                     TKMIENGKIDIDNLTNP"
     gene            complement(4657..5586)
                     /locus_tag="P5241_03015"
     CDS             complement(4657..5586)
                     /locus_tag="P5241_03015"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRV32485.1"
                     /translation="MQNLLPFVQKTWPNAVVVPLIVCPKAVALPLDILESPNTFIIAS
                     VDFSHYLPKHLMELHDNKSIDNIVYFENLNNIDVDCPLCIKFLRDMAVKKGATNILEI
                     GRGNSFDYSQSKNPRTGTSYFSAIFSKEKTDTSNFYNINQTFLFVGDIMLARGVGDQI
                     DKYGFDYLLDNIKEAFRGVDYVVGNLEGPIVENAPKVLHGSFSFAFSKESVNFLKKYR
                     FNILSLANNHTNNYKGESGFVQTLDFLKSVGINSVGHYKDCSNKYIYNNNNDYFISFN
                     LTFGDNGCSQEIIKNIQNIKQTDNQSFVILMPH"
BASE COUNT         1892 a          976 c          634 g         2209 t
ORIGIN      
        1 tgagtttgtt tacttaatcc ttgtaacatt tcgccaaatc ttcgagcgtt ttcttcatcc
       61 aaggctgcat ctacttcatc aagtaccaac aatggtggtt tagactgagc aacaatagca
      121 aacaacaaag ccaaagacac caatgccctt tctcctcctg ataaagattc aataccttct
      181 aattttgatt ttggcagttt tactttaata tcaactccaa gtgttttgtt tttgtctacc
      241 atatctatat ctttttgctc atagacatca agatctttaa tttcttgaat atattctatt
      301 gtttctgatt cttccaaagt attcaaatta tttgatgatt gtacaatctc cacacttcct
      361 ttacctccac caaacaaaat ctgtaaatat ttattaaact ctttatttat ttcttttatg
      421 gaatcattaa attgtttatg aatttttata gtaagctctt taattatatg ctgtaaatca
      481 tcaacagctt gtttaagatc acttttttct ttttgcaaaa atgttaaacg ttcatctaca
      541 cttttgtatt cttctataat agaatcatca acaaatccca gctcttgtaa aatttttctt
      601 tgacgtaata ttttattttc taaattattt atttcttgct catcaaattg tttatcaaaa
      661 gtatctgcat agctcaaaaa ttcctctcaa accattacac tctccccaat ttgatttttt
      721 aaattttcta aattgatttt tattttttct tctgacaatt ccaaagtttt taattcattc
      781 aaaataatca ttttttcatg ctgttgttgt tccaagactg caaaggctat ttttatattt
      841 ttattatgtt cttgttcttg ctgttttata tcttctaact gattatacaa catatttaaa
      901 tctcgctcaa tggtattgat tttggtttta aattcttgaa ttttgttttg tagctccttt
      961 atatccaaat ttacttgttc ttgtgtatta tctacgctat tcgatgtttt gtctattaaa
     1021 attttggata tttcttgctc aattatttta attttatctc gcaccacccg tatatcatca
     1081 atcggaacaa tttcttgtat caagcttcta attttataga tagtagaata tattttgggc
     1141 atatcttgaa taataaattc tttagtttct ggaggtttga atgtgtattt tggtatatta
     1201 tctaaaatac tttcagtttt ggcaagctca taaataagtt tgtttttttc ttcgttttta
     1261 attcgtattt gttcattaat tgtttgtctc aaataagtaa attgatttgt cttgtcctcg
     1321 tcttcgtcag tggtagccaa acccaatttt tcttcctctt ttttaatatt attttcaata
     1381 gtatttaatt tatgttgagc ctcttgtaat tttgctttta gatcaacact attcttagta
     1441 atttctttat atttaaaact ataataatct ttttcataat ctttaagcat ttgttttaat
     1501 tctttttgtt tttcaaaacg cattacattt tttttcaaag aacgatagtg tggcaaaatt
     1561 tcattttcca aagatgtcac tttatccaaa tttataagag tcaaagataa tttcctcaaa
     1621 gatgcctctt ttttaatttc caattgcctt aatcccaaaa tctcttcaag cattatagct
     1681 cgttcttcct gacttacttt taaaatattt tcaatactgc cttgatttat aatagaaaat
     1741 ccctttacac ccaactgcaa agatgccaaa atttctgtta tatcttttag tcgcactgac
     1801 tccttgttta aataatactc tgttttacct gaactatcaa ttgttttttc tatttccaaa
     1861 gtattacaat ccaaaggaca cgataaaccc gtttttgtat ttttgatatc atcaaacaac
     1921 aatctgactt tggcattaga acttgattta cacagttcat taccagtaaa aattaaatct
     1981 ccactatttt caacacgtaa atttctttga ctttgatctc cgagcgccca acgcaaagca
     2041 tctataatat ttgatttacc agaaccatta ggaccaacta ttgcattaat cccctgtact
     2101 aactctatta ctgtatcttt ggcaaaagat ttaaagcctt ttaattctat ctcttttaaa
     2161 tacacaataa aaattttatc aaaaatgttt aaaaccccac aaacaaatct tgggcaatgt
     2221 ataatgtata ttatatattt tattatttct atacatattt cgactaaata taaatttgct
     2281 ccttatacca aaacacaaaa aatttataac catttaatat cgccatacca aacccagtgt
     2341 ttcctataca ttaagtgtca aatctttttg tattcatgat tgatcagcca aagctttggc
     2401 agctgcgtat tcttctgctt ctttttttga tcacccttct cctgttgcca attgtaaatc
     2461 ttttaaaaat actcctacta tatagcggat ctgatgagct ggcccttcgg aaaaaagagt
     2521 tttatattca ggagttatac cataaagttt ttgtgttaat tcttgaagca aactttttga
     2581 gtctttatga gtgtccaaac tcacaatagt ttccaaaaat ggctgtattg tagacattat
     2641 tactttttta gttgtttcaa aatctgtatc caaatataca gccccaaaaa cagcttcaac
     2701 agcatcagct aaactataac tctttatatt gatattcgga tctgtatttc gagccaacat
     2761 tttcaaaaat ttatccaaaa ccattcgctc tccttttgat acatataaag atttatccaa
     2821 atccaaatat aatgccacat tggaaagcat taatgagttt actaaattag aacgaagtgg
     2881 tgtcagcttg ccttcaggat aatcaggata ctttctaaaa atataatcag aaatacacaa
     2941 ttccaaaact gcatcgccca aaaattctaa tctttcatta caagcatacg gtcaatgttt
     3001 attttcattt agatacgaac tgtgggtcaa ggcttccaaa aaaagattag gattttttat
     3061 ctttatgttt aatttctgtt ccaaattttc tataacaaat ttagtattgg cttcatctat
     3121 cgccttatct atcataattg tataaattaa taaaattaaa aaaacaaata aatattatag
     3181 ccgaccaata tttcttattt cttattaaaa tttattataa tttattaaaa aaatgtatta
     3241 tcttaattaa cttcactgga tttatttaca atattatcct gatcctgaat tatctttgtt
     3301 aatgtttcag gctcagtttc ttttgtttca tcttttatac tttctactgt acgcaatacc
     3361 gtacctaaaa ctccattaac aaaactatag gctttatcac ctccataatt ttttgccaat
     3421 tctactgctt catttatagc tacttttaca ggaacatctg gagatttttc aaacaaaacc
     3481 tcaaacaatc caatacgtaa aatatttcta tcaataatat taatctggtg tagaggtcgc
     3541 tcaggcgcac ttttgacaat aattgcatct atatctttga cataattaac tactcctgtg
     3601 actaattttt ttagaaattc tttatcagtc aaatcagacc cgtaattgtt tatattacga
     3661 gttattattt catcaatatc ttttttttgt tctcaaaaat ctcattcata caaagattgc
     3721 aaagcaatca tacgagataa atgacgtgaa gccataataa tttttaaaaa aatattaaaa
     3781 aaataatctt ttacttttta tctttgccag aggtttttaa ttctttctgc ttttgtttct
     3841 tttctttttt attcaaattt ttcaacacat ctataacaat cttaccttta tattcaccac
     3901 aattaggaca tacacaatga ggctttattg tagtatgaca cttggggcac aaagtaaatt
     3961 gtattttggt tttggccata tgagacctcc tttgatgtgt tttggatttt gtatggtgtg
     4021 ttttaggtaa agccataata aaagtttaaa attaaactca aatcatatta tatttaaatg
     4081 tgtataactt aagtttaaca atttattaaa taaaaactaa tacaatacaa tacaatataa
     4141 aaagattatc aaaaaaacac taaaaaagca atatctctaa tcaattatga taaaatcaca
     4201 gtataatagg attgttggac aatgaagaaa ccagttttta tttttgagtt gcatattgtt
     4261 attttatatt taagggtttg ttaaattatc tatatcaatt ttaccatttt caatcatttt
     4321 tgtcaattct aaatcacttc ttttggcaag atcttgtaaa aataccttgc ttttatcact
     4381 atccataaaa ctcaaagcac ttttttcgct ttcaaccgga attaaataat aataaagccc
     4441 atcataacca accatcagtc cttgctgagt atttatcgaa aatcattgat caaaaataaa
     4501 attaccaagc gaataaaaaa ttattttatt tttatataat tcgatttctt gcacaacatg
     4561 aggatgagta ccaacaatta aatcagcacc agcatcaata aattgatgcg ctaatttttg
     4621 ttgtacacta ttagaatgtg ttttgtactc cgtgcctcaa tgaggcatta aaataacaaa
     4681 agattgatta tctgtctgtt ttatattttg aatatttttt ataatttctt gcgagcatcc
     4741 attgtcgcca aaagttaaat taaaactaat aaaataatcg ttgttattat tataaatata
     4801 tttattgcta caatctttat aatgaccaac actattaatt cctacagact tcaaaaaatc
     4861 taaagtttga acaaaaccac tttctccttt ataattatta gtatgattat tagccaatga
     4921 taaaatatta aatcgatatt ttttcaaaaa atttacagat tctttggaaa aagcaaaact
     4981 aaacgaacca tgcaaaactt ttggggcatt ttcaacaatg ggtccttcta aatttcctac
     5041 tacataatca acacccctaa aagcctcttt aatattgtct aataaataat caaacccgta
     5101 tttatcgatt tgatctccta cgcctcgggc caacataata tcaccaacaa acaaaaatgt
     5161 ctggttaata ttataaaaat tggacgtgtc agttttctct ttagaaaaaa tggcactaaa
     5221 atatgaagtc ccagttcgtg gattttttga ttgactataa tcaaaagaat ttcctctgcc
     5281 gatttccaat atattagtag ctcctttttt aacagccata tctcgtaaaa acttaataca
     5341 taacgggcaa tcaacatcta tattatttaa attttcaaaa taaacaatat tgtcaataga
     5401 tttattgtca tgtagctcca ttaaatgctt aggcaaataa tgcgaaaaat caacactggc
     5461 tataataaaa gtattgggac tttcaagaat atctaaaggc aaagccacag ctttggggca
     5521 aacaatcaag ggtacaacca cagcatttgg ccaagttttt tggacaaatg gtaataaatt
     5581 ttgtatacca tgttcctgac taatcgctca ataagcatta tctgttgtca aaaaagcact
     5641 tgaatataaa ctacaactat taaaatgatc tggacttaaa ataacaattt tatcataatt
     5701 aaatttggaa g
//
LOCUS       DAOTJA010000037         5598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13096_length_5598_cov_4.503518, whole genome shotgun sequence.
ACCESSION   DAOTJA010000037 DAOTJA010000000
VERSION     DAOTJA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13096_length_5598_cov_4.503518"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1154)
                     /locus_tag="P5241_03020"
     CDS             complement(138..1154)
                     /locus_tag="P5241_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRV32486.1"
                     /translation="MKYKVLNTELNINKWSLFVKNHPLGNIFQTSEMFLSYKNTKKYE
                     PIILSILNEKDEILGILLAIIQKEHSGLFGYFSARSITIGGPLVIDNNLEILDILLNE
                     YKKFIKGRAIYSQFRNLWEWGNEEKKVFLKYGFTFDSHLDIIISLENSSDELWKNLSR
                     DRKKSIKKGNSYLKTELIDFNEYFNRIYLLLKNVYRRIGLPLPEKMLFYNAMFYLGNI
                     NAIKTFGAFKDNKLVGVRIELCYNNLIYDWYAGADDSFLEYRPNDVLLWNIFLWGKDN
                     GFKYFDFGGAGKPNKPYGVRDYKLKFGGKLVNFGRFELVHKPIIYKFGIFALKNYKII
                     KKIL"
     gene            complement(1151..2359)
                     /locus_tag="P5241_03025"
     CDS             complement(1151..2359)
                     /locus_tag="P5241_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32487.1"
                     /translation="MNIIKISALNIALYTFFTLNPYFKWLLPNEYFYAFFLFTTLLLS
                     YQLKRYIKNSFHLDFHKKHIIFFLIIYIIYFTTPIVHSFRWGHLAWSIPFLIIITYSD
                     NIINLSYIYLKKIIIFFTITALIVWLINLLSIPIPYYSFYPNFRLISDDYYRIYGPCI
                     SLYNGSLPVGGVYGIERITGVFAEPGHFGIYLGLILAVEKFNLNSRENKILTLVGILT
                     FSTAFMGILLLGLLYRFYLEKKLIKIIPRILVYIITFVLLGLIFGEGFNKLMYERVFI
                     KDGKQINTLTELVESRINERSINEFDEFTKTSNYIFGVGYSEGVDMPTTNWRGVVYRF
                     GIIGLLIIIVLLITIVKRVDVLYAILLLAFAIIILVHRSYFFYVPSVYILLFIATNLN
                     KKLIIKHINI"
     gene            complement(2366..3559)
                     /locus_tag="P5241_03030"
     CDS             complement(2366..3559)
                     /locus_tag="P5241_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV32488.1"
                     /translation="MNKAINTLHLSSIGIWDLGQNKGRESTYLPLKGFVEKGHIVNFI
                     TIDKRHKTENINGINVEKVNLPIYPIPKKGFHNLTRMLLLPVTTLVLIIYVLCKYYKK
                     KPDVVYSHSTETTLAAYTIAKLFNSKYVLRMYGFPKFKKENIFHTLVRLDRTISLFLK
                     ADLYILTNDGTAADKAIESYGVPHYKVHFLKNGIDKSYLERPINHNLLKEIKPKNEKI
                     LLTASRLVDWKQVDLIIKALPFVLEKEKNIKLIIIGDGIERKNLETLAEKLNIRKFVT
                     FTGALEHNLVIDYIKLADIFISMNALSGMNNSVYEAMICGKVVIALNTGDTESLIKNN
                     ITGILLDPSKIEELPNIILDILSNNIKKQEIEKNAQNFMKEHWPTWNERINYEIDLIE
                     KLVNM"
     gene            complement(3561..4811)
                     /locus_tag="P5241_03035"
     CDS             complement(3561..4811)
                     /locus_tag="P5241_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRV32489.1"
                     /translation="MIKLVTGDSIARVIGLATAPIITRLYLPEHMGVLSVFTSLVALL
                     IPFGTFRYSLAIPLPKSDSSALNISVLSFILLTISTFFLFFIFIFFGNEILVFFSMEQ
                     VTHYWWLVPITFAGTGLYDLLSGWAIRKKEFKPLAKTKVWQKIIGDVVKVLFGLIDIR
                     PLGLLIGYILNQAGGILSLFKNFKIFFTDNVRCISRKRIIYFSKRYSDFPKYRIPSQF
                     LLNLTAKAPLLYFAWHFGADTTGQIGLATMMLSLPISLIGYTTGKAYYAEIATIGKRM
                     PEKIYKVTRNVSRKLFLFSIIPFSIILFFGPFIFKIVFGPKWNDAGVFASILSPYLLA
                     QFIYGPISEGILSVFEKQSKVLVIEISRTIITFLVFLISFILKSKPIYTLLLYSIGLT
                     LHYIFATIIVFLVIRRSKINNYII"
BASE COUNT         2036 a          871 c          719 g         1972 t
ORIGIN      
        1 tatatctgat attttaatgt caccactgat atacacatcc gaaccaacaa tgtaaccagc
       61 taatctcaag gttctaatgc gcattttatt tggaaaaaat ggcaacataa actttaaaat
      121 atgtttaagc atatttctca tagtatcttt ttaattattt tatagttctt taatgcaaat
      181 ataccaaact tatatattat tggtttatga actaactcaa atctaccaaa attaactagt
      241 ttgccaccaa attttaactt ataatctcta actccatagg gtttattagg tttaccagca
      301 cctccgaaat caaaatattt aaaaccatta tctttccccc ataagaatat attccacaac
      361 aaaacatcat taggcctata ttctaaaaac gaatcatcag caccagcgta ccaatcataa
      421 atcaaattat tataacaaag ttcgatcctc acaccaacta acttgttatc cttaaatgct
      481 ccaaaagttt ttattgcatt aatatttccg agataaaaca ttgcattgta gaataacatt
      541 ttttctggta ggggtaaccc aatgcgccta tacacatttt taagcaaaag atatattcta
      601 ttgaaatatt cattaaagtc aattaactca gttttgaggt aagaattacc ctttttaatg
      661 cttttttttc tatctcttga taaatttttc catagttcat ctgacgaatt ttctaaactt
      721 attattatat ctaaatgtga atcaaaagta aaaccatatt ttaaaaaaac ttttttttct
      781 tcgttacccc attcccacaa attacgaaat tgagaatata ttgctctccc ttttatgaac
      841 tttttgtact catttaataa aatatccaat atttccaaat tattgtctat aaccaaaggg
      901 ccacctattg ttatagatcg cgctgaaaag taaccaaata ggcctgagtg ctctttttga
      961 attatggcaa gcaatatacc taaaatttca tctttttcgt ttaaaataga tagtataata
     1021 ggttcatatt ttttggtatt tttatatgat aaaaacattt cagatgtttg aaatatgttt
     1081 cccaaaggat gatttttaac aaataaactc catttattaa tatttaattc agtattcaaa
     1141 actttatatt tcatatgtta atatgtttta taattaattt tttatttaaa ttagtggcta
     1201 taaacaatag aatgtatacg cttggcacat aaaaaaaata tgagcgatga accaaaatta
     1261 taattgcaaa agctagcaat aaaattgcat ataaaacatc tacccttttg acaattgtta
     1321 ttaataatac aattattatt aataatccaa taattccaaa tctataaaca actcctcgcc
     1381 agtttgttgt aggcatatca actccctcgg aataacctac tccaaatata taattagatg
     1441 ttttagtaaa ttcatcaaat tcgttaattg acctttcatt gattcttgac tctacaagtt
     1501 ctgtaagtgt atttatttgt tttccatctt taataaaaac tctttcatac attaatttat
     1561 taaatccttc tccaaaaatt aatcctaata atacaaaagt aataatataa acaagtattc
     1621 gaggtattat tttaatcaac tttttttcta aataaaagcg atataacaac ccaagcaaca
     1681 aaattcccat gaatgctgtt gaaaaagtta aaattccaac taatgttaat attttatttt
     1741 ctcttgaatt taaattgaat ttttcaactg ctagaattaa acccaaatat attccaaaat
     1801 gaccaggctc tgcaaaaact cctgtaatcc tctcaattcc gtaaacacct ccaactggca
     1861 aagaaccatt gtacaaacta atacaaggtc cataaattcg ataataatca tcacttatta
     1921 aacgaaagtt aggataaaaa gaataatagg gtattgggat acttagtagg tttattagcc
     1981 agacaattaa tgcagttatt gtaaagaaaa ttattatttt ctttaaataa atataactta
     2041 aattaatgat attatcactg taagtgataa ttatcaaaaa aggaattgac caggctaaat
     2101 gtccccatcg aaatgaatga acaataggtg tcgtaaaata aataatataa atgattagaa
     2161 aaaatataat atgtttctta tgaaaatcta agtgaaaact attttttatg tatcttttta
     2221 attggtatga taaaagcaaa gtagtaaaaa ggaaaaaagc ataaaaatac tcattaggta
     2281 aaagccattt aaaatatgga tttaatgtaa aaaaagtata taaagcaata tttagagcag
     2341 atatttttat tatattcatt tcgtattaca tgttaaccaa tttttcaatc aaatcgattt
     2401 cgtaattaat tctctcattc caagtaggcc aatgttcttt cataaaattt tgtgcatttt
     2461 tttcaatttc ttgtttttta atattattac ttaaaatatc taaaataatg ttaggtaatt
     2521 cttcaatttt actaggatct aaaaggattc ctgttatatt attcttaatt aaactttcag
     2581 tatctcctgt atttaatgca attactactt taccacaaat cattgcttca tatacagaat
     2641 tattcattcc acttaatgca ttcatgctaa taaaaatatc tgctaattta atatagtcaa
     2701 taactaagtt gtgttcaagt gctccagtaa aagtaacgaa ttttctaata ttcaattttt
     2761 cagcaagtgt ttctaaattt tttctttcaa ttccgtcccc tataataata agtttaatat
     2821 ttttttcttt ttcaagcaca aatggcaatg cctttataat taaatcaact tgtttccaat
     2881 caacaagtcg agatgctgtc aataatattt tttcattttt tggtttaatt tctttcaata
     2941 aattatgatt aataggtctt tctaaataac ttttatctat tccattcttt aaaaaatgaa
     3001 ctttgtaatg aggtacacca taagattcaa ttgccttatc agcagctgtt ccgtcattag
     3061 taagtatata taagtctgct tttaaaaaaa gtgatattgt tctatcaagc ctaactaaag
     3121 tatgaaaaat attttctttt ttaaattttg ggaaaccata cattcttaga acatatttag
     3181 aattaaacaa tttagcaatg gtatatgctg ccaaagttgt ttctgttgaa tgtgaatata
     3241 caacatctgg ttttttctta taatatttac ataatacgta aatgattaaa accaatgtag
     3301 tgactggtaa aagtaacatt cttgtcaaat tgtgaaaccc ttttttaggt attggataaa
     3361 ttggtaagtt aactttctca acatttatac cattaatgtt ttcagtttta tgccttttat
     3421 caattgttat aaaatttaca atatgtccct tctctacaaa tccttttaaa ggcaaataag
     3481 ttgattcgcg tcctttattt tgacctaggt cccaaattcc aattgatgaa agatgtaatg
     3541 tattaattgc tttattcata ctagataata taattgttaa tttttgatct acgtattact
     3601 aaaaaaacta tgattgttgc aaaaatataa tgcaaagtaa gcccgatact atataatagc
     3661 aaagtataaa taggtttaga ttttaaaata aatgatatta gaaatactaa aaaagtaata
     3721 atagttctac ttatttcaat tactaggact tttgattgtt tctcaaaaac gctaagaata
     3781 ccctcactta tcggaccata aataaattga gcaagtaagt agggtgacag aatacttgca
     3841 aaaactcctg catcattcca tttgggacca aatacaatct taaaaataaa ggggccgaaa
     3901 aaaagaatta tagagaaagg tataatacta aataaaaata attttcgtga aacattcctt
     3961 gttactttat aaattttttc tggcattctt ttgccaatag tagcaatttc ggcgtaatac
     4021 gctttgccag tagtgtaacc tataagggaa attggtaaag aaagcatcat agttgctaaa
     4081 cctatttgtc cagttgtgtc ggcaccaaag tgccaagcaa agtatagcag cggtgccttt
     4141 gcggtgaggt taagcaaaaa ctgtgatggt atacggtatt ttggaaaatc agaataccgt
     4201 ttactaaaat aaatgattcg ctttcgggaa atacacctta cattatcagt aaaaaatatt
     4261 ttaaaatttt taaacaagct aaggatacct cctgcctgat taaggatata ccctattaat
     4321 aggcctaacg gtctaatgtc aataaggcca aatagaactt taacaacatc tccaataatt
     4381 ttttgccaaa ccttagtttt tgctagaggt ttaaactctt ttttacgaat agcccaaccc
     4441 gacaatagat cgtataatcc tgtaccagca aaggtaatgg ggacaagcca ccaataatga
     4501 gttacttgtt ccattgaaaa gaacacaagt atttcattcc caaaaaaaat aaaaataaag
     4561 aagaggaaga atgtagatat agttaaaaga ataaaagaaa gaacagaaat atttaaagca
     4621 gaactatcac tttttggaag tgggattgcc agtgaatacc taaaggttcc aaagggaatt
     4681 aataatgcca ccagtgatgt aaatactgat aaaactccca tgtgctctgg gaggtaaagt
     4741 cgtgttataa taggtgccgt tgctaatccg ataactctag caatactatc gcctgttact
     4801 agcttgatca tattgctaaa aataacacct tcatgggttt gcttatccca acttttagat
     4861 agcttttgcc aaatattttt tgttatttta gaaaatacca ttcgcgaata atgatatgta
     4921 attcattaaa atatatttat ttaatttaaa ttaattatta ttaaaataaa atatcaatgc
     4981 tgaaaatact tagcagaata tcacataaat cacttaatac taatattcta gatagagtca
     5041 aagagtaagc ggttgggggg tatgttaact cagaatactg aagtcaatgt tgttaaaaaa
     5101 gttttatttt cattttaagt aaactttccc gtacaactta tagctaaact tctatttaat
     5161 ttcatcaaaa atgagatcct tagatttgcg ctaatgtaaa gtgagcgctt tggatgtcta
     5221 tatcattagg ttctcttttt aactaagcaa cattgaatct tgaattttta ttcacgagtc
     5281 aatcatctct ctgtacactg cactctgaat actataaact aaatacagct tcgccctgtc
     5341 agttacaggt ttacaacctg caaaccaaaa tacatatttg tgctgaggct gtatatgttt
     5401 tagtttaatt aagcaaccga atatttgaag ggtggagaga tttttcggct atgctcaaaa
     5461 tgacaaattc agtcaaaatg aaaaaaggaa cagcctcaat tttttaaaat aaccgtttaa
     5521 tacgctcctt ccaggttggt tgcggttcat cgtcgtcgga gtagtagccg ctgccggtat
     5581 aggggccgta gccatacc
//
LOCUS       DAOTJA010000038         5523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13290_length_5523_cov_3.997805, whole genome shotgun sequence.
ACCESSION   DAOTJA010000038 DAOTJA010000000
VERSION     DAOTJA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13290_length_5523_cov_3.997805"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1990
                     /locus_tag="P5241_03040"
     CDS             275..1990
                     /locus_tag="P5241_03040"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRV32490.1"
                     /translation="MRLSKAFIPTLKEVPADAVITSHKLMIRSGMIRPLTAGVYSFLP
                     LGYRVIKKIIDIVREEMDSIGAQEFFLPALNPIELWEQTDRVKAFGDTMYHVKNRPYV
                     LAPTHEEVIAHIAKNHIQSYKDMPQVWYQVQTKFRNEARPRSGVIRSRQFFMKDAYSL
                     DSSWEGLDKSYNDQHEAYKKIFTRCGIKFFVVGASSGAMGGNKSQEFMVESNAGEDTC
                     ALCDKCGYAANVEVATSKKEKIGKRGESKSIEKIHTPNIKTIDELCSFLKITPDDCAK
                     AVVYFYQVEDKWEPVLIFMSGNDELNESKLTSVLGCQVVPAKPEELFDIFGANAGSLG
                     PINIKKKIKIIADKRLEGANNLYSGANEDDYHLSGIDFERDVKVDGYYDLRTVIEGEA
                     CPDCGTPLRIVKAIELGHIFKLGTKYSEGLQANFLDEKGEEKPIIMGSYGIGIERVCA
                     CYIEQNNDEYGIIWNKALAPYLVSLIAVNSNVKEVMQKSEEIYNLLVKKNIDTVYDDR
                     VGVSPGFKFKDADLIGIPLQVLVSEKNLKNGKIEIKIRRTGERKLFDQDKIIESIENI
                     LKEID"
     gene            2193..3347
                     /locus_tag="P5241_03045"
     CDS             2193..3347
                     /locus_tag="P5241_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32491.1"
                     /translation="MSNESSKIINRINYIDQLRAWNVLLMCYTHSLDAMLSDEYKTGI
                     FFKIIHFIEGIIAPSFLFVSGAAFAIVLHKRIDGYLNYGKPAQKQLLRLLFLLLIAYS
                     LNLPYKTLNQCNTILTFEQYLLFIKSNNLHVIVFGIFFSQILFLIIRNEKKFHWVLFF
                     LSILVVSVTPTIYQYDFGKVLPLPLATLINTKYLSAFPLFHWIAYYFIGAFVMFQLIR
                     AIQENREEDLIKNLFFVAIFSSFIFTLLEIIGIETTSYYDFWLTSPNIFIIRLSSVLV
                     LVCLFYYLETRFNYRMIVFNVFKSESLLVYVLHLMIIYGYVLAPGIDKIFGKTLNWAE
                     IIGLGTIISATLLAIAYMWNWFKKNYKLHSRIFLTLIWLYFIYYFLTKPY"
     gene            3372..4583
                     /locus_tag="P5241_03050"
     CDS             3372..4583
                     /locus_tag="P5241_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRV32492.1"
                     /translation="MKQYFSNLFINLIKKINLKLDKNQNKQKDRIEYIDFLRASAVVL
                     MIFAHVLDATLSPEFKKDNLYRLMNFINGFVAPAFLFAAGASFTIIITKRKNDIISFQ
                     KPFFKQLWRVIQLLAIGYFLHLPYKTLNQMNTIITRDEYVNFVRSDILHVIAFGLLIS
                     LIVLIIFRKEKRFYLVIFIISLLTISLTPLFRNITFGDNLPIEIATFMNKQYNSIFPL
                     FPWLAYLFIGSIVMYYIIKYSREGTIEDIFKKLFFLCIILISLSAIPELIGIQPSDKY
                     NFWHTSVNIVCIKLCVVILYMLLMWKLENKYSYKMKIVKIFGKESLFVYVFHLIIVYG
                     SIFSIGLNKSIGRNLDRVEMFLVFLGVVIVSLIATYIWNYIKNTSLLLTRILLFLFVG
                     YFAYYFLTKPF"
     gene            4588..>5523
                     /locus_tag="P5241_03055"
     CDS             4588..>5523
                     /locus_tag="P5241_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRV32493.1"
                     /translation="MKRIKYIDILRGWAVLLMVFWHMTEALISDSIRLTSFFDISQFI
                     GGLVAPMFLFSSGASHSIIILKKRESFIKFTPNLRKRIIRIFQILIIAYLLHLPENNL
                     LEVIITRQGSSYINFINMDVLQLIAVSLLLLQILFLIIKNNKIYFYIVGIIGVITILL
                     TPYIWQINFSDFLPVELASCLNNKTGSLFPIFPWLSYVFLGAMVMQFVMEKGEENTIK
                     KMLILGFIIILLGLVPQLLNLKSTPNYDFWQTSPNIFLIKFGIVLVFLSIFYFMDKFK
                     NYEMKYFGVFGKESLFIYVVHLLMIYGHNFYTLQTN"
BASE COUNT         1923 a          594 c          913 g         2093 t
ORIGIN      
        1 aataaagatt tttggtatac aataaaatta cttttttaaa ataaaaaccc tttatgtttt
       61 ttcataaagg gttttttgtt tttatataag agttagtaat tttaactcaa atttaaaata
      121 ttttacttta caatttgttt ttagttataa tttagtggtt tataatatgt gttacataga
      181 aagttatgat tgtaaaaaat atattgcatt tgaataaaaa tttttgtata aatataaatt
      241 atgtttttat ttagaatttt atgagagaaa aagtatgagg ttaagtaaag cttttattcc
      301 aacattgaaa gaggttcctg cagacgcagt aataacaagt cataagttaa tgatacgttc
      361 gggtatgatt aggccattaa ctgctggtgt atattcattt ttaccattag gatatcgtgt
      421 tattaagaaa attattgata tagttcgtga agaaatggac tcgataggtg cacaggaatt
      481 ctttttgcct gcactaaacc caatagaatt atgggaacag acggatagag taaaagcttt
      541 tggtgataca atgtatcacg taaaaaatag gccttatgta ttggcaccaa ctcatgagga
      601 agtgattgca catattgcaa aaaatcacat tcaatcatat aaagatatgc ctcaagtatg
      661 gtatcaggtt caaacaaaat tcagaaatga agcaagacca agatctggcg tcataagaag
      721 ccgtcagttt tttatgaaag atgcttattc tttagattca tcgtgggaag gattagataa
      781 aagttataat gatcaacacg aagcttacaa aaaaatattt acacgatgtg gaattaagtt
      841 ttttgttgtt ggcgcttcaa gtggtgcaat gggagggaat aagtcacagg aatttatggt
      901 tgaatctaat gccggagaag atacctgtgc tttgtgtgat aaatgtggtt atgcggcaaa
      961 tgttgaagtt gctacttcaa aaaaggaaaa aattggaaaa agaggagaaa gtaaaagtat
     1021 agagaaaata catactccaa atataaaaac aatagatgaa ctttgttcat ttttgaaaat
     1081 tactcctgat gattgtgcta aagctgttgt atatttttat caagttgaag ataaatggga
     1141 acctgtttta atttttatgt caggtaatga tgaattaaat gaatcaaaat taacttcagt
     1201 acttggctgt caagtagtac cagcaaaacc agaagaactt tttgatattt ttggagctaa
     1261 tgctggttcc ttgggtccta taaatatcaa gaaaaaaata aaaataattg cagataaaag
     1321 gttagaaggt gcaaataacc tttatagtgg tgcaaatgaa gatgactatc atctttcagg
     1381 aattgatttt gaaagagatg taaaagtaga tggctattac gatcttagaa ctgtaattga
     1441 aggtgaagct tgtccagatt gtggtacacc tctaagaatt gttaaagcta ttgaacttgg
     1501 ccatattttt aagttaggta caaaatactc tgagggctta caagctaatt ttcttgatga
     1561 aaagggggaa gaaaagccta ttataatggg atcttatggt attggcattg aaagagtttg
     1621 tgcttgctat attgaacaaa ataatgatga atatggaatt atatggaata aggctcttgc
     1681 tccatacctg gttagtttga ttgcagttaa ttctaatgtg aaagaagtta tgcaaaaatc
     1741 tgaagagata tataatttat tagttaagaa aaacattgat acagtttatg atgatagagt
     1801 tggggtaagt ccgggcttta agtttaaaga tgctgatttg ataggtatac ctttgcaggt
     1861 tttagtgagt gagaagaatc tgaaaaatgg taaaatagaa ataaaaattc gaagaacagg
     1921 agaaagaaag ctttttgatc aagataaaat aattgagtca attgaaaata ttttaaaaga
     1981 aatagattaa ttttttttta agaacaggta tatagtgtat gcctgttctt ttttgttttg
     2041 aataatgtgt caaaatatta ttgattataa aatatttttt gtgtaaatta ttgagttaat
     2101 atgtttttgg ttaggtagtt ttgtttaatg ataggattat gtagtattgt gtttttcccg
     2161 ccaaatttga gttctcaaat agataggata aaatgagtaa cgaatcatct aagattatta
     2221 atcgtatcaa ttatattgat caattgagag catggaatgt tttgctaatg tgctatacac
     2281 attcattaga tgcaatgcta agcgatgaat ataaaacagg tatatttttt aaaataatac
     2341 attttattga aggaataata gccccatctt ttctttttgt atccggtgct gcatttgcta
     2401 ttgttttaca taaaagaatt gatggttact taaattacgg taaacctgca cagaagcaat
     2461 tgttaagatt acttttttta cttttaatag catactcttt gaatctaccc tataaaacac
     2521 taaatcagtg taatacgatt cttacatttg aacagtattt gctgtttata aaatcaaata
     2581 atttacatgt aatagtattt ggaatttttt tctctcaaat tctttttttg attataagaa
     2641 atgaaaaaaa atttcattgg gttttattct ttttatcaat acttgttgta tcagtaactc
     2701 caacaatata tcaatatgat tttgggaaag ttttaccatt accactagca acactaataa
     2761 atacaaaata tctttctgct tttccattgt ttcattggat tgcttactat tttattgggg
     2821 cgtttgtaat gtttcagtta atacgagcaa ttcaagaaaa tcgagaagaa gacttgataa
     2881 aaaacttatt ttttgttgca atattttcga gttttatctt tacactatta gaaatcattg
     2941 gaatagaaac aacatcttat tatgattttt ggttaacgag tccgaatatt tttattataa
     3001 ggctaagttc tgttttagtt ttagtttgtc ttttttatta tctggaaacg agatttaatt
     3061 acaggatgat agtttttaat gtatttaaaa gtgaatcact cttagtctat gttttacatt
     3121 tgatgattat ttatggttat gtattggctc caggaataga taaaatattt ggaaagactt
     3181 taaattgggc tgaaattatt ggtcttggaa caattatttc agctactttg cttgcaattg
     3241 cttatatgtg gaattggttt aaaaagaatt ataagctgca ctcaagaatt ttcttaacat
     3301 tgatatggtt atacttcatt tattatttcc taaccaaacc ttattagctt ttcacaaaat
     3361 taaaacaatg tatgaaacaa tattttagta atttatttat taatttaata aaaaaaatta
     3421 atctaaaact tgataaaaat caaaacaaac aaaaggatag aatagagtat attgattttc
     3481 tacgagcatc tgctgtagtg cttatgattt ttgctcatgt acttgatgca acgttaagcc
     3541 ctgaatttaa aaaagataat ttatatcggt taatgaattt tataaatggg tttgtagccc
     3601 ctgcgttttt atttgctgcc ggagcatctt ttacaatcat aataacaaag aggaaaaatg
     3661 atataatcag ttttcaaaag ccatttttta aacaattgtg gcgtgttatt caacttttag
     3721 caattgggta ttttttacat ttgccatata aaactttaaa tcaaatgaac acaataatta
     3781 caagagatga atacgtaaat tttgtaagaa gtgatatact tcacgttatt gcttttggtt
     3841 tattgatttc tcttatagtg ttgataattt ttcgcaaaga aaaaagattt tatcttgtga
     3901 tatttataat tagtcttttg acaatatctt taacaccact atttagaaat attacctttg
     3961 gtgataatct accgattgaa attgctacat ttatgaacaa acaatataat agtattttcc
     4021 ctttatttcc atggttggca tatttattta ttggttctat agttatgtat tatattataa
     4081 aatattcaag agagggtact atagaagata tttttaagaa attatttttt ttatgcatta
     4141 ttttaatatc tctttcagct atacctgaat taataggaat tcaacctagt gataagtata
     4201 atttttggca tacaagtgtt aatattgttt gtatcaaatt atgcgtagta attttatata
     4261 tgttattgat gtggaaatta gaaaataaat atagttataa aatgaaaatt gttaagatat
     4321 ttggtaaaga atctcttttc gtttatgtat ttcacttaat aatagtatat ggttctattt
     4381 ttagtattgg tttaaataaa agtattggta gaaatttgga tagggtggaa atgtttcttg
     4441 tttttttagg tgtggttatt gtgtcactta tagctacata tatatggaat tatattaaaa
     4501 atacttcttt acttttgacc agaatactat tatttttgtt tgtaggatac tttgcatatt
     4561 actttttaac aaaaccattt taattaaatg aaaagaataa aatatattga tattttaaga
     4621 ggctgggcag tactcttaat ggtattctgg cacatgacag aagcattgat atctgatagt
     4681 ataaggttga catcattttt tgatattagt caattcatag gtggtttagt agctccaatg
     4741 tttttgtttt cgtcaggtgc ttcacattca ataataattt tgaagaaaag agaaagtttt
     4801 ataaaattta cacctaattt aagaaaaagg ataattagaa tatttcaaat tttaataata
     4861 gcttatcttt tgcacttacc agagaataat ttattggaag taataataac aaggcaaggg
     4921 agtagttata ttaattttat caatatggat gtattgcaac tgatagctgt tagtttgcta
     4981 ttgctacaaa ttcttttttt aattataaaa aataataaaa tatactttta tattgttggt
     5041 attattggag tgataacaat attattgaca ccgtatatct ggcaaattaa tttcagtgat
     5101 tttttaccag ttgagcttgc ttcttgcttg aataataaaa ctggttcact tttcccgatt
     5161 tttccatggc tatcttatgt gtttttagga gctatggtaa tgcaatttgt tatggaaaaa
     5221 ggagaagaaa acacaataaa gaaaatgtta atattgggtt ttataataat tcttttaggc
     5281 ttggtgcctc aattgttaaa tctgaaatca accccaaatt atgatttctg gcaaaccagc
     5341 ccaaatatat ttttaattaa gtttggtatt gttttagtat ttttatcgat cttttatttt
     5401 atggataaat tcaaaaatta tgagatgaaa tattttggtg tatttggcaa agaatcactg
     5461 tttatttatg ttgttcatct tttaatgata tatggacata atttttatac tctacaaaca
     5521 aat
//
LOCUS       DAOTJA010000039         5492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13364_length_5492_cov_5.553982, whole genome shotgun sequence.
ACCESSION   DAOTJA010000039 DAOTJA010000000
VERSION     DAOTJA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13364_length_5492_cov_5.553982"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    62..161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(445..579)
                     /locus_tag="P5241_03060"
     CDS             complement(445..579)
                     /locus_tag="P5241_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32494.1"
                     /translation="MESKYIEVILHSSPISDEDPSVVYKDGLFKSKGLIVGTVVLLSP
                     "
     gene            569..2053
                     /locus_tag="P5241_03065"
     CDS             569..2053
                     /locus_tag="P5241_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32495.1"
                     /translation="MDSKQPIYEIGDTIKGTIRSSCADKLKINKYTIQFLPEQGDKGI
                     TISSTDGKFEQRYDINIITILAKAGQSGKTKVKIVAVGNYENAKGEAKITAPKEIYIT
                     VDAGSTEGDTTPTGGKTIKIAVDKKEYNKLDDTIKLTIVSKPEGLVDCGYNLEADYQN
                     TGKYGYKVEGIEIPNCTGSINIPVNKAYKMKLSVAGYIRKGNKTTTYESDPQFIELVY
                     KPGAKTDTSKIKNDCYLDIVSMTPNTNKIYVTNQQTQDITVKFKLVCTDYSTIDVKPT
                     KVSAYIDIHDLYNEGKKQSVLCQSQNMNAEVYKSGTEYTLSCSAKTLATLPNDARLKL
                     LMVDMYYLTNQEDGFHHGEEHTERFISTRDKAIQIVTNENKGGDKGGGAEGKPSPEQQ
                     KEIIHLSVNPTIAYENQELTFTITSDYKPQANEAKCMIYWKNSDQKEGNFISNYSLDN
                     LLTTKIFKAKKDGAQQTYIQVRCADATIATKYYSNTVTVHLAES"
     gene            complement(2080..2631)
                     /locus_tag="P5241_03070"
     CDS             complement(2080..2631)
                     /locus_tag="P5241_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32496.1"
                     /translation="MQASCTPPPDSQLNITDSPFLILVLSAFNFTQAPSFDSSGLVSV
                     GLVSGGLVVCFVNTISWVVNNQLQLTFLLVFACSSSKVLQTSNVALTLALLYVFNVFS
                     MLVLVWAGNLLQRLDIAYSLIPESGIVISWIIVLSIFIRANVFWNLLRLPLPTTFIVK
                     LSELIGLLFSYFRLICFVISNVN"
     gene            2756..3214
                     /locus_tag="P5241_03075"
     CDS             2756..3214
                     /locus_tag="P5241_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32497.1"
                     /translation="MNELIDNIDDKGGEEKFREYVKDDKNRGIVFSNNVKIEVLSSKE
                     PEKSTIQGQIPTGCSKVSISDDITNKSIPSASVQLVNKDNKYSGLAKVNAYIDVPGYS
                     TIFLPDWNSNGAKGITNLVYPFNLNTSSALKELAKIPSSKINQATLTINV"
     gene            3239..3409
                     /locus_tag="P5241_03080"
     CDS             3239..3409
                     /locus_tag="P5241_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32498.1"
                     /translation="MDLCNRHKFTNKGGEITPSTKPPEPKKATNCSTICNQAPSITFG
                     LMGLGAQSSFVS"
     gene            3449..3808
                     /locus_tag="P5241_03085"
     CDS             3449..3808
                     /locus_tag="P5241_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32499.1"
                     /translation="MSRLCVRVNYKNCNNQAIFNRGIALKVNNSLIKTQTFTPDNYTM
                     YYLASQTGAIDICSGTVKELEQLGELKFVPDKLHFATLKLSADTTLCTDKNCSLYCFY
                     ENSVQKKVNLTSIEQQR"
     gene            3975..4667
                     /locus_tag="P5241_03090"
     CDS             3975..4667
                     /locus_tag="P5241_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32500.1"
                     /translation="MFESKIPEMPYSKSEKTESKKTDLDPESHKIEHISKTETKPKAI
                     TDKNTDKKKNIEAQLKEILEQERRDFGEPPYQIKEQTTEQNHTYPVLETKKQNLDFDS
                     LDKLFHKTFVPSSSEKTVFNDLSLLQKQEKQLRKINKKDKIVVVDNTESLLEWIKQTK
                     IKGMDAVVAIGCRFADKGDEPSYLAKQVAETSAQLEKISKELVLSSGKEKIGTSITEA
                     ESLYEYIEQNLK"
     gene            complement(4793..4927)
                     /locus_tag="P5241_03095"
     CDS             complement(4793..4927)
                     /locus_tag="P5241_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32501.1"
                     /translation="MSKEKEATLFNDFSILAGTIKTPISLSGNNFLQRKENVFAKRFK
                     "
BASE COUNT         2156 a          756 c          868 g         1612 t
ORIGIN      
        1 ggttataata caactcctgg tgcagcaaat cagtgatcta cttctggtgg ttataataca
       61 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctcctggtg ccaatcagtg
      181 gtctacttct ggtggttata atacaactcc tggtgccaat cagtggtcta cttctggtgg
      241 ttataataca actcctggtg cagcaaatca gtgatctact tctggtggtt atactacaac
      301 tcctggtgca gcaaatcagt gatctactac aggaggcaca ggcaatcaat ttcaaacaac
      361 tcccggtaca ggcacaggta atctctgaac aggatttttt aatagtataa aagatttatt
      421 aggtattcct acaactaata atactcaagg tgataataaa actacagttc ctactattag
      481 tccttttgat ttaaataatc catctttata tactacagaa ggatcttcat ctgatatcgg
      541 agatgaatgt aaaattactt ctatatactt ggattccaaa cagccaatat atgaaatcgg
      601 cgatacaata aaaggcacca ttcgttcttc ttgtgcagat aaattaaaaa ttaataaata
      661 tactattcaa tttttgcccg agcagggaga taaaggtatt actatttcaa gtactgatgg
      721 taaatttgaa caaagatatg atattaatat aattactata ttagctaaag ctggacaatc
      781 tggtaaaaca aaagtaaaaa ttgttgcagt aggtaattac gaaaatgcta aaggtgaagc
      841 taaaattact gcacccaaag aaatttatat tacagttgat gctggaagta ctgaaggaga
      901 tactacacca acaggtggta aaacaattaa aattgctgtt gataaaaaag aatataacaa
      961 gttagacgat acaataaaat taactattgt atctaaacca gaaggacttg tagattgtgg
     1021 atataatctc gaagctgatt atcaaaatac agggaaatat ggttataaag tagaaggtat
     1081 agaaatcccc aattgtacgg gtagtatcaa tatccccgtg aataaagctt ataaaatgaa
     1141 attaagtgtt gctggttata ttcgtaaagg aaacaaaact actacttatg aatctgatcc
     1201 tcaatttata gaacttgttt ataaacccgg agcaaaaaca gatactagca aaattaaaaa
     1261 tgattgttat cttgatattg ttagtatgac acccaataca aataaaattt atgttaccaa
     1321 tcagcaaact caagatatta ctgttaagtt taaattagtt tgtaccgatt atagtactat
     1381 tgatgttaaa ccaacaaaag taagtgctta tattgatatc catgatttat acaatgaagg
     1441 caaaaagcaa tctgttttat gtcagagcca aaatatgaat gctgaagttt ataaatctgg
     1501 tacagaatat actttgagtt gttctgccaa aaccttagct actttaccaa acgatgccag
     1561 attaaaactt ttgatggtag atatgtatta tctcaccaat caagaagacg gatttcatca
     1621 tggagaagaa catactgaaa gatttatttc taccagagac aaagctatac aaatagttac
     1681 taatgaaaat aaaggaggag ataaaggagg aggagcagaa ggaaaaccaa gtccagagca
     1741 acaaaaagaa ataattcatc tttctgttaa tcctactata gcttatgaaa atcaagagct
     1801 tacttttact attacttcag attataaacc tcaggctaat gaggcaaaat gtatgatata
     1861 ttggaaaaat tctgatcaaa aagaaggtaa ttttatctcg aattatagtt tagataattt
     1921 acttaccact aaaatcttta aagccaaaaa agatggagct caacaaacat atattcaagt
     1981 tcgttgtgct gatgcgacaa ttgctacaaa atattatagt aatacagtta cagttcattt
     2041 agccgaaagt tgaggagaaa tatgtaaagc taatccttgt caatttacat ttgatattac
     2101 aaaacaaatt aatctaaaat atgaaaacaa taaaccaata agctctgata atttaacaat
     2161 gaaagttgtt ggtaagggta atctcaacaa attccaaaaa acatttgctc gaataaaaat
     2221 tgataataca attatccaag atataactat cccagattct ggtataagag aatatgctat
     2281 atctaatctt tgaagtaaat taccagccca aactaaaacc aacatagaaa atacattaaa
     2341 gacatacaat agtgccaaag ttaatgccac attagaagtt tgaagcactt ttgacgaaga
     2401 acaagcaaag actaacaaaa aagttaattg taattgatta ttaacaaccc aagatattgt
     2461 gtttacaaaa caaacaacta aaccaccaga aactaaacca acagatacta aacctgaaga
     2521 atcaaaagaa ggggcttgag taaaattaaa agctgataaa actaaaatta aaaaaggtga
     2581 atcagttata ttcagttgag agtcaggggg aggagtacac gatgcttgca ctttagactg
     2641 ttatgatggt gctggggagt taataattgc atgagccggg ttaggcaaaa cagataaaaa
     2701 agaaataatt ccagaagaaa attgtgtttg tagagtacac tgtatctcgg aagaattgaa
     2761 cgaacttata gacaatatag acgataaagg aggggaggaa aaatttagag aatatgtaaa
     2821 agatgataaa aaccgtggaa tcgttttcag taataacgtg aagatcgaag ttttgtcgtc
     2881 aaaagaacca gaaaaatcga caatacaagg acaaattcct actggctgtt ccaaagtatc
     2941 aatttctgac gatattacaa ataaatctat tccaagtgct agtgtacaat tagtaaataa
     3001 agataataaa tattcaggat tagcaaaagt taatgcttat attgatgtac caggatactc
     3061 aactattttt ttgccagatt ggaatagcaa tggagctaaa ggtataacaa atttagtata
     3121 tccatttaac ttaaatactt ctagtgcctt aaaagaatta gcaaaaattc catcgtctaa
     3181 aataaatcaa gctacactga caattaatgt ttgagctttt accaaagatg gtggttttat
     3241 ggatttatgt aatagacata aatttactaa caaaggggga gaaataactc ccagcacaaa
     3301 acctccagaa cctaaaaaag cgacaaactg tagtacaata tgtaatcaag ctccgtcaat
     3361 aacttttggt ttaatgggtt tgggtgccca gagttctttt gtatcctgag agaatttacc
     3421 aaaagatctt acgtataata atatactaat gagccgtttg tgtgttagag taaactataa
     3481 gaactgtaat aatcaggcta ttttcaatag gggtatagct ttgaaagtta ataattcgtt
     3541 aataaaaaca cagaccttta cgccagataa ttatactatg tattatcttg caagtcaaac
     3601 tggtgcaatt gatatttgta gtggtacagt aaaagaatta gaacaattag gagaattaaa
     3661 atttgttcct gacaaactcc attttgcaac gttaaaacta tctgctgaca ctactttgtg
     3721 tacagacaag aactgctcgt tgtattgttt ttatgaaaat agcgtacaaa aaaaggtaaa
     3781 tttaacttcc atagaacaac aaagataaat tagataaaga agatgaaaga aacaactcta
     3841 tttttggtat tttattagtt tcatcgttat tttgtttaaa atctgggctg gctaaaagcc
     3901 agcccagaat gcagacttgt atttttatta aaatttgtgt ataattagaa taatttaaaa
     3961 attaaatttt caatatgttt gaatcaaaaa taccagaaat gccttattcc aaatccgaaa
     4021 aaactgaatc caaaaaaact gatcttgatc cagagtcgca taaaattgaa catatttcca
     4081 aaaccgaaac aaaacccaaa gcaattacag ataaaaatac agataaaaag aaaaacatag
     4141 aggctcaatt aaaagaaata ttagaacaag aaaggcgaga ttttggcgag cctccatacc
     4201 aaattaaaga acaaaccaca gaacaaaacc atacttatcc tgttttagaa accaaaaaac
     4261 agaatttaga ttttgatagt ttggataaat tatttcacaa aactttcgta ccttcttctt
     4321 cagaaaaaac ggtttttaat gatttatcat tattacaaaa acaagaaaaa caattaagaa
     4381 agataaacaa aaaagataaa attgtagttg ttgataatac agaaagttta ttagagtgga
     4441 ttaaacaaac aaaaattaaa ggaatggatg ctgttgtagc tattggttgc cgatttgctg
     4501 ataaaggaga cgagccatct tatttagcta aacaagtggc tgaaactagt gctcaattag
     4561 aaaaaatatc taaagaatta gttttatcaa gtggtaaaga aaaaatcggt acttccatta
     4621 ctgaagccga aagcctatat gaatatattg aacaaaattt gaaatgacct caaattattt
     4681 attttgaaac tcaaagtaaa aacacatcta ttgaccaatg ttttcgttta atagaaatgt
     4741 gttatcaaaa caattgagat agaattacta tagttaatat agctgataaa ttttatttaa
     4801 aacgttttgc aaaaacattt tcttttcttt gtaaaaagtt attgcctgat aaagaaatag
     4861 gtgtttttat tgttccggct agtattgaaa aatcattaaa taatgttgct tctttttctt
     4921 tcgacaaact attttctcaa aaatgaagct ctgatattga ttgaaaatga atgaaaaaac
     4981 gtgaaggctt taatttgtgg aatattggtt cgtctgtagc aactgatata ttattacatt
     5041 ctggtgcttg aaaaaagatg tgttatacaa aagataaaca aaacatagat aaataaaatt
     5101 tgacaaaaat agaaaaattt gctaaaataa taaacaatct ctaccaaggt gaaattgcaa
     5161 aacagccctc gaaagggggc tgttttgttt tgataaaaat agagttaatc aaaaagaatc
     5221 tgatagaaca gaaaaatgaa ttaatttgtt ttaacatttt gatatacaga tatatttgta
     5281 caaaataaaa acagaaaatc aaaacaggca ggaaaatttc tgcctgtttt gattttttct
     5341 taattatgtg ttaatataat aatatatatg aaatttagaa aaaatttagg aaatggaaaa
     5401 tcatcacaga cttatttgag tagatattgt tcgaggcata ggaataattt ttattatttt
     5461 aactcatatg ccgattaata gaaatctgac tg
//
LOCUS       DAOTJA010000040         5452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13470_length_5452_cov_6.361312, whole genome shotgun sequence.
ACCESSION   DAOTJA010000040 DAOTJA010000000
VERSION     DAOTJA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13470_length_5452_cov_6.361312"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..401
                     /gene="rpsT"
                     /locus_tag="P5241_03100"
     CDS             135..401
                     /gene="rpsT"
                     /locus_tag="P5241_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRV32502.1"
                     /translation="MPIIKSAKKALKQSKKRHISNLNYKQQIRHLKKEIEKSIKAGKT
                     AEVEELIKKFYKATDKAAKEHVIHKNKAARLKSKAVKKIQRSHS"
     gene            682..885
                     /locus_tag="P5241_03105"
     CDS             682..885
                     /locus_tag="P5241_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32503.1"
                     /translation="MQNNGISLDRVNNYIHFMITFDNGEMLALSDLRKFAKIIVGKKQ
                     DIESLKELVSLGKDPFDKTFTKT"
     gene            898..1269
                     /locus_tag="P5241_03110"
     CDS             898..1269
                     /locus_tag="P5241_03110"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRV32504.1"
                     /translation="MEHNTRSIKQVLMDQTIIAGIGNIYSDEILFDAQISPLRKANSL
                     TGVEIEKIYKSIKKILKLAIRYKGTSVSDFRDINGYPGEFSKMLKVYNKKSGKCVKCH
                     SDIKTIKINGRTAHYCPKCQK"
     gene            1405..1761
                     /locus_tag="P5241_03115"
     CDS             1405..1761
                     /locus_tag="P5241_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32505.1"
                     /translation="MFDINELKNVLETQCLFNNKKVVLIKNLLTLLNQHIGKDNLNIH
                     NKVNEDIDVENLSAKNNKHKQLFQEIFDLIKHNDSKDVLIILWDNIMVLKKQYLKDVK
                     YKEEVFPVLKGKPLLK"
     gene            1927..2133
                     /locus_tag="P5241_03120"
     CDS             1927..2133
                     /locus_tag="P5241_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32506.1"
                     /translation="MPNNPINFELSNAIADKNRAKSLQALYKELDAYTKPEILVGSIA
                     SQIRRLLIVKTLETTPNLSFKSLN"
     gene            2457..2870
                     /locus_tag="P5241_03125"
     CDS             2457..2870
                     /locus_tag="P5241_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV32507.1"
                     /translation="MKHQKAATDSKRGKIFSKFAGSIALAAKDGGDPNTNTKLRLLVD
                     KAKSLGMPKDNIERAIKRGSGVGGEGAIFEEIIIEAYGPQSSGILIQAATDNKNRTLP
                     EIKKILQINGAKIAQSGSVKYMFKEIGNIVVKKNL"
     gene            2880..3026
                     /locus_tag="P5241_03130"
     CDS             2880..3026
                     /locus_tag="P5241_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32508.1"
                     /translation="MALMVIESGALDIIEDDDDIIIKTNSQDLNKIKMILEKSVDIDD
                     YYLD"
     gene            3224..3724
                     /gene="ruvC"
                     /locus_tag="P5241_03135"
                     /pseudo
     CDS             3224..3724
                     /gene="ruvC"
                     /locus_tag="P5241_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
     gene            3759..4139
                     /locus_tag="P5241_03140"
     CDS             3759..4139
                     /locus_tag="P5241_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32509.1"
                     /translation="MTNSYKSMLSKQDDEDKELESDSSKEESEENDEVASDDTEEEKQ
                     EKDKSEELAGAIDKKVITDNSSSDDSDSDEGAEEEAVLFDKEEDENIVLEDDIIINEV
                     QSIPFTEKEEKEFLDSYDHFLKED"
     gene            4149..4274
                     /locus_tag="P5241_03145"
     CDS             4149..4274
                     /locus_tag="P5241_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32510.1"
                     /translation="MNFKIPTIIIKIVQEIKQQGFECYLVGGCVRDLLLEAKPND"
     gene            4278..4952
                     /locus_tag="P5241_03150"
     CDS             4278..4952
                     /locus_tag="P5241_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HRV32511.1"
                     /translation="MTTNAKPEEIQKIFSQSIYENQFGTVGILTGVSENTLKIVEVTT
                     FRLEGRYSDKRHPDDIKFATTLQEDLSRRDFTINALALDIDLTHIKINTDKLVTPLSV
                     KCQLIDLFNGRDDLKKGIIRAVGDPDKRFDEDALRLIKTVRCATQLSFMIEQNTLESL
                     KRKAGNLQFIALERIRDELIKIIMTPNAADGVRLLQNLGLLDYVIPELIQTIGVAQNR
                     HHIYTV"
     gene            4959..>5452
                     /locus_tag="P5241_03155"
     CDS             4959..>5452
                     /locus_tag="P5241_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV32512.1"
                     /translation="MLKSLDYSAKENYSLEVRLAALLHDIGKPQTKQGEGVNATFLNH
                     EIASAKMAKHILNRLHFPNYILDKVVLLVRYHMFFYDPEISTDASLRRLLVKVGKDNI
                     EDLAKLREADRIGSGCPKALPFRLRHFMFRIEKILKDLQGETPSLKMLKVNGNDLMQN
                     IDIEP"
BASE COUNT         2192 a          586 c          957 g         1717 t
ORIGIN      
        1 aaaaagattt attaatttgt ttttttattt aatttttatg ccaaaagtct aaaatgttat
       61 aaacgtctac aaagtattgt atattttgtt aaaatatgtt agtatggaga tatataaaca
      121 attaaaatat tattatgcca attattaaat cagcaaaaaa agctttaaaa caatcaaaaa
      181 agagacatat tagtaattta aattataagc aacaaattcg tcatttgaaa aaggaaatag
      241 aaaaaagtat caaagcaggt aaaacagcag aagtagaaga attgatcaaa aaattttata
      301 aagccacgga taaagctgct aaagagcatg taattcataa aaacaaagca gccagattaa
      361 aatccaaagc agttaaaaaa attcagcgat ctcatagtta ataattttgg tattatgcca
      421 gagttgcctg aagtaactac tatagttaag ggtttacaac acaaaataat agggtttaga
      481 ataaaaagcg tttgaacaga ttacaagaaa aacgctaaaa atttgaattt attaaaaggc
      541 aaggtgataa aaaaggttac acgacgaggg aaaaatattt tgatttattt atcgcaagat
      601 tatatattat taatacatca aaaaatttct ggccatttat tattgggtag atgaatattg
      661 aaaaatggtg tatgagtacc tatgcaaaac aatggtataa gtttggatag ggttaataat
      721 tatattcatt ttatgataac ttttgataat ggcgagatgt tagctctgtc tgatttgaga
      781 aaattcgcca aaataatagt tggcaaaaag caagatattg agtcgctaaa agaattggtg
      841 agtttgggta aggatccttt tgataaaact tttacaaaaa cttgattaat aaaaaaaatt
      901 gaacacaata cacgcagtat aaaacaagta ttaatggatc aaacaattat cgctggtatt
      961 ggcaatattt attctgatga aattttattt gatgctcaaa ttagtccttt gcgtaaagcc
     1021 aatagtttga ctggtgtaga aatagaaaaa atatacaaaa gtattaaaaa aatattaaaa
     1081 ttagcaattc gttataaagg aacttctgtt tcggatttta gagatattaa tggatatcca
     1141 ggagaatttt ctaaaatgtt aaaagtatat aataaaaaat ctggtaaatg tgtaaagtgc
     1201 catagtgata tcaaaactat taaaattaat ggtagaactg ctcattattg tcctaagtgc
     1261 caaaaataac taaattatat tatggttata ttaatttatg gtggcgattt atttaattcg
     1321 tttagaaggg tcaaggaatt attggctgat tatcaatcta agggatgaga tatttttaga
     1381 tttgatgtat tagataaatc agaaatgttt gatattaatg aattaaaaaa tgttttagaa
     1441 actcagtgtt tgtttaataa taaaaaagtt gtgttaatca aaaatttatt aacattatta
     1501 aatcaacata ttggcaaaga taatctaaat atacataaca aagtaaacga agatattgat
     1561 gtagaaaatc taagtgccaa aaataataaa cataaacaat tatttcaaga aatattcgat
     1621 ctaataaaac acaatgattc taaagatgta ttgattatac tttgggacaa tataatggta
     1681 ttaaaaaaac aatatctcaa agatgttaaa tataaagaag aagttttccc ggtgttaaaa
     1741 ggaaagccat tgctcaaatg agcaactata gaagccacta aattaggaat aatagcagat
     1801 aaagaagttt tggaagtttt gactatggcc tgagatggag atacttgggc gatttataat
     1861 gaattagaaa aattatcgtt attaaaaacc aaaataacct taaaaaattt tttagatttt
     1921 tctgtattgc ccaataatcc aattaatttt gaattatcaa atgctattgc tgataaaaat
     1981 cgagcaaaat ctttgcaagc tttgtataaa gaattagatg cttatacaaa accagaaatt
     2041 cttgtaggat caattgcttc tcaaataaga cgattactta tagttaaaac actcgaaaca
     2101 accccgaatc taagttttaa gagtttaaat tgaaacccat ttgctttaca aaaaacgcgt
     2161 caactggcgc aaaaatttag tctagaaggt ttaaaaagag aatatagagt tttgtttcga
     2221 tatgatgttc aaattaaaaa agggaaaata gatcctgtat tggcattgga attatttatt
     2281 aatgatatta ccagatagat tatatgtgcc atattcggac aattagtatt taaatatgta
     2341 atattgaagc aacatttatt attaaggggt tgctttttta tggaaaatgt tgtaaaatac
     2401 aataataaca attgaataaa ttttttatta tgtcaggaca ttctaaatga gcttcaataa
     2461 agcatcaaaa agctgcaact gatagcaaaa gaggtaaaat tttttctaaa tttgctggta
     2521 gtattgcttt ggcagcaaaa gatgggggag atcccaatac aaatacaaaa ttacgtttat
     2581 tggtagataa ggcaaaaagt ttaggtatgc caaaagataa tattgaacga gccattaaaa
     2641 ggggtagtgg agtaggaggt gaaggagcaa tttttgaaga aattattata gaagcctatg
     2701 gaccccaaag ttctggtatt ttaattcaag cagcaactga taacaagaat agaacattgc
     2761 cagaaattaa aaaaatttta caaataaatg gggcaaaaat agcccaaagt ggcagtgtaa
     2821 aatatatgtt taaagaaatt ggtaatattg tggtcaaaaa gaatttatga aatgatgatt
     2881 tggctttgat ggttatagag tcaggtgctt tggatattat tgaagatgat gacgatatta
     2941 ttattaaaac gaactctcaa gatttaaata aaatcaagat gattttagaa aaatcagtag
     3001 acatagatga ttattatttg gattgagaaa gtgatcaaaa aatacagatt gaagacgctc
     3061 aagaaaaaga aaaaatagaa aaattattta acgaattgaa tgacaatgat gatgttatag
     3121 aagtatactc caatatatca ttttagtttg ggcttatttg aataagtatt tattataata
     3181 tgtttggttg tttggatatt tttaattata taattttgat ataatgattg tattaggtat
     3241 tgatcctggc acttcaagaa cgggttttgc tgttattaga caaacaaaat ccaaagtaaa
     3301 acctattgaa tttgggtgtt gagatataca aaataaaacc caatatcaaa agctgaatat
     3361 aatatttaac aatttgacaa aattgataac taaatatcat ccagatcttt ttgcagttga
     3421 acgattattt ttctttaaaa acgccaaaac agttatgaca gttagcgagg cacggggagt
     3481 attattattg gctgctaata agaaaaaatt ggatattgta gagatcactc cattggaagt
     3541 aaaacaatat ctattgggtt atggtagatc tgaaaaatta caagtgcaaa aatttgtagc
     3601 aatgtatttg gatctaaaaa caatacccaa acccgatgat gtcgctgatg ctttggcaat
     3661 ttgtttggca ggtataataa aaaaatcaaa acaaaataaa caatcaaaac aaaaaatcaa
     3721 gtaatttttt aacaaactta ataaaataaa aatatattat gacaaattca tataaatcta
     3781 tgttatctaa gcaagatgat gaagataaag aattagaatc agatagctcc aaagaggagt
     3841 cagaggaaaa tgatgaggtg gctagtgatg atacagaaga agaaaaacaa gaaaaagaca
     3901 aaagcgaaga attagcaggg gctatagata agaaagttat aacagataat agtagttctg
     3961 atgatagtga ttctgatgaa ggggcagagg aggaagctgt tttgtttgat aaggaagaag
     4021 acgaaaatat agtattagag gatgatataa ttataaacga agtccagagt atacctttta
     4081 ctgaaaaaga agaaaaagaa tttttggata gttatgatca ttttttgaaa gaagattaat
     4141 tttatattat gaattttaaa attccaacaa ttattattaa aattgttcaa gaaataaaac
     4201 aacaaggatt tgagtgttat ttggtagggg gatgtgtgcg tgatttattg ttggaagcca
     4261 agcctaatga ttgagatatt actacaaatg ctaagccaga agaaattcaa aaaatttttt
     4321 ctcaatcaat ttatgagaat caatttggca cagtgggaat attaacagga gtttcagaaa
     4381 atactttaaa aattgttgaa gttactactt ttagattaga aggtcgttat tcagacaaaa
     4441 ggcacccaga tgatattaaa tttgccacaa ctttacaaga agatttgtcg cgacgtgatt
     4501 ttactataaa tgctttggcg ctggatattg atttaacaca tataaaaatc aatacagaca
     4561 aattagtaac cccactatca gtaaagtgtc agttgattga tttgtttaat ggtcgggatg
     4621 atctaaaaaa gggaattatt cgagcagtag gcgatccaga taaaagattt gacgaagatg
     4681 ctttaaggtt aataaaaact gttaggtgtg ctacacagtt atcttttatg attgaacaaa
     4741 atactttaga gtcattaaaa aggaaggctg gtaatttaca atttattgct ttggaaagaa
     4801 ttcgtgatga actaatcaaa attattatga cgcccaatgc tgctgatgga gttcggttat
     4861 tacaaaatct cggattgctt gattatgtta ttcctgaact tattcagact ataggtgtag
     4921 cccaaaatag acatcatatt tatacggttt gagagcactt gttaaagtct ttggattatt
     4981 cagcaaaaga aaattatagt ttagaagtgc gattggcggc tttattgcat gatataggta
     5041 agcctcaaac taaacaggga gagggagtaa atgctacatt tttaaatcat gagatagcga
     5101 gtgccaaaat ggctaaacat attttaaata gattacattt cccaaattat attttagata
     5161 aagtagtttt gttggtaaga tatcatatgt ttttttatga tcccgaaata agcactgatg
     5221 cttctctaag aagactatta gtaaaagttg gcaaagataa tattgaagat ctagctaagt
     5281 tacgagaagc cgatagaatt ggctcagggt gtccaaaagc tttaccattt agattgagac
     5341 attttatgtt tcgaatagaa aaaattttga aagatttaca aggagaaaca ccaagtttaa
     5401 aaatgttaaa agtaaatggt aatgatttaa tgcaaaatat tgatatagaa cc
//
LOCUS       DAOTJA010000041         5311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13851_length_5311_cov_5.614155, whole genome shotgun sequence.
ACCESSION   DAOTJA010000041 DAOTJA010000000
VERSION     DAOTJA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13851_length_5311_cov_5.614155"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5241_03160"
     CDS             <1..205
                     /locus_tag="P5241_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRV32513.1"
                     /translation="PADMGHLIGRQGTTIQAIRTILRIIGARNQARINIKIPGGEGHP
                     SPRQDTASQHSSEVDKALDDLQL"
     gene            363..965
                     /locus_tag="P5241_03165"
     CDS             363..965
                     /locus_tag="P5241_03165"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV32514.1"
                     /translation="MLLLFGLLVIAEQLFLMIVNIKEISIQNNTHKQITESVESRLSQ
                     DAQDAQRQEYFSLVLPEEKAILINLSKMKLYLIQNKKIIQEFDVATKGPADKWFRTPA
                     GYYKIGAKYPLLRSSKVDVYMPYSLQFYEDFFIHGIPYFPNGVKVTSSFSGGCLRLQD
                     NDAKIVYDFADKGTMVVVIDEIPSLDSHSNFIYPVDIHKS"
     gene            996..1286
                     /locus_tag="P5241_03170"
     CDS             996..1286
                     /locus_tag="P5241_03170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV32515.1"
                     /translation="MGADYLQHTGVDFATIDLESVMAVFDGVVVYIQNIGPNDHGFGN
                     TIILEHNINQNKIYTLYGHLENIQEGLKEGDFVKKGDIIGMVGASGYGCSNY"
     gene            1320..1493
                     /locus_tag="P5241_03175"
     CDS             1320..1493
                     /locus_tag="P5241_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32516.1"
                     /translation="MIDRHLHFEVKTKPVLTNPDGDSLCHYNGNKGLCFGYTPKYPQD
                     YGYINPIDFLNQK"
     gene            1733..2209
                     /locus_tag="P5241_03180"
                     /pseudo
     CDS             1733..2209
                     /locus_tag="P5241_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            2519..2842
                     /locus_tag="P5241_03185"
     CDS             2519..2842
                     /locus_tag="P5241_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRV32517.1"
                     /translation="MAKKLYVGSLSYDTTEDTLKDLFSQYGEVTSVMIIVDKMSGRSK
                     GFGFVEMSSDEDAQKAIDALNGSDFEGRKIIVNEARPREERSRRNFNRGSNRNYGNFG
                     GNRGN"
     gene            3020..4096
                     /gene="ychF"
                     /locus_tag="P5241_03190"
                     /pseudo
     CDS             3020..4096
                     /gene="ychF"
                     /locus_tag="P5241_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
     gene            4235..5062
                     /locus_tag="P5241_03195"
     CDS             4235..5062
                     /locus_tag="P5241_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRV32518.1"
                     /translation="MLFDSHTHLQFDDFDKDRVDVIKRLIDNNIFIINVGSNIENSKM
                     ALDLSKQYSFMWVSVGVHPSEVITYSHSKTRVLNMLSQNMLLALRELAQNPKVVAIGE
                     CGLDLSYFKEIPDYELYQNEYNNYQYTMFRQQIRLAQELGKPIILHIRKLYEEAIKIL
                     EEEKFKNNVVFHFFKGKQKTLEQILEHSNYYLGFSGVITYDNSLDNIIFNTPLNRLLI
                     ETDAPYVPPIPKKGERNEPLFIKYTAEHIAKVKNLSLKEIEKITYENTLKVFNIQNV"
BASE COUNT         1976 a          578 c          929 g         1828 t
ORIGIN      
        1 ccctgctgat atgggacatt tgattggaag acagggcaca actattcaag ctataagaac
       61 cattttaaga attattggtg cccgtaatca agccaggata aatataaaaa ttcccggagg
      121 agagggtcat ccatctccaa gacaagatac tgcttctcaa catagttcag aagtagacaa
      181 agctctcgat gatttacaac tttagagagt agcacgattc ttaatttgat aacaaagagg
      241 agcgattttc tcgttcctct tttatttttt ttatttttat gataatataa ttttggatat
      301 catattatta atatataaca atatacataa tgaaaaaatc tgatattaag ttattgtttt
      361 gaatgttatt attatttggg ttgttagtta tagccgaaca attgtttttg atgattgtaa
      421 atatcaaaga aattagtatt caaaataata ctcataaaca aataacggaa tctgtagaaa
      481 gcagattgag tcaagatgcg caagatgctc agcgacaaga atatttttct ttggttttgc
      541 cagaagaaaa agctattttg ataaatttgt caaagatgaa gttgtattta atacaaaata
      601 aaaagattat tcaagaattt gatgtggcaa ctaaagggcc ggctgataaa tggtttcgta
      661 cgcctgctgg atattataaa ataggagcaa aatatccatt attgcgttca tcaaaagttg
      721 atgtatatat gccttattca ttgcagtttt atgaagattt ttttattcat ggtattcctt
      781 atttcccaaa tggtgtaaaa gttactagtt cgtttagtgg ggggtgtttg agattacaag
      841 ataatgatgc taaaatcgtt tatgattttg ctgataaggg cacaatggtt gtagtgatag
      901 atgaaattcc gagtttggat agtcattcta actttatata tcctgtggat attcataaaa
      961 gttgaataag acaaagtttt attagtcctc aaaaaatagg ggctgattat ttacaacata
     1021 caggcgtaga ttttgctaca atagatttag aatcagttat ggcagtattt gacggggttg
     1081 ttgtttatat tcaaaatatt ggaccaaacg atcatggctt tggtaataca ataattttgg
     1141 aacataatat taatcaaaac aagatatata ctttatatgg acatttagaa aatatacaag
     1201 aggggttaaa agaaggagat tttgtcaaaa aaggtgatat tataggtatg gttggagcca
     1261 gtggttatgg ttgtagtaat tattgacgta taggtcaaga tggttgtttt gaaccaaatg
     1321 tgatagatag acatttacat tttgaagtaa aaacaaaacc agttttgact aatcctgatg
     1381 gtgactcttt gtgtcattac aatggtaata aagggttatg ctttggttat actcctaaat
     1441 atccacagga ttatggttat ataaatccca tagattttct taatcaaaaa taacaatatt
     1501 tttaattatt gtttatttaa ttatttttta ttttttctgt ttttaatgaa taatatctaa
     1561 atataaaaat tttatggtaa aaaaacaaaa aatacaattt gatataatta ctatatatcc
     1621 tgagtgcttt cagtcttatt tatcttgtgc tatttttaac aaagcgataa aactcggtat
     1681 agttaaaata aaaattcata atttaagaga ttgaacttct gataaacata gaagtgttga
     1741 tgatagcccc tatggtggtg gtccgggtat gttaatgaaa atcaatccaa tatttgaatg
     1801 tttaaaggat attgtaggac agccattaaa aagtaaaaga acaaataaaa aaataaaagt
     1861 tattgtgttt actcccaaag gaaaagttta tactcaaaag atggcagcca aattttctaa
     1921 attagataga attattatga tttgtggtag atacgaaggt atagatgaaa gagttatcaa
     1981 atatttagcc gatatgaatg tttcgattgg aaattatgta ttgtcagggg gagaaattcc
     2041 cgcgttggta ataatggaat ctgtgacacg tctattgccg ggagttttag gtgcaagcga
     2101 atctttgaaa gaggaaagtt ttgtgccgtg tagtgattat ttagaatatc cacaatatac
     2161 cagaccatca attttttatc caaataaaaa aaataaaaaa atatcttgaa aagtcccaaa
     2221 agtattgtta tcaggagatc ataaaaaaat aaacacattt cgtcaccaag atattaagtt
     2281 atctaaacat acttttggtc aaaaaacaaa acagcttaac actaaataac cattgtttgt
     2341 attatttggg agtatactta gatattatta tattaaagct aaaatttagt aggggcttgc
     2401 aaaaaaatat aaaatgtagt ataataaaag tattgtaaga tttttcttaa gtaggagtta
     2461 taaacataat acatagaaaa ataagtcggc ttgcaaataa ttatttagaa tttatagtat
     2521 ggctaaaaaa ttatacgttg gcagtttatc atatgatacc acagaagata cattaaaaga
     2581 tttattttcc caatatggtg aagttacttc tgttatgatt attgtagaca aaatgtctgg
     2641 tcgttctaaa ggatttggtt ttgtagagat gtcttcagat gaagatgctc aaaaagccat
     2701 tgatgctttg aatggttctg actttgaagg acgcaaaatt attgtaaatg aagctcgtcc
     2761 aagagaggaa cgttcaagaa ggaattttaa tcgtggaagc aacagaaact atggcaattt
     2821 tggaggtaat cgtggaaatt gataaatgct atttagataa cacaaacgat aaaaagggag
     2881 agtaatctct ccctttttta gtaatttttt gtcaatgata ttttaagtaa tccttgataa
     2941 aaaacagtaa ttgtgctaaa atgaatttaa caatcagtat tgtttttaat tagctacatt
     3001 atataaaata gaataaataa tgaaactatc tataggtata gttggtttgc ctaatgttgg
     3061 taaatctact ttatttcaaa caattaccaa aaaacaagta gatattcaaa attatccctt
     3121 ttgcactatt gagccaaatg tgggtgtagt ttttgtacct gatgagcgat tagaaaaatt
     3181 agcattgttt agtcattctg ctaaaattat accagcagta gttgaatttg ttgatattgc
     3241 tggtttggtt aaaggagcta atcgaggtga ggggttgggt aataaatttt tatcgcatat
     3301 tagagaagta gatgctatta tttatgtatt gcgtgctttt aaaaatccta atattataaa
     3361 tgtttctaac aatattgatc caatcgcaga aaaagagata ttagatacag aattgatgtt
     3421 aaaagattta gagacagtga ataaaaggat tggttctttg gaaaaagaaa aaaagagtag
     3481 actaaaagaa gctatgatag aatatgatat ttttatgaaa ttaaaattag gtttagagga
     3541 tggtaaacaa ataagagatt tacaattaga tacagaggaa caaaatatca ttaaacaata
     3601 tcaatttttg actaataaat cttgtttgta tgtattgaat ggtaaagacg aagaagtaga
     3661 tacgagtata attgattatt ttgtgaataa taagcttgat tatttaatta ttgatataaa
     3721 aacagaattt gatatagtag atttaaacga agaagaaaga aatttaatgg gtattgcatc
     3781 caaacctgaa ttagatttgt tgattcaaaa agcttataca attttaaatt tgatttcttt
     3841 ttttactact ggaaaagatg aaacgcgtgc ttggactatt acccgaggta ccaaagctcc
     3901 tcaggcagca ggagtgatac acggcgattt tgagaaatat tttattaggg cagatgttat
     3961 tagttgagat aaacttttgg aggctttgtc gtgagaaagg gcaaaatctt tgggtatagt
     4021 taggagtgaa ggcaaagaat atattgtaca ggatggtgat gttttagaaa tcaaacatgg
     4081 attgcccaca gattaacaca tatagcaaga atatttggat aaacattgat aagcttatta
     4141 gtttatgagt ataaaagtaa ataagtttaa aagtttatta gaaatttgta gaaaaatttt
     4201 tagcagggat aaaaataaaa attaaaaaac aaaaatgttg tttgactctc atactcattt
     4261 acaatttgat gattttgata aagatcgagt tgatgttata aaaagattga ttgataataa
     4321 tatttttatt atcaatgttg gttctaatat tgaaaattca aaaatggcgt tagatttatc
     4381 aaagcagtat tcttttatgt gggtttctgt tggtgtacat ccctctgagg taataacata
     4441 ttcacattcg aaaactcgag ttttgaatat gttatcccag aatatgttat tggcattaag
     4501 agaattggca caaaatccaa aagtagtagc gatcggagaa tgtggtcttg atttgtctta
     4561 ttttaaagag attcctgatt atgaattata tcaaaatgag tataacaatt atcagtatac
     4621 aatgtttcga caacaaattc ggctagccca agaattgggt aaaccaatta ttttacatat
     4681 tagaaagtta tatgaagaag ctattaaaat tttagaagag gaaaaattta aaaacaatgt
     4741 tgtttttcac ttttttaaag gtaaacaaaa aactttagaa caaattttag aacattcgaa
     4801 ttattatctc ggtttttccg gagtaattac ttatgataat agtttagata atattatttt
     4861 taatacacct cttaatagat tacttattga aacagacgct ccttatgtac cacctattcc
     4921 caaaaaaggt gagagaaatg agcctttgtt tataaaatat actgctgaac acatagccaa
     4981 agttaaaaat ttatcattaa aagaaataga gaaaattact tatgaaaata ctttaaaagt
     5041 ttttaatatt caaaatgtat aaaaaaataa aaataacaaa tattattata tatttagttg
     5101 tattgctgtt ttgcgttgtg gttatttttt tgttaggctg aggaaaaaaa caacaactta
     5161 tgcaatctaa aaaaaatatt gagactatat atgttggtgg tattgtaaat catcatttgt
     5221 tagctcgtga tattatagaa aaatttttta tggtatcttc caaatttaat tatgataaaa
     5281 ttgttatttt aagtccagat cattttaata g
//
LOCUS       DAOTJA010000042         5296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13900_length_5296_cov_5.609807, whole genome shotgun sequence.
ACCESSION   DAOTJA010000042 DAOTJA010000000
VERSION     DAOTJA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13900_length_5296_cov_5.609807"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1781)
                     /locus_tag="P5241_03200"
     CDS             complement(60..1781)
                     /locus_tag="P5241_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRV32519.1"
                     /translation="MGIDLTDLASKMKLGFLVGHEQEFDSLMNVLARPGRNNVLLVGE
                     PGTGKETMVANLAIKIIKDEVPGNLFDKRLVALNIGNVTSGANNVGELQNRFQKLAKE
                     MLEAGNIVLYIPNIYTLKQTNVEGAGINAADLLKPIFQSGLVPVIGTATEQEYRRLLD
                     TDVEFAGMFEIIRLQEISEDEAIQILSYEALSLEKQYRIVISYKAIKRAVFLSKRYLR
                     DKLLPASATDLLRETVVYIKSIGKKIVSEDDIIDMVEAKTRIPMANVTKQEAEQLLNL
                     EDKLHQRMIDQEEAIKSVAEALREYRAGLSNKSGPIASFLFVGPTGVGKTELAKSLAV
                     IYFGSESKMIRFDMTEYQDVESVQRFIGSPDGEKLGALTEAVRIHPFSLILLDEFEKA
                     SQNIMNLFLQVFDDGRLTDSTNRVIDFSNTIIIATSNALSSFIKEELDKGTEYKQLSE
                     QIKLKLTSVYTPEMLNRFSKILVFKPLSPDDILEITKLQIKNLAEQIFNDKEIKLSVS
                     DSAIAELARLGFDPVFGARPLQGVIREKIKSEIANKILKQELVSGDEIIIDFQNNEFT
                     YTKIKIF"
     gene            complement(1812..2075)
                     /locus_tag="P5241_03205"
     CDS             complement(1812..2075)
                     /locus_tag="P5241_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32520.1"
                     /translation="MSLAFIEAIGLRENYISKRALFLALFQDDYSNIFKLKCKFQIDK
                     KDLYNALILSSIPVPKPEELSIGVGDFVRLRMKYGYRFKVNRS"
     gene            complement(2132..2491)
                     /locus_tag="P5241_03210"
     CDS             complement(2132..2491)
                     /locus_tag="P5241_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32521.1"
                     /translation="MRIGFFVFLMISGICGLVLLNSDLKGIGIIILIIIINYIITISR
                     PEETITLDTFNKDIIKIDSYISEQNKNLVLNTFLDCLVKKTDFYLEIFYKLIQDPNIS
                     KSLQKIDIDVVKLREIY"
     gene            complement(2930..3691)
                     /locus_tag="P5241_03215"
     CDS             complement(2930..3691)
                     /locus_tag="P5241_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRV32522.1"
                     /translation="MSGFDTIEDCFEELTPYVKICETGLSSDPLMNWRLSQLDNITLV
                     SNSDSHSLEKLGREANVFDIDDVSYNALYNVLTNKDARRFLYTIEFYPEEGKYHYNGH
                     RLCKVNLSPTESKKINNICPVCHKKLTIGVLHRVEDLADRSEDEDRQFGVIPFKSLIP
                     LKEIIADYYEVKSASKKVDTLYDLMLHQYGTEFNILLDISLGQMQQDGFKDIGQYIDK
                     VRKGEVEKIPGYDGEYGVIHIKKQTSSNVQNLFTT"
     gene            complement(3719..4177)
                     /locus_tag="P5241_03220"
     CDS             complement(3719..4177)
                     /locus_tag="P5241_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32523.1"
                     /translation="MRVIADFHIHTKYARATSQDMDLEHIADFADKKGINIIGTGDFT
                     HPMWFKELSNKLEEAESGLYKLKNSNTKVRFVLSSEISNIYTKNNRCRKIHSVILMPN
                     LQSVEEFNRRLSFRGNLNADGRPIIGMDAKDLLELSLEVDKQSIFIPAHI"
     gene            4240..4542
                     /locus_tag="P5241_03225"
     CDS             4240..4542
                     /locus_tag="P5241_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRV32524.1"
                     /translation="MSLELGKLGESLTQKYLKSKKYKILDTNFRLNYGEIDIVAKYHK
                     IIIFIEVKSRQFNANVAQNTNPPILPEEEFTITKQKRLLRAINTYLKIHKYPEYVD"
     gene            complement(4860..4930)
                     /locus_tag="P5241_03230"
     tRNA            complement(4860..4930)
                     /locus_tag="P5241_03230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4896..4898),aa:Pro,seq:cgg)
     gene            complement(5028..>5296)
                     /locus_tag="P5241_03235"
     CDS             complement(5028..>5296)
                     /locus_tag="P5241_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32525.1"
                     /translation="KMLKVNGNDLMQNIDIEPGPKLGFILNILLEEVLDDISKNTKEY
                     LLQKAKELNNKDIQELKELSEKARDRYNQILNEEEAEIKRKYYV"
BASE COUNT         1883 a          914 c          560 g         1939 t
ORIGIN      
        1 ataaatatat atacataata cttttttgaa tgattagaat aattaaacat agtatgagat
       61 taaaatattt taattttagt atatgtaaat tcgttatttt gaaaatctat tattatttca
      121 tcgccactaa ctaattcttg tttcaaaatt ttattagcta tttcggattt tattttttca
      181 cgaattactc cctgcaaagg tctagctcca aatactggat caaaaccaag tctagccaat
      241 tctgcaatcg cactatcaga tacactaagc ttgatctctt tgtcattaaa tatttgttca
      301 gccaaatttt taatttgtaa ttttgtaatt tccaaaatgt cgtctggtga caaaggttta
      361 aaaactaaaa ttttgctaaa tcgatttaac atttcagggg tatatacaga agttaatttc
      421 aatttaattt gctctgataa ttgtttatat tcagtgcctt tatctaattc ttcttttata
      481 aaagatgata aagcattgga agtagcaata ataatagtat tgctaaaatc aatcactcta
      541 tttgtactat cggttagtct accatcgtca aaaacttgca aaaataaatt cataatattt
      601 tgtgaagctt tttcaaattc atcaagtaaa attaaagaaa aagggtgtat tcttacagct
      661 tcggtcaatg cgcctaattt ttcgccatct ggacttccaa taaatctttg aacgctttca
      721 acatcttgat attctgtcat atcaaaacgg atcattttag attcgctacc aaaatagata
      781 acagcaagac tttttgctaa ttctgttttt cctacacctg ttggaccaac aaacaaaaac
      841 gaagcaatgg gtcctgattt attcgacaaa cctgctctat attctcgtaa cgcttcagca
      901 actgatttta tagcttcttc ttgatcaatc attctttgat gtaatttgtc ttctaaattt
      961 aataattgtt ctgcttcttg ttttgtaaca ttggccatag gaattcgagt tttcgcctcc
     1021 accatatcaa ttatatcatc ttcggacact atctttttac caatagattt tatatataca
     1081 actgtttctc ttaataaatc agtagcagaa gcaggtaaca atttatcacg taaatatctt
     1141 ttagacaaaa ataccgctct tttaatagct ttataagaga taactattct atattgtttt
     1201 tccaaactta aagcctcgta gctcaaaatt tgtattgcct catcttctga tatttcttgc
     1261 aatcgaataa tttcaaacat acctgcaaat tccacatctg tatctagtag tcttctatat
     1321 tcctgctcag tagccgtgcc aattacagga acaagcccag attgaaatat tggttttaac
     1381 aaatctgcag cattaatccc tgcaccttct acattggttt gtttcaaagt ataaatattc
     1441 ggaatatata aaacaatatt ccctgcttct aacatttctt tggcaagttt ctgaaatcta
     1501 ttttgtaatt cacctacatt attagcacca ctagttacat tgccaatatt tagagcaaca
     1561 agtcttttgt caaataaatt tcctggcact tcatctttta taattttaat agccaaattc
     1621 gccaccattg tttctttgcc tgtgccaggc tcccctacca ataatacatt gttgcgtccg
     1681 gggcgtgcta aaacattcat caaagaatca aattcttgtt cgtgacctac caaaaatccc
     1741 aatttcattt tagaagccag atctgttaaa tcaataccca cttcgtctaa ataatctgtt
     1801 ggcctagcag ttcatgatct atttacttta aacctataac catatttcat tctcaatctt
     1861 acaaagtctc ctacacctat tgacaattct tctggtttgg gtacgggtat actagataaa
     1921 atcaaagcat tatataaatc ttttttatct atctgaaatt tacatttaag tttaaaaata
     1981 ttactataat catcttgaaa taatgccaaa aataatgccc gttttgatat ataattttct
     2041 cgcaagccaa tggcttcaat aaacgctaaa ctcatataat tactgatatc ttcttttcgt
     2101 atcaaagtat ttattggtat ttttaattga tctaatatat ctctcttaat tttacaacat
     2161 caatatcaat tttctgtaaa cttttggata tattaggatc ttgaattagt ttataaaata
     2221 tctctaaata aaaatcagtt tttttaacta aacaatccaa aaaagtattc agaactaaat
     2281 ttttattttg ttcgcttata taagaatcga tctttataat atctttgtta aatgtatcaa
     2341 gagtaatagt ttcttcgggc cgagatattg ttataatata atttataata ataatcaaaa
     2401 ttattatacc aatacctttt aaatctgaat ttaataagac taacccacaa atccccgaaa
     2461 tcattaagaa cacaaaaaat ccaatacgca aaataatatt ttgtcatagt ggaaaattta
     2521 atctctcgtc agtataaact atctttttat tctttaaatt tattaaactc atattactat
     2581 ttaaaagtat taaaaataaa taagggcaac aaacaccata acccataaac agccaaaaag
     2641 acaacaaaaa tgataaaata agtaatactt gataaaatta ctctaaataa tctaaatccc
     2701 acacccatta tataaccact ccaagtataa tcttgatata aaggctgaaa aaaatgttgt
     2761 aaagttattt ttaaagctca cttgcgatcc aattttttaa ttagtttgat aaacatatta
     2821 tatacaaaat aaaagctatc aatataccaa tgctttaaag tagatataat catccaccca
     2881 atttttttaa aaaaataaag taaaaacata tatgatattt taaatatatt tatgttgtaa
     2941 ataaattttg aacatttgat gatgtttgct ttttaatatg tataactcca tattctccat
     3001 catacccggg tatcttttct acttctcctt ttcgaacttt atcaatatat tgcccaatat
     3061 ctttaaaccc atcttgttgc atttgcccca aagaaatatc aagtaaaata ttaaactctg
     3121 tgccatattg gtgtagcatt aaatcatata aagtatccac tttttttgaa gctgatttta
     3181 cttcataata atcagcaata atttctttta aaggtatcaa agatttaaaa ggaataacac
     3241 caaattgtct atcttcatct tcactcctat cagccaaatc ttctactcta tgtaatactc
     3301 ctattgttaa ttttttatga cacactgggc aaatattatt tattttttta gattctgttg
     3361 gcgataaatt tactttacat aatctatgcc cgttataatg atatttacct tcctctggat
     3421 aaaattcaat agtatacaaa aatctacgag catctttatt agttaaaaca ttatataacg
     3481 cattgtaaga cacatcgtca atatcaaata cattggcttc cctgcccaat ttctccaaag
     3541 aatgactatc tgaattggat accaatgtga tattatccaa ttgtgataat ctccaattca
     3601 ttaaaggatc tgaggataaa cccgtttcac agattttaac ataaggagtc aactcctcaa
     3661 aacaatcttc tattgtatca aatcctgaca tagagccaaa caaagaaaat cagggagttc
     3721 aaatatgtgc tggaataaaa attgattgtt tatctacttc taaacttaat tctaaaagat
     3781 ctttggcatc catgccaata atcggcctac catcagcgtt tagattaccc cgaaaagata
     3841 atcttctatt aaattcttcg acactttgca aattcggcat caaaataaca ctatgaattt
     3901 tcctacatct attattttta gtataaatat tacttatttc cgaagacaaa acaaatctta
     3961 cttttgtatt gctgtttttt aatttatata aacctgactc tgcctcttct aatttattag
     4021 ataactcttt gaaccacatt ggatgagtaa aatctccagt accaataata ttaataccct
     4081 ttttatcagc aaaatcagca atatgttcca aatccatatc ctgactagta gctctagcat
     4141 attttgtatg aatatgaaaa tctgcgataa ctctcattat agtaaattaa gcttttttat
     4201 gctataataa ttatatcaca atatttaaaa ttttgctata tgtctttaga attgggaaaa
     4261 ttaggagaga gccttacgca aaaatattta aaatctaaaa aatacaaaat tttagacaca
     4321 aattttagat taaactatgg tgagattgat attgttgcta aatatcacaa aataattata
     4381 tttatagaag taaaatctcg acaatttaat gctaatgttg cccaaaatac aaatcctcct
     4441 atactaccag aagaagaatt cactattacc aaacaaaaac gacttctacg agctattaat
     4501 acttatttaa aaatacataa atatccagaa tatgtagatt gacgattaga tttaatagct
     4561 gtttattttt atcccgataa ttatgaaata atccattatg aaaatgccct taatatccaa
     4621 taaatggtta cttactaaca aaaaaactaa aatttttaaa ataaaacaca atttaaaaat
     4681 aaaacacaaa ttcattatca tacaaaacac actaaacatt aactactcaa actaactaaa
     4741 aaaataatca tataaataaa aaatcccagc aaagtaattt atcagatatt tttacttttg
     4801 gaattataat ttacctatta tcattaaatt gctaatagac tcaactatgt ttagatttgt
     4861 cggggcattc ggatttgaac cgaaactact gactcccgaa gccagtggac taccattatc
     4921 ccataccccg ctcttggctt ctttttaaaa actcatattt attcaaaatc aaaaaaagaa
     4981 aaaatattat attctaaaac tttatctgta acttattact tacacattta tacataatat
     5041 tttcttttta tttcagcttc ttcttcgttc aatatttgat tatatctatc tcgggctttt
     5101 tcagacaatt cttttagttc ttgtatatct ttgttattca attctttggc tttttgtaat
     5161 aaatattcct ttgtattttt agaaatatca tccaaaactt cttctaataa aatattcaaa
     5221 ataaatccta attttggtcc tggttctata tcaatatttt gcattaaatc attaccattt
     5281 acttttaaca ttttta
//
LOCUS       DAOTJA010000043         5273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_13965_length_5273_cov_6.235339, whole genome shotgun sequence.
ACCESSION   DAOTJA010000043 DAOTJA010000000
VERSION     DAOTJA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13965_length_5273_cov_6.235339"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="P5241_03240"
     CDS             complement(<1..245)
                     /locus_tag="P5241_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRV32526.1"
                     /translation="MLSKKFQKRIEDFRCIHCHTLVKGNGYTDHCPKCLYSVHKDIYP
                     GDRLSDCDGIMKPVNIETVGGINYICYICEKCGYKHRV"
     gene            complement(249..557)
                     /locus_tag="P5241_03245"
     CDS             complement(249..557)
                     /locus_tag="P5241_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32527.1"
                     /translation="MMAIIDAQGGNPTIDSEQVIVGEYKYEVIAENTGLIQFINNQNL
                     VEICRLLGAPELKGSGIYLNKNVGEKYQKGDVLFTLYSESEQRLNSARGILDQLSIFK
                     "
     gene            complement(564..1013)
                     /locus_tag="P5241_03250"
     CDS             complement(564..1013)
                     /locus_tag="P5241_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32528.1"
                     /translation="MAPADDYFIKITHSLGIEPYSKMVVSVMAKKVAMGITHLIIDIP
                     VARTAKIPDMDTALKIKDIFLKIAKEFNIKIEVVITESNGPIGRGIGPALEMRDVLRV
                     LQQKDNRPMDLENKAIKLTGILLELTNRIAKGQGENRARENLISGVA"
     gene            complement(1029..1643)
                     /locus_tag="P5241_03255"
     CDS             complement(1029..1643)
                     /locus_tag="P5241_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32529.1"
                     /translation="MPGDELVLSWKNKETEVGVDISSEIVKPQEVGIFKDISSKYKIK
                     EGDLIKFTFANRAPSLQAIYKKLFRQKLTYKEILSIIDDIVKHRLNDIEIAFFIASAF
                     DEDNFSEEELYYLTKAIAQTGDTMKFEKITADKHSTGGLPGNRITPIIVPIVASFGVC
                     IPKTSSRSITSAAGTADAVETIMNVKFNTQQIYNLVKKNKGCLV"
     gene            complement(1686..1817)
                     /locus_tag="P5241_03260"
     CDS             complement(1686..1817)
                     /locus_tag="P5241_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32530.1"
                     /translation="MPNHDNNIEESYPLFAPALAERKKIDNNFFLKAKLVDISTGKN"
     gene            complement(1820..2716)
                     /gene="prs"
                     /locus_tag="P5241_03265"
     CDS             complement(1820..2716)
                     /gene="prs"
                     /locus_tag="P5241_03265"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRV32531.1"
                     /translation="MGNIVVGNKLAKHLSAKLKVKFCPVEFTKFIDGPVKPRILNFSK
                     AKFGILLLDMYQDIDINDYLINFLLLSAKMKEKCDKVIGIIPYLPYNRQDKEFLPGEP
                     VSAKIIGHYLSINLDYFITCNNHNHRLMLQDLFTIPSFDVSLFGELGKEFYDFDINNT
                     LIIGPDRESKPFVLDFIKNKPFDYIICNKSRNLNTNKVKITLPKYVKPNMLVNKDIII
                     VDDVIATGGTLLKLIPELKKYNIKSINFAFVHGLLINNTEQLLKSTKAKNIISTNTIF
                     NMFAKVDCVNAIFKVVNKILNK"
     gene            complement(2726..2956)
                     /locus_tag="P5241_03270"
     CDS             complement(2726..2956)
                     /locus_tag="P5241_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRV32532.1"
                     /translation="MIILYTAPGCPWCHVAKNFLDEHHIEYIEKDVSNPNYATELIQK
                     TGQTSIPVLDIDGQIVRGFDKEKIQELLNIQS"
     gene            complement(3323..3466)
                     /locus_tag="P5241_03275"
     CDS             complement(3323..3466)
                     /locus_tag="P5241_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32533.1"
                     /translation="MDSEINKYDVSLRYDLFANINSPSLLASEAAYCANEQGKFWEAN
                     EML"
     gene            complement(3503..3673)
                     /locus_tag="P5241_03280"
     CDS             complement(3503..3673)
                     /locus_tag="P5241_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32534.1"
                     /translation="MLLMVIVGYSVFFNNSLKKNTANTEIESSQIAKSLEPIGKTNAK
                     LDIVILADYECF"
     gene            complement(3826..4182)
                     /locus_tag="P5241_03285"
     CDS             complement(3826..4182)
                     /locus_tag="P5241_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV32535.1"
                     /translation="MVENDTDLITIEKIGKYKGEYHILGGLLSNIDEAKENSLTIKQL
                     IFKVRESIQNKKPIEEIILALNPTAEGYLTSLYLKKILEPYNIKITQIGVGIPYGGEI
                     EFADPETIKEAIKRRH"
     gene            complement(4303..4449)
                     /locus_tag="P5241_03290"
     CDS             complement(4303..4449)
                     /locus_tag="P5241_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32536.1"
                     /translation="MDIIPDDIHKLTGFFQKLPGVGSRQASRLSFFVAHYSQKNLKDL
                     AKLI"
     gene            complement(4483..4827)
                     /locus_tag="P5241_03295"
     CDS             complement(4483..4827)
                     /locus_tag="P5241_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRV32537.1"
                     /translation="MEDVYDVTVQDTHNFIANDIIVHNSIEQDSDVVMLIYREDKYKK
                     DSPNPNIAEILISKHRNGPVGQVQLYFNPEITSFRELDKNMTEEIEEGYTPLIPPDNT
                     ENNDFGEGFEEN"
     gene            complement(5065..>5273)
                     /locus_tag="P5241_03300"
     CDS             complement(5065..>5273)
                     /locus_tag="P5241_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRV32538.1"
                     /translation="PILVVSQLSRNVEQRTPPIPKLSDLRETGCLAFDSLIMRADTDE
                     VVKIKDLADRQEQVEIPVYTLDDN"
BASE COUNT         1752 a          904 c          614 g         2003 t
ORIGIN      
        1 actctgtgtt tatatccaca tttctcacaa atataacaaa tataatttat acctccaaca
       61 gtttctatgt ttactggttt cattatacca tcacaatctg acaatctatc tcctggatat
      121 atatctttat gcacagaata aagacatttt gggcaatgat ctgtataacc atttccttta
      181 actaatgtat ggcaatggat acaacgaaaa tcttctattc ttttttgaaa tttttttgaa
      241 agcataattt acttaaatat cgataattga tccaatatcc ctcgcgctga attcaatcgc
      301 tgttcgctct cgctatacaa agtaaataaa acatctcctt tttgatattt ttctccaaca
      361 tttttattta aatatatacc actacctttt aactcaggcg cacccaaaag tcgacaaatt
      421 tctactaaat tttgattatt aataaattgt attaacccag tattttcagc aataacttca
      481 tatttatatt cgccaactat tacctgctct gaatcaatag ttggattacc tccctgagca
      541 tcaatgatag ccatcatttt ttttcaagca actcctgata tcaaattttc acgcgctcta
      601 ttttcccctt gccctttagc tattctattt gtcagttcta ataaaatgcc agtaagttta
      661 atggctttat tttctaaatc cattggccta ttgtcttttt gttgtaaaac acgcaataca
      721 tcacgcattt ctaaagccgg gccaatacct ctgccaattg gaccattgct ctcagtaatt
      781 actacttcta ttttgatatt aaattctttg gctattttca aaaaaatatc tttaattttc
      841 aaagcagtat ccatatcagg aattttagca gttctagcaa ctggtatatc aataatcaaa
      901 tgagttatac ccattgctac tttttttgcc attacactca ctaccatttt agaatatggc
      961 tcaataccca acgagtgagt aatcttaata aaataatcat cagcgggtgc caaatttaat
     1021 gctccgcctc ataccaaaca ccctttattt tttttcacta aattataaat ctgttgagta
     1081 ttaaatttta cattcataat agtttccact gcgtcagctg tcccagcagc agaagtaata
     1141 gatcgagatg aagttttggg tatacaaaca ccaaaactcg caactatagg cacaataatt
     1201 ggagtaatcc tattgccagg taatccacct gtagaatgtt tatcagctgt aattttttca
     1261 aatttcatcg tatcgccagt ttgagcaatg gctttggtta aataatatag ctcttcctct
     1321 gaaaaattat cttcatcaaa tgccgaagca ataaaaaatg caatctcaat atcattaagt
     1381 ctgtgtttta caatatcatc tataattgat aaaatttctt tgtatgtaag tttttgtcta
     1441 aataattttt tataaattgc ttgcaatgat ggcgcacgat tagcaaaagt aaattttatt
     1501 aaatcccctt cttttatttt atatttgcta gatatatcct taaaaatacc tacctcttgt
     1561 ggtttaacta tttcacttga tatatctaca ccaacttctg tttctttatt tttccaagat
     1621 aataccaatt catcacctgg caaaatccca attcttttgg catcttcata atttataaca
     1681 acaattcaat ttttaccagt agaaatatca actaatttag ctttaagaaa aaaattatta
     1741 tcaattttct ttctttctgc caaagccggc gcaaacaaag gataagattc ttctatatta
     1801 ttatcgtgat tgggcatatt tatttattca aaattttatt aacaacttta aatatagcat
     1861 ttacacaatc aactttggca aacatattaa atatagtatt agtagaaatt atgtttttgg
     1921 ctttagtaga ctttaataat tgttctgtat tatttattaa caatccatga acaaaagcaa
     1981 aattaatact tttgatatta tattttttta gttctggaat caattttaat aatgttccac
     2041 cagttgctat aacatcatct acgataatga tatctttgtt aacaagcata tttggcttaa
     2101 catattttgg caaagtaatt ttgactttat tggtattcaa atttctcgat ttattacaaa
     2161 taatataatc aaaaggctta ttttttataa aatccaaaac aaagggcttg gattctcggt
     2221 ctggtcctat aattaatgta ttgttaatat caaaatcata aaattcttta ccaagctcgc
     2281 caaacaaaga tacatcaaaa ctaggaatag taaataaatc ttgcaacatt aacctgtggt
     2341 tatgattatt acaagtaata aaataatcta aattaatact caaataatgc ccaataattt
     2401 ttgccgaaac tggttctcca ggcaaaaatt ctttatcctg tctattataa ggtagatatg
     2461 ggataatgcc tataacttta tcgcactttt ctttcatttt agctgataaa agcaaaaaat
     2521 taatcaaata atcattaata tctatgtcct ggtacatatc caataataaa ataccaaatt
     2581 ttgctttact aaaatttaaa atgcgtggtt ttactggtcc atcaataaat ttggtaaatt
     2641 ctactgggca aaacttaact ttgagtttgg cagataaatg ttttgctaat ttatttccta
     2701 caacaatatt ccccatatat aaaaattaac tttgaatatt taataattct tgtatttttt
     2761 ctttgtcaaa tcccctaaca atttggccat caatatctaa tacaggtata cttgtttgcc
     2821 ctgttttttg tattaattca gtggcataat tgggattaga aacatctttt tcaatatatt
     2881 ctatgtgatg ttcatctaaa aaattttttg ctacatgaca ccagggacat cctggcgccg
     2941 tataaagtat aatcataata ataattttat ttttgtaatt ttttaataat ttttttaata
     3001 attttgattg ttgtttatat ctctattaag gcatcgcttg ctctattttc tgaataaaat
     3061 ctgacaatga tattgcccca acatcttgca tcctcccttc taccatcatt tgtattggtt
     3121 taccattcaa aaatgtcgtt gggaaaccta atgattgata tttatttaca tatcactgag
     3181 atctttctaa aataacatct cgatacttat gtgtatctaa acaattcttt aatttgtttt
     3241 gatctaaatt tatattgctt aaagtattta taatattatc ataagaaaaa tcaattgtat
     3301 tgtctggtgt ttgtttaagt tgtcaaagca tttcattggc ttcccaaaac ttaccttgtt
     3361 cattagcgca atacgccgct tctgaggcta acaacgaagg cgaatttata ttagcaaaca
     3421 aatcatatct caatgagaca tcatatttat ttatttcgct atccaaaaaa ggtttgatag
     3481 tctgatcaaa atattgtcta catcaaaaac attcataatc tgccaaaata acaatgtcta
     3541 atttggcatt tgttttgcca attggttcca aacttttggc tatttggcta ctttctattt
     3601 ctgtattagc tgtatttttc tttaaagaat tattaaaaaa tacactataa cccacaataa
     3661 ccattaacaa caaagctaaa atagaaatga tttttcattt taattttata ggatgtttta
     3721 tcataaaaaa tgttaaactt ttatataata atatattact ctataatatt atcaaattat
     3781 gtgatatttt gcaataacta ctactaaata tcgctaagat aactattaat gccgtctttt
     3841 tattgcttct tttatcgttt cgggatcagc aaattcaatt tcgccaccat aaggaatgcc
     3901 tacgcctatt tgagtaattt taatattata tggctctaat atctttttta aatacaaaga
     3961 agtcaaataa ccttcggcag ttgggtttaa ggccaaaata atttcttcaa tgggtttttt
     4021 attttgtatt gattctctga ctttaaaaat taactgctta atagtcaaag aattttcttt
     4081 ggcttcatca atattggaca gcaatccccc taatatatga tactcccctt tgtatttacc
     4141 aattttttct atggtaatta aatctgtatc attttctacc acacaaataa cattatgatt
     4201 tctgttagga tcttgacaaa taggacataa tatttggttt ttagtattcg gtgcaaaaag
     4261 aaaaaaacaa ttcgaacaaa tttttatatc ttgcgatata tttcaaatta attttgcaag
     4321 gtctttcaaa tttttttgtg aataatgagc aacaaaaaaa gacaaacgac ttgcttgtcg
     4381 cgatcccact cctggcaact tttgaaaaaa tcctgttaat ttatgtatat catcaggtat
     4441 aatatccata gtaatattaa aaaagattat ttagatataa ttttaatttt cttcaaaacc
     4501 ttcaccaaaa tcattatttt ctgtattatc tggtggtata agtggcgtat agccttcttc
     4561 tatttcttct gtcatatttt tatctaactc tcgaaaactc gtaatctctg gattaaaata
     4621 taattgtact tgaccaacag ggccatttct atgtttggaa atcaaaattt cagcaatatt
     4681 tggattggga ctatcctttt tatatttatc ttcgcgataa attaacatta ccacatcaga
     4741 atcttgctca atactattat gaacaattat atcattggct ataaaattat gtgtatcttg
     4801 cactgtaaca tcataaacat cttccatacg caacgataca atagatacaa tttcatctca
     4861 aataatttct ggactattta agtttttgtt aaaaacagct aatctatctc ctgtttttaa
     4921 ctcatctaat ctaactcagc catccaatgt tctaaaagga tgattggctg atgccttaat
     4981 agttctacca ctttttaaac ttaattcata aaccattttt ttaccactat aaaaagcttt
     5041 aataagtggt ttaacaacta attttcaatt atcatccaaa gtataaacag gaatttcaac
     5101 ttgttcttgt ctatcagcca gatctttaat ttttactact tcatctgtat cagcacgcat
     5161 aattaaacta tcaaatgcca aacatcctgt ttctcttaaa tcagataatt ttggaattgg
     5221 tggggtgcgt tgttcaacat ttcttgacaa ctgtgaaact actaaaattg gaa
//
LOCUS       DAOTJA010000044         5216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_14129_length_5216_cov_3.737066, whole genome shotgun sequence.
ACCESSION   DAOTJA010000044 DAOTJA010000000
VERSION     DAOTJA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14129_length_5216_cov_3.737066"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="P5241_03305"
     CDS             complement(<1..1172)
                     /locus_tag="P5241_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRV32539.1"
                     /translation="MKKGVFLFVVLVCFSVLFSQTTGYTEKVSDAAVLVENFTATGNL
                     NGYNSWTRYDGTADQIQITSGALTYSNYPNSGVGNKITINGSNSEDVYKSFTAITSQY
                     VYYSFLIKVTPSKSSGTYFMFLDPDGNLSTYNDWCARLFAKSDGTNIQFGISNSSTAS
                     WASTNYDNNTHCIVVKLDRNTGNLALFVDPDLSNSTEPTPTFTVNGSGAIGNVSSIAI
                     RQAPNLPAMDISNLRVANSWSQAPLPVELSLFTYKLVDGKVKLVWNTATELNNKGFYV
                     EKNTGNSWITLGFVDGKGNSNISNSYEFTDNSQNFGKVTYRLKQVDNDGKFTYSHTIE
                     VNATLPKELTLMQNYPNPFNPTTTITYAIPTAGNVSVKVYNSLGQEVVTLFEGYQEA"
     gene            complement(1192..1368)
                     /locus_tag="P5241_03310"
     CDS             complement(1192..1368)
                     /locus_tag="P5241_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32540.1"
                     /translation="MKKLFLTTLFLYFSSISIVVGAEKVKVEFIVDKDGTVKNPVVVI
                     RNNFTTYCKKLNIF"
     gene            complement(1743..2768)
                     /locus_tag="P5241_03315"
     CDS             complement(1743..2768)
                     /locus_tag="P5241_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF483 domain-containing protein"
                     /protein_id="HRV32541.1"
                     /translation="MNKSNKDKGHQSKKKCRYPAPKPTRKRIGDRVNEDDIVLKIGTG
                     SLKSGGMPGGFFWHIYYKDEKAGKIYINYDISSNRAEIQIFINQKSQGKGIGRVAYKR
                     ACELSQYSEIYATMRKSNTAFLKAAFAAGFRAIPSDSNQVQMLWSKLSFSIEAFERLP
                     EKLPRSSYVDFLLLLGNLKPALRIKISQNENIVDLSHWCETNDFYFLLGKDDYAFIAK
                     NKTIANNIEIIDISTSPHEYELGKMLGYPDCCCKKIAKICEENIDKYEDVFINEFNVK
                     NSYVLINPKGYKEGYALISHIPCSPTCEYSLRIAQKSFYIISRYNDRESFKIWVNAWK
                     EYFFLDY"
     gene            complement(2761..3786)
                     /locus_tag="P5241_03320"
     CDS             complement(2761..3786)
                     /locus_tag="P5241_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32542.1"
                     /translation="MKARVNVKLNSIFNLTNIDDLIFDHFSNHDIEIVENSHPFYELT
                     LERLPFLYCEDFTKGLDLFKIKEDEVLNFYNIDHKSIFTLLESWIPYGWSCFFAQRNE
                     IPKNLTIIHLDDHSDLMSPFISVDESKLWKDILTGCSINIMEPESIKMAIESGAITLG
                     SILTLLVFSVKNINIYHLKQNVKTTLKYIKKDIEVDPIIIPRQKKMSIKLLDDSYKYM
                     AENSKYLITSDVNKLIESVKDNYDIFLHIDMDFFNNRYNGSTDFTNYHDPDINHQKNI
                     IKIFCDAIAQSDLIDRIVHISIGLSPSFYPSEYWHDSLAALIYELKRNNFKIIPNIYI
                     ENRGNCE"
     gene            complement(3755..4924)
                     /locus_tag="P5241_03325"
     CDS             complement(3755..4924)
                     /locus_tag="P5241_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV32543.1"
                     /translation="MAMGNIQTFFLRVSARCNLNCSYCYVFKHNDKSWKSLPPIISEE
                     HVIIFANRIKEYLQNKTISCVNIIFHGGEPLLVGENLILKYIDIINTILSNLIKVQYS
                     IQTNGTLITENFIAEADRLNIKFSLSIDGPKQIHDKNRKKHNGKGSFEEVLKSIKILQ
                     KHPNLLQGIIGVIDVHSDPDEIFSFFKNNNIYNIDLLLPDANYDRPPIYRDTSPDIYK
                     NWLINSFNIWFNYYQDLSFGTYENILKGILGINELTDFFGGGELNYLTIETDGSYHTT
                     DILKVAYENASSIGMSLSNASIDSALKSPKVSEYNHLLKLDDLPTVCCTCEVKDICLG
                     GSLPHRYSSKNGFNNPTIYCSEVKALINHAKDKLIEQLEIEKEDLIESKSECKIE"
     gene            complement(4937..>5216)
                     /locus_tag="P5241_03330"
     CDS             complement(4937..>5216)
                     /locus_tag="P5241_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV32544.1"
                     /translation="LPYIEALPCCVFTDDTIEYALQFQKKIQLQQSSVVGYAGEKDKH
                     IDYGIVQKEGYSKYDSCIKCVYNNICLGVWKEYKKLYPNDEMYNLFNK"
BASE COUNT         1748 a          807 c          718 g         1943 t
ORIGIN      
        1 gcttcttggt agccttcaaa taatgttaca acttcttgtc ctaagctgtt gtaaactttt
       61 acgcttacat taccagctgt tggaattgca taagttattg ttgttgttgg attaaatgga
      121 tttggataat tttgcattaa tgttaattct tttggtaaag tagcatttac ttcaattgta
      181 tgtgaatatg taaatttacc atcgttatct acttgtttta atctgtaggt tactttacca
      241 aagttttgtg aattatctgt aaattcgtag ctgtttgaaa tgttggaatt acctttacca
      301 tcaacaaaac ctaaagtaat ccagctattg ccagtatttt tttctacata aaagcctttg
      361 ttgtttaatt ctgtagcggt attccataca agttttactt taccatcaac taatttgtag
      421 gtgaataatg atagttctac agggagagga gcttgtgacc agctattagc aacacgaaga
      481 ttggagatgt ccattgcagg taaatttgga gcttggcgaa tagcaataga tgatacattt
      541 cctatggcac ctgaaccatt aacagtaaaa gttggtgttg gctctgttga atttgagaga
      601 tcaggatcta caaataaagc tagatttcca gtatttctat ctaattttac aacaatgcag
      661 tgtgtgttat tatcataatt tgttgaagcc caagaagctg tactagaatt acttatacca
      721 aattggatat tagtgccatc acttttagca aataaacgtg cacaccagtc gttataagta
      781 cttaaatttc catcaggatc aagaaacata aaatatgtgc ctgaagactt tgaaggagtt
      841 actttaatta aaaatgaata ataaacatat tgtgatgtaa ttgcagtaaa tgatttataa
      901 acatcttcag agtttgaacc attaattgta attttatttc ctacaccaga attaggataa
      961 tttgaataag ttaatgcacc agaagtaatt tgaatttggt ctgcagtgcc gtcatatcta
     1021 gtccaactgt tataaccgtt taaattacca gttgcagtga aattttccac tagaactgca
     1081 gcatcagata ctttttctgt ataaccagta gtttgtgaaa atagtacaga aaaacaaact
     1141 aaaacaacga aaagaaaaac tccttttttc ataaaaactc ctttaaattt gttaaaaaat
     1201 atttagtttt ttacaataag ttgtgaaatt atttcttatt acaacaacgg gatttttcac
     1261 tgtgccatct ttatctacaa taaactcaac tttaactttt tcagctccaa caacgatgct
     1321 aatagatgaa aaataaagaa ataaggttgt taaaaataac tttttcatag tagaacctcc
     1381 atatttagat tgttaaaaat ttttaattct taaaaaacaa taatttgttt aataattttt
     1441 gtaaaatgtt gttaaaaaat tttataaaac tgttattatt tatttgtgtt tatggtgtaa
     1501 cctccatatt cattttaaaa aaaagttttt ttgcgaaagt aaaaagattt tattttgcaa
     1561 acttaatata gaaaataatt gttaagatac aataataatt ttgttatgta atgataaact
     1621 taaagtgata ccgtcggaca gtatatgacg gccggttctc cggctgtccc cgaagggcga
     1681 aacgaccgac agggagtggc gatatactgt ctgttagcgg ttggattttg gaactgttaa
     1741 atttaataat ctaaaaaaaa atactctttc caagcgttta cccaaatttt aaaagattct
     1801 ctatcattgt atcgtgaaat aatataaaaa gatttttgtg caatacgcaa tgaatattcg
     1861 caagttggtg aacaagggat atgagatatt aatgcatatc cttctttata acctttggga
     1921 tttattaaca catatgaatt tttaacatta aattcattaa tgaacacatc ctcatactta
     1981 tcaatatttt cttcacaaat ttttgctatt tttttacagc aacaatctgg atagcctaac
     2041 attttcccta attcatattc atgtggagat gtggatatat ctattatttc aatattatta
     2101 gcaattgttt tgttttttgc aataaaagca taatcatctt taccaagtaa aaaatagaaa
     2161 tcatttgtct cacaccaatg acttaaatct acaatatttt cattttgtga tatctttatg
     2221 cgtaaagcag gctttaaatt acctaaaaga agcaagaaat caacatagct tgatctgggc
     2281 agtttttcag gtagtctttc gaaggcttcg attgaaaagc ttaatttact ccacaacatt
     2341 tgaacttgat tcgaatcaga aggaattgct ctaaaaccag ctgcaaaagc agcctttaaa
     2401 aatgctgtat tactttttct cattgtagca taaatttctg aatattgact taactcacaa
     2461 gcacgcttat acgccactcg tccaattcct ttgccttggc ttttctgatt tataaatatc
     2521 tgaatttccg ctcgattact acttatatca tagttaatat agattttacc tgccttttca
     2581 tccttataat aaatatgcca aaaaaaccca cctggcattc caccagattt caaagatcct
     2641 gttccaattt ttaaaactat atcatcttca ttaactctgt cacctatacg ctttcgggta
     2701 ggttttgggg ctggatatct acattttttc tttgattgat gacctttatc tttattagac
     2761 ttattcacaa ttacctctat tttcaatgta aatatttgga attattttaa aattatttct
     2821 ttttagttca taaatcaatg ccgccaaact atcatgccaa tactccgaag gatagaatga
     2881 tggcgacaat ccaatactta tgtgtactat tctatctatt aaatctgatt gagcgattgc
     2941 gtcacaaaaa attttaatga tatttttttg atgatttatg tctggatcgt ggtagttggt
     3001 gaaatctgtt gagccattat atctgttatt aaaaaaatcc atatctatat gaagaaatat
     3061 atcataatta tcttttacac tttcaataag tttattgaca tctgaggtta taagatattt
     3121 actattttca gccatgtatt tatatgaatc atcaagcaac tttatggaca ttttcttttg
     3181 tcttggaata attattggat caacttcaat atcttttttt atatatttta atgtagtttt
     3241 aacattttgt tttagatgat aaatatttat attttttact gaaaagacca acaatgttaa
     3301 tatactacca agtgttatag ctccagattc aatggccatt ttaatgcttt ctggttccat
     3361 aatattaata ctacaccccg ttaaaatatc tttccagagt tttgactcat caacactaat
     3421 aaaaggagac attaaatcag agtgatcatc aaggtgaatt attgtgagat tctttgggat
     3481 ttcatttctt tgtgcaaaaa aacaactcca cccataagga atccaagatt caaggagtgt
     3541 aaaaatactt ttatgatcta tattataaaa attaagaact tcatcttctt taatcttaaa
     3601 cagatctaag ccttttgtaa aatcttcaca atataaaaaa ggtaaacgtt ctaatgttag
     3661 ttcgtaaaat gggtgactgt tttccactat ctcaatatca tggtttgaaa aatggtcaaa
     3721 tattaaatca tcaatattag ttaaattaaa aatactattc aattttacat tcactcttgc
     3781 tttcaattaa atcctctttt tctatttcta attgctctat aagtttatct ttcgcatgat
     3841 taatcaaagc tttgacctct gagcagtata tggttgggtt attaaagcca ttttttgaag
     3901 aataacgatg tggtagtgaa cctccaagac aaatatcctt aacttcgcaa gtacagcata
     3961 cagttggaag atcatctaat tttaataaat gattatattc actaacttta gggcttttta
     4021 atgcactgtc aatacttgca ttagacaatg acatgcctat tgacgatgcg ttttcatagg
     4081 caacttttaa aatgtctgta gtatgataac taccatctgt ttctatggtc aaataattca
     4141 actcaccacc accaaaaaaa tcagttaact cattaattcc caatatacct tttaatatat
     4201 tctcataagt accaaatgat aaatcttgat agtaattaaa ccatatatta aatgaattaa
     4261 ttagccaatt tttgtatata tcaggcgaag tatcacgata aattggtggt ctatcataat
     4321 tagcgtctgg taataataaa tctatattat aaatattatt atttttaaaa aaactaaata
     4381 tttcatctgg atcagaatgg acatcaatta cacctatgat tccttgaagc aagttaggat
     4441 gtttttgtaa aattttgatg ctttttaaga cttcttcaaa tgatcctttt ccattatgtt
     4501 tttttctgtt tttatcatga atttgtttag gaccatcaat acttaatgaa aattttatat
     4561 taagcctatc agcttcagca ataaaatttt ctgtaataag tgtgccattc gtttgtatag
     4621 agtattgaac tttaataaga ttacttagta tggtattaat aatatcaata tatttcaaaa
     4681 ttaaattttc gccgacgagt aaaggttcac caccatggaa tattatattg acacagctaa
     4741 ttgttttgtt ctgcagatat tcctttatcc tatttgcaaa aattataaca tgttcttctg
     4801 aaattatagg aggtaaactt ttccatgact tatcattatg tttaaaaaca taacaataac
     4861 tacagtttag attacagcgt gctgatactc gcaaaaaaaa tgtttgtata tttcccattg
     4921 ccattatcct ttatttttat ttattaaaaa gattgtacat ttcatcattt gggtataatt
     4981 ttttatattc tttccatacc cctagacaaa tattattata tacacatttg atgcaagaat
     5041 cgtattttga gtatccttct ttttgcacaa taccatagtc aatgtgctta tccttttctc
     5101 cagcgtaacc aacaacactt gattgttgta actgaatttt tttttgaaat tgcaaagcat
     5161 actctattgt atcgtcagtg aaaacacagc atggtaatgc ttcaatatat ggaagt
//
LOCUS       DAOTJA010000045         4971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_14867_length_4971_cov_5.698332, whole genome shotgun sequence.
ACCESSION   DAOTJA010000045 DAOTJA010000000
VERSION     DAOTJA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14867_length_4971_cov_5.698332"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="P5241_03335"
     CDS             <1..479
                     /locus_tag="P5241_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32545.1"
                     /translation="GILKEKENNENERIRSLILSKIREKIKIEPPESLVTRELDYMLE
                     DIKHRIMHLGFTFEDYLKQINKTEDKIREELTKEAKQKVLDALIVREIVKRENIVIEP
                     KELNDKINELMIALSYQVEDPTKINKESIKSYAQELCENEKVFDLLLKGDSKGDLK"
     gene            680..1297
                     /locus_tag="P5241_03340"
     CDS             680..1297
                     /locus_tag="P5241_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRV32546.1"
                     /translation="MNKITNNSDQLIPMVVEKSNQGYERAYDIYSRLLKERIIFLAGP
                     IDDNVANIVIAQMLFLEFEDPKKEITIFINSPGGAVNAALAIYDTMQYVKPDIKTVCV
                     GIAASAATLILCAGTKGKRFSLPNSEVLIHQVMGEAGGQATEIEISAKHILKIKDRLN
                     KILSKHTGKTIAQIEKDSDRDHWFTAEEAKDYGLIDDIIRTKTIK"
     gene            1494..1582
                     /locus_tag="P5241_03345"
     tRNA            1494..1582
                     /locus_tag="P5241_03345"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1528..1530,aa:Ser,seq:gct)
     gene            2001..2288
                     /locus_tag="P5241_03350"
     CDS             2001..2288
                     /locus_tag="P5241_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRV32547.1"
                     /translation="MILPELGINITPILASLGIVGLAVGMAAKDIIADFISGIFILLE
                     GQYYIGDEVNISNIEGQVQEITLRKTILKDKDNMIHIIPNSQIKIVSRKSD"
     gene            2363..2698
                     /locus_tag="P5241_03355"
     CDS             2363..2698
                     /locus_tag="P5241_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32548.1"
                     /translation="MKKINNTLDKTILRVSLIFGFIIAISIVIYLQTNGKLLDTGSVN
                     YNIENKEQTVIKDLVEKFGQSFKNVYLSSPDVKDTLAQAYQDFVSPELLEQWQQNPSQ
                     APGRIVSSP"
     gene            3017..3235
                     /locus_tag="P5241_03360"
     CDS             3017..3235
                     /locus_tag="P5241_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32549.1"
                     /translation="MLKFDFFSRNISKKIPENTSKKIQKENIAEQNIEKGEKKIKDIA
                     SLSEKDKQELKDAISKSNGLIRLFVHPY"
     gene            3251..3406
                     /locus_tag="P5241_03365"
     CDS             3251..3406
                     /locus_tag="P5241_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32550.1"
                     /translation="MDQIQSIEIPNTNIDIYNDVKILQTKFEEIITKPSFNMPPIFVM
                     ERYNNLA"
     gene            3644..3826
                     /locus_tag="P5241_03370"
     CDS             3644..3826
                     /locus_tag="P5241_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32551.1"
                     /translation="MNLDIPKSKKSNSYILQCVGDVVKYLKQLGIECEVSLLVAPQQL
                     RQSFIERNVKNNNSTT"
     gene            3933..4014
                     /locus_tag="P5241_03375"
     tRNA            3933..4014
                     /locus_tag="P5241_03375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3965..3967,aa:Ser,seq:cga)
     gene            4178..4900
                     /locus_tag="P5241_03380"
     CDS             4178..4900
                     /locus_tag="P5241_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRV32552.1"
                     /translation="MQQNIQKNRKKLLVFASGGKEPNEGGSGFQEMVEFSRTDPPILD
                     ADIVAVISNNQFGGVYNKSQLLNIPFEYWSGPFDEEGYKTIIEKYQPDFVMLSGWLKK
                     TIGLNPSKTINIHPGPLPEFGGKGMYGHFVHEAVIEAFHQGKIKQSAISMHFVTEKYD
                     EGPIIVKYPVLIRLDDTPQTLAQRVNEKERVIQSIFLNLVVMGYITYPDPDYRMLVID
                     EKIWGFYKQFANIPGQKYEIKK"
BASE COUNT         1926 a          597 c          785 g         1663 t
ORIGIN      
        1 atggtatact aaaagaaaaa gaaaataatg aaaatgaacg aataagatca ctgatattaa
       61 gcaaaattag agaaaaaatt aaaatagaac cacccgaaag cttagtaaca cgtgagttag
      121 attatatgtt agaagatatc aaacatagaa taatgcatct tggatttacc tttgaagatt
      181 atttaaagca gataaacaaa accgaagata aaattcgcga agaattaacc aaagaggcaa
      241 aacaaaaagt gttagatgcg ttgattgtta gagaaatagt aaaaagagaa aatattgtta
      301 tagaaccaaa agaattaaat gataaaatta acgaattaat gattgctttg tcgtatcaag
      361 tagaagatcc caccaaaatt aacaaagaat ccataaaaag ttatgcccaa gaattatgcg
      421 aaaacgaaaa ggtatttgat ttattgctaa aaggtgattc aaaaggtgat ttaaaataat
      481 tttgtaatgt tttaaaataa ggttataatg ttttaaaata aggttataat gttttaacaa
      541 taaatatcca agatcgtgct gtgtcttatt gtgattattc atcattttga ttctatttgt
      601 gtatattggt gtttattata ttgtttatgt ttagagctat atttttaata aaacattaaa
      661 aaacattaaa tttaatatta tgaacaaaat aacaaataat agtgatcaat taatcccaat
      721 ggttgtagaa aaaagcaacc agggatatga aagagcctat gatatttatt ctcgtttatt
      781 aaaagaaaga attatctttt tagctggtcc cattgatgat aatgtggcga atattgttat
      841 tgcccaaatg ttgtttttgg aatttgaaga tcctaaaaaa gaaattacta tatttatcaa
      901 ctcaccagga ggagctgtta atgctgcttt agctatttat gatacaatgc aatatgtaaa
      961 accagatatt aaaacggttt gtgttggtat tgctgcttct gctgctacat tgattctttg
     1021 cgcagggaca aaaggtaaaa ggtttagttt gccaaactct gaagtactta ttcatcaagt
     1081 tatgggtgaa gctggtggtc aagcaacaga aatagaaatt tctgctaaac acattttaaa
     1141 aattaaagat agactaaata aaattttatc taaacacaca ggcaaaacta tagcccaaat
     1201 tgaaaaagat tctgatagag atcattggtt tactgccgaa gaagcaaaag attatggttt
     1261 aatagatgat attataagaa caaaaactat taagtagaag ataataaatt tttaatggct
     1321 aatttaatgg ctcgcatacg cgagccatta caatatcgga taagtttaaa cacatttgat
     1381 actttataga accctaaata ggtaaaacgc atacaatatc ttgtatattt tgtgattttg
     1441 tgataagatt aaaataatac ctaacattta tgtatatgga gttttataaa tatggaggcg
     1501 tgactgagtg gtttaaagta acggtttgct aaaccgtggg ggtctaaaaa gccccgcgca
     1561 ggttcaaatc ctgtcgcctc cgcttatttt tataggttag acctcggtgt ttaaaatcta
     1621 aaaaatctaa aaagttgatt gagattttta atttatatat ttgatctaaa tactaaatag
     1681 aaattaaaaa tcccaattgc caaaattcat attttatagt acaatgttcg tatcttatta
     1741 tatattaatt taattttatg aacaaatata gtattttgga aaaattattg gaatggtttt
     1801 tagcacacgg aattaaagtt cttggtattt taattgtggc tagtttagtt gattgatttt
     1861 taaagatttt tattactaaa tttattcaga atctagtcaa agaaaaggtt tcagaagaac
     1921 gaaaaaagaa aaaagtagcc acattaatta gtacttttta tggtgctatt aattttatta
     1981 tttgatctat agctttgtta atgattttac cagagcttgg tataaatatt acaccaattt
     2041 tggcaagttt gggtattgtt ggtttggctg ttggtatggc tgccaaagat attattgcag
     2101 attttatatc aggaattttt attctgttag agggacaata ttatattggt gatgaagtaa
     2161 atatttctaa tatagaaggt caagtccagg aaattactct acgcaagact attttgaaag
     2221 ataaagataa tatgatccat attattccta atagccaaat taaaatagta tctagaaaat
     2281 ctgattagaa ttttttaata tgtttaaagt gttactataa tatcaacctt taagtaagaa
     2341 gtaatttgtc aaataaattg ttatgaaaaa aataaataat acattagata aaacaatact
     2401 tcgtgtttct cttatatttg gttttattat tgctattagt attgtaatat atttacaaac
     2461 aaacgggaaa ttattagaca ctggatcggt aaattataac atagaaaaca aagaacaaac
     2521 agttatcaaa gatttagttg aaaaatttgg gcaatccttt aaaaacgtat atttgtcttc
     2581 tcctgatgtc aaagatacac tcgctcaagc ttatcaagat tttgtaagtc ctgaattact
     2641 tgaacaatgg caacaaaacc catcccaagc gcctggtaga attgtatcaa gtccttgacc
     2701 tgacagaata gacattacaa gtatcaaaaa aatagacgaa gttacctata ttatatatgg
     2761 tgaaattatt gagatcacca gtgttgaaca agttcaagga ggaattgccg ctaaacgtga
     2821 tataattttg acaattacaa aaataaataa tagttggctt attagtgatg ttatattggg
     2881 tgtatacaaa taaaatattt tagatatatt attggttatt attttttatg attgattgtt
     2941 ttatttttaa aaattaagaa aatgtgttat aattattata acgtaattta aaaataaata
     3001 ataaaatcac tataatatgt taaaatttga ttttttctca aggaatatat caaaaaaaat
     3061 accggaaaat acatcaaaaa aaatacaaaa agaaaatata gcagaacaaa atattgaaaa
     3121 aggagagaaa aaaattaaag atattgcatc gttatctgaa aaagacaagc aagaattaaa
     3181 agatgctatt agcaaatcta atggtttaat tagacttttt gttcatccct attgagtaga
     3241 cccgagtaaa atggatcaaa ttcaatcaat agaaatacca aatacaaata tagatatata
     3301 taatgatgtc aaaattcttc aaacaaaatt tgaagaaata ataaccaaac cttcttttaa
     3361 tatgccgcct atttttgtta tggagcgtta taataacctt gcttgattta aaaaaacatt
     3421 agaagacaaa attcaacatc caatttatct tgtaccgacg agcaatatta gtgtcgagcc
     3481 aatagatttt ttgggcaatc aaacacaaaa aaacttttga aattcgttaa cacaatttgg
     3541 agaagacgca tttcaattac ccccagcaat acctacaaac aatatacaag gttttgcaga
     3601 atacttaaaa aatttaggtg taacaaaagc tcttattgga ggtatgaatc ttgatatacc
     3661 aaagtccaaa aaatccaatt cctatatttt acaatgtgta ggtgatgtgg ttaagtatct
     3721 taaacaatta gggatagaat gtgaagtttc tcttttggtt gcacctcaac aattacgtca
     3781 atcatttatt gaacgaaatg tcaaaaataa taattctaca acttaaataa tttatttttt
     3841 caaaatagtc attgttttta attagtaact catttttgtt ttgaaattat ttaaaattgt
     3901 taataatttt ttataatatc atataatatc acggagggtt ggcagaatgg tattgcgtcg
     3961 ctctcgaaaa gcgatggacg aaagtccttg caggttcgaa tcctgtgccc tccgctacca
     4021 atcagcaatt aacaattggc aattgacatt tagcaattag tttttgtact attaaacttt
     4081 tgaactatta cttttttaat aataaatata tgctacaatg aggagtgata ggtttaaaat
     4141 tggaggctaa atttaaaaaa ttaaagaaga aaataaaatg caacaaaata tacaaaaaaa
     4201 tagaaaaaaa ttattagttt ttgcttctgg aggcaaggag ccaaatgaag gtggtagtgg
     4261 atttcaagaa atggtagaat tttcaagaac tgatccacca attttagatg cagatattgt
     4321 agctgttatt tctaataatc aatttggtgg tgtttataat aagtcgcaat tacttaacat
     4381 cccatttgaa tattggtcag gcccatttga tgaagaaggt tataaaacta taattgaaaa
     4441 atatcagcct gattttgtaa tgttatcagg ttggttaaaa aagactattg gtttaaatcc
     4501 atccaaaact attaatattc atccgggccc attaccagaa tttggtggca agggtatgta
     4561 tggacatttt gtacacgaag ctgttattga agcttttcat caagggaaaa taaaacaaag
     4621 tgcaatttca atgcattttg tgacagaaaa atatgatgaa ggtcctatta tagtaaaata
     4681 tccagttttg attcgtttag atgatactcc ccaaactctt gcccaaagag ttaatgagaa
     4741 agaaagggtt atacaaagta tatttcttaa tctggttgtt atggggtata ttacttaccc
     4801 tgatccagat tatcgaatgc ttgtaattga tgaaaagatc tgggggtttt ataaacaatt
     4861 tgccaatatc cctggtcaaa aatacgaaat taaaaaataa tatattacat atttggtttt
     4921 ttaattttaa tcgttaatta tgaataaata taaaaataaa aaaatagtta t
//
LOCUS       DAOTJA010000046         4819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_15400_length_4819_cov_4.639169, whole genome shotgun sequence.
ACCESSION   DAOTJA010000046 DAOTJA010000000
VERSION     DAOTJA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15400_length_4819_cov_4.639169"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..2182)
                     /gene="ftsH"
                     /locus_tag="P5241_03385"
     CDS             complement(374..2182)
                     /gene="ftsH"
                     /locus_tag="P5241_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRV32553.1"
                     /translation="MTKLFTNLLVVFMIMLGVIYLFSTLDQINITRREVSLNELVSNI
                     ASQKIQKVIVKPDSIIGVIDDKNELISKKETNISFFETLKYYELPTSTLQKLDIQFKE
                     NTDWASILTIGTMVILPILFMVGFFMFISRQAKQGPGQIFSFSQANIKQFSPSKDRIT
                     FKDVAGLKEAKEELEEIVEFLKTPKKFTDLGAKIPRGVLLMGPPGSGKTLLARAVAGE
                     SGVPFFYISGSEFVEMFVGVGSARVKSAFAIAKKAAPSILFIDEIDAIGRERGSGIGG
                     GQDEREQTLNQILVEMDGFDKDTHVIVMAATNRPDVLDPALLRPGRFDRRIVLDLPDK
                     EERKQILQIHLRNKKFDHNIDLDKVAMRTPGFSGADLENLINEGAILAVRKNKNKLEE
                     TDLLESIEKVMMGPERKSHVMNKHEKEITAYHESGHAIVAAKLPHANPVHKISILPRG
                     IAAGYTLQLPEEDKYLKSKSEFLDELAVMLGGYVSEEQHFGEVTTGSSNDLKYASNSA
                     RAYVTKYGMSKLGPVSFVEEESIFLGKELATRKVFSEMMAAKIDEEVNRLISDARKKA
                     ESIIIKNKNLVDKIAQVLLEKEIIEKEEFYSYLKTT"
     gene            complement(2381..2509)
                     /locus_tag="P5241_03390"
     CDS             complement(2381..2509)
                     /locus_tag="P5241_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32554.1"
                     /translation="MFSPKTPTAKTFKEQEILGVKVRYFAYPEKEAALVYAITDHY"
     gene            complement(2552..2992)
                     /locus_tag="P5241_03395"
     CDS             complement(2552..2992)
                     /locus_tag="P5241_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32555.1"
                     /translation="MFKPKNLTSNIPGITSPVVSSPLQPTTSSTTLPALNFSKTKGDV
                     INYKLDIDITGAPIILSTIKKEAEVLGTPNQIKIIEPTVKGQKLSSKEVLHTLIKNVP
                     QVLSDAIKTDYLVYTYYGEINPALGLIFDIDPSKLQDLTESMLL"
     gene            complement(2999..>3335)
                     /locus_tag="P5241_03400"
     CDS             complement(2999..>3335)
                     /locus_tag="P5241_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32556.1"
                     /translation="NYQTFYSTPQTPMATPIEPLSSFPPNSPASSQPSQPNDNIMFNP
                     SLSPELSNVSSNIPMQDNQSMGQFNVATEPSLEASSIPTYTKPKTNTKKLLIPIIIII
                     VLLGFYFLI"
     assembly_gap    3336..3435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3436..3686)
                     /locus_tag="P5241_03405"
     CDS             complement(<3436..3686)
                     /locus_tag="P5241_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32557.1"
                     /translation="MADISQNPQSNIKPDESNIYIRTMNQDMQNLNQGGGNFSPGLGA
                     QQQSIPNTSQVPSPTPSQTPTSFDQSSLNELKQKIQNINS"
     gene            complement(3849..4385)
                     /locus_tag="P5241_03410"
                     /pseudo
     CDS             complement(3849..4385)
                     /locus_tag="P5241_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(4442..>4819)
                     /locus_tag="P5241_03415"
     CDS             complement(4442..>4819)
                     /locus_tag="P5241_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV32558.1"
                     /translation="ADTVGDDEYKNALLHINQNISRGMTVGEAFKQEKIFPTILSNLL
                     SMGEKAGHVSEILATLSNFYKKELENQLKSLVALIEPVILIFMGVVVGGIALSVIMPM
                     YQMIGNMGNVEGTLPEGEISQGL"
BASE COUNT         1544 a          775 c          664 g         1736 t
ORIGIN      
        1 tgggtatcct gagccttatt gattgtttta tttggtctta gaataagata tactatacct
       61 gctaatatag ctaatataac aataactatc attagctcta ctagagtgaa actatatact
      121 actttattat tgtttaatct tttcttgttc atattatatt tttgatctat tttatttata
      181 ttgtgttttt ttatttattt tatttctatc aatgtatttg ttgatttttt gtaataatag
      241 cataatatag gggggatttg taactatttg cctcgggaaa gagtgggaaa tagagattgt
      301 aggttaaaaa agtaaaaatt gtttcttatt atatacatta atccacacct tgatctaagt
      361 aaatctttgt tttttaggta gttttaagat aactataaaa ttcttctttt tcaataatct
      421 ctttttctaa taatacttgg gctattttat ctaccaaatt tttatttttt ataattatac
      481 tttctgcttt tttacgagca tcagaaatta aacgattgac ttcctcatca atttttgcag
      541 ccatcatttc agaaaaaact ttacgcgttg ccaattcttt accaagaaaa atagattctt
      601 cctcgacaaa tgaaacagga ccaagcttgc tcatcccata ttttgtcaca taagctcgtg
      661 cgctattaga agcatatttt aaatcattag aagaaccagt agtcacctct ccaaaatgtt
      721 gttcttctga cacataacct cccaacatta cagccagttc atctaaaaat tctgattttg
      781 attttagata tttatcttcc tctggcaatt gtaaagtata acctgctgct attccacgtg
      841 gtaaaataga aattttgtgt acaggattag catgaggcaa tttagccgcc acaatagcat
      901 gccctgattc atgataagcc gtaatttctt tttcatgttt attcataaca tgactttttc
      961 tttctggccc catcattact ttttcaatag attctaacaa atcagtttcc tctaatttat
     1021 ttttattttt tctgacagcc aaaatagctc cttcgtttat aagattttcc aaatcagccc
     1081 cagaaaaccc aggtgtacgc atagccactt tatctaaatc aatattgtga tcaaattttt
     1141 tatttcttaa atgaatttgt aaaatttgtt ttcgttcttc tttgtctggc aaatccaaaa
     1201 caattctcct gtcaaatctt ccaggacgta ataaagctgg atccaaaaca tcaggtctat
     1261 tagtagctgc cataactatc acatgggtat ctttatcaaa accatccatt tccaccaaaa
     1321 tttgatttaa tgtttgttct ctttcatctt gccctccacc aatacctgat cccctttctc
     1381 taccaatagc atcaatttca tcaataaata atatagaagg tgcagctttt ttagcaatag
     1441 caaaagcgga ttttacacga gccgaaccaa caccaacaaa catttcaaca aactctgaac
     1501 cagaaatata aaaaaacgga acaccacttt ctcctgccac agctctcgct aacaaggttt
     1561 taccagaccc tgggggcccc attaacaata ctccccgagg aattttggct cctaaatctg
     1621 taaacttttt gggagttttc aaaaattcta caatttcttc caattcttct ttggcttctt
     1681 taagtccagc aacatcttta aatgtaattc tgtctttact gggcgaaaat tgttttatat
     1741 tggcttgtga aaaactaaaa atttgcccag gaccttgttt ggcttgtcga gatataaaca
     1801 taaaaaatcc aaccataaaa agtattggca gaattaccat agtaccaata gtcaaaatac
     1861 tagcccaatc tgtattttct ttaaattgaa tatctaactt ttgtaaagtc gaagtaggca
     1921 gttcataata tttcaaagtc tcaaaaaatg aaatatttgt ttctttttta cttatcaact
     1981 catttttatc atcaataaca ccaattatag aatcgggctt tactataact ttttgaattt
     2041 tttgcgaagc aatattagaa accaattcat tcaaagaaac ttctcgtcga gttatattta
     2101 tttggtccaa ggttgaaaat aaataaatta ctcctaacat tatcataaac actactaata
     2161 aattagtaaa caattttgtc attactataa ttttaaattt gtaaattaaa tatttttata
     2221 gaaatattat ttcaatctta aattattaag gcttagtagt aggcaatacc ggcacttctg
     2281 gtataactaa tttagttgtg ctcgtagtag tattactagc catacctact gataaatgat
     2341 ctatggcact cgtcattgat tccagtgatg ttgttattat tcaataatga tctgtaatag
     2401 catataccaa agcagcttct ttttctggat aagcaaaata cctaactttt actcccaaaa
     2461 tttcttgttc tttaaaagtt ttggcagtag gagtcttggg agagaacaat cacaaattaa
     2521 tagaatcttt tatcaatgct ttattttttt ctcataaaag catagattcg gtaagatctt
     2581 gtagtttaga cggatcaata tcaaatatta aaccaagagc aggatttatt tcaccataat
     2641 atgtatacac taaataatca gtttttatgg catcagataa aacctgagga acatttttta
     2701 tcaaagtgtg taaaacttct tttgatgata gtttttgtcc tttgacagtt ggctcaataa
     2761 ttttgatttg atttggagta cccagcactt cggcttcttt ttttattgta cttaatatta
     2821 tgggagctcc tgtaatatca atatctaatt tataatttat tacatcccct tttgttttag
     2881 aaaaatttaa tgctggcaac gtagtagaag aagttgttgg ctgtaatggt gaactaacca
     2941 ctggtgaagt aattccggga atattggaag ttaaattttt gggcttaaac aacttgggtc
     3001 aaataagaaa ataaaaaccc aacaatacta taattataat tataggaatc aaaagctttt
     3061 ttgtattggt ttttggttta gtataagtag gtattgatga agcttcgagt gatggctcag
     3121 tggcaacatt aaattggccc attgattgat tgtcctgcat aggaatattc gacgacacat
     3181 tagataattc aggagataat gacggattaa acataatatt atcatttggt tgtgatggtt
     3241 gtgaagatgc cgggctattt ggaggaaaag atgacaatgg ctctatgggg gtagccattg
     3301 gagtttgagg tgtagaataa aaagtttgat aatttnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnngaatt tatattttga attttttgtt ttaattcatt aagcgaagat
     3481 tggtcaaacg aagtaggtgt ttggcttggt gttggacttg gtacttgact tgtattaggt
     3541 attgactgtt gttgcgcgcc taacccagga ctaaaattac cacctccctg gtttaaattt
     3601 tgcatatctt ggttcatagt acgaatataa atattggatt catctggctt aatattagat
     3661 tgtggatttt gtgaaatatc tgccataata ataatttaaa tatttattaa tataattgtt
     3721 gattaaaaat attcgcgact ttaattagtt taatatgatt tgaatataat aatataatta
     3781 tacgccattt tttgtttttt gtcaattctt taaaaaaagt tttccacagc tcctttgtat
     3841 ataattttct atcgagattt agttaatttt ggtacacaat aatcagttga cgaagtactt
     3901 attattaaac ttcttgatat atcaaaagcc gcagaacgta tagtaatagt ataggtttga
     3961 tcacaaattt tagaattatt atcatcacac aatagtttag gactcaagtc agaactcgct
     4021 cctaaaattg ttggtgcata acaaaaacgc aaatctaaat tattacctga tgtcggcgaa
     4081 gtaaacacaa cattatctaa caaataaaat ttatctattg tttcagcact cggagttcca
     4141 atataagacg cataataatg tttatcagaa gttgtattag caaatcgaat tatatatata
     4201 ttatcagtag aggtagcaaa tatctcattt ttttcttgta catattgaaa aaaatctata
     4261 aaatcagtaa tagtattatc aagtgttaaa cccatagctg gttttaacaa taaatccaaa
     4321 gataaccctg caataaatgt cataattgtt attgtaatta ataattctac taatgtgaat
     4381 cctttttgtt tatttgtttg tcaattttta aacataatct taaaatgata tattaaaaat
     4441 tttacaatcc ttgagatatt tcaccctctg gtaatgtccc ctcgacgttg cccatattac
     4501 caatcatttg atacattggc ataattaccg acaaagctat accaccaaca actactccca
     4561 taaaaatcaa aatcactggt tctattaaag caacaagaga ttttaattgg ttttctaatt
     4621 ctttcttata aaaattcgac aaagttgcca aaatttcgct tacatgtcca gctttttctc
     4681 ccatagacaa tagattactc aaaatagttg gaaaaatttt ttcttgctta aatgcctcac
     4741 ccacagtcat acctctactg atattttgat ttatatgtaa caaagcattt ttatactcat
     4801 catcccctac tgtatcagc
//
LOCUS       DAOTJA010000047         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_15436_length_4810_cov_5.749737, whole genome shotgun sequence.
ACCESSION   DAOTJA010000047 DAOTJA010000000
VERSION     DAOTJA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15436_length_4810_cov_5.749737"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..903)
                     /gene="pilM"
                     /locus_tag="P5241_03420"
                     /pseudo
     CDS             complement(1..903)
                     /gene="pilM"
                     /locus_tag="P5241_03420"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
     gene            1423..1758
                     /locus_tag="P5241_03425"
     CDS             1423..1758
                     /locus_tag="P5241_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRV32559.1"
                     /translation="MHYLKERKRGFNQSRLLAQELIQYIPLEYNDKILIRIKNNQAQA
                     KIKNRQQRFQNAQNIFSVNKEFISTIKNKNVILVDDIYTTGATINSAAIVLKQSGAKN
                     VVALCLAKE"
     gene            complement(1786..1859)
                     /locus_tag="P5241_03430"
     tRNA            complement(1786..1859)
                     /locus_tag="P5241_03430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Arg,seq:cct)
     gene            complement(2215..2288)
                     /locus_tag="P5241_03435"
     tRNA            complement(2215..2288)
                     /locus_tag="P5241_03435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2252..2254),aa:Val,seq:tac)
     gene            complement(2528..2599)
                     /locus_tag="P5241_03440"
     tRNA            complement(2528..2599)
                     /locus_tag="P5241_03440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2565..2567),aa:Ala,seq:ggc)
     gene            2932..3003
                     /locus_tag="P5241_03445"
     tRNA            2932..3003
                     /locus_tag="P5241_03445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2964..2966,aa:Phe,seq:gaa)
     gene            3124..3396
                     /locus_tag="P5241_03450"
     CDS             3124..3396
                     /locus_tag="P5241_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32560.1"
                     /translation="MIPRLKYQKINIPTNNSAIEGTVLAKDKKALYVDASPLGLAIVR
                     GYEFNAARNFIKDLNIGDKVIIKILELDNELGLIEASLKEASRDKI"
     gene            3709..3939
                     /locus_tag="P5241_03455"
     CDS             3709..3939
                     /locus_tag="P5241_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV32561.1"
                     /translation="MGDVVECTITKIVPFGMFVKFNNPPIDGLIHISEIDYQLIANPA
                     DIYKEGEKIQAKIQSITNNKVSLSIKALKPDP"
     gene            3955..4170
                     /locus_tag="P5241_03460"
     CDS             3955..4170
                     /locus_tag="P5241_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV32562.1"
                     /translation="MYKAGKQYEGKILKTGTLGVLVEFEPGIYGFMKYNDVENKTKID
                     QPLEIGEKRNFTIKTIDPNTRRIALTL"
     gene            complement(4285..4557)
                     /locus_tag="P5241_03465"
     CDS             complement(4285..4557)
                     /locus_tag="P5241_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRV32563.1"
                     /translation="MKKEDLVELVHAKTQMTKKEAEDIINGIFDSIAKALKKGEDTAI
                     SGFGIFTVKSKAARVGINPKTGEKINIAAKKVAKFRPAKALKELIK"
BASE COUNT         1819 a          783 c          590 g         1618 t
ORIGIN      
        1 aaaatcatct ataataccag gcaaattagc tatgccacca gttaaataca ttttttctac
       61 tattctattt gtttttttat taaaattcga aatttccctt aaaatctctt gctttattac
      121 accaagcaaa ggcaacataa tccttgtaat atcttcttgc ccacgtggag atgccaaacc
      181 ataaagacgt ttaatttctt ctgctcgtat tggagaaatc gccaaaccag tcgcgactac
      241 ttgcgtcaaa tctccaccag ctgtatcaat agtacgcatt agttgtggta taccttcatc
      301 tacaatagca attgatgtaa cacgcccccc tatatctacc aatgccacca atcccctttc
      361 gttttttaat aaagaacgaa cttgactaac aacttctaat tctatagatt taatttttat
      421 attagcaagc ctacctattg ttgtatatgt tgcaataata tcttttggaa cagcgctcaa
      481 taaaacataa agccttgtat ctgtttgttt aacaacttgt caatctaaaa taacatcatt
      541 caaagatata gggacatact gtcttgcttg ataatgtact gcctgttcca attcttttcg
      601 gtttaaaata ggcaattcta ttaagttcat ataagtataa aacggaggta cagaaatatt
      661 ggcctcgcgg ctttttattt tactctgttt caataaatta gtcaaatact gagctgtatt
      721 tttttctaca attttaaaac tgctagtttg aatagcttcg ttaattcttt cgagatgtgc
      781 atagttttct aaaataccat aaccagataa ttttgccaca cctccagtct gaaccaattc
      841 tactactttt attgaggtag taccaatatc aatacctaat cctacttttg aaaaaagacc
      901 catagctata ttttaaaata ttttgattta tgatataata tacaaaagta taacaaccaa
      961 atgataatac ataataaatt actatattaa ttatatctaa tttttataaa aaaagcaata
     1021 gctatgaaca tatttaaaca taaaacacaa atacctaaat tagttttaaa cactttgttt
     1081 ccttgaaaat gtttagtttg ttttaaatat acagacaacc cctatccact ttgcgataat
     1141 tgtcttaaac aaatcaaaac aataaattat ttttgttgtc caatatgcaa caaacgaata
     1201 agcccaaaca aattccaata ttgtcatagc aaagaaaata aaattcttgc ctatgccgct
     1261 cccctttctt tttcagatac aaatatcaaa aatttaattt attactttaa atatcaaaat
     1321 attactagtc tatccaaacc attagctaca tttttactta tgcccttagt acaatataaa
     1381 aatatattta gaaatactga atgaacaatt atacctattc ctttgcatta tcttaaagaa
     1441 agaaaacgtg gttttaatca gtcccgtctt ctggcacaag aacttataca atatattcca
     1501 ctcgagtaca acgacaaaat attaattaga ataaaaaata accaagcaca agctaaaatt
     1561 aaaaatagac aacaacgttt tcaaaatgct caaaatatct tttctgtaaa taaagaattt
     1621 atatctacaa tcaaaaataa aaatgtcatt cttgttgatg atatttacac tactggagct
     1681 actattaatt ctgctgccat agttctgaaa caatctggtg ccaaaaatgt ggttgctcta
     1741 tgtttagcca aagaataact aatattttaa aaaagaattt tattgtgtcc ctggtaggag
     1801 tcgaacctac aatctactcc ttaggacgga gccgctctat ccagttaagc cacagggaca
     1861 atgatatttt aaataccaaa tattaatagt acataggttt taacttatag tgtaaagctt
     1921 tataaatata taaatcattt atcctttttc cataaaacat ggactcttac aaacaccttt
     1981 atacaatata caataatcaa acactaatat tcaagcacca aataatacaa atagatatat
     2041 cattgcggga agtaaaaacg aacccgcacg gcgaatacga aagacgaaac aatctcaaca
     2101 ttaattgtat gcaatataaa taaaattatt tttaactttt aacctctatc ttgtattatt
     2161 atttaaattc tctgactttt aacttacaac ccttgaactc tgttattagt ttagtggacg
     2221 cgacaggatt cgaacccgtg acctcttgaa tgtaagtcaa gcgctctaac caactgagct
     2281 acgcgtccaa tatccaactt ttcctgtcaa attttgtttt aattgatata tacgcaataa
     2341 taattgtatg taataatgtc tacttaaata aattatattg taaaatctat tagacaacaa
     2401 aattaaaaca gataaacaat atatttccga tttctattac ttattaaaaa aattattaca
     2461 ttaaaaatta tttataaaat taaaaattgt tattatcata ttataaacct ccaactttta
     2521 ttattagtgg acctgatggg tgatgctccc actacctctt gtatgccata caagcgctct
     2581 actgatgagc tacaggccca tattaaaata ttttacacat atatatttat atatcataat
     2641 ttaccatatt tttaatacat tttcaagacc taatcctaaa atattataat ctattgaaaa
     2701 actatacaaa atatgatagt atatatgata gaatagtttt atcaaaataa taaataaagc
     2761 gtcgacgatt tattactata tataacttaa taaaaatata agtaattagt gttagtaatt
     2821 agtacttaga aataaacatt atatatatcc atacattatt ctttatattt ctaattacta
     2881 aatacaaaat actaaatact gaaaaattgg ttgcaaacac aatttttctg tgccgaggta
     2941 gctcagtggt ggagcgcttg cctgaagagc aaggcgtcgg gtgttcgatt caccccctcg
     3001 gcacatatta caaatatata taataatctt gtgttgttac aagaatatct tcatctttat
     3061 atggagaaca gaaaatttat ataacaataa acttaataaa tatattaata aatattaata
     3121 aatatgattc ctagattaaa ataccaaaaa ataaatatac ctactaataa tagtgctatt
     3181 gagggcactg tattagcaaa agataaaaaa gctttatatg ttgatgctag ccctttgggg
     3241 ttagccattg ttcgtggcta tgaatttaat gctgctagaa attttataaa agacctaaat
     3301 ataggagaca aagtaattat taaaatttta gaattagaca atgaattagg tcttatagaa
     3361 gcttcgctta aagaagccag cagagataaa atatgaaatt atcttactga tataaaaaga
     3421 aacaatgaaa caattgttgt acgcccaaca gaagctaatc gtggaggttt aattatagac
     3481 ttaaagggta tccaaggttt cttaccagca tctcaattat ctcctaaaca ttaccctgaa
     3541 gtagacggag gcgataaaga aactattctt gctaaactca aagatttagt aaatagagat
     3601 attgaagtca aaattttaga tttagacgag gaaactaata aacttatttt ttcagaaaaa
     3661 gaagttcaac atgaagaaga aaaacaaaaa ttagtaagca atttccaaat tggcgatgtt
     3721 gtggaatgca caataactaa aatagttccc tttggtatgt ttgttaaatt caacaatccc
     3781 ccaatcgatg gattaattca tatttcagaa atagattatc aattaattgc taatccagct
     3841 gatatataca aagaaggaga aaaaatacaa gctaaaattc aaagtattac taataacaaa
     3901 gtatctctgt ctataaaagc tttaaaaccc gatccttgaa aagatgccac agagatttat
     3961 aaagcaggaa aacaatatga agggaaaatt ctaaaaactg gtacgcttgg agtgcttgta
     4021 gaattcgaac caggtattta tggttttatg aaatataatg atgtagaaaa taaaaccaaa
     4081 attgaccaac cattagaaat tggagaaaaa agaaatttta ctatcaaaac aatagatcct
     4141 aatactagaa gaattgctct tactctttaa tttctcactc aaataaataa taataaagaa
     4201 ataacacatt tataataatt agtaaaaaat caaaaaaccc cgtcttactg aagtaaggcg
     4261 gggtaatttt ttaaaaaaac tactttactt tataagttct tttaatgctt tagcaggtct
     4321 aaatttagct acttttttcg cagcaatatt aattttttct cctgttttag gattaatacc
     4381 gactctagca gcttttgatt ttacggtaaa tataccaaaa ccactaattg ccgtgtcttc
     4441 gccctttttt aaagctttag ctatagaatc aaaaatacca ttaatgatat cttcggcttc
     4501 tttcttagtc atttgagttt tagcatggac caattcaact aaatcttctt ttttcatatt
     4561 actattagtt taaaaattgt tttttaaatg tagtaattac caagccagcg accttttact
     4621 acttagtaac cctaatttta agtatatgta aattatagca tttttatata ttttttgcaa
     4681 gtacctacat atattaacaa catattagta tattgggcaa tctaatattc aaaaacttgg
     4741 tttttgaaat atatctgata tatcggaata taaaagtaag aagtatagac taatacccct
     4801 tatactcttc
//
LOCUS       DAOTJA010000048         4746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_15672_length_4746_cov_4.564059, whole genome shotgun sequence.
ACCESSION   DAOTJA010000048 DAOTJA010000000
VERSION     DAOTJA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15672_length_4746_cov_4.564059"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="P5241_03470"
     CDS             <1..233
                     /locus_tag="P5241_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32564.1"
                     /translation="ILIAFLKSIKIILTSEVGVIVFSNLFSNDEISLLSSCCTCGCGC
                     YCNPAGAEAKGREGGSKGVSGTAAAKFSSVSS"
     gene            331..2073
                     /locus_tag="P5241_03475"
     CDS             331..2073
                     /locus_tag="P5241_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV32565.1"
                     /translation="MLKIICKEKKGRIYFVIKILLSFYSVIPKVVFLVFPGLIINELV
                     QDKNINNLLFYVLMIITIPIFDKLFLKIINTYLQKIKLDSLLKFGKKFDYHIMMMDYE
                     VFENPDIQDLIYRANATFTNSISFIDKTCEMLSAFLSLLVIFTILASLNIIIVLVVIV
                     LVFVNAYITKKNNNIQFENEKKLTKFNRFLSQIGSIPKMFIYAKEVRLFDMKDYLTNI
                     VINKGAEANNIRLDNVKKALNAQLYFSLTNFLQQGIIYAYLIWQVLEGNLSIGNMTIY
                     LSATIQFASLFSNFVQSYLNIAKDSLKIEEYKKLISIPLKQYELGHKIPIISERSIIE
                     FKNVSFKYPGSNILVLKNINLVLKLDEKICLVGTNGSGKTTLIKLLMRLYLPTEGEIL
                     LDGVNINEFEYEAYQKLFSPVFQDSRLYTVSIKENIVLNNNVNYDKLHKVCNQVMLLK
                     KIQSLPNGFDTPVFKLYDESGFEPSGGEFQRIAIARALYHDAPIYLLDEPTAALDPII
                     ENEIYRQFSNIISKKFAIMITHRLSIVKLTNEIILMDNGEILGFDSHSVLYEKNQFYK
                     NMYDVQAKYYQEHK"
     gene            2793..3134
                     /locus_tag="P5241_03480"
     CDS             2793..3134
                     /locus_tag="P5241_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PriCT-2 domain-containing protein"
                     /protein_id="HRV32566.1"
                     /translation="MDNILNALERINVSELTYTEWFQVGAALKHEGYDLGVWDNWSKN
                     DSRYKAGECERKWATMTGSSNPITGETIIQMTMKSKSMYMDWDNVIEYDGVVKIMVKW
                     IKYLFLIVITL"
     gene            3471..4742
                     /locus_tag="P5241_03485"
     CDS             3471..4742
                     /locus_tag="P5241_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV32567.1"
                     /translation="MKLIESYNVELKEKFTKKMLKTISAYSNYHDGYIYIGVNNNGEI
                     IGLPNIVQEKLNIENMINTTIKPTPFFEMNIIKIEQKEILEILVYKGENGPYYYDNVA
                     YMRNDTSTIPLDGPNLTRLVLASKNTSYDQLKVSNNDLSFNYLEEKIRSILNIQKINI
                     DILTTLGLYKNNTFNNGALLLSDKSDISQSYIDIARFKLDANIFLDRRRFENRSILEY
                     FDQVMRYFQEQYPPYQIIDGAQRITKEQIPLVAFRETLSNAIIHRDYLMNTGIQISMF
                     DNYIEFVSPGGLPEGMDEEQYYRGLTSLPRNPIIANVFFRLNLIEQFGTGIKRIVDSY
                     KQYRMKPYFEIRKSQIKVILPITDFDYSRLEKKEAILSYLRANPKSSRQKIEDAVKLD
                     KNTLIRRLNEFEKKGVVSRIGNGPSTVYVCN"
BASE COUNT         1857 a          498 c          767 g         1624 t
ORIGIN      
        1 ttatacttat cgcattcctg aagagtatca agattatatt aacatcggaa gtaggtgtaa
       61 ttgtcttctc gaatttgttt agtaatgatg aaatttcttt acttagttct tgttgtactt
      121 gtggatgtgg ttgctattgt aatcctgcag gagcagaagc aaaaggtaga gaaggaggtt
      181 caaaaggagt gtctggcaca gctgcagcta aattctcttc agtatcaagt taatattata
      241 tgagttaaaa taaaaagttt cgaaagagag gagataaaat tgaaaaataa aagtcaaaga
      301 ttaattaatg tgataaaaaa ttacatatat atgctaaaaa taatatgtaa agagaaaaaa
      361 ggacgtattt attttgttat taaaatttta ctatcatttt atagtgtgat tccaaaagtt
      421 gtatttttgg tttttcctgg tttaattatt aatgaattag ttcaagataa aaatattaat
      481 aatttattat tttatgtttt gatgatcatc accatcccta tttttgataa attattttta
      541 aaaataatta atacatattt acaaaaaata aaactggatt cattgttaaa attcggaaag
      601 aaatttgatt atcatattat gatgatggat tatgaagtat ttgaaaatcc tgatatacaa
      661 gatttgattt atagagcgaa tgctactttt actaattcta taagttttat tgataaaaca
      721 tgtgaaatgt tatctgcctt tctttcgttg cttgttattt ttactatatt agcatcttta
      781 aatattataa ttgttcttgt tgtaatagtg ctcgtttttg taaatgcata tataactaaa
      841 aaaaataata atattcaatt tgaaaatgaa aaaaaattaa ctaagtttaa tagatttttg
      901 agtcaaatag gttcaattcc taaaatgttt atatatgcta aagaagttag attgtttgac
      961 atgaaggatt atctaactaa tatagttatt aacaaaggag cagaagcaaa caatattcga
     1021 ttagacaatg ttaaaaaagc tcttaatgct cagttatatt tttctttaac taattttcta
     1081 cagcaaggta ttatatatgc atatttaatt tggcaagtgc tagaaggtaa tttatctata
     1141 ggtaatatga ctatttattt atctgcaaca attcaatttg ctagtttatt tagcaatttt
     1201 gttcaatctt atttaaatat tgctaaagat tctttaaaaa ttgaagaata taaaaaatta
     1261 atttcaattc cattaaaaca atatgaattg gggcacaaaa tacctattat tagtgagcga
     1321 tcgataatag agtttaaaaa tgtttctttt aaatatccag gaagtaatat tttagtacta
     1381 aagaatatta atttagtatt gaaattggat gaaaaaattt gtttagttgg tacaaatggc
     1441 tctggtaaga caactcttat aaaattattg atgagactat atttaccaac tgaaggcgaa
     1501 atattactag atggggtgaa tattaatgaa tttgagtatg aagcttatca aaaattattt
     1561 tcacctgtat ttcaagactc tagattatat actgtttcca taaaagaaaa tattgtttta
     1621 aataataatg ttaattatga taaactgcat aaggtttgca atcaagtaat gcttctaaaa
     1681 aaaatacaat ctttacctaa tggttttgat actccggtgt ttaagttata cgatgaaagc
     1741 ggatttgaac cttctggtgg ggaatttcaa agaatagcaa ttgctcgtgc tttatatcat
     1801 gatgccccta tttacttatt agatgagcca acagcagcgt tagatccaat tatcgaaaat
     1861 gaaatttatc gacaatttag taatataatt agtaaaaagt ttgcaataat gattacacat
     1921 agattgtcta ttgttaaatt aacaaatgag ataatattaa tggataacgg tgaaatatta
     1981 ggttttgact ctcattctgt tttatatgaa aaaaatcaat tttataaaaa tatgtacgat
     2041 gttcaagcga agtattacca agaacataaa taataataaa attataaata atgaatgtaa
     2101 ttgttgcaaa tagtcgcaca ttttaggtag gataaggaaa aagcaacaaa ttgctttttc
     2161 gacttcttgc gacaactggg atatccagat ttcgcttttt gtctgattgt aaaaatagtt
     2221 aaaggagtta actgttataa gtcaatatta aattaagcaa taaagactaa gttgtttgta
     2281 ccaaaggggt tgaaggggaa ttgatgacat taaaaaaatc cacagttaca gtaaactgtc
     2341 caaattgcgg agtgactttg cttaagattg agcatgtaat acacagcttg aactaaaatg
     2401 ttctggttgt aaaaagttga ttttgattaa agtacaagaa gatggtacta tctccacaaa
     2461 gctaaagaat aaaccaagaa ctacaaagga gagtagttga ttttttgaga attacaattt
     2521 aataactatc acgttcaaat tttttgattg aattataaaa aaatagagaa caaaaaaagc
     2581 aaaggcaaaa ataaaagaaa aaggcgaata taaaaaaata gataatagag ttttgtaaaa
     2641 tagagaaagc caagaaaaga ataattaaag accgtagcta cgtaaatagc tatgatgaaa
     2701 caaagtatat caatgatgta gataagtttt acgattatga tgaagcaata acaatttcag
     2761 aagacgatct tccatttcaa ggaatagtaa gtatggataa tattctaaac gcactagaaa
     2821 gaattaacgt ttcagaactt acttatactg aatggtttca agttggtgct gcattaaaac
     2881 acgaaggtta tgatcttggc gtttgggata attggagtaa gaacgattct agatataaag
     2941 ctggtgaatg tgaaaggaaa tgggctacca tgacaggtag ctcaaatcct attactggtg
     3001 aaacaattat tcaaatgacc atgaaaagta aatcaatgta tatggattgg gataatgtaa
     3061 ttgagtatga cggtgttgta aaaataatgg tgaagtggat aaaatattta ttcctgatag
     3121 tcataaccct ataattctta aagatgaatg gaatcaaatt caatatgaaa tgtctaagag
     3181 aagataatat tgtatataca atacaaaagt aaattatact ttttaggaga tatgttaagt
     3241 atctcctttt ttttatgtga tggaagtaag taaagtaatc gaaaggtgtg tatttttgta
     3301 atagctttat ttagtctgtt ttttatttgt aataattata cgatatgaaa ataaatcatt
     3361 tcaatttgag acgatacaaa acactatcgt tgcaaaacga gacgataaac aactttatag
     3421 ttgcaaaatg aaacaatttg tgttataata gttgcaaagg tggtgggtat atgaaattaa
     3481 ttgaatcata caatgtagag ttaaaagaaa agtttactaa aaaaatgttg aagacgattt
     3541 cagcatacag taattatcat gatggttaca tatatattgg tgttaataat aatggtgaaa
     3601 ttattgggtt acctaatatt gttcaagaaa aattaaatat tgaaaatatg attaatacaa
     3661 ctattaaacc gactcctttt tttgaaatga acattataaa aattgaacaa aaggagattt
     3721 tagaaatttt agtatataaa ggagaaaatg gaccatatta ttatgataat gttgcttata
     3781 tgagaaatga tacatcaaca attccattag atggtcctaa tttaacaaga cttgtgttag
     3841 catcaaaaaa cacatcatat gatcagctta aagtttccaa taatgattta agttttaatt
     3901 atttagaaga aaagattcgt tcaattctca atattcaaaa aattaatata gatattttaa
     3961 ctactttagg tctatataaa aataacactt ttaataatgg tgctttactt ttatcagata
     4021 aaagtgatat cagtcagtca tatatagata tagctaggtt taaattagat gcaaatattt
     4081 ttttagatag aagaagattc gaaaatcgat ctattttaga atattttgat caagtaatga
     4141 gatattttca agaacaatat ccaccatatc aaattataga tggtgcacaa agaataacta
     4201 aagaacaaat acctttagta gcttttcgag aaactctatc aaatgctata attcatagag
     4261 attatttgat gaatacagga atacaaattt caatgtttga taactatata gagtttgtat
     4321 cgcctggtgg attacctgaa ggtatggatg aagaacaata ttatagggga ctaacttcat
     4381 tacctagaaa tcctattatt gccaatgttt tttttagatt gaatttaatt gaacaatttg
     4441 gaactggtat taaaagaatt gttgatagtt acaaacaata tagaatgaaa ccatattttg
     4501 agattagaaa atcgcagata aaggttatat taccaattac cgattttgac tattcaagac
     4561 tagaaaaaaa ggaagctatt ctatcatatt tgagagctaa tccaaaatca tctagacaaa
     4621 agatagaaga tgctgtgaaa ttggataaaa atacgcttat aagaaggctt aatgaatttg
     4681 agaaaaaagg tgtcgtatca agaataggga atggtccaag cacagtgtat gtttgtaatt
     4741 gattat
//
LOCUS       DAOTJA010000049         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_16333_length_4575_cov_3.553319, whole genome shotgun sequence.
ACCESSION   DAOTJA010000049 DAOTJA010000000
VERSION     DAOTJA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16333_length_4575_cov_3.553319"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1090
                     /locus_tag="P5241_03490"
     CDS             131..1090
                     /locus_tag="P5241_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HRV32568.1"
                     /translation="MKNIFIIILFFLISFWGCRKEDEPQAPIIDSQVKVLKTHNIKTA
                     GPQYFSFETSDTTSNSSSNWDISFEITQIYYQTDDTKTWMKINDPVIKTGKAELARLE
                     GQSFDDLKTLPSGIKFRKDSSASAYIGKTWLDANNNIKNNVYIINTTINNVTKYYALA
                     IKSFVYDPASHQITSIKFYFKEITSGATVDTVNSSNPYTSNYYYSLQNKAKATDETNC
                     DFYFKGSELWLGLYSKVLVLENKTIDQVTSVPTGNFVTDKMESYVTMSWYEYNSSTHL
                     LTPKKETYIAKLLSGKYAAFKIVSYYGDDGKSGSFTINWKWIQ"
     gene            complement(1202..2173)
                     /locus_tag="P5241_03495"
     CDS             complement(1202..2173)
                     /locus_tag="P5241_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV32569.1"
                     /translation="MKKYFTINKNTKYLKNKNGIFMHSHNHLSDIQTTKRRMFIALLL
                     NIIIAISQIIGGVFSGSLSLISDSLHNLSDAVSLFLSYIAIKLKSKNHSYKHTFGFKR
                     AEIIVAFVNSSLLVGVAIYLFYEAIKRFIYPEVIKPDIMGIVAFIGLLGNVIGTILLS
                     KDSKTSINIKSAYLHLLSDTISSVAIVAGAISIYYFEVYWVDPLLTILIGIYILKESY
                     SILINSLHILMEGAPQNLSIEEIAKEVLKTEDVIDIHHIHIWSVGEKDIHIEAHLNVN
                     NMMISETDKIHKSIEKILLEKFGIKHVTLQLECEKCKGIGLIKDSVS"
     gene            2366..3910
                     /locus_tag="P5241_03500"
     CDS             2366..3910
                     /locus_tag="P5241_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRV32570.1"
                     /translation="MLKVINNAKGNPISTIKIPSGTHPIVINNLEPSTEYFLKFYNIY
                     NNGFDSIKLYTQSNYPSTINVYFNKKVDTTIAKIEKAKGEIDFAQKLINRIDSAKYSV
                     DLCVYSFSGSVGDAIADALINAKNRGVKIRFIIEEDNKDRAPVRKLINAGVPVLTDKC
                     GSNDGTGLQHNKFFIFDNRDNSSELDDLIISGSWNPTEPGTFDDFQNIVEIQNKSLAS
                     AYTTEFEEMWGSNSDNYDVTKAKFGKNKTDNTPHLFIIDGINVELYFSPTDKTLNRLI
                     DIIKTARNSINFSEMTFTNDDAANAIKLANSENKVTVRGIMNKEDAETATGSKYTFFN
                     SSPKWCTVLKSNYPTGIFHHKYMIIDGENINSNNAWLITGSYNITNSAEYYNNENLLF
                     FKSLRLADIYLQEFVQRFKECGGIYTNINNTLYIPETFSLEQNYPNPFNSTTKIKFSI
                     KSEKFVNLKIYDVLGNEIENPIKQDLRPGNYEIVWNASNLPSGVYYYTLKYGNELSTK
                     KMILIK"
     gene            complement(3926..>4575)
                     /locus_tag="P5241_03505"
     CDS             complement(3926..>4575)
                     /locus_tag="P5241_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRV32571.1"
                     /translation="FIDEIGDIPIQTQIKLLRFLQFNEIERVGGNQKITLDVRIIGAT
                     NRNLEDLIKKGLFREDFYYRINVVTINIPPLRERRKDIPLLVDHFIKKYASINNKGDI
                     SISKEAMDILIKYNYPGNVRELENIIERCVVLSRDNLITSNQLPINLKNSDSENIYDN
                     ELGEGETLEMRLEKIEKDFVMRALEKNNWNQTKAAEFLGIPERNLRYRLKKWGIK"
BASE COUNT         1750 a          681 c          580 g         1564 t
ORIGIN      
        1 acagtggaat taataattta acaaataaat atgatattca atggggtcca ttaccaggca
       61 gagagtggtt tattggattg caatacaact ttaaataaca taaatgaaca atataaggaa
      121 taatacttaa atgaaaaaca ttttcatcat tattttattt tttctaatat ccttctgggg
      181 ttgcagaaag gaagatgagc cacaagcacc aattattgac tcacaggtaa aggttttaaa
      241 gactcataat ataaaaactg caggaccaca atatttttca ttcgaaacat cagatactac
      301 atctaactct tcatcaaatt gggatatttc atttgagatt acacaaattt attatcaaac
      361 cgatgatacc aaaacatgga tgaaaattaa tgatcctgtg ataaaaaccg gaaaggcaga
      421 acttgcaaga ctagaaggcc aatcattcga tgatttaaaa acattaccct caggtataaa
      481 atttagaaaa gattcatccg cttcagcata tattggtaaa acatggttag atgctaataa
      541 taatattaaa aacaatgtat atataattaa taccaccatt aataatgtta caaaatatta
      601 cgcacttgct ataaagagtt ttgtatatga tcctgcatca caccaaatta catcaataaa
      661 attctatttc aaagaaatta caagtggtgc aacagttgat acagttaaca gttcaaatcc
      721 atatacttct aactattatt attcgctaca aaataaagcc aaagctacag atgaaacaaa
      781 ctgcgacttt tattttaaag gctcagaatt atggcttggt ttatactcaa aagttttagt
      841 tttagagaac aaaactattg atcaggtaac aagtgtacca acaggcaatt ttgtaacaga
      901 taaaatggaa tcgtatgtta ctatgtcctg gtatgaatat aattcatcaa cacatctatt
      961 aacacctaaa aaggaaacct atatagcaaa acttttaagt ggcaaatatg ctgctttcaa
     1021 aattgtaagc tattatggtg atgatggtaa atcaggatca tttacaatca attggaagtg
     1081 gatacaataa attcaaataa aaacatctaa agctgtcata gtatacaata tgacagcttt
     1141 ttttttaata atactcttca ggtaaaacaa atataaaaaa agaaatatta gccttactac
     1201 actaacttac actatccttt atcaagccaa ttcccttaca tttttcacat tctaattgta
     1261 atgttacatg ttttataccg aacttttcca ataatatttt ttctatagat ttatgaatct
     1321 tatctgtttc acttatcatc atattgttta catttaaatg agcttcaata tgaatatctt
     1381 tttctccaac actccaaata tgtatatgat gaatatcaat tacatcctca gttttcaata
     1441 cttctttagc aatttcttca attgataaat tctgtggtgc accttccatt aaaatatgta
     1501 acgaatttat aagtattgag taactctctt ttaaaatata tataccaatc aaaattgtca
     1561 gcaaaggatc aacccaataa acttcaaaat aatatataga aatagcccca gccacaatag
     1621 ccactgaaga tattgtatct gatagtaaat gcaagtatgc agatttaatg tttatactgg
     1681 tttttgaatc tttactcaga agtattgttc cgataacatt acctaataaa cctataaaag
     1741 ctactatacc cattatatca ggttttatta cttcagggta aataaatctt ttgatagctt
     1801 cataaaaaag ataaatagca acaccaacca ataatgagga atttacaaaa gctactataa
     1861 tctcggctct tttaaatcca aaagtatgtt tgtaagagtg gtttttcgac tttaatttta
     1921 ttgcgatata acttaaaaat aaagacactg catcactaag attatgtaat gaatcagata
     1981 ttaaagataa gctaccagaa aaaacacccc ctataatctg ggatatagca attataatgt
     2041 ttagtagtaa ggcgataaac attcttctct ttgttgtttg tatatcggat aaatgattat
     2101 gtgagtgcat aaaaatacca tttttatttt tcaaatattt tgtattttta tttatagtaa
     2161 aatatttttt caaaaaaaag atattttatt agttgcaata gaaaacaaat acaaacaaat
     2221 attaaaatag aaaattatga gaaagatctt tgtattatta attttaatat cataccatac
     2281 attttcacaa ttaaatttta atataacaaa tctaaatatt gataaataca aaatatcatt
     2341 ctgttataat tcagataggg atttattgct aaaagtaatt aataatgcta aaggtaaccc
     2401 tatttcaacc attaaaattc cttctggcac tcatccaata gtaattaata atttggaacc
     2461 atccacagaa tactttttaa aattttacaa catatacaat aatggatttg atagtataaa
     2521 attatatact caatcaaatt acccttctac aataaatgta tattttaata aaaaagttga
     2581 tactaccatc gcaaagattg aaaaagctaa aggagaaatt gactttgcac aaaaattaat
     2641 caatagaatt gattccgcaa aatattctgt tgatctttgt gtttatagtt ttagtggaag
     2701 tgtaggagat gcaatagccg atgccctgat taatgctaaa aatagaggtg taaaaataag
     2761 atttataatt gaagaagata acaaagatag agcacctgta agaaaactta taaatgctgg
     2821 cgtgcctgta ctaacagata aatgtggctc aaatgatgga actggtttac agcacaacaa
     2881 gttttttatc tttgataatc gtgataattc ttctgaatta gatgacttga taatctcagg
     2941 ttcgtggaat cctactgagc ccggcacatt tgatgatttt cagaatattg tagaaattca
     3001 aaacaaatca ttggcaagtg cttatactac agaatttgaa gaaatgtggg gctcaaactc
     3061 cgataattat gatgtcacaa aagctaaatt tgggaaaaat aaaaccgaca acactccaca
     3121 tttatttatt attgatggaa taaatgtaga attgtatttt tcaccaaccg ataaaacatt
     3181 aaatagatta attgatatta tcaagactgc tagaaatagt ataaactttt ctgaaatgac
     3241 ctttacaaat gatgatgctg ctaatgctat taaacttgct aattccgaaa acaaagttac
     3301 agtaagagga ataatgaaca aagaagatgc tgagactgct acaggctcaa aatacacttt
     3361 tttcaactct tcacctaaat ggtgtacagt attaaaatcg aactatccaa caggtatttt
     3421 ccatcataag tatatgatta ttgacggtga aaatattaat tcaaataatg catggttgat
     3481 tacaggttca tataatataa ctaacagtgc agaatattat aataatgaaa atctactttt
     3541 ttttaaaagt ttaagacttg ctgatattta tcttcaggag tttgtccaaa gattcaagga
     3601 gtgtggaggg atatatacaa atataaataa caccttatat attccagaaa ctttttcatt
     3661 agaacaaaac taccctaatc catttaattc aaccacaaaa attaaattct caattaaatc
     3721 agaaaaattt gtaaatttaa aaatatatga tgttttagga aatgaaatag aaaatccaat
     3781 taaacaagat ttaagacctg gcaattatga aatagtatgg aacgctagta acttaccaag
     3841 tggtgtatat tattatactt taaaatatgg aaatgaatta tcaacaaaaa aaatgatatt
     3901 aataaaatga gtatcagcta attaattatt ttatacccca tttttttaat ctatatctta
     3961 aatttctttc tggtatacca aggaattctg ccgcttttgt ttgattccaa ttgttttttt
     4021 ccaaagctct catcacaaaa tctttttcaa ttttctcaag tcgcatttct aaagtttcac
     4081 cttctcccaa ctcattatca tatatatttt cgctatcaga gtttttcaaa ttaattggca
     4141 attggtttga agtaataaga ttatctcttg ataaaacaac acatctttca attatgtttt
     4201 ccagttcacg aacattaccg ggataattgt atttaatcaa aatatccata gcttcttttg
     4261 aaatacttat atcccctttg ttattaattg atgcatattt tttaataaaa tgatcaacta
     4321 ataaaggaat atcttttcgt ctttctctta aaggtggtat attaattgtc acaacattaa
     4381 ttctataata aaagtcttct ctaaaaagtc cctttttaat taaatcttcg agatttctat
     4441 ttgtagctcc aataatcctt acatctaatg taattttttg atttccaccg actctttcaa
     4501 tttcattaaa ttgtaaaaac cgtaataact ttatttgtgt ttgtattggt atatctccaa
     4561 tttcatcaat aaata
//
LOCUS       DAOTJA010000050         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_16724_length_4483_cov_3.699413, whole genome shotgun sequence.
ACCESSION   DAOTJA010000050 DAOTJA010000000
VERSION     DAOTJA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16724_length_4483_cov_3.699413"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="P5241_03510"
     CDS             <1..1150
                     /locus_tag="P5241_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRV32572.1"
                     /translation="DVDWYRYISSKIVKKINYKYGNAYETVGYKTNGDSDDWLYGDNK
                     SKNKIFAFSPELGSSTDYFWATIDRIEPIAEEAMCINNFICLYSGAYIDSVCIKGENF
                     ICSNDTSIVAIQLMNGGLREVDSCIIQISTTDTSLILINNKLMVNHIKAFNVSDTVFY
                     RISFKKNTPFGYTAKYKLMLNYNGLPYQKDLSITKINDKPVNYESFDITQENNKILLK
                     WTTNYEMRHNGFFIDKKSNNQNYLQIGYVPSKGELGGLYTFLDFPKEAGYYTYRIRTY
                     DNNEPKYTPEKNIYFKLYPYRYSLFQNYPNPSNPETTISFYLPYRTKLELSIYNVLGK
                     KIKTIVDNYYEEGLHNVKVNLSDLPSQVYFYELKTNDYREVKKLILVK"
     gene            1231..2352
                     /locus_tag="P5241_03515"
     CDS             1231..2352
                     /locus_tag="P5241_03515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRV32573.1"
                     /translation="MPKAKPEELGFSSKRLSRIDVVVKKAIDEKKIPGAVVLVARKGK
                     VVYKKAFGYASLEPEKKILTDDMIFDLASVSKPIGTATAVMMLVEEGKLRLTDEVRKY
                     IPDFAPYIDKDGKMEHAKIYHLLTHTSGLPPYTNADTVKMLYGELAGDSLMNYIARLS
                     KNNPPGAKFDYSCLGFITLANIVKIVSGKSLKDFDKEYIFDPLKLKHIGYVPDEEMKK
                     KCVPTEKTDGVILCGVVHDPLARLQHGISGNAGLFSNVDDLAVMVQMYLNGGEYNGVR
                     ILSPLTVKLMTTIYEPVKKSGRGLGWDIASDYMGQRGDIFPLGSYGHTGFTGTSIIVD
                     PFSQTQLIILSNRVHPDGKGEIMQLRKDVANIVASSLVK"
     gene            2720..3781
                     /locus_tag="P5241_03520"
     CDS             2720..3781
                     /locus_tag="P5241_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV32574.1"
                     /translation="MSVDHFQMGNDYFHMGKYIEAIKAYEEALIIDPEGADIYYNIGV
                     SYFSLEDYENSIFYFLKAIEKRNDFLDAYNNMSICYSRLGRTDKVIETLKQILKLNPY
                     YIPAYYNLGIIYYSMKNYEEAAIAYKQVVDLKPDFPEAHNNLGGTYKVLGRIEDSIRE
                     LTIAIKLKHDYAEAYNNLGNIYYDIGKYDEAIENYSIAIHYKPKLPEAYNNMGNAYKS
                     LGKFQEAEVALKKAIELNPLLPEAYNNLSIVMSKLGKLEETFKYLDKALELNPDYAEA
                     LNNYGLALKNNKEYEKALDKFLKAISISPNLAQAHFNLAETYLLTGKKNEAIKEFNIL
                     KELNIDLANKLDVIMKNSK"
     gene            3889..>4483
                     /locus_tag="P5241_03525"
     CDS             3889..>4483
                     /locus_tag="P5241_03525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRV32575.1"
                     /translation="MRYYNSNEYYSPRGFGGFSLFPPVIKMLLITNIGLYILQIFLSS
                     YTIDDIPLEHYVIKYFALIPLGYGFKIWQLVTYMFLHADFTHVFFNMFALWMFGVELE
                     QVLGSRKFTFFYFACGIGGGLAHLLLGPSFTHPAPTIGASGAVYGVLLAFGVLFPDRL
                     IFLYFLIPIKAKYFVAMYILLEIFAVGSNDLVAHLAHL"
BASE COUNT         1626 a          546 c          739 g         1572 t
ORIGIN      
        1 agatgttgat tggtatagat atatttcttc gaaaattgtg aaaaaaatta attacaaata
       61 cggtaacgcc tatgaaaccg taggttacaa aacgaatggg gattctgatg attggttata
      121 tggtgataat aaaagtaaga ataaaatatt tgctttttct cctgaacttg gttcatctac
      181 agattacttt tgggctacta ttgatagaat tgaaccgata gcagaagaag ctatgtgtat
      241 taataatttt atttgtttat actccggggc atatattgat tctgtttgta ttaaaggaga
      301 aaactttatt tgtagtaatg atacatctat tgtagctata caattaatga atggtggctt
      361 gagagaagtc gatagttgta ttatccagat ttcaactact gatacaagtt tgatactcat
      421 aaataataag ttgatggtta atcacataaa agcttttaat gttagcgata cagtttttta
      481 tagaattagc tttaagaaaa acacaccctt tggatatact gctaaataca agcttatgtt
      541 aaattataat ggattacctt atcaaaaaga tctttcaata acgaaaatta atgacaaacc
      601 tgttaattat gaatctttcg atataacaca ggaaaacaat aaaatattat taaagtggac
      661 tacaaactat gaaatgaggc acaatggttt tttcattgat aaaaaaagca ataaccagaa
      721 ttacttacaa ataggttacg ttccatcaaa gggagaatta ggtggattat atacatttct
      781 tgattttcct aaagaagctg gttattatac ttacagaata agaacgtatg ataataacga
      841 accaaaatat accccagaga aaaacatata ttttaaactt tatccttatc gttatagttt
      901 atttcagaat taccccaatc catctaatcc tgaaaccact ataagttttt atttacctta
      961 tagaacaaaa cttgaacttt ccatatataa tgtattaggt aaaaagatta aaacaattgt
     1021 tgataattat tatgaagaag gtctacacaa tgttaaggta aatttgtcag atttgccatc
     1081 gcaagtttat ttttatgaat taaagacaaa tgattataga gaagttaaaa aattaatatt
     1141 agttaaataa ttttattagg tgatataatg aaaaaactgt tatttttttt aatcgttatt
     1201 tttattagtc aaaattcatt agctcaaccg ttgccaaaag caaaacctga ggaactaggt
     1261 ttttcttcaa aaagattatc aagaattgat gttgttgtta aaaaagctat tgatgagaaa
     1321 aagatacctg gagctgttgt tctggtagca agaaaaggaa aagttgtata taaaaaagct
     1381 tttggctatg caagccttga accagagaaa aaaattctta ctgatgatat gattttcgat
     1441 ctcgcttccg tatctaagcc aattggaacc gcaacagctg taatgatgtt ggttgaagaa
     1501 ggaaaacttc gcttaactga tgaggtaaga aaatatatac cggattttgc tccttatata
     1561 gataaagatg gtaaaatgga gcatgcaaaa atttatcatc ttcttacaca cacttccggg
     1621 ttgcctccat atactaatgc tgatactgta aaaatgttgt atggtgagtt agcaggtgat
     1681 agtttgatga attatattgc aaggttaagt aaaaacaacc ctcctggagc taaatttgat
     1741 tatagttgcc ttggatttat cacattagca aatattgtca aaattgtatc aggtaagtca
     1801 ttgaaagatt ttgataagga atacattttt gatccattaa agttaaaaca tattgggtat
     1861 gttcctgatg aagaaatgaa gaaaaaatgt gtacctactg aaaaaactga tggggtaata
     1921 ttatgtggtg ttgtacatga ccctttagca agattgcaac atggtatttc tggtaatgcg
     1981 ggtttgtttt caaatgttga tgatcttgca gttatggttc aaatgtattt aaatggtggt
     2041 gagtataacg gagtaaggat tttatctcca cttactgtta aattgatgac aacaatatat
     2101 gaacctgtaa aaaaatctgg tagaggactt ggttgggata tagcttcaga ttatatgggt
     2161 caaagaggag atattttccc gcttggttct tatggccata ctggttttac aggaacttct
     2221 attatagttg atccattttc tcaaactcaa ttaattatac tctctaatag agttcaccct
     2281 gatggtaaag gggaaatcat gcaattaaga aaagatgttg caaatattgt agcttcatca
     2341 ttagttaaat aaaatttttt gttccgggaa cctaaaattc agacataaat tttgggttcc
     2401 cagaaataat aagttgctta tttatgattt tataattaaa ttttaataac acatcttgat
     2461 ataatttaca tatttaagat agttatttaa ataatctata aaattgttta ttctaactaa
     2521 aaaaataata ataaactaaa acattactta ttcgaaaatt tagagaaaaa agaaaatatt
     2581 cttaaatttt attaaaagtt aacttagaaa aaatagtaag ttacttttaa gttcatagta
     2641 ttattttttt cttgattttt aaaatgaaaa ctacttaatt aaaaatagga gatctttcaa
     2701 caaataatga gagttggcta tgagtgttga tcattttcaa atgggcaatg actattttca
     2761 catgggtaaa tatattgaag ctattaaagc ttatgaggag gctcttataa tagatcctga
     2821 aggagcagat atttactata atataggagt ttcatatttc agtttagagg attatgaaaa
     2881 ttctatattc tattttttaa aagctattga aaaaagaaat gattttttgg atgcttataa
     2941 taacatgagc atttgttata gtcgcctggg tagaacagat aaagtaattg aaacattaaa
     3001 acaaatttta aaattaaatc catattatat tcccgcatat tataatttag gtattatata
     3061 ttatagtatg aaaaattatg aagaagccgc aatcgcttat aaacaggttg tagatctcaa
     3121 acctgatttt cccgaagcac ataataactt aggaggcact tataaagtac ttggtagaat
     3181 tgaggattca attagagaat taaccatagc aattaaacta aaacatgatt acgctgaggc
     3241 ttataataat cttggtaata tttactatga tatcggtaaa tatgatgaag ccattgagaa
     3301 ttattcaata gcaattcact ataaacctaa acttcccgaa gcatataaca atatgggaaa
     3361 cgcatataaa tctttaggca agtttcaaga agctgaagtt gctttaaaaa aagctataga
     3421 attaaatcct cttttgcctg aagcatataa taatcttagc attgtaatga gtaaattagg
     3481 taaattagag gaaacattta agtatctgga taaagcgtta gaattgaatc cagattatgc
     3541 agaagcgttg aataattatg gattggcttt gaaaaataac aaggagtatg aaaaagcttt
     3601 agataaattt ttgaaagcaa tttcaatcag cccgaattta gctcaagcac attttaacct
     3661 tgcagaaaca tatttgttaa ctggtaagaa aaatgaagca attaaggaat ttaatatttt
     3721 aaaagaatta aatattgacc ttgcaaataa gttagatgtt attatgaaaa attctaaata
     3781 agatccaatt tgtaaattta tagtgtaaaa acccaatctt taatagcttg actcaaataa
     3841 tataaaatag tatattttaa agtgtttcta taaacaaaga gtatttaaat gcgttattac
     3901 aatagtaatg aatattatag tcctagaggt tttgggggtt tcagtttatt ccctcctgtg
     3961 ataaaaatgt tattaattac caatataggt ttgtatattt tacaaatttt tttaagtagt
     4021 tatacaatag atgatattcc acttgaacat tatgtaatta agtattttgc ccttatacca
     4081 ttaggttacg gatttaaaat ctggcaactt gtaacatata tgtttttgca tgcagatttt
     4141 acccatgttt tctttaatat gtttgctttg tggatgtttg gggttgagtt agaacaggta
     4201 ttaggttcaa gaaaatttac atttttttat tttgcttgtg gaatcggtgg aggattagct
     4261 catttacttt taggaccatc gtttactcat ccagctccaa ctattggagc ctctggtgct
     4321 gtttacggtg ttttgttggc ttttggtgtt ttattccctg atagactaat ttttttatat
     4381 ttccttatcc caataaaagc gaaatatttt gttgcaatgt atattttatt agaaattttt
     4441 gcagtaggca gtaatgatct ggtcgcacat ttagctcatt tag
//
LOCUS       DAOTJA010000051         4448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_16881_length_4448_cov_2.534031, whole genome shotgun sequence.
ACCESSION   DAOTJA010000051 DAOTJA010000000
VERSION     DAOTJA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_4448_cov_2.534031"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="P5241_03530"
     CDS             complement(<1..1825)
                     /locus_tag="P5241_03530"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRV32576.1"
                     /translation="MNTQKRIYYIFSFLILISFKVYSQTYSWYWQNPRPQGNPLFKIT
                     AFDTSKAVAVGTVGTIMSTSDGGFSWQVTHKLGGSDYWLWSTHYYNPSLGWAVGGNGN
                     IFKTENGGFSWIKQYSGITSTLQSVFFVDLFNGWAVGWNGAILKTTNSGETWFAQDSK
                     TQADLYSVYFLNKDTGFCVGIRGTVETALVGEFLKTTNGGETWQQVKLGIDNRLWGIT
                     FQHGVGWIYGWGGLLLKSTDRGGSWSPKNPSLTESIYSVSFPTENIGYAAGEGGLLLK
                     TTNKGETWFQLKRVTEDTIYSISFVNERIGWFVGENGIIYKTRDGGNSWAQQSSGLTK
                     WITSTDFIGTTGWAVGEKGTIIKTTDNGVTWKNYSTRNNLYNEVKFPNLSIGFIAGNG
                     LVLKSTDGGETWRDVSPSNDNYSSVHFIDGYTGYVCGNNKFYKTTNGGFTWNRIIISN
                     NDSYSKVYFLNQNIGWVVGKNGSIYKTTNGGSTWIRQNTPTTKTILFIKFINERLGWA
                     SGEDGLIIKTTNGGNSWTSGSSSFGDEISKIYINDSLNLWAVGKWGSLFKSIDGGNNW
                     SKIQSVTSNFLSTLFFPDVSNGLVFGKGGTILRFREGVQKPV"
     gene            complement(1850..2344)
                     /locus_tag="P5241_03535"
     CDS             complement(1850..2344)
                     /locus_tag="P5241_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32577.1"
                     /translation="MKIIKFVTVIFIYLLVSLINVFSQEQISKKTLKEVRYLINDSTG
                     SYLIKETILIDGSKDYSFIPKSFLYDTNEVQTVLPDSVKDWFYKVKNKNGSYSYSQSN
                     EKDSLSYLFKTTEQEEEVKDKKNKTAWWIIGSSTVAGGIIAYIFLKYRKGLDELPIQP
                     DPPK"
     gene            complement(2413..3165)
                     /locus_tag="P5241_03540"
     CDS             complement(2413..3165)
                     /locus_tag="P5241_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRV32578.1"
                     /translation="MISKLINIKLYIIFLTFATLSSTSAQNKKISIHTNIDSAFVFID
                     NQFIGYTPINDFIISKDTINLLITDENVLNWSSQKINENIIVNKDTSIFIYFKHKVYV
                     NTLPDNAEIIFNDKSIGKSPLFILYDNTTINKIEIKKENYHSLVLKTDSTFPKTISLK
                     LQELNTDQDKNLKKIKSNNNLNEYLIIGGIISGIISGYSKMRADHYEAEYYNNKTNTN
                     KEKVYLYDTISNISLIIFECSLISISYLLLKD"
     gene            complement(3140..4273)
                     /locus_tag="P5241_03545"
     CDS             complement(3140..4273)
                     /locus_tag="P5241_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRV32579.1"
                     /translation="MKKLYKILLILILFILIGCSSQIIIERALKINQDDWITLGGSNS
                     RTNITNEVLKLPFTKLWDYQGPAGISSNQPLLADSILFVGFMNGELYAINLKNGKRIG
                     RIKVDAAIHGAPCLSKNVLYIPIAQHKYSLRAYNINEGYLIWKKEIEGIESSLLLSDQ
                     YLYAVTENGTIYCIKPIDGKTIWDYKLPKKSYSSLAADSSKLYVGCDDGFLYCINKNN
                     GKLLWKFYTSLPIRSTPLVGKEGNIYFGSSDSNFYSVSADSGFMNWKFNSNSPIISGS
                     SSNDSKIYFGNLSGYFYCLDKLTGNLRWSFKTKGNIISSPILTKEHVAFSSYDKNIYV
                     LNKNSGELFWSIELDGRPKTTPIFWNNYMIICYEDYDIEAYKY"
BASE COUNT         1526 a          695 c          555 g         1672 t
ORIGIN      
        1 ttacaggttt ttgaacaccc tcacgaaacc ttaatattgt tccacctttt ccaaaaacaa
       61 gaccatttga aacatctggg aaaaagagtg tacttaagaa attactagtg acagattgaa
      121 ttttgctcca attattacca ccatcaatag atttaaataa tgaaccccat ttacctacag
      181 cccataagtt taatgagtca ttaatatata ttttggaaat ctcatctcca aagcttgagg
      241 atcctgaagt ccaactattt cctccatttg ttgttttaat tatcaatcca tcttcaccag
      301 atgcccaacc taatctttca tttatgaatt taataaataa aattgttttt gtagttggag
      361 tattttgtct aatccatgtt gaacctccat ttgtggtttt ataaattgaa ccatttttgc
      421 caactaccca tccaatattt tgatttaaaa agtatacttt actgtatgaa tcattattag
      481 atattataat ccgattccat gtaaaacctc catttgtggt tttgtaaaat ttgttattac
      541 cacatacata accagtatag ccatctataa aatgcacact actgtaatta tcatttgatg
      601 gtgaaacatc tctccatgtt tctccaccat cagtgctttt taatactaat ccattgccag
      661 caatgaaacc aatagataaa tttggaaact taacttcatt ataaagatta tttcttgtac
      721 tgtaattttt ccatgttaca ccattatcag ttgtttttat tatggtacct ttttcaccaa
      781 cagcccaacc agtagtgcca ataaaatctg tagaggtaat ccattttgtt agtccagagc
      841 tttgttgggc ccatgaattt ccaccatcac gggttttata gataatgcca ttttcaccta
      901 caaaccaacc aattctttca ttaacaaagc taatcgaata aattgtatct tcagttactc
      961 gctttaactg aaaccaagtt tctcctttat ttgttgtttt caataataaa ccaccttcac
     1021 cagcagcata tccaatattt tctgtaggga aagatacaga gtaaatagat tcagttagtg
     1081 atgggttttt tggagaccaa gagccaccac gatctgttga ttttaaaagt aaaccgcccc
     1141 aaccataaat ccatcctaca ccatgctgaa atgtaatacc ccataaccta ttatcaatgc
     1201 caagttttac ttgttgccat gtttcaccac catttgttgt ttttaaaaat tcacctacca
     1261 atgcagtctc aactgttcct cttataccta cacaaaaacc agtatcttta tttagaaagt
     1321 aaaccgagta caaatctgct tgtgtttttg aatcttgtgc aaaccaggtt tctccactat
     1381 tcgttgtttt caaaattgca ccattccaac caacagccca gccattaaat agatcaacaa
     1441 agaaaactga ttgtaaagtt gaagtaatac cagaatattg ttttatccaa ctaaagccac
     1501 cattttctgt tttaaaaata tttccatttc ctccaacagc ccaacctaat gaagggttat
     1561 agtagtgagt tgaccataac caataatcac tcccacctaa tttatgagtt acctgccagg
     1621 aaaaaccacc atcagatgta gacataattg taccaactgt accaacagct acagcttttg
     1681 aagtatcgaa tgcagtgatc ttaaaaagag gattcccttg aggtcttgga ttttgccaat
     1741 accagctata agtttgtgaa taaaccttaa aagaaattag aataagaaag ctgaatatgt
     1801 aatatatcct cttttgggta ttcatatatt ttaaaaattg tataattttt tattttggtg
     1861 gatcaggttg tataggtaat tcatctaatc cttttcgata ctttaaaaat atgtatgcaa
     1921 ttattcctcc tgcaacagta ctagagccta taatccacca tgcggtttta ttttttttat
     1981 ctttaacttc ttcttcttgc tcagtagttt taaataaata tgataaagag tctttttcgt
     2041 tggattgtga gtaggaataa ctaccatttt tatttttaac tttataaaac caatctttta
     2101 ctgagtcagg taatacagtt tgaacttcat ttgtatcata aagaaaagat tttggtataa
     2161 agctataatc ttttgaccca tcgatcaaaa tggtttcttt aattaaatat gatccagttg
     2221 aatcatttat taagtagcgt acttctttta atgttttttt actaatttgc tcttgagaaa
     2281 aaacattaat taaggaaacc aatagataaa taaatattac tgtaacaaat tttataattt
     2341 tcattagttt aatggttcaa tttattaatt attatcggta aaagagcaat ttacttgaca
     2401 aaaattatgt ttttaatctt ttaataataa ataacttatt gatattaatg aacactcaaa
     2461 tataattaaa ctaatatttg aaatagtatc ataaaggtat actttttctt tattagtatt
     2521 tgttttattg ttgtaatatt cagcttcata atggtctgct ctcatttttg aataacctga
     2581 aattattcct gaaataattc caccaattat taaatattca ttcaggttat tattagattt
     2641 gattttttta aggtttttat cctgatcagt atttaattct tgtaatttta atgatattgt
     2701 ttttgggaat gttgaatcag tttttaaaac taatgaatga taattttctt tttttatttc
     2761 aattttattt attgttgtgt tatcatatag aataaaaagg ggggatttac cgatagattt
     2821 atcattaaaa attatttctg cattatcagg tagagtattt acataaacct tatgtttaaa
     2881 ataaataaaa attgacgtgt ctttgtttac aattatgttt tcatttattt tttgactgga
     2941 ccagtttaaa acattttcat ctgtaatcaa gagattgatt gtgtctttag aaattataaa
     3001 gtcattaatc ggtgtgtatc caataaattg gttgtcaata aaaacaaatg cactatcaat
     3061 atttgtatga atgcttattt ttttgttttg agctgaagta gatgataaag tagcaaatgt
     3121 taaaaatatt atatatagtt taatatttat aagcttcgat atcataatct tcatagcaaa
     3181 ttatcatgta attattccaa aaaattggag ttgtttttgg tctgccatcg agttcaatac
     3241 tccaaaaaag ttcgcctgaa tttttattca aaacataaat atttttatcg taagaagaaa
     3301 aagcaacatg ttcttttgtt agaatagggg aagatattat attacctttt gttttaaatg
     3361 accaccttaa attacctgtt aatttgtcta aacagtaaaa atatcctgat aaattaccga
     3421 aataaatttt actatcattt gatgatgaac cactaataat tggtgaattt gagttaaatt
     3481 tccagttcat aaagccacta tcagctgaaa ctgaataaaa atttgaatca cttgatccaa
     3541 aataaatatt tccttctttt ccaactaaag gagtgcttct aataggtaaa gaagtataaa
     3601 atttccacaa taatttaccg ttatttttat ttatacaata taaaaaacca tcatcacaac
     3661 caacatataa ttttgaacta tcagctgcaa gagaagagta tgatttttta ggtaatttat
     3721 aatcccaaat tgttttgcca tctatgggtt ttatacaata aatagtgcca ttttcagtta
     3781 cagcatataa atattggtcg cttaataata atgaactttc aataccttct atttcttttt
     3841 tccagattaa atatccttca ttgatattat aagctcttaa agaatactta tgttgagcaa
     3901 taggaatgta taaaacattt tttgataaac aaggagcacc atgtatagca gcatctacct
     3961 ttattcttcc aattctttta ccatttttta aatttatggc atataattct ccattcatga
     4021 aacctacaaa tagaattgaa tcagctaata atggttgatt tgagcttatt cctgctggtc
     4081 cttgataatc ccataatttt gtaaatggca attttagaac ttcatttgta atatttgttc
     4141 ttgaattaga acctcctaat gttatccaat catcttgatt gattttgagt gctctctcaa
     4201 ttattatttg agaagagcaa ccaataagaa taaataatat caatattaat aatattttat
     4261 ataatttttt catgttatat ttaatataaa ttatatatta ataaaagcaa ggtaaagttt
     4321 tttcagtaaa actaaagaaa taaataattt gagaatgaaa tttaaattta aaatgggatt
     4381 tcatttaaaa ttcgcttaaa aaggctaaaa tactaaattt aacattgact ttttgaaaaa
     4441 atttcaat
//
LOCUS       DAOTJA010000052         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_17060_length_4402_cov_5.685760, whole genome shotgun sequence.
ACCESSION   DAOTJA010000052 DAOTJA010000000
VERSION     DAOTJA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17060_length_4402_cov_5.685760"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="P5241_03550"
     CDS             complement(<1..244)
                     /locus_tag="P5241_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32580.1"
                     /translation="MKEKLVGEITHYFGKIDVAIVKLHDILKEGDVIHIKGKDTDFTQ
                     TVSSMQIEHKNVKTAKKGATIGMKVNNKIKEGDEIYL"
     gene            complement(441..584)
                     /locus_tag="P5241_03555"
     CDS             complement(441..584)
                     /locus_tag="P5241_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32581.1"
                     /translation="MAGQSFREGPSAKSAVNEGSVGEEALPTINAEDEESPFGDEDDE
                     IPF"
     gene            complement(654..908)
                     /gene="ssb"
                     /locus_tag="P5241_03560"
                     /pseudo
     CDS             complement(654..908)
                     /gene="ssb"
                     /locus_tag="P5241_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(1009..1476)
                     /locus_tag="P5241_03565"
     CDS             complement(1009..1476)
                     /locus_tag="P5241_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRV32582.1"
                     /translation="MIQQIFEDIQNKLQNKGFVIISSLAPEKKLLANNINKEKEAYLA
                     SIAFGIGEGDIENIKDIFAFNDNILRFLITKKEMRPEKPKIKRSRSGYKSKFAFRDDN
                     FNKEKISEDNNVEYVAQEQQIKNGSAEQNSCEPKIEQEIDLNELDKKLDELLN"
     gene            complement(1564..2589)
                     /gene="recA"
                     /locus_tag="P5241_03570"
     CDS             complement(1564..2589)
                     /gene="recA"
                     /locus_tag="P5241_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRV32583.1"
                     /translation="MANKKTKISDNINNIEDILDSTVREIKERFGEGSIMKLGDARHV
                     DVDVIPTGSPSLNIALGVGGIPRGRIIEIYGAESSGKTTLALHIVAEIQKKGEVAAYI
                     DAEHALDPEYAKKIGVNIKNLLISQPDNGEQALEIVETLVKSGAVSLIVVDSVAALTP
                     LAEIEGEMSDQQIGLQARLMSKALRKLTALSAKVNTTIIFINQTRMQIQTMAYGNPET
                     TPGGKALKFYTSVRIELKRIAQIKKGEDVVGNRIRAKVVKNKVAAPFKIAEFDIMYNE
                     GISYSKDLLGLGIKYNIVKRTGTTYFFNDIKLGVGLDAASEAIKTDSKLAKELFNQIQ
                     EKSVANI"
     gene            complement(2745..3041)
                     /locus_tag="P5241_03575"
     CDS             complement(2745..3041)
                     /locus_tag="P5241_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32584.1"
                     /translation="MGAKIFSRPAITISGNMIDLNTLMRETNKIKEQNMANIQGFVDD
                     KIQEDIYYVASSRGKNYYPSTCSAWKSLSPDNLIKFNTEEEAKTAGYTLSKQCQ"
     gene            3201..3473
                     /locus_tag="P5241_03580"
     CDS             3201..3473
                     /locus_tag="P5241_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRV32585.1"
                     /translation="MSKTKGGSSTKLGRDSESKRLGIKIHGGAKANIGNVIIKQRGTK
                     WYAGRNVRQGADDTLYATKTGIVQFTEKYKTDFTGKKRKIKLVNIV"
     gene            complement(3456..3926)
                     /gene="rplI"
                     /locus_tag="P5241_03585"
     CDS             complement(3456..3926)
                     /gene="rplI"
                     /locus_tag="P5241_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRV32586.1"
                     /translation="MKIILTQNVPNLGMKNEIKEVQDGYAKNYLFKNKLAVLATESRI
                     KQVQKQQALQEKKLLEQQNVYKEMAKQIEAIDFIEVELNVAKEGGESFEAVKAETIQD
                     ILKNKYQIVLDENVKIQFDNKIKEKGDYEISIKFPFGIISPLKLKAIEKLNNIH"
     gene            complement(3953..>4402)
                     /locus_tag="P5241_03590"
     CDS             complement(3953..>4402)
                     /locus_tag="P5241_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRV32587.1"
                     /translation="IGNYFFADNTLLLIEDYEDKQFNKEYKTFRDGELKDIPIVVLIN
                     EGTASAAEILAGALRDNRGVKLVGENSFGKGSVQELVDLSGGNQLKITVAHWLTPNGT
                     DISKKGIKPDIVVKLEQEEDNGSYSTITLENDSQLKRAYDEIISSVK"
BASE COUNT         1403 a          795 c          511 g         1693 t
ORIGIN      
        1 ctaaataaat ctcatcacct tccttaattt tattattaac tttcatacca attgttgctc
       61 cttttttggc tgttttaaca ttcttatgct ctatttgcat agaagaaaca gtttgggtaa
      121 aatctgtatc cttaccttta atatgaataa catcaccttc tttcaaaata tcgtgaagtt
      181 ttacaatggc cacatctatt ttaccaaaat aatgagtaat ttctccaact aatttttcct
      241 tcatagttta tttattaaat taaattttat ttttcgacca ttgaacaata atgatactaa
      301 ataataattt ttaatttggt aaataataag aattagaaat taaaaattgg aaagaataaa
      361 attgctttgt ttataataca aataaataag tttattgtat gggtattttt gagtcaatca
      421 gcattgtcaa atcacattta ttaaaacggg atctcatcat cttcatctcc aaatggactt
      481 tcttcatctt cagcgtttat agtaggcaaa gcctcttccc cgaccgaacc ttcattcaca
      541 gcactcttgg ctgatggtcc ttctctaaat gattgccctg ccaaattggg accaaattct
      601 aaattatcta caataatttc agtgcgtgat tgttttttgt tttctttatc aattcaagtt
      661 cgtgttgaaa ttcttccttc taccaaaatt aatttaccct ttgttaaata tggcgataaa
      721 gcctcagctc ttttccccca tattactaaa ttatgaaaat cgggtttttc ttgtttttga
      781 ccattcttat ctactcatgt cctattagta gccaaaccaa gtgcaagcat agattgtcca
      841 cttggcaatg tttttaattc tccatccctt gttaaatttc ctatcaatat aactttgtta
      901 taaaacataa taaatattta acagctgaat tataatatta caccaatata ctaaatagtt
      961 atacgaccaa attattaatc taaacacaac acatcaaaaa ataattaact aatttaataa
     1021 ctcatcaagt tttttatcaa gttcgtttag atcaatctcc tgttcgattt ttggttcaca
     1081 actattctgc tcagccgacc cattttttat ttgttgttcc tgagcaacat attctacgtt
     1141 attatcttcg ctgattttct ctttattaaa attatcatcg cgaaaagcaa attttgattt
     1201 gtatccagat cgtgatcttt tgattttggg cttctcaggg cgcatctctt ttttagttat
     1261 caaaaatctt aaaatattat cattaaaggc aaaaatatct tttatatttt ctatatctcc
     1321 ttcacctatt ccaaacgcaa tagacgccaa ataagcttct ttttctttat ttatattgtt
     1381 tgccaataat ttcttttcag gcgccaaact agaaataata acaaaccctt tgttttgtaa
     1441 tttattttga atatcttcaa atatctgttg aatcatagat tcatcactat tttgtttaat
     1501 tcaaaatact aaatcatatt ctactatatt attcataaat tgtatttaaa tattttttaa
     1561 ttattaaata tttgccacgc tcttttcttg aatttgatta aagagctctt tggctaattt
     1621 tgaatctgtt tttatagctt cagaagctgc atccaaccct acacccaact taatatcatt
     1681 aaaaaaataa gttgtaccag ttcttttaac tatattatat tttataccca gccccaacaa
     1741 atcttttgaa taagaaatcc cctcgttgta cataatatca aactcagcaa ttttaaacgg
     1801 agcagctact ttatttttta ctaccttagc acgtattcta ttacctacaa catcttcgcc
     1861 ttttttaatt tgagcaattc gttttaattc aattcttaca gaagtataaa attttaacgc
     1921 cttgccacca ggtgttgttt ctggatttcc ataagccata gtttgaattt gcatacgtgt
     1981 ctgattaata aaaataattg ttgtattcac ttttgcactc aaagctgtta atttacgtaa
     2041 cgccttactc atcaatcttg cttgcaaacc aatttgttga tcagacattt caccttctat
     2101 ttctgccaaa ggtgtcagag cagcaactga atctactaca atcaaagata ctgccccaga
     2161 ctttaccaat gtttcgacaa tttccaaagc ctgttcgcca ttatctggct gagaaattaa
     2221 aagatttttt atatttacac ctattttttt agcatattca ggatccaaag catgctcagc
     2281 atcaatatat gcagcaactt cgcccttttt ttgaatttct gctactatat gcaaagccaa
     2341 agtagtttta ccagaagatt cagctccata tatttctata attctaccac gaggtatacc
     2401 ccctacaccc aaagcaatat tcaaagaggg cgagcctgtg gggataacat caacatcaac
     2461 atgtcgagca tcaccaagct tcataataga tccttctcca aaacgctctt taatctctcg
     2521 cactgtgcta tctaaaatat cttcgatatt gtttatatta tctgatattt tagttttttt
     2581 gttagccata atatattttt gttgtgttta aaagattttt aattctatta atattttagc
     2641 atataattat ataaaattca agtaggtatt gatatggatt tatcataaag attttttatt
     2701 tggatatttt gatttttaat ttatcctggt atacaataaa ctacttattg gcattgttta
     2761 gataatgtat atccggctgt cttggcttct tcttctgtat taaatttgat taaattatct
     2821 gggctcaaag atttccaagc gctacatgta cttggataat aatttttccc ccgagagcta
     2881 gcaacataat aaatatcttc ttgaatttta tcatctacaa acccctgtat atttgccata
     2941 ttttgttctt taattttatt tgtctctctc attaaagtat tcaaatcaat catattacca
     3001 gatatagtaa tagctggacg agaaaatatt ttagccccca tacaaaatcc tacaccaaaa
     3061 cataatataa acaataaaat tgaaattatg tgttttagca aaaaattgct aaatatcttt
     3121 acaaaagaag aaaattgtgt tagtatattc tttgccatca aaatatatta aatttttatt
     3181 aaaattttaa atgattaaaa atgtctaaaa caaaaggagg tagttctaca aaattaggac
     3241 gtgattccga atccaaaaga ttaggtatca aaattcatgg aggtgcaaaa gcaaatattg
     3301 gtaatgttat tattaaacaa cgaggtacta aatggtatgc tggtagaaat gtacgccaag
     3361 gagctgatga cactctctat gctaccaaaa ctggtattgt acaatttacc gaaaaataca
     3421 aaacagattt tactggcaaa aaaagaaaaa taaaattagt gaatattgtt taatttttct
     3481 attgccttga gctttaaagg agatatgatc ccaaaaggaa acttaatgga gatttcataa
     3541 tctccttttt cttttatttt attatcaaat tgaattttaa cattttcatc caaaacaatc
     3601 tgatatttat tttttaatat atcttgaata gtttctgcct ttaccgcttc aaagctttct
     3661 ccgccttctt tggcaacatt aagctctact tcaataaaat caattgcctc tatttgtttt
     3721 gccatttctt tatacacatt ttgctgttcc aataatttct tttcttgtaa agcttgctgc
     3781 ttttggactt gttttatacg actttctgtg gcaagcactg ccagtttatt tttgaataaa
     3841 taatttttag cataaccatc ttgtacttcc ttgatttcat ttttcattcc taaatttgga
     3901 acattttgag ttaatattat tttcatatca atatattatt tttatttatt gtttatttaa
     3961 cacttgatat aatctcgtca taagctctct tgagctgact atcattttct aatgttatag
     4021 tactataaga accattatct tcttcttgtt ctaactttac tactatatct ggcttaatac
     4081 cttttttaga aatatctgtg ccatttggtg ttaaccagtg agcaacagta atttttaact
     4141 gattacctcc tgacaaatcc accaattctt gtacgctacc tttaccaaaa ctattttctc
     4201 ctactaattt aactccccta ttgtcacgca aagcgccagc caaaatttcc gccgccgaag
     4261 cagtgccttc atttatcaaa acaacaatag gtatatcttt taattctccg tctctaaatg
     4321 ttttgtactc tttattaaac tgcttgtctt cataatcttc aatcagtaat aaagtattat
     4381 cagcaaaaaa ataattacca at
//
LOCUS       DAOTJA010000053         4196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_18014_length_4196_cov_3.232070, whole genome shotgun sequence.
ACCESSION   DAOTJA010000053 DAOTJA010000000
VERSION     DAOTJA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18014_length_4196_cov_3.232070"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(608..1258)
                     /locus_tag="P5241_03595"
     CDS             complement(608..1258)
                     /locus_tag="P5241_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32588.1"
                     /translation="MRKIIFYLLLFFLALKTFSQDKDYFSTEFDANYYYYFVGNNNSN
                     NFNYGFSILISKYINKVKVSSGINYSTKSYNTQGDPFWLIEKREYNLDYLNFPIITNI
                     EIFSHRKFSSSVLTGFTFNQIVDYKIKSFYLNGETLIEDNLLNDKKLGLTFLLGTTFS
                     KSIGNKWLLNLSPFINYKLIPDHNDQRPDYKNIPDDRVFLGFKIGIEYLFKTSDKY"
     gene            complement(1260..2774)
                     /locus_tag="P5241_03600"
     CDS             complement(1260..2774)
                     /locus_tag="P5241_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRV32589.1"
                     /translation="MKKAIFIFLMICSSIIYAQTLCEGQKVIATENFNQCNTDPWVLV
                     FEDNFNGNSLDLSKWSYGWRILYCNNEQQYYTSGDNIEVSNGTLKLIAKEETIYARAV
                     DWLPDDEILYCDDENRGQNARYFYYTSGNIQTIRKFSYGKFEARIKIPKGKGFWPAFW
                     MFGGDPVYNEIDVFEFWNENDIWGNYDPSKLSKVHHMTIHYDFDGDGNKSQCATKYSG
                     VDFSQNFHVFSLIWDENKIEWYVDSSLKRTDYRFYTILGQPTGCTINAWGQYIMNKIY
                     TRDPMHIILNLAIQSGSNSPNSSTPFPSQMEVDWVRYYQRNPCQDVNITDASQYPLDN
                     QFYNVIVGEDVNINCNYTIPYDQQLDIVSKNGIILGPGFSAELGSTFNARIEPTVCGS
                     SLKSSRIENVQDTLVTNKDFETQITDNTFSNEDIWIFPNPNEGSFIVDFGSKDYRNYN
                     IAVTDIEGKLIKSFKELNSSILNIDLSGNTKGVYVLYLLNNQDKNIITHKIILQ"
     gene            complement(2838..3410)
                     /locus_tag="P5241_03605"
     CDS             complement(2838..3410)
                     /locus_tag="P5241_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32590.1"
                     /translation="MKKFTLISLALFLSIFAFSQNVYKNEVILTVGKFYSPYRYFKYY
                     NGFESIKFEYSHQFSSNMGVSFVQEYGRMFDENTNSRAFVIPSNFCIYYNMLQNKDLK
                     LRISAGTGLEQIYIKSLYEFYPVERNFYGIPVRLEIGFFYKLNERLNLTLNAVTTYCS
                     LFSNKNSLFPSPYYYNTLFFSSNIGLSYYF"
     gene            complement(3533..4108)
                     /locus_tag="P5241_03610"
     CDS             complement(3533..4108)
                     /locus_tag="P5241_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32591.1"
                     /translation="MKNKSVIIIFNIFLTLALLSCKKETIPPCGILMDVSSINCYIAT
                     EMTAINNFDNYYYYYSNPNNDTVLYSEYNIYLYFNGKMVSKGIENPKYSNYGELQYFI
                     NKIKNFNIKSDKIFKEGESLTNCFIKEDNCGTILTNYQPLGYSPIILSLEMAPDSEEY
                     YIFTIEITDIYDNVFTASTDSIFILKTNKKL"
BASE COUNT         1455 a          596 c          532 g         1613 t
ORIGIN      
        1 ctagctggta tcctttcatt agctttcatg ggattcactg gaataattta atagtctagg
       61 catttatatt gcgaatttac tcttattcaa tttttgggtt tttagtaata acttaattag
      121 ttgaagagga gacggggaga ggaggtgact gggtgacgag gagacgagga aagaatgtaa
      181 taggtaatag gcaataggca ataggtgata ggtgataggt ggtagagttg attagttgaa
      241 aggtttaagc taaatgttta aactattttt aaactatatt aatctatttt aaaacaattt
      301 taaactattc ttcattattt tcttttcgat accttttatt tcattaattt ttttcaatta
      361 aaataatgca atatgctgaa atagttttac atttacctat gctatctaat ttttcatttg
      421 ttttagcttt aatagataat ttgtcttttg aaatatttag aaaattgcaa agattttttt
      481 gaatcatttc aatataaggt ttttttcagg atgataatgc tatcgatatt agaaatatta
      541 taattgaaag gtttacaaat ttcaataatt tgtttgagca aatctataga atgttgtcaa
      601 ccgtaaatca atatttatcc gatgttttaa ataaatattc tattcctatt ttaaatccta
      661 aaaatactct gtcgtcaggt atatttttgt aatcaggtct ttgatcatta tggtcaggaa
      721 tcaacttata attaataaaa ggagacaaat ttaatagcca cttattccca attgattttg
      781 agaaagtagt accgagcaaa aaagttaagc ctaatttctt gtcatttaat aagttatctt
      841 caattaatgt ctctccgttc aaataaaatg atttaatttt ataatctaca atttgattaa
      901 atgtaaagcc agtaagaaca cttgaactga atttcctatg tgaaaatatt tcaatatttg
      961 taataattgg aaagtttaaa taatctaaat tatattctcg tttttcgatt aaccaaaatg
     1021 ggtctccttg agtattataa gatttagttg aatagtttat tccagaactc acttttacct
     1081 tattaatata ttttgagata agtatagaaa atccataatt aaaattatta gaattattgt
     1141 tcccaacaaa gtaataataa taatttgcat caaattctgt tgaaaagtaa tctttatctt
     1201 gactaaatgt ttttaatgcc aagaagaaca ataagaggta aaatattatt tttctcatat
     1261 tattgtagta ttattttgtg agtaattatg tttttatcct gattgtttaa caaatacaga
     1321 acataaacgc ctttagtatt accacttaaa tcaatattta atattgatga atttaactct
     1381 ttgaatgatt taatgagttt tccttctata tctgttacag caatattata gtttctataa
     1441 tctttagagc caaaatcaac tataaagcta ccttcattcg gatttggaaa tatccaaata
     1501 tcctcattcg aaaaagtatt atctgtaatt tgtgtttcaa aatccttatt agttacaaga
     1561 gtatcttgaa cattttcaat tcttgaactt tttaatgatg aaccacatac tgtgggttca
     1621 atccttgcgt taaatgttga gcctagctca gcgctaaatc caggtcccag aattatgccg
     1681 tttttagaaa ctatgtcaag ttgttgatca tatgggatag tgtagttgca attaatatta
     1741 acatcctcac caactattac attataaaac tgattatcca aaggatattg actggcatca
     1801 gttatattta catcttgaca tggattcctt tgatagtatc tcacccaatc aacctccatt
     1861 tgacttggaa aaggggtcga actgtttgga gaattactgc cactttgaat ggccaaattt
     1921 aatataatgt gcattgggtc ccttgtataa atcttattca tgatgtactg cccccaagca
     1981 tttattgtac aaccagttgg ttgtcctaaa attgtataaa atctataatc agttcttttt
     2041 aatgaactat caacatacca ctcaattttg ttttcatccc aaataagaga aaaaacatga
     2101 aaattttgtg aaaagtctac tcctgaatat ttcgttgcac attgagattt atttccgtcc
     2161 ccatcaaaat catagtgtat cgtcatatga tgaactttag acaatttaga aggatcataa
     2221 ttaccccaga tgtcattttc attccagaat tcaaaaacat caatttcatt atatacagga
     2281 tctcctccaa acatccagaa agctggccaa aacccctttc ctttagggat ttttattctg
     2341 gcctcaaatt ttccataaga aaatttcctt atagtttgaa tattgccgga tgtataataa
     2401 aaatatcgag cattttgacc tctattttca tcatcacaat acaatatttc atcatcaggc
     2461 aaccaatcca ctgctcttgc ataaatggtt tcctcttttg cgataagttt tagagttcca
     2521 ttactaacct caatattatc accactcgtg tagtactgtt gttcattatt acaataaaga
     2581 attctccatc cataagacca cttagataaa tctaaactat ttccattaaa attatcttca
     2641 aatacaagta cccaaggatc agtattacac tgattaaaat tctctgtagc tataactttt
     2701 tgaccttcac ataatgtttg ggcataaatt attgatgaac atatcattaa aaaaataaaa
     2761 attgcttttt tcattacttt taattttatt ttctaattgt ttgatttgtt tttgttgcta
     2821 cataattatg aaatttgcta aaaataataa cttaatccga tatttgatga aaaaaacaaa
     2881 gtattatagt aatatggact aggaaataaa ctatttttat tactaaacag ggagcaatat
     2941 gtagtaacag cattaagtgt taggtttagt ctttcgttta gtttataaaa aaaaccaatt
     3001 tctaatcgca ctgggatacc ataaaaattc ctctcaactg gataaaattc atacaaagat
     3061 ttaatataaa tttgttcaag tcctgttcct gctgatattc ttaattttaa atctttattt
     3121 tgaagcatgt tgtaataaat acaaaaattt gagggtatca caaatgctct actgtttgta
     3181 ttttcatcaa acattctacc atattcttgt acaaatgaaa cacccatatt ggaagaaaat
     3241 tgatgtgaat actcaaattt tatactctca aaaccattgt aatatttaaa atacctataa
     3301 ggagaataaa atttccctac tgtcaaaata acttcatttt tatatacatt ttgtgaaaat
     3361 gcaaatattg ataaaaataa agctaaactt attaatgtga atttcttcat tgtttaatta
     3421 ttttacattg ataattgtat ttataagttt tatcattata ggctgatcat aactaatgta
     3481 ttgcaaaaaa aataaaaaga ttaaaatatt aacaatattt ttattcataa tcctataatt
     3541 ttttattagt ttttaaaata aatattgaat ctgtactagc agtgaaaaca ttgtcataaa
     3601 tgtctgtaat ttctatcgta aagatataat attcctcact atctggagcc atttcgagcg
     3661 ataagataat tggtgaatac cctaatggtt gataatttgt caatatagta ccacaattat
     3721 cttctttaat aaaacaattt gttagacttt ctccttcctt aaatatttta tcagatttta
     3781 tattaaaatt tttaatttta ttgataaaat attgcagttc gccatagtta ctatatttag
     3841 gattttcaat accttttgat accattttac cattaaaata caagtatata ttatattctg
     3901 agtataaaac agtatcatta ttaggatttg aatagtagta atagtaatta tcaaaattat
     3961 ttattgctgt catctctgtt gctatataac aattaatgga actaacatcc attaatatac
     4021 cacaaggagg gattgtttcc tttttgcatg ataatagcgc taaagttaaa aaaatattaa
     4081 atattattat tactgattta ttcttcattg ctttatgttt ttaattttat tttctaattg
     4141 ttctatttgt ttttgctgct ctataacata aagcgtcagc tcttctacct ttttta
//
LOCUS       DAOTJA010000054         3935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_19415_length_3935_cov_3.244072, whole genome shotgun sequence.
ACCESSION   DAOTJA010000054 DAOTJA010000000
VERSION     DAOTJA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19415_length_3935_cov_3.244072"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1266
                     /locus_tag="P5241_03615"
     CDS             229..1266
                     /locus_tag="P5241_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRV32592.1"
                     /translation="MTSKEIVIKTLKFDNPERVPRHLWHLPWAKFNYPKELEEIQKRF
                     PDDITSPPNCYDKISEDIYNFYEPEGASHQPGEATDEWGCTFLNYNKGTIGEVKNPPV
                     KDWSQINNIKIPYDYLSIDVDKVNSFCKSTDKFVLAGLNGGNMPRPFEKLQFIRGSEN
                     LYIDLAEKPKELFDFLEKSHDFYKQIFEMWAKTDIDGLVFMDDWGSQNSLLISPKTWV
                     EIFKPLYKEYVEIGHKYNKFVFMHSDGYILDIYPHLIEIGVDAINSQIFCMGVKNLEK
                     YKGKISFWGEIDRQHLLPYGSLQDIENAVISIKNSLYYNGGVFAQCEFGPGAKPENVY
                     KVFETWEKIMR"
     gene            1263..2363
                     /locus_tag="P5241_03620"
     CDS             1263..2363
                     /locus_tag="P5241_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV32593.1"
                     /translation="MKKNNSFLNTYSRYIKYWSIDTNYIFLNHGSFGATPIEILKKQQ
                     IIRNQLENEPVKFFIRDIEEQLFISKKKLAKFINADVKDIAFIPNATYGVNSVLKSLT
                     FRKGDELLTTNHEYHSCKNTLDLVAKKNNSKVVVANIPFPPKSENEVIEALLKYVTKK
                     TRLLLIDHVTSPTGLVFPIEKITKEFESRGIDVLVDGAHAAGMVDLKINKFKPSYYTG
                     NCHKWLCTPKGSAFLYVREDKQKNIYPLITTYSDPQFEKGKWTIFQNRFYWLGTIDVS
                     PFICVGHTIDYLNKLYPNGLKGIIEHNHSLAIQARNVLSKALKIDLPIPDNMIGSLVS
                     LPITDTKRKNFLVFILMIFKISFFMIIRLKCL"
     gene            2689..3627
                     /locus_tag="P5241_03625"
     CDS             2689..3627
                     /locus_tag="P5241_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV32594.1"
                     /translation="MNKTKIILNLILLLYSYLLFSQNINYDSYSSLQNKCNNIFKNID
                     GCLGIVELKSNKIITTVNKKLLIENRFKPGSIVKIITAIAAINSGKVDILEEFNCTGT
                     ANFDNESYKCWLPKGHSKVNLIKAISLSCNLYFLNIASKLTIDEILSTFTEFHFDEKT
                     IFNIPGEATGLFRKNKIDIEKYYLSIGCSENLLVTPLQILQMITFIANKKLPNTKNID
                     LNNPKFDPIYKGLRNSVLFGTSKESNYLKIQPAGKTGTISEKYNIKNSAWFIGYAPYY
                     NPEIAIVIFIKNGRGATNAALIAKKVFKCYYELFHR"
     gene            3600..>3935
                     /locus_tag="P5241_03630"
     CDS             3600..>3935
                     /locus_tag="P5241_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32595.1"
                     /translation="MLLRIISSITLVIIFFTCNPQKNSEQEKQTYFKIAVLSLFKPQI
                     AEIVFPENIVIRTENNEKISVKSNKKISVELINSNLVLKVNNIKLTSNKFYFFTENFN
                     PYYNKFSINL"
BASE COUNT         1545 a          537 c          540 g         1313 t
ORIGIN      
        1 taatagaata tatgcagctc tgaataaatc gaaaaaatac ccttactata ttttaaataa
       61 aataattata tataatatat tgtcaatact aatttttatt accccttcca aaaaacatta
      121 aattaaaaca tttaaaatgt tttcaagcat agaataaaat tatttttatt agttgcactt
      181 ttaaaataaa tattgtactt ttactttcaa atttattgga gagggaaaat gacatcaaaa
      241 gaaatagtaa taaaaacatt aaaattcgat aatcctgaaa gagtacccag gcacttatgg
      301 catctaccct gggcaaaatt taattatcct aaagaactag aagaaataca aaaaagattt
      361 cctgacgata ttacatctcc acctaattgc tacgataaaa tttctgagga tatttataat
      421 ttttatgaac ctgaaggtgc atctcatcaa ccgggtgaag caactgatga atggggttgt
      481 acattcttaa attataacaa aggaacaatt ggagaagtaa aaaatccccc tgttaaggat
      541 tggtctcaaa taaataacat taaaatacct tatgattatt taagtattga tgtagataaa
      601 gtaaattcat tttgtaaaag cactgataaa tttgtgttag cgggattaaa tggtggcaat
      661 atgccccgac cctttgaaaa gttacaattt attcgtggtt ctgaaaacct gtatatagat
      721 cttgctgaaa aaccaaaaga actttttgat tttctggaaa aatcacatga cttctacaaa
      781 caaatatttg aaatgtgggc caaaacagat atcgatggat tggtatttat ggatgattgg
      841 ggaagtcaaa actctttatt aatatcaccc aaaacatggg ttgaaatttt taaaccttta
      901 tacaaagaat atgtagagat tggccataaa tacaataaat tcgtttttat gcactcagat
      961 ggttatattc ttgatatata tcctcattta atcgaaattg gtgttgatgc tataaattct
     1021 caaatctttt gtatgggtgt aaaaaatcta gagaaatata aaggaaaaat aagtttttgg
     1081 ggagaaattg accgccaaca tcttcttccc tatggttcat tacaagatat tgaaaatgct
     1141 gtaatatcta ttaaaaactc tctttattat aatggtggag tttttgctca atgtgaattt
     1201 ggtcctggtg ctaaacctga aaatgtatat aaagtttttg aaacgtggga aaaaataatg
     1261 cgatgaaaaa aaataattca tttcttaaca catattccag atatataaaa tattggagta
     1321 ttgatactaa ttatattttt ttaaatcacg gttctttcgg agcaactcca attgagatac
     1381 tcaaaaaaca gcaaattatc agaaatcaac ttgaaaatga gcctgtaaaa ttttttattc
     1441 gtgatataga agaacaacta tttattagca aaaagaaatt agctaagttc ataaatgcag
     1501 atgtaaagga tattgcattt ataccgaatg ctacatatgg ggtaaattct gttctaaaat
     1561 cattaacatt tagaaaagga gatgagttat taactacaaa tcatgaatat cactcctgta
     1621 aaaatacttt agatctggtt gctaaaaaaa ataattcaaa agttgttgtt gcgaatatcc
     1681 cttttcctcc aaaatcagaa aatgaagtaa ttgaagcttt attaaaatac gttactaaaa
     1741 agactcgctt gctattaatt gatcatgtaa caagtccaac tggtttagtc tttcctattg
     1801 aaaaaattac caaggagttt gaatcaagag gaatcgatgt tttagtagat ggagcccatg
     1861 ctgctggtat ggttgatttg aaaattaaca aattcaaacc ttcatattac actggtaact
     1921 gtcataaatg gttatgtaca cccaaaggta gtgctttttt atacgttcga gaagataaac
     1981 aaaagaacat ttacccacta attacaacat attctgatcc tcaatttgaa aaaggtaaat
     2041 ggactatttt ccagaatcgc ttttactggt tagggactat agatgtttct ccttttattt
     2101 gcgttggtca tacaattgat tatttgaata agctttaccc taacggcttg aaaggaataa
     2161 ttgaacataa tcatagttta gctattcaag caagaaatgt tttatctaaa gcattaaaaa
     2221 ttgatttgcc gataccagat aatatgattg gatcactagt ttcattacca ataacggata
     2281 caaaaagaaa aaatttccta gtttttatat tgatgatctt caaaataagc ttttttatga
     2341 ttataagatt gaagtgccta taatgttttt ccctaaccct cctaaaagag ttattagggt
     2401 atcagctcaa atatataatt caataaaaca gtatgaaatt ttaaaagata tacttataga
     2461 tattatatag aaattatata aaattctggt attcttaaat tttactcaat attttacgtt
     2521 gaaaattttt cattttttct ttaaattaat aattataatt ctatatcaat aataaaatat
     2581 ggaggtataa tgaataagag aaaaagaaga gttcttaaag ttcacagaaa gaaagaagct
     2641 aaaagaaaag caaaattaaa agcagcaaaa gcatctgctc aatcataatt gaataaaact
     2701 aaaatcatat taaatttaat cttactttta tatagctatc ttttattttc tcaaaatata
     2761 aattatgata gctatagctc tcttcaaaat aaatgcaata atatatttaa aaatattgac
     2821 ggttgtttag gtatagttga attaaaaagc aacaaaatca ttacaaccgt caataaaaaa
     2881 cttttaattg agaatagatt taaacctggc tcaattgtaa aaattattac agcaattgcg
     2941 gcaattaatt ctggtaaagt agatatcctt gaagaattta attgcacagg tactgctaac
     3001 tttgataatg aaagctacaa atgctggtta ccaaaagggc attctaaagt aaatttgata
     3061 aaagcaatat cactttcatg taatttgtat tttttaaata ttgcctcaaa acttacaatt
     3121 gatgaaatac tctccacttt cacagagttt cattttgatg aaaaaactat cttcaacatt
     3181 cctggtgaag ctactggatt attcagaaaa aataaaatcg atatagaaaa atattacctt
     3241 tcaattggtt gtagcgaaaa cttattggta acccctttac aaatactaca aatgataact
     3301 tttatagcaa ataaaaaact acctaacaca aaaaatattg atcttaataa tccaaagttt
     3361 gatccgatat ataaaggctt acgtaattct gttttatttg gaacatccaa agaaagtaac
     3421 tatttaaaaa ttcaaccagc tgggaaaaca ggcactattt cagagaaata taacataaaa
     3481 aattccgcat ggtttatagg atatgcacct tattacaatc cagagatagc tattgtaata
     3541 tttattaaaa atggacgtgg agctacaaat gcagctctaa tagcaaaaaa ggtttttaaa
     3601 tgttattacg aattatttca tcgataaccc ttgttattat tttttttacc tgcaatcctc
     3661 aaaaaaatag tgaacaagaa aaacagactt acttcaaaat tgcagtactt agtttattta
     3721 aaccacaaat tgcggaaatt gtttttcctg aaaacattgt tattagaaca gaaaataacg
     3781 aaaaaatatc tgtaaaaagt aacaaaaaaa tatccgtgga attaattaat tccaatttag
     3841 tcttaaaagt aaataacatt aaacttactt caaataaatt ttattttttt actgaaaatt
     3901 ttaatcctta ctacaataaa ttctcaataa atctt
//
LOCUS       DAOTJA010000055         3912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_19571_length_3912_cov_3.880477, whole genome shotgun sequence.
ACCESSION   DAOTJA010000055 DAOTJA010000000
VERSION     DAOTJA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19571_length_3912_cov_3.880477"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="P5241_03635"
     CDS             complement(<1..245)
                     /locus_tag="P5241_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRV32596.1"
                     /translation="MNKLERTLVLLKPDAVQRNLIGDIIGRFERVGLKIIAMKFLLPT
                     KEQAYKHYVKNPEEITALGQRSIDGKRKKGLEVNDDPT"
     gene            complement(278..607)
                     /locus_tag="P5241_03640"
                     /pseudo
     CDS             complement(278..607)
                     /locus_tag="P5241_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
     gene            complement(761..1495)
                     /locus_tag="P5241_03645"
     CDS             complement(761..1495)
                     /locus_tag="P5241_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32597.1"
                     /translation="MTGIVKERPANMINSKIITGHIEFEVKNVEILNQSQVHPFDIYQ
                     DGKDINEEIRLAHRYLDLKRPRMHNNLKKRALVFKLIRDYLYDKDFLEIETPYLTKST
                     PEGARDFLVPSRLGAGLFYALPQSPQQYKQMLMVSQFEKYFQIVRCFRDEDPRGDRQP
                     EFTQLDLEVSFYSQDQILELTENLVKNIVEQIYPEKHFTAFPFPRINYQDAIKQYNSD
                     KPDIRNNKEDINELGCVFIVQFPMFE"
     gene            complement(1505..1615)
                     /locus_tag="P5241_03650"
                     /pseudo
     CDS             complement(1505..1615)
                     /locus_tag="P5241_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
     gene            complement(1845..2675)
                     /locus_tag="P5241_03655"
     CDS             complement(1845..2675)
                     /locus_tag="P5241_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV32598.1"
                     /translation="MDKNYEKYKKVAIGGLVVINIIIYYAIFNYIYFKPKVLFLPVGE
                     GDSELIKTKAGNILIDAGPKQNVLVPLSQKTSLFDKTIDIALITHPDVDHFEGLLKIL
                     DYYKVRLVVLNNFSSQNGTYIELLLRLCKQKIPIVVGTTNTIIYLGQSDTFLKILYPD
                     IQNLQNIKADNDFSIISLLNLNNKKFLFTGDITAKLLDGVVDKFLKGQTIDVLKVPHH
                     GAKNTLSDNILNNILVKQAIIEVGKNSYGHPTDFIINLLRDFGISYKRTDLDGIIEFQ
                     "
     gene            complement(2722..3519)
                     /locus_tag="P5241_03660"
                     /pseudo
     CDS             complement(2722..3519)
                     /locus_tag="P5241_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            complement(3631..>3912)
                     /locus_tag="P5241_03665"
     CDS             complement(3631..>3912)
                     /locus_tag="P5241_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32599.1"
                     /translation="MRNPVEIEDKKYLAVKPIYQDQDNAKNLKDLILVSVPFYQDIHK
                     GDKILLMGKLQANTYNPFDVFLNNKLVYTIYNGTIQIVEPSLHKDFLFM"
BASE COUNT         1431 a          634 c          424 g         1423 t
ORIGIN      
        1 gttggatcat catttacttc taatcccttt tttcttttgc catcaataga tctttgaccg
       61 agagctgtta tttcttctgg atttttaaca taatgtttat acgcttgctc ttttgttggc
      121 aataaaaatt tcatagctat aattttcaaa ccaactcttt caaatcgacc aataatatca
      181 ccaatcaaat tacgttgaac cgcatctggt tttaataata ccaaagttct ttctaatttg
      241 ttcatactac aaatttaaaa tttattaatt ttattaatta ttgtttacta atttgtatat
      301 gtaattcttt taattgatct ggatcaactt cagcaggagc atgcatcatc aaatcgcgat
      361 tgtcacctgt tttaggaaac ggtattactt cacgaatact agattcgttt tgtaatatca
      421 ttaacaacct atctaaacca aaagcaatac ccccatgcca aggaactcca tattggaaca
      481 tttctatata atgagcaaat tgtctatgga aatcttcctc actattacct aataatttaa
      541 atacttcctg taaaatatta atatcagttt ccctaatcga tcccccggct atctcgtagc
      601 catttaacgc taaatcatat tgtcaagcct gcaaagtatt aggagaattt ctaataaggt
      661 taatcttctc gtcctggctt aaagaatcat caaatctaat ttttgtaaaa ggatggtgca
      721 cagctgtaaa atgagctttt tcattatatt gttgttcgtg tcattcaaac attggaaact
      781 gtacaataaa aacgcaacct aactcattaa tgtcttcttt gttattacgt atatctggtt
      841 tatcagaatt atattgttta attgcgtctt gatagttaat tctgggaaaa ggaaaagctg
      901 taaaatgttt ttctggataa atttgttcta caatgttttt tactaaattt tctgttaatt
      961 ctaatatttg atcttgtgaa taaaaactta cctccaaatc aagttgtgta aattccggct
     1021 gtctatcacc ccgaggatct tcgtccctga aacatcttac aatttgaaaa tatttctcaa
     1081 attgtgatac cattaacatt tgtttatatt gttggggtga ttgtggtaaa gcataaaaca
     1141 aaccagcacc taatcttgaa ggaaccaaaa aatctctcgc cccctcaggc gttgatttag
     1201 tcaaataagg agtttctatt tccaaaaaat ctttgtcata cagataatct ctaatcaact
     1261 taaaaaccaa agctcttttt ttcaaattat tatgcattcg tggcctcttc aaatcaagat
     1321 atctatgtgc taatcttatt tcttcattga tatctttacc gtcttgatat atatcaaaag
     1381 gatgaacttg cgattggttc aaaatttcta catttttgac ttcaaattca atatgccctg
     1441 taataatttt agaatttatc atatttgctg ggcgttcttt aactattcca gtcattttga
     1501 ctactcattc tggtcttaat ttatctgctt ctttataatt atcacctaat tcatctgtac
     1561 agactgtttg tattacacct gtagaatctt tcaaatcaat aaaaataatt ttcccgtgat
     1621 ctctcctagt ctcaactcaa ccatacaaat ctattgtctt accaacaaaa tttggcgtat
     1681 cttttatata tatagatttc attataaaaa tattttaatt tcattataaa catattttaa
     1741 tttcattata aaaatatttt aatttcatta taaaaatatt ttaatttata taatctaaaa
     1801 tcttatctaa atatcaacct atataatata gtttatcata ttttctattg aaattcaata
     1861 atcccatcca aatctgttct tttataagaa attccaaaat ctcgtaataa atttataata
     1921 aagtcagttg ggtgtccata actatttttg cctacttcaa taatagcttg tttgactaat
     1981 atattgttta aaatattgtc agataaagta ttttttgctc catgatgagg tacttttaaa
     2041 acatcaattg tttgaccctt caaaaattta tctacaacac catctaacaa ttttgctgta
     2101 atatcacctg taaataaaaa ttttttatta ttcaaattca acaaactaat aatagaaaaa
     2161 tcattatctg ccttaatgtt ttgtaaattt tgtatatctg gataaagaat ttttagaaat
     2221 gtatctgatt gacccaaata tataatagta ttagtagttc ctacaactat tggtattttt
     2281 tgcttacata atcttaataa taattctata taagtaccat tttgagaact aaaattattt
     2341 aatacaacta atcgtacttt ataataatct aaaattttta ataaaccttc aaaatgatct
     2401 acatctggat gcgtaatcaa agcaatatca atagttttgt caaataaaga agtcttttga
     2461 gacaaaggaa ctaaaacatt ttgtttaggg cctgcatcaa ttaaaatatt cccagctttt
     2521 gtttttatca attcgctatc tccttctcca actggtaaaa acaaaacctt tggtttaaaa
     2581 taaatataat taaaaatggc ataatatatt attatgttta tgactactaa acctccgatg
     2641 gcaacttttt tgtatttttc ataattttta tccatattgc ttttgtttat ttaagtccgt
     2701 tttattatta gatctaataa attaaaaaga aaaagttaga tacaaatgct tcttgtaata
     2761 ttttatcaaa aaaacaatta aaattaaata gtatatacac attactatta atgataatcc
     2821 accaatcgga taattggcat atttaacaga agcaaacaaa cttaccagaa ataaaataaa
     2881 ttttaaatat ccactaatta taatattcac aaaccaaaat aatgaacccc aaacaaaatt
     2941 caaacatacg ccaactacca ataaacataa caaaggcgta atcaagggca ctataattaa
     3001 attagccagt ggtgatatta aagaaatact attggttttg tatattaaaa atggcgtcat
     3061 accaataaaa atagataaca ctactgataa ggcaaatata acactttttc atttttcatt
     3121 tggaaaatat aatgtcaaaa catcagtaat tattggagaa atatagacta aacataaaac
     3181 agataaaaaa ctcaattgaa accccaaatc gacaaataat gtttgaggat taaacaataa
     3241 aatcacaaaa gaacttaata acaaagcatt ggtcatatta tatacacgat gattggcttt
     3301 acctataata tgtaacaaaa tcataatact cgccctcaat aaagatccag taaaaccaat
     3361 caataaaata aaaaataaaa taaaaatcaa attaatcgaa aatacaaaaa ttaaacccac
     3421 aggtaacata taaactaaat aattcaaaat atccgtaaac atagttaaat gcaaacccga
     3481 tacaactaaa atatggctca accctgcttg gcgaaacata ttatcaatat ccttattgta
     3541 tcccccactt ttacctacta aaatcataga agccaaaaat gattcacgtg gtaaaaacat
     3601 ttcttttatt ttagctataa ttgaattacg tcacataaac aaaaaatctt tgtgtaaaga
     3661 tggttctact atttgaattg ttccattgta tatagtataa actaatttat tgttcaaaaa
     3721 aacatcaaaa ggattataag tatttgcttg taactttccc ataagtaata ttttatctcc
     3781 tttatggata tcttgataaa atgggactga cactaaaata agatctttta aattcttagc
     3841 attatcttga tcttgataaa ttggttttac tgctaaatat tttttatctt ctatttctac
     3901 tggattacgc at
//
LOCUS       DAOTJA010000056         3568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_21823_length_3568_cov_5.045545, whole genome shotgun sequence.
ACCESSION   DAOTJA010000056 DAOTJA010000000
VERSION     DAOTJA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21823_length_3568_cov_5.045545"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..631)
                     /locus_tag="P5241_03670"
     CDS             complement(413..631)
                     /locus_tag="P5241_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32600.1"
                     /translation="MEAKIVSTLLFNPLLFGLSKKYKSAGLKQLLNYETNNFFTPAQL
                     ISFSIVNISISINDCLFFIYNLSSLNVN"
     gene            745..1179
                     /locus_tag="P5241_03675"
                     /pseudo
     CDS             745..1179
                     /locus_tag="P5241_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1360..1944
                     /locus_tag="P5241_03680"
     CDS             1360..1944
                     /locus_tag="P5241_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32601.1"
                     /translation="MGGSQGAQTLNDLILDGLSQFLEYFEVIHQTGQNNIEQVKREVD
                     VIFREYNINQNLKPFYHPVGFLQESQVPSINSLKDCLIASDIVVARSGSGSIFEIAAF
                     GKPSVLIPLPWASADHQTVNAFAYASSQSSPRAIVLEQENLKPNLLCNTLKNLVSDKQ
                     KLKEMSQNALKFAKKDASLSIARVVLQYIIDAYQ"
     gene            2069..2893
                     /gene="gltX"
                     /locus_tag="P5241_03685"
                     /pseudo
     CDS             2069..2893
                     /gene="gltX"
                     /locus_tag="P5241_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
     gene            2924..3259
                     /locus_tag="P5241_03690"
     CDS             2924..3259
                     /locus_tag="P5241_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32602.1"
                     /translation="MEDLIQKFTLTRIQKSGAIFNLQKLDFLNKHYISLKPKQELIEE
                     VLNYGTEFNFITLKEGSVILNSNNKSASMLQFTKYIETEQTRVNKYSDFFQTNFLFTD
                     LDYLAEDLC"
     gene            3281..3550
                     /locus_tag="P5241_03695"
                     /pseudo
     CDS             3281..3550
                     /locus_tag="P5241_03695"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1273 a          434 c          604 g         1257 t
ORIGIN      
        1 gaatagagga tatggaacag ttgtagaatt gtctcctctt aaaatagacg ccgaaatata
       61 cccagaagaa ggtagcaatc gtttatatgt gcgttctacc atttctatag gtgataatca
      121 atgatacttt ataacagtaa cttttactcg agcatcacta ttacgtattt atatcaatgc
      181 tgtttttaat acatccacag atatttctca atataaaaat tattcagtaa atacaactta
      241 tgacctatat ataggaggat ggggaggcac caatacatct gctaattttt tgggctatct
      301 tgatgacgtc cgcatttata atagagcatt aagtgcggga gaaatacagt atttatatac
      361 taaaacacaa gcacgatata agtaatagtt taatttacta atttaccaat ttttaattta
      421 catttaatga tgataaatta taaataaaga ataaacaatc atttatggaa atagagatat
      481 taacaatact aaaagatatt agttgagcag gggtgaagaa attattagtt tcataattta
      541 atagttgttt tagtccagcg cttttatatt tttttgataa accaaacaat aatggattaa
      601 ataaaagggt tgatacaatt tttgcttcca ttttcagtcc tgagctctaa tctctattct
      661 tttgaaatta aaaaattttc tgacttatat tctttttaaa attacaaaat taaaaaattt
      721 atattaaatt ataattatta cattatgtta ttttcgccga ataaaaatat taaaattgta
      781 ttagctggtg gtggtagtgg gggtcatgtg tttccaataa tttctgtttg tgaagcatta
      841 aataaatata taccagaatt tgaacgtcaa ggtttatctg ttgagttgat atattttggt
      901 gccaatgact ttactttaga ttatgtaaaa cataaaaata tttctataaa agttatttct
      961 tgcggaaagg ggatagggga tttttggaaa actattatag gattttttca agctttttga
     1021 aatttgttta ttgtaatgcc tgatgcaata tttgggaaag gtggttttgc tactgtacct
     1081 acactgagta taggggtttt gtttaggatt cctatattta ttcatgaatc tgatagtata
     1141 cccggtaaag ttaaccaatt ttttggtcgt tttgctaagg gaatatttat ttcttttgaa
     1201 aaaagtgctg attattttgt tcaaaaagat aaattgattt tatcaggcaa tcctattcgg
     1261 gattttttga atattccaga tagtgagtta gaaaaaatag ataaaaatca aactaaaaaa
     1321 ttattaggac tacaaacaaa aaagccagtt ttgttagttt tgggtggttc acagggtgct
     1381 caaactttga atgatttgat actcgatggt ttatcacaat ttttagaata ttttgaagtt
     1441 attcatcaaa ctggtcagaa taatattgaa caagtcaaaa gagaagttga tgttatattt
     1501 cgtgaatata atataaatca aaatttaaaa cctttttacc atccagtcgg atttttacaa
     1561 gaatcacaag ttccatctat taattcttta aaagattgtt taattgcctc tgatattgta
     1621 gtagccagaa gtggcagtgg cagtattttt gaaatagctg cctttggtaa gccatcagtt
     1681 ttgatacctt taccttgggc aagcgctgat caccaaacag ttaatgcttt tgcttatgct
     1741 tcatcgcaat catcgccaag agcaatagtt ttggaacaag aaaatttaaa gccaaatctt
     1801 ttatgcaata cattaaaaaa tttagtatca gataaacaaa aattaaaaga aatgtcgcaa
     1861 aatgctttaa aatttgctaa aaaagatgcg agtttgagta ttgctcgagt agttttacaa
     1921 tatattattg atgcttatca ataatatatt aggtaagaat tttgaatatt tggattggtg
     1981 gtaaaggtta aaataaaaaa taaattatta agttattttt atgtctttgg atcctaaaac
     2041 aataaaattt gaaaatttgc gagtgcgtat ggcgccaagt cctacaggtt tgttacatat
     2101 aggtacagcc agaactgcgt tgtttaatta tttaactgcc aaacattacg gtggtaaatt
     2161 tgttttaaga attgaagata cagatttgga aagatcaaca aaagaatttg aaaaaaatat
     2221 tattgatggt ttaaaatggt tgggaataaa ttgagatgaa gggccagata ttgggggtcc
     2281 ttatgctcca tataatcaaa atgatagaag agatatttat aaaaaatatt tacaacaatt
     2341 attagatgaa ggcaaggctt attattgttt ttgtacccaa gaagaattgg aaatacaaaa
     2401 acaagaaatg attactcacg gagaggctcc aagatatatt ggtcgttgtg ctgaattacc
     2461 ccccgaaaca gttcaaaaat atttagcagc aggcaaaccc tcagtaattc gttttcgtat
     2521 gccaaagatt aaaattagct ttaaggatat tatacgcaaa gaagtcaaaa ctgatttgaa
     2581 tttgattggt gatattgtta tagccaaaaa tttgaatgaa cctttgtata atttttctgt
     2641 tgttattgat gatcatttga tggatatatc tttggttatt cgtggagaag atcatattac
     2701 caatactcct aaacaaattg ctttgtataa agcttttgat tgggatacac ctatgtttgc
     2761 tcatttgcct ttgattttga acagtaacaa aacaaaatta agtaaaagag ataatattgt
     2821 ttctattgat gattatcgtc aagcaggata tttaccacag gctattgtta attttatagc
     2881 tttgttgggt tgacatgatt catcgggtaa tgatattttt tctttggaag atttaattca
     2941 aaagtttact ttgacaagaa ttcagaagtc tggggcgata tttaatttac aaaagttaga
     3001 ttttctgaat aagcactata tttctttaaa accaaaacaa gaattgattg aagaagtttt
     3061 aaattatgga acggagttta attttataac attaaaagag gggagtgtaa tattaaattc
     3121 aaataacaaa tctgctagta tgttacaatt tacaaaatac atagaaactg aacaaacaag
     3181 agtaaataaa tattcagatt tttttcaaac taatttttta tttactgatt tagattattt
     3241 agccgaagat ttgtgttgaa aagatcagac caaagaagaa gctattgaag ctcttgacaa
     3301 atcatatcat ttattatata gtttggttga ggaacaattt gatctggata ttataaaaat
     3361 tcagtttgaa gagcttatta aatataatga aaaatataaa cttaataaag gccttatttt
     3421 atggccgttg agaatggctt tatctgggct aaaatattcg ccaagtccat acgaactttt
     3481 agctgtttta ggacgcgaag aatcattaag acgtataaag atagcccaag accgtctcgc
     3541 ggcagtttaa aagccattta aacgcgtt
//
LOCUS       DAOTJA010000057         3565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_21843_length_3565_cov_3.505413, whole genome shotgun sequence.
ACCESSION   DAOTJA010000057 DAOTJA010000000
VERSION     DAOTJA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21843_length_3565_cov_3.505413"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="P5241_03700"
     CDS             <1..421
                     /locus_tag="P5241_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HRV32603.1"
                     /translation="GFGANLTAEEEIALTKDLKSISDRYNIKMYACAEDNLLKPTSVE
                     KAHCVDYDLIQKVSGNDFKYKEVPSRIECGCIESKDIGYYDSCPHGCIYCYANMDPEK
                     ALENARMYLKNGFPYDNLPRKNNNVLSTGKNQIKMDI"
     gene            complement(587..2509)
                     /locus_tag="P5241_03705"
     CDS             complement(587..2509)
                     /locus_tag="P5241_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRV32604.1"
                     /translation="MIKTKVTIILFILFTTLLNAQKSGYIVDDFHQHTTYSDGWYSME
                     TMMYMNNKFGLDWWATNDHGGGFPRDGRGPLRTNLPHTLDTVWNSIAIDAKWWDSYQP
                     NPIIGTNKTSYNHQVMWRWQSLRDFQMHDVLNARNKYKDKVIILGYEWNMPGHEHCTM
                     GIISNQFDNNPNANPMAEFEYKFDNSDTDTTGGKLQGWAKSILTGHNKALEAAKWLQQ
                     KYPKTSYIIPAHPERKSLYKIEDFRDLNSAAPDVCFGFESMPGHQKGPARGEYSPSSK
                     SDGGCTYGGTGIYAAKVGGLWDALLGEGRRWWLFASSDCHSVGTDELNGPDFWPGQYQ
                     KTFTYVSDKTNPQAIIDGLRSGNSWVVTGDLIDSLDFTVNGAKMGEYAKPINGNVTII
                     IKVRDPQKNNNNIFSEYKNPVLNHIDLIAGKVTGRLYNNQPGYNDPTNPTTKVIARFD
                     AVGGIKDGNNIISQKWEDKGNGWKEMSITLTNVTDKMYFRLRGTNHGLNVPNETDING
                     NPLPDTLIPATAATAFADLWFYSNPIFCGENITDVKNEYKPNNEINTYKLEQNYPNPF
                     NPSTTIEYYVPTNSHVNISIYDINGKKVKTLVNEIKNKGNYKINFDASDLSSGVYYYT
                     IKGNNFSKTMKMNLLK"
     gene            2831..>3565
                     /locus_tag="P5241_03710"
     CDS             2831..>3565
                     /locus_tag="P5241_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV32605.1"
                     /translation="MFKSIAAREPLSKRIVAEIEEAIRQKRIPVNSQLPSEKELCAQF
                     NVSRTVLREALKILGGMGLITIRKGKGVFVNDISTSTVSSHIERYLLMKLEKQQILDV
                     HYVREIIEPNIVATAALTRTDEDIQKLKDNYEKFKNPNIDYKERSALDGEFHLLIAKA
                     TKNSLLPMILEPIYNVMYEIRSAVYPAVKGSGHSAILWHKKIIDAIISKDVESAKTSI
                     IRHLEISREHTKKLLKKTIKESEIRKN"
BASE COUNT         1217 a          510 c          535 g         1303 t
ORIGIN      
        1 cggcttcgga gctaacctta ccgcggaaga agaaattgca ttaacaaaag atttaaaatc
       61 aatatcagat agatataata ttaaaatgta tgcttgtgca gaagataatt tgcttaaacc
      121 aactagtgtt gagaaggctc attgtgttga ttatgattta attcaaaaag ttagtggtaa
      181 tgattttaaa tataaagaag ttccatctcg tattgagtgt ggttgtatcg aaagcaaaga
      241 tattggctat tatgatagct gtccacatgg gtgtatctat tgttatgcga atatggatcc
      301 agaaaaagct ttagaaaatg cacgaatgta tttaaaaaat ggatttccat atgataattt
      361 gccaagaaaa aataataatg ttttaagtac aggtaaaaat caaattaaaa tggatatata
      421 atatatgtat aattataggc taatatttat tttaaaaaat attataaaca attaattatt
      481 tatatttcaa taaaatattg acgaaagtaa agagaaatag aataatatat ataaataaaa
      541 agggggtaat taaccccctc taattattca ttgagaatgg ctatgatcat tttagtaaat
      601 tcattttcat tgttttactg aaattgtttc ctttaatagt gtagtaataa acaccactgc
      661 ttaaatcaga agcatcaaag tttatcttat aattaccttt attttttatc tcatttacaa
      721 gagtttttac ttttttacca ttgatgtcat aaatagaaat gttaacatga gagtttgttg
      781 gtacataata ttcaatggta gtagaaggat taaatggatt aggataattt tgttcaagtt
      841 tatatgtgtt tatttcatta ttaggtttat attcattctt cacatctgtt atattttcac
      901 cacaaaaaat aggattagag tagaaccata aatctgcgaa agctgttgct gcggtagctg
      961 gtattaaagt atcaggtaat gggttgccat taatatccgt ctcgttagga acatttaatc
     1021 catggttagt acctcttaat ctgaaataca ttttatctgt aacatttgtg agtgttatac
     1081 tcatttcttt ccatccattg cctttatctt cccatttttg gcttattata ttattcccgt
     1141 cttttatacc gccaactgca tcaaatctcg caataacttt tgtagttggg ttagttgggt
     1201 cattatatcc tggttgattg ttgtataacc ttcctgttac tttaccagct attaaatcaa
     1261 tatgatttaa tactggattt ttatattcag agaaaatatt gttattgttt ttttgagggt
     1321 ctcttacttt tattataatt gtaacatttc cgtttatagg tttggcatat tcacccattt
     1381 ttgcaccatt tacagtaaaa tcgagtgaat caattaaatc accagtaact acccaactat
     1441 tacccgatct taaaccatct attattgcct gtggatttgt tttgtcagat acataagtaa
     1501 atgttttctg atattgtcct ggccaaaaat ctggaccatt aagttcatct gttcctacac
     1561 tatgacaatc agagctggca aagagccacc atcttcttcc ttcacctaaa agagcatccc
     1621 atagccctcc aacttttgca gcatagattc cagttcctcc gtatgtgcat cctccatcag
     1681 attttgatga aggtgaatat tctcctcttg caggaccttt ttgatgtcct ggcatacttt
     1741 caaaaccaaa acaaacatct ggtgcagcac tgtttaagtc tctaaaatct tcaattttat
     1801 ataaactttt tctctctgga tgtgctggta ttatataact tgttttgggg tacttttgtt
     1861 gtaaccattt tgcagcttct aatgctttgt tgtgacctgt taatatactt tttgcccatc
     1921 cttgtaattt acctccagtt gtatcggtat cactattatc aaatttatat tcaaattcag
     1981 ccattgggtt tgcatttgga ttattatcga attggtttga tattattccc atagtacaat
     2041 gttcatgacc cggcatattc cattcataac ctaatattat tactttatct ttatacttat
     2101 ttcttgcatt taatacatca tgcatttgga agtctcttaa agattgccat ctccacataa
     2161 cctgatgatt gtaacttgtt ttatttgtac cgattattgg attaggttga tatgaatccc
     2221 accatttagc atcaattgca atagaattcc atactgtatc aagagtatgt ggtaaattag
     2281 ttcttaaagg tcctctacca tctctaggaa aacctccacc gtgatcattg gttgcccacc
     2341 aatctaaacc aaatttgttg ttcatataca tcatagtttc catagagtac cagccatcag
     2401 agtatgtagt gtgttgatga aaatcatcta ctatataacc tgatttttgt gcgtttaata
     2461 atgtggtgaa aagtataaat aaaataatag taacttttgt tttgatcata aattactcct
     2521 atgtttttta aagttcggtc aaatatattt tgtaactatt attaaaaaat taagattgga
     2581 ttaataaatt ttaaaaaaat tatttatttt aagttaaggt attgttaaaa taatattatt
     2641 atcttctaat attgtagaac taaaaaatga aaatattttt aatatattat aatcataatt
     2701 atatttgcga taattttcct ttttgagtta ttttaaccaa tttttttaaa gcgattttac
     2761 ttgacaaaaa aaaatattat agttatattt aacatgtatg atatgtgata tgtgatatag
     2821 gagataataa atgtttaaat caattgctgc aagagagcct ctttctaagc gaatagttgc
     2881 agaaatcgaa gaagccataa gacagaagag aatccctgtt aattctcaat taccttctga
     2941 aaaagaatta tgtgctcaat ttaatgtaag cagaactgtt ttaagagagg cgttaaaaat
     3001 tttaggtggt atgggtttga ttactataag aaaaggtaag ggtgtttttg taaatgatat
     3061 ttctacaagt actgtatcaa gccatatcga aagatattta ttaatgaagt tagaaaagca
     3121 acaaatactt gatgttcatt atgttcgaga aataattgag ccaaatattg tagctacagc
     3181 agcattgaca agaactgatg aagatatcca aaaattgaaa gataattatg aaaagtttaa
     3241 aaatcctaat attgattata aagaaagatc cgctttggat ggtgaatttc atttacttat
     3301 agcaaaagca acaaagaatt ctttattacc aatgatttta gaacctattt ataatgtaat
     3361 gtacgaaatt agatctgcag tatatcctgc tgttaaaggt tccggtcatt ccgcaatttt
     3421 atggcacaaa aaaattattg atgcaataat aagtaaagat gttgaatctg caaaaacaag
     3481 tattataaga catcttgaaa tatcacgtga acatacaaaa aaactcttaa aaaaaacaat
     3541 aaaagaaagt gaaataagaa aaaat
//
LOCUS       DAOTJA010000058         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_22171_length_3525_cov_4.148703, whole genome shotgun sequence.
ACCESSION   DAOTJA010000058 DAOTJA010000000
VERSION     DAOTJA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22171_length_3525_cov_4.148703"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            757..1245
                     /locus_tag="P5241_03715"
     CDS             757..1245
                     /locus_tag="P5241_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRV32606.1"
                     /translation="MISKNVVEALNNQINHELASGYLYLSMASYADSINFGGFAHWLK
                     LQAKEEVEHAMKIYDYLNDCGEKVELKGIEQPETKFNSLKEVFEKVLAHEKKVTSLIN
                     KLYELAVKENDYATQIKMQWFVSEQIEEEKNASDILEQLKNIGDHYGSLIMLDRHLAS
                     RQ"
     gene            1307..1666
                     /locus_tag="P5241_03720"
     CDS             1307..1666
                     /locus_tag="P5241_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV32607.1"
                     /translation="MSKDYFCHIQLPAKDLNRVKNFYKTVFNWNFESLQKDIILFNPP
                     DGIGGSFKLAKETSNNTCILPFICVEDIEKTLELIIENNGTLIEIKTEVPGLGYIAKF
                     SDTEGNVLALLEEYKNE"
     gene            1669..3132
                     /locus_tag="P5241_03725"
     CDS             1669..3132
                     /locus_tag="P5241_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRV32608.1"
                     /translation="MTYNISVDINNKQNLINPLLYGSFAEHLGKCIYGGIWVGENSSI
                     KNERGFRIDVLDCLKEIELPVIRWPGGCFADTYHWIDGVGPKNKRPRRYNLWWKQPES
                     NAFGTDEFMQLCHLLNSEPYLAINVGSGSVQEALNWIEYCNSSKNTEFAELRVKNGHP
                     KPYNVKYWGIGNENWGCGGAMSPEYYADLYRRFATYIRQQTDSNVKLIACGSNTDFDD
                     WDERVLKNIQTKYFDLVDFISLHVYTGWNIEQNNLSEQDYYKIIKDISITKNKIEKAI
                     QLCNIYSKNKNIKVILDEWGLWYKEATVNNGLFQEGLLLDAIFAALNFHLFHNYSDDL
                     FMTNLAQAVNVLQSFILTIGNNIVRTPTYYLYKMYKPHRGKRVVKLNSNLPKIKLTDD
                     DYVNALSISGSISEDNKEIFITIVNIDLEKDYYINLNSFPNYSLIDAFVLTANDFRER
                     NNFFSPNTIMLVKREKNDLKDILIKKHSLNSFLFKKI"
BASE COUNT         1296 a          457 c          597 g         1175 t
ORIGIN      
        1 ccgatatgtt tgtagaaaaa cgcctaaaaa tatatatcaa gcaccgtagg tgcgatagat
       61 aaaaacaggt atcagggcta cgccccttgt acatttatat aacatttatt ctacaaacat
      121 ttcggtgcta cgcacctttt atttttacta atcgatatcg ttttggttac acaaggggca
      181 gagccccgat atggttgtag aaaaaccgag aacaaaaaat atcaagcacc ataggtgcga
      241 cataaaaata tgtgtcaggg ctacgcccct tgtatgttta tataacattt attctacaaa
      301 catttctgtg ctacgcacct tttattttta ctaatcgagg tcattttgtt taccgtaagg
      361 ggcagcgccc cgatatgttt gtagaaaacg caaataaaaa aaataaagca ccgtaggtgc
      421 gacacaaaat aattatacat ttctgtgcta cgcaccttta tttttactaa tcgaggtcat
      481 tttgtttacc ataaggggca gcgccccgat atgtttgtag aaaaccgtga acaaaaaaaa
      541 taaagcaccg taggtgcgac acaaatttta atattataaa atattttgcg aatcgtgaat
      601 gcttcgatta catctatgaa tattttgaaa tgaaaaataa tttgtttaat attgaacttt
      661 ttataaaaaa tctatgttat gtgttgtaga aatatttgaa ttgaaaaaga tatttctaat
      721 tattatctta tttacacaaa cataggagac tgaaaaatga ttagcaaaaa tgttgttgag
      781 gcgctaaaca atcaaattaa tcacgaattg gcatcaggct atctttattt atcgatggct
      841 tcttatgccg actcaataaa tttcggtggc tttgctcatt ggttaaaatt acaagcaaaa
      901 gaagaagttg agcacgctat gaagatttat gattacttga atgattgcgg tgaaaaagta
      961 gaattaaaag gaattgaaca accagaaact aaatttaatt cattgaaaga agtatttgaa
     1021 aaagttttag cacatgagaa gaaagtaaca tctttaataa ataaattata tgagttagca
     1081 gttaaagaaa atgactatgc aactcagata aaaatgcaat ggtttgtttc agagcaaata
     1141 gaagaagaaa agaatgcgtc tgatatttta gagcaattaa aaaatattgg cgatcattat
     1201 ggttctttaa tcatgttaga tcgacacctt gcttcaaggc aataaatatt atggcttttc
     1261 atttttataa tggaaagcct ttttattatt attttaagag agcaacatgt ctaaagatta
     1321 tttttgtcat attcaattgc cagcaaagga tttgaataga gttaagaatt tttataaaac
     1381 tgtatttaat tggaatttcg agagcttgca aaaagatatt attctattca atcctcctga
     1441 tgggataggt ggctctttca aattagcgaa ggaaacttct aataatactt gtatcttacc
     1501 ttttatttgt gttgaagata tcgaaaagac tttagagtta ataattgaaa ataatggaac
     1561 gcttattgaa ataaaaactg aggtgcccgg tttgggttat atagccaaat tttctgatac
     1621 ggaaggtaat gtgcttgctt tattagaaga atataaaaac gagtaaaaat gacttataac
     1681 atttcagtcg atattaacaa taaacaaaat ctcataaatc ctttattgta tggttctttt
     1741 gcagaacatt taggtaaatg tatttatggt ggtatatggg ttggagaaaa ttcctctata
     1801 aaaaatgaaa ggggatttag aatagatgtt ttagattgcc taaaagaaat tgaattgcca
     1861 gtaataagat ggcccggtgg ttgttttgct gatacatacc attggattga tggagttgga
     1921 cctaaaaata aaagaccaag aagatataac ctttggtgga aacaaccaga aagtaatgct
     1981 tttggaacag atgaatttat gcagttgtgc catctactta attccgaacc atatttggct
     2041 attaatgttg gttcaggttc tgttcaggaa gcgctaaatt ggatagaata ctgtaactca
     2101 tcaaaaaata ctgaatttgc agagcttaga gtaaaaaatg gtcacccaaa accttataat
     2161 gtaaaatatt ggggtattgg taacgaaaac tggggttgcg gtggtgcaat gtctccagaa
     2221 tattatgcag atttgtatag acgctttgca acttacattc gtcagcaaac agatagcaat
     2281 gttaagttaa tagcctgtgg ttcaaataca gattttgatg attgggatga gagagttctc
     2341 aagaatattc aaacaaaata ttttgacctg gttgatttta tttccttaca tgtttatact
     2401 ggttggaata tcgagcaaaa taacctttct gaacaagact attataagat tattaaagat
     2461 ataagcatta caaaaaataa aattgaaaaa gcaattcagc tctgcaatat atatagcaaa
     2521 aataaaaata ttaaagtgat tcttgatgaa tgggggctgt ggtacaaaga agctacggta
     2581 aataatggat tgtttcagga aggattatta cttgatgcta tatttgcggc gttaaatttt
     2641 catcttttcc acaattattc tgatgatttg tttatgacaa acttagctca agctgtaaat
     2701 gtgcttcaat catttatctt aacaataggt aataatatag tacgaacacc tacatactat
     2761 ctttataaaa tgtataaacc acatagaggt aaaagggtag tcaaattaaa ttccaacctg
     2821 ccaaaaataa agttaactga tgatgattat gtaaatgctt tatcaatatc cggttcaata
     2881 tctgaagata ataaagagat ttttatcaca attgttaata ttgatttaga aaaagattat
     2941 tatattaatt taaattcttt tccaaattat agtttgatcg atgcttttgt gttaactgct
     3001 aatgatttca gagaaagaaa taacttcttt agtccaaata ccataatgtt agtaaagaga
     3061 gaaaaaaatg atttaaagga tattttgata aaaaagcata gcctaaattc ctttcttttt
     3121 aaaaaaatct aatataaaat tattttataa atcacattct gggattatca taaaatttat
     3181 ccaatacagt catattattt atacctttat taatagtaaa cacaattata aagtttataa
     3241 aatctaattc tacgtttccg ggcagtagag gtaaggcttt ttaatctgat ttttcaataa
     3301 aagagatgtg gttctaactg aaatatgtct ttgaaagtga ttaaagtttt gctacttatg
     3361 cagaagtaaa ttatatatag atagttttga gatgaaactt atataaatat ttgtaaattt
     3421 acttaataat tgtaaataaa caatataata atttgcctat ctccaaataa cacttaagca
     3481 aaaaatcgaa gtgttatgta gtgttattag tgttataagt gttat
//
LOCUS       DAOTJA010000059         3392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_23203_length_3392_cov_5.547797, whole genome shotgun sequence.
ACCESSION   DAOTJA010000059 DAOTJA010000000
VERSION     DAOTJA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23203_length_3392_cov_5.547797"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="P5241_03730"
     CDS             complement(<1..757)
                     /locus_tag="P5241_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRV32609.1"
                     /translation="MNISQNKINANELELKISLQKEDIQHNLPQVASDIGKDLEVPGF
                     RKGKVPFEVIEQKFGQEYLYEEAAKISLKDAYANYIIEHKIDVVDQPEVNFSKFVLDG
                     DVEFIAKVKILPEVDLPNYKKIGQEVSKEKKAMTVEDKELQDTLKYIVESRATYTSID
                     EPGKEGYIAEIDYIISKTSLDTDNIIEKQEKYRVVIGQEPIFQELNKELIGLKKGDEK
                     EITITFPSDYIEKDLQGTTAKMKIVVKDILEKKL"
     gene            complement(834..1451)
                     /locus_tag="P5241_03735"
                     /pseudo
     CDS             complement(834..1451)
                     /locus_tag="P5241_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1470..2435)
                     /locus_tag="P5241_03740"
     CDS             complement(1470..2435)
                     /locus_tag="P5241_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HRV32610.1"
                     /translation="MLDLKDIKGAIRQIVLDKGLPEEKIWQAIELAMAKAYKKEYLNR
                     YAQVRATIDKDTGEVKFFRVFLVVDESMIIPAKSLEEQVNQEDQNNLTSTSAKAQTKI
                     NKNIKPIDTPVNISSNQEDSTNSPETGWEAENEQDLENADIDEATKKIRFNPERHIMI
                     DDAKKINKNIKVGEELIIALKFEESFSRIAAQSAKQTIIQQIRELEKETTLNEFENKE
                     GEIISGVIQKIDRGTVIVHLGKAIGILPAKEAIPFERFELEERKKFLILGIREAKDSL
                     VVLLSRVHPQFIIKLLTLEVPEIQEGIVEIKAISREPGYRSKIAV"
     gene            complement(3116..3322)
                     /locus_tag="P5241_03745"
     CDS             complement(3116..3322)
                     /locus_tag="P5241_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32611.1"
                     /translation="MEKIMNIIVLAAVVYLGSNVFFSFAGFPSESMYFIDGMVPSVGT
                     MSAVCTGIFIFAAGFYLCCKYKKK"
BASE COUNT         1034 a          580 c          393 g         1385 t
ORIGIN      
        1 gtaatttttt ttctaaaata tccttaacta ctattttcat cttagcagtt gtgccttgta
       61 gatccttttc aatataatca ctagggaaag taatagtaat ttctttctca tcaccctttt
      121 ttaaaccaat taattccttg tttaattctt gaaatattgg ctcttgacca ataacaactc
      181 tgtatttctc ttgtttttct ataatattat ctgtatccaa agatgtctta gagattatat
      241 aatcaatttc tgctatatac ccttctttgc ctggttcgtc aatacttgtg taagtagctc
      301 gggattctac tatatatttc agagtgtctt gtaattcttt gtcttctact gtcattgctt
      361 ttttctcttt tgatacttcc tggccaattt ttttataatt tggtaaatct acttctggca
      421 aaattttaac tttggcaata aactctacgt caccatctaa aacaaattta gaaaaattaa
      481 cctctggctg atcaacaaca tcaatcttat gctcaataat ataattagca taagcatctt
      541 ttagagatat ttttgcagct tcttcataaa gatattcttg cccaaatttt tgttcaataa
      601 cttcaaaagg taccttgccc ttacgaaaac caggtacttc taaatcctta ccaatatcag
      661 aagcaacttg tggtaaatta tgttgtatat cttctttttg gagtgatatt tttaattcca
      721 attcgtttgc atttattttg ttttgagaaa tattcattat gttattttaa tattttatta
      781 aaaaattatt cgaattaaat cactaacaaa caatatataa ttaaaattaa taattaatct
      841 tctttcttta ttttaatttc tgaatcattg gtatacatag tatcgttatt attctttgat
      901 atacgctttg actttttctt ggtaacagta atttgttcat cttgttcctc atcatcattt
      961 atttccttgt tactatattc atcattatac tcatcatcgg gatcatcatt attatcgtta
     1021 ttatctatat tttgtgttag atcaacatta tttttttgat caagatcatc atcatagcta
     1081 ctattatata tatcaccatc aatattatta tctgacaaag ttttgctatc agtattggta
     1141 ttttgttcaa aatctggggc aacagtagag taaactctta tattccatcc ggtcaatttt
     1201 gaagctaatc tgacattttg accattttta ccaatcacgg ctgataataa ttctggattt
     1261 acaaacactt taacttcgcg acgaacagga tcaatttctg tttcttttat ttttactggc
     1321 gataaagcat tggctacaaa tatagcaggt tcttcagaat attctacaat atcaattttt
     1381 tcattgggta tttcttctaa tacattattt attcttactc cctttggacc aatacaagaa
     1441 cccacagcat ctacttctgg ttgattgctt cacacagcta ttttacttct gtatcccggc
     1501 tctcgactta tggcttttat ttctacaata ccttcttgaa tttctggaac ttccaaagtc
     1561 aaaagtttaa taataaattg tgggtgaacc cgagagagta aaaccacaag tgagtctttg
     1621 gcttcacgaa tacctaaaat taaaaatttt ttgcgttctt cgagttcaaa acgttcaaat
     1681 ggtatagctt ctttggcagg caaaatacct atagctttac ccaaatgaac tattactgta
     1741 cctctatcaa ttttctgaat aacaccagaa ataatttctc cctccttgtt ttcaaattca
     1801 tttaatgttg tttctttttc taattctcta atttgttgaa taattgtttg tttagcgctc
     1861 tgggcagcaa tacgactaaa tgattcttca aatttcaaag cgattattaa ttcttcacca
     1921 actttaatat ttttgttaat ttttttagca tcatcaatca taatatgacg ctctggatta
     1981 aaacgaattt tttttgtggc ttcatcaata tccgcgtttt ctaagtcttg ttcattttct
     2041 gcttcccaac cagtttcagg gctatttgtg ctatcctctt gattactaga tatattaacc
     2101 ggagtatcta ttggtttaat atttttgttt atcttagttt gtgctttcgc acttgtagat
     2161 gttaaattat tttggtcttc ttgatttact tgttcttcta atgacttagc aggtataatc
     2221 atagattcat ctacaactaa aaatactcga aaaaatttta cttcaccagt atctttatca
     2281 atagtagctc gaacttgagc atatctattt aaatattctt ttttgtaagc tttggccata
     2341 gccaactcaa ttgcttgcca aattttttct tctggcaatc ctttgtctaa tacaatctgc
     2401 cttattgctc cttttatatc ttttaaatct aacatatttt aacgtttatt ttttattctc
     2461 tttttttaaa cttatttcga ctatttttaa atctatataa aaatcgtcca tatccagtac
     2521 taaatttttt catattcgtt tacaaattgt aatatcgttt ttaaccttat atgcttcaaa
     2581 tagacaaatt taagaaaact ctatttttcg taataaaaca atacttattc ttatattgac
     2641 aaaacgacaa aacaatattt ataattagca gtatatccta tctacctata tatacatata
     2701 cctattatat aagttataat actgtatact ttctaaaata taaacgaata taatataggt
     2761 tttgaaaaga gtaggtagat ttttaaacaa ttggtcggct ttatgggccg accaactaat
     2821 atgcttaata catttttata ctaacataag ttttaaaaaa atgcaagtct tgtgcttata
     2881 tttgttttac atatttaatg ttttataaaa ggtcaaagtg tttggactga tccaacttgg
     2941 tttgtctttt tacataaaaa cctctaccaa atttaaatca acaaaaaata cttttatgaa
     3001 tattatattt ctgtttggtg tttctaaata tttgtacttt tgctttttaa atgtaaaaag
     3061 gagatattgc taactcttat gcaatatctc cttgtgttat ttgaacttgt tttttttatt
     3121 ttttcttgta tttgcaacag agataaaatc ctgctgcaaa tataaaaatt cccgtacaaa
     3181 cagcagacat ggtgcctacg ctcggcacca taccatcaat aaaatacata ctttcgctgg
     3241 gaaacccagc gaaagaaaaa aacacattgc ttcctaaata tacaacagca gccaatacaa
     3301 taatattcat aattttttcc attttttctc ctcttctcct aatttttatt tttgaagtta
     3361 cagaaggtaa tctgagaatt tttaattata ca
//
LOCUS       DAOTJA010000060         3290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_24141_length_3290_cov_4.386399, whole genome shotgun sequence.
ACCESSION   DAOTJA010000060 DAOTJA010000000
VERSION     DAOTJA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24141_length_3290_cov_4.386399"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..348)
                     /locus_tag="P5241_03750"
     CDS             complement(94..348)
                     /locus_tag="P5241_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRV32612.1"
                     /translation="MHKENLAMPHIKDSKDSKQIAEKPKEQKNPQSFEEYYNMYKDDI
                     ARVALYYTKNKDDAEDLVQNTLLKAYKHFSKFNKKTNFKS"
     gene            complement(396..1565)
                     /locus_tag="P5241_03755"
     CDS             complement(396..1565)
                     /locus_tag="P5241_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV32613.1"
                     /translation="MKIIFGLEVFLPHISGVTNIVSDLANYFSLDQSNEVFVITSSEN
                     NKFSRESRGKNYTIIRLPSFKVPFKENIRASYFSSFRAYDIIKKIKPDIIHIHDGAFI
                     SFALAIYAQRHNIPVVFTQHANLSFPSYFVKFGKKTVKKLYGAYLINFLNNYCELVVA
                     PSNSIKKELIENGCDTPVEVVSNGIDLNFYKPNLVLHKKHTIPQILYVGRIDQDKNLS
                     TFIKSIPLILDKTQAQFIFVGQGDLRKEFIEYFNKTSYNNYVTFVDSVKPNSIELLSF
                     YQSADVFVMPSCIETQSLATMEAMACGLPIVAANSGALPELVKNNKNGFLIPPYNISA
                     FAHAIIKLTKNPQKRADFAKNSLQFIKKHNRSNTFQTLQNIYSHLIQNNTKLDIK"
     gene            complement(1673..1743)
                     /locus_tag="P5241_03760"
     tRNA            complement(1673..1743)
                     /locus_tag="P5241_03760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1709..1711),aa:Ala,seq:cgc)
     gene            complement(2301..2645)
                     /locus_tag="P5241_03765"
     CDS             complement(2301..2645)
                     /locus_tag="P5241_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HRV32614.1"
                     /translation="MLGTQNTIKRKIKIGVLGSTFNPPHFGHLLLAKTALQKFHLSKV
                     LLMVCAIPPIRKKDLAPLKHRVKMAELLAKYDKHFEVSDLEIKRARHGKKSYTLDTYK
                     ELQQLYPNATIY"
     gene            complement(2914..>3290)
                     /locus_tag="P5241_03770"
     CDS             complement(2914..>3290)
                     /locus_tag="P5241_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32615.1"
                     /translation="YCYQDANLKNISIAPSSFYLPMNGNSSLAISDTLIPYMAKYQKI
                     SGGSGNLNFSFENPPDNLIKTIPYIIIYKNGLKQLFFLDFQKSNSQIIVVPDFNKEVI
                     GLVIIPQLSRQDKNYSTRSIFK"
BASE COUNT         1097 a          456 c          497 g         1240 t
ORIGIN      
        1 aacaatatca aaattttgat ttaaccctaa tatgttttgc tcatttttat gttttatctg
       61 ggttaaataa gtattttcta taattttaaa tattcaagac ttaaaattgg tttttttatt
      121 aaatttggaa aaatgcttgt aagcttttaa aagcgtattt tgtacaagat cttcggcatc
      181 atctttattc tttgtataat aaagagcaac ccgcgcaata tcatctttat acatattgta
      241 atattcttca aaagattgtg ggtttttttg ttctttgggt ttttcagcaa tctgttttga
      301 atcttttgaa tcttttatat ggggcatcgc taaattttcc ttatgcatat ttcatattta
      361 taatttttat tattatttgt atactatcat attagttatt ttatatcaag ttttgtatta
      421 ttttggatta aatgcgaata aatattttgc aaagtttgaa aagtattaga tctattatgt
      481 tttttgataa actgtaagct atttttagca aaatccgcgc gtttttgcgg atttttagta
      541 agcttaataa tggcatgcgc gaaagcagat atattgtacg gaggaattaa aaatccgttt
      601 ttattgtttt ttactaattc aggaagcgca ccgctattgg cagcaacaat cggtaaaccg
      661 caagccattg cttccatggt agctagactt tgagtttcta tacaagaagg cataacaaat
      721 acatcggcag attgataaaa agataaaagc tctatgctat taggttttac tgaatcaaca
      781 aatgttacat aattgttata agatgtctta ttaaaatatt ctataaattc ttttcttaaa
      841 tcaccttgcc caacaaatat aaactgagct tgtgttttat ctaatatcaa gggaatactt
      901 ttaatgaaag tacttaaatt tttatcctga tctattctac caacataaag aatttgcggt
      961 attgtgtgct ttttatgtaa aacaagattt ggtttataaa aatttagatc aataccatta
     1021 gacactactt ctactggtgt atcgcaacca ttttctatta attctttttt tatactattg
     1081 gaaggagcaa caacaagttc acaataattg ttaagaaaat taattaaata tgcaccatat
     1141 aattttttga ctgttttttt accaaatttt acaaaatatg atggaaaaga taaattagca
     1201 tgctgagtaa acacaactgg aatattgtgc ctttgggcat atatagctaa agcaaaacta
     1261 ataaaagcac catcgtgaat atgaattata tctggcttga tttttttaat gatatcatag
     1321 gctctaaagg aagaaaaata gcttgctcta atattttctt tgaacggaac tttaaaagat
     1381 ggcaatctaa tgatagtata atttttacct cgggattctc ggctaaattt attgttttct
     1441 gatgaagtga ttacaaaaac ttcattgctt tggtctaggg aaaaataatt agcaagatct
     1501 gaaacaatat ttgtaacgcc agaaatgtga ggtaaaaaaa cttctaaacc aaaaataatt
     1561 ttcattttaa aaatttatta tattaactga cctatgactt ctactatttg tggacccagc
     1621 gagaatcgaa ctcgcgattg tatgttgccc cgacaatttt agcaaaactt tgtggaccta
     1681 gggagaattg aactcccgtc tctacaatgc gaatgtagta ttctaccgtt aaactatagg
     1741 cccaatagaa tattaccata ttctttaaaa aattactata actcccttgt aaatttaata
     1801 ttaaaaaacc aaggaataaa attgagaaca tttttagcta caatgacatt ctcgcgttct
     1861 taaatatata agaatgtcaa taatttctga ttatttttgt tcgttctaat aatatttttg
     1921 tatctttcga attatacaat tatacaattt agtatttgtt ttgggattaa aaaacagccc
     1981 gaggcatagc ctggggttta tattttatag atgttaatgt aattgacaat ttcactgggc
     2041 acaaagtttt taatattttc attttttcta attttttctc ttattagtgt gctagaaata
     2101 gggctcatta cttttatcct ttttagattt tttattaaat tttgaatttg gggagaaaaa
     2161 gaagataaaa tatatttttt cttatagcct tggcgagtgg aaacaataaa ttttatttgc
     2221 gacagcaggg taataatgcc tttttcgccc ctttttcatc tgcctgataa aattgaatct
     2281 aaactatctt cgccaataat tcaataaatt gtcgcattgg gataaagttg ttgcaattct
     2341 ttataagtat ccagggtata agatttttta ccatggcgag cgcgttttat ttctaaatca
     2401 gaaacttcaa aatgtttgtc atattttgct aataattcag ccatctttac acgatgtttc
     2461 aagggtgcta aatctttttt tctaatcgga ggaatagcgc aaaccatcaa aagcactttt
     2521 gataaatgaa atttttgtaa tgctgttttg gctaaaagta aatggccaaa atgcggtgga
     2581 ttaaatgtgc tacccaaaac cccgattttt atttttcgtt ttatcgtgtt ttgtgtgcct
     2641 agcatattaa aattttaatc attttgaatt ctttcttgaa ttctttaaaa caaactaatg
     2701 aaccaataat aaagcaacta gttgttgcat caaggaaatt aatttgtttt gtaattcaat
     2761 gatttttgct tgacgatcaa caagatctat taaacttacg tttgtatttt gaatcttgcc
     2821 aatattagta ttggaagatg ttgaggagtt tgacgagctt gtagaagaaa cattgctaga
     2881 attgccaaca ctaatacctg tggtttcggc ttttcatttg aaaatcgaac gcgtagaata
     2941 atttttatct tgtctggata actgtggaat aataaccaac cctataacct ctttattaaa
     3001 atcaggaaca acaatgattt gagagttgct tttttgaaaa tctaaaaaga acaattgttt
     3061 taaaccattt ttataaataa taatataagg aattgtttta attaaattat cgggtggatt
     3121 ttcaaaacta aaatttaaat tgccactccc gcctgaaatt ttttgatatt tagccatata
     3181 aggtattaag gtgtcagaaa tagccaggga gctattgcca ttcattggca aatagaaact
     3241 ggaaggggca atagatatat tttttaaatt agcatcttga taacaatatt
//
LOCUS       DAOTJA010000061         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_25312_length_3162_cov_3.653042, whole genome shotgun sequence.
ACCESSION   DAOTJA010000061 DAOTJA010000000
VERSION     DAOTJA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25312_length_3162_cov_3.653042"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="argC"
                     /locus_tag="P5241_03775"
     CDS             complement(<1..735)
                     /gene="argC"
                     /locus_tag="P5241_03775"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRV32616.1"
                     /translation="MINVALIGGSGYSGKELIRLLLQHPEVKIKSIFAKSTAGKNIND
                     IYPFFKKRIDILIDSFDISKIQDIDLIFAALPHGESMEILPKLIGTNVKVIDLGGDFR
                     FQDVSVYEKWYNKEHTAKDVVKSFVYGLPELFRDKIKKANYIANPGCYPTSAILALAP
                     IVKTDYFDLNTIVINSLSGVSGAGRKATVDYSFCEVNESVKAYKIGNHQHSPEIESVL
                     ENFSGKGIDLIFVPHLIPVNRGIYTTI"
     gene            complement(826..1752)
                     /locus_tag="P5241_03780"
     CDS             complement(826..1752)
                     /locus_tag="P5241_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRV32617.1"
                     /translation="MVLTITTNPMLDKTLFLPKFEIGNIYRSTKVEDVAGGKGINVSL
                     QLTELGVDNIATGFLGGEIGLQIKHILDNDNIKNDFVWVKGNTRIGFTVLDESNLVQT
                     SVFEPNHKVSEDEKNKLSEKVNKYSSLCNYIAISGSVPDKSISGIYYDMINLIKNNNN
                     AKIFFDSYGEEFKIGINAKPFFVKQNKKEAELFFNKPLIDEKDFLQALDSYIDIHFAI
                     LTNGVNYSYIKFENDFYRVKPPKVNVINPIGSGDTMTAGLIYGFIKNYDFIDIIRFAF
                     AAAASNASKWVIARNKYEDICSYMPLIILEKL"
     gene            2011..2988
                     /locus_tag="P5241_03785"
     CDS             2011..2988
                     /locus_tag="P5241_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRV32618.1"
                     /translation="MLKKILYFLAFISLIATTYSQVIISGKISDSNNSGINMVKVYFI
                     SDRLDSVYTNSNGEYSFTAIQGKTYIIKPFKSTPTTEKRFFLPEQIVVSNIQNSINNI
                     NFTGYIFYRILCTIRDEKNETIQNINVRLTGNKNDEKLTTINGQVVFDTLVRGTYIVQ
                     PLSFIYQFTPEKYSFNDLTNDQIKYFTAKKMPTYNISGYVKDSYNNPIQNATINLEGL
                     LQKSTSTDENGYYFFTDIISGDYQISISKASYFFQENSKSVKVISSSINNINFTGLKF
                     YKISGYTYNDKNEILKNVKLEITGTKNGNTFSDLYGYYSSTRFILPHLQ"
     gene            complement(2966..>3162)
                     /locus_tag="P5241_03790"
     CDS             complement(2966..>3162)
                     /locus_tag="P5241_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32619.1"
                     /translation="CRVTFTFFKGDPFLSTTFPKIENKFNLVPQKFRLVVSFEILSIV
                     ICSGTNLYEYNKGFIVNGEE"
BASE COUNT         1225 a          483 c          374 g         1080 t
ORIGIN      
        1 tattgttgta taaatacccc tattaacagg aattaaatga ggaacaaaaa ttaaatctat
       61 tcccttacct gaaaaattct ccaaaacact ttcaatctct ggtgagtgtt gatggtttcc
      121 aattttatag gctttaacag attcattcac ctcacaaaaa ctataatcaa cggttgcttt
      181 tcttccagca cctgagacac cagataaaga attaattaca atagtattta aatcgaaata
      241 atcagttttt actattggag ctaaagctaa tattgcgcta gttgggtaac aaccaggatt
      301 tgcgatataa ttggcttttt taattttatc tctaaataat tctggtaaac cataaacgaa
      361 acttttgaca acatcttttg ccgtgtgctc cttgttatac cacttttcat aaacagaaac
      421 atcttgaaat ctaaaatcac cacccagatc aattacttta acattggtgc caattagttt
      481 tggcaatatc tccatagatt ccccatgcgg taaagctgca aaaattaaat caatgtcttg
      541 tattttgcta atatcaaatg aatcaatcag aatatcaatt ctctttttga aaaaaggata
      601 aatatcatta atatttttac cagctgtact tttagcaaaa attgatttaa ttttgacttc
      661 agggtgctgt aacaataatc ttatcaactc tttacctgaa taaccagagc caccaattaa
      721 agcgacatta atcataccaa tccttataaa attaaaatgt gcataattat acaaaatatt
      781 ttataattat gcaagttaaa aatagttaat gattagaata tcgctctaca atttttcaag
      841 aattatgaga ggcatatatg aacatatgtc ttcatattta tttcttgcta tcacccattt
      901 agaagcattt gaggcggcag ctgcgaaagc aaatcttata atatcaataa aatcataatt
      961 cttaataaaa ccataaatta aaccagcggt cattgtatca ccacttccta tggggttgat
     1021 aacatttact tttggaggtt ttactctata aaaatcgttc tcaaatttta tatacgaata
     1081 attcacacca ttcgttaata tagcaaaatg aatatcaata taactatcca gggcttgtaa
     1141 aaaatctttt tcatcaatta agggtttgtt aaaaaataat tctgcctctt ttttattttg
     1201 cttaacgaaa aaaggttttg cattaattcc tattttaaat tcctctccat aggaatcaaa
     1261 aaagatttta gcattattat tatttttaat taaattaatc atatcataat aaataccaga
     1321 aatactttta tctggtactg aacccgaaat agctatatag ttacaaagag atgaatattt
     1381 atttactttt tctgataatt tatttttttc atcttcagaa actttatggt ttggttcaaa
     1441 aactgaagtt tgaacaagat ttgactcatc taaaacagta aaaccaattc gggtattacc
     1501 cttaacccac acgaaatcgt ttttgatatt atcattatca agtatgtgtt taatctgcaa
     1561 accaatttct ccacctaaaa aaccagttgc gatattatca actcccaatt ctgtgagttg
     1621 taaagagaca tttataccct taccacctgc tacatcttct acttttgtag atcgatagat
     1681 attacctatt tcaaattttg gtaaaaataa ggttttatcc aacattggat ttgttgttat
     1741 tgttaaaacc ataagttctc aaatatttta ataaattata attaaatata aaaaattata
     1801 aaacaatatg aaagcttatt actactaaaa aaaattaaat tccaacaaag ggaaaccaat
     1861 taaaataatt cttgataatt ctaaaataaa aaactatatt tgacttaaag cgaattttgc
     1921 taagataaga aatataaaga acagacaacg tataagccca ctaagaaacg taatctaaca
     1981 atttttttta tcaacttttt aactattaat atgttaaaaa aaatattata ttttttagcg
     2041 ttcattagct taatagcaac aacttactct caagtaataa tatctgggaa aatatccgac
     2101 agtaataatt ctggtattaa tatggttaaa gtatatttta tttcagatag actagattct
     2161 gtatatacaa atagtaacgg agaatattct tttacagcta tacaaggcaa aacatacata
     2221 ataaagcctt ttaaaagcac ccctacaact gaaaaaagat tttttttacc cgagcagatt
     2281 gtggtatcga atatacaaaa ctccattaac aacataaatt ttacaggtta tattttttat
     2341 agaattttgt gtactattcg tgacgagaaa aacgaaacca ttcagaatat aaacgtccga
     2401 ttaaccggga acaaaaacga tgaaaagtta acaacaatca atggacaggt tgtttttgat
     2461 acattagtaa gaggaacata tattgttcaa cctctttcat tcatttatca gtttacacca
     2521 gaaaaatata gttttaatga tttgactaac gatcaaataa aatattttac agcaaaaaaa
     2581 atgcccacat ataatattag cggatatgta aaagatagct acaacaatcc tattcaaaat
     2641 gcaacaatta atttagaagg tttattacaa aaaagcacat ctacagatga aaatggttat
     2701 tattttttca cagatattat tagtggtgat taccaaataa gtataagtaa agcaagttat
     2761 ttttttcaag aaaattctaa atctgtaaaa gtaatatcga gttcaattaa taatataaat
     2821 tttacagggt taaaattcta caaaataagc ggatatactt ataacgataa aaatgaaatt
     2881 cttaaaaatg taaaactaga aattacagga actaaaaacg gaaatacatt tagcgattta
     2941 tatggatact attcttcgac acgatttatt cttccccatt tacaataaaa cctttattat
     3001 actcatataa atttgtacca gaacatatca caatcgataa tatttcaaat gatacaacta
     3061 atctaaattt ttggggcact aaattaaatt tattttcaat tttaggaaag gtagtagata
     3121 aaaatggatc tcctttaaaa aatgtaaaag taactctaca tg
//
LOCUS       DAOTJA010000062         3151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_25412_length_3151_cov_3.009690, whole genome shotgun sequence.
ACCESSION   DAOTJA010000062 DAOTJA010000000
VERSION     DAOTJA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25412_length_3151_cov_3.009690"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1361)
                     /locus_tag="P5241_03795"
     CDS             complement(99..1361)
                     /locus_tag="P5241_03795"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRV32620.1"
                     /translation="MDFSYGKGSFDAVLSENDIFFICEKALNKFDVDSKRILVLIPDN
                     TRHAPIGKFFKIINEIIGKRVKKLDFLVATGTHVVMTLEQIYKFISINKIEHEKFYPD
                     IDFYCHDFSRKNNFSTIGIISAKEISEISNGLFSEDIEIPINKVVFNYDKIVIISPVV
                     PHEAMGFSGGYKYFFPGISSEEFIQTFHWIASIIGTQKIIGIKDTPSRSLIHKAAEFI
                     KIPVLCLSFVINEYNDLVCLFAGDPINSWSEAVKYSEKIHISFVNKQYKKVLAITPDI
                     YEDLWVGSKAMYKIDSIIADDGELIIYNPKIKEISFVHNNAIYKIGYHVAQYFLKQWN
                     KYKNESKLIMAHSINVKGSGTYEEGIEKPRITVTLATSINKNICEKVNLNYLDPETID
                     IDNWKKNKKEDQIVIENAGQVLYKYKKI"
     gene            complement(1386..2219)
                     /locus_tag="P5241_03800"
     CDS             complement(1386..2219)
                     /locus_tag="P5241_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV32621.1"
                     /translation="MNINKLYQFYDFSGKTIVITGGAGVLCKEIALSLIDCDANVILI
                     DKNIDMAEKVLKDAKVDTEKAIAIFGDVLKPETLQKSCDIILQKFGKIDCLINGAGGN
                     NPKATTNKDFSFFDIPEEALKFVIDLNLLGTFLPSQIFGKEIAKQNEGVILNISSMNA
                     FRPLTRIPAYSASKAAVSNFTQWLAVYFAQEFSPNIRVNAIAPGFFLTEQNRFLLIDK
                     DTGKLTERGKKIIEHTPMSRFGEPSDLIGAVLWLLSPASKFVTGIILPIDGGFSSYSG
                     V"
     gene            complement(2247..>3151)
                     /locus_tag="P5241_03805"
     CDS             complement(2247..>3151)
                     /locus_tag="P5241_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV32622.1"
                     /translation="KKERALATGLFNSGANIGAIIAPLTVPLIALYFGWQWAFIITGS
                     IGLFWLFFWFIIYESPDKHKKVSRAELEYINSDIENDNKSENERVKWITLLKYKQTWS
                     FIVGKFLTDPVWWFYLFWLPSFLNKQYGMTKTEVALPIAIVYTMTTFGSIFGGWLSGR
                     LITSGWQIYKARKISLLIFALCVVPVILAQELGKYNPWFAVLIVGLAASSHQAWSANL
                     FTTVSDMFPKKAVASVTGIGGMTGAIGGILISFCTGLILDYYKSIGNIDIGYYIMFIF
                     CGLAYILAWIIFTILSPKMEVVKI"
BASE COUNT         1148 a          534 c          376 g         1093 t
ORIGIN      
        1 catattaaaa cctcataaat tttttaaaaa cctgcggcat ttcaatgatt atttcagtat
       61 tatgccgcaa gcagagagag aaatgaaaca aaattaattc atattttttt atatttataa
      121 agaacctgtc ctgcattttc aattactatt tgatcttctt ttttattttt tttccaattg
      181 tcaatatcaa tagtttccgg atctaaataa ttcaaattta ccttttcaca aatatttttg
      241 tttattgaag tagctaaagt aaccgtaatt cttggctttt caattccttc ttcataagtt
      301 ccagaacctt taacattgat tgaatgagcc ataattaatt tagattcatt tttatactta
      361 ttccattgct ttaaaaaata ctgagcaacg tgatatccaa ttttgtagat tgcattatta
      421 tgtacaaatg aaatttcttt tatttttggg ttataaataa ttaactcacc atcatcagca
      481 ataattgaat caattttata cattgcttta ctacctaccc atagatcttc ataaatatct
      541 ggggtgatag ccaacacctt tttatattgc ttattaacaa aagagatgtg aattttttct
      601 gaatatttaa ctgcttcgga ccaggaatta attggatcac cagcgaataa acaaaccaag
      661 tcattatatt cgtttataac aaaagataaa caaagaactg gaattttaat aaattctgct
      721 gctttatgaa tgagacttct tgatggagta tctttaatac caattatttt ttgagtccca
      781 ataattgaag ctatccaatg aaaagtttgg ataaattcct cactacttat acccgggaaa
      841 aaatatttgt atccgcccga aaagcccatt gcctcatgtg gtacaactgg agagattatt
      901 acaattttat cataattaaa aactacttta ttaattggaa tctcaatatc ttcagaaaat
      961 aaaccattag aaatttcact tatttctttt gcacttataa tacctattgt agaaaaatta
     1021 ttttttcttg aaaaatcatg acaatagaaa tcaatatcag gatagaattt ttcatgttcg
     1081 attttattta ttgagataaa tttatatatt tgctccagtg tcattacaac atgagtgcct
     1141 gtagcaacta aaaaatctaa ttttttaact cttttcccaa taatttcgtt aataatttta
     1201 aaaaactttc caattggtgc atgacgtgta ttatcaggaa ttagtactaa tattctcttg
     1261 gaatcaacat cgaatttatt taatgctttt tcacaaataa aaaatatatc attttcagat
     1321 aaaacagcat cgaaagaacc tttaccataa ctaaaatcca tatactacct ctttaactaa
     1381 accttttaaa caccgctata agatgagaaa cctccatcaa taggtaatat tatacctgta
     1441 acaaactttg atgctggaga aagtaaccat aaaacagctc caattaaatc agatggttca
     1501 ccaaaacgac tcataggtgt atgctcaatt attttctttc ctcgttcagt taattttcca
     1561 gtatctttat ctattaaaag aaaacggttc tgttcagtta aaaaaaatcc tggtgcaatt
     1621 gcattaacac gaatattagg agaaaattct tgagcaaagt aaacagctag ccattgagta
     1681 aaattactaa ccgcagcttt tgatgcagaa tatgcaggta ttcttgttaa cggtctaaat
     1741 gcattcatag aagagatatt taaaatcaca ccttcatttt gttttgcaat ttcttttcca
     1801 aaaatttgag atggtaaaaa agtacctaat aagttaagat ctataacaaa tttcaaagct
     1861 tcttcaggga tatcaaaaaa cgaaaagtct ttatttgttg tagcttttgg attattaccc
     1921 ccagcaccat taataagaca atcaatttta ccaaattttt gtaaaattat atcacaagat
     1981 ttttgtaatg tttcaggttt taatacgtca ccaaaaattg caattgcctt ttcagtatca
     2041 actttagcat cctttaaaac tttttctgcc atatcaatat ttttatcaat taatataaca
     2101 ttagcatcac aatcaattaa agataaagct atctctttac aaagaactcc agccccacca
     2161 gtaattacta ttgtttttcc agagaaatca taaaattggt ataatttatt tatattcata
     2221 atccacctta acaaatttat taagaattaa attttaacta cttccatttt tggagaaaga
     2281 atagtaaaga taatccacgc aaggatatat gctaaaccac aaaaaataaa cataatgtaa
     2341 taaccaatat caatatttcc aattgatttg tagtaatcta aaataagacc agtacaaaaa
     2401 gaaatcaaaa taccaccaat tgcacctgtc atacctccaa ttcctgtaac agaagcaact
     2461 gcttttttgg gaaacatatc tgatacagta gtaaaaagat ttgctgacca tgcctgatga
     2521 gatgaagctg ctaaaccaac aatcaatact gcaaaccatg gattgtattt ccctagctct
     2581 tgagcaagaa taacagggac aacgcataat gcaaaaatta ataaagaaat ttttctagct
     2641 ttatatattt gccatcccga agtaattaat cgccctgata accaaccacc aaaaatactt
     2701 ccaaatgtag tcatagtata tacaatagct ataggtaaag caacttctgt ttttgtcata
     2761 ccatattgtt tatttagaaa agaaggtaac cagaaaaggt aaaaccacca aactggatca
     2821 gtcaaaaatt taccaacaat aaatgaccat gtttgtttat attttaataa tgtaatccat
     2881 tttactcttt cattttcact tttattatcg ttttcaatat cactatttat atattctaat
     2941 tctgctctgg atactttttt atgtttatct ggagattcat aaattataaa ccaaaaaaat
     3001 aaccaaaaaa gacctattga accagtaatt ataaaagccc attgccaccc aaaataaagt
     3061 gcaattaagg gaactgttaa aggagcaatt attgctccta tattagctcc tgagttaaaa
     3121 agccctgtag ctagtgccct ttctttcttt g
//
LOCUS       DAOTJA010000063         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_25773_length_3112_cov_2.710173, whole genome shotgun sequence.
ACCESSION   DAOTJA010000063 DAOTJA010000000
VERSION     DAOTJA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25773_length_3112_cov_2.710173"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="P5241_03810"
     CDS             complement(<1..280)
                     /locus_tag="P5241_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRV32623.1"
                     /translation="MFSNIKRVHFVGIGGIGMSGIAEILINMGFTISGSDKNLTEITD
                     RLKKLCAKIYKGHSPNNVKDVDVLVYSSAVHLDNPEIVRAQQLKIPVIR"
     gene            complement(294..1391)
                     /gene="murG"
                     /locus_tag="P5241_03815"
     CDS             complement(294..1391)
                     /gene="murG"
                     /locus_tag="P5241_03815"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRV32624.1"
                     /translation="MNKVKFIFAGGGTGGHLFPAVAIAEEIKMLLPQSEIMFIGNRNK
                     IEGKIVPQYGYEFKNININGFSRKINFSNLIFPFKLLVAILKSLFYILKFKPKAVIGT
                     GGYVSGPVLWSANLLGITTILQDHNSYPGVTTRLLAKNAKEIHLAFDDAKKYFKREDN
                     IKISGFPIRREMVKCNKEDSLKFFGCDLNKKTIFITGGSQGAKKINDALAKIVLDLVN
                     LNIQIIWQTGSLQYNEIRNKFGINESIKVLEFIGDMNKAYSACDVAITRAGATTIGEL
                     LNLEVPAILIPLPTAAEDHQMKNALSLSKHNAAIVIPEYQIDTLLKDKILTLINDENK
                     LNDLKSNLRKLQVKDSAKKIAESIIKLINNN"
     gene            complement(1394..2557)
                     /gene="ftsW"
                     /locus_tag="P5241_03820"
     CDS             complement(1394..2557)
                     /gene="ftsW"
                     /locus_tag="P5241_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRV32625.1"
                     /translation="MKEKRNHIDIYVFVSVIVLLIFSLAAVYSASSTFSLAKTNDFSF
                     FFRLHLIKMIIGLFLFFIAIKIDYHFYKKITKKVLLLAILSLIILFFVGNVIKGANRW
                     IGVGLLSFQPSEFAKFALIFHLAFLLEQKEEFIHDFNKGFLPLLVWIVSVTAFVMIQP
                     NFSTGAMIFVISFIMLFIGKVRIKHILGVALSLIPLLVVYAISAEYRLKRILAFLGSS
                     ESVEEVNYQLQQAIIGFGNGGIFGVGPGQSIQRDFFLPESYGDFIFAIIGEEYGFIGA
                     VVIIFIFILIMIRGIKIAKHSIDLFGKYLAVGITSTISVYAIVNTGVACGLFPTTGLP
                     LPFLSYGGSSMIFTAFATGVLLNISSHTDIQPRTENIVEDDIQEEPIVGQVFK"
     gene            complement(2557..>3112)
                     /locus_tag="P5241_03825"
     CDS             complement(2557..>3112)
                     /locus_tag="P5241_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRV32626.1"
                     /translation="IIDTNKISIKGKHNLYNSMAASLAGFISGIKVEEIVNTLMNFEG
                     VEHRLEFVKEINGVKFYNDSKATNVDSVWYALDSFVEPINLIAGGKDKGNDYSSISEL
                     VEKKVKNLIVIGIGAKKLFDYFKEKTNVVFADTMEDAVKKAYELAKPGEIVLLSPACA
                     SFDMFNNYEHRGQVFKQCVYNLVK"
BASE COUNT         1114 a          550 c          339 g         1109 t
ORIGIN      
        1 tacgaattac tggtattttt aattgttgag ctctaacaat ctcaggatta tctaaatgaa
       61 cagcagaaga atataccaat acatctacat cttttacatt atttggtgaa tgtcctttat
      121 aaattttcgc acaaagtttt tttaatctat cagttatctc agttaaattt ttatccgatc
      181 cgcttatagt aaaacccatg tttatcagta tttctgcaat gccactcatt ccaattccgc
      241 cgattccaac aaaatgtact ctttttatat tgctaaacat aaaactctca attttaattg
      301 ttatttatta attttattat actttctgca attttttttg cactatcttt aacttgtaat
      361 tttctcaaat ttgattttaa atcattcaat ttattttcat cgtttataag tgtcaaaatt
      421 ttatctttta ataacgtatc aatttggtat tctggtatta ctatggcagc attatgtttt
      481 gataaagaca aagcattttt catttgatga tcttcagctg cagttggtaa aggaattaaa
      541 attgctggta cctccaaatt taaaagctct ccaatagtag tagctccagc tcttgtgatt
      601 gctacatcac aagcagaata agctttattc atatcgccaa taaattccag aactttaata
      661 gattcattta taccaaattt atttcttatt tcattatatt gcaaagatcc tgtttgccag
      721 attatttgta tatttaaatt aactaaatct aaaactattt tagctaatgc atcatttatt
      781 ttttttgctc cttgtgaccc accggtaata aagattgtct ttttatttaa atcacaacca
      841 aaaaatttta atgaatcctc tttattacat ttaaccattt ctcttcttat tgggaatcct
      901 gagatcttaa tgttatcctc ccttttgaag tatttttttg catcatcgaa tgctaaatga
      961 atttcttttg catttttagc gagcaatctt gtcgttactc cgggataact attgtgatcc
     1021 tgtaaaattg ttgtaatgcc tagtaaattt gctgaccata atacaggccc actaacataa
     1081 ccccctgtac caattactgc cttcggttta aattttaata tataaaacaa cgattttaat
     1141 attgctacta acaacttaaa tggaaaaatt aagttactaa aattaatttt tcttgaaaaa
     1201 ccattaatgt taatattttt aaattcataa ccgtattgtg gaacaatttt accttcaatt
     1261 ttatttctat taccaataaa cattatttct gattgaggta ataacatttt aatctcttca
     1321 gcaattgcta cagcaggaaa caaatgccca cctgtaccac caccagcaaa tataaatttt
     1381 actttattca tttttattta aatacctgtc caacaattgg ttcttcttga atatcatctt
     1441 ctacaatatt ttctgttctt ggttgaatat ctgtatgcga agaaatattc aacaaaacac
     1501 ctgtagcaaa agcagtaaat atcatagaag aacccccata acttaaaaaa ggtaaaggca
     1561 atccagtagt tgggaataaa ccacaagcta caccagtatt tactatagca taaacagaga
     1621 ttgtagaagt tattccaact gctagatact tcccaaataa atcaatagaa tgttttgcaa
     1681 tctttattcc acgaatcata ataagaataa aaataaatat tattacgacc gcaccaataa
     1741 aaccatattc ttcgccaatt atagcaaaaa taaaatctcc ataagattca ggtaaaaaga
     1801 aatccctttg aatactttga ccagggccta cgccaaaaat tccaccgtta ccgaaaccaa
     1861 ttattgcctg ttgaagctga tagtttactt cttcaacaga ctcagagcta cccaaaaaag
     1921 ctaaaattct cttcaatcta tattcagcac ttatagcata aacgactaat aaaggaatta
     1981 gagataatgc gacacctaaa atatgtttta ttcttacttt gccaataaat aacattataa
     2041 aagaaataac aaaaatcata gctccggtac tgaagttagg ttgaatcatt acaaaagcag
     2101 taactgaaac tatccatact aataatggta aaaatccttt gttaaaatca tgaataaatt
     2161 cttctttttg ttctaacaaa aaagcaagat gaaaaattaa agcaaatttt gcaaattcag
     2221 aaggttgaaa actaagtaac cctacaccaa tccatctatt tgctcctttt ataacattac
     2281 ccacaaaaaa taaaataatc agactgagta ttgctaatag aagaactttc ttcgttatct
     2341 ttttataaaa atgataatca attttaatag ctataaaaaa taagaataat cctattatca
     2401 ttttgattaa atgtaaccta aagaaaaaac taaaatcatt tgttttagca agtgaaaatg
     2461 ttgagctagc actatataca gctgctaagc taaaaatcaa taaaacaata acacttacaa
     2521 aaacataaat atcaatatga tttctttttt ctttcattat tttaccaaat tataaacaca
     2581 ttgtttaaaa acctgaccac gatgctcata attattgaac atatcaaaac tagcacaagc
     2641 tggggaaaga agtactattt caccaggttt tgccaactca taagcttttt ttacagcgtc
     2701 ttccatcgta tcggcaaata ctacatttgt tttctcttta aaataatcaa ataatttttt
     2761 agcacctata ccgataacaa ttaaattttt aacttttttc tctactaatt cactaattga
     2821 agagtagtca ttacccttat ctttgccacc agcaattaag tttattggtt caacaaagct
     2881 atctaaagca taccaaacag aatcaacatt agtcgctttt gaatcattgt aaaacttaac
     2941 accatttatt tctttaacaa actctaatct atgttcaaca ccttcaaagt tcattaatgt
     3001 attcacgatc tcttcaactt ttattccaga aataaatcca gctaaagatg ctgccataga
     3061 attatataaa ttatgtttac cttttattga tattttatta gtatcgatta tt
//
LOCUS       DAOTJA010000064         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_26691_length_3026_cov_5.564456, whole genome shotgun sequence.
ACCESSION   DAOTJA010000064 DAOTJA010000000
VERSION     DAOTJA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26691_length_3026_cov_5.564456"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    174..273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <274..569
                     /locus_tag="P5241_03830"
     CDS             <274..569
                     /locus_tag="P5241_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32627.1"
                     /translation="YNCSFQLGIFFFFSGYLFHISNKQKSDLGEQKCSDFKIFLISRI
                     KGILVPYLSLSLISMIFYLFYYHVPFYSIITFKDMLSVFLFGARNTIFYNIPL"
     gene            1628..>3026
                     /locus_tag="P5241_03835"
     CDS             1628..>3026
                     /locus_tag="P5241_03835"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRV32628.1"
                     /translation="MIAEVKKDSINTSVSGTGQVAALQQIDLKLKASGDVVYIGVKNN
                     QEVPAGTLILQLDSTDAQQAVKDAERSVENSKLDLQKAQQSSSNIQNIENQIKETGDK
                     GLKEIAQTYTDLFTILNSVDDILFERDLSSDINTNNIQYYSDVVDTFYGEHFTSVPQD
                     LANAYPEAKRLYEQGFLSYQKALHGANSEDIYKALQDAYKFTEKLANITKTARDVVDL
                     FNTSSVLENWRPDNSVLINQHLTNLRNYYNTINTHLTALLDIINSLDSLNNNLSVQSI
                     NINSLELTIQQKEDALAEAKRNLSDYYVYAPFSGIVANFDIKKGESLSNGTVIASILT
                     KQKIAEITLNEVDITNVKIGQKADITFDVIEDLHIPGEVVDIDTLATVTQGVVNYGVK
                     VVFDTQDERVKPGMSVSVSIVTNSKTNILVIPNSAIKEQNNKTYVEKVIFVGTTPKII
                     RDTITVPASKVQITMT"
BASE COUNT         1017 a          365 c          412 g         1132 t
ORIGIN      
        1 tattttaact catatgccga ttaatagaaa tctgactgcc tataattgtt cttttgctta
       61 ttaaatttag gaaatggaaa atcatcacag acttatttga gtagatattg ctcggggtat
      121 aggaataatt tttattattt taactcatat gccgattaat agaaatctga ctgnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctataa ttgttctttt caattaggta
      301 tttttttctt tttttctggt tatttgtttc atatttctaa caaacaaaaa tccgatttag
      361 gtgaacaaaa atgttcagat tttaaaatat ttttgatatc tcgaattaaa ggcattcttg
      421 tgccttattt atcattatct ttgatttcaa tgatttttta tttattttat tatcacgtac
      481 ctttttatag tattattact tttaaagata tgttatcagt ttttcttttt ggtgcaagaa
      541 atactatttt ctataatatt cccttatgat ttttgcctac attatttttg gtagaatgtt
      601 tgtattattt tttacgaaaa tattgtaata aagatataat gatactattc atctcattat
      661 tattaagctg ttttagtttt atatggctca atgctcttta ccaaacaaaa gtattttgaa
      721 cctgaccagt agctatatat tatttaattt attttactat tggcaattta gcaagaaaat
      781 acatcaagga aaataccaga attagcatat ccaatatcaa tggtattttg tttgttttgt
      841 gtttattatt aaatatctct atgtattatt ttcctgatat ttatttttct attttaagtt
      901 ttcaaaccat atcattacat agcattaagc tatattttac cgctttgcta ataatgttag
      961 caggtactta tgtgtatatt tgtatttctc gaattctggc taataaattt aaacatattt
     1021 attttttaga atatctcggt caaaattctt taatcttttt tggattacac attccagtta
     1081 tgtgatgcat tagatcgctt ttccaatatt tcgatatctc tattactgcc cgttttaatt
     1141 taattggtat tgtttacgcc tttcttacta ttttgatact ttgacccatt agttatttga
     1201 tcaataaata ttgtccctga attattggaa aatattctaa taataaatct aataatacaa
     1261 aagttttggc ataatttttt tataaaatat tatagtaaat ccctaaaagt tttattttta
     1321 tagcgattct attatattta taacatatga tgtaatatat gtaatagttg gtttatctaa
     1381 aaaataatat cctgagtatt gatttaattc attttttatg ttagtatacc tgttgctata
     1441 ttatagtaaa cttaatttta ataagaaatt taaacttaat attatgctta aaaaactaat
     1501 tccacccaag atgtttcaac aattactaaa gtataaatga ttgatattgg taattgttat
     1561 ttttattgtt ggttttggta tttttagttg aagaaatgct attagtaaca acaattcatt
     1621 aaaatatgtg atagcagaag tcaaaaaaga ctctattaat acgagcgttt cagggactgg
     1681 gcaagtagct gctttgcaac aaatagatct gaaattaaaa gcatcaggcg atgtagttta
     1741 tattggtgtt aaaaataatc aagaagtgcc agctggtacg ttaatattac aattagatag
     1801 cactgatgcc caacaggccg ttaaagacgc tgaaagaagt gtagaaaata gtaaacttga
     1861 tttacaaaaa gctcagcaat cttcaagtaa tattcaaaat attgagaatc aaattaaaga
     1921 aactggcgat aaaggattaa aagaaattgc ccagacctat acagatcttt ttactatttt
     1981 aaattctgta gatgatattt tatttgagag agatcttagt tctgatatta atactaataa
     2041 tattcaatat tatagtgatg tagttgatac gttttatggc gaacacttta catcagttcc
     2101 tcaagactta gctaatgctt atcctgaagc aaaaagatta tatgaacaag gatttttgag
     2161 ttaccagaaa gctttacatg gtgctaatag tgaagatatt tataaagccc ttcaagatgc
     2221 ttataaattt actgaaaaac ttgctaatat tactaaaact gctcgtgatg tcgtggattt
     2281 atttaatact agctctgtat tggaaaattg gagacctgat aattcagtac tcataaacca
     2341 acatttaaca aatttaagaa attattataa tacaatcaat actcacttaa ctgctttgtt
     2401 agatataata aacagcttag atagtttaaa taataatctt tctgttcaat ctataaatat
     2461 taattcttta gaacttacta ttcaacaaaa agaagatgcg ttagcagagg caaaaagaaa
     2521 tttaagtgat tattatgttt atgctccatt tagtggtatt gtagcgaatt ttgatatcaa
     2581 aaaaggagaa tctttatcta atggtacagt tatcgcatct atacttacta aacaaaaaat
     2641 agcagaaata actttaaatg aagttgatat tactaatgta aaaatagggc aaaaagctga
     2701 tattactttt gatgttattg aagatttaca tattccgggt gaagttgttg atattgatac
     2761 tttggctaca gtgacccaag gtgtagtaaa ttatggtgtt aaggttgtat ttgatacaca
     2821 agatgaaagg gtaaaacccg ggatgagtgt ttctgtttcc atagttacta atagtaagac
     2881 aaatatttta gttattccga attctgcaat taaagaacaa aataataaaa catatgtgga
     2941 aaaagtaata tttgtgggta caacacccaa aattattcga gatactataa ctgtgccagc
     3001 ttctaaagtg caaattacaa tgacct
//
LOCUS       DAOTJA010000065         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_27187_length_2976_cov_6.046902, whole genome shotgun sequence.
ACCESSION   DAOTJA010000065 DAOTJA010000000
VERSION     DAOTJA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27187_length_2976_cov_6.046902"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..271)
                     /locus_tag="P5241_03840"
     CDS             complement(65..271)
                     /locus_tag="P5241_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32629.1"
                     /translation="MPPLPLEAQTQNILQLPKFNIQAPIQISSSSDLQEIYKLLRQGV
                     VLFPGSTIPGGEYAIILGHSSSYP"
     gene            complement(556..684)
                     /locus_tag="P5241_03845"
     CDS             complement(556..684)
                     /locus_tag="P5241_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32630.1"
                     /translation="MDYGIKKNLYARNAILGAMGAGFLIIQPTILFGNTIVPIILK"
     gene            complement(808..1317)
                     /locus_tag="P5241_03850"
     CDS             complement(808..1317)
                     /locus_tag="P5241_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32631.1"
                     /translation="MNSPLHRENILNRNYTETGIAVLTATIASTGERRTIIVQEFGNQ
                     FKNKNIVANKPISSNIKSENNTSPTTIKTQPTISSTTTATITTTTTSANTTTTTAPQP
                     VTSTSIIETEVLGQTQTNNLSLPPTAIRTPEEINIKKQTPISEKTRQELAQIEFKHAI
                     ASNHVTSDD"
     gene            complement(1411..1725)
                     /locus_tag="P5241_03855"
     CDS             complement(1411..1725)
                     /locus_tag="P5241_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRV32632.1"
                     /translation="MKTQQTKKTNIPLFLRRGFLAFVVFCFIFSRFIMGLNVLVLKNP
                     HLFADITHQDILVMINDIRQKNNLPILVENPKLDQAAYLKAQDMIDKNYFSHYSPQGI
                     SP"
     gene            complement(1844..2665)
                     /locus_tag="P5241_03860"
     CDS             complement(1844..2665)
                     /locus_tag="P5241_03860"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRV32633.1"
                     /translation="MVRKIRNFLKTRKEKIIFGLCLGISILIGAFFILRETQFLPQNA
                     IVLLENVVLPGQNDTVLIFAPHQDDETLGAGGYIIRAVENKANVYIVLITNGNSRNLE
                     TKRNIEFQKITKFLGVPNSHLLYLNLPDGQLSKQDLFMIQQDITHTIKQVDPDIIIYP
                     IPQDRHIDHQISGKIIEHTLGYFPQIKSYAYLVHYPGFPRPEGLHRNAFILPPLSMLS
                     INKRWETLNLTLDEENKKCAAIYLYRTQFQNPLPFLRNLMVAFCRQNEIFLTNRE"
     gene            complement(2670..>2976)
                     /locus_tag="P5241_03865"
     CDS             complement(2670..>2976)
                     /locus_tag="P5241_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV32634.1"
                     /translation="KHKNEQNILGLNQNFDIVDDKETSTEEQIVNKETVSEIREQISK
                     LPKKYQEVVMLADIKGLDCKEIADLLNIPIGTVKSRLHRGQAMLQERMQNKNNRSIK"
BASE COUNT          966 a          447 c          462 g         1101 t
ORIGIN      
        1 aaatcaaatc cccaggtgca aattcgttta ataaagaaaa aactgattta tatcttccag
       61 gcgttcaagg atatgaagag ctatgcccca aaataatagc atattcccct cctggtattg
      121 tagatccagg aaacaataca acaccttgac gtaataattt atatatttct tgcaaatctg
      181 atgacgatga tatctgtatt ggcgcttgaa tattaaattt tggtaattgt aaaatatttt
      241 gagtttgagc ttcaagaggt aatggtggca atggtatgct tggttctgta ttaatattat
      301 tatgactatt agatgaagta ttttgtatta catttgcatt cgtgggacta ttaatgtttg
      361 tattattata tgataaaaaa tttcaattta taggacgatg catttcgttg gtaaaagata
      421 tcaccaactg atgatattta tattttgctc aggctataaa agcatcgcca ttaaaaatag
      481 cataaattag tataaatact ccaataaaaa ttcaaactaa agttgtttta aacttcatac
      541 ttttatttta attctctatt ttaatattat tggaactatt gtattaccaa ataatattgt
      601 tggctgaata attaaaaatc ctgcacccat agcacctaaa atagcattac gggcatacaa
      661 attctttttt ataccataat ccattttatc agtttttctg acaataaaca accccatagc
      721 gcttaaacat attaaaataa ttccggaact attgtttaat cattggaatg ttttggtatt
      781 aattaaagaa gtggcatgat attttaatca gtcatcagaa gttacatgat tggatgctat
      841 ggcatgctta aattctattt gcgctagttc ttgacgagtt ttttcagaaa taggagtttg
      901 ctttttgata ttaatttctt ctggtgtgcg aatagcagtt gggggcaaag aaagattgtt
      961 agtttgagtt tgtcccaaaa cttcggtttc tataatagat gtactggtaa caggctgggg
     1021 agcggtggtt gtagttgtat tagccgatgt ggttgtggta gtaatagtcg ccgtagttgt
     1081 ggaactaatt gttggctgtg ttttgatagt agtaggagat gtattatttt ctgattttat
     1141 attagaactt attggtttat tagcaacaat attcttgttt ttaaattggt taccaaattc
     1201 ttgaactata atagtccttc gctcgcccgt agacgctatg gttgctgtta aaacagctat
     1261 accagtttcg gtataatttc tatttaaaat attttcgcgg tgtagaggag aattcattca
     1321 agcattaaaa gcttccgtgg cattataaaa attcattgct aaattttcac cggcatattg
     1381 ataataatat cccacttgat caaatcaata tcaaggggaa ataccctggg ggctatagtg
     1441 cgaaaaataa tttttgtcta tcatatcttg tgcctttaaa taagcagcct gatcaagttt
     1501 gggattttct accaaaattg gtaaattatt tttttgacga atatcattaa tcataactaa
     1561 tatatcttga tgagtaatgt ccgcaaataa atgagggttt tttaatacta aaacattcaa
     1621 ccccataata aaacgagaaa aaataaaaca aaaaaccaca aaagctaaaa aacccctgcg
     1681 taaaaataaa ggaatatttg ttttttttgt ttgttgagtt ttcattttat tttttgtttt
     1741 aaaatattta attatatgta aaaatatatt taattatatg taaaaatccc taaaaatcta
     1801 taaaaaccca catttttatt gttctaatat atattatacc atattattct ctatttgtca
     1861 agaaaatctc attttgacga caaaaggcaa ccattaaatt tcgtaaaaat ggtaacgggt
     1921 tttgaaattg agttctatac aaatatatag cagcacattt tttgttctct tcatctaggg
     1981 ttaaatttaa agtttcccat cttttattaa tactcaacat tgaaagaggg ggcaatataa
     2041 aagcattcct atgcaacccc tcgggacgag gaaaccctgg ataatgaact aaataagcat
     2101 aactttttat ttgaggaaaa tatcctaaag tatgctcgat gattttgccg cttatttgat
     2161 gatctatatg tctatcttgg ggtattggat aaattattat atctggatcg acctgtttta
     2221 ttgtatgagt aatatcctgc tgtatcataa atagatcttg tttagacaat tgcccgtctg
     2281 gtaaatttag atataataaa tgtgaatttg gtacacccaa aaattttgtt atcttctgaa
     2341 attctatatt acgctttgtt tctaaattac gactgttgcc attagttatt aaaactatat
     2401 aaacattggc tttattttct actgctcgaa taatataccc gcccgcgccg agtgtttcat
     2461 catcttgatg aggagcaaaa attaaaactg tatcattttg tccaggaaga acaacatttt
     2521 ccaataatac tatagcgttt tggggtaaaa actgggtttc gcgcaaaata aaaaacgcgc
     2581 cgatcaaaat agatataccc aaacacaatc caaagataat tttttctttt cgtgttttta
     2641 aaaaattgcg aatctttcta accataaaac tatttgatac ttcgattatt cttgttttgc
     2701 attcgctctt gtaacattgc ctgtcctcta tgtaaacgtg atttaactgt ccctatagga
     2761 atattcaaca gatcagcaat ttctttacag tctaacccct taatatcggc aagcattaca
     2821 acttcctgat atttttttgg taattttgat atttgttctc ttatctcgga aaccgtttct
     2881 ttgtttacaa tttgttcttc cgtagatgtt tctttatcat caacaatatc aaaattttga
     2941 tttaacccta atatgttttg ctcattttta tgtttt
//
LOCUS       DAOTJA010000066         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_27557_length_2942_cov_5.114998, whole genome shotgun sequence.
ACCESSION   DAOTJA010000066 DAOTJA010000000
VERSION     DAOTJA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27557_length_2942_cov_5.114998"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="P5241_03870"
     CDS             <1..291
                     /locus_tag="P5241_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32635.1"
                     /translation="LFIGLFLIRIFELYFFLKRVSRLCYEYDWKFVSYNEEYISEILK
                     KDYYLKSEWSAYNFMFLNGPSPLKMFFSFKLLTIENIYKKDVIERIKKYANF"
     gene            278..928
                     /locus_tag="P5241_03875"
     CDS             278..928
                     /locus_tag="P5241_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32636.1"
                     /translation="MRIFEIYTKDIILRDFWNKLEESLEINEGETGLTNYLSLANEII
                     FKKFGINPSDKVYFIAGSARLYLYPELREAFGLTSQIGDLDIVIPYKKFWIQAGLEEE
                     WNNGGIYRPTNDASIEAFNVWDPARAGGAYADYKVRPTNDILRDANMINGYYFMSLAD
                     IIDYKMALNRDKEQEVVNLIKQYQGKNTMGRSEFLNKMIKLIGINNTKNFLGIVGK"
     gene            997..1072
                     /locus_tag="P5241_03880"
     tRNA            997..1072
                     /locus_tag="P5241_03880"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1032..1034,aa:Asp,seq:gtc)
     gene            1094..1166
                     /locus_tag="P5241_03885"
     tRNA            1094..1166
                     /locus_tag="P5241_03885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ala,seq:tgc)
     gene            1513..1601
                     /locus_tag="P5241_03890"
     tRNA            1513..1601
                     /locus_tag="P5241_03890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1561..1563,aa:Ser,seq:aga)
     gene            1759..1834
                     /locus_tag="P5241_03895"
     tRNA            1759..1834
                     /locus_tag="P5241_03895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1795..1797,aa:Phe,seq:gaa)
     gene            complement(2103..2837)
                     /locus_tag="P5241_03900"
     CDS             complement(2103..2837)
                     /locus_tag="P5241_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32637.1"
                     /translation="MQEHNWGLKNGNLALFDVGYGNFFEEFDVEPEEINLNEYTSSSR
                     INEFTELAEKIAKKLNLGDVKFLGMGANGYAFDTTSGKILKITSDKTEAINSKKLLGK
                     NLKHVAKIFSLNTLKKDNYNGYYIIVLEKLDTSNYDKLKNILISIYDWFDESTTLRYD
                     PDIIDNISKKHPEVAKFIYDIYKLGYEATQNKWQEKLIHDTKYNWDDISEIVPWIRGA
                     KEANTDSYYSEPPEHIYRLVKKLGGI"
BASE COUNT         1022 a          379 c          477 g         1064 t
ORIGIN      
        1 ttatttatag ggttattttt aattagaata tttgaacttt atttttttct taaaagggta
       61 agtagattat gttatgaata tgattggaag tttgttagtt ataatgaaga atatatttca
      121 gaaattctaa aaaaagatta ttatttaaaa agtgaatggt cagcatataa ttttatgttt
      181 cttaatggac ctagtccatt gaaaatgttt ttttctttta aacttctcac aattgaaaat
      241 atttataaaa aagatgtaat agaaagaatt aaaaaatatg cgaatttttg aaatttatac
      301 taaagatatt attctaagag atttttggaa taaattagaa gaaagtttag aaataaatga
      361 aggcgaaact ggtttaacga actatctttc tctagctaat gaaattatat ttaaaaaatt
      421 tgggattaat ccatcagata aagtttattt tattgcaggt tctgcaagat tgtatttata
      481 tccagaactt agagaagctt ttggtttaac aagtcaaata ggggatttag atattgttat
      541 tccttataaa aaattttgga tacaagcagg attagaagaa gaatggaata atggaggaat
      601 ttatagacca actaatgatg cttcaattga agctttcaat gtttgggacc cagctagagc
      661 tggtggggca tatgctgact ataaagttag accaaccaat gatatcctac gtgatgcaaa
      721 tatgattaat ggatattatt ttatgagctt agctgatatt atagattata aaatggcatt
      781 aaatagagat aaagaacagg aagttgtaaa tttaattaaa caatatcaag gaaaaaatac
      841 catgggaagg tctgagtttt taaataaaat gattaaatta attggtatta ataatacaaa
      901 aaatttttta ggaatagttg gtaaatagtt aaatttaaat aatttaaata atttaaattt
      961 ttaaactttt tgataatgtt ttatttaaaa aaagatggct tgtgtggtgt aattggctag
     1021 cacatttgcc tgtcacgcaa aaggtattgg ggttcgaatc ccccgcgagc cgctaaagta
     1081 attatttttt ttgggggtgt tagctcagtt ggcagagcgt ctgtcctgca agcagaaggc
     1141 catgggttcg aatcccttac actccacaat aaataaagga tgttttcagc aaataaaatt
     1201 ggttaataaa caaaattgac tttcttttaa ggttcaaagt cttcacgatg taaaactatc
     1261 acatcctgtt tcttaacaaa tataaaaaaa tataaaaaaa atttgcttaa ttaaaaaaaa
     1321 ttaagtatat ttgtattatg aagtttattt tgcaaaaaat tgctaattac attataagaa
     1381 aaatggagaa atcttcgaaa gaaaaaatag cattttggta taatgtagga atacttcttg
     1441 attcaattgc aacatcattt ggcgttgaat tggaatagtt ctgtgaatta ttattacaat
     1501 acttgtctgc acggtgaggt agcttagttg gtaaagcagt aagatacctt gcaaaaggtt
     1561 agaaaactta tgtgttgata gttcgaatct atctctcacc acaagaaatc caaccgtatt
     1621 ggatgagctg ccttaaggca gtgaccttgg aattggtcgc ctgttgctat tataaagcac
     1681 agggttcaat gtcatccatt taagcggcaa tggtaccagt tggggttttt ccacccacgg
     1741 aaacaactgg ttttttatgg tcgagtagct cagttttagg tagagcatcg gactgaaaat
     1801 ccgagtgtcg ctggttcgaa tcccgcctcg accacatatt ttaaatattt aaaaaatgtg
     1861 agtaatgagg aattgtatta aagatttaat aaagttgaat tagaatttta ctaagaaaaa
     1921 gaaatgctga taaattcctt aattataata acaatgcctt ttgtaattgc attgatgata
     1981 ttaatgttta ttggaatacc atttgttttt ttattacagg aaagtggact tacccgcttg
     2041 gcaaaaaaga atataatttt aaattggttt aggtcattac gacttattta aaaaaaaata
     2101 gtttatattc cacctaattt tttgactaat ctatatatgt gttctggtgg ctcggaataa
     2161 tatgaatcgg tgttagcttc tttagcacct ctaatccatg gtacgatttc actaatatca
     2221 tcccaattat attttgtatc gtgaattaat ttttcttgcc atttattctg agttgcttca
     2281 taacctaatt tataaatatc atatataaat tttgcaactt cggggtgttt cttgctaata
     2341 ttatcaatta tatctgggtc atatcgtaga gttgtgcttt catcgaacca atcataaatt
     2401 gaaattaaaa tattttttaa tttatcataa tttgatgtgt ctaatttttc aagaacaatg
     2461 atataataac cattataatt atctttttta agagtattaa gagaaaagat ttttgctacg
     2521 tgttttaaat tttttccaag aagttttttt gaattaatag cttcggtttt atctgatgta
     2581 atttttaata tttttccact tgttgtatca aaagcatatc cattagcacc cattcccaaa
     2641 aatttcacat ccccaaggtt taatttttta gctatttttt ctgctaattc agtaaattca
     2701 ttaattctac ttgaacttgt atattcattt aggttaatct cttctggctc aacatcgaat
     2761 tcttcaaaaa aatttccata tcctacatca aataaagcta aatttccatt tttaagaccc
     2821 caattatgtt cttgcattac atcgttttgc ggcttggcgt agtggcggat ttttagcaca
     2881 aaatttaaat cgaagaactg cacttgaacc taccacaaaa ctgtcatacg aagcactgaa
     2941 cc
//
LOCUS       DAOTJA010000067         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_27743_length_2925_cov_4.741115, whole genome shotgun sequence.
ACCESSION   DAOTJA010000067 DAOTJA010000000
VERSION     DAOTJA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27743_length_2925_cov_4.741115"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..415)
                     /locus_tag="P5241_03905"
     CDS             complement(101..415)
                     /locus_tag="P5241_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRV32638.1"
                     /translation="MILLLLFCWYFKQIIIVFIGALVLATSLEGLIQISQKIFKNRFI
                     SVLVVYVILLVLIGLLIYAIMPLLINNLIDLINDFPTLLQRPEISNIINNFISENITT
                     SQ"
     gene            360..668
                     /locus_tag="P5241_03910"
     CDS             360..668
                     /locus_tag="P5241_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32639.1"
                     /translation="MKTIIICLKYQQNNSNKIIPVILKIVFQDNVCSCDLFSIIYTYF
                     LIVYKELRTLLNGDLFFIELTMAKLALFFLNIFCAKSWISFGVIFVIFSIVCLGVIIG
                     "
     gene            complement(893..1804)
                     /locus_tag="P5241_03915"
     CDS             complement(893..1804)
                     /locus_tag="P5241_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRV32640.1"
                     /translation="MDKIIKQIPNKIEYTKYKLSNGLTIVLVPDTKLLSVLVNLYIKV
                     GSQYEPAHISGISHLLEHMSFKGTKNHPTNLELVTPLDNIGSSYNAGTGYELTQYFVR
                     CGFQYIDTALNFISDIFQNSIFPEPEFHKEKGVVLEEIHMYEDESRTKVEDDFLKLIY
                     GNQGAGRSIAGSEQTVKNITHKELLNYYRKYYNAKNAYLFIAGNFDPIQTRKLIAKYF
                     NTAKSTHSFPYPKTKILQKRPKLSLTYKDNKETHFILGFHGYPFFDETNYALHILETI
                     LSGGFTSRLFQLVREKLGGAYYIYSDN"
     gene            complement(1830..2030)
                     /locus_tag="P5241_03920"
     CDS             complement(1830..2030)
                     /locus_tag="P5241_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV32641.1"
                     /translation="MDYNIQYDETGTIGKRYRRQDEIGTPWCLTIDFDTLKDNTITIR
                     DRDTMSQKRIAVDDVGHNYFNF"
     gene            complement(2034..2222)
                     /locus_tag="P5241_03925"
     CDS             complement(2034..2222)
                     /locus_tag="P5241_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32642.1"
                     /translation="MAVLLDAYDEIKGGRSTTTNAIKDTEIVLHLHPKLAPVKCAILP
                     LLKNKIELVNKAKDIYNL"
     gene            complement(2340..2672)
                     /locus_tag="P5241_03930"
     CDS             complement(2340..2672)
                     /locus_tag="P5241_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32643.1"
                     /translation="MRLKIPFGIAQIGKAFRNEINPNNFIFRTREFEQMEMEFFVHPN
                     DSEKYFEYFKQERMQWYIKLGIKKENLRFYEHKKDELAHYAIKAFDIEYHFPFGWQEI
                     EGIHNRGQ"
     gene            complement(2685..>2925)
                     /locus_tag="P5241_03935"
     CDS             complement(2685..>2925)
                     /locus_tag="P5241_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRV32644.1"
                     /translation="SGHLSAGFADELVECKQCHKRFKLSDLDNKSVCPECQGELLPPR
                     HFNIMMKTFIGPVEDSISVTYLRGETCQGIFMDYQ"
BASE COUNT          937 a          439 c          409 g         1140 t
ORIGIN      
        1 atattagcag cattaacaaa atatctgtaa attttgagaa aaatagtcca atatttgctg
       61 tcaaatcttt tatataggtt ataatatttt gtgtatttaa tcattggctc gttgtgatat
      121 tttctgaaat aaaattgttt ataatattgc taatttcagg tcgttgtagt aaagttggaa
      181 aatcattaat aagatcaatt aaattattta ttagcaaagg cataatagca taaattagta
      241 atcctatcaa tactaataat ataacataaa ctactaatac agatataaat ctatttttga
      301 atatcttttg gcttatttgt attaatcctt ccaaacttgt tgccagtacc aatgccccta
      361 tgaagactat aataatttgt ttaaaatacc aacaaaataa cagcaacaaa atcattcctg
      421 taattctcaa aatagttttt caagacaatg tttgctcttg tgatttattt tccataattt
      481 acacttattt tttaattgtt tataaagaat taagaacttt gttaaatgga gatttgtttt
      541 ttattgaact tactatggct aaattagcat tgttttttct gaatattttt tgtgctaaaa
      601 gttggatatc ttttggtgtt atttttgtta ttttttcaat agtttgtctg ggagttatta
      661 ttgggtgatt gtacagtatt ggctgcgtta gatagtgata taagtaatat ggttgatcca
      721 tttttatgaa caattttcct ataatataat ttttggcttt ttgtagttct tgatttgtta
      781 gcggtttatc ttttagagtt tttatttctt tgataatttc tgaaaatatt agctctgctc
      841 tttgcgaatc aatcccgcct ctaattataa aaatacctct atcaaaaaat agtcaattat
      901 cgctataaat ataataagct ccaccaagtt tttcccgtac taattggaaa agacgcgaag
      961 taaaaccacc tgagagaatt gtctccaata tatgtaaagc ataatttgtt tcatcaaaga
     1021 aaggatatcc gtgaaacccc aaaataaaat gtgtttcttt attatcttta taagttaatg
     1081 ataacttggg gcgtttttgt aatattttag tttttgggta tgggaaacta tgtgtgcttt
     1141 tggcagtatt aaaatatttt gcaattagtt tacgagtttg gattgggtca aaattaccag
     1201 ctataaataa ataagcattt ttggcattgt aatatttgcg gtaataattt aataattctt
     1261 tatgagtaat atttttgaca gtttgctcac taccagcaat agatcgccct gccccttgat
     1321 tgccatatat aagttttaaa aaatcatctt ctacttttgt tcgagattca tcttcataca
     1381 tatggatttc ttccaatact acaccttttt ctttatgaaa ttctggttca gggaaaatag
     1441 aattttggaa aatatcacta ataaaattta aggctgtatc aatatattgg aatccacatc
     1501 ttacaaaata ttgagttagt tcatatccag tacctgcatt ataagaactg ccgatattat
     1561 ccaaaggcgt tacgagttct aaatttgtcg gatgattttt tgtgccctta aaactcatat
     1621 gttctaaaag atgcgatata ccagaaatat gtgccggttc atattgagaa cctactttaa
     1681 tatagagatt taccaaaaca cttaatagtt ttgtgtctgg taccaatact attgttagtc
     1741 catttgataa tttatatttt gtatattcaa ttttgtttgg aatttgttta atgattttat
     1801 ccatattaaa aatttaaaat tataaaaaat taaaaattaa aataattatg tccaacatcg
     1861 tcaacagcta tacgcttttg cgacattgtg tctctatctc gtatagttat tgtattatct
     1921 tttaatgtat caaaatctat tgtaagacac caaggtgtgc caatctcgtc ttgtcgtcta
     1981 tatctcttgc ctattgtccc tgtttcatca tattggatat tataatccaa ttttcataga
     2041 ttataaatat ctttggcttt gtttactaat tctattttat ttttgagcaa aggcaatata
     2101 gcacatttaa caggagcaag tttaggatgt aaatgtaaaa caatttctgt atcttttata
     2161 gcattagtag ttgtacttct gccacctttt atctcatcat atgcgtctaa aagtacagcc
     2221 aaagtaaccc tatctgcgcc cgctgaagtt tctataacaa aaggaataaa agtttctttt
     2281 ttctcaggat caaaatatga aagatctttg ccactaaatt ttgaatgatt gcctaagtct
     2341 cactgccctc tattatgtat accttctatt tcttgccaac caaaggggaa atgatattct
     2401 atatcaaaag cttttattgc gtagtgggct aattcgtctt ttttatgttc gtaaaatctt
     2461 agattttctt tttttatacc aagttttata taccactgca ttctttcttg tttgaaatat
     2521 tcaaaatatt tttctgagtc atttgggtga acaaaaaatt ccatttccat ttgttcaaat
     2581 tctcttgttc taaaaataaa attattagga tttatttcat tacgaaaagc tttaccaatt
     2641 tgagcaattc caaaaggaat tttaagcctc atagcatcta aaactcactg ataatccata
     2701 aaaataccct ggcatgtttc tcctcgtaga taagttacag atatactatc ttctactggg
     2761 cctataaaag ttttcatcat tatattaaag tgtcttggtg gcaataattc accttggcac
     2821 tcagggcaga ctgatttatt atccaaatcg ctcaatttaa atcgtttatg gcattgtttg
     2881 cactcgacta actcatcagc aaaaccagct gataaatggc cagaa
//
LOCUS       DAOTJA010000068         2891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_28126_length_2891_cov_7.893159, whole genome shotgun sequence.
ACCESSION   DAOTJA010000068 DAOTJA010000000
VERSION     DAOTJA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28126_length_2891_cov_7.893159"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..957
                     /locus_tag="P5241_03940"
     CDS             286..957
                     /locus_tag="P5241_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32645.1"
                     /translation="MAFYDLNGSYLNINQFIQYYEQYYFLGTPNVGRRITNRNKTNHF
                     IEKKIQNILQRGIAINDIVFIIAWKIGAIDHGLSNNRVVYKYNFNNTLQFKTRFNVID
                     VKLIVNYIINNFVTLININNPHTLFQNLFNNRGLNSNFGLVYCISLIYFFTQGQYPIY
                     DKYVHIAINAFLNNVPPNNQIGYQQINQWKDYINFINQLTRIFGNQNIGRKIDRALWV
                     YGHCF"
     gene            1176..1688
                     /locus_tag="P5241_03945"
     CDS             1176..1688
                     /locus_tag="P5241_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32646.1"
                     /translation="MKNRIQLISQTNFEMHILLKKKWSIIIFCVIWLAGWTAGGIAVI
                     IDIGTNPTPFMIIWFIVWLLGELFVLYALLWNAFGKEIIKQLNNKIVLKNDILGFGFS
                     KKYEIKKISNVRASGYFGTPDMFNLVVWNIVGGTIAFDYEGKTIRFGIALEEAEAKEA
                     ENILNNYLKK"
     gene            2329..2700
                     /locus_tag="P5241_03950"
     CDS             2329..2700
                     /locus_tag="P5241_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRV32647.1"
                     /translation="MDRGDFSNLTGFEWDDRNLNENCKKHQFTTGECEKVFFNEPLYV
                     HYDENHSLLKNRYFLPGETNEKIKLFFVFTPLKSLIRILSACDMHKNKKKVYKHLKKD
                     IPHQSLIKMYVAEKIKDDCMH"
BASE COUNT         1109 a          382 c          473 g          927 t
ORIGIN      
        1 gatacgagaa aagataaaag acaaaacgca gtaacaacag atagtggaag gttataaagt
       61 ttccatggta catgagaatt tttgtggaaa aaaatagaga gtgatttcct taagaatata
      121 ggcaataata acgaataatg aaatcactat acaatatcat gttcataata aaactaaaaa
      181 aggaattatg cgaaaatttt atataaatgg gatatatgaa atagtatata tgaagaagtt
      241 gtgtgaaact tgctgtaaaa atattttggc acaaggggta agctaatggc cttttacgat
      301 cttaatggga gctatttaaa tattaaccaa ttcatacagt attacgaaca atattatttt
      361 ttaggcacac caaacgttgg tagaagaatt acaaatagaa ataaaacaaa ccattttatt
      421 gaaaaaaaaa tacaaaatat tttgcaaagg ggtattgcta taaatgatat tgtatttatt
      481 atcgcatgga aaataggagc aatagaccat ggattatcta acaacagagt tgtttataaa
      541 tataatttca ataatactct tcaattcaaa accaggttta atgtaattga tgttaaactt
      601 atcgtaaatt atataattaa taattttgtt acgctaataa atataaataa tcctcataca
      661 ttatttcaaa atttgttcaa taatcgaggg ctaaattcta attttggtct ggtatactgc
      721 atttctttga tttacttttt tacacaagga caatacccta tttatgataa atatgtacat
      781 atagcaataa atgcttttct taataatgtt ccgcccaata accagattgg gtaccaacaa
      841 attaatcagt ggaaagacta tataaatttt ataaatcaat taactagaat ttttgggaat
      901 caaaatattg gaagaaagat tgatagagct ctctgggtat atggtcactg tttttaaaat
      961 gctccaaaca acgtgcaaca cagcttttgg aatttgtcga gcactcttac aggcttagtc
     1021 atatttttaa tctttgattt cacccagtga agacatagca taaggcgaaa acatcaagtg
     1081 caattttggg gcaaataaaa gtaaaaaaca tatgactgat tcaatgtaat attgcataac
     1141 tacagttgat gttttcaatt acataaggag cagacatgaa aaatagaata caacttatat
     1201 cgcaaacaaa ttttgaaatg catatactct taaagaagaa atggtctata attatttttt
     1261 gtgtaatatg gctggcagga tggaccgcag gtgggattgc tgtaatcatt gatataggaa
     1321 caaatccaac gccctttatg attatctggt tcattgtgtg gctattaggc gaattatttg
     1381 tattatatgc cttgctctgg aatgcattcg gtaaagaaat tattaaacag ttaaataata
     1441 agattgtttt aaaaaatgat atattgggtt ttggtttttc taaaaaatat gaaataaaaa
     1501 aaatttctaa tgttcgtgca tctggatatt ttggaacacc tgatatgttt aatcttgtag
     1561 tttggaatat cgtaggtgga acaatagctt ttgattatga aggcaaaaca ataagatttg
     1621 gtatagcttt agaagaagca gaagcaaaag aagctgaaaa catattaaac aattatttaa
     1681 aaaaataacg atggtagatt taaaagacta cctaaaacaa cacttagctt ggcgtatggt
     1741 atgttggtca aaaaaatcaa aatcatatcg tttggtattt gtttcttttt tcaatataat
     1801 atttcagtat tatgccactt gtgtttatta taccatctga agaacttgat agcaaattgg
     1861 taatatatca aaattaaaga tgacgggttc atgaagcttt acctatctat cataaaatag
     1921 ctgaaaaaga aaaaattgtt catttatttt aggtgagacg aatggatatg agtattcaga
     1981 ttatgtaagc gcatacataa ttgccatgtc tcatactact aagagttata tgaagttgcc
     2041 tgccagctat tcggcaagag ctgaacatcc gtgcgcagtt aaaaataaca aaataatatt
     2101 gaattacata aaaaaacaag atttcatttg atgtgggaaa aataatttgt aagaaaatcc
     2161 attattgaat tatataagga gttcctttaa tctttttata ccattaataa acaagatttc
     2221 cacagaagta acagataata aattgacaaa caagagacat tatgtcacaa aagaacaatg
     2281 aagcataata agaaaattca ttagtatgca tatactgtac atatacaaat ggataggggt
     2341 gacttttcaa atttaacagg ttttgaatgg gatgatagaa acctcaacga aaactgcaag
     2401 aaacatcaat ttactactgg tgaatgcgag aaagttttct ttaatgaacc attgtatgtt
     2461 cattatgacg aaaatcattc actgcttaaa aatagatatt tcctgcccgg ggaaacaaac
     2521 gaaaaaataa aattgttctt tgtatttact ccactaaaat cacttatacg aatactatca
     2581 gcatgtgata tgcataaaaa caaaaagaaa gtttataagc accttaaaaa agatattccc
     2641 catcagtctt taattaagat gtatgtagcc gagaagatta aggacgattg tatgcattaa
     2701 tgttttgcaa cagtatgtaa gcgcttgtat tcaccacgca cctgctatca gggatggatg
     2761 tgtctatggt aaaccagaat cttataaaga ggatgtattg ataattaatc gcacagcatt
     2821 ttcatcaatc aataatttat tggaaatctc ctctgagctc caacctttgt ttgatagttc
     2881 ccgtactgtt t
//
LOCUS       DAOTJA010000069         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_28970_length_2826_cov_5.674125, whole genome shotgun sequence.
ACCESSION   DAOTJA010000069 DAOTJA010000000
VERSION     DAOTJA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28970_length_2826_cov_5.674125"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..612)
                     /gene="rpsM"
                     /locus_tag="P5241_03955"
     CDS             complement(223..612)
                     /gene="rpsM"
                     /locus_tag="P5241_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRV32648.1"
                     /translation="MTRICGINIPDNKQIAYSLPYIYGIGHSLAVKILENTKVDLTKK
                     TKDLTPEEIKRIQEYIEKNFTIEGDLRRVIKKDITRLVNINSFRGARHIKHLPCHGQR
                     TKTNARTVRGRKKITVGSGRKKAATPT"
     gene            complement(676..792)
                     /gene="rpmJ"
                     /locus_tag="P5241_03960"
     CDS             complement(676..792)
                     /gene="rpmJ"
                     /locus_tag="P5241_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRV32649.1"
                     /translation="MKVKASVKKICKDCKIVRRKGRVFVICKKNPKHKQRQG"
     gene            complement(853..1077)
                     /gene="infA"
                     /locus_tag="P5241_03965"
     CDS             complement(853..1077)
                     /gene="infA"
                     /locus_tag="P5241_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRV32650.1"
                     /translation="MNSEQKNKNLIIDGVVTESLPSTTFRVKLDDGREILAYLAGKLR
                     MNFIKILVGDRVKIEMSAYDDSRGRIIYRY"
     gene            complement(1252..2745)
                     /locus_tag="P5241_03970"
     CDS             complement(1252..2745)
                     /locus_tag="P5241_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32651.1"
                     /translation="MPRSKNTISKKDEDENKKYFDIISPNETLSQDQADDQSEEQQEG
                     VIKIRKEHFATPHSKEENFEQDTNEILDKEPSKENISIPEVTSASYIERFETEKERAE
                     DMGLKASSIFQEETEIEGNYEKKGISKRFSFKIKNKVLKVSVVCFVVLGLIASLYTFV
                     FNKADIIITTKKSNIKYEGIVLADINAKAVDVSKGIIPGKLISFSKTIDKEFEATGKV
                     TGGSKAHGTMIIYNAYSQAPQVLVTGTRFESADGLIFKLDARTVVPGATLKNGEIVPV
                     SIEASVTAAEAGAAYNIAPGRFTIPGFEGTDKFKGFYGETKTKMTGGSDGESIVVSQG
                     DIKNAENVITDELFKVLNEELNKQISKDERVFKEAVVTKVGNITPNVKVGDSVAKFKV
                     SVSGEIQTIIIANKDLDELVKKNLSSALLPIDQLYGNPEYIFSNLKLDIDKGSLSFYV
                     ICDYPVTKSLNKEEIIGQIKGKSIEEVKKLLSNNEKIENTKIKLSPF"
BASE COUNT          844 a          506 c          320 g         1156 t
ORIGIN      
        1 gattcatcag ccaattgaat tattgtatta ttaaaagttg aattcacaaa aataatagcc
       61 ttttgtagtg atttagatga tttacctttt ttctccgcct tttttgctga acttgatact
      121 ttttctgctt catctgtatt tgaagatttt gttataattc tttttttacc cattgtaaat
      181 aaatttaaaa atttcctaac ttttattttt tattatctaa ttctatgtag gagttgcggc
      241 tttctttctt cctgacccca cagttatttt cttacgaccc ctaactgttc tggcatttgt
      301 ttttgttctt tgcccgtgac aaggcaaatg tttaatatgt cttgcgcctc taaatgaatt
      361 aatattcaca agccgagtaa tatctttttt tattactcta cgaagatctc cttcaattgt
      421 aaaatttttt tcaatatatt cttgaatcct tttgatttct tctggagtca aatcttttgt
      481 cttttttgta agatcaactt tggtattttc caaaattttt acagccaaag aatgccctat
      541 accatatata tatggtaaag agtaagctat ttgtttgtta tctgggatat taattccaca
      601 aattcttgtc atatattcaa atttaaagtc aagaactact ggtcaaaggc ttatagcaaa
      661 aagccattaa actttttatc cttgtctttg tttatgtttt ggattctttt tacagattac
      721 aaaaactcgg cctttacgac gaactatttt acaatcttta caaatttttt taacagatgc
      781 ctttactttc ataatacaat ttttatatta acagtatttt taaaaccaat tctattttat
      841 taaaagaaaa atttagtagc gatagattat tctgccccta ctatcatcat atgcggacat
      901 ttcaattttt actctatcgc ctactaaaat tttaataaaa ttcattctta atttaccggc
      961 caaataagcc aaaatttctc tgccatcatc taatttcaca cgaaaagttg ttgaaggcaa
     1021 cgattctgta acaacgccat caataatcaa attcttattt ttttgttctg aattcatata
     1081 tttagtattt ttaacttcga atttatatat ttttatattt tataaccagt atattcaacc
     1141 attagctatc ctaaagccta tttctttata tacctttata tactactaaa aaaacgcaaa
     1201 ttagtcaaga gttacatcaa ttttctcttt ggcagcaggc aacgcattca atcaaaacgg
     1261 agatagcttt attttggtat tttctatttt ttcattgtta gacaaaagtt ttttaacttc
     1321 ttcaatactt ttacctttaa tttgtcctat aatttcttct ttatttaaac tttttgtcac
     1381 cggataatca caaattacat aaaatgataa acttcctttg tctatatcca attttaaatt
     1441 agaaaaaata tattcaggat tgccatataa ttgatctata ggtaaaagcg cagatgataa
     1501 attctttttg accaattcat ccaaatcttt gttggctata ataatagttt gtatttctcc
     1561 tgacactgat actttaaatt tagcaacact atcaccaact ttaacattag gtgttatatt
     1621 gcctactttt gttaccacag cttccttaaa cactcgttca tctttggaaa tttgtttatt
     1681 taattcttca tttagtactt taaataattc gtctgtgata acattttctg cattcttaat
     1741 atctccctgg gaaacaacaa tagattcacc gtcactgcca ccagtcattt ttgtttttgt
     1801 ttctccataa aatcctttaa atttatcagt gccctcaaac ccaggaatag taaaacgtcc
     1861 tggtgcaata ttataagcag cacctgcttc agcagctgtc acactggctt ctatagatac
     1921 aggaacaatt tctccatttt ttaaagtagc gccaggtact actgttctag cgtctaattt
     1981 aaaaatcaat ccatcagcac tctcaaacct tgtacctgtt accaaaactt gtggcgcttg
     2041 actataagca ttataaataa tcattgtgcc atgagctttg gaacctccgg tgactttacc
     2101 agtagcctca aattctttat ctatagtttt ggaaaatgaa attaatttac ccggtataat
     2161 acctttggat acatccacag ctttggcatt tatatctgcc aaaactattc cttcatattt
     2221 tatattggat tttttagtcg taattataat atctgcctta ttaaaaacaa atgtatacaa
     2281 actcgcaatc aggcctaata ccacaaaaca aacaacagat acttttaata ctttattttt
     2341 gattttaaaa ctaaatcttt tactgatacc tttcttttcg taattacctt caatttctgt
     2401 ttcttcttga aatatagagc tagcctttaa ccccatatct tctgctcttt ccttttctgt
     2461 ttcaaatctt tctatatagc ttgcacttgt gacttctggt attgatatat tctctttgct
     2521 tggttcttta tctaatatct catttgtatc ttgctcgaag ttttcttctt tgctatgagg
     2581 agtagcaaaa tgttcttttc taatttttat aacaccttct tgttgctctt ctgattgatc
     2641 gtcagcttga tcttgtgata aagtttcatt aggagaaatt atatcaaaat atttcttatt
     2701 ttcatcctcg tctttttttg atattgtgtt tttagatctt ggcataaaac taaattatat
     2761 ttttcttatt tattattata aatattgtag cacaatttta aataaaattc aactacttta
     2821 atataa
//
LOCUS       DAOTJA010000070         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_29897_length_2756_cov_3.099593, whole genome shotgun sequence.
ACCESSION   DAOTJA010000070 DAOTJA010000000
VERSION     DAOTJA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29897_length_2756_cov_3.099593"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1582)
                     /locus_tag="P5241_03975"
     CDS             complement(80..1582)
                     /locus_tag="P5241_03975"
                     /inference="COORDINATES: protein motif:HMM:NF027116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32652.1"
                     /translation="MKEIKNYLAYFLIVSLILFNACEKEILKPDVAPNFRTIYGGVYD
                     SLTKKPIPGAYITIIPEGFPESKKDNIKTDNNGKFSADNMPVFSYGQKYYVTAVLPPV
                     YPNPNSITDGCDCDKLILKDIALLPDTSVLNHACDIFCIPPQIDFGCVAVNDSKEFSV
                     NLINSSSTGSNITINNLDIKSSEITVVSPSFPITLPPKGQKEMIVKWTPTKQQTNFTS
                     QIFANTDCNSNSKKILDLSGKSEFLTCSIRGNPLSGNVMNISASIGESNYGTFQITKI
                     SGCANLKTSVIKLGNDNHFKIDTSGLPNVVKITYDCTDTLEHYDSISIVTNGDCDKKF
                     ILYGNPYSSTLNGETLNPWSLCMRPKNKYTHRGYSFENKSVVDDEEILPGYTCTDDKF
                     NNPPVAMADFRFYGISGSMAIIQAANGLAYIDNVSKVDFNDPKKWYNSHIGKNYSNKS
                     LTVKNGDVIAIRTRQGKYALIWIRNIGINVQTGPSNTMDYLEWSYYYPAP"
     gene            complement(1598..2479)
                     /locus_tag="P5241_03980"
     CDS             complement(1598..2479)
                     /locus_tag="P5241_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32653.1"
                     /translation="MHLFEKALLSFSLFLLIAIQSHSCKAQDVIVKEDSIKIIRLKDG
                     KTYIGKVSKVPVEVSRNLKFNKIQIMPLKDGEYQEIITTDSTEVEFVTSYEQTIFPPS
                     RRSVAISSEIIPINKPGECDCECEKYYPILYWELKGLVMYSKSFSYGGETALGFRFTN
                     AFHFGFGGGAERIMKDWRFPLFLHLKYSFNSGDNYKVKPFIAGFAGYVYDKISNDYNV
                     KPALKNIFHPSPKMAGLSLGLDIPICCWLFDISMDLGYRYYTLPNEKELQNCDGNYFK
                     LGYDEVHAVIFRLGITF"
BASE COUNT          987 a          441 c          332 g          996 t
ORIGIN      
        1 aataaaatta tagtaattat aactaatata taaaaaattt ttgttttcat attattctcc
       61 aatttttttt cataatatat tatggagctg gatagtaata actccactca agataatcca
      121 tagtattaga tggacccgtt tgaacattta ttcctatatt tcttatccat ataagtgcat
      181 atttaccttg tcgagttcta attgcaatca catcaccatt ttttacagtc agagatttat
      241 tagaataatt tttcccaata tgactattat accatttttt aggatcatta aaatcaactt
      301 tagagacatt atcaatataa gctaaaccat ttgcagcttg aataatagcc atagaaccag
      361 agataccata gaatctgaaa tctgccatag caacaggtgg attattaaat ttatcatcag
      421 tgcaagtata accgggtaaa atttcttcat catccacaac agatttattc tcaaaactat
      481 aacctctatg tgtatatttg ttttttggcc tcatacataa tgaccaagga tttaaagttt
      541 ctccattgag tgtactagaa taaggattac catataaaat gaacttttta tcacaatcac
      601 catttgtaac tattgagatt gaatcataat gctctaatgt atctgtacaa tcataagtaa
      661 tttttactac atttggcaat ccacttgtat caattttaaa atggttatca ttgcctaatt
      721 ttatgacaga ggtttttaaa ttcgcacaac cagaaatttt tgttatttga aatgtaccat
      781 aattactttc acctatagaa gcactgatat tcataacgtt accacttaat ggattacctc
      841 ttatactaca ggttaggaat tctgatttac cagataaatc aagtattttt ttactattag
      901 aattacaatc ggtattagca aaaatttggg atgtaaaatt tgtttgttgt ttagttggag
      961 tccatttaac tatcatttct ttctgtcctt ttggtggaag tgttataggg aaagaaggac
     1021 taacaaccgt aatctctgat gactttatat ccaaattatt gattgtaata ttacttccag
     1081 tagaagaaga attaattaaa tttacagaaa attctttact atcatttacc gcaacacaac
     1141 caaaatcaat ttgtggtggt atacaaaaaa tatcacaagc atgatttaat acagatgtat
     1201 caggtaaaag tgcaatatct tttagaatta gtttatcaca atcacaccca tctgtaatag
     1261 aattaggatt tgggtaaact ggtggtagaa cagcagttac gtaatatttt tgtccatagc
     1321 taaagactgg catattatct gctgaaaatt taccattatt atcagtttta atattatctt
     1381 ttttgctttc aggaaaacct tcaggaataa tagtgatata ggctcccgga atcggttttt
     1441 tagttaatga atcgtaaact cctccataaa tagtcctgaa atttggagca acatctggtt
     1501 ttaaaatttc cttttcacaa gcattaaata atattaatga tactataaga aaatacgcta
     1561 aataattttt tatttctttc atatcatgtc tccaaaatta aaatgttatt cctaatctaa
     1621 aaataactgc atgaacttca tcataaccta acttgaaata attaccatca caattttgca
     1681 gttctttttc atttggcagt gtataatatc tataacctaa atccatagaa atatcaaata
     1741 accaacaaca aattggtata tctaatccca aacttaaacc tgccatttta ggagatggat
     1801 gaaaaatatt ttttaaagca ggtttaacgt tataatcatt agatatttta tcgtaaacat
     1861 aaccagcaaa acctgcaata aaaggtttta ctttgtaatt atcaccagaa ttgaaagaat
     1921 atttcaaatg taaaaacaac gggaacctcc aatctttcat tattctttca gcacctcctc
     1981 caaatccgaa atgaaaagcg tttgtaaacc taaaccctaa agcagtttca cccccatatg
     2041 aaaagctttt tgaatacatc actaaaccct tgagttccca atacaaaatt gggtaatact
     2101 tttcacattc acaatcacat tcacccggtt tattaattgg aattatttca gatgatattg
     2161 cgacggatct tcttgaaggt ggaaatattg tttgttcata ggacgttaca aattcaacct
     2221 cagttgaatc tgtagtaata atttcttgat actcaccatc ttttagtggc ataatttgaa
     2281 ttttgttaaa cttcaaattt ctactaacct caactggtac ttttgaaacc ttaccaatat
     2341 aggtttttcc atctttaaga cgtataattt taattgaatc ctctttcact attacatctt
     2401 gagctttaca gctatgcgat tgaatagcta taagtaagaa tagtgaaaaa cttaaaagag
     2461 ctttttcaaa taaatgcatt tgtccctctt agttaattaa aacaatatta ctattataaa
     2521 ttgataaagc ttaccttttt aattaaaaaa taaaaaagaa caattattaa atataaatat
     2581 gtattacgta gctaaaatga tatgcctaac aaaaccctac attctcaaat accatactca
     2641 tcccatattt tttcaaaaat ttataagaaa aaaaattaaa aagtcaatat tttttttatt
     2701 tttttcatct ataaacattt aatatttttg aatatttaat ttttttgggt taataa
//
LOCUS       DAOTJA010000071         2749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_29995_length_2749_cov_3.583148, whole genome shotgun sequence.
ACCESSION   DAOTJA010000071 DAOTJA010000000
VERSION     DAOTJA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29995_length_2749_cov_3.583148"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1894
                     /locus_tag="P5241_03985"
     CDS             425..1894
                     /locus_tag="P5241_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRV32654.1"
                     /translation="MSESKTKTELLRQIGIVTAISCSVGSMIGSGIFKKPGIMATQLG
                     SPELLVLVWIVTGLMTLFGALSIAEIAGMIHEPGGQYIYFNKSYNDFIGFLYGWAVFA
                     VIQTGSIASMAYVFSDSLGYFIDFPRFNSSIESFTIHLPFIGAITPLKFIGLKLCTIG
                     LILFLTAVNYIGVKFGSIVQVTFTSLKIFAIVAIVVLAIFLGHGNIDHFYKSADLSSL
                     GNTSIFLGFIMAMSGAFWAYDGWINVTYLAGEVKNPQKNLPRALFLAVVIVMAVYILV
                     NLAYIYIIPVDEMAMKYKQAEITGGSYLVATDVASSFWEGMGGTIIAAAIMLSTFGAV
                     NGTIMMSARVHFAMSKEGLFFKGLKNVHPKFNTPANSLLVQGLWASIMVFSGTFDQLT
                     DMIIFVSWIFYGTAAFGVFMLRKRWPDRYRPYKVWGYPVVPLLFVLFSFLYVVFTLYS
                     DVLNFVEGKSPLINSIMGVILVGLGIPGYVFWKRRKNNS"
     gene            2011..2625
                     /locus_tag="P5241_03990"
     CDS             2011..2625
                     /locus_tag="P5241_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRV32655.1"
                     /translation="MSLLNHTVGIITKPKVEWEAIKNEPMTIAQVFTGYVAILALIPA
                     IASFIGLSLVGVSIPGFYNYTIKYSIVGGLINAAVNYIMAFVGVYVAAFIINALASSF
                     ESTPNMENAVKLVAFSYTPVWLVGIFSIYPLLGILGILGLYSIYLLYLGLEPMMNTPA
                     TKKMAYLIVSIVVLIVIFIVVGLVVNLVTPKPYYGSYYEAPYNF"
BASE COUNT          871 a          333 c          483 g         1062 t
ORIGIN      
        1 gtttttaaca aatatagagt ttagaataga aagcaatagt ggtattaaca aaagtaataa
       61 atttattttt attgataaaa ttattgaatc aaaaaaattc tttttttcgt tttcgatgaa
      121 tacaatttta taagttatga gagagctggt aaaaaaagag aataatccta ttattgcaga
      181 aaattcataa cccagatagt taaataaact ctgtgaaaga agaattattg taataaaaaa
      241 gtatagtatt acatatagtc taatattttt gttttttaaa aaactcaaca taattgaaca
      301 cgcttaaaat agggatatat tttttcttgc aatataaaga tattattatt attatcaagt
      361 gattttgttt tttccggttt taaattatta ttaaaaaata aagaataatt acttaaggcg
      421 aattatgagt gaatcgaaaa caaaaacaga attgttaaga caaataggga tagtaaccgc
      481 aataagttgt agtgttggtt caatgatagg ttcaggtata tttaaaaaac caggtattat
      541 ggctacacaa cttggttctc cagagttact tgttttagta tggatagtaa caggtttgat
      601 gactcttttt ggtgcattaa gtattgcaga aattgcaggt atgatacacg agcccggcgg
      661 gcaatatata tattttaata agagttacaa tgattttatt ggttttctat atggttgggc
      721 tgtttttgct gtaattcaaa ccggttccat agcttcaatg gcttatgtat tttctgattc
      781 tttaggttat tttatagatt tcccaaggtt taactcatca atagaatcat tcactattca
      841 ccttccattt atcggtgcaa taactccttt aaaatttatt ggattaaaac tttgcacaat
      901 tggattgata ttatttttaa ctgcggtaaa ttatatagga gtaaaatttg gtagtatagt
      961 acaggttact tttacaagtt taaaaatttt tgctatagta gctattgtcg ttcttgcaat
     1021 ttttcttggg catggcaata tagaccattt ttataaatct gcggatctta gttcattagg
     1081 taatacatct atttttttgg gcttcataat ggcaatgtca ggtgcttttt gggcatacga
     1141 tggttggatt aatgttacat atttagcagg tgaagtcaag aatccacaaa agaatttacc
     1201 aagagctctc ttcttagctg ttgttattgt aatggctgtg tatattttag taaatcttgc
     1261 ctatatttat atcattcctg tagatgagat ggcaatgaaa tataaacaag ctgaaataac
     1321 aggtggatct tatctggttg ctactgatgt tgcaagtagt ttttgggaag gtatgggggg
     1381 tactataatt gccgctgcta taatgctatc tacatttgga gcagtaaatg gtacgataat
     1441 gatgtctgcc agagtccatt ttgcaatgtc aaaagaaggt ttatttttta agggacttaa
     1501 aaatgttcat cctaaattca atactccagc taattcgcta ttagtgcagg gcctgtgggc
     1561 ttcaattatg gtttttagtg gaacattcga tcaattaaca gatatgataa tatttgttag
     1621 ctggattttt tatggaacag ctgcatttgg tgtatttatg ctaagaaaaa ggtggcctga
     1681 tagatataga ccttataaag tgtggggata tccagtagtg cctttattat ttgttttatt
     1741 ctcattctta tacgttgtat ttactttgta cagcgatgtt ttaaattttg ttgaagggaa
     1801 gtctccatta ataaattcaa taatgggtgt tatccttgtt ggtttaggaa tacctggata
     1861 tgtcttttgg aagagaagga aaaataactc ttaaatttat taatattttt taattttttg
     1921 ttgcttttta ttagtttttt atataattta actttagaga atatttttca tctcaattaa
     1981 tttttttaat attttaaata gggaggacct atgagtcttt taaaccatac tgtaggaata
     2041 attactaaac ctaaagttga gtgggaggca atcaaaaatg aaccgatgac tatagcacaa
     2101 gtgtttacag gttatgtagc aattttagct ttgattcccg caattgcaag ttttattggt
     2161 ttatcattag ttggtgtatc aataccagga ttttataatt atacaattaa gtactcaatc
     2221 gttggtggtc ttattaatgc tgcagtcaat tatattatgg cttttgtagg tgtttatgtt
     2281 gcagctttta taattaatgc tcttgcaagt agttttgaat ctacccccaa catggaaaat
     2341 gctgtaaaat tagttgcatt ttcctataca ccagtttggc tagtaggtat tttctcgatt
     2401 tatcctcttt taggaatact tggtattctt ggtttataca gtatatatct tctttattta
     2461 ggccttgaac ctatgatgaa tactccagcc acaaaaaaga tggcttatct tatagtatct
     2521 attgttgtat taattgttat atttatagtt gttggtttgg ttgtgaactt ggttactcct
     2581 aaaccatact atggtagtta ttatgaagct ccttataatt tttagttaaa atattttgtg
     2641 ataaaaaacc ccttttataa aggggttttt ttattgtaca ttaaaataat ttaccagaaa
     2701 taaactcaat tgaggaatat aagttactaa aaggagagtt attatcatc
//
LOCUS       DAOTJA010000072         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_30233_length_2733_cov_5.703137, whole genome shotgun sequence.
ACCESSION   DAOTJA010000072 DAOTJA010000000
VERSION     DAOTJA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30233_length_2733_cov_5.703137"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..588)
                     /locus_tag="P5241_03995"
     CDS             complement(28..588)
                     /locus_tag="P5241_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HRV32656.1"
                     /translation="MPDDSYIIELKDNVRQKLNNYAEVLAAIRSNKISASLVENIIVD
                     CYGTQSSLKNLANIIVQPPNILIIEPWDSSILNELAHAIEVSPLHIMPQKDQKFLRLV
                     FPSLTTERREQLIKVINTEKEKIRIEIKQIREDIIKKVENDFQNKLISEDQKFYLKKE
                     IQKVIDDANTKIDELTIKKETDLKNE"
     gene            complement(807..2429)
                     /gene="groL"
                     /locus_tag="P5241_04000"
                     /pseudo
     CDS             complement(807..2429)
                     /gene="groL"
                     /locus_tag="P5241_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049565.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
     gene            complement(2497..>2733)
                     /locus_tag="P5241_04005"
     CDS             complement(2497..>2733)
                     /locus_tag="P5241_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRV32657.1"
                     /translation="YVFVEPLKEDNKTASGIIIPDDAEQKPILGKVIAKSAKVDSIKV
                     GDKVLFTKYSPTEVKIESQEYLVIKDEDILAVLS"
BASE COUNT          872 a          486 c          346 g         1029 t
ORIGIN      
        1 actaatggat taaaaaattt tttatgatta ttcgtttttt agatctgttt cttttttaat
       61 ggttagctcg tctatttttg tattagcatc atctattact ttttgaattt ctttttttaa
      121 ataaaatttt tggtcttctg atattaattt attttgaaaa tcgttctcaa ctttctttat
      181 aatatcttca cggatctgtt ttatttctat tcttattttc tctttttcag tattgattac
      241 ttttatcagt tgttcacggc gctcagtggt tagtgaggga aatactaatc gcaaaaattt
      301 ttgatctttt tgtggcataa tatgcaaagg cgaaacttct atagcatgag ctaattcatt
      361 caaaatacta gagtcccaag gctcaataat caatatattg ggaggctgga caattatatt
      421 agctaaattc tttaaagaag attgagtgcc atagcagtca acaataatat tttccacaag
      481 agaagcactt attttgttag aacgaatagc agccaaaact tcggcataat tatttaattt
      541 ctgacgaaca ttgtctttta attcaataat ataagaatca tctggcataa taaattttat
      601 ttagtatttg tttattattt atttctattg tatcatattt ttataaagaa tacaatagct
      661 taaaaatcat atcacttcaa tataataatg ccctttatac caaaaaagaa aaaatatata
      721 tctatatata gatataaaaa acaaattgta taaaaaatac tgccaaatat tgtataatat
      781 ttggcagtat ctatataacg ggacttttag tattccattg aaccaggcat ttctggataa
      841 gcattttctt ttttctcagg catatcaaca acaacagctt tggtagttag aaacatagaa
      901 gcaatcgatt cggcattttg taacgcactt ctcgtaactt tggttggatc aataatgcct
      961 ttatctaaca aattggtata ttcaaaattt aaagcatcaa acccttctca cacatctttg
     1021 ttttgaattt tttcaataac aaccgaacca tctactccac tattggctgc aatctgtcga
     1081 ataggcgctt ctaaagattt tttaataata tctataccga ttttttcatc ttcattatct
     1141 acttttatat ctgatatact atcaatacaa cgcaacaaag ctaccccacc acctggtaca
     1201 acaccttcct caatagcaga tttagtggct ctaactgcat catcaattct atcttttatt
     1261 tctttttgtt caagctcaga aggagcacca actttgataa ctgctacacc tcctgatatt
     1321 ttagctaaac gctcttgtaa tttttcttta tcaaattcag atgtcgaggt ttcgtattgc
     1381 tttcttattt gttgtttgcg tttttcgatt tctttggcgt ctcctttacc acctacaata
     1441 gttgtataat ctttggtaat aataactcta tgagctctac ctaacatttc caatgttgta
     1501 ttttctaatt tatgacctaa atcagaagat ataaactgag ctccacaaac agtggcaata
     1561 tcttgcaaca tttctttttg gttatcacca aatcctggag ctttgacagc tacacctgta
     1621 aaaatacctt ttaatttgtt gagaacaaaa gtagccaaag cttctccact aatatcatca
     1681 gcaataatta aaacttcttt tgaaccagcc tgtaataatt tatcaagtaa gggcaaaatt
     1741 tcttgaatag aagaaatttt tctatcagta accaaaatat aagcatcttc taaaacagct
     1801 tgcatttttt cagtatcagt aaccatatag ggagatatat atcccctatc aaattgtagt
     1861 ccttccacta actcttttga taaaccaaag gtttgagaat cttcaacagt tactacacca
     1921 tccttgccaa cagtatcaat tacatcagca atcaaattgc caatattagc atctctggca
     1981 gaaattgtag ctacttgggc aatttcgtct ttgttagaaa tagtcttgga aatagttttt
     2041 aaatgctcga cgactttggc actagctttg tccataccac gtttaatagc taagggatct
     2101 gaccctgcaa caatattttt caatccaata ttaatcatac tcgatgccaa aacaatagcc
     2161 gtagtagtac catcaccagc aacatcactc gttttttcag cagcttcttt aattatagaa
     2221 gcaccaatat tttctgtttt atcttttaat tctatctcct tggcaattgt tactccatca
     2281 ctcgtaagtt gaggagaacc ataacctttg tctaaaataa cattagatcc tttggggccc
     2341 aaagtaattt tgacagcatc ggttaattta tcaatgccgt tttttaaatt atttaaagca
     2401 ttttgatcaa aaataatttg tttagccata ttattttatt tattttttat ttatatatat
     2461 gtattaaata tattgattat ataatacact gatatattat gatagcacag ccaaaatatc
     2521 ttcatcttta attactagat attcttgcga ctctattttt acttctgtag gagaatattt
     2581 agtaaataaa actttatcgc cgaccttaat gctatctact ttagcacttt tggcaattac
     2641 tttacctaaa ataggttttt gttcagcatc atctggaata ataatgccac tagcagtttt
     2701 gttatcttct tttaatggct caacaaaaac ata
//
LOCUS       DAOTJA010000073         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_31018_length_2678_cov_5.833397, whole genome shotgun sequence.
ACCESSION   DAOTJA010000073 DAOTJA010000000
VERSION     DAOTJA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31018_length_2678_cov_5.833397"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1186)
                     /locus_tag="P5241_04010"
     CDS             complement(191..1186)
                     /locus_tag="P5241_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32658.1"
                     /translation="MLISIISVELTIIVFIKLQYYYILNIEPSSAIIIITINLALFSA
                     LFSILLKMIASQKKLKKEKEKKAIIISKIITEFRNILNILTEKFGVWENDNATKKLFD
                     SGIYKNVDYYEYKLMSKSDYTPKNNELINSLFKDSNHQNSQSDLYLSMISLVKNRNNN
                     KIIIDYLFDPYFIENDIYNIEFIEKCNTIYHIDSIWFWFNENCNFINYSNLGSSDISY
                     IEQSIKTIDPKYKFNELNDKTMAHICLKTSTYFNELYFILKFINRGIPLNNLFLIVCL
                     IFCLLFGILSPILFTIIHFNVFHTILITNILLSLNFGFIIFLIPYIFYLLRNDIS"
     gene            complement(1260..2579)
                     /locus_tag="P5241_04015"
     CDS             complement(1260..2579)
                     /locus_tag="P5241_04015"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRV32659.1"
                     /translation="MKTIKLLVTFLMLIIIHLNGLTQDYSTINIYYKNKLGSLNETIT
                     FNNTPIATMSLGSRLQCTMYSEGTLLIKSMNQYNGQDMIRLEIKKGNTYYIEMTAKGF
                     GSSWTGKLEVVDKVKGEMEFNKNKNWPLNYFEEDLYALIIRPKNPTYQNQVTNPQIIY
                     VETNQNAKYEYLPVSDIDKNIPETNSINEMSFALIIGNEDYATFQTDLSKEMNVEFAR
                     NDASAFKYYAIKTLGIPEKNITFLLDATYGQISQSLSKMNLIAKSTNGKATIYFYYAG
                     HGLPDQNTKEPYLMPVDVSGSNLSAAIKLKDVYSKLTEYPSERIIVFLDACFTGGGRD
                     QGLLSARAVKVRPREDLLTGNITVIASSSEDQTSLPYKDKKHGLFTYYLLKKIQETKG
                     DITLKELSEFLNEKVNLESILINNKEQTPKTNISLEVETTWQNWKLK"
BASE COUNT          903 a          355 c          346 g         1074 t
ORIGIN      
        1 gcaaatatat gaactttctg tttagcacgg tcggttgtaa tatagccgct aacggctggg
       61 ggctggtaaa gtgcaggagt ttgaaacgtt tcattgtccc acgctacgaa gctacaaaaa
      121 agcggaaatg tttcctaatc cgctggatac ctgcatttta ttagcccctt gttacccaac
      181 gtaattaact tcatgatata tcatttctta acaggtaaaa aatatacgga attaaaaaaa
      241 taataaaacc aaaatttaaa cttaataata tattagtaat taaaattgta tgaaatacat
      301 taaagtgaat tattgtaaat aaaatagggc ttaaaattcc aaataataag cagaaaatta
      361 aacagacaat caaaaataaa ttatttaaag gaatacctct gttaataaat ttaagaataa
      421 aatataattc attgaaatat gttgatgttt ttaaacaaat atgagccatt gttttatcat
      481 ttagttcatt aaacttatat ttaggatcta ttgtttttat tgattgttcg atataactta
      541 tgtcagatga acctaaatta gagtaattaa taaaattgca attttcatta aaccaaaacc
      601 aaatactatc tatatgatat attgtattgc acttttcaat aaattcaata ttgtaaatat
      661 cattttcaat gaaatatgga tcaaacaaat agtcaataat aattttgtta ttatttctgt
      721 tttttacaag gctaatcatg cttaagtata aatcagattg actattttga tggtttgaat
      781 ctttgaataa actatttatt aattcattgt tttttggagt ataatcactt ttcgacatta
      841 atttatattc ataataatca acatttttat atatacctga atcaaatagt ttttttgtag
      901 cattatcatt ctcccataca ccaaattttt ctgttaaaat attcaatata ttcctaaatt
      961 ctgtaattat ttttgaaatt attattgctt tcttttcttt ttcttttttt aatttttttt
     1021 gcgaagcaat catttttaaa agaatactaa ataaggctga aaataaagca agatttattg
     1081 tgataattat tattgctgat gaaggctcaa tatttaaaat ataataatat tgtagtttga
     1141 taaaaacaat gattgtgagc tcaactgaaa ttattgatat taacacaaac gataagtaag
     1201 tagaatacaa ttttgttata cgaaataata ttttgttgta aatcatttaa ttattagtat
     1261 tattttagtt tccaattttg ccatgttgtt tcaacttcta aacttatatt cgtttttgga
     1321 gtttgttctt tattattaat aagtatagat tcaagattaa ctttttcatt aaggaattct
     1381 gaaagttctt taagagttat atctcctttt gtttcttgaa ttttttttaa taagtagtat
     1441 gtaaaaagac cgtgtttttt atctttatag ggtaaagatg tttggtcttc actgctagaa
     1501 gcaattacgg taatatttcc tgttaataaa tcttctcttg gtctaacctt tactgctctt
     1561 gcagataaca aaccttgatc tctccccccg ccagtaaaac aagcatcgag aaatacaatt
     1621 attcgttcac ttggatattc agttaacttt gaataaacat ctttaagttt tattgcagct
     1681 gataagtttg aaccacttac atcaacaggc attaaataag gttctttcgt attttggtct
     1741 ggtaaaccat gcccggcata gtaaaagtaa atagttgctt tgccatttgt tgattttgca
     1801 attaaattca ttttgctcaa tgattggctg atttgaccat aagttgcatc tagtaagaaa
     1861 gttatattct tttctggtat tccaagtgtt ttaatcgcat aatatttgaa tgcacttgca
     1921 tcatttcgtg caaattctac attcatctct tttgataaat ctgtttgaaa tgtggcataa
     1981 tcttcattcc caattattaa agcaaaagac atctcattta tactatttgt ttcaggtata
     2041 tttttatcaa tatcagaaac aggtaaatat tcatatttgg cattttgatt tgtttcaaca
     2101 taaattattt gtggattagt tacttgattt tgataagttg gattttttgg tctaattatc
     2161 aaagcataca aatcttcttc gaaataattt aatggccaat ttttattttt attaaattcc
     2221 atttcacctt tcactttatc aacaacttcg agtttccctg tccagctact accaaatcct
     2281 ttagcagtca tttcaatgta ataagtattt ccttttttaa tttctaacct tatcatatct
     2341 tgcccattgt attggttcat actttttatc aacaatgtac cttcagaata catagtacat
     2401 tgtaatctag aacctaaaga cattgttgca ataggtgtat tattaaatgt gatagtttcg
     2461 tttaatgaac ctaatttatt cttatagtag atattaattg tggaataatc ttgagttaaa
     2521 ccatttaaat gtataattat caacattaaa aatgtaacta aaagtttaat tgttttcatt
     2581 attattaagt ttaagtttgt taatatagtt tatgttattc gcaatcgttt tgtcattatg
     2641 ttgggtaacg gtttcggtat atgaaaagtt gggctata
//
LOCUS       DAOTJA010000074         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_31489_length_2646_cov_2.800463, whole genome shotgun sequence.
ACCESSION   DAOTJA010000074 DAOTJA010000000
VERSION     DAOTJA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31489_length_2646_cov_2.800463"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5241_04020"
     CDS             complement(<1..194)
                     /locus_tag="P5241_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRV32660.1"
                     /translation="MKRKKIIGIFCNGEKINKKIFSLFSNEINFIICADGGSDKIHHL
                     NIVPDVIIGDMDSISNKALM"
     gene            complement(452..973)
                     /locus_tag="P5241_04025"
     CDS             complement(452..973)
                     /locus_tag="P5241_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRV32661.1"
                     /translation="MKKLIGKKIVIVIAPSMFRDEEFQVPYEYLKSEGADVFIASTTL
                     SEAVGKLGLRVKPDITINKISYDVFDAIVFVGGPGVKAYWDDKSIHKLAKSFLENNKI
                     VAAICSAPVILARAGLIKNKKVTSFPGDEEEMKKSGCIYTSAVVEKDGKIITGNGPAA
                     AKSFAETIAKSLS"
     gene            1106..1291
                     /locus_tag="P5241_04030"
     CDS             1106..1291
                     /locus_tag="P5241_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRV32662.1"
                     /translation="MQKYFNLVEKVGLSEKSIEEAIENAVLSENSVSWFEVVEIRGRV
                     NQDKKIEYQVKVRIGTK"
     gene            1336..1803
                     /locus_tag="P5241_04035"
     CDS             1336..1803
                     /locus_tag="P5241_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRV32663.1"
                     /translation="MSLKVGDKAPDFKSKTHLMEDFTLSSLFGKENIVLLFFPLVNTG
                     TCFNEMCTLRDSMHDYEKLNAKVIAISVDSPFAQKLWAEKEKFNFTFVTDFNKEISKK
                     YGCLYDSLVGLNGVSKRSAFVIDKEGTLRYLWVSEDAGVLPDFNAIKEVLSKL"
     gene            1838..2194
                     /locus_tag="P5241_04040"
     CDS             1838..2194
                     /locus_tag="P5241_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV32664.1"
                     /translation="MACLFLKYLILFMIKELDVFELKNKIDNNENFYLLDVRETWEYK
                     LCKIPNSMLIPFGILPLKINELDPEKEYILYCHHGTRSFFACQILLKNGFKKIYNLKG
                     GIHEYSRKVDNSIKQY"
     gene            2199..>2646
                     /locus_tag="P5241_04045"
     CDS             2199..>2646
                     /locus_tag="P5241_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRV32665.1"
                     /translation="MNKIKYISLLFSLIILISSLANSQKKDIIKDMVLIPAGYFNLGS
                     NNGYDDEYPIVKVWLDSFYIDSHEITNAEYKIFCDSTKTPYPPNPPWDTAYFTKKPNY
                     PVINVTWNEAQKYANWVGKRLPTEAEWEKACKAGADTKYFCGDTITN"
BASE COUNT         1007 a          333 c          389 g          917 t
ORIGIN      
        1 ttcattaaag ctttatttga aatagaatcc atatcaccta ttataacatc aggtacaata
       61 tttaaatgat gaattttgtc agagccgcca tcagcacata taataaaatt gatctcatta
      121 gaaaataaag aaaaaatctt tttattgatt ttttctccat tacaaaatat acctataatt
      181 ttttttcttt tcatattttt tattgataat aagcaaaaaa aaaattaaat gaaatgaaat
      241 aaagtaaaaa aactttgaca aaactaattt tttaaatgga tatgttaaat agagaaaagt
      301 attagtaatg tttataaatt gtatataaaa gagtaatact attattaata atacaaatat
      361 taataataaa ttaattatac tagaaaattt atcaataaaa tgattgccgg gtacaggtaa
      421 aaattaaata tttataaact attatatgtc tttaactcaa agattttgca attgtttctg
      481 caaaagattt tgctgctgct ggtccattac ctgtaataat ttttccgtct ttttctacca
      541 ctgctgatgt ataaatgcat ccactttttt tcatctcttc ttcatcacca gggaaagaag
      601 taaccttttt attttttatt aagcctgctc tggcaagtat tacaggtgca gaacaaattg
      661 cagcaactat tttgttattc tcaaggaaac tttttgctaa tttgtgaata gatttatcat
      721 cccaataagc ttttacacca ggaccaccta caaaaacaat tgcgtcaaaa acatcatacg
      781 aaattttatt gatagtaata tctggcttaa ctcgtaagcc tagtttccca accgcttcgc
      841 tcaatgttgt cgaagcaata aaaacatctg ctccttcaga ttttaaatat tcgtaaggta
      901 cctgaaactc ttcatctcgg aacatactag gagcaatgac gattacaatt tttttcccaa
      961 ttaatttttt cattgtttta aaaaaaaggt ttattatttt aataaagtta agggaactaa
     1021 atcaactata attagcgtta catatttaaa gatatagtaa tgattactaa atttcaagtc
     1081 aaacattatt taataaggag gctatatgca aaagtatttt aatttagttg aaaaagtagg
     1141 tttatctgaa aaaagtattg aagaagcaat tgaaaacgct gttttatctg aaaattctgt
     1201 tagctggttt gaagtagttg aaattagagg tagagttaat caagataaaa aaattgaata
     1261 tcaggtaaaa gtaagaattg gaacaaaata ataaaagtaa taaatatcaa taaacattta
     1321 aattaagagg aatatatgtc tctaaaagtt ggtgacaaag cacccgattt taaatcaaaa
     1381 acacatttga tggaagattt taccttatct agtttgtttg gtaaggaaaa catagttttg
     1441 ttattttttc cattagtaaa tactggaact tgttttaatg aaatgtgtac attaagagat
     1501 tccatgcacg attatgaaaa gttaaatgct aaagttatag ctataagtgt tgatagccca
     1561 tttgcacaaa aattatgggc agaaaaagaa aaattcaatt tcacttttgt aacagatttt
     1621 aacaaagaaa tttctaaaaa atatggttgc ctttatgata gtctggtagg tcttaatggt
     1681 gtttcaaaga gatcagcttt tgtaatcgat aaagaaggta cattgcgtta tctatgggtt
     1741 tcagaagatg ctggtgtatt gccagatttt aatgctataa aagaagtatt atctaagctt
     1801 taacagttaa agccattacc ctattgttga tggagtaatg gcttgtttat ttctaaagta
     1861 tttaatatta tttatgataa aagaattaga tgtatttgag ttaaaaaata aaattgataa
     1921 taatgaaaac ttttatttat tagatgttcg tgaaacctgg gaatataaat tgtgcaaaat
     1981 tccaaattca atgttaattc catttggaat attaccatta aaaataaatg aactagatcc
     2041 agaaaaagaa tatatattat attgtcatca tggaaccaga agcttttttg cttgtcagat
     2101 tcttttgaaa aatggtttta aaaaaatata taatcttaaa ggtggtattc acgaatattc
     2161 aagaaaggta gataatagta taaaacaata ttaaatacat gaataaaata aaatacattt
     2221 ctctattatt ttcccttatt atacttatta gctcgttggc taattcccaa aaaaaagata
     2281 ttataaaaga catggtactt attcctgctg ggtattttaa tttaggctct aataatggtt
     2341 atgatgatga atatccaatt gtgaaagttt ggcttgattc cttttatatt gatagtcatg
     2401 aaatcactaa tgctgaatac aaaatatttt gcgatagtac gaaaacaccc tatccaccga
     2461 atccaccatg ggatactgct tattttacaa aaaagccaaa ttacccagtg ataaatgtaa
     2521 cctggaatga agctcaaaaa tatgctaatt gggttggtaa aaggttacct acagaagcgg
     2581 aatgggaaaa agcatgtaag gctggggctg atacaaaata tttttgtgga gatacaatca
     2641 caaatg
//
LOCUS       DAOTJA010000075         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_31901_length_2621_cov_2.922058, whole genome shotgun sequence.
ACCESSION   DAOTJA010000075 DAOTJA010000000
VERSION     DAOTJA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31901_length_2621_cov_2.922058"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1025
                     /locus_tag="P5241_04050"
     CDS             264..1025
                     /locus_tag="P5241_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32666.1"
                     /translation="MKNLHYLKIFVIILFCFLLTKTSELYSQEYDYNDYEIKNPRYGF
                     GIGFNPFYILHDFSDVNQKLSISGYNKLSKDNIVSYGGSFYIYTGFVQNLRVGGFWSQ
                     ADKNSSKKDNNLIKDFDYNFSFWAVNVDYALPLAKRLSLVFGSFFGKGTQNYDISEKY
                     NNDIDWEELWNSSSRVIDNKTNLNSSFFIFSPYVLLEYALLDFLMIRIGGGYIGSAKT
                     DLNYENFYPVKNVPSSLRINDFYFQFGILTGLFME"
     gene            1042..2361
                     /locus_tag="P5241_04055"
     CDS             1042..2361
                     /locus_tag="P5241_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRV32667.1"
                     /translation="MSLKEKIISKKAVIGVVGLGYVGLPLAVEYANKGFKTYGIDVDK
                     KKIDSLKKRINYILDIPKEDFDKAISSGNFSATENYSIVKDLDVIFICVPTPFTKNKE
                     PDISYIVDASKNIAKYLKKDKIVILKSTTFPNTTEGYVQPILESTGLKVGKDFYLAFS
                     PERIDPGNKVFTTANTPVVVGGVTKKCTELSALITSQAIAKVHTVSSPKVAEMEKLLE
                     NIFRSVNIALVNELARLCDRMGGINMWEVVEAASTKPFGFMPFYPGPGIGGHCILIDP
                     YYLSWQAREFDFQTKFIELAAETNENMPFYVRNLILREISKMPKTHKDAKILFLGAAF
                     KKNVDDTRHSPAIRVMELMIAEGAKNVYYTDPYVKEITVNNKIYKSKPLNEKLLKESH
                     IVVITTDHDCFDYDLVVKNSKCIIDTRNALKNIKNKKAKIKLLGDGK"
     gene            2378..>2621
                     /locus_tag="P5241_04060"
     CDS             2378..>2621
                     /locus_tag="P5241_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRV32668.1"
                     /translation="MKKIISVVGARPNFMKVAPIYRELLNFKNNINHLIVHTGQHYDE
                     KMSKIFFDDLELPKPDIYLGVGSGTHSVQTAKIMIEF"
BASE COUNT          967 a          301 c          399 g          954 t
ORIGIN      
        1 tttataatta atttaccata tccataaaat tgcagatact ttttattatt gcatatggac
       61 acatgttaat tctttcctat atattattat catattaata atatttactt aatcatcact
      121 aatttttata aatgcaattt tatttgaatt taactttgaa attaaattag aaaattaaat
      181 ataactaaat tttaatggtt aaataatagt gttgatttta ttctttattt ataatatttt
      241 agttttatta ttaattattt tatatgaaaa atttacatta tttaaaaatt tttgtaataa
      301 tattgttttg ttttctttta acgaaaacaa gtgaattgta ttctcaagaa tacgattata
      361 acgattatga aattaagaat cctcgttatg gcttcggtat tgggttcaat cctttttata
      421 ttttacatga tttttcagat gttaatcaaa aactttctat ttctggttat aacaagcttt
      481 ccaaagataa catagtttct tatggtggtt ctttttatat ttacactgga tttgtacaaa
      541 atcttcgagt aggtggtttt tggtcacaag cagataaaaa ctcatcaaaa aaagataata
      601 atttaattaa agatttcgat tataatttta gtttttgggc agtaaatgta gattatgcat
      661 tacctttagc aaaaagacta agcttagtat ttggttcctt ttttggaaaa ggtactcaga
      721 attatgatat ttcagaaaaa tataacaatg atattgattg ggaagagttg tggaattcaa
      781 gttctagggt aattgataat aaaactaatt taaattcatc tttttttatt ttttctcctt
      841 atgtgctatt agaatatgca ctattggatt ttttaatgat aagaataggt ggtggttaca
      901 ttggaagcgc taaaacagat ttgaattatg aaaattttta ccccgtaaaa aatgtccctt
      961 cttcattacg tattaatgat ttttattttc aatttggaat actaacaggt ttatttatgg
     1021 aataaattat aggattatct tatgagtttg aaagaaaaaa ttatttcaaa aaaagcagtt
     1081 attggagttg ttggacttgg ctatgtagga ttgcctcttg cagttgagta tgccaacaaa
     1141 ggttttaaaa cttacggtat tgatgttgac aaaaaaaaga ttgattcttt aaaaaaaaga
     1201 ataaattata ttcttgatat accaaaagaa gattttgaca aagcaatatc aagtggtaat
     1261 ttttccgcta ccgaaaatta ttcgattgta aaagatttag atgtaatttt tatttgtgtc
     1321 ccaactccat ttacaaaaaa taaagaacca gatatttcat atatcgtaga tgcatctaaa
     1381 aacattgcaa aatatttaaa aaaagataag atagttatat taaaaagtac gactttccct
     1441 aatactactg aaggatacgt tcaaccaatt ttagaatcaa caggtttgaa ggtaggtaaa
     1501 gacttttatt tagctttttc tcctgaaaga attgatccag gtaataaggt tttcactact
     1561 gctaataccc ctgtagtcgt tggtggagtt actaaaaaat gtacagagct atcagcacta
     1621 attacttctc aagcaattgc taaggttcat actgttagca gtcctaaagt agctgagatg
     1681 gaaaagttat tagaaaatat atttcgaagt gtgaatatcg ctcttgtaaa tgaattagca
     1741 agattatgcg atagaatggg tggaattaat atgtgggaag ttgtagaagc tgcttctacc
     1801 aaaccttttg gttttatgcc tttttatcct ggtcccggaa ttggaggtca ttgcatttta
     1861 attgatcctt attacttaag ttggcaagca agagagtttg attttcagac aaaatttatt
     1921 gaattagctg ctgaaacaaa cgaaaatatg cctttttatg taagaaatct tattcttcgt
     1981 gaaataagta aaatgcctaa aactcataaa gatgctaaaa ttttattttt aggtgcagct
     2041 ttcaagaaaa acgttgatga tactagacat tctcctgcaa ttagggttat ggaattaatg
     2101 atagctgaag gagcaaaaaa cgtatattat actgatcctt atgtaaaaga aataactgta
     2161 aacaataaaa tatataaatc aaaaccttta aatgaaaaat tacttaagga atcacatatt
     2221 gttgtaatta cgactgatca tgattgtttt gattacgatc tcgttgtaaa aaattcaaaa
     2281 tgcataatag atacacgaaa tgctttaaaa aatataaaaa ataagaaagc taagattaaa
     2341 ttattaggag acggtaaata atagtacgga gtaaaaattg aaaaaaataa ttagcgtagt
     2401 aggagcaaga ccaaatttta tgaaagttgc tccaatttat agagaattgc ttaattttaa
     2461 aaataatata aatcatttaa ttgttcatac aggccaacat tatgatgaaa aaatgtcaaa
     2521 aatatttttt gatgacttag agttaccaaa gccagatatt tacttaggtg ttggttcagg
     2581 aacacattca gtacaaactg caaaaattat gattgaattt g
//
LOCUS       DAOTJA010000076         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_34042_length_2491_cov_4.484401, whole genome shotgun sequence.
ACCESSION   DAOTJA010000076 DAOTJA010000000
VERSION     DAOTJA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34042_length_2491_cov_4.484401"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="P5241_04065"
     CDS             <1..201
                     /locus_tag="P5241_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRV32669.1"
                     /translation="KFYNYLFKRFINYAELFLSRIKDSPKERYEDLVKNYPDIIKRVP
                     QHYIASYLGITPVSLSRIRNKK"
     gene            556..1035
                     /locus_tag="P5241_04070"
     CDS             556..1035
                     /locus_tag="P5241_04070"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HRV32670.1"
                     /translation="MGKYINNIGLFLSGVFTMFSGMFIQLKYHIGNHNHIKTNQIVFN
                     LDYKTWSYFHKISIVIFSLLMIFHFYQHWKFYKIVFTKKIITKNKQVIVLTLVFVFVA
                     ITGFIPWFIDVLNGNDIIRKSIIEVHDKIAIILTIYLILHIKKRIKWFLTTLKSLKV"
     gene            1648..1854
                     /locus_tag="P5241_04075"
     CDS             1648..1854
                     /locus_tag="P5241_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016647569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRV32671.1"
                     /translation="MPNGKVKWFDPKKGYGFISQDKGEDVFVHFSEIKTDNEFKTLEQ
                     NQPVTFEIVESKRGLQAHNVVLTK"
     gene            1880..2335
                     /locus_tag="P5241_04080"
     CDS             1880..2335
                     /locus_tag="P5241_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32672.1"
                     /translation="MAFSFQLEDFKKIGDVLGVKPKFDSNVYRYDIQKENPVRHLTLE
                     IYPDIIIGKTKGNLISVYSVNSHLQLHFCNGFVASEILGEVTFFTDQCNKVSGLIIEK
                     EGGCSLFSNVDREVLSGDFTQLAPEVMMSSIALSLMETTLPDEENEEKE"
BASE COUNT          908 a          271 c          370 g          942 t
ORIGIN      
        1 aaattttata actacctttt caaacgattt atcaattatg cggaattgtt tttatctcgt
       61 atcaaagatt caccaaaaga gagatatgaa gatttggtaa aaaattaccc tgatattatt
      121 aaaagagttc cacaacatta tatagcttcc tatttgggta taactccagt ttcacttagt
      181 cgcattagaa ataaaaaata agtttcattt cttaacaatt gttaatgtag taataataat
      241 tttttgagta tatttactaa gaataattta aaaattaatt tattaattaa aaatggaaat
      301 tattttttta aaatcaaata ttataaaaac tgcatttgtt gttctagata ctaaaaaatg
      361 tgaagggtgc tggaattgtt ttaaagtttg taaaaataat gttattagtc gtataaactt
      421 tctatggcat aaacatataa ttttagttaa tgtagataga tgagttggtt gccttaaatg
      481 tgtaaaagtt tgtaaaaatg gagcaatcat tccttttata aatttgggga agtagataaa
      541 tggggaagta gataaatggg gaagtatata aataatatcg gattgtttct ctctggtgtt
      601 tttacaatgt tttcgggtat gtttattcaa ttgaaatatc acattggtaa tcataaccat
      661 attaaaacaa accaaattgt ttttaattta gattataaaa cttggtcgta ttttcataag
      721 atttcaattg ttatattttc tctgctaatg atttttcatt tttatcagca ttggaagttt
      781 tataaaattg tatttactaa gaaaatcatt acaaaaaata aacaagtaat agttctaaca
      841 ttagtttttg tatttgttgc aattacaggg tttattcctt ggtttattga tgttttaaat
      901 ggtaatgata ttattcggaa aagtattatt gaagtgcatg ataaaatagc aataattctt
      961 actatatatt taatcttaca cattaaaaaa agaattaagt ggtttttaac aactttgaaa
     1021 agtttaaaag tttgagaagg tttataagtt atatggatgt gaattaaaat ttcttaaaca
     1081 atttttatgt ttgaattata caattattat gataattaac aaaaagctta ttactattct
     1141 aattatgata caattattac aatttcaaat taaatataaa tctgttgaat ataaattata
     1201 atcttatcgc ttccacttaa ttcgctaatt tcccttttag gtataacccc ttttttattg
     1261 aaaaccaatt aaaaactcac aataattttt tattcaatta agatgtaaac tcacatttga
     1321 ggttatttgt tttaaggata attaaactaa tattaaaata atcgattaga tgtaataaat
     1381 ataggtgcaa aaatttaaag atttttattg cttttcagaa aaatattgtt tattttatat
     1441 cactggaaaa tgtataaaaa aatttggaca aattatcagt tttttaagac caatctaatc
     1501 agtgaaaaat cattatattg gattaaaatg aacattaggc gtctaagttt taactcttat
     1561 ttcagaatgt attttttcca ggtatcaatt aacaatattt tttaacattt tcaagcttca
     1621 attagtaaaa gcttaggagt attttttatg ccaaacggta aagttaaatg gtttgatcct
     1681 aaaaagggtt atggttttat tagtcaagac aaaggtgaag acgtctttgt tcatttttct
     1741 gagattaaaa ctgataatga attcaaaacc ctggagcaaa atcagcctgt tactttcgaa
     1801 atagtagaaa gtaaaagagg tcttcaggca cataatgttg ttctaacaaa gtagaacgta
     1861 actttaatag aggtagaaga tggcttttag ttttcaacta gaagatttta agaagatagg
     1921 tgatgttctt ggtgtaaaac caaaatttga ttcaaatgtt tatcgttatg atattcaaaa
     1981 agaaaatcca gttaggcacc ttacacttga aatctatcca gatattatta ttggaaaaac
     2041 taaaggtaat cttatttctg tctattctgt taattctcat ctccaattgc atttctgtaa
     2101 tggatttgtt gccagtgaaa ttctgggcga agttactttc tttactgacc aatgtaataa
     2161 agtatcagga ttaattatag aaaaggaagg aggttgttct ttattctcta atgtagatcg
     2221 tgaggttctt tctggtgatt ttactcaatt agcaccagag gtaatgatgt ctagtattgc
     2281 tttatcatta atggaaacca cattgccaga tgaagagaat gaagaaaagg agtgatggaa
     2341 taactgtaat aaatctttta cctcgattca aatttccaca tacaatagca agaaaattgt
     2401 tattgagagt tttatcgcat tttcaattta aagttgaaag tgtcaacatt gtgtttatgg
     2461 gtgatagaga tatcacccat attaatgtac a
//
LOCUS       DAOTJA010000077         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_36526_length_2359_cov_2.951823, whole genome shotgun sequence.
ACCESSION   DAOTJA010000077 DAOTJA010000000
VERSION     DAOTJA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36526_length_2359_cov_2.951823"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1097)
                     /gene="rfaE1"
                     /locus_tag="P5241_04085"
     CDS             complement(99..1097)
                     /gene="rfaE1"
                     /locus_tag="P5241_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRV32673.1"
                     /translation="MDIVIKNNILDVILRFENKRIAIIGDLMLDRYLWGSAIRISPEA
                     PVPVVDIDQESNRLGGSANVANNIITLGGIAYPIGLIGNDHNGNIILELMNSDKFITD
                     GIIKDPNLPTTVKTRVIANNQHVVRIDRENKHTISEKSYQLLFNFIEANIKSFDAIIF
                     EDYNKGVITKELIKNVINLAKKHSIPVTVDPKFDNFFEFVNVDVFKPNRKEAEEAIGQ
                     KIKSDEDLFKVMQSIQRKINCANLLLTRGEKGMALMEKNGEITLISTRARNIADVSGA
                     GDTVISTLTMALVSGATPKEAAVISNFAAGIVCEEVGVVPIRKDILINKIQEELTK"
     gene            1339..2151
                     /locus_tag="P5241_04090"
     CDS             1339..2151
                     /locus_tag="P5241_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRV32674.1"
                     /translation="MKSLKEKIYSLTEMTYEEGSKKKIYDFFDIFMIVLIVLNVIAVI
                     LETEHWLYQKYQYFFDIFDEISVYIFSIEYISRLWACTVNPLYSRPIIGRIKFALTPL
                     LIVDFIAIIPFYLPMFFPDMRFIRSIRLFRLFRAFKFVRYSETLQTFGNVIKLKKVEL
                     MTSLMMVIFLLIFSSSVMYYVEHEAQPNAFSSILATMWWGIATLTTVGYGDIYPITPI
                     GKLFGAFIAILGIGMVALPAGILASGFSSELSNKMDKKECEEVCPHCGKKIR"
BASE COUNT          785 a          335 c          332 g          907 t
ORIGIN      
        1 gcaaccgttt gtaaagacga tttttttgtt attatttttt attgcttttc ttatatttac
       61 tagttcatcg atagttaata ttatacccat taaagccttt attttgttaa ctcttcttgt
      121 attttgttaa ttaatatatc ctttcttatt ggtactacac caacctcttc gcatactata
      181 ccagcagcaa aatttgagat tacagcagct tcttttgggg ttgcaccgct tacaagagcc
      241 atagtaagag ttgaaattac tgtatcacct gcaccagaaa catcagcaat atttcttgca
      301 cgtgtactta tgagagtaat ttcaccattt ttttccatta aagccattcc tttttcacct
      361 ctggtaagta ataaattggc acagttaatt tttctttgaa tgctctgcat cactttaaaa
      421 agatcttcat ccgattttat tttttgacca attgcttcct ctgcttcttt tctgtttggt
      481 ttaaatacat caacatttac aaattcaaaa aaattatcaa attttggatc cacggtaaca
      541 ggaatgctat gtttttttgc aaggttaatc acatttttaa taagttcttt tgttattaca
      601 cctttattgt aatcttcgaa aatgatagca tcgaatgatt taatattggc ttcaataaaa
      661 ttgaatagca attgatatga tttttctgaa attgtatgtt tgttttctct atctatacga
      721 acaacatgtt gattgtttgc aataacgcga gtcttaacag ttgtaggtag gttcgggtct
      781 ttaattattc catcagtaat aaacttatca gagttcatta gctctaaaat aatattacca
      841 ttgtggtcat taccaattaa accaataggg taagcaatac ctcccaaagt tataatatta
      901 tttgcaacgt ttgcagaacc acccaaccta ttagattcct gatcaatatc tactacaggt
      961 acaggagctt ctggagaaat tcttatagcg cttccccata agtacctgtc taacattaaa
     1021 tcaccgatta tagcaattcg tttattttca aaccttaata taacatctaa aatattgttt
     1081 ttaatcacga tatccacaat atattattta ttaataatag caatataaac aaggtaataa
     1141 tgaatttcaa gtgaaaaact ttaaataaaa tttaattgaa tagaaactcc tttctcaaaa
     1201 cgagtgtaga ggtaaaactt cttatatttt ttaaaaatgt taattacttg aagataaatg
     1261 actgaaagaa tatataattt ttttgaaatt tatttttttt atgaatatat tatacaaaac
     1321 tatagaggtg atggtgttat gaaatcgtta aaagagaaga tttacagtct tacagaaatg
     1381 acttatgaag aaggttcaaa gaaaaaaatt tatgattttt ttgatatttt tatgatagtg
     1441 ttaatcgttc taaacgttat agctgttata cttgagactg aacattggct atatcaaaaa
     1501 tatcaatact tttttgatat atttgatgag atatctgttt atatattttc aatagaatat
     1561 atttcaaggt tgtgggcttg tacagttaac ccgttatact ctagacctat tattggtagg
     1621 ataaaatttg ctttaacacc tctccttatt gtagacttta ttgcaatcat acctttttat
     1681 ttgccaatgt tttttcctga tatgaggttt attagaagta taagattatt taggttgttt
     1741 agagccttta aatttgtaag atactcagaa actttacaga catttgggaa tgtaataaaa
     1801 cttaaaaaag ttgaactaat gacttcattg atgatggtaa tatttttgct aattttttca
     1861 agtagtgtga tgtactatgt tgaacacgaa gcacaaccga atgcattttc ttctattctt
     1921 gcaacaatgt ggtggggaat tgcaacatta actactgttg gctatggtga tatttatcca
     1981 ataactccaa taggaaaatt attcggtgct tttatagcca ttcttggaat aggtatggta
     2041 gctttacctg ctggtatttt agcttccggg ttttctagtg aactatcaaa taaaatggat
     2101 aaaaaagaat gcgaagaagt ttgtcctcat tgtggaaaaa aaataaggta aggtttaaat
     2161 ttacatatat aaattaaagt aatcgtaaat acaaatgtaa ttgcaaaaaa accaacacat
     2221 atactgcttt aattattttg acaattcatt tcaataaagt ttttttaact aaaaaatttt
     2281 tttgttctat tgaagaatat ctgtcttaaa ttattcaatt gctcttttaa atatttatga
     2341 ttaattacta ataatttgg
//
LOCUS       DAOTJA010000078         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_38081_length_2285_cov_3.164574, whole genome shotgun sequence.
ACCESSION   DAOTJA010000078 DAOTJA010000000
VERSION     DAOTJA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38081_length_2285_cov_3.164574"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="P5241_04095"
     CDS             <1..268
                     /locus_tag="P5241_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32675.1"
                     /translation="WNVGELSVAINGSNVTLTITLKPEYKMSELHIYINDKKPTTSAP
                     GQYGFTATFDPKTGSYTKTFTLSDTDNDGIWIIVHMVTWGVFQK"
     gene            1085..1396
                     /locus_tag="P5241_04100"
     CDS             1085..1396
                     /locus_tag="P5241_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRV32676.1"
                     /translation="MNYSNYYSENKFWKKIKTVGRSIPFSRDVIALFYCLCDPNTPKW
                     VKVIIIFALGYFICPLDAVPDLIPIAGYGDDAGVIASALASISAFVRKEHYKKADEFL
                     S"
     gene            1517..2263
                     /locus_tag="P5241_04105"
     CDS             1517..2263
                     /locus_tag="P5241_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRV32677.1"
                     /translation="MKNNLIVLLIVLSNILFAQSSDSIKARAIFDWITDNIEYDIEAY
                     QNNKVSLVQCDVENVLKTKKAVCEGYARLFQKMAQDSGLECKVITGKSKTFGFGDHAW
                     NAVKIDSVWRLVDACWGAGGYRGNRFIKSKNLFYFFTPPEKLILTHFPDFQEYQFLKN
                     PVTRQEFFGSENTGYSPVMYTLLLAEVSDYSYAEMIAMNVVTGYGRAVITYDPATKKY
                     LVYSGQFTSEKEAIEASKTVENCSVKKLVK"
BASE COUNT          869 a          246 c          371 g          799 t
ORIGIN      
        1 ttggaatgtt ggtgagcttt cagtagctat taatggatct aatgttactt taacaattac
       61 attaaaacca gaatacaaaa tgagtgaatt gcatatatat attaacgata aaaagcctac
      121 aacttccgca cctggtcaat atggttttac agctacgttt gatccaaaga cgggatctta
      181 tactaaaacc ttcactctaa gtgatacgga taatgatggc atttggataa tagttcatat
      241 ggttacttgg ggtgtatttc agaaataaaa atttacggtg ggggatttct atcctccacc
      301 ttttcatttc ataattatcc tttcattcaa atcattttta attatctaat acaacaaaaa
      361 gtaataagtt gttaaaaata gatttatcct ttattaaatc tagtagcaat tagttttata
      421 taaaaatttt ctaaataaaa ttgacctcgt ataatatatt cttacttaat gataagaaat
      481 attatttatt gttaaatttt ttagaatagt atcgtaccat aaaactataa tatggaagta
      541 taaaaagcag ttttgatatt tattcgaaat caaagggtaa taggtgcttg aataagtcaa
      601 agttatggtt agtttctttt tctataatat cttaaaaaat atatatgaaa tttcttatat
      661 ctaaaattta ttttgttact caaatatttt taaatattag ggggttataa aaaaaataaa
      721 aaataattac attttgcaac aaaaaacaat ctcaattgtg taaaagaaaa attaagaaaa
      781 atatttttaa aggataatag atgaaataaa aataaaataa attctataga aaaaatttag
      841 gtcacaaaaa aaacaggtat aagaaatggc ataaaatttg agttagaata attaaagaat
      901 gaaacaaatt taaagagtga gtatatgata tgtaataata gattgctaaa cttttctatg
      961 aaaacagaaa atttaatatt tgtttttaga gtgactttaa agataactgt aaaattattt
     1021 ttaataggtt atatgtgttt ttaaaataat aatatgtttt tcattattaa ataaggagaa
     1081 taatatgaat tattcaaatt attattcaga aaacaaattt tggaaaaaga tcaaaacagt
     1141 aggtagatca ataccatttt cgagagatgt gatagcattg ttttactgtc tgtgcgatcc
     1201 caatacacca aaatgggtga aagtgataat tatttttgca ttaggatact ttatttgtcc
     1261 actagatgca gtacctgatt taataccaat agctggttat ggtgatgatg caggtgttat
     1321 tgctagtgcg ttggcttcga tctctgcatt cgtaagaaaa gaacattata aaaaggcaga
     1381 tgaattttta tcataataat gtagcgagct atgtggggca gataatggat gtaggtaatg
     1441 gtcttatctg cctcaacaca aattttcaaa aattttgttt ataaaatatt caagtaatat
     1501 tataggggag attaaaatga aaaataattt aatagtatta ttaattgtat taagtaatat
     1561 tttgtttgct cagagttcag attctataaa ggcgagagcg atttttgatt ggattacaga
     1621 taatattgaa tatgatattg aagcctatca aaataataaa gtttctttgg ttcaatgtga
     1681 tgtcgaaaat gttttaaaaa ctaagaaagc tgtttgtgaa ggatacgcaa gattatttca
     1741 aaagatggca caggattcag gtttagaatg caaagtaatt actgggaaat caaaaacatt
     1801 cggttttggt gatcatgcat ggaatgcagt gaaaattgat tccgtctgga ggttggttga
     1861 tgcctgttgg ggagcaggtg gatatagagg taatagattt ataaaatcaa aaaatttatt
     1921 ttactttttt acaccaccag aaaaactaat tttaacacac tttcctgatt ttcaagaata
     1981 tcaatttttg aaaaatccag ttacaaggca agaatttttt gggtctgaaa atactggtta
     2041 ttcacctgta atgtatacct tgctcttagc tgaagtttct gattactctt atgcagaaat
     2101 gatagctatg aatgttgtta caggttatgg tagagcagtt attacttatg atccagcaac
     2161 aaaaaaatat ttggtttact ctggtcaatt tacatcagaa aaagaagcaa ttgaagctag
     2221 caaaactgtt gaaaattgct ctgtaaagaa attagttaaa tgattatgct aaaatttaaa
     2281 tcata
//
LOCUS       DAOTJA010000079         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_43016_length_2084_cov_3.998029, whole genome shotgun sequence.
ACCESSION   DAOTJA010000079 DAOTJA010000000
VERSION     DAOTJA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43016_length_2084_cov_3.998029"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1464)
                     /locus_tag="P5241_04110"
     CDS             complement(208..1464)
                     /locus_tag="P5241_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HRV32678.1"
                     /translation="MIYVPFVYFTLLFLYIIKRNKFDVSAYITLLYIITSFFAILIRL
                     FDYQPISRYELSIIPTIVYCGLITLAIYPIYKFNSNQINGIEIKNTKFIDFLIYFYFF
                     SFIILLVAYKEDILFRFTYGDFAEMRGDDELGLKQYPTIIEAFLLPVRVFANTSYIMI
                     FIFFFSIAFLKKSVLLNLMAILGSLPIVIIGILGIDRSKTFYWIIILGLAMVFFWKYI
                     DRKKKIKITPILFSFLLLVVIYMALVTNSRFGESDTGNRGGVITYAGQSYIHFCYFWD
                     NFDNPDGLSTKYLFPATHYWIIGDYEGSVPYQKELHLKTRIDPGVFYSYLGSFLVDSN
                     KPGPFFFTIIYLLLCRLVLRKKQNIIKFNNLFYSFILSLIPACGVISYIFTSYYMTFS
                     IVTLIILSKILNIGREQVMYPKNSTA"
     gene            complement(1461..>2084)
                     /locus_tag="P5241_04115"
     CDS             complement(1461..>2084)
                     /locus_tag="P5241_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32679.1"
                     /translation="QDLVNKHLGAEPNQIAHLFDLVLTYDKGDAEMYGFEYHPTVFSN
                     FPVSIDYTIPESDVYFVGLAKDRLNIVIDIFYKLRAEGLICDFNLSGVALNQQIKEEG
                     LNYIDKMNYIENLKHIRRTNCILEVVQNNAKGSTLRTWEAIMYDKKLLTNNLSIKDDF
                     YYNKQFISLINDGNIDIEFIKQENLYNNPFKKHISPNNLLEFINRML"
BASE COUNT          777 a          318 c          268 g          721 t
ORIGIN      
        1 ggtgaaatgc ctaattcgtg tgcgagatcg gaaacatttt tccgctcgag acttaatttg
       61 acagcatttt ctttaaatgc cttgtcagaa tgtgttcttt gttttctcat accttgacaa
      121 atgtaagctt tttatgctta actttttgtc ccggcaaagt tagtaactcc agtgttcatt
      181 gagttttaaa tggatttttt taaaccatta agctgtacta tttttcggat acataacctg
      241 ctctcttcca atatttagta tttttgatag aattatcaat gttacaatac taaaagtcat
      301 atagtatgac gtaaagatgt atgaaattac gccacatgca ggtattaatg ataatatgaa
      361 gctataaaac aagttattga atttaataat attttgtttt tttcgaagaa ctaatctaca
      421 caagagcaag taaataattg taaaaaaaaa tggaccaggc ttattactat caactaaaaa
      481 tgatcctaga taggaataaa aaacacctgg atctattcta gtctttaaat gtaactcttt
      541 ttgatatggt acagatcctt cataatctcc aattatccag taatgagtag ctggaaaaag
      601 atatttggtt gataatccat ctggattatc aaaattatcc cagaaataac aaaaatgaat
      661 ataagactga cctgcatatg taattacacc ccctctattc cctgtatcac tctcaccaaa
      721 acgcgaatta gtcactaatg ccatataaat aacaacaagt agaagaaagg aaaacaaaat
      781 aggggtgatt tttatttttt tctttctatc tatatatttc caaaagaata ccattgccaa
      841 ccctaatata ataatccagt aaaatgtttt tgaacgatct attcctaaaa ttccaataat
      901 tacaatcggt aatgatccca agattgccat tagattcaat aatactgatt tctttaagaa
      961 agcaatacta aagaaaaaaa tgaaaatcat aatatatgag gtattagcaa aaactctaac
     1021 tgggagcaaa aaagcctcaa tgatagttgg gtattgtttc aatcccaatt catcatctcc
     1081 tctcatttct gcaaaatctc cataagtgaa tcgaaaaaga atgtcttctt tgtatgctac
     1141 taataaaatt atgaacgaaa agaaataaaa ataaatcaaa aaatcaatga atttagtatt
     1201 tttaatttca ataccattta tttgattact attaaattta tatattggat aaattgctaa
     1261 tgtaattaaa ccacaataaa caattgtcgg gataatagaa agttcatacc tagatattgg
     1321 ttgatagtca aaaagccgaa ttaatattgc aaaaaatgag gttattatgt aaagcaacgt
     1381 aatatatgca cttacatcaa atttatttct tttaattata tacaagaaaa gaagtgtaaa
     1441 ataaacaaaa ggtacatata tcatagcatc ctattaataa attcgagtaa attatttgga
     1501 ctaatatgtt tcttaaatgg attattatat aaattttcct gtttaatgaa ttctatatca
     1561 atattgccat cattaataag tgaaataaat tgcttattat aataaaaatc atcttttata
     1621 gataaattat ttgtaagtaa ttttttatca tacattatgg cttcccatgt tctaagagtt
     1681 gaccctttgg cattattctg aacaacttct aaaatgcaat ttgtacgcct aatatgtttc
     1741 aagttttcta tataattcat tttgtcaata taatttaatc cttcttcctt tatttgttga
     1801 ttaagagcaa cacctgaaag attaaaatca catattaaac cttcggccct tagcttataa
     1861 aaaatatcaa ttactatatt taatcggtct tttgcaagac caacgaaata cacatcgctt
     1921 tcaggaatgg tataatcaat actgaccgga aaattagaaa atacagtagg atgatattca
     1981 aaaccataca tttctgcatc tcctttatcg taagttaata ctaaatcaaa caaatgagct
     2041 atttgatttg gctctgcacc taaatgcttg ttgactagat cttg
//
LOCUS       DAOTJA010000080         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_44252_length_2042_cov_4.007046, whole genome shotgun sequence.
ACCESSION   DAOTJA010000080 DAOTJA010000000
VERSION     DAOTJA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44252_length_2042_cov_4.007046"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="P5241_04120"
     CDS             complement(<1..1333)
                     /locus_tag="P5241_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32680.1"
                     /translation="MKKLFAISIITLSIIGHAVNAQPFSQANLQLWLRADSIEIVDGK
                     VARWYDLSPNNHLIQQTNPAYRPTQIISELYSQPSIYFNGTNNYFDGGDILNIGYSSQ
                     TMFVIAKSEKNRGSVLAKSLSGPSVNRYAILFGNNISFFFQDNLERSISTDLIMNKFL
                     YINTIIDKQINKNFFNVNNDFVGEKTIAANYNMTSTYNFLIGAYNNNTGGVPPVTSLY
                     FKGEITEILIFDTVLSPIDFTLVENYLLDKYTEKLNIGEDINIDYGFCDTSLTVSDYF
                     TDVLWSTGDTSKTIQVNKSGYYSVSAKDAFGRTQYDTVLVKFPSPNISNSNICLGDSV
                     LYSPNLTGTYSYLWSDLSSGAEKYYSEQGDYWLRIEDENSCFDTVFFSVNVDYFEDSI
                     SLGPDSLSLCTGNAIQLMNGEHLCSSFLWSPSGNTNPTETVSETGWYKLEVY"
     gene            complement(1577..>2042)
                     /locus_tag="P5241_04125"
     CDS             complement(1577..>2042)
                     /locus_tag="P5241_04125"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRV32681.1"
                     /translation="SIILCQLILTKGKSGAFLKLEIIKKTLVLLAMIIGLQFGINEFL
                     WCTVIVLYIALYLNLVYAAKALKTIKKTKFITTLLPSLAISIIMGICVFSINYLQIET
                     PWIELIVMVISGILIYVLLSKLFKLWEYVYLKQLIIDKVKEFKAKYIAKTKR"
BASE COUNT          757 a          290 c          258 g          737 t
ORIGIN      
        1 tataaacttc taatttatac caacctgttt cgcttactgt ttctgtagga ttagtattac
       61 ccgaaggcga ccataaaaaa gatgaacata agtgttcgcc attcattaac tgtattgcat
      121 ttcctgtaca taaagataaa gaatctggac ccaaggaaat actatcttca aaataatcta
      181 catttacaga aaaaaacacg gtatcaaagc aagagttctc atcttctatt ctaagccaat
      241 aatctccttg ctctgaataa tatttttcag ccccagaact caaatcagac cataaataag
      301 aataagtccc tgttaaatta ggagagtata aaacagaatc tcctaaacaa atatttgaat
      361 tagaaatatt tggagaagga aattttacta aaacagtatc atattgagtt cttccaaaag
      421 catctttagc tgaaacagaa taataaccag atttattaac ctgtatagtt tttgaagtat
      481 ctccagttga ccataaaaca tcagtaaaat aatcacttac tgttaaactt gtatcacaaa
      541 aaccataatc aatatttata tcttcgccaa tatttaattt ttctgtatat ttatccaaca
      601 aataattttc aactaaagta aaatctatag gagataaaac tgtatcaaaa attaaaattt
      661 ctgtaatttc tcctttaaaa tacaatgaag taacaggtgg tacccctcct gtattattat
      721 tatatgcacc tataagaaaa ttatatgtag aagtcatatt atagtttgct gctattgtct
      781 tttctccaac aaaatcatta ttaacattga aaaagttttt attaatttgt ttatcaatta
      841 tagtgtttat atataaaaat ttattcataa ttaaatctgt tgatatgcta cgctccaaat
      901 tgtcttgaaa gaaaaaagaa atattgttac caaagagtat agcataacga tttactgatg
      961 gtcctgataa agatttagct aaaacagaac ctctattttt ttcagatttt gctattacaa
     1021 acatagtttg agaagaatat ccaatattta aaatatctcc tccatcaaaa taattatttg
     1081 taccgttaaa ataaattgaa ggttgtgaat aaagttcgct tataatttgt gtgggtctat
     1141 atgcaggatt tgtttgttgt ataagatgat tgtttgggct aaggtcgtac cagcgagcaa
     1201 cttttccgtc aacaatttct atagaatcgg cacgcaacca caattgaaga tttgcttgtg
     1261 aaaacggttg ggcatttaca gcatggccta ttattgataa tgttattata gatatagcaa
     1321 ataatttttt catttgtatt tggtaatgat agctattgat aaaattacat gtttaaaaaa
     1381 ctctttgtta ttatttaagg ccaaaaatat cgacaaatat agtaaataaa ttatgaaatc
     1441 atgtgttttt gttttagtat ttttgtaaaa gcttacttta actagttata tttgttgaga
     1501 ctaaaagttg gtgaatattt tgttataaaa ttactaagaa tttattaatt aacataacat
     1561 tagaattagc actattttat cgcttggttt tagctatata ttttgcttta aattctttaa
     1621 ctttatcaat gataagttgt ttaagataca catactccca caacttgaaa agttttgata
     1681 ataaaacata tattaatatt ccactaataa ccataacaat taattcaatc caaggtgttt
     1741 caatttgaag ataattaata ctaaaaacac aaatacccat tataattgat attgctaaag
     1801 atggtaacaa tgttgttata aacttagtct tttttattgt ttttaatgct tttgctgcgt
     1861 aaactaaatt aagatataga gctatatata agactattac agtacaccaa agaaactcat
     1921 taattccaaa ttgcaatcca attatcattg ccaataaaac gagtgttttt tttattattt
     1981 cgagttttaa aaaagcaccc gacttacctt ttgttaatat aagctgacat aatataattg
     2041 at
//
LOCUS       DAOTJA010000081         2011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_45159_length_2011_cov_4.171268, whole genome shotgun sequence.
ACCESSION   DAOTJA010000081 DAOTJA010000000
VERSION     DAOTJA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45159_length_2011_cov_4.171268"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..803)
                     /locus_tag="P5241_04130"
     CDS             complement(219..803)
                     /locus_tag="P5241_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRV32682.1"
                     /translation="MLNCRYKIVLIFILLTRISYSQNLVIGLSGGINFSKAITDEYKS
                     KKQICPTIKLTSDFLLNEKFYLQFGLGYIQHCYSIDIFPNIKMTFNYIDISLAAKYKY
                     KNFFVAGGPIFNIYINGATHINNYSVDFEKDFSESFLYGLSGEIGFELPLGRNFNLLL
                     QSGYSIGFKDATKSDDTVVKFNGTIFLTGLNYNF"
     gene            949..>2011
                     /locus_tag="P5241_04135"
     CDS             949..>2011
                     /locus_tag="P5241_04135"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5127 domain-containing protein"
                     /protein_id="HRV32683.1"
                     /translation="MYKTLIKLLIGIIVFMNLSFTQDLKEIRPPATPLIAVDPYFSVW
                     STSDKLYSERTKHWTGANNSMLCLVKIDGKNYRIMGWEFNKLEPLEQKSLKVLPTRTV
                     YEFEGAGVNIKLTFLTPSIAYDIDLLSLPITYLIWDVRSIDSKEHSVQIYFDVTAELV
                     VNEPSQKVIWSRSKINDIEAIRFGTAEQPILDKKGDNLRIDWGFLYLCFEKNNYSSSS
                     ILSYNISRENFIKLGALINEDDLRNPRPASDSWPVLSYIYNLENVKEEKRNYIALIYD
                     DIFSIQYFYRNLKPYWSKTYSTNELIKKSISQFQEIYSKCVNFDEELVSDLLKVGGNN
                     YATIASLAYRQGLAANKIAI"
BASE COUNT          759 a          249 c          299 g          704 t
ORIGIN      
        1 aatgcggaac ctgccattca atgccaccaa aggcacccca cattcaagta gtagcttgtt
       61 ttgcctgtca ttctgctact gtagatcaga atcagaaaat tattgataag tcaaaacata
      121 taaatggtaa gttagaagtt tatggttctg taagaacaga ttggtaaaaa atttattttc
      181 atttaggccc tgaatagtat tctattcagg gctttttgtt aaaaattata atttaaacca
      241 gttaaaaaaa tagttccatt gaatttgact acagtatcgt cacttttcgt agcatcttta
      301 aatccaatcg aatatccaga ttgtaataac agattgaaat ttctaccgag gggcaattca
      361 aaacctattt cacctgacaa gccataaaga aaagattcag aaaaatcttt ttcaaaatca
      421 accgaataat tatttatatg agttgctcca tttatataaa tgttaaaaat aggacctcca
      481 gcaacgaaaa aatttttgta tttatatttg gcagctaagg aaatatcaat ataattaaat
      541 gtcattttga tattcgggaa aatatctatt gaatagcaat gttgaatata acctaaacca
      601 aattgtaaat aaaatttttc attaagtaaa aaatcgctag taagttttat tgttgggcat
      661 atttgttttt ttgatttata ctcatctgtt attgctttgc taaaatttat tccaccactt
      721 aaaccaatta ctaaattttg agaataagaa attctggtta gtaaaatgaa aattaaaaca
      781 attttatatc tacaatttaa cataaaaata ttgcatatta gtaaatgttc caagtaatta
      841 tttaaagtat aatattaaat ttatagaaaa tcaataatat tattttttaa ttaaaatact
      901 tgtttgttag aaaatttttt tacaaatttg aaaaaaatag gtgcaattat gtataaaact
      961 ttaataaaat tattaatagg aattattgta tttatgaatt tatcttttac acaagatctt
     1021 aaagagatta gacctcctgc aacaccatta atagcagtag atccttattt tagtgtttgg
     1081 tcaacaagtg ataagcttta tagtgaaaga acaaagcatt ggacgggtgc aaataacagt
     1141 atgctttgct tggtgaaaat tgatggtaaa aattatagaa taatgggatg ggaatttaat
     1201 aagcttgaac ctttggaaca aaaatcttta aaagtattac cgacaagaac agtatatgaa
     1261 tttgaaggag caggtgtaaa tataaaacta acatttctta ccccatctat tgcttatgat
     1321 attgatttat tatctttgcc aataacttat ttgatatggg atgttaggtc catagattca
     1381 aaagagcatt ctgtccaaat ttattttgat gttactgcag aattggtagt gaatgaacca
     1441 tctcaaaaag taatatggtc aagatcaaaa attaatgata ttgaagctat aagatttggt
     1501 actgctgaac aaccaatttt agataaaaag ggtgataatt taagaattga ttggggattt
     1561 ttatatttgt gttttgagaa aaacaactat tcttctagtt caatattatc ttacaatatt
     1621 tcgagagaaa attttattaa acttggtgct ttgattaacg aagatgattt aagaaatcca
     1681 cgtccagcaa gtgattcatg gcctgttctt tcatatattt ataatttaga gaatgtaaaa
     1741 gaagaaaaaa gaaattacat tgctcttatt tatgatgata ttttttcaat acaatatttt
     1801 tacagaaatc taaaacccta ttggtctaaa acctatagta caaatgaact tataaaaaaa
     1861 tcaatatcac aatttcaaga gatttattca aagtgtgtta atttcgatga agagttagta
     1921 agtgatttgt tgaaggtagg cggaaataat tatgcaacta ttgcctctct tgcatataga
     1981 cagggtttag cagctaataa aattgcgatc g
//
LOCUS       DAOTJA010000082         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_47175_length_1949_cov_2.696410, whole genome shotgun sequence.
ACCESSION   DAOTJA010000082 DAOTJA010000000
VERSION     DAOTJA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47175_length_1949_cov_2.696410"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="P5241_04140"
     CDS             complement(<1..383)
                     /locus_tag="P5241_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRV32684.1"
                     /translation="MHHHKINGEGLFGFVNMVLGSNASFIFASSIVVTYLLTICISSV
                     SVIGNNLYLLPDNDNLRIILTLFIIWGVTLIELLGIKISQKIYYYSLVFLIILFLNLI
                     ILGIISFDSQKVNYFLLFFNNSIKT"
     gene            complement(689..1726)
                     /gene="queA"
                     /locus_tag="P5241_04145"
     CDS             complement(689..1726)
                     /gene="queA"
                     /locus_tag="P5241_04145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRV32685.1"
                     /translation="MKLSDFKYPLPRNLIAKYPVSPRDRSRLMVLHRDTQEIELKHFY
                     EIVDYFEEGSCLVMNETKVFPARLIGRKEKTNAKIEVFILRELSKEENIWDVIVDPAR
                     KVRIGNKIYFDDDRFYCEVIDNTTSRGRTVRFNTKDDFFKIINKIGKMPLPHYIKREP
                     TEKDKETYQTVYAKNIGSVAAPTAGLHFTKQLLKKIENKGVKLAYITLHVGLGTFRTV
                     EVEDLTKHRMDSEFYEITPQTAEIINKCIAHKGKIIAVGTSTTRALESSVTTDNFVKP
                     IKGWTDKFIFPPYNFRIVNQLITNFHLPSSTLLMLVAAFADRDFVMKAYKKAIKENFR
                     FYSYGDAMYII"
BASE COUNT          699 a          290 c          221 g          739 t
ORIGIN      
        1 attgttttaa ttgaattatt aaaaaaaagt aaaaaataat ttactttttg actatcaaaa
       61 ctaataattc ctaaaattat caaattgaga aatagtatta ttagaaatac taaggaataa
      121 taataaattt tttgtgatat ttttatccct aataattcaa ttaatgtaac accccaaatg
      181 ataaataaag ttaaaattat tcttagatta tcattatccg gtaatagata taaattattc
      241 ccaataacac ttactgaaga aatacaaatt gttaaaagat aagttacaac tatagaagaa
      301 gcaaaaataa aagaagcatt tgaacctaat accatattta caaagccgaa taagccttca
      361 ccatttattt tatgatgatg caaaatctcg gctatttcaa tatacctggt agatatgtaa
      421 ttataaaata aagttgaaaa gattataaaa aaaattgaat gataacctaa atatttaaag
      481 gattcacctg ttgcataaaa tacagatgac aattgtaata ttagaacagg taatgaaatt
      541 gaaataaaag taaaccacca cctacctgca gaaaattcaa taagaaaatt ttttcttcta
      601 gataatttta ataaaaatac aattattaca agattaaata aaaatgtaaa aaaagtaaca
      661 atcatctatt attttttacg actccttatt aaataatata catcgcatca ccataactat
      721 aaaacctgaa attttctttt attgcttttt tataggcttt cataacaaaa tctctatcag
      781 caaaagcagc cactaacatt aacaaagtag aactcggtaa atgaaaattt gtaattaatt
      841 gattcacaat tctaaaatta taaggtggaa atatgaactt atctgtccac ccttttatag
      901 gttttacaaa attatctgta gttacactgg attcaagagc tctagtggtg cttgttccaa
      961 ctgcaattat tttgccttta tgagcaatac atttattaat aatttctgca gtttgcggag
     1021 ttatttcata aaactcagag tccattctat gttttgttaa atcttcaact tcaaccgttc
     1081 taaatgtacc tagaccgaca tgtagagtaa tataagctaa ttttacacct ttattctcaa
     1141 ttttttttag taattgtttt gtaaaatgca aaccagctgt tggagcagca acggaaccga
     1201 tatttttagc ataaactgtt tgatatgttt ctttatcttt ttcagttggt tctcgtttaa
     1261 tataatgtgg taaaggcatt ttaccgattt tattaataat tttaaagaaa tcatcttttg
     1321 tgttaaaacg aactgttctt cctctagatg tagtattatc aataacttca caataaaacc
     1381 tatcatcatc aaagtatatt ttgttcccga ttcttacttt tcttgcggga tcaacaataa
     1441 catcccatat attctcttcc ttgctaagtt ctctaagaat aaagacttca atctttgcat
     1501 ttgttttttc ttttcttcca atcaatctag cagggaatac ttttgtttca ttcattacaa
     1561 gacaactacc ttcttcaaaa taatcaacaa tttcgtagaa atgttttaat tcgatctctt
     1621 gagtatccct gtgtaaaacc attaatcttg acctatctct tggtgaaact ggatattttg
     1681 ctattaaatt ccttggtaaa ggatatttaa agtcagataa tttcatattt ttttctcctt
     1741 cacactaatt atcaattata aaactaaggt tggagataaa aatctccaac ctatcttaat
     1801 tagttattgt aatattatct ttttttctta gaaacaactt tctttgtttt gtttttatta
     1861 gttgtttttt taacagctac ttttttgata acagcctttt tagtagttgc tttttttgct
     1921 atagcttttt ttgaacttgc ttttttaac
//
LOCUS       DAOTJA010000083         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_47396_length_1942_cov_3.697403, whole genome shotgun sequence.
ACCESSION   DAOTJA010000083 DAOTJA010000000
VERSION     DAOTJA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47396_length_1942_cov_3.697403"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="P5241_04150"
     CDS             <1..626
                     /locus_tag="P5241_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV32686.1"
                     /translation="LWVMLDAAHPVGQFPIDVKDIDCDFYVSCGHKWMMGPKGTGFLF
                     MKKSMYEIVDPLFVGAGSVTDWDYKKGIHEYVNTGHRYDYTTRSAALSVGLEAAADFL
                     LSIGMNNVGQRSRALATYLMNNIKDLPNIEMLTPFEDKSRAALVGFRFKNIEYTKLQN
                     WLMEHYKIRVRGVPESGLNSLRISTHIYNDFDDVNKLIEALKKASTL"
     gene            672..1316
                     /locus_tag="P5241_04155"
     CDS             672..1316
                     /locus_tag="P5241_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRV32687.1"
                     /translation="MNKQIIILIILTILSFIFWNTIVVYPVKLFVVLLHELSHGLAAI
                     LTGGKIIKVEISYLIGGSCTTQGGSPSLIANAGYLGSILLGGLLLVHSSKSKNIKFLG
                     LFLSLSIFLITIFYIRNSFGIIFGISFSFILFLLTFILPKKILEWILKFIGLVSCFYV
                     LIDIKEDLFSNPPKGTDANLLYQITGINAIFWAVLWGILALIAIIFFLRKSISK"
     gene            1423..>1942
                     /locus_tag="P5241_04160"
     CDS             1423..>1942
                     /locus_tag="P5241_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32688.1"
                     /translation="MKRFYFFCFIIILNTNFAISQNDKLIYYNIFKEENSKYRWFNCS
                     NLIYKYISYNKWSADKVKDLIDILSSDEDKINATIGEYWRNHWIQKSDEIKENAQKEF
                     DRNNTQRGNQLILDAKFAEKISTSINDIYITNYTIHYILNKKIRPQGNSLNLPKEQNL
                     QNFRKYYAVNYDE"
BASE COUNT          711 a          230 c          288 g          713 t
ORIGIN      
        1 gtctttgggt aatgcttgat gctgctcatc cagtaggtca attccctata gatgtaaaag
       61 atattgattg tgatttttat gtttcttgtg gacataaatg gatgatgggt cctaaaggaa
      121 ctggtttctt attcatgaaa aaaagcatgt atgaaattgt tgatcctctt tttgttggag
      181 cagggtctgt tactgactgg gactataaga aaggtatcca tgaatatgtt aataccgggc
      241 atagatatga ttacacaact agaagcgctg ctcttagtgt tgggcttgaa gcagctgcag
      301 attttctact tagtattggt atgaataatg ttggtcaaag gtcaagagca ttagcaacct
      361 atttaatgaa taacatcaaa gacttaccaa atattgaaat gttaactcca tttgaagata
      421 aatcaagagc tgcattagtt ggttttcgtt ttaaaaatat tgagtataca aaattacaaa
      481 actggctaat ggaacattat aaaattagag taagaggggt tcccgaaagc ggtttaaatt
      541 ctcttaggat atctactcat atttataatg attttgatga tgtaaacaaa ttaattgaag
      601 cattaaaaaa agccagtaca ttataatttc aaaaagtgag ctatttatat atagctcact
      661 tttttcttaa aatgaataaa caaataataa ttttaattat cctaactatt ttatctttta
      721 tattctggaa tacaatagtt gtatatccag tcaaattatt tgtagtattg ttgcatgaac
      781 tttcacatgg tttagcagca attttaactg gtggaaaaat aatcaaagta gaaattagtt
      841 acttaattgg tggtagttgc acaactcaag gtgggagtcc ctccttaatt gctaatgcag
      901 gataccttgg tagtatactt cttggaggat tattattagt tcattcttca aaatcgaaaa
      961 acatcaaatt tttaggttta tttctttcac tctcgatatt tttaatcaca attttttaca
     1021 ttagaaattc ttttggtata atttttggaa taagttttag ctttatttta tttttattaa
     1081 cttttattct tccaaaaaaa attttagaat ggattttaaa atttattggt ttagtatcct
     1141 gtttttatgt gttgatagac ataaaagaag atttattttc taatcctcca aagggtactg
     1201 atgcaaattt attatatcaa ataacaggaa taaacgcaat attttgggca gttttatggg
     1261 gtattttagc cttaattgct ataatatttt ttttgagaaa gagtatttcc aaataagttt
     1321 attcatatta tcacatattt tttccgataa taatataaat tctgaaaata attgattatt
     1381 ttttaaactt tgattaaatt aaataaattt ttgactttgt ctatgaaaag attttatttt
     1441 ttttgtttta taataatact caacacaaat tttgcaattt cacaaaatga taaactaatc
     1501 tattataata tctttaaaga ggaaaattca aaatatcgtt ggtttaactg ttcaaattta
     1561 atttataaat atatttctta taacaaatgg agtgcagata aagtcaagga tttgatcgat
     1621 atattatcaa gtgatgaaga taaaattaac gctacaattg gtgaatattg gagaaaccat
     1681 tggattcaaa aatcagatga aataaaagaa aatgcccaga aagaattcga tagaaacaac
     1741 actcaaagag gcaatcaatt aatacttgac gctaaatttg cagaaaaaat atcaacttct
     1801 ataaatgata tttatattac aaattataca attcattata tattaaataa aaaaattaga
     1861 cctcaaggaa attcacttaa tttaccaaaa gaacaaaatt tacaaaattt cagaaaatac
     1921 tatgctgtaa attatgatga aa
//
LOCUS       DAOTJA010000084         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_48151_length_1920_cov_4.033244, whole genome shotgun sequence.
ACCESSION   DAOTJA010000084 DAOTJA010000000
VERSION     DAOTJA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48151_length_1920_cov_4.033244"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="P5241_04165"
     CDS             complement(<1..329)
                     /locus_tag="P5241_04165"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HRV32689.1"
                     /translation="MDKMKNFFKLAVLTIFFFSFHSLLFTQNKNINPFSKTNFVPSQK
                     YYSFGNWLEDTLGNQRVLINVKNKSSVVYICVPWRRSDQNPSKKGIMITYAKTNEEVK
                     NIYVKEI"
     gene            complement(358..507)
                     /locus_tag="P5241_04170"
     CDS             complement(358..507)
                     /locus_tag="P5241_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV32690.1"
                     /translation="MGEVFWLILTVACVVWYSTITFYVAIKGVGDIKNILKSLKSNID
                     ENKTD"
     gene            complement(509..>1920)
                     /locus_tag="P5241_04175"
     CDS             complement(509..>1920)
                     /locus_tag="P5241_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV32691.1"
                     /translation="TMIAGIMVRKYVGKVDHFLLAGREMNVYLGIASLAATEFGVVTC
                     MYTAQNGYELGFSGATPGIIMALSMLIVGYTGFCIKPLRNSGVITIPELFQKKFGTKI
                     RWAAGVVIVLGGLLNMGVFLRTGGEFLVMVTGLNIHYLEITMTILLIAVAIYTILGGM
                     LSVLVTDFLQFVVMSLGLISVTVLILINVGWSNLTATVTSHYGAGGFNPFVHPKMGWP
                     YVLFNVFLNFSVVLTWQTIISRLLAAKDSKTGSKIYKGTSFFFVARFLIPGIWGIAAL
                     TVLGTDITGGSSLYAMPQLLNKILPVGLMGVLVAAMLAADMSTDSSYMLTWGSVIYND
                     ILAPFRKTKWSEKKGLFWNRTIVAIIGVFLLFYGLWYPLKGDLWTYLGVTGTIYQASI
                     STLLISACYWKKANSWGAAGAIIVGAAFPITFLVLEQLPMTASFAKSIGPYYSGIAAY
                     IFSGLAMIIGSLMKPKKED"
BASE COUNT          702 a          370 c          239 g          609 t
ORIGIN      
        1 tttatttctt ttacataaat attttttact tcctcatttg ttttagcata agtaatcatt
       61 attccttttt tagagggatt ttgatcgctt cttcgccaag gaacacaaat ataaactaca
      121 gaagatttat ttttaacatt aataagtact ctttgatttc caagagtatc ctctaaccaa
      181 ttaccaaaac tataatattt ttgtgaagga acaaaattag tttttgagaa aggatttata
      241 tttttatttt gagtaaataa taacgaatga aaactaaaaa aaaatattgt taatacagca
      301 agtttaaaaa aattcttcat tttatccatt attattaaaa aattatttaa aaataaatta
      361 atcagtctta ttttcatcta tattgctttt taaagatttc aaaatgtttt taatatcacc
      421 tacaccttta atggctacat aaaaagtgat agtagaatac cacaccacac aagctactgt
      481 aagtataagc caaaaaacct ctcccatttt aatcctcctt tttaggtttc attaatgaac
      541 caataatcat agccagacca gagaaaatat atgctgcaat acctgaataa tatggaccaa
      601 tagatttagc aaaagatgct gtcattggta attgttctaa taccaaaaat gttattggga
      661 aagcagcacc aactattatg gctccggcag caccccagct atttgccttc ttccaataac
      721 aagcagatat aagcaaagta gaaatacttg cttgatagat agttccggta acaccaagat
      781 aagtccataa atctcctttc aatggatacc ataaaccata gaaaagtaaa aatactccta
      841 taattgctac aattgttcta ttccagaaaa gacctttttt ttctgaccac ttggtttttc
      901 taaagggtgc taaaatatca ttgtatatta cgcttcccca tgtaagcata tatgatgaat
      961 ctgttgacat atctgctgca agcatcgcag caaccagcac tcccattaaa ccaacaggta
     1021 gtattttatt taacaactgt ggcattgcat ataagctact acctcctgtt atatcagtac
     1081 caagaacagt taacgcagct ataccccata ttccgggaat taaaaatctt gcaacaaaaa
     1141 agaaactagt gcctttataa atttttgaac cagttttaga atcttttgct gctaataacc
     1201 ttgaaattat tgtttgccac gttaaaacaa cagaaaaatt taagaataca ttgaatagca
     1261 cataaggcca acccatttta ggatgaacaa aaggattaaa ccctccagct ccataatgcg
     1321 atgttactgt tgcagttaaa ttactccacc ctacatttat taaaattaaa actgtaacac
     1381 taataagccc taaactcata acaacaaatt gcaaaaaatc tgttacaagc acagataaca
     1441 ttccacctaa aattgtatat attgctacag caattaaaag tattgtcatt gttatttcaa
     1501 gatagtgaat atttagccct gtaaccatta ctaaaaattc acctcctgtt ctaagaaaaa
     1561 ctcccatatt caaaagacca cctaatacaa taacaactcc agcagcccat cttatcttag
     1621 taccaaattt tttttgaaat aactctggta ttgtaattac tccagagttc cttagtggtt
     1681 ttatacaaaa accagtatag cctactataa gcatcgataa agccataatt ataccaggtg
     1741 tggctccaga aaaacccagc tcataaccat tttgagcagt gtacatacaa gttacaacgc
     1801 caaactcagt agcagctaaa gaggcgatac caaggtatac gttcatttct ctaccagcaa
     1861 gtagaaaatg atctacttta cctacatatt ttctgaccat aattcctgcg atcattgtac
//
LOCUS       DAOTJA010000085         1413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_74896_length_1413_cov_2.672312, whole genome shotgun sequence.
ACCESSION   DAOTJA010000085 DAOTJA010000000
VERSION     DAOTJA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74896_length_1413_cov_2.672312"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="P5241_04180"
     CDS             complement(<1..1367)
                     /locus_tag="P5241_04180"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRV32692.1"
                     /translation="MSLIELDDLDRSILQSAVDLKSKFVSSQEAIDTNIIIINVETAN
                     PVPRGEIAKAIKFLKDNGAKTIALNVFLDEPRDDIDPQWDSDLAEAIEYKTQNVIGIN
                     GFQYFVNDKNEIDTMLSLNPIDKFNKMKAVGFGNLEMTPENEGSIVQQFYPNAYFKGK
                     PLKSFPLAVAEHYDSSRAKILSNKFDKEYVNFYKKELFKVWNLSFLTNPIINNAFAPL
                     IKDKLFIIGYINPESDPLKFNMYSTPIGKLEHIFVYAGIISDILTHKFVPESPFIVDM
                     IVGVIFCLLNIMLFSIASKKTSLWEKFTGVTLIPLEIVFIYIVVIFVFVFFNYKLSVV
                     IPSIIIIISIPLNSYTHKKILPLMQKYYSIFKYRNIPYPFLQQYLNIFIHEKRSIRMF
                     TTIFGIQRVLWLLNAFSMIEVIQKTSRKIDNNLFDTLNIKSWDDLYKFVSENKNLPIN
                     AIIYS"
BASE COUNT          477 a          207 c          192 g          537 t
ORIGIN      
        1 atactatata taattgcatt tataggcagg tttttatttt cggatacaaa tttatataaa
       61 tcatcccaac tttttatatt aagggtgtca aataaattgt tatcaatttt tctcgatgtt
      121 ttttgaatta cctcaatcat agagaaagca tttagtagcc agagtaccct ttgaatacca
      181 aatatagtag taaacattct tatcgatctt ttctcatgta taaaaatgtt taaatattgt
      241 tgtaaaaaag gatagggtat atttctatat ttaaaaattg aatagtactt ttgcatcaat
      301 ggtaaaatct ttttgtgtgt ataactgttt aaaggaatag atattataat tattatactg
      361 ggtattacta ccgataactt ataattaaag aaaacaaaaa caaatattac cactatataa
      421 ataaaaacaa tttctaatgg aataagagtt acacctgtaa acttttccca taatgatgtt
      481 tttttactgg caattgaaaa aagcattata ttcaaaaggc aaaatattac tcctactatc
      541 atatctacaa taaaaggaga ttctggtaca aatttatgag ttagaatatc acttattatg
      601 ccagcataga caaaaatatg ttcgagcttt ccaataggtg tgctatacat attaaatttt
      661 aacgggtcag attctgggtt gatatatccg ataataaaaa gcttatcttt tattaaaggt
      721 gcaaaagcat tgttgattat tggattagta agaaaactta aattccatac tttgaatagt
      781 tcttttttat aaaagttaac atattcttta tcaaatttat tgctaagtat ctttgctctg
      841 ctgctatcat aatgttcagc aactgcaaga gggaatgatt ttaagggttt acctttaaaa
      901 tatgcattag ggtaaaactg ctggacaatg ctaccttcat tttctggcgt catttcaagg
      961 tttccaaagc caactgcctt cattttatta aatttatcta ttggatttaa tgatagcatt
     1021 gtatctattt catttttatc attcacaaaa tattgaaagc cattaattcc tataacattt
     1081 tgagttttgt attcaatggc ttcggcaaga tcactatccc attgtggatc aatatcatct
     1141 cttggttcat ctaaaaaaac atttaacgct attgttttag ctccattatc cttcaaaaac
     1201 tttatggctt ttgcaatctc tccacgaggt acaggatttg cagtctccac attaattatt
     1261 attatatttg tatcaatagc ttcttggctt gaaacaaatt tcgattttaa atctactgca
     1321 gattgaagta tgcttctatc taagtcatct aattcaatga ggctcatgtt tttaaagact
     1381 tctggtactg gaattatctc taatcctttt tgt
//
LOCUS       DAOTJA010000086         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_77417_length_1382_cov_3.155991, whole genome shotgun sequence.
ACCESSION   DAOTJA010000086 DAOTJA010000000
VERSION     DAOTJA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77417_length_1382_cov_3.155991"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="P5241_04185"
     CDS             complement(<1..514)
                     /locus_tag="P5241_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRV32693.1"
                     /translation="MNFLYIIFSFLLFFSTTNWDTFRGNHQHTGFITTKENLPTLEKA
                     LWKYKTNGDVYSSPVIIDSKVYFGSNDCNFYCITADEGRLVWKKELGNLIMSSPIYYK
                     GKIIVGCDDGCLYQLNYKTGEVISKFQSNGRIRSASVIYKDNIIFTSRDNHCYSITLD
                     SSKLIWSYKTR"
     gene            900..>1382
                     /locus_tag="P5241_04190"
     CDS             900..>1382
                     /locus_tag="P5241_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV32694.1"
                     /translation="MSLDIREVNELIQRESAFVDLLTMEIGKVIVGQKNMVEKLLIGL
                     LGDGHILLEGVPGLAKTMAIKTLAHTIRARFQRIQFTPDLLPADLIGTMIYNQKEGTF
                     QTKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGEKTYLLEEPFLVLATQNP
                     I"
BASE COUNT          523 a          179 c          194 g          486 t
ORIGIN      
        1 ttcgcgtttt gtaagaccaa attaattttg aagaatcaag agtaatagaa taacaatgat
       61 tatctctact tgtaaaaatt atgttatctt tatatattac agaagctgat cttattctgc
      121 cattactctg aaattttgaa ataacttcac ccgttttata attcaattga tataaacagc
      181 catcatcaca accaacaata atttttcctt tgtaatagat tggagatgac attatcaggt
      241 tgccaagttc ttttttccac actaatctac cctcatcagc tgtgatacaa taaaaattgc
      301 aatcattaga accaaaataa acttttgaat cgattattac aggtgaagaa tatacatctc
      361 catttgtttt atatttccat aaggcttttt ctaatgttgg taaattttct tttgtagtaa
      421 taaaaccagt atgttgatga ttacctcgaa aagtatccca attggtagtt gaaaagaata
      481 ataaaaaact aaatattata tatagaaaat tcattattat acccaatttg aaatattaat
      541 ttactcattt atatatataa aatcaatgtt ataaagattg acttaataac atatcaaatg
      601 attattggtt tatacttagc aaaatttatt taaaactctg gatgatagta ttctgtttgt
      661 atttaaaata ttaattttct ataaaccaca ttattttatg tgaaaaaagg gtgattgaat
      721 tttaaaaatt tagcaaaata cttatacttc agtttttttt attaaattgt cattttaaaa
      781 aacctttcat actagaattt gtaatataga attaaaaaca ttaaataaaa ttgcattgat
      841 ttttttaatg gaaagcttaa attaagtatt tctgaaaatt agctttaata ggaggtataa
      901 tgtctttaga tataagagaa gttaatgagc taatccaacg agaaagtgct tttgtagatc
      961 ttttgactat ggagattggc aaagtaatag ttggtcaaaa gaatatggtt gaaaagttac
     1021 ttataggttt gttaggagat ggacatatat tattagaagg tgtgccaggt ttggcaaaaa
     1081 ctatggcaat aaaaaccctt gctcacacaa ttagagcaag atttcagaga attcaattta
     1141 caccagatct tttgcccgct gatttaattg gtacaatgat atacaatcaa aaagaaggaa
     1201 catttcaaac aaaaaaaggt ccaatatttg ctaattttat tttagcagat gaaatcaaca
     1261 gagctcctgc aaaagtccaa agtgctctcc ttgaagctat gcaagaaaga caggtgacaa
     1321 taggagaaaa aacatattta ttggaagagc cttttttagt attggctact caaaatccaa
     1381 ta
//
LOCUS       DAOTJA010000087         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3011
            NODE_83086_length_1319_cov_2.825158, whole genome shotgun sequence.
ACCESSION   DAOTJA010000087 DAOTJA010000000
VERSION     DAOTJA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868035
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:40:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 840
            CDSs (total)                      :: 797
            Genes (coding)                    :: 702
            CDSs (with protein)               :: 702
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 0 of 95
            Pseudo Genes (incomplete)         :: 35 of 95
            Pseudo Genes (internal stop)      :: 71 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83086_length_1319_cov_2.825158"
                     /isolate="AMR_MDS_3011"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="P5241_04195"
     CDS             <1..268
                     /locus_tag="P5241_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV32695.1"
                     /translation="KIMQSKLSDIKQIEYYTPDSGLYFFVDFSKYGNDILLAEKILEY
                     ANVITVPGSAFGENGKGFLRLSFGAEPEKIIIGLEKIKKYLETI"
     gene            279..1256
                     /locus_tag="P5241_04200"
     CDS             279..1256
                     /locus_tag="P5241_04200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRV32696.1"
                     /translation="MSSYKKVCEVISKTCLDINKGENVVIVTDINKQKIGNELFNYLQ
                     NITNTVMIQIPVTSKNGEEPVAIVNNIFQKADVIIAPTTMSLSHTRAFVNARKKGTRV
                     ASLPGITENMFLQSIDVEYSAMSELTKKLADAIKGKNIIKVKTDLGTDVSFSVKGRKP
                     ICLDGICHKKGGFINLPDGEMCIAPIENSMNGVLVFDLSSSPDHRTKWGKVGLLNNEK
                     IKLVIKNGKIISFSNGAKAKIIKNIIDNSDKNANCIAEFAIGTNNKARITGLILEDEK
                     KLGTIHFAFGSNVSIGGKNQSNLHLDMIMDIPDVFFDNFQVLKKGKLLI"
BASE COUNT          525 a          128 c          221 g          445 t
ORIGIN      
        1 aaaaattatg caatcaaagc tttcagatat taaacaaata gagtattata caccagattc
       61 tggattatat ttcttcgtag atttttcaaa atatggtaat gatattttat tagcagaaaa
      121 aatattagag tatgcgaatg taataactgt acctggttca gcttttgggg aaaatggaaa
      181 aggattttta cgtctatcat ttggtgctga accagaaaaa attattatag gtttagagaa
      241 aataaaaaaa tatttagaaa ctatttgaaa gtgatactat gagttcatat aaaaaagtat
      301 gtgaagttat atcaaaaact tgtttagata ttaataaagg cgaaaatgtt gttatagtta
      361 cagatattaa taaacaaaaa attggcaatg aattatttaa ttatcttcaa aatattacta
      421 atactgttat gattcaaata cctgttacat caaagaatgg tgaagaacct gtagcaattg
      481 taaataatat ttttcaaaaa gccgatgtaa taatagcacc aactacgatg tcattgagcc
      541 atacaagagc ttttgttaat gcaagaaaaa aaggtacaag agtagcttca ttaccgggta
      601 taactgaaaa catgttttta caatctatag atgtagaata ttctgctatg agtgaactta
      661 caaaaaaatt ggctgatgca ataaaaggta aaaacattat taaagttaaa acagatcttg
      721 gaaccgatgt atcattttca gttaaaggga gaaaacccat ttgtcttgat ggtatttgtc
      781 ataagaaagg tggatttatt aatttaccag atggtgaaat gtgtattgcc cccattgaaa
      841 atagtatgaa tggagtatta gtttttgatt taagctcgag tcctgatcat agaactaaat
      901 ggggaaaagt tggtttatta aataatgaaa aaattaagtt agtaattaag aatgggaaaa
      961 taatttcgtt tagtaatgga gcaaaagcta aaattataaa aaatattatt gacaattctg
     1021 ataaaaatgc taattgcatt gctgagtttg ctataggcac aaataataaa gcaagaatta
     1081 caggtcttat tttagaagat gagaaaaaat taggaacaat acattttgcc tttggaagca
     1141 atgttagtat cggagggaaa aatcaaagta atttacattt agatatgata atggatatac
     1201 ctgatgtttt ttttgataat tttcaggttt tgaaaaaggg taagttactt atataaataa
     1261 tataattatt aaatatgata ttaaaatatt agttttattt ttctttttat atcttagat
//
